LOCUS       DBEJAQ010000001       145224 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.001, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000001 DBEJAQ010000000
VERSION     DBEJAQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 145224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 145224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..145224
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.001"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..585
                     /locus_tag="ACL297_000001"
     CDS             <1..585
                     /locus_tag="ACL297_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGR0070338.1"
                     /translation="MPSVIANYLVYVVLALYGSFLFKDRLIQQWKEIRKTKRKFFFGV
                     LTGWLFLILMTVVFEFVSEMLKQFVRLDGQGLNQSNIQSTFQEQPLLIAVFACVIGPL
                     VEELFFRQVLLHYLQERLPGLLSIILVGLVFALTHMHSLALSEWIGAVGYLGGGLAFS
                     IIYVKEKENIYYPLLVHMLSNSLSLIILAISIVK"
     gene            complement(722..1753)
                     /locus_tag="ACL297_000002"
     CDS             complement(722..1753)
                     /locus_tag="ACL297_000002"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HGR0070339.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(2008..2691)
                     /locus_tag="ACL297_000003"
     CDS             complement(2008..2691)
                     /locus_tag="ACL297_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HGR0070340.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILTSMFLWLLVFFATSFLPASLNPVLDPLPLAIIGVALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(2703..3647)
                     /locus_tag="ACL297_000004"
     CDS             complement(2703..3647)
                     /locus_tag="ACL297_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGR0070341.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            3779..6610
                     /gene="uvrA"
                     /locus_tag="ACL297_000005"
     CDS             3779..6610
                     /gene="uvrA"
                     /locus_tag="ACL297_000005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGR0070342.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            6603..7664
                     /locus_tag="ACL297_000006"
     CDS             6603..7664
                     /locus_tag="ACL297_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGR0070343.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            7796..8194
                     /gene="spx"
                     /locus_tag="ACL297_000007"
     CDS             7796..8194
                     /gene="spx"
                     /locus_tag="ACL297_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HGR0070344.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            8260..8829
                     /locus_tag="ACL297_000008"
     CDS             8260..8829
                     /locus_tag="ACL297_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP0191 family lipoprotein"
                     /protein_id="HGR0070345.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            8916..9182
                     /locus_tag="ACL297_000009"
     CDS             8916..9182
                     /locus_tag="ACL297_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGR0070346.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            9186..9605
                     /gene="ruvX"
                     /locus_tag="ACL297_000010"
     CDS             9186..9605
                     /gene="ruvX"
                     /locus_tag="ACL297_000010"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGR0070347.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            9621..9926
                     /locus_tag="ACL297_000011"
     CDS             9621..9926
                     /locus_tag="ACL297_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGR0070348.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            10184..11434
                     /locus_tag="ACL297_000012"
     CDS             10184..11434
                     /locus_tag="ACL297_000012"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGR0070349.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            11518..11973
                     /locus_tag="ACL297_000013"
     CDS             11518..11973
                     /locus_tag="ACL297_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0198 family lipoprotein"
                     /protein_id="HGR0070350.1"
                     /translation="MKLKRFTLSLASLASFSLLVACSQRAQQVQQPVAQQVQQPAQQN
                     TNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDGDRITLVVTGTTGTWTELESDG
                     DQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            12119..13651
                     /gene="cls"
                     /locus_tag="ACL297_000014"
     CDS             12119..13651
                     /gene="cls"
                     /locus_tag="ACL297_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGR0070351.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            13909..15447
                     /locus_tag="ACL297_000015"
     CDS             13909..15447
                     /locus_tag="ACL297_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HGR0070352.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            15567..17774
                     /gene="nrdD"
                     /locus_tag="ACL297_000016"
     CDS             15567..17774
                     /gene="nrdD"
                     /locus_tag="ACL297_000016"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGR0070353.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            17786..17926
                     /locus_tag="ACL297_000017"
     CDS             17786..17926
                     /locus_tag="ACL297_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070354.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            17970..18476
                     /locus_tag="ACL297_000018"
     CDS             17970..18476
                     /locus_tag="ACL297_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070355.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            18469..19059
                     /gene="nrdG"
                     /locus_tag="ACL297_000019"
     CDS             18469..19059
                     /gene="nrdG"
                     /locus_tag="ACL297_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGR0070356.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            19067..19684
                     /locus_tag="ACL297_000020"
     CDS             19067..19684
                     /locus_tag="ACL297_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGR0070357.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRYASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            19950..20258
                     /gene="rpsJ"
                     /locus_tag="ACL297_000021"
     CDS             19950..20258
                     /gene="rpsJ"
                     /locus_tag="ACL297_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGR0070358.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            20475..21101
                     /gene="rplC"
                     /locus_tag="ACL297_000022"
     CDS             20475..21101
                     /gene="rplC"
                     /locus_tag="ACL297_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGR0070359.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            21126..21749
                     /gene="rplD"
                     /locus_tag="ACL297_000023"
     CDS             21126..21749
                     /gene="rplD"
                     /locus_tag="ACL297_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGR0070360.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            21749..22045
                     /locus_tag="ACL297_000024"
     CDS             21749..22045
                     /locus_tag="ACL297_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGR0070361.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            22063..22896
                     /gene="rplB"
                     /locus_tag="ACL297_000025"
     CDS             22063..22896
                     /gene="rplB"
                     /locus_tag="ACL297_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGR0070362.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            23000..23281
                     /gene="rpsS"
                     /locus_tag="ACL297_000026"
     CDS             23000..23281
                     /gene="rpsS"
                     /locus_tag="ACL297_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGR0070363.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            23293..23637
                     /gene="rplV"
                     /locus_tag="ACL297_000027"
     CDS             23293..23637
                     /gene="rplV"
                     /locus_tag="ACL297_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGR0070364.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            23650..24303
                     /gene="rpsC"
                     /locus_tag="ACL297_000028"
     CDS             23650..24303
                     /gene="rpsC"
                     /locus_tag="ACL297_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGR0070365.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            24307..24720
                     /gene="rplP"
                     /locus_tag="ACL297_000029"
     CDS             24307..24720
                     /gene="rplP"
                     /locus_tag="ACL297_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGR0070366.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            24730..24936
                     /gene="rpmC"
                     /locus_tag="ACL297_000030"
     CDS             24730..24936
                     /gene="rpmC"
                     /locus_tag="ACL297_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGR0070367.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            24961..25221
                     /gene="rpsQ"
                     /locus_tag="ACL297_000031"
     CDS             24961..25221
                     /gene="rpsQ"
                     /locus_tag="ACL297_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGR0070368.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            25247..25615
                     /gene="rplN"
                     /locus_tag="ACL297_000032"
     CDS             25247..25615
                     /gene="rplN"
                     /locus_tag="ACL297_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGR0070369.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            25693..25998
                     /gene="rplX"
                     /locus_tag="ACL297_000033"
     CDS             25693..25998
                     /gene="rplX"
                     /locus_tag="ACL297_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGR0070370.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            26022..26564
                     /gene="rplE"
                     /locus_tag="ACL297_000034"
     CDS             26022..26564
                     /gene="rplE"
                     /locus_tag="ACL297_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGR0070371.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            26582..26851
                     /gene="rpsN"
                     /locus_tag="ACL297_000035"
     CDS             26582..26851
                     /gene="rpsN"
                     /locus_tag="ACL297_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGR0070372.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            27136..27534
                     /gene="rpsH"
                     /locus_tag="ACL297_000036"
     CDS             27136..27534
                     /gene="rpsH"
                     /locus_tag="ACL297_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGR0070373.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            27726..28262
                     /gene="rplF"
                     /locus_tag="ACL297_000037"
     CDS             27726..28262
                     /gene="rplF"
                     /locus_tag="ACL297_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGR0070374.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            28346..28702
                     /gene="rplR"
                     /locus_tag="ACL297_000038"
     CDS             28346..28702
                     /gene="rplR"
                     /locus_tag="ACL297_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGR0070375.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            28720..29214
                     /gene="rpsE"
                     /locus_tag="ACL297_000039"
     CDS             28720..29214
                     /gene="rpsE"
                     /locus_tag="ACL297_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGR0070376.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            29228..29410
                     /gene="rpmD"
                     /locus_tag="ACL297_000040"
     CDS             29228..29410
                     /gene="rpmD"
                     /locus_tag="ACL297_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGR0070377.1"
                     /translation="MAQIKITLTKSPIGRIPSQRKTVVALGLGKLNSSVIKEDNAAIR
                     GMITAVSHLVTVEEVN"
     gene            29555..29995
                     /gene="rplO"
                     /locus_tag="ACL297_000041"
     CDS             29555..29995
                     /gene="rplO"
                     /locus_tag="ACL297_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGR0070378.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            30008..31318
                     /gene="secY"
                     /locus_tag="ACL297_000042"
     CDS             30008..31318
                     /gene="secY"
                     /locus_tag="ACL297_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGR0070379.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            31469..32107
                     /locus_tag="ACL297_000043"
     CDS             31469..32107
                     /locus_tag="ACL297_000043"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGR0070380.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            32224..32442
                     /gene="infA"
                     /locus_tag="ACL297_000044"
     CDS             32224..32442
                     /gene="infA"
                     /locus_tag="ACL297_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGR0070381.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            32467..32583
                     /gene="rpmJ"
                     /locus_tag="ACL297_000045"
     CDS             32467..32583
                     /gene="rpmJ"
                     /locus_tag="ACL297_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGR0070382.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            32601..32966
                     /gene="rpsM"
                     /locus_tag="ACL297_000046"
     CDS             32601..32966
                     /gene="rpsM"
                     /locus_tag="ACL297_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGR0070383.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            32984..33367
                     /gene="rpsK"
                     /locus_tag="ACL297_000047"
     CDS             32984..33367
                     /gene="rpsK"
                     /locus_tag="ACL297_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGR0070384.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            33410..34345
                     /locus_tag="ACL297_000048"
     CDS             33410..34345
                     /locus_tag="ACL297_000048"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGR0070385.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            34357..34743
                     /gene="rplQ"
                     /locus_tag="ACL297_000049"
     CDS             34357..34743
                     /gene="rplQ"
                     /locus_tag="ACL297_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGR0070386.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            35008..35274
                     /locus_tag="ACL297_000050"
     CDS             35008..35274
                     /locus_tag="ACL297_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGR0070387.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            35284..36621
                     /locus_tag="ACL297_000051"
     CDS             35284..36621
                     /locus_tag="ACL297_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGR0070388.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            36865..37557
                     /locus_tag="ACL297_000052"
     CDS             36865..37557
                     /locus_tag="ACL297_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGR0070389.1"
                     /translation="MVKVRLYLIRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(37660..39296)
                     /locus_tag="ACL297_000053"
                     /pseudo
     CDS             complement(37660..39296)
                     /locus_tag="ACL297_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(39286..40376)
                     /locus_tag="ACL297_000054"
                     /pseudo
     CDS             complement(39286..40376)
                     /locus_tag="ACL297_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(40389..41495)
                     /locus_tag="ACL297_000055"
                     /pseudo
     CDS             complement(40389..41495)
                     /locus_tag="ACL297_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(41727..41951)
                     /locus_tag="ACL297_000056"
                     /pseudo
     CDS             complement(41727..41951)
                     /locus_tag="ACL297_000056"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(41999..42775)
                     /locus_tag="ACL297_000057"
     CDS             complement(41999..42775)
                     /locus_tag="ACL297_000057"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGR0070390.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            42897..43643
                     /locus_tag="ACL297_000058"
     CDS             42897..43643
                     /locus_tag="ACL297_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGR0070391.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            43656..44636
                     /locus_tag="ACL297_000059"
     CDS             43656..44636
                     /locus_tag="ACL297_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGR0070392.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            44831..45151
                     /locus_tag="ACL297_000060"
     CDS             44831..45151
                     /locus_tag="ACL297_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGR0070393.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            45197..45505
                     /locus_tag="ACL297_000061"
     CDS             45197..45505
                     /locus_tag="ACL297_000061"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0070394.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            45522..46820
                     /locus_tag="ACL297_000062"
     CDS             45522..46820
                     /locus_tag="ACL297_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGR0070395.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSWYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            46971..49409
                     /locus_tag="ACL297_000063"
     CDS             46971..49409
                     /locus_tag="ACL297_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGR0070396.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            49428..50096
                     /locus_tag="ACL297_000064"
     CDS             49428..50096
                     /locus_tag="ACL297_000064"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGR0070397.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            50114..51202
                     /locus_tag="ACL297_000065"
     CDS             50114..51202
                     /locus_tag="ACL297_000065"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HGR0070398.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            51657..54158
                     /gene="leuS"
                     /locus_tag="ACL297_000066"
     CDS             51657..54158
                     /gene="leuS"
                     /locus_tag="ACL297_000066"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGR0070399.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            54352..55047
                     /locus_tag="ACL297_000067"
     CDS             54352..55047
                     /locus_tag="ACL297_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGR0070400.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            55059..55475
                     /locus_tag="ACL297_000068"
     CDS             55059..55475
                     /locus_tag="ACL297_000068"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070401.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(56290..56382)
                     /locus_tag="ACL297_000069"
     CDS             complement(56290..56382)
                     /locus_tag="ACL297_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HGR0070402.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            56516..57514
                     /gene="ruvB"
                     /locus_tag="ACL297_000070"
     CDS             56516..57514
                     /gene="ruvB"
                     /locus_tag="ACL297_000070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGR0070403.1"
                     /translation="MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEA
                     AKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELE
                     PGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPR
                     IANRLLKRVRDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGG
                     PVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            57495..58067
                     /locus_tag="ACL297_000071"
     CDS             57495..58067
                     /locus_tag="ACL297_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HGR0070404.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            58298..59056
                     /locus_tag="ACL297_000072"
     CDS             58298..59056
                     /locus_tag="ACL297_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGR0070405.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            59065..59868
                     /locus_tag="ACL297_000073"
     CDS             59065..59868
                     /locus_tag="ACL297_000073"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGR0070406.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            59890..61149
                     /gene="rseP"
                     /locus_tag="ACL297_000074"
     CDS             59890..61149
                     /gene="rseP"
                     /locus_tag="ACL297_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGR0070407.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            61162..63015
                     /locus_tag="ACL297_000075"
     CDS             61162..63015
                     /locus_tag="ACL297_000075"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGR0070408.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            63115..64494
                     /gene="bglA"
                     /locus_tag="ACL297_000076"
     CDS             63115..64494
                     /gene="bglA"
                     /locus_tag="ACL297_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGR0070409.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            64686..66494
                     /gene="glmS"
                     /locus_tag="ACL297_000077"
     CDS             64686..66494
                     /gene="glmS"
                     /locus_tag="ACL297_000077"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGR0070410.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDRELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            66613..67662
                     /locus_tag="ACL297_000078"
     CDS             66613..67662
                     /locus_tag="ACL297_000078"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGR0070411.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(67762..71559)
                     /gene="spuA"
                     /locus_tag="ACL297_000079"
     CDS             complement(67762..71559)
                     /gene="spuA"
                     /locus_tag="ACL297_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HGR0070412.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDTHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVKIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEASV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            71976..72389
                     /gene="rpsL"
                     /locus_tag="ACL297_000080"
     CDS             71976..72389
                     /gene="rpsL"
                     /locus_tag="ACL297_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGR0070413.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            72409..72879
                     /gene="rpsG"
                     /locus_tag="ACL297_000081"
     CDS             72409..72879
                     /gene="rpsG"
                     /locus_tag="ACL297_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGR0070414.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            73303..75384
                     /gene="fusA"
                     /locus_tag="ACL297_000082"
     CDS             73303..75384
                     /gene="fusA"
                     /locus_tag="ACL297_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGR0070415.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            75488..79879
                     /locus_tag="ACL297_000083"
     CDS             75488..79879
                     /locus_tag="ACL297_000083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGR0070416.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            79981..80244
                     /locus_tag="ACL297_000084"
     CDS             79981..80244
                     /locus_tag="ACL297_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HGR0070417.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            80237..80515
                     /locus_tag="ACL297_000085"
     CDS             80237..80515
                     /locus_tag="ACL297_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HGR0070418.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            80536..80688
                     /locus_tag="ACL297_000086"
     CDS             80536..80688
                     /locus_tag="ACL297_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070419.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            80710..81951
                     /locus_tag="ACL297_000087"
     CDS             80710..81951
                     /locus_tag="ACL297_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGR0070420.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            81961..82191
                     /locus_tag="ACL297_000088"
     CDS             81961..82191
                     /locus_tag="ACL297_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGR0070421.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            82549..83271
                     /locus_tag="ACL297_000089"
     CDS             82549..83271
                     /locus_tag="ACL297_000089"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGR0070422.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            83488..84822
                     /gene="pepC"
                     /locus_tag="ACL297_000090"
     CDS             83488..84822
                     /gene="pepC"
                     /locus_tag="ACL297_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HGR0070423.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(84878..85789)
                     /locus_tag="ACL297_000091"
     CDS             complement(84878..85789)
                     /locus_tag="ACL297_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGR0070424.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(85813..86616)
                     /locus_tag="ACL297_000092"
     CDS             complement(85813..86616)
                     /locus_tag="ACL297_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGR0070425.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(86644..87633)
                     /locus_tag="ACL297_000093"
     CDS             complement(86644..87633)
                     /locus_tag="ACL297_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0070426.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(87866..88885)
                     /gene="adhP"
                     /locus_tag="ACL297_000094"
     CDS             complement(87866..88885)
                     /gene="adhP"
                     /locus_tag="ACL297_000094"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGR0070427.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWAVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(89237..90049)
                     /locus_tag="ACL297_000095"
     CDS             complement(89237..90049)
                     /locus_tag="ACL297_000095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGR0070428.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            90239..91657
                     /locus_tag="ACL297_000096"
     CDS             90239..91657
                     /locus_tag="ACL297_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGR0070429.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            91710..92417
                     /locus_tag="ACL297_000097"
     CDS             91710..92417
                     /locus_tag="ACL297_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGR0070430.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            92512..93486
                     /gene="folP"
                     /locus_tag="ACL297_000098"
     CDS             92512..93486
                     /gene="folP"
                     /locus_tag="ACL297_000098"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGR0070431.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALATGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            93488..94810
                     /locus_tag="ACL297_000099"
     CDS             93488..94810
                     /locus_tag="ACL297_000099"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGR0070432.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            94791..95345
                     /gene="folE"
                     /locus_tag="ACL297_000100"
     CDS             94791..95345
                     /gene="folE"
                     /locus_tag="ACL297_000100"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGR0070433.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            95388..96200
                     /gene="folK"
                     /locus_tag="ACL297_000101"
     CDS             95388..96200
                     /gene="folK"
                     /locus_tag="ACL297_000101"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGR0070434.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(96245..96562)
                     /locus_tag="ACL297_000102"
     CDS             complement(96245..96562)
                     /locus_tag="ACL297_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HGR0070435.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            96919..97365
                     /gene="rplM"
                     /locus_tag="ACL297_000103"
     CDS             96919..97365
                     /gene="rplM"
                     /locus_tag="ACL297_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGR0070436.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            97384..97776
                     /gene="rpsI"
                     /locus_tag="ACL297_000104"
     CDS             97384..97776
                     /gene="rpsI"
                     /locus_tag="ACL297_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGR0070437.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(98038..98460)
                     /locus_tag="ACL297_000105"
     CDS             complement(98038..98460)
                     /locus_tag="ACL297_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGR0070438.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            98607..98783
                     /locus_tag="ACL297_000106"
     CDS             98607..98783
                     /locus_tag="ACL297_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070439.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            99185..101398
                     /locus_tag="ACL297_000107"
     CDS             99185..101398
                     /locus_tag="ACL297_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HGR0070440.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(101620..102096)
                     /locus_tag="ACL297_000108"
     CDS             complement(101620..102096)
                     /locus_tag="ACL297_000108"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HGR0070441.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQAQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFSKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            102319..105522
                     /locus_tag="ACL297_000109"
     CDS             102319..105522
                     /locus_tag="ACL297_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HGR0070442.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKGG
                     AITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYYWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKGINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            105875..106251
                     /gene="tnpA"
                     /locus_tag="ACL297_000110"
                     /pseudo
     CDS             105875..106251
                     /gene="tnpA"
                     /locus_tag="ACL297_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            106433..106495
                     /locus_tag="ACL297_000111"
                     /pseudo
     CDS             106433..106495
                     /locus_tag="ACL297_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(106639..107268)
                     /locus_tag="ACL297_000112"
     CDS             complement(106639..107268)
                     /locus_tag="ACL297_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGR0070443.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(107278..108279)
                     /locus_tag="ACL297_000113"
     CDS             complement(107278..108279)
                     /locus_tag="ACL297_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGR0070444.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(108310..108951)
                     /locus_tag="ACL297_000114"
     CDS             complement(108310..108951)
                     /locus_tag="ACL297_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGR0070445.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(108970..109785)
                     /locus_tag="ACL297_000115"
     CDS             complement(108970..109785)
                     /locus_tag="ACL297_000115"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGR0070446.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            110056..110490
                     /locus_tag="ACL297_000116"
     CDS             110056..110490
                     /locus_tag="ACL297_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGR0070447.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQESVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            110502..111692
                     /locus_tag="ACL297_000117"
     CDS             110502..111692
                     /locus_tag="ACL297_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HGR0070448.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            111703..112194
                     /locus_tag="ACL297_000118"
     CDS             111703..112194
                     /locus_tag="ACL297_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0070449.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            112209..112988
                     /locus_tag="ACL297_000119"
     CDS             112209..112988
                     /locus_tag="ACL297_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGR0070450.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            112975..113793
                     /locus_tag="ACL297_000120"
     CDS             112975..113793
                     /locus_tag="ACL297_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGR0070451.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGENQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            113793..114086
                     /gene="yajC"
                     /locus_tag="ACL297_000121"
     CDS             113793..114086
                     /gene="yajC"
                     /locus_tag="ACL297_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGR0070452.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            114108..116009
                     /locus_tag="ACL297_000122"
     CDS             114108..116009
                     /locus_tag="ACL297_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HGR0070453.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFMSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            116069..117070
                     /locus_tag="ACL297_000123"
     CDS             116069..117070
                     /locus_tag="ACL297_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGR0070454.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(117180..117515)
                     /locus_tag="ACL297_000124"
     CDS             complement(117180..117515)
                     /locus_tag="ACL297_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070455.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(117526..117768)
                     /locus_tag="ACL297_000125"
     CDS             complement(117526..117768)
                     /locus_tag="ACL297_000125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070456.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(117784..117978)
                     /locus_tag="ACL297_000126"
     CDS             complement(117784..117978)
                     /locus_tag="ACL297_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0070457.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            118144..119094
                     /gene="rsmH"
                     /locus_tag="ACL297_000127"
     CDS             118144..119094
                     /gene="rsmH"
                     /locus_tag="ACL297_000127"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGR0070458.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            119106..119423
                     /gene="ftsL"
                     /locus_tag="ACL297_000128"
     CDS             119106..119423
                     /gene="ftsL"
                     /locus_tag="ACL297_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGR0070459.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            119427..121679
                     /gene="pbp2X"
                     /locus_tag="ACL297_000129"
                     /gene_synonym="pbpX"
     CDS             119427..121679
                     /gene="pbp2X"
                     /locus_tag="ACL297_000129"
                     /gene_synonym="pbpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HGR0070460.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            121681..122661
                     /gene="mraY"
                     /locus_tag="ACL297_000130"
     CDS             121681..122661
                     /gene="mraY"
                     /locus_tag="ACL297_000130"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGR0070461.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHK
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            123225..125330
                     /locus_tag="ACL297_000131"
     CDS             123225..125330
                     /locus_tag="ACL297_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGR0070462.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(125626..126108)
                     /locus_tag="ACL297_000132"
     CDS             complement(125626..126108)
                     /locus_tag="ACL297_000132"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGR0070463.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(126203..127687)
                     /locus_tag="ACL297_000133"
     CDS             complement(126203..127687)
                     /locus_tag="ACL297_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGR0070464.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDATRAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            127840..129450
                     /locus_tag="ACL297_000134"
     CDS             127840..129450
                     /locus_tag="ACL297_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGR0070465.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(129594..130990)
                     /locus_tag="ACL297_000135"
                     /pseudo
     CDS             complement(129594..130990)
                     /locus_tag="ACL297_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            131168..132613
                     /gene="cps4A"
                     /locus_tag="ACL297_000136"
     CDS             131168..132613
                     /gene="cps4A"
                     /locus_tag="ACL297_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HGR0070466.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHRKFLFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGTDRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            132615..133346
                     /gene="cps4B"
                     /locus_tag="ACL297_000137"
     CDS             132615..133346
                     /gene="cps4B"
                     /locus_tag="ACL297_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HGR0070467.1"
                     /translation="MIDIHSHIVFDVDDGPKSIEESKALLREAYNQGVRMIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYREIHAGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVLKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKEFFVDNPRKIIMDQLI"
     gene            133355..134047
                     /gene="cpsC"
                     /locus_tag="ACL297_000138"
     CDS             133355..134047
                     /gene="cpsC"
                     /locus_tag="ACL297_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HGR0070468.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            134057..134740
                     /gene="cpsD"
                     /locus_tag="ACL297_000139"
     CDS             134057..134740
                     /gene="cpsD"
                     /locus_tag="ACL297_000139"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HGR0070469.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARTGYKTLLIDGDTRNSVISGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVIQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTAIGEVNKRDVQKAKQQLEQTEKLFLGVVLNKLDISVDKYGVY
                     GSYGNYGKK"
     gene            134756..136123
                     /locus_tag="ACL297_000140"
     CDS             134756..136123
                     /locus_tag="ACL297_000140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGR0070470.1"
                     /translation="MDEKGLKIFLAVIQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMG
                     IDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLIL
                     FAIASLVLVPLIRKDGGSAIFAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQLMDQNTM
                     QGGMFKIDNDPRVTKIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPE
                     QKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            136129..136872
                     /locus_tag="ACL297_000141"
     CDS             136129..136872
                     /locus_tag="ACL297_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HGR0070471.1"
                     /translation="MNERMQILGVTIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCHEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEILQDMLKVFKQRYPNLNVVGYRNGYFSPEDEKKIQEDIKGGKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQKLNLEWLFRVVN
                     EPKRLFKRYFIGNVTFIKRVLDEKRKSKK"
     gene            136847..137932
                     /locus_tag="ACL297_000142"
     CDS             136847..137932
                     /locus_tag="ACL297_000142"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGR0070472.1"
                     /translation="MKSENQKNNILHISRTMDIGGAERIVYQLSSDLKDEFDSVHVAS
                     TGGLWESKLLSKGVQHHKILDVDSKNPLTMVKILSSFYRIIKNNDITIVHTHHRMAAF
                     YIRLLKIINPKLIHVYTAHNVFKNKLPLYKFSLKKAYCVAVSKAVNDNLKHDVGISDS
                     TVIYNGVVFEKTDKLVGEIVNYNGIKLGCIARLSEQKGLTYLIDAMSLVKHRNITLFI
                     VGDGELRDELENKVKQLNLQKSVIFLGYRKDIVECINSFDYLVSSSLYEGFGLNAIEA
                     FMKGKTMVSSDIPGINEVVNNKNGILVPVKDDVALARAIEKLATDKKLREKLAYQAKK
                     DYETKFNYSLFLDNYRRLYRKLMGESK"
     gene            137929..139017
                     /gene="wecB"
                     /locus_tag="ACL297_000143"
     CDS             137929..139017
                     /gene="wecB"
                     /locus_tag="ACL297_000143"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGR0070473.1"
                     /translation="MKKVMLVFGTRPEAIKMCPLVNELKQNDSIKTLVCVTGQHKEML
                     EQVLEVFKVVPDYDLGIMKANQTLFTITTSILDKIQAVLEKEKPDLVLVHGDTTTTFA
                     TALAAFYMGIKVGHVEAGLRTYNLQSPFPEEFNRQTTSIIADYHFAPTEVAKENLLRE
                     DRKNIFVTGNTVIDALKTTVQDNYHHPILEWAKGSKLIMLTAHRRENLGKPMENMFNA
                     VNRILNEFEDVKVVYPIHKNPKVRELASKVFGDNERMKIIEPLEVIDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVVAGTLKLVGTEEENIYSNFKLLLEDET
                     EYNKMSKASNPYGDGMTCKRIVDIILEV"
     gene            139134..140198
                     /locus_tag="ACL297_000144"
     CDS             139134..140198
                     /locus_tag="ACL297_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGR0070474.1"
                     /translation="MATLFALLSTLVKLFVTFDYIPKVLLQHKVTLIDILVVLFLFFQ
                     AFAAFQSQTLYLNYVGGQFFLLGLYSFLKHFLLLDCKTTIKSLFLTFILFLCVQVITQ
                     LLFPVGFDSLHPTGDNRLYFLGRKNIATPYIIVGLGGFYLLNKKMNEFISLKEIIFLG
                     LFGILSFLTQSSTAIICYGLFIFIRLLGLKENIGKLYSLVSMAVYVCFSLSIIFSQST
                     ILSTFTAIFSKNATFSGRINIWQLAIRIFEENFWFGRGLNVNFNAWTNGIIVNSAHNT
                     LLDILARTGIFPGVLFIVLLMNLFLGKYRIESKTLLAMLASFMIYITMETSSMSILLL
                     IIVLCVYWPRGEEKLHEQVT"
     gene            140182..141051
                     /locus_tag="ACL297_000145"
     CDS             140182..141051
                     /locus_tag="ACL297_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGR0070475.1"
                     /translation="MSKLLNTIYYRLFRLYKKIYLQVFSKKTVDILGEKANLENELIV
                     SFTTIPSRLNYLPSMIKSIFNQTIIPNRFIMYVYKDEFEGINLESILELEIKNGLEIV
                     YVDENLRSHKKYFYAMKDNPNSIVVLVDDDIIYPRNTIKKLIASYRIYPQCVSAMRCH
                     RIKLFSDGSLYPYNQWEYEISGATIPSYFNFFTSGGGTLFPPCTRNEDLFNKKNIREL
                     SFLADDVWLNFLVVKNGIKTVKATRYKGTPLTIDDNPEESLVYLNTVYDNNNDKCIRN
                     MVEYYQINFTEDK"
     gene            141051..142475
                     /locus_tag="ACL297_000146"
     CDS             141051..142475
                     /locus_tag="ACL297_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGR0070476.1"
                     /translation="MNVEKNLKRGIFYTAIGKYSNVVIQLLVTAILSRILSPVEYGIV
                     AVVNVFLLFFQMLADSGIGPAIVQNKELNKSDLISIFSLTIYSGIVLSCIFALLGYPM
                     GVLYGETIYVSLFPLLGLCVLFYTITIVPQAILMKTMNFKIVNFLTIFSNIASGLVGV
                     ILAVSHFGVYSLIFSNIVKAMVLFIALYSKAKINFYWKVSKSSIGKIFEFSKFQFLFN
                     FWNYFARNLDNLLIGRLISPSDLGYYDKAYQLSLYPNQILSQVVTPALHPIMSNFQDN
                     ISKIGEVYLQISRIFVIVGIPISAYLYFNAQYVVTFMFGDNWSQSVPVFQILAATIWL
                     QMANSPAGAFYQATNQTKLLFRIGLLTSFINILAIVIGVMLQSIQCVAYMLLISFSLS
                     LVINTLYLTKKVLNISAQKYIKPILINLTIISPYIVFNLFISDFVNDLILHLSVQFVI
                     LFLIWGIGMYISGEYRRVFAVIRK"
     gene            142488..143690
                     /locus_tag="ACL297_000147"
     CDS             142488..143690
                     /locus_tag="ACL297_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGR0070477.1"
                     /translation="MKILFVNMFYNEAGGAQKSTKFLAEQLVKEGHEVFAYSDDAIDS
                     DIDEVINRVKIFRRRTPLFSLHYIFSAKKNPAKHFFYKIFETYNVFAKRKFINIIEEV
                     QPDIVHFNTISGMSLSIVKEAKKRNIRTVWTLRDYWLEYPWGEKDISLIKLLNRIYRP
                     FVKKSLRHLDIVTAPSAFTLNNFINKGFINPSSQKCMVIENAVIFSKGKLENIIKQKQ
                     ETKRNPEKTNFLFVGSILEIKGVFNLVKAFKKLTSPEISLKIVGKGKDLEKLQKEIES
                     DPRIQYLGFQDTETLYKTYQNSDVLVVPSAWDEPFGRVVIEGNANGLPVITSDRGGLP
                     EIVQTVGGGEIFTDESGNTLYSLLKKFVEMENYNVYYQCILENIDIYSIEKQSEKFTN
                     LYLDKEIE"
     gene            143724..144956
                     /locus_tag="ACL297_000148"
     CDS             143724..144956
                     /locus_tag="ACL297_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGR0070478.1"
                     /translation="MKIAVAGTGYVGLSIAVLLAQHHEIKAVDIVAEKVELINQKKSP
                     IQDDYIEKYLVEKDLNLEATMDAKYAYEDAEFVVVAAPTNYDSKKNFFDTSAVEAVIQ
                     QVKKINPKAVIIIKSTIPVGFTESVQKKYQYKNILFSPEFLRESKALYDNLYPSRIIV
                     GTDLEDTYLKDKANIFASLLKEGAIKEDIDTLFMGVTEAEAVKLFANTYLALRVSYFN
                     ELDTYAEVKGLDAKAIIDGIGLDPRIGNHYNNPSFGYGGYCLPKDSKQLLANYFDVPQ
                     NMMTATVESNRTRKDFIAQRILEKAGVSETDSLDAFKNIQDIVIGIYRLTMKSNSDNF
                     RHSSIQGVMKRLKAKGVTVIIYEPTLKDGETFFGNKVVNNLDKFKEASNVIVANRFEP
                     SLEDVSNKVYSRDIFKRD"
BASE COUNT        44051 a        25870 c        32122 g        43181 t
ORIGIN      
        1 atgccatctg tcattgcaaa ttatcttgtt tatgtagttt tagctttata tggctccttc
       61 ttgttcaagg atagattgat ccaacaatgg aaggagatta gaaagactaa aagaaaattc
      121 ttctttggcg tcttaacagg atggctcttt ctcattctga tgactgttgt ctttgaattt
      181 gtatcagaga tgttgaagca gtttgtgaga ctagatggac aaggtctaaa tcagtctaat
      241 attcaaagta cctttcaaga acaaccacta ctgatagctg tttttgcttg tgtcattgga
      301 cctctggtag aagaattatt tttccgtcag gtcttattgc attacttgca ggaacggttg
      361 ccaggtttac taagcattat tctggtagga cttgtttttg ctctgactca tatgcacagt
      421 ttggccctat cagagtggat tggtgcagtt ggttacttag gtggaggcct tgccttttct
      481 attatttatg tgaaagaaaa agagaatatc tactatcccc tacttgttca catgttaagc
      541 aacagcctct ccttaatcat tttagctatc agtatagtaa aatgaaatga gaacaggaca
      601 aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatatactgt
      661 aatatgtgat gaaaatgcca gtaatgatac cgagaaaaaa gctgagaaac ttttcccagc
      721 tttatttgtt atagtcaaag agaatgactt gttcctgtgc atctacatga gcatggaccc
      781 caaagggtac aattgctctt ggagttgcgt ggccgacatt cagattatag acaatcggga
      841 tattgctgtc aatgatatcc aatagtgcct ctttatagtc gtcatggaaa gtttcatcca
      901 taggttttcc gaccaagagt ccattgatga ccgcgaatat gccagtgtcc tttaaagtta
      961 gcaacatctt tttgaagtct tctggcttag gcttttcttc gcttgtttcg agcaagagga
     1021 ttttcccttc ccagtctgac aagtcaggga aaagtttgta tttttggcag agttccgtgc
     1081 tatctgcgta tcgagagttg tcaaagatat cgtagaggga ttcgaggcaa ccaccgagga
     1141 ttttcccctc gaactgggca cttccttgca acaagtcaaa acctgtattt gtatgactga
     1201 cacgaggtgt tcccagggcc gtgggactaa aatcagttcg ttcctcatac caaacgtcac
     1261 tagggcggat ttctgaaatt cttcccgtct caatcaattc tttaaagtag tgaaggctat
     1321 aggctagcat ttctttgtct aattcacaaa tgtctgctaa aaaggattga ccataaaaag
     1381 tcttgattcc taatttatgc aacatgaggt ggttcatggt tgtatccgag aagccaagaa
     1441 aaattttttg tttgataacc ttttggagtt ggtcattttc aaaaagataa ggtagcaagc
     1501 gataggtatc gtctccaccg atggcacata ggatcatgtc gatgctatca tcagaaaagg
     1561 catgaatcaa atcctctgca cgagcttcag gatggtcctt gataaagtct aatcctttta
     1621 acgaatgggg caaaaagatg ggattgagtc ccagatcctt gagacgttgg atacccaagt
     1681 ccacttcgtg tttgacaaaa tcctctccga taatgccact agataaacta acaataccaa
     1741 tagtagaaac catatctcat cctcctagaa atagattgag cctattttat cacaaaatat
     1801 aagagaaagc tatgtgggat ttcagaagaa tacttttaat actcttcgaa aatctcttca
     1861 aaccacgtca gttttatttg caacctcaaa gctgtgcttt gagcaacctg cagctagctt
     1921 cctagtttgc tctttgattt tcattgagta taactcaaaa aagtaagtga aaaagcaacc
     1981 gcacctgcag ttgcttttat cgttttctta ttcttgatag cgtgttggtc ctgcacttgc
     2041 actacggata ttcaagcgtt tcttgagata gaagcgaagg gctaggaggg ctactccaat
     2101 aatagctagt ggcaatggat ccagtactgg gttaaggcta gctggtagga agcttgttgc
     2161 aaagaagaca agcaaccaaa ggaacataga agttaggata actagtacag atttccagaa
     2221 aggtggacgt tgactgcgat ccatatctgg tccatagtat tggtaaacaa agtagtacat
     2281 caagtagaag gcaaatccac caaccagtcc aactaataga agagtaatca atccatagcc
     2341 gaaagcttga tctgccgcaa agaaggttgt gagggcgctg acaagggcaa agaggctagt
     2401 gatgaaaaga gctgagtcca taatcatcag ttttggatca tcattttctt ttggatgctc
     2461 tttttcgtac tgctctttga cagtgaagct atgagcccaa tgagttggtg cgccgtatag
     2521 ggaacgggca gttgtacctt tagattgctc ctcaaggatt tggggaatga cttcctcaaa
     2581 aatagtctgg atttcagcgt ctgttttccc atctttgatg aattgttggg tagcaatgtg
     2641 gacaaactct tggtttttct tagttaattt ttgtagatca atttgagaca taggaactcc
     2701 tcttagaacc attttttatg gatgagatag agagtgagcg agacactcat agcaaaggcg
     2761 ataaagacga ttaaccagaa ggcatttggc tctccgttta ggggaatttc attatcctta
     2821 aagttcatcc cgtaggcaga aaagaccatg gttgggatgg acatgacgat ggtcacaagg
     2881 gccaaggttt tcatgatgtt gttctggttg ttagaaatga tagaggcaaa ggtctctgtc
     2941 atagaatgca agacgtttcc ataaatatct gccatctcga tggcctgttg ggtttcaatc
     3001 agggtgtctt caagcaggtc ttcgtcctca aggtatttct tgatattgct ggttgaactg
     3061 gtcaatttct taatcacgcg ctcatttgtt ttgagggagg ccttgaaata gacgatagtt
     3121 ttttccaatt ccatgagctc aatcaattct tcattacgag ttgattgatg cagttgactt
     3181 tcgatttgtt cactcttgcg gtcgattgaa cgaagggctg ttaggtaaag ctctgcattg
     3241 cgataaagaa tttgaaagat aaaacgtgaa cgcatgaagg tatagaaatt acgcaatcga
     3301 cggttgataa agacatcaag gacaggtagt ggttccaaac acgtagtgat aatggtttcc
     3361 tcagtgatga taataccaag cgggatggtt acgtagtagg tgcggttatt tctttcctcc
     3421 gtgaccggca cgtctacgat aatcagggta tactcgtctt caatggtaat acgagacatt
     3481 tcttccgcat cgagcggtgc tcgaaggtcg gcaatatcaa tatcgaaggt gttggcgatt
     3541 tcgagtgatt cattttgagt cggattgacg agattgatcc aagtacccgg ttcaagcgta
     3601 tcgatctctt taaattcagt tgttgtagag agaaaaactt gtttcatatc ccttatcctt
     3661 tctcattttt cagatttttt cacactgtac tattatacta caaaatcggc cttttgggta
     3721 atagaatctc actttttttg gtataatggt aagcaataat ggactagaaa gaacaaagat
     3781 gcaagataaa attgtcattc acggggcgcg tgcccataat ttaaaaaata ttgatgtgga
     3841 gattccgcga gacaagttgg ttgtcgtgac tggcttgtca ggttcaggga agtccagtct
     3901 ggcttttgat actctctatg cggagggaca acgtcgctat gtagagagtt tgtctgccta
     3961 cgctcgtcag ttcttgggaa atatggaaaa accagatgta gatgccattg atggtctcag
     4021 cccagctatt tccatcgacc agaaaacgac tagtaaaaat cctcgctcga cggtgggaac
     4081 gacgactgaa atcaatgact atctgcgtct cctctacgca cgtgtgggga cgccttactg
     4141 tatcaacgga catggagcta tcaatgcttc ttctgtggag caaatcgttg ataaggtttt
     4201 ggagttacct gaacgccagc gcttgcagat cttggctcct gtcatccgca agaaaaaagg
     4261 ccaacataag agtgttatcg agaaggttca gaaagacggg tatgttcgtg ttcgtgtgga
     4321 tggggaagtc tatgatgtga ccgaagtgcc agagttgtct aagagcaagc aacacaatat
     4381 cgatgtcgtg gttgaccgta ttgttatcaa ggagggcatt cgtagccgtc tctttgattc
     4441 cattgaagct gcccttcgta tcgcagaagg ttatgtgatt atcgacacca tggacgactc
     4501 tgagttgttg ttctctgagc attatgcctg tccagtttgt ggatttactg tcccagagtt
     4561 agaacctcgt ctcttctctt tcaatgctcc ctttggctct tgtagtgagt gtgacggctt
     4621 ggggattaag ctggaggtgg atactgattt ggtagtgcca gatgccagca aaaccttaca
     4681 tgagggagcg ctggcacctt ggaatcctat ctcatccaac tactatccaa acatgttaga
     4741 gcaggccatg aaagtctttg gagtggctat ggataagcct tttgaggacc tgtcagaaga
     4801 agataagaac ttgattctct acggctcaga tggtaaggaa ttccatttcc attatgaaaa
     4861 tgaatttggt ggtgtgcgcg atatcgacat tccgtttgag ggagttatca ataatatcaa
     4921 acgtcgttac catgaaacca atagcgatta cactcgcact cagatgcgtc tctacatgaa
     4981 tgagctgacc tgcggaacct gtcaaggcta tcgtctcaat gaccaggcct tgtctgtccg
     5041 tgtgggcggc cagcaagggc cacatattgg agaaatctca gacctgtcta tcgctgacca
     5101 cttggacttg gtgagccagt taactttgtc tgaaaatgaa gccatcattg ctcgtcccat
     5161 tctcaaggaa atcaaggatc gtttgacctt ccttaataat gtgggtctta actatctaac
     5221 cctgtcacgt tcagcaggaa ccctttcagg tggggaaagt cagcgcattc gtttggcaac
     5281 ccagattggt tccaacctat caggtgtcct ctatatccta gacgagccgt caatcggtct
     5341 tcaccagagg gacaatgacc gcctgattgc cagtctgaaa aagatgcgtg acttgggcaa
     5401 tactcttatc gtggtggaac acgacgaaga taccatgcgt gaagctgatt atctgattga
     5461 cgttggtcct ggtgccggtg tttttggtgg tgagattgtt gcagcaggta cacctaagca
     5521 agtggctcgc aacagcaagt ctatcacagg tcagtacttg tcaggcaaac gtgtcatccc
     5581 agtaccagaa gagcgccgtg tcggaaatgg tcgttttatt gaagtgacag gagcgcgtga
     5641 gaacaacttg caaaatgtca cggctcgctt tccactagga aaatttatcg cagtgacagg
     5701 tgtgtcgggt tcagggaaat cgactctaat caacagcatt ctcaaaaaag ccattgccca
     5761 gaagctcaac cgtaattcag acaaacctgg taagtttaag actattacag ggattgagca
     5821 tgtcgaccgt ttgattgata ttgaccagag ccctatcgga cggacgccga ggtctaaccc
     5881 agctacctat acaggagttt ttgacgatat acgtgacctt tttgctcaga caaatgaagc
     5941 caagattcga ggctacaaga aggggcgctt cagtttcaac gtcaagggag gtcgctgtga
     6001 agcttgctca ggtgacggga ttatcaagat tgagatgcac ttcttgccag atgtttatgt
     6061 ggcttgtgaa gtttgccacg gaacccgcta taacagtgaa accctagaag ttcactacaa
     6121 ggaaaagaat atttcgcagg tcttggatat gacggtcaac gatgcggtag aatttttcca
     6181 acacattccg aaaattcaac gcaaactcca gaccatcaag gatgtaggtc taggctatgt
     6241 gaccttggga cagccagcta ccaccctttc tgggggagaa gcccaacgta tgaagttagc
     6301 tagcgaactc cacaaacgct cgacaggaaa atctttctac attctggatg agccgacgac
     6361 agggcttcat acagaggaca ttgctcgcct gctcaaggtt ttagctcgct ttgtagacgc
     6421 cggcaataca gtcctcgtca tcgagcacaa tctggatgtt atcaagacgg cagaccatat
     6481 cattgacttg ggacctgagg gcggtgtcgg tggtggaacc atcatcgtaa caggaactcc
     6541 agaagaagta gctgccaacg aagccagcta tacaggacac tatttgaaag gaaagttaca
     6601 tcatgaataa acgcgtacaa gcatttctag ctaaaatgca agaaaaagaa ctagatggta
     6661 tcatcatcaa caatcttaaa aacgtctatt atttgactgg tttttggggc tcaaacggaa
     6721 cagtctttat cagccgtgac cgtcaggtct tagtgacaga ctctcgctat atcatcgcag
     6781 ctaagcaaga aaccagtggt tttgagattg tggctgatcg tgatgaattg gctgtcattg
     6841 caggaattgt taaggacatg aacttgactc gtatcggttt tgaagatgag atttcagtgt
     6901 cttattacca ccgtatgcag gcagcttttg caggtttgaa cttgcttcca caaactcagt
     6961 ttgtggaagg tcttcgtatg attaaggatg aggcagagat tgcagcgatt cgcaaggctt
     7021 gttctatctc agaccaagct ttccgcgatg cgcttgactt tatcaaacca ggaaaaactg
     7081 aaattgagat tgccaacttc cttgatttcc gcatgcgtga gttgggagca tctggcttat
     7141 cttttgatac gatcctagct agcggtatca attcttctaa accccatgcc catccaatgc
     7201 acaaaccagt ggagttggga gaagccatta ccatggactt cggctgtctc tatgaccact
     7261 atgtcagtga tatgacccgg actatctatc tagggcatgt tagcgatgag caggcagaga
     7321 tttacaatac ggttctaaaa gctaaccaag ccttgattga ccaagctaag gcaggcttag
     7381 gtttccgtga ctttgacaaa atccctcgtg atattatcat tgaggcaggt tatggtgact
     7441 actttactca cggcattggc cacggtattg gtctggatat ccatgaggaa ccctacttta
     7501 gtcagacttc tacagaaact attaagacag gtatggcctt gaccgatgaa ccaggtatct
     7561 atatcgaagg caaatatggc gttcgtatcg aggatgatat cctgattaca gagacaggtt
     7621 gtgaattatt gaccctagct ccaaaagagt tgatagtcat ttagttgttt gtcaagaaaa
     7681 aagtacgaaa atgacgaaaa aagtcaaaaa aaattaaaaa taggtcgcaa gtcggatgtt
     7741 ttttatggta taatagacta aactgatagt aacatgtagc gaaaggggta ggtacatgat
     7801 taaaatttat acagtctcaa gttgtactag ttgtaaaaaa gcaaaaacct ggctcaatgc
     7861 ccaccagtta agttataaag aacaaaacct tggtaaagaa ggaattacga gagaagaatt
     7921 actggatatt ctaaccaaaa cagataacgg aatagccagc atcgtttcgt ctaaaaatcg
     7981 ctatgccaaa gcccttggag tggatattga agatttgagt gtcaatgaag ttctcaatct
     8041 gattatggaa acaccgagaa ttttaaagag cccaatcctt gtagatgaaa aacgcctgca
     8101 agttggctac aaggaagacg atattcgtgc cttcctacca cgctctgtcc gtaatgtaga
     8161 aaatgcagaa gcacgtttgc gtgcagctct ataaacatca aggctgggag cacttcccag
     8221 tcttattcta ttttaatttc aaaaagaaag aagaaagaaa tgaaaaaaat agttcttgtt
     8281 agtctagctt tcctttttgt cctggttggt tgcggacaga aaaaagaaac tggaccagct
     8341 acaaaaacag aaaaagatac gcttcagtcg gcattgccag ttattgaaaa tgctgagaag
     8401 aatacagttg taactaagac tttggtcttg cccaagtcag atgatggtag ccagcagaca
     8461 caaacaatta cttacaaaga caagactttt ttgagtctag ctatccaaca aaaacgtcca
     8521 gtctctgatg agttgaagac ttatattgac caacatggag tggaggaaac tcaaaaagct
     8581 cttcttgaag cggaggagaa ggataagtct atcattgaag ctcgtaaatt ggcaggtttc
     8641 aaacttgaaa caaaactatt gagcgcaacg gaacttcaaa caacgactag ttttgatttt
     8701 caagttctgg atgtcaagaa ggcttcccag ttggaacatc tgaagaatat tggtttggaa
     8761 aatcttttga aaaatgaacc aagcaaatat atttcagata gattggcaaa tggcgcgaca
     8821 gaacaataga aaatccaaat aaatagactg cctgaattgt agaaggtaag gaattatgct
     8881 ataatggtac tattctaagg aaagagggtg ttagaatggg atttactgaa gaaacagtac
     8941 gttttaaatt ggacgattcc aataaaaaag aaattagcga aactttgaca gatgtttatg
     9001 cttcgttgaa cgataagggt tacaacccaa ttaaccaaat cgtaggttac gtattgagtg
     9061 gagaccctgc ctacgttcct cgttataata atgcacgaaa tcaaatccgt aagtatgagc
     9121 gtgatgaaat cgttgaggaa ttggttcgct actacctcaa aggacaagga gtcgatctat
     9181 aacctatgag aattatggga ttggacgtcg gttcaaaaac ggtaggggtg gcgattagcg
     9241 atccgcttgg ttttacagct caagggcttg aaatcatcca gataaatgaa gaacaaggcc
     9301 aatttggttt tgaccgcgtt aaggaattgg ttgatactta caaggtggaa cgatttgtag
     9361 tgggcttgcc taaaaacatg aacaatacaa gtggaccgcg cgtagaagct agtcaagcct
     9421 acggagcaaa gctagaagag ttttttggtt taccagtaga ctatcaggat gaacgcttga
     9481 caacagtggc tgctgagcgc atgttgattg aacaagcaga tatcagtcgc aataagcgca
     9541 agaaagtcat tgataagtta gcagctcagc tgattttaca aaattattta gatagaaaat
     9601 tttaatataa aggagaggct atgtcacacg atcataacca cgaccacgaa gaacgtgaac
     9661 taatcacact agtagatgag caaggaaatg aaaccttgtt tgaaatcctt ttgacaattg
     9721 atggaaaaga agaatttggt aaaaactatg ttcttctagt accagttaac gcagaagaag
     9781 acgaagacgg acaagttgaa atccaagctt actcattcat tgaaaatgaa gatggaacag
     9841 aaggcgaatt gcaaccaatc ccagaagact cagaagacga atggaacatg attgaagaag
     9901 tcttcaacag ctttatggag gagtgataca gtctgggaga ctgtatcagc ccaactccag
     9961 aaatggggaa gagttggaac gtccagtgaa catttttacc ccaactctta gaaattgaga
    10021 agagttggaa catctagtgg atgtttttag cccatattca cattcatagt agctagttat
    10081 tagctaacca ggtaagaggt cgggacgaaa atcctggcct tgtttttatt agtaaccata
    10141 ctttaatgcg ataattgatt ggacttttgt caaggagatg tgtatgtttg aagtagaaga
    10201 atggctccat agtcggattg gtttgaattt tcgatcaggt ttgggccgaa tgcagcaagc
    10261 ggtggatttg ttaggaaatc ctgagcagtc ttaccctatt atccacgtaa cagggactaa
    10321 tgggaaagga tctaccattg cttttatgag ggaattattt atggggcatg gcaaaaaagt
    10381 tgcgaccttt acctcccctc atatcgtctc tatcaatgac cgaatctgca ttaatgggca
    10441 acctatagca gacgcagact ttatccgttt gactgatcag gtcaaggaga tggagaaaac
    10501 gcttctgcaa actcctgccc agttgtcctt ttttgaattg ctgaccttag ttgcttttct
    10561 ttattttagg gagcaagagg tggatttggt tttattagaa gtgggaattg gtggcttact
    10621 tgacacgacc aatgtggtaa ctggagagct tgctgtcatc acctccattg ggcttgacca
    10681 tcaagaaacc ttgggtgata gtctagaagc aattgcagag cagaaagctg gtattttcaa
    10741 ggctggtaaa aaggcagtga ttgcgaaatt gcctccagaa gctagacttg tctgtcagaa
    10801 aaaagccgaa tctttagctg ttgaccttta tcaggcaggt caagattttt cagtgctgaa
    10861 tggtgatttt tcaagctctt tactaaatct ttcacagctg aacataggct tagaaggagc
    10921 ctatcagcag gagaatgcgg ccttggcgtt gcaaactttt cttcttttta tgggagaaag
    10981 aaaggaagct attgatgaac aggctgtaag aaaggccttg gaacagaccc attgggctgg
    11041 tcgcttggag cgtattcgcc cacagattta cttggatggt gctcataacc tccctgcctt
    11101 gactcgcttg gttgagttta tcaaagaaaa agagcaggaa ggctatcgac ctcaaatcct
    11161 ctttggatcc ttgaaacgta aggattatca agggatgttg ggttatctga ctgaaaattt
    11221 acctcaagtg gaactcaaaa tgaccggctt tgactatcaa gggtctttgg acgagacaga
    11281 tgtaacaggt taccatgtaa ttccctctta ccgagaattt atcagcagtt tcgaagaaag
    11341 ggcagacgct caagacttgc tgttcgttac agggtctctc tattttatct cagaagtacg
    11401 gggctacctg ctggaccgtg agcagataaa ttgaccccct tttttgaact tgttatacta
    11461 ggggaattgc tggttagaga aaatttctct aaattggtag cagaaaggaa attcatcatg
    11521 aaattaaaaa gattcacact ttctcttgct tctctagcaa gttttagtct cttagtagct
    11581 tgttcacaaa gagctcaaca ggttcaacag cctgttgctc agcaggtcca acagcctgct
    11641 caacagaata ccaatactgc aaatgcagga ggtaaccaaa atcaagcggc tccagtacaa
    11701 aaccaacctg ttgctcaacc gaccgatatt gatgggactt atactggtca ggatgacgga
    11761 gaccgtatca ctttagtggt aactggaacg actggtacat ggactgagct cgaatctgac
    11821 ggggatcaga aagtcaaaca ggttacattt gattcagcaa atcaacgcat gattattggc
    11881 gatgatgtca aaatttacac tgtaaacggt aatcaaatcg tcgtagatga tatggataga
    11941 gacccatcgg accaaatcgt tttaactaaa taagactatg caagtagaaa gggctggatt
    12001 tttcagcctt tttactttta cctagaaaat aatcataaat acttgccttc taccgaatac
    12061 ctgcgttata ctagtatcaa ttacttgttt ttagcattca aactatcaag gaggggatat
    12121 gaaatatagg aaatttcaat tattgatgtc caagtatggc tttagtcttt cgattatgct
    12181 acttgaactt tgtcttgttt ttggtctctt tctttattta ggacgcatgg ctcccatttt
    12241 atggattact gtcctcattc tactgagtat catcacaatc atttcgatag tcaaccgtaa
    12301 tacgactcct gagaataagg taacctggtt gttagtagcc tttgtgccag tatttggtcc
    12361 cttgctctat ctgatgtttg gtgaaaggcg attgtccaaa aaagaaatca aacaactgaa
    12421 gaagctaggc tctatgcatt tccaagaagc aaatagccag ctactaaaag agaaattaaa
    12481 agaaagtgac aaggcagctt atggagtcat caagtcctta ttgagtatgg ataccaatgc
    12541 tgacatctat gatcaaactg cctctacatt ttttcctaac ggagaagcta tgtggaaaaa
    12601 gatggtagaa gatcttaaaa aggctgagaa atttattttc ttggaatatt acattataga
    12661 agaaggtttg atgtggaatc gcatactaga tatactagag caaaaggtag ctcagggtgt
    12721 agaggttaag atgctctatg atgatatcgg ctgtatggct actttaacag gagattatgc
    12781 acatcgactt cgtcagctgg gcatcgaggc ccataaattc aataaagtta ttcctcgttt
    12841 gacagtggct tataataaca gagatcatag aaaaatattg attgttgatg gtcagatagc
    12901 ctatactggt ggggtcaatc tggcagatga gtacattaac cacgtcgaga gatttggtta
    12961 ttggaaggat agtggaattc gcttagacgg actagcagta aaagccctga cacgcttatt
    13021 tttgaccact tggtacatta atcgaggaga aattagtgat tttgatcaat atcatttaga
    13081 aaatcattct atcccgagtg acggtttaac cattccatac ggaagtggac ccaagccaat
    13141 ttttcgagcg caggtaggga aaaaagttta tcagagttta atcaatcaag caacagaatc
    13201 ggtctatatt acgacacctt atttgattat agattatgat ttaacagaga caatcaaaaa
    13261 tgcagctatg agaggggtcg atgttcgaat tatcacccct tacataccag ataagaagtt
    13321 cattcagtta gtcacgagag gagcttatcc cgaccttctt tctgctggtg ttcggattta
    13381 tgagtatagt ccaggtttta ttcatagtaa gcagatgttg gtagacgaag attttgcggt
    13441 ggtggggaca atcaatctcg actaccggag cttggtacac cattatgaaa atgcagtctt
    13501 actctataaa actccttcta taagggaaat cgcccgagat tttcgaaata tatttgcaga
    13561 ttctcaggaa gtctatcctc attctatcaa aacgagctgg tatcaaaagc ttgtaaaaga
    13621 aatcgcccag ctattcgccc ctatcttata agaaatctag aacttaggat acaaaccaga
    13681 cctatttttc ttgtctttta ctaatagaaa gaaagaggag gggaattcat gctaactaag
    13741 gaagaagtga atgatatgat tgagttcaaa cagacacatt tataccgctt tcgggaaatt
    13801 gaaacctttg tgaaactatg caaaaaatca ctcaaacagc catcgcaagc tgacaatcct
    13861 aggacttttt gatatactaa gacagataaa ttgaacagga gaaacgatat gagtgtgtat
    13921 ggtagagtag aagaagttca taaggagaat cgtgaacctc tagaatacca aatcgaacaa
    13981 gaatcgcatc atcgtgaatc aagtcgtctt cctttggtga aaattttact atggagtacg
    14041 cttgtaacgg ggataactct aggagtaccg ctattactcg acttaatgag tgcacaagaa
    14101 gtgcaggatt tttatgcagg ttgggggctt catcagacag ggaagattta cagcgactat
    14161 tatggaagtc aaggtttgct ttattatttg ctgacttacg tgagtcaggg cggatttttc
    14221 tttgccatct ttgagtggtt agccttggta gcaggaggat ttttcctttt tagatcagcg
    14281 gacaccttga cagagcaagg agaccaagct ggacatctgg tgactatttt ttacatgcta
    14341 gttacaggtc ttgcttttgg tggaggctat gcgactcttt tagcgcttcc tttcttattc
    14401 gcagccttta gtttagttgc ggcttaccta agcaatccaa gccatgataa gggatttgta
    14461 cggattgggc tagctttggc aggcggattt ttctttgctc ccttatcatc gctcctgttt
    14521 attgctgtag tgagtttagg cttgttggtc tttaaccttg ggcatagacg ctttgcgcat
    14581 gggttttatc agtttcttgc agtggcttta ggtttttcac ttgtctttta tccaactgcc
    14641 tactatagtg ctgcaacagg aagttttggg gatgcgatta gtggtattcg ttatcctatt
    14701 gacagtattc gctttgattt tacttctaaa attttagaga atatgttttt ttatggcttg
    14761 ttgtcccttg gtttgggatt tgtggtttgt atctttttag gtctctttca atccaaaccc
    14821 tttaaactat acgtcatttc ggttcctgca agtcttgtgg taattttagg tttgatttta
    14881 cttttctttt cacaagagcc tctgcacgct tcttatttga tggtcgtctt ccctgttttc
    14941 ctacttttat tggtaaccaa tattaagagt caacagaggg ggcgtagtgc tagaagaagc
    15001 cgaagagaaa cgccagttag cctatggagt cgtttcttca aaggaaatct atatctgcta
    15061 gtttttgggt ttgtctatct tttgtctgtt ccttttttga tgaagtttgt cctttatcca
    15121 gtaccttatc aagaacgtaa tcgtcttgct gatttggtaa aagaggagac aaatacggaa
    15181 gatgctatct atgcatggga tgatactgcg actctttatc gtaagagtga gcgcttgtcg
    15241 ccatcggcga ttttgtcccc gttgcactat acagcaactg aggaaaatcg taataagtta
    15301 cttaatgact tgaaagaaaa acaacctaag gtgattgtgg taaatgacaa ggtggtagtc
    15361 tggtctgaag tggaaacact tttaaaagaa aattaccaac aagtaaagac tgattactca
    15421 gagtttaaag tctataaaat taaataacca aatcaatatc ttgtgtattt ttaaaaattt
    15481 taggattttt aacacaagat attgattttt ctttttagag tggtataata ctttttagaa
    15541 agaacatttt agaaaagagc atgcgtatga ttgcactaga agaaaaaatt acaattttgc
    15601 caactctctt cgtcgagaaa cgagatggga gacgtgttgt atttgatgtg gacaagattg
    15661 acaaggctct ccacaaggcg gctgacaagg ttatggatgt gacacccctg gttgaaaaac
    15721 gcctcaatga tctgactgag cgaattatta cagaaattca tagtcgcttt ccacagggaa
    15781 ttaagattta cgaaattcaa aatatcgtag aacatgaact ccttgaagcc aaagaatatg
    15841 cgctggctga ggagtatatt acttatcgga cacagaggga ttttgagcgc tcaaaagcga
    15901 cggatatcaa ctttagtatt cataaacttc tcaacaaaga ccagacagtt gtcaatgaaa
    15961 atgctaataa agacagtgat gtctttaaca ctcagcgtga tttgacagca gggattgttg
    16021 ggaaatcaat cggactgcaa atgcttccta agcacgtagc caatgcccac caaaaggggg
    16081 atatccacta tcacgatttg gactacagtc cctatacccc tatgaccaac tgctgtttga
    16141 ttgattttaa gggtatgttg gaaaatggtt ttaagattgg aaatgcagag gtagagagtc
    16201 ccaagtctat ccagactgcg acagcacaga tttctcaaat cattgccaac gttgcttcta
    16261 gccagtacgg tggctgttca gctgaccgta tcgatgaaat tttggcgcct tatgcagaga
    16321 agaattatca aaaacatctc aaagatgcag aagagtgggt attgcctgaa aaacgggaag
    16381 attacgcttg gaagaaaacg caaaaggaca tctacgatgc catgcaatct cttgagtatg
    16441 aaatcaatac tctcttcact tcaaatggac aaacaccttt tacttcgtta ggttttggtc
    16501 tgggaaccag tcgttttgaa cgagaaattc aaaaagctat tttaaacatt cgcatcaagg
    16561 gtcttggttc agaacaccgt acggctatct ttcctaaact tattttcact ctgaaaagag
    16621 gccttaattt agaggaagga actcccaact acgacatcaa gcagttggct cttgagtgtg
    16681 caaccaaacg gatgtatcca gatgttttat cttacgataa gattgttgac ttgacaggat
    16741 ccttcaaggt gcctatgggt tgccgttctt tcctccaagg atggaaggat gaaaatggtg
    16801 tagaagtcaa ttcaggtcgc atgaatctgg gtgttgtgac ggttaatctg cctcgtattg
    16861 ctcttgagtc tgaaggtgat atgaataagt tctgggaaat cttcaacgag cgaatgaata
    16921 tcgcagaaga tgctcttgtt taccgtgtcg aacgcactaa agaggcgaca ccagcaaatg
    16981 ctcctattct ttatcagtac ggtgcttttg gccatcgtct aggtaaagaa gaaagtgttg
    17041 accagctctt taagaatcgt cgtgctaccg tttcgctggg ctatatcggc ttgtatgaag
    17101 tagcgacagt tttctttggt aacagctggg aaagtaatcc agatgctaag gaattcacgc
    17161 tagacatcat tcacgatatg aaacgccgtg tagaagagtg gtcagaccaa tatggctacc
    17221 atttctctat ctactcaaca ccatccgaaa gtctgacaga ccgtttctgt cgactagata
    17281 tagacaagtt tggctctatt cctgatatca cagacaagga atactacacc aactctttcc
    17341 actacgatgt tcgtaaaaat ccaacaccgt ttgaaaaatt ggactttgag aaagtctatc
    17401 cagaagcagg tgcgtcaggt ggtttcatcc attattgtga gtatccagtc cttcagcaaa
    17461 atccaaaggc cttggaagct gtctgggatt atgcttatga ccgtgtaggc tatctaggca
    17521 ccaatactcc gattgaccgt tgctacaagt gtgactttga aggggatttt gaaccaactg
    17581 agagagggtt tgcttgtcca aactgtggca atagcgaccc taaaacagta gatgtggtga
    17641 agcgaacttg tggctaccta ggtaatcctc aagcaagacc gatggtcaac ggccgtcaca
    17701 aggaaatcgc tgcgcgtgtc aaacatatga atggttcaac gattaaaata gctgggcatc
    17761 aagtaacaaa ttagaaagaa atgaaatggg aaaatatcaa ctagacgata aggggcgcgc
    17821 acaagtgacc cgttatcacg agaaacactc taaaggtgga gctggtaaga aagaacgctt
    17881 gcttagcttc agagaacaat ttttaaacaa gaacaagaaa aaataaaagt gagagccagc
    17941 tctcgctttt ctcatagtgg gaggtaagga tggaattacg cagaccaaga ttagcggata
    18001 agaaagctgt tttagatatg atgacagagt ttgaaaaatt tcagtcgcct cacgacggcg
    18061 gtttctggga tacagagaac tttgtgtatg aagactggtt agaaagcaat caggaacagg
    18121 aaatggggat taatctgcct gaaggatggg ttcctgcaat tcagttagtg gctttttctg
    18181 agaaaggtca agcagttgga tttcttaatc tccggttgcg cctcagtaac tttctactag
    18241 aagaaggtgg ccacattggc tactccattc gtccatctga aagaggcaag ggttatgcaa
    18301 aagagactct ccgtcagggc ttgcaagttg ctaaggaaaa gaacatcaag aaagctctgg
    18361 tgacctgtag cgtgaataat cctgctagca gagcagtcat tctagcaaat ggtggaatat
    18421 ttgaggatgc tcgcaatgga gtcgagcgtt attggataga ggtagcgaat gaataatcca
    18481 aaaccacaag aatggaaaag cgaggaactt agtcaaggtc gtatcattga ctacaaggcc
    18541 tttaactttg tggacggcga aggcgtgcgt aactctctct atgtatcagg ctgtatgttt
    18601 cactgcgagg gatgttataa tgttgcaact tggtctttta atgctggcat tccctataca
    18661 gcagaattag aagagcagat catggcagat cttgctcagc cctatgttca aggtttgact
    18721 ttgttgggag gggagccttt tcttaatact gggattctct tgccacttgt taagcggatt
    18781 cggaaggaat tgccagacaa ggacatctgg tcctggaccg gctacacttg ggaagaaatg
    18841 atgttggaaa ctccagataa actggaattc ttgtcactga ttgacattct tgtcgatgga
    18901 agatatgatc gaactaagag aaatcttatg ctccagtttc gaggttcatc taaccaacga
    18961 attatcgatg tgcaaaaatc gctcaaaagt gggcaagtag tgatttggga caagctcaat
    19021 gacggaaaag aaagctatga acaggtgaag agagaatgaa gaaaaaatga agaaaaaaga
    19081 cttagtagac caactagtct cagagatcga gacggggaaa gtcaggacac tgggaatata
    19141 cggtcatgga gcttcaggta aatcaacctt tgcacaggaa ttgtaccaag ctttagattc
    19201 tactacagta aatttgctag aaacagatcc ctatatcacc tccggacgct atttggtagt
    19261 acccaaggac gcgccgaatc aaaaggtgac agccagtctg ccagtggcgc atgaactgga
    19321 gagtttgcag agagatatcc ttgccttgca ggcgggtatg gatgtcttga caattgaaga
    19381 accttggaag gctagtgagg tcttgtctgg agccaaacca attttgattg tcgaagggat
    19441 gtctgttggt tttctaccca aggaactctt tgaaaaaacc atctgtttct acacggatga
    19501 ggagaccgaa ttaaagcgac gccttgctag agatacgact gtgagaaatc gctatgcatc
    19561 ctttatattg gctagccatc agatgagacg ggagcagtat ctgcgatact ataaagaaac
    19621 tgagtctaag gcggatatct tagtggacca atcagaagat aaatttgatg tcaagaggac
    19681 ttaacttatg tatagaagaa atatagaaga aaatccctaa ttttagaaag aaaaagtagg
    19741 aaaacagttt catcccaaaa aaacgaaaaa acctaacaaa tcccttgcaa tcgcagggga
    19801 tttgtgttat tctattatgg tgctgtaaat tacagcctta gttttgatgc aagaggttgc
    19861 gacacgctcg gttgcattgc cacgcaacac cgcgtcggtt ttcttgtgga gctagcctat
    19921 tatcttaaat agacgaaaag gagaaaaaga tggcaaacaa aaaaatccgt atccgtttga
    19981 aagcttacga acaccgtacg cttgacacag cggctgcaaa aatcgtagaa tcagctactc
    20041 gtacaggtgc acaagttgcg ggtccaatac cacttccaac tgaacgtagc ctctacacaa
    20101 tcattcgtgc gactcacaaa tacaaagact ctcgcgaaca atttgaaatg cgtacacaca
    20161 aacgtttgat cgatatcgtt aacccaactc aaaaaacagt tgatgccttg atgaaattgg
    20221 atcttccaag tggtgtaaac gtagaaatca aactttaatc taaaatataa aagagcagag
    20281 gctggtgttt caatctaatt gaacacgggc taaactcggt gtgaaaaaga taaacttcct
    20341 cgcgtctgct agacactgtg tcagtttcct attttcactt tgagtttgac gccctttgta
    20401 tcttagactt gagcataaaa aacgctcgtt aaaaactttt tgaataaaaa atatagaaaa
    20461 ggaactattt tctcatgaca aaaggaatct tagggaaaaa agtgggaatg actcaaatct
    20521 tcactgaagc tggcgaattg atccctgtaa cagttattga agcaactcca aacgttgttc
    20581 ttcaagttaa aactgttgaa acagacggat acaacgctat ccaagttggt ttcgatgaca
    20641 aacgcgaagt attgagcaac aaacctgcta aaggacatgt agcgaaagct aacacggctc
    20701 ctaagcgctt cattcgtgaa ttcaaaaacg ttgaaggctt ggaagttggt gctgaaatta
    20761 cagttgaaac attcgcagct ggagacgttg ttgacgtaac tggtacttct aaaggtaaag
    20821 gtttccaagg tgttatcaaa cgccacggac aatcacgtgg accaatggct cacggttctc
    20881 gttaccaccg tcgtccaggt tctatgggac ctgttgcacc taaccgcgta ttcaaaggta
    20941 aaaaccttgc agggcgtatg ggtggcgacc gcgtaacaat tcaaaacctt gaagttgtac
    21001 aagttgttcc agaaaagaac gttatcctta tcaaaggtaa cgtaccaggt gctaagaaat
    21061 ctcttatcac tatcaaatca gcagttaaag ctggtaaata ataaagaaag gggaaatcag
    21121 tcacaatggc aaacgtaaca ttatttgacc aaactggtaa agaagctggc caagttgttc
    21181 ttagcgatgc agtatttggt atcgaaccaa atgaatcagt tgtgtttgat gtaatcatca
    21241 gccaacgcgc aagccttcgt caaggaacac acgctgttaa aaaccgctct gcagtatcag
    21301 gtggtggacg caaaccatgg cgtcaaaaag gaactggacg tgctcgtcaa ggttctatcc
    21361 gctcaccaca atggcgtggt ggtggtgttg tcttcggacc aactccacgt tcatacggct
    21421 acaaacttcc acaaaaagtt cgtcgcctag ctcttaaatc agtttactct gaaaaagttg
    21481 ctgaaaacaa attcgtagct gtagacgctc tttcatttac agctccaaaa actgctgaat
    21541 ttgcaaaagt tcttgcagca ttgagcatcg attctaaagt tcttgttatc cttgaagaag
    21601 gaaatgaatt cgcagctctt tcagctcgta accttccaaa cgtgaaagtt gcaactgcta
    21661 caactgcaag tgttcttgac atcgcaaata gcgacaaact tcttgtcaca caagcagcta
    21721 tctctaaaat cgaggaggtt cttgcataat gaatttgtat gatgttatca aaaaacctgt
    21781 catcactgaa agctcaatgg ctcaacttga agcagggaaa tatgtatttg aagttgacac
    21841 tcgtgcacac aaacttttga tcaagcaagc tgttgaagct gctttcgaag gtgttaaagt
    21901 tgccaatgtt aacacaatca acgtaaaacc aaaagctaaa cgtgttggac gttacactgg
    21961 ttttactaac aaaactaaaa aagctatcat cacacttaca gctgattcta aagcaatcga
    22021 gttgtttgct gctgaagctg aataatctaa ggaggaaata tcgtgggaat tcgtgtttat
    22081 aaaccaacaa caaacggtcg ccgtaatatg acttctttgg atttcgctga aatcacaaca
    22141 agcactcctg aaaaatcatt gcttgttgca ttgaagagca aggctggtcg taacaacaac
    22201 ggtcgtatca cagttcgtca ccaaggtggt ggacacaaac gtttttaccg tttggttgac
    22261 ttcaaacgta ataaagacaa cgttgaagca gttgttaaaa caatcgagta cgatccaaac
    22321 cgttctgcaa acatcgctct tgtacactac actgacggtg tgaaagcata catcatcgct
    22381 ccaaaaggtc ttgaagtagg tcaacgtatc gtttcaggtc cagaagcaga tatcaaagtc
    22441 ggaaacgctc ttccacttgc taacatccca gttggtactt tgattcacaa catcgagttg
    22501 aaaccaggtc gtggtggtga attggtacgt gctgctggtg catctgctca agtattgggt
    22561 tctgaaggta aatatgttct tgttcgtctt caatcaggtg aagttcgtat gattcttgga
    22621 acttgccgtg ctacagttgg tgttgtcgga aacgaacaac atggacttgt aaaccttggt
    22681 aaagcaggac gtagccgttg gaaaggtatc cgcccaacag ttcgtggttc tgtaatgaac
    22741 cctaacgatc acccacacgg tggtggtgaa ggtaaagcac cagttggtcg taaagcacca
    22801 tctactccat ggggcaaacc tgctcttggt cttaaaactc gtaacaagaa agcgaaatct
    22861 gacaaactta tcgttcgtcg tcgcaacgag aaataatatt aaactagtcg cttaagcaac
    22921 tagtaaatcc gccagctcgg tagcgctcca taggagtgca agccgctgtg gtacaacatt
    22981 taaaggagaa aatataaaaa tgggacgcag tcttaaaaaa ggacctttcg tcgatgagca
    23041 tttgatgaaa aaagttgaag ctcaagctaa cgacgaaaag aaaaaagtta ttaaaacttg
    23101 gtcacgtcgt tcaacgatct tcccaagttt cattggttac actattgcag tttatgacgg
    23161 acgtaaacac gtacctgttt acatccaaga agacatggta ggccacaaac ttggtgaatt
    23221 tgcaccaact cgtacttaca aaggtcacgc tgcagacgac aagaaaacac gtagaaaata
    23281 aggagaacat aaatggcaga aattacttca gctaaagcaa tggctcgtac agtacgtgtt
    23341 tcacctcgta aatcacgtct tgttcttgat aacatccgtg gtaaaagcgt agccgatgca
    23401 atcgcaatct tgacattcac tccaaacaaa gctgctgaaa tcatcttgaa agttttgaac
    23461 tcagctgtag ctaacgctga aaacaacttt ggtttggata aagctaattt ggtagtatct
    23521 gaagcattcg caaacgaagg accaactatg aaacgtttcc gtccacgtgc gaaaggttca
    23581 gcttcaccaa tcaacaaacg tacagctcac atcactgtag ctgttgcaga aaaataagga
    23641 ggtaaaatcg tgggtcaaaa agtacatcca attggtatgc gtgtcggcat catccgtgat
    23701 tgggatgcca aatggtatgc tgaaaaagaa tacgcggatt accttcatga agatcttgca
    23761 atccgtaaat tcgttcaaaa agaacttgct gacgcagcag tttcaactat tgaaatcgaa
    23821 cgcgcagtaa acaaagttaa cgtttcactt cacactgcta aaccaggtat ggttatcggt
    23881 aaaggtggtg ctaacgttga tgcactccgt gcaaaactta acaaattgac tggaaaacaa
    23941 gtacacatca acatcatcga aatcaaacaa cctgatttgg atgctcacct tgtaggtgaa
    24001 ggaattgctc gtcaattgga gcaacgtgtt gctttccgtc gtgcacaaaa acaagcaatc
    24061 caacgtgcaa tgcgtgctgg agctaaagga atcaaaactc aagtatcagg tcgtttgaac
    24121 ggtgcagata tcgcccgtgc tgaaggatac tctgaaggaa ctgttccgct tcacacactt
    24181 cgtgcagata tcgattacgc ttgggaagaa gcagatacta catacggtaa acttggtgtt
    24241 aaagtatgga tctaccgtgg tgaagttctt ccagctcgta aaaacactaa aggaggtaaa
    24301 taaccaatgt tagtacctaa acgtgttaaa caccgtcgtg agttccgtgg aaaaatgcgc
    24361 ggtgaagcaa aaggtggaaa agaagtagca ttcggtgaat acggtcttca agctacaact
    24421 agccactgga tcactaaccg ccaaatcgaa gctgctcgta tcgccatgac tcgttacatg
    24481 aaacgtggtg gtaaagtttg gattaaaatc ttcccacaca aatcatacac tgctaaagct
    24541 atcggtgtgc gtatgggatc tggtaaaggg gcacctgaag gttgggtagc accagttaaa
    24601 cgtggtaaag tgatgttcga aatcgctggt gtatctgaag agattgcacg tgaagcgctt
    24661 cgacttgcta gccacaaatt gccagttaaa tgtaaattcg taaaacgtga agcagaataa
    24721 ggagaaggca tgaaacttaa tgaagtaaaa gaatttgtta aagaacttcg tggtctttct
    24781 caagaagaac tcgcgaagcg cgaaaacgaa ttgaaaaaag aattgtttga acttcgtttc
    24841 caagctgcta ctggtcaatt ggaacaaaca gctcgcttga aagaagttaa aaaacaaatc
    24901 gctcgtatca aaacagttca atctgaagcg aaataataga ctagggaagg agaaatttca
    24961 atggaacgca ataatcgtaa agttcttgtt ggacgtgttg tatctgacaa aatggacaag
    25021 acaatcacag ttgtagttga aacaaaacgt aaccacccag tctatggtaa acgtattaac
    25081 tactctaaaa aatacaaagc tcatgatgaa aacaatgttg ccaaagaagg cgatatcgta
    25141 cgtatcatgg aaactcgccc gctttcagct acaaaacgtt tccgtcttgt agaagttgtt
    25201 gaagaagcgg tcatcatcta atcaaacctg aaaggagaaa actgaaatga ttcaaacaga
    25261 aactcgtttg aaagtcgcag acaacagcgg tgctcgcgaa atcttgacta tcaaagttct
    25321 tggtggttca ggacgtaaat ttgcaaacat cggtgatgtt atcgtggcat ctgtaaaaca
    25381 agctactcct ggtggtgcgg ttaaaaaagg tgacgttgtt aaagcagtta tcgttcgtac
    25441 taaatcaggt gctcgtcgtg ctgatggttc atacatcaaa tttgacgaaa acgcagcagt
    25501 tatcatccgt gaagacaaaa ctcctcgcgg aacacgtatc tttggcccag ttgcacgtga
    25561 attgcgtgaa ggtggcttca tgaagatcgt gtcacttgct ccagaagtac tttaattttt
    25621 aggaacaaac tagtccccta gcttcaagct agggtgccct tatgggcgta agaaaaatca
    25681 aggagaaacc taatgtttgt aaaaaaaggc gacaaagttc gcgtaatcgc tggtaaagat
    25741 aagggaacag aagctgttgt ccttactgcc cttccaaaag taaacaaagt tatcgttgaa
    25801 ggtgttaaca ttgttaagaa acaccaacgt ccaactaacg agcttcctca aggtggtatc
    25861 atcgagaaag aagcagctat ccacgtatca aacgttcaag ttttggacaa aaatggtgta
    25921 gctggtcgtg ttggttacaa atttgtagac ggtaaaaaag ttcgctacaa caaaaaatca
    25981 ggcgaagtgc ttgattaatc acgaaggaaa ggagaagtat aatggcaaat cgtttaaaag
    26041 aaaaatatct taatgaagta gttcctgctt tgacagaaca attcaactac tcatcagtga
    26101 tggctgtgcc taaagtagat aagattgttt tgaacatggg tgttggtgaa gctgtatcaa
    26161 acgctaaaag ccttgaaaaa gctgctgaag aattggcact tatctcaggt caaaaaccac
    26221 ttatcactaa agctaaaaaa tcaatcgccg gcttccgtct tcgtgaaggt gttgcgatcg
    26281 gtgcaaaagt tacccttcgt ggtgaacgta tgtacgaatt cttggataaa ttggtatcag
    26341 tttcacttcc acgtgtacgt gacttccacg gtgttccaac aaaatcattt gatggacgcg
    26401 ggaactacac acttggtgtg aaagaacaat taatcttccc agaaatcaac ttcgatgacg
    26461 ttgacaaaac tcgtggtctt gacatcgtta tcgtaacaac tgctaacact gacgaagagt
    26521 cacgtgcatt gcttacaggc cttggaatgc cttttgcaaa ataatatagg aggtaaatct
    26581 aatggctaaa aaatcaatgg tagctagaga ggctaaacgc caaaaaattg ttgaccgtta
    26641 tgctgaaaaa cgtgctgcat taaaggcggc aggggactac gaaggtttat ctaaattacc
    26701 tcgcaacgct tcaccgactc gtttacataa tcgttgtaga gttacggggc gcccacattc
    26761 agtttaccgc aaatttggtc tgagtcgtat cgcttttcgc gaacttgcgc ataaaggtca
    26821 aattcctggt gtaacaaaag catcttggta atttaagata tcaagggcgt caaaactcca
    26881 agtaaaaata ggaaacttga cgaagaaact aaagtttcta ggaaagttta tctttttcac
    26941 acagagttta gcccgggttc aattgggctt gccaatttga acacgagcta cagctttggc
    27001 aaaaaagacc aatttgcttt ggagcattgc ttctgcatta aattgtctat ttttgctcgt
    27061 gctgttacgc tctttgtatc atgtattaac tagcaagtgc aacttgcaaa ctactagtaa
    27121 gaggagaaaa acaaaatggt tatgactgac ccaatcgcag acttcctaac tcgtattcgt
    27181 aatgctaacc aagctaaaca cgaagtactt gaagtacctg catcaaacat caaaaaaggg
    27241 attgctgaaa tccttaaacg cgaaggtttt gtaaaaaacg ttgaaatcat cgaagatgac
    27301 aaacaaggcg tcatccgtgt atttcttaaa tacggaccaa atggtgaaaa agttatcact
    27361 aacttgaaac gtgtttctaa accaggactt cgtgtctaca aaaaacgtga agaccttcca
    27421 aaagttctta acggacttgg aattgccatc ctttcaactt ctgaaggttt gcttactgat
    27481 aaagaagcac gccaaaagaa tgttggtggt gaggttatcg cttacgtttg gtaaaatcaa
    27541 gatacaaagc tcgtaaagaa caaagcaaaa ttaggaagtt ggagaagttt gtttacaaac
    27601 aagccaactt atctattttg cacagttctt agagcgtgtt cagttcagct cttgaactaa
    27661 ataagtatct gaaccccgtg aaaactggcc gttttggcct gacaatttaa caggagaaaa
    27721 taaacatgtc acgtattggt aataaagtta tcgtgttgcc tgctggtgtt gaactcgcta
    27781 acaatgacaa cgttgtaact gtaaaaggac ctaaaggaga acttactcgt gagttctcaa
    27841 aagatattga aatccgtgtg gaaggtactg aagtaactct tcaccgtcca aacgattcaa
    27901 aagaaatgaa aactatccac ggaactactc gtgccctttt gaacaacatg gttgttggtg
    27961 tatcagaagg attcaagaaa gaacttgaaa tgcgtggggt tggttaccgt gcacagcttc
    28021 aaggatctaa acttgttttg gctgttggta aatctcatcc agacgaagtt gaagctccag
    28081 aaggaattac ttttgaactt ccaaacccaa caacaatcgt tgttagcgga atttcaaaag
    28141 aagtagttgg tcaaacagct gcttacgtac gtagccttcg ttcaccagaa ccatataaag
    28201 gtaaaggtat ccgttacgtt ggtgaattcg ttcgccgtaa agaaggtaaa acaggtaaat
    28261 aatgttgagt ggttgatcat caaccaccaa cctattttcc aactttgtgc atagcacacg
    28321 atttaaaact aaagaggtga aaactgtgat ttcaaaacca gataaaaaca aactccgcca
    28381 aaaacgccac cgtcgcgttc gcggaaaact ctctggaact gctgatcgcc cacgtttgaa
    28441 cgtattccgt tctaatacag gcatctacgc tcaagtgatt gatgacgtag cgggtgtaac
    28501 gctcgcaagt gcttcaactc ttgataaaga agtttcaaaa ggaactaaaa ctgaacaagc
    28561 cgttgctgtc ggtaaactcg ttgcagaacg tgcaaacgct aaaggtattt cagaagtggt
    28621 gttcgaccgc ggtggatatc tatatcacgg acgtgtgaaa gctttggctg atgcagctcg
    28681 tgaaaacgga ttgaaattct aataggagga cactagaaaa tggcatttaa agacaatgca
    28741 gttgaattag aagaacgcgt agttgctgtc aaccgtgtta caaaagttgt taaaggtgga
    28801 cgtcgtcttc gtttcgcagc tcttgttgtt gttggtgacc acaatggtcg cgtaggattt
    28861 ggtactggta aagctcaaga agttccagaa gcaatccgta aagcagtaga tgatgctaag
    28921 aaaaacttga tcgaagttcc tatggttgga acaacaatcc cacacgaagt tctttcagaa
    28981 ttcggtggag ctaaagtatt gttgaaacct gctgtagaag gttctggagt tgccgctggt
    29041 ggtgcagttc gtgccgttgt ggaattggca ggtgtggcag atattacatc taaatcactt
    29101 ggttctaaca ctccaatcaa cattgttcgt gcaactgttg aaggtttgaa acaattgaaa
    29161 cgcgctgaag aaattgctgc ccttcgtggt atttcagttt ctgatttggc ataagaaagg
    29221 ggataaaatg gctcaaatta aaattacttt gactaagtct ccaatcggac gcattccatc
    29281 acaacgtaaa actgttgtag cacttggact tggcaaattg aacagctctg ttattaaaga
    29341 agataacgct gctatccgtg gtatgattac agcagtatct cacttagtaa cagttgaaga
    29401 agtaaactaa tgaagtttta ggggatgtgc actgtaccat cccctaaaac tagatatagt
    29461 catctatgat gacatcgtat aggcgagttg atgggggaga caaccttttc tcccttatcg
    29521 gcgctagcat tttacaaaag aggagaaaat aaaaatgaaa cttcatgaat tgaaacctgc
    29581 agaaggttct cgtaaagtac gtaaccgcgt tggtcgtggt acttcatcag gtaacggtaa
    29641 aacatctggt cgtggtcaaa aaggtcaaaa agctcgtagc ggtggcggag ttcgccttgg
    29701 ttttgaaggt ggacaaactc cattgttccg tcgtcttcca aaacgtggat tcactaacat
    29761 caacgctaaa gaatacgcaa ttgtgaacct tgaccaattg aacgtctttg aagatggtgc
    29821 tgaagtaact ccagttgttc ttatcgaagc aggaattgtt aaagctgaaa agtcaggtat
    29881 taaaattctt ggtaacggtg agttgactaa gaaattgact gtgaaagcag ctaaattctc
    29941 taaatcagct gaagaagcta tcactgctaa aggtggttca gtagaagtca tctaagagag
    30001 gtgacctatg ttttttaaat tattaagaga agctcttaaa gtcaagcagg ttcgatcaaa
    30061 aattttattt acaattttta tcgttttggt ctttcgtatc ggaactagca ttacagttcc
    30121 tggtgtgaat gccaatagct tgaatgcttt aagtggatta tccttcttaa acatgttgag
    30181 cttggtgtcg gggaatgccc taaaaaactt ttcgattttt gccctaggag ttagtcccta
    30241 tatcaccgct tctattgttg tccaactctt gcaaatggat attttaccca agtttgtaga
    30301 gtggggtaaa caaggggaag taggtcgaag aaaattgaat caagctactc gttatattgc
    30361 tctagttctc gcttttgtgc aatctatcgg gattacagct ggttttaata ccttggctgg
    30421 agctcaattg attaaaactg ctttaactcc acaagttttt ctgacgattg gtatcatctt
    30481 aacagctggt agtatgattg tcacttggtt gggtgagcaa attacagata agggatacgg
    30541 aaacggtgtt tccatgatta tctttgccgg gattgtttcc tcaattccag agatgattca
    30601 gggcatctat gtggactact ttgtgaacgt cccaagtagc cgtatcactt catctatcat
    30661 tttcgtaatc attttgatta ttactgtatt gttgattatt tactttacaa cttatgttca
    30721 acaagcagaa tacaaaattc caatccaata tactaaggtt gcacaaggtg ctccatctag
    30781 ctcttacctt ccgttaaagg taaaccctgc tggagttatc cctgttatct ttgccagttc
    30841 gattactgca gcgcctgcgg ctattcttca gtttttgagt gccacaggtc atgattgggc
    30901 ttgggtaagg gtagcacaag agatgttggc aactacttct ccaactggta ttgccatgta
    30961 tgctttgttg attattctct ttacattctt ctatacgttt gtacagatta atcctgaaaa
    31021 agcagcagag agcctacaaa agagtggtgc ctatatccat ggagttcgtc ctggtaaagg
    31081 tacagaagaa tatatgtcta aacttcttcg tcgtcttgca actgttggtt ccctcttcct
    31141 tggtgtgatt tccattttac cgattgcagc taaagatgta tttggtcttt ctgatgttgt
    31201 tgcctttggt ggaacaagtc tcttgatcat tatctctaca ggtatcgaag gaatcaagca
    31261 attggaaggt tacctattga aacgtaagta tgttggtttc atggacagaa cagaataaaa
    31321 gtatttactg aatcagtaaa tactgaggga gtggaggttt aaactctgac atttgtaaga
    31381 gttggatctc ccctcttcta ttttgttttt aaataggggt gaaaagactt tttgcttcta
    31441 tttaaaaata aaataaggag atcaaatcat gaatcttttg attatgggct tacctggtgc
    31501 aggtaaggga actcaagcag caaaaatcgt agaacaattc catgttgcac atatctcaac
    31561 aggtgatatg ttccgcgctg caatggcaaa tcaaactgaa atgggtgttc ttgctaagtc
    31621 atatattgac aagggtgaat tggttcctga cgaagttaca aatggaatcg taaaagaacg
    31681 cctttcacaa gatgatatta aagaaacagg attcttattg gatggttacc cacgtacaat
    31741 tgaacaagct catgccttgg acaaaacatt ggctgaactt ggcattgaac tagaaggtat
    31801 tatcaatatt gaagtgaacc ctgacagcct cttggaacgt ttgagtggcc gtatcatcca
    31861 ccgcgtaact ggagaaactt tccacaaggt ctttaaccca ccagttgact ataaagaaga
    31921 agattactac caacgtgaag atgataagcc tgagacagta aaacgtcgtt tggatgttaa
    31981 tattgctcaa ggagaaccaa tcattgctca ctaccgtgcc aaaggtttgg ttcatgacat
    32041 cgaaggtaat caagatatca atgatgtctt ctcagatatt gaaaaagtat tgacaaattt
    32101 gaaataaagc gtttttcgca cttgcaaaaa tccgctacaa atgttatact gagatagtct
    32161 gacttataat tgttgtctct gtgtctagag gcatcgaatc gaaatttatg gaggtgcttt
    32221 tgcgtggcaa aagacgatgt gattgaagtt gaaggcaaag tagttgatac aatgccgaat
    32281 gcaatgttta cggttgaact tgaaaatgga catcagattt tagcaacagt ttctggtaaa
    32341 attcgtaaaa actatattcg tattttagcg ggagatcgtg ttactgtcga aatgagtcca
    32401 tatgacttga cacgtggacg tatcacttac cgctttaaat aatcgaaaaa cttggaggga
    32461 taagaaatga aagtaagacc atcggtcaaa ccaatttgcg aatactgtaa agttattcgt
    32521 cgtaatggtc gtgttatggt aatttgccca gcaaatccaa aacacaaaca acgtcaagga
    32581 taagatagaa aggagaaaac atggctcgta ttgctggagt tgatattcca aatgacaaac
    32641 gcgtagtaat ctcattgact tatgtttatg gtatcggact tgcaacatct aagaaaattt
    32701 tggctgctgc tggaatctca gaagatgttc gtgtacgtga tcttacatca gatcaagaag
    32761 atgctatccg tcgtgaagtg gatgcaatca aagttgaagg tgaccttcgt cgtgaagtaa
    32821 acttgaacat caaacgtttg atggaaatcg gttcataccg tggtatccgt caccgtcgtg
    32881 gacttcctgt ccgtggacaa aacactaaaa acaacgctcg cactcgtaaa ggtaaagctg
    32941 ttgcgattgc tggtaagaaa aaataatata ggaggtaaaa gtcttggcta aaccaacacg
    33001 taaacgtcgt gtgaaaaaga atatcgaatc tggtattgct catattcacg ctacatttaa
    33061 taacactatt gttatgatta ctgatgtgca tggtaatgca attgcttggt catcagctgg
    33121 tgctcttggt ttcaaaggtt ctcgtaaatc tacaccattc gctgctcaaa tggcttctga
    33181 agctgctgct aaatctgcac aagaacacgg tcttaaatca gttgaagtta ctgtaaaagg
    33241 tccaggttct ggtcgtgagt cagctattcg tgcgcttgct gccgctggtc ttgaagtaac
    33301 agcaattcgt gatgtgactc cagtgccaca caatggtgct cgtcctccaa aacgtcgccg
    33361 tgtataatca tcgcattaca ctgcttttcg tttaagaggg agtaactaaa tgatcgagtt
    33421 tgaaaaacca aatataacaa aaattgatga aaataaagat tatggcaagt ttgtaatcga
    33481 accacttgaa cgtggctacg gtacaactct tggtaactct cttcgtcgtg tacttctagc
    33541 ttctctacca ggagcagctg tgacatctat caacattgat ggtgtgttac atgagtttga
    33601 cacagttcca ggtgttcgtg aagacgtgat gcaaatcatt ctgaacatta aaggaattgc
    33661 agtgaaatcg tacgttgaag acgaaaaaat catcgaactg gatgttgaag gtcctgctga
    33721 agtaacagct ggtgacattt tgacagatag cgatattgaa attgtaaatc cagatcatta
    33781 tctctttaca atcggtgaag gttcttctct aaaagcgact atgactgtta acagtggtcg
    33841 tggatatgta cctgctgatg aaaataaaaa ggataatgca ccagttggaa cacttgctgt
    33901 agattctatt tatacaccag ttacaaaagt caactatcaa gtggaacctg ctcgtgtagg
    33961 tagcaatgat ggtttcgaca aattaaccct tgaaatcttg acaaatggaa caattattcc
    34021 agaagatgct ttagggcttt cagcacgtat tttgacagaa catcttgatt tgtttacaaa
    34081 tcttactgag attgctaagt caactgaagt gatgaaagaa gctgatactg aatctgacga
    34141 ccgtatttta gatcgtacga ttgaggaact ggacttgtct gtgcgttcat acaactgttt
    34201 aaaacgtgcc ggtatcaata ctgtgcatga tttgacagaa aaatctgaag cagagatgat
    34261 gaaagtacga aatcttggac gcaagagttt ggaagaagtg aaactcaaac tcattgattt
    34321 gggtcttgga ttaaaagata aataaaggag gaatacatgg cttaccgtaa actaggacgc
    34381 actagctcac aacgtaaagc aatgcttcgc gatttgacaa ctgacctttt gatcaacgaa
    34441 tcaatcgtga caactgaagc tcgtgctaaa gaaatccgta aaactgttga aaaaatgatt
    34501 actctaggta aacgtggtga tttgcatgca cgtcgtcaag cagctgcttt cgtacgtaat
    34561 gaaatcgcat ctgaaaacta tgatgaagca actgataagt acacttctac tacagcactt
    34621 caaaaattgt tctcagaaat cgcacctcgt tatgctgaac gtaacggtgg atacactcgt
    34681 atccttaaaa ctgaaccacg tcgtggtgat gcagcgccaa tggcgatcat cgaattagta
    34741 taaaatcatc aattttgttg agtgttatga tgatggagtc ttgtgctctt agtctagctc
    34801 tggtctaccg ctaggatttc ggtcctagcg ggaacactca tcataagttg ggatagtaga
    34861 cgcttgttta cgaaattgtt tttttcttaa gaacaacttc gtaagcaggc gtttttgagt
    34921 attttcgtta gaattatgct atactatttg aaaagaatcc tgtttaatgt taaggtttct
    34981 tattttaaga agaattggag tttacttatg aaagccatta taactgttgt tggtaaagat
    35041 aaatctggaa ttgttgcagg tgtttctggt aaaattgcag aattaggatt gaatattgac
    35101 gatatctctc aaactgtctt ggatgaatat tttacgatga tggctgttgt atctagtgat
    35161 gaaaagcaag attttaccta tcttcgtaat gaatttgaag cttttgggca aactttgaat
    35221 gtaaaaatca atattcagag tgcagcgatt ttcgaagcta tgtataatat ctaggaggtt
    35281 atcatggata ttagacaagt tactgaaacc atcgccatga ttgaggagca aaatttcgat
    35341 atcagaacca ttaccatggg gatttctctt ttggattgta tcgatccaga tatcaatcgt
    35401 gctgcggaga aaatctatca aaaaattacg acaaaggcgg ctaatttagt agctgttggt
    35461 gatgaaattg cggctgagtt gggaattcct atcgttaata agcgtgtatc ggtgacacct
    35521 atttctctga ttggggcagc gacagatgcg acggactacg tggttctggc aaaagcgctt
    35581 gataaggctg cgaaagagat tggtgtggac tttattggtg gtttttctgc cttagtacaa
    35641 aaaggttatc aaaagggaga tgagattctc atcaattcca ttcctcgcgc tttgactgag
    35701 acggataagg tctgctcgtc agtcaatatc ggctcaacca agtctggtat taatatgacg
    35761 gctgtggcag atatgggacg aattatcaag gaaacggcaa atctttcaga tatgggagcg
    35821 gccaagttgg ttgtattcgc taatgctgtt gaggacaatc catttatggc gggtgccttt
    35881 catggtgttg gggaagcaga tgttatcatc aatgtcggag tttctggtcc tggtgtggtg
    35941 aaacgtgctt tggaaaaagt tcgtggacag agctttgatg tagtagccga aacagttaag
    36001 aaaactgcct ttaaaatcac tcgtatcggt caattggttg gtcaaatggc cagtgagaga
    36061 ctgggtgtgg agtttggtat tgtggacttg agtttggcac caacccctgc ggttggagac
    36121 tctgtggcac gtgtccttga ggaaatgggg ctagaaacag ttggcacgca tggaacgaca
    36181 gctgccttgg ccctcttgaa cgaccaagtt aaaaagggtg gagtgatggc ctgtaatcag
    36241 gtcggtggtc tatctggtgc ctttatccct gtttctgagg atgaaggaat gattgctgca
    36301 gtgcaaaatg gctctcttaa tttagaaaaa ctagaagcta tgacggctat ctgttctgtt
    36361 ggattggata tgattgccat cccagaagat acgcctgctg aaactattgc ggctatgatt
    36421 gcggatgaag cagcaatcgg tgttatcaac atgaaaacaa cagctgttcg tatcattccc
    36481 aaaggaagag aaggcgatat gattgagttt ggtggtctat taggaactgc acccgttatg
    36541 aaggttaatg gggcttcgtc tgtcgacttc atctctcgcg gtggacaaat cccagcacca
    36601 attcatagtt ttaaaaatta agaaaatagg agaaatttta agttctattt aagattagat
    36661 gtgtatacta taatcattaa ataaagacct cctaatatta tttgaaacag ataacactga
    36721 attagtttga atttgatttt catctaatat ctttatttaa tgaactccta aactttttca
    36781 taataatctc cttcaaaagt cgcctgtatg ggtggctttt attttatcat tcatgatata
    36841 atagaagcaa acggaggacg gaaaatggta aaagtacgat tgtatttgat acgtcatggc
    36901 aagaccatgt ttaacacgat tggtcgcgcg caaggttgga gcgatactcc cttaactgct
    36961 gaaggtgaac gagggattca agagttagga attggtttgc gagaatctga tctacagttt
    37021 gagcgcgctt attcgagtga ttctggtcgt accattcaga ccatgggaat tatccttgaa
    37081 gaacttggct tgcaggggaa aatcccttat cgcatggaca agcgtatcag agaatggtgt
    37141 ttcggtagtt ttgatggagc ctatgatggc gatcttttca tgggcattat tcctcgtatc
    37201 tttaatgtgg accacgttca ccaattgtct tatgctgaac tggctgaggg cttggtagag
    37261 gtcgatacag ctggttgggc tgaaggctgg gaaaaactca gtggccgaat caaggaaggc
    37321 tttgaaatga ttgcaaaaga aatggaagat caaggtggag gtaacgccct tgttgtcagc
    37381 catggaatga ctattggaac cattgtttat ctgattaatg gcatgcatcc gcatggtcta
    37441 gataatggta gcgtgacgat tcttgaatat gaggacggtc agtttagggt tgaagttgtc
    37501 ggtgaccgta gttaccgaga gctaggacgt gagaagatgg aagaaggctc tatttaatca
    37561 gtctagactt gcttgccatg agctagggat ttgataagaa tatcaagata agaaaaaaca
    37621 gccgagggta ctcctttcgg ctgtttttga tgtggaaaac taaagtgtaa tgctattgct
    37681 tttagagatt ttcataaaca agagcaagga acctactgtt agaacagtca ggatagttga
    37741 caaggctgcg gctacaccgt aatttcctct gagaacctct gtataaatag ctacagtcat
    37801 tgttcttgtt ttgacattgt agaggaggat agaagtagag agttctgaaa tcagtgtgac
    37861 ccaagatagg atggctccag aaataatatc cgatagcatc attggagttg taatcttagc
    37921 aaagatattg agacgactac ttcctaagct ttcagcagct tcttcaatac ttggtgctat
    37981 ttgttgtaag ctagcaacag atgagcgaat agtataaggt aattttctgg cagatagaga
    38041 cataatcaag atgaaagcag tccctgtaat cataagaaat ccacttccaa atagaccaga
    38101 gaaatgaagg caatccctag aacggttcct ggtacaatat aaggtaccat actgaggctg
    38161 tcaattaagt ttgtaaacaa attccgtttt ctaacggcta ggtaggagat aaatgtcgca
    38221 aatagaacaa ctagaactaa ggcaatcaaa gggatacgaa tggtattgaa aatagcagat
    38281 cccatacgat ggaaagctac cttgtaactg tttggagaat aacctttaac agataccata
    38341 cctgatgttt ttaggaaaga ggtataaatt aagtagattt gaggtaaaac agagataaag
    38401 ataattccgt agactgttgc ataaatggca gctatttttc cttttgtagt ttttttaggc
    38461 tcaattggat ggagcagatt catgctgaaa ctgtagcggt ttgcaatgtg tttttggata
    38521 aggaaaattg ccaaggcaat gataatcgcc ataattgcaa aagcagaatt tcctccaacc
    38581 tcgctaataa attgggtata aatcaggaca gggaaagtcc gatatccttc gccaatcaac
    38641 ataggcgttc caaagtctga gaatgctctc ataaatacaa gcaaggagct gctagtaagg
    38701 ttggaactag gagaggtaaa acaaccgtta cgatacgctt aaatccgaag gacccccatg
    38761 ctttcagctg cttcaagtag agaattgtca atactgttca ttgttccagc aacatataga
    38821 aataccagtg ggaatagttg cagtgtaaag acaagtacaa ttcctttgaa tccataaata
    38881 tcgatagctg gaagatgaag ggcatttgtc aaaaatttag tgatgacccc atttcgtcct
    38941 agcaagagaa cccaggagta ggctcctacg aaaggagctg acatggaagc aatgataatc
    39001 aatatttgta gaaatttctc ccccttgaag tcatacatag agaagagata agctaatagg
    39061 gttcctacaa ctaaggaagt gacagtagcg gtaatggaaa ccttgaaact gttgactagt
    39121 gtctcagagt agtagacttt actaaagaaa gtgacaaaat tagctagtga gaattatcct
    39181 tcatgtataa gtgcttactt gagcacggta atgataggat aaacgagaaa gacaagatag
    39241 gtaagaaaga tgaagaaaga ggaggctgtc caaatattta gttttttaca ttccatggtt
    39301 gactcctttt atcaggtttt gggaaccatc tgcagaaaag atgtttaatt tttgcgtatt
    39361 gattcgtaga cgaatacgat tgtctttttg tagatcttct tcaaaagttg attcttcact
    39421 aacttgaatt tttgaggcaa aacctgtctc aatgaaatac tccgtattta gtccaagata
    39481 gacgctatcg ctaatagttc cttcaatatc tccagattca tctttgataa actcttcggg
    39541 acgaatgctt acatgaatag cttgctcctc aacctgatca agagctggca ttcgaagggc
    39601 atagccatct gaaaagacga tataagcgcc gtcgctccgt ttttcaagat tggcagggat
    39661 aatatttgtg cgtccgataa aggttgccac aaactcatta gctggtttat gatagagttc
    39721 ttttggtcgg ccgatttgtt ggatcacccc atctttcata acagcaattt gatctgaaat
    39781 agccatggct tcttcttggt cgtgggttac ataaacagtt gtaattccca cttcgtgttg
    39841 gatttctcgg atggcttgac gcatatccaa gcgaagtttg gcctccagat tactaagtgg
    39901 ctcgtccatg aggagaacac ttggattaac cgctaaggcg catgccaagg tgacacgttg
    39961 ttgttgtcca ccactgagtt tatcgggctt tcgatccgca tattgagcaa tttgcatgag
    40021 ttcaagatac ttgttggtct gttgaatcaa ttcttctttt ggaaccttct tttgcttaag
    40081 accaaaagca acgttgtctc ggacagtcaa atgtgggaaa atagcgtagt tttggaaaac
    40141 catcccgata ttgcgtttgc tgggttccat attattgatt tttgtatcat cgaagtaaaa
    40201 ttctccatct ttgatactgt tgaaacctgc aatcatacga agaagggtcg ttttcccaca
    40261 tcctgaaggt ccaagaaggg taaagagact tccttttgga attgtaatgt tcaaattctc
    40321 aataacaggg acatcgtggt agattttttg gcgttaataa ttttgatctc actcatagtg
    40381 aacctttttt actgtttaga ttggatatct gtaaagactt cgttgtattt cttaacgata
    40441 tctgatttat tcttgatgac ataatcataa tcttcagtga gtgttttgat tttgtcaatt
    40501 ggtttcatgt tttcgcttgt tttagcattt ttacgaacag gacggttagt agtggttgta
    40561 ccaagtgtat cttgtacttc ttgagagata ataaaatcga taaatttctt gtcattttcc
    40621 atatttttag cttttttaac gatagcagca ctagcaggta ggaagacggt tccttctttt
    40681 ggatagacta ctttaatgtt agctccgtca tttaagagtt taactgctgg atcctcataa
    40741 gagagaccaa cagccatttc tccatcaacg actgctttat agacaccaga tgaacttgaa
    40801 ccgattttat agtgtattga aactagaata gtacacctct acttctaaaa cattgttaga
    40861 aatcgatttg actgtcctga ttaatttgtc ctgttcttat ttcatttcac tatgccatca
    40921 ataagtgtga aaagatcttt tacataagac caagccttat catctttgta accaccttga
    40981 gcttgtagca tatttgttaa ttgagcaaag gcgctagaag agtttgctgg gtcagcagtt
    41041 gcgatttttc cttttagttc aggtttgaga aggtcgctat atccttcgat gttcatgcct
    41101 ttagttaaat cagggttgac gattaaaaca ctaccatcta gtgtataagg agtagagaag
    41161 ccagttgtgt tttgatattc tttgataaca ttatcatttt cttttgaaat atagttttca
    41221 aagagttctc cgtgggtagc atattgtgta taagaaccac caaagataac atcagctaca
    41281 ggaacttctt tttctgactc tagttttttg aaaagttctc cagtaccagc ttgaatcagt
    41341 tctattttga taccatattt ttcttcaaag gcaggaatag ttgctccaat taagccttct
    41401 gagtttggtg aataaacgac tagcgaacca ccgtctcctt tatcagatga actgtcatcg
    41461 gcagattcat tagaagaaca agcagcataa tacatctatt tctttttcat gatggatatc
    41521 tccgttgtgt tatttaagtt tattttaaaa caatgtaagc gtttttaaaa cctacaattc
    41581 tattctatag tgtattgaat ctataacagt acactttgac tgctaaaata tttctataaa
    41641 ttaatttgac tttcctgata gagatgttca catcttattt caattcacta tattagagta
    41701 aaattctcta caaaaagaag aatagcctat ttgactattc ttccgagtga tttcaatttc
    41761 tttggggaaa tatggagata ctttttaaat actttggcaa aatatttata gtctgaaaat
    41821 ccaacttggt cagagatagc gcttaaaggg gagttgggag attcagccat cttatctata
    41881 gcttgtttca ggcgatattg taagatatat tcatttagag tcatggggaa gtcttctttg
    41941 ataaccttat atagatgctt gttatctgca aatagctaga gcatttcaag gaaataaact
    42001 agaaataaca attaatatgg tggttcagaa ataccttttg ataatcaata agatcttcag
    42061 gatgaagtgc gttgattcca tccatttcat atttccgatt taataaacga tatttgtttt
    42121 caccaaattg atgaaaaggg agtagttgaa cttggtcgat atttaatgag ttaaatagag
    42181 tagcgaattt ttctgcatcc tctaaactat tgttaaaatt aggaataact gggattctta
    42241 aaacgatagt tttattttga gaaaaagcat aatgaatgtt tttaataatc atttgattaa
    42301 aaaccccagt cactttttta tgttttatag aattataatg ttttaggtct gtgtagataa
    42361 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
    42421 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
    42481 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
    42541 tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga
    42601 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
    42661 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccgggc
    42721 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
    42781 ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa
    42841 agtttaattt gttgtttttt gctttttatt ttggtagaat aaaacggagg tgtaaaatga
    42901 aaagattgga acaaattatt aaattagtat cagaatatga aaagatcgac gttaatacat
    42961 tatcggaaaa attaaatgta tcgaaagtaa cgattagaaa agatttagat aaattagagt
    43021 caaaaggttt attacacaga gagcatggat atgctgtatt aaatagtgga gatgacttaa
    43081 atgtacgttt gtcaattaac tatgaaatta agagaaaaat tgttcaggaa gcagtaaaat
    43141 tggtgtcaga taatgaaaca ataatgatag aatctggatc gacctgtgct ttacttgctg
    43201 aggaaatttg caagcaaaaa agaaatgtta cgattgtaac aaattcattt tttatagcaa
    43261 attttgtgag agcttatgat tcatgtcgta ttattgttct tggtggagag tttcagaagg
    43321 attcacaggt tactgtggga cctttattaa aagaaatgat acagactttt catgtgcatc
    43381 aagcttttgt tgggacagat ggttacgata aagagatggg ctttacagga aaagatttaa
    43441 tgcgcagtga ggtagttcaa tatatttcag cagcgtcgga taaagtcatt gtactaactg
    43501 actcaagtaa atttgataaa aggggtacag taagaagatt tgctttaagt caagtctatg
    43561 aagtaataac agacgaaaaa ctttctaaac aaaatatagc tacattagaa aatgctggga
    43621 taatggttaa ggtagtttcg taagaggtta aatgtatgaa tcaagatagg aataaactgc
    43681 tctctaaaat tgcttatctg tattatattg aaaacttaaa tcagtcacaa atagcagcaa
    43741 aattaggaat ttatagaacc tctattagta gaatgttaac agaagcaagg aatgcaggaa
    43801 ttgttaaaat tgaaatagag aattttgata ccaatatgtt taagttggaa aattatgtaa
    43861 aagaaaaata tggtctggaa agtttagaaa ttattccaaa tgaatttgat gataatccaa
    43921 caattttatc tgaaagaatt tctcaagttg cagcaggtgt ccttaggaat ctaattgatg
    43981 ataatatgaa aattggcttt tcttggggga aaagtttaag taatttagta gatttaattc
    44041 acagtaaaag tgtccgaaat gttcacttct atcctctagc aggtggtcct agtcacatac
    44101 acgctaaata ccatgtgaat acactgattt atgaaatgtc tagaaaattt catggagagt
    44161 gtacatttat gaatgcaacg attgtgcaag aaaataaatt gttagcagat ggtattttgc
    44221 aatcaagata ttttgaaaat ttgaaaaata gttggaaaga tttagatata gctgtagtcg
    44281 gaattggtga ttttagcaat aaaggaaagc atcaatggtt agacatgctt acagaggatg
    44341 actttaaaga attgaccaaa gttaaaactg taggagaaat ttgttgtcga ttttttgatt
    44401 caaaaggtaa agaagtctat gaaaacttac aggaaagaac gatagcaatc tctttggaag
    44461 atttaaaaaa tattcctcaa agtttagctg ttgcttacgg tgatacgaaa gtatcttcga
    44521 ttctttctgt cttgcgtgct aatttagtaa atcatttgat tacagacaaa aatacaattt
    44581 taaaagtttt ggaagaagat ggggatttga cttttagaga gattctaggt gagtgaaaat
    44641 gatagactga tcagtttatc gttttttctt tttagtcgat tgcacatttg tgctcatata
    44701 aacaaaaata gtttatctgt tgtttttgga ttgacaactt tattatgtag ttgtattcta
    44761 tagttacaaa agaaaatttt aaaatttcaa atgaaaaaag ctttttacat agtgaaatga
    44821 ggaggaattt atggaaatta ttgttccaga tcaaattatc atgggtttaa ttttatatgc
    44881 tggtgatgcg aaacaacata tttataaagc gttagattac ataaaaaatg gtacatgtga
    44941 acggtgtgaa gaagaaatcc agttagctga tgccgcctta ttagaagccc ataatctaca
    45001 aacaaaattc ttggcacaag aagcgtctgg tacaaagaca gaaattacag ctctctttgt
    45061 tcattcacaa gatcatctca tgactagtat gacggagatt aatttaatca aagaaattat
    45121 tagtttgaga aaagaacttc ataaaaaata atactagagt attatcattg ttattaacat
    45181 agaggaggaa aacataatgg tgaagattgg tttgttttgt gcagcaggtt tttctactgg
    45241 tatgcttgta aataatatga aaattgcagc gcaagctagt ggagttgagg cagaaataga
    45301 ggcgttttct cagtctaaat tagcggatta tgcgccaaat atagatgttg cactattggg
    45361 tccacaagtt gcttatacat tagataaatc aaaagaaatt tgtgataagt gtgatgttcc
    45421 gatagctgtt attccgatga tggactatgg tatgttagat ggaaaaaaag tattagattt
    45481 ggccctatct ttgattagtg ggtaagaaaa ggagatttat tatggcaaag atggatgttc
    45541 agaaaatcat tgcaccgatg atgaagtttg tgaatatgcg tggcattata gctctaaaag
    45601 atgggatgtt agcaattttg ccattgacag tagttggtag tttattcttg attatgggac
    45661 aattgccgtt cgaaggatta aataagagca ttgctagtgt ttttggagct aattggacag
    45721 agccgtttat gcaagtatat tcaggaactt ttgctattat gggtctaatt tcttgttttt
    45781 caattgccta ttcttatgct aagaatagcg gagtagaggc tttaccagct ggagttctat
    45841 ctgtatctgc attctttatt ttgctaagat catcttatat ccctaaacaa ggtgaggcaa
    45901 ttggggatgc tattagtaaa gtttggtttg gaggtcaagg aattatcggt gctatcatta
    45961 taggtttggt agtaggaagt atttatacct tctttataaa gagaaaaatt gttattaaga
    46021 tgccagaaca agttccacaa gctattgcca aacagtttga agcaatgatt ccagcatttg
    46081 taattttctt actttctatg attgtatata ttttagcgaa gtcattgact aatggcggaa
    46141 cattcataga aatgatttat tctgctattc aagttccatt gcaaggttta actggatctt
    46201 ggtatggtgc aattggaatt gcattcttta tatcattttt gtggtggttt ggtgttcatg
    46261 gacaatcggt agtaaatgga gtagtgacag ctctgctttt atctaatctt gatgctaata
    46321 aagctatgtt agcctctggt aatctatcat tagaaaatgg tgcacatatt gttactcaac
    46381 aatttttaga ttcattttta attctatcag gttcagggat tacgtttggt cttgtagttg
    46441 ccatgctttt tgcagcaaaa tcaaaacaat accaagcctt aggaaaagtt gccgcttttc
    46501 cagcaatatt taacgtaaat gagccagttg tatttggatt tccgattgtc atgaatccag
    46561 ttatgtttgt acctttcatt cttgttcctg tacttgcagc tgtgatagta tatggagcta
    46621 ttgcaacagg tttcatgcag ccattctcag gggtaacatt gccttggagt acaccagcta
    46681 ttttatcagg atttttggtg ggtggatggc aaggagttat tactcagctg gtgatattag
    46741 cgatgtctac attggtttat tttccattct ttaaagtaca ggatcgttta gcttaccaaa
    46801 atgaaatcaa acaatcttag aggtatttgt gtgttactgt taaactcaca catttgtgct
    46861 aaaaattaga gagttaaaat ttttctagtt aaaagcttga aaatttctat aaaaatcggt
    46921 attatatttt cgaaagaaat aaaaatattt tcgaaagaaa ggtggttacg atggtaaata
    46981 cagaagtagc aagaacaaca atcaagacag aatattttgg cagccttact gaaaggatga
    47041 acaaatatcg agaagatgtt ttaaataaaa aaccttatat tgatgctgag agagcagttc
    47101 tagcaacacg cgcctatgaa cgatacaagg aacaacctaa tgtcctaaaa cgtgcatata
    47161 tgctgaaaga aattttggaa aatatgacta tctatattga agaagaatct atgattgggg
    47221 gaaatcaagc ttcttccaat aaagatgctc ctatttttcc ggaatatacg ctagaatttg
    47281 ttctcaatga gttggatctt tttgaaaagc gtgatggaga tgttttctat attacagaag
    47341 aaacaaaaga acaacttaga agtattgctc cgttttggga aaataataat ttacgtgcta
    47401 gagctggtgc cttattacct gaagaagtgt ctgtttatat ggaaacagga ttcttcggta
    47461 tggaaggtaa gatgaattct ggagatgctc acctagcagt taactatcag aaacttttgc
    47521 aatttggctt aagaggtttt gaagagcggg ctcgtaaagc aaaagcagct ctagatttaa
    47581 cagatccagc aagtattgat aaatatcatt tttacgactc tatatttatc gtaatcgatg
    47641 ctattaaagt atatgcaaag cgctttgttg ctcttgctaa aagtttagcc gaaaatgcaa
    47701 atcctaaacg taagaaagaa ttacttgaga ttgcagatat ttgctctaga gtcccatatg
    47761 aaccggcaac tacttttgca gaagctattc aatcagtttg gtttattcaa tgtattttac
    47821 aaattgaatc taatggccac tctctttcat atggccgttt tgatcaatat atgtatccat
    47881 atatgaaggc tgatttagaa agtggtaaag aaacagaaga tagcattgtt gaacgtctga
    47941 caaatctttg gattaagaca attacaatta ataaggttcg cagtcaatca catacatttt
    48001 cttcagcagg aagtccttta tatcaaaatg ttacaattgg tggacagact cgagataaga
    48061 aggatgctgt taacccatta tcttatttgg tattaaaatc agttgcacaa acccatctac
    48121 cgcaacctaa tctaactgta cgttaccatg caggtttaga tgctcgtttc atgaatgagt
    48181 gtattgaagt gatgaaactt ggttttggta tgcctgcatt taataatgat gagattatta
    48241 ttccttcttt tattgcaaaa ggagtattgg aagatgatgc ttatgattac agtgccattg
    48301 gatgtgttga aacggcagtt ccagggaaat ggggctatcg ttgcacaggt atgagttata
    48361 tgaacttccc taaggttcta cttatcacga tgaatgatgg aattgatccg gcttcgggta
    48421 aacggtttgc accaagcttt ggtcatttta aggatatgaa gaacttttct gaattagaaa
    48481 atgcttggga taaaacacta agatatttga cacgaatgag tgttattgtt gaaaattcta
    48541 ttgatttatc attggaacga gaagttcctg atattctatg ttcagcattg actgatgatt
    48601 gtattggtcg tggaaaacac cttaaagaag gtggagcagt atatgattat atatcaggat
    48661 tgcaagttgg aattgcaaat ttgtcggatt cattagctgc aattaaaaaa ttggtgtttg
    48721 aggaagaacg tataagccca agtcagcttt ggcatgcatt ggaaacagat tatgccggag
    48781 aagaaggtaa ggtcattcaa gaaatgttga ttcatgatgc acctaagtat ggtaatgatg
    48841 atgattatgc tgacaaattg gttactgctg cttatgacat ttatgttgat gaaattgcta
    48901 aatatcctaa tacacgttat ggaagagggc ctattggagg aattcgttat tcaggaacat
    48961 cttctatatc agccaacgta gggcagggac gtggaacatt agcaactcca gatggacgca
    49021 acgcgggtac accgttagca gagggttgtt caccatcaca taatatggat caacacggcc
    49081 ctacatctgt tttaaaatct gtttcaaaat taccaacaga tgaaatcgta ggtggggttc
    49141 tcttaaatca gaaagtaaat cctcaaacgt tagccaaaga agaagataaa ttaaaactaa
    49201 ttgctttgtt acgaacattc tttaatcgtt tacatgggta ccatattcaa tacaatgttg
    49261 tttccagaga gacgttgatt gacgctcaga aacatcctga aaaacacaga gacttaattg
    49321 ttcgtgttgc aggatactct gcattcttca atgttctttc taaggcaacc caagatgaca
    49381 ttataggacg tactgagcat actttgtaaa ataaagaggt tctttttatg gaatttatgc
    49441 ttgacacatt aaatttagat gagattaaaa agtggtctga aattttgccg ctagctgggg
    49501 taacttcaaa tcccactatt gcaaaaagag agggttctat taattttttt gaacgaatca
    49561 aagatgtaag agaattgatt ggctccacac cctctattca tgttcaggtg atttctcaag
    49621 attttgaagg catcttaaag gatgctcatg aaattcgaag acaagcagga gatgatatat
    49681 ttatcaaagt acctgttact ccagctggat tacgtgcaat aaaggtgcta aaaaaagagg
    49741 gctaccatat cactgcaaca gctatttata cagttattca gggattatta gctatcgaag
    49801 caggagcgga ttacctagct ccatattata atagaatgga aaatctgaac attgattcaa
    49861 attctgtcat tcgtcaatta gttcttgcta ttgatagaca gaactctcct agtaagattt
    49921 tggctgcatc ctttaaaaat gtagcacaag taaataatgc tttagctgca ggtgcgcatg
    49981 ctgttacagc aggagcagat gtttttgaat cagctttcgc catgccatct atccaaaagg
    50041 cggttgatga tttttctgac gattggtttg ttactcaaaa tagtcgttcc atttagatag
    50101 agaggaaata catatgagaa tttttgctag tccttctaga tatattcagg gggaaaatgc
    50161 cttgtttgaa aatgccaaat caattttgga tttgggaaat taccctattc tattatgcga
    50221 tcagttggtt tatgatattg ttggaaaacg atttgaagat tatctacata ggtatggttt
    50281 ccatattgtt ctggcgctat ttaatggtga agcttctgac aatgaaatca atcgagttgt
    50341 tgccttggct gagaaagaaa attgtgatag tattatcggt cttggtgggg gaaagacgat
    50401 tgatagcgca aaagctattg cagatttgat tgaaaagcct gttattattg ctccaacaat
    50461 tgcatcgacc gacgcacctg tatctgcttt atctgttatt tatacagatg aaggtgcatt
    50521 tgatcattat ctattttatt ctaaaaatcc agatttagtt ttggttgata caaaagttat
    50581 ttcacaagcc cctaagcgtt tattagcgtc tggtattgca gatggtttag caacttgggt
    50641 tgaggcgcgt gcggttatgc aggcaaatgg aaaaactatg ttgggacaac agcaaacatt
    50701 ggctggagtt gcaattgcga agaaatgtga agaaacgctg tttgcagatg gtttacaggc
    50761 tatggcagct tgtgaagcta aagtggtgac accagcatta gaaaatattg ttgaagctaa
    50821 tactttattg agtggtctag gttttgaaag tggaggatta gctgcggcgc atgcaattca
    50881 taatggtttt actgcattga caggtgacat tcatcattta acacatggtg aaaaagtagc
    50941 ttatggaact ttagtacaac tattattgga aaatagacct aaagaagaac ttgataagta
    51001 tattgagttt tacaaaaaaa ttggtatgcc aacaactcta aaagaaatgc atttggatca
    51061 agttggatat gatgatttaa taaaagttgg taaacaagca actatggagg gtgagacaat
    51121 tcatcagatg ccgtttaaga tttcgccttc agatgttgct caagctatta tcgctgtaga
    51181 tgcctatgta aattcaaaat aaacaataag gactactgtt ttccaaatgg tagtctttta
    51241 ttgatccccg tattgaattc tatagaagat tgaaatagga tgagaacaaa tcgattggga
    51301 aagtaaaatt aatttctata aatgttttag caattgtttc gtactatttc agattcagtc
    51361 tactatacgt tcttcataaa tcaaaaagcg acataggttg tcggctattt attgtgaata
    51421 cattaattag cattccagtt ttatcttcgg tctaaaataa gtattttgtg ctatacgaga
    51481 taagcgtctt gacttactcc ttgatttact gcataacaat gggataaaaa gtgggagata
    51541 gagcaattca tagtcatcaa aattaatgag atacagtata cagtttttcc tttaaacaca
    51601 tttcaaattc cctcaaaaat ggtataatag taacatcaca aaattggaga gagaccatga
    51661 gtttttacaa tcataaagaa attgagccta agtggcaggg ctactgggca gaacatcata
    51721 catttaagac aggaacagat gcatcaaaac ctaagtttta tgcgcttgat atgttccctt
    51781 atccgtctgg agctggtctg cacgtaggac acccagaagg ttatactgca accgatatcc
    51841 tcagtcgtta caaacgtgcg caaggctaca atgtccttca cccaatgggt tgggatgctt
    51901 ttggtttgcc tgcagagcaa tacgctatgg atactggtaa tgacccagca gaatttacag
    51961 cggaaaacat tgccaacttc aaacgtcaaa tcaatgcgct tggattttct tatgactggg
    52021 atcgtgaagt caatacaaca gatccaaact actacaagtg gactcaatgg attttcacca
    52081 agctttacga aaaaggcttg gcctatgaag ctgaagtgcc agtaaactgg gttgaggaat
    52141 tgggaactgc cattgccaat gaagaagtgc ttcctgacgg aacttctgag cgtggaggct
    52201 atccagttgt ccgcaaacca atgcgccaat ggatgctcaa aatcacggct tacgcagagc
    52261 gcttgctcaa tgacttagat gaactagatt ggtcagagtc tatcaaggat atgcaacgca
    52321 actggattgg taaatcaact ggtgccaatg taactttcaa agtaaaagga acagacaagg
    52381 aatttacagt ctttactact cgtccggaca cacttttcgg tgcgactttc actgtcttgg
    52441 ctcctgaaca tgaattagta gacgctatca caagttcaga gcaagcagaa gctgtagcag
    52501 actataaaca ccaagccagc cttaagtctg acttggttcg tacagacctt gctaaggaaa
    52561 aaactggtgt ttggacaggt gcctatgcca tcaaccctgt caatggtaag gaaatgccaa
    52621 tctggattgc ggactatgtt cttgctagtt atggaacagg tgcggttatg gctgtgcctg
    52681 cccacgacca acgtgactgg gaatttgcca aacaatttga ccttccaatc gtcgaagtac
    52741 ttgaaggtgg aaatgtcgaa gaagctgcct acacagagga tggcctgcat gtcaattcag
    52801 acttcctaga tggattgaac aaagaagacg ctattgccaa gattgtggct tgcttggaag
    52861 aaaaaggctg tggtcaggag aaggttacct accgcctccg cgactggctc tttagccgtc
    52921 aacgttactg gggtgagcca attccaatca ttcattggga agatggaact tcaacagctg
    52981 ttcctgaaac tgaattgccg cttgtcttgc ctgtaaccaa ggatatccgt ccttcaggta
    53041 ctggtgaaag tccactagct aacttgacag attggcttga agtgactcgt gaagatggtg
    53101 tcaaaggtcg tcgtgaaact aacaccatgc cacaatgggc tggttcaagc tggtactacc
    53161 tccgctatat tgacccgcac aatactgaga aattggctga tgaggacctc ctcaaacaat
    53221 ggttgccagt agatatctac gtgggtggtg cggaacatgc tgtacttcac ttgctttatg
    53281 ctcgtttctg gcataaattc ctctatgacc tcggtgttgt tccgactaag gaaccattcc
    53341 aaaaactctt taaccaaggg atgattttgg gaacaagcta ccgtgaccac cgtggtgctc
    53401 ttgtggcaac cgacaaggtt gaaaaacgtg atggttcctt ctttcatata gaaacagggg
    53461 aagagttgga gcaagcgcca gccaagatgt ctaaatcgct caagaacgtt gttaacccag
    53521 atgatgtggt ggaacaatac ggtgccgata cccttcgtgt ttatgaaatg tttatgggac
    53581 cactcgatgc ttcgattgct tggtcagaag aaggtttgga aggaagccgt aagttccttg
    53641 accgagttta ccgtttgatt acaagtaaag aaatccttgc ggaaaacaat ggtgctcttg
    53701 acaaggctta caacgaaaca gtcaaagctg ttactgagca aattgagtct ctcaaattca
    53761 acacagctat tgcccaactt atggtctttg tcaatgctgc taacaaggaa gataagcttt
    53821 atgttgacta tgccaaaggc tttattcaat tgattgcacc atttgcacct cacttggcag
    53881 aagaactctg gcaaacagtc gcagaaacag gtgagtcaat ctcttatgta gcttggccaa
    53941 cttgggacga aagtaaactg gttgaagacg aaatcgaaat cgttgtccaa atcaaaggta
    54001 aagtccgtgc caaactcatg gtcgctaaag acctatcacg tgaagaattg caagaaattg
    54061 ctctagcgga tgaaaaagtc aaagcagaaa ttgacggtaa ggaaatcgtg aaagtgatta
    54121 gtgtaccaaa taaattggtt aatattgttg taaaataacc aatttatttg ctgatttcca
    54181 acctcaaaca gtttcccaaa ctgtttgagc caactaaact cgttaatatc gttgtgaaat
    54241 aaggtaggaa tccttcagag tagaatctgg aggatttttt gaatcttctt atgaaagtat
    54301 ggtatactat gggcaactat aaagtttgaa aagtgaaaaa aggagaaagc tatgccagta
    54361 aatgaatatg gtcaaatgat tggggagtca atggaaggtt atacaccagg tgaactgcct
    54421 tctattgatt tcttagaagg gcgttatgct aggatagagg ctctttcagt ggaaaagcat
    54481 gcggaggatt tgctagctgt ttatggcccg gatactcctc gggagatgtg gacctacctt
    54541 tttcaggagc cagtagcaga tatgggggag ctggttagcc ttttacatca gatgttggct
    54601 cgtaaggacc gtttttacta tgcaatcata gacaaggcga ctggtaaggc tttgggatct
    54661 ttttctctca tgcgcattga ccagaataac cgagtaatag aagtcggagc agtcactttt
    54721 tctccagagc taaggggtac acggatagga actgaagctc agtatctcct agctcgctat
    54781 gtttttgagg agctaaacta tcgtcgctac gagtggaaat gcgatgcctt aaatctgcca
    54841 tccagacgag ctgcggagcg tttgagcttt atttatgaag gaaccttccg tcaggcagtg
    54901 gtttataagg gacgtacgag agatacggat tggttgtcta tgattgataa ggactggcct
    54961 caagtcaaag atcgtttgga aacatggttg cgtcctgaaa attttgataa aaatggacag
    55021 cagtacaaga gcttgagaga actttaagag gtgttgagat gattactatt aaaaagcaag
    55081 aaattgtcaa gctagaggat gttttacatc tctatcaggc tgtcggttgg acaaattata
    55141 cccatcaacc agagatgctg gagcaggcct tatctcattc attagtaatt tatctggcac
    55201 ttgatggtga tgctgtggtg ggcttgattc gtttggttgg agatggattc tcatcagtat
    55261 tggttcagga tttaatcgtt ttaccaatct atcagcgtca aggaattggt agtgccttga
    55321 tgaaagaggc tttagaggat tacaaagatg cctatcaagt ccagctggtg acagaagaga
    55381 cagaaagaac cttgggcttc tatcgttcta tgggctttga aatcttatcc acctataatt
    55441 gtatagggat gacttggatg aatcgtaaaa aataacaaaa cttgtttttt cttaagcaaa
    55501 atttaaggat ggtctagtat tatatagtca ttaaataaag acctcctaac tttatttaat
    55561 aaaatcctaa acttttttca tcacaatctc ctaatgaagc cacccaatca ggtggctttt
    55621 ttgcgtggtg aggtgctggt gatagaaatt ttttgcaaaa tagtaaaatt ttagaaaagt
    55681 taagctagtt ttaagtttcg tcttgtatca tgtagttatt aaataaagac ctcctaactt
    55741 tatttaataa aatcctaaac ttttcttttt cataataatc tcccttaact tcacccaatc
    55801 aggtggagtt ttttagctct atttcaggct tttggggact attctaaaaa taatttttcg
    55861 atatttttcg gtatttttcg gattttggtc ggggaattgg cggggacttt tttagcgaat
    55921 ataactaaga aataggtctg tggtcgcttc agctagttca aactcaattt gattgtaagt
    55981 gactgttaat tgaagaactg gaactactgt aataatgatt ttgcttgtct agtagtggcg
    56041 tattaaatga gaagtaacat gtaagattgt ctcttcgttt acgaaatcaa aatttctatg
    56101 aaattgatag aatccctaac gttctcttaa tcttaaccgt cttgtcgtac agcggttgaa
    56161 gaaaatgtaa acctttgaca aaattcgtga actatgggat aataaaaagg aattgagtac
    56221 tagttctata tcataggcta gaaaagaccc caagctcacg accaaataag cttgaggtct
    56281 tttttgacac tatttgtcct tgtttagcca tttatcgact atacgaagaa cgacaccgac
    56341 cacaattggt ccgatgatag ttttgagtac taattccatc atgggctatc tcacctcctt
    56401 tcgtaggcgg tgtagcagtg tcgtagaata ttataccaca taagtgctaa acttaggagt
    56461 cacccaatca ggtggctttt ttgtttgtgg cttggttttt tgatataata gagccatgag
    56521 tagaatttta gataatgaga taatggggga tgaggagtta gtagaacgca cgctccgtcc
    56581 tcagtattta cgtgaatata tcggacagga taaggtcaag gaccagctac aaatctttat
    56641 tgaagctgcc aaaatgcggg atgaagcgct ggatcatgtg ctcttatttg ggcctccagg
    56701 tctcgggaaa acgaccatgg cctttgttat tgccaacgaa ctgggagtca atcttaagca
    56761 gacgtcgggt ccagtcattg aaaaagccgg agatctggta gctattttga atgagttaga
    56821 gcctggggat gtacttttta ttgatgagat ccatcgtttg ccaatgtcag tggaagaggt
    56881 gctttatagt gctatggagg acttctacat cgatattatg attggggctg gtgagggtag
    56941 tcgtagtgtt catttggagt taccaccttt taccttgatt ggtgcgacga ctcgggctgg
    57001 tatgctctcc aatccgctac gggcacgttt tgggattaca ggccatatgg agtattatgc
    57061 ccatgctggc ttgacagaaa ttgtcgagcg gacggcagat atttttgaga tggaaatcac
    57121 tcatgaggca gcatctgagt tggccctacg tagtcgtggg acccctcgta ttgccaatcg
    57181 tctcctcaag cgcgtgcgcg attttgccca gataatgggg aatggggtaa ttgatgatat
    57241 tattaccgat aaggctttga ctatgctgga tgttgaccat gaaggtttgg actatgtgga
    57301 tcaaaaaatc cttcgtacca tgattgagat gtacagtgga ggacctgttg gtctaggaac
    57361 tctttctgtg aatatcgccg aagagcgtga gacagttgaa gatatgtatg agccttactt
    57421 gattcaaaaa ggttttatca tgcggacacg gtctggacgg gtggcgactg ctaaggcata
    57481 tgagcactta ggttatgaat acagtgaaaa ataagcaaga aatcttagag gcttttagag
    57541 aaaatccaga tatgatggcc attctgacga tcatccgaga ccttggtctg aaagactcgt
    57601 ggttggcagc aggttctgtc agaaatttca tttggaatct tttgtcagac aaatcccctt
    57661 ttgatcatga aacagatata gatgtgattt tctttgatcc agatttttct tatgaggaaa
    57721 ccttattact ggagaaaaag ctgcgagagg attttcctca gtaccagtgg gaattgaaaa
    57781 atcaggtcta tatgcatcag cacagtcctc acactgcttc ctataccagt tctcgtgatg
    57841 ctatgagtaa gtatccagaa cggtgtacga cagttggact gcgcttgaat gaagaatcag
    57901 attttgaact ctatgcacct tatggtttgg aggatatttt gaattttcaa gttcgtccaa
    57961 ctcctcattt cttagaaaat gaagaccgaa tggaactcta tcaaacacgt ttatccaaga
    58021 aaaattggca ggagaaatgg aaaaatttga tttttaaaaa tacttaagga aactttaagc
    58081 tagggagtgt acactaagtt cataagttaa gaagacctta acttaaactc ctaaaacttt
    58141 ttcataataa tctccctata aaaataaagt cgcctaatca ggcggcttat ttttttgaaa
    58201 aatgggcttg gtgcctgaga ataaatagct tagtgataga agaaaatggg gaaatatggt
    58261 ataatgaaac gatagatttt tgaataggaa taagatcatg tttggatttt ttaagaaaga
    58321 taaggctgtg gaagtagagg ttccgacaca ggttcctgct catatcggca tcatcatgga
    58381 tggcaatggc cgttgggcta aaaaacgtat gcaaccgcga gtttttggac acaaggcggg
    58441 catggaagca ttgcaaaccg tgaccaaggc agccaacaaa ctgggcgtca aggttattac
    58501 ggtctatgct ttttctacgg aaaactggac ccgtccagat caggaagtca agtttatcat
    58561 gaacttgcca gtagagtttt atgataatta tgtcccggaa ctacatgcga ataatgttaa
    58621 gattcaaatg attggggaga cagaccgcct gcctaagcaa acctttgaag ctttaaccaa
    58681 ggctgaggaa ttgactaaga acaacacagg attgattctt aattttgctc ttaactatgg
    58741 tggacgtgct gagattacac aggcgcttaa gttgatttcc caggatgttt tagatgccaa
    58801 aatcaaccca ggtgacatca cagaggaatt gattggtaac tatctcttta ctcagcattt
    58861 gcctaaggac ttacgagacc cagacttgat tatccgtact agtggagaat tacgtttgag
    58921 caatttcctt ccatggcagg gagcctatag tgagctttat tttacggaca ccttatggcc
    58981 tgattttgac gaagcagcct tgcaggaagc tattcttgcc tataatcgtc gccatcgccg
    59041 atttggagga gtttaggagg aaatatgacc caggatttac agaaaagaac cttgtttgca
    59101 gggattgccc tggctatttt cctaccaatt ttaatgattg ggggcctctt gcttcagata
    59161 gcaatcggaa tcatagccat gctagccatg catgaacttt tgaagataag aggtctagcg
    59221 accatgacga tggagggcct cttgactctc tttgcaacct ttgcattaac cattcccttg
    59281 gagaattacc tgactttttt gccagttgat gggaatgtgg ttgcctatag tgttttgatt
    59341 tcaatcatgt taggaacgac cgtttttagc aagtcttata cgattgagga tgcggttttc
    59401 cctcttgcta tgagcttcta cgtgggcttt ggatttaatg ctttactaga tgctcgtgtt
    59461 gcaggtttgg acaaggctct cttagccttg tgtatcgtct gggcgacaga cagtggtgcc
    59521 tatcttgttg ggatgaacta tgggaaacga aagttagcac caagggtatc gcctaataaa
    59581 acccttgagg gtgccttggg tggtatttta ggagcaattt tagtaaccat tatctttatg
    59641 atagttgaca gtacagttgc tcttccatat ggaatttaca agatgtcagt ctttgctatt
    59701 ttctttagca ttgctggaca atttggtgat ttactagaaa gttcgatcaa acgtcatttt
    59761 ggtgttaagg attctgggaa atttatccct ggacatggtg gtgttttgga tcgtttcgat
    59821 agtatgttgc ttgtatttcc aatcatgcac ttatttggac tcttttaatc aaaagacgga
    59881 ggaaacgcta tgctcggaat tttaaccttt attctggttt tcgggattat tgtagtggtg
    59941 cacgagttcg ggcacttcta ctttgccaag aaatcaggga ttttagtacg tgaatttgcc
    60001 atcggtatgg gacctaaaat ttttgctcac attggcaagg atggaacggc ctataccatt
    60061 cgaatcttgc ctctgggtgg ctatgtccgc atggccggtt ggggtgatga tacaactgaa
    60121 atcaagacag gaacgcctgt tagtttgaca cttgctgatg atggtaaggt taaacgcatc
    60181 aatctctcag gtaaaaaatt ggatcaaaca gccctcccta tgcaggtgac ccagtttgat
    60241 tttgaagaca agctctttat caaaggattg gttctggaag aagaaaaaac atttgcagtg
    60301 gatcacgatg caacggttgt ggaagcagat ggtactgagg ttcggattgc acctttagat
    60361 gttcaatatc aaaatgcgac tatctggggc aaactgatta ccaattttgc aggtcctatg
    60421 aacaatttta tcttaggtgt cgttgttttt tgggttttaa tctttatgca gggtggtgtc
    60481 agagatgttg ataccaatca gttccatatc atgccccaag gtgccttggc caaggtagga
    60541 gtaccagaaa cggcacaaat taccaagatt ggctcacatg aggttagcaa ctgggaaagc
    60601 ttgatccaag ctgtggaaac agaaaccaaa gataagacgg caccgacttt ggatgtgact
    60661 atttctgaaa aggggagtga caaacaagtc actgttacac ccgaagatag tcaaggtcgt
    60721 taccttctag gtgttcaacc gggggttaag tcagattttc tatccatgtt tgtaggtggt
    60781 tttacaactg ctgctgactc agctctccga attctctcag ctctgaaaaa tctgattttc
    60841 caaccggatt tgaacaagtt gggtggacct gttgctatct ttaaggcaag tagtgatgct
    60901 gctaaaaatg gaattgagaa tatcttgtac ttcttggcaa tgatttccat caatattggg
    60961 atttttaatc ttattccgat tccagccttg gatggtggta agattgtgct caatatccta
    61021 gaagccatcc gccacaaacc attgaaacaa gaaattgaaa cctatgtcac cttggccgga
    61081 gtggtcatca tggttgtctt gatgattgct gtgacttgga atgacattat gcgactcttt
    61141 tttagataat cgaggaatat tatgaaacaa agtaaaatgc ctatcccaac gcttcgcgaa
    61201 atgccaagcg atgctcaagt tatcagccat gctcttatgt tgcgtgctgg ttatgttcgc
    61261 caagtttcag caggtgttta ttcttatcta ccacttgcca accgtgtgat tgaaaaagct
    61321 aaaaacatca tgcgccaaga attcgaaaag attggtgctg ttgagatgtt ggctccaacc
    61381 cttcttagtg cagaattgtg gcgtgaatca ggtcgttacg aaacctatgg tgaagacctt
    61441 tacaaactaa aaaaccgtga aaaatcagac tttatcttag gtccaactca cgaagaaacc
    61501 tttacagcta ttgtccgtga ttctgttaaa tcttacaagc aattgccact caacctttat
    61561 caaattcagc ccaagtatcg tgatgaaaaa cgcccacgta atggacttct tcgtacacgt
    61621 gagtttatca tgaaggatgc ttatagtttc cacgctaact atgatagttt ggatagtgtt
    61681 tatgatgagt acaaagcagc ctatgagcgt attttcactc gtagtggttt agacttcaag
    61741 gctattattg gtgacggtgg agccatgggt ggtaaggata gccaagaatt tatggccatt
    61801 acatctgctc gtacagacct tgaccgctgg gttgtcttgg acaagtcagt tgcctcattt
    61861 gacgaaattc ctgcagaagt gcaagaagaa atcaaggcag aattgctcaa atggatagtc
    61921 tctggtgaag ataccattgc ttactcaagt gagtctagct atgcagctaa cttagaaatg
    61981 gcaacaaacg agtacaaacc aagcaaccgt gttgtcgctg aagaagaagt tactcgtgtt
    62041 gcaacgccag atgttaaatc aattgatgaa gttgcagcct tcctcaatgt tccagaagaa
    62101 caaacgatta aaaccctctt ctacattgca gatggtgagc ttgttgcagc ccttctagtt
    62161 ggaaatgacc aactcaacga agtcaagttg aaaaatcact tgggagcaga tttctttgac
    62221 gttgctagcg aagaagaagt ggcgaatgtt gttcaagcag gatttggttc acttggacca
    62281 gttggtttgc cagagaatat taaaattatt gcagatcgta aggtgcaaga tgttcgcaat
    62341 gcagttgtcg gtgctaacga agatggctac cacttgactg gtgtgaaccc aggccgtgat
    62401 tttactgcag aatatgtgga tatccgtgaa gttcgtgagg gtgaaatttc cccagatgga
    62461 caaggtgtcc ttaactttgc gcgtggtatt gagatcggtc atattttcaa actcggaact
    62521 cgctactcag caagcatggg agcagatgtc ttggatgaaa atggtcgtgc tgtgccaatc
    62581 atcatgggat gttacggtat cggtgtcagc cgtcttcttt cagcagtgat ggagcaacac
    62641 gctcgcctct ttgttaacaa aacgccaaaa ggtgaatacc gttacgcttg gggaatcaat
    62701 ttccctaaag aattggcacc atttgatgtg catttgatta ctgtcaatgt caaggatgaa
    62761 gaagcgcaag ccttgacaga aaaacttgaa gcaagcttga tgggagctgg ttatgaagtc
    62821 ttgacagatg accgtaacga acgtgtcggt gttaaattta gcgatagtga cttgattggt
    62881 cttccaatcc gtatcactgt tggtaaaaaa gcggccgatg gcattgtaga agttaagatc
    62941 aaggcaactg gtgacaccat tgaggttcat gcagataacg tgcttgaaac gcttgaaatc
    63001 ctaagcaaga aataaaaact ataatcagaa gaaaaacaag gaaaaaatgt aactagtttt
    63061 taccttgttt tttctgtata atgggagaaa tgagtagata aagaggtaaa tgttatgcta
    63121 agatttccaa aggattttgt ctggggatcc tctacttctg gaccgcagac agaaggacgt
    63181 gtagctggtg acggtaaggg agacaatctc tgggattact ggttccaagt ggagccaaat
    63241 cgttactata atgggattgg ttcagataag acatcgactt tttatgaaaa ttgggagcgg
    63301 gatattgagc ttttgttaga gactggtcac acagtctttc ggacttctat tcagtggtca
    63361 cggatttttc cacaaggctg tggaaaagtc aaccctcaag gtgtggattt ttatcgtaag
    63421 gtctttgagg ctattaaggc taaaggaatt cgtctgttag tcaatctcta tcattttgat
    63481 ttaccttttg cccttcaaga ggatggtgat ggttgggaaa ataaggcgac agtctcagcc
    63541 tatgaagact atgctcgttt ttgttttgag acttatggag atttagtgga tcaatggatt
    63601 acctttaacg agcccatcgt tcctgtagaa tttggttatt tttatgatgc ccattatcca
    63661 cataaggtgg atgcagaggc agctgttaag gtagcctatc atacacaatt ggccagcagc
    63721 cgggctgtta aggcttgcca tgaacttttg cctgattcca agattgggat tgtcctcaac
    63781 ttgacaccgg cttatccacg tagccagcat cctgctgatg tcaaggcagc tcgtattgcg
    63841 gccctttttc aggcccaatc tttcttagat ccatctgtct tggggactta tccacaggag
    63901 ttggtagaaa tcttgcatga acacggtctt ttacctgatg ctacagagga agagttggaa
    63961 ctcattcgtg ataatacggt ggacttcctt ggtgtgaact actatcaacc tttgcgtgtt
    64021 atggctcctc gatttgctaa gcatccagat agtccactct taccagaaca tttttacgag
    64081 ccttatgtga tgcctggacg taaaatcaat tctcaccgtg gttgggagat ttatgagcaa
    64141 gggatttatg acatcgccca aaatatcaag gaaaattatg gcaatattga gtggatgttg
    64201 actgagaatg gtatgggtgt tgaaggggaa gaaaaattcc gtcaagatgg aatgattcaa
    64261 gatgattacc gtattgactt tgtaaaaggt catcttcgtg aacttcaccg tgccattgaa
    64321 gatggtgcca actgtaaggg ctacttgatt tggaccttta ttgactgctg gtcatggctc
    64381 aacagctata aaaatcgcta tggtttggtc gaattagact tggaaacgca agaacgtcgt
    64441 ctgaagaaat caggccactg gttcaaagaa ttaagcgata ataatggatt ttaaaaagga
    64501 ctctgactga ttatcccaat cggtcagagt tttttgctta caggagctta atttgaacta
    64561 taccaatttt tactcttgac aagtgaaaga aacaagtata tactgttttt gctgattcta
    64621 taaaagatga attagactat accaatttta aggagaaagc acagcttgcc tgtgtcgtat
    64681 atactatgtg tggaattgtt ggtgttgttg gaaacacaaa tgcaactgat attttgattc
    64741 aagggcttga aaagcttgaa taccgtggct atgattctgc gggaattttt gtcctagatg
    64801 gtgctgataa ccatttggtg aaggctgttg gtcgtatcgc agaattgtct gccaagacag
    64861 ctggtgttga gggaacaact ggtatcggac atactcgttg ggcaactcat gggaaaccaa
    64921 cggaagacaa tgctcaccca caccgctctg agacagaacg ttttgtcttg gttcacaatg
    64981 gggtgattga aaactacctt gaaattaaag aagaatacct tgctgggcac cacttcaaag
    65041 ggcaaacaga tacggaaatc gccgtacatt tgattggaaa atttgcggaa gaagacggtc
    65101 tctcagttct tgaagccttt aaaaaagctc ttcatattat ccgtggttca tatgcctttg
    65161 ccttgattga ctctgaaaat ccagatgtca tctatgtagc gaaaaacaaa tctccacttt
    65221 tgattggtct tggggaaggc tacaatatgg tctgctcaga tgctatggct atgattcgtg
    65281 aaaccaacca atacatggaa attcatgacc gagagttggt aatcgtcaag gctgatagcg
    65341 tggaagttca agactatgat ggtaacagtc gtgaacgtgc tagctatact gcggaacttg
    65401 acttgtcaga tatcggtaag ggaacttatc cttactacat gcttaaggaa attgatgagc
    65461 aaccaactgt tatgcgtaaa ctcattcaag cctacacgga tgatgctggt caagtagtga
    65521 ttgatcctgc tatcattaag gctgttcaag acgcagaccg catctacatc cttgcagctg
    65581 gaacatctta ccatgcagga tttgcttcta agaaaatgtt ggaagaattg acagatacac
    65641 cagttgaact tggcatctca tctgagtggg gctacggtat gccacttctc agcaagaaac
    65701 cactcttcat ctttatcagc caatctggtg aaacagcgga tagtcgtcaa gttttggtca
    65761 aggctaatga aatgggaatt ccaagcttaa cagtgacaaa cgttccaggt tcaaccctct
    65821 cacgtgaagc caactatacc atgctccttc acgcaggtcc tgaaattgcc gtggcatcaa
    65881 ctaaagccta tacagcgcaa atcgcagccc ttgccttcct tgcaaaagca gtcggagaag
    65941 caaatggtaa tgctaaagcg caagcctttg acctggttca tgaattgtca atcgtagctc
    66001 agtctatcga atcaactctt tcagagaaag aaaccattga agccaaggtt cgtgaacttc
    66061 ttgaaacaac tcgtaacgcc ttttacatcg gacgtggtca agattactac gtagccatgg
    66121 aagcaagtct caaactcaaa gagatttctt atatccagtg tgaaggtttt gcggcaggag
    66181 aactcaagca cggaaccatt gccttgattg aagaaggaac acctgtcttg gctctcttgt
    66241 cagatccagt ccttgccaac cacactcgtg gaaatatcca agaggtggca gcccgtggtg
    66301 ctaaggtcct cactatcgca gaagaaaatg ttgctaaaga taccgacgat atcgtcctta
    66361 cgactgtaca cccttacctc tcaccaattt caatggtcgt accgacgcaa ttggttgctt
    66421 actttgcaac cctccaccgt ggcctcgatg tggataaacc acgtaacctt gctaaatcag
    66481 taacggtaga ataagctaaa aaagtctagt tatctaggct ttttctaatg agcaaattgg
    66541 ttgcttgtac tcaagattcg tgttagaata atttttcaaa ttgaaggaca gagatagaca
    66601 aggagaatca caatggtaga attgggaatt tcaacatttg gggaaataac ggagcttgaa
    66661 gggactggac aaacttacag tcatgctgaa cgcattcgcc agttggtggc agagattgag
    66721 ctggctgaca aggttggttt ggacgtatat gggattggcg agcaccatcg ggcggatttt
    66781 gcagtatcag ccccagagat tgtcctggcc gctggggcag tcaataccaa gaaaatccgt
    66841 ttgaccagtg cagtcagcat tctgtcaagc atggatccga ttcgtttgtt ccaacagtat
    66901 gccactatcg atgctttgtc aaatggacga tcggagatta tggctggaag gggctctttc
    66961 acggaatctt tccctctgtt tggttatgac ttgaaagact acgactctct ttttgatgag
    67021 aaattagact tgcttcagtt agtcaatgaa aagaccaagt tagactggca aggtcgattg
    67081 actcaaacga ttgctggtaa agaagtttat cctcgcccag ttcaggacaa attgcccttg
    67141 tggatagcta caggtggtca tgtcgaatca acagtgaaga ttgctcaggc tggtctaccg
    67201 attgtatatg ctattattgg tggtaatcca cgttatttta aaaagttgat tcaggcttat
    67261 cgtgagattg gaagcgaagc gggccatgct gacaaagact tgaaagtggg agcccattct
    67321 tgggggtgga ttgcggaaga tggtgagcag gcggtgaaag attatttcca tccgactaag
    67381 caagtggtgg atgctatttc caaagaccgt ccgcactggc aggaattgcg ttatgagcaa
    67441 tatttggagc aggtagggcc aaatggcgcc atgtttgttg gcaatccaga tcaggtggcc
    67501 gaaaaattga ttcgcatgat agaggattta ggattggacc gcttcatgct tcatctaccg
    67561 cttggttcta tgcctcatga ccaggttctg agagctattg aactcttcgg aacacaagtt
    67621 gcacccaaag tacgagctta ttttgctatg aaagaggctt aataaaaaat cctaatcaag
    67681 tttattagga caacaaatat tgtacaaaaa ttaatccccc gagctataat gcttggggga
    67741 tttttctata ggagggctag tttagttctc ttttttattt tttagtaaga aaccgagacc
    67801 aaggagcgca aggaggctga ttcctgcaaa taggagtttg ttttcgtttt tgattcctgt
    67861 atttggaagt tcagcttgtt tatctggggt tgcaggtata ttttccttgc tagagttttc
    67921 taccgattcg tttcgaaccg cttcttttac agatgcttct tgtgcttctt gtgctggttg
    67981 ttcagcttgt gaatcagctg tagcttggct aggtttattt tccgcatcag ctactttggc
    68041 atctggttta gtagaatcag gtctagcttc tggagctggg tcttgatgtg cagggtgaga
    68101 agcttcattt tgatgttcag gcttggaagg ggttgagtct ggtttctctt ctgcagttga
    68161 ctcatggcta gttccatttt gagagactcg aagaacagta gctgtaaggg cattcaattt
    68221 caagcctttt tcagtccatt caagtccttt cgggttggca attttgactg atcctgcttg
    68281 gttttcatct gccaaaactt ccgcatttct tagatgtgca aaggcagttc ccaaattaaa
    68341 ttcgcgagct ttttcatccg cattgacaaa gactgcgtag atatcgccgt ttggagcagt
    68401 gatttggtag ccaatcacta catcctcttt ttccacacca ttttggcctg ggacagtgat
    68461 gaggtggaca cggtctttga tatcttggag actcttaagt cggaaggcat ctgtagattg
    68521 acgaagggca atcaaacctt tcatatagtc acggctcttg acattttcag gataagcttt
    68581 accatctgta gccttagtcc agtcaaactt gttgactgcg tcgctagaat cgtaagaatc
    68641 atggatgaag taaggatagt caaatgggtt gccgtcctta tcacgcaaca agtgagattt
    68701 gtttggaacc ttatcctctg ctactggagt cttgtaggct gggtcacgga attgtttagt
    68761 acgtccatat tcctgaccgg agtggataaa tggagttcct tgagctgtca agaccatgag
    68821 atttccaagt cgtaaacgac ggtggatttc agcatagttc tcagccttgc ttgggtcttt
    68881 tttgatagac tgggcaatga tgtcaaagag ggtcaagtta tcatgggctg ctatgtattg
    68941 gatgacatct ccagggctgt cagcttcaaa gttagttggt tgagcaatga gatttttaaa
    69001 gatggtgttg acatcacgct tgccacctgt gataaaggca ggttgacctt cgtttggata
    69061 accagatttg aggttgttac ggatgtcatc tgaaaagaca gcgacagtat cggtatgttt
    69121 catccaatct tggtcagcag ctttagtagg catgttttca tcaccggcat aggttctcca
    69181 accttcacca agcatgatga ggtttggatt gagggcgcgt gcagccttgt aagcttcttc
    69241 gatagaagcg gcgtcatggt ctcccatcat atcgaaacgg aatccatcca ctttgtaggt
    69301 atcaactagg tatttgatag agtcaactag gagccgtttg gtcatatggt gggttgttcc
    69361 caagcgtcca ccaccaaagc tagttcgagg tgtgccatcg gcatccataa agtggtagta
    69421 gtttggctct aggtcttcaa agatatcgac tttggctgtg tggttataaa cgacatctag
    69481 gatagctccc ataccacgtt tgtggatttc gttgatgagg tttttaaatt ctgcgattcg
    69541 tttttctgga ttcttaggat cgcttgagta cataccagtc aaggagaagt agttttgagg
    69601 gtcatatccc cagttgtagt tgctgttgct tgaagcgtag tcagacaagc gttcatggtt
    69661 tttcaattca ttgacaaagt agtaagacaa gactggaagg agctggatat gggttacacc
    69721 caagtctttg agatagtcta gtttttcaat gaaggcttca aaagtcccaa atggtttggt
    69781 caagtctttt gcaatggcag gatctgaagt gaagtcacgc acatgagctt cgtagataac
    69841 ggcgtcttca cgagtcttga aattgtgaat cttaccataa gtcaagtctt gaggtccgag
    69901 tttagctgga tctacaaagg cggctttagc cactttatgg gtatcgtcaa ttttggcatc
    69961 gtcgctattc caagcagcaa gagatttagc gtaaggatcg agtgcaagaa cagttttacc
    70021 ttgacgctcg atttggtatt gataatagta gccagtgaaa tctgtgattc cgagtttgtt
    70081 tgtgctgtct agagtttgtt tccaagttcc tctttcccct ttttcgagag cgacagttcc
    70141 aactactttg tcagggtcat tcttgtcgta gacaacaaca gaaaccttat cagcacttgg
    70201 tgaccaaagg gtcaaatcaa cttgttttcc ttcttctttt aggtcagctc ccagtttgcc
    70261 atcatagctg tatgtctcat ctttcaggtg ccagcttgtt ttggtagtga attggtcgga
    70321 attgtagcta acagtataag ggtgttttgt gtcagagaaa tctccgctgt aggtcacttt
    70381 cttaccagct tcatcgattg caacatcggt aatagttacc ttgtttccta ggtgattagt
    70441 gatgttggag tgtttgagga tatcttcttt tttagcaccg acaagtgttg aaaagctact
    70501 ttcaatgcta gaagtgccta cgtgttgggc tcctgtcata cggatatcat ggacatagta
    70561 tggatttgtg taaatcgatt catcatcgtc ttttaggaaa atttggctat gatttttcaa
    70621 atctgtgaac ttataatttt ctttacggat tttcacgtcg tctccttgtt tgctctcatc
    70681 tagtaataaa aatccaaatt ctcttgcggc ttcattaaga ggaatgtcga tatagcggcc
    70741 atatttgcct gtagccgtaa agtctgttcc gtcaggccat tgagcgctac ttggattttt
    70801 cacatctccc cagtaccaga gagatttctt gtcatagttg ccatctgtgc ggtagtagtt
    70861 gacgcgaaca gttcctgcag gctgtggctc gtaagagaaa accttgtaat cttggtctaa
    70921 ccaagcttcg ttcatttttg gaactagttt ttctacagat ttatcgccgg ttagattttt
    70981 tccagctgta ttgttgatga ggaagctaat tttcttggct tgttctccct ttaatttgac
    71041 atctaggtaa tagccgtagt catctttctt ggcatccttg aaggacaaag ctccgtttgg
    71101 ccagttttca gatggttttt caacatcgtc ccaagtccat agtccttgag catccttgtt
    71161 ttcttcagga agttttttga catggatacg gaagtagtta tcttcgatgg gctcttctgc
    71221 cttagtttca gttgttactg gactagtaga agtagttggc tctgttgcag gtttttcttc
    71281 tgttttctct agggaagcgt ttgcatttct aggtgaagaa atatcacttt cgttcttacc
    71341 agagccagct gctgtatcag taagaggttg ggcaagggtg gttgtatttc tgttgttggg
    71401 ttcgttagtg gttggtgtac tttcgtcgga tgagatagtt ggtgtagcca tggcaagcaa
    71461 gacaaggctt gcgccgataa ggacagaacc agttccattt ttgagggaac ggatgctgta
    71521 gaccattttt ttctcagtgt gagatggtgt ttttctcata atgattctcc aatcaataaa
    71581 tttctatatc agtatagcat gtagtaaaac aatatgcaag cgttttcttt gatttgtgaa
    71641 aaaaattctt acttttgaga agttttctgt actattttat atatcagtat aaatgacgga
    71701 gataaaaatg tcacataagc caagccgtat tattaggaag atgatgtgtt cccagctatt
    71761 tttattcaga aattgatctc taacattacc aataaaaaag aaaaatatct tgacaagcaa
    71821 gggaaaattt tgttacaata atagacggta ctttttactt ttggtctctc aagagtgtac
    71881 agggacgtgc tgacaaatgt tgcaaaagta cacacagatg atatctgtca ccaagtgtat
    71941 catcaccaaa aataaaaaaa cacaggagaa tgtagatgcc tacaattaac caattggttc
    72001 gcaaaccgcg taaatcaaaa gtagaaaaat ctaaatcacc agctttgaac gttggttaca
    72061 atagtcataa aaaagttcaa acaaacgttt cttcaccaca aaaacgtggt gttgcaactc
    72121 gtgttggaac aatgacacct aaaaaaccta actcagccct tcgtaaattc gctcgtgtac
    72181 gtttgagcaa ccttatcgaa gttactgcct acatcccagg tatcggacac aacttgcaag
    72241 agcacagcgt ggtgcttctt cgcggtggac gtgtaaaaga ccttccaggg gtacgttacc
    72301 atatcgtccg tggtgcactt gatactgcag gtgttaacga tcgtaaacaa ggccgttcta
    72361 aatacggtac taaacgtcca aaagcataag gaaaggggat aaagagaaat gagtcgtaaa
    72421 aatagagctc caaaacgtga cgtattgcca gatccgctat acaattcaca actagttact
    72481 cgtcttatca accgcgttat gcttgatggt aaacgtggta ctgctgcttc aatcgtttac
    72541 ggtgcttttg agcaaatcaa agaagctact ggcaacgatg cacttgaagt atttgaaaca
    72601 gctatggaaa acatcatgcc tgtacttgaa gtacgtgcac gtcgtgttgg tggttctaac
    72661 taccaagtcc cagttgaagt tcgtccagaa cgtcgtacaa cacttggact tcgttggttg
    72721 gtaacaatcg ctcgtcttcg tggtgaacac acaatgcaag accgtcttgc aaaagaaatc
    72781 ttggatgctg ctaacaacac tggtgcagca gttaagaaac gtgaagatac tcaccgtatg
    72841 gctgaagcta accgtgcatt cgcacacttc cgttggtaag ataggatgcg aaagcgttaa
    72901 gaaagtccca gagaaaatag ggaatcgaag caggttgcgg ttgcaaccaa tgagattcat
    72961 ctttttctcc agacttttag cttgagctca actaaatcat gatgctaggc acggaaggat
    73021 gcaaggtgaa aataggaaac tgacgcagta ttcaacgaat acaaggagtt ttatcttttt
    73081 cacgcagcat cccgttccag ctcacatcgg ctaactaact ttagcctggg ttcaaattag
    73141 ctaaatcgat tagtattagc tataactcag cttaccatct cgtaagttga aaccaacaat
    73201 agcatgaaaa cattgagaac gggtaggtcc tgcctatccg tttttattaa aatcgtgtta
    73261 taatagaata gaaatcaaaa ataaatagga gaaacaaacc tcatggcacg cgaattttca
    73321 cttgaaaaaa ctcgtaatat cggtatcatg gctcacgtcg atgccggtaa aacaacaact
    73381 actgagcgta ttctttacta cactggtaaa atccacaaaa tcggtgaaac tcacgaaggt
    73441 gcgtcacaaa tggactggat ggagcaagag caagaacgtg gtatcacgat cacatctgct
    73501 gcgacaacag ctcaatggaa caaccaccgc gtaaacatca tcgacacacc aggacacgtg
    73561 gacttcacaa tcgaagtaca acgttctctt cgtgtattgg atggtgcggt taccgttctt
    73621 gactcacaat caggtgttga acctcaaact gaaacagttt ggcgtcaagc aactgagtac
    73681 ggagttccac gtatcgtatt tgccaacaaa atggacaaaa tcggtgctga cttcctttac
    73741 tctgtaagca cacttcacga tcgtcttcaa gcaaatgcac acccaatcca attgccaatc
    73801 ggttctgaag atgacttccg tggtatcatt gacttgatca agatgaaagc tgaaatctat
    73861 actaacgacc ttggtacgga tatccttgaa gaagacatcc cagctgaata ccttgaccaa
    73921 gctcaagaat accgtgaaaa attgattgaa gcagttgctg aaactgacga agaattgatg
    73981 atgaaatacc tcgaaggtga agaaatcact aacgaagaat tgaaagctgg tatccgtaaa
    74041 gcgactatca acgttgaatt cttcccagta ttgtgtggtt cagccttcaa aaacaaaggt
    74101 gttcaattga tgcttgatgc ggttatcgac taccttccaa gtccacttga catcccagca
    74161 atcaaaggta ttaacccaga tacagacgct gaagaaactc gtccagcatc tgacgaagag
    74221 ccatttgcag ctcttgcctt caagatcatg actgacccat tcgtaggtcg tttgacattc
    74281 ttccgtgttt actcaggtgt tcttcaatca ggttcatacg tattgaatac ttctaaaggt
    74341 aaacgtgaac gtatcggacg tatccttcaa atgcacgcta acagccgtca agaaatcgac
    74401 actgtttact caggtgatat cgctgctgcc gttggtttga aagatactac aactggtgac
    74461 tcattgacag atgaaaaagc taaaatcatc cttgagtcaa tcaacgttcc agaaccagtt
    74521 atccaattga tggttgagcc aaaatctaaa gctgaccaag acaagatggg tatcgccctt
    74581 caaaaattgg ctgaagaaga tccaacattc cgcgttgaaa caaacgttga aactggtgaa
    74641 acagttatct caggtatggg tgaacttcac cttgacgtcc ttgttgatcg tatgcgtcgt
    74701 gagttcaaag ttgaagcgaa cgtaggtgct cctcaagtat cttaccgtga aacattccgc
    74761 gcttctactc aagcacgtgg attcttcaaa cgtcagtctg gtggtaaagg tcaattcggt
    74821 gatgtgtgga ttgaatttac tccaaacgaa gaaggtaaag gattcgaatt cgaaaacgca
    74881 atcgtcggtg gtgtggttcc tcgtgaattt atcccagcgg ttgaaaaagg tttggtagaa
    74941 tctatggcta acggtgttct tgcaggttac ccaatggttg acgttaaagc taagctttat
    75001 gatggttcat atcacgatgt cgactcatct gaaactgcct tcaagattgc ggcttcactt
    75061 tcccttaaag aagctgctaa atcagcacaa ccagctatcc ttgaaccaat gatgcttgta
    75121 acaatcactg ttccagaaga aaaccttggt gatgttatgg gtcacgtaac tgctcgtcgt
    75181 ggacgtgtag atggtatgga agcacacggt aacagccaaa tcgttcgtgc ttacgttcca
    75241 cttgctgaaa tgttcggtta cgcaacagtt cttcgttctg catctcaagg acgtggtaca
    75301 ttcatgatgg tatttgacca ctacgaagat gtacctaagt cagtacaaga agaaattatt
    75361 aagaaaaata aaggtgaaga ctaatccgtc ctcactctag aaggaagtca cttagtggct
    75421 tccttttgtc tttagaaaat acctctaaat atggtaaaat agtagaagaa taatgtgagg
    75481 aaaatgaatg tcaaatagtt ttgaaatttt gatgaatcaa ttggggatgc ctgctgaaat
    75541 gagacaggct cctgctttag cacaggccaa tattgagcga gttgtggttc ataaaattag
    75601 taaggtatgg gagtttcatt tcgtattttc taatatttta ccgattgaaa tctttttaga
    75661 attaaagaaa ggtttgagcg aagaattttc taagacaggc aataaagctg tttttgaaat
    75721 taaggctcgg tctcaagaat tttcaaatca gctcttgcag tcctactata gggaggcttt
    75781 ctctgaaggt ccatgtgcta gtcaaggttt taagtccctt tatcaaaatt tgcaagttcg
    75841 tgctgagggt aatcagctat ttattgaagg atctgaagcg attgataagg aacattttaa
    75901 gaagaatcat cttcctaatt tagccaaaca acttgaaaag tttggttttc caacttttaa
    75961 ctgtcaagtt gagaagaatg atgtcctgac ccaagagcag gaagaggcct ttcatgctga
    76021 aaatgagcag attgttcaag ctgccaatga ggaagcgctc cgtgctatgg aacaactgga
    76081 gcagatggca cctcctccag cggaagagaa accagtcttt gattttcaag cgaaaaaagc
    76141 tgcagctaaa cccaagctgg ataaggcgga gattactcct atgatcgaag tgacgacaga
    76201 ggaaaatcgt ctggtatttg aaggggttgt ttttgatgtg gagcaaaaag tgactagaac
    76261 aggtcgtgtt ttaatcaact ttaaaatgac ggactatact tcaagttttt ctatgcaaaa
    76321 gtgggttaaa aacgaggaag aggcccagaa gtttgacctc atcaagaaga attcttggct
    76381 ccgagttcga gggaatgtgg agatgaataa cttcacacgc gatttgacta tgaacgtaca
    76441 ggatctgcag gaagttgttc actatgagcg gaaggatttg atgccagaag gtgagcgtcg
    76501 ggttgagttt catgctcata ctaacatgtc gactatggat gctttgccag aggtcgaaga
    76561 gattgttgca acagctgcta agtggggaca caaggcggtt gctatcacag accatgggaa
    76621 tgtccagtcc tttccacatg gctataaggc ggctaagaaa gcgggaatcc agctgatcta
    76681 tgggatagaa gccaatatcg tggaggaccg tgtccctatc gtctataacg aagtggagat
    76741 ggacttatca gaagcaacct acgtggtctt tgacgtggaa acgacgggac tttcagctat
    76801 ctataatgac ttgattcagg ttgcggcctc taagatgtac aaggggaatg ttattgctga
    76861 atttgatgaa tttatcaatc ctgggcatcc cttgtcagct tttactacag agttaactgg
    76921 aattacagat gatcatgtca aaaatgccaa accactagaa caagttttgc aagaattcca
    76981 agaattttgc aaggatacgg tcctagttgc ccacaatgct gcctttgacg ttggctttat
    77041 gaatgctaat tatgagcgtc atggtcttcc aaagattagt cagccagtta ttgatacgct
    77101 ggagtttgct agaaacctct atcctgagta taaacgccat ggtttggggc ctttgaccaa
    77161 gcgttttggt gtggccttgg aacatcacca catggccaac tacgatgcgg aagcgactgg
    77221 tcgtctgctt ttcatcttta tcaaagaggt agcagaaaaa catggtgtga ccgatttagc
    77281 tagactcaac attgatctaa tcagtccaga ttcttacaaa aaagctcgga tcaagcatgc
    77341 gaccatctat gtcaagaatc aggtaggtct aaaaaatatc tttaagctgg tttccttgtc
    77401 taataccaag tattttgaag gagtgccacg gattccgaga acggttctag atgcccatcg
    77461 agagggcttg attttaggtt cagcctgttc agagggtgaa gtttttgacg tggtcgtttc
    77521 tcaaggtgtg gatgcggcgg ttgaggtggc caagtattat gactttatcg aggtcatgcc
    77581 accggctatc tatgcgccct tgattgctaa agagcaggtc aaggatatgg aggaactcca
    77641 gaccattatc aagagtttga tagaggttgg agaccgcctt ggcaagcctg ttttggctac
    77701 gggaaatgtt cactatatcg aaccggaaga agagatttat cgtgaaatta tcgtccgtag
    77761 tttgggacag ggtgcgatga ttaaccgaac tatcggtcat ggtgaacatg cccaaccagc
    77821 accacttcca aaggctcatt ttcgaacgac taatgagatg ttggatgaat ttgccttttt
    77881 gggagaggaa ctggctcgta aactggttat tgaaaacacc aatgccttgg cagaaatatt
    77941 tgaacccgtt gaagtcgtta agggtgactt gtatacgcct ttcatcgaca aggctgaaga
    78001 aacagttgct gagttgacct ataagaaagc ttttgagatt tatggaaatc cgctgccaga
    78061 tattgttgat ttgcggattg aaaaagaatt aacatccata ctggggaatg gatttgctgt
    78121 gatttatctg gcatcgcaga tgctggtgca acgttctaat gaacggggtt atttggttgg
    78181 ttctcgtggg tctgtcggat ctagtttcgt tgcgaccatg attggaatta cggaggtcaa
    78241 tcctctctct cctcactatg tctgtggtca gtgtcagtac agtgagttta tcacagatgg
    78301 ttcgtacggt tcaggatttg atatgcccca taaggactgt ccaaactgtg gtcacaaact
    78361 cagtaaaaac ggacaggata ttccgtttga gaccttcctt ggttttgatg gggataaggt
    78421 tcctgatatt gacttgaact tctcgggaga agatcagcct agcgcccact tggatgtgcg
    78481 tgatatcttt ggtgaagaat atgccttccg tgcgggaaca gttggtacgg tagctgccaa
    78541 gactgcctat ggatttgtca agggttacga gcgagattat ggcaagtttt atcgtgatgc
    78601 agaagtagaa cgcctcgctc aaggagcggc gggtgtcaag cggacaacag gccaacaccc
    78661 ggggggaatc gttgttattc cgaactacat ggatgtctac gattttacgc ctgtccagta
    78721 tccagcagat gatgtcacgg ctgaatggca gaccactcac tttaacttcc acgatatcga
    78781 tgagaacgtc ctcaaactcg atgtactggg acatgatgat ccgaccatga ttcgaaaact
    78841 tcaggatttg tctggtattg accctaataa aattcctatg gatgacgaag gcgtgatggc
    78901 actcttttct gggactgatg tgctaggggt aacacctgaa caaattggaa cgcctacggg
    78961 tatgttgggg attccagagt ttggaacaaa tttcgtacgt ggaatggtag acgaaaccca
    79021 tccgacaacc tttgcggaat tgcttcagct gtctggtctg tcccacggta ctgatgtttg
    79081 gttggggaat gctcaggatc tgattaagca aggaatagcg gacctatcga ctgttatcgg
    79141 ttgtcgggac gacatcatgg tttacctcat gcatgcgggt ctggaaccta agatggcctt
    79201 taccattatg gaacgggtac gtaagggttt gtggctaaag atttcagaag aggagagaaa
    79261 tggctatatc gaagcaatga aggctaataa ggtgccagag tggtatatcg aatcctgtgg
    79321 gaaaattaag tacatgttcc ctaaggccca tgcggcagcc tacgttatga tggccttgcg
    79381 tgtagcttac ttcaaggttc accatcctat ttattactac tgtgcttact tctccattcg
    79441 tgctaaggct tttgatatca agaccatggg tgcgggcttg gaggtcatca agcgcagaat
    79501 ggaagaaatc tctgaaaaac ggaagaacaa tgaagcctct aatgtggaaa tcgatctcta
    79561 tacaactctt gagattgtca atgagatgtg ggaacgaggt ttcaagtttg gtaaattaga
    79621 tctctaccgt agtcaggcga cagagttcct catcgacggg gataccctta tcccaccatt
    79681 tgtagcaatg gatggtctgg gagagaacgt tgccaagcaa ctggtgcggg cgcgtgaaga
    79741 gggagaattc ctctctaaaa cagaactacg caagcgtggt ggactctcat caaccttggt
    79801 tgaaaagatg gatgagatgg gtattcttgg aaatatgcca gaggataacc agttgagttt
    79861 gtttgatgag ttgttttaaa aaattgctta ataatctatt aaaagaggct aacgtatatc
    79921 caatagattt acattagctt tcttttttgt taaaatagtc tatggaaaga gggtgagagt
    79981 atgtcaaaga tgagtataag catccgtctg gatagtgagg ttaaggagca ggcccaacag
    80041 gtgtttagta atctgggaat ggatatgaca acagctatta atattttcct tcgtcaggca
    80101 attcaatatc agggattacc ttttgatgtt agactagacg aaaatcggaa gttgctccaa
    80161 gcgttaacgg atttagacca aaatcgtaat atgagccagt cttttgaatc agtctcagat
    80221 ttgatggagg acttacgtgc ttaagattcg ttatcataaa cagtttaaaa aagattttaa
    80281 gttggctatg aagcgtggtt tgaaggcaga attattagaa gaagttttga attttctggt
    80341 tcaagaaaaa gaacatcctg ccagatatcg tgatcattca ttgacggcat ccaagcattt
    80401 tcaaggagtt cgtgaatgcc atacccagcc agattggctt ttggtttata aagtagacaa
    80461 gtcggaattg attttaaatt tgctgaggac aggcagtcac agtgatttat tttaatctat
    80521 tttaaggggg ttctcatgaa actaagaata tttgcggaag ataagccggc taagaaggta
    80581 tttgaatatc aattagaact tgctgatcgt acaattcttc tatcgacagc actcttgtca
    80641 ggtgctattg ctttagcagg aatcttttct gctttgaaag aaaaataaaa atagaaaaga
    80701 gaaaacagaa tggttttacc aaattttaaa gaaaatctag aaaaatatgc gaaattgttg
    80761 gttgcgaacg gaattaacgt gcaacctggt cacactttgg ctctctctat tgatgtggag
    80821 caacgtgaat tggcacatct aatcgtgaaa gaagcttatg ccttgggtgc gcatgaggtc
    80881 atcgttcagt ggacagatga tgtgattaac cgtgagaaat tcctccatgc cccgatggag
    80941 cgtttggaca atgtgccaga atacaagatt gctgagatga actatctttt ggagaacaag
    81001 gctagccgtc ttggggttcg ttcatctgat ccaggtgcct tgaacggagt ggacgctgac
    81061 aagctttcag cttctgctaa agctatggga cttgccatga agcctatgcg tatcgcaact
    81121 caatctaaca aggttagctg gactgtagca gccgctgctg gacttgagtg ggctaagaaa
    81181 gtcttcccaa atgctgcgag cgacgaagaa gcagttgatt tcctttggga tcaaatcttc
    81241 aaaacttgtc gtgtctacga agcagatcct gttaaggctt gggaggaaca tgcagccatt
    81301 ctcaagagca aggccgatat gcttaataag gagcaatttt cagcccttca ctacacagcg
    81361 ccaggaacag atttaacact tggtttgcca aagaaccacg tttgggaatc agctggtgct
    81421 gtcaatgcac agggcgaaga attcttgcca aatatgccga cagaagaggt cttcacagcg
    81481 cctgacttcc gtcgtgcaga tggttatgtc acttctacaa aaccgcttag ctacaacgga
    81541 aatatcattg aaggcattaa ggtgaccttt aaggatggac aaatcgtaga tatcactgct
    81601 gagaagggtg atcaggttat gaaagacctt gtctttgaaa atgcgggtgc gcgtgccttg
    81661 ggtgaatgtg ccttggtacc agatccaagt ccaatttctc agtcaggcat tacctttttt
    81721 aacacccttt tcgatgaaaa tgcgtcaaac cacttggcta tcggtgcagc ctatgcgact
    81781 agcgttgttg atggagcgga gatgagcgaa gaggagcttg aagctgcagg gcttaaccgt
    81841 tcagatgttc acgtagactt tatgattggt tctaaccaaa tggatatcga tggtattcgt
    81901 gaggatggaa cgcgggtacc tcttttccgt aatgggaatt gggcaaatta aggagataat
    81961 atgttaggaa gtatgttcgt tggtctccta gtgggatttt tagcaggtgc tatgaccaat
    82021 cgtggagagc gaatgggatg ttttggaaaa atgtttctcg gttggatcgg agcctttcta
    82081 ggtcacttgc tctttggaac ttgggggcca gttttatcag gaacagctat tatcccagcg
    82141 attttaggag ccatgattgt tttagctatt ttttggagac gaggaagtta atttcctaaa
    82201 tctgatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc
    82261 actgctttga ggttgcagat agaactgacg aagtcagcta aaaacactgc tttgaggttg
    82321 cagatagaac tgacgaagtc agctaaaaac actgttttga ggttgcagat agaactgacg
    82381 aagtcagcta aaaacactgt tttgaggttg cagatagaac tgacgaagtc agtaacatat
    82441 atacggcaag gcgacgttga cgcggtttga agagattttt gaagagtatg aaacgaaaag
    82501 gaggttggtc attgtactag cctccttttg atatgatata atagttctat gagattagat
    82561 aaatttttag ttgcctgtgc ggtgggaagt cggactgagg tcaaaaactt gctcaaggct
    82621 gggcgcgtga ctgtaaatgg taaaaaagaa aaatcagcta aattgcagat tgatgaaaaa
    82681 atagatgaga ttcgctttga tgggcaagtg ttggagtatg aagagtttgt ctactacatg
    82741 atgaacaagc ccaaaggagt tatctcagcg actgaggatc ccaagcacag aaccgttctg
    82801 gacttgttgg atgacttggc gcggagtaag gaagtttttc cagtaggacg cttggatatt
    82861 gacacgcatg gtcttttgct cttgaccaat aatggtcagc tggctcatgt tcttctttcg
    82921 cccaagcgtc atgtggacaa gacttatcag gctcaggtca agggaattat gacccaagaa
    82981 gatgtggaga catttatcaa gggcattccg ctcaaagact ttacctgtca gcccgctaga
    83041 ctggagattc tttccacaga tgcagaaaag aatcagagcc aaatccgtgt gaccattgca
    83101 gaagggaagt ttcatcagat caagcgtatg gtgggctact gtggcaagga agtggtggac
    83161 ttgcaacgat tgactatggg aactttagta ttagatgaga acctggaacg aggggaatgg
    83221 cgtcgcttga ccaaggaaga attagaaatt ctccgcgcta atattattta atttagttta
    83281 atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggctgta cttgagtacg
    83341 gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga gtataaattc gaaaaaggtg
    83401 agggagaatg tccttacctt ttatgttttt tagttgcttc ctttgtgaaa agagttataa
    83461 tagactgtag aataaaagga ggaatctatg aacgcgattc aagaatcatt tactgataaa
    83521 ctatttgcca actatgaagc aaatgtaaaa taccaagcga ttgaaaatgc tgccagccac
    83581 aacggaattt ttgcagctct ggaacgtcgc caaagccatg tagacaacac acctgttttc
    83641 tcattggatt taaccaagga caaggtcact aaccagaaag cgtctggtcg ttgctggatg
    83701 tttgcggctc tcaacacctt ccgccacaaa ctcatctcgc aatacaaatt ggagaacttt
    83761 gagttgtcac aagcccacac tttcttctgg gataagtatg agaaatcaaa ctggttcttg
    83821 gagcaagtca ttgcgacttc agaccaagaa ttgactagcc gcaaggttag cttcttactc
    83881 caaacacctc aacaagatgg tggtcaatgg gatatggtcg tttccctctt tgaaaaatac
    83941 ggtgtcgtgc ctaagtcagt ttatcctgag tctgtttcat ctagcagcag tcgtgagcta
    84001 aatgcgatcc ttaataaatt gcttcgtcaa gatgctcaaa tcttgcgtga tttgcttgtt
    84061 tctggtgcag atcaagtgac tgttcaagct aagaaagaag acctcttgca agaaatcttt
    84121 aactttcttg ctatgtcatt aggacttcca ccacgcaagt ttgactttgc ttatcgcgat
    84181 aaagataaca actacaaaag tgaaaaagga atcacaccac aagagttcta caagaaatat
    84241 gtcaatcttc ctttagaaga ctacgtttct gttatcaatg ctccaactgc tgataaacct
    84301 tacggaaaat cttacacagt tgagatgttg gggaatgtgg ttggtagccg tgcagttcgt
    84361 tacatcaacg ttccaatgga gcgcttgaaa gaattggcga ttgcccaaat gcaagcaggt
    84421 gagactgttt ggtttggttc tgatgtcggc cagctcagta accgtaaggc tggaatcctt
    84481 gcgacagatg tttatgactt tgaatcaagc atggacatta aacttactca agacaaggct
    84541 ggacgtttgg actatagcga aagcttgatg acccacgcca tggtcttgac aggtgttgac
    84601 ttggacgaaa atggtaaatc aaccaagtgg aaggttgaaa actcatgggg agacaaggtc
    84661 ggtacagatg gttactttgt tgcctcagac gcttggatgg acgaatacac ataccaaatc
    84721 gttgttcgta aggaattgct gacagcagaa gaacaagctg cctatggagc agaaccaatt
    84781 gtacttgcac catgggatcc aatgggagcc ttggctaaat aaaagcatag aaaaaaggaa
    84841 tcagatttta gaacctggtt cctttttagt tgcttgatta catgatgtga agaacatgtg
    84901 ccacaatacc cactgcgaag agggcaagga taatagtgat tggagatact tttttcttaa
    84961 gtaagtacat gcaaagtaaa gtaaggagta gtcctgataa tccaggaatc aacatatcca
    85021 agttttgttg gaaagtagta actttttcag gagtttgaga caatccttgt cctacttgtg
    85081 cgaatgcttc ttggatacct ttagaccctt ctggcaattt atcccaatgg atataagcct
    85141 tttcatctag ttgaacttta gaaacatcga aagcaaattt aatatttacc cagcgttgaa
    85201 caaggacagc aagaatgaac atcccaagga tagaagctcc tttagtgata tcttgaagga
    85261 taccaccaga catatcttta gtgatttctg atccagcctt gtatccaatc tcttgaacat
    85321 accacaagaa tgacatacga atcaagttcc atgcaacaaa gaagaggagt ggccccaaga
    85381 tattgccagt aagggcaagt gaagcaccga gagatccaag gattgggcgt actgtaaacc
    85441 agaatactgg gtcaccgata ccagcaagag gtcccatcat accgatttta accccttgga
    85501 tagcagcgtc atcgatttcc acaccgttag cacgttcttc ttcaagcgca agagtaaccc
    85561 ccatgactgg agcagctacg tatggatgag tgttgaagaa ctcaaggtga cgctcaagag
    85621 cagcgatttg atcttctttt ttagtataga gttttttgat agctggaatg agtgtataag
    85681 cccagcccaa gttttgcatc cgttcaaagt tccaagaccc ttgtaagaag gttgaacgcc
    85741 accaaacttt tttacgatct gatttagtta attgaagttt ttcagtcatg atgttttcag
    85801 tcctttctta tcttagtagt cttctaggat atcgccgatt gggtcgttag aagttgcggc
    85861 tcctccgcca ccatttccac cagttttaga aaggtgaagg tagataagag cgatagcaac
    85921 accgatagca ccgaatccga ttagagtaat atctgacaca gcagcgagaa cgaaaccaag
    85981 agcgaagaat ggccatactt cacgagttgc catcatgttg ataaccatgg cgtaaccaac
    86041 ggcaacgacc ataccaccac cgatagccat accatctttg agccagtctg gcatggcact
    86101 aaggatactt tgtacagttt cagttggtac cataaggaga agagctgcag gaagcgcgat
    86161 acgaagtcct tggaagagta gcgcaatgaa atgcgcacgc tccacagcgc cgaagtcacc
    86221 ttttttagcg gcagcatctg cagtatgaac caaaccaact gaaattgtac gaacaatcat
    86281 tgtcaagaaa agtccagcta cggcaagagg gatagcaacc gcttgggcaa caccgatacc
    86341 agtcttggta aagtcaccac caagaaccat gataatggca gcagcgacag aagcaagtgc
    86401 agcatcagga gcgatagcag caccgatatt tgaccaacca agggcaatca tttgaagcga
    86461 tccaccgagg ataatccctg cttccaagtg acctgttaca agcccaataa gggtacaggc
    86521 tacaagtggt tggtgaaatt ggaactggtc gaggatgcct tcaagacctg caaagaaggc
    86581 tacaacgact actaaaacca tagaaataat agacatgttt aaaatccttt cataaataat
    86641 ggcttatttg acattggctt tgttaatcaa gtcaaacaaa tcttttttag aatcatttgg
    86701 tactttacgg acatcaaatt caacacccaa gtcacgcatt ttttcaaatg tagcaacatc
    86761 ttctttgtcc atagacaaaa cggtattgac caatgtttta cctgttgagt gagccataga
    86821 accaacgtta agagtcttga ttggcacgcc gccttcgatg gcacgaaggg catcttgagg
    86881 tgtttcaaac aagataaggg catgtgtttc tccaaaacgt gggtcttttg aaatctcaat
    86941 cagtttttga attggaacca cgttagcctt gacattacct ggagctgctt gtttaatcaa
    87001 ttctttacga aggtcgtctt tagccacgtt atctgaagca acgatgatac gatttgcttt
    87061 tgaatctgga gtccaagcag ttgcaacctg accgtgaagt agacgtgtgt caagacgggc
    87121 aagattgatt ttcaatttac cgtctccgat aacagttcct tctgggatag cagtttgggc
    87181 aactggagca gctgcagcgc ttgcaacttc ttcaactgga tttagctctt ctggaagagc
    87241 tttgatgcca tctttggctt ctttaatgat attagcagcg actttttcta cacctgcagc
    87301 agcgtccatg aggcgctctg tgtaggcttg aatcaacatc ggtaagttaa gtcctgtgat
    87361 gatggcaaac ttacgctcag gattttctcc catcacgcga ctagcttggt taaatggaga
    87421 accactccaa aggtcagcca aaactagaac ctcatcttct gcgtcaaatg cagcaacagc
    87481 gttattaaac ttagcgtata gatcatcagg accttcattt ggcataaagg tcacaacttg
    87541 aaccttttct tgttcaccaa agatcataga tcctgactga tgaatacccg cagcaaattc
    87601 gccgtggctc gcaataatga ttccgatact cattattgtc attcctcctt ttttgtttta
    87661 gttttcattt aagaaaactt taagactgtt taagtataaa ccgttttcat aaaaaaagca
    87721 actatttatc ataaaaaatt aaaaaatttt tatttttgta tgttgatatg ttgagttttt
    87781 gaggagtaat cttgaattct tatttaaaat ttaatataaa aaagttggaa gaagcttcca
    87841 acttttaaaa taggtttcta ttagattagt gggtgaagtc gagtaccata cgtccttgga
    87901 tttggccttt ttccatttcg tcgaaaatgg caacagcatc ttctactgga cgtttttgaa
    87961 caactgggac taccagacct tctgcaccaa attggaaggc ttcttctaag tctttacgag
    88021 ttccgacaag agaaccgatg acttggattc catcgaggac tgttttaacg atgctgagtt
    88081 ccatcatttc agaaggaaga ccaacagcga cgacgcgacc accagcacga atggagtcaa
    88141 cagcctggtt gaaggcaact ttagacacag cagttacgac agctgaatga gctcctccat
    88201 cagttttttc tttaatgagt ccagctacat cttcaacttc gaggccgtta atcacaatgt
    88261 cagcgcctac ttcttttgca agggcaagtt tgtcattgtt gatatcgact gcgataacat
    88321 gagcattgaa tactttttta gcgtattgaa cagcgaggtt accaagtcca ccagcaccgt
    88381 aaagaacagc ccattggcct ggttcaactt ttgcttcttt gatagcttta taggttgtta
    88441 ctccagcaca tgtgatagaa gaagcttggg ctggatcaag tccgtcagga actttgacag
    88501 catagtcagc agttacgata cattgttcag ccataccacc gtctactgag tagccagcat
    88561 ttttcactgt acggcaaagg gtttcgcgac cagttgtaca gtattcgcaa gtgccacatc
    88621 cttcaaagaa ccaagcaacg ctgacgcggt caccgacttt aaggcttttc acatctggag
    88681 caatctcttt aacgataccg ataccttcgt gcccaagaac acgtcctggg acttgaccaa
    88741 agtcaccatg agcaacgtgg aggtcggtgt ggcaaacgcc acagtattca acttctacaa
    88801 gtgcttcccc agtttcaagt ggacggagta ctttttcttc aatagcaaca ccagtgcttt
    88861 ctggatttac aacaacagct ttcatagcta tcctccttga tattgaccga gaacagggac
    88921 aacgggactg gattgatttt ttgtcaacag agtcgagtgt gacgagctct cttgtattta
    88981 tatgtgaagt atatcacaaa agaaagcctt ttccaaggtt ttggagacaa aaagtagaac
    89041 aatcgattaa ctggttgatg agactgtgaa aagagcacaa agaccagctt gcaagctggt
    89101 catactcttc gaaaatctct tcaaaccacg tcagcttcat ctacaacctc aaagcagtgc
    89161 tttgagcaac ctacggctag cttcctagtt tgctctttga ttctcattga gtatcagtat
    89221 aggttataag aataagttat aagaataagt cttgtagggt tttggcaacc ccatcttggt
    89281 cgttggtaag ggaaatttgc tcatctgcat aagggagtag ctctggattg gcatttttca
    89341 tggcataacc cttcccagca aaagcgagca tttcggtatc attgtgttca tctccaaagg
    89401 caatcaaatc ttttttgtca cgattcatta tcttgagcaa gtagtccaaa gcaaaggcct
    89461 tgttgacacc ttttggggta cattcaagga tattgagcgg acctccccag gtattgatag
    89521 aaagttgatg ctggtagaag gcgttcattt cttttgccaa ggcatatttg tcactggctc
    89581 tggtctgcaa gaggatacag ttagggtcct tggtcaccaa ttcaggctgg aattgatctt
    89641 caggctggaa agcttctaca ccaaatagtt tgggattggc aatttcttca ttgggatttg
    89701 taatgtagaa ttttttacga tattctccag cgataaaatc ggcttgaatg tcctctgaac
    89761 gttgaaccat atctagcaga tattttttgt ctacagtcaa acacttttca aaatcccaaa
    89821 cttggtctgg taaatgagta agggatccgt tgaagttaat cataggagtg tctaagccca
    89881 gttcacggta aaaatctttt gacatacggt aagggcgacc tgtcgtaata ataacctgat
    89941 ggcctttttc agcaactttt ttgatggttt ttttggtaaa gtcagaaatt tgactgtctg
    90001 agttgagcag ggttccatct aggtcaactg caataatttt tttcgtcata tggacctttc
    90061 tagtgtgttt tcagataaga tgtcttctat tataacagaa agcaggcaga aaagcagatt
    90121 ccaaacaaaa aatgaaattg gattaatttc ttaaacaaat caaacaaaga cattgaaaaa
    90181 gtgaatgata aatgatataa ttctattatt gttcgtaaaa attaaaagga gattgatgat
    90241 ggacaaatta tttaaactaa aagagaacgg tacagacgtt cgtacagagg ttctcgctgg
    90301 tttaacaact ttctttgcaa tgagctatat tctctttgta aacccacaaa ttctttcgca
    90361 aacaggaatg cctgctcagg gtgtgtttct tgcaacgatt atcggtgcag ttgctggtac
    90421 cttgatgatg gccttctatg ctaacttgcc atacgctcaa gcaccaggta tgggactcaa
    90481 tgccttcttt acctttacag tcgtatttgg acttggttat tcatggcaag aagccctagc
    90541 tatggtcttc atctgtggga ttatttcatt ggttattacc ttgacaaatg ttcgtaaaat
    90601 gatcattgaa tcgattccaa atgcccttcg ctcggctatt tcagctggta tcggtgtctt
    90661 ccttgcctat gtagggatta agaatgctgg acttttgaaa tttacgattg atccaggcaa
    90721 ctatactgtt gtaggagaag gggctgacaa agctcaagca acgattgcag caaactcttc
    90781 agcagttcca ggcttggtca gctttaataa tccagctgtt ttagtggctc ttgcaggact
    90841 tgccattact atcttctttg tcatcaaagg gattaaaggg ggaattattc tctctatctt
    90901 gacaacaact gttcttgcta ttgcagttgg tttggttgat ttgtctagta tcgattttgc
    90961 taataaccat gttggtgcag cttttgaaga tttgaagaca atctttggtg cagctcttgg
    91021 ttcagaaggt ttgggagctt tggtttcaga tacagctcgc ttgcctgaaa ctctgatggc
    91081 cattcttgcc ttctcattga cagatatttt tgacacaatt ggtaccttga tcggtacagg
    91141 tgaaaaagtt ggtattgtag cgacaaatgg tgaaaatcac caatcagcca aattggataa
    91201 ggctctttac tctgatttga ttggaacgac agtcggtgcc attgcaggta cttcaaacgt
    91261 aacgacttat gttgagtctg ctgctggtat cggtgcaggt ggacgtactg gtttgacagc
    91321 cttggttgta gctatctgtt ttgcgatttc aagcttcttt agcccacttc tagcgatcgt
    91381 accaacagcg gctacagctc caatcttgat tatcgttggg attatgatgc ttggtagctt
    91441 gaaaaatatc cattgggatg atatgtctga agcagttcct gccttcttca catctatctt
    91501 tatgggattc agctactcta tcactcaagg gattgcagtt ggtttcttga cttacacttt
    91561 gactaagctt gtcaaaggtc aagttaaaga tgttcatgcc atgatttgga ttttggatgc
    91621 cttgtttatc cttaactaca tcagcatggc cttataatag aatgacccag ggggatttcc
    91681 cccctttttt aatacaaagg agataggtga tgaaagagaa aaatatgtgg aaagaattgt
    91741 tgaatcgtgc aggctggatt ttggtctttt tacttgccgt ccttttatat caggttcccc
    91801 tagtggttac ctctattttg actttaaaag aagtagccct gctacagtca gggctgatag
    91861 ttactggcct ttcaattgtg gttctggctc tatttattat gggagctcgt aaaaccaagt
    91921 tagctagttt taatttttct ttttttagag ctaaagattt ggcacgtttg ggcttgagtt
    91981 atttagttat tgtcgggtca aatatacttg gttctatctt gttgcaactg tcaaatgaga
    92041 cgacaacagc taaccagtct cagattaacg atatggttca gaatagttct ctgatttcta
    92101 gcttcttctt gctagccttg cttgctccaa tttgtgagga aatcttgtgc cgtgggattg
    92161 ttcctaaaaa gattttccga ggcaaggaga acttgggatt tgtagtcggt acgattgtgt
    92221 ttgctttatt gcatcaacca agtaatttac cttctttatt gatttatgga ggtatgtcga
    92281 cagttctatc ttggacggcc tacaagaccc aacgtttgga aatgtcgatc ttgcttcaca
    92341 tgattgttaa tgggattgct ttctgtttgt tggctcttgt ggtgattatg agtcggacat
    92401 taggaatttc tgtttaaaag tttttatgta ggaaccgacc tctttctact agggaaagat
    92461 gaatgcaatc gtgtccatct ttttcttttt atggtaaaat agaaaaataa tatgatgaaa
    92521 atccttgagg gagtgaccga tatgtcaagt aaagccaatc atgcaaagac agttatttgc
    92581 ggaattatca atgtaacccc agactccttt tcggacggtg gtcaattttt tgctcttgag
    92641 caggcactcc agcaggctcg taaattgata gcagaaggag ccagtatgct cgatatcggc
    92701 ggagaatcga ctcggccggg aagtagctat gttgagatag aagaggaaat ccagcgtgtt
    92761 gttccagtga tcaaagcgat tcgcaaggaa agtgatgtcc tcatctctat tgatacttgg
    92821 aagagtcaag tagcagaggc tgctttggct actggtgccg atctagtcaa tgatatcact
    92881 ggtctcatgg gtgatgaaaa aatggcccat gtggttgcta aagctggagc gaaagtggtc
    92941 atcatgttta acccagttat ggctcgacct cagcatccta gttcgcttat cttccctcat
    93001 tttggctttg gtcaaacctt tacagaaaaa gagttagctg actttgaaac attgccaatc
    93061 gaagacttga tggtggcttt ctttgaacga gcactagcga gagcggcaga agctggtatt
    93121 gcaccagaaa atatcctgtt ggatccagga attggctttg gtctgaccaa gaaagaaaat
    93181 cttctccttt tacgggacct ggataaacta catcagaagg gctatccaat ctttctcgga
    93241 gtgtcgcgca agcgatttgt catcaatatc cttgaagaaa gtgggtttga agtcaatcct
    93301 gagacagagc ttggtttccg aaatcgggac acggcttcgg ctcatgtaac tagtatcgct
    93361 gcgagacagg gtgtagaagt ggtgcgcgtg catgacgtag ctagtcacag gatggcagtt
    93421 gaaattgcct ctgccattcg tctggctgat gaagcggaaa atttagattt aaaacaatat
    93481 aaataagatg aaagaaattg aaaacaatca gtggattgct aactaccgga cggatcaacc
    93541 gcattttggc ttggaacgaa tggtggaact gttagctttg cgtggcaatc cccatcttaa
    93601 actcaaggtt ctccatatcg gagggactaa cggcaagggc tcgactattg cttttttgaa
    93661 aaagatgcta gaaaagctag ggctgagagt tggcgtgttt agttcgccct atctcattca
    93721 ttacacagac cagattagca tcaatgggga atcgatctca gaagcgaggc tagaagctct
    93781 catggcagac tatcagtctt tgctggaggg agaagcggtc gccaatttac agggcacaac
    93841 cgagtttgag attatcacag ccctagccta tgactacttt gcctcagagc aagtagatgt
    93901 ggctatcatg gaagttggca tgggtggact tttggatagt accaatgtct gtcagcccat
    93961 tttgacagga attacaacta ttggcttgga tcatgtggcc ctacttggtg acaccttgga
    94021 ggccatagca gagcagaagg caggtattat caaacaaggg atgcccttgg taacagggcg
    94081 tattgctcca gaagccttga ctgtgattga ccgcattgcg gaagggaaag atgcgccgag
    94141 acttgcctac gggacagatt atcaggttcg tcatcaagaa agtgtggtga caggggaagt
    94201 ctttgactat acaagtgctg tcagacaagg tcgcttccag actagcctgc ttggtttgta
    94261 ccaaatagag aatgctggga tggccatagc tttacttgat actttttgtc aagaagatgg
    94321 tcgagagcta gcaagcaatg actttcttgg tcaagccttg gaagaaacaa gttggccagg
    94381 gcgtttggaa atcgtgtcaa gagatccctt gatgattttg gatggagccc ataatcccca
    94441 tgctatcaag gccttgttgg taaccttgca agaacgtttt gcggattatc ataaggaaat
    94501 cctcttcact tgtatcaaaa ccaaagcctt gggggatatg ttggacttgc tgggagccat
    94561 gccagatacc gagcttactc taacacattt tgcggatagt cgggcgacgg atgaaagcgt
    94621 gctgaaagag gcagctaagt ctagaaatct cagctaccaa gattggcatg attttctaga
    94681 gcagaatttg acagataaaa aagaagagaa acaaacagtt aggattgtca caggttcctt
    94741 gtatttcttg agccaagtga gggcctatct gatggagagg aagaacgaga atggatacac
    94801 aaaagattga agcagctgta aaaatgatta tcgaggctgt aggagagaac gctaatcgcg
    94861 agggcttgca ggaaacacct gctcgtgtag cccgtatgta tcaagagatt ttttcaggtc
    94921 ttggtcaaac agcagaggaa catttgtcaa aatcctttga aattattgac gataatatgg
    94981 tggtagaaaa ggatatcttt ttccatacca tgtgtgaaca ccacttcttg ccattttatg
    95041 gtagagcgca cattgcctac attccagatg gtcgtgtggc aggcttgtct aagctagccc
    95101 gtacggttga agtttattcg aaaaaaccac aaattcaaga acgtttgaat atcgaagtgg
    95161 ccgatgcctt gatggactat ctaggtgcta aaggagcctt tgttgtcatt gaggcggaac
    95221 atatgtgtat gagtatgcgt ggtgttagaa aaccaggcac tgcaaccttg acgacagtag
    95281 ctcgtggtct atttgaaaca gataaggacc tccgcgacca agcttatcgt ttaatggggc
    95341 tataaaaaga atccgtttca agcggatttt tctagaaagg aatcattatg gatcaactgc
    95401 agattaagga tttggaaatg tttgcctatc atggtctttt tcctagtgag aaagaattgg
    95461 ggcagaaatt tgtcgtttca gccatcctat cctatgatat gaccaaggca gctacagact
    95521 tggatttaac agcctctgtc cattacggag aattgtgtca gcagtggacg acttggtttc
    95581 aggaaacgag tgaagatttg attgaaacgg tagcctataa actggtggaa cgtacctttg
    95641 agtcttatcc tcttgtccaa gaaatgaagt tggaactgaa aaaaccttgg gcaccggtgc
    95701 atttgtcact agatacttgc tcggtaacca ttcatcgccg caagcaacga gcctttatcg
    95761 ccctaggaag caatatggga gataaacaag caaacttgaa gcaagccatt gacaaactgc
    95821 gagctcgtgg catctatatt ctcaaagagt ccagtgtctt agcgacggag ccttggggtg
    95881 gagtggagca ggatagcttt gccaatcaag tggttgaggt ggaaacctgg ctaccagcac
    95941 aagacttgtt agaaaccttg ttagccattg agtcagagct gggacgggtg agagaagtgc
    96001 attggggacc tcgtttgatt gatttggact tgctctttgt ggaggaccag atcctttata
    96061 cagacgacct catattgcct catccttaca tagcggaacg cctttttgtc cttgagtcct
    96121 tacaggaaat tgcgcctcat tttatccatc cgatattaaa acaaccgatc cgtaacttgt
    96181 atgatgcttt gaaaaaatag aaaaactcta gttttcagtt acttgcaact gaaggctaga
    96241 gtttttaaaa taggtcattt tcttctggga ggaggatagt ttctctaccg tccatgtcta
    96301 aaaccagtac tcttggggga taacgagggt cgaaaggatg gttaaagtca aaatcaatgg
    96361 ctgtagggag gtgttgactt gaaaagtgga aggtaatctt tccttggtta ttaagcaatt
    96421 gaaactcgag ttcttcttcc aattcaaaga cattttttaa gaaatggtcg ataatatacc
    96481 aaaaagagtc aatgatgtca tcaggcaagc tggtaacaat gccaaaacta gcagaccgca
    96541 tgtgggtatt ggtaaaagcc atatctctgt ctcctttctt ttcccttatc atacagcaaa
    96601 taggattaaa aatcaagaaa aggtgatttt ttgaaaagga ttttaactac agggaaaaag
    96661 aaggaaaaaa attcccaaaa atctaccgaa gttaataggt aaattcccaa attaacttga
    96721 ttatataact ttcagttatt ttgagaagtt accgaaaaat attttttata tctattgact
    96781 tttaggggtg aaatttggta tgatagtagg cggtattgtt taccccattt gaaaggcccc
    96841 ggaaccttcc aaatactttt cgatgggaag gaacacccat caccgtaaac aaaaatcgaa
    96901 ctatatatag gagaaatcat gaacaaaaca acatttatgg ctaaaccagg ccaagttgaa
    96961 cgtaaatggt acgtagttga cgcaactgat gtaccacttg gacgtctttc tgcagtagtt
    97021 gctagcgtac ttcgcggaaa aaacaaacca acatttacac cacacactga tacaggtgac
    97081 ttcgtgattg ttatcaatgc tgaaaaagtt aaattgactg gtaaaaaagc aactgataaa
    97141 atctactaca ctcactcaaa ccacccaggt ggattgaaac aaatctctgc aggtgaactt
    97201 cgttctaaaa atgcagtacg tttgattgag aaatcagtta aaggtatgct tccacacaat
    97261 acacttggac gcgctcaagg tatgaagttg aaagtatttg ttggagctga gcacactcac
    97321 gctgcacaac aaccagaagt tcttgacatt tcaggactta tctaaggaaa ggaacaataa
    97381 agtatgtcac aagcacaata tgcaggtact ggacgtcgta aaaacgctgt tgcacgcgtt
    97441 cgccttgttc caggaactgg taaaatcact gttaacaaaa aagatgttga agagtacatc
    97501 ccacacgctg accttcgtct tgtcatcaac caaccattcg cagttacttc aactgtaggt
    97561 tcatacgacg ttttcgttaa cgttgtaggt ggtggatacg ctggtcaatc aggagctatc
    97621 cgtcacggta tcgctcgtgc ccttcttcaa gtagacccag acttccgcga ttcattgaaa
    97681 cgcgcaggac ttcttacacg tgactcacgt aaagttgaac gtaagaaacc aggtcttaag
    97741 aaagctcgta aagcatcaca atttagtaaa cgttaattcg aaagaattac tatacttata
    97801 cagagcacct ttcggggtgt tcttttttta tactttctta ctaaattggt gcaattgaca
    97861 cagttgttgc gactttagtc gcttacaaat gtggctgcaa cctgacaagg tcagttgcct
    97921 caaaacgtta atcaatacaa ttatatcaac gtttcaaagc actcaagagt ttacctcatg
    97981 ggtgcttttt ttatgttttt tgaaaaagtt cacctcaaag tttaccttat tttcacctta
    98041 ttgttttaga gactgtaagg caaattcacc gacggccata gggactacat tagaagtatt
    98101 gacataaatc tgtgttgtac ctgtgtcgct atgtattaga gcttcagaaa tggcttctaa
    98161 gctcattcct gcttgtttag cgagcgttgc tccggtatga cgtagtttgt gtggcgtagc
    98221 gtgtgtaagc tctgggtgtc gttttctgat agttttcatc ttattattga gatagtcaga
    98281 atgtaaaggt ttattgatat tgcctttggt atctatgtaa gtaaagatga attgttctgg
    98341 attactgatg ataccaaact ttgccagttc atatttttgt tgttttttcc atgaagtcag
    98401 aagttgaagt aagtcattag aaatagagaa aatgggaaat gtttccacaa taaaacgcat
    98461 attatcagat ggttaatgcg ttttatattt gggtttgagg cttataaaat aggataagcg
    98521 tactgaaaac ttagatatga caaacaaaat aaaattttta aaagctacat agaaacatgg
    98581 tataatgtat ctaaaggagg tagcatatgt ccttagatat agataaagaa aagatgacaa
    98641 tcatgggaat agcttttgaa aaccgatccg tttttaagag tgtttggtat gctttaagta
    98701 caaatatgat tgaaggatgg cgacctacgg ttagtgatgt tgaaaaatta cgagatgaag
    98761 ctcttgctct ggggatgact taatgaagcg caactgttat gaatcttatg actatgttga
    98821 tccggataat ttatataccg atccgggttc ttcagttcta agaaataagc aggaaggaaa
    98881 aaagcacgcg agcttgaata tcgtatgatt gctagtaaga gtttgaaatt atttatcaat
    98941 cctatcttag ttaatagttt aaaagtagtt tgtttcacaa ctgcataagc ttagaaaagt
    99001 tgaaaagtga taactcttta ataaactctt ggtttgttaa aagatatttt gtccactaca
    99061 tttttttcaa ttgactatta tttgtcatag atataacaga tagaacattg cattcagaat
    99121 aatgaaaaac gcatcaaaaa tggtgcgttc tgtttttatg ctaccataaa aggaggaaaa
    99181 caaattgaaa atttttaagg gagagtttta tcgaatctct gtattaacag acaagctagt
    99241 aaggttagaa tactctcgaa ctggaaattt tgaggataga acgacacaac ttatctataa
    99301 tagagatttt ggccaagttt cgttagatta tatcgagaca tcaaatgtac tagatattat
    99361 gacggactat tttcatctgc actttaataa aggagaattt aacgccgaaa atttatttat
    99421 agaattaaaa ggaaattttg ccgtatatgg tagtcgctgg tattttggtg aatctattga
    99481 aacgttaaaa ggaacagctc ggactctgga taaggcagat ggagcaatct cgttagaaga
    99541 tggaattatt agccgaaatg gtatagcctt attggatgat tctcaaggat ttatttggga
    99601 tgaacaatct ggttatattg agagagaaaa tcaaattgac ctgtatttct ttgcctatgg
    99661 gcatgattat agaggagcaa tcagagactt ttaccatttg actggttcaa cacccttgtt
    99721 gccaagatat gctttaggca attggtggag tagatattgg ccttatacgt cggatgaata
    99781 cttggattta atagacagat ttgaaacaga gaaaattcca ttatctatcg gtgtgttaga
    99841 tatggattgg catataactg acattccagc tcgttttgga agtggctgga caggatatag
    99901 ttggaataga aacttaatac caaatccaga acagttattg caacaacttc atgatagaaa
    99961 gttaaaactc tccttaaatg tccatcctgc tgatgggata cgggcttatg aagaagctta
   100021 tcctcaaatc gcaaaacgat tggggttaaa tgtagaacta gaagaacctg ctatttttga
   100081 tttttttaat ccctctttta gggaagccta ctttaaagat gttcattatg agttagaaaa
   100141 gcagggagta gatttttggt ggattgactg gcaacaaggg acacaaggta tgctagatcc
   100201 actttggctt ttaaaccatt atcactatca ggatagttgt aaaaatgcag aaggtggttt
   100261 gattttatca agatatgcag gtcctggtag tcaccgctac cctgttggtt tttcagggga
   100321 tactattatt agttggaatt ccttaagatt tcaaccctat tttacagcga cagcatctaa
   100381 tatcggttat agttggtgga gtcatgatat cggtggacat atgttggggg attatgacga
   100441 agagctacaa actagatggc tacagtttgg tgtttttagt ccgattactc gattacatag
   100501 ttctagaagt ccttttaata gtaaagaacc ttggtttttt tcagaaacaa catctaagat
   100561 tatgaagaaa tacctccgtt tgagacatca gatgattccc tatctatata ccatgaatgt
   100621 aaagacacat gaggaaggtg ccccattaat cagtccaatc tattatttct atccagagaa
   100681 tgatgagagc tataatgttc caaatcaata cttttttgga acagaactga tggtggctcc
   100741 cattgtagaa aagatggatt tgacattcca atctgcaaaa gtagatgtat ggttccctga
   100801 aggtgaatgg tatgactttt tttcagagaa aaaatacaca ggtggtgtga agttaagtgt
   100861 ttatagagac atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca ttcccttggt
   100921 tggttctgag ataggtatgg gtgttgattt acctgaagtt gtagattggt atgtattccc
   100981 aggaaaacaa cattcttttg aaatgcttga agatcaaaat ggtcaaagat ataaaacaag
   101041 attatcaatc gattgggaaa tgggaatggt agagttagca ttacaaggag attctagtat
   101101 cgttccaagc aatagaaaac atagaattca ttttaaagga acgaatgtgt ctataattga
   101161 attgccaaat aagaatgata cagctaaatt tgaatggaaa gataataaaa ggacatcttt
   101221 aaatgacgaa gtttttagac tactaaagac agcttctctt ccatatgaat taaaagatag
   101281 attgttaaat caattcatca atgcgaaaaa ctctcatgac ttaatgaata tcttgcatca
   101341 tcaggataag gaattgagag ggcgtttgtt ggaaatgata tttactagcc aaaactaatt
   101401 gaaataatta gaaagtcatt tgtttagtgt atatgaagat tctgtatggt attcgttatc
   101461 actggtcaca ttgaaaatgt gaaataagca cctacaacat agactttaca acacttcatg
   101521 ttttatatca tggggtgttt ttctatttaa gtcaaagccc atcaaaaaat ctcactattc
   101581 atcaggttaa aggaaaccta atcatagtga gattgtgaat tatagtagag tttgtatcgc
   101641 ctcttcaatt tgttttgggt ctgtttttga agaaaagcgt tcaaagactt gcccatctcg
   101701 accgatgaga aacttagaga aattccattc gattcgtttt cctagtgggc cggatttctg
   101761 gtcttttaac cagacataga gagggtctgc ttccttaccg ttgaccttaa tcttggcaaa
   101821 acgtgggaag gtggtttgaa aatgtaggct acagaaggcg ttgatttcct ctgcgctgcc
   101881 gggtgcttgt cccataaact gattgcaagg gaaatccaat atttcgaagc cctgagcttg
   101941 atagcgttca tagagttctt gaagtccctg gtactggggc gttaaaccac atccagtagc
   102001 agtgttgaca atcaagagaa ctttgccacg atagctatcc aagggagttg cttggttgtt
   102061 ttggttcaaa acggaaaaat cataaagtga agtcatgagg gcctcttttc tttttctaca
   102121 ttgtaacagt ttttactttt ttcgtcgagt cggatggaag atattgttga agaataagtc
   102181 ttcaatatga tagaaaccgt ttacaaataa ctttaaaaat ggtatgattt aattaaaccg
   102241 gtttacttag ttgctataaa tctctaattt gagatttagt tcctattgat tttacaatat
   102301 gtttattgga gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag tttgtcttca
   102361 cttgttttat caggattttt attaaaccat tatatgacag ttggagcgga agaaacgact
   102421 acgaatacca ttcagcaaag ccagaaggaa gttcagtatc agcaaaggga tacaaaaaat
   102481 ttagttgaaa atggtgattt tggtcagacg gaggacggaa gcagtccgtg gacaggaagc
   102541 aaagctcagg ggtggtcagc ttgggtagac cagaagaata gttctgcaga tgcctcaact
   102601 cgagtcattg aggctaaggg tggggctatc actatctcaa gccatgagaa attaagggca
   102661 gcggttcacc gtatggttcc tattgaagct aagaaaaagt ataaactgcg tttcaagatt
   102721 aaaacagatc ataaagtcgg gattgccaag gttcgtatca ttgaggaaag tggtaaggac
   102781 aagcgattgt ggaattctgc aacgacgtca ggaacaaagg actggcagac cattgaagca
   102841 gactatagcc cgactttaga tgttgataaa atcaagctgg agttattcta tgaaacagga
   102901 actgggactg tttcctttaa ggatattgag ctggtagagg tagcagacca gctttctgag
   102961 gattctcaaa cagataaaca gcttgaggaa aagattgatt taccaattgg aaaaaaacat
   103021 gttttttctc ttgcggacta tacttataag gtagaaaatc ctgacgttgc ttcagtcaaa
   103081 aatggaattt tagaacctct taaggaaggg acaaccaatg tcattgtcag taaagatggc
   103141 aaggaagtga aaaagattcc tttgaagatt ctagcctctg ttaaggatgc atacacagcc
   103201 cgtttggatg actggaatgg catcatcgct gggaatcaat actatgattc taaaaatgaa
   103261 cagatggcca aattaaacca ggaattggaa ggaaaggtag ctgatagcct atctagtatt
   103321 tcaagtcagg cggaccgcac ctatttgtgg gaaaaatttt caaattataa aatgtctgca
   103381 aatctgactg ccacttatcg gaaattggag gagatggcca agcaagtgac caatccttct
   103441 tctcgttatt atcaagatga aactgtcgtt cgaacagtca gggattccat ggaatggatg
   103501 cataaacatg tctacaatag tgaaaagagc attgttggga actggtggga ttatgaaatc
   103561 ggtacacctc gtgccatcaa caataccttg tctctgatga aagaatactt ctctgatgag
   103621 gaaattaaaa aatatacaga tgtgattgaa aaatttgtac cagatcccga acatttccga
   103681 aagacgactg ataacccatt taaggctcta ggtggaaact tagttgatat gggaagagta
   103741 aaagtaatag ctggtttact gcgtaaggat gatcaagaaa tttcttctac cattcgctcg
   103801 attgagcaag tgttcaagtt ggtagaccaa ggtgaaggtt tttatcaaga tggatcctat
   103861 atagaccata ccaatgttgc ctatacgggt gcttatggga atgttttgat tgatggcctg
   103921 tctcaactgt tgccagtcat tcaaaagacc aagaatccaa tcgataaaga taaaatgcaa
   103981 accatgtact actggattga taaatcgttt gctcctttgc tggtgaatgg agagctgatg
   104041 gatatgagtc gtggacgctc gatcagtcgt gcaaatagcg aggggcacgt ggccgcagta
   104101 gaagtactaa gagggattca ccgaatagcg gatatgtctg aaggagaaac caaacaacgt
   104161 ttgcagagtc ttgtgaagac cattgttcaa tcggatagtt attatgatgt ctttaagaat
   104221 ttgaagactt ataaggatat cagtttgatg caatccttgt taagtgatgc aggagtcgca
   104281 agtgttccaa gaccaagtta cctatctgcc tttaacaaga tggataaaac agccatgtac
   104341 aatgcagaga aagggtttgg atttggcttg tcactctttt ccagtcgtac cttgaattac
   104401 gaacacatga acaaggaaaa taaacgtggt tggtatacga gtgatgggat gttctatctt
   104461 tacaatggcg atttgagtca ctatagcgat ggctactggc caacagttaa tccatataag
   104521 atgcctggta caacagagac ggatgctaag agagcggata gcgatacagg taaagtttta
   104581 ccgtctgctt tcgttggaac gagcaaacta gatgatgcca atgcgacagc aaccatggat
   104641 ttcaccaact ggaatcaaac attgactgct cataagagct ggtttatgct aaaggataag
   104701 attgcctttt taggaagcaa tatccaaaac acttcaacag atactgctgc aactacaatc
   104761 gaccagagaa aactggaatc aagtaatcca tataaagtct atgtcaatga taaagaagcc
   104821 tcccttacag aacaagaaaa ggattatcct gaaacccaaa gtgtcttttt agaatcgtcc
   104881 gattcgaaaa agaatattgg ttactttttc tttaagaaga gttcaatcag catgagtaag
   104941 gctttgcaaa agggagcctg gaagggtatc aatgaaggac agtcagacaa ggaagttgaa
   105001 aatgaatttc ttacgattag tcaggctcat aagcaaaatg gagattctta tggctatatg
   105061 ctcattccta acgtggatcg tgccaccttc aatcaaatga taaaagagtt agaaagtagc
   105121 ctcatcgaaa ataacgaaac ccttcagtct gtttatgatg ctaaacaagg agtttggggc
   105181 attgtgaaat atgatgattc tgtctctact atttccaacc aattccaagt tttgaaacgt
   105241 ggagtctata ccattcgaaa agaaggggat gaatataaga ttgcctacta taatcctgaa
   105301 acccaggaat cagctccaga tcaggaagtc tttaaaaagc tagagcaagc agctcagcca
   105361 caagtacaga attcaaaaga aaaggaaaaa tctgaagagg aaaagaacca ttcggatcaa
   105421 aagaatctcc ctcagacagg agaaggtcag tcaatcttag caagtctagg gttcttgcta
   105481 cttggggcat tttatctatt ccgtagagga aagaacaact aatttgttca tcatctagat
   105541 gaattacatg aaactgtgga aaatagaaaa aatccgaaga aggccaaata tcttcggatt
   105601 tttcttttaa aaaaggataa gttgatactt atcctttttt acttcattat tatgtgacta
   105661 tagcgttttg aaactagaat agaacgttat aactgctaaa agatttctat aaattcattt
   105721 gattttccta atcaatttgt tcgtatccta tttcactcca ctatataagt ccgtctcgct
   105781 ccgtgaggtg cgcgtcgaag gttataccaa aaaaactcca aacgcgatac aataaaggta
   105841 ttcaatccaa ttgtaaagcg aaaggagaaa aaatatggca caaaagactc atagtttatc
   105901 atacacaaag tggatgtgtt ctatcatatt gtgttcaccc ctaagtatag acgaaaagtc
   105961 atctataatc aatatagaag tagtttaggc gaaatatttc atcgcttgtg tagttataaa
   106021 ggtgttgaaa tgatcgaggg tcacttaatg ccagaccatg tacacatgtt agtcagtatt
   106081 ccactaaggc taagtgtttc aagttttatg gggtatttaa aaggcaaaag tgcactcatg
   106141 atgtttgaca aacacgccaa tctcaagtac aagtttggga atcggcattt ctgggcggaa
   106201 ggttattatg taagtacagt agggcttaat gaagccaaaa ttaagaaata tagtcaagat
   106261 taaagaaatt atcgagtgga tgatttcttc acgagtatga aaatttgaga actagtaaag
   106321 tatgatatat agtaaaatga aataagaaca ggacgaaatc gatcaggaca gtcaaatcga
   106381 tttttaacaa tgttttagaa gtagaggtgt actattccag tttcaatcta ctatagcact
   106441 agataaattg agtgtaaaag aatatgagga tccctttagg gatagtggta agtaatacca
   106501 aagtctctta aagaggcaag tgacgagtca agagcaataa ggcttgaaca acgtgaaagc
   106561 cagcgtcttt aggcgctggc tgatgatttg ggcttatagc tctgagataa accacccgtt
   106621 agacgggtag ttatgatttt atctgagtgt aacatactgt tgggcaatct cgctgatgcg
   106681 gtcaaagttg ccttgggaag cgagtttatt gagttcgcca ccaattccaa cggcatctgc
   106741 accagcagcg aaccattgag ggatgttgtt tagaccgact cctccggtta ccattacgga
   106801 aacttgtgga atcggtgcct tgactgcaga gatatatgct ggactgagag tactacctgg
   106861 gaagagtttg atgatttcac taccggcttc aagtgcagtc gtgatctctg tgagggtaat
   106921 acagcctgga atgtacggtg tgctgtagag attgcacatt ttcgcagttt cagcatggaa
   106981 agatggagaa acaacgtaat ttgctccggc tagaatggca tctctagcag ttacggcatc
   107041 aagcacagta cctgcaccga tacaaacact ctgatcgtcc tgatacaagt ctacaagttc
   107101 cttgatgatt tgtcctgcat actgattggt ataggcgatt tcaatagctt tgataccgcc
   107161 cttgatacaa gcaatcgagg cttgtagtcc ttcttccttt gtatttcctc gaatgacagc
   107221 aacaattttc tgttttttta gttcaataat tgtatctgat ttggtcatgt aattctccta
   107281 acgaatgata tcttgtgcat ttgccagtaa attttcaata ctagttgcgg aagtggagag
   107341 atggtctcct ggaagagtgc atttgagagt tgcgctcgca actgcaaagt caatggtagt
   107401 ttttagggag gaatgatgga gtagttggta aagggcacca gatataaagg catccccgct
   107461 accaattcgt tgatagactg cagtttttag ttggacagac tcttcaaatc gttcttccag
   107521 agcataggct tgatagacat ttttgtcttg ctcatcacta gagcggaggg tatggaaaat
   107581 ggccttgaaa ccataggctt cttttaaaag tcgcatgcga ttttccacct cttctaggct
   107641 agcactgtct cttggaaaca tctctagatt ttggtcatca atcatgagag gatcaatacc
   107701 gaagcaatag tcagtaaaac gtgcaaactt agaaaattca tacttggctt ctaggactga
   107761 aatcatcttt gttctcagat tgagatccat tgaaacgaca attcctcggc gcttggcttc
   107821 ttccaagagt aggagaagga tcgcacggac ctcttgaccg atagctacgg tgattccact
   107881 aaaatgaaaa tggctaatcc cctgaaagag agaatccata tctagcatgt ttggccgaat
   107941 ctggctgata ctcgtatgct tacgatcgta gaaaacttca ctttgacgac aaccaaagcc
   108001 gttctccaaa tagtagaggc cgattcgatc gccaagccga cagattgaac tggtatcgat
   108061 ttggtgctgt ttcaagaatg tgagaaaacg atctccaatc tcgttggcag ggagtgcggt
   108121 aaagactttc gttgagatac ccagggcttg caaattacaa gcgatgttaa tttctgatcc
   108181 gccaaaataa gtcgaactgg caacatgatc tccgatactg gtggcatcta atggtgagat
   108241 tcgaattaat ggttcgccaa acagtaatat tttcgtcatc cattagttcc tttctggttt
   108301 agaatagctt tacttggcta atacttcttt caaataagca gcgacttcat catcttggca
   108361 gttttcaaag aagtattctt ggaagtattt gccagatatg gtgtctttga ggaagtcttg
   108421 gtcgattgtt ttaaggatgg tcatcaaatc attgtgggtg atttgtttca cctcgtttaa
   108481 gatacgagcg ttgcgttgtt cagggattac acgttctctt gggtagccac cgcccatttc
   108541 ttcagcaaat aagcgttcaa acatcaattt gagggtcagt tctgcccccc agccaaatcc
   108601 tttggcataa gggatagaca aggcgttacc accattgatt tgagaataaa ggtaagcgtc
   108661 agttgggtcc actgctagac cacagacaac accagggaag ctgtttaaag caagcatagc
   108721 ccctacaccc gtaccacagc cggtaacaac aaagtcaact gcctttgtat ttaaaaggat
   108781 ggcagccatt agtccgttct gcacataagt taattgactt tctccttctt ctccacgcat
   108841 accatagtta aataattggt agtctttttt atctgtcgct tcttttagac tatcgtaaat
   108901 aatgtgattc ttgctagctt gactattttc attgattaat gcgattttca taagatcttc
   108961 tttctatttt tactcaggtt gttttccgat gtaggctaag ataccgccat ctacatatag
   109021 gatgtggcca ttgacaaaat tgctggcatc actagcgaga aagacagcag ggcccatcaa
   109081 atcttcagga tttccccaac gtgcagcagg tgtttttgca atgatgaact ggtcaaatgg
   109141 gtggcgagaa ccatcttctt gcaactcacg aagaggtgct gtttgaggag tggcaatata
   109201 acccggtcca attccgttac attggatatt ggctccaccg tattcagacg caatgttgcg
   109261 ggtcaacatt ttcaagcccc ctttagcagc agcataagcg ctaactgttt cacgtcccag
   109321 ttcgctcatc atcgaacaaa tattgataat ctttccatgc cctttcttta tcatagaagg
   109381 aataactgcc tttgaaacga taaatggtgc gtttaagtca atatcgatga ccttacggaa
   109441 atcagcggcg ctcatttcgc acattggaac tcggcggata ataccagcgt tattaacgag
   109501 gatgtcaatg acaccaacct cttgttcgat ttgcttgacc atggcttgga taccgtcctc
   109561 gtctgtcacg tcacagacat atccatgggc ttcgatgcca acttcacgat aagcttcaat
   109621 ccctttattg accagatctt gattgatatc gttaaagaca atagtagcgc cggcttcagc
   109681 gtaggatttg gcaatagcaa atccaattcc ataagaagcg ccggtgatga gagcaatttt
   109741 tccttttaaa gaaaactgct caattgagaa tgatgtattt gtcatttgat acttcctttc
   109801 aaatttgtta agacttctct ataaccaaat tatattctag taaaccggtt ttgtcaataa
   109861 ataaatattt ttatttataa aaagatttat tttccttaaa aatggaatat tttgaaattt
   109921 ctgtctatta aaatggttaa aaagataatg aaagcgctaa tattttttta aaaaaactat
   109981 tgacaagata aaaataaagg attacaatgg ggaatataaa gtaaaccggt aaacctaaac
   110041 aagaaaggag aaaagatgaa aattgtactt gtagggcatg gacattttgc tacagggatt
   110101 tatagttctt tacaattgat tgcaggcaat caagaaagtg tggaggcgat tgactttgtg
   110161 gaaggaatgt cagcagatga actcaagcaa aaaatcttac ttgcaatttc aaatgaagaa
   110221 gaagttttaa tcctaagtga tctcttggga ggatcgccat tcaaggtttc ttctaccata
   110281 atgggagaaa atccagccaa aacaatgaat gttctctcgg gtttgaactt agccatgtta
   110341 atggaagcag tctttgctag aatggctcat agctttgatg aggttgttaa taaatcagta
   110401 gtggcggccc agggcggagt cgtaaatggt aaagaattgt tttcaacgga tgcagaggaa
   110461 gaggaagaag atttcgaatc gggtatttaa agggtaaaag aatgataaaa aaggttacga
   110521 ttgaaaaaat aaaatcgcct gagcgcttct tagaagtacc acttctgacg aaagaagaag
   110581 tcggccaggc aatcgataag gttattcggc agttagaact caaccttgac tatttcaagg
   110641 aagatttccc tacgccagct acctttgata atgtctatcc aatcatggat aacacggaat
   110701 ggaccaatgg tttctggaca ggagaactgt ggttggctta tgaatacagt caacaggatg
   110761 catttaaaaa catcgctcat aaaaatgttc tttctttcct ggatcgtgtc aataagagag
   110821 tagaattgga tcaccatgat ctcggcttct tgtacacacc gtcttgtatg gctgaatata
   110881 agataaatgg agatggagag gctagagaag caaccttgaa agctgcagat aagttgattg
   110941 aacgctatca agaaaaaggt ggttttattc aagcttgggg agacttgggc aagaaagagc
   111001 attaccgttt gattatcgac tgcttgctca atatccaact cttattcttt gcttatcaag
   111061 aaacaggcga tcaaaaatac tacgatattg cagaaagtca tttctatgct tcagctaata
   111121 atgtaatccg tgatgacgct tcgtccttcc acaccttcta ttttgatcct gagacaggtc
   111181 aaccctttaa aggtgtaacg agacaagggt atagtgatga ttcatgctgg gcacgtggtc
   111241 aatcatgggg agtctatggt attcctttga cttatcgtca cttaaaagac gagtcctgct
   111301 ttgacttgtt taagggtgtg accaattatt tcttgaatcg tctgccaaaa gatcatgtgt
   111361 cctattggga tttgattttt aatgatggta gtgatcaatc acgagattct tcagcaacag
   111421 ctatcgccgt ctgtgggatt catgaaatgc taaaacatct cccagaggtg gatgctgaca
   111481 aagatattta taaacatgct atgcatgcca tgcttcgttc cttgatcgaa cattatgcaa
   111541 atgatcaatt tacccctggt gggacaagtc tcctccacgg tgtgtactca tggcattcag
   111601 gtaaaggagt ggatgaaggc aatatctggg gtgactacta ttacctagaa gcccttatcc
   111661 gtttctacaa agactggaac ctatattggt aggaggagaa atatgacaat gccaaatatt
   111721 attatgaccc gtatcgatga acggttgatt catggacaag gacaactttg ggtaaaatac
   111781 ctaggttgta atacggtcat tgttgccaat gacgaagtaa gcacggacaa gatgcaacaa
   111841 actctgatga aaacagttgt gccagactca gttgccatgc gtttcttccc tttgcaaaag
   111901 gtgattgata tcattcacaa ggctaatcct gctcaaacga tctttatcgt tgtaaaggat
   111961 gtgaaggacg ctttaacctt ggtagaaggt ggtgtcccta tcaaagaaat caatattggg
   112021 aacattcaca atgctactgg taaagagcaa gtgacacgct ccatcttcct gggtgaagag
   112081 gacaaggcag ccctcaagga attaagccaa actcatcaag taacatttaa tacgaaaaca
   112141 actccaacag gaaatgatgg agctgttcaa gtcaacatta tggactatat ttaaaagagg
   112201 agatcgttat gtcgattaat gtatttcaag cgattttaat tggattatgg acagctttct
   112261 gttttagtgg aatgctatta ggaatttaca ccaatagatg tattgttctg tcatttggtg
   112321 tcggaattat tctaggtgat ctgcctactg ctcttgcaat gggagctatt ggtgaattgg
   112381 cttatatggg attcggtgtt ggtgctggag gtactgttcc accaaaccca atcggacctg
   112441 gtatctttgg taccttgatg gctatcacta gtgctggtaa agtcagtcca gaagcggctc
   112501 ttgccctctc tactccgatt gctgtggcga ttcaattctt acaaactttc gcctacactg
   112561 tacgtgctgg tgcgcctgaa acggcgatga agcacttgaa aaaccataat ttgaagaaat
   112621 tcaagttcac tctaaatgca acaatttggt tgtttgcctt tattggattt accttgggtt
   112681 gcttgggtgc cctttcaatg gataccttgt tgaaactcgt agactacatt ccaccggtat
   112741 tgcttacagg tttgacagtt gctggtaaaa tgctcccagc tatcggtttt gcgatgatct
   112801 tgtcagtgat ggctaagaaa gagttgattc cctttgtctt gttgggatat gtttgtgcag
   112861 cttatctaaa catcccaaca attggtattg caattgtagg tactatcttt gctttgattg
   112921 aattttataa caagccaaaa acagcggatc atgtggtaga ggaggaagca cacgatgact
   112981 ggatctaaca aattaacaaa acgtgattat cttaaaacgt ctttgcgggc attcttttgt
   113041 caaaatggat ttaactatag taactatcaa gggttgggat atgccaatgt gatgtatcct
   113101 gctttgaaaa aacactatgg agagaatcag gaaggtttct accaagcctt ggaagaaaac
   113161 tgtgaattct ataataccaa cccacacttc ctgcctttta ttaccagctt gcatcttgta
   113221 atgttggaaa atggccgtcc ggcaaaagaa acacgtagca tcaagatggc cttgatggga
   113281 ccattagcag gtattgggga ttctctctct caattctgtt tagctccatt gttctcaacc
   113341 atcgcagctt cgtttgctca agaaggcttg gttgtcggtc caatcttgtt cttccttgcg
   113401 atgaatacga ttttaacagc gattaaattg tcaactggtc tgtatggata caaactagga
   113461 acgactgtga ttgataaact aagcgagcag atggcaacga tttctcgtat tgccaatatt
   113521 attggtgtaa ccgtaattgc tggtttggca gcgacatctg ttaagattat ggtaccgatc
   113581 acctttgctg caggggaagt taaagcagac gctaaacaaa gtatcgtaag tattcaggga
   113641 atgcttgata aggttgctcc agctcttcta ccagccctat ttacactttt agtttactac
   113701 ttgatcaaag aaaagaaatg gacaacatat aaactcgtta ttttaacagt tatcatcgga
   113761 attatcggaa gctggcttaa gattatagct taatggatac aacattgttt tatggaatag
   113821 tgattgtctt ggcagtgagt ccccttttac tgtcaagctt tcattctatt cgtcagcaaa
   113881 agttgcttcg caaacagatg gagcaacgac aagagtattt agcttcttta acatctggtg
   113941 atgaagtgtt gttattgtct ggaattcatg gaaaaatcat ttctatccaa gatgacttgg
   114001 tctccttgca gattgcaaaa ggtgtggtca tctatgtaga aaaggaaagt gtaatgggaa
   114061 agacaaaaga actgcttttt aagtagttct ttttctggta atagataatg aaagaagaaa
   114121 gaagacaatt ttttgaaaga atcgatggaa accaatgtcg tgattatatc ttgtcccact
   114181 gttcaaaaga ctatgagaaa gtcaagagtt cccttgaacg cttgatggac aatcgtttta
   114241 tgtttgatag tccttgggat atggagcctt gttcaaaaat ccatcaaatc cagccgatgg
   114301 tatgggatca agtatttgaa gatgatccag aatggtctta tatgctcaat cgacaagaat
   114361 atctcttgca gtttatgata gggtatgtgg tagaaggaga taaggactat attcaaaagt
   114421 gcaagttctt tctatttgat tggattgagc aggtgagaga attttctcct caatccttga
   114481 tgactagaac cttggatacc ggtattcgtt cctttacttg gttgaaacta ctcttgctcc
   114541 tcttgaaatt tgacttgcta gaggagaaag aactagagaa aattttggtc agtctagaaa
   114601 agcagattga ctttatgaaa agccactatc gcgccaagta cacccttagt aactggggaa
   114661 ttttacaaac aattccgatg cttgctatct atcatttctt tgcagataag atggacctag
   114721 aagaagctta ccattttgct tcagaggagt tgaaacagca aattgagaca cagattttag
   114781 gagatggaag ccagtttgaa cagtcgattc tctatcatgt agaggtttat aaagccttgc
   114841 tggatttgtg tctcttgctt ccagacttgc aagatagtta ccaagagttg ctggaaaaga
   114901 tggcgaccta tattcaaatg atgacaggct tagatggacg aactttggct tttggtgata
   114961 gcgattctac agaaacgaca gaaatgttga gcctgtctgc tgtggttttg aacaaggaag
   115021 accttcttaa cggtctggat gttaaagtcg atttgcttag cctcttgttc ctggggcgag
   115081 aaaaggtcaa gcgactgcag gaatttgaaa agcgagcttg gcagcctaag ttcatgagct
   115141 ttgaagactc tggacatgtc tgcattaagg atgaacatcg ttatctattt ttcaaaaatg
   115201 gtccgctagg aagtgcccat agccatagtg acgagaatag tttttgctta cagtatcaag
   115261 gccaacctat tttcatagat gctgggcgtt attcttatcg ggagatatat gaacgttatc
   115321 tcttaaagag tgcttggagt cattcgacct gcattgtaga tgggaaagct ccggaaagaa
   115381 tcacgggatc ctgggaatat gaatactatc ctcactccct gttttgtcac cataaagaaa
   115441 gggagggaat gcattatatt gaaggggctt attggtcagc agaacctgat ttgccttatc
   115501 ttcacaggag aaaaatcctc atgttggtag aggatgtctg gctcttggta gatgacatca
   115561 ggtgtcaagg tcagcatgag gcgttgactc agtttatcct tgacaaggat gtgacctatc
   115621 aagatgggaa aatcaatcag ttgagactat ggagtgaagt tgattttgat ttggaagata
   115681 ccatcatttc tcctaaatac aatgagcttg aaagaagtag caaactcacc aagcgccaat
   115741 tctttgagaa tcagatgctg gattatacca tcattgcgca tgagagtttt gaaatcatcc
   115801 gtcattctgt ctaccagaca gatgatcgtg aagtggaaaa tgctctggct tttgaagtga
   115861 aaaatgacga aacagacaag ctgattctgt tattaagcga ggatatttgt gtaggtgaaa
   115921 aattgtgcct cgttgacgga acaaaaatgc gtggaaaatg tctagtatat gataaaataa
   115981 atgagagaat gattcgcttg cagtgctaga aataggcatt ttgaatagtg aatatgttat
   116041 aataagtatt agtaggaggt gttttagatt ggagaagaaa ctgaccataa aagacattgc
   116101 ggaaatggct cagacctcga aaacaaccgt gtcattttac ctaaacggga aatatgaaaa
   116161 aatgtcccaa gagacacgtg aaaagattga aaaagttatt catgaaacaa attacaaacc
   116221 gagcattgtt gcgcgtagct taaactccaa acgaacaaaa ttaatcggtg ttttgattgg
   116281 tgatattacc aacagtttct caaaccaaat tgttaaggga attgaggaca tcgccagcca
   116341 gaatggctac caggtaatga taggaaatag taattacagc caagagagtg aggaccggta
   116401 tattgaaagc atgcttctct tgggagtaga cggctttatt attcagccga cctctaattt
   116461 ccgaaaatat tctcgtatca tcgatgagaa aaagaagaaa atggtctttt ttgatagtca
   116521 gctctatgaa caccggacta gctgggttaa aaccaataac tatgatgccg tttatgacat
   116581 gacccagtcc tgtatcgaaa aaggttatga acattttctc ttgattacag cggatacgag
   116641 tcgtttgagt actcggattg agcgggcaag tggttttgtg gatgctttaa cagatgctaa
   116701 tatgcgtcac gccagtctaa ccattgaaga taagcatacg aatttggaac aaattaagga
   116761 atttttacaa aaagaaatcg atcccgatga aaaaactctg gtatttatcc ctaactgttg
   116821 ggccctacct ctagtcttta ccgttatcaa agagttgaat tataacttgc cacaagttgg
   116881 gttgattggt tttgacaata cggagtggac ttgcttttct tctccaagtg tttcgacgct
   116941 ggttcagccc tcctttgagg aaggacaaca ggctacaaag attttgattg atcagattga
   117001 aggccgcaat caagaagaaa ggcaacaagt cttggattgt agtgtgaatt ggaaagagtc
   117061 gactttctaa aatgaaggaa aatgacttgc aatctctgtt aagaaataaa ataatcccac
   117121 ctagaacaag ctaggtggga ttatttgcct atgaaatgag aaattatggg agcaagctcc
   117181 taaatcaact gtttttgatc tacttcttta actacttgat aaaagttata gaagtaggcc
   117241 aaacttgaaa tgatggttac gactaggaat attgaaaatt tccattggac agggttggtt
   117301 aaaagttgtg gaaaggatat gaggagaaag aagagggctg cgttgaggac aggtatccgt
   117361 tttgattgta ttttctcaag tcctttattg agcgcaggaa gaaagaggag taggagtagt
   117421 aaaactgtat gagaaacagc tcctgaaata agggcaaaga aaaggaaaat actgataaaa
   117481 acatgaatga tcagtagtct agctagtgat ttcataaggt acctcctaat cctggtcttt
   117541 tttagctctt gcaatacgaa gtgagtcgac aatatgtatc atcactccga aaaagaaagc
   117601 tcccagtata gttttaaaaa tatgttttgt atttagaaga gaactgataa aatttggatt
   117661 ttcacttgtt agggtatcaa tgagtggaat tataaaaaat atcactgttc cataaatcga
   117721 acctgctttc agaccaggat aacgtaactg tttcttttct tttttcatga gtttgctcct
   117781 tttttaaaaa tcttcctccc aaaagagact gttgaggtca gtttggaggc tgcgggcgag
   117841 attgagacag agttccaagg ttggattgta cttgtcgttt tcaatcatat tgatagtctg
   117901 tctcgagaca ccgatatcct tggcgagttc gagctgggaa atacccaatt ccttgcgaaa
   117961 ttctttcaca cgattcatct ggtctccttt ctgatttatg tcgtatatat ttgactatat
   118021 tatagtcttt taaacataaa gtgtcaagta tttttgacat attttttgaa gaaatagtag
   118081 tctccttgtc ctatttgtct gacaagtgca agctggtcgg atttgtggta aaatagataa
   118141 gatatgacaa aagaatttca tcatgtaacg gtcttactcc acgaaacgat tgatatgctt
   118201 gacgtaaagc ctgaaggtat ctacgttgat gcgactttgg gcggagcagg acatagcgag
   118261 tatttattaa gtaaattaag tgaaaaaggc catctctatg cctttgacca ggatcagaat
   118321 gccattgaca atgcgcaaaa acgcttggca ccttacattg agaagggaat ggtgaccttt
   118381 atcaaggata acttccgtca tttacaggca cgtttgcgcg aagctggtgt tcaggaaatt
   118441 gatggaattt gttatgactt gggagtgtct agtcctcaat tggaccagcg tgagcgtggt
   118501 ttttcttata aaaaggatgc gccactggac atgcggatga atcaggatgc tagtctgaca
   118561 gcctatgaag tggttaatca ttatgactat catgatttgg ttcgtatttt cttcaaatac
   118621 ggtgaggata aattctctaa acagattgcg cgtaagattg agcaagcgcg tgaagtgaag
   118681 ccgattgaga caacgactga gttagcagag attatcaagt tggtcaaacc tgccaaggaa
   118741 ctcaagaaga agggtcatcc tgctaagcag attttccagg ctattcgaat tgaagtcaat
   118801 gatgaactgg gagcggcaga tgagtccatc cagcaggcta tggatatgtt ggctctggat
   118861 ggtagaattt cagtgattac ctttcattcc ttagaagacc gcttgaccaa gcaattgttc
   118921 aaggaagctt caacagttga agttccaaaa ggcttgcctt tcatcccaga tgatctcaag
   118981 cccaagatgg aattggtgtc ccgtaagcca atcttgccaa gtgcggaaga gttagaagcc
   119041 aataaccgct cgcactcagc caagttgcgc gtggtcagaa aaattcacaa gtaagaggga
   119101 aaaagatggc agaaaaaatg gaaaaaacag gtcaaatact acagatgcaa cttaaacggt
   119161 tttcgcgtgt ggaaaaagct ttttactttt ccattgctgt aaccactctt attgtagcca
   119221 ttagtattat ttttatgcag accaagctct tgcaagtgca gaatgatttg acaaaaatca
   119281 atgcgcagat agaggaaaag aaaaccgaat tggacgatgc caagcaagag gtcaatgaac
   119341 tattacgtgc agaacgtttg aaagaaattg ccaattcaca cgatttgcaa ttaaacaatg
   119401 aaaatattag aatagcggag taagatatga agtggacaaa aagagtaatc cgttatgcga
   119461 ccaaaaatcg gaaatcgccg gctgaaaaca gacgcagagt tggaaaaagt ctgagtttat
   119521 tatctgtctt tgtttttgcc atttttttag tcaattttgc ggtcattatt gggacaggca
   119581 ctcgctttgg aacagattta gcgaaggaag ctaagaaggt tcatcaaacc acccgtacag
   119641 ttcctgccaa acgtgggact atttatgacc gaaatggagt cccgattgct gaggatgcaa
   119701 cctcttataa tgtctatgcg gtcattgatg agaactataa gtcagcaacg ggtaagattc
   119761 tttacgtaga aaaaacacaa tttaacaagg ttgcagaggt ctttcataag tatctggaca
   119821 tggaagaatc ctatgtaaga gagcaactct cgcaacctaa tctcaagcaa gtttcctttg
   119881 gagcaaaggg aaatgggatt acctatgcca atatgatgtc tatcaaaaaa gaattggaag
   119941 ctgcagaggt caaggggatt gattttacaa ccagtcccaa tcgtagttac ccaaacggac
   120001 aatttgcttc tagttttatc ggcctagctc agctccatga aaatgaagat ggaagcaaga
   120061 gcttgctggg aacctctgga atggagagtt ccttgaacag tattcttgca gggacagacg
   120121 gcattattac ctatgaaaag gatcgtctgg gtaatattgt acccggaaca gaacaagttt
   120181 cccaacgaac gatggacggt aaggatgttt atacaaccat ttccagcccc ctccagtcct
   120241 ttatggaaac ccagatggat gcttttcaag agaaggtaaa aggaaagtac atgacagcga
   120301 ctttggtcag tgctaaaaca ggggaaattc tggcaacaac gcaacgaccg acctttgatg
   120361 cagatacaaa agaaggcatt acagaggact ttgtttggcg tgatatcctt taccaaagta
   120421 actatgagcc aggttccact atgaaagtga tgatgttggc tgctgctatt gataataata
   120481 cctttccagg aggagaagtc tttaatagta gtgagttaaa aattgcagat gccacgattc
   120541 gagattggga cgttaatgaa ggattgactg gtggcagaat gatgactttt tctcaaggtt
   120601 ttgcacactc aagtaacgtt gggatgaccc tccttgagca aaagatggga gatgctacct
   120661 ggcttgatta tcttaatcgt tttaaatttg gtgttccgac ccgtttcggt ttgacggatg
   120721 agtatgctgg tcagcttcct gcggataata ttgtcaacat tgcgcaaagc tcatttggac
   120781 aagggatttc agtgacccag acgcaaatga ttcgtgcctt tacagctatt gctaatgacg
   120841 gtgtcatgct ggagcctaaa tttattagtg ccatttatga tccaaatgat caaactgctc
   120901 ggaaatctca aaaagaaatt gtgggaaatc ctgtttctaa agatgcagct agtctaactc
   120961 ggactaacat ggttttggta gggacggatc cggtttatgg aaccatgtat aaccacagca
   121021 caggcaagcc aactgtaact gttcctgggc aaaatgtagc cctcaagtct ggtacggctc
   121081 agattgctga cgagaaaaat ggtggttatc tagtcggggt aaccgactat attttctcgg
   121141 ctgtatcgat gagtccggct gaaaatcctg attttatctt gtatgtgacg gtccaacaac
   121201 ctgaacatta ttcaggtatt cagttgggag aatttgccaa tcctatcttg gagcgggctt
   121261 cagctatgaa agactctctc aatcttcaaa caacagctaa agctttggag caagtaagtc
   121321 aacaaagtcc ttatcctatg cctagtgtca aggatatttc acctggtgat ttagcagaag
   121381 aattgcgtcg caatcttgta caacccatcg ttgtgggaac aggaacgaag attaaaaaca
   121441 gttctgctga agaagggaag aatcttgccc cgaaccagca agtccttatc ttatctgata
   121501 aagtagagga agttccggat atgtatggtt ggacaaagga gactgctgag acccttgcta
   121561 agtggctcaa tatagaactt gaatttcaag gttcgggctc tactgtgcag aagcaagatg
   121621 ttcgtgctaa cacagctatc aaggacatta aaaaaattac attaacttta ggagactaat
   121681 atgtttattt ccctcagtgc tggaattgtg acatttttac taactttagt aggaattccg
   121741 gcctttatcc aattttatag aaaggcgcaa attacaggcc agcagatgca tgaggatgtc
   121801 aaacagcata aggcaaaagc tgggactcct acaatgggag gtttggtttt cttgattact
   121861 tctgttttgg ttgctttctt tttcgcccta tttagtagcc aattcagcaa taatgtggga
   121921 atgattttgt tcatcttggt cttgtatggc ttggtcggat ttttagatga ctttctcaag
   121981 gtctttcgta aaatcaatga ggggcttaat cctaagcaaa aattagctct tcagcttcta
   122041 ggtggagtta tcttctatct tttctatgag cgcggtggcg atatgctttc tgtctttggt
   122101 tatcaagtgc atctagggat tttctatatt gtcttcgctc ttttctggct agtcggtttt
   122161 tcaaacgcag taaacttgac agacggtgtt gacggtttag ctagtatttc cgttgtgatt
   122221 agtttgtctg cctatggagt tattgcctat gtgcaaggtc agatggatat tcttctagtg
   122281 attcttgcca tgattggtgg tttgctcggt ttcttcatct ttaaccataa gcctgccaag
   122341 gtctttatgg gtgatgtggg aagtttggcc ctaggtggga tgctggcagc tatctctatg
   122401 gctctccacc aagaatggac tctcttgatt atcggaattg tatatgtttt tgaaacaact
   122461 tctgttatga tgcaagtcag ttatttcaaa ctgacaggtg gtaaacgtat tttccgtatg
   122521 acgcctgtac atcaccattt tgagcttggg ggattgtctg gtaaaggaaa tccttggagc
   122581 gagtggaagg ttgacttctt cttttgggga gtgggacttc tagcaagtct cctgaccctc
   122641 gcaattttat atttgatgta agaatggcac cctgatgttt cagggtgttt ttgtgtttaa
   122701 atacacaatg aaaatcaaag aacaaactag aaagctaact ttaggctgct caaaatatag
   122761 cagattgaaa ctagaatagt acacatctac ttctaaaata ttgttagaaa tcgatttgac
   122821 tgtcctgatc gatttgtcct gttcttattt cattttaata tacagttttg aggttgtaga
   122881 taaggagaag ctgatgtggt ttgaagaggt tttcgaagag tgtaaaaaaa tattggtcag
   122941 ttaaagtcaa aactcttgac cttttctgac taatgtagta tattatgaac gtaaggtaaa
   123001 tgaaagcctt actagaacac ttatttaaag taaaatagaa agaggtgggt ctatgtttca
   123061 tctagaaatc ttcagaagta aagatagtct actcctgctt gaaaaagaaa aaccagaaat
   123121 agtacgtagg gtagcgatct agctagtcta aattttttaa aacaatggtc aaagatagtc
   123181 aatatcagta atcataacta agtaaacaaa aagaggtaaa gaatatgaat aacaacttta
   123241 ataattttaa taacatggat gatttattta accaattgat gggtggtatg cgaggataca
   123301 gttctgaaaa tcgccgttac ttaattaatg gacgcgaagt cacacctgag gaatttgctc
   123361 actatcgtac gactggtcaa ttaccaggaa atgcagaaac tgatgtgcaa atgccacaac
   123421 aggcatcagg tatgaaacaa gacggtgtcc ttgcaaaact aggtcgaaac ttgacagcag
   123481 aagcgcgtga gggcaagttg gatcctgtta tcggacgaaa caaggaaatt caagaaacat
   123541 ctgaaatcct ctcacgccgc accaagaaca atcctgtttt ggtcggagat gcaggtgttg
   123601 gtaagacagc agttgtcgaa ggtctagcgc aagccattgt gaacggagat gttcctgctg
   123661 ctatcaagaa caaggaaatt atttctattg atatctcagg tcttgaggct ggtactcaat
   123721 accgtggtag ctttgaagaa aatgtccaaa acttagtcaa tgaagtgaaa gaagcaggga
   123781 atattatcct cttctttgat gaaattcacc aaattcttgg tgctggtagc actggtggag
   123841 acagtggttc taaagggctt gcggatattc tcaagccagc tctctctcgt ggagaattga
   123901 cagtgattgg ggcaacaact caagacgaat accgtaacac catcttgaag aatgctgctc
   123961 ttgctcgtcg tttcaacgaa gtgaaggtca atgctccttc agcagagaat acttttaaaa
   124021 ttcttcaagg cattcgtgac ctctatcaac aacaccacaa tgtcatcttg ccagacgaag
   124081 tcttgaaagc agcggtggat tattctgttc aatacattcc tcaacgtagc ttgccagata
   124141 aggctattga ccttgtcgat gtaacggctg ctcacttggc ggctcaacat ccagtaacag
   124201 atgtgcatgc tgttgaacga gaaatcgaaa cggaaaaaga caagcaagaa aaagcagttg
   124261 aagcagaaga ttttgaagca gctctaaact ataaaacacg cattgcagaa ttggaaaaga
   124321 aaatcgaaaa ccacacagaa gatatgaaag tgactgcaag tgtcaacgat gtggctgaat
   124381 ctgtggaacg aatgacaggt atcccagtat cgcaaatggg agcttcagat atcgaacgtt
   124441 tgaaagatat ggctcatcgc ttgcaagaca aggtgattgg tcaagataag gccgtagaag
   124501 ctgtagctcg tgctatccgt cgtaaccgtg ctggttttga tgaaggaaat cgcccaatcg
   124561 gcagcttcct ctttgtaggg tctactgggg ttggtaagac ggagcttgct aagcaattgg
   124621 cactcgatat gtttggaacc caggatgcga ttatccgttt ggatatgtct gaatacagtg
   124681 accgcacagc tgtttctaag ctaattggta caacagcagg ctatgtgggt tatgatgaca
   124741 atagcaatac cttaacagaa cgtgttcgtc gcaatccata ctctatcatt ctcttggatg
   124801 aaattgaaaa ggctgatcct caagttatta cccttctcct ccaagttcta gatgatggtc
   124861 gtttgacaga tggtcaagga aatacagtaa acttcaagaa cactgtcatt attgcgacct
   124921 caaatgctgg atttggctat gaagccaact tgacagaaga tgcggataaa ccagaattga
   124981 tggaccgttt gaaacccttc ttccgtccag aattcctcaa ccgctttaat gcagtcatcg
   125041 agttttcaca cttgactaag gaagaccttt ctaagattgt agatttgatg ttggctgaag
   125101 ttaaccaaac cttggctaag aaagacattg acttggtagt cagtcaagcg gctaaagatt
   125161 atatcacaga agaaggttac gacgaagtca tgggggttcg tcctctccgt cgcgtggttg
   125221 aacaagaaat tcgtgataag gtgacagact tccacttgga tcatttagat gctaaacatc
   125281 tggaagcaga tatggaagat ggcgttttgg ttattcgtga gaaagcctaa gacagaattt
   125341 tgagaataaa aaagaaggag ccagctgaaa aaaactggtt ccttttgagg tacgacgggc
   125401 atgtcgtgta gtagagatgt actattctag tttcaatcta ctatagtagc tcagaaatcg
   125461 gtacttaaac gtgctatatc aaaaccagtc cttgaaaaac gtggactggt ttcgtgtttg
   125521 gattattact ttgaacgaca tgcgttaaaa gttagttgaa ccgccgtatg ccgaacggta
   125581 cgtacggtgg tgtgagaggg gctagagatt atcccctact cgattttaaa tcacatgacg
   125641 ttcaaaggca tcatctgaaa tcccttgttc caagatgagt tttgcccatt ctttagcaga
   125701 gaagaggctg tggtccttgt agtttccgca agattcgatg gttgtccctg ggacatcttc
   125761 ccaagtagta gtttcagcga tttccttgag cgaatccttg ataacagctg cgattttagc
   125821 actggtgtga cgtccccaca taatcatgtg gaagcctgtg cggcaaccaa atggcgaaca
   125881 gtcaatcatg ccgtcaatgc gggtacggat gagtttggct aagaggtgct cgatagtgtg
   125941 aaggccggcg gtagggatag agtcttcgtt tggttgcacc aagcgaatat cataattgga
   126001 gatgatgtct ccttttggtc ctgtttcttc cccaatcaag cgaacatagg gtgctttgac
   126061 aatggtgtgg tcaagttcaa aactttcgac aataacttct tttgacatgg taaatccttt
   126121 cagttttctt ctctcattat atcataaagg ttgctcctga gacagagaga aaacctctcc
   126181 gaggctggag aggttgaaat ctttacttac gatataagcg gtcgtattgg tagtatgggt
   126241 caaaggttac gttgataccc agtttacgaa ggacattctt gtcttcatca gtcaagatga
   126301 tggttgagtg ggcttcgctt cctttgaggt tgccgagttc ttccatagcg cgggtagcat
   126361 caggattttc tgtagctgtg atagcaagtg caatcaggat ttcatttgaa tgaaggcgtg
   126421 gattgcggct accgagatga tcgattttaa gaccttggat tggcttaaca acttccggct
   126481 cgattaattt tacttcttta gcgatgtcag ctgatttttt gatggcgttg atcaaggcag
   126541 cggctgtagg gccaaagagt tctgagttct taccagtgac gatttcccca tttggcaatt
   126601 caaaggctag ggctggtcca ccagtttctt ctgctttttg gcgcgcaacg acagcaacct
   126661 tacggtctgc aggtgtgata ccgaggtcgt tcatgagcaa ctcaattttc ttgacggcag
   126721 cttcgccaac tttttcagct ttgaagtcaa gaactgtttg atagtaacgg cggatgattt
   126781 cttgtttaga agcttcgaca gcggcctcgt catctgtaat agcgaaacca accatgttga
   126841 cacccatatc tgtcggtgaa gcgtatggtg attttccgag aatacgttcc aacatgcgtt
   126901 tgagcactgg gaagatttcg atatcacggt tgtagttgac agtggtttct ccataggttt
   126961 ggagatggaa ggggtcaatc atgttgacat catcaaggtc agctgtggca gcttcataag
   127021 ccaagttaac tggatgatga aggggaagat tccaaacagg gaaggtttca aatttagcgt
   127081 agccagattt gatgccattg atttggtcgt ggtacatatt ggacatacac gttgccaatt
   127141 ttccagaacc aggtccagga gcggttacga caatcaagtt gcgactggtt ttgatgtagt
   127201 cgtttttgcc catgccttct ggggaaatga tgtgatccat atccgtcgga tatcctttga
   127261 ttggataatg aagatatgaa tcaattccgt ttttctcaag ttggttgcgg aaggcatctg
   127321 cagcgggttg gccagcgtat tgtgtaatga caacggaacc aacaaaaatc cccaattcat
   127381 tgaatttgtc aatcaaacga agaacttctt ggtcataaga aatgcctaag tcgccacgtg
   127441 ctttggaatg ttcaatgttg ctagcattaa tggcaatcac aacctcaacc tgctctttca
   127501 attcttgcaa gagcttgatt ttgttgtcag gttcataacc aggaaggaca cgagcagcgt
   127561 ggaaatcttc taacatttta ccgccaaact ctaagtagag cttgccgtca aattggttaa
   127621 tacgctccaa aatatggtcg cgttgtaaat tcaaatattg ttcagaacta aaagcttgtt
   127681 ttttcatttt tttacctctg gactctatta taataaaaaa ttggaagtta ggaaactacg
   127741 gagctaaaaa agaaattaaa aagattaagc aaacgcttgc acaaaatttt aaaaagtgct
   127801 atcatagact atagattatt aaaataatga ggtaaacaga tgcaagaaaa atggtggcat
   127861 aatgccgtag tctatcaagt ctatccaaag agttttatgg atagtaatgg agatggagtt
   127921 ggtgatttgc caggtattac cagtaagttg gactatctag ccaagctagg aatcacagca
   127981 atttggcttt ctcccgttta tgacagccct atggatgata atggttacga tattgctgat
   128041 tatcaagcga ttgcaacgat ttttggaacc atggaggata tggatcaact gattgcagaa
   128101 gctaagaagc gtgatatccg tatcatcatg gacttggtgg tcaatcatac ctcggatgag
   128161 catgcctggt ttgtagaggc ctgtgaaaat cctaatagcc ctgagcgaga ctactatatc
   128221 tggcgcgatg aacccaatga cctagattct atctttagtg ggtctgcttg ggaatacgat
   128281 gaaaagtcag gtcaatacta tctccacttt ttcagcaaga aacagccgga tctcaactgg
   128341 gaaaatgaaa aacttcgcca gaaaatttat gagatgatga acttctggat tgataaaggt
   128401 attggtggtt tccgtatgga tgttattgac atgattggca aaattcctga cgagaaggta
   128461 gtcaataatg gtcctatgct ccatccctat ctcaaggaaa tgaatcaggc gacctttgga
   128521 gataaggatc tcttgacagt aggggagact tggggagcaa cgccagagat tgccaagctc
   128581 tactctgatc caaaggggca agaattgtct atggtcttcc agtttgaaca tatcggtctt
   128641 cagtatcagg aaggtcagcc taaatggcac tatcaaaaag agctgaatat cgctaagtta
   128701 aaagaaatct tcaacaaatg gcagacagag ttaggagttg aggacggctg gaattccctc
   128761 ttctggaaca accatgacct ccctcgtatt gtctcaatat ggggaaatga ccaagaatac
   128821 cgcgaaaaat ctgccaaagc ctttgcaatc ttgcttcatc ttatgagagg aactccttat
   128881 atctaccaag gtgaggagat tgggatgacc aactatccgt ttgaaacact agatcaagta
   128941 gaagatattg aatctctcaa ctatgcgcgt gaggctcttg aaaaaggtgt tccgattgaa
   129001 gaaatcatgg acagtatccg tgttattgga cgtgacaatg cccgtacccc tatgcaatgg
   129061 gacgagagca aaaacgctgg tttctcaaca ggtcaacctt ggttggcggt taatccaaat
   129121 tacgagatga tcaacgtcca agaagcgctg gcaaatccag attctatttt ctatacctat
   129181 cagaaactgg tccaaattcg caaggagaat agctggctag ttcgagctga ctttgaattg
   129241 cttgatacgg ctgataaggt ctttgcttat atacgtaagg atggcgaccg tcgcttccta
   129301 gttgtggcta acttgtccaa tgaagagcaa gacttgacag tagaaggaaa agtcaaatct
   129361 gtcttgattg aaaacaccct agctcaagaa gtctttgaaa aacaaatctt agttccatgg
   129421 gatgctttct gtgtggaaat gactgactag aatgagcaaa cctcaagttt ttgaagcttg
   129481 aggtttttta ctatagtgga ttgaaactag aatagtgcac ctctgcttct aaaacattgt
   129541 tagaaatcga tttgactgtc ccgatcgatt tgtcctattc ttatttcatt ttgctatact
   129601 tgtagttgag gagaattaag ctcctcacta gtaaactctt cataatcctt tttattttta
   129661 tgaagatatt gtttgaaaga tgtgagtttc cacggatggg tttgtggagg gatatacttg
   129721 cgtctttctt tttttgttct ggttcttgtt caaagttttt cgaatagagt tcatgattta
   129781 gtagctcctt tgtgtgatag attttgtcag cgatattgag gtagatgtct ccgtcaaatg
   129841 cttttataac taatgctttc gtctttctga tgaaatagac ttcttttcct tgttcggtag
   129901 ggatatagta acgattttgg aatcggatat ggtgtccact atcgacgact ctctccgcca
   129961 gtctagccag aatgagattc ctttcagagg ggttaggaac ctcctcgaag acagagagtt
   130021 ttgtcttgtt tccaaactgt tcattaaagg tttgaatgta ggaaagcagg aaggtattgg
   130081 cttcttccaa ggtatgaata ttgttttgtt ccagttcgat aggcaggcga gattgtagtg
   130141 tctgattgag tctttcgact ctccctttag cttgagggat agaggttgtc tcaaggagaa
   130201 tccctagttg gtgacaggcg tatccaaact gtgtatgggt atcgtcctcc attttcttag
   130261 agttggaggc ttgataggta aagaccgttc tcttatctgt tttaatttga aggggaatgc
   130321 cgtgattggc taagatttgt tcgaggacat gatagtaagc attcaagttc tcttgtttat
   130381 caaaataagc gcctaggata ttgccagaag catcatcaat ggctaagtgt aagttagatg
   130441 tttgggctcc aaactaggca tgagggctgg catccatttg aatgagttca ccagcaaatt
   130501 tctttctggg tctactagga tgtacttttt tagggtcttc aaggaagttt tcagccgtcg
   130561 gtaagattgg attgtctagg ggttgattca ggttcagttt agcttgtttt cttactctct
   130621 tctttgtctt tctatgggac ttaggcgaca ggatattttt cttatagagt atttttctaa
   130681 cagtagtatc agagagctga attccttctt cttcagctag caattcacag aaatgaagga
   130741 catttggttt atatgtttca taggagaggt atttttttag gagacgttct ttgatttcat
   130801 cagggattgc atgttttggt tttcgatttc tgtttccgtg tctgaaggct tcttttcctt
   130861 tcagttgata ggctagtagc agacgattga tttgtctttc agaaagattg agctcgacac
   130921 aggctcgttt ctttgttttc tttccttggg ctatagcttt tatcacaaga tattttttcg
   130981 tttcattcat attcagttgg atccttttca tatgactatt ctaccaaatg ggacattttc
   131041 acgttcgatt tactaaagac attatcacat tcgaattaca caagatgcag atagtgaaaa
   131101 taaaggtgta gacattaccg taaaaaagtg atataattgt atgatgttca atgtataggt
   131161 gttaatcatg agtagacgtt ttaaaaaatc aggttcacag aaagtgaagc gaagtgttaa
   131221 tatcgttttg ctgactattt atttattgtt agtttgtttt ttattgttct taatctttaa
   131281 gtacaatatc cttgctttta gatatcttaa tctagtggta actgcgttag tcctactagt
   131341 tgccttagta gggctactct tgattatcta taaaaaagct gaaaagttta ctatttttct
   131401 gttggtgttc tctatccttg tcagctctgt gtcgcttttt gcagtacagc agtttgttgg
   131461 actgaccaat cgtttaaatg cgacttcgaa ttactcagaa tattcaatca gtgtcgctgt
   131521 tttagcagat agtgagatcg aaaatgttac gcaactgacg agtgtgacag caccgactgg
   131581 gactgataat gaaaatattc aaaaactact agctgatatc aagtcaagtc agaataccga
   131641 tttgacggtc aaccagagtt cgtcttactt ggcagcttac aagagtttga ttgcagggga
   131701 gactaaggcc attgtcctaa atagtgtctt tgaaaatatc atcgagtcag agtatccaga
   131761 ctacgcatcg aagataaaaa agatttatac caagggattc actaaaaaag tagaagctcc
   131821 taagacgtct aagaatcagt ctttcaatat ctatgttagt ggaattgaca cctatggtcc
   131881 tattagttcg gtgtcgcgat cagatgtcaa tatcctgatg actgtcaatc gagataccaa
   131941 gaaaatcctc ttgaccacaa cgccacgtga tgcctatgta ccaatcgcag atggtggaaa
   132001 taatcaaaaa gataaattaa cccatgcggg catttatgga gttgattcgt ccattcatac
   132061 cctagagaac ctttatggtg tagatattaa ttattatgtt cgtttgaatt ttacctcatt
   132121 tttgaaattg attgacttat tgggaggggt agatgttcat aatgatcaag agttttcagc
   132181 tctacatagg aagttccttt tcccagtagg gaatgtccat ctagactctg agcaggctct
   132241 aggttttgta cgtgaacgct actcactagc cgatggagac cgtgaccgtg gtcgcaacca
   132301 acaaaaggtg attgtggcta ttcttcaaaa attaacgtca accgaagcac tgaaaaatta
   132361 tagtacgatc attaatagct tgcaagattc tatccaaaca aatatgccac ttgagactat
   132421 gataaatttg gtcaatgctc agttagaaag tggtggaact tacaaagtga attctcaaga
   132481 tttaaaaggg acagatcgga tggatcttcc ttcttatgca atgcctgaca gtaacctcta
   132541 tgtgatggaa atagatgata gtagtttagc tgtagttaaa gcagctatac aggatgtgat
   132601 ggagggtaga tgaaatgata gacatccatt cgcatatcgt ttttgatgta gatgacggtc
   132661 ccaagtcaat agaggaaagc aaggcacttt taagagaagc ttataatcaa ggagttcgaa
   132721 tgattgtgtc tacttcgcat cgtcgaaaag ggatgtttga aactccggaa gagaagatag
   132781 cagaaaactt tcttcaggtt cgggaaattg caaaagaagt agcagatgat ttagtcatag
   132841 cttatggcgc agagatatac tatactctgg atgctctaga aaagctagaa aaaaaagaaa
   132901 ttcctaccct taatgatagt cgttatgctt tgattgagtt tagcatgcat acttcctatc
   132961 gtgagattca tgcgggattg agcaatattt tgatgttggg aatcacgcca gtaattgctc
   133021 atattgaacg ttatgatgcc ttagagaata acgaaaaacg tgttcgtgaa ctgattgata
   133081 tggggtgcta tactcagata aatagttatc atgttttaaa acctaagttc tttggtgaaa
   133141 aatataaatt catgaaaaag agagctcggt attttttgga acgtgattta gttcatgtag
   133201 ttgcaagtga catgcacaat ttagacagta gacctccata tatgcaacag gcatatgata
   133261 tcattgctaa gaaatatgga gcgaaaaaag cgaaagaatt ttttgtagat aatcccagaa
   133321 aaattataat ggatcaatta atttaggaga aaatatgaag gaacaaaaca ctttggaaat
   133381 cgatgtattg caactattca gggctttatg gaaaagaaag ttggtcattt tattagtggc
   133441 aattataact tcttcagttg cttttgccta cagtactttt gttatcaaac ctgagtttac
   133501 tagtacgact cggatttatg tagttaaccg taatcaggaa gagaagtctg gtttaaccaa
   133561 tcaagacttg caggcaggat catacttggt taaagactat cgtgaaatta tcctatcgca
   133621 ggatgttttg gaggaagtta tttctgattt gaaactagat ttgacgccaa aaggtttggc
   133681 taataaaatt aaagtaacag taccagttga tacccgtatt gtctctgtct cagttaatga
   133741 tcgagttcct gaagaggcaa gccgtatcgc taactctttg agagaagtag ctgctcaaaa
   133801 aattatcagt attactcgtg tttctgatgt gacaacactg gaggaggcaa gaccggcgat
   133861 atcaccatct tcgccaaata ttaaacgcaa tacactaatt ggttttttgg caggggggat
   133921 tggaactagt gttatagttc ttcttcttga acttttggac actcatgtga aacgtccgga
   133981 agatatcgaa gatacactgc agatgacact tttgggagtt gtaccaaact tgagtaagtt
   134041 gaaataggag agaggaatgc cgacattaga aatagcacaa aaaaaactgg agttcattaa
   134101 gaaggcagaa gaatattaca atgccttgtg tacaaatata cagttgagcg gagataaact
   134161 aaaagtaatt tccgttactt ctgttaaccc tggggaagga aaaacaacta cttccgtaaa
   134221 tatagcaagg tcgtttgcgc gtacaggcta taaaactctt ttgatcgatg gcgatactcg
   134281 aaattcagtt atatcaggag tttttaaatc gcgtgaaaaa attacagggc taacagaatt
   134341 tttatctggg acagctgatt tatctcacgg tttatgtgat acaaatattg aaaatttatt
   134401 tgtaattcaa tcgggatctg tatcaccaaa ccctacagcc ttgttacaaa gtaaaaattt
   134461 taatgatatg attgaaacat tgcgtaaata ttttgattat atcattgttg atacagcacc
   134521 tattggaatt gttattgatg cggcaattat cactcaaaag tgtgatgcgt ccatcttggt
   134581 aacagcaata ggtgaggtga ataaacgtga tgtccaaaaa gcgaaacaac aattagaaca
   134641 aacagagaaa ctgttcctag gagttgtttt aaataaattg gatatctcgg ttgataagta
   134701 tggagtttac ggttcctatg gaaattatgg taaaaaataa cttaggaaag attttatgga
   134761 tgaaaaagga ttgaaaattt ttctagcagt aatacagagt attattgtca ttttattggt
   134821 ttattttctt agctttgtta gagagacaga acttgaacgt tcttcgatgg ttatactata
   134881 ccttctccac ttttttgtat tctattttag ttcctatggt aacaattttt ttaaaagagg
   134941 gtacctagtt gagtttaata gtactataag atatattttt ttctttgcaa tagctataag
   135001 tgtattaaac ttttttatag cggaacggtt tagtatctct agaagaggaa tggtatactt
   135061 cttaacttta gaaggaatat ccttatactt gttaaatttc ttagtaaaga aatattggaa
   135121 gcatgtgttt tttaatctaa aaaatagcaa gaaaatttta ctgttaacag taacgaaaaa
   135181 tatggaaaaa gttcttgata aattgctaga atctgatgaa ctttcatgga aattggtagc
   135241 agtaagtgtt ttggataaat ctgattttca acatgataaa atacctgtaa ttgaaaagga
   135301 aaaaattatt gaatttgcaa cgcatgaagt tgtggatgag gtgtttgtca atcttccagg
   135361 agagagctac gatattggag aaattatctc taggtttgag acaatgggga tagatgtaac
   135421 tgtaaatctt aaagcatttg ataagaattt gggtcgcaat aaacaaattc atgagatggt
   135481 aggattgaat gtagtcactt tctctacaaa tttttataaa actagtcatg tgatttcaaa
   135541 gagaattctc gatatttgtg gtgccactat tggccttatt ctttttgcta tagctagtct
   135601 agttttagtt ccattgattc gtaaagatgg cggatcagct atttttgctc aaactcgtat
   135661 agggaaaaat ggtcgacatt ttacctttta taaattccgt tcgatgcgaa tcgatgctga
   135721 agctatcaaa gaacagttga tggatcaaaa tacgatgcaa ggtggtatgt ttaagataga
   135781 caatgatcct cgtgttacaa aaattggtcg ctttattcgt aaaactagtt tagatgaatt
   135841 gccgcagttt tggaatgtct ttataggaga tatgagtttg gtgggaacac gtccacctac
   135901 agtagacgag tatgatcagt atactccaga acagaaacgt cgactcagct ttaaacctgg
   135961 tattacaggt ttatggcagg ttagcggccg tagtaaaata accgattttg acgatgttgt
   136021 aaaattagat gtggcttata ttgataattg gacaatctgg aaagatattg aaattttgct
   136081 taaaactgtt aaagttgtat ttatgagaga tggagcgaag taattcatat gaatgagaga
   136141 atgcaaatat taggagttac tatagatcca ttaacgatga aagaaacagt ggatgctgtt
   136201 gaacagtatg ttttaaagaa gcatcctttg cacttgatgg gagttaacgc agataaaatt
   136261 aatcagtgcc atgaagatga gaaaatcaaa aaaatcgtta atgaatcagg aatcattaat
   136321 gcggatggag catcagttgt tcttgcaagt aagtttttag gaacgcctgt tcctgaacga
   136381 gtagcgggta ttgatttgat gcaatgtctt ttagagttgt caaataaaaa aggatattca
   136441 gtttactttt ttggagctaa agaagaaatt ttacaagata tgctaaaagt ttttaaacaa
   136501 agatatccta atcttaatgt tgttggctat agaaacggat atttttcacc tgaagatgaa
   136561 aaaaagattc aggaagatat taaaggagga aaaccagatt ttgtatttgt agggattact
   136621 tctcctaaaa aggagtatat catccaatca ttcatggata atgggattaa tgctgtcttt
   136681 atgggtgttg gtggcagttt tgatgtatta tcaggacaca ttaaacgagc tccattatgg
   136741 atgcaaaaat tgaatctgga atggttgttc cgtgtagtta atgaacccaa acgccttttt
   136801 aagcgttatt ttatagggaa tgttactttt attaagcgag ttttagatga aaagcgaaaa
   136861 tcaaaaaaat aatattctac atatttctcg tacaatggat attggtggtg cagaacgtat
   136921 tgtatatcaa ttgagttctg atttgaaaga tgaatttgat agtgtccatg tcgcttcaac
   136981 aggtggacta tgggagagca aacttttatc aaaaggagtt caacatcata aaattttaga
   137041 tgttgacagt aagaatcccc taactatggt aaagatcctt tcaagttttt atagaattat
   137101 caaaaataat gatattacca ttgttcacac tcatcatcgg atggctgcct tttatattcg
   137161 cttgttgaaa atcattaatc ctaagttgat acatgtttac actgcacata atgtatttaa
   137221 aaataaatta ccattataca aattttcctt aaaaaaagcg tattgtgtag ctgtaagtaa
   137281 agcggttaat gataatttga aacatgatgt aggaatcagt gatagtacag taatctataa
   137341 tggggttgtt tttgaaaaaa ctgataagct agttggtgaa attgtaaact ataacggaat
   137401 caaattgggg tgtattgcta ggttatcaga gcaaaaagga ttgacctacc taattgatgc
   137461 gatgtcttta gtgaaacaca ggaatattac gttgttcatt gtcggagatg gagagcttcg
   137521 agatgagctt gaaaacaagg tgaaacaatt aaatcttcaa aaatcagtga tatttttagg
   137581 ttatcgaaaa gatattgttg aatgtatcaa tagttttgat tacttagtgt catcatcttt
   137641 atatgagggg tttggtctaa atgcaattga agcatttatg aaggggaaaa cgatggtttc
   137701 atcagatatc ccaggaataa atgaagtagt caataacaaa aatgggattc ttgttcctgt
   137761 taaggatgat gttgccttag cgagagcgat tgaaaagctt gcgactgaca aaaaacttag
   137821 agaaaaactc gcttatcaag caaaaaaaga ttatgagaca aaatttaatt attccttgtt
   137881 tttggataat tatcggagac tttatagaaa attaatggga gaatcaaaat gaaaaaagtc
   137941 atgttggttt ttgggacacg tccagaagct attaaaatgt gtcctttagt aaatgaactg
   138001 aaacaaaatg attcaatcaa gacacttgtg tgtgtaactg gtcaacataa ggaaatgtta
   138061 gagcaggttt tagaggtgtt caaggttgtc ccagattatg atttgggtat catgaaagca
   138121 aatcagaccc tatttaccat tacgactagt atcttggata aaattcaggc ggttctagag
   138181 aaagaaaaac cagatcttgt tcttgttcat ggagatacta caacaacatt tgcgacagct
   138241 ttagcagcat tttatatggg aatcaaagtg ggacacgtcg aagcaggtct cagaacctat
   138301 aatctccaaa gtccatttcc agaggaattc aatcgccaga caacttctat cattgccgac
   138361 tatcattttg cgccgactga agttgcgaag gaaaatctac taagagagga cagaaaaaat
   138421 atctttgtga cgggaaatac agtcattgat gctttgaaga cgactgttca agataactac
   138481 catcacccaa ttttagaatg ggcaaagggt agtaagttaa tcatgctaac ggctcatcga
   138541 cgagaaaatc ttgggaaacc gatggaaaac atgtttaatg ctgttaatcg aattttaaac
   138601 gaattcgaag atgtaaaagt tgtatatccc atccataaaa atcctaaggt tcgagaatta
   138661 gcaagtaaag tttttggcga taatgaacga atgaaaatca ttgaaccgtt agaggttatt
   138721 gattttcata attttatgaa tcaaagctat atgattttga cagattctgg aggtgtacag
   138781 gaagaagcac cttctttagg aaaacctgtc ttggtgatgc gtgatacaac agagcgtcct
   138841 gagggggtcg ttgctggcac gttgaagttg gtaggaacag aagaagagaa tatctatagc
   138901 aacttcaaac ttcttcttga agatgaaaca gaatataaca agatgagcaa agctagcaac
   138961 ccttatggag atggaatgac ttgtaaacgg attgttgata ttattctgga agtataaaaa
   139021 taattcaatt atggatgagg aaagaataaa ttagattgat gataaaaaga aaaatatata
   139081 taatctgtgt attactgtcg tattttttac ctagtttttt tacagttgga tcaatggcga
   139141 ctttatttgc tttgctttct actttagtta agctatttgt aacttttgat tatattccca
   139201 aagttctttt acagcataaa gtaacactga ttgatattct agttgttcta tttttatttt
   139261 ttcaagcatt tgcagctttt caaagccaaa cgttgtacct taactatgtt ggaggtcagt
   139321 ttttcctttt aggactatat tcatttttga aacacttttt attgttggat tgtaaaacaa
   139381 caataaaaag cctcttttta acctttatct tgtttttgtg tgttcaagta ataactcaat
   139441 tgctttttcc ggtcggtttc gattctttgc accctacagg agataataga ctttactttt
   139501 taggaagaaa gaacatagct accccgtata tcattgttgg attaggtggt ttttacttat
   139561 taaataaaaa aatgaatgag tttatatcac taaaggagat tattttttta ggattgttcg
   139621 gaattttatc atttttaaca caatccagta cagcgattat atgttatggg ctttttattt
   139681 tcatacgtct attgggatta aaggaaaata taggaaaact atattcttta gtcagtatgg
   139741 ctgtatatgt ttgtttttct ctttctataa tcttttcaca aagtacaatt ttatcaactt
   139801 ttacagcgat tttttccaaa aatgcaactt tttcaggacg tatcaatatt tggcaattag
   139861 cgattcgtat ttttgaagag aatttttggt ttggacgagg cctcaacgtt aattttaatg
   139921 cttggacaaa tgggattatc gttaattcag cacacaatac cttattagat atacttgctc
   139981 gaactggtat atttccaggt gttttattta tagtgcttct tatgaatctg tttcttggaa
   140041 aatatagaat agaatcaaaa acactgttgg ctatgttagc ctcatttatg atttatatta
   140101 caatggagac ttcatctatg agcatattgc tgctaattat tgttctttgt gtatattggc
   140161 cacgtggaga ggagaagttg catgagcaag ttacttaata ccatctatta tcgactattt
   140221 agattatata aaaaaattta tctacaagtt tttagtaaaa aaacggttga catcttgggt
   140281 gaaaaggcta atttagaaaa tgaactaatt gtttcgttta caacaattcc aagtcgtctt
   140341 aattatttac caagtatgat taaaagtatt ttcaatcaga caattatccc taaccgtttt
   140401 attatgtatg tttataaaga tgaatttgaa ggcattaatt tggagagtat tctagaactt
   140461 gaaatcaaaa atggtttgga aattgtctat gtggatgaga atcttcgttc acacaaaaaa
   140521 tatttttatg ctatgaagga taatccgaac agtatagttg ttctagttga tgacgatatc
   140581 atttatcctc gaaatactat aaagaaactg attgcatcct atagaattta tccacagtgt
   140641 gtttcagcaa tgcgatgtca tcgaattaag ttgttttctg atggaagttt atatccatat
   140701 aatcaatggg aatatgaaat atcaggagct actataccat cgtattttaa tttttttact
   140761 agcggaggtg ggacattatt tcctccatgt actcgcaatg aggatctttt taataaaaaa
   140821 aatatcaggg aactcagttt tctagctgat gatgtatggt tgaatttttt ggtagtcaaa
   140881 aatggaatta aaacagtaaa agcaacacga tataaaggga caccattaac tatagatgac
   140941 aatcctgagg agagtttggt atatttgaat accgtatatg ataacaacaa tgataaatgt
   141001 attagaaata tggttgaata ttatcaaatt aattttacag aggataaata atgaatgttg
   141061 aaaaaaattt gaaacgtgga attttctata ctgcaatagg gaagtattcg aatgtagtta
   141121 ttcaattact tgtaacagca attcttagcc ggattctctc acctgtagaa tatggtatcg
   141181 tagcagtagt aaatgtattt cttttatttt ttcaaatgtt agcagattca ggaataggac
   141241 ctgctattgt tcaaaataaa gaactgaata agtcagattt aatcagcatt ttctcattaa
   141301 cgatttatag tggaatcgta ctttcttgta tttttgcttt actaggatat cctatggggg
   141361 tgttatacgg cgaaactatt tatgttagtt tatttccgct tttaggtctt tgtgttttat
   141421 tttataccat tacaattgta ccgcaagcta ttctaatgaa aacgatgaac tttaaaatag
   141481 ttaattttct aactattttt tctaacattg ctagtggctt ggtgggcgtt attctagcgg
   141541 tatcacattt tggtgtctat tctctaattt tttctaatat tgtaaaagca atggttttat
   141601 ttatcgcttt atattctaaa gcaaagataa atttctattg gaaagtttca aaaagttcta
   141661 ttggtaaaat ttttgagttt tcaaaattcc aatttttgtt taatttttgg aattattttg
   141721 ctagaaattt agataattta ttaattggac gattgattag cccgtcagat cttggttatt
   141781 atgataaggc ttatcaatta tctttgtacc ctaatcaaat tttatcacag gttgtgacac
   141841 cagcattgca tcctattatg tccaattttc aagataatat cagtaagata ggcgaagttt
   141901 atcttcagat ttctagaata ttcgtaatag ttggcatacc tatttcggct tatttatact
   141961 ttaatgctca atatgttgtt acatttatgt ttggagataa ttggtcccaa agcgttccgg
   142021 tgtttcaaat attagcagca acaatttggt tacaaatggc taatagccct gctggtgcat
   142081 tttaccaagc aactaatcag acaaagttat tgtttagaat tggcttattg acatctttta
   142141 ttaatatttt agctattgtt attggtgtaa tgttgcaaag catccaatgt gttgcgtaca
   142201 tgcttttgat ttctttcagt cttagtttag taatcaatac tctttactta acaaaaaaag
   142261 tattaaatat ttctgctcaa aaatatataa aaccgatatt aattaatttg acaattatca
   142321 gtccctatat tgtgtttaat ttgtttatct cggattttgt aaacgacctt attttacatt
   142381 tatcagttca gtttgttatt ttatttttaa tatggggaat tgggatgtat attagcggag
   142441 agtatcgacg agtcttcgcg gttataagaa aataaaggag caattttgtg aaaattttat
   142501 ttgtgaatat gttttataat gaagcaggag gtgcacaaaa gagcaccaaa tttctagctg
   142561 aacaacttgt aaaagaagga catgaggtat ttgcatactc tgatgatgcc attgattcag
   142621 atattgatga agttattaat agggttaaga tttttagaag aagaaccccg ctttttagtc
   142681 ttcactatat tttcagtgct aagaagaatc cagctaaaca ttttttctat aaaatatttg
   142741 aaacctataa tgtatttgcg aaaagaaaat ttatcaatat cattgaggaa gtacaaccag
   142801 atattgttca ctttaataca atttcaggaa tgtctttatc aatagttaaa gaagctaaaa
   142861 agagaaatat caggaccgtt tggaccttgc gagactattg gctagaatat ccatggggtg
   142921 agaaagatat ttctcttatc aaacttctaa atagaatata tagacctttt gttaagaaaa
   142981 gtttaagaca cttagacata gtaacagcac catcagcatt tactttgaat aattttatca
   143041 ataaaggatt tattaaccca tcttctcaaa aatgtatggt cattgaaaat gctgttatat
   143101 tttcaaaggg taagttggaa aatattatca aacaaaagca agaaacaaaa agaaatccag
   143161 aaaaaacgaa ttttcttttt gtaggttcta tacttgaaat aaagggcgtt tttaacttag
   143221 taaaggcctt taaaaaacta actagtccgg aaatttcttt gaaaattgta ggaaaaggaa
   143281 aagatttaga aaaacttcaa aaagaaattg agagtgatcc tagaatacaa tatttaggct
   143341 ttcaagatac agaaaccctc tataaaactt atcaaaattc ggatgtattg gtcgtcccat
   143401 ctgcttggga tgaaccgttt ggtagagttg taattgaagg aaatgcaaat ggccttcctg
   143461 tcatcacttc agatagaggt ggattacctg aaattgtgca aacagtagga ggaggagaaa
   143521 tttttacaga tgaatcggga aatacattat atagtctttt aaagaaattt gtagaaatgg
   143581 aaaactacaa tgtctattac caatgcattc ttgagaatat tgatatttat tctatcgaaa
   143641 aacaaagtga aaagttcact aatctgtatt tagataaaga aatcgaataa taagttgttt
   143701 ttgtagcagt aacggaggta attatgaaaa tagcggtagc aggaacaggt tatgtaggat
   143761 tatcaattgc tgtgttgttg gcacaacatc acgaaataaa agcagttgat attgttgctg
   143821 aaaaagttga attaatcaat caaaaaaagt ctccaattca ggatgattat attgaaaaat
   143881 atttagtgga aaaagattta aatttggaag caactatgga tgctaagtat gcttatgaag
   143941 atgcagaatt tgttgttgtt gcagcaccaa caaattatga ttcgaaaaag aacttttttg
   144001 atacatcagc agtagaggcg gtcattcaac aagtaaaaaa aattaatccc aaagcggtaa
   144061 taattattaa atctacaatc cctgttggat tcacagagtc cgttcaaaag aaatatcagt
   144121 ataaaaatat cttatttagt ccagaatttt tacgcgaatc taaagcatta tacgataacc
   144181 tgtatccaag tcgcatcatt gttggaactg acttagaaga tacttattta aaagacaaag
   144241 ctaatatatt tgcttcccta ctaaaagagg gggctattaa agaagacata gatacacttt
   144301 tcatgggagt aacggaagct gaggcagtta aattatttgc caatacctac cttgcgttgc
   144361 gtgtttctta ctttaatgaa ttggatactt atgcagaggt gaaaggtttg gatgcaaaag
   144421 ccattattga tggtattggc ctagatccac gtataggaaa tcattataac aatccatcct
   144481 ttggctacgg tgggtattgt ttacctaaag atagtaagca gttattagca aattactttg
   144541 atgtaccaca aaatatgatg acagcaactg ttgaaagcaa tcgaacgcgt aaggatttta
   144601 tcgctcaacg gattttagaa aaagctggag tatctgagac tgatagttta gatgcgttta
   144661 agaacataca agatattgtt ataggtattt atcgtttaac aatgaaaagt aattcggata
   144721 acttccgtca ctcctctatt caaggcgtta tgaaacgatt gaaagctaag ggagttacgg
   144781 ttatcatcta tgagccaact ttgaaggacg gggaaacatt ctttggtaat aaagtggtaa
   144841 ataatctaga taaatttaaa gaagctagta atgttattgt agccaatcgt tttgaacctt
   144901 cactagagga tgtgtctaat aaagtttata gtcgagatat atttaagcgt gactaataaa
   144961 tacgtccacg tgacgtaata tctattaaat gaataaaata ttaataaaca tggcatcgat
   145021 atcttttttg gacttgaatt gtcaaattaa gtgcacattt ttttcataga aaacttcata
   145081 aggtgttttc cggttaagac attttcgtgg tctattatct aagttggttt atataataga
   145141 cttcctgcga aacaaaatat ggtataatag ttctatgaat tatgaagcaa gtaaacaact
   145201 aaccgatgta cgatttaagc gtct
//
LOCUS       DBEJAQ010000002        80834 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.002, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000002 DBEJAQ010000000
VERSION     DBEJAQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 80834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.002"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..210
                     /locus_tag="ACL297_000149"
     CDS             <1..210
                     /locus_tag="ACL297_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGR0070479.1"
                     /translation="KGNSQDNGMMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYID
                     YYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(338..586)
                     /locus_tag="ACL297_000150"
     CDS             complement(338..586)
                     /locus_tag="ACL297_000150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070480.1"
                     /translation="MASTSLQAPAKICFILIKLYSPYAFLSKYLGAILLSFFCYYAKE
                     IGCNNLTLSVWNDNEGALRFYQRQGMKPQETTMEMIID"
     gene            complement(534..1616)
                     /locus_tag="ACL297_000151"
     CDS             complement(534..1616)
                     /locus_tag="ACL297_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGR0070481.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1742..2524)
                     /gene="gatD"
                     /locus_tag="ACL297_000152"
     CDS             complement(1742..2524)
                     /gene="gatD"
                     /locus_tag="ACL297_000152"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HGR0070482.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2524..3867)
                     /gene="murT"
                     /locus_tag="ACL297_000153"
     CDS             complement(2524..3867)
                     /gene="murT"
                     /locus_tag="ACL297_000153"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HGR0070483.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3966..5282)
                     /locus_tag="ACL297_000154"
     CDS             complement(3966..5282)
                     /locus_tag="ACL297_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HGR0070484.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5475..6701
                     /locus_tag="ACL297_000155"
     CDS             5475..6701
                     /locus_tag="ACL297_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HGR0070485.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6900..8474
                     /locus_tag="ACL297_000156"
     CDS             6900..8474
                     /locus_tag="ACL297_000156"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGR0070486.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8739..9527
                     /gene="codY"
                     /locus_tag="ACL297_000157"
     CDS             8739..9527
                     /gene="codY"
                     /locus_tag="ACL297_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGR0070487.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9527..10102
                     /locus_tag="ACL297_000158"
     CDS             9527..10102
                     /locus_tag="ACL297_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGR0070488.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10184..10269
                     /locus_tag="ACL297_000159"
     tRNA            10184..10269
                     /locus_tag="ACL297_000159"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10218..10220,aa:Leu,seq:aag)
     gene            10552..11682
                     /locus_tag="ACL297_000160"
     CDS             10552..11682
                     /locus_tag="ACL297_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070489.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            12183..12941
                     /locus_tag="ACL297_000161"
     CDS             12183..12941
                     /locus_tag="ACL297_000161"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGR0070490.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13029..13541
                     /locus_tag="ACL297_000162"
     CDS             13029..13541
                     /locus_tag="ACL297_000162"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGR0070491.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13723..14667
                     /gene="metA"
                     /locus_tag="ACL297_000163"
     CDS             13723..14667
                     /gene="metA"
                     /locus_tag="ACL297_000163"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGR0070492.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14676..15353
                     /locus_tag="ACL297_000164"
     CDS             14676..15353
                     /locus_tag="ACL297_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGR0070493.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15451..16209
                     /gene="tpiA"
                     /locus_tag="ACL297_000165"
     CDS             15451..16209
                     /gene="tpiA"
                     /locus_tag="ACL297_000165"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGR0070494.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16247..17752
                     /gene="lytC"
                     /locus_tag="ACL297_000166"
     CDS             16247..17752
                     /gene="lytC"
                     /locus_tag="ACL297_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HGR0070495.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18250..18768
                     /locus_tag="ACL297_000167"
     CDS             18250..18768
                     /locus_tag="ACL297_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HGR0070496.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            18898..19404
                     /locus_tag="ACL297_000168"
     CDS             18898..19404
                     /locus_tag="ACL297_000168"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HGR0070497.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEKKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19404..19574
                     /locus_tag="ACL297_000169"
     CDS             19404..19574
                     /locus_tag="ACL297_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070498.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            19606..20838
                     /gene="clpX"
                     /locus_tag="ACL297_000170"
     CDS             19606..20838
                     /gene="clpX"
                     /locus_tag="ACL297_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGR0070499.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20847..21434
                     /gene="yihA"
                     /locus_tag="ACL297_000171"
     CDS             20847..21434
                     /gene="yihA"
                     /locus_tag="ACL297_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGR0070500.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHAPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21445..21825
                     /locus_tag="ACL297_000172"
     CDS             21445..21825
                     /locus_tag="ACL297_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGR0070501.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21877..22767
                     /gene="rapZ"
                     /locus_tag="ACL297_000173"
     CDS             21877..22767
                     /gene="rapZ"
                     /locus_tag="ACL297_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGR0070502.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22764..23741
                     /locus_tag="ACL297_000174"
     CDS             22764..23741
                     /locus_tag="ACL297_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HGR0070503.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23738..24649
                     /gene="whiA"
                     /locus_tag="ACL297_000175"
     CDS             23738..24649
                     /gene="whiA"
                     /locus_tag="ACL297_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGR0070504.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24683..25651)
                     /locus_tag="ACL297_000176"
     CDS             complement(24683..25651)
                     /locus_tag="ACL297_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGR0070505.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEDYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQTLQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25787..26644
                     /gene="cdaA"
                     /locus_tag="ACL297_000177"
     CDS             25787..26644
                     /gene="cdaA"
                     /locus_tag="ACL297_000177"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGR0070506.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26631..27410
                     /locus_tag="ACL297_000178"
     CDS             26631..27410
                     /locus_tag="ACL297_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HGR0070507.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27434..28786
                     /gene="glmM"
                     /locus_tag="ACL297_000179"
     CDS             27434..28786
                     /gene="glmM"
                     /locus_tag="ACL297_000179"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGR0070508.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28860..29234
                     /locus_tag="ACL297_000180"
     CDS             28860..29234
                     /locus_tag="ACL297_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HGR0070509.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPNELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29236..30084
                     /locus_tag="ACL297_000181"
     CDS             29236..30084
                     /locus_tag="ACL297_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGR0070510.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30361..31128
                     /gene="dapB"
                     /locus_tag="ACL297_000182"
     CDS             30361..31128
                     /gene="dapB"
                     /locus_tag="ACL297_000182"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGR0070511.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31125..32324
                     /locus_tag="ACL297_000183"
     CDS             31125..32324
                     /locus_tag="ACL297_000183"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGR0070512.1"
                     /translation="MRLMQMPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIH
                     DVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSA
                     VSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDA
                     LRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLA
                     SMIESQAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKS
                     WKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDLDLLLQAENLRQAQGKEVNPQVI
                     KETYQSLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIH
                     AYLREKK"
     gene            32321..34192
                     /locus_tag="ACL297_000184"
     CDS             32321..34192
                     /locus_tag="ACL297_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGR0070513.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAQDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34335..35519)
                     /gene="mntE"
                     /locus_tag="ACL297_000185"
     CDS             complement(34335..35519)
                     /gene="mntE"
                     /locus_tag="ACL297_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HGR0070514.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35823..38519
                     /locus_tag="ACL297_000186"
     CDS             35823..38519
                     /locus_tag="ACL297_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGR0070515.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38532..39323
                     /gene="yghU"
                     /locus_tag="ACL297_000187"
     CDS             38532..39323
                     /gene="yghU"
                     /locus_tag="ACL297_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGR0070516.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLRTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39439..39849
                     /locus_tag="ACL297_000188"
     CDS             39439..39849
                     /locus_tag="ACL297_000188"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGR0070517.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39861..41456
                     /locus_tag="ACL297_000189"
     CDS             39861..41456
                     /locus_tag="ACL297_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070518.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGRTFEGQVGKDSYLKTFKVQ"
     gene            41635..41799
                     /locus_tag="ACL297_000190"
     CDS             41635..41799
                     /locus_tag="ACL297_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HGR0070519.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKENKDKFLSILTSLAGLALV
                     LVAVWLGWPK"
     gene            41878..42606
                     /gene="yaaA"
                     /locus_tag="ACL297_000191"
     CDS             41878..42606
                     /gene="yaaA"
                     /locus_tag="ACL297_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGR0070520.1"
                     /translation="MKILIPTAKEMNTDLPSIETTLLRSESQAVLDALALYSASQLES
                     FYKVSAEKAAEECQNIQALKRQTAQHYPALKLFDGLMYRHIKRDKLTEVEQAYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDEAVKQEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(42657..44141)
                     /locus_tag="ACL297_000192"
     CDS             complement(42657..44141)
                     /locus_tag="ACL297_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator PRD
                     domain-containing protein"
                     /protein_id="HGR0070521.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            44357..49681
                     /locus_tag="ACL297_000193"
     CDS             44357..49681
                     /locus_tag="ACL297_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HGR0070522.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETK
                     PAVTTTERSAVDSAILPISTAAKVETTSAPASTNEILKPSLSLSDARQNPAIRKEDVD
                     RGYSGFRTAGSGFRAAGSGFRAAGPENKPILNPNNTIAFSDISQGLHSFRGIGHSRGG
                     REIHYDVTTVRRGNRLNFTIKYSGPGEFVNNNFILDKGDGFGNPSNATITSSNPRVRE
                     QSKSISQGANYVSHSGYSMTSATSTNTEQTIRFSLPIINPNGDLSVRLKPVTFNVDQG
                     GGGAATSNDPYSNSNYYHRANPLLLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQ
                     TRLVREGEKGQRQITYKVHRFGNETLLGLPISNRVTKEAKPRIMQIGVAKELIDTVKP
                     RVDQNKVGDTNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAQ
                     GTKGEKGDQGERGLTGAQGAKGEKGEQGLQGRDGEQGPKGEDGKTPTVKVTDGQDGTH
                     TITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTK
                     LKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKN
                     GEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGAS
                     GRDGKEVLNGKADPTTEGKDGDTFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGA
                     KGEKGDRGERGLTGAQGTKGEKGDQGERGLTGAQGTKGEKGDRGERGLTGAQGQAGRD
                     AVTPTVTVKDNKNDGTHTITINDGRGNVASTVVRDGFDGASPLVATQRNEADKTTTVI
                     FYYDQNGNNELDASDKKLKEVIIADGAKGEQGLQGRDGQDGAQGQAGRDGKDVLNGKA
                     NPEVNQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTP
                     GKDGHSTDITFTVPGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRDTDAAATP
                     TRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTKDNGDGTHTI
                     TVQNPDSSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVVKNGKDGK
                     TPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSHTVTITNP
                     DGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRDGKSPKVT
                     ITDEQNGTHKISVLNGDGTTTETIIKDGKSPVATVRDNQDGTYTIRVENGNGTVSETT
                     VRDGKSPTAKVVDNGDGTHTITVVNSDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVT
                     GADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPT
                     VTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPT
                     PEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPV
                     NANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKED"
     gene            complement(49918..50466)
                     /locus_tag="ACL297_000194"
     CDS             complement(49918..50466)
                     /locus_tag="ACL297_000194"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGR0070523.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            50787..51275
                     /locus_tag="ACL297_000195"
     CDS             50787..51275
                     /locus_tag="ACL297_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HGR0070524.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            51272..52459
                     /locus_tag="ACL297_000196"
     CDS             51272..52459
                     /locus_tag="ACL297_000196"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGR0070525.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            52452..52817
                     /locus_tag="ACL297_000197"
     CDS             52452..52817
                     /locus_tag="ACL297_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGR0070526.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            52833..54179
                     /gene="asnS"
                     /locus_tag="ACL297_000198"
     CDS             52833..54179
                     /gene="asnS"
                     /locus_tag="ACL297_000198"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGR0070527.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            54332..54622
                     /gene="rpsF"
                     /locus_tag="ACL297_000199"
     CDS             54332..54622
                     /gene="rpsF"
                     /locus_tag="ACL297_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGR0070528.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            54634..55104
                     /gene="ssbA"
                     /locus_tag="ACL297_000200"
     CDS             54634..55104
                     /gene="ssbA"
                     /locus_tag="ACL297_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HGR0070529.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            55136..55375
                     /gene="rpsR"
                     /locus_tag="ACL297_000201"
     CDS             55136..55375
                     /gene="rpsR"
                     /locus_tag="ACL297_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGR0070530.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            55670..57070
                     /locus_tag="ACL297_000202"
     CDS             55670..57070
                     /locus_tag="ACL297_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HGR0070531.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            57072..57431
                     /locus_tag="ACL297_000203"
     CDS             57072..57431
                     /locus_tag="ACL297_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGR0070532.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            57485..58234
                     /locus_tag="ACL297_000204"
     CDS             57485..58234
                     /locus_tag="ACL297_000204"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGR0070533.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            58224..58523
                     /locus_tag="ACL297_000205"
     CDS             58224..58523
                     /locus_tag="ACL297_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGR0070534.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            58594..59529
                     /locus_tag="ACL297_000206"
     CDS             58594..59529
                     /locus_tag="ACL297_000206"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HGR0070535.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            59615..60127
                     /locus_tag="ACL297_000207"
     CDS             59615..60127
                     /locus_tag="ACL297_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGR0070536.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            60111..60760
                     /locus_tag="ACL297_000208"
                     /pseudo
     CDS             60111..60760
                     /locus_tag="ACL297_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            60861..61058
                     /locus_tag="ACL297_000209"
     CDS             60861..61058
                     /locus_tag="ACL297_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGR0070537.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(61101..62546)
                     /locus_tag="ACL297_000210"
     CDS             complement(61101..62546)
                     /locus_tag="ACL297_000210"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HGR0070538.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVKDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            62652..64274
                     /locus_tag="ACL297_000211"
     CDS             62652..64274
                     /locus_tag="ACL297_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGR0070539.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            64478..66436
                     /locus_tag="ACL297_000212"
     CDS             64478..66436
                     /locus_tag="ACL297_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0070540.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            66608..66841
                     /locus_tag="ACL297_000213"
     CDS             66608..66841
                     /locus_tag="ACL297_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HGR0070541.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            66859..67380
                     /locus_tag="ACL297_000214"
                     /pseudo
     CDS             66859..67380
                     /locus_tag="ACL297_000214"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
     gene            67567..68289
                     /locus_tag="ACL297_000215"
     CDS             67567..68289
                     /locus_tag="ACL297_000215"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070542.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPSGSGKSTIV
                     RLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGRSQRLQIARAYLKDAEIL
                     IFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLEEGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            68447..69541
                     /locus_tag="ACL297_000216"
     CDS             68447..69541
                     /locus_tag="ACL297_000216"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGR0070543.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            69549..70715
                     /locus_tag="ACL297_000217"
     CDS             69549..70715
                     /locus_tag="ACL297_000217"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGR0070544.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            70808..73906
                     /locus_tag="ACL297_000218"
     CDS             70808..73906
                     /locus_tag="ACL297_000218"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGR0070545.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            73954..74571
                     /locus_tag="ACL297_000219"
     CDS             73954..74571
                     /locus_tag="ACL297_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGR0070546.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            74582..75916
                     /gene="murC"
                     /locus_tag="ACL297_000220"
     CDS             74582..75916
                     /gene="murC"
                     /locus_tag="ACL297_000220"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGR0070547.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            75926..76390
                     /locus_tag="ACL297_000221"
     CDS             75926..76390
                     /locus_tag="ACL297_000221"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070548.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            76387..76881
                     /locus_tag="ACL297_000222"
     CDS             76387..76881
                     /locus_tag="ACL297_000222"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070549.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            76962..78617
                     /gene="mltG"
                     /locus_tag="ACL297_000223"
     CDS             76962..78617
                     /gene="mltG"
                     /locus_tag="ACL297_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGR0070550.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            78688..79170
                     /gene="greA"
                     /locus_tag="ACL297_000224"
     CDS             78688..79170
                     /gene="greA"
                     /locus_tag="ACL297_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGR0070551.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            79588..79986
                     /locus_tag="ACL297_000225"
     CDS             79588..79986
                     /locus_tag="ACL297_000225"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070552.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            80431..>80834
                     /locus_tag="ACL297_000226"
     CDS             80431..>80834
                     /locus_tag="ACL297_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGR0070553.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLS"
BASE COUNT        24488 a        14960 c        18227 g        23159 t
ORIGIN      
        1 aagggaaaca gccaagacaa cggtatgatg gagtccttct ttgggattct gaaatcggaa
       61 atgttttacg gttatgagaa gtcgtttcag tcgcttaagc aattggaaca agctattgta
      121 gactatattg attactacaa caataaacga attaaggtaa aactaaaagg acttagtcct
      181 gtgcaataca gaactaaatc cttcggataa attaattgtc taactgtttg ggggcagtac
      241 attttaacac ttgatactat gcgttttatt gtgggaagat tttcctcatt ttctagtgaa
      301 attgaacttt ttctaacatc ttttgataga tgacttctta atcaattatc atttccattg
      361 ttgtttcttg gggtttcatc ccttgtcttt gataaaagcg gagagcaccc tcgttatcat
      421 tccagacact tagggttaga ttattgcagc caatttcttt agcataatag cagaaaaatg
      481 acaataaaat agcccctaga tattttgaaa ggaaagcata ggggctatat agtttaatca
      541 aaataaagca aatctttgct ggtgcttgta aagaggtcga agccatcctt ggtaacaaca
      601 ccgcagtctt caatacgaac accgacttta ccagggatat agatacctgg ttcaacagag
      661 aagcacatgc cttcttcgat gaccatgtcg tttccttcca tgatagatgg gaattcatgg
      721 acatccatac cgataccatg cccgagacgg tggttgaagt actcaccata accagctttt
      781 tcgatgacct cacgggcagc gcggtccact tcatgagcag tcacacctgg cttgataaag
      841 tcaagagcag cttgttgggc ttcaagagtc aagttgtaaa tatctttctt gaattggtct
      901 ggtttgccga cagcgactgt acgagtcata tctgacgcat agccattgac cagaacaccc
      961 aggtcaaaga ggagaagagc atcattttca accttattag ctgctggaat gccgtgtgga
     1021 ttcgcagcat tatcaccagt caagaccatg gtatcaaagc tcatttcata accttcacgt
     1081 ttcatggcaa agtcgatttg tgcgatgata tctgtctcag tcttatcaag agaaatattg
     1141 tcaaaaccaa catgaacagc cttgtcagca taaagacctg caaccatcat tttttgcact
     1201 tcatcagctg atttgatgag gcgcatgcgt tggatacgag gagtgaggtt gtcaaactca
     1261 gcagtctcaa aaactgtttt caaaccatgg tatttggtca agatgagatt gtcaaactca
     1321 acagcgacac gtttgaagtc aagttgtgga agagcatgtt tgattttttg ccatggattt
     1381 tcagaatcga catagcccac tactgggaag gaaacggtgc tacttgcacg ttctacttca
     1441 agagctggga caaagaggag aggttcctga tccgctagga caaagaggaa catttggcgt
     1501 tcatggggat cactgtaaaa accagtgagg taattgattg tgacggggtc agatacgaca
     1561 gcgacgtcta gtttttctga ttcaagatat gttaggattt gttgtaattt agacatatgc
     1621 taccttcttt ctacccctct attttggcaa aaaatgagag aaaatgcaag ggagaagggt
     1681 aaaagaaaag acaaaaaact ccgacaagat aacggagttc tttgatatca ttttcctttg
     1741 tttaagaaaa gtcagccttg cttttgacgt cactgtactc ttcagcgatt tcttggctga
     1801 gaatgtcctc ataggcaggg agttggatgt cctgaccata tttcttcttg agggcagtag
     1861 taactaggcg ataagccaga ttggcattac gagagaggat aggcccgtgg aagtaggaac
     1921 caaagacatt cttataatga accccttcac cgaccttttc ttcgttgttt ccatttccat
     1981 agacaacctg tcccagcggt ttttggtcat cagagaggaa ggtacgacct tggtgatttt
     2041 caaatccata gtaggtttca tcgaaatctt cattgtgaat cttgatgtca ccgataaaac
     2101 ggttattggt ctggttgagc gtgtagtgtc ccatgacccc tagcccttcg atacgttttc
     2161 ctgaagcttc aacataatat tgacccaata gttggaaacc accgcagata gccagaacta
     2221 caccgtcgtt ttggatgtag ttgtcaatgc tctctttttt agcaggtagg tcgtctgcaa
     2281 tgatactttg ttcaaagtct tgaccaccac cgaaaaaggc gatgtcgtag tgattttcat
     2341 caaagtcatc atggagagaa acgatgtcaa cggtcacatg ggctcccagt ttttcagcca
     2401 catacttgag catgaggatg tttccattgt ccccgtaggt attcatgaga tttccgtaga
     2461 ggtgggcaat gttgagctga taggggtaat tgctatcttt tgaggaaagt gaagtataaa
     2521 ccattagttc atctcctttc taacaatctg acgactagcc agcagttcac gaaattccag
     2581 catggcagta taagttgcca gaatataggc atgcttgcag tcttgattct caatggtctt
     2641 gagaacttgc tccagattac tcgtttcagt gattttctca gctggatagc cagtcactcg
     2701 gaggcgacga gcgatttcag aatgacgaac accgccagcg ttgatttcag gaatgtccat
     2761 gtcagtgatt tgctcaaagt ctgcatccca gatccagcta gtgtcaattc catctgcata
     2821 gttggcatta aggaggacag ataggctaaa tggataaggt gctagtttga tcatttcgat
     2881 agcttgggtt gcaccgactg gatttttaat caagacaagg gtacattcct tgtcaccgat
     2941 atgaaaggtt tcttggcgtc caaagacagc acggctcttg tcaaatccct gtttgatgag
     3001 ttgggaatct gcgccgagga aacgggcgat ggccacagca gctagggcgt tatagatatt
     3061 atagagcccg ccgatttgga taccgtattc ttggccgtct atgacaaagc gagagcgatt
     3121 gttggtcaac tcaaccagtt ttgtcaaacg atagtcgaga tcaggacgtt tacatccaca
     3181 accttcacag atataggcac ccaagtttgc ataggtatta tgctcatatt tgaggatgcc
     3241 ttggcagtca ggacagagaa tcccttcggt attgtagtga gcaagttggg ctggtccttt
     3301 ttccaagtca aaaccaaaat actctatagg gtttggaata gttggcttgt agaaaagtgg
     3361 actgtctccg ttaaggagaa cagtagcagt tggaactttt cgaatggcat ccaatatcat
     3421 gttataggta gtatagattt caccgaaacg gtccatctgg tcacggaaga tattagtaat
     3481 gacaaaaaga ctaggctgga tatagtcaca gatacgagat agactggctt cgtcaatttc
     3541 gaggacggca atatttttcc cagtttttga agatttggct gttaggaagg ttgttgcaat
     3601 ccctgtaatc atgttggcac cgcttgggtt ggttagaact tgaccataaa cctcttttaa
     3661 aatgccgaca gtgagggcag ttgtcagggt ttttccattt gttccagtga caacgacaat
     3721 ctcgtagttc ttagctaggc tttgtaaaat atctttatca aattgaaggg cgactttccc
     3781 tgggagcgta cttccacgtc caagacggct taaaacgaag tgggaagaac gcccagcaag
     3841 aaggcccaaa gtagttttta agttcatgtt tctattatat cacaaaagag ggaaaagaca
     3901 gatttggtgt ttcgtagaag taaaaacagc ccaaagaggg ctgtcagtta agatgagatt
     3961 tcaacttaaa tcgcaaacaa gtcattcaag ttctcagcca aggttgggtg agtgaagatt
     4021 tgttttgtga agtaagtgta aggaatcttg ttgtccatag caacagtaat gatgttgatg
     4081 atttcttgag aaccttctga gaagatgctt gcaccaagaa tttcttttgt ttcagtatta
     4141 acaacagctt tgaaagctcc gcgaaggtct ccatttacgt gaccacgagg catggctgca
     4201 acagggattt ctttcactgc gtatggaagt ttcaaatcag ctgcttggct ttcagtcaaa
     4261 ccaacttgtg aaagtgcagg tgtgatgaac atagtatttg gcacattgag acggtcctca
     4321 agtgtgtagc tgccatctcc agcaaggtag ctgtaaacaa cacggaagtc atcaagtgaa
     4381 atgtaagtaa attgaaggcc accgttgaca tctccaactg caaagacacc aggaacgttt
     4441 gtttgacagt gtttgtctac tttaatagca ccacgttcag ttagttcaat atctgtattt
     4501 tcaagttgaa gtggttctac atttggtttg cgtccagttg cgtagagaag ggcgtcgaaa
     4561 cggtaagttt cgtcttcagt tacgacaagc acttggtcac catcgttttt gatttcagta
     4621 gtatggatat tttgaagcaa ttcaatgcca tcttcttcca tgtattgttt agcaagagct
     4681 gcgatggaag gttctgcacg aggtaggaat gtatccaagg catctaggac tgtgaccttg
     4741 cttccaagtt tgttgtaaag gccggcaaat tcaagaccga tatttccgcc accaaggatt
     4801 ccaagttttt caggtaattt gtccaagctt tggatacctg ttgagtcaaa gatgtttttg
     4861 cttgtagcaa gtccaggaat tggcaagacg tttgaaacag caccagtgtt gatgacgatt
     4921 gtttcagcag tcagttcttt cttttcatca ccagcttgga tttcgatgac tttatttgaa
     4981 aggaagtgag cttccgcatc aaagatatct acgcctgtac cagcaacagt cgcatagttt
     5041 ttaccgttga ggcgaccagt gatcgtgttt ttagtagcaa tcacttcttc aaaagacaag
     5101 tccttttcag cagcaactag caaggtttta gttgggatac aaccaatgtt gatacaagtt
     5161 ccaccgtaca tagccttgct gcgttcaacg agggcaactt ttttgccagc tgaagccaat
     5221 ttacctgcta gtgttttacc agctttacca aatccaataa caattaaatc gtatgttaac
     5281 atttatcttc ctcctattcg aatttcattc tagcacaaga aaaaaacttt tgcacaattc
     5341 tgctacggaa aagacagaaa aagataaaaa cgctttccag acaaaaaact agtaattttt
     5401 tgacaagctt gataaactaa aatcctatct atttttatat aagaaagaat aaatgttcga
     5461 aaataggagg ttttatgaaa tcgaatcgtt atattattgc ctttgctggg gtcattttac
     5521 acttaatgct gggttcgact tatgcttgga gtgtttatcg taaccctatt attgaaaaaa
     5581 cgggatggga tcaggcttct gttgccttcg cctttagtct agcaatcttt tgtttgggct
     5641 tatcggctgc atttatgggg cgtttggtag aaaaatttgg tccgaaagtc atggggagtc
     5701 tatctgcttt tctatacgca ggtggaaata tcttaacagg atttgcaata gaccgtcagg
     5761 aactgtggtt gttgtatcta gcttatggca ttttaggtgg gcttggtttg ggagcaggct
     5821 atattacccc tgtgtcaacg attataaaat ggtttcctga taaacgtggt ctcgcaacag
     5881 gtttagcgat tatggggttt ggttttgctt ctttattgac tagtcccata gcgcaacacc
     5941 tcatcgcagg ggtagggatt gtagaaactt tttatatttt aggagcaagt tactttatta
     6001 tcatgctcct agcttcacaa ttcattaagc gtccaaatga gcaagagctt gcaattttat
     6061 cttcttcagg gaaagaaaaa acagcctctt tgacgcaagg aatggctgca aatcaggctc
     6121 taaaaagcaa tcggttttat atgctttgga ttattttctt tatcaacata gcttgtggtt
     6181 taggcttaat ttcagcggca tcgccaatgg cacaggagat ggctggcttg tctacaagtc
     6241 atgcagcagt aatggtgggt gttttgggga ttttcaatgg atttggtcgc ttgctctggg
     6301 cgagtttgtc tgactatatc ggtcgccctc taacctttag tatattactg cttgtcaatc
     6361 ttttcttttc tctctcactt tggctcttta cagattccgt tttatttgta gttgctatgt
     6421 ctattttgat gacttgctat ggagctggtt tttctttgat tccagcttat ctcagtgata
     6481 tttttggaac caaggaattg gccgctctgc atggttatat tttaacagct tgggcaatgg
     6541 ctggtttagc gggacctatt ttattagcag agacttataa aatggctcat tcgtacacac
     6601 aaaccttgtt cgtttttctc attttataca gtatcgcctt ggctttgtct tattatctag
     6661 gtcgttcaat caaaaaagaa agtcaaaaac cgcttacatg attttctgta gaaatggggc
     6721 tatcttttgc tctggtcttg tgttatacta gataggttgc aaagaaaaca gtacttttct
     6781 tttgtggaaa agaagcaaca cgattttata tctagttata tgaaaatacg tcataaaaag
     6841 aaaagtataa actaagccct atagggtggc ttcgcaccac ctttagaaag aagaataacg
     6901 tgaaatttaa tgaattaaac ttgtctgctg atttgctagc agaaattgaa aaagctggtt
     6961 ttgtagaagc tagtcctatc caagaacaaa ctattccttt ggcccttgaa ggcaaggatg
     7021 ttatcggtca agctcagact ggtacaggaa aaactgcagc ctttggcttg cctacccttg
     7081 aaaaaatccg tatagaagaa gcgactattc aagccttggt catcgctcca actcgtgaac
     7141 tagctgtcca aagtcaagaa gaactcttcc gctttggtcg tagtaaggga gtcaaagtcc
     7201 gttcagtata tggcggatca agcattgaaa aacaaattaa ggctcttaaa tctggtgccc
     7261 atatcgtggt gggaactcca ggtcgcctct tggacttgat taaacgcaag gccttgaaat
     7321 tacaagacat tgaaaccctt atccttgacg aagcggatga aatgcttaac atgggcttcc
     7381 ttgaagacat cgaagccatt atttcccgtg tacctgagaa ccgtcaaact ttgcttttct
     7441 cagcaactat gccagatgcc atcaaacgta tcggtgttca gtttatgaaa gcccctgaac
     7501 atgtcaagat tgcggctaag gaattgacaa cagaattggt tgaccagtac tatatccgtg
     7561 ttaaggaaca agaaaaattt gacaccatga ctcgtctcat ggatgtggca caaccagaac
     7621 tcgctattgt atttggtcgt accaaacgcc gtgtggatga attgactcgt ggtttgaaaa
     7681 ttcgtggctt ccgtgcagaa ggaattcatg gcgacctaga ccaaaacaaa cgtcttcgtg
     7741 tccttcgtga ctttaaaaat ggcaatcttg atgttttggt tgcgacagac gttgcagcgc
     7801 gtggtttgga tatttcaggt gtgacccatg tctacaacta cgatattcca caagatcctg
     7861 agagttatgt tcaccgtatc ggtcgtacag gtcgtgctgg taagtcaggt caatctatta
     7921 cttttgttgc tccaaacgaa atgggttacc ttcaaatcat tgaaaacttg actaagaaac
     7981 gcatgaaagg tctcaaacct gcaagtgtag aagaatcctt ccaatcaaaa aaacaggtag
     8041 ctctcaagaa aatcgaacgt gattttgcag atgaaaccat tcgtgccaac tttgagaaat
     8101 ttggtaagga tgctcgcaaa ttggctgctg agtttactcc agaagaattg gcaatgtata
     8161 tcttgagtct gacagtccaa gacccagata gccttccaga agtggagatt gcacgtgaaa
     8221 aaccactacc gtttaaacca tcaggtaatg gtttcggtgg taaagctaag ggaggtcgtg
     8281 gaggccgtcg tggggacgac cgtcgagagc gtgatcgtcg tggcaatggt cgccgtgatg
     8341 agttcaaaaa aggaagtcgt ggcaacgatc gttttgataa ggaaaaacgt taccgtaagg
     8401 ataataaaaa accacgcaat actttaagcg aaaagcaaac aggctttgtt attcgtaaca
     8461 aaggtgataa ataagaaaaa gcggttcaaa atgaatcgct tttttatata aggagaccga
     8521 atgtgaaaaa gacaaataaa attattatat tatcttttat aatattatga tacacaaaaa
     8581 taggcgactt gtcaatagaa aaggaacaat ttaattaaaa atattctggt ttttaattaa
     8641 attgtaagtc aatgcaagtc aataaggaat tttcagataa ttattgaaaa agagacgttt
     8701 ttatgttata atgatagcga ttaaattcga ggtgaaaaat ggcacattta ttagaaaaaa
     8761 ctagaaaaat tacatcaatt ttgaaacgct cagaggagca gttgcaggat gagctccctt
     8821 acaacgctat tacgcgtcag ttagcggata ttattcattg caatgcctgc attatcaata
     8881 gtaagggacg tctgcttggc tattttatgc gttataaaac aaatacagat cgcgtagagc
     8941 aattcttcca aactaagatt ttcccagatg actacgttca aggggctaat atgatttacg
     9001 aaacagaagc aaacttgcct gttgagcatg atatgagtat tttccctgtt gagagtagag
     9061 atgattttcc agatggcttg acgactattg caccgattca tgtatcgggg attcgccttg
     9121 gttctttgat tatttggcgt aatgataaaa aattcgaaga tgaggacttg gttcttgttg
     9181 agattgccag taccgttgtt gggattcagc ttcttaactt ccaacgtgaa gaagatgaga
     9241 aaaatattcg tcgtcgtact gctgtcacca tggcggtcaa taccctttct tactccgaac
     9301 tccgtgctgt ttcagcaatt ttaggggaat taaatggaaa tgaagggaag ttgactgcgt
     9361 cagtgattgc agatcgtatc ggaatcactc gctctgtgat tgtcaatgct cttcgtaaac
     9421 ttgagtctgc ggggattatt gaaagtcgct cacttggaat gaagggaacc tatcttaagg
     9481 tcttgatttc agatattttt gaagaagtga agaaaagaga ttactaatga caaaggcttt
     9541 aatttcgatt gattatacag aagattttgt tgctgatagt gggaagctga cagcaggtgc
     9601 tccagctcag gcgatttcgg atgccatcag caaggtaact cgattagctt ttgaacgagg
     9661 agattatatc ttctttacta ttgatgctca tgaagaaaac gattgtttcc atccggaaag
     9721 taagttattt cctcctcata atctgattgg gacgagtgga cggaatttat atggagattt
     9781 ggggatcttt tatcaagagc atggttcaga cagtcgtgtc ttttggatgg ataaacgcca
     9841 ttactcagct ttttcaggga ctgacctaga tattcgtttg agagagcgta gagtgtctac
     9901 cgttatctta acaggtgtct tgacggatat ctgtgtccta catacagcta tagatgccta
     9961 taatctagga tatgacatcg agattgttaa accagctgtt gcttccatct ggcctgaaaa
    10021 tcatcaattt gccctaggtc atttcaaaaa tacactcgga gctaagttag tagatgaaaa
    10081 tctaaatgaa ctttctgagt aacctggttg gtgaaaatga aaaaatttga aaaaaagtgt
    10141 tgacaagcat cccaaaagtt gatatactag taaagtaatc gatgcgggga tggcggaatt
    10201 ggcagacgcg caggactaag gatcctgtga ccgctttagg tcgtgagggt tcaagtccct
    10261 ctctccgcat aggaaaagag ttagctaaag ctagctcttt tgtatttctc taaaaaatta
    10321 gaatccagtc aaatgtagtg gagaatcagc gaaaatatgg gaaaactgat ccttgtttct
    10381 gcttttgtca tagaagaata ataaagatta tcttcttcaa attctccgat attctctaaa
    10441 gttttgtgca agttgcacag aactttttta tttttttggt catcttgcca tagaaatata
    10501 aagcgttttc atctataata taattatcaa aagacaaaag gagttcacct catggtagaa
    10561 ttgaatctta aaaatattta caaaaaatat ccaaacagcg aacactattc agttgaagat
    10621 ttcaacttga acatcaaaga taaagaattt atcgttttcg taggaccttc aggatgtggt
    10681 aaatcaacta cactccgtat gattgctggt cttgaagaca tcacagaagg tactgcatct
    10741 atcgatggcg tggttgtcaa cgacgtagct ccaaaagacc gtgatatcgc catggtattc
    10801 caaaactacg ctctttaccc acacatgact gtttatgaca acatggcttt cggtttgaaa
    10861 ttgcgtaaat acagcaaaga agacattaac aaacgtgttc aagaagcagc tgaaatactt
    10921 ggattgaaag aattcttgga acgtaaaccg gctgaccttt caggtggtca acgtcaacgt
    10981 gttgccatgg ggcgtgcgat tgtccgtgat gcgaaagtat tcttgatgga cgaacctttg
    11041 tcaaacttgg atgccaaact tcgtgtatca atgcgtgctg aaatcgctaa aattcaccgt
    11101 cgtatcggag ctacaactat ctatgtaact cacgaccaaa cagaagcgat gacacttgca
    11161 gaccgtatcg ttattatgtc agctactaag aaccctgctg gtacaggtac tatcggacgt
    11221 gtagaacaaa tcggtactcc tcaagaagtt tacaaaaatc cagttaacaa attcgttgca
    11281 ggattcatcg gaagcccagc tatgaacttc atcaacgtga aattggttgg tagcgaaatt
    11341 gtttctgacg gtttccgttt gaaagtgcca gaaggagcat tgaaagttct tcgtgaaaaa
    11401 ggctacgaag gaaaagaatt gatctttggt atccgtccag aagacgtgaa tgcagaacct
    11461 gctttccttg aaacattccc agactgtgtt gtaaaagcga ctatctctgt atcagaactg
    11521 cttggttcag aatctcacct ttactgccaa gttggtaaag acgagtttgt tgcaaaagtt
    11581 gatgctcgtg actacttgca aacaggtgca acagttgagc ttggatttga cttgaacaaa
    11641 gcacacttct tcgatgtaga aactgaaaaa acaatctact aagaataaat aaaattcaaa
    11701 gcactacaag aaaagatatc tctttatcaa ttgtagtgga gagatatcag ttaatctagg
    11761 gagagaaaca aaatgcttct ctcctttttg ctagggaagt catattatac atctatattg
    11821 tgatgctctt tatactcttc gaaaatctct tcaaaccacg tcaacgtcgc cttgccgtac
    11881 gtatgattac tgacttcgtc agttttatct gcaacctcaa agctgtactt tgagtagctt
    11941 acggctagtt tcctagtttg ctctttgatt tccattgagt attatttgtg ggtaccatct
    12001 acaagtgaag ctatatgcgt aaactacgtg agcaattgaa ttcgaactag agaggtaata
    12061 ataaatttat gctatagtta tggtgacttg tatgcttttg attctagttt atcaaataat
    12121 agattagaat tgtcagataa tatcattttg tgttataatg aagaaaaaac agaggtgttc
    12181 aaatgtcaga agcaggtcat aagtttttag caaaattggg gaaaaaacgc ttacgtccag
    12241 gtggaaagcg tgccacagat tggttaattg cagaaggagg attttcaaaa gaaaagagaa
    12301 tactagaggt tgcgtgtaat aggggaacta cagcaattga gttggcacag cgttttggtt
    12361 gcaagataac tgctgttgat atggatgctc aagctttaga agtggctaaa aaatctgctg
    12421 gaacggcagg tgttgctcat ttaatcagtt ttgaaagagc aaatgcaatg aaacttcctt
    12481 atcaagatgc tagttttgat attgttataa atgaagctat gctgactatg caagccgatc
    12541 aagctaagaa aaaatgtgta atggaatatc taagggtatt aaaacctgga ggtcttctct
    12601 tgacacatga tgtgcttctt aaggaagcta aagagtctat cagacaggaa ttatcacaag
    12661 caattcatgt aaatgtaggt cctttaactc aagatggttg ggaacaggtg atgatagaat
    12721 caggttattg tgatgtgaaa gcattgactg gtgaaatgac attaatgaaa ttatcgggta
    12781 tgatttatga cgaaggtttg ctaggaactt tgaaaatttg tgtaaatgct tgtaaaaagg
    12841 agaatagaaa gcagttttta actatgtata aaatgtttgc taagaataaa cagaaattgg
    12901 gctttattgc gatggctagt tataaatcgt caaaacgtta gataattatt gaagttaact
    12961 tttccttttt tctttcttaa aaaatatgct ataatagaga gtaaaaaact ttgaaagaaa
    13021 gaaaaaagat gaatttaaaa gattacattg caacaattga aaattatcca aaggaaggca
    13081 ttaccttccg tgatattagt cctttgatgg ctgatggaaa tgcttatagc tacgctgttc
    13141 gtgaaatcgt tcagtatgct actgacaaga aagtcgacat gatcgtggga cctgaagctc
    13201 gtggatttat cgtgggttgt ccagttgcct ttgagttggg aattggtttt gcgcctgttc
    13261 gtaagccagg taaattgcca cgcgaagtta tttctgctga ctatgaaaaa gagtacggtg
    13321 tcgatacctt gactatgcac gcggatgcca ttaagccagg tcaacgtgtt cttattgtag
    13381 atgacctttt ggcgacaggt ggaactgtta aggcaactat cgagatgatt gaaaaacttg
    13441 gtggtgttat ggcaggttgt gccttccttg ttgaattgga tgaattgaac ggccgtgaaa
    13501 aaatcggtga ctacgactac aaagttctta tgcattatta atgaaaacag tccctagggc
    13561 tgttttctct acactaggat ataaaaatag actataacta gttagagaaa aactataatt
    13621 gaaaactata tcttcttgca gtataataaa aggactaagt gtttgagatt tgtcttcaaa
    13681 cacatgcaat tattcctgag agagtacagt taggagaggg ttatgccgat tcgaattgat
    13741 aaaaaattgc cagctgttga gattttacgg acagagaata tctttgtcat ggatgatcaa
    13801 cgtgctgccc accaagatat ccgtcctttg aagattttaa ttttaaatct catgccacag
    13861 aaaatggtca cagagaccca gttgttgcgc cacttggcta atacacccct acaactggat
    13921 attgattttc tctatatgga gagccaccgt tctaaaacaa ctcgttcaga gcacatggag
    13981 accttctata aaacttttcc tgaagtcaag gatgagtatt ttgatgggat gatcatcacg
    14041 ggtgctccag ttgagcattt accatttgag gaagtggact attgggagga atttagacag
    14101 atgcttgagt ggtctaagac tcatgtctat tcgacccttc atatctgttg gggagctcag
    14161 gctgggcttt atctgcgcta tggtgtagaa aaataccaga tggacagtaa gctatcaggt
    14221 atttatcctc aggacaccct aaaagagggt caccttctat ttagaggctt tgatgatagc
    14281 tatgtatccc ctcattcacg gcacacggag atttctaagg aagaggtctt aaacaagact
    14341 aatctcgaga ttttatcaga aggacctcag gttggggttt ctattttggc cagtcgtgat
    14401 ttacgagaaa tttatagttt tggtcatttg gagtatgacc gtgatacctt ggcaaaagag
    14461 tattttcgag atcgtgatgc aggtttcgac ccacatattc cagaaaatta ctttaaagat
    14521 gatgatgtta atcaggtacc ttgtctttgt tggtcttcat ctgcagccct ctttttcagt
    14581 aattgggtaa accatgcggt ctatcaggag acgccttttg actggagaaa gatagaagat
    14641 gatgcatctg catatgggta tttataagag gaattatgac atatttagac gcttttaaat
    14701 caggtacctt ggttttaccg agtgccctgc tcttgcattt taaggaactc tttccttcta
    14761 gcgacgattt tctggtttgg caatttttct atttgcaaaa tacgacaggc ttagaagaaa
    14821 tgtcgccaag ccagattgct gaaaggattg gcaaggaaat ttcggatgtc aaccagtcca
    14881 tttctaatct gacggaaagg ggactgctcc agtatcgtac tatcgaatta aatggcgaaa
    14941 ttgaattgct ctttgatgct agtttggcct tggaacgttt ggatgacctg tttggagcag
    15001 ttcattcaag ttcagaccag ctaacaccac aaaaccagct caaggatttg gtggaaacct
    15061 tccagcagga gttgggacga ttgttgacgc cttttgagat tgaggatttg accaagacac
    15121 taaaggaaga tggaaccagt gctgacttga ttaaggaggc tcttcgtgaa gctgttttga
    15181 atggaaaacc aaactggaag tacattcagg cgattttgag aaactggcgc catgaaggaa
    15241 tcaagagtgt ggctcaaatt gaggccaaga gagcagaaag agaagcaagc aatcctcagt
    15301 tgacacaggt atctgcagat ttcagaaatg ccatggatct ctggaaggat taatccatgc
    15361 aagtaggctt gaaatccgag taagatttgc aagctgtgta taattgtgat agaataaata
    15421 gaaaataaat tgaaaaaaga ggtatgtgaa atgtcacgta aaccatttat cgctggtaac
    15481 tggaaaatga acaaaaatcc agaagaagct aaagcattcg ttgaagcagt tgcatcaaaa
    15541 cttccttcat cagatcttgt tgaagcaggt atcgctgctc cagctcttga tttgacaact
    15601 gttcttgctg ttgcaaaagg ctcaaacctt aaagttgctg ctcaaaactg ctactttgaa
    15661 aatgcaggtg ctttcactgg tgaaactagc ccacaagttt tgaaagaaat cggtactgac
    15721 tacgttgtta tcggtcactc agaacgccgt gactacttcc atgaaactga cgaagatatc
    15781 aacaaaaaag caaaagcaat ctttgcgaac ggtatgcttc caatcatctg ttgtggtgaa
    15841 tcacttgaaa cttacgaagc tggtaaagct gctgaattcg taggtgctca agtatctgct
    15901 gcattggctg gattgactgc tgaacaagtt gctgcctcag ttatcgctta tgagccaatc
    15961 tgggctatcg gtactggtaa atcagcttca caagacgatg cacaaaaaat gtgtaaagtt
    16021 gttcgtgacg ttgtagctgc tgactttggt caagaagtcg cagacaaagt tcgtgttcaa
    16081 tacggtggtt ctgttaaacc tgaaaatgtt gcttcataca tggcttgccc agacgttgac
    16141 ggtgcccttg taggtggtgc gtcacttgaa gctgaaagct tcttagcttt gcttgacttt
    16201 gtaaaataat cagtaagtag caaaagctag gtggaacagc attcagatgt ctgttacatt
    16261 ttttatagga gagcaaagat tgaaaacaaa aattggatta gcaagtatct gtttactagg
    16321 cttggcaact agtcatgtcg ctgcaaatga aactgaagta gcaaaaactt cgcaggatac
    16381 aacgacagct tcaagtagtt cagagcaaaa tcagtcttct aataaaacgc aaacgagcgc
    16441 agaagtacag actaatgctg ctgcccactg ggatggggat tattatgtaa aggatgatgg
    16501 ttctaaagct caaagtgaat ggatttttga caactactat aaggcttggt tttatattaa
    16561 ttcagatggt cgttactcgc agaatgaatg gcatggaaat tactacctga aatcaggtgg
    16621 atatatggcc caaaacgagt ggatctatga cagtaattac aagagttggt tttatctcaa
    16681 gtcagatggg gcttatgctc atcaagaatg gcaattgatt ggaaataagt ggtactactt
    16741 caagaagtgg ggttacatgg ctaaaagcca atggcaagga agttatttct tgaatggtca
    16801 aggagctatg atgcaaaatg aatggctcta tgatccagcc tattctgctt atttttatct
    16861 aaaatccgat ggaacttatg ctaaccaaga gtggcaaaaa gtgggcggca aatggtacta
    16921 tttcaagaag tggggctata tggctcggaa tgagtggcaa ggcaactact atttgactgg
    16981 aagtggtgct atggcgactg acgaagtgat tatggatggt gctcgctata tctttgcggc
    17041 ctctggtgag ctcaaagaaa agaaagattt gaatgtcggc tgggttcaca gagatggtaa
    17101 acgctatttc tttaataata gagaagaaca agtgggaacc gaacatgcta agaaaatcat
    17161 tgatattagt gagcacaatg gtcgtatcaa tgattggaaa aaggttattg atgagaacga
    17221 ggtggatggt gtcattgttc gtctaggtta tagcggtaaa gaagacaagg aattggcgca
    17281 taacattaag gagttaaacc gtctgggaat tccttatggt gtctatctct atacctatgc
    17341 tgaaaatgag accgatgctg agaatgacgc taaacagacc attgaactta taaagaaata
    17401 caatatgaac ctgtcttacc ctatctatta tgatgttgag aactgggaat atgtaaataa
    17461 gagcaagaga gctccaagtg atacagacac ttgggttaaa atcatcaaca agtacatgga
    17521 cacgatgaag caggcgggtt atcaaaatgt gtatgtctat agctatcgta gtttattaca
    17581 gacgcgttta aaacacccag atattttaaa acatgtaaac tgggtagcgg cttatacgaa
    17641 tgctttagaa tgggaaaacc cttattattc aggagaaaaa ggttggcaat atacctcttc
    17701 tgaatacatg aaaggaatcc aagggcgcgt agatgtcagc gtttggtatt aagcgatgat
    17761 ttgaaagagg gatgtgatag tagcaccctc tttttctttg ttttatgata gttcatcctc
    17821 gagtaaattc aagatcttgc tcggaaatga agcttatata gtagattgaa tatagacaaa
    17881 taccttgtga ttggtaaaac attttagaaa ttcatttacc tttcctaatc gacttggttt
    17941 catcttattt caatctatta tagtattggg gaatttcttc aaaccacatc agcttggtca
    18001 gttctacctg cgacctcaaa acttgtgctt tggtcaagct gggtttagtt tcctagtttg
    18061 ctgatggatt tccattgact ataagcatcc aaccctcttt ttgtcttcta aagaattctt
    18121 aaattatcag tctattgcaa cttttctcat gtgggtcatt tggcttgcaa ttgtctttcc
    18181 ttagtagtat actgaggtag taatcattaa gaagtggtta cataaaaata atgaatgagg
    18241 taaaataaaa tggtagaatt gaaaaaagaa gcagcaaaag atgtcgcaac attaacaaaa
    18301 gcagcgccag tagcattggc aaaaacaaag gaagttttga atcaagcagt tgctgatttg
    18361 tatgtagctc atgttgcttt gcaccaagtg cactggtata tgcatggtcg tggtttcctt
    18421 gtatggcatc caaaaatgga tgagtacatg gaagctcttg atggtcattt agatgaaatc
    18481 agcgaacgct tgatcacact tggtggaagc ccattctcta ctttgacaga gttccttcaa
    18541 aacagtgaaa tcgaagaaga agctggtgaa taccgtaatg ttgaagaaag cttggaacgt
    18601 gttctagcta tttatcgcta cctcatcact cttttccaaa aagcgttgga tgtgactgac
    18661 gaagaaggcg atgatgttac aaacgatatt tttgttggtg ctaaggctga acttgagaaa
    18721 acagtttgga tgcttgccgc agaacttgga caagcacctg gtttgtaaga aacaagacaa
    18781 catagataaa tctgtaggaa atttcttaca gattttttct attttgtaag ctattccaaa
    18841 ccagtctttt tttgagtttt ttgataaaat agtactatca gtgaaaagga tggaagcatg
    18901 actaagaaaa tcgtagctat ttgggcccag gatgaagagg gtgtgattgg taaggacaat
    18961 cgtctgcctt ggcatttgcc agcagaattg cagcacttta aagaaacaac tctgaatcat
    19021 gctatcttga tggggcgggt aacctttgat ggaatggggc gtcgcttgct tccaaaacgg
    19081 gagaccctga ttttgacgcg taatccggaa aaaaagatag atggggttgc tacttttcag
    19141 gacgtccagt ctgttcttga ctggtatcag gctcaagaaa agaatctcta cattatcggt
    19201 gggaagcaaa tttttcaggc ttttgaagct taccttgatg aagtgattgt cactcacatt
    19261 catgctcggg tggaaggaga tacctatttc cctgaagagc ttgacttgtc tctttttgag
    19321 acagtttcaa gcaaatttta cgccaaagat gagaagaatc cttatgattt taccatccaa
    19381 taccgcaaga gaaaggaagt ttaatggaac ggagtttatt tggtctattc acggcttttc
    19441 tgtgtttcat ctgctttctg gcaggagcac aagcttttcg taaaaagcgt tacgggcttt
    19501 ctatcctcct ttggttaaat gcctttacca atctggtcaa tagtattcat gctttttaca
    19561 tgaccttatt ttagatagaa tgacagtata gaatggaagg aaatcatgtc tacaaataga
    19621 aaaaatgata tgatggttta ttgctcattt tgtggcaaaa accaagaaga agtacaaaaa
    19681 ataattgctg gcaacaatgc ttttatttgt aatgaatgcg tggagttagc tcaggaaatc
    19741 attcgagaag aattggttga ggaagtcttg gcagacttgt ctgaggtgcc aaaaccaatt
    19801 gaactcctcc atatcttgaa ccactatgta attggtcaag atcgtgccaa gcgtgccttg
    19861 gcagtggcgg tttataacca ctacaaacgc atcaatttcc acgatacacg cgaagagtca
    19921 gaagatgtgg atttgcagaa gtcaaacatt ttgatgattg gcccaactgg ttcagggaaa
    19981 actttccttg cccagacctt ggctaagagc ttgaatgtac cttttgctat tgcggatgcg
    20041 acagctctga cggaggctgg ttatgtgggt gaggatgtgg aaaatatact cctcaaactc
    20101 ttgcaggttg ctgactttaa catcgaacgt gcagagcgtg gcattatcta tgtggatgaa
    20161 attgacaaga ttgccaagaa gagtgagaat gtgtctatca cacgtgatgt ttctggtgaa
    20221 ggggtgcaac aagcccttct caagattatc gagggaactg ttgctagcgt accgcctcaa
    20281 ggtggacgca aacatccaca acaagagatg attcaagtgg atacaaaaaa tatcctcttc
    20341 atcgtgggtg gtgcttttga tggtattgaa gaaattgtca aacaacgtct gggtgaaaaa
    20401 gtcatcggat ttggtcaaaa caataaggcg attgacgaaa acagctcata catgcaagaa
    20461 atcatcgctg aagacattca aaaatttggt attatccctg agttgattgg acgcttgcct
    20521 gtttttgcgg ctcttgagca attgaccgtt gatgacttgg ttcgcatctt gaaagagcca
    20581 agaaatgcct tggtgaaaca ataccaaacc ttgctttctt atgatgatgt tgagttggaa
    20641 tttgacgacg aagcccttca agagattgct aataaagcaa tcgaacggaa gacaggggcg
    20701 cgtggacttc gctccatcat cgaagaaacc atgctagatg ttatgtttga ggtgccgagt
    20761 caggaaaatg tgaaattggt tcgcatcact aaagaaactg tcgatggaac ggataaaccg
    20821 atcctagaaa cagcctagag gtgactatgg aacttaatac acacaatgct gaaatcttgc
    20881 tcagtgcagc taataagtcc cactatccgc aggatgaact gccagagatt gccctagcag
    20941 ggcgttcaaa tgttggtaaa tccagcttta tcaacactat gttgaaccgt aagaatctcg
    21001 cccgtacatc aggaaaacct ggtaaaaccc agctcctgaa cttttttaac attgatgaca
    21061 agatgcgctt tgtggatgtg cctggttatg gctatgctcg tgtttctaaa aaggaacgtg
    21121 aaaagtgggg gtgcatgatt gaggagtact taacgactcg ggaaaatctc cgtgcggttg
    21181 tcagtctagt tgaccttcgt catgccccgt cagcagatga tgtgcagatg tacgaatttc
    21241 tcaagtatta tgagattcca gtcatcattg tggcgaccaa ggcggacaag attcctcgtg
    21301 gtaaatggaa caagcatgaa tcagcaatca aaaagaaatt aaactttgac ccgagtgacg
    21361 atttcatcct cttttcatct gtcagtaagg cagggatgga tgaggcttgg gatgcaatct
    21421 tagaaaaatt gtgaggaaaa gaaaatggca aaaacaattc atacagataa ggccccaaag
    21481 gctatcggac cctatgttca aggaaaaatc gttggcaacc ttttgtttgc tagcggtcaa
    21541 gttcccctat cccctgaaac tggggaaatt gtaggagaga atatccaaga acagacagag
    21601 caagtcttga aaaacatcgg tgctattttg gcagaagcag gaacagactt tgaccatgtt
    21661 gtcaaaacaa cttgcttctt gagcgatatg aacgactttg ttccttttaa tgaggtttac
    21721 caaacggcct tcaaagagga attcccagct cgttcagctg tggaggtagc tcgtcttcct
    21781 cgtgatgtaa aagtcgaaat tgaagtcatc gcagagattg gataagctag ttgaagtttg
    21841 gtgttgccaa acttcttttg atataaggag aaaaagatga caaagaaaca acttcacttg
    21901 gtgattgtga cagggatgag tggcgcaggg aaaactgtag ccattcagtc cttcgaggat
    21961 ctaggttatt tcaccattga taatatgccg ccagctctct tgcctaagtt tttgcagctg
    22021 gttgaaatta aggaagacaa tcctaagttg gccttggtag tggatatgcg tagccgttct
    22081 ttcttttcag agattcaagc tgttttggat gagttggaaa atcaagatgg tttggatttc
    22141 aaaatcctct ttttggatgc ggctgataag gaattggtcg ctcgttacaa ggaaaccaga
    22201 cggagtcacc cactagcagc agacggtcgt attttagatg gaatcaagtt ggaacgtgaa
    22261 ctcttggcac ctttgaaaaa tatgagccaa aatgtggtgg atacgactga actcactcca
    22321 cgtgagctgc gtaaaaccct tgcagagcag ttttcagacc aagaacaagc ccagtctttc
    22381 cgtatcgaag tcatgtcttt cggatttaaa tatggaatcc cgattgatgc ggacttggtc
    22441 tttgatgtcc gtttcttgcc aaatccctat tatctaccag aactgagaaa ccaaacgggt
    22501 gtggatgaac ctgtttatga ttatgtcatg aaccatcctg agtcagaaga cttttatcaa
    22561 catttattgg ccttgattga gccgattctg ccaagttacc aaaaggaagg taagtccgtt
    22621 ttgaccattg ctatgggatg tacgggtgga caacaccgta gtgtggcatt tgctaaacgc
    22681 ttggtgcagg acttatccaa gaattggtct gttaatgaag ggcatcgcga caaagaccgc
    22741 agaaaggaaa cggtaaaccg ttcatgagaa aaccaaagat aacggtgatt ggtggaggga
    22801 ctggaattcc cgtcattcta aaaagtctgc gggaaaaaga tgtggaaatc gcagctatcg
    22861 tgacggtggc agatgatggt ggttcttcag gtgaactccg aaaaaatatg caacagttga
    22921 caccgccagg tgatcttcgt aatgtccttg tggccatgtc ggatatgcct aagttttatg
    22981 agaaggtctt tcagtatcgg ttctctgagg atgccggagc ctttgctggc catccattgg
    23041 gaaatctcat cattgctggc ttgtcagaaa tgcagggttc aacctataat gccatgcagt
    23101 tattgagcaa atttttccat acaacaggga aaatttatcc ttccagtgac catcctttga
    23161 cccttcatgc agtctttcag gatgggacag aagtggctgg agagagtcat attgtagacc
    23221 atcgaggcat aattgacaat gtctatgtga ccaatgccct aaacgatgat acgcctctgg
    23281 ccagccgtcg agtagtgcag accatccttg aaagtgacat gattgtccta gggccaggtt
    23341 ccctctttac ctctattttg cccaatatcg tgattaagga aattgggcgg gctcttttgg
    23401 aaactaaggc agaaattgcc tatgtctgca atatcatgac ccaacgtggg gagacggaac
    23461 actttacaga tagcgaccac gtggaagtct tgcatcgtca ccttggtcgc ccttttatcg
    23521 acactgtctt ggtgaatatt gaaaaagtgc ctcaggaata catgaattcc aaccgttttg
    23581 atgaatactt agtgcaagtg gaacacgatt ttgtaggtct ttgtaagcaa gtttcgcgcg
    23641 tgatttcatc taacttcctt cgtctggaaa atggcggtgc cttccacgat ggagatttga
    23701 ttgtggacga gttgatgcgc attatacagg tgaaaaaatg agtttcacag tagcagtaaa
    23761 agaagaaatc ctgggccaac accatctgag ctggcatgaa ttatctgcca ttatcaaaat
    23821 gtctggtagc atcggtctct cgacttcggg cttgactttg tcagttgtga cagaaaatgc
    23881 caaactggcc cgtcacctct atgagtcctt tctccatttc tatgaaatca aatctgaaat
    23941 tcgtcaccac caacggagca atcttcgcaa gaatcgcgtc tataccgttt ttacagatga
    24001 aaaggtgcag gatttgttaa gtgatttgca cttggcagac tctttctttg gcctggaaac
    24061 aggtattgat gaggcgattt tatcggacga ggaagcaggt cgtgcctatc tctgtggcgc
    24121 tttcttggca aatgggagca ttcgtgaccc tgagtcaggc aagtaccagt tggaaatcag
    24181 ttctgtttat ctggaccacg cgcaaggaat tgcctccctt ctccaacagt ttttactgga
    24241 tgccaaggtg cttgagcgca agaagggggc tgtgacctat ctccagcgag cagaagacat
    24301 tatggacttc ttgatagtaa taggagccat gcaggcacgt gatgattttg agcgggttaa
    24361 gattttgcga gaaacccgta acgacctcaa tcgggctaat aatgccgaga cagctaatat
    24421 cgctcggaca gtttctgcca gcatgaagac cattaacaat atcagtaaaa tcaaagatat
    24481 catgggctta gaaaatttgc cagtagattt gcaggaagtg gcgcaactga ggattcagca
    24541 cccagactac tctatccagc agttagcaga tagtctcagc acccctctga ccaaaagtgg
    24601 tgtcaatcac agactcagaa aaatcaataa gatagcggat gaattataaa accacgaatc
    24661 ctatgtgact cgtggttctt ttttataaac tggtagagtg ttttggttgt actttttgtt
    24721 cagggtcaat gtagttgata gcgttgttga cagcagttgg agcttctccg aggcctgtcg
    24781 caatcagatc aatttttccg tcatagtagc agcagtcacc gatagcatag atacctgctt
    24841 ggctggattc ctgtttgctg ttgacgataa tcttgtgacg gttgaggtcg agcccccagt
    24901 ttttaaggtt accgacagaa gatttgaaac catagttgac aaagaggtgg tctaggtcaa
    24961 tggtttcagt ttcatcagat ttgacttttg tgatttcaag tttatcaagt gtttttccat
    25021 ttccaaggag ttggctaggg gcgaatggtg tcttgatggt tacagatgat tcttgcaagg
    25081 tttgaacact gtgttccaag gcacggaaat tatctctgcg gtgaacaagg gtagttggtg
    25141 cgattttttc aaaagccaaa gcccaatcca cagccgagtc tcccccacca agaatcgtca
    25201 ctttcttacc agcgtattgc tgaatgttag aaacgtggta gtggatattt tcatagtcct
    25261 caaccccttc aagttccagc ggacgtggtt tgaaggcacc gccacccata gcgatgataa
    25321 ctgttttagt caggtgactt cctttggaag ttgtgatggc aaattcttct tcttgtttgt
    25381 caatctcaag aaccgtttca ttgagatgaa taggggtatc aaatccattt agctgttcaa
    25441 tcaagcggtt agtcaactct tctccagtca ggtttgggaa gcctggtacg tctaggattt
    25501 ccttttcagg gtagagaata gcaggttgtc cacctagctg gggaagagag tcgatgattt
    25561 gaaccttggc ttggcgtagg tgggcataaa aggctgcaaa aagcccgaca ggaccaccac
    25621 ccacaatggt aatatcatag agttgagaca tagtttctcc tttgtttttt ctagtcagtt
    25681 tattttatca tatttttgtt caatgtaaaa tggactagga tagggaaaaa aatgccagaa
    25741 aatggtataa taggacgaaa gtgttttgat agggaggata tcagggatga attttcaaca
    25801 attatccaat ctgcaatatt ggaccagttt gtttgcaagt ccatggacga tagctatcaa
    25861 tctgattgat attttgattg ttgcttatat tttataccat tttacaaaag ctattgcagg
    25921 aaccaagatt atgattttgg tacgtggagt tttggtgttt attttagctc aaatccttgc
    25981 aaatatgatt ggtttgacta cgatttcttg gttaatcaat caaattatta cttatggggt
    26041 tattgcggcg gttgttatct tctctccaga gattcggact ggtttggaac gtttgggaag
    26101 agcgacagat ttcttttcca atgcccctat tagtgctgag gaacagatga ttcgtgcctt
    26161 tgttaagtct gttgaataca tgagtcctcg taaaatcggg gccttggttg ctattcagcg
    26221 tgtacgtacc ttgcaggagt atatgtcgac aggaattccc ttggatgcta agatttctgc
    26281 agaacttctc attaacattt ttattcccaa cactccccta catgacggtg cggtgattat
    26341 caaagaagaa cgtatcgctg tgacgtctgc ctatctgccc ttgacaaaaa acacagggat
    26401 ttccaaggaa tttgggacca gacaccgggc ggctatcggt ttatcagaag tctcagatgc
    26461 cttgactttt gtcgtatcag aggaaacggg aggaatttcg ataacctata atggaaggtt
    26521 taagcacaac ctaacacttg atgaatttga aacagaatta cgtgaaatct tacttccaaa
    26581 agaggaagtg ggtcttagtt ttaaagaacg attgctagga ggatggaaac atgaaaaaaa
    26641 atagtttata tatcatatcc tcactctttt ttgcttgtgt cttatttgtc tatgctacgg
    26701 cgacgaattt tcaaaacagt accagtgcta ggcaggtaaa aacggaaacc tatactaata
    26761 cagtaaccaa tgtccctatt gacatacgct ataatagtga taagtatttt attagcggtt
    26821 ttgcttcaga agtatcagtg gtcttgactg gtgcaaatcg cctatcgcta gctagtgaaa
    26881 tgcaagaaag tacacgtaaa ttcaaggtta ctgctgacct aacagatgcc ggtgttggaa
    26941 cgattgaagt tcctttgagc attgaagatt tacccaatgg gctgaccgct gtggcgactc
    27001 cgcaaaaaat tacagtcaag attggtaaga aggctcagaa ggataaggta aagattgtac
    27061 cagagattga ccctagtcaa attgatagtc gggtacaaat tgaaaatgtc atggtgtcag
    27121 ataaagaagt gtcgattacg agtgaccaag agacattgga tagaattgat aagattatcg
    27181 ctgtcttgcc aactagcgaa cgcataacag gtaattacag tggttcagta cctttgcagg
    27241 caatcgatcg caatggtgtt gtcttaccgg cagttatcac tccgtttgat acaataatga
    27301 aggtgactac aaaaccagta gcaccaagtt caagcacatc aaattcaagt acaagcagtt
    27361 catcggagac atcttcgtca acgaaagcaa ctagttcaaa aacgaattaa aaatagaaaa
    27421 ggattttata acaatgggta aatattttgg gactgatgga gtccgtggag aagctaacct
    27481 agagctaaca ccagaattag cctttaaact aggacgtttt ggaggctatg ttcttagtca
    27541 acatgaaacg gaagcgccga aagtctttgt aggacgtgac acacgtattt caggggaaat
    27601 gctggaatcg gccttggtgg caggtctcct ttcagtaggg attcacgtat acaaacttgg
    27661 tgtccttgca acaccagcag tagcttactt ggttgaaact gaaggagcaa gtgccggtgt
    27721 catgatttct gctagccaca acccagccct tgataacgga atcaagttct ttggcggtga
    27781 tggcttcaaa ctagatgatg aaaaagaagc agaaattgaa gccttgctag atgctgagga
    27841 agacactctt cctcgtccaa gtgcagaagg cttaggaatt ttggtagatt atccagaagg
    27901 cttgcgtaag tatgaaggat accttgtttc aactggaact cctcttgatg gaatgaaggt
    27961 tgccttggat acagctaatg gagcagcttc taccagtgcc cgtcaaatct ttgcagacct
    28021 tggtgcccaa ttgacggtta tcggggaaac accagacggt cttaacatca accttaatgt
    28081 tggttcaaca catccagaag cccttcaaga agtggtcaaa gaaagtgggt cagctattgg
    28141 tttggccttt gatggagaca gtgaccgctt gattgctgtt gatgagaatg gtgacatcgt
    28201 cgatggtgac aagattatgt acatcatcgg aaaatacctt tctgaaaaag gacaattggc
    28261 tcaaaataca attgtgacaa ctgttatgtc taaccttggt ttccacaagg ccttgaatcg
    28321 cgaaggtatt aacaaggcag ttactgcagt tggtgaccgc tacgttgttg aagaaatgag
    28381 aaaatcaggt tacaaccttg gtggtgaaca gtctggtcac gttatcttga tggattacaa
    28441 taccacaggt gatggtcaat tatcagcagt tcaattgact aaaatcatga aggaaactgg
    28501 taagagctta tcagagttgg cggcagaagt aacgatttat ccacaaaaat tagttaatat
    28561 ccgagtggaa aacgtcatga aggaaaaggc catggaagtg ccagctatca aggccatcat
    28621 cgagaagatg gaagaagaaa tggcggggaa cggccgtatc cttgttcgtc caagtggaac
    28681 agaacccctc ttgcgtgtta tggcagaagc gcctacaaca gaagaagtag actactatgt
    28741 tgataccatc acagatgtag ttcgtgctga aattgggatt gactaaatcc tagaaaacta
    28801 gatgaaaata aaaaattatg gtacaatagc ttataatatt gtagccgatg gagaaagaca
    28861 tgaatcttaa acgtgaacaa gaatttgtta gtcagtatca ttttgatgcg cgtaattttg
    28921 aatgggaaaa tgaaaatgga gctcctgaaa ccaaggtgga tgtgaatttc caattgctcc
    28981 aacatgacca agaaaatcaa gtgacttcct tgattgtcat cttgagcttt atgattgtat
    29041 ttgataagtt tgtcattagt ggaacaatct cacaagtaaa ccatatcgat ggccgtattg
    29101 tcaatgaacc aaatgaattg aaccaagaag aggtggaaac cttggctcgt ccatgtttga
    29161 acatgctcaa tcgtttgact tacgaagtga ctgaaattgc attagactta ccaggaatta
    29221 atttggagtt ctaatatgaa attagctgtt ttcacagatt cctctgctta tctcagtgca
    29281 gagatcttgc aaagagaaga cttgtttgtc ttggatattc ctgtcaatat tgatggtgag
    29341 gaatatgtcg aaggcatcaa tctgtctgct gaggaatttt accaaaaaat ggctcaggct
    29401 tctgaattgc ctaagaccag tcaaccaagt attgccaagt tagatgagat cttaacttcg
    29461 ctcaaagaac aaggctatac acatgccttg gggcttttcc tatcttctgg aatttcaggt
    29521 ttttaccaaa atatccagta tatggtcgat gactatgagg gcttaaccat tgctttccca
    29581 gacactttga ttacaagtgc tcccctaggc atcatggttg aaagtgtctt taattggcgt
    29641 gatcagggcg atgattttgc cagcattcag gataagctag ccattcaaat cagccgtacg
    29701 tcagccttta tcatggtaga tgacttggat catttggtaa aaggtggacg cctttcaaat
    29761 ggggctgcca ttttgggcaa tttgcttagc attaagccaa tcctttattt taacgatcaa
    29821 ggtgtgattg aagtttacga aaaagttcgt actgaaaaga aggccaccaa gcgcttaatt
    29881 gaaattatca aggaaacaac ggcttcaggt caataccggg tcattgtcat tcatgggaat
    29941 gctcctgaaa aggctgaaga attgcgtcag cacttgcttg attttggctt gggttcggat
    30001 gtttcacttg ctacatttgg tagtgtcatt ggaacgcacc taggagcagg aagtattgct
    30061 ctgggttata ttccagtgat ttagttggcg cttctaggct agttaagatg ccttgtatct
    30121 tagaaattga acacggacta cctgcctctt gaaaaaagat gcctgtcttg cctttcgtgg
    30181 aaagtcagtg ccattcccta tttttcagag gcagctaagg tcctttgtat cttgataatt
    30241 gaacacgcct ggaaccctgt gtgaaaaaga tagttcttcc aagaagtaga cacttcttga
    30301 ttggaattcc tattttcact ttgtgttctt acggactttg tatcttagac aggagtagag
    30361 atgagtattc gagtaattat tgccggtttt aagggaaaga tgggccaggc tgcttgtcag
    30421 atggtattga ctgatccaga cttggacttg gtggcagttt tggatccttt tgagtctgag
    30481 tcagaatggc agggtattcc tgttttcaag gataaggctg atttagctgg ttttgaagcg
    30541 gatgtctggg tagattttac tactccagct gttgcctacg aaaatacacg ttttgctctt
    30601 gaaaatggct ttgctccagt agttggaacg actggtttca cgagtgaaga aattgcagag
    30661 ctaaaagaat tttctcgtgc ccaagacttg ggtggcctga ttgcccctaa ctttgccttg
    30721 ggtgctgtct tactcatgca atttgcgacg caggctgcca aatatttccc aaatgtggag
    30781 attattgagc tccatcatga caagaaaaag gatgctccga gtggaacagc cattaaaaca
    30841 gctgagttga tggcagaggt tcgagagtcc attcagcaag gtgcagcaga tgaggaagag
    30901 ctgattgctg gtgctcgtgg tgctgacttt gatggtatgc gcatccactc agttcgtttg
    30961 ccaggcttgg tagctcatca ggaagtcatc tttggcaatc agggagaagg gttgaccctc
    31021 cgtcatgact cctatgatcg catctccttc atgacaggag tcaatttggg aattaaagaa
    31081 gttgtcaagc gtcatgagct tgtctatgga ttagaacact tattatgaga ttaatgcaaa
    31141 tgccttctga atttcagaag gctttaccag tattagaaaa aattaaagaa gcaggctttg
    31201 aggcttattt tgttgggggc tctgttcgag atgcccttct ccatagccct atccacgatg
    31261 tggatattgc gacgtcttct tatccagaag agaccaagca gatttttccg cgaacagccg
    31321 atatcggaat cgagcatgga accgtcttgg tcttagatgg ggatgaggag tatgaggtaa
    31381 caacctttcg gacagaggat gtctatgtgg actatcgcag acccagtgcg gtttcctttg
    31441 tgcgctcgct agaagaagac ctcaaacgcc gtgatttcac agtcaacgcc tttgccttgg
    31501 atgagacagg agaaatcgtt gacttgttcc atggtttaga agatttggaa aagcaagtct
    31561 tgcgagcagt tggagtggct agtgagcgtt tcaacgaaga tgctttacgg attatgcgtg
    31621 gtttccgttt tcaggctagt cttggttttg cacttgagcc agaaacattt aaagctatga
    31681 agaccttgac gccgcttttg gagaaaattt ctgtagagcg taccttcgtc gagtttgata
    31741 aactcttgct ggctccattt tggagaaggg gcttggcttc catgattgag agtcaagctt
    31801 atgactatct ccctgatatg gcatctagcc aggacaagct caacagactg tttgatttgg
    31861 agactgattt tacttttgaa tcctctgaac aagcctgggc ggctttacta tgggctttgg
    31921 agattgaaaa tgcgcagtca tttttgaaat cttggaagac ctcacgccag tttgccaagc
    31981 aagttcagga tttgctgatt attttggctc tgcgtgaaaa tggagaattg agtaagcgtg
    32041 attgttatcg ctttgacttg gatttacttt tacaggctga aaatcttcgt caagctcagg
    32101 gaaaagaagt caacccacaa gtcattaaag aaacctacca gagcttgact attcatgata
    32161 agaaagaaat ccaaatcaac ggcggtattt tgatcaagga atatggctat cagccaggcc
    32221 cagacttggg agagatttta acagagattg agtttgccat tgtcgatgga gaattggaga
    32281 ataatcgtga agccatccat gcttacctga gggagaaaaa atgagtgatt ttatcgttga
    32341 aaaactaagt aaatccgttg gtgacaagac cgtttttagg gatatttcct ttattatcca
    32401 tgacttagac agaattggtt taatcggtgt caatgggact ggcaagacca cccttttgga
    32461 cgtcctttct ggtgtttctg gatttgatgg ggatgtcagt cctttttcag ctaaaaatga
    32521 ttaccagatt ggttacttga ctcaggatcc tgattttgat gatagaaaga cagttttgga
    32581 tacggttcta tctagtgaac tcaaggaaat ccagctcatt cgtgagtatg aattgattat
    32641 gctcgactat agtgaggaca agcaggcgcg tttggaacgt gtcatggcag agatggactc
    32701 tctccaagct tgggaaattg agagtcaggt caagaccgtt cttagcaaat tgggcattca
    32761 agacttatct actcctgttg gggaattgtc aggtggtctg agaagacggg tacagttggc
    32821 acaagtctta cttggcaacc acgacctttt gcttttggat gagccgacca accatctgga
    32881 tattgcgatt attgagtggc tgaccctctt tttgaaaaat tctaagaaga ccgtcctttt
    32941 tatcactcac gatcgttatt tcttagacgc tttgtcaaca cggattttcg agttggagcg
    33001 agcaggcttg acagaatatc agggcaatta ccaggactat gttcgcctta aggcagaaca
    33061 ggacgagcgc gatgcagctc tacttcataa aaaagaacaa ctctacaaac aagaactggc
    33121 ctggatgcgc agacaaccgc aggcacgtgc gaccaagcaa caagctcgta tcaatcgttt
    33181 ccatgatctg aaaaaggaag tttcaggcag tagtgctgag acagacttga ctatgaactt
    33241 tgaaaccagt cggattggga agaaagtcat cgagtttcag gatgtttcct ttgcctatga
    33301 aaataagccc attttgcaaa attttaatct cttagttcag gctcaagacc gtattggaat
    33361 tgttggggac aatggtgttg gaaaatcaac cctacttaac ctgattgcag gaagtcttga
    33421 gccgacagca ggacaagttg tgattgggga aactgttcgc atcgcctatt tctctcaaca
    33481 aattgagggt ttggatgaaa gcaagcgtgt gatcaattac ctgcaggaag tggcagagga
    33541 ggtcaagacc agtggtggtt ctacgacttc catcgctgag ttgctggagc aattcctctt
    33601 cccacgttcg acgcatggga ctttgattga gaaattgtca gggggtgaga aaaaacgtct
    33661 ttatctcctc aaactgcttt tggaaaaacc aaatgttctt cttttagacg agccaaccaa
    33721 tgacctagat attgcaactt tgacagtctt agagaatttc ttgcaaggtt ttgcaggtcc
    33781 cgttttaaca gtcagtcacg accgctattt cttggataag gtagcgacca agattctcgc
    33841 ttttgaggat ggcaagattc gtcctttctt tggtcattac accgactatc ttgatgaaaa
    33901 agcttttgaa acagatatgg ccaatcaagt gcaaaaggcc gaaaaggaaa aagtggtcaa
    33961 ggttcgagaa gacaagaaac gcatgaccta ccaagaaaag caggagtggg caagtattga
    34021 aggtgatatt gaaaccttgg aaaaacgtat cgctgctatt gaagaggaaa tgcaggctaa
    34081 cggctctgac tttggtaagc tggctactct ccaaaaagaa ttggatgaga aaaatgaagc
    34141 actccttgaa aaatacgaac gctatgagta tctcagtgaa tttgatagtt aagactattc
    34201 gaaaatcaaa gtgcaaacta tgtcggcttc acattgccga ctcaagtaca gcctgtgact
    34261 ggcttcctaa tttgcacttt gatttttatt gagtataaaa gaatagaaaa atcccgtctc
    34321 atgaaaggat ttctctattc ttttttggtt tcttgatgaa agatattttg ccaggtttcg
    34381 tggcgacgcc agatactggt atggatgata ccatctaact catagcagat gagtttggtt
    34441 ttttgactga tggaagtaat ttgaatgtcc ttgatagcag aatttaactc ttttttggtc
    34501 ttataagcct ctttatccat ctgctctcca tcttggcgaa tatagacaaa atcatcagtc
    34561 aagagttctt ctagttggtt tccttggtca atcaattgtt cacgcataag caatttttta
    34621 tagacattgt ctaaaatttc atcctcaggg ataggtgctg gttcgatatg gacatcggta
    34681 tcaaagacgc caaaacgctc ctccagcata gactcgacct gatccgcgat ttcatggctt
    34741 tcaaaaacag acaagtcagg attcatctct agtgtaatat ccaggtagat gttgctaccg
    34801 taggtgcgac ctctttgcga tttgaccttg ctgattttgg gaatttccat gatagccttt
    34861 tggtagtcct cgagcaggcg gtcgtcaaag ccatctgaaa gactaaagga agactcgatg
    34921 aagatatcat aggcagtctt caagataaag aaagtgatga tgatagcaac cagtttatcc
    34981 acaatcggat aattaaaact actagctagg atggcaatgg cggttccaag tgaggtaaca
    35041 gcgtcagaaa gattgtcctt agcagctgcc ttcagcgcct tggagttgga tttcttactg
    35101 aggcgagtat tgtagagata gaccacaaac ataatcgctg cagaaatgat tcctagagtt
    35161 gcaccaagag gatcaatgac cgtttcttcc cgactgagaa tcttttgaat ggtatctctt
    35221 agaacatcga aaccgacata gaacatgatg atagaagtga tcaagcttgc caaatcttca
    35281 atcttccaat gaccaaaacg gtggtcacgg tctgcaggct ggcgcgccat ccgaatcccg
    35341 attaagaggg ctacatttcc aatgatgtcc gatacgttat taaaaccatc ggccaccaaa
    35401 ctggatgaat gaaggagatg accagctgct aatttggctg cagacaagat caaataggtc
    35461 gaaatactga taatggctcc acgctcagct aacttgagat ttgagataga ttgcttcatt
    35521 caacttcctt tctagtcaca tagcattcta ccattatact ggttttcaag gaaaaattca
    35581 aataaggcag tttttactct ttgaaaactc cccaaaattt ggtataatgg tactactcaa
    35641 gggagtagct ggcagaaacc tgtgatagtg tcgtcattcc gaattttatg ctgaaaagta
    35701 tgctttccgg ccctatctta aacagcgaga cttgttatga ttaacaggtc tctttttgtt
    35761 tgtcgtgaaa agatatgaca gaaagagagt ctgttttgca cacaatgtca aggaggagac
    35821 acatgtcaaa agaacaaaaa cgccaagcgt tttatactca gagccctgaa gaggtcttgc
    35881 aggctgtgga tgcgaccgag caaggtttgt catcaagtga ggcggaaaag cgccttgccg
    35941 aatttgggca caatgaactc gaagaaggcg agaaacgatc aatcttggtc aaattcatcg
    36001 agcaatttaa ggatttgatg attatcatcc tagttgcggc agcaatcttg tcagtcgtga
    36061 cttctggtgg ggaagatatc gcagatgcca ttatcatcct agctgtggtt atcatcaacg
    36121 ctgcctttgg tgtttaccaa gaaggaaaag ctgaagaagc tattgaagcc ctcaaatcca
    36181 tgtctagtcc agttgcccgc gttcttcgtg atggacacat ggcagagatt gactctaaag
    36241 aattggtacc aggcgatatc gttgcccttg aagcaggtga cgtggtacca gcggacctac
    36301 gtttgataga agccaactct cttaaaattg aagaagcagc cttgacaggt gaatctgtac
    36361 cagtcgaaaa agacttgtca gtcgagcttg cgacagatgc tggtattggt gaccgtgtca
    36421 acatggcctt ccaaaactca aacgtaacct atggtcgtgg gatgggtgtt gttgtcaata
    36481 caggtatgta cactgaagtt ggtcatattg ctggtatgct tcaagatgcg gatgagactg
    36541 atacaccact caaacaaaat ttgaacaacc tttctaaggt cttgacctat gctatcttgg
    36601 tcattgccct tgttactttt gtagtgggtg tcttcattca agggaaaaat ccacttggtg
    36661 agttgttgac ttctgttgcc cttgccgttg cagccattcc agaaggactt cctgctatcg
    36721 ttaccatcgt tctttctctt ggtactcaag ttttggccaa acgacattcc atcgttcgta
    36781 agttgccagc agttgaaaca cttggttcaa ctgaaatcat cgcttctgat aagactggta
    36841 cgctgactat gaacaagatg acagtcgaaa aagtcttcta cgatgcggtt ctacatgact
    36901 cagctgatga tattgaacta ggtcttgaaa tgccactact tcgttcagtt gtcttggcca
    36961 atgatacgaa aatcgatgtg gaaggtaact tgattggtga cccaaccgaa acagccttta
    37021 tccaatatgc cttggacaag ggctatgatg tcaaaggttt cttagagaaa tatcctcgtg
    37081 tagctgaatt gccatttgac tctgaccgta agctcatgtc aacagttcac ccattgccag
    37141 atggtcgttt ccttgtagca gtcaagggtg cgccagacca actcttaaaa cgttgtcttc
    37201 ttcgtgataa ggctggggat attgctccga ttgatgagaa ggttacaaat ctcattcata
    37261 caaacaattc tgaaatggct catcaagcct tgcgtgtcct tgcaggtgct tataagatta
    37321 tcgatagtat tccagaaaat ctcacttctg aagagcttga aaatgattta atttttactg
    37381 gtttgattgg gatgattgac cctgaacgtc ctgaagccgc tgaggctgtt cgtgtggcta
    37441 aggaagcggg aatccgtcca attatgatta caggtgacca ccaagacaca gcggaagcca
    37501 ttgccaaacg tttgggaatc attgacgcaa acgatacaga aggtcacgtt ttaactggtg
    37561 ctgaactcaa tgaactgtca gatgaagaat ttgaaaaagt cgttggtcaa tactctgttt
    37621 atgcccgtgt gtctccagaa cacaaggttc gtatcgtcaa ggcttggcaa aaacaaggta
    37681 aagtcgttgc catgacaggt gatggtgtca atgacgcgcc agctctgaaa acagccgata
    37741 tcggtatcgg tatgggaatc actggtacag aagtttctaa gggggcttct gacatgattc
    37801 ttgcagatga taactttgcg actattatcg tcgcagtgga agaaggacgt aaggtcttct
    37861 caaacattca aaagactatt cagtacctac tttctgctaa tactgctgaa gtattaacca
    37921 tcttcctatc aaccttgttt ggttgggatg tcttgcagcc ggttcatctt ttgtggatca
    37981 acttggtaac ggataccttc ccagctattg ctcttggtgt tgaacctgcg gagcctggtg
    38041 tcatgaatca taaaccacgt ggacgcaagg caagcttctt ctcaggtggt gttttgagtt
    38101 ctatcattta tcaaggtgta ctccaagcag ctcttgttat gagtgtttat ggccttgcga
    38161 ttgcttaccc agttcatgtg ggtgacaatc atgctattca tgcagatgcc ctaacgatgg
    38221 cctttgcaac ccttggtttg attcagctct tccatgccta caatgtcaag tctgtctacc
    38281 aatccatctt gacagttggc ccattcaagt ctaagacctt taactggtcc atcttggtat
    38341 cctttatcct tctcatggca acaatcgttg tagaaccgct tgaaggaatc ttccacgtaa
    38401 ccaaactaga cttgtcacaa tggggaattg ttatggctgg aagcttttca atgattatca
    38461 tcgtcgaaat cgttaagttt attcaacgca aactcggttt tgacaaaaat gcgatttaag
    38521 ggaggtttcg gatgtcagca tatcaattac cgaccgtatg gcaggatgaa gctagtaatc
    38581 aaggagcttt tacggggcta aacagaccaa cagcaggtgc ccgtttcgaa caaaacttgc
    38641 caaaaggaga acaagctttt cagctttatt cactgagaac accaaatggt gtgaaggtta
    38701 ctatcttatt ggaagaatta ctagaagctg gttttaagga agcggcttac gacttgtata
    38761 agattgctat catggatggg gatcaattcg gatcagactt tgtgaagctc aatccaaatt
    38821 ccaagattcc agccttattg gaccagtcag gtactgaaga tgtaagagtc tttgagtctg
    38881 ctcatattct tctttacctt gctgagaaat ttggagcctt tttaccaagt aatcctgtgg
    38941 aaaaggtaga agttttgaat tggctattct ggcaagcagg tgcagcacct tttctaggtg
    39001 ggggatttgg acatttcttc aattatgctc ctgaaaaatt ggaatatcct attaaccgtt
    39061 ttacgatgga agtgaaacgc cagttggatt tattggataa ggaattggct cagaaacctt
    39121 atattgcagg caatgactat acgattgcag atattgctat ctggtcttgg tatggacagt
    39181 tagttcaagg aaatctttac caaggttctg caaaattctt ggatgcctca agttatcaaa
    39241 atctagtaaa atgggcagaa aaaattgcca atcgtccagc tgttaagcgt ggcttggaag
    39301 taacttatac agaaattaaa tagcaaaaaa gtcatcttcg gatggctttt tgctacagtt
    39361 gagggaaagg gtttgcagtt atcggctttt gtgctataat tgctataata tagtaaaaag
    39421 caagaggagt gtgaggaagt ggaaaagaaa attgtgaagg atatcttatt tttatctcaa
    39481 gtgtctcagc cggcaagtca ggaggacctt tatcttgcca gagatttgca ggatacactc
    39541 ttagcaaatc gtgatacctg tgttggtcta gctgccaata tgattggggt gcagaagcgc
    39601 gtgattatct ttaatcttgg cttggttccc gtggtcatgt ttaacccagt gcttctgtcc
    39661 tttgaaggat cttatgaggc agaagaaggc tgtttgtcct tggtaggtgt gagatcaact
    39721 aagcgttatg aaaccataag gcttgcctat cgtgacagca agtggcagga acagaccatt
    39781 accttgacag gcttcccagc tcagatttgc cagcatgagc tggatcactt ggaaggacga
    39841 attatttagg aggaaagcaa atgaaacgaa tagtctttga acttattttt atcgcaacga
    39901 cctggtatat ctttttaccg ccccttaacc tgaccagctg ggaatttctc ttcttcctct
    39961 gtgggcattt gttagttgtg gcaatcttat ttggctttgg caaggggata aaccttgtca
    40021 aaacggttca tgtgcgccac ggtaaggcgg aagctgcctt aaatcttgag ggtttcaaaa
    40081 tcaatcggtt agggaaaatt ctgttagctt cgattggagg aattcttctc ttggcagctt
    40141 tggtttcctt ggtaacttct agcatgtttc aggctaaaaa ttatgccaat gtagtcacgg
    40201 ttacggaaaa agactttact gaatttccta agagtgacac cagtaaggtt cctatcctag
    40261 atagaagtac tgctgaaaaa attggagacc gctacttggg ttccctaacc gataaggtgt
    40321 cgcaatacgt agcggcagat acctataccc aattgacaat tgatgggaaa ccttatcggg
    40381 tcacaccact agaatatgca gaccctatca aatggtttaa caatcaagcc aagggaattg
    40441 gtgagtatat caaggtggac atggtaactg gaaatgcgga tttggtggac ttgaagacac
    40501 caatcaagta ttcagactcg gagtatttta accgtgatgt caaacgtcac ctgcgcttga
    40561 agtacccgac caaaatcttt aaaactccat cttttgaggt ggacgatgag ggcaatcctt
    40621 tctatgtagc aacggtttac caaaagcaat ttggacttgc tgttcctcgt cctgcttcag
    40681 tcattatctt ggatgctaca aatggagaaa ccaaggaata cagcttatca gatgttccag
    40741 aatgggtgga caggatctat ccagcagagg aaaccattga gcaaattaac tacaacggca
    40801 agtacaagga cggtttcttg aatgccatga tttccaagaa aaacgtgacc cagactacca
    40861 agggctataa ttacttgtct atcggtaatg acatctatct ctacacaggt gtgacgtcgg
    40921 ctaatgcgga tgagagtaat cttggtttca tccttgaaaa tatgcgaaca ggagaaatca
    40981 ctaagtatag cttggcttct gcgacagaag aatcagcccg tgaatcagca gaaggtgctg
    41041 ttcaggagaa atcctacaaa gcaaccttcc caatcctcat caacctcaat gacaagcctc
    41101 tctacatcat gggcttaaag gacaatgctg gcttggtcaa agagtacgcc ctggtagacg
    41161 cagtcgagta ccaaaatgtt atcgttgcta ctacagtgga agagatgctc agcaagtatg
    41221 ccaataaaaa cgaccttgaa attgacaatg caacgacaga aagcatcaag ggagtagtag
    41281 cagacctcaa atcagctgtt atcaagggcg acaccgtcta cttctttaaa gttgatggca
    41341 agatttacaa ggttaaggcc tcagtatccg atgaccttcc ttaccttgaa aatggtagaa
    41401 ccttcgaagg tcaagtagga aaagacagct atctcaagac ctttaaagta cagtaatact
    41461 ctgcgaaaat ctcttcaaac cacgtcaact ttatctgcaa cctcaaagct gtgctttgag
    41521 caacctgcgg ctagtttcct agtttgctct ttgattttca ttgagtataa aaaaggttta
    41581 tttaagaaat atatgttata gtaaaataaa ttaagactaa tggaggacaa agaaatgggt
    41641 ttttatttga tggttgcttc aatgctactc ggactgctcg ctttgaagat aggtttttct
    41701 cagttcaagg agaacaaaga taaattctta tctatcttaa caagtttagc tgggctagcc
    41761 cttgttctcg tggctgtctg gttaggatgg cccaaataaa tagaaaaacc tcggtcaaac
    41821 cgaggttttt cagatgaatt tcttggttgt ggctaaaaaa tatgctacac tatcaatatg
    41881 aagattttaa tccctacagc aaaagaaatg aatacagact tgccgagtat cgagacaact
    41941 cttttaagat cagaaagtca ggctgtactt gatgccttgg ctctctattc tgccagtcaa
    42001 ttggaaagtt tctacaaggt atcagctgag aaggctgcgg aagaatgtca aaatatccaa
    42061 gctttgaaaa ggcaaactgc tcaacactac ccagccttga agctttttga tgggcttatg
    42121 taccgtcata tcaagagaga caaactgacc gaggtagaac aagcctatct tgaaaatcat
    42181 gtcttcatta cctcggcttt gtacggagtc gttccagcct tgtcgcctat ggcacctcac
    42241 cgtttggatt ttttgatgaa attaaaagtc gctggtaaga ctttgaagag ccattggaag
    42301 gcagcctatg atgaagctgt gaagcaggaa gaagtgattt tctctctctt gtcatcagag
    42361 tttgagactg tattttctaa ggaaatcaga gcaaagatgg tgaccttcaa attcatggag
    42421 gatagaggcg gtcagctgaa gattcactca actatctcca agaaagcgcg cggggccttt
    42481 ctaacagctt taatagaaaa tcaagtacaa actgtggggg aagcacgtcg cttgaacttt
    42541 gctggatttg tttaccgaga agatttgtca caaccacagg gattggtttt tgtaaaggaa
    42601 gtatagaaat cagataagag aaaagtcagc acgattatgc tgactttttt gataacttat
    42661 aaagtattgg acaaatgaat ctcatttgat aaggtgttaa gatgattaac caaattcatg
    42721 attgacagat gatttcggca aataaacttc ttggtaatcc ctgaaatata gaaattagac
    42781 acgatgatat cgtaatctgt ctgatttaaa atctcaggac ttgtttttaa ttcatcccaa
    42841 atatcgaagg taaagcggtt attacagtaa taggaaagca tatcaacaaa ggtaagggat
    42901 atagcatggt caaagttact gataatcaaa accttgatag gtggtcgatt ttctaacaac
    42961 tgagtagaga ggttttcagc atgggttaag atggtgtaca tcaagtgttc caactgttca
    43021 ggatggtcat gctgtccaat tgcctgacgg tattgagcca gttcttgacg cgcacttccc
    43081 ataaagtctg ggaagtaaac ttcaaatttt ttaatcgtca atgctttttg ttcaaataag
    43141 attggggtag aaaagatttc ctgtctttca aagaaagcag tgttgtgtaa atgccaaatc
    43201 agctcatcgt ggttagtaaa agtaatctta tattgttttg ataggcgttc taatatttga
    43261 ctaagtaatt ggtaagagta tcttgcttga ctgttatctt ctaaagagtt gaagaattct
    43321 tgaggatcta ggaaaatatc attctgaaga aaagaaataa aaatttgagc gattgtatct
    43381 ggagtcattt caagcccaaa atgtttagag aaataagcaa gtgtctcctg gaaatttgga
    43441 atgctcttta aaagaggata atattctttg taaaagtggt tggggaggtc aatgaagtga
    43501 ccatttttaa ccctgtggat actgatagct atcatcagtt tgtacattct ataaattgaa
    43561 aatcgcattg gataattcgt gattttatag aagaagtcag caaactctgt taaatcttcc
    43621 tcaggaaggt caggaaaagg ccaatctagg aaataatagc gctcagaaaa gtattgggca
    43681 aaaaagtaac gaatatcgat ctcatttcca cgtatctcgg atggagtaaa agataattca
    43741 atttgaaact gagatgaaag aacctttgta atatttctgc ccagtcgata gaggttggca
    43801 ctactaaagt aaagattttc tattgttcga taaataggta gcccctcatt gaagaacata
    43861 tactctagta attgaaaaga ttgagaatta gcaagaaaat attgataaat atcttctaca
    43921 ctagtgttaa cttctaaatc tatcatgatt ccgttaacag aagattggat gttaatagat
    43981 ggaaaggcaa tacgtaattc atttaaatca ctttttaaga ttcgttctgt acatcctaat
    44041 ttttcggcta gttcatgtaa tttcatccaa ttacgattct gaattagggt ttccattaag
    44101 cgtaattgtc tttgaacttt tgtggataaa aggtttctca tacgggtatt tcctccataa
    44161 ttcattatat ctttttttgt ctaagtagta taaaaaaatt agagcaagaa aattgtcgtt
    44221 agtaatcgga aaaattagaa taatataagt ggcttttaag tgcaaaaaca gaatatatta
    44281 attgttcaga aaatattgat taatgcctca aatagttgta cattgtaaac tataaaatac
    44341 tatctaggag tttaaaatga aacattcaca taaaaaatca tttgactggt atagcatgca
    44401 acaacgttat tctattcgta agtattactt tggtgcagtt agtgtcttgc tcggtaccgc
    44461 tttggtatta ggtgcagcag ctagtgtcca aacagtacaa gcggaagaaa acaaacaaga
    44521 aactaccaat agtatttctg ttggtagggg agaagcagct actaaaccag cagaggtttc
    44581 tgcgtctaat aaagagaaaa cctatgcagc tccaactgta gctaatccag tagaaacgac
    44641 tccagttaaa actggagaag ttactaaacc agcagaaaaa gttgaagaag caaaagacaa
    44701 aaaagaggaa gtaacgcatc aagatgctat tgacaagtca aaattgttaa cggctctttc
    44761 gcgtgctaaa aagttagaaa gcaagttata tacagaagca agtgctgcta acttgcaaac
    44821 aagtatccaa gctggtcaaa gcttgcttgg gaaagcagat gcatctgaag ctgaattatc
    44881 agcagcagag tcatctattc aatcatctat tattggtcta gaacttcgtt ctaactctaa
    44941 caaaggaact gtatcagaaa cgcctgtagc aaagaaagct aatatagttg aagcgaagga
    45001 agagactaaa ccagctgtaa caacgactga acgttcagct gttgatagcg ctattttgcc
    45061 aattagcaca gctgctaaag tagaaacaac ttcagctcca gcatctacta atgaaatctt
    45121 gaaaccaagt ttgagccttt ctgatgctcg ccaaaatcca gctatccgta aggaagatgt
    45181 tgatagaggg tatagtggtt ttagaacagc aggaagtggc tttagagcgg ctggaagtgg
    45241 ttttagagca gctggaccag aaaataaacc aatcttaaat ccaaataata ctattgcatt
    45301 ttctgacatc agtcagggct tacattcatt tcgtggtata ggacattcac gtggtggtcg
    45361 tgaaatccat tatgatgtaa cgacagtacg tagaggtaat agattgaatt tcacaattaa
    45421 atactctggt ccaggtgaat ttgttaataa taattttatc ttggataaag gggatggatt
    45481 tggaaatcct tcaaatgcaa cgatcacaag ttcaaatcca agagtaaggg agcaatcaaa
    45541 atctattagt cagggtgcca actacgtatc tcactcagga tacagtatga cctctgctac
    45601 ttcaacaaat acggaacaaa ctatcagatt tagtttgcct atcattaatc caaatggtga
    45661 tttgtctgtt cgtttgaaac cagttacttt taacgtggac caaggtggtg gtggtgccgc
    45721 tactagcaat gacccataca gtaactccaa ctattatcat agagcaaatc cactcctctt
    45781 ggatgcaaac ccttatggtg gtactaataa taagactgtt tcagaagaca ttgacttcca
    45841 aactgtctat cttccaacta gtaagttacc agaaggtcaa actagattag ttcgagaagg
    45901 tgaaaaagga caacgtcaaa ttacctataa agtacatcga tttggtaacg aaacactttt
    45961 aggattgcca attagtaata gagttactaa agaagctaag ccacgtatta tgcaaattgg
    46021 tgtggctaaa gagttaatcg atacagtaaa accacgtgtt gatcaaaata aagttggtga
    46081 tacaaataac ctcactttct atcttgataa cgatggaaac ggtgtttata ctgagggcgt
    46141 agacgaactt gttcaaaaaa ttgctattaa agatggagcc aagggtgaaa aaggggacca
    46201 aggtgaacgc ggtctaaccg gagcgcaagg tactaaaggt gaaaagggag atcagggtga
    46261 acgcggtcta accggagccc aaggagctaa aggtgaaaaa ggtgaacaag gtcttcaagg
    46321 gcgtgatggt gaacaaggac caaaaggaga agatgggaaa accccaacag ttaaagtaac
    46381 tgatggtcaa gatggaacgc atacaattac aattaacgat ggtaaaggtg gtataactac
    46441 tacagtagta agagatggat ttgatggtgc aagccctctt gtttctactc atagaaatga
    46501 agcagataaa acaacaactg ttattttcta ttatgatcta aatgataata atcaatttga
    46561 tgaaggagat acaaaactta aagaagttgt tatcgcagat ggaaaacaag gaccaaaagg
    46621 tgacaaaggt gataacggaa aagatggatt cacaccagaa gtaacagtta cagataacaa
    46681 taacggaaca cacaccatca caatcacaca accagacaac agaccatcat taacaacaat
    46741 cgttaaaaac ggtgaagatg gaaaaacacc aaaagtcaaa gcagaacgag atgatgcgaa
    46801 gaaacaaaca acattaacat tctacattga caaagatgga gatggaagtt acacagcagg
    46861 aaaagacgag ttagttcaaa caacagtagt taaagacgga caagacggag ctgcaggagc
    46921 atctggacgt gatggaaaag aagtattaaa cggaaaagca gacccaacaa cagaaggtaa
    46981 agatggagac acattcgtaa atacacaaac aggagatgta ttcgttaaga aaggtaacac
    47041 ttgggaacca gcaggaaaca tcaaaggacc gaaaggtgac aaaggtgcag atggtgctaa
    47101 aggcgaaaaa ggagaccgag gcgaacgcgg tctaaccgga gcgcaaggta ctaaaggtga
    47161 aaagggagat cagggtgaac gcggtctaac cggagcgcaa ggtactaaag gtgaaaaagg
    47221 agaccgaggc gaacgcggtc taactggagc gcaaggtcag gcaggtcgtg acgctgtaac
    47281 tccaaccgta accgttaaag ataataaaaa tgacggcact catactatca ctattaacga
    47341 cggtagaggt aatgttgcaa gtactgttgt aagagatggt ttcgatggcg caagtccatt
    47401 agttgcgact caacgaaatg aagcagataa gacaacaact gttatcttct attatgatca
    47461 aaatggaaat aatgaattag atgcttctga taagaaatta aaagaagtta ttattgcaga
    47521 tggtgccaaa ggtgaacaag gtcttcaagg tcgtgatggt caagacggag ctcagggaca
    47581 agcaggacgc gacggtaaag acgtgttgaa cggcaaagct aatccagaag taaatcaagg
    47641 taaagacggt gataaatacg tcaatacaga aacaggcgac gtcttcgtta agaataacgg
    47701 caactgggat aaagagggca acatcaaggg acctaaaggg gacaaaggtg aacgcggaga
    47761 agatggtaag actccagaag taactgtaac tccaggtaaa gatggccata gtactgacat
    47821 tacattcact gttccaggta aagatccagt aaccttcact gtaaaagatg gtaagaatgg
    47881 taaagatggc cgtgcaccga aaatcaaagt agaagatatt acttcacctt caagaattag
    47941 acgcgataca gatgctgctg caactccaac gcgtaacggt atccgtgtta cagtttatga
    48001 tgatgttaat gacaatgggg tatacgacga aggtgtcgat aaagtattaa atagtaaaga
    48061 tatttataac ggtatagatg gacgtgatgg ttcagctcca actattacta caaaagataa
    48121 tggagatgga actcacacta tcacagttca aaacccagat agttctgaat caacaacagt
    48181 tgttaaagat ggtaaagacg gtaaaactgc gaatatcact acaacagaaa acccagatgg
    48241 aagccacaca attacggtga caaatccaga tggttcaact aaagaaactg ttgttaaaaa
    48301 cggtaaagac ggtaagactc ctaaagttga agtaacggat aacaacgatg gaactcatac
    48361 tgttaaagtt acagatggag acggcaatgt taccaacgct atcatcaaag atggtaaaga
    48421 cggtaaagct gcaacagcaa caactactga aaatccagat ggaagccaca cagtaacaat
    48481 cactaaccca gacggaacta agaatgagtt tgttgttaag aatggacgtg acggtgttga
    48541 cggacgtact ccaaccgcat ctgttcgtga taatggagac ggaagtcata caatcgttat
    48601 tacaaatcca gaaggtgtga caactgaaac cacagttcgt gatggtaaat caccaaaagt
    48661 gactataact gatgaacaaa atggaactca taagatctct gttctaaatg gtgacggaac
    48721 aactactgaa acaatcatta aagatggtaa atcaccagta gcaacagtta gagataacca
    48781 agatggtact tacactattc gtgtggaaaa cggtaatggt actgtttctg aaaccacagt
    48841 tcgtgacggt aaatcaccaa ctgctaaggt tgtggataat ggagatggaa ctcacactat
    48901 cacagttgtg aactcagacg gaacaactac aacaactaca gttcgtgatg gtagagaacc
    48961 aaaacttgaa gttattgata acaacgatgg ttcacacact attaaagtga caggtgctga
    49021 tggtaaagga acgacaacta caatctttga tggtaaatca ccaaaagcga acatcgttga
    49081 taacggagat ggaactcata cattaacaat cgtagattct gatggtcgtg aatacaaatc
    49141 tattatcaaa gatggtaaag acggcaaaga tagcgtttca ccaactgtaa ctgttaaaaa
    49201 taataacgat ggaactcacg ttgttacaat cactaatcca gatggaagta agacagaaat
    49261 ggtgattaaa gacggtaaag atggaaaatg tggatgccaa gacaaaccag taacaccatc
    49321 aaatgacaaa ccagttcctc caacaccaaa tgtgccgaca ccagaagtac cggttaaacc
    49381 tgtgccagcg caaccaacac caaatgtacc gacaccagaa gtgccagtac aaccaactcc
    49441 agctgttcca acaccagaag taccggttaa accagtacca gcggttccag aacaaccagt
    49501 agtaccaaca ccggctcaac cagcaactcc agtaaatgct aatccagtag caccaactac
    49561 aggtaaagaa aaccgtgggg acaaattacc tgaaactgga agccaatctg attatatctc
    49621 tgttctttta ggtagcggta ttctattgag cctatatgta ggacgaagaa aagaagatta
    49681 atcagccaaa agcagtcact aatttctcga aagaagatga taagtgatag atctcgaatg
    49741 aaagaatatt cttagaatga ttttcctatg atggaaaaga ctgctaaaaa ccttgagtgg
    49801 taacgctcaa ggtttttttg gatggctgat gtttgtctga aaatcttgat tgagaaataa
    49861 aaagccaccc tattaggtgg ctataattga tttagacttt ctaaaatttt ctatttttta
    49921 ctgaatcgct tcgaccaagt cttgagcttg tttttcaagt gagtttagga ctgtttcttc
    49981 aagaaccaat tttccgtctg cccaggcaga gtcattaaca cgtgcagcag tgaaatcacc
    50041 aacgccttgt gtacggataa atggcaagag gtctttgtag atagcgaaaa gttgatcgtg
    50101 ccctgcattg gctacagatg atactgtgac aaacttgtct tgaagggcag aaacgccacg
    50161 tgtatcagac aagtcaaggg cacgagatag ccagtcaagc aagtttttca ctgtaccagg
    50221 gatagagaag ttgtagactg gagagaaaat ccagatagca tccgcaacga gaactgcttc
    50281 acgagcagca gctacagctg gatgtgttgg aacttccaaa tcttggctga agagaggaag
    50341 ggctgaataa tcaaggtagc taacttccgc tttaccagca agtgctttct cagcttcgag
    50401 cgccatttgg tggttgaaag aaccttgacg tagtgatccg acgataaata atactttttt
    50461 agacatgatg tgtctccttt ttcttaaaat ttcgagaact ctctcgttgt tataaaatat
    50521 attacaacaa aacaaaagaa cttgcaagaa atttgctcag agtttatttg aaaattgtaa
    50581 aatttcggaa actctaatca tttgtgactg aaaaagtaag aactgtttca ctttcaaaat
    50641 cggttttgtt tgaagggaga aatagaattg gttgctcaaa gcacagcttt gaggttgcag
    50701 ataaagctga cgtggtttga agagattttc gaagagtatt cttatattca cttgtaataa
    50761 agcttaatag atggtaaaat agacgagtga aaaaaagaca aaacaaagca aaaaataatc
    50821 tactgtggca gtatggtcta gggatgacga ttttgtttgt ggttatcagt ggttcctttc
    50881 tgtatatggt ttctcttagc atgaaaccct atcaaacagc taaaagtgaa ggagaaaaat
    50941 tagctcagca gtatgcagga ttagagcagg tcgatcaggt tgatttatac aatggcttgg
    51001 aatcttatta cagcgttctt ggtcgtaata aacagcaaga agcgcttgct gtcctgattg
    51061 gtaaagatga ccataagatt tacgtttatc agctaaatca gggtgtttca caagaaaaag
    51121 cagaaacggt ttctaaggaa aagggagctg gcgagattga caagataacc tttggccgtt
    51181 atcaagataa gccaatctgg gaagttaagt caggatctga tttttatcta gtagattttg
    51241 aaacaggagc attggtcaac aaggagggcc tatgaaacta tccaaccgtg ttttagaaat
    51301 ggaagaaagt gtgactttgg ctgctggagc ccgtgccaaa gcactgaagg ctgagggtag
    51361 agatattttg tctctaacct tgggtgagcc agatttcact actcccaaaa atatccaaga
    51421 tgccgccatt gcatcgattc gagatgggcg tgcttctttt tatacagtaa cctcaggtct
    51481 gccagagctt aaggcggcgg tcaatagcta ctttgagcgc ttttacggct attctgtagc
    51541 gtcaaatcaa gtgacagtcg ctgcgggagc caaatattct ctctatacct tctttatggc
    51601 tgtggtcaat ccaggtgatg aagtcatcat cccaacccca tactgggtta gctatggaga
    51661 tcaggtcaag atggcagaag gcgttcccgt ctttgtttct actaaggaag acaatcactt
    51721 taaggtgacc gtagatcagt tagaagcagc tcgcactgac aagaccaagg ttttggtgct
    51781 gaattcgcca tctaatccca caggtatgat ttacacccgt gaggaactct tggcaattgg
    51841 aaactgggct gtagaaaatg atattctcat cctagcagat gatatctatg gccgcttggt
    51901 ttataatggt catgagttca caccgatttc tagcctatcg gaagcgattc gcaagcaaac
    51961 agtggtcatc aatggtgtgt ctaaaaccta tgccatgact ggttggcgga ttggttatgc
    52021 cgttggagaa gcagatatta tcgctgccat gtccaagatt gcaggtcaaa caacttcgaa
    52081 tccgtcagca gtagcccaat atgcagcagt tgaggctcta tcaggtgagc aagatactgt
    52141 agaaagcatg cgtcaggcct ttgaggaacg tcttaatacc atctatcccc tccttgcaga
    52201 ggtaccagga tttgaagtgg tcaaaccgca aggggccttc tatctcttcc caaatgtcaa
    52261 aaaggccatg gagatgaaag gctacacgga tgtgacagac tttacaactg ctatcttaga
    52321 agaagccgaa gtggccttgg taacaggagc tggatttgga gcaccagaaa atgtccgcct
    52381 cagctatgcg acagacctag acacgcttaa agaagcagtc gaacgcttga aagcatttat
    52441 gggtagtgag aatgattgat cattttgaga ttaaggtaaa ggatttacaa atttcagaag
    52501 gattttatag gagttttctc gctcctttgg actataaatt ggcttttaag actagttctc
    52561 taattagttt tctatccccg aacagccctc atcctggtgg tgatttttgg ctgactcagg
    52621 gaacacaaga tcctgttcat tttgcttttt tagcagaaaa taaggaagaa gttcaggctt
    52681 gttatgaggc tggcttagag gcaggtgggc gagacaatgg ggctcctggt tatcgaagtg
    52741 agcatccgat ttactatgct gcttttatga ttgacctgga tgggaacaat atagaggtgg
    52801 tttgccataa agaataaaaa agaaaaggaa aaataatgac aaaacgtgta acgattattg
    52861 acgtaaaaga ctatgttggt caggaagtga cgattggcgc ttgggttgcc aacaaatcag
    52921 gaaaaggaaa aatcgctttc ttacaattgc gtgatggaac agccttcttt caaggtgtgg
    52981 cttttaaacc aaactttgtc gaaaaatttg gtgaagaagt gggacttgag aagtttgatg
    53041 ttatcaaacg cttgagccaa gaaacgtctg tttatgtgac aggtattgtc aaagaggacg
    53101 aacgttctaa atttggctat gagttggaca tcacagacat cgaagtgatc ggtgaatctc
    53161 aagactaccc aatcacacca aaagaacacg gaacagactt tttgatggat aaccgtcact
    53221 tgtggctacg ctctcgtaag caagtagctg tgttgcaaat ccgtaacgct attatctatg
    53281 caacttatga gttctttgac aagaacggtt ttatgaagtt tgacagccca attctttcag
    53341 gaaatgcggc agaagattct acagaactct ttgaaactga ctacttcgga acgccagcct
    53401 acttgagcca atcaggtcag ctttacctag aagcaggggc tatggctctt ggtcgtgtct
    53461 ttgactttgg tccagttttc cgtgctgaaa aatcaaaaac acgccgtcac ttgactgagt
    53521 tctggatgat ggatgctgag tactcatact tgacacatga tgagtcgctt gacttgcaag
    53581 aagcttatgt gaaagctctt ctacaaggtg ttcttgaccg cgcgcctcaa gccttggaaa
    53641 ccttggaacg tgatacagaa ctcttgaaac gctacattgc agagccattc aaacgtatca
    53701 cttacgatca agccattgac ctcttgcaag agcatgaaaa tgatgaagat gctgactacg
    53761 agcatcttga gcatggtgat gactttgggt caccacacga aacttggatt tcaaaccact
    53821 ttggtgtgcc aacatttgtc atgaactatc cagcagccat caaggccttc tacatgaaac
    53881 cagttcctgg aaatccagag cgcgtgcttt gtgcagactt gcttgctcca gaaggctatg
    53941 gagaaattat cggtgggtct atgcgtgagg aagattacga tgcccttgtc gctaagatgg
    54001 atgaacttgg catggatcgt acagaatatg aattctacct tgaccttcgt aaatacggta
    54061 cagttccaca cggaggattt ggtatcggta tcgaacgtat ggtaaccttc gcagcaggaa
    54121 caaaacatat ccgtgaagct attccattcc cacgtatgtt gcaccgtatc aaaccataag
    54181 aacaggaaaa cgcccatgtg gcgttttttc gaattctagt gtttttactt gcgtaaaaaa
    54241 attttttcta gtataatagt ttattgtgag cgaacctcac ttaccccttg caaagtcttg
    54301 gggtcattag accaaaagga ggaacatatc aatggctaaa tacgaaattc tttatatcat
    54361 tcgtccaaac attgaagaag aagctaaaaa cgctttggta gcacgttttg actctatttt
    54421 gactgacaac ggtgcaactg ttgttgaatc aaaaacttgg gaaaaacgtc gtcttgcata
    54481 cgaaatccaa gatttccgtg aaggacttta ccacatcgtt aacgttgaag caaatgacga
    54541 tgcagctctt aaagagtttg accgtctttc aaaaatcaac gctgacattc ttcgtcacat
    54601 gatcgtcaaa attgacgcgt aagaaggtaa actatgatta acaatgttgt acttgtaggg
    54661 cgtatgacac gtgacgctga gttgcgttat accccatcaa atgtagcagt tgcgactttt
    54721 actcttgcag taaaccgtac atttaagagt caaaatggtg aacgtgaggc tgattttatc
    54781 aatgtcgtta tgtggcgcca acaggctgaa aatcttgcta actgggctaa aaaaggctca
    54841 cttatcgggg tgacaggtcg tatccagact cgtagttacg ataaccagca aggacaacgt
    54901 gtctacgtga cagaggtcgt ggctgagaat ttccaaatgt tggaaagccg tagtgtgcgt
    54961 gagggccaca caggtggagc ttactctgca ccaactgcaa actattcagc acctacaaat
    55021 tcagtaccag acttttcacg taatgaaaat ccatttggag caacaaaccc attggatatt
    55081 tcagatgatg atttaccatt ctaatggaca actaaactta taaaggagaa aaaacatggc
    55141 tcaacaacgt cgtggcggat tcaaacgccg taaaaaagtt gattacatcg cagcaaacaa
    55201 aattgaatat gttgattaca aagatactga gcttcttagc cgtttcgttt cagaacgtgg
    55261 gaaaatcctt cctcgtcgtg taacaggaac ttcagctaaa aaccaacgta aagtaacaac
    55321 agctatcaaa cgcgctcgcg taatggcttt gatgcctttc gtaaacgaag attaaagaaa
    55381 agacccggtt gggtctcttt ttcatgagct tgaaaacaag aaagcgaatt aagaccccaa
    55441 cgggtctttt ttcacaagct ttgaaatgag agagcgagtt aggctcagtg gctccctttt
    55501 tacgagtttg aaaacaagag aacacattaa aagcacgatg gagctttttc ataaatatgt
    55561 cttttttctc atccaagaaa ttaaaataaa aatgataaac tttttgaaaa taggggattt
    55621 gcctacttta tgatataatg gaaagagtta gattgaaaga ggtagtgaga tggacgcgaa
    55681 attaagatac aaggcaaaga agatcaagat tgtctttttt gatattgatg atacattgcg
    55741 gaattcaaag acagggttta ttccaactac aattcccact gtttttaaac agttgcgtga
    55801 aaaaggaatt ttaacaggaa tcgcctctgg acgtggcatt tttggtgttg ttccagagat
    55861 tcgtgatctc aagcctgact tttttgtaac tttgaatgga gcttatatcg aagataaaaa
    55921 aggtcaggtc atttatcagc atcagattga aaagtcagat gttgaggagt atatctcttg
    55981 ggctaagcaa gaaggaattg agtatggctt ggttgggagt catgatgcca agttgtcgac
    56041 tcgcaccgat atgatgagtg aagctatcaa tccaatttat cccgacttag atgtagatcc
    56101 cgatttccat gaaaaagaag atatctatca gatgtggact tttgaagata agggagatga
    56161 cttgcacttg cctgacagtc tctcagacaa acttcgcatg gttcgttggc atcaacattc
    56221 gtctgatatt gtgccgattt caggctccaa agcgacgggg gtggaaaagg ttgtggaaca
    56281 ccttggcttg aaaccagaga aggtcatggt ttttggagat ggtctcaacg acttggaact
    56341 ctttgattat gctggaatca gcgttgccat gggaatttct catgataaaa tcaaagaaaa
    56401 agcagattat attacaaaaa cattagaaga agatggcatt tttgatgcct tagaagtatt
    56461 tggtatggta gaaaaagaat tgcattttcc acaagtagac attgaaacag tagaaggtcc
    56521 tcttgcgact attaagacca atcacggaga cttacgtatc aagcttttcc ctgaacatgc
    56581 tcctaaaaca gtggctaact ttgtatctct ttcaaaagat ggctactatg atggtgtcat
    56641 tttccaccgt attatcaagg actttatgat ccaaggtgga gacccaactg gaactggtat
    56701 gggtggcgag tcaatctacg gcgaatcttt tgaggatgaa ttctcagaag agctttacaa
    56761 tatccgtggt gctctttcca tggcaaatgc tggtccaaat accaacggca gccagttctt
    56821 tatcgtgcaa aaccaacacc taccttattc gaagaaagaa attactcgtg gtggttggcc
    56881 agaaccgatt gcagaaatct atgccaatca aggtgggaca cctcacctag accgccgtca
    56941 cacggttttt ggtcagttag ctgatgaagc atcttacgct gtcttggatg ccattgctgc
    57001 tgttgagaca ggagctatgg acaagccagt tgaagatgtt gtaattgaaa ctattgaaat
    57061 cgaggactaa gatgaaaatc ggtgataagc taaaggggcg tattacaggg attcagccct
    57121 acggtgcctt tgttgagtta gagacgggtg atacggggct gattcacatt tcagaaattc
    57181 ggacaggatt tattgaaaat attcatgaaa ccttgaaagt cgatgaagag gttcaagttc
    57241 aggtagtgga tttagatgaa tttacgggga aagccagtct ttctatccgc actttggagg
    57301 aagaaaagta ccagtttcct agacggagac gcttttcaag cgaccgcttt aactacggtt
    57361 ttgctccctt tcgacgaatg ctaccaatct ggactggaga agccctgcac catttaaaaa
    57421 agaagaagta gtttagagtt ttatattttt tgtaatgtta atatgaattt gctataatag
    57481 gaccatggaa gaagaacaat tattaaaatc aggagagcgc attaaccagc tcttttcgac
    57541 agatatcaaa atcattcaaa atagagaggt ttttagctat tcggtggata gtgttctctt
    57601 atcacgattt ccacgttttc ctaagaaggg gttgattgtg gatttctgtg ctgggaatgg
    57661 agcagtgggg ctttttgcta gcactcgtac tcaagcacag atattgtctg ttgagattca
    57721 ggagcgtttg gcggatatgg ctgaacgctc tgtccgtttg aatggattag aggagcaaat
    57781 gcaggtcatc tgcgatgatt tgaaaaatat gcctgctcac attcagggga gtaaggtgga
    57841 tatgattttg tgtaatccac cctatttcaa ggtgaatcct tattctaatc tgaacgagag
    57901 tgaacattat ctcttggctc gacacgaaat cacgactaat ttggaagaaa tctgtcgtag
    57961 tgcccagagt attctcaagt ctaatgggcg tttggccatg gttcatcgtc ctgatcgact
    58021 tctggatatt ttggatacgt taaaacgaca taatctagcc cctaagcgcc tgcagtttgt
    58081 ttatccaaaa agagaaaagg aagccaatat gcttttgatt gaggcgatca aggatggctc
    58141 aacaagtggc tttaaggtct tacctccttt gattgtccac aatgatgatg gctcttatac
    58201 gcccgaaatc gaagagattt attatggatc ataaggctta tatgtacgtt ctggagtgtc
    58261 gtgatggatc ctactatata ggctatacga ctgatatgag aagacgcctc gctatccaca
    58321 atagtgggaa gggagccaaa tatacacgag cacgcttgcc agtcaaactt atctatgctc
    58381 aaggttttgc cagtaaggaa gaagccatgt cggctgaagc tcttctcaag cgtaagaaga
    58441 ggccacagaa ggaagaattt ttatctgaaa atcaagatag aaatttactc agttattttg
    58501 aagaatcgtg gggagtcctt tgactcctct tacctttgtc aaaaagcgaa aaaaatgcta
    58561 caataaagaa aataaacaaa atgaggtatt atcatgtcca agattctagt atttggtcac
    58621 caaaatccag actcagatgc catcggatca tctgtagctt ttgcctacct tgcaaaagaa
    58681 gcttacggtt tggatacgga agctgttgcc cttggaactc caaatgaaga aacagccttt
    58741 gtcttgaact attttggtgt ggaagcacca cgtgttatca cttctgccaa agcagagggg
    58801 gcagagcaag ttatcttgac tgaccacaat gaattccaac aatctgtatc agatatcgct
    58861 gaagtagaag tttacggtgt tgtagaccac caccgtgtgg ctaactttga aactgcaagc
    58921 ccactttaca tgcgtttgga gccagttgga tcagcgtctt caatcgttta ccgtatgttc
    58981 aaagaacatg gtgtagctgt gcctaaagag attgcaggtt tgatgctttc aggtttgatt
    59041 tcagataccc ttcttttgaa atcaccaaca acacacccaa cagataaaat cattgctcct
    59101 gaattggctg aattggctgg tgtgaacttg gaagaatatg gtttggcaat gttgaaagct
    59161 ggtaccaact tggctagcaa atctgctgaa gaattgattg atatcgatgc taagactttt
    59221 gaactcaacg gaaataatgt ccgtgttgcc caagtgaaca cagttgacat cgctgaagtt
    59281 ttggaacgcc aagcagaaat tgaagctgca atgcaagctg ccaacgaatc aaacggctac
    59341 tctgactttg tcttgatgat tacagatatc gtcaactcaa actcagaaat cttggctctt
    59401 ggtgccaata tggacaaggt cgaagcggct ttcaacttca aacttgaaaa caatcatgcc
    59461 ttccttgctg gtgccgtttc acgtaagaaa caagtggtac ctcaattgac tgaaagcttt
    59521 aatgcgtaag attttgggtg tcagctcaaa atcggaaagt ctagtttgcc ttatatcgaa
    59581 aggagtttcg gctccttttt tctaggagtg aagtatgtta gaaaatggcg atttgatttt
    59641 tgtgagagat gggtcagaca tgggacaggc catccagact tccacaggta actatagcca
    59701 tgttgccatt tatttggatg ggatgattta tcatgctagt ggacaggctg gtgttgtctg
    59761 tcaagaaccg gcagacttct ttgagtccaa tcatttatac gacctctatg tttacccaga
    59821 aatggatatc cagtcggtga aggaaagagc ttgcaaacat cttggagcac cctacaatgc
    59881 ttctttctat ccagatgcac ctggtttcta ctgctcccag tatatagcag aaatcctacc
    59941 tatttttgaa actattccta tgaaatttgg agatggggag caggagatta gtgatttttg
    60001 gagggagtat tacatagaac taggtctgcc tgttcctctg aaccaagctg gtaccaatcc
    60061 tagtcagttg gcagcatcgc ctctgttaca atgtaaagaa aggaatcttc atgattcaga
    60121 tttttaatcc atctcgtttg acgagacagc catttttgga gaattgatcc gctatctgga
    60181 ccagtatgag gatgtgattc tacgggaaat taaggctcaa tttccagatg ttgcagttga
    60241 taaactcatg gaagagtata taaaggcagg cttgattcta cgtgaaaata agcgctatta
    60301 cctcaatttt cctacgcttg aatcacttga tagtcttgaa ctggatcaag agatttttgt
    60361 cagagaagct agtccggtct atcaagcctt gttggagcag agttttgaga cggaattgcg
    60421 caatcaaatc aatgcagcta ttttagttga aaagacggac tttgcgcgca ttaaaatgac
    60481 tctgtccaat tatttttaca aggtcaaaca gcagtatcct ttgacagaaa aacagcagga
    60541 gctctatgac attttaggag atgttaatcc tgagtatgca ctcaagtata tgacggcttt
    60601 tttgttgaaa tttctcaaaa aagaccagct tatgcagaaa tgccgtgata tctttgtgga
    60661 cagtttggtt gtcttaggct atattgtgca aaatgaagat ggaaagtatg agttggctat
    60721 cgattttgat aaggagagat taactttcta cttagcgtga tttcttgttt ctgagtacat
    60781 tgtttgactt tccttagtat tcggtataaa ctatatgtaa ccggtaacac atatcggaat
    60841 aaactaaagg agacaatcat atgtcacttg aaaacaaatt ggaacaagca acaggcgctg
    60901 tcaaagaagg ttttggtaaa gttactggag acagcaagac agaacttgaa ggagctgttg
    60961 aaaaaacagt tgctaaggca aaagacgttg tagaagacgc aaaaggtgct gtagaaggtg
    61021 ccgttgaagg tttgaaaaac gtttttacta aagaatagga aaaaatcaag ggtttcattt
    61081 tcccttgatt ttttctattc ttataaataa ttttctgcga cggctgtatc tcctgggtag
    61141 gattctttct tgccctggat gatttggtaa caatcggctc ccttacccgc aataataact
    61201 gcatctaatt cgtgatttgt gatagccatt gccgccttga tggcttcttg gcgatccgca
    61261 atcttctcaa caggatgatt gatgtagcta ctaatttcat ctgcaatggc cattgggtct
    61321 tcatagttag ggtcatcagc agtcagaaag acttgaatct cagggtgttg attgaggagg
    61381 aggccaaagt ccttacgacg actttctccc ttgtttcctg ttgatcccag aaccagagca
    61441 atctttccgg tttgatgagt ttcaaccaca ttgatgagtt ttttcagact atccccattg
    61501 tgggcatagt cgatgaagac cttggctcca tttttctgag tgaggacttc catacgacca
    61561 ggaacgcggg ttgcagcgat gccttttttg atgtcctcaa gacttgctcc gagacggaga
    61621 caagcaagtc cagcagcaac tgcattttct tggttgaagt tgccaatgag ttggatatca
    61681 taatctccag cgagtttacc cgtagctgaa aagctaaagg ctttggaatt ctcgatttgg
    61741 ttatcaaatt ggctaccata gaaatcatgg tcttgatctt taacctgttc tttcaagact
    61801 gagaagtggt ccatgtcact gttaatgatg actgctcggc tattttccat caagagacgc
    61861 ttgtggtaga aatagtcttc aaagctaggg tgttcaatcg ggccgatatg gtctgggctg
    61921 atattgagga aaactcccac atcaaaggtt agaccataga cacgtctgac cagataggat
    61981 tgactggaga cttccatgat gaggtgggta cggtcatttt gcacagcctg attcatcatg
    62041 tcaaagaggt caatactctc aggggttgtc aacgctgact taaagaaagt ctcgccatca
    62101 agagttgtgt tcatggtcga caacatagca ggtcgatgcc cttgagataa gatgttatag
    62161 gcgaaatagg ctgctgttgt cttaccctta gtaccagtaa aggcaaggag tttgagtttt
    62221 tcctgtggat taccatagaa ctccatggca atcaaactca tggctttctt tatatcgttc
    62281 acaatgatga cagggatacc gacttcgtag tccttttcag ctacatacca agctaagcct
    62341 tgtgttatag cagaaagaag gtattctttt ttaaaggcag cgccttttgc aaaaaaaaga
    62401 gtgtcttctg ttacttttcg gctgtcgtag ctgatgctat caaaaacaac tttgctgtag
    62461 ttgtagtggt aatgaccttg gtcaataatt tcgcgaaaaa ggccatcttt ctttaaaata
    62521 tctaatacag tttcaatttt tatcatactt tctattgtaa accgaaagtc gcaaatttac
    62581 aagtaacaag gaaaagttta taatggaaga taaggagttt ttcctagtta tcaaaattga
    62641 atgaggaatc tatgtcgcac gaaaacaatc accagcaggc ccagatgtta cgggggactg
    62701 cttggctaac ggctagtaac tttatcagtc gcctactcgg ggctgtttac attatccctt
    62761 ggtacatctg gatgggggct tatgcagcta aggcaaatgg tctctttacc atgggttaca
    62821 atatctatgc ttggttcttg ttggtttcaa cagcggggat tccagttgcg gtggccaagc
    62881 aagttgccaa gtataatacc atgcgagaag aagagcatag ctttgccctg attcggagct
    62941 tcttaggctt tatgacagga ctaggcctgg tttttgcttt agtcttgtat gtctttgctc
    63001 cttggctagc agacttgtct ggcgtgggca aagacttgat cccaatcatg caaagcttgg
    63061 cttggggagt cttgattttc ccgtctatga gtgttatccg aggatttttc caagggatga
    63121 ataacctcaa accctatgcc atgagccaaa ttgctgagca ggtcattcgt gttatctgga
    63181 tgctcctagc aacctttatc attatgaagc tcggttcagg agattatcta gcagccgtta
    63241 cccaatcaac ctttgctgcc tttgtcggta tggtagccag ttttgcagtc ttgatttatt
    63301 tccttgccca agaaggttca ctcaaaagag tctttgaaac aggagataag attaacagta
    63361 agcgtctctt ggttgatacc attaaggaag ccattccttt tatcctgaca gggtctgcca
    63421 tccagctctt ccagattttg gatcagctga cctttatcaa tagtatgagc tggtttacca
    63481 actacagcaa tgaggacttg gttgtcatgt tttcttattt ctcagccaat cctaataaaa
    63541 tcacgatgat tttgatttct gtaggggttt cgattgggag tgttggtttg ccacttttga
    63601 cggaaaacta tgtcaagggg gacttgaaag cagcttctcg tctggttcag gacagtctca
    63661 ccctactctt tatgttcttg ctaccagcaa cggttggagt ggttatggta ggagaacctc
    63721 tatatacagt cttctatggt aagccagata gtttggctct gggcttattt gtctttgcag
    63781 ttttgcagtc tattatttta ggcttgtaca tggtcttgtc tccaatgctt caggccatgt
    63841 tccgcaaccg caaggccgtt ctctatttta tctatggttc tattgccaag ctagtcttgc
    63901 aactacctac catcgccctc ttccacagtt atggtccttt gatttcaaca accattgctc
    63961 tcatcattcc taacgtcttg atgtatcggg atatttgtaa agtaactggt gtcaagcgca
    64021 aggtgatttt gaagcgaacc attttaatca gtttgctgac cctagtcatg tttctgttaa
    64081 taggaaccat ccagtggctg ttaggatttt tcttccaacc aagtggacgt ttgtggagct
    64141 tcttttatgt agctcttgtc ggtgccatgg ggggtggact ttatatggtt atgagtctgc
    64201 gtacctattt attagataag gtaataggaa aagcccaagc agatcgcctg cgagcaaaat
    64261 ttaagctttc gtaaggtagc atttataaat aaaaggaata agttgaacaa tcttttagga
    64321 gaacttgaag cttattcctt ttttagcgta aaaatagcta aaattatatt tcaaaaatag
    64381 aaaatgaggc ttttttcgat tttttggaaa aaatcattga ctaaacaaac taaacgttgt
    64441 ataataaaac aaatatttaa agcaggaggt tccggaaatg aaaaagtcta agagcaaata
    64501 tctaaccttg gcaggtcttg tcctgggtac aggagtttta ttgagcgcgt gtggaaattc
    64561 tagcacggcg tcaaaaacat acaactatgt ttattcaagt gatccatcta gcttgaacta
    64621 tctagcagaa aaccgcgcag caacatccga tattgttgca aatttggtag acgggttatt
    64681 agaaaatgac caatatggga atattattcc atcattagca gaggattgga ctgtttctca
    64741 ggacggtttg acctatacct acaaacttcg taaggatgcc aagtggttta cttctgaggg
    64801 agaagaatat gcgcctgtaa ctgcccagga ttttgtgaca ggtttgcaat atgcagctga
    64861 taaaaaatca gaagccttgt atctagtgca ggactctgtt gctggtttgg atgactatat
    64921 cactggtaaa acaagcgact tttcaactgt cggtgtcaag gcacttgatg accaaacggt
    64981 tcaatatact ttggttaaac cagaacttta ctggaattca aaaacacttg caacgatact
    65041 ttttcctgtt aatgcagatt tcctgaaatc aaaaggggat gattttggga aggcggatcc
    65101 atctagtatt ttgtacaatg gacctttctt gatgaaagca cttgtctcaa aatctgctat
    65161 tgaatataag aaaaacccta attactggga tgctaagaat gtctttgtag acgatgtgaa
    65221 attgacctac tatgatggta gcgaccaaga atcactggaa cgtaatttta cagctggtgc
    65281 ttatactacg gctcgtcttt ttcctaacag ctccagctat gaagggatta aagaaaaata
    65341 caaaaacaat atcatctata gtatgcaaaa ttcaacttca tatttcttta attttaacct
    65401 agataggaag tcttacaatt atacttctaa aacaagtgac attgaaaaga aatcgaccca
    65461 ggaagcagtt ctcaataaaa acttccgtca ggctatcaat tttgcttttg acagaacatc
    65521 ttatggggct cagtctgaag ggaaagaagg tgcaacaaag attttgcgta acctagtggt
    65581 tcctccaaac tttgtcagta tcaagggaaa agactttggt gaagttgtag cctctaagat
    65641 ggtcaactat ggtaaggaat ggcaaggtat caactttgcg gatggtcaag acccttacta
    65701 caatcctgag aaagccaagg ctaagtttgc ggaagctaag aaagaactcg aagcaaaggg
    65761 tgttcaattc ccaatccact tggataagac tgtggaagta acagataaag taggcataca
    65821 aggagttagt tctatcaaac aatcaattga atctgtttta ggttctgata atgtagtgat
    65881 tgacattcag caattaacat cagatgagtt tgacagttca ggctactttg ctcaaacagc
    65941 tgctcagaaa gactatgatt tatatcatgg cggttgggga cctgattatc aagacccgtc
    66001 aacctatctc gatattttta atactaatag tggaggagtt ctacaaaatc ttggactaga
    66061 gcctggagaa gccaatgaca aggctaaggc agttggactg gatgtctata ctcaaatgtt
    66121 ggaagaagct aataaagagc aagatccagc caaacgttat gagaaatatg ctgatattca
    66181 agcttggttg attgatagtt ctttagttct tccaagtgtt tcgcgtgggg gaacaccatc
    66241 attgagaaga accgtaccat ttgctgctgc ctatggttta accggtacaa aaggggttga
    66301 atcatataaa tacctcaaag tacaagataa gattgtcaca acagacgaat atgcaaaagc
    66361 cagagaaaaa tggttgaaag aaaaagaaga atccaataaa aaagcccaag aagaattggc
    66421 aaaacatgtc aaataaaatc taattgtaga taagtttgtg taagaagtct ttgtgcttaa
    66481 aaacgcataa tatcaggtat ataaaatcct agatgttatg cgttttgtta taggaagatt
    66541 tattagattt tatcctcaaa ttgagttatt cccgacttta tccgttgaaa tattataagg
    66601 agtatttatg aaattaaaat tattaagagt agatactaag gtgattatgg ggagtttctt
    66661 acttgttctg tctagtctac ttgctttgtt gcttcccctt atcttaaagg gtttaataga
    66721 tgggagttct attgaaaata taggttccaa agtatttcaa tcgtttttga tttttattgg
    66781 tcaagccttg ttttcttcta ttggttacta tctgtttagt caatcgggtg aaaaaagata
    66841 gcaaaaatca ggaaaaaagt gatagagggg ttgatttatg cagagaaatc cttctttgat
    66901 aagagccaaa gtggggagtt gacttctgcc attgtcaatg acatgagtgt cattcgtgag
    66961 tttttaatta cgactttccc aaatattatt ctgagtttag ttatggtact tggttccatt
    67021 gtagtcttat ttagtcttga ttggaatctt tctctacttt tattcatcac tcttccttgt
    67081 atgatgttta ttatcttgcc cctttccaat atcagtgaaa agtatagtcg tcgtttacag
    67141 gaggaaatcg gatttttaac agggcaattg actgaaaaga ttcaagaaca tgaattaatt
    67201 aagactaacc aagcagagaa aagtgtacag gatgttttgg ataattgtat tgaaagggta
    67261 caaaacaatt cactgaaatc agatagggtt acttcttttg agaccccgtt tgctctctta
    67321 tttatctttg cgactatagt tgtgatgcta acctatgggg gttatcgggt cagcgcagga
    67381 tatatatctg tgggaacctt ggtttcgttt ttgatttacc tctttcaatt acttaatcct
    67441 attagtaata tagctaattt tgtaactatt tattctagta gcaagggatc ttcagttgca
    67501 ctggagaact tgcttgcagt tcctaaagaa aaatttgagg gaggaaaatc ggtatcagga
    67561 caagggttga attttaacca tgtctatttt ggttatgatg aaaatcgacc tgtcttaaag
    67621 gatattactt gttcaatttt caaggggcaa aaaattgctt ttgttgggcc atctggatca
    67681 ggaaaatcaa cgattgtgcg tttgttagag cggttttata aaccgctttc aggagatatt
    67741 ctaatggagc aatcaagtat atatgatttt aacttaaaag aatggagaag taaaatcgct
    67801 tgggtttcac aaaataatgc agtcttatct ggcagtattc gtgacaatct ttgtctcggt
    67861 ttgaatcgct tagtaactga tgatgaattg atgaaagtgc tagacttagt atcactaggt
    67921 gatgagattc gctccatgaa agagggacta gatactgaag ttggtgaacg cggacgactc
    67981 ttgtcagggg ggcgaagcca aagacttcaa atagctagag cctacttaaa agatgctgaa
    68041 attcttatat ttgatgaagc cactgctaat cttgatgcgg attctgagta tgcgattatc
    68101 agtagcctct attctgtact aaaggagaag acggttgtga ttatagcgca tcgcttgtca
    68161 acggtaaaag atgtggattg tattttcttc ttagaggagg ggaaaatcac tggctcagga
    68221 actcataagg aactactgga aaatcatgag cgttatgctc gttttgtgca ggagcaaatg
    68281 atagagtgaa ggtgtctttt gagattcacc attttatagt ctattaaagg gagcaggaaa
    68341 aactcccttt ttatatagtt tgaaactata actagctctt gaaaagaaga aaatgagttg
    68401 atgaaaataa gtggtacaat agttactata gatttggagg aattgtatga gcaaggaatt
    68461 acacattaac acaattttag cacaagcagg gatcaagtct gatgaggcaa caggtgcttt
    68521 ggtgacacca cttcattttt caacgaccta tcagcatcca gagtttggtc gatctactgg
    68581 gtttgactat acgcgcacta aaaatccaac tcgtagtaag gctgaggaag tcttggcggc
    68641 tattgagtca gcagactatg ccctagcgac tagctcaggg atgtcagcta ttgtactggc
    68701 ctttagcgtc tttccagtag gaagtaaggt cttggcagtg cgtgatcttt acggtggttc
    68761 ttttcgctgg tttaaccaag tggagcagga aggtcatttc cattttacct atgccaatac
    68821 agaagaagag ttgattgccg agttagaaaa ggatgtggat gttctctata tcgaaacccc
    68881 aaccaatccc ttgatgttgg aatttgatat cgaaaaacta gcaaaattgg ctcatgctaa
    68941 gggtgccaaa gtggtggtgg acaatacctt ctatagccct atctaccaac gtccgattga
    69001 agatggagca gatatcgttc tccattcagc aaccaagtat ctagcaggcc acaatgatgt
    69061 cttggctgga gtggttgtga ccaatagttt agaactatac gagaagcttt tttacaatct
    69121 taatacaaca ggggcagtct tgtctccatt tgacagctac cagttgcttc gtggtctcaa
    69181 gaccttgtct cttcgtatgg agcgctcaac agctaacgcc caagaagtgg ttgccttttt
    69241 gaaggattct ccagcagtta aggaagttct ctacactggt cgtggaggca tgatttcctt
    69301 taaagtagcc gatgaaacac gcattcctca tattttgaac agtctcaagg tcttctcttt
    69361 tgcggaaagt ttgggtggag tggaaagtct tattacttat ccaacgactc aaactcatgc
    69421 tgatattcca gcagaagtac gccattctta tggtttgaca gatgacctct tgcgtttgtc
    69481 tattgggatt gaggatgcta gagatttgat tgcagatttg cgccaagcct tagaaggata
    69541 agacaaagat gggaaaatat gattttacaa gcctgcccaa ccgtttaggg caccatacct
    69601 ataaatggaa agaaacagaa acggatagtg aagttctacc agcttggata gcggatatgg
    69661 actttgtagt cttgcctgaa atccaccaag ccgtgcaaac ttacgcagac caactggttt
    69721 atggttatac ctatgccagt gaagacttaa ttaaggaagt tcaaaagtgg gaagctacac
    69781 aatacggtta caactttgac aaagaggctc ttgtctttat cgagggtgtg gtaccagcca
    69841 tctcaacagc tattcaaacc tttacaaaag aaggcgaggc ggttttaatt aacacgcctg
    69901 tctacccacc ctttgctcgc agtgtcaagt tgaataatcg tagattgatt actaattcct
    69961 tagtggaaaa ggatagtctg tttgagattg actttgacca acttgaaaag gatttggtgg
    70021 aagaggaggt taaactctat gttctttgca accctcacaa tcctggtgga cgtgtttggg
    70081 aaaaagaagt gttggagaag attggccaac tctgccaaaa acacggtgtt ttgttagttt
    70141 cggatgagat tcaccaagat ttgaccctct ttggtcacaa acaccagtct tttaatacca
    70201 tcaatcctgc cttcaaaaat tttgctatcg tcttgagcag tgccactaaa acatttaata
    70261 ttgctggaac aaaaaattcc tatgcagtca ttgaaaatcc taagttgaga ctggctttcc
    70321 agaaacgcct gttggccaat aatcagcatg aaatttcagg cttgggttat ttggcgacag
    70381 aagctgccta tcgatacggt aaagattggc tagaggaact caagcaagtc tttgaagacc
    70441 acatcaatta tgtggtggat ctatttggaa aagagactaa aatcaaggtc atgaaaccgc
    70501 aaggtaccta cttgatttgg cttgactttt cagcttatga cctgactgat gaaacattgc
    70561 aagagttgtt gagaaatgaa gccaaggtta tcctcaaccg tggtttggat tttggagagg
    70621 aaggaagtct ccatgcccgc atcaatatag ctatgcccaa atctctgttg caagaagtct
    70681 gtcagcggat tgtggctact tttgccaaat gttaaaaatc cagccttcta ggagaaaagt
    70741 cttcctagaa ggctattttc ataggcgaaa atatggtata ataaaaagat aaggtaaagg
    70801 tgaaaatatg gctaaattga ttccggggaa aattcgtatc gaaggtgttg ccctttatga
    70861 aactggtaag gttgatatca tcaaggaaaa gaacaatcgg ctctacgctc gcgttgcaga
    70921 agaagaactg cgctatagtt tagaggatga tttgattttt tgtgcctgtg atttttttca
    70981 aaagaggggc tactgtgtgc atttggcagc gctagagcat tttctgaaaa atgatgagcg
    71041 tggtcaggaa atcttgtgga gtctggaaga aggtcatgaa gaaaaagagg ccgttgaaac
    71101 caaggtgacc ttgggtggca agtttttgga tcgaatttta tctccaaaat cagaatgcgc
    71161 ctatgagtta tcagctgtgg gtcaggtgga agcaggaacc aatcatatct tgtggactct
    71221 gagaatcggc caaatcaata gccaaaaata ctatgtcatt cgggatattc cacttttttt
    71281 aaggattgtc gagcagagaa agtcgtatat gattggcaag atttatgagg agtctctttc
    71341 ttgggaagcc tttgatgatg ccagccaaga acttttgact ttcttgcacg gactaacgga
    71401 ggaagggctg gttccagacc tctttttcca aaaccaaggt cggcacctct ttttccctct
    71461 gacctttttt gagcagggtg tgaatttact gatggccttg ccccattttc aatttgacca
    71521 tcaagtggat agctaccaaa ccctgctttt tcaggatatg catgctgatg ccaatctatt
    71581 tgcctttaca gtaacagaat attcggacta ttttgaaatg gaaatcagtg agagtcctag
    71641 ggtcaatgtc ttttatcaag gagcagtact tttccataaa ggtcaggttt attttttaac
    71701 agaccagcag atgcgtcttc tcaaggaaat taaagcgtta cctgtggatc gacaaggaaa
    71761 gaaatacctg caatttgata gcagtgatcg agacaaattg gcctcctgtc tgactctttt
    71821 tatccagatg ggcaccgttt cagctccaga acgtctacaa atcaaaactt ttgcgccctc
    71881 tttttacttt gacagggaag aagataatcg gattcgttta gaaattcagt ttgattatgg
    71941 aaatagacag gtatctagcc gacaagagct agaagaatta ccattttcaa gtgacgcgga
    72001 cttagaagaa agaattttcc aagtctgttt ggctgcgggc tttgaagccg attttcaatc
    72061 ttggcgtcag gccttaaaag cggagtctgt ctatcatttt tttcatgata tcattccagt
    72121 ctttgaaaaa ctcgggcatg ttgacctatc agataaacta gaagagcttt atagcctagc
    72181 gagtcctcag gtgcagattg cctccaaggg aggtctcttg gaaatccagt ttgattttca
    72241 agatattgcc caggaggaaa tcgaccaagc catacaggcc ttggttgcca atcaggattt
    72301 ttatattgat tcgtctaatc aagtttactt tttcgatgaa gaaaccaaga aaattcgcca
    72361 aaatctacag gaactggggc aatttgaatt aaaagatggg accttgcagg ctcggaaatc
    72421 cttggcctac agtttagctc atctctttga agggcgagac cgtgtttctt tttcacaaga
    72481 attccagaat ctggcccagg atttgacgca tccagaggac tttcctttgc aagcaactca
    72541 ggtcaaggct gacttgcgag attatcagga aaagggaatt ggctggttgc agatgctcca
    72601 tcattatggt tttggtggga ttttggctga tgatatggga cttgggaaaa cccttcagac
    72661 cattgctttt ttgactagtc aagtgacaaa agaaagtcgg gttttgattc tcgctccgtc
    72721 gggtttgatt tacaactggg cagatgagtt tcagaaattt gctccacagt tggatgtggc
    72781 tgttgttcat ggtttgaaag caagtcgtga ggagattctt gccgagagcc atcaaatcta
    72841 tgtgactagc tatgccacct tccgtcagga cagtgagctt tatcagggga tggcctttga
    72901 cttccttttc ttagatgaag ctcaggtcat gaaaaatgcc cagaccaaga ttgcccagac
    72961 cttgagacaa tttgtggtgc cgtcggtctt tgccttgtca ggaactccga ttgaaaacca
    73021 tctgggtgag ttgtggtcta tttttcaaat cgtcatgccc ggacttttgc caagcaagaa
    73081 agaatttatg aaattgccag cagagcgagt ggctcagttt atcaagcctt tcgtgatgcg
    73141 acgcaagaaa gaagaagttc tgactgaatt gccagacttg attgaggtgg tttataaaaa
    73201 tgaactggaa gaccagcaaa aggctattta ccttgcccag ttacaacaga tgcgagacca
    73261 tctggctcaa gtgtcagaac aggaatttca gcgaagtcgt gtggaaattt tatctggttt
    73321 gatgcgcttg cgtcaaatat gtgacactcc tgccctgttt atggaagatt atcagggagc
    73381 cagcggcaaa ctagatagtc tccgagacct actggtacag gtggcagacg gcggacaccg
    73441 tgtcttgatt ttctctcagt tcaagggaat gttggaaaaa attgaacaag aactgccaga
    73501 cttgggcctg acatccttta aaattacggg ttcaacccca gccaaggaaa gacaagacat
    73561 gaccaaggcc tttaaccaag gagaaagaga tgcctttctg atttccctca aggctggtgg
    73621 ggtcggtctg aacctgacag gtgcagatac agtgatcttg gttgaccttt ggtggaatcc
    73681 tgcggtggaa gcgcaagcca ttggccgtgc ccatcggatg ggtcaggaag aaacggttga
    73741 ggtctatcgc ttggtgacca agggaaccat tgaagaaaaa attcaggaac tccaagaaca
    73801 aaagaaacat ctggtgtcac aagtattgga cggcacagag tcacgtggta gtctgaccct
    73861 agcagaaatt agagaaattt tgggaatttc tgaagccagc acttgaaaaa tcaagacaat
    73921 acgctataat gaagtgttga aaggaaatag aaaatgaaac aagaacgatt tccattggtg
    73981 tcagatgacg aggtcatgtt gactgaaatg ccagtcatga atctctatga tgagtctgat
    74041 ctgatcagta atatcaaggg tgagtatcga gataaaaatt atttagaatg ggctcctatt
    74101 gctgaagaaa aaccagtaaa accgattgaa aagcaagtag ggaaacctaa aaaagctcct
    74161 ttaggggtta aaaaagaagg aaagagctat gcggaggtgg cgcgtgaaga agcgcgtgca
    74221 gacttgaaaa agaaacgctc tgctaactat ctaactcagg atttcagcct tgcgagacgt
    74281 cattctcagc ccagtctagt tagacagggc aatcaaccga caactccttt ccaaaaggaa
    74341 aatcctggtg aatttgtcaa atatagccaa aaattgaccc agtctcatta tatcttggcg
    74401 gaagaagttc attctatccc taccaagaat gaagaagtgt cagcacctgc tccaaagaaa
    74461 aacaattatg attttctaaa gaagagccaa atctacaata aaaaaagtaa acaaacagaa
    74521 caagaacgtc gggttgccca agagttgaat ctgaccagaa tgacagaata ggggagaaaa
    74581 catgtcaaag acatatcatt ttatcggaat taagggatca gggatgagtg ccttggcctt
    74641 gatgttgtac cagatggggc acaaggttca gggatcagat gttgaaaagt actactttac
    74701 ccaacgcggt cttgagcagg caggaattac cattcttcct tttgatgaaa agaatctaga
    74761 cggtgatatg gaaattatcg ctggaaatgc ctttcgtcca gataacaacg tcgaaattgc
    74821 ctatgcggac caaaatggta tcagctacaa acgttaccat gagtttctag gtagctttat
    74881 gcgtgacttt gttagcatgg gagtagcagg agcacatgga aaaacttcaa cgacaggtat
    74941 gttgtctcat gtcttgtctc acattacaga taccagcttc ttgattggag atgggacagg
    75001 tcgtggttcg gccaatgcca aatattttgt ctttgaatct gacgaatatg agcgtcactt
    75061 catgccttac cacccagaat actctattat caccaacatt gactttgacc atccagatta
    75121 tttcacaagt ctcgaggatg ttttcaatgc ctttaacgac tatgccaaac aaatcaccaa
    75181 gggtcttttt gtctatggtg aagatgctga attgcgtaag attacgtctg atgcaccaat
    75241 ttattattat ggttttgaag ctgaaggcaa tgactttgta gctagtgatc ttcttcgttc
    75301 aacaactggt tcaaccttca ccgttcattt ccgtggacaa aacttggggc aattccacat
    75361 tccaaccttt ggtcgtcaca atatcatgaa tgcgacagcc gttattggtc ttctttacac
    75421 agcaggattt gatttgaact tggtgcgtga gcacttgaaa acattttccg gtgttaaacg
    75481 tcgtttcact gagaaaattg tcaatgatac agtgattatc gatgactttg cccatcatcc
    75541 aacagaaatt attgcgacct tggatgcggc tcgtcagaaa tacccaagca aggaaattgt
    75601 agcagtcttt caaccgcata cctttacaag aaccattgcc ttgttggacg actttgccca
    75661 tgctttaaac caagcagatg ctgtttatct agcgcaaatt tatggctcgg ctcgtgaagt
    75721 agatcatggt gacgttaagg tagaagacct agccaacaaa atcaacaaaa aacaccaagt
    75781 gattactgtt gaaaatgttt ctccactcct agaccatgac aatgctgttt acgtctttat
    75841 gggagcagga gacatccaaa cctatgaata ctcatttgag cgtctcttgt ctaacttgac
    75901 aagcaatgtt caataggatg ttcccatgga aattccaatt aagatcattc aggcaagcaa
    75961 gtctgatttg cctgagatag gggcacttca gacctcgtct tttccagctg aaaagcagca
    76021 actttcccat attttagaag aaagtatccg taagtgtgcg gatacctttc ttctagctag
    76081 ggatgaaaat caacttttag gctatatttt atcaagtccc cagtcagaca atccgcaata
    76141 tctaaaagta cattctttag tcatcgagtc tgaccatcag agacagggct tgggaacact
    76201 tcttcttgca gccttgaaag aggtggcagt tgagctggat tacaaaggga ttcgtttgga
    76261 gagtcctgat gagctgcttt cctattttga aatgaacggt tttgttgatg aagaagcaac
    76321 tttgctctat gcaactagcc agggctatag tatgatttgg tttaatccct tttatctgga
    76381 ggaacaatga aaatcagaca agcaagatta gaagatttgg atcggattgt tgaacttgaa
    76441 tttgaaaatt tttcggtcga agaagccatt cctccttctg tctttgaagc acatttgaga
    76501 gaaattcaga cctcttttct ggttgctgaa aaagaaggaa gaatcatggg ttatatcgaa
    76561 ggaccagttg gcctgcaccg ccatctgcaa gaccagtctt ttacagaaga aataaaagac
    76621 tatagtcatg agcctggtgg ttatatctct gtgacctgtc tgtctattgc caaggaggca
    76681 cagggactcg gactgggtca gaaattgctg acagccttga aagaagttgc tcttgaagat
    76741 gagagagatg gcattaatct aacctgtcat gactatctca tcgcctacta tgaaaaacat
    76801 ggatttgtca acgaaggcca gtcccagtca acctttgcag gggaaacatg gtatgatatg
    76861 gtctgggaaa tgaaaaaata agttaggaaa agtatcatag attgcttttt ttggactttt
    76921 aagatataat attatggatt atcaacaagg aggaaaaact tttgagtgaa aagtcaagag
    76981 aagaagagaa attaagcttt aaagagcaga ttctgagaga tttagaaaaa gtaaaaggct
    77041 atgatgaagt tctgaaagaa gatgaggcag tagttcgcac tcctgcaaat gaaccttcaa
    77101 ctgaagaact catggctgat tccttgtcaa cggtagagga gattatgaga aaagctccta
    77161 ccgtgcctac tcacccaagt caaggtgtac cagcttctcc agcagatgag attcaaagag
    77221 aaactcctgg tgttccaagt catccaagtc aagatgtacc ttcttctcca gcggaagaaa
    77281 gtggatcaag accaggtcca ggtcctgtta gacctaagaa acttgaaaga gaatacaatg
    77341 aaaccccaac aagggtagct gtttcctata cgacggcaga gaaaaaagca gaacaagcag
    77401 gtccagaaac acctacgcct gctacagaaa cagtggatat catcagagat acatcacgtc
    77461 gtagccgtag agaaggagca aaacccgtta agcctaagaa agagaagaag tcacatgtga
    77521 aagcttttgt gatttcattc cttgtattcc ttgccttgct ctcagcaggt ggttactttg
    77581 gttaccagta cgtgctagat tccttattac ctatcgatgc taattctaag aaatatgtga
    77641 cggttggaat tccagaaggt tcaaacgttc aagaaatcgg tacgacgctt gaaaaagctg
    77701 gtttggtaaa gcatggtctg atttttagtt tttatgccaa gtataaaaat tataccgact
    77761 tgaaagcagg ttactacaat ttgcaaaaga gtatgagtac agaagactta ctcaaagagt
    77821 tgcaaaaagg tggaacagat gaaccgcaag aacctgtact tgcgactttg acaattccag
    77881 aaggttatac cttggatcag attgctcaag ctgtgggtca attgcaaggt gacttcaaag
    77941 agtctttgac agcggagact ttcttggcta aagttcaaga tgagacgttt atcagtcaag
    78001 cagtagcgaa atatcctact ttactggaaa gtttgcctgt aaaagacagc ggtgcgcgtt
    78061 atcgtttgga aggatacctt ttcccagcta catactctat caaggaaagc acaactattg
    78121 agagcttgat tgatgagatg ttagctgcta tggataagaa cctatctcct tactatagta
    78181 ctatcaaatc taaaaacttg actgtcaatg agttgttgac cattgcttcc ttggtcgaaa
    78241 aagaaggtgc caagacagaa gatcgtaagc tcattgcagg tgtattctac aatcgtttga
    78301 atcgtgatat gccacttcaa agtaatattg caatcttgta tgcccaagga aaactggggc
    78361 aaaatatcag tctagctgag gatgttgcga ttgataccaa cattgattca ccttataatg
    78421 tttataaaaa tgtaggtctc atgcctggtc cagtcgatag tccaagtctg gatgcgattg
    78481 agtcaagcat caatcaaact aagagcgata acctctactt tgtagcagat gtcacagaag
    78541 gcaaggtcta ctatgctaac aatcaagaag accacgaccg caatgtcgct gaacatgtca
    78601 acagcaaatt aaactaaaca aactaaaatt atgttatact tcacataatt ttctttagaa
    78661 aatattatca gaagaaagtt gagaaaaatg gcagaaaaaa catatcctat gacccttgag
    78721 gaaaaggaaa aacttgaaaa agaattagaa gaattgaaat tggttcgtcg accagaagtg
    78781 gtagaacgca ttaagattgc ccgttcatac ggtgaccttt cagaaaacag tgagtacgaa
    78841 gcagctaagg atgaacaagc ctttgtcgaa ggacaaatct ctagcttaga aacaaaaatc
    78901 cgctatgctg aaatcgtcaa tagcgacgca gttgcccagg acgaagtagc gattggtaaa
    78961 acagtcacca tccaagaaat tggtgaggac gaagaagaag tttatattat cgtaggttca
    79021 gctggtgcgg atgcctttgc aggtaaggtt tcaaatgaaa gcccaattgg gcaggccttg
    79081 attggcaaga aaacaggtga tacagcaacc attgaaacgc ctgttggtag ctatgatgta
    79141 aaaatcttga aggttgaaaa aacagcctaa aaacagaaaa aggagtgggg aggcgatgtg
    79201 cttcactcac tcctttttcc attttgctat tcttcgaaaa tctcttcaaa ccacgtcagc
    79261 gtcgccttgc cgtatgtatg gttactgact tcgtcagttt catctacaac ctcaaaacca
    79321 tgttttgagc cgacttcgtc agtttcatct acaacctcaa aaccatgctt tgagctgact
    79381 tcgtcagttc tatctacaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta
    79441 gtttgctctt tgatttttat tgagtattag atgtatagtg cattgaagtt aaaatagtac
    79501 actttgattt ctaagacatt gttagaaatt agattaaatt ccttaatcga tttgcgcatg
    79561 ttttatttca tttcactata ctttagattg gaaaataagg aatcacataa atcgcaagaa
    79621 atagggaatc tgattcgtgt ttataaccgt tcaaaaagag aagaggctga aagtgagcca
    79681 cttaatcttt atgtcgaaga tgaaaagggc aatctcctgg caggtttgat agcagagact
    79741 tttggaaatt ggctagaaat cgagtatttg tttgtaaaag aggaactgcg agggcaagga
    79801 atcggttcaa aactattgca acaggcagaa agtgaagcta agaatcgaaa ttgttgtttt
    79861 gcctttgtca atacttacca gttccaggca ccggactttt atcagaaaca tggctacaag
    79921 gaagtttttt ctttgcaaga ctatctctac attaggcaaa gatattatta ccaaaagaat
    79981 ctgtaaggtg agtatggagg tataatacaa agagaggctc gcacataagt atggaggtgg
    80041 actgatgtat atgagtaact atcgataaat accagttata acctagaaat tgtgtctttc
    80101 tagacgatat tgaggacaat ataatcgtag ccccaaaatt aggaatcaag acttatcagg
    80161 ttaagaaaag aagtgatgtt gttgatattt taaacaattt atttaaacta aaaactctct
    80221 atctattaat ccgagattcc cctctcggat tttgtgttgc tttcattaaa tttacaggta
    80281 agacttgcta gcttcagtaa atatacacaa gtttttcaga atagagtgaa atccaaaaga
    80341 aaatggaaat aatgaaagct cttgtaggca taagaaaaca cctctgtgtt atacttgttg
    80401 ttcaaccaca aacacaagaa aggcacagag atgcaagaca attatactac aaaagccaaa
    80461 catttgacaa tcgatagccg tcgcttaatc gaaagatgga aaaaagaagg gaaatcaaat
    80521 agagaaattg cctctctgct tggaaaagtt cctcaaacta tccacactga aatcaagcgt
    80581 gggacagtcc gacaatgtct tggaaaaggg cgcttcaaag aggtttattc tgccgactat
    80641 gctcaacagt cttatgaaaa taatcgcaag cgctcggtca agaaatcaag cttgaccaag
    80701 gaactaaagg aaaagattct ccactatcat aaccaaaaat tttcgcctga aatgatggtg
    80761 atggctaaag gggttaacgt gggaatttca accatttact attggattca tcatggaaaa
    80821 ttggggttaa gcaa
//
LOCUS       DBEJAQ010000003        77220 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.003, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000003 DBEJAQ010000000
VERSION     DBEJAQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 77220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.003"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            371..598
                     /locus_tag="ACL297_000227"
     CDS             371..598
                     /locus_tag="ACL297_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070554.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            886..3783
                     /gene="nanA"
                     /locus_tag="ACL297_000228"
     CDS             886..3783
                     /gene="nanA"
                     /locus_tag="ACL297_000228"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HGR0070555.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNNQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGNPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKDPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            4798..5250
                     /locus_tag="ACL297_000229"
     CDS             4798..5250
                     /locus_tag="ACL297_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGR0070556.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            5269..6606
                     /locus_tag="ACL297_000230"
     CDS             5269..6606
                     /locus_tag="ACL297_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0070557.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            6685..7569
                     /locus_tag="ACL297_000231"
     CDS             6685..7569
                     /locus_tag="ACL297_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGR0070558.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            7569..8402
                     /locus_tag="ACL297_000232"
     CDS             7569..8402
                     /locus_tag="ACL297_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGR0070559.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            8420..10513
                     /gene="nanB"
                     /locus_tag="ACL297_000233"
     CDS             8420..10513
                     /gene="nanB"
                     /locus_tag="ACL297_000233"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HGR0070560.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            10525..11628
                     /locus_tag="ACL297_000234"
     CDS             10525..11628
                     /locus_tag="ACL297_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGR0070561.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            11802..12500
                     /locus_tag="ACL297_000235"
     CDS             11802..12500
                     /locus_tag="ACL297_000235"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGR0070562.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            12519..14036
                     /locus_tag="ACL297_000236"
     CDS             12519..14036
                     /locus_tag="ACL297_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGR0070563.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            14161..15489
                     /locus_tag="ACL297_000237"
     CDS             14161..15489
                     /locus_tag="ACL297_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0070564.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            15741..16607
                     /locus_tag="ACL297_000238"
     CDS             15741..16607
                     /locus_tag="ACL297_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGR0070565.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAIMIAIVSF
                     VQFKVLGNDVEY"
     gene            16623..17462
                     /locus_tag="ACL297_000239"
     CDS             16623..17462
                     /locus_tag="ACL297_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGR0070566.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            17734..18186
                     /locus_tag="ACL297_000240"
     CDS             17734..18186
                     /locus_tag="ACL297_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGR0070567.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNRFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            18283..18627
                     /locus_tag="ACL297_000241"
     CDS             18283..18627
                     /locus_tag="ACL297_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070568.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVTALIGRYIKLGTY
                     AVKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            18637..18831
                     /locus_tag="ACL297_000242"
     CDS             18637..18831
                     /locus_tag="ACL297_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070569.1"
                     /translation="MKALCIFLLYMLAWTVLAMIIRGGLDGFDEHTWRTILIASLSGI
                     YIDYMSIDKSRKKSKRKKKK"
     gene            18914..19558
                     /locus_tag="ACL297_000243"
     CDS             18914..19558
                     /locus_tag="ACL297_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGR0070570.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            19727..20644
                     /locus_tag="ACL297_000244"
     CDS             19727..20644
                     /locus_tag="ACL297_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGR0070571.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEDDRPVVEAAAALIRETKERFL"
     gene            20662..21546
                     /locus_tag="ACL297_000245"
     CDS             20662..21546
                     /locus_tag="ACL297_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGR0070572.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(21562..22413)
                     /locus_tag="ACL297_000246"
     CDS             complement(21562..22413)
                     /locus_tag="ACL297_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGR0070573.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            22613..24670
                     /gene="pbp2b"
                     /locus_tag="ACL297_000247"
     CDS             22613..24670
                     /gene="pbp2b"
                     /locus_tag="ACL297_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HGR0070574.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            24681..25277
                     /gene="recR"
                     /locus_tag="ACL297_000248"
     CDS             24681..25277
                     /gene="recR"
                     /locus_tag="ACL297_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGR0070575.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            25453..26496
                     /locus_tag="ACL297_000249"
     CDS             25453..26496
                     /locus_tag="ACL297_000249"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGR0070576.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            26580..27953
                     /gene="murF"
                     /locus_tag="ACL297_000250"
     CDS             26580..27953
                     /gene="murF"
                     /locus_tag="ACL297_000250"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGR0070577.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKNYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            27940..28551
                     /locus_tag="ACL297_000251"
     CDS             27940..28551
                     /locus_tag="ACL297_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HGR0070578.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            28642..29346
                     /locus_tag="ACL297_000252"
     CDS             28642..29346
                     /locus_tag="ACL297_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HGR0070579.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            29565..30938
                     /gene="ftsA"
                     /locus_tag="ACL297_000253"
     CDS             29565..30938
                     /gene="ftsA"
                     /locus_tag="ACL297_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGR0070580.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            30955..32214
                     /gene="ftsZ"
                     /locus_tag="ACL297_000254"
     CDS             30955..32214
                     /gene="ftsZ"
                     /locus_tag="ACL297_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGR0070581.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            32219..32890
                     /locus_tag="ACL297_000255"
     CDS             32219..32890
                     /locus_tag="ACL297_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGR0070582.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            32900..33439
                     /locus_tag="ACL297_000256"
     CDS             32900..33439
                     /locus_tag="ACL297_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGR0070583.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            33439..33702
                     /locus_tag="ACL297_000257"
     CDS             33439..33702
                     /locus_tag="ACL297_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGR0070584.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            33699..34484
                     /locus_tag="ACL297_000258"
     CDS             33699..34484
                     /locus_tag="ACL297_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HGR0070585.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            34493..35281
                     /gene="divIVA"
                     /locus_tag="ACL297_000259"
     CDS             34493..35281
                     /gene="divIVA"
                     /locus_tag="ACL297_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HGR0070586.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            35629..38421
                     /gene="ileS"
                     /locus_tag="ACL297_000260"
     CDS             35629..38421
                     /gene="ileS"
                     /locus_tag="ACL297_000260"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGR0070587.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            38824..39204
                     /locus_tag="ACL297_000261"
     CDS             38824..39204
                     /locus_tag="ACL297_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070588.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
     gene            39398..39688
                     /locus_tag="ACL297_000262"
     CDS             39398..39688
                     /locus_tag="ACL297_000262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070589.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            39739..40431
                     /locus_tag="ACL297_000263"
     CDS             39739..40431
                     /locus_tag="ACL297_000263"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HGR0070590.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            40484..42895
                     /locus_tag="ACL297_000264"
     CDS             40484..42895
                     /locus_tag="ACL297_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGR0070591.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAVRASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            43145..43846
                     /locus_tag="ACL297_000265"
     CDS             43145..43846
                     /locus_tag="ACL297_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070592.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            43848..46556
                     /locus_tag="ACL297_000266"
     CDS             43848..46556
                     /locus_tag="ACL297_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGR0070593.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGIVVGETQIQFYWTYSLLALVLSWLASVLPVYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSETPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(46652..47143)
                     /gene="tpx"
                     /locus_tag="ACL297_000267"
     CDS             complement(46652..47143)
                     /gene="tpx"
                     /locus_tag="ACL297_000267"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGR0070594.1"
                     /translation="MVTFLGNPVSFTGKQLQGGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(47266..48195)
                     /gene="psaA"
                     /locus_tag="ACL297_000268"
     CDS             complement(47266..48195)
                     /gene="psaA"
                     /locus_tag="ACL297_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HGR0070595.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(48227..49075)
                     /locus_tag="ACL297_000269"
     CDS             complement(48227..49075)
                     /locus_tag="ACL297_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HGR0070596.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(49072..49794)
                     /locus_tag="ACL297_000270"
     CDS             complement(49072..49794)
                     /locus_tag="ACL297_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGR0070597.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            50085..51977
                     /gene="pepO"
                     /locus_tag="ACL297_000271"
     CDS             50085..51977
                     /gene="pepO"
                     /locus_tag="ACL297_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HGR0070598.1"
                     /translation="MTRYQDDFYDAINGKWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLEFDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(52100..52729)
                     /locus_tag="ACL297_000272"
     CDS             complement(52100..52729)
                     /locus_tag="ACL297_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGR0070599.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(53069..53311)
                     /locus_tag="ACL297_000273"
     CDS             complement(53069..53311)
                     /locus_tag="ACL297_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070600.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            53722..55944
                     /locus_tag="ACL297_000274"
     CDS             53722..55944
                     /locus_tag="ACL297_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HGR0070601.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            55974..56417
                     /gene="dtd"
                     /locus_tag="ACL297_000275"
     CDS             55974..56417
                     /gene="dtd"
                     /locus_tag="ACL297_000275"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGR0070602.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            57208..57591
                     /locus_tag="ACL297_000276"
     CDS             57208..57591
                     /locus_tag="ACL297_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070603.1"
                     /translation="MGKENVRMRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVR
                     EYVPKKERIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQ
                     AKRIHRAFCIRLCLRAQFLLSNNDS"
     gene            57999..58298
                     /locus_tag="ACL297_000277"
     CDS             57999..58298
                     /locus_tag="ACL297_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070604.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(58393..58830)
                     /locus_tag="ACL297_000278"
     CDS             complement(58393..58830)
                     /locus_tag="ACL297_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070605.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(58961..59482)
                     /locus_tag="ACL297_000279"
     CDS             complement(58961..59482)
                     /locus_tag="ACL297_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0034258 - nicotinamide riboside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HGR0070606.1"
                     /translation="MLSTRFTGKLSKWENYFGIVNTILSGAIDYILGNKAAIITYPVT
                     FLIYTFAIKKWKASQEGRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLA
                     YVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNA
                     IGALFVWNDEEVR"
     gene            complement(59806..60456)
                     /locus_tag="ACL297_000280"
     CDS             complement(59806..60456)
                     /locus_tag="ACL297_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HGR0070607.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            60568..61410
                     /locus_tag="ACL297_000281"
     CDS             60568..61410
                     /locus_tag="ACL297_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGR0070608.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            61486..61923
                     /locus_tag="ACL297_000282"
     CDS             61486..61923
                     /locus_tag="ACL297_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGR0070609.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            61991..62293
                     /locus_tag="ACL297_000283"
     CDS             61991..62293
                     /locus_tag="ACL297_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070610.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(62384..63457)
                     /locus_tag="ACL297_000284"
     CDS             complement(62384..63457)
                     /locus_tag="ACL297_000284"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HGR0070611.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            63613..64290
                     /locus_tag="ACL297_000285"
     CDS             63613..64290
                     /locus_tag="ACL297_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGR0070612.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            64283..65257
                     /locus_tag="ACL297_000286"
     CDS             64283..65257
                     /locus_tag="ACL297_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGR0070613.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(65306..67249)
                     /gene="thrS"
                     /locus_tag="ACL297_000287"
     CDS             complement(65306..67249)
                     /gene="thrS"
                     /locus_tag="ACL297_000287"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGR0070614.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(67307..67630)
                     /locus_tag="ACL297_000288"
     CDS             complement(67307..67630)
                     /locus_tag="ACL297_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGR0070615.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            complement(68061..68261)
                     /locus_tag="ACL297_000289"
     CDS             complement(68061..68261)
                     /locus_tag="ACL297_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070616.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            68499..69128
                     /locus_tag="ACL297_000290"
     CDS             68499..69128
                     /locus_tag="ACL297_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HGR0070617.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            69269..69538
                     /gene="rpsO"
                     /locus_tag="ACL297_000291"
     CDS             69269..69538
                     /gene="rpsO"
                     /locus_tag="ACL297_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGR0070618.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            70353..70967
                     /locus_tag="ACL297_000292"
     CDS             70353..70967
                     /locus_tag="ACL297_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HGR0070619.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(71260..72009)
                     /locus_tag="ACL297_000293"
     CDS             complement(71260..72009)
                     /locus_tag="ACL297_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGR0070620.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            72142..74478
                     /locus_tag="ACL297_000294"
     CDS             72142..74478
                     /locus_tag="ACL297_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGR0070621.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            74580..75257
                     /locus_tag="ACL297_000295"
     CDS             74580..75257
                     /locus_tag="ACL297_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGR0070622.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            75244..76041
                     /locus_tag="ACL297_000296"
     CDS             75244..76041
                     /locus_tag="ACL297_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGR0070623.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            76058..77161
                     /locus_tag="ACL297_000297"
     CDS             76058..77161
                     /locus_tag="ACL297_000297"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGR0070624.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDNLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
BASE COUNT        23391 a        13893 c        17182 g        22754 t
ORIGIN      
        1 gtcagtctta tctacaacct caaaacagtg ttttgagcat catgcggcta gcttcttagt
       61 ttgctctttg attttcattg agtataaaaa cagatgagtt tctgttttct ttttatggac
      121 tataaatgtt cagctgaaac tactttcaag gacattatta tataaaagaa ttttttgaaa
      181 ctaaaatcta ctatattaca ctatattgaa agcgttttaa aaatgaggta taataaattt
      241 actaacgctt ataaaaagtg atagaatcta tttttatgta tatttaaaga tagattgctg
      301 taaaaatagt agtagctatg cgaaataaca gatagagaga agggattgaa gcttagaaaa
      361 ggggaataat atgatattta aggcattcaa gacaaaaaag cagagaaaaa gacaagttga
      421 actacttttg acagtttttt tcgacagttt tctgattgat ttatttcttc acttatttgg
      481 gattgtcccc tttaagctgg ataagattct gattgtgagc ttgattatat ttcccattat
      541 ttctacaagt atttatgctt atgaaaagct atttgaaaaa gtgttcgata aggattgagc
      601 aggaagtatg gtgtaaatag cataggctga tgtccatcat ttgcttataa agagatattt
      661 tagtttaatt gcagcggtgt cctggtagat aaactagatt ggcaggagtc tgattggaga
      721 aaggagaggg gaaaattggc accaatttga gatagtttgt ttagttcatt tttgtcattt
      781 aaatgaactg tagtaaaaga aagttaataa aagacaaact aagtgcattt tctggagtaa
      841 atgtcttatt tcagaaatcg ggatatagat atagagagga tcagtatgaa tcggagtgtt
      901 caagaacgta agtgtcgtta tagcattagg aaactatcgg taggagcggt ttctatgatt
      961 gtaggagcag tggtatttgg aacgtctcct gttttagctc aagaaggggc aagtgagcaa
     1021 cctctggcaa atgaaactca actttcgggg gagagctcaa ccctaactga tacagaaaag
     1081 agccagcctt cttcagagac tgaactttct ggcaataatc aagaacaaga aaggaaagat
     1141 aagcaagaag aaaaaattcc aagagattac tatgcacgag atttggaaaa tgtcgaaaca
     1201 gtgatagaaa aagaagatgt tgaaaccaat gcttcaaatg gtcagagagt tgatttatca
     1261 agtgaactag ataaactaaa gaaacttgaa aacgcaacag ttcacatgga gtttaagcca
     1321 gatgccaagg ccccagcatt ctataatctc ttttctgtgt caagtgctac taaaaaagat
     1381 gagtacttca ctatggcagt ttacaataat actgctactc tagaggggcg tggttcggat
     1441 gggaaacagt tttacaataa ttacaacgat gcacccttaa aagttaaacc aggtcagtgg
     1501 aattctgtga ctttcacagt tgaaaaaccg acagcagaac tacctaaagg ccgagtgcgc
     1561 ctctacgtaa acggggtatt atctcgaaca agtctgagat ctggcaattt cattaaagat
     1621 atgccagatg taacgcatgt gcaaatcgga gcaaccaagc gtgccaacaa tacggtttgg
     1681 gggtcaaatc tacagattcg gaatctcact gtgtataatc gtgctttaac accagaagag
     1741 gtacaaaaac gtagtcaact ttttaaacgc tcagatttag aaaaaaaact acctgaagga
     1801 gcggctttaa cagagaaaac ggacatattc gaaagcgggc gtaacggtaa cccaaataaa
     1861 gatggaatca agagttatcg tattccagca cttctcaaga cagataaagg aactttgatc
     1921 gcaggtgcag atgaacgccg tctccattcg agtgactggg gtgatatcgg tatggtcatc
     1981 agacgtagtg aagataatgg taaaacttgg ggtgaccgag taaccattac caacttacgt
     2041 gacaatccaa aagcttctga cccatcgatc ggttcaccag tgaatatcga tatggtgttg
     2101 gttcaagatc ctgaaaccaa acgaatcttt tctatctatg acatgttccc agaagggaag
     2161 ggaatctttg gaatgtcttc acaaaaagaa gaagcctaca aaaaaatcga tggaaaaacc
     2221 tatcaaatcc tctaccgtga aggagaaaag ggagcttata ccattcgaga aaatggtact
     2281 gtctatacac cagatggtaa ggcgacagac tatcgcgttg ttgtagatcc tgttaaacca
     2341 gcctatagcg acaagggtga tctatacaag ggtaaccaat tactaggcaa tatctacttc
     2401 acaacaaaca aaacttctcc atttagaatt gccaaggata gctatctatg gatgtcctac
     2461 agtgatgacg acgggaagac atggtcagcg cctcaagata ttactccgat ggtcaaagcc
     2521 gattggatga aattcttggg tgtaggtcct ggaacaggaa ttgtacttcg gaatgggcct
     2581 cacaagggac ggattttgat accggtttat acgactaata atgtatctca cttaaatggc
     2641 tcgcaatctt ctcgtgtcat ctattcagat gatcatggaa aaacttggca tgctggagaa
     2701 gcggtcaacg ataaccgtca ggtagacggt caaaagatcc actcttctac gatgaacaat
     2761 aaacgtgcgc aaaatacaga atcaacggtg gtacaactaa acaatggaga tgttaaactc
     2821 tttatgcgtg gtttgactgg agatcttcag gttgctacaa gtaaagacgg aggagtgact
     2881 tgggagaagg atatcaaacg ttatccacag gttaaagatg tctatgttca aatgtctgct
     2941 atccatacga tgcacgaagg aaaagaatac atcatcctca gtaatgcagg tggaccgaaa
     3001 cgtgaaaatg ggatggtcca cttggcacgt gtcgaagaaa atggtgagtt gacttggctc
     3061 aaacacaatc caattcaaaa aggagagttt gcctataatt cgctccaaga attaggaaat
     3121 ggggagtatg gcatcttgta tgaacatact gaaaaaggac aaaatgccta taccctatca
     3181 tttagaaaat ttaattggga ctttttgagc aaagatctga tttctcctac cgaagcgaaa
     3241 gtgaagcgaa ctagagagat gggcaaagga gagatgggca aaggagttat tggcttggag
     3301 ttcgactcag aagtattggt caacaaggat ccaacccttc aattggcaaa tggtaaaaca
     3361 gcgactttcc taacccagta tgatagcaag accttgttat ttgcagtaga taaggaagat
     3421 atcggacagg aaattattgg tatagctaaa ggaagcatcg aaagtatgca taatcttcct
     3481 gtaaatctag caggtgccag agttcctggc ggagtaaatg gtagcaaagc agcggtgcat
     3541 gaagttccag aatttacagg gggagttaat ggtacagagc cagctgttca tgaaatcgca
     3601 gagtataagg gatctgattc gcttgtaact cttactacaa aagaagatta tacttacaaa
     3661 gctcctcttg ctcagcaggc acttcctgaa acaggaaaca aggagagtga cctcctagct
     3721 tcactaggac taacagcttt cttccttggt ctgtttacgc tagggaaaaa gagagaacaa
     3781 taagagaaga attctaaaca tttgattttg taaaaataga aggagatagc aggttttcaa
     3841 gcctgctatc tttttttgat gacattcaag ctgatacgaa atcataagag gtctgaaact
     3901 actttcaggg tagtctgttc tataaaatat agtagattga aataagatgt gaacaactct
     3961 accaggaaag tcaaattaat ttatagaatt attttagcag tcaaggtgta ctgttataga
     4021 ttcaatatat tatgcgacta ttaaccttgt cttctcctaa aattgacttt cttgttttct
     4081 tatcttgtcc actcgaaaca agtattgtaa gaatttgatt atttttgaaa gtacttttaa
     4141 tatatttgat atagttaaaa aaatttaaaa ctaaattcca aattagaaaa agacttgaaa
     4201 tactaaaaaa aaaaaagtat actctaattg aaaacggtaa caaaactaat ttagagaatg
     4261 aaatatagag tatttctctc ttaaaagttt ttggtgaaac gagatgtaga aaggagattt
     4321 agccaaagag tctattagtg ctagaataat agattagaat tattttagaa aaacgaagtg
     4381 agcagcttat aaattcaagt ccccaaatag attcatacta gtatcttttg caaaaaataa
     4441 agggcgactt ccttcatgaa tatcaatttc atctataagg aaggtagcta attgaactaa
     4501 cttatttatt ctgtttgtcg ctagaaaaat cagacctcct tgtgaagatt gaggagatac
     4561 ttaatgaaaa tcaaagaaga aactagcaag ctagtagcag attgcccaaa acaccgcttt
     4621 gaggttgtag ataagactga cctatataat ccaaggtgaa gcgactgtgg tttgaagaga
     4681 ttttcaaaga gtataggcta gagagtagtg tttttatgtc cttctagtag aaaatgctag
     4741 acagaagaat ggggaacttg gataggaaaa atagattgag aaaggaggtt agaagagatg
     4801 attattacaa aaattagccg tttaggaact tatgtgggag tcaatccaca ttttgcaaca
     4861 ttaatagatt ttctagaaaa aacaggacta gaaaatttaa cagaaggttc gattgctatc
     4921 gatggtaatc gattgtttgg gaattgcttt acttatctag cagatggtca agcaggggct
     4981 ttctttgaaa cacaccaaaa atatttggat attcatttag ttttggaaaa cgaagaagcc
     5041 atggctgtta catcgccgga aaatgtaagc gttacccaag aatatgatga agagaaagat
     5101 attgaattat acacagggaa agtggaacag ttggttcagt tgagagctgg cgaatgcctc
     5161 atcacttttc cagaagattt acatcaaccc aaggttcgta taaatgatga acctgtgaaa
     5221 aaagttgtct ttaaagttgc gatttcttaa tgtagaaaga gaagaacgat gaaaaaaatg
     5281 agaaagtttt tatgtctagc tggaattgcg ctagcggctg ttgccttggt agcttgttca
     5341 ggaaaaaaag aagctacaac tagtactgaa ccaccaacag aattatctgg tgagattaca
     5401 atgtggcact cctttactca aggaccccgt ttagaaagta ttcaaaaatc agcagatgct
     5461 ttcatgcaaa agcatccaaa aacgaaaatc aagattgaaa cattttcttg gaatgacttc
     5521 tatactaaat ggactacagg tttagcaaat ggaaatgtgc cagatatcag tacagctctt
     5581 cctaaccaag taatggaaat ggtcaactca gatgctttgg ttccgctaaa tgattctatc
     5641 aagcgtattg gacaagataa atttaacgaa actgccttaa atgaagcaaa aatcggagat
     5701 gattactact ctgttcctct ttattcacat gcacaagtca tgtgggttag aacagatttg
     5761 ttaaaagaac ataatattga ggttcctaaa acttgggatc aactctatga agcttctaaa
     5821 aaattgaaag aagctggagt ttatggcttg tctgttccgt ttggaacaaa tgacttaatg
     5881 gcaacacgtt tcttgaactt ctacgtacgt agtggtggag gaagcctctt aacaaaagat
     5941 cttaaagcag acttgacaag ccaacttgct caagatggta ttaaatactg ggttaaattg
     6001 tataaagaaa tctcacctca agattctttg aactttaatg tccttcaaca agctaccttg
     6061 ttctatcaag gaaaaacagc atttgacttt aactctggct tccatatcgg aggaattaat
     6121 gccaacagtc ctcaattgat tgattcgatt gatgcttatc ctattccaaa aatcaaagag
     6181 tctgataaag accaaggaat tgaaacctca aacattccaa tggttgtttg gaaaaattca
     6241 aaacatccag aagttgctaa agcattctta gaagcacttt ataatgaaga agactacgtt
     6301 aaattccttg attcaactcc agtaggtatg ttgccaacta ttaaggggat tagcgattct
     6361 gcagcctata aagaaaatga aactcgtaag aaatttaaac atgctgaaga agtaattact
     6421 gaagctgtta aaaaaggtac tgctattggt tatgaaaatg gaccaagtgt acaagctggt
     6481 atgttgacta accaacacat tattgaacaa atgttccaag atatcattac aaatggaaca
     6541 gatcctatga aagcagcaaa agaagcagaa aaacaattaa acgatttatt tgaggctgtt
     6601 cagtagatgt aaaagactag aaaataggtg ggctagtgag ctgaaaagct ctagcccaat
     6661 cttgtaaaag aagggagaag gagaatggtt aaagaacgta atttaactcg ctggatattt
     6721 gttttgccag ctatgattat cgtaggatta ctctttgttt atccgttttt ctcgagtatt
     6781 ttttatagct ttaccaataa gcatttgatt atgcctaatt ataaatttgt tggtttggct
     6841 aactataaag ctgtgctatc agatcccaac ttctttaatg cgttctttaa ttcaattaag
     6901 tggaccgttt tctcattagt tggtcaagtt ttagtagggt ttgtattggc tttagctctt
     6961 cacagagtac gccacttcaa gaaattatat aggaccttat tgattgttcc ttgggcattt
     7021 cctaccatcg ttattgcctt ctcttggcag tggattctaa acggggttta tggctactta
     7081 cctaatctaa tcgtaaaatt aggtttaatg gaacatacac ctgcattttt gacagatagt
     7141 acatgggcat tcctatgttt ggtgtttatc aacatttggt ttggagcacc aatgattatg
     7201 gttaatgtgc tttcagcttt gcaaacagta ccagaagaac aatttgaggc tgctaagata
     7261 gatggtgctt caagttggca ggtgttcaag tttatcgtct ttccacatat taaagtggtt
     7321 gtaggacttc tagttgtttt gagaactgta tggatcttta ataactttga cattatctac
     7381 ctcattactg gtggtggacc agccaatgct acaacgacgc ttccaatttt tgcttacaac
     7441 ctgggttggg gaactaaatt gttgggtcgt gcttcagcag ttacagtatt gctctttatc
     7501 ttcttggtgg cgatttgctt tatctacttt gctatcatca gtaagtggga aaaggagggt
     7561 agaaaataat gaagaagaaa tccagtattt atttagatat tctctcacat gtacttttag
     7621 ttggtgcgac catcgttgca attttcccat tggtatggat tatcatatct tctgtcaaag
     7681 ggaaagggga attaactcag tatccaacac gattttggcc tgaacagttt acattagatt
     7741 atttcactca tgttatcaac gatttgcact tcattgataa cattcgaaac agtttaatca
     7801 ttgccttggc tacaaccctc attgcgatta ttatttctgc tatggcagcc tatggtattg
     7861 ttcgattctt tcctaaattg ggagcaatca tgtcgagact actcgtcatt acctacattt
     7921 tcccaccaat tttgttagca attccctatt caattgccat tgctaaagtt gggttaacaa
     7981 atagtttatt tggcttgatg atggtttatc tatcttttag tgttccatat gcagtttggc
     8041 tcttagttgg atttttccaa acagttccaa ttggaattga agaagcggct agaattgatg
     8101 gtgcaaataa atttgttacg ttttataaag ttgtgctacc gattgtagca ccaggtattg
     8161 tagcaacagc tatttataca tttatcaatg cttggaatga attcctgtat gccttgattt
     8221 tgattaacaa tacaggaaag atgacagtag cagtagccct tcgttcactt aatggttcag
     8281 aaatactaga ctggggagat atgatggcag cgtctgttat tgtagttctt ccatcaatta
     8341 ttttcttctc tatcatccaa aataagattg caagtggatt atcagaagga tctgtgaagt
     8401 agacgaaaga aggaaaaaaa tgaataaaag aggtctttat tcaaaactag gaatttctgt
     8461 tgtaggcatt agtcttttaa tgggagtccc cactttgatt catgcgaatg aattaaacta
     8521 tggtcaactg tccatatctc ctatttttca aggaggctca tatcaactga acaataagag
     8581 tatagatatc agccctttgt tattagataa attgtctgga gagagtcaga cagtagtaat
     8641 gaaatttaaa gcagatgaac caaactctct tcaagctttg tttggcctat ctaatagtaa
     8701 agcaggcttt aaaaataatt acttttcaat tttcatgaga gattctggtg agataggtgt
     8761 agaaataaga gacgcccaaa agggaataaa ttatttattt tccagaccag cttcattatg
     8821 gggaaaacat aaaggacagg cagttgaaaa tacactagta tttgtatctg attctaaaga
     8881 taaaacatac acaatgtatg ttaatggaat agaagtgttc tctgaaacag ttgatacatt
     8941 tttgccaatt tcaaatataa atggtataga taaggcaaca ctaggagctg ttaatcgtga
     9001 aggtaaggaa cattacctcg caaaaggaag tattggtgaa atcagtctat ttaacaaagc
     9061 aattagtgat caggaagttt caactattcc cttatcaaat ccatttcagt taattttcca
     9121 atcaggagat tctactcaag ctaactattt tagaataccg acactatata cattaagtag
     9181 tggaagagtt ctatcaagta ttgatgcacg ttatggtggg actcatgatt ctaaaagtaa
     9241 gattaatatt gccacttctt atagtgatga taatgggaaa acgtggagtg agccaatttt
     9301 tgctatgaag tttaatgact atgaggagca gttagtttac tggccacgag ataataaatt
     9361 aaagaatagt caaattagtg gaagtgcttc attcatagat tcatccattg ttgaagataa
     9421 aaaatctggg aaaacgatat tactagctga tgttatgcct gcgggtattg gaaataataa
     9481 tgcaaataaa gccgactcag gttttaaaga aataaatggt cattattatt taaaactaaa
     9541 gaagaatgga gataacgatt tccgttatac agttagagaa aatggtgtcg tttatgatga
     9601 aacaactaat aaacctacaa attatactat aaatgataag tatgaagttt tggagggagg
     9661 aaagtcttta acagtcgaac aatattcggt tgattttgat agtggctctt taagagaaaa
     9721 acataatgga aaacaggtcc ctatgaatgt tttctacaaa gattcgttat ttaaagtgac
     9781 tcctactaat tatatagcaa tgacaactag tcagaataga ggagagagtt gggaacaatt
     9841 taagttgttg cctccgttct taggagaaaa acataatgga acttacttat gtcccggaca
     9901 aggtttagca ttaaaatcaa gtaacagatt gatttttgca acatatacta gtggagaact
     9961 aacctatctc atttctgatg atagtggtca aacatggaag aaatcctcag cttcaattcc
    10021 gtttaaaaat gcaacagcag aagcacaaat ggttgaactg agagatggtg tgattagaac
    10081 attctttaga accactacag gtaagatagc ttatatgact agtagagatt ctggagaaac
    10141 atggtcggaa gtttcgtata ttgatggaat ccaacaaact tcatatggca cacaagtatc
    10201 tgcaattaaa tactctcaat taattgatgg aaaagaagca gtcattttga gtacaccaaa
    10261 ttctagaagt ggccgtaagg gaggtcaatt agttgtcggt ttagtcaata aagaagatga
    10321 tagtattgat tggaaatacc actatgatat tgatttgcct tcgtatggtt atgcctattc
    10381 tgcgattaca gaattgccaa atcatcacat aggtgtactg tttgaaaaat atgattcgtg
    10441 gtcgagaaat gaattgcatt taagcaatgt agttcagtat atagatttgg aaattaatga
    10501 tttaacaaaa taaaggagaa aaacatggtt aaatacggtg ttgttggagc agggtatttt
    10561 ggagctgaat tggctcgcta catgcaaaag aatgatggag cagagattac tcttctctat
    10621 gatccagata atgcagaggc gattgcagaa gaattgggag caaaagtagc aagttcctta
    10681 gatgagttgg tttctagcga tgaagtagat tgtgttatcg tcgcaactcc aaataatctt
    10741 cataaggaac cggttattaa ggctgcacag catggtaaaa atgttttctg tgaaaaacca
    10801 attgcgcttt cttatcaaga ttgtcgcgag atggtagatg cgtgtaaaga aaacaatgta
    10861 acctttatgg caggacatat tatgaatttc tttaatggtg ttcatcatgc aaaagaactc
    10921 attaatcaag gagttatcgg agacgttcta tattgtcata cagctcgtaa tggttgggaa
    10981 gaacaacaac cgtcagtatc atggaaaaaa attcgtgaaa aatcaggtgg tcacttgtat
    11041 caccacatcc atgaattgga ttgcgttcaa ttccttatgg ggggcatgcc tgaaactgta
    11101 accatgacag gtggaaatgt ggcccatgaa ggtgaacatt tcggtgatga agatgatatg
    11161 atttttgtca atatggaatt ttctaataag cgttttgctt tgttagaatg gggttcagct
    11221 tatcgttggg gtgaacatta tgtcttaatc caaggaagca aaggtgccat ccgcttagac
    11281 ttattcaact gtaaaggaac tcttaagcta gatgggcaag aaagctattt cttgattcac
    11341 gaatcgcaag aagaagatga tgatcggact cgtatctatc atagtacaga gatggatgga
    11401 gcaattgctt atggtaaacc aggtaaacgt actccattat ggctatcatc tgtcattgat
    11461 aaagaaatgc gctatctgca tgagattatg gaaggagctc cagtatcaga agaatttgca
    11521 aaacttttga caggtgaagc tgccctagaa gcaattgcta ctgcagatgc ttgtacccag
    11581 tctatgtttg aagatcgcaa agtaaaattg tcagaaattg taaaataaat tttggtattc
    11641 tcctatttat aggtcgactt gctcctctga aagtactttt agaggagctg tttgactttg
    11701 ctagtttttg aaactgaaat ctattatact acaaactatt gaaagcgttt taattttaag
    11761 gtataataat ctcatagaaa taaagaaaag gaggaaagag gatgccacag attagcaaag
    11821 aagccttgat tgagcaaatc aaagatggaa tcatcgtttc ttgtcaggct cttcctcatg
    11881 aaccgcttta tacagaagcg ggaggggtga ttcccttgct ggtcaaagcg gctgagcaag
    11941 gtggagcagt cggtatccga gcaaacagtg ttcgcgatat caaggaaatt aaggaagtca
    12001 ctaaacttcc aatcattggg attatcaaac gtgattatcc acctcaggaa cccttcatca
    12061 cggctactat gaaagaagtt gatgaattgg cagaactgga catcgaggtg attgctctgg
    12121 attgtaccaa gcgtgaacgc tacgatggtt tggaaattca agagttcatt cgtcaggtta
    12181 aggagaaata tcctaatcag cttttgatgg ctgatactag tatcttcgaa gaagggctag
    12241 cagctgtaga agcaggaatt gactttgtcg gaacaacctt atcaggctac acatcctaca
    12301 gtccaaaagt agacggtcca gattttgaat tgattaagaa actctgtgat gccggtgtag
    12361 atgtcattgc agaaggaaaa attcatacac cagaacaagc caaacaaatc cttgaatatg
    12421 gagtgcgagg catcgttgtt ggtggcgcca ttactagacc aaaagagatt acagaacgct
    12481 tcgttgctag tcttaaataa gatgtgaggg ggagttttat gtttaaagtt ttacaaaaag
    12541 ttggaaaagc ttttatgtta cctatagcta tacttcctgc agcaggtcta cttttgggga
    12601 ttggtggtgc actttcaaac ccaaccacga tagcaactta tccaatacta gacaatagta
    12661 tttttcaatc aatattccaa gtaatgagct ctgcaggaga ggttgtattc agtaatttgt
    12721 cactacttct ctgtgtggga ttatgtattg gcttagcgaa acgagataaa ggaaccgctg
    12781 cgttagcagg agtaactggt tacttagtta tgactgcaac gatcaaagct ttggtaaaac
    12841 tttttatggc agaaggatct gcaattgata ctggagttat tggagcatta gctgtcggaa
    12901 tagttgccgt atatttgcac aaccgatata acaatattca attaccttcc gctttaggat
    12961 tctttggagg ttcacgcttc gttcctattg ttacatcgtt ctcttctatc ttgattggct
    13021 ttgtcttctt tgttatttgg ccacctttcc aacaacttct tgtttctaca ggtggatata
    13081 tttctcaggc gggtccaatt ggaacttttc tatatggatt tttaatgaga ctttctggag
    13141 cagtaggctt acatcatata atttacccta tgttttggta tactgaactt ggtggtgttg
    13201 aaactgttgc aggacaaaca gtggttggag ctcaaaaaat attttttgct caattagccg
    13261 atttggccca ttctggatta tttacagaag gaacaaggtt ttttgcaggt cgtttctcaa
    13321 caatgatgtt cggtttaccg gctgcctgtt tagcgatgta ccatagtgtt cctaaaaatc
    13381 gtcgtaaaaa atacgcgggt ttgttttttg gagttgcttt aacatctttt attaccggta
    13441 ttacagaacc aattgaattt atgtttctat tcgtcagtcc ggttctatat gttgttcacg
    13501 cattccttga tggtgttagc ttctttattg cagacgtctt aaatatttca ataggaaaca
    13561 cattttcagg aggtgtaatc gatttcactt tatttggaat tttgcagggg aacgctaaga
    13621 cgaattgggt tcttcagatt ccatttggac ttatttggag tgttttgtat tatattattt
    13681 ttagatggtt cattactcaa ttcaacgttc taacgccagg gcgaggagaa gaagtagatt
    13741 ctaaagaaat ttctgaatcc gcagattcaa cttcaaatac tgcagattat ttaaaacagg
    13801 atagcctaca aattatcaga gccttgggtg gatcaaataa tatagaagat gtagatgctt
    13861 gtgtgacacg tttacgtgta gctgtaaaag aagttaatca agttgataaa gcacttttaa
    13921 aacaaattgg tgcagttgat gtcttagaag tgaagggtgg cattcaagca atctatggag
    13981 caaaagcaat cttatataaa aatagtatta atgaaatttt aggtgtagat gattaagtac
    14041 ttactgactt aataaaaaac agaggagagt gatggatgag taggatgaaa tgaaatcgca
    14101 tacaagaaat aaagaactca ttatccaagt tggatacgct tattacatag gagaatacaa
    14161 atgaaattta gaaaattagc ttgtacagta cttgcgggtg ctgcggttct tggtcttgct
    14221 gcttgtggca attctggcgg aagtaaagat gctgccaaat caggtggtga cggtgccaaa
    14281 acagaaatca cttggtgggc attcccagta tttacccaag aaaaaactgg tgacggtgtt
    14341 ggaacttatg aaaaatcaat catcgaagcg tttgaaaaag caaacccaga tataaaagtg
    14401 aaattggaaa ccatcgactt caagtcaggt cctgaaaaaa tcacaacagc catcgaagca
    14461 ggaacagctc cagacgtact ctttgatgca ccaggacgta tcatccaata cggtaaaaac
    14521 ggtaaattgg ctgagttgaa tgacctcttc acagatgaat ttgttaaaga tgtcaacaat
    14581 gaaaacatcg tacaagcaag taaagctgga gacaaggctt atatgtatcc gattagttct
    14641 gccccattct acatggcaat gaacaagaaa atgttagaag atgctggagt agcaaacctt
    14701 gtaaaagaag gttggacaac tgatgatttt gaaaaagtat tgaaagcact taaagacaag
    14761 ggttacacac caggttcatt gttcagttct ggtcaagggg gagaccaagg aacacgtgcc
    14821 tttatctcta acctttatag cggttctgta acagatgaaa aagttagcaa atatacaact
    14881 gatgatccta aattcgtcaa aggtcttgaa aaagcaacta gctggattaa agacaatttg
    14941 atcaataatg gttcacaatt tgacggtggg gcagatatcc aaaactttgc caatggtcaa
    15001 acatcttaca caatcctttg ggcaccagct caaaatggta tccaagctaa acttttagaa
    15061 gcaagtaagg tagaagtggt agaagtacca ttcccatcag acgaaggtaa gccagctctt
    15121 gaataccttg taaacgggtt tgcagtattc aacaataaag acgacaagaa agtcgctgca
    15181 tctaagaaat tcatccagtt tatcgcagat gacaaggagt ggggacctaa agacgtagtt
    15241 cgtacaggtg ctttcccagt ccgtacttca tttggaaaac tttatgaaga caaacgcatg
    15301 gaaacaatca gcggctggac tcaatactac tcaccatact acaacactat tgatggattt
    15361 gctgaaatga gaacactttg gttcccaatg ttgcaatctg tatcaaatgg tgacgaaaaa
    15421 ccagcagatg ctttgaaagc cttcactgaa aaagcgaacg aaacaatcaa aaaagctatg
    15481 aaacaatagt ccttagttat tctataaaaa gtagtttttt aaagaaccta agagtgtata
    15541 cccccttttc cctctacaca gatagtgtaa gaaaaggggg cttttgttta aaatgtaaga
    15601 aactgtcacg aaattaaaat gaagttctta cataagcgaa tcataaaaaa tttcattttg
    15661 attttaaaac agttcaagaa agtcaaaaaa ttattctatt tgaaagagag gtgccgactg
    15721 tgaaagtcaa taaaatccgt atgcgggaaa cagtgatttc ctacgctttc ctagcaccag
    15781 tattattctt ctttgtcatc tttgtgttgg ctccgatggt gatgggcttc attacaagtt
    15841 tctttaacta ctcaatgact aaatttgagt ttgtaggctt ggataactat atccgtatgt
    15901 ttaaagatcc tgtctttaca aaatctctga ttaacacagt tattttggtt attggatctg
    15961 taccagttgt tgttctattc tcactctttg tagcatctca gacctatcat caaaatgtca
    16021 ttgccagatc cttctaccgt ttcgtcttct tccttcctgt tgtaacgggt agtgttgccg
    16081 tgacagttgt ttggaaatgg atttatgacc cactatcagg gattctaaac tttgtcctta
    16141 agtccagcca catcatcagc caaaacattt cttggttggg agataaaaac tgggcattga
    16201 tggcgattat gattattctc ttgaccactt cagttggtca gcccatcatc ctttatatcg
    16261 ctgccatggg gaatattgac aattcactgg ttgaagcggc gcgtgttgat ggtgcaactg
    16321 agtttcaagt tttttggaag attaaatggc caagccttct tccaacaact ctttatattg
    16381 caatcatcac aacaattaac tcattccagt gttttgcctt gattcagctt ttgacatctg
    16441 gtggtccaaa ctactcaaca agtaccttga tgtactacct ttacgaaaaa gccttccaat
    16501 tgacagaata cggctatgcc aacacaattg gtgtcttctt ggcaatcatg attgctatcg
    16561 taagctttgt tcaatttaaa gtacttggaa acgacgtaga atactaaaga aaggagacag
    16621 ctatgcaatc tacagaaaaa aaaccattaa cagcctttac tgttatttca acaatcattt
    16681 tgctcttgtt gactgtgctg ttcatctttc cattctactg gattttgaca ggggcattca
    16741 aatcacaacc tgatacaatt gttattcctc ctcagtggtt ccctaaaatg ccaaccatgg
    16801 aaaacttcca acaactcatg gtgcagaacc ctgccttgca atggatgtgg aactcagtat
    16861 ttatctcatt ggtaaccatg ttcttagtct gtgcaacctc atctctagca ggttatgtat
    16921 tggctaaaaa acgtttctat ggtcaacgca ttctatttgc tatctttatc gctgctatgg
    16981 cgcttccaaa acaagttgtc cttgtaccat tggtacgtat cgtcaacttc atgggaatcc
    17041 acgatactct ctgggcagtt atcttgcctt tgattggatg gccattcggt gtcttcctca
    17101 tgaaacagtt cagtgaaaat atccctacag agttgcttga atcagctaaa atcgacggtt
    17161 gtggtgagat tcgtaccttc tggagtgtag ccttcccgat tgtgaaacca gggtttgcag
    17221 cccttgcaat ctttaccttc atcaatactt ggaatgacta cttcatgcaa ttggtaatgt
    17281 tgacttcacg taacaatttg accatctcac ttggggttgc gaccatgcag gctgaaatgg
    17341 caaccaacta tggtttgatt atggcaggag ctgcccttgc tgctgttcca atcgtcacag
    17401 tcttcctagt cttccaaaaa tccttcacac agggtattac tatgggagcg gtcaaaggat
    17461 aatactctga gaaaatctct tcaaactacg tcagcgtcac cttgccatac ttaagtattg
    17521 cctgcggtta gcttcctagt ttgttcttca attttcattg agtataggaa aatcaatcta
    17581 tcaagataca gaagtatatt ttatagattt agagaatata gaggttataa gtgtctacaa
    17641 aatggagggt atgcagttac tttatgaagt tttgtcagac acttataaac ttaagaatgg
    17701 ttttagttaa ctatcagaaa cgaaggaaag agtatgattt ttgacgattt gaaaaacatc
    17761 accttttaca aagggattca tcccaattta gacaaggcta tcgactatct ctaccaacat
    17821 cgtaaagatt cattcgaatt aggaaagtat gagattgatg gagataaagt ctttctagtt
    17881 gttcaggaaa atgtcctcaa tcaagttgag aataatcgat ttgaacacca taagaactat
    17941 gcagatttgc atttgctgat agaagggcat gaatattcga gctacggttc acgtatcaaa
    18001 gacgaggcag tagcattcga cgaagcgagt gacattggct ttgttcattg tcatgaacac
    18061 tacccactct tgttgggtta tcacaatttt gcgattttct tcccaggtga gccacatcag
    18121 ccaaatggtt atgcaggcat ggaagaaaag gttcgaaaat atctctttaa aattttgatt
    18181 gattaaaaat aggatgaatt gtttttttgt aaagctttga taatactcta ccatgaaatt
    18241 gatctttgtg aggtagagaa atgagaataa aatatttaaa aattggtatc ttctaagtat
    18301 gctgcaagag ctagtttctt agatggacag gggattacag ttgatgagat ggcttggata
    18361 attaggggca ttgtgactgc attgattggt agatacataa aattaggtac ttatgcagtt
    18421 aagtatggta ttagtatggc gcgctcgatc ttaagtaggg cagctgcaac tgcggcagca
    18481 agagtaggat tattgactaa gatttttgga tggattttac gaatagctgt gctcgcagct
    18541 gatgtatatg gtaattttgc caacaatatt gctgtagctt gggatgcaca tgataaaatc
    18601 cctaacaatg gtcgtataaa cttttaaaat gcgagaatga aagcactttg tattttttta
    18661 ttgtatatgt tagcttggac agtgcttgca atgataatcc gtggagggct agatggattt
    18721 gatgaacaca cttggcgtac tattttaatt gcttcgctgt ccgggatata tatagattat
    18781 atgtccatag ataaaagtag aaaaaagtcc aaaagaaaaa agaaaaaata gatttgttca
    18841 tggtagggac ttatgaaagc tttactgaca aaaaagaaaa cagtttacaa agaaaaatga
    18901 tggaggagca aacatggcac aaaaaggagt aagccttatc aaggcagcat ttgatacaga
    18961 taactttctc atgcgtttta gtgagaaagt cttggacatc gtgacagcca atcttctttt
    19021 tgtcgtctct tgtttgccca tcgtgacgat tggagtggct aaaatcagcc tctacgagac
    19081 catgttcgaa gttaagaaga gcagacgggt gcctgttttt aaaatctatc taagatcttt
    19141 caagcaaaat ctgaaactag gtcttcagct gggtttaatg gagttaggaa ttgtgtttct
    19201 taccctttca gatctctatc ttttctgggg tcaaacagct ctgcccttcc aattgctgaa
    19261 agccatttgt ctaggcattc tgattttcct tacgattgtg atgttggcta gctatccaat
    19321 cgcggcacgt tatgatttaa cttggaaaga agtgttacaa aaagggctta tcttggctag
    19381 ttttaacttt ccttggttct tcctcatgtt agccattctt gtcctcattg tgatggttct
    19441 ttatctgtcc gccttcagtc tactcttagg tggctcagtc ttcctacttt ttgggtttgg
    19501 actattggtc tttatccaga ctggattgat ggagaaaatt ttcgcaaaat accaatagga
    19561 gccttatttc tgaaactact ttcaaaggct ccaaacgcta ttctataagc gagaaactaa
    19621 aatctaagtg tctataagat attgaaagcc ttttcggtaa agtgtatact aaagaagtaa
    19681 agaaaaatct gctttagcag aaaataaaaa taataggaga aaatctatgt cagatttgaa
    19741 aaaatacgaa ggtgtcattc cagccttcta cgcatgttat gatgagcaag gagaagtaag
    19801 tccagagcgt acgcgtgcct tggttcaata cttcattgat aaaggtgttc aaggtcttta
    19861 tgtcaatggt tcttctggtg aatgtatcta ccaaagcgtt gaagatcgca agttgatttt
    19921 ggaagaagtc atggcggtag ccaaaggtaa attgaccatt attgcccatg ttgcttgcaa
    19981 taatactaaa gatagtatgg aacttgctcg ccatgctgaa agcttgggag tagatgctat
    20041 tgcaacgatt ccaccaattt acttccgctt gccagaatac tcagttgcca aatactggaa
    20101 cgatattagt tctgcagctc caaacacaga ctacgtgatt tacaacattc ctcaattggc
    20161 aggggttgct ttgacttcaa gcctttacac agaaatgttg aaaaatcctc gtgttatcgg
    20221 tgttaagaac tcttcaatgc cagttcaaga tatccaaacc tttgtcagcc ttggcgggga
    20281 agaccatatt gtctttaacg gtccagatga acagttccta ggtggccgcc tcatgggtgc
    20341 taaggctggt atcggtggta cttatggtgc tatgccagaa ctcttcttga aactcaatca
    20401 gttgattgcg gataaagatt tagaaacagc gcgtgaattg cagtatgcta tcaacgcaat
    20461 cattggtaaa ctcactgctg cacatggaaa tatgtacggt gtcatcaaag aagttttgaa
    20521 gatcaatgaa ggcttgaata ttggttctgt tcgttcacca ttgacaccag tgactgaaga
    20581 cgatcgtcca gttgtagaag cggctgcagc cttgattcgt gaaaccaagg agcgcttcct
    20641 ctaatctaaa aggaggtatt tatgacatac tacgttgcaa ttgatatcgg tggaactaac
    20701 atcaagtatg gtttggttga tcaagagggg caacttcttg aatcacatga aatgccaaca
    20761 gaggcgcata agggtggacc tcatatctta caaaagacca aagatatcgt agctagttat
    20821 ttagaaaaag gcccagtagc aggtgttgcc atatcttctg ctgggatggt ggatccggat
    20881 aagggtgaga ttttctatgc tgggccgcaa atccctaact acgcaggcac ccagttcaaa
    20941 aaggaaatcg aagaaagctt tactattcct tgtgagattg aaaatgatgt caactgtgca
    21001 ggtcttgctg aggcagtatc tggttcaggc aagggagcaa gtgtgacact ttgcttgacc
    21061 attggaaccg gtatcggtgg ttgcttgatt atggatagga aagtcttcca tggttttagt
    21121 aattcagcct gtgaagtcgg gtatatgcat atgcaggatg gagcttttca ggatctcgct
    21181 tctacaacag ctttagtgaa atatgtagct gaagcccatg gagaagatgt tgatcagtgg
    21241 aatggccgac gtatctttaa ggaagctact gaaggaaaca aaatctgcat ggaaggtatt
    21301 gaccgtatgg ttgactatct aggaaaaggt ctggcaaata tttgctacgt tgccaatcca
    21361 gaagtggtca ttcttggtgg tggtatcatg gggcaagagg ctatcctcaa acctaagatc
    21421 cgcacagcct tgaaagaggc tttggtacca agtttagcag aaaaaacacg attagaattt
    21481 gcccatcacc aaaatacagc agggatgttg ggtgcatatt atcattttaa gacaaaacaa
    21541 tcctagtttg gctcagccaa actaggattt tcttacacgt ttttgtctac gatagccgtt
    21601 gagtttttta ttttcccagt agctattaaa gattttttcc ttgctttcgc gattgatttc
    21661 caaaaagtag gcataaatca aatcgataaa gaagagcata ggaagttgag cggatattcg
    21721 ttggatatag gagggttggc tgtgggtggc tacaagaaca gtctctgtat aggtctggct
    21781 atttttattg ggaacacttg taaagagtac agtctttgcc cccatctcct tggcatctaa
    21841 tagactatct aaaatagaag gagttgagcc tgaaagtgag aagcccagta ctagacaatt
    21901 ttcatccatg atgctggttg tccaggcaaa gccgtcttgg tctgtcaaag cttcgcagac
    21961 cacacctaat cgcataaaac gtaatttcat ttcacgggcg acgaggccag aactccctgt
    22021 tccaaagaag tagatacgct cagcatcttc gattagctgg gcaattcgtt ctagttggat
    22081 ttcgtcaatc aagtcttgtg tttgttccct catattgcta taacttctga ggactcgttt
    22141 ggtcagtgga ctgtgcttgg aaacttggtt ggcttgattt tctgcctgat gttggtattg
    22201 gaaaataaat tctcggtagc cagtaaagcc acacttttta gcaaagcggg tcaaagcagc
    22261 ttgagaaata tgtaattttt gggtgacttg ttgagaagat aaatcatctg taatcgtttc
    22321 agcttgcaaa aaatagcgag cgatttcttg ctctaggtct gtcatttctt caaaatgtga
    22381 atcaatgata gttgcgatat ctggtttgtc catagggaaa gctcctttac atgagtcata
    22441 ctggaagact agatcaggga atagtcacac ttcattataa cacataatat aaggatagat
    22501 aaataaaaac gcatctctgt tttaaaaacg aaaaaatcga aaaagcttct ctcttttcca
    22561 taattttcta ctcaaattgt ggtacaatta agagtaagat tttaagttag aaatgagact
    22621 gatttgtatg agaaaattta acagccattc gattccgatt cggcttaatt tattgttttc
    22681 aatcgtcatt ttactcttta tgaccattat tggtcgtttg ttgtatatgc aggttttgaa
    22741 caaggatttt tacgaaaaaa agctagcctc agctagtcag accaagatta caagcagttc
    22801 agcccgtggg gaaatttatg atgctagtgg aaaacctttg gtagaaaata cgttaaagca
    22861 ggttgtttcc tttacgcgta gcaataaaat gacggctaca gacttaaaag aaacagctaa
    22921 aaagttactg acttatgtga gcatcagttc tccaaatttg acagaacgcc agctggcgga
    22981 ttactatttg gctgatcctg aaatctataa aaaaatagtg gaagctctcc caagcgagaa
    23041 acgcttggat tcagatggca atcgtctatc cgaatcagaa ctgtataaca atgcggtcga
    23101 tagtgtacaa acgagtcaac taaactatac agaggatgaa aagaaagaaa tctatctttt
    23161 tagtcagtta aatgctgttg gaaactttgc gacaggaacc attgctacag atcctctaaa
    23221 tgattctcag gtggctgtta ttgcctctat ttcaaaggag atgcctggca ttagtatttc
    23281 tacttcttgg gatagaaagg ttttggaaac ttccctttct tctatagttg ggagtgtatc
    23341 cagtgaaaaa gctggtctcc cagcggaaga agcagaagcc tatcttaaaa aaggctattc
    23401 tctaaatgat cgtgtaggaa cctcctattt ggaaaagcaa tatgaagaga ccttacaagg
    23461 aaaacgctcg gtaaaagaaa tccatctgga taaatatggc aatatggaaa gcgtggatac
    23521 aattgaggaa ggtagtaagg gaaacaatat caaactgacc attgatttgg ctttccaaga
    23581 tagcgtggat gctttactga aaagttattt caattctgag ctagaaaatg gtggagccaa
    23641 gtattctgaa ggtgtctatg cagtcgccct taacccaaaa acaggtgcgg ttttgtctat
    23701 gtcagggatt aaacatgact tgaaaacggg agagttgacg cctgattcct tgggaacggt
    23761 aaccaatgtc tttgttccag gttcggttgt caaggcggcg accatcagct caggttggga
    23821 aaatggagtc ttgtcaggaa accagacctt gacagaccag tccattgtct tccaaggttc
    23881 agctcccatc aattcttggt atactcaggc ttacggttca ttccctatca cagcggtcca
    23941 agctctggag tattcatcaa atacctatat ggtccaaaca gccttaggtc ttatggggca
    24001 aacctatcaa cccaatatgt ttgtcggcac cagcaatcta gagtctgcta tggagaaact
    24061 gcgttcaacc tttggcgaat atggcttggg tactgcgaca ggaattgacc taccagatga
    24121 atctactgga tttgttccca aagagtatag ctttgctaat tacattacta atgcctttgg
    24181 gcagtttgat aactatacgc cgatgcagtt ggctcagtat gtagcaacta ttgcaaataa
    24241 tggtgttcgt gtggctcctc gtattgttga aggcatttat ggtaataatg ataagggagg
    24301 actgggtgac ttgattcagc aactgcaacc gacagagatg aataaggtca atatatccga
    24361 ctccgatatg agcatcttgc accaaggttt ttatcaggtt gcccatggta ctagtggatt
    24421 gacaactgga cgtgcctttt caaatggtgc cttggtatcc attagcggaa aaacaggtac
    24481 agccgaaagc tatgtggcag atggtcagca agcaaccaat accaatgcgg tggcctatgc
    24541 cccatctgat aatccccaaa tcgctgtcgc agtggtcttt cctcataata ccaatctaac
    24601 aaatggtgta ggaccttcca ttgcgcgtga cattatcaat ctgtatcaaa aataccatcc
    24661 aatgaactag aaaggaaatt atgctttatc caacacctat tgccaagttg attgacagtt
    24721 attctaagtt accaggtatc gggattaaga cggctacgcg tctggccttt tatacgattg
    24781 ggatgtctgc tgatgatgtc aatgaatttg caaaaaatct cctttctgct aagagagaat
    24841 tgacatattg ttctatttgt ggacgtttga cagacgacga tccttgttct atctgtactg
    24901 attcgactcg tgaccagaca acaattttag ttcttgagga tagtagagat gtggcagcca
    24961 tggaaaatat ccaagaatac catggactct atcatgtcct gcatggcctc atttctccta
    25021 tgaatggtat cagtccggac gatatcaatc ttaagagcct tatgactcgt cttatggata
    25081 gtgaggtttc agaagtgatt gtggcgacta atgctacagc ggatggtgaa gcgacctcca
    25141 tgtatctttc acgtttgctc aagccggctg gtatcaaggt tacgcgtcta gcacgaggtc
    25201 tcgctgtggg agcggacatt gagtatgcgg acgaagtgac actcttacga gcgattgaaa
    25261 atcggacaga gttgtaagtg taggcaaatt tacgaactcc attcatttat aaaaaatcaa
    25321 agaggctgaa aatcgttcct atcggcctct ttttgtatag tgtgatgagt agaatcaggt
    25381 tcaagtttta aaaaaccaag caaatatgat atactaaaga gcgagtattc tagtagaatt
    25441 aggacaaata atatgaaaca aacgattatt cttttatatg gtggacggag tgcggaacgc
    25501 gaagtctctg tcctttcagc tgagagtgtc atgcgtgcgg tcaattacga ccgtttcaca
    25561 gtcaagactt tctttatcag tcagtcaggt gactctatca aaacgcagga atttagtcat
    25621 gctccggggc aagaagaccg tctcatgacc aatgaaacca ttgattggga taagaaagtt
    25681 gcaccaagtg ctatctacga agaaggtgca gtggtctttc cagtccttca cgggccaatg
    25741 ggagaagatg gctctgttca aggattcttg gaagttttga aaatgcctta cgttggttgc
    25801 aacattttgt catcaagtct tgccatggat aaaatcacga ctaagcgtgt tctggaatct
    25861 gctggtattg cccaagttcc ttatgtggct atcgttgaag gcgatgatgt gactgctaaa
    25921 atagctgaag tggaagaaaa attggcttat ccagtcttca ctaagccgtc aaacatgggg
    25981 tctagtgtcg gtatttctaa gtctgaaaac caagaagaac tccgtcaagc cttaaaactt
    26041 gccttccgat atgacagccg tgtcttggtt gagcaaggag tgaatgcccg tgaaattgag
    26101 gttggcctct tgggtaacta cgatgtcaag agcacgctac ctggagaagt tgtcaaggac
    26161 gttgcctttt atgactacga tgccaagtat attgataaca aggttactat ggatattcct
    26221 gccaaaatca gtgatgatgt ggtggctgtc atgcgtcaaa atgcagaaac agccttccgt
    26281 gccattggtg gccttggtct atctcgttgc gatttcttct atacagataa gggagagatt
    26341 tttctcaacg agctcaatac catgccaggt ttcacccagt ggtctatgta cccactactt
    26401 tgggaaaata tggggatcag ctacccagaa ctaatcgagc gtttggttga ccttgccaag
    26461 gaaagttttg acaagcgcga agcgcatttg atataaaatg aaagagaggg tagaagccag
    26521 aaccatcact gcaaggtgac tagagttctc ggacttcagc cctttttaaa ggagtagaaa
    26581 tgaaattaac aatccatgaa attgcccaag ttgttggagc caaaaatgat atcagtatct
    26641 ttgaggacac tcagttagaa aaagctgagt ttgatagtcg tttgattgga actggagatt
    26701 tatttgtgcc acttaaaggt gcgcgtgatg gccatgactt tattgaaaca gcctttgaaa
    26761 atggtgcagc agtaaccttg tctgagaaag aggtctcaaa tcatccttac attttagtag
    26821 atgatgtttt gacagccttt caatccctag catcctacta tcttgaaaaa acgactgttg
    26881 atgtctttgc tgttacaggt tcaaatggca agacaacgac taaggatatg ttggcgcatt
    26941 tactgtcaac aagatacaag aactacaaaa cacaaggcaa ttacaataat gagattggcc
    27001 ttccttacac agttcttcat atgcctgaag gaacagaaaa gttggttttg gagatgggac
    27061 aggatcactt gggagatatt catctcttgt ctgaattggc tcgtccaaaa acagccatcg
    27121 tgaccttggt tggagaagct catttggcct ttttcaaaga tcgttctgag attgctaaag
    27181 gaaaaatgca aattgcagac ggaatggctt caggttcctt gcttttagcg ccggctgacc
    27241 ctatcgtaga ggactatttg ccaattgata aaaaggtggt tcgttttggt caaggagcag
    27301 agctggaaat tactgacttg gttgagcgta aggacagtct gacctttaag gctaatttct
    27361 tggaacaagc ccttgatttg ccagtaactg gcaagtacaa tgcgacaaat gctatgattg
    27421 catcctatgt tgccttgcaa gaaggagttt cagaggagca aattcgtttg gcattccaac
    27481 atcttgaatt gacgcgtaac cgtaccgagt ggaagaaagc agccaatgga gcagatatcc
    27541 tgtcagatgt ttacaatgcc aatccaactg ctatgaaact gattttagag actttctctg
    27601 ccattccagc caatgaaggt ggcaagaaaa ttgcagtgtt ggcggatatg aaggagcttg
    27661 gtgaccagtc tgttcaactt cataatcaga tgattttgag cctctcccca gatgtgcttg
    27721 atatcgttat tttctatgga gaagatattg ctcagttggc tcaattggcc agtcaaatgt
    27781 ttccaatcgg ccacgtttac tacttcaaga aaacagaaga ccaggatcaa tttgaagacc
    27841 tagtcaagca ggttaaggaa agccttggag cctatgacca aatcttgcta aaaggttcta
    27901 actctatgaa tctagccaaa ttggtagaaa gtttagaaaa tgaatgcaag tgattttgct
    27961 aagtatcttc aaagaatgat tgctcttaca gatactggac taacctttac aaaggatccg
    28021 tttgaccgtg agcgctacga agacttgcga agtctgttat ctgaaatgtt gaatcaggta
    28081 tcagaccttg atgcagacga agtggcagaa ctcttgaaac cgacctccgc ttatgcgact
    28141 cctttaatgg acgtccgtgc ttggatagtt gaggatgaaa aaatttgtct ggttagggga
    28201 caaggagaga atgattgggc tttgccaggt ggctttggcg aggtcggtta ttcaccaact
    28261 gaaaatattc tcaaggaaat tgaagaagaa accggtttta aagccaaagt tgaacgacta
    28321 ctagctgttt ttgataccaa tcgtttccaa ctacagagca aacaatatgc aaagtttgtt
    28381 tttgaatgta agcttctcga tggacaattt caagaaaatc aagaaatcgc tgaccttcaa
    28441 ttttttgcaa ttgaccaact gccgaattta tctgaaaaac gcattaccaa ggagcaaata
    28501 gagcttcttt ggcaggttta tcaaggtcat agggggcaat atcttgacta agaagatgat
    28561 tatcgtattt ctaaatccat ttttaacaac tagcatggta taataatatg caggaaaatt
    28621 ttgaattatg aggaagacta gatgaattta tgggatattt tctttacgac tcaggcaacc
    28681 gagccgccca aatttgacct tttttggtat gttagcctat ttacgctctt agccttaacc
    28741 ttttatacag cccatcgcta tcgtgaaaat aaggtttacc aacgattttt ccaaatcttg
    28801 cagactgttc agttaatcct tctttatggt tggtactggg tcaatcatat gccactgtca
    28861 gaaagcctac ccttttacca ttgccgtatg gctatgtttg tggtactctt gcttcctggt
    28921 caatccaaat ataaacaata ctttgcatta ttgggaacat ttgggacatt agcagccttt
    28981 gtttatccag tgccagatgc ttaccctttt ccacatatca ccattctatc ctttatcttt
    29041 ggtcatttag cactcttggg gaactctcta gtttatctat tgagacagta taatgcgcga
    29101 ttgctggatg tgaagggaat ttttctcatg acctttgccc taaatgcctt gatttttgtg
    29161 gtcaatttgg tgacaggtgg cgattacgga tttttgacaa aaccgccatt ggttggggat
    29221 cacggtctag tagctaatta tttacttgtt tcaattgtgc tggtagctac tatcagtttg
    29281 actaagaaaa tcttagaatt ctttttagct caagaagcag aaaaaatgat tgcaaaggaa
    29341 gcttaacaca gagctttctt ttttgctctt agagagtttt tacaagcagc ttataaaata
    29401 agaatttctg aatagacaaa ctcaaaaaat ggctgggaaa tttaggaaaa aagcaagcac
    29461 gattaaattt tttgtgttat aatattttgt gaatagctat gcctatgttt agctatggaa
    29521 taatacgaag tgcgaaactt ggaagataga gaggaagcga tgtaatggct agagaaggct
    29581 tttttacagg tctagatatt ggaacaagct ctgtcaaggt gcttgtggcc gagcagagaa
    29641 atggtgaatt aaatgtaatt ggcgtgagta atgccaaaag taaaggtgta aaggatggaa
    29701 ttattgttga tattgatgca gcagcaactg ctatcaagtc agccatttcc caagcggaag
    29761 aaaaggcagg catttcgatt aaatcagtga atgtcggctt gcctggtaat cttttgcagg
    29821 tagaaccaac tcaggggatg attccagtaa catctgatac taaggaaatt acggatcaag
    29881 atgttgaaaa tgttgtcaaa tcagctttga caaagagtat gacacctgac cgtgaagtca
    29941 ttacctttat tcctgaagaa tttattgtgg atggtttcca agggattcgt gacccacgtg
    30001 gcatgatggg ggttcgcctt gaaatgcgtg gtttgcttta tacaggacct cgtactatct
    30061 tgcacaattt gcgtaagacg gttgagcgtg caggtgttca ggttgaaaat gttatcattt
    30121 caccactagc aatggttcag tctgttttga acgaagggga acgtgaattt ggtgctacag
    30181 tgattgatat gggggcaggt caaacgactg tcgctacaat ccgtaatcaa gaactccagt
    30241 tcacacatat tctccaagaa ggtggagatt atgtaactaa agatatctcc aaggttttga
    30301 aaacctctcg caaattagcg gaaggcttaa aactgaatta cggggaagcc tatccgcctc
    30361 ttgcaagcaa agaaaccttc caagtagagg ttattggaga agtagaagca gtcgaagtga
    30421 cggaagccta cttgtcagaa attatttctg cacgaatcaa gcacatcctt gaacaaatca
    30481 agcaagaatt agatagaagg cgtctattgg acctccctgg tggtattgtc ttaatcggtg
    30541 ggaatgccat tttaccaggt atggttgagc ttgctcagga agtctttggc gtccgtgtca
    30601 agctttatgt tccaaatcaa gttggtatcc gtaatccagc ctttgcgcat gtgattagtt
    30661 tatcagaatt tgcgggtcaa ttaacagaag ttaatctttt ggctcaggga gcgataaaag
    30721 gtgagaatga cttaagtcat cagccaatta gttttggtgg gatgctgcaa aaaacagctc
    30781 agtttgtaca atcaacgcct gttcaaccag ctcctgctcc agaagtagag ccggtggcgc
    30841 ctacagaacc aatggcggat ttccaacaag cttcacaaaa taaaccgaaa ttagcagatc
    30901 gtttccgtgg cttgatcgga agcatgtttg acgaataaag aggaaaaata aattatgaca
    30961 ttttcatttg atacagctgc tgctcaaggg gcagtgatta aagtaattgg tgtcggtgga
    31021 ggtggtggca atgccatcaa ccgtatggtc gacgaaggtg ttacaggcgt agaatttatc
    31081 gcagcaaaca cagatgtaca agcattgagt agtacaaaag ctgagactgt tattcagttg
    31141 ggacctaaat tgactcgtgg tttgggtgca ggaggtcaac ctgaggttgg tcgtaaagcc
    31201 gctgaagaaa gcgaagaaac actgacggaa gctattagtg gtgccgatat ggtcttcatc
    31261 actgctggta tgggaggagg ctctggaact ggagctgctc ctgttattgc tcgtatcgcc
    31321 aaagatttag gtgcgcttac agttggtgtt gtaacacgtc cctttggttt tgaaggaagt
    31381 aagcgtggac aatttgctgt agaaggaatc aatcaacttc gtgagcatgt agacactcta
    31441 ttgattatct caaacaacaa tttgcttgaa attgttgata agaaaacacc gcttttggag
    31501 gctcttagcg aagcggataa cgttcttcgt caaggtgttc aagggattac cgatttgatt
    31561 accaatccag gattgattaa ccttgacttt gccgatgtga aaacggtaat ggcaaacaaa
    31621 gggaatgctc ttatgggtat tggtatcggt agtggagaag aacgtgtggt agaagcggca
    31681 cgtaaggcaa tctattcacc acttcttgaa acaactattg acggtgctga ggatgttatc
    31741 gtcaacgtta ctggtggtct tgacttaacc ttgattgagg cagaagaggc ttcacaaatt
    31801 gtgaaccagg cagcaggtca aggagtgaac atctggctcg gtacttcaat tgatgaaagt
    31861 atgcgtgatg aaattcgtgt aacagttgtc gcaacgggtg ttcgtcaaga ccgcgtagaa
    31921 aaggttgtgg ctccacaagc tagatctgct actaactacc gtgagacagt gaaaccagct
    31981 cattcacatg gctttgatcg tcattttgat atggcagaaa cagttgaatt gccaaaacaa
    32041 aatccacgtc gtttggaacc aactcaggca tctgcttttg gtgattggga tcttcgccgt
    32101 gaatcgattg ttcgtacaac agattcagtc gtttctccag tcgagcgctt tgaagcccca
    32161 atttcacaag atgaagatga attggataca cctccatttt tcaaaaatcg ttaagtaaat
    32221 gaatgtaaaa gaaaatacag aacttgtttt tcgagaagtt gcagaggcta gtctgagtgc
    32281 tcatcgagag agtggttcgg tctctgtcat tgcagttacc aagtatgtag atgtaccgac
    32341 agcggaagcc ttgcttccgc taggtgttca tcatatcggt gaaaatcgtg tagataagtt
    32401 tctggaaaaa tatgaagctt taaaagatcg agatgtgact tggcatttga ttggtacctt
    32461 gcaaagacgt aaggtgaaag atgtcattca atacgttgat tatttccatg cattggactc
    32521 agtaaagcta gcaggggaaa ttcaaaaaag aagtgaccga gtcatcaagt gtttccttca
    32581 agtaaatatt tctaaagaag aaagcaaaca cggtttttcg agagaggaac tgctggaaat
    32641 cttgccagag ttagccagac tagataagat tgaatatgtt ggtttaatga cgatggcacc
    32701 ttttgaggct agcagtgagc agttgaaaga gattttcaag gcggcccaag atttacaaag
    32761 agaaattcaa gagaaacaaa ttccaaatat gcctatgacc gagttaagta tgggaatgag
    32821 tcgtgattat aaagaagcga ttcaattcgg ttccactttt gttcgtatag gtacatcatt
    32881 ttttaagtag gagagaacca tgtctttaaa agatagattc gatagattta tagattattt
    32941 tacggaggat gaggattcaa gtctccctta tgaaaaaaga gatgagcctg tgtttactcc
    33001 agtaaattct tcacaggaac cggctctccc aatgaatcaa ccttcacagt cggctggtac
    33061 aaaagagaac aatatcacca gacttcatgc aagacaacag gaattggcaa atcagagtca
    33121 gcgtgcaacg gataaggtca ttatagatgt tcgttatcct agaaaatatg aggatgcaac
    33181 agaaattgtt gatttattgg caggaaacga aagtatcttg attgattttc agtatatgac
    33241 agaggtgcag gctcgtcgtt gtttggacta tttggatgga gcttgtcatg ttttagctgg
    33301 aaatttgaaa aaggtagctt ctaccatgta tttgttgaca ccagttaatg ttattgtaaa
    33361 tgttgaagat atccgtttac cagatgaaga tcaacagggt gagttcggtt ttgatatgaa
    33421 gcgaaataga gtacgataat gattttttta attcgtatga tttataatgc agtggatatt
    33481 tactccctga ttttggtagc cttcgctgtc atgtcttggt ttccaggtgc ctacgaatcc
    33541 agtttaggtc gttggattgt agcgttggtg aaaccagtac ttgctccctt gcaacgcctg
    33601 cctttacaga tagcgggtct tgatttatct gtttgggtcg cgattgtttt ggttcgattt
    33661 ttaggagaaa acctagtgcg ttttctggcg atgataggat gaataaaggg atttatcagc
    33721 atttctccat agaagatcgt ccatttcttg acaagggaat ggaatggata aagaaggtag
    33781 aagatagcta tgctcctttt ttaactcctt ttatcaatcc tcatcaggag aagctattaa
    33841 agattttggc caaaacctat ggtcttgctt gtagcagtag tggggaattc gtctcgagtg
    33901 agtatgttcg agttttatta tacccagatt atttccaacc agagttttca gattttgaaa
    33961 tatctctcca agaaattgtg tattccaata aatttgaaca tttaacgcat gctaagattt
    34021 tagggacagt catcaatcaa ttagggattg aacggaaact ttttggagat atcctagtag
    34081 atgaagaacg ggcgcagatt atgattaatc agcagtttct tcttctcttt caagatggac
    34141 taaagaaaat tggtcgtata cctgtttcgc tggaggaacg tcctttcacc gagaaaatag
    34201 ataagctaga acagtatcga gaactggatt tatctgtgtc tagttttcga ttagatgttc
    34261 ttttatcaaa tgttttgaaa ctatctagga atcaagcaaa ccagttgatt gaaaagaaac
    34321 ttgtccaagt aaattatcat gtggtagaca aatcagatta cactgttcaa gttggagact
    34381 tgattagtgt gagaaaattt ggtcgcttga gattacttca agataaggga caaacgaaaa
    34441 aagagaagaa aaaaataacc gtccagttat tattaagtaa gtgaggaata gaatgccaat
    34501 tacatcatta gaaataaagg acaagacttt tggaactcga ttcagaggtt ttgatccaga
    34561 agaagtcgat gaatttttag atattgtggt tcgtgattac gaagatcttg tgcgtgcgaa
    34621 tcatgataaa aatttgcgta ttaagagttt agaagagcgt ttgtcttact ttgatgaaat
    34681 aaaagattca ttgagccagt ctgtattgat tgctcaggat acagctgaga gagtgaaaca
    34741 ggcggcgcat gaacgttcaa acaatatcat tcatcaagca gagcaagatg cgcaacgctt
    34801 gttggaagaa gctaaatata aggcaaacga gattcttcgt caagcaactg ataatgctaa
    34861 gaaagtcgct gttgaaacag aagaattgaa gaacaagagc cgtgtcttcc accaacgtct
    34921 caaatctaca attgagagtc agttggctat tgttgaatct tcagattggg aagatattct
    34981 ccgtccaaca gctacttatc ttcaaactag tgatgaagcc tttaaagaag tggttagcga
    35041 agtacttgga gaaccgattc cagctccaat tgaagaagaa ccaattgata tgacacgtca
    35101 gttctctcaa gcagaaatgg aagaattaca agctcgtatt gaggtagccg ataaagaatt
    35161 gtctgaattt gaagctcaga ttaaacagga agtggaaact ccaactcctg tagtgagtcc
    35221 tcaagttgaa gaagagcctc tgctcatcca gttggcccaa tgtatgaaga accagaagta
    35281 gctccagtgc atccgacagg tccaacacca gctacagaaa ctgttgattc agcaccagga
    35341 tttgaagcac cgcaagaatc cgttacaatt ttataagaaa cgatgtgaga acaatatctt
    35401 atccttatat ttccagcgag caggagatgg tgtgagtcct gcattcccta acgataagat
    35461 tatcctctca aaaactcaag tctgaagcta gtaagatttg acgtttccca cgttacggga
    35521 taagagggag aaagactaaa tctttttccg aataaaggtg gtaccacgat tttcgtcctt
    35581 tttggaagtc gtggttttta atttgttatt atttataaag gagataccat gaaactcaaa
    35641 gataccctta atcttgggaa aacagctttc ccaatgcgtg caggccttcc gaccaaagag
    35701 ccagtttggc aaaaggaatg ggaagatgca aaactttacc aacgtcgtca agaactaaat
    35761 gaagggaaac cgcattttgt cttgcatgat ggccctccat acgcaaacgg aaatatccac
    35821 gttggacatg ccatgaatca tatctcaaaa gatatcatca ttcgttctaa gtctatgtca
    35881 ggttttaacg caccttatat cccaggttgg gatactcatg gtctgccaat cgagcaagtt
    35941 ttggcaaagc aaggtgtgaa gcgcaaagaa atggacttgg ttgagtactt gaaactctgt
    36001 cgtgagtacg ctctttctca agtttataaa caacgtgatg atttcaaacg cttggggatg
    36061 tcaggtgact gggaaaattt atatgtgact ttaactccag actatgaagc agcccaaatc
    36121 cgtgtctttg gtgagatggc taataagggt tatatctacc gtggtgccaa gccagtttac
    36181 tggtcatggt catctgagtc agcccttgcc gaagcggaaa ttgaatacca tgatttggtt
    36241 tcgacttcac tttattatgc caataaggta aaagatggca aaggtatcct agatacagat
    36301 acttatatcg tagtctggac aacaactcca tttaccatca cagcttctcg tggtttgact
    36361 gttggtgcag atattgatta cgttttggtt caacctgcta gtgaaactcg taagtttgtc
    36421 gttgctgcag aattgttgac tagcttgtct gagaaatttg gctgggctga tgttcaagtg
    36481 ttggcaactt accgtggtca agaactcaac cacatcgtaa cagaacaccc atgggataca
    36541 gctgtagatg agttggtaat ccttggtgat cacgttacca ctgactctgg tacaggtatc
    36601 gtccatacag cccctggttt tggtgaggac gactacaatg ttggtattgc taatggtctt
    36661 gaagtcgcag tgactgttga cgaacgcggt atcatgatgg cgaatgctgg tcctgagttt
    36721 gaaggtcagt tctacgacaa ggttgtacca actgttattg aaaaacttgg taacctcctt
    36781 ctagcccaag aagaaatctc tcactcatac ccatttgact ggcgtactaa gaaaccaatc
    36841 atctggcgtg cagttccaca atggtttgcc tcagtttcaa aattccgcca agaaatcttg
    36901 gatgctattg ataaagttaa attccataca gaatggggta aagtacgtct atataatatg
    36961 attcgtgacc gtggtgactg ggttatctct cgtcaacgtg cttggggtgt tccacttcca
    37021 atcttctatg cagaagacgg tacagctatc atgacagctg agactattga acatgtggcg
    37081 caactctttg aagtacatgg ttcaagcatt tggtgggaac gtgatgccaa agacctctta
    37141 ccagaaggat ttacacatcc aggttctcca aatggggagt tcaagaaaga aacagatatt
    37201 atggatgtat ggtttgactc aggttcatca tggaatggtg ttttggtaaa tcgtccaaat
    37261 ctgacttatc cagctgacct ttaccttgaa ggttctgacc aataccgtgg ttggttcaac
    37321 tcatcactca ttacatctgt tgccaaccat ggcgtagcac cttacaaaca aatcttgtca
    37381 caaggtttta cccttgatgg taaaggtgag aagatgtcta aatcacttgg aaataccatt
    37441 gctccaagtg atgttgaaaa acaatttggt gcagaaatct tgcgtctctg ggtaacaagt
    37501 gttgactcaa gcaatgacgt gcgtatctct atggatattt tgagccaagt ttctgaaact
    37561 taccgtaaga ttcgtaacac tcttcgtttc ttgattgcca atacatctga ctttaaccca
    37621 gctcaagatg tagtcgctta cgatgaactt cgttcagttg ataagtacat gactattcgc
    37681 tttaaccagc ttgtcaagac cattcgtgat gcctatgcgg actttgaatt cttgacaatc
    37741 tacaaggcct tggtgaactt tatcaacgtt gacttgtcag ccttctacct tgattttgcc
    37801 aaagatgttg tttacattga aggtgccaaa tcattggaac gccgtcaaat gcagactgtt
    37861 ttctatgaca ttcttgtcaa aatcaccaaa ctcttgacac caatccttcc tcacactgcg
    37921 gaagaaatct ggtcatatct tgagtttgaa gctgaagact tcgttcaatt gtcagaattg
    37981 ccagaagctc aaacttttgc taatcaagaa gaagtcttgg atacatgggc agccttcatg
    38041 gacttccgtg gacaagctca aaaagccttg gaagaagctc gtaatgcaaa agttatcggg
    38101 aaatcgcttg aagcacactt gacagtttat ccaaatgaag ttgtgaaaac tctactcgaa
    38161 gcagtaaaca gcaatgtggc tcaacttttg atcgtgtcag acttgaccat cgcagaaggg
    38221 ccagctccag aagctgccct tagcttcgaa gatgtagcct tcacagttga acgcgctgca
    38281 ggtcaagtat gtgaccgttg ccgtcgtatc gacccaacaa cagcagaacg cagctaccag
    38341 gcagtcatct gtgaccactg tgcaagcatc gtagaagaaa actttgcgga agcagtcgca
    38401 gaaggatttg aagagaaata aggttgaaaa gtctaggcaa aattcaattt gagaagaaaa
    38461 gacaactaat tttatagtct attaaacgca ttgtatcacg tttttgaata cctgatatga
    38521 tgcgtttttt atttatttta aaaatttgcg aggtatgact ttttatactc aacaagaatc
    38581 aaagagaaac ttagcaagct aacagtagta agataaaata ggaatttgat attagggata
    38641 agattgctaa atagtgtaat atttttacaa taataaattt atatagttat ttttggtttc
    38701 tgaaaagtat tatattttat ttcatattat acaaattttt attttataat atcagaaaat
    38761 acttttttta aaagcaaata tgatacaatt ttatttgaaa aaaataaaaa aggagatttt
    38821 attatgaaat taaaaacact tgctttaatt agtggtatcg tcggtcttgt gggaggaatt
    38881 ttacttctta ttggtccttt tgtcttgttg ggaacagcgg taaacacagt tgctacaact
    38941 cttaatggag gagctactgc aggggctttt tcaggtgttg ccttactctt gaatgccttg
    39001 aagattgcaa atcttgttct tggtatcatt gctattgttt actataaagg agataatcgt
    39061 gtaggtgcag ctccgtctgt actaatgatt gtttttggtg gagttagtct cattccattc
    39121 ttaggatggg ttggggggat tcttgttatt atcggaggat ctctattcct tgcaacattg
    39181 aagaaattca aatcagaaga ataaaaggta ttttagcatg aaaagaacaa aaaagtttat
    39241 cggtatagga gtagctctat tatctctttt tcttctagtt gcatgtggaa cataaagttc
    39301 aaaggatact tcaacaagta atgatgagaa gacagtagca acatccaata gttcaaaaga
    39361 aacaatcact ttcgatacac cggttgtaac agacgatgtg attgaatcaa tacgcactta
    39421 tgcagattat atagatcttt ataaaaatat ttttgatgat tattttacta aagctgagga
    39481 aggtttcaaa ggcacagcta tggaaaataa tgactcgttt actaaactaa aagagtcaac
    39541 tcaaaaatta ttcgatgcgc agaaaaaaag gttaaataat gaagatagaa tagaaacaac
    39601 taaaaacaat gtgattgcca aacattgtca aacagtcctt tcctttttgg ttttgactag
    39661 cttttttgtg aaaaattgtg taaaatagaa tagataaacg aggggaaacc tcggaaaatt
    39721 taaaggagaa tccatctaat ggtaaaattg gtttttgctc gccacggtga gtctgaatgg
    39781 aacaaagcta accttttcac tggttgggct gatgttgatt tgtctgaaaa aggtacacaa
    39841 caagcgattg acgctggtaa attgatcaaa gaagctggta tcaaatttga ccaagcttac
    39901 acttcagtat tgaaacgtgc tatcaaaaca actaacttgg ctcttgaagc ttctgaccaa
    39961 ttgtgggttc cagttgaaaa atcatggcgc ttgaacgaac gtcactacgg tggtttgact
    40021 ggtaaaaata aagctgaagc tgctgaacaa tttggtgatg agcaagttca catctggcgt
    40081 cgttcatacg atgtattgcc tccaaatatg gaccgtgatg atgagcactc agctcacaca
    40141 gaccgtcgtt atgcttcact tgacgactca gtaatcccag atgctgaaaa cttgaaagtg
    40201 actttggaac gtgctcttcc attctgggaa gataaaatcg ctccagctct taaagatggt
    40261 aaaaacgtat tcgtaggagc tcacggtaac tcaatccgtg cccttgtaaa acacatcaaa
    40321 ggtttgtcag atgacgagat catggacgtg gaaatcccta acttcccacc attggtattc
    40381 gaattcgacg aaaaattgaa cgtcgtttct gaatactacc ttggaaaata aaaaattgta
    40441 agtctagaat tgatttctag gctttttatg ttagtatgga agtatgataa ggaataaaaa
    40501 acaagattat gtactggcct acaagcaacc agtttcaacc acttacatgg gttgggaaga
    40561 agaagcttta ccgataggca atggttcttt aggagcaaaa gtatttggcc ttataggggc
    40621 tgaacggatt caatttaatg aaaaaagtct ctggtctgga ggtccacttc ctgatagttc
    40681 agattatcag ggtggaaatc ttcaggatca gtatgttttt ttagctgaga ttcggcaggc
    40741 tttggagaag agagattaca atctggctaa ggaactggct gagcagcacc taattgggcc
    40801 aaaaacgagt caatatggga cctatctgtc ttttggggat attcacattg agttcagcca
    40861 gcaaggtacg actttgtctc aggtgacgga ctatcagaga cagctgaata ttagtaaggc
    40921 acttgtgacg acttcttatg tctataaggg aacgcgattt gaacgtgaag cttttgcgag
    40981 ttttccagat gatctcttgg ttcagcgttt tactaaggaa ggggcggaaa ctctagattt
    41041 tactatagaa ctatccttga cctgtgattt ggcttctgat ggaaagtatg agcaggaaaa
    41101 atctgattac aaggagtgta agttggatat tactgattct catatcttga tgaagggaag
    41161 agttaaggat aatgatctgc ggtttgctag ttatctagct tgggaaacgg atggagatat
    41221 tagagtttgg tcagataggg ttcagatatc aggagccagt tatgccaatc tcttcttggc
    41281 cgctaagacg gattttgccc aaaatcctgc tagcaattat cgcaagaaac tagatttaga
    41341 gcaacaggtg atagacttgg tggacacagc taaagaaaag ggctataccc aattgaaatc
    41401 aaggcatatc gaggactacc aagccttatt ccagcgtgtt caattggatt tggaagctaa
    41461 tgttgacgca ttcactacag atgatttgtt aaaaaattat aagccacaag aagggcaggc
    41521 tttggaggag ctgttcttcc agtatggacg gtatttattg attagttcgt ccagagactg
    41581 cccagatgct ctaccagcta acctacaggg agtctggaat gcggtcgaca atcctccttg
    41641 gaattcggac tatcacttaa atgtcaatct gcagatgaat tattggccag cctatgttac
    41701 caatctccta gagacggtct ttccagtcat caactatgta gatgatttgc gtgtctatgg
    41761 tcgtctagcg gctgtaaagt atgcaggaat cgtctctcag aaaggtgagg agaatggttg
    41821 gttggttcat actcaagcga ctccctttgg ttggacggca cctggttggg attactattg
    41881 gggttggtca ccagctgcca atgcgtggat gatgcaaacc gtttatgaag cctattcatt
    41941 ttatagggac caagactatc tcagggagaa aatttatccc atgttgaggg aaacggttcg
    42001 tttttggaat gcctttttac ataaggatca gcaggcgcag cgttgggtgt cttctccgtc
    42061 ttattcccca gaacatgggc cgatttcgat tggcaatact tatgaccaat ctctgatttg
    42121 gcagttattt catgatttta ttcaggctgc tcaggaattg ggactggatg aggacttgtt
    42181 gactgaggtt aaggagaagt ctgatttact aaatcctttg caaatcactc aatctggtcg
    42241 aatcagggag tggtatgagg aggaagagca gtattttcaa aatgagaaag tggaggccca
    42301 gcatcggcac gcttcccatc tagtgggact ctatcctggc aatctcttta gctacaaggg
    42361 acaagagtat attgaagcgg tacgtgctag cctcaatgat cgtggagatg gcggcacagg
    42421 ctggtccaag gctaataaga tcaatctctg ggcgcgtttg ggagatggca atcgagccca
    42481 taaattattg gcagagcagt taaagacatc caccttgcca aatctttggt gtagccatcc
    42541 tccttttcag atagatggta attttggtgc tactagtggc atggcagaaa tgttactcca
    42601 gtctcatgca gcttatctgg tacctctagc tgccctacct gatgcttggt caacaggttc
    42661 tgtttcaggc ttaatggcac gtggacattt tgaagtgagc atgagctggg aagataaaaa
    42721 actcttacag ttgaccattt tatcaaggag tggaggagat ttgcgagttt cttatccaga
    42781 tattgagaag agtgtgatta aaatgaatca agaaaaaata aaagcgaaat gcatggggaa
    42841 agattgtatt tcggtggcaa cagcagaagg tgatcttgtt caattttatt tttaagaaga
    42901 tgttataagg cagtaatttg aaactgcctt ttaataagga tttaagaata taagcagttt
    42961 tcaactagtt gaaaaaacgt tataatgata ataggaagta atactcaatg aaaatcaaag
    43021 agcacaaact aggaagctag ccgcaggttg ctcaaaacag tgttttgagg ttgcagatgg
    43081 aagctgacgt ggtttgaaga gagattttcg aggagtataa tttgtttgat agagggtggg
    43141 tctgatggct tatattgaga tgaaacactg ttacaagcgt tatcaggttg gggacacgga
    43201 gattgtggcc aattgtgatg tgaattttga gattgaaaag ggggagctgg ttattatcct
    43261 tggtgcttca ggtgcaggca agtcaacagt tcttaacctt cttgggggaa tggataccaa
    43321 tgatgaaggg gaaatctgga ttgatggtgt taatattgcg gattatagtt cccaccagcg
    43381 caccaattac cgtagaaatg atgtggggtt tgtttttcag ttttataatc tagtttctaa
    43441 tctgacagct aaggaaaatg tggaactggc ttctgaaatt gtgacagatg ccttgaatcc
    43501 tgatcaggtc ttgacagatg taggtctggc tcatcgtctc aataactttc cagcccagct
    43561 ttctggaggg gagcaacagc gagtctccat tgcacgcgcg gtagccaaaa atcctaaaat
    43621 tctcctttgt gatgaaccga ctggagcctt ggattatcag acgggcaagc aggttttgaa
    43681 aattctccaa gacatgtctc gtcaaaaggg agcgacggtg atcatcgtga ctcataatgg
    43741 agctttggcg cccattgctg atcgcgtgat tcaaatgcac gatgccagtg tcaaggatgt
    43801 ggtgctcaac cagcatcctc aggatattga cagtttggag tactagcatg atcaagcgaa
    43861 aaacttattg gaaggactta attcagtcct tcacaggctc caaggggcgt tttttatcca
    43921 tcttgatcct gatgatgttg ggatctctag ccttagtagg cctcaaagta accagtccca
    43981 acatggaggc gacagctaat gcttatttaa caactgctca aaccttggat ttggcagtca
    44041 tgtctaacta tggcttggat caagcagacc aagaagaact aaaacagacg gagggcgcag
    44101 aggtcgagtt tggctatttg acagatgtga ctatggataa tgggcaggat gccattcggc
    44161 tgtactccaa accagagcga atttcaacct ttcagctaag aaagggacga cttcctcagt
    44221 cagacaagga aatcgctttg gccactcatt tgcaaggcca atacagcgtg ggacaggaga
    44281 ttagttttaa agaaaaagaa gagggtcatt cctctttaaa agaccatact tataccatta
    44341 ctggttttgt ggattcggct gaaatcctct cccagcgaga tatgggctac gcaggaagtg
    44401 gaagtgggac tctgacagcc tatggggtga ttttacctag tcagtttgat cagaaagtct
    44461 acaatatagc tcgtttgaaa tatcaagatt tagcaggttt aaatgccttt tcatcagctt
    44521 atgaagaaaa atccaagcaa catcaggaag accttgaaca aactttatca gataatggca
    44581 aggtacgtct gcaacttttg aaaaaagaag gacaagagtc tctagacaag gggcaagaga
    44641 cccttgacaa ggctcagact aatttgcagg aaggcaagcg tcgtttagca gctgctcaag
    44701 ctcgtataca ggctcaagaa agtcaactag ccttgtttcc tcaagttcag agagagcagg
    44761 ctagtgctca acttacccaa gccaagcagg aattgggcaa ggaagaggac aaactaaagc
    44821 aagctgaaca aaatctagcc caagaaaagg aaaaattaga aaaacatcag caagtcttgg
    44881 acgatttggc ggagccaagg tatcaggtct ataatcgtca gaccatgcca ggtggtcagg
    44941 gctatcttat gtatagcaat gcttcatcca gtattcgagc agtgggcaat atctttcctg
    45001 tggtacttta tgccgtagca tccatggtga cctttacgac catgactcgc tttgtagacg
    45061 aagagcgaac tcatgcaggg atttttaagg ccttgggtta ccgtagtaag gatattatca
    45121 ccaagtttct cctttatgga ctagtagctg ggactgtcgg aacggctcta ggtagtatac
    45181 ttggtcatta tttgctagcc agtgtaattt caagtgtcat tacaaaaggc atagtggtgg
    45241 gagaaaccca gattcagttc tattggacct atagcttact agctcttgtc ttgagttggt
    45301 tggcgagtgt gttaccagtc tatctggtgg cttggaggga acttcatgcc gaagcagccc
    45361 agcttctact tcctaaacct cctgtcaaag gagctaaaat cttattggag cgtatcggtt
    45421 ttatctggcg tcgtctcagt tttactcata aggtaacagc ccgcaacatc tttcgttata
    45481 agcagagaat gttgatgaca atctttggtg tggcaggttc tgtagctctg ctctttgcag
    45541 gtttgggaat ccaatcttct gtagcaggag ttccgtctaa acagtttcaa caaatccaac
    45601 agtatcagat gcttgtctct gaaactccta gtgcgaccaa tcaggacaag gtagagctag
    45661 cagaagtgtt gaaagggcag gagatactag cctaccagaa aatctattct aaaacgctag
    45721 acaaggattt caaaggcaag gctggtcttc aaaacattac tcttatgatg atagagaagg
    45781 aagatttgac tccctttatc catcttcaac atcatcagca ggagctgaca ttaaaagatg
    45841 gcatcgttat tacagctaaa ctcgcccagc tggcaggtgt caaggttggg cagactttag
    45901 aaattgaagg taaggaacta aaggtcgctg ctattactga gaactacgtt ggtcacttta
    45961 tttatatgag tcaggctagc tatgagcaac tttacggaca gctaccccaa gccaacactt
    46021 atctggtctc attaagggat accagtgcaa ctagtatcga aagtcaggcg ggcttgctta
    46081 tgaatcaatc tgcggtgtcc agcgttgtcc aaaatgcttc agccattcga ctcttcgact
    46141 ctatcgctag ctcactcaat cagaccatga ccatcttggt catcgtatcg gttctattag
    46201 ctattgtcat cctttacaat ctgaccaata tcaacgtagc tgagagaatc cgtgaactct
    46261 ccactatcaa ggttctcggt tttcataata atgaagtcac cctctacatt taccgtgaga
    46321 cgattgtgct gtcccttgtg ggaatcgtac ttggtctggt agctggtttc tatttacacc
    46381 aatttttgat tcaaatgatt tcgcctgcga ctattctctt ttatccgcag gtaggctggg
    46441 aagtctatgt aatcccagtg gcagcagtaa gcatcatttt gaccttgctt ggtttcttcg
    46501 tcaattatta tctgagaaag gttgatatgt tagaagccct gaaatctgta gagtaaggta
    46561 gttattttta gctgattgaa cttctattta ttaatattca aaaatcctcc gtttcaaaga
    46621 gcagggaact ctttgtgaca gaggattttt tctatagggc tttagcagct gcaattgcgg
    46681 cttcgaagtt tggctcagaa ttgatattat ccacgtattc aacgtagcga atcgtattgt
    46741 cagtatcgag gacaaagact gcgcgtgcta ataggtgcca ttcgttgatc aagagggcat
    46801 aatcgcgccc gaaagaatgg tcaaagtagt ctgaaagcat aatggcattg tcaaggcctt
    46861 cagcaccgca ccaacgtttt tgagcaaaag gtaggtccat tgaaacagtc aatacgaccg
    46921 tgttgtccag tccagctaat tcttcattaa aacgacgtgt ttgagttgag cagatgcctg
    46981 tatcgataga aggaacgaca ctcaggactt ttttcttgcc atcaaaatca gccagagatt
    47041 ttttagaaag atctgttgta gtaagagaaa aatcaagcgc cttgtcgccg ccttgtagtt
    47101 gtttacctgt aaagctcaca ggatttccga gaaaagttac cataggatac tccaatcttt
    47161 tttcttccat tgtatctgaa acagtcagaa ttttccaatg atttgaccgg aaatgtgggc
    47221 atagaaaaaa cgccagctca catgagaatg acgtttttca gaggcttatt ttgccaatcc
    47281 ttcagcaatc ttgtcaaggt tgtatttcat catgctgtag tagctgtcgc cttctttacc
    47341 ttgttctgcg atagagtcag taaagatttg tgcgtagatt gggatgtttg tgtcttgaga
    47401 aacagttttc attggacggt catccacact tgattctaca aagagtgatg gaacttttgt
    47461 ttggcgaagt ttttcaacca aggtcttgat ttgttcagga gttccttctt cttcagtatt
    47521 gatttcccag atgtaggcac ttgggacacc ataggcttta gagaagtatt tgaatgctcc
    47581 ttcgctggtt acaatgagtt tcttttcagc agggatctta ttaaatttat ccttactttc
    47641 tttatcaagt ttgtctaact tatcagtata ttctttgaga tttttttcat agaattcttt
    47701 attgttaggg tctttggcgc tcaattgttt ggcgatattt ttagcaaaaa taataccgtt
    47761 ttcaaggtta agccaagcgt gtgggtcttc ttttcctttt tcattttgac cttcaaggta
    47821 gataacatca acgccgtcgc tgactgcgaa gtagtctttg ttttcagttt tcttggcatt
    47881 ttctaccaat tttgtaaacc aagcattgcc acctgtttca aggttgatac cgttatagaa
    47941 aatcaaatca gcctcagaag ttttcttaac gtcttcagga agtggttcgt attcgtgtgg
    48001 gtcttgccca atcggaacga tactatgaag gtcaattttg tcaccagcaa tatttttagt
    48061 aatatcagcg atgattgagt ttgtagcaac aacttttagt ttttgaccag aagttgtatc
    48121 tttttttccg ctagcacatg ctacaagaat gattgcagaa agaaagagaa cgagtaatgt
    48181 acctaatttt ttcattagat cctccaattt attagggctt tgccccttat tttaacaaat
    48241 gtttattttt cagtttcaaa tatcgttgtt tgggagcgat aaagaagcta atgagaaaga
    48301 aactagcagc tgtaagcacg atactagaac ctgccgcaac attaaaacta tagccaataa
    48361 agagtcccaa aactgaagca gtagctccga aggttgagga aaggaaaatc atgcttttca
    48421 gactattagc atacagataa gcagttgcag ctggggtaat cagcatggct acaatcagga
    48481 tagttccgac actttgcatg gctgtcacag acacgagagt caggagtacc atgagaaggt
    48541 agtgatagaa attgacaggc attcccatgg ctttagccaa gagttcatca aaggaagtta
    48601 tcaagagttg cttgaagaaa atccagatta acaagaggat ggctgccccc acacccatag
    48661 taataaacat atccgtatct tggacggcca ggatattacc aaaaaggata tggaaaaggt
    48721 cagttgaact tttagcgaca ccaatcaaga tgataccgag ggctaagaaa gaagaaaagg
    48781 taatgccgat ggcggtatcg cttttgataa tcgagtttcc tttgatgtag gtaatgatga
    48841 tggcagctag caatccaaag acaatggctc cgataaagaa gtcaaggccc aagatgaagg
    48901 agagggctac acctggtaag acagcatgtg aaatggcatc tcccatgagt gacatcccgc
    48961 gtagaataat gaaacatccc acagctccag ctacaatccc gacgacaata gctgttatca
    49021 aggcattttg taggaaatgg aatttttgca atccatcgat aaattctgca atcataggtc
    49081 acctccattg aaaaagagtt gattaccgta agcttctttt agattggttt cggtaaaagt
    49141 ttcttttgtt ggaccaaagg caatcacttc tcgattgaca agtaagactt gatcgaagta
    49201 gtggggaatc ttgctgaggt cgtggtgaac gatgagaacc gtcttcccag cttttttcaa
    49261 atctctcagc gtattcatga tgatttcctc actgacagag tcaatcccag caaagggttc
    49321 atccaagagg atatagtcgg cttcctgcac caaacatctg gcaatcaaga cccgctggaa
    49381 ttgacctcca gacagttgac taatttgacg ttcagcgtag tcagctaggc cgacgatttc
    49441 aagggcctct tgcactttct tccaatgttt agcctttaaa cttcgaaaga gaggaataga
    49501 gggaaatagt cctaacgaga cgcattcctt gaccttgatg ggaaagttgt agtcgatatt
    49561 gattttttgt tcgacatagg caattcggtg taaggatttt ttaacttcct tgtcatcgag
    49621 aaatgcctga ccttgatgtg ggataattcc cagcatacct tttaatagtg ttgatttccc
    49681 agcgccgttt ggaccaatga tgccggtaat tgttggtcca tggagcacta gtgaaatatc
    49741 cttaagtgcc aacgtttctt tgtaggagac actgaggttt tcgatacgta tcataaactt
    49801 gtattcctcc tgtctcttaa tatacattaa aaaaaaaatt aagtcaagtt aatttttgaa
    49861 aaaattaaaa taataactga aaaatagatt ctaaagataa ctttcaggat aaatttctaa
    49921 attataaaac gcatagtatc aagtgtaaaa aacttggaat tatgcgtttt atcatggaaa
    49981 gattttttat aatagctaaa aaataatagt taaatggtta ttaattgcat tccctagtga
    50041 tttttgttaa gataaatgca aatacaaatg aaagcgagaa caagatgaca cgttatcaag
    50101 atgattttta tgatgctatc aatggaaaat ggcaacagac agctgaaatc ccagcagata
    50161 agtctcaaac aggaggtttt gttgatttag accaggaaat tgaagacctg atgctggcga
    50221 caacagacaa gtggttagca ggtgaagaag tgcctgagga tgctatcttg gaaaactttg
    50281 tcaaatacca ccgcctagtt cgtgattttg acaagagaga agctgacggt atcacacctg
    50341 tcttaccact ccttaaagaa ttccaagaat tggaaacttt tgcggatttt acagctaaac
    50401 tagcagagtt tgagcttgca ggaaaaccaa acttccttcc ttttggtgta tcgccagact
    50461 ttatggatgc tagaatcaat gttctatggg ctagcgctcc aagcacaatc ttgccagata
    50521 cgacctacta tgcagaagaa catcctcagc gcgaagagct cttgactctt tggaaagaaa
    50581 gcagcgcaaa tctcctcaag gcttatgatt tctctgatga agaaattgaa gacttgctag
    50641 aaaaaagact tgaattcgac cgccgagttg cggcagtggt gctctctaat gaagaaagtt
    50701 cagaatatgc taaactctat catccatatt cttacgaaga tttcaagaaa ttcgcgcctg
    50761 ccctaccttt ggatgacttc ttcaaagcag ttattgggca attaccagac aaggttattg
    50821 tagacgagga acgtttctgg caagcagcag agcaatttta cagtgaggaa gcctggtctc
    50881 tccttaaagc aaccttgatt ttgagtgttg tcaatctttc aaccagctat ttaacagagg
    50941 atatccgtgt tttgtctggt gcctacagcc gtgccctttc tggagttcca gaggcaaaag
    51001 ataaggtcaa agcagcttat catctagcac aggaaccttt caagcaagcc ctggggcttt
    51061 ggtacgcccg tgagaagttc tctccagaag ccaaggcgga tgtggagaaa aaagtggcaa
    51121 ccatgattga tgtctataag gagcgtctgc ttaagaatga ctggctcact ccagaaacct
    51181 gtaaacaggc tatcgtgaag ctcaatgtga tcaaacctta tattggctat ccagaagaat
    51241 tgcctgcacg ttacaaggat aaggtagtga atgaaactgc cagtcttttt gagaatgctc
    51301 tagcctttgc gcgtgtggaa atcaagcaca gttggagtaa gtggaaccag cctgtagact
    51361 ataaggaatg gggcatgcct gctcatatgg tcaatgccta ctacaatcct cagaagaacc
    51421 tgattgtctt tccagcggcc attttacagg cgcctttcta tgacttgcat cagtcatctt
    51481 ctgctaacta cggtggtatt ggggcagtga ttgcccatga aatttcccac gcctttgata
    51541 ctaacggggc ttcctttgac gaaaatggta gcctcaagga ttggtggaca gagagcgact
    51601 atgctgcctt caaggagaaa acacaaaaag tcattgacca atttgatgga caggattctt
    51661 atggagcaac cattaacggt aaattgactg tatcagaaaa cgtggctgac ttgggaggaa
    51721 tcgcagcagc gcttgaagca gctaagagag aagcagactt ctcagcagaa gagttcttct
    51781 acaacttcgg tcgcatctgg cgcatgaaag gtcgaccaga atttatgaaa cttttggcta
    51841 gcgtcgatgt gcacgcacca gccaaactcc gtgtcaatgt gcaagtacca aacttcgacg
    51901 atttctttac aacctatgat gtcaaagaag gagacggaat gtggcgttca ccagaggagc
    51961 gcgtgattat ttggtaatac aaaaaatcta gtcatcagaa aaaaggcatc caatcacgtg
    52021 tgaaaacctt gataggatgc cttttgtaat agaaagattt gctggatagt ttacttaaat
    52081 ttcgattgtc atcatcttgc tagaaaaagg gattgaaggc cttttcgtga gcgatagtag
    52141 tagctggacc atgtcctgga tagacatcgt agtttggtag ggtgaagagt tgggtctgga
    52201 tactatgaag gagttgctcc atgctaccag tcggaaggtc ggtccgtccg atagtttcgc
    52261 ggaatagagc atctcccgtc aagactaggt gagcatcagg aaagactagg gaaacaccac
    52321 cgatagagtg ccctggggtc ggtagaacct taaaacgaaa ttcctctagt tggtattctt
    52381 cgtgaaagac aaaggtgtgt tctgcaggtt ttgtgaccac atctgccata tcatcgtggc
    52441 gagggagacc ggagagatta tcgacaggag tgtagagcca gctggcttcg ctctctgcga
    52501 tatagacagg aggattgcca aacgtctcgc gaaccaagtc cagactcatg atatggtcat
    52561 aatgggcgtg ggtcaagaga atagcacaga tcggtttgtt gatcttctcg attgtctgac
    52621 gaatggcttc ccaatgacta ccaggatcga cgacgatgag gtgcttttcg ccttctagat
    52681 aataggtatt ttcataggca acaggattca cggttttatg gattttcata ctaactccaa
    52741 tctcaaagaa tgtactaata ctcttcgaaa atctcttcaa accgcgtcaa ctttatctgc
    52801 aacctcaaag cagtgctttg aacagcctgc ggctagcttt ctagtttgct ctttgatttt
    52861 cattgagtat aaattaagta tagcacagtt agggagaata ggtaaggatt taaaatgaac
    52921 ttaaaaacca gcatgactag atgaagacag gctggttatc aatttatcaa tattggaaag
    52981 gtgtcaattg cccttcatag gttgcataaa ctccgtcatt agcttgtttg atagacgtga
    53041 tgtttagagt gctaccgtag ttggcttctc atttgtcgct atattcacta taagttatcc
    53101 tactgggcag gttctggtcg ttagcatagt attgaacgag ttttttatag ctttgctggc
    53161 tgaataggaa caggcagcaa actcctaaca ttaggagagc aaagatagaa atagcagttt
    53221 tttcataggt attctcctta taaactaaag gatagaagaa agtatcttga ggatttttat
    53281 actcttcaaa aatctcttca aaccgcgtca acgtcgcctt gccgtatata tgtgactgac
    53341 tttgtcagtc ttatctacaa cctcaaagca gtgctttgag caacctgtgg ctagtttcct
    53401 agtttgctct ttgattttca ttgagtatta attccaaaat ccagtcaagg taccagtgta
    53461 tgtgatacga gttccaccgt ttatctttcg tatcttcaat ggaattgggg aaatcagaaa
    53521 gtctttgggt tacaatatgg tttccgttct tctatctctc cttctatcac ctaagtttaa
    53581 aatctaactt cgtagtatat tatgggccaa taattcagaa ttatcaagca attttgaatt
    53641 atttttttct tattctgttc caatctagtt ttcatattct taaaaaagtg ataaaatggt
    53701 aggaagaatt ggagagtaga gatgccgaaa gaagtgaatt taacaggcga agaagttgtc
    53761 gctttaacca aagaatattt aacggaagag gatgttcatt ttgtccataa ggccttggtc
    53821 tatgctgttg aatgccacag tggtcaatat cgcaaatcag gcgagcctta tatcattcac
    53881 cctatccaag tggcaggtat tttagctaag ctaaagctgg atgctgtaac agtagcttgt
    53941 ggattcttgc atgatgtggt ggaagataca gatgcgacct tggacgattt ggaaagagag
    54001 tttggtcctg atgtgcgggt gattgttgac ggagttacca agcttggcaa ggtcgagtac
    54061 aaatcgatcg aggagcaatt agcggaaaat catcgcaaga tgctcatggc catgtctgag
    54121 gacatccgcg ttattttggt caaactgtct gaccgcttgc acaatatgcg gaccctgaaa
    54181 catcttcgaa aagacaagca ggagcgtatt tccaaagaaa ccatggaaat ctatgcccca
    54241 cttgcccatc gtttggggat ttccagtgtc aaatgggaat tagaagactt gtctttccgt
    54301 tatctcaatc caacggagtt ttacaagatt acccatatga tgaaggaaaa gcgcagggag
    54361 cgtgaggcct tggtggatga ggtagtcaca aaattagagg agtatacgac agaacgtcac
    54421 ttgaaaggga agatttatgg tcgtcccaag catatttact caattttccg caaaatgcag
    54481 gacaagagaa aacggtttga ggaaatctat gatctgattg ctattcgttg tattttagat
    54541 acccaaagtg atgtttatgc catgcttggt tacgtgcatg aattttggaa accgatgcca
    54601 ggtcgcttca aagactatat tgccaaccgc aaggccaatg gttatcagtc tatccatacg
    54661 actgtttatg gaccaaaagg gccgattgaa ttccagattc gaaccaagga aatgcacgag
    54721 gtggctgagt acggggttgc ggctcactgg gcttataaga aaggtataaa ggggcaagtt
    54781 aacagcaagg aatcagctat tggaatgaac tggatcaagg agatgatgga gctccaagac
    54841 caggctgatg atgctaagga atttgtggac tctgttaagg aaaactattt ggctgaggag
    54901 atttacgttt ttaccccaga tggagctgtc cgttcccttc ccaaagattc aggaccgatt
    54961 gattttgcct acgaaatcca taccaaggtc ggtgaaaaag caactggtgc caaggtcaat
    55021 ggccgcatgg ttccactgac aaccaagtta aagacagggg atcaggttga aattatcgcc
    55081 aacccgaact cctttggacc tagccgtgac tggctcaata tggtcaagac tagcaaggcg
    55141 cgcaataaga ttcgccagtt ctttaaaaac caagataagg aattgtctgt caacaagggt
    55201 cgtgagatgc tgatggctca gttccaagaa aatggctatg tggcaaataa atttatggac
    55261 aagcgccaca tggatcaagt tctgcaaaag accagttaca agacagaaga ctccctcttt
    55321 gcggccattg gttttgggga aatcggtgcg attaccgtct ttaaccgtct gactgaaaag
    55381 gaacgccgtg aggaagagcg tgccaaggcc aaggctgagg cagaggagct tgtcaaaggt
    55441 ggcgaggtca aggttgaaaa taaagaaact ctcaaggtca agcatgaggg gggagtggtt
    55501 attgaaggtg cttctggtct cctagtgcgg attgctaagt gttgtaaccc cgtgcctggt
    55561 gacgatattg ttggctacat taccaagggt cgtggtgtgg ctattcaccg tgtggactgt
    55621 atgaacctgc gtgcccaaga aaactacgag caacgtctcc ttgatgtgga atgggaagac
    55681 cagtactcta gctcaaataa ggagtatatg gcccatatcg atatctacgg tctcaaccgt
    55741 acaggactgt tgaacgatgt actgcaagtt ctttcaaata caaccaagaa tatttcaacg
    55801 gtcaatgccc aaccaaccaa ggatatgaag tttgctaata tccatgtgtc cttcggtatt
    55861 gccaacctct ctacactgac cacggttgtc gataaaatta agagtgtgcc agaagtttac
    55921 tctgtcaaac ggaccaacgg ctagttgtcc tagctcttac tagaaaggct attatgaaaa
    55981 tcattatcca acgggttaaa aaagcccaag tgagtataga aggccagatt cagggaaaaa
    56041 tcaatcaggg acttttattg ctggttggtg ttggaccaga ggaccaagag gaagatttgg
    56101 actatgctgt gagaaaactg gtcaatatgc ggattttttc agacgcagaa ggcaagatga
    56161 acctgtctgt caaagatatt gaaggagaaa tcctctctat ttctcagttt accctctttg
    56221 cggatactaa gaaaggcaat cgtccagcct ttacaggggc agctaaacct gatatggcat
    56281 cagacttcta tgatgctttc aatcaaaaat tagcgcaaga agtgcccgtt cagacaggta
    56341 tctttggagc agatatgcag gttgagctgg ttaataacgg acctgttacc attatcctag
    56401 atactaaaaa gagataagaa agaccaagcc cagtcggctt ggtctttctc atcgatcata
    56461 aaatactcca aaaagaaatc ggttcttgat atgcttgggg gaagtctctt ttcaggcttt
    56521 ggcagatgcg ataggaaggg atgagatgtc ctaggatgag gagagttccc tgaaggaaaa
    56581 gtggaatatc acttaaagac accaaggaga gaatcatcag gctatcccat aggagaattt
    56641 gtaaagcaaa acgccagaat ctcggtctaa tctgggaata gagttgacta aagtggtcac
    56701 aaagttggtt tgaaagatag gtcaatagca cgggatgaaa gaaaatgagc caaccactat
    56761 aaatcaaaat ccagtcccaa gtaagccccc cttttaaatg tcaaaaatac cagcatgatt
    56821 ccatcaatga caaggagttg aatggttttg atgaagatga tttttttcat gctaaaacct
    56881 ccttttcctt agagacactc ccctacaggt attttagaat gtatattgct tagatattcc
    56941 ttattttatt agctttttaa ggtattaaag tagttatata agtttatgaa gggtatccta
    57001 tcctgcacgg ctaattttga gttcgaaact gaggagttta taactcaaaa tttcgattaa
    57061 atcagcattt aacgatagta tgtttgtcat aaaatgataa atttttagat tgtggaattt
    57121 ttttcaactt aattcttgat tttttttcta aattgttata tacttaattt acgggataac
    57181 agaatcccgt cagaaagggg tgataatatg ggaaaagaga atgttagaat gcgtttagcg
    57241 tctgaatcca gacagatatt agataagctt aaagatgatg tttacaataa attaggctat
    57301 gaagtaagct attccagtat tgttagtcaa gctgttagag aatacgttcc taaaaaagaa
    57361 cgtatagatt ggataaaatt aaaagagaca gctatacctt tttcatcgtt aaaacagagc
    57421 aataattggg agtaccagac atcacttatg ttagagaaag atgtattaat cttactgtct
    57481 gaactacaaa attttttctt agatgtcttc caagcgaaac gtatacatag ggcattttgt
    57541 atacgtctat gtttgagagc gcaattttta ttaagtaata atgattctta atactatcaa
    57601 tattaatgcg gagtgtaatg ctccgcaaaa ataaaaccta ttacgggata aaaacactac
    57661 tttatttagg atggaaataa gaacgtattt tttactaata ttgtttagaa aggagtttct
    57721 ttatgacacc agaagaacaa actgctttgg aatactttta tgaggaactc gagagttacg
    57781 gtgaagatga gcaaggagag gtctttgatt tgtatgataa ttaaatcatc tccatagttt
    57841 ctgagaataa ttgagaaact atactctaaa tcataggttt atgtaaagct agatatacac
    57901 aaaaggaggt acgaatgaag atgaaagcta gagcttcacc gttgaattga ttttgtttct
    57961 agaaaaaatc aataattaga aaaatacgag gtaaaaaaat gaatagaaat gtactaaatt
    58021 ttctgcgaac agaaagtgct gaaagagtta gtctttatat agacaaggct aatagattgg
    58081 agggcgatgt aactctactc gcacctagtt ctcaagattt ggaagatatt aaaaatgcta
    58141 tgttttccaa tcctaatctc gagctaaaag tagcgagact ggatgtgatg aagaaaatag
    58201 catatgcttc tacaagaaat cattatttaa caggggctac gatttttggg gatatttcga
    58261 aaggaacata caattgtgat cctaaaagtt atgtctaata taagggaggt taatccctcc
    58321 ccttgttttt gaaaatcgaa aggaaaagta taaaaatgaa agttaatgaa tatttctgga
    58381 gaacgagctg tcttatacta aaaaagaaga aatcgataaa atagtatacg tgttagagat
    58441 gtgtttgtcg taaaagtctg gatagttttc tcttaggtag tcagctgtca tataataaaa
    58501 agtagaatgg caagcagtga tgattggcat aagagatgaa aaacgctgag aactaatgaa
    58561 ggctaagaga acaaaaaata gaaaatcaat ggtgagaacc cctaaatagt agaagcgaat
    58621 ctttttattg atttgaggat aaatctcagc gaattgatgt ttgagtcgga cgaccaagag
    58681 aaatagcagg agtccttgag caaggaagaa aattaaacca gagagcaaga gccactcaaa
    58741 agatgtctcg agtctagtga taaaaaatgc aaatagtagc aaatcaataa ctgcaattgc
    58801 tataaaatgg attctataga gttttttcat gacaagacct ccctctttta tctaacttca
    58861 ttctactcta aaagaatggg agttacaact aaaatgataa aaatagcaga ggggagattc
    58921 tcttaagttg gctagtattc tttatttgag tttccttcta ttatctaact tcttcatcat
    58981 tccagacaaa taaagctccg attgcattga ggatataaaa gatgtattta ccgatattgg
    59041 cgaagtttcc ttgaatacca gcttttgtca gctgaacgaa attgtaaatc aaccaaaagc
    59101 cccactgagt tgttagtttt aatgcattca aagcattggc aatgagggac agtgcaaagg
    59161 caatagttgt tacgtaggca aggagattca tcttgccccc atatccgata tagttggtca
    59221 caaaggcaaa gaggaaggcg atgatggaaa tgatgatggc cgccaatttt acctgttttt
    59281 ggctcatttg gttgggtctg ccttcttgcg aagctttcca tttctttata gcaaaggtat
    59341 aaatgaggaa ggtgacggga taggtaatga tggccgcctt atttccaagg atataatcaa
    59401 tagcaccgga caaaatggta ttaacaatac caaagtaatt ttcccatttg cttaatttcc
    59461 ccgtgaaacg agtggacaac atggaaatcc caacgttggt tacggaaatc aatccaaagg
    59521 gtacaagagc tgtccatggt ccccagtcta caaatttatc gaggcgtgag ttgaggtaac
    59581 cagatgcaat cgcaatccaa cgaccaaagc aaccccgaag aggtcaaact atttagatgt
    59641 agcaaaaatt tttagtgatt ttttcatagg ttaaactacc tttctttttt tcaaatattc
    59701 tcccattttt tttcaaatat tctaccacca aatgaaagta aaataaaatg atagaaataa
    59761 aaccctgaaa ataaaacttt cagggttggt aggggacttg agaaattagt tgattttctc
    59821 gacatagagt tgttttgcaa tgtccatatt cacttgtaaa tcctcgtcgt tactgaggat
    59881 agtgaaggta ttgctgaagc catcaaactg cttaacttgg agctggtcac cgatgtgaag
    59941 tgagtgcttg tccagataat ggagaatgtc aaaactatcg tgcatccgag tcaggcggta
    60001 ggcgccagct tccttgatat cagctagtgg gaggttattg atttcaacga ggagttctcc
    60061 cttggcagga atagttcctc cgtgggggca ggttttaggg aaacctagca gtttatctag
    60121 tctttccacg aacaggtcag agacagtgtg ttccaagacc tcagcttcct cgtgaatctg
    60181 gtcacttgta tagtctaaat gatgaactag aaaaacttca atcaagcggt gcttacgata
    60241 gagctcagag accagtttga gaccgaggtc agtcagtaga tagccacatt ccttgtcctt
    60301 taggatgaga ttttcacttt tcattcgttt gatcatttca gttacggcag ggggagagac
    60361 ttgcatgcga gccgcaattt ccttgttggt aatcttatgc aggtctatgc caatttcata
    60421 aatacatttt agatagtctt ctttgtttgg ggtcattcgt ttcctcttgt ctaatatctt
    60481 tatctagtat atcaaaaaaa gctagatttc tctagctgtg actttttatg ttatacttat
    60541 tgcaagagaa aaaacgagga gatggatatg acgaaaatag ctcttctttc agatattcat
    60601 ggaaatacca ccgccttgga ggctgttttg gcagatgctc ggcagctagg agtggatgaa
    60661 tactggcttt tgggagatat tctcatgcca gggacagggc gtagaaggat tttggacttg
    60721 ttggatcaac taccgattac ggctagagtt ttgggaaact gggaagacag tctttggcat
    60781 ggtgtccgta aggaattgga tagtactcgc cctagtcaac gctatctctt gcgccagtgc
    60841 cagtatgttt tagaggaaat ttccctagaa gaaattgaag tgctccacaa tcaacctctt
    60901 caaattcatc gtcagtttgg ggatttgaca gtgggaatta gccatcatct gcctgataag
    60961 aactgggggc gagagttgat tcatactggc aaacaagagg aatttgaccg cttggtgact
    61021 catcctccct gtgatattgc tgtttatgga cacattcacc agcagttgct tcgttacggg
    61081 actggtgggc aattgattgt caatccgggt tcgattggcc aacctttctt tttggacgct
    61141 cagctacgga aggacttgcg ggcccagtat atgattttgg agtttgatga caagggcctg
    61201 gtagatatgg acttccgacg ggtagattac gatgtggcag ctgaattgca gctggctaaa
    61261 gacctgagac ttccctattt tgaggtttac tatgaaagtc tggtcaatgg gatccaccat
    61321 actcatcatc aggaattttt gagagaattg gctcagaaag agggttgcga tcgggagtta
    61381 gacgactggt tgaaaagtgg taacgattga cattacaact aaaaagggta taataataga
    61441 aaaagaagag tagaggcagg gtagcctcgt aaaaaggaga agaggatgca aattccaagt
    61501 agatttacca ttgcgactca tatgctgata atcattgccc tcgaggggaa gaaaagcaag
    61561 gtgaccagtg attttctggc tgctagtgtc ggggtcaatc ctgtcattat cagaaagatc
    61621 ttgtcccagt tgaagaaggc agagctgatt tcagtagcgc gtggaacggg cggaacagat
    61681 attgtcaagg accttaagga tattagtctt ttagatgttt atcaggcggt cgaatgtctt
    61741 ggtaagacag gtcaactctt cagtttccat gacaatccga atccaaattg ccctgtagga
    61801 gctcatattc atgatgtttt ggatcaaaaa ttggagagaa ttcagttgac tatggaggca
    61861 gaacttggtc aaaccagtct agaaaaagtc gtggccgatg cagagagtca gatgaaggat
    61921 taagagggat aaatgccctc ttttttcttt ggataattat gataaaatgt agctattaaa
    61981 ggagtaagaa atgtatctcg ttataggaat tgtattagcc tttatagtgt cattttggaa
    62041 ggataataga agtttgtgga atccagtatt atttttatta tccctcattt caagttattt
    62101 ttatctatcc tacctttttt ataaaaatgg atacgagaat gttcagttag ctttttatgt
    62161 atttgccttt gtcttacttc cttttttgct ttttctaagt ggcatctttt taatctataa
    62221 tggggtcatt ttgttgaagc gagagggacg ttccaagccc cattatctct caatgctctt
    62281 tgatttttat tgactattag attctattta aaaagactta gaatgcacat gaaagtatgt
    62341 attctaggtc ttttgttttg tatacattta attttcaaga gtattatcta tttttccatg
    62401 cctggtaacg agtatcaatc aataactggg taaggcgacc caaggtttct ctttcttctg
    62461 gagtgaggga ttgagcttgg acaaagagat gatgaatgta ttcaagcgcc tttaaggaag
    62521 ccaagtcatt gatagagcta ataccagcct caagaatagt gccaaagaag aggtcgaagt
    62581 agagctgaag ttcttcgtca gggactgtgg ccacccattc tttaaaggtt gtgtctactt
    62641 gctggctatc actgttggtc ttatccagtt ggacgaagtg cttgtcctca atctgccaac
    62701 taaaggtatc gtgctgggcg atgccaccca gggcagtact ctgaacgatg atttggtgag
    62761 caggaatttc cagcatcata ccgataatgg aaccttgtgg aatgaatatc ttgcttctat
    62821 ccattatcct ttgataaccc gcagtctgtg tcaattcttg atggagacca ggtgcatcaa
    62881 atgtataaac tgctgtgatc tgattttgca aactttgctc aatttggcta gcagcataga
    62941 tagcgagatt tcctcccttg gaatgcccag ctagaataac cttttgctta ggatgatggg
    63001 caaaaaagtt ctttaaatag cgaagggcgt gcttttgagc aggaatttcc ttcatatagg
    63061 tcaggtggaa atcttccttc cagccaatga tactgtcatc tgtcccacga aagacaatca
    63121 gataggtatc gaggctgaca cgataagtca tagccgcaaa ttgcttttgc agttcagggt
    63181 cgatgtcgtt gataaaatgg gagagtttgc aatttttgaa gcgcttgtgt tgagccaatt
    63241 tatctaataa ttgaaggcga tttttgctag taagcatggt gggctctctt ggaacctgag
    63301 gtgctagatc taaaagacgt tgaggaagtg tggagaccag attatcaaag gagaggtagg
    63361 tgatttctat taaggttaga atgtctaact cattcaaggg aaggtcgtaa taagaatcat
    63421 atgcgacatc tttcagatag tcaaaaatat tggccataag agctcctttc tttttattta
    63481 tcttagcaaa tttccctcaa attagctagt agcatagcct gtttgcactg gctaaaaaca
    63541 ggctatttca aattcagttt cagaccatct agcatggaaa aatctgttat aataatggaa
    63601 aaggagaagc gcatgcacaa gattttatta atagaagatg atcaggtcat tcgtcaacag
    63661 attgggaaaa tgctctctga atggggattt gaagtggtcc tggtagaaga ctttatggaa
    63721 gttttgagtc tatttgttca gtcggaacct catctggtcc tcatggatat tggtttgccc
    63781 ttgtttaatg gttatcactg gtgtcaggaa atccgcaaga tttccaaggt acctatcatg
    63841 tttctttctt cgagagacca ggctatggat attgtcatgg caatcaatat gggggcggat
    63901 gactttgtga ccaagccttt tgaccagcag gttcttttag ctaaggttca gggcttgttg
    63961 cgtcgttcct atgagtttgg gcgtgatgag agtttgctgg aatatgctgg tgttatcctc
    64021 aataccaaat ccatggattt acattatcaa gggcaagtct tgaatttgac caagaatgaa
    64081 ttccagattt tacgcgtgtt atttgagcat gcaggcaaca tcgtagcacg tgacgacctg
    64141 atgcgggaac tttggaacag tgactttttc attgatgata ataccctctc tgtcaatgtg
    64201 gctcgtttgc gtaaaaagtt ggaggagcag ggattggtag gatttatcga gaccaagaaa
    64261 ggaatagggt acggattgaa gcatgcttga ttggaaacaa ttttttctag cctatctgcg
    64321 ctcccgtagt cgtcttttta tctatctgct ttctttggca tttcttgtct tactctttca
    64381 gtttttattt gccagtctag gaatttactt cctctacttt ttcttcttgt gttgctttgt
    64441 aaccatctta tttttcactt gggacatatt ggtggaaatg caggtctatc gccaggaact
    64501 tctctatgga gagagggaag ccaagtctcc tttggaaata gctttagcag aaaaattaga
    64561 agcgcgtgag atggaactct atcagcagag gtcaaaagca gaaagaaaac tgacggattt
    64621 gctggattac tataccttgt gggtccatca gataaagacc cccattgcag ccagtcaact
    64681 cttagttgca gaagtggtcg accgccaact gaagcagcag ctagaacagg aaattttcaa
    64741 aatcgactct tataccaacc tagttttaca gtacctgcgt ttagaaagtt tccatgatga
    64801 tttggtctta aagcaggttc aaattgagga cttggtcaag gaaataattc gtaaatatgc
    64861 tcttttcttt attcaaaaag gcttaaatgt caatctacat gaccttgata aagaaatcgt
    64921 gacggataaa aagtggctgc tagtggttat tgagcaaatc atctcaaaca gtctcaagta
    64981 caccaaggaa ggtggtctgg agatttatat ggatgaccaa gagctttgta tcaaagatac
    65041 gggaatcggg ataaaaaaca gtgatgtcct ccgagtattt gaacgtggct tttcaggata
    65101 caatggccgt ttgacccagc agtcctctgg acttggcctt tatctatcta agaaaatttc
    65161 tgaagaactg gggcaccaga ttcgtatcga gtctgaggtc ggaaaaggaa cgacagtgcg
    65221 gattcagttt gctcaagtga acttagtcct tgagtaaaaa tagatgagaa aaagcctcca
    65281 gattggaggc ttttatgcta gactcttatt tctcaacgcg tgatttgttg gcgatatcag
    65341 ctaggatagc ttgaacaaaa ttatcaactg agacagtttg tgtttctttt tggccgtagc
    65401 gacgaacgtt gactgtttcg tcttccattt ctttgtctcc aacaattaat tggtaaggaa
    65461 tcttgctggt ttgtgaagca cggatcttga actgcatttt ttcattgcgc tcatctacgt
    65521 ctgcacggac accgcggtca cggagtttct tggctacttc ccaagcgtag tccacgtgtt
    65581 tttcgttaga tactgggata agggttactt ggtgtggtgc cagccatgtt gggaaggcac
    65641 ccttgtagtt ctcaatcaag atagctgtga agcgttccat agttgagata accccacggt
    65701 ggatcatgac tggacggtga tcttcgccat cagctccgat gtatttgagg tcgaagcgtt
    65761 ctggcaacaa gaaatcaagt tggatagtag aaagggtttc ttcttttcca agggcagttt
    65821 taatctggat atccaatttt ggtccgtaga aggccgcttc accttcggct tcaaagtagt
    65881 ccacgcccat ttcatcaaga gctgcacgaa gcatggtttg ggcattttcc cacatctcat
    65941 cgttatcaaa gtacttatga gtatcttgag ggtcacgaag agagaggcgg aagcggtagt
    66001 cagtcaagtt gaagtcttca taaacatcga taatcaactg aagggcacgt tggaattctt
    66061 cttggatttg ttctggagta acgaataggt gaccgtcgtt gagtgacatt tcacgtacac
    66121 gttgaaggcc agtgagggca ccagattttt cgtaacggtg catcataccg atttcagcga
    66181 tacggattgg caattcacgg taagagtgaa catggtgttt gaaaacttgg atgtggtgcg
    66241 gacagttcat tggacgaagg acaaattctt ccccgtcacc catgtccatg gttgggaaca
    66301 tgtcttcttg gtaatgatcc cagtggccag aagtcttgta aagctcaaca gaagcaagtg
    66361 gtggagtgta gacgtgttgg tagccagaag ccaactcttt gtttacgatg tagcgttcca
    66421 attcacgacg gatagtcgca ccatttggca accagaatgg caaaccttgt cccacttctt
    66481 gtgaaatcat aaagaggtca agctctttac caagtttacg gtggtcacgt tccttagctt
    66541 cttcacgcat ttgaaggtag tttttcaagt ctttcttttc aaaccaagct gtaccgtaga
    66601 tacgttgcat catagcgttg tcgctgtttc cacgccagta cgcaccagct acatggagaa
    66661 ggtggaagat ttggatacga cctgttgatg gaacgtgagg tccacggcag aggtctacat
    66721 attcaccctg acgatagata gtcaaaccgc cttcgtcttc tgagtgttct tcaatcaatt
    66781 ccaacttgta agggtcattt ttgaagattt cacgtgcctc gtctttagtc acttcttcac
    66841 gaatagatgg gaagttttct ttgacgattt tttgcatttc ttcttcgata cgaggaaggt
    66901 cttcgttaga gatttgacca gctgtgttgt cagtatcgta gtagaaacca tcttcgatgg
    66961 ctggaccaac tcccaagtga atgtctggga aaagacgacg agctgcttgg gcgaacaagt
    67021 gagctgctga gtggcgcaag attggaaggg catcttcgtg atcaggtgtc acaatttcga
    67081 tgcttccatc ttcagtgata gcgcgagtag tgtcgatgag tttgccgttg aatttaccag
    67141 ccaaggcttt tttagctagg gaattgctga tagattgggc aatttcaaaa gttgttacgc
    67201 cagattcgaa ttcacgaaca gcgccatctg ggaaagtaat gttaatcatg atgttctcct
    67261 tttttattat ttcatagttt ctaagcattg attttttatc atcagactac ttccaagtcc
    67321 taacgttcaa ttgcttttct ttatcagaga ggaagttttc catttccgtc agaatgtcta
    67381 tatccaaacg ttgagaaagt tctaatagcc accagatatt ttctgaaagt ttttgttcca
    67441 gtgtgtaggg tgtttcatca tagtagcgtc cttgctttgt catcaccagt cgttggaaat
    67501 ttccaatatc attagataaa gccaaggggt cttcttctac cgtccacttg gaatcatgat
    67561 gcttaacttc cagttcgtga taagcttgtc ggattgccca actgcgctcc actaattctt
    67621 gcaactccat aattagtatc ctaataaaaa aatgcgacat cctcgaaagg acgtcgcaac
    67681 gtggttccac cttcatttat gtacctcaaa agagagggac acctctgatt ggctctaacg
    67741 tggccaccgt tttatgtttt cataaaaaac tcaagagtag tatcagttta gacttcctat
    67801 gcgtttccag caaccacgca ctctctagta gaaagggact aagtgacttg tctctatgga
    67861 ttattatagc atgattgtga gatttttcaa ggattttgtg aaagtttttt gtttttcatt
    67921 ttttgtagaa tatgcttgat gctaatacca gaatccatgc ccaccagagc aactgtttca
    67981 tagattagca agtaaattag tataaactca ccaaaggtta tgatagtaaa gtaaaagagc
    68041 agtccgttac caactaacca tcaaagtgag aaacggaaac ggtagctata gagtaacgat
    68101 acaatcaaga ttaccagagg tgtaaagaag agaagattgt ttatatagag ttccttgagt
    68161 gtaagggggt cagaaacaac tagtaagaac aactcataaa gtggccaata aaagaataat
    68221 atgaccagat agtaggggag atgggaatag aatctacgca tgataatcac ctcgttttaa
    68281 aaaaacagac aacttagatc tcttgctatg attattatag catgtttaaa acaagaaagt
    68341 aaatttgttg gcaaagaaga tttttttttc actagaatca tggttagttt tcttggtaga
    68401 aatattttga taataattaa gcaaaactat tgacattgat tattaagagt gtcacaattg
    68461 ttgtaactaa aataattaca ataagagagg tataaaaaat gaaactagca gttattgcag
    68521 caaatggaca agcggggaaa gcaattgttg aagaagtagt caaacgagga catgaagtaa
    68581 cggctattgt tcgttctgaa aacaaaagtc aatctgagtc tattattaaa aaagatttat
    68641 ttgagttgac aaaagatgat cttactggat ttgatgctgt tatttcagcg tttggtgctt
    68701 atacaccaga tacgctaccg ctccacagca aatcaatcga actatttaat cagcttcttg
    68761 ctggaacaca gacacgtttt ctagttgtag ggggagcagg aagtctttat atagatgaaa
    68821 caaaaacgac acgactttta gatacgtctg attttcctga agaatttaag tcactagcaa
    68881 aagctcaagc ggatgaattg gatcttcttc gtacaaaaaa taatctcaac tggacttttg
    68941 tcagtcctgc tgtagatttt attcccgatg gtgaaaaaac aggaaactac attctagcag
    69001 gagaaatttt tactactaat gaaaaaggaa taagccagat tagttatgca gattatgcta
    69061 ttggattggt agatgaactt gaaaaaggtc atcatataaa agaacgtatc tctttacttg
    69121 aaaaataata actatgagag tattaatgtt tgacagaaaa tttaaatctt ggtaaaatag
    69181 ggattgtcgt aaagacaaat aacttcttct tggttacagg catgccaacc tgtcactcgg
    69241 atgaagccaa ataaaaagga gaaacatcat ggcaatctca aaagagaaaa aaaatgaaat
    69301 cattgcacaa tatgcacgtc acgaaggtga tacaggttca gtagaggttc aagttgctgt
    69361 ccttacttgg gaaatcaacc accttaacga acacatcaaa caacacaaaa aagaccacgc
    69421 tacttaccgt ggattgatga aaaaaatcgg tcgccgtcgt aacttgcttg catacttgcg
    69481 taaaaacgac gttaaccgtt accgtgagtt gatcaactct ctaggacttc gtcgctaatt
    69541 caagatacaa aggccgtcaa aaacacaaag caaaaatagg aaaattgacg aagaaacttc
    69601 agtttctagg agattttatc ttttttgcca agtgcttagg ccgtgttcaa ttgagcatat
    69661 cttgataata aagctactct aaattgggta gctttgttgc gttgtcacct tacctcgcta
    69721 tactcaagta taatcttcgg ttacggttcc tagcactgta aggtaaaata aactagcttt
    69781 tctcccttcg gggagatttt tttgtttcac tgaaagtttc gtacgatctt cacctcgtcc
    69841 cctagtctat aaacgattta tctagcaaga atcatgatgc taagggcgtc aaaaatccgt
    69901 atgaaaatag ggaaatgaca cagtgttcga tgagcacaag gagtttcatc tttttcacta
    69961 ggattttagc ccgggctaaa atcagctctc tgacttcaga gggctttttt attgaggttt
    70021 tatcggtaac aattttggag actacaaaaa cctcaaatgg tatcagctaa ttacaccata
    70081 aaggtgcgca gttactcggc ttttcaagcc gagtagctgt atgcaagccc cctagaagag
    70141 cccactcttt acaaagtaaa gtatagtatg ttatacttta catggaagta gtcactgaat
    70201 tccagttaga aattactttg taactacgtt ttgaggagga gtaaaatgct ttcctacgtt
    70261 cgacattacc cactagcgat agctaaatta atgtgtctgt gctctcctaa aatctgctga
    70321 tttattactg actaatacag gaggtttttt ttatgggaca gacaatcata tctgctattg
    70381 gtgtttatat ttccaccagt atcgattatt taattatttt aattatttta tttgcacagc
    70441 tatcacagaa taaacagaaa tggcatattt atgcggggca atatctaggc acaggcttac
    70501 ttgtaggggc gagtttagtt gctgcttatg tcgttaattt cgtgcctgaa gaatggatgg
    70561 ttggattgct tggtttaatc cctatctatt tagggattcg ctttgcaatt gttggagaag
    70621 atgcggaaga agaagaggaa gaaattattg aaagattaga acaaagcaag gcaaatcaac
    70681 tgttttggac agttacattg ctgacaattg cgtctggcgg agataattta ggtatctata
    70741 taccttattt tgcttcgtta gattggtcac agaccctcgt ggccttgctt gtgtttgtaa
    70801 tcggcataat tatcttttgc gagattagtc gggtgttatc ctctattccg ttaatattcg
    70861 agacaattga aaaatacgag cgaatcattg tgcccttagt attcattcta cttggactat
    70921 acatcatgta tgaaaatggc acgatagaga cttttctgat cgtgtagatt tttttgtttc
    70981 actaggattt tagcccgagc tcaaatcagc tctctgattt tcagagggct ttttcattga
    71041 gtataaatgg acaatcttaa agttactcta ttgtagagtg gcttttgatt tgattaggag
    71101 attcttataa atatagtaaa atgaaacaaa aacaggacaa atcgatcagg acagtcaaat
    71161 cgatttctaa aaatgtttta agaagtagag atgtactatt ctagtttcaa tttgatatat
    71221 aaatgaaaac cttagctgtt tggccaaggt tcatttcttc tatgcaagtt cttctctttt
    71281 cttatctggg ttccagatga agcttgcgat aaagctaaag atgatggtta ggatagcgag
    71341 gataatagca aggatgaggg cagggatgcg gataaaccac cagagtggat ttggtttttt
    71401 atcattgtgc cattgtttcg tttcttcgtc cagacgttgg aattctgttt gaatacgatt
    71461 ggcgactgtt tcaatgcctt catcattcat tttcttgata tctgagatat cgattggatt
    71521 tccaaagttc atatcgacac gttcacggct aatcaagccc ttcaaagtca tgggaccggt
    71581 gtaggtaacc ggcatgatac ggaccttggc cattttggca atcagtgctg cgcccccctt
    71641 gacatcgttt gagtggcggc taccacttgg aaacatgatg agagagcggt cacttttttt
    71701 gagaacgttg ataggatatt tgatggctga ggcgctggga ttttcacggt cgatgggaaa
    71761 ggcgccacac atacgaatcc accaaccaaa gatacggttg gtaaagagtt ctttttttgc
    71821 cataaagatg aactgttttg gcttggtcgc aaaggccata taaacaggat cccaccaggt
    71881 acggtgaggc gcaactaaaa tataattttc atcttgatta ggaattttat cagtattatg
    71941 atagtgagca ttgccattga tggaccataa gagcaataca actaatccac gcaaataagt
    72001 ataaaacatg cgatctcctt cgattgtttt cttgttatta ttatacctta tcaaaggagg
    72061 gctggcaaac ttttcccttg actagatgca tatttgggat gaaattagaa ttctgttaaa
    72121 aaaaatgata taatagaatt tatggataaa aataagatta tgggattaac ccaaagagaa
    72181 gtcaaggaaa gacaggctga gggtttggtc aatgacttta ccgcatcagc cagtaccagc
    72241 acttggcaaa tcgttaaacg aaatgtcttt acccttttta acgctttgaa ctttgccatt
    72301 gctttggccc ttgcctttgt gcaggcttgg agcaatctgg tcttctttgc tgttatctgc
    72361 tttaacgctt tttctgggat tgtgaccgag ctacgagcca aacacatggt ggacaagctc
    72421 aatctcatga ccaaggaaaa ggtcaaaacc atccgtgatg gtcaggaagt tgctcttaat
    72481 cctgaagaat tagtgctagg agatgtcatt cgtttgtctg caggagagca gattcctagt
    72541 gatgccttgg ttttggaagg ctttgcggaa gtcaatgaag ccatgttaac gggagaaagt
    72601 gatttggtgc aaaaggaagt tgacggctta cttttgtcag gaagtttcct agccagtggg
    72661 tcagttttat ctcaagttca ccatgtcggt gcagacaact atgctgccaa actcatgctt
    72721 gaggctaaga ccgttaaacc catcaactcc cgtatcatga aatcgctgga caagttggct
    72781 ggttttactg ggaagattat cattcccttt ggtctggctc tcttgctgga agccttgctt
    72841 ttaaaaggcc tgcctctcaa gtcatccgtt gtaaactcgt cgacagctct tttgggaatg
    72901 ttgcctaagg gaattgccct tttgaccatt acttcgctct tgactgcagt gattaagttg
    72961 ggcttgaaaa aggtcttggt gcaggagatg tactctgttg agaccttggc gcgcgtggat
    73021 atgctctgtc tggacaagac gggcaccatc acccaaggaa agatgcaggt ggaggctgtt
    73081 cttccactga cggaaactta cggtgaagag gctattgcca gcatcttgac tagctacatg
    73141 gcccatagtg aggataagaa tccaactgcc caagccattc gccagcgttt tgtgggagat
    73201 gttgcttatc ctatgatttc caatcttccc ttctccagcg accgcaagtg gggggctatg
    73261 gagttagaag gcttggggac agttttctta ggggcacctg agatgttgct ggattctgaa
    73321 gtcccagaag ccagggaggc cttggagaga ggatcacgtg tcttggtctt agctctcagt
    73381 caggagaaat tagaccatca caaaccacag aaaccatctg atattcaggc tctagccttg
    73441 ctggaaatct tggaccccat tcgagaggga gcagcagaga cgctggacta tctccgttct
    73501 caggaggtgg gactcaagat tatctctggt gacaatccag ttacggtgtc cagcattgcc
    73561 cagaaggctg gttttgcgga ctatcacagc tatgtagatt gctcaaaaat caccgatgag
    73621 gaattgatgg ccatggcgga ggagacagct attttcggac gtgtttcccc tcatcaaaag
    73681 aaactcatca tccaaacgtt gaaaaaagcg ggtcatacaa cggctatgac aggggacggg
    73741 gttaatgata tcttggccct tcgtgaggcg gattgttcta tcgtgatggc ggagggagat
    73801 ccagcaaccc gtcagattgc caatctggtt ctcttgaact cagactttaa tgatgttcct
    73861 gagattctct tcgagggtcg tcgcgtggtc aataacattg cccacatcgc cccgattttc
    73921 ttgataaaga ccatctattc cttcctgtta gcagtcatct gtattgccag tgctttacta
    73981 ggtcggtcag agtggatttt gattttcccc ttcattccga tccagattac catgattgac
    74041 cagtttgtgg aaggtttccc accattcgtt ttgacttttg agcgaaatat caaacctgtc
    74101 gagccaaact tcctcagaag atccatgctt cgtgccctac caagtgccct catggtcgtg
    74161 tttagcgttc tttttgtgaa aatctttgga acgagtcaag gttggtctga gttagaaatc
    74221 tcaactctcc tctattatct cttggggtca attggtttct tatccgtatt tagagcctgc
    74281 atgccattca ctctatggcg agtcctcttg attgtctggt cagtaggagg cttcctagcc
    74341 acagctctct tcccaagaat ccaaaaactg cttgaaattt caaccttgac agaacaaacg
    74401 ttgcctgttt atggtgtcat gatgttggtc tttacagtga ttttcatcct gactagtcgc
    74461 tatcaagcta gaaaataaag aaagactgca atctgtggat tgcagttttt ttaggtacaa
    74521 gattgctagc cgaaatgtgg tataataaga ggtaatagag ttttggaaag tgagagaaga
    74581 tgatttcaaa gagattagaa ttggtagctt cctttgtgtc acagggggct attttactag
    74641 atgtgggaag tgaccatgct tatctgccta tcgagttggt tgagagaggc caaatcaaaa
    74701 gcgctattgc aggtgaggtg gtggaaggtc cctatcagtc tgcggttaaa aatgttgagg
    74761 ctcacggcct aaaggagaaa atccaagtcc gtttagccaa tggcttggca gcttttgaag
    74821 agactgacca agtgtctgtc attaccattg ctggcatggg tggtcgtttg attgctagga
    74881 ttttagaaga aggtttgggg aagttagcta atgtagagcg tttgatcctc cagcccaata
    74941 atcgtgaaga cgacttgcgt atctggctac aggatcatgg attccagatt gtagcagaaa
    75001 gcattttaga agaagctgga aagttttatg agattttggt ggtggaagca ggacaaatga
    75061 agctatcagc cagtgatgtt cgctttggtc ccttcttgtc caaagaagtc agtccagtat
    75121 ttgtccaaaa atggcaaaaa gaagctgaga agctagagtt cgccctcgga caaatcccag
    75181 aaaaaaatct ggaagaacgt caagttctag tagataagat tcaagctatc aaggaggtgc
    75241 tccatgttag caagtgaagt gatccaagct tatgaagcct tttgccctca ggaattttct
    75301 atggagggag acagccgtgg tctgcaaatt ggcactttag acaagggtat ccaaagggtc
    75361 atggttgccc tcgatattcg tgaagagacg gtggcagagg ctattgaaaa gggtgtggac
    75421 ttgattatcg tcaagcacgc gcctatcttt cgtcctatca aggacttgct tgccagccgt
    75481 ccgcaaaatc agatttacat cgacctgatt aagcatgata tcgcagttta tgtcagccat
    75541 accaatattg atatcgttga aaatgggctc aatgactggt tttgtcagat gctaggaatc
    75601 gaggagacga cttatctgca ggaaacaggt ccagaacgtg gaattggacg tattgggaat
    75661 attcagcctc agacattttg ggaattggcc caacaggtca agcaagtctt tgacctagat
    75721 agccttcgaa tggtgcatta tcaagagaat gatttgcaga agcctatttc aagagtagca
    75781 atttgtggtg gaagcgggca gtctttctat aaggatgctt tggcaaaggg ggcagatgtc
    75841 tatatcactg gtgatatcta ctaccatact gctcaggata tgctgtctga tggcttgtta
    75901 gcattggatc caggtcacta tatcgaagtg ctttttgtgg aaaaaatcgc agcactcctt
    75961 actcaatgga aagagaaaaa aggctgggaa ctagagattt tacctagtca agcatcgacc
    76021 aatcctttcc gtcatatcta gttagaaagt gaagacaatg aaaaaagttg ccattattgg
    76081 agcaggaatt gtgggagcaa cagctgccta ctacctctcg agagaaagtg acctagaggt
    76141 gaccgttttt gaccatgagc aaggtcaagc caccaaggcc gcagcaggaa ttatcagtcc
    76201 ttggttttcc aaacgccgta ataaagcctg gtacaagatg gcgcgcttgg gggctgattt
    76261 ttatgtggat ttattagctg atttagagaa atcaggacaa gaaatcgact tttaccagcg
    76321 ttcgggagtc tttctcttga aaaaggatga atccaagttg gaagaactct atcaactggc
    76381 tctccagcgc agagaagaat ctcccctgat aggccagtta gccattctgg atcaagcatc
    76441 agctaatgaa ttattccctg gcttgcaggg attcgacaac ttgctctatg cttctggtgg
    76501 agcgagagta gatggccaac tcttggtaac tcgtctgcta gaagccagtc atgtcaagtt
    76561 ggtcaaagaa aaagtgactc tgacactctt atcatcaggc taccagattg gcgaagaggt
    76621 gtttgatcag gttattttgg caacgggagc ttggttgggg gacatgttag aacctttagg
    76681 ttatgaagtg gatgtccgtc ctcaaaaagg acaactacga gattatcagc ttgcccaaga
    76741 catggaagat taccctgttg tcatgccaga aggggagtgg gatttgattc cctttgcagg
    76801 tgggaaatta tccttgggcg ctacccatga aaatgacatg ggatttgatt tgacggtaga
    76861 tgaacccttg ctccaacaaa tggaggaggc ggccttgcct cattacccag ttttagctag
    76921 agtgacttca agggctgagc gtgtgggaat ccgtgcctat accagtgatt tctctccctt
    76981 ctttgggcag gtgccagaat tggcaggtgt ctatgcagcc agtggactag gttcatcagg
    77041 cctcacaact ggtcctatca ttggttacca tctagcccaa ctgatccaag acaaggagtt
    77101 gaccttggac cctctaaatt acccaattga aaactatgtc aaacgagtaa aaagcgaata
    77161 atactcaatg aaaatcaaag aacaaactag gtaactagcc gcgggttact caaagcactg
//
LOCUS       DBEJAQ010000004        67422 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.004, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000004 DBEJAQ010000000
VERSION     DBEJAQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 67422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.004"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..383
                     /locus_tag="ACL297_000298"
     CDS             <1..383
                     /locus_tag="ACL297_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HGR0070625.1"
                     /translation="ATGFYYGTPSENEQLFGARRKIPEYKFVAEVVSYDDATQTATIR
                     QRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIKVPQLVQAGD
                     MVRALKEGLINLYKEDGTSVTVRA"
     gene            740..1611
                     /locus_tag="ACL297_000299"
                     /pseudo
     CDS             740..1611
                     /locus_tag="ACL297_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1636..2106
                     /locus_tag="ACL297_000300"
                     /pseudo
     CDS             1636..2106
                     /locus_tag="ACL297_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2492..2701
                     /locus_tag="ACL297_000301"
     CDS             2492..2701
                     /locus_tag="ACL297_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0070626.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2691..3179
                     /locus_tag="ACL297_000302"
     CDS             2691..3179
                     /locus_tag="ACL297_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HGR0070627.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            3295..4755
                     /locus_tag="ACL297_000303"
     CDS             3295..4755
                     /locus_tag="ACL297_000303"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGR0070628.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            4752..5576
                     /gene="nadE"
                     /locus_tag="ACL297_000304"
     CDS             4752..5576
                     /gene="nadE"
                     /locus_tag="ACL297_000304"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGR0070629.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5926..6477
                     /locus_tag="ACL297_000305"
     CDS             5926..6477
                     /locus_tag="ACL297_000305"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070630.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            7522..7884
                     /locus_tag="ACL297_000306"
     CDS             7522..7884
                     /locus_tag="ACL297_000306"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGR0070631.1"
                     /translation="MKVPAKSKVEEVFVPFATKYEADNDLSAGQEQEITLGKNGKTVT
                     TITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEISIETEYLDGPTLDKSQ
                     EVEEVGEIGKLLLLQSIL"
     gene            7938..8327
                     /locus_tag="ACL297_000307"
     CDS             7938..8327
                     /locus_tag="ACL297_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGR0070632.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(8988..10016)
                     /gene="queA"
                     /locus_tag="ACL297_000308"
     CDS             complement(8988..10016)
                     /gene="queA"
                     /locus_tag="ACL297_000308"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGR0070633.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFY
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            10167..10874
                     /locus_tag="ACL297_000309"
     CDS             10167..10874
                     /locus_tag="ACL297_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGR0070634.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            11014..11190
                     /gene="rpsU"
                     /locus_tag="ACL297_000310"
     CDS             11014..11190
                     /gene="rpsU"
                     /locus_tag="ACL297_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGR0070635.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            11320..12255
                     /gene="hprK"
                     /locus_tag="ACL297_000311"
     CDS             11320..12255
                     /gene="hprK"
                     /locus_tag="ACL297_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGR0070636.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            12248..13036
                     /gene="lgt"
                     /locus_tag="ACL297_000312"
     CDS             12248..13036
                     /gene="lgt"
                     /locus_tag="ACL297_000312"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGR0070637.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEK"
     gene            13037..13463
                     /locus_tag="ACL297_000313"
                     /pseudo
     CDS             13037..13463
                     /locus_tag="ACL297_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            13436..13825
                     /locus_tag="ACL297_000314"
     CDS             13436..13825
                     /locus_tag="ACL297_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGR0070638.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            13909..15039
                     /gene="hemW"
                     /locus_tag="ACL297_000315"
     CDS             13909..15039
                     /gene="hemW"
                     /locus_tag="ACL297_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGR0070639.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            15044..15781
                     /locus_tag="ACL297_000316"
     CDS             15044..15781
                     /locus_tag="ACL297_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HGR0070640.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            15792..16565
                     /locus_tag="ACL297_000317"
     CDS             15792..16565
                     /locus_tag="ACL297_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HGR0070641.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            16555..17172
                     /locus_tag="ACL297_000318"
     CDS             16555..17172
                     /locus_tag="ACL297_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGR0070642.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            17382..17783
                     /locus_tag="ACL297_000319"
     CDS             17382..17783
                     /locus_tag="ACL297_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HGR0070643.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            17783..18061
                     /locus_tag="ACL297_000320"
     CDS             17783..18061
                     /locus_tag="ACL297_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HGR0070644.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            18051..18824
                     /locus_tag="ACL297_000321"
     CDS             18051..18824
                     /locus_tag="ACL297_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HGR0070645.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            18825..20129
                     /locus_tag="ACL297_000322"
     CDS             18825..20129
                     /locus_tag="ACL297_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGR0070646.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            20703..21581
                     /gene="pstS"
                     /locus_tag="ACL297_000323"
     CDS             20703..21581
                     /gene="pstS"
                     /locus_tag="ACL297_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGR0070647.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            21581..22498
                     /gene="pstC"
                     /locus_tag="ACL297_000324"
     CDS             21581..22498
                     /gene="pstC"
                     /locus_tag="ACL297_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGR0070648.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            22488..23372
                     /gene="pstA"
                     /locus_tag="ACL297_000325"
     CDS             22488..23372
                     /gene="pstA"
                     /locus_tag="ACL297_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGR0070649.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            23383..24186
                     /gene="pstB"
                     /locus_tag="ACL297_000326"
     CDS             23383..24186
                     /gene="pstB"
                     /locus_tag="ACL297_000326"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGR0070650.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            24199..24957
                     /gene="pstB"
                     /locus_tag="ACL297_000327"
     CDS             24199..24957
                     /gene="pstB"
                     /locus_tag="ACL297_000327"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGR0070651.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            24969..25622
                     /gene="phoU"
                     /locus_tag="ACL297_000328"
     CDS             24969..25622
                     /gene="phoU"
                     /locus_tag="ACL297_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGR0070652.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            25779..26594
                     /locus_tag="ACL297_000329"
     CDS             25779..26594
                     /locus_tag="ACL297_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0070653.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(26614..27885)
                     /locus_tag="ACL297_000330"
     CDS             complement(26614..27885)
                     /locus_tag="ACL297_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGR0070654.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            28027..28758
                     /gene="budA"
                     /locus_tag="ACL297_000331"
     CDS             28027..28758
                     /gene="budA"
                     /locus_tag="ACL297_000331"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HGR0070655.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            28768..29520
                     /locus_tag="ACL297_000332"
     CDS             28768..29520
                     /locus_tag="ACL297_000332"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HGR0070656.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            29632..30537
                     /gene="murB"
                     /locus_tag="ACL297_000333"
     CDS             29632..30537
                     /gene="murB"
                     /locus_tag="ACL297_000333"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGR0070657.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            30697..31854
                     /locus_tag="ACL297_000334"
     CDS             30697..31854
                     /locus_tag="ACL297_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070658.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            31835..32641
                     /locus_tag="ACL297_000335"
     CDS             31835..32641
                     /locus_tag="ACL297_000335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0070659.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            32638..33411
                     /locus_tag="ACL297_000336"
     CDS             32638..33411
                     /locus_tag="ACL297_000336"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0070660.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            33408..34478
                     /locus_tag="ACL297_000337"
     CDS             33408..34478
                     /locus_tag="ACL297_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0070661.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            34739..34978
                     /locus_tag="ACL297_000338"
     CDS             34739..34978
                     /locus_tag="ACL297_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070662.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            35253..35738
                     /locus_tag="ACL297_000339"
     CDS             35253..35738
                     /locus_tag="ACL297_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HGR0070663.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            35760..38378
                     /gene="alaS"
                     /locus_tag="ACL297_000340"
     CDS             35760..38378
                     /gene="alaS"
                     /locus_tag="ACL297_000340"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGR0070664.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPCITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(38468..39922)
                     /locus_tag="ACL297_000341"
     CDS             complement(38468..39922)
                     /locus_tag="ACL297_000341"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGR0070665.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            40101..40850
                     /locus_tag="ACL297_000342"
     CDS             40101..40850
                     /locus_tag="ACL297_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070666.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            40831..42465
                     /locus_tag="ACL297_000343"
     CDS             40831..42465
                     /locus_tag="ACL297_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070667.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            43150..44313
                     /locus_tag="ACL297_000344"
     CDS             43150..44313
                     /locus_tag="ACL297_000344"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HGR0070668.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            44310..44987
                     /gene="aroD"
                     /locus_tag="ACL297_000345"
     CDS             44310..44987
                     /gene="aroD"
                     /locus_tag="ACL297_000345"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGR0070669.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            44977..45831
                     /locus_tag="ACL297_000346"
     CDS             44977..45831
                     /locus_tag="ACL297_000346"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGR0070670.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALENVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            45850..46917
                     /gene="aroB"
                     /locus_tag="ACL297_000347"
     CDS             45850..46917
                     /gene="aroB"
                     /locus_tag="ACL297_000347"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGR0070671.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKTRGNTLKLVLVS
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            46927..48093
                     /gene="aroC"
                     /locus_tag="ACL297_000348"
     CDS             46927..48093
                     /gene="aroC"
                     /locus_tag="ACL297_000348"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGR0070672.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            48103..49206
                     /locus_tag="ACL297_000349"
     CDS             48103..49206
                     /locus_tag="ACL297_000349"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGR0070673.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            49217..49555
                     /locus_tag="ACL297_000350"
     CDS             49217..49555
                     /locus_tag="ACL297_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HGR0070674.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            49647..50930
                     /gene="aroA"
                     /locus_tag="ACL297_000351"
     CDS             49647..50930
                     /gene="aroA"
                     /locus_tag="ACL297_000351"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGR0070675.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            50923..51399
                     /locus_tag="ACL297_000352"
     CDS             50923..51399
                     /locus_tag="ACL297_000352"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGR0070676.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            51396..52244
                     /gene="pheA"
                     /locus_tag="ACL297_000353"
     CDS             51396..52244
                     /gene="pheA"
                     /locus_tag="ACL297_000353"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGR0070677.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            52241..53515
                     /gene="psr"
                     /locus_tag="ACL297_000354"
     CDS             52241..53515
                     /gene="psr"
                     /locus_tag="ACL297_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HGR0070678.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            53794..54639
                     /locus_tag="ACL297_000355"
     CDS             53794..54639
                     /locus_tag="ACL297_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGR0070679.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            54629..55732
                     /locus_tag="ACL297_000356"
     CDS             54629..55732
                     /locus_tag="ACL297_000356"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGR0070680.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            55734..56720
                     /locus_tag="ACL297_000357"
     CDS             55734..56720
                     /locus_tag="ACL297_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGR0070681.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            56736..58169
                     /locus_tag="ACL297_000358"
     CDS             56736..58169
                     /locus_tag="ACL297_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HGR0070682.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            58198..59232
                     /locus_tag="ACL297_000359"
     CDS             58198..59232
                     /locus_tag="ACL297_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGR0070683.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            59432..60169
                     /gene="mecA"
                     /locus_tag="ACL297_000360"
     CDS             59432..60169
                     /gene="mecA"
                     /locus_tag="ACL297_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HGR0070684.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            60320..61606
                     /locus_tag="ACL297_000361"
     CDS             60320..61606
                     /locus_tag="ACL297_000361"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGR0070685.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            61608..62477
                     /gene="thrB"
                     /locus_tag="ACL297_000362"
     CDS             61608..62477
                     /gene="thrB"
                     /locus_tag="ACL297_000362"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGR0070686.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            62772..63710
                     /gene="msrB"
                     /locus_tag="ACL297_000363"
     CDS             62772..63710
                     /gene="msrB"
                     /locus_tag="ACL297_000363"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGR0070687.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            63768..65492
                     /locus_tag="ACL297_000364"
     CDS             63768..65492
                     /locus_tag="ACL297_000364"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070688.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            65494..67242
                     /locus_tag="ACL297_000365"
     CDS             65494..67242
                     /locus_tag="ACL297_000365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070689.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDTFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
BASE COUNT        19611 a        12517 c        15223 g        20071 t
ORIGIN      
        1 tggctacagg attttactat ggtacaccat ctgaaaatga gcagttgttt ggtgctcgtc
       61 gtaaaatccc tgagtacaag tttgtcgctg aagtggtttc ttatgatgat gcgacacaaa
      121 cagcaaccat tcgtcaacgg aacgtcatta acgaagggga ccaagttgag ttttatggtc
      181 caggtttccg tcattttgaa acctatattg aagatttgca tgatgccaaa ggcaataaaa
      241 tcgaccgcgc tccaaatcca atggaactat tgactattaa agtcccacaa cttgttcaag
      301 ctggagacat ggttcgagct cttaaagagg ggcttatcaa tctttataag gaagatggaa
      361 ccagcgtcac agttcgtgct taatgtagtt gtttagtttt aaaaaattat gcaaagctcc
      421 atatacaaca cttaaacgag attaaagaat ggcgaaatcc cttgatgcgc aagagattag
      481 ctgtcctttt ttatttttta agtgataaag tcggagctta ggtattaaag cctatcaaat
      541 taaacaaaga agcgatgtct tagatatttt gaaaaaaatt aataagcaga aaactctcta
      601 ttattttgtt gtagagagtt ttttgttaat aaaattttac aaaatgacat ttatatattg
      661 cattaagtta gatatatgat ataatattgt taaaaagagg cgcaactttt taaactcaat
      721 aagaatcaaa gagcaaacca tgaatattaa tggaggaata aaaaatgtaa gttataaatg
      781 taagtaagca ttatggtcat tcaatcattc tcaaagatat aaattttgca cttaacaagg
      841 gtgaaattgt tggtctagta gggagaaatg gagttggtaa gagtacgttg atgaaaattc
      901 ttgttcaaaa taatcaaccg acttcaggta atattataaa cagtgataat gttgggtatt
      961 taatcgaaga accaaaatta tttttatcta aaacaggttt agagaattta aaatatttgt
     1021 caaatttata tggtgttgac tacaatcaag aaagatttag aagtttgatc caagagttag
     1081 atttgactca gtctattaat aaaaaagtaa agacctattc tttgggtaca aaacaaaaat
     1141 tagctttgct tctaactctc gttacggaac ctgatatatt gattttagat gaaccgacta
     1201 atggtttaga tattgaatca tcacaaatag ttttagcggt tctaaaaaaa attagcttta
     1261 aatgaaaacg tgggaatttt aatatcgagt cataaattag aagacattga agaaatttgt
     1321 gagagggttc ttttcttgga gagcgggctt ttgacatttc aaaaagtagg aaaagatagt
     1381 cataatttct tgtttgagat agctttttca tcagctacag atagagacat tttcattacc
     1441 aaacaagaat ttggggatat tgttcaggaa gagggattga gaattactat gtctgggaat
     1501 attcaaaata gtgagctttt taaatttttt aacgaaaact ctattaaagt agttgatttt
     1561 gaaactaaaa aagagacgct taaagatatt tacctaaatc gttcaaaata aaggaaggtt
     1621 ataatcatga aattaaataa acagaagaat cggatgattt acgtcttgtc taattttcta
     1681 tatgccatct cagtttccat tatttatgct ttgaatggca ttgtgttact agtcatagta
     1741 agtaaattgg gtattccagg tgatttagga ttagatttta tagtagctat tgtagtcaat
     1801 acaattttgt tagtcctgtt ttattttcta ttatcttaca ttttttattt atacaaattg
     1861 aaaagtggct tggtatttgg tattttagta gctttactac tctttatccc taatatatta
     1921 aatacgatga tgatgaatac tagtaatgat ttgtttatca aagcaattga acttcttcct
     1981 ttttattctt tacctgtatt tgtggcttca aatacgatgt ctattagtca gtatcttgtg
     2041 gtaatcacta caatcatttt attgtacttt ttcactctca agaaaagcaa gaagtattca
     2101 ttttagtttc atttagtatt attttgcata cctaaaatac aataaaaaaa tcagtcatct
     2161 tggtatgctc ctgctttcac tattcaacac gtttttgact tatactaggc tcatttccaa
     2221 aagcattata taatagtgat atgaaaccaa ctaaactaaa caagaaatat aagcaataaa
     2281 aactcgttta aaagatccta ctaaagctaa tactaaataa aaataaaaga gtaaactagg
     2341 aagtttattt caaacaacct aaaatactga ttttcggctg aagataatac tggagtgcaa
     2401 attaatgggg ttataataaa tagctgatag cttgtgttgg ttttggattt tttaagagta
     2461 gatgagtatt aaaactataa ggaggacgaa ggtggctaaa aatttaaaat taaaattagc
     2521 tcgggtagag cgtgatttaa cacaaggtca actggcagag gctgtcgggg tgacgcgcca
     2581 gactattggt ttgatagaag cggggaaata caatcccagt ctctcgctct gccagtccat
     2641 ttgcagatgt ttagggaaaa ccctagacca actattttgg gaggaagaag atgaaaaata
     2701 gattttatta ttatcaatta ctagacgaaa gagaagaaca actgttcaat aaagcgggct
     2761 ctgaaagttt ctatatctgc atcgctttgt cgctcctatc ttatatcatt tcagtattag
     2821 caccaagcct ttttaattct aatatgctgc taatcgttat catcataggg acattttact
     2881 ttttcaatcg tgcccgttat ctgggagtga cctactatag tcgttttcat tttacgattt
     2941 tgggttgttt tttcctaacc ttggctatta cggctctttt gatgttgcag aattatcaat
     3001 tcaacataga aatttatcag cacaatcctt tgaattttaa atacctgtct gcttgggtca
     3061 ttacttatat catttacctt ccgtggatct ttattggcaa tcttggtctt aagagctatg
     3121 gcgaatgggc tcagaaaaaa tttgaacaag atatggatga attggagagt ggagaatagc
     3181 ttgttactct tttctcaatc cagctaaaat gtgatataat agtactaatt tattggaata
     3241 catgaaagtt cttgaaaatt ttcatgggtt tctagctaag gaagtaggaa aagtatgtat
     3301 ccagatgata gtttgacatt gcacacggac ttgtaccaga tcaacatgat gcaggtttac
     3361 tttgaccaag ggattcacaa taagaaggcg gtctttgagg tgtatttccg ccaacagcct
     3421 tttaagaacg gctatgcggt ttttgcaggt ttggaaagaa ttgtgaacta tcttgaagac
     3481 ttgcgttttt cagatagtga tatagcctat ttggagtcgc ttggttatca tggggcgttc
     3541 ttggattacc ttcgcaattt caagttggag ttgaccgttc gttctgccca agaaggggat
     3601 ttggtttttg ctaatgaacc gattgtgcag gtggaaggac ctctagccca atgtcagttg
     3661 gtcgaaacgg ctcttttgaa catcgtcaac taccagacct tggtggcgac gaaggcagct
     3721 cgtattcgtt cggttatcga agatgaaccc ttgatggagt ttgggacacg tcgggctcaa
     3781 gaaatggatg cggccatctg gggaacacgc gcagctgtga ttggtggcgc caatggaact
     3841 agcaacgtgc gtgcgggtaa gctctttgac attcctgttt tgggaaccca tgcccatgcc
     3901 ttggtacagg tttatggcaa tgactatgaa gctttcaagg cttacgctgc gacccacaaa
     3961 aattgtgtct ttcttgtgga tacctatgac acccttcgca tcggtgtacc agctgccatt
     4021 caggtggcgc gtgagctggg tgatcagatt aactttatgg gtgtgcggat tgactctggg
     4081 gatattgcct acatttctaa gaaagtccgt cagcaactgg acgaggctgg atttacagag
     4141 gctaagattt atgcttctaa tgatttggac gaaaatacta tcctcaatct caagatgcaa
     4201 aaggccaaga ttgatgtctg gggcgtgggt accaagctga ttacagccta tgaccagcca
     4261 gctcttggtg cagtttacaa gattgttgca atcgaagatg aaactggtca gatgcgcaat
     4321 acgattaagc tgtctaataa tgctgaaaaa gtttctacgc caggtaagaa gcaggtgtgg
     4381 cgcattacca gtcgtgaaaa aggcaagtca gaaggcgact atatcactta tgatggtgtg
     4441 gatattagcg acatgacaga aatcaagatg ttccatccga cctatacata catcaagaag
     4501 acggttcgta attttgatgc cgttcctctc ttggtggata tcttcaaaga aggaatatta
     4561 gtttacaact tgcctagttt gactgacatt caggattatg cccgtaaaga atttgacaag
     4621 ttgtgggatg agtataagcg tgtgctcaat ccgcagcact atccagtgga tttggcgcgt
     4681 gatgtatggc aagataagat ggacttgatt gataagatgc gcaaggaagc ccttggtgaa
     4741 ggagaagaag aatgagtttg caagaaacga ttatccaaga gctgggtgtc aaaccagtga
     4801 ttgatgccca ggaagaaatc cgtcgttcta ttgatttctt aaaaagatat ctgaaaaaac
     4861 atcccttcct aaaaaccttt gtactaggga tttctggggg acaagactca accttggcag
     4921 ggcgtttggc gcaattagct atggaagaac tgcgagctga aacgggagac gatagctaca
     4981 aatttatcgc tgtccgcctg ccatacggag tgcaagctga tgaagcagat gctcaaaaag
     5041 ctctagcctt catccagcca gatgtcagct tggttgtgaa tatcaaggaa tcagctgatg
     5101 ccatgacagc tgcagttgaa gcgacaggta gtcctgtttc agacttcaac aaggggaata
     5161 tcaaggcacg ttgccgtatg attgctcagt atgcccttgc tggttcccat agcggagcgg
     5221 tcattggaac agaccacgcc gcggaaaata tcacaggttt ctttaccaag tttggtgacg
     5281 gcggtgcgga tattctccct ctttaccgcc tcaataaacg ccaaggaaaa cagctcttgc
     5341 agaaacttgg cgcagagcca gccctttatg aaaaaatccc aacggcagac ctagaagaag
     5401 ataaaccagg cctagctgac gaagtcgcac ttggagtcac ctacgcagag attgacgact
     5461 acctagaagg caaaacaatc agcccagaag ctcaagcgac cattgaaaac tggtggcaca
     5521 aaggccaaca caaacgccac ttacccatca ctgtatttga tgacttttgg gagtaaaaag
     5581 gtccggggga cctttttagc ttcttgccct gaaattaaaa agcaagaaaa acctccactg
     5641 gaggttttca gcctctcatc ttaaaataag aaagtgagag aaggtctggg ggatcttgaa
     5701 ccccgagttt agaaataaga aaatgaggca gattcggtaa ctcgaagagt tcgatttcat
     5761 cgtcttaccc ctgcaacgat gactaggttt gaaaaagctt gctagatcgc atttcaaacc
     5821 aggcagcgat tgcgtcaaga aattagaaga caaactcgtt ttctagctgt tactgagttg
     5881 agccttttta ctacgagcat agaaataagg aagtgaggta gcatcatgaa atctattggt
     5941 acgcaaatat tacagacaga acgtttgatt ttaagaagat ttgtggagag tgatgcagaa
     6001 gctatgtttc aaaattgggc ttcatccgct gagaatctga cctatgttac ctgggatccc
     6061 catcctgata tcgaaatcac tcgaaactcg attcgcaatt gggttgcttc ctatactaat
     6121 ctcaactatt ataaatgggc catttgtcta aaagaaaacc cagagcaagt gataggagat
     6181 atcagcattg ttaagataga cgaggctgat ttaagctgtg aaatcggcta tgtgctaggc
     6241 aaggcttact ggggacatgg tatgatgaca gaggccttga aagctatctt ggacttttgt
     6301 tttactcaag caggttttca aaaggtcaga gcacgttatg ccagtctcaa cccagcttca
     6361 ggtcgtgtca tggaaaaggc tggaatgtcc tatctacaaa ccattgttaa tggtgtagag
     6421 agaaaaggct atcttgcgga tcttatttat tatggtataa gtagggaaga atgttgaatt
     6481 ctattttctg tttctatcga agtcaactat ttattgtaaa tataataatt agcattccaa
     6541 gtttatttga aactttaaaa tagcatattg attagtacaa gacagatgtt ctagttcctt
     6601 ctttaatctg gtttagtatt agttaaaaaa tcgctttaag cttgtaacta agagggacct
     6661 aatcgactag attctccagc cgaacaggtg gtaatgtact ttttatagtg taatcctagc
     6721 tgttgttaaa tttaaaatag aatcctctat cgagttaggg aattaaattc aaccaatttt
     6781 attcatgttt tttctatcaa attatctaat attaaaatag tctcattctg atgagaaaac
     6841 tattctattt atactgcata gttgtataag gaatgatgaa agatgagtga aaattatcaa
     6901 gttggaatgt ttgtatctaa atatattagc atgtatttag ataagatgtc tgcaatcctt
     6961 tatatatgag attgattacg atatagttaa agtgttagtt caataatcaa tatatcattc
     7021 taagtctatt ttatatataa agtatattgt ttaacgtttg atagaaaaga tgctatacta
     7081 gaagtaggat gtaatggcaa aatctaatac taataagcgc tatgcaatac gtaaactcgg
     7141 aaagattttt ggttctgcgg taatcgggct aactttattt ttaggaggaa tctgaataca
     7201 agttttgttc atgctgctga tgggattcaa tatgtcagag atgatactag agataaagaa
     7261 gagggaatag agtatgatga cgctgacaat ggggatatta ttgtaaaagt agcgactaaa
     7321 cctaaggtag taaccaagaa aatttcaagt acgcgaattc gttatgaaaa agatgaaaca
     7381 aaagaccgta gtgaaaatcc tgttacaatt gatggagagg atggctatgt aactacgaca
     7441 aggacctacg atgttaatcc agagactggt catgttaccg aacaggttac tgttgataga
     7501 aaagaagcca cggatacagt tatcaaagtt ccagctaaaa gcaaggttga agaagttttt
     7561 gttccatttg ctactaaata tgaagcagac aatgaccttt ctgcaggaca ggagcaagag
     7621 attactctag gaaagaatgg gaaaacagtt acaacgataa cttatgatgt agatggaaag
     7681 agtggacaag taactgagag tactttaagt caaaaagaag actctcaaac aagagttgtt
     7741 aaaaaaggaa ccaagcccca agttcttgtc caagaaattt caatcgaaac agaatatctc
     7801 gatggcccaa ctcttgataa aagtcaagaa gtagaagaag taggagaaat tggtaaatta
     7861 ctcttactac aatctatact gtagatgaac gtgatggaac aattgaagaa actacttctc
     7921 gtcaaattac taaagagatg gtaaaaagac gtataaggag agggacgaga gaacctgaaa
     7981 aagttgttgt tcctgagcaa tcatctattc cttcgtatcc tgtatctgtt acatctaacc
     8041 aaggaacaga tgtagcagta gaaccagcta aagcagttgc tccaacaaca ggctggaaac
     8101 aagaaaatgg tatgtggtat ttttataata ctgatggttc catggcaaca ggttgggtac
     8161 aagttaatgg ttcatggtac tacctcaaca gcaacggttc tatgaaagtc aatcaatggt
     8221 tccaagttgg tggtaaatgg tattatgtaa atacatcggg tgagttagcg gtcaatacaa
     8281 gtatagatgg ctatagagtc aatgataatg gtgaatgggt gcgttaacag attcctccct
     8341 gcgacaaaat tgataatagt atgtctgaat atataattat atataaaaag ctatagataa
     8401 ggtcatctta tagcgactat aaaaaagtga acaagcctag tcttctgtac attagaattc
     8461 agatttgttc actttttgtt taatactcaa tgaaaatcaa agagcaaact agaaaactag
     8521 tcgcaggttg ctcaaagtac tgctttgagg ttgtagatag aactgacgaa gtcagtcaca
     8581 tatataatcc aaggtgacgt tgacgcggtt tgaagagatt tttgaagagt ataagtcttt
     8641 gattttgaga ctaaggagag tcagggtcaa agtagtgaat gattctgaaa taactcataa
     8701 tagtctcttg agaggacaga taagagtaac aaatatggtg ctactcatgt gtcttgcatt
     8761 ttgatttaca aacagcgaag aaaatatagg aagctgaaat aaaatataga tgaatcaatt
     8821 tggagagtca acgcaatcta tggcaatatt ttcgaagcta gggggaagca ttttaatttc
     8881 aatgtactag gcgtcttcta cagctagcta gagtttcaat tgtagatgtt ctttgaaata
     8941 atatcttagc gatttattgg tattagaaga attagagaaa ttctttctca ataaataaac
     9001 atggcgtcac caaaactgaa gaagcggtag tgttcttgga tggcatggtg gtaggcatct
     9061 aagactaatt cacggcctgc aaaggcagaa accaacatga ccagagttga ttttggcaga
     9121 tggaagttgg ttgagaaggc atccacgacc ttccactcat acccaggttt gataaagata
     9181 ttggtccaac cagaatctgc ttggatttgc ccatcaaact tggaaccaat agtttccaag
     9241 gtgcggatag aagtggttcc gacagcgatg acgcgaccac cattttcttt gacagagcga
     9301 agggtggcag cagcttcctc agaaagttga tagaactctg agtgcatttc gtgttcgtcc
     9361 agattatcca cagaaacagg tctaaaggtt ccgagtccga catggagagt cagatagact
     9421 agatgaacac ccttagcttg gatttctgcc agcagttctt tggtgaagtg aagaccagca
     9481 gtcggtgctg cagcagagcc gctttccttg gcgtagacgg tttgataacg ttcacggtca
     9541 tctaattttt cgtggatata aggtggcaga ggcatttctc ccagactttc caagacttct
     9601 aggaaaattc cttggtattc aaagcggaca atgcgtcccc cgtgggtcaa ttcttctgta
     9661 acgacagcgc tgaggcggcc atcaccaaag ctgatacgag taccgacctt gaggcgtttg
     9721 gcaggtttag ccagaacttc ccactcgtct ccactagtgt tcttaaggag gagaagttcc
     9781 acatgacctc ctgtctccac tttttgacca tagaggcggg cagggagaac tcgggtgtcg
     9841 ttcatgacaa gggcatcacc aggttccagc atatcaataa tagagtagaa gtgtttatct
     9901 tgcatttctc ctgtctcacg gttgacgatg aggagtttgg aggcatcacg tttttcaagg
     9961 ggcgtttggg caatcaattc ctctggcaag tggaaatcaa aatcagctgt attcatatat
    10021 ttgttcattc tttctaagtt ctaatcctat ccattataac acgtttcaag tttggacgct
    10081 ctttttttag aaattaaatc gttttcactt gacaaaaatt ggtctatacc atataataaa
    10141 tatagacaga aatggaggat gaaaagatga aagttattaa agttgaaaac caagttcaag
    10201 gtggaaaagt tgcttttgag attttgaagg aaaaattggc taatggagct caaactctag
    10261 gacttgcgac aggaagtagc ccacttgaat tttacaagga aattgttgag agtgaccttg
    10321 atttttcaaa tctaaccagt gtcaatcttg atgagtatgt gggccttgat ggggacaatc
    10381 cacaatctta tcgttacttc atgcaagaaa acttgttcaa ccaaaaacca tttaaagaaa
    10441 gtttcttgcc tcgtggtgtt aaggacaatg ctgaagctga agttgaacgc tacaaccaaa
    10501 ttttggctga ccatccagtt gacctccaaa tcttgggaat cggtcgcaat gggcatatcg
    10561 gctttaatga gcctggtact ccatttgaca gtcaaacgca tctagtagaa cttgaccagt
    10621 ctactatcga agccaatgca cgcttctttg ccaagattga agacgtccca acccaagcta
    10681 tttcaatggg gattaaaaac attttggatg ccaagtctat tattcttttt gcttacggtg
    10741 agtcgaaagc agaagccatt gccggaacag tatctggccc agtgactgag aacctacctg
    10801 caagtagcct tcaaaatcac cctgatgtaa ccatcatcgc agatgctgaa gcgcttagct
    10861 tactcgaaaa ataaaaaagc aatgttctat tgaacgcttt caactcttgt ataaatggaa
    10921 gaaaaaatca aaattaaacc gcatttttgc ttgacaatta ttccttttac gtgtagaatg
    10981 aaatagatat tgaacttgaa gggagtgaaa aaaatgtcta aaacagtagt acgtaagaat
    11041 gaatctcttg acgacgcact tcgccgtttc aaacgtgcgg ttactaaagc tggtactctt
    11101 caagaaacac gcaaacgtga attctatgaa aaaccttctg taaaacgtaa acgtaaatca
    11161 gaagtagctc gtaaacgtaa aaaattctaa ttgaaatgaa aggctagaga aatctagtcc
    11221 tttttctttt aaataaatac tccaaagcct gcaaaaatct gaaacttcct cctacaattt
    11281 gatataatag agagaagaac tcatttgaag gaggaaatga tgtcggtttt agtaaaagaa
    11341 gtgattgaaa agcttagact agacattgtc tatggtgaac cagagttgct tgaaaaggaa
    11401 atcaatacag cggatattac gcgacctggt cttgaaatga caggctattt tgactactat
    11461 acaccagagc ggattcaact tttggggatg aaggagtggt cttatctgat cagcatgcct
    11521 tccaacagcc gttatgaagt tttgaaaaaa atgtttctac ctgagacacc agcagtcatt
    11581 gttgctcgtg gtttggtggt tccagaggag atgttaaagg ctgctagaga atgtaagatt
    11641 gctattttaa ccagccgtgc agctaccagt cgtttatctg gagagttatc tagctatctg
    11701 gattctcgtt tggcagaacg taccagtgtg cacggtgtct tgatggatat ttatgggatg
    11761 ggcgtcttga ttcagggaga tagtggaatt ggtaagagcg agacaggtct tgagcttgtc
    11821 aaacgtggtc accgtttggt agccgatgac cgtgtcgata tctttgccaa ggatgagatt
    11881 actctctggg gtgaaccagc tgaaattttg aaacacttga ttgaaattcg tggggttggt
    11941 attatcgatg ttatgagtct ctacggtgcg agtgctgtca aggattcttc acaggttcag
    12001 cttgctgtct atttggaaaa ttacgataca cataagacct ttgatcgtct tggaaacaat
    12061 gcagaggaac ttgaagtttc tggcgtagcc attcctcgta ttcgtattcc agttaaaaca
    12121 ggtcgtaata tctctgttgt gattgaggca gctgccatga attatcgtgc caaggaaatg
    12181 ggctttgatg cgacccgttt gttcgacgaa cgactgacaa gtctcattgc tcgaaatgag
    12241 gtgcaaaatg cttgatccaa ttgctattca actaggaccc ctagccattc gttggtatgc
    12301 cttatgtatt gtgacaggct tgattcttgc ggtttatttg accatgaaag aagcacctag
    12361 aaagaagatc ataccagacg atattttaga ttttatctta gtagcctttc ccttggctat
    12421 tttaggagct cgtctctact atgttatttt ccgatttgat tactatagtc agaatttagg
    12481 agagattttt gccatttgga atggtggttt ggccatttac ggtggtttga taactggggc
    12541 tcttgtgctc tatatctttg ctgaccgtaa actcatcaat acttgggatt ttctagatat
    12601 tgcggcgcct agcgttatga ttgctcaaag tttggggcgt tggggtaatt tctttaacca
    12661 agaagcttat ggtgcaacag tggataatct ggattatcta cctggcttta tccgtgacca
    12721 gatgtatatt gaggggagct accgtcaacc gactttcctt tatgagtctc tatggaatct
    12781 gcttggcttt gctttgattc tgatttttag acggaaatgg aagagtctca gacgaggtca
    12841 tatcacggcc ttttacttga tttggtatgg tttcggtcgt atggtcatcg aaggtatgcg
    12901 aacagatagt ctcatgttct tcagccttcg agtgtcccaa tggctgtcag ttgtccttat
    12961 cggtctcggt ataatgatcg ttatttatca aaatcgaaag aaggcccctt actatattac
    13021 agaggaggaa aaataaatgt tagaagttgc atatattctt gttgccctag ctttgattgt
    13081 ctttttggtc tatctgatca ttactgtaca aaagcttggt cgtgtcatcg atgaaacaga
    13141 aaagacgatt aaaaccttga cttcagatgt ggatgtgacc ttgcatcaca ccaatgagtt
    13201 gttggctaag gtcaatgtct tggcagatga tatcaatgtc aaggtggcta cgattgatcc
    13261 actcttcagt gctgttgcag atttatctct atctgtttca gacctcaatg accatgcgcg
    13321 tgtcttgagc aagaaagctt catcagctgg ttcaaaaaca ctcaagactg gtgcaagtct
    13381 gtcagctctt cgtcttgcaa gtaaattttt caaaaaataa aaaaggagaa tccttatggg
    13441 taaattatcc tcaatccttt taggaacggt ttcaggtgca gctcttgcct tgtttttaac
    13501 aagtgataag ggcaaacaag tttgcagtca ggctcaagat tttctagatg atttgagaga
    13561 agatccggag tatgccaagg agcaagtctg tgaaaaactg acagaagtta aggagcaggc
    13621 tacagatttt gttctgaaaa caaaagaaca ggttgagtca ggtgaaatca ctgtggacag
    13681 tatacttgct caagctaaat cctatgcttt tcaagcgaca gaagcatcaa aaaatcaatt
    13741 aaataatctc aaggaacaat ggcaagaaaa agccgaagct cttgatgact cagaagagat
    13801 tgtgattgat ataacagaag aataaaccat caccatctcc ggacggacta tgtatctggg
    13861 gatggtgatt tttatctgga atctagtctt tgtggtataa taattactat gcagaaaaaa
    13921 ccaacgtcag cctatgtgca catcccattt tgtacccaga tttgttatta ttgtgatttt
    13981 tcaaaggtct tcatcaaaaa tcagccagtc gacagctatt tagagcatct gctggaagag
    14041 tttcgttctt atgatattga aaagttgtca accctttata tcggtggtgg aacaccgaca
    14101 gccctgtcgg ctccgcaact ggaggtgtta ctgaatggct tgactaaaaa cttggacttg
    14161 tctgtcttgg aagagttgac cattgaagcc aatccaggcg atttggatgc ggataagata
    14221 gctgttttga aaaattcggc tgtcaatcgt gtttcgctag gtgtccagac ctttgatgat
    14281 aagatgttga aaaagattgg gcgcagtcat ttggagaagg atatttatga aaatatcgat
    14341 cgtctgaaac tggctggttt tgacaacatc tccattgatt tgatttatgc tctgcctggt
    14401 cagaccatgg agcaagtaaa ggaaaatgtg gctaaagcca ttggactgga tattccccac
    14461 atgagtttgt atagtttgat tttagaaaac catacggtct ttatgaaccg gatgcgacgt
    14521 gggaaattgc ctctgcctaa ggaggaacta gaagcggaga tgtttgagta catcattgca
    14581 gagctggagc gagcgggttt tgagcattat gagatttcta atttctccaa acccggtttt
    14641 gaaagtcgtc acaatctcat gtactgggac aatgctgaat actatggtat tggtgcaggg
    14701 gcatctggtt atgtcaatgg agtacgctat aaaaatcatg gtcccattcg tcattatctc
    14761 agtgcggttg aggaaggcaa tgcttgtatt acagaagatc acctgagtca aaaggagcaa
    14821 atggaagaag aaatgttctt gggactccgc aagaaatccg gggtttccat ggcgcgattt
    14881 gaggaaaaat ttggacagtc ttttgctgga ctttatggag aaattgtcag agatttggtt
    14941 caacaaggcc tcatgcagat tgagggtgac cacgttcgca tgacaaagag aggtctcttt
    15001 ttgggagata ctgtagcaga acgatttatt ttggagtagg atcatgggct taacttatca
    15061 aatgaaaatg aaaattcctt ttgatatggc tgatatgaac ggtcatatca aacttccaga
    15121 tgtgattttg ctgtcactgc aagtttcagg gatgcagtcg attgaactgg gagttagtga
    15181 taaggccatt ttggaagaat ataatctggt ctggattatc gtagaatatg atattgaggt
    15241 ggttcgtttg cctcgttttg cggaagaaat taccatcgaa acggaagcct tgagctacaa
    15301 tcgacttttt tgctaccgtc gctttactat ttataatgaa gcggagcagg agctcatcca
    15361 catgatggcg acctttgttc tcatggaccg cgacagtcga aaagtccatg ttgttgaacc
    15421 tgagattgtg gctccttacc agtctgattt tgataaaaaa cttatccgtg gaccgaagta
    15481 tgagtccttg gaagaaccga tcagcaagga ttaccatgtt cgtttttacg acttggatat
    15541 gaatggtcat gtcaataaca gtaaatactt ggactggatt tttgaggtca tgggagcgga
    15601 ttttttgacc cactatattc ccaagaaaat caacctcaag tatgtcaagg aagttcgacc
    15661 aggtggggtg attacatcgg ctgttgaacg gactggactg gaaagcaagc atgaaattac
    15721 aagtgatggg gttatcaatg cccaagcaat cattacttgg caagaaataa aaaaggctta
    15781 gggagaaata gatgaaatat aaaggctatt taattgattt agacggaacc atttataagg
    15841 ggaaagaccg aatccctgct ggagagactt ttgttcatga attgcaaaag cgggacattc
    15901 cctatctctt tgtaaccaac aatacaaccc gcactccaga gagtgttaag gagatgttgg
    15961 ctcagaattt taatatcgat acgcccctat cgactgtcta cacagcgact ttggcaacca
    16021 tcgactatat gaatgatttg gggcttgaaa agaccgtcta tgtcgtcgga gaagcaggac
    16081 tcaaggaagc catcaaggcg gctggttatg tggaagacaa ggaaaaacct gcctacgtgg
    16141 tagttggtct ggattggcaa gtcgactatg aaaaatttgc cacagcaact cttgctattc
    16201 aaaagggtgc ccattttatc ggaaccaatc ctgacctcaa catcccgacg gaacgcggtc
    16261 ttttgccagg tgctggctca ctgattacgc tgcttgaagt agcgacacga gtgaagccgg
    16321 tttatatcgg aaaaccaaat gccatcatta tggacaaggc ggttgagcac ttaggtttgg
    16381 aacgtgaaga gttaatcatg gttggggaca attatttgac tgatatccgg gctgggattg
    16441 acaatggcat tccaacgctc ttggtgacga caggttttac caaggcagaa gaagtagctg
    16501 gcctgccaat cgcaccgaca catgtggttt ctagccttgc ggagtgggat tttgatgaaa
    16561 actaaattga ccttttgggg ctctatgctc tttctcctct ccctctcaat ccttctgacc
    16621 atttatctgg cttggatttt ctatcctatg gagattcagt ggctaaactt aacgaatcga
    16681 gtctatctaa aaccagaaac cattcaatac aattttcata tcttgatgaa ttatctgacc
    16741 aatcctttta gtcaggtctt acagatgcct gattttcgtt cgtcagcagc tggtctgcac
    16801 catttcgcag tggtcaagaa tctctttcat ttggttcagc tagtagctct agtgacactg
    16861 ccaagtttct atgtctttgt caataggatt gtgaaaaagg actttttgtc tctttatcga
    16921 aaaagtctcc tggctctagt agtcttacct gtgatgattg gacttggggg agttttgatt
    16981 ggttttgacc aattctttac tcttttccat caaattctct ttgtgggaga tgatacctgg
    17041 ctttttgatc cagccaagga tcctgttatt atgattttgc cagagacctt ctttcttcat
    17101 gccttcctcc tcttttttgc cctctatgaa agcttctttg gctatctgtg tctgaaaagt
    17161 cgtaggaagt gaaatgaaag atgtttttaa tttgcatata taaaaatata tatcgtttaa
    17221 aaccatcgtt aagagtgaat taaccaaatc caattgtgtc aatcgttcgc aaacagccta
    17281 tttttcttga aaaaataggc tttttttcta aaaatcccta gtcaagcgcc ttattttttt
    17341 gtataataga agtagcaaag tatagataag aagagaaaag catgattaca ctatttctat
    17401 caccgagctg tacatcatgt cgtaaggcta aggcctggtt agaaaaacat aaggttccct
    17461 ttgtggaaca caatattatg accagtcctt taacaagaaa agaattgcaa cacatccttt
    17521 ccttgaccga aaatggtact gatgacatca tttcaactcg ttcaaaaatt tttcaaaaat
    17581 tgaatattga tgtagagagt atttcggtat cggaattgct tcatttgatt gagcagtatc
    17641 ctagtctttt gcgtcgtcca attattattg atgccaagcg tatgcaaatc ggttttaatg
    17701 aagatgagat tcgtgctttt ctccctcgta gttatcgtaa gcaagaacta aaagaagcaa
    17761 gaatgagagc tggtattagt taatgaacaa acagtatagt tacccactag atttgtcgtg
    17821 gagcactgaa gaacttgctt cagtgctttc tttttttaat gatgttgaag ctgcctatga
    17881 aggcaaagta gaggctaaaa agttactgga ctcctataag ggattcaaag cggtcgtgcc
    17941 aagcaagagc gaagagaaac gtttgggacg agaatttgaa acggttagtg gctattcact
    18001 ttaccgagca gttcaggctg ccaaagaaaa aggggaagga aagatttctc ttggaaagta
    18061 aatttgaatt tgctaaagag cttgttaaga aagcaggtca gtacattctt gaccatatgc
    18121 aggaagactt gcgtgttgaa accaagtcct ctccaacaga tttggtgacc agactggaca
    18181 aagaagttca ggaactcttg gttggtgaga ttttgtcccg ttatcctgag gataagattt
    18241 gtgctgaaga aggttgtctg cgagcctcgg ttcaagaggg caaggtttgg gtcattgatc
    18301 ccattgatgg taccaataat tttgtagccc agcaggaaga ttttgctgtt atgatggctt
    18361 attttgaaaa tggtcaggga cagtttggtc tgatttatga tgtggtcaaa ggggattgtt
    18421 accacggtgg tggggaattt ccagtttgtc taaatgatag gcccctagct ccttttaaaa
    18481 ctaaaccact tggagatttt ctcattgcag ggaatagtgg tatgctggaa accaatgaat
    18541 gggggctggc tgatttgagc cgagcggttc ttggtgttcg tgtctatgga agtgcggcca
    18601 ttagttttgc caagattttg tcagggcgtt tgttgaccta tctcacttat ctgcaaccat
    18661 gggattatgc cgcagctagt attttagggg aaagtctggg ctatcgggtt gtgacccttt
    18721 ttggtgaagc tcctgatttt caaaccagac agccggtcat gatggtgcct cttgagatgc
    18781 aggaggaaat tcagtcctat atttacgaaa ggaaaagaac ttaaatgcaa tttccagaag
    18841 gatttgttga aaaatataaa gagatactag gagatgaggc aagagatttt cttgcctctt
    18901 ttgaggagga agcggtttcg gcctttcggg tcaatccctt aaaagaagag caactttcct
    18961 tttctgatgc cattactcaa accccttggg gccactatgg gaaggtttca gggaaatcgc
    19021 ctgagcatgc tacaggttta gtttattcgc aagaacctgc tgctcaaatg gtggctcaag
    19081 tagctcaacc cagtcctggt atgaaggtct tggacttggc tgctgcaccg gggggcaaat
    19141 caactcaact ggcagcctat ctagcagggg aggggctact tgtttccaat gaaatttcaa
    19201 gcaaacgggc taagattttg gttgaaaaca tggagcgttt tggagcgaca aatgtcgtgg
    19261 tgaccaatga atctgccgac cgcttggtca aggtctttaa aggctatttt gacttaatcg
    19321 ttcttgatgc cccttgctct ggtgaaggaa tgtttcgtaa gcaacctgat gctatggact
    19381 attggagctt agattatccg agtcaatgtg ctagcttgca aagagaaatt ctggaggatg
    19441 cagtgaccat gttagctgaa ggtggtcgtc tggtttattc gacctgtacc tgggcacccg
    19501 aggaaaacga agagattgtc aattggttgc tggaagagta tgattttgac ttgttgccag
    19561 tagagcatat caatggaatg gtagctggta ttgacctgcc agaaacggct cggatgtacc
    19621 ctcatcagtt taagggagag ggtcagtttg ttgcccatct acagtttaag ggtaacaatc
    19681 cagctcctaa atttaaggca agtaagagta acctcagccg tgaacaagtt gccttgtggc
    19741 aggaatttgc ccaaaaccat ttgaaggtca atctaccggg tatcttgcag acttttggag
    19801 accagcttta tctcttgcca gaacttttgc cagatttagg gaagctcaag attgctcgca
    19861 atggactgca tctgggcact tttaagaaga aacgctttga gcccagtttc gctcttggtt
    19921 tagccttgaa accgagtcag gtcgaacagt cggttgaaat tggccaagaa gcgtttgtca
    19981 agtatgcggc tggagaaatc gttcagctgg cagaaagtct gccaaatggt tggtaccaag
    20041 ttttggttaa aggcaatggc ttgggctttg caaaggttac tggaaatgtt ttgaaaaatt
    20101 attttccaaa aggcctcaga ttcaaatgaa aatgctagtc aaatatctta ttctatgtta
    20161 tagtgaaagt atggattttt ttagtaggat gattcttgat atcttgattg ccattacatg
    20221 tagttttact actcaatgaa aatcaaagag caaactagga agctagccat agatagctca
    20281 aaacactggt ttgaagagat tttcaaagag tacaagaagt ttagttatta gcgttcttac
    20341 cgcttgtaaa ctagatttct cataaaatag aatcttttcc ttttagttgt aaactagtct
    20401 gggagagtag agaggtttga gatacctttc tagcttttgg attatcatct aaggagagta
    20461 atttcccttg cattaaaaag gggaaaaaga gacacgaaat gactataatg ggtgacaatg
    20521 ggggaaggga tagacaagag attttatcca catatgaaaa aaggaggtta ggaaagagta
    20581 atatagccta tattatataa ataatcaatt gcgcagaaat ttggtaagaa ttcatgcgtc
    20641 aactcataaa gaactactta aaaaattcac agtattcata attattttcg aggagaaaaa
    20701 cagtgaaaaa aagaaaaaag cttgctctgt ctcttatcgc tttttggctg acggcttgtt
    20761 tagtaggctg tgctagctgg attgatcgtg gagaatccat aacggctgtt ggctcaactg
    20821 ccttgcaacc cttggttgaa gtagcggcag atgaatttgg cacgatccat gttggaaaaa
    20881 cggtcaatgt ccaaggggga ggttctggta caggcttgtc ccaggttcag tctggggcag
    20941 ttgatatagg aaactcagat gtatttgctg aggaaaaaga cggaattgat gcttctgctc
    21001 ttgttgacca caaggtcgcg gtagctggct tggctctgat tgtcaataag gaggttgatg
    21061 ttgataacct aacgacagag caacttcgtc aaatcttcat aggtgaggta accaattgga
    21121 aagaggttgg tggtaaggac ttacccatct ctgttatcaa tcgggcagcc ggctctggtt
    21181 ctcgtgctac ctttgatact gtcattatgg aaggtcagtc tgccatgcaa agtcaggagc
    21241 aggattcaaa tggagcggta aaatcaatcg tatcaaaaag tccaggagct atctcttatt
    21301 tatctcttac ctatatagat gattcggtca aaagcatgaa gttgaatggc tatgacttaa
    21361 gtccagaaaa tataagtagc aataattggc ccttgtggtc ttatgagcat atgtatacat
    21421 tggggcagcc caatgagttg gctgcagaat ttctcaattt tgttctctcg gatgagaccc
    21481 aagaagggat tgtcaaagga ttgaagtata ttccgattaa ggaaatgaag gttgaaaaag
    21541 atgctgccgg aactgtgaca gtgttggaag ggagacaata atgaatcaag aagaattagc
    21601 taagaaaatg ttgcttccat caaagaattc tcgtctggag aaattaggaa aaggtttgac
    21661 ctttgcctgt ctttctttga tagtcatcct tgtggccatg attttggttt tcgtagcgca
    21721 aaaaggcttg tcgaccttct ttgtcaatgg tgtgaatatc tttgactttc ttttgggagg
    21781 aacttggaat ccttctagta aagaatttgg tgcccttcct atgattttgg gttcctttat
    21841 cgttaccatt ctctcagccc ttatcgcaac accctttgct attggtgcag cagtttttat
    21901 gaccgaagta tcaccaaaag gggcgaagat tttgcaacca gctattgaac tcctggttgg
    21961 gattccttca gtagtgtacg gatttattgg cttgcaagtc gtcgttccct ttgttcgcag
    22021 tgtctttggt gggactggtt ttgggatttt gtcagggatt tccgtcctct ttgtcatgat
    22081 tttgccgacc gtaaccttta tgacaacgga tagcttgcgt gcggttcctc gttattatcg
    22141 tgaggccagt ttcgctatgg gagccactcg ctggcagact atctggcgtg tgatcttgaa
    22201 ggcggcccgt tctggtattt tcactgcagt ggtctttggg atggcgcgtg cctttggtga
    22261 ggctttagct atccagatgg ttgtcggaaa ctcagctgtt atcccaactt ccttgaccac
    22321 accagctgca actttaactt ctatattaac tatgggaatt gggaacactg tcatgggaac
    22381 tgtaaataat aatgttctct ggtcactggc cttggtactg ctcttgatga gtttagtttt
    22441 taacagtgtg attaaattga ttacgaaaga aagaggaaag aaaaattatg cgcgctaaga
    22501 aattagataa acttgcaaca gctgtcctct atacgattgc tagcatcatt gtgacaatct
    22561 tggcttcctt aattctctat atcttggttc ggggcttgcc ccatatctct tggtctttct
    22621 tgactggaag gtcttctgct tttcaagcag gtggtgggat tggcattcag ctttacaatt
    22681 cctttttcct attggtcatt accttgatta tttctgtacc tctttctatg ggagctggga
    22741 tttacttggc tgaatatgct aaaaaaggtc ctgttaccaa ctttgtgcgg acttgtattg
    22801 aaatcttgtc ctctttacca tcagtggtgg tgggtctctt tggttacttg atctttgtag
    22861 tccagtttga gtatgggttt tcaatcattt caggtgcctt ggccttgaca gtctttaact
    22921 tgcctcagat gacgcgtaat gtagaggata gtttgaaaca cgttcaccat acccaacgtg
    22981 aggctggtct ggctcttggg atttctcgct gggagacagt ggttcatgtt gttattccag
    23041 aagcgcttcc aggtattgta acgggtgtcg tcttggcatc tggtcgtatc tttggcgaag
    23101 ctgcagctct gatctataca gcagggcaat cggcgccagc tcttgactgg tctaactgga
    23161 atatcctcag tgtgactagc cccatctcta tcttccgtca agcagaaacc ttggctgtcc
    23221 atatctggaa agtcaatagt gaaggcacta ttccagatgg aaccattgta tcagcaggtt
    23281 ctgccgctgt gctcctgatc tttatcctga tttttaactt tggagctcgt aagttcggaa
    23341 gctatctaca caagaaatta accgctgcct aaaggagaag ccatgtcaac atataactgg
    23401 gatgagaagc atatccttac ctttcctgaa gaaaaagtag ccctttctac taaggatgtc
    23461 catgtttact atggtaaaaa tgaatccatt aaggggattg atatgcaatt tgaaagaaat
    23521 aaaattacag ctttgattgg tccgtcggga tcggggaaat ctacctactt acgcagtctc
    23581 aatcgcatga atgataccat tgatattgct aaagtaactg ggcagattct ctatcgtgga
    23641 attgatgtca accgtccaga aatcaacgtt tatgaaatgc gtaaacacat tggaatggtt
    23701 tttcaacgcc ccaatccatt tgctaagtca atttaccgta atattacctt tgcgcatgaa
    23761 cgtgctggag ttaaggataa gcaagtccta gatgaaatcg tagaaacctc ccttcgtcag
    23821 gctgccctct gggatcaggt taaagacgat ctccacaagt cagccttgac cttatcaggt
    23881 ggtcagcaac aacgtctctg tatcgctcgt gccatctctg ttaagccaga tatcctctta
    23941 atggatgagc cagcctcagc cttggatccg attgcgacca tgcaactaga agagaccatg
    24001 tttgagctca agaaaaactt taccatcatc attgtaacgc ataatatgca gcaggctgct
    24061 cgtgcaagtg actatacagg cttcttttac ttgggtgatt tgattgagta tgacaagact
    24121 gcaactattt tccaaaatgc caagctacag tccaccaatg actatgtatc tggtcacttt
    24181 ggttagaaag gaaaccgtat gacagatgcg attttacagg tatcagacct gtccgtttat
    24241 tataataaaa agaaggcttt gaatagtgtt tccctatctt tccaacctaa ggaaattaca
    24301 gccttgattg gtccatctgg atcagggaag tcaaccctcc tcaagtctct caaccgcatg
    24361 ggagatctca atccagaggt gaccacaact ggatccgtgg tgtacaatgg tcacaacatc
    24421 tacagtccgc gtacagatac ggttgaatta cgtaaggaaa tcggaatggt tttccaacaa
    24481 cctaatcctt tccctatgac tatctatgag aatgttgtct acgggcttcg tatcaatgga
    24541 attaaggata agcaggttct ggatgaagcc gtagaaaaag ccttgcaagg tgcctctatc
    24601 tgggatgagg tcaaggatcg tctatatgat tcagctattg gattgtcagg tggtcaacag
    24661 cagcgtgtct gcgtggcccg tgtcttggca actagtccta aaatcatcct cttggatgag
    24721 ccaacttcgg ctttggatcc gatttcagct ggtaaaattg aggaaacctt gtatggtcta
    24781 aaagacaagt acaccatgct tctggtaacc cgttccatgc agcaagcttc acgtatctct
    24841 gataagacag gatttttcct agatggagat ttgattgaat ttaatgatac caagcagatg
    24901 ttccttgatc cccaacacaa ggaaacggaa gactatatta caggaaaatt tggataagga
    24961 gatgaaagat gttacgatct caatttgaag aagatttaga gaaattacat aaccagttct
    25021 acgctatggg acaagaagtg ctctcacaaa tcaatcgtac ggtacgtgct tttgtcacgc
    25081 atgaccgtga cctggcaaaa gaggtcatcg aagatgatgc agaagtaaat gaatacgaag
    25141 tgaaactgga aaagaaatca tttgaaatga tcgcactcca acaaccagtc tctcaagatt
    25201 tgcgtacagt cttgactgtc cttaaggctg tatcagatgt ggagcgtatg ggggatcacg
    25261 ctgtagccat tgcccaggca accatccgta tgaaggggga agaacgtatt ccagctgtag
    25321 aggaagaaat taaaaaaatg ggacgtgaag ttaaaagcgt tgttgaagca gcacttgatc
    25381 tttatcttaa tggttctgtt gacgacgcat accgggtggc ctccatggat gagcaaatta
    25441 accactattt tgaaactatc cgtgaccttg cgactgaaga gattaagaaa aatccagaag
    25501 ccattgttac gggtcgtgat tatttccaag ttatttccta cttggagcgt atcggtgact
    25561 atgctaaaaa tatctgtgaa tgggttgtct actttgaaac aggtaagatt gtcgaactat
    25621 aaaatagttt aattctgtat aaaaaggagc ttgaaatcaa ctgatggctc ctttttttga
    25681 acgaaaaaat atttttgaga taaaaattta aaaaacactt gacaagtaac ttggatatcg
    25741 ttataattat acaaaattaa cagagaggtt gtttatttat gaaatcaaaa aaatggatat
    25801 ttgttttatg taattttctt gcaagtttct tcttagtagc ttgccagtca ggttctaatg
    25861 gttctcagtc agctgttgag gcaattaagc aaaaggggaa attagttgtg gcaaccagtc
    25921 ctgactatgc accctttgaa tttcaatcat tggttgatgg aaagaaccag gtagtcggtg
    25981 cagacatcga catggctcag gctatcgctg atgaacttgg ggttaagttg gaaatctcaa
    26041 gcatgagttt tgacaatgtt ttgaccagtc ttcaaactgg taaggctgac ctagcagttg
    26101 caggaattag tgctactgac gagagaaaag aagtctttga tttttcaatc ccatactatg
    26161 aaaacaagat tagtttcttg gttcgtaagg ctgatgtgga aaaatacaag gatttaacta
    26221 gcctagaaag tgctaatatt gcagcccaaa aagggactgt tccagaatca atggtcaagg
    26281 aacaattgcc aaaagctcaa ttaacttccc taactaatat gggtgaagca gtcaatgaat
    26341 tgcaggctgg aaaagtagat gctgttcata tggatgagcc tgttgcactt agttatgctg
    26401 ctaaaaacgc tggcttagct gtcgcaactg tcagcttgaa gatgaaggac ggcgacgcca
    26461 atgccgttgc tcttagaaaa aatagtgatg atttgaaaga agtggtagac aaggtcatcc
    26521 aaaaacttaa ggatgaagga atttaccaaa gctatcttga aaaagcagca agtctaactg
    26581 aagttgaaga ataagaaaaa gcagagttgg atgtcattcc aattctgctt ttaaatagtt
    26641 taaaacattt tccagattgt ctagggacac cttggcaaat tgataaggac aggtacttaa
    26701 ataggcctct tcaaagaagt ccaagcccgg ttgactgtgt ttgagactgg cgattttagg
    26761 atactgtctc aggtctagca ataaaccatc gtgtagggga taatgaggaa ggggacaagg
    26821 tgttttagtt atcctttcct cgatttgttt ttggtaagat tcatgattgg tcaagcgagc
    26881 caaacgattt ttttcaaaca attgactgtc aatatagagt gacagggcct tttgcataat
    26941 gaggttgctg tcgtagtcta ggatattttt gtaggccaca aatgcttctt tgatagcatt
    27001 tggaagaatg agtgctgtaa tacgaagggc aggaaaaagg ctggtcgaga aggacttgat
    27061 ataaatgaca tgctcctctg tatcaagata gtggaaggtt tggcccttct tggagtccaa
    27121 atcacccaga taatcgtctt ctacgatata gacatcatac ttggcagcta agttaagaat
    27181 ggatcgtttg tcttgctcag aataggaatg tcccagagga tagtgaaatc ggggaatggt
    27241 gtagaaaaac ttaatttttc ctgtcttgaa gtgtcgctcc agctccttca ggtcaatccc
    27301 atcaatgcct cgttcaatcg tttgatagtc cagcccctgt gcaatcaaga ggcgattcat
    27361 ccgatggtag gttggctgtt ccaccaagat ttccttggct tggctaggaa aggatatttg
    27421 agagaggata aacaaggctt gttgggttcc agaagtcagt actagttggt tagccttgca
    27481 gtagagagct tgctcaaaga ggagtttgtg aatggattgt cttaggtctt ctaatccttc
    27541 ttgattatca tagtagttga agaggtagtt ttctcggcca atcaaggttt cattgacaca
    27601 gagtcggaaa tcgtcatagg cactggcatg ttcgtcggta acctcgattt ctaggtcctg
    27661 gtgttgccct tgttctagga catagtagcc actctggggc ttggcataga ggtattgttc
    27721 gtgccgtaat tccagcaggg ctcgttgaat ggtgtccttg ctacagtgaa agtcaaggct
    27781 cagttgacgg atagaaggca ggcgactacc cgtcggaaat cgtccagact cgataccatt
    27841 tttcagatag gaaacgacct ctttgtactt gctttgtttc ttcattccag acctcccttg
    27901 attttgttac attgtaccct tttttttcct tcttggcaat gtctagagcg tttctctcgt
    27961 tcacatctag gggcaaatgt ggtatactta ggagtaagat ttatagaata acagacaaga
    28021 aagtgaatgg ataagaacgt gcaggaacca gtgaaattat ttcaatacaa tactcttgga
    28081 gccttgatgg ctggccttta tggtggtacc atgacagtgg gagaattgct agagcatggt
    28141 gaccttggtt tgggaacctt ggattctatt gatggggaat tgattgtctt ggatggcaag
    28201 gcttatcagg ccaaagggtc aggagaccag ccagaaattg tggaagtgtc accagatgcc
    28261 cttattcctt acgctgcagt ggtaccgcat caggcagagg tcattttccg ccagcgcttt
    28321 gagatgacag acaaggaatt gaaagaacga attgagtctt attatgatgg ggaaaatctt
    28381 ttccgttcta tcaagattcg gggagaattt tcacatatgc atgtgcgtat gattcctaag
    28441 tcaacacccg atactaagtt tgctgatgtc gcaacccatc aaccggaata tagtcgtgac
    28501 aatgttgcgg ggactattgt tggtttctgg acgcctgaga ttttccatgg ggtcagtgtg
    28561 gcaggctacc atctgcactt catatcagat gatttgacct tcggtggaca tgtcatggat
    28621 tttgtcatca aggaaggcat tatcgaggtg ggagcagttg accagttgga ccaacgtttc
    28681 ccagtccaag accgtcaata cttgtttgct aagttcaatg ttgacgagat gaaaaaagat
    28741 attgaaaagg cagaatagga gaagaaaatg accattcata tcattattac catgttgctg
    28801 ttgctagctt ttctgatagg aagcatttgg tttgccaaaa agaaatacca gattaatcta
    28861 gctgtcttgg gcttgggtgc agtcgctttc tttgtctctt cgcagatttt ggaaaaactg
    28921 gtgcatatct tgattttgca ccctcaaaaa gacggtagta ttgccctctt gcaagatcat
    28981 ccgcttatct atattatcta tggcctagcc atggcagcat tttttgagga aacggctcgt
    29041 cttgttttct tcaaatggtt ggagaaaaag agaagtttgg aaaaggcaga tgccttggcc
    29101 tatggcctag ggcatggtgg cttggagttg atttttctag gccttattag tttactcaat
    29161 ctttacatcg ttctttcggc agttcaaact cagaatccac aggttatgca attactgtct
    29221 gaaaatatgt taaaaaccat tcagtcactt tcggtctggc agatttattt gcttggtttt
    29281 gagcgaatct tggcgcttgg tttccaatta cttttgacag tttgggttta ccaagctgtt
    29341 cgccagaaga aatggattta tctcctagca gcctatggct tgcatgcctt ctttgatctg
    29401 gcaccatctc ttttccaagt aggctggttg acaaatccag tcttggttga agtgattcta
    29461 gcactggagc tcgttctggt cgcctatgga accaaggaaa tcttttgtaa aaaatcataa
    29521 aaagggggga acctcttttt cttatgcaaa atccaaacaa ggtattttta tggtcgtcaa
    29581 atgtctctaa aaatggtata atggaatgaa ttttgtaaaa ggaagagtga catgtctgta
    29641 agagaaaaaa tgcttgaaat cttagaagga attgatatcc gttttaagga acccttgcat
    29701 agctatagtt atacaaaagt aggtggagag gctgattatt tggtctttcc acgaaatcgt
    29761 tttgagttgg cgcgccttgt gaaatttgcc aaccaagaaa atatcccttg gatggttctt
    29821 ggcaatgcaa gcaatatcat cgttcgtgat ggtgggattc gtggatttgt catcttgtgt
    29881 gacaagctca ataacgtttc tgttgatggc tataccattg aagcagaagc tggggctaac
    29941 ttgattgaaa caactcgcat tgccctccgt catagtttga ctggctttga gtttgcttgt
    30001 ggtattccag gaagcgttgg cggtgctgtc tttatgaatg cgggtgccta tggtggcgag
    30061 attgctcaca tcttgcagtc ttgtaaggtc ttgaccaagg atggagaaat cgaaaccctg
    30121 tctgctaaag acttggcttt tggttaccgc cattcagcta ttcaggagtc tggtgcagtt
    30181 gtcttgtcag ttaaatttgc cctagcccca ggaacccatc aggttatcaa gcaggaaatg
    30241 gaccgcttga cgcacctacg tgaactcaag caacctttgg aatacccatc ttgtggctcg
    30301 gtctttaagc gtccagtcgg gcattttgca ggtcagttaa tttcagaagc tggcttgaaa
    30361 ggctatcgta tcggtggcgt agaagtgtca gaaaagcatg caggatttat gatcaatgtc
    30421 gcagatggaa cggccaaaga ctacgaggac ttgatccaat cggttatcga aaaagtcaag
    30481 gaacactcag gtattacgct tgaaagagaa gtccggatct tgggtgaaag caagtaggaa
    30541 gctgtatttg aaaagctgct gctatgtcat ggaaaggggg tgaaagccta tcggtagcga
    30601 agatgtatgc aggtggtttt actccctgca agaggtagtg gggacctgac agagccccga
    30661 tcggttaatc tatgaaaaag aaggaattta tgccaattga aaaaaccaat tatcgaattc
    30721 aaaaacgtct ctaaagtttt tgaagacagc aacaccaagg ttctcaaaga catcaacttt
    30781 gagttggaag aagggaaatt ctacaccctt ctaggtgcat ctggttcggg gaaatcaact
    30841 atcctaaaca ttattgcagg tttactggat gcgacgacag gagatatcat gctagacggt
    30901 gttcgtatca atgatattcc aaccaacaag cgcgacgtac ataccgtctt ccaatcctat
    30961 gccttgttcc cacatatgaa tgtgtttgaa aatgttgcct ttccacttcg cttgcgtaaa
    31021 attgataaga aagaaatcga gcagcgtgta gcggaagttc tcaagatggt tcagttggaa
    31081 ggttatgaaa aacgttccat ccgcaaactt tctggaggac aacgtcagcg tgtggccatc
    31141 gcccgtgcta tcatcaacca accccgtgtg gtcttgttgg acgagccttt atcagcgctg
    31201 gacttgaaat tgagaacaga catgcagtac gaattgcgtg aattacaaca acgattgggc
    31261 attacctttg tctttgtcac tcacgatcag gaagaagctc ttgccatgag tgactggatt
    31321 ttcgttatga atgatggcga gattgtccag tctggaaccc ctgtggacat ctacgatgag
    31381 ccaatcaacc actttgttgc cacctttatc ggggagtcaa acatcttgcc aggtaccatg
    31441 attgaggact acttggtcga atttaacggc aaacgctttg aagcggttga tggtgggatg
    31501 aagccaaatg aacctgttga ggtcgttatt cgtccagagg acttgcgcat tacccttcct
    31561 gaagaaggca aactccaagt taaggtcgac acccagctct tccgtggagt tcattatgaa
    31621 attatcgcct atgacgaact tggaaatgaa tggatgatcc actcaacccg taaggccatc
    31681 gtgggtgagg aaatcggtct ggactttgaa tcagaagaca tccacatcat gcgtctcaat
    31741 gaaaccgaag aagagttcga tgctcgtatc gaagaatacg tggaaatcga agagcaagaa
    31801 gcaggtctga tcaatgcgat cgaggaggaa agagatgaag aaaacaagct ctaaactctt
    31861 tgtagtgccc tacatgcttt ggattgccct ctttgtattg gcacccttgg tcttgatttt
    31921 cggacaatcc tttttcaaca ttgaaggcca gtttagttta gaaaattata aatcttactt
    31981 tgcgtcacaa aacttgacct acctcaaaat gagtttcaac tcagtgcttt atgcagggat
    32041 tgtgaccttt gtgaccttgc ttatcagcta tccaacagcc ctctttttga cccgtctcaa
    32101 gcaccgtcaa ctctggctca tgctgattat ccttcctacc tggatcaatt tgctccttaa
    32161 agcctatgct tttatcggga tttttggtca aaatggctct attaaccaat tcttggaatt
    32221 tatcggaatt ggttcacaac agttgctttt taccgatttt tcctttatct ttgtcgcaag
    32281 ctacatcgag ctccccttta tgattttgcc gattttcaat gtcttagacg atatggataa
    32341 taatctcatc aatgctagtt acgaccttgg tgcaactaag tgggagacct tccgtcatgt
    32401 catcttccct ctatctatga acggtgtgcg aagtggggtt cagtcggtct ttatcccaag
    32461 tttgagtctc ttcatgctga cccgtttgat tggtgggaac cgcgttatca ccttggggac
    32521 ggctattgag cagaattttc taaccaatga caactatggt atgggttcaa ccatcggtgt
    32581 gattctcatc ctgaccatgt tcatcaccat gtgggtgacc aaggaaagga gagaacgatg
    32641 aaaaaatttg ccaaccttta tctgggactg gtctttctgg tcctctacct gccgattttt
    32701 tacttgattg gctatgcctt taatgctggt gatgatatga atagctttac aggttttagc
    32761 tggactcact ttgaaaccat gtttggagat gggagactca tgctgatttt ggctcagaca
    32821 tttttcttgg ccttcctatc agccttgata gcgaccatta tcgggacttt tggtgccatt
    32881 tacatctacc agtctcgtaa gaaataccaa gaagcctttc tatcactcaa taatatcctc
    32941 atggttgcgc ctgacgttat gattggtgct agcttcttga ttctctttac ccaactcaag
    33001 ttttcacttg gctttttgac cgttctatct agtcacgtgg ccttctccat tcctatcgtg
    33061 gtcttgatgg tcttgcctcg actcaaggaa atgaatggcg acatgattca tgcggcctat
    33121 gacttgggag ctagtcaatt tcagatgttt aaggaaatca tgcttcctta cctgactccg
    33181 tctatcattg caggttactt catggccttc acctattcgc tggatgattt tgccgtgacc
    33241 ttctttgtaa caggaaatgg cttttcaacc ctatcagtcg agatttactc tcgtgctcgc
    33301 aaggggattt ccttagaaat caatgccctg tctgctctag tctttctctt tagtattatc
    33361 ctagttgtag gttattactt tatctctcgt gagaaggagg agcaagcatg aaaaaaatct
    33421 attcattttt agcaggaatt gcagcgatta tccttgtctt gtggggaatt gcgactcatt
    33481 tagatagtaa aatcaatagt cgagatagtc aaaaattggt tatctataac tggggcgact
    33541 atatcgatcc tgaactcttg actcagttta cagaagaaac aggaattcaa gttcagtacg
    33601 agacttttga ctccaacgaa gccatgtaca ctaagataaa gcagggtgga acgacctacg
    33661 atattgccat tccaagtgaa tacatgatta acaagatgaa ggacgaagac ctcttggttc
    33721 cgcttgatta ttcaaaaatt aaaggaatcg aaaatatcgg accagagttt ctcaaccagt
    33781 cctttgaccc aggtaataaa ttctccatcc cttacttctg gggaacctta ggaattgtct
    33841 acaacgaaac catggtagat gaagcgcctg agcattggga tgacctttgg aagccggagt
    33901 ataagaattc tatcatgctc tttgatgggg cgcgtgaggt gctgggacta ggactcaatt
    33961 ccctcggcta cagcctcaac tccaaggatc tgcagcagtt ggaagagaca gtggataagc
    34021 tctacaaact gactccaaat atcaaggcta tcgttgcgga cgagatgaag ggctatatga
    34081 ttcagaataa tgttgcaatc ggcgtgacct tctctggtga agccagccaa atgttagaaa
    34141 aaaatgaaaa tctacgttat gtggtaccga cagaggccag caatctttgg tttgacaata
    34201 tggtcattcc caaaacagtt aaaaaccaaa actcagccta tgcctttatc aactttatgt
    34261 tgaaacctga aaatgctctc caaaatgcgg agtatgtcgg ctattcaaca ccaaacctac
    34321 cagcgaagga attgctccca gaggaaacaa aggaagataa ggccttctat cccgatgttg
    34381 aaaccatgaa acacctagaa gtttatgaga aatttgacca taaatggaca gggaaatata
    34441 gcgacctctt cctacagttt aaaatgtatc ggaagtagga gataaacgta agaagacgaa
    34501 tcagtcaagc tggttcgttt ttaaggaata gaagtcagtt tttagagccc tatctttttc
    34561 tattctagtt gtttatttgt ttatttgctt atttgcttgc ctagtagtat actaagccta
    34621 tactcttcaa accacgtcag cgttgccttg ccgtactcaa gtacagcttg aggctagctt
    34681 cctagtttgc tttttgattt ttattgagta ttaaccttac agaaagcggc aacaaataat
    34741 gaaaatgtct acttttttca aaaaaagttt ctggccaacc tttatgattg ttaaccaaac
    34801 tgttatacta ttccatttaa aggatggctt ggatcgacaa tacctaacaa ctgagtccat
    34861 atattgggtg ataggcacct ttatatttgg aaatattcta gtagctgtgt ttagtaatat
    34921 gaaaatttgg gataaaaaga aaaatggcag taagaaaaaa tatatcctga aaaagtaatc
    34981 agagatgtag ggaattggtc taattcaacg gagatcattt ccagttgacg caagattaaa
    35041 aaaacgatat aataagaaag ttgagaattg attttcactt gtcttagtcc agagaaatgg
    35101 cggtgctgcg agccatctaa gctaggaagt catgctactc aatcatacaa ttgcataaga
    35161 acaagagaat gacaagttca ttgaatgaag gtggtaccgc ggtttttcgc ccttcgtgat
    35221 atgagcttgt cttttgattt ttggaggtgt tgatgaaaac atttcttgtc aaacaaaagt
    35281 ttcgtcttgg aggcgaacgc ttcgctatca agaatgacag gggagaaatt gcctatcagg
    35341 tggagggatc attttttaag attcctaaaa cctttaccat ctatgatgcg gctggtgaac
    35401 aagtcagtga gattagtaaa gaaatcttga ccttgcttcc tcgttttgag attcagcttc
    35461 gggatggctc gagttttgtc attcgtaaga agttgacctt ctggcgagat aagtatgagt
    35521 ttgataatct aggtcttcgt atcgagggca atatctggga tttgaatttc aaattgctgg
    35581 atgatcgcga tcagttgatt gcggaaatta agaaggaact cttccatctg acctctacct
    35641 ataccgtaac ggttcttgaa gacgcttatg cagacctagt catttccctc tgtgtcgcga
    35701 ttgactatgt ggaaatgctg gaaagccaat cacattaaac aagtgaataa ggagacaata
    35761 tgaaacaact atctagtgca caagtacgcc aaatgtggct tgatttctgg gcgaccaaag
    35821 gtcactcagt agaaccatca gtaagtttgg ttcctgtaaa tgacccaact cttttgtgga
    35881 tcaactctgg ggtagcaacc cttaagaaat actttgacgg gaccattatc cctgaaaatc
    35941 catgtattac caatgcccaa aaggctatcc gtaccaacga catcgaaaac gtagggaaga
    36001 ccgcgcgtca ccataccatg tttgaaatgc tggggaactt ctctatcggg gattacttcc
    36061 gtgacgaagc tatcacttgg gcttatgagc ttttgacaag ccctgaatgg tttgatttcc
    36121 ctgctgaaaa actttacatg acctactatc cagacgataa agattcttac aaccgctgga
    36181 ttgaagtggg agtggatcca agtcacttga ttccaattga ggacaacttc tgggaaatcg
    36241 gtgcgggacc ttctggacca gatacagaaa tcttctttga ccgtggggaa gcctttgacc
    36301 cagaaaatat cggtcttcgc ctgcttgcag aagatattga aaacgaccgt tatattgaaa
    36361 tctggaacat cgttttgtca caatttaacg cagaccctgc tgttcctcgt agcgaataca
    36421 aggaattgcc acataagaac attgatacgg gcgctggttt ggagcgtttg gtggccgtta
    36481 tccaaggggc taagaccaac tttgaaacgg acctcttcat gccgattatc cgtgaagtcg
    36541 agaaattgtc tggtaaggtt tatgaccaag atggcgacaa catgagcttc aaggttatcg
    36601 cagaccacat tcgttctctt tcatttgcta tcggtgatgg tgcccttcca ggaaatgaag
    36661 gtcgtggtta tgttcttcgt cgtcttctcc gtcgtgcttc tatgcatggt caaaaattgg
    36721 gtatcaacga gcctttcctt tacaaattgg ttccaactgt tggaaaaatc atggaaagct
    36781 actacccaga agtgcttgag aaacgtgact ttattgagaa aatcgttaag agcgaagaag
    36841 aatcatttgc ccgtaccctt cactcaggtc aacactttgc ccaaggcatt gtagcagact
    36901 tgaaagaaaa aggtcaatct gttatcgctg gttcagatgt cttcaaactc tatgatactt
    36961 atgggttccc agttgaattg actgaagaaa tcgctgaaga agctggtatg actgtagacc
    37021 gtgaaggttt tgaagcagcc atgaaagaac agcaagaacg cgcgcgtgcc tcagctgtca
    37081 agggtggctc aatgggtatg caaaatgaaa ctcttcaaaa catcactgta gaaagtgtct
    37141 tcaactacaa tgctagccaa ttgtcttcta aattggtagc tatcgtggcg gacaatgcag
    37201 aagtagaagc tgtttcagaa ggaactgcct ctcttatctt tgcggaaacg tcattttatg
    37261 ctgaaatggg tggacaggta gctgactacg gacaaatctt ggatgagtca ggtaaggttg
    37321 tggctactgt gaccaatgtt cagaaagccc caaatggtca agcccttcat acagttgaag
    37381 tccttgcacc gcttgccttg aaccaagaat ataccttggc aattgatagc aatcgccgtc
    37441 accgtgtcat gaaaaaccac actgcgactc atttgcttca cgctgccctt cacaatatcc
    37501 ttggaaacca cgcaacacag gcaggatctc ttaacgaagt tgaattcctt cgctttgact
    37561 ttacccactt ccaagcagta actgctgagg aacttcgtgc gattgaacag caagtcaacg
    37621 aaaaaatctg ggaagctctt gaagttaaga cagttgaaac ggatattgac actgctaaag
    37681 aaatgggagc gatggccctc tttggtgaga aatacggcaa ggaagttcgt gtcgtgacta
    37741 tcggtgacta ctctattgaa ctttgtggtg gtacccacgt tggcaacact tctgagattg
    37801 gtctcttcaa aattgtcaaa gaagaaggaa tcggttcagg aactcgtcgt atcttggcag
    37861 tgactggtaa ggaagccttt gaagcctatc gtgaacaaga ggacgctctt aaagctgttg
    37921 cagcaacctt gaaagcacct caagtcaagg aagtacctca caaggtagaa ggacttcaag
    37981 aacaacttcg tcaacttcaa aaagaaaatg ctgagttgaa agaaaaagcc gcagctgcag
    38041 ccgcaggcga tatcttcaag gatgtgaagg aagtcaacgg tcaccgctac attgctagtc
    38101 aagtgtctgt atcagatgcc ggtgcccttc gtacctttgc agataactgg aaacaaaaag
    38161 actactctga tcttcttgtc ctagttgctg ctatcggtga caaagtcaat gtccttgtag
    38221 caagcaagac aaaagacctt catgcaggaa accttgtcaa agaattagca ccaatcatcg
    38281 atggacgtgg tggtggtaaa ccagacatgg ccatggcagg aggaagcaac caagctaaga
    38341 tccaagaatt gttggatgca gtagcaggta aattgtaatc aagtaaagat ctatccattt
    38401 gggtaggtct ttttgtgaat acaaaaaagc caaatccgct tggacctagc ttggttatta
    38461 tgagctatta gatggtattg actgcccaga cacttacgga tctagctgag acagggaatt
    38521 gtccataacc ttcctcatca attgtaactt gaccttggtg gttaccaagt aaatctacaa
    38581 aggtttgatt agtccattct tgaccgacaa acattgactt gctgttttct tggtcatttg
    38641 agataaggac tgcgattggg gattgatttt cagcacctga acgtacccaa ccgatacagt
    38701 tagcatggtc aaagtagtca ttttgttctc cataggccaa atcttttcgg atggctagga
    38761 ggcggtcaag gatttctttg aaatcttgtt gagcatactg ccctgaaatc ccatagtagt
    38821 ctccgtaaaa gacacatgga aggccgtctt ggcgtaacaa aatgagggca taggctgctg
    38881 gcttgaacca ttcttcaacg gtagactcaa gggcttgtcc tcgttgggta tcgtggttgt
    38941 cgacaaaagt cacagccttg tcaggcttga gttcaaccag gctatctgtg aaaatgccac
    39001 gaaggtcata gtttgcgcca gcttgactgg cttcaaagag attctggtgg agacgaacat
    39061 cgacaaggtc aaagtgttct tccgtttttt cgagatagtc cagattggct tccttgtctg
    39121 ggttccaaaa ttcaccaaaa acatagaaat cgtcaccgta tttttccttc atatcgcgga
    39181 tgaagttgcg cataaagaaa gagtcaatat gcttaacggc atccaaacgg aaaccagcta
    39241 caccagtcgt ttccatgaac caatcagccc agtcatagat gttttggatg acttcaggat
    39301 gtttaaagtc taggtcggca tacatgaggt agtcgtagtt tccgttttcg ttatcgacca
    39361 attcctcgtt agcccagccc ttgttgtccc cttggatcag ataaatccca gatttactgc
    39421 gtttggcatc gtagtctgta ccggtgaagt ggtaccaatg ccagtggaag ccattatagg
    39481 tatcttggcg accatcgaag gtaaaactag tccagccatt gatggtgaag ggttctccaa
    39541 gttcaactgt acggtctaca ggatcaactt cgatgacctg aaaggcttcc ctgtgatcgg
    39601 cagcagcctt gtggttgaga actacatcgg ccataggttg aattccctgt gctttaaggg
    39661 cttgaatggc ttgaagatag tcttctttga aaccatactt ggtgcggaca gtcccttttt
    39721 ggttgaactc tcctaagtca aataagtcat agaccccata gccgacatct ttttcgttgg
    39781 ttgccttgaa ggctggtggc atccagacgt gactgatccc cagatgagct aggtgtggag
    39841 cattttcagc cagacgcgtc cagtgttgac cgtcgtgggg cagataccat tcaaagtatt
    39901 gcatgagtgt ttgattttgc atgatgtttc ctcttacttg tcaatcttgt tctttattct
    39961 atcataaagt atctcttggt gcaaacgttt gcgtaagata gaaggaaact attttaacta
    40021 cttaaaataa agtattgatt tttttgtatc aaagtgctag tatagcgatg tataatacag
    40081 ataaagatag gagttgtccc atgattgaat ttcaaaatgt tagtaagttg tatggtgata
    40141 aagaggcctt gagtaatctc aatttgcaga ttgaaaatgg agagattatg ggcttgattg
    40201 gtcataatgg ggctggaaaa tcgaccacca taaaatccct agtcagtatc atttcaccca
    40261 gcagtggtcg tattttggta gacggtcagg agttatcgga aaatcgcttg gctattaaac
    40321 gaaagattgg ctacgtagca gactcgcctg acttattttt acgcttaacg gccaatgaat
    40381 tttgggaatt gatcgcctca tcctatgatc tgagtagatc tgacttggag gctagtctag
    40441 ctaggctatt gaacgttttt gattttgctg aaaatcgcta tcaggttatt gaaactcttt
    40501 ctcacggaat gcgtcagaaa gtctttgtca tcggagcact cttgtctgat cccgatattt
    40561 gggtcttgga tgaacccttg actggtttgg atccccaggc tgcctttgat ttgaaacaga
    40621 tgatgaagga acatgcacaa aaagggaaga cagtcttgtt ttcaactcat gtcctagagg
    40681 tggcagagca agtctgtgat cggattgcca ttttgaaaaa ggggcatttg atttattgtg
    40741 gtaaggtaga ggacttgagg aaagaccacc cagaccagtt tttggaaagt atctacctta
    40801 gtcttgctgg tagaaaagag gaggttgcgg atgcgtctca aggtcattaa aaaattagtt
    40861 gatatcaata tcctctattc atctcaagaa gctaatctgg ctaatctacg aaagaagcag
    40921 gctaagaatc ctgggaaaaa agtaaatgtt tctgctagag tcctaagttc ttacattttt
    40981 tccagtctct tgatgctcat ctgttttagt aatatagcca ttcattttcc ttttgaggaa
    41041 atgcctattt attttagttc gatggttgct attttactgg tgctggcctt ttcaacttct
    41101 ttcactgcct tttacaatgt cttttatgag agtaaggacc tggcctccta taggccctat
    41161 gcctttaaag aatcagagat tataatggcc aaaggtctgt ctgtcctctt gccagctcta
    41221 actggaattg taccaatcct agcttatttt ctagccctct atattagtct agctccttct
    41281 ctttggctgg gtttgccttt gatgctactg tccttgacct tattatttgt ctctgttact
    41341 ttagtgacgg tagtggcagt acatttcttg gctcagacta gggtcttcag aaagtatcag
    41401 tctatttttt cgaatgtgat gattgggata ggagttctca tacctttaat atttatcttc
    41461 tttcttcagt cgacttttgg aagtattgtt gacaaagtta gagacattcc atttctcctt
    41521 tatcctcttc atatctttta caaaatagca gtggagcctt tttcgacaga agccttagtg
    41581 ggtctgctcg cttggatagg actaactctc ttcctgcttt atctgaccaa aaagaaggtc
    41641 cttcctcgtt tttatgacgt gatcctgctt aacagtgagg agaaggtcaa aaaagaacgt
    41701 cgcagcaagg agaggatttc aactactaaa aagggctttt tccgtatggt tttacgctac
    41761 cacctcaccc tcttgggaca ggggactggt gtgattacag tgctttttac aagtgctttc
    41821 cttccttatc tcatgatgat cagtctgatt tccaaaatcc gagattctca gatagttcca
    41881 gacattcatc ctccatactg gttacccttg ttttttgtag gagtgtttat agcagttgtc
    41941 aataacaata tcaccagcct gcattcaatt gccttgtcct tggagaggga aaatgttgat
    42001 tttctgaaga gtttaccctt tgactttgct cgttatgtga aagtgaaatt ttggattatc
    42061 tttgctgttc agtccttttt accagttctg actttgctcg gtctttctct atatctaggc
    42121 ttgtccatcc tttcgatgat ttaccttctt gtagtctgga tcattgccag tgtcatcctt
    42181 tcttgccacc attactttaa ggacgttaaa aatctgtcaa ccaattggag tagcattacg
    42241 gacctggtga atcgttcaaa tggcatagtc gccatagttt tattgtttat ttatagtgca
    42301 atcctgatgg cccttgtaat tgggagcata ttcttggttc agtctctctc ccctatcctt
    42361 gccatcaact tgggagtagg agctcttatc gtcttgcttg ctcttgctat ttttggctat
    42421 cattattacc tgtcacgcat attggcagaa atagaaaaaa gatgagatag ttggttgctt
    42481 gcttgataaa ttcaattcga caaacgcttt tatagtttgt tagaatgtag ttgtaaatga
    42541 ttaagattgg taatcaaaaa tatagggagg aatgttttga ataagaaata taatatagtt
    42601 ctattattgt tgtttatagt ttatttattg ggatattttt caatttcaaa aacgttaatt
    42661 cctataatgt gtgtgatcca agtatttttg atagaacata tatttagagt tcgaaataga
    42721 atgatgcaaa taggtgaaat tttaattatt gttgcttcta taatattatt tattgatagt
    42781 atattgagtt tgtaacaaaa ggcttctcta taaaatcaaa ttttccagat acctactgaa
    42841 gctgatttat ttcttcatct ttcttctact acctttgtca ctaaatttga cagacacata
    42901 ttgctgatac aacctctcta cctataatct cacggttaac ctgaggctag tttcatagtt
    42961 tgctatttga ttttcgttga taaaaagaaa tgctcctcca agacggaaga gcatttttta
    43021 ttgtgattct tacacattta tttcctagtt ttgcaggaag tctagtatac cattttttaa
    43081 aagaagagta aaaaagcaag ggtagtttct tttttgagaa ttgtctaaaa gtttgatata
    43141 atggtagtta tgaatagaat aagagtcagc aaaagggttg aaaagaagct tgctaagggg
    43201 ctagttttac tagaagccag tgatcttgag aatgtcaatc ttaaggatca ggaagtagag
    43261 gtgcagggtc aggaaggaaa ctttcttggg actgcctacc tttctcagca aaacaagggc
    43321 ttgggctggt ttgtcagcaa agacaaggtg gccttcaatc aaactttctt tgaaacgttg
    43381 tttagaaaag ccaaagaaaa gagaaacgcc tactatcaag atgatttgac aactgccttt
    43441 cgtctcttta atcaagaggg agatggcttt gggggtctga cagtggacct ttatggcgac
    43501 tacgccgtct tttcttggta taactcttat gtttatcaga ttcgtcagac tatatcagaa
    43561 gcctttagac aggttttccc tgaggtttta ggggcttatg agaaaatccg ctttaagggt
    43621 tttgactatg aatctgccca tgtttatggt caagaagcac ctgacttttt cactgtttta
    43681 gaaaatggtg tcctgtatca agtctttatg aatgatggct tgatgacagg aattttccta
    43741 gaccagcatg aggttcgcgg tagtttagtt gacggcttgg ctatgggtaa atccttgctc
    43801 aatatgtttt cctacacagc ggctttttca gtagctgcgg ccatgggagg agctagccag
    43861 acaacttctg ttgatctagc caaacgttca cgagaattgt ctcaagcgca ttttcaggca
    43921 aatgggctca gcacagacga gcatcgtttt atagtcatgg atgtctttga gtatttcaaa
    43981 tatgccaaac gcaaagactt gacctacgat gtgattgtcc tagatccgcc tagctttgct
    44041 cggaataaaa aacaaacttt ctctgtggcc aaggattatc acaagttgat ttcccagagt
    44101 cttgagattt taaatccggg agggattatc attgccagta ccaatgctgc caatgtttcc
    44161 cgtcagaaat ttacagaaca aattgataaa ggctttgcag gaagaagtta ccagatttta
    44221 aacaaatatg gtcttccagc agattttgcc tataataaaa aagatgaaag tagtaattac
    44281 ctcaaggtga ttagtatgaa ggttagtaaa tgaaattaat cgtttcagta atgccaagaa
    44341 gtttagagga ggctcaggct ctggatgcca cgaggtacct ggatgccgac atcattgaat
    44401 ggcgtgccga ctatctgcct aaagaagcga ttttgcaggt agctccagcc atttttgaaa
    44461 aattcgcagg ccgtgagttg gttttcacgc tacgaactcg ctccgaaggt ggagaaatcg
    44521 acctttctcc agaagagtat atccatctaa tcaaggaagt tgcgcaattc tatcaaccag
    44581 actatattga ttttgagtac tatagctaca aggatgtttt tgaggaaatg ctggacttcc
    44641 caaatcttgt tttgagttac cataatttcc aagaaacacc tgagaacatg atggaaatct
    44701 tgtcagagtt gacgatccta aatccaaaac ttgttaaggt tgcggtgatg gctcacacgg
    44761 agcaggatgt cttagacttg atgaactata cacgaggctt taaaaccctc aatcctgaac
    44821 aggaatatgt gaccatttct atgggtaagg tgggcaaggt ctctcgtatc actgcggatg
    44881 tgactggttc gagttggtct tttgccagtc tggatgaggt tagtgcccct gggcagattt
    44941 ctctagctag catgaaaaaa atcagggaga ttttggatga agcttgatgg ctatacacgt
    45001 ttagctgcag ttgttgccaa tcctattaag cattctattt ctcccttcat ccacaataga
    45061 gcctttgagg cgacagctac caacggtgct tatgtggctt gggagattga agcgagtgac
    45121 ttggcagaaa cagtggccaa tattcgtcgc taccagatgt ttggcatcaa tctgtccatg
    45181 ccctataagg agcaggtgat tccttatttg gataagctga gcgatgaagc gcgcttgatt
    45241 ggtgcggtta atacggttgt caatgagaat ggcaatttaa ttggatataa tacagatggc
    45301 aagggatttt ttaagtgctt gccttctttt acaatttcag gtaaaaagat gaccctgctg
    45361 ggtgcaggtg gtgcggctaa atcaatcttg gcacaggcta ttttggatgg cgtcagtcag
    45421 atttcggtct ttgttcgttc cgtttctatg gaaaaaacaa gaccttacct agacaagtta
    45481 caggagcaga caggttttaa agtggatttg tgtgctttag agaatgtttc tgaactgcaa
    45541 gcaaggattg ccgagtcgga tttgctagtt aatgccacca gtgtgggcat ggatggccaa
    45601 tcctctcctg ttcctgaaaa catagtctta ccagaaactc tcttagttgc agatatcata
    45661 taccaaccct ttgaaacacc gtttttgaaa tgggctagaa gtcagggcaa tccagcagtc
    45721 aatggtctgg gaatgttgct ctatcaagct gcagaagctt ttcaactgtg gacaggcaag
    45781 gaaatgccga cagaagagat ttggcagtct ttaacagaaa aataccaata aagaaaggga
    45841 gactctccta tgaaaatcag aatcgatatt cctcatcatc cttatgatat tcagattgaa
    45901 aaaggttgta tggcccaggc tggtcagtgg ttgcgagaac tctggcaacc gcaaaaggta
    45961 gtcattgtga cagataacca tgtagcctct ctctatgcag agaaggtcaa gctcagccta
    46021 gaagatgctg gttttcaggt agctgttttt gatttcttag aaggtgaaga aagaaagaat
    46081 ttaactactg ttcagaaagt ctatgaattt ctagtcaagc aaggtctgac tcgtagcgat
    46141 ggaatcgttg ctcttggtgg tggcgttgtt ggggacctgg ctggatttgt agcctctacc
    46201 tatatgcggg gtattcactt tgttcagatt ccgactagtt tgacagctca ggttgattct
    46261 tctatcggtg gaaagacagg cgttaacaca ccatttgcta aaaatatggt ggggaccttt
    46321 gcccaaccag atggggttct gattgatcca cttgttcttg aaaccctcgg aaaaagagag
    46381 ttgattgaag ggatgggtga agttatcaag tatggcttga ttgaggatcc agaactgtgg
    46441 gctctcttga cggaactgaa tggttctgtt gagagcattt tggaacatgc agagaccttg
    46501 attgaacatt cttgtcaggt gaagcgcaag atggtggttg aagatgagtt ggacaatggt
    46561 attcgtcttt acctcaattt tggccacact attggccatg ccatcgaagc gactgccggt
    46621 tatggcaagg tcatgcatgg agaggctgtt gccatgggaa tggtacagat ttccaaggtt
    46681 gctgaggaaa aaggcctcat gccagctggc attacccaat ctatcacaga gatgtgtcag
    46741 aaattcggct tgcctgttga ctatgaaaat tgggaagttg acaagcttta tcaggctctt
    46801 actcatgaca agaaaacgcg tggtaacacc ttgaaattgg tcttggtgtc agagcttggt
    46861 tcagcgatca ttcacccagt ttctctggaa gagatgaaag actacttggt aaaataagga
    46921 gagtgtatga gatatttaac tgcaggagaa tcacacggcc cccgtctaac agctattatt
    46981 gagggaattc cagctggact tccattgaca gctgaggata tcaatgagga ccttagacgc
    47041 cgtcagggtg gctacggtcg tggtggtcgt atgaagattg agagtgacca ggttgtcttt
    47101 acttcgggcg ttcgccacgg gaagacgaca ggggcgccta ttactatgga tgtcatcaat
    47161 aaggaccacc agaaatggct ggacatcatg tctgcggagg acattgaaga ccgccttaaa
    47221 agcaagcgga aaattactca tcctcgccca ggtcatgccg atttggttgg ggggattaag
    47281 taccgttttg atgatttgcg aaattctttg gagcgttcat cagctcgtga aaccaccatg
    47341 cgggtggcag ttggtgcagt agccaaacgc ctcttggctg agctggatat ggagattgcc
    47401 aaccatgtcg tggtctttgg tggcaaggaa atcgatgttc ctgaaaatct gacagtcgct
    47461 gaaattaagc aaagagctgc ccagtctgaa gtttctattg ttaaccaaga acgagaacag
    47521 gaaatcaagg actatattga ccaaatcaaa cgtgatggtg ataccatcgg tggggttgtg
    47581 gagacagtcg tcggaggcgt tccagttggt cttggttcct atgtccaatg ggatagaaaa
    47641 ttggatgcaa gattggctca agctgttgtc tctatcaatg cctttaaagg ggtggaattt
    47701 ggtcttggct ttgaggctgg ttatcgtaaa ggcagccaag ttatggatga aattctctgg
    47761 tctaaagaag acggttatac tcgccgtacc aataatctag gtggttttga aggtggtatg
    47821 actaatgggc aacccatcgt tgttcgtggg gtcatgaaac ccattcctac tctttataaa
    47881 cctcttatga gtgtggatat cgaaacccac gaaccttaca aggcaaccgt ggagagaagt
    47941 gatccgactg ctcttccagc tgcaggaatg gtcatggaag cagttgtagc aacggttctg
    48001 gcgcaagaaa tcctcgaaaa attctcatca gataatcttg aggaactaaa agaagcggta
    48061 gccaaacacc gagactatac aaagaactat taaggagttc ctatggcaaa aacaatctat
    48121 atcgcaggtc ttgggttgat tggagcctct atggcacttg gtatcaaacg cgatcatcca
    48181 gattatgaaa ttttaggtta taatcgtagt caagcttcga gagatatcgc cttgaaagaa
    48241 ggcatgattg accgtgcaac ggatgatttt gctagttttg ctcctttggc agatgtcatt
    48301 atcctcagct tgccaatcaa acaaactatt gctttcatta aggagttggc caatttggat
    48361 ttgcgagaag gcgttattat ttcagatgct ggttcgacca agtcaaccat tgtggatgcg
    48421 gcggagcagt atttggctgg caagtctgtt cgctttgtcg gggcccatcc catggctggt
    48481 agtcacaaga caggggctgc ttcggcagat gtcaatcttt ttgaaaatgc ctattatatc
    48541 tttacacctt caagcctgac aagtcaggac acgcttaagg aaatgaagga tctgctttca
    48601 ggtcttcatg ctcgttttat cgagattgat gccaaggagc atgatcgtgt cacttctcag
    48661 attagccatt ttcctcatat tttggcttct agtctcatgg agcagactgc ggtctatgct
    48721 caagagcatg agatggcaag gcgctttgcg gcaggtggtt ttcgagatat gacccgaatt
    48781 gcggaaagcg agccaggaat gtggacctcc attctcttgt ccaatagcga gaccattctg
    48841 gatagaattg aggatttcaa ggaacgtttg gaagtgattg gtcaggccat tagtaaggga
    48901 gatgaagagc aaatttggaa cttttttaac caagcgcgtg aacaacgcca gaccatggaa
    48961 atccataagc gtggtggtgt ggatagctct tacgacctct atgttgacgt tcctgatgaa
    49021 gaagatgtca tcctgcggat tttggaactg ctacgtggga cttccttggt taacatccac
    49081 atcaacgagg aaaatcgtga ggatattcac ggaatcctac aaatttcatt taaaaatgct
    49141 caagacttgg aaagagctga gcatctcata acagaaaata ccgactatac agtcgtcatc
    49201 aaataaggag aagaacatgt caaatattta cgatagtgca aacgaactca gtcgcggtct
    49261 acgcggatta ccagaataca aggctgttaa agcagctaaa gatgcgattg cagcagatgc
    49321 tgaggcaagc aaaatcttta cagaatatct tgcctttcaa gaggaaattc aaaaactagc
    49381 acatacaggt caaatgccag atgcgtcctt ccaagctaag atggaaggct ttggtaaaca
    49441 gattcaagga aatagcctct tgtcagaatt ctttaccaag caacaacaat tggccattta
    49501 cctttctgac attgaaaaaa ttgtttttga accagtttca gaattgctga aataagaaaa
    49561 gaacgatcct aaagtcagga atttttaagg aatttctggc tttttatgat aaaataggta
    49621 aaagtattgc aagtatgagg tccagtatga aactaaaaac aaacattcgc catttacatg
    49681 gtagtatccg cgtcccaggt gacaagtcta tcagccaccg ttccattatc tttggaagtt
    49741 tggctgaggg tgagaccaag gtttatgata ttctgcgagg tgaagacgtt ctttcgacca
    49801 tgcaggtttt tcgtgacctt ggtgttgaaa ttgaggataa agatggggtt attaccattc
    49861 aaggtgtagg catggctggc ttaaaagcgc cacaaaatgc ccttaatatg ggaaattctg
    49921 gcacctcgat tcgcctgatt tcaggtgtcc ttgctggtgc agatttcgaa gtagagatgt
    49981 ttggagatga tagtctttcc aaacgtccta tggaccgtgt gacccttcca ctgaaaaaaa
    50041 tgggcgtcag catttcaggg caaactgaac gagacttgcc tccccttcgc ttaaaaggga
    50101 cgaaaaacct aagacctatt cattatgagt tgccaattgc ctctgcccaa gtcaagtcag
    50161 ccttgatgtt tgcagcctta caagctaagg gggagtcagt tattatcgaa aaagagtgca
    50221 cccgtaatca tactgaagat atgttgaaac aatttggtgg tcatttaagt gtggacggta
    50281 agaaaatcac agtccaaggg ccacaaaaat tgacaggaca gaaggtggtc gtaccaggag
    50341 atatttccag tgcagccttt tggttagtcg caggtttgat tgttccaaat tctcgtctag
    50401 tgctgcagaa tgtggggatc aacgaaactc gcaccggtat tattgatgtc attcgtgcca
    50461 tgggtggaaa attggaaata actgaaatcg atccagtcgc taaatcttct accttgactg
    50521 ttgagtcttc tgacttgaaa ggaacagaga ttggtggcgc tttgattcca cgattgattg
    50581 atgaattgcc cattattgcc ctgcttgcga ctcaagccca aggtgtaaca gttatcaagg
    50641 atgctgagga gctcaaggtc aaggaaacag accgtattca ggttgtggca gacgccttaa
    50701 atagtatggg agcagatatt actcctacgg cagatgggat gattatcaaa gggaaatcag
    50761 ctcttcacgg tgctagagtc aatacgtttg gtgaccaccg tatcggcatg atgacagcta
    50821 tcgcagccct cttggttgca gatggagagg tggagcttga ccgtgcagaa gccatcaata
    50881 ccagctatcc tagtttcttt gatgatttgg agagcttgat tcatggctaa ggtgttatta
    50941 gggtttatgg gggctggaaa atcgactatt gcaagaggct tggaccctaa ttaccttgat
    51001 atggatgctc tgattgagaa gcgcctaggt atgtccattg cgaatttttt cgctgaaaag
    51061 ggagaagcag cctttcgtca ggtagaatca gaagtcctag ctgatttact acaaacagac
    51121 caagtcgtgt caactggagg aggagtggtt atttctcaga gaaatcgtga cttactcaag
    51181 actaatacag ataacatcta cctgaaagca gattttgaaa ccctctacca acgtatcgca
    51241 gctgataagg acaatcagcg accgcttttt ctaaataata gcaaggaaga actagtagct
    51301 atttttcaag aaagacaggc ttggtatgag gaagtggcta gtcgggtttt ggatgtgacc
    51361 aagctaagcc cagaggaaat tatagaggaa ctaagatgaa aattgcttat ctaggtccca
    51421 agggatcatt ttcacaccac gttgtgcaga tagcttttcc tcatgaggaa ttgcaggctt
    51481 ttgctaacat tacagatgtc atcaaggctt atgagcaagg attagtggac tattctgtgg
    51541 tgccagttga aaattctatt gagggtagtg ttcatgaaac cttggactat ctttttcatc
    51601 aggctcacat ccaagcagta gcagaaatcg ttcagcctat tcatcagcag ttgatggtgg
    51661 ttccaggcca tactaagatt gaaaagattt tttcacatcc acaggccttg gctcaaggaa
    51721 agaaattcat cgatgaacaa tatccagagg ctcaaatcga ggtaacagct agtacagctt
    51781 atgcggcccg ttttatttcc gaacatccag accagccctt tgcagcagtt gcacctagaa
    51841 gttctgctga agaatatgga ttggaactga ttgccgagga tattcaggaa atggaagcca
    51901 atttcacacg tttctgggtt ctaggagctg aaaagcctag tattcccttg caagcacaaa
    51961 ctgaaaagat gagtttggcc ttgacattac ctgacaacct tccaggtgca ctttataagg
    52021 ccctgtcgac ctttgcttgg cgaggaattg acttgacaaa aattgaaagt cgtccactca
    52081 agacagcact gggtgaatac tttttcatta tcgatgtgga ttataccgat aaggacttgg
    52141 tccactttgc ccaaaaagaa ttagaagcga ttggaatcca gtataaaatt ctgggtgcct
    52201 atcctattta tccaatatca gaccatggaa aggagagaag atgagtaaag aaaatccctt
    52261 aagtcatcat gagcagttgc gttatgatta tttgctaaaa aatattcact atctcaatga
    52321 gagagaaaaa aatgagtttg cctatttgca agaaaagcta actcttgcta ggggaaatag
    52381 tagctctagc ttggaacaag aaagagaaga gcaggttgac ttaccaagct atgcgaaccg
    52441 gagtcgctca caatccaaat cacaagcact ctctttccct ccaaaaaaga aaagacggaa
    52501 gctccgtctt aaacgaattt ttatggtgat tttttcactt ttggtctgtg tggctttggc
    52561 catggtattc atgtttttgc gtggttacca agatgctagc gcaaagaaaa ctgctgatgc
    52621 ccgggcagct caagtagaag tctttaatgg tcaggacact agagatggag ttaatatttt
    52681 aatcatgggt actgatggtc gaatcggcca gaacagtgtt gagacacgaa ctgactctat
    52741 tatggtatta aatgtcgggg gctcagataa gaaaatgaag ctagtcagtt tcatgcgtga
    52801 taatttggtc tatatagatg gttatagtca agtgattaac ggtagaaaac agacagataa
    52861 caagttaaac gtagcctacg agttaggaga acaagagggg caaaaagggg cagaaatggt
    52921 tcgccaagtc ttgaaagata attttgactt ggacattaag tactacgcct tggttgattt
    52981 ccaggccttt gccacagcga ttgacacgct tttccctgat ggggtgacga ttgatgctca
    53041 attttcaaca ttgaatgggc gtcccctaac agaagctaca gtcggagatg atttatacgc
    53101 taccgagact gagtctccaa cccaaaccat taaagtcgga aaacagcaga tgaatggctc
    53161 gaccctgctc aattatgctc gtttccgtga tgatgatgag gcggattacg gccgtacaaa
    53221 aagacagcaa caagttttaa cagcaatttt agagcaaatt aaagatccca ctaaactttt
    53281 cactggttct gaagctcttg gaaaggtttt tgctatgacc tcaaccaacg taccctatac
    53341 tttcctccta acaaacggtt tatctgtttt ggatggagca aaaaatggta ttgaaaaatt
    53401 gacgattcca gaactgggtg actgggtaga tgcctatgat gtttatggag gcttgggctt
    53461 gctggttgat caaaacaaat atcaaaccaa gctcgctcaa atgggcttga gataagataa
    53521 tactcaatga aaatcaaagt gcaaactagg aagctagccg caggctgcta aaaacacagt
    53581 tttgaggttg tagataagac tgacgaagtc agttacctat acctacggca aggtgaagct
    53641 gacgtggttt gaagagattt tcgaagagta taagacagaa aaatcaaagt ttgatagcta
    53701 tttggatagc agtcaggctt tgatttttta cagtctgcaa tagattttca ccccttgttt
    53761 gtggtataat gaaacgatac gatagaaaga ataatgaaca atatgactga tttaaaagca
    53821 attcaggctc gtagtctgga gatggctgaa tattttgtgg ccttttgtaa agaacatgat
    53881 ttgctctgtt atctctgtgg cggaggcgct attggtgccc ttcgaaacaa gggatttatt
    53941 ccttgggacg acgacctaga cttttttatg cctcgtaaag attatgagaa attagcagaa
    54001 ttatggcctc gttatgcaga tgaacgttat ttcttgtcaa agagtcacaa ggattttgtt
    54061 gatcgtaatc tttttattac cattcgtgac aaggaaacca cctgtatcaa gccttatcag
    54121 caggatttgg atttgccaca tggtctggcc ttggatgttt tgcctttgga ttattatccg
    54181 aaaaatccag ctgagcggaa aaaacaggtt cgttgggcct tgatttattc actcttttgt
    54241 gcgcaaacta ttccagaaaa gcatggtgct ctcatgaaat ggggaagtcg cattttactg
    54301 ggtttgactc caaaatctct ctgttatcgc atctggaaaa aagctgagaa agaaatgact
    54361 aagtatgatt tggctgattg tgatggcatt acagaattat gctcaggtcc tggctacatg
    54421 agaaacaagt acccaatcac atcttttgaa gacaatcttt tcttgccatt tgaaggaaca
    54481 gagatgccta ttccaatcgg ctatgatgtc tatctcagaa ctgcttttgg ggattatatg
    54541 acgcctccac cagcagacaa gcaggtaccg catcatgatg ctgtcatcgc tgatatggat
    54601 aagtcttata cagaatacaa gggagaatat ggtggctaag aaaaaaatct tattttttat
    54661 gtggtctttt tctcttggag gtggtgcaga gaagattcta tcaaccattg tttcaaatct
    54721 ggatccagaa aagtatgata ttgatattct tgaaatggag cactttgaca agggatatga
    54781 atctgttcca aagcatgtac gcattttaaa atcccttcaa gattatcgcc aaaccagatg
    54841 gttacgagct tttttgtgga gaatgagaat ttattttcca agactggctc gtcgtttgct
    54901 tgtaaaagat gattatgatg ttgaagtttc ttttaccatt atgaatccac cactgttgtt
    54961 ctctaaaaga agagaagtca agaagatatc ttggattcat ggaagtattg aagaacttct
    55021 taaggatagc tctaaaagag aatcacatag aagccagttg gatgctgcga atacaattgt
    55081 agggatttca aaaaagacca gcaattctat caaggaagtt tatccagatt atacttctaa
    55141 attacagaca atctacaatg gatatgattt tcagactatt ctagaaaaat ctcaagagaa
    55201 gatcgatatc gagattgctc ctcaaagtat ctgtactatc ggacggattg aggaaaataa
    55261 gggttctgac cgtgtagtgg aagtgatacg attattacac caagagggaa aaaactatca
    55321 tctctatttt atcggggctg gtgatatgga agaggaactg aaaaaacgag tcaaagagta
    55381 tgggattgag gactatgtac atttccttgg ttatcaaaaa aatccttatc agtatctatc
    55441 tcagacgaaa gttcttttgt ctatgtctaa acaagaaggt tttcctggag tgtatgtgga
    55501 ggccttgagt ctgggactcc cttttatctc tacgaacgtt ggaggggctg aggaattatc
    55561 ccaagaagga cgatttggac aaatcattga gagcaatcaa gaggcagctc aggcgattac
    55621 taattacatg acttctgcct caaactttga tgtcgatgag gctagccaat tcattcaaca
    55681 atttacaatt acaaaacaaa tcgaacaagt agaaaaacta ttagaggagt agcatggaaa
    55741 ctgcattaat tagtgtgatt gtgccagtct ataatgtggc gcagtaccta gaaaaatcga
    55801 tagcttccat tcagaagcag acctatcaaa atctggaaat tattcttgtt gatgatggtg
    55861 caacagatga aagtggtcgc ttgtgtgatt caatcgctga acaagatgac agggtgtcag
    55921 tgcttcataa aaagaacgaa ggattgtcgc aagcacgaaa tgatgggatg aagcaggctc
    55981 acggggatta tctgattttt attgactcag atgattatat ccatccagaa atgattcaga
    56041 gcttatatga gcaattagtt caagaagatg cggatgtttc aagctgtggt gtcatgaatg
    56101 tctatgctaa tgatgaaagc ccacagtcag ccaatcagga tgactatttt gtctgtgatt
    56161 ctcaaacatt tctaaaggaa tacctcatag gtgaaaaaat acctgggacg atttgcaata
    56221 agctaatcaa gagacagatt gcaactgccc tatcctttcc taaggggttg atttacgaag
    56281 atgcctatta ccattttgat ttaatcaagt tggccaagaa gtatgtggtt aatactaaac
    56341 cctattatta ctatttccat agaggggata gtattacgac caaaccctat gcagagaagg
    56401 atttagccta tattgatatc taccaaaagt tttataatga agttgtgaaa aactatcctg
    56461 acttgaaaga ggtcgctttt ttcagattgg cctatgccca cttctttatt ctggataaga
    56521 tgttgctaga tgatcagtat aaacagtttg aagcctattc tcagattcat cgttttttaa
    56581 aaggccatgc ctttgctatt tctaggaatc caattttccg taaggggaga agaattagtg
    56641 ctttggccct attcataaat atttccttat atcgattctt attactgaaa aatattgaaa
    56701 aatctaaaaa attacattag aacgggggtg accaagtgat tgatgggaaa cgattattat
    56761 ttagtttgac catagtcagt tatgccttga cgctagtaag tggaattgtg tatctgttta
    56821 ataataataa tgttagctta ctttctactt tattgttctt gttggttagt agcttaattg
    56881 cttgttggaa tgatatcaag tattacttaa tccattttat tttctattta accatttttg
    56941 tatttctggt atcaagaccg accattgatt attttaggga tggtgctttg gatacctatc
    57001 atccaatagc ctatcgtttt gcctttatag ttgtcatggt ttcgattctg ggcttgacca
    57061 caggaggcat tctggctcgt tacttcatag ctaggaagaa aataaaagta gcaaatatag
    57121 gaaattctct aaaagaggtt tatatcaagc ggttacgctt tgtatcacta ggagtttttc
    57181 ttctaaccta tcctttctat ttcattcggt tatttgaacg gctcttgtat cgtttgcaga
    57241 cttcctacta tgcctactat gcaaattttg aaagtaaact gccttatttt acctacattt
    57301 tgtctacctt tacggtctat gcaatgtgta tgtatctggc aaccaagcca aagaaattgc
    57361 aggccacagc agtgcttgtc tcctttattg cagctaatac tattcatttg gcaattggga
    57421 cacgaaatcc ctttatttta agtattttat ttgcttttgt ttattacttt atgcgggagc
    57481 aaactgaaaa aggaaaatgg attgggttta aagaaaagtt agcgattttt gtaggttctc
    57541 ctattctcat gttagcgatg ggagtactca attatgtacg ggataatgtc caagtttctc
    57601 atacaggttt ctgggatatc ttacttgact ttatctataa acaagggact agttttggtg
    57661 ttctggctcg aggttttcta tttaacagta gcctccctta ccgagatttc cgtaatttta
    57721 cttttggtcc tgttcttgat tattttgcaa gggggagttt gggagccatt ttcggaggaa
    57781 aagcctttga acatacaacc aatagtgtgg aactagctat tgatagtaat agttatgccc
    57841 acaatctatc ctatcttgtc ttgaacaagg aatacttgaa agggcatggt atcggaagta
    57901 gttatatcat ggagttgtat accgactatg gtatgattgg agtctttctg cttagtttct
    57961 tactcggcgt attatttata gccatgctgc aagtagccta tcgctcaagg acaatcctat
    58021 ttgctttatc cctactcatc ttgaataatc tattctttat gccaagaagc agcttttcag
    58081 aaagtttctt caatttattt acaatgcaat tctggggaat tgttcttgtg attatatttg
    58141 tagcaaaaat gcttacaaag gaaaactagt atctactaaa caaaggagaa aaaaatcatg
    58201 tttgaacatt attcagtagc tgatttgttt gcaaatcttt ataaaaagcg aaaagcaaat
    58261 attttagctc tcatcgcttt atttgctctc attgctgtac catttacaat taaagcagtt
    58321 aggaataaaa acactgtcaa agatacaaca agttattcaa cttatcttat ctataaaatc
    58381 actcctccaa aagagtcggc caaaacgatt ttgaatcatc aaattggtgg ttatagtgat
    58441 ttttatggga aattgattga tggtaatttg aatggagctt atcttttcaa tgatgtagaa
    58501 cccagtgagt tgaaaaaaat tgccagtgaa ttagacacga cagaaacaac cttgaaaaat
    58561 tcaacgaatg actattggtg gaaaaaattg accgtctact atatgattga cgatgcaggg
    58621 gttggtgtga aaattttgac atcaagtaaa gatgccaatg acttgttaga gaaaaaaatt
    58681 gatgggttga ttgagaaatt taaacatgcc tatgcaaatg tgaaaattga aaaactggaa
    58741 accatcaact ctaaagaatt gaacgcaaat ggtgaaacag cgcttggctt aaatgtgaaa
    58801 aatctgattc ttcgtttagt tgttattgga gtggtttgtg tgattttggt tgtgatggga
    58861 aatgtgttag tttatctctt taatccaaca atcaatagag taggtgattt ttctcagtat
    58921 caaattgatt ttgtaacaga gattacaaca attgctaacc tagcagatgt tttgtcatac
    58981 aaaaatactg gacaggaatt gaccatcgtt agctcaaata aagctatcct agataaattg
    59041 aaacagagtc aagaagcttt aaaaggaatg cattttgtag atttacagga tgtatcatct
    59101 cttttggaaa gagatacagt ccttcttgtt gaagagtacg gagtgactcg ttataagaaa
    59161 tttgagcaaa gtcttcaaat tctcagaaac ttaaatcgtt ctatccttgg tgtagcaacc
    59221 tttaaattat aagtattctg atgtataagg tcttcaaaat cggctaagag ctgattttga
    59281 aggctttttc tatctgtctg cagaaatttt cttttggagt ctcctataga aagaccctag
    59341 ggaaaatcta tccgtataga ggagccttct tttgataaaa tcgtaaaaat ctagtataat
    59401 agatagaact gaaagtatga ggttactagc aatgaaaatg aaacaaatta gtgatacaac
    59461 tttaaaaatc acgatgtctt tagaggattt gatggatcgt ggaatggaga ttgctgactt
    59521 tctcgttcct caagaaaaaa cagaagagtt cttttatgct atcttggatg aactagagat
    59581 gcctgatagc tttctggata caggcatgtt gagcttccgt gtgactccaa aacctgataa
    59641 ggtagatgtc tttgtgacca agtcaaagat tgaccaaaat ctagattttg aagacttatc
    59701 ggatttgcca gatatggaag aattggctca aatgtctcca gatgaattta tcaaaaccct
    59761 ggaaaaaagc atcgcagaca aaaccaagga tgatatcgaa gccattcaat ctcttgagca
    59821 agttgaagcc aaggaagaag agcaagagca ggctgaacaa gaagctgaga gtaagaaaga
    59881 accttacatc tactacatcc tttcttttgc taagttggct gacttggtag tttttgccaa
    59941 gacagtgact tttgaaatgg aaacttctga actctacaaa atgaacgagc gctattattt
    60001 gaccatttta gtggatattg aaaatcatcc aagcccatat ccagcttggc tgttggcccg
    60061 tatgcgcgag tttgcagacg atagtgatat cagtcgctca gtcttacaag agtatggtca
    60121 agtcttgatg agtcacgatg cagtgctcaa tctgcaaaaa atcggctaat cctttctgga
    60181 aagctatttt cagattttaa gaagagcgaa tcgtctgatt cgttttttct ttttatactg
    60241 aaatagtgat ttactataat aggaattttc acaaaattct gttataatgg ctatattaga
    60301 aaatttcgag gagataaaga tgacagttaa aattgcttta ctaggatttg gtaccgttgc
    60361 aagtggtgtg cctttcctcc taaaggaaaa tggaggaaaa atcaatcaat cagcacattc
    60421 agatatcaaa gttgctaagg tattggtcaa ggatgaagat gaaaaaaatc gcttgcttgc
    60481 agcagggaat gactttaact ttgtaaccaa tgtggatgat attttatcag accaggatat
    60541 tactatcgta gtggaattga tggggcgtat tgagcctgct aaaaccttta tcactcgtgc
    60601 cttggaagct ggaaaacacg ttgttactgc taacaaggac cttttagctg tccatggcgc
    60661 agaattgcta gaaatcgctc aagctaacaa ggtagcactt tactacgaag cagcagttgc
    60721 tggtgggatt ccaattcttc gtactttagc aaattccttg gcttctgata aaattacgcg
    60781 cgtgcttgga gtagtcaacg gaacttccaa cttcatggtg accaagatgg tggaagaagg
    60841 ctggtcttac gatgatgctc ttgcggaagc acaacgtcta ggatttgcag aaagcgatcc
    60901 gacgaatgac gtagatggga ttgatgcagc ctacaagatg gttattttga gccaatttgc
    60961 ctttggcatg aagattgcct ttgatgatgt agcccacaag ggaatccgca atatcacacc
    61021 agaagacgta gctgtagctc aagagtttgg ttacgtagtg aaattggttg gttctattga
    61081 ggaaacttct tcaggtattg ctgcagaagt gactccaacc ttcctaccta aagcgcaccc
    61141 acttgctagt gtgaatggcg taatgaacgc tgtctttgta gaatctatcg gtattggtga
    61201 gtctatgtac tacggaccag gtgcgggtca aaaaccaact gcaacaagtg ttgtagctga
    61261 tattgtccgt atcgttcgtc gtttgaatga tggtaccatt ggcaaagact tcaacgaata
    61321 tagccgtgac ttggtcttgg caaatcctga agatgtcaaa gcaaactact atttctcaat
    61381 cttggctcca gactcaaaag gtcaggtctt gaagttggct gaaatcttca atgctcaaga
    61441 tatttccttt aagcaaatcc ttcaagatgg caaagagggt gacaaggcgc gtgtcgttat
    61501 catcacacac aagattaata aagcccagct tgaaaatgtc tcagctgaat tgaagaaggt
    61561 ttcagaattc gacctcttga ataccttcaa ggtgctagga gaataagatg aagattattg
    61621 tacctgcaac cagtgccaat atcgggccag gttttgactc ggtcggtgta gctgtaacca
    61681 agtatcttca aattgaggtc tgcgaagaac gagatgagtg gctgattgaa caccagattg
    61741 gcaaatggat tccacatgat gagcgtaatc tcttgctcaa aatcgctttg caaattgtac
    61801 cagacttgca accaagacgc ttgaaaatga ccagtgatgt ccctttggcg cgcggtttgg
    61861 gttcttccag ctcggttatc gttgctggga ttgaactagc caaccaactg ggtcaactca
    61921 acttatcaga ccatgaaaaa ttgcagttag cgaccaagat tgaagggcat cctgacaatg
    61981 tggctccagc catttatggt aatctcgtta ttgcaagttc tgttgaaggg caagtctctg
    62041 ctatcgtagc agactttcca gagtgtgatt ttctagctta cattccaaac tatgaattac
    62101 gtactcgcga cagccgtagt gtcttgccta aaaaattgtc ttataaggaa gctgttgctg
    62161 caagttctat cgccaatgta gcggttgctg ccttgttggc aggagacatg gtgaccgctg
    62221 ggcaagcaat cgagggagac ctcttccatg agcgttatcg tcaggacttg gtaagagaat
    62281 ttgcgatgat taagcaagtg accaaagaaa atggggccta tgcaacctac ctttctggtg
    62341 ctgggccgac agttatggtt ctggcttctc atgacaagat gccaacaatt aaggcagaat
    62401 tggaaaagca acctttcaaa ggaaaactgc atgacttgag agttgatacc caaggtgtcc
    62461 gtgtagaagc aaaataaaga atagaagata ggatggggaa actcttaacc agagggtttc
    62521 atatcctttt tgtgaaaaga agtttatact caatgaaaat caaagagcaa actaggaagc
    62581 tagccgcagg ctgctcaaaa cagtgttttg aggttgcaga tagaactgac gaagtcagct
    62641 caagacactg ttttgaggtt gcagatagaa ctgacgaagt cagtaacata tatacggtaa
    62701 ggtgacgcta acgtggtttg aagagatttt cgaagagtat tagttaaaaa cgtgataaag
    62761 gagaaataaa gatggcagaa atttatctag caggtggttg tttttggggc ctagaggaat
    62821 atttttcacg catttctgga gtgctagaaa ccagtgttgg ctacgctaat ggtcaagtcg
    62881 aaacgaccaa ttaccagttg atcaaggaaa cagaccatgc agaaacggtc caagtgattt
    62941 acgatgagaa ggaagtgtca ctcagagaga ttttacttta ttatttccga gttatcgatc
    63001 ctctatctat caatcaacaa gggaatgacc gtggccgcca atatcgaact gggatttatt
    63061 atcaggatga agcagatttg ccagctatct acacagtggt gcaggagcag gaacgcatgc
    63121 tgggtcgaaa gattgcagta gaagtggagc aattacgcca ctacattctg gctgaagact
    63181 accaccaaga ctatctcagg aagaatcctt caggttactg tcatatcgat gtgaccgatg
    63241 ctgataagcc attgattgat gcagcaaact atgaaaagcc tagtcaagag gtgttgaagg
    63301 ccagtctatc tgaagagtct tatcgtgtca cacaagaagc tgctacagag gctccattta
    63361 ccaatgccta tgaccaaacc tttgaagagg ggatttatgt agatattacg acaggtgagc
    63421 cactcttttt tgccaaggat aagtttgctt caggttgtgg ttggccaagt tttagccgtc
    63481 cgatttccaa agagttgatt cattattaca aggatctgag ccatggaatg gagcgaattg
    63541 aagttcgttc tcgttcaggc agtgctcact tgggtcatgt tttcacagat ggaccgcggg
    63601 agttaggcgg cctccgttac tgtatcaatt ctgcttcttt acgctttgtg gccaaggatg
    63661 agatggaaaa agcaggatat ggctatctat tgccttactt aaacaaataa aacagagagt
    63721 ggggcttccc actttcttca tttctagaat atgaatagaa gggatttatg aaacacctat
    63781 tatcttactt caaaccctac atcaaggaat caattttagc ccccttgttc aagctgttag
    63841 aagctgtttt tgagctcttg gttcccatgg tgattgctgg gattgttgac caatctttac
    63901 ctcagggaga tcaaggtcat ctctggatgc agattggcct gctccttatc tttgcagtaa
    63961 ttggcgtttt agtggccttg atagctcaat tttactcagc aaaggcagca gtaggttttg
    64021 ctaaggaatt gacaaacgat ctttatcgtc atattctttc cttgccaaag gacagcagag
    64081 accgtctgac aacttctagt ttggttactc gcttgacttc ggatacctac cagattcaga
    64141 ctggtatcaa tcaattcctg cgtctctttt tacgagcgcc cattatcgtt tttggtgcca
    64201 tttttatggc ttatcgaatc tcagctgagt tgactttctg gttcttagtc atggttgcca
    64261 ttttgaccat tgtcattgta gggttatctc gattggtcaa tcctctctac agtagtctca
    64321 gaaagaaaac ggaccaactg gttcaggaaa cgcgccagca attgcaaggg atgcgggtta
    64381 ttcgtgcttt tggtcaagaa aaacgagagt tacagatttt tcaaaccctt aaccaagttt
    64441 atgctagatt acaagaaaag acaggtttct ggtctagttt attaacacct ctgacctatc
    64501 tgattgtcaa tggaactctt ctcgttatta tctggcaagg ctatatttca attcaaggag
    64561 gagtgctcag tcaaggtgct ctcattgctc ttatcaatta cctcttacag attttggtgg
    64621 aattggtcaa gctagccatg ttgataaatt ctctcaacca gtcctatatc tcagccaagc
    64681 gaatcgagga ggtctttgcg gaatctcccg aaaacatcca ttcagaatta gaacaaaagc
    64741 aagttaccag tggtcgggtt ttacaagtcc aagaattgac ttttacctat cctgatgcgg
    64801 cccagccttc tctgagatac atttcctttg atatgactca aggacaaatt ctaggtatca
    64861 tcgggggaac gggttctggt aagtcaagct tggtgcaact cttacttgga ctttatccaa
    64921 tagataaggg gagcattgac ctttatcgag atggatctag tcctcgtaat ttagagcagt
    64981 ggcggtcttg gattgcttat gttccccaaa aggtcgaact ttttaaggga actattcgtt
    65041 ccaacttgac tttaggttta aatcaagaag tgactgatca agaactctgg caagccttgg
    65101 aaattgctca agctaaggat tttgtcagtg ataaggaagg actcttggat gctctagttg
    65161 aagcaggagg gcgaaatttc tcaggtggac aaaaacaaag gctgtctatc gcacgagcag
    65221 tcttgcgcca ggctccgttt ctcatcctag atgatgcgac ctcagcactg gacaccatta
    65281 ccgagtccaa gctcttgaaa gctattagag aaaattttcc aaacatgagc ttaattttga
    65341 tctctcaacg aacctcgact ttacagatgg ctgaccagat tctcctcttg gaaaaaggtg
    65401 agttgctagc tgttggcaag cacgatgact tgatgaaatc cagtcaagtc tatcgtgaaa
    65461 tcaatgcatc ccaacatgga aaggaggact agaatgaaac gacaaactgt aaaccagacg
    65521 ctcaaacgtt tagccgtaga tttagcaaac catcccttcc tccttttcct agcctttcta
    65581 ggaactattg cccaagttgg cttatcaatt tatctaccta ttctgattgg gcaggtcatt
    65641 gaccaagtcc tagtggctgg ttcatcacca gttttttggc agatttttct ccagatgctc
    65701 ttggtggtaa taggaaatac tctagtacaa tgggccaatc ctctcctcta taatcgtcta
    65761 atcttctctt ataccagaga tttacgagag cgaatcatcc ataaactcca tcggttaccg
    65821 attgcttttg cggatcggca aggcaatgga gagatggtca gtcgtgtaac cacggacatc
    65881 gaacagttgg cagctggctt gaccatgatt tttaaccaat ttttcattgg tgttttgatg
    65941 attttggtta gtattctagc catgctccaa attcatctcc tcatgactct cttagtcttg
    66001 ctgttgacgc cactgtccat ggtgatttca cgctttattg ccaagagatc ctatcatctc
    66061 ttccagaagc aaacagagac gaggggaatt cagactcagt tgattgaaga atcgcttagc
    66121 cagcagacta taatccagtc cttcaatgct caaacagaat ttatccaaag attgcgtgag
    66181 gctcatgaca actactcagg ctattctcag tcagccatct tttattcttc aacggtcaat
    66241 ccttcgactc gctttgtaaa tgcactcatt tatgcccttt tagctggagt aggagcttat
    66301 cgtatcatga tgggttcagc cttgaccgtc ggtcgtttag tgactttttt gaactatgtt
    66361 cagcaataca ccaagccctt taacgatatt tcttcagtgc tagctgagtt gcaaagtgct
    66421 ctggcttgcg tagagcgtat ctatggagtc ttagatagcc ctgaagtggc tgaaacaggt
    66481 aaggaagtct tgaccagtga ccaagttaag ggagctattt cctttaaaca tgtctctttt
    66541 ggctaccatc ctgaaaaaat tttgattaag gacttgtcta tcgatattcc agctggtagt
    66601 aagatagcca tcgttggtcc gacaggtgct ggaaaatcaa ctcttatcaa tctccttatg
    66661 cgtttttatc ccattagctc gggagatatc ttgctggatg ggcaatccat ttatgattat
    66721 acacgagcat cattgagaca gcagtttggt atggtgcttc aagaaacctg gctcacacaa
    66781 gggaccattc atgataatat tgcctttggc aatcctgaag ccagtcgaga gcaagtaatt
    66841 gctgctgcca aagcagctaa tgcagacttt ttcattcaac agttgccaca gggatacgat
    66901 accaagttgg aaaatgctgg agaatctctc tctgtcggcc aagctcagct cttgaccata
    66961 gcccgagtct ttctggctat tccaaagatt cttatcttag acgaggcaac ttcttccatt
    67021 gatacacgga cagaagtgct ggtacaggat acctttgcaa aactcatgaa gggccgcaca
    67081 agcttcatca ttgctcaccg tttgtcaacc attcaggatg cggatttaat tcttgtctta
    67141 gtagatggtg atattgttga atatggtaac catcaagaac tcatggatag aaagggtaag
    67201 tattaccaaa tgcaaaaagc tgcggctttt agttctgaat aagccattct cttttgaaag
    67261 tttatggacg aaaaaagttg ccttcgagtg acttttttgt tacaatagct agaaaaattg
    67321 ttcactgtaa tactcaatga aaatcaaaga gcaaactagg aagctagccg taggttgctc
    67381 aaagcacagc tttgaggttg tagataagac tgacgaagtc ag
//
LOCUS       DBEJAQ010000005        56726 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.005, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000005 DBEJAQ010000000
VERSION     DBEJAQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 56726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.005"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..645)
                     /gene="lytA"
                     /locus_tag="ACL297_000366"
     CDS             complement(<1..645)
                     /gene="lytA"
                     /locus_tag="ACL297_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGR0070690.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYM"
     gene            complement(1021..2391)
                     /locus_tag="ACL297_000367"
     CDS             complement(1021..2391)
                     /locus_tag="ACL297_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGR0070691.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(2698..3864)
                     /gene="recA"
                     /locus_tag="ACL297_000368"
     CDS             complement(2698..3864)
                     /gene="recA"
                     /locus_tag="ACL297_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGR0070692.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(3919..5175)
                     /locus_tag="ACL297_000369"
     CDS             complement(3919..5175)
                     /locus_tag="ACL297_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGR0070693.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(5260..6276)
                     /locus_tag="ACL297_000370"
     CDS             complement(5260..6276)
                     /locus_tag="ACL297_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGR0070694.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(6284..6802)
                     /locus_tag="ACL297_000371"
     CDS             complement(6284..6802)
                     /locus_tag="ACL297_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGR0070695.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGELFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(6792..7235)
                     /gene="tsaE"
                     /locus_tag="ACL297_000372"
     CDS             complement(6792..7235)
                     /gene="tsaE"
                     /locus_tag="ACL297_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGR0070696.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(7321..7941)
                     /locus_tag="ACL297_000373"
     CDS             complement(7321..7941)
                     /locus_tag="ACL297_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070697.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTVFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(8324..9187)
                     /locus_tag="ACL297_000374"
     CDS             complement(8324..9187)
                     /locus_tag="ACL297_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0070698.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            9379..9501
                     /locus_tag="ACL297_000375"
                     /pseudo
     CDS             9379..9501
                     /locus_tag="ACL297_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhrA family quorum-sensing system peptide"
     gene            9652..9876
                     /gene="pneA1"
                     /locus_tag="ACL297_000376"
     CDS             9652..9876
                     /gene="pneA1"
                     /locus_tag="ACL297_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HGR0070699.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            9883..10071
                     /gene="pneA2"
                     /locus_tag="ACL297_000377"
     CDS             9883..10071
                     /gene="pneA2"
                     /locus_tag="ACL297_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HGR0070700.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            10306..13176
                     /gene="lanM"
                     /locus_tag="ACL297_000378"
     CDS             10306..13176
                     /gene="lanM"
                     /locus_tag="ACL297_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="HGR0070701.1"
                     /translation="MSIISEKTFEEMLKLNNYNKKIFSNTVNYDYTEEQIRLYSEYAK
                     NSEWYKFAEGSFEIFRKHPQRINFKKDFSRILEPFIINLLKLINNYLENKEYEWIDKN
                     GNIFSSLVFYLEDLIYPWIVKPLVLEINSLREKGLLEGESEQQRYKYFITLFDKEENI
                     LNFYNKYPVLLRQISESCLRFYTYFIEILSNLENDFSVLEEELGLRGKLNDIKFGKGD
                     THSQGKTVLILFFDDAKIVYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDH
                     SYEEFIDYLPLEQKKKLPEYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFET
                     MLQQPLFDDKTGQSLLDTLFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNG
                     QVLINLNTDKVKFDIGKIDFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNK
                     TEEFKMLLKANLYGLKTRVLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKF
                     SNQGIVASECEQMRLLDIPIFYTDTNINEIYDDFGNHFISLHQFSGYDYFMKYLPNNN
                     LENLEKQLDIIEIYFPSFDQRKKGYKLNLSNRKKLPNESLVTILHNEVENIADSLVIN
                     NIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFMYYVKLLKDKSSYTQLYNK
                     MLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLACLNYIKSVLTELEKTDFA
                     SVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLGDTLYNDVSERDNSNFGYS
                     FGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIHSLKWCSGEFGTLVNHPQS
                     VPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELESLLCSVVMNKSKEGEYRIV
                     QTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLLEI"
     gene            13526..14230
                     /locus_tag="ACL297_000379"
     CDS             13526..14230
                     /locus_tag="ACL297_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGR0070702.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            14211..15140
                     /locus_tag="ACL297_000380"
     CDS             14211..15140
                     /locus_tag="ACL297_000380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HGR0070703.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSK
                     NNKYLEKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            15137..15601
                     /locus_tag="ACL297_000381"
     CDS             15137..15601
                     /locus_tag="ACL297_000381"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGR0070704.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYIL
                     DPAKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFLSIGIS"
     gene            15628..17244
                     /locus_tag="ACL297_000382"
     CDS             15628..17244
                     /locus_tag="ACL297_000382"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGR0070705.1"
                     /translation="MYVIQSLVPIANRYIIDTNFKDDSYSSRMLFTILFIFTVSFSLM
                     YLLRQIYVASLKYIMDKEISYDFMKHLIYLPYSFYEKRTLGDILFRANSIVYIREILS
                     NNFIAAILDLLMIVVYAVVLFSFSKYMVIFLISLSLALSIVMYPIIKISKNLIDKNIK
                     EKVNVQNITSEVISKNSDIKLTGEEEFWINKWDNFNTKQLIIGRKLDIHLSIVSSITN
                     VLQIILPVLTLIVGVNIKTFEQLTLGQIVAISTVSPYFISPIISLSDNYIQLMLLKGY
                     FLRIEDVFNTKSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGET
                     VAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLS
                     YGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMI
                     LLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKIL
                     VMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            17268..19007
                     /locus_tag="ACL297_000383"
     CDS             17268..19007
                     /locus_tag="ACL297_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGR0070706.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            19058..19825
                     /locus_tag="ACL297_000384"
     CDS             19058..19825
                     /locus_tag="ACL297_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGR0070707.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            19827..20459
                     /locus_tag="ACL297_000385"
     CDS             19827..20459
                     /locus_tag="ACL297_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070708.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(21388..21801)
                     /gene="ndk"
                     /locus_tag="ACL297_000386"
     CDS             complement(21388..21801)
                     /gene="ndk"
                     /locus_tag="ACL297_000386"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGR0070709.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(21912..25589)
                     /gene="rpoC"
                     /locus_tag="ACL297_000387"
     CDS             complement(21912..25589)
                     /gene="rpoC"
                     /locus_tag="ACL297_000387"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGR0070710.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(25622..29233)
                     /gene="rpoB"
                     /locus_tag="ACL297_000388"
     CDS             complement(25622..29233)
                     /gene="rpoB"
                     /locus_tag="ACL297_000388"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGR0070711.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            29649..29719
                     /locus_tag="ACL297_000389"
     tRNA            29649..29719
                     /locus_tag="ACL297_000389"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29681..29683,aa:Cys,seq:gca)
     gene            complement(29917..31248)
                     /locus_tag="ACL297_000390"
     CDS             complement(29917..31248)
                     /locus_tag="ACL297_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGR0070712.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(31477..32301)
                     /gene="endA"
                     /locus_tag="ACL297_000391"
     CDS             complement(31477..32301)
                     /gene="endA"
                     /locus_tag="ACL297_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HGR0070713.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(32340..32528)
                     /locus_tag="ACL297_000392"
     CDS             complement(32340..32528)
                     /locus_tag="ACL297_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGR0070714.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(32521..33804)
                     /gene="murA"
                     /locus_tag="ACL297_000393"
     CDS             complement(32521..33804)
                     /gene="murA"
                     /locus_tag="ACL297_000393"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGR0070715.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(33828..34058)
                     /locus_tag="ACL297_000394"
     CDS             complement(33828..34058)
                     /locus_tag="ACL297_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HGR0070716.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(34116..35153)
                     /locus_tag="ACL297_000395"
     CDS             complement(34116..35153)
                     /locus_tag="ACL297_000395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HGR0070717.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(35137..35625)
                     /gene="coaD"
                     /locus_tag="ACL297_000396"
     CDS             complement(35137..35625)
                     /gene="coaD"
                     /locus_tag="ACL297_000396"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGR0070718.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGRDIACYVPESILEEIRNEK
                     KD"
     gene            complement(35615..36154)
                     /gene="rsmD"
                     /locus_tag="ACL297_000397"
     CDS             complement(35615..36154)
                     /gene="rsmD"
                     /locus_tag="ACL297_000397"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGR0070719.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(36219..37211)
                     /gene="asnA"
                     /locus_tag="ACL297_000398"
     CDS             complement(36219..37211)
                     /gene="asnA"
                     /locus_tag="ACL297_000398"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGR0070720.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(37323..38006)
                     /locus_tag="ACL297_000399"
     CDS             complement(37323..38006)
                     /locus_tag="ACL297_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HGR0070721.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(38062..38802)
                     /locus_tag="ACL297_000400"
     CDS             complement(38062..38802)
                     /locus_tag="ACL297_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGR0070722.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            38836..39114
                     /locus_tag="ACL297_000401"
     CDS             38836..39114
                     /locus_tag="ACL297_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGR0070723.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            39192..40118
                     /gene="yidC"
                     /locus_tag="ACL297_000402"
     CDS             39192..40118
                     /gene="yidC"
                     /locus_tag="ACL297_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGR0070724.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(40193..40987)
                     /gene="pflA"
                     /locus_tag="ACL297_000403"
     CDS             complement(40193..40987)
                     /gene="pflA"
                     /locus_tag="ACL297_000403"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGR0070725.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(41113..42363)
                     /locus_tag="ACL297_000404"
     CDS             complement(41113..42363)
                     /locus_tag="ACL297_000404"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGR0070726.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(42430..43257)
                     /gene="purR"
                     /locus_tag="ACL297_000405"
     CDS             complement(42430..43257)
                     /gene="purR"
                     /locus_tag="ACL297_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGR0070727.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(43524..44465)
                     /locus_tag="ACL297_000406"
     CDS             complement(43524..44465)
                     /locus_tag="ACL297_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HGR0070728.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(44455..45711)
                     /gene="rmuC"
                     /locus_tag="ACL297_000407"
     CDS             complement(44455..45711)
                     /gene="rmuC"
                     /locus_tag="ACL297_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGR0070729.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(45713..46375)
                     /locus_tag="ACL297_000408"
     CDS             complement(45713..46375)
                     /locus_tag="ACL297_000408"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGR0070730.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(46338..46994)
                     /gene="rpe"
                     /locus_tag="ACL297_000409"
     CDS             complement(46338..46994)
                     /gene="rpe"
                     /locus_tag="ACL297_000409"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGR0070731.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(47005..47883)
                     /gene="rsgA"
                     /locus_tag="ACL297_000410"
     CDS             complement(47005..47883)
                     /gene="rsgA"
                     /locus_tag="ACL297_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGR0070732.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(47885..48757)
                     /gene="rsmA"
                     /locus_tag="ACL297_000411"
     CDS             complement(47885..48757)
                     /gene="rsmA"
                     /locus_tag="ACL297_000411"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGR0070733.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(49008..49301)
                     /locus_tag="ACL297_000412"
     CDS             complement(49008..49301)
                     /locus_tag="ACL297_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070734.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            complement(49294..49935)
                     /locus_tag="ACL297_000413"
     CDS             complement(49294..49935)
                     /locus_tag="ACL297_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070735.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(49937..51967)
                     /locus_tag="ACL297_000414"
     CDS             complement(49937..51967)
                     /locus_tag="ACL297_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGR0070736.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     LGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(52044..52340)
                     /locus_tag="ACL297_000415"
     CDS             complement(52044..52340)
                     /locus_tag="ACL297_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGR0070737.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(52660..53559)
                     /locus_tag="ACL297_000416"
     CDS             complement(52660..53559)
                     /locus_tag="ACL297_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0070738.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(53631..54191)
                     /gene="rnmV"
                     /locus_tag="ACL297_000417"
     CDS             complement(53631..54191)
                     /gene="rnmV"
                     /locus_tag="ACL297_000417"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGR0070739.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(54191..54964)
                     /locus_tag="ACL297_000418"
     CDS             complement(54191..54964)
                     /locus_tag="ACL297_000418"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGR0070740.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(55269..56162)
                     /locus_tag="ACL297_000419"
     CDS             complement(55269..56162)
                     /locus_tag="ACL297_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall surface anchor protein"
                     /protein_id="HGR0070741.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(56446..56580)
                     /gene="rpmH"
                     /locus_tag="ACL297_000420"
     CDS             complement(56446..56580)
                     /gene="rpmH"
                     /locus_tag="ACL297_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGR0070742.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT        17090 a        11889 c        10503 g        17244 t
ORIGIN      
        1 catatagcct gaactgtcaa agtagtacca agtgccattg attttctcaa acttgtcttt
       61 tggataagag ccgtctgaat gtacgtacca gtagccagtg tcattcttct gccagcctgt
      121 ttcaatcgtc aagccgttct caatatcata cttaaactgc tcacggctaa tgccccattt
      181 tgccaagtaa gggtatggat ccacatggtc tgagtggttg tttggttggt tattcgtgca
      241 atactcgtgc gttttaattc cagctaaact ccctgtatca agcgttttcg gcaaacctgc
      301 ttcatctgct agattgcgta agagttcgat ataaaggcgg tagtccgtca tgaactcttc
      361 tttagttgaa tggctttcaa tcagttcaac cgctgcatag gtttcagcat tccaaccgcc
      421 cccaacgtcc caggcaccat tatcaacagg tcctacctgc atgatgcaac cgttcccaac
      481 aatgtgcgag aaaaaaccta attctgggtc tttccgccag tgataatccg cttcattctg
      541 tacggttgaa tgcggattcc cagttgagtg tgcgtgtact tgcctatatg gttgcacgcc
      601 gacttgaggc aaatctgttc ttaatttact cacattaatt tccatattct actccttatc
      661 aattaaaaca actcattttt tacaatccag aaccagaaac tcctttattt ctaccttaca
      721 aagaagacaa tcttagtcac gattaggctt gtagatagaa cctcaaaacg cactattttg
      781 acactgtaaa taggactgac aaggtctgca ttctatctac aataacaccc cagactaaaa
      841 agcttttcaa aagtatattt ttacagtctc tatatgtcct tttcataaat actatacttt
      901 attatatcat ataaaagaag tcaaaagtct gttaaactat tttcaacacc aaactaaaga
      961 agagaacaca agttttttcg atgttcacta gaggaaatgg attttattca gtaaatccaa
     1021 ctaggattgc actttggttg ccaaaattgc ctttccttct tttatcaagg gatgacggaa
     1081 tagtgagaag tacagttgaa ctgtcatggc aacccagata agaggttcac aaaggataac
     1141 acccttatat cctgcccaag gaataatcaa aaccacaaaa acgatttttc cgattagttc
     1201 aataaagcta gaaactagag gaaggatctt ctgccccaag ccctgcaagc aattgcgata
     1261 aatcaacaag aggctcaaaa tgggataaaa ggctgaactg atttgcagat agagacttcc
     1321 attttctatc aagtaaccat ctgtcgaact agccaagaag gaaaccaagg ctggactggc
     1381 aaaaaagagg aaaatacaaa caaaaactgc ccaggatata cttaaacgac tgccgattcg
     1441 aagaccttga acaatgcggt caggtcgctt agctcctaga ttctgagaag caaaggtcgt
     1501 cattgatgca gaaatagcgg tcataggaag aagggcaaag gtcataatgc gtcgagctgc
     1561 cgtctgggca ctaataatca ctgcaccaaa tgtattaaca gaaaactgta aaatcacact
     1621 gccgatagat acaattgaac tcatcaagcc catagccaaa ccttgctcca agagatccgc
     1681 gtacaagctt ttgtcccatt tgaaatgttt aaactgtggc aagagttctg gcacactttt
     1741 acgaatataa taaaagcaga gaaccgctga taaaccttgc gaaataatgg tagcaagtcc
     1801 tgcggattga actcccagat gcaattgcgt aataaaatag agatccagaa ccacattaac
     1861 caaggcagag aaaatcagaa atcccagggc tgctagactg tcaccaatag accgcaacaa
     1921 gcctgcaaaa agattataag caaagctgac acctacacag gtcacaatca tagaaatata
     1981 ttgataagat tgaggaagaa tttctgcagg agtatctaag tattgcaaga gaggatacaa
     2041 gccaagaaag cccagcaaca taactagaat gctcaaaaga gcacctaaaa tccaggtggc
     2101 tgctactgct tccttgattt tagtgaaatt ccgagcccca taataacgag caatgacaat
     2161 ccccatgcca ttgccaacac caagtgtaaa gcctacaatc aggtcaaaaa tcgctgtcgt
     2221 cgctcctact gcagccaagg attcttgacc aagaaatcgt ccaacaatca agacatcagc
     2281 agtgttatag agctgttgaa aaatatttga tagcaagatt ggaaaggtga agcttaagag
     2341 cgagggaaga atcggaccat gtatcaggtc cactgttcgt tttttattca taaagctatt
     2401 atatcagctt tccagaggag cgacaagaaa cagcaaactt atatatagcg gaatgaaata
     2461 aggacagtca aatcgatttt taacaatgtt ttagaagtag atgtgtacta ttctagtttc
     2521 gatctattat agttggttga gtttggaata gtacgctgta gctgctaaaa catttctaga
     2581 aattaatttg actttcctaa taaagttgtt catatcttat ttcaagctac tatatataat
     2641 ctaaaaactt gaatcaaaaa tcgaaaaagt agcggatttc tccactgctt taacagctta
     2701 ttcttcaatt tcgatttcaa gttcatcgcc taagtcaagc ggaacttctt cattcactgc
     2761 ttcttctttc tttggctcat cttttttgtt ttcagtatct tgttctgaaa cttcttctcc
     2821 atcaatcaag ccaaatttag aacggacttg cttatcaatt tcatcaaaga tttctgggtg
     2881 ctctgccaag tatttcttag cattctcaga accttgccca attttttcat ctttgtaaga
     2941 ataccaagcc cctgcttttt tgataatatc caaatcgctt gcaatcttca aaagctcacc
     3001 agtcttagaa attccttctc cgtacataat ttcaactacg gcttccttaa acggtggagc
     3061 taccttattt tttacaacct taatcttagt ttctttaccg acattggttt ctttttgatc
     3121 accagttccc ttaatttgtg tattaccacg aacatccaag cggactgaag catagaattt
     3181 caaagcacgt ccgcccggtg ttgtttctgg atttccaaac atcactccaa ctttttcacg
     3241 caattggttg ataaaaatgg caattgtttt ggttttattg atagaggcgc caagtttacg
     3301 catggcctgg ctcatcatac gagcctgcaa accaacatgg ctatctccga tatctccatc
     3361 aatttccgca cgaggaacaa gggcagcaac tgagtcgact acgacaagat caactgcacc
     3421 tgagtcaatc aattttcccg caatctcaag accttgctct cctgagtctg gttgagacaa
     3481 gagcaattcg tcaatattga caccaagggc cgcagcataa gctggatcaa gggcatgttc
     3541 cgcatcgata aaggcagcaa tcccaccttc tttttgcgct tgtgcaactg catgaagggc
     3601 aaccgttgtc ttaccagatg actctgggcc atagatttcg atgatacgtc ccttaggata
     3661 accacctgag ccaagggcaa tgtcaagagc taaagaacct gagctcatca cttgcacctt
     3721 ttgctccgca cgttcaccca aacgcatgat tgatccttta ccaaagtctt tctcaatcaa
     3781 tttaagagca tcattcaagg ccttttcacg ttctgcccca aatttttttg aaatttcttc
     3841 taattttttt ggttttttcg ccattctatt ctcctacatt ctaatgatcc tcagacctat
     3901 ctactattat atcaaaagtt agtcacttaa taaagccttg cgaactaggt taaaggcatg
     3961 cataaccgca atatgacgta catctgctcg gcttctgcct ccaatattaa ccttgataac
     4021 ctcagttcct tgctcttgcg ccaagcctat gaagactgtc ccaactgggt gtccttctag
     4081 gctatctggt cctgccactc cggtcaaact aattccaaaa tcagactggg tcttgcttcg
     4141 tgcctgctca gccatcttct gagctgtaaa ttcagacacc acaccatgtt cttccaaatt
     4201 cttggcagga atatccaaca tccttgattt ttcctccaag ctataggtca caaaaccacc
     4261 cttaaatata cttgaagctc cagaaaaatt cgccacggta gcttggaaaa gacctgccgt
     4321 taaactctct gcagccgcga tggttttccc ttgccttttc agttcttcta ccacaatgct
     4381 ggctaaacta gtttcttccc cataaccata gcaaaagtct cgtaaagaaa ttccttcgaa
     4441 agtctggcag tccaagattt gattttccaa gatatccagc gcttgattcg cctcttcttg
     4501 actgctagcc tttgttgaca gacgtagagt gacttctcct gtcttggcat aaggggccaa
     4561 ggtaggatcg atctgattat caattaaatc agccaaaatc gtaaccaact ggctctcgcc
     4621 aatcccaaag aaacgaagaa ctcgggaata cagcttgctc cctgtcatca acttgggtag
     4681 aagttggttt aagaccatgg gtttcaattc acttggcgga cctggaagga cgacataggt
     4741 cactccgtct acttctaatt ttcctccaac agccagtcct gtttcgtttg gcagtggaat
     4801 cgctccttct acaatttgag cttgtctttc gttattcggt gttcgggcat agtctggtcg
     4861 cagggcaaaa aagatatcca acttctcctg agcctgagga tcaaagacta atgctttccc
     4921 taaaaattta gctagggttt gtttggttag gtcgtcctca gttggcccca aaccgcctgt
     4981 caaaatcacc agactgctac gttgactggc aatctcaagc aaagacaaga gacgaacttc
     5041 attgtctcct acagccgtct gaaaatatac atctacccca atctcagcta gtttttccga
     5101 caaaaactgg gcattggtgt tgacaatctg tcctgtcaaa atctctgttc caacagcaat
     5161 gatttctgct ttcatgtttc ctcctaccta tctattcgta tttttttgaa aaaatcgcag
     5221 gaattttcct acgattgatt tttttatttg tatcaaaagt taattatctt catcaccaac
     5281 aggtgctctg ccaaataaat cttcaaataa aaccgcattg gtttcaagct gagtaacttc
     5341 ttcttgtccc aaagaacgtc ggagtatatt ttgcatttcc aacatatgtg ctctcgaaac
     5401 aatctggtaa gaaacacctt gaagtatctc tccttcaccc tgcaactgct gagtttcaat
     5461 ggttttaaat gaatctttat agcctagcaa gttagggata ctttttgcag acaaatcaat
     5521 attggtctgc atattgtcac tcaaagcttt tagaatctct tgataatgac caacgctatt
     5581 taaactgaga gctttttcca tgactttttg aataacttca cgttgacgtt tttgacgacc
     5641 ataatccccc tcaggatctt ggtaacgcat tcgtgcatag actagggctt cttctccccc
     5701 aatatgttgc tccccaacac cgatagaaat agtattaaat tcttcttggt cactgataga
     5761 aattgggaaa cctaggatat tattgactgt aatacctcct actgcatcca ctagtttttg
     5821 caaccctctc atattgacca tcacatagcg atcaatatgg atattcatca ttttttgaat
     5881 ggtttctata gcaagctctg ctccaccatc tgcatatgct gagttcagtt tcgcttcatg
     5941 agcctgacca ttccctgatt caatgcgcgt cagaatatcc cgctctaaac tcatcattgt
     6001 tgtttttttc gttttaggat tcactgtcat caagatcatg ctatcacttc taccgaccca
     6061 agtttcagtt cgttcaacat ttccggtgtc cactcccatt aacagaatgg ttagaggttc
     6121 agtcgcttca ataaccttgg tttcttcacc gattttttta taggttttag ctaaggtttc
     6181 tgtcccttgt tgataaatag tataagcaaa aacacctact cctactacag ttacagaaag
     6241 taaagctagc accattccaa taattttttt aaccatattt ctactaacct atcagtttac
     6301 ccatcaagta aacatcgata aatttccctt cttctatata tgccccacgc tcttggctac
     6361 cttcaatgac aaagccatgc ttttgataaa gatggactgc tgcttgatta cgagtttgga
     6421 cagtcagttg gagacgacgc agaatgccac ttgcttgtgc ccactctatc gcttcttcta
     6481 gcaacaaact tcccaagcca ttattccaat atctttttcc aatcacaatg aagagttctc
     6541 caatatgacg gactctctta cgctggtcag ctgtaatatt tacaatacca gcaattttgc
     6601 catttaagaa tgcaagtaag gttatctgat tgtccgaact agcttgcttg ttgaggaata
     6661 tttccatctc ctcactagtc aagagaatac catctccgtc taggctggta aagtctgtct
     6721 ccaaactcac acgatttaaa aaggccacta attcagctgc atctttgggc tctgcttccc
     6781 taatgagcaa ttcatactcc atattgaagc tcctctaaca atttctcagc acgcaaaccc
     6841 tttgcctgaa aatttaaacg gcgtccatct gcttctttta gaatttccaa ttctaaataa
     6901 gcatctggca aggcatctcc taagagattg ccccactcaa taacagtcac gccgccacca
     6961 aagataaact catccaagtc gatagaatca gcatctcctt caatacgata aacatctagg
     7021 tgataaagtg gaagtcgacc ttcatactct ctcacgatag tataggtggg acttttaatc
     7081 atttgagaaa tctgtaatcc ttttgcaagt cctttagtaa aggtcgtttt acctgcaccc
     7141 agttctccag ttaagattaa aacatcattc tttgctaata gatggcccaa acgctcccct
     7201 aaggcttgca actcttcttc attttttgtg tacatactct tattatacca aaaacttttc
     7261 ttttgtgtct attttcctac taaacttatc atcataacat ccataaaaaa caggctttct
     7321 ctaaaagaaa atgagcgtaa caatgaccaa tacaagatct cggaaaatat gaccataaaa
     7381 ggaaacttcc ttcttaaccg aatttgggac aagataggct gcaaaaaaca agcccagtcc
     7441 aatataaatc agaagtgaga caatggtcat tggatttctt aagaaaagaa gtgttgctaa
     7501 aatagtcacc aacactgtct tttttctgtc tagcatagca agaaaatcgc gcacgtattt
     7561 tttcaagggt aaaaaaatca gcaaatctag cccaaatagg aaaaagaagg atggcaataa
     7621 aaagtcaact aattcttgct gcagcgtatt tttgatgaac aagttatctg acaaaacaag
     7681 aacagctcct aacaaattaa ttaagagtaa catactgtaa aaaagcttca cagacttctt
     7741 actggctagg acactatgga cttcttgctt acgggtataa agataattta ctccagcaca
     7801 gattcctgaa acgaaaacca tgcttccgat gaaaaaaact gtactttgtt taaaggacaa
     7861 gatgcattcc ttccatagga aacagctact caaactgatt tgaattaaag ctaacaaaaa
     7921 taagattctc attgatttca tcttctctct cccttcctac caatcattat actaggagaa
     7981 aagagagaac tgtttctaat cttctcaaat gtctctttaa gacgctaaac aaacactaga
     8041 gactaatact caatgaaaat caaagatcaa actaggtagc tagccacagg ttgctcaaaa
     8101 cactgttttg agattgcaga tagagctgac gtgatttgaa gagattttcg aagaatataa
     8161 atttgaaatc atgaaaatcc gtcaaacggg tggttgtttt gtctcgcacc tcacggagcg
     8221 agacggactc agagtcacat aattataagg ctgatagtat taatctaact atcagcctta
     8281 caggttattt aacgtttcag aaaaactata atgtcaagat taactaaaca gtatctaatt
     8341 ccttcaaata attttctatc ttcatcaaca ttaaaggatt gttataaatc ttacataact
     8401 ctcttgcttc tatataataa tttttgactt gttctctgtc tagaaatttg gctccagcat
     8461 ttcctacaag aataagtaga ggagccaatt ggtagcttgt ctgtctttgt ttacagagtt
     8521 caatcgtttc aagagcttct tggatggctt cattatattt ttcctttgat actaggtagt
     8581 gagcgtagtt gtaacgaact ctgatgtagc caaataaaaa ctcttgatgg tccaaatttt
     8641 ttgtctgata caactctatt aaatgagagt agtttgcctc atattcttgt tcacgaccca
     8701 ctaaggaata gaaattagat agagtattca acgcctttaa ataaatcaga gtatttgaag
     8761 agacttttaa taatatattt tccaatgacg aaattgcctc acacttactg tcatattgat
     8821 agaagtcaat tatagattta atccattcaa ggtaagttcg gtcttctaat gttagaaaag
     8881 tgcttcgttc tacctctatt ttataaagat attctaaatc gtcataattt ctgtcatcta
     8941 ataggcgagc agatagatgt ttgaaattag agaggttaga cttaacttcg atttgttcat
     9001 tgaaaaagta atccaaaggg acttcaagtc gttgagagag tttgaataac aagtctgcgg
     9061 agggaataaa atgacctctt tcaattttac taatctggct ttgttcacaa attccttccg
     9121 caagagtttg ttgggagagt ctcaactctt ttcttttcaa tctgaatttc tctgcgagtg
     9181 tattcattaa agataataaa ccttcctatt tgcttaattt cattataaag tttttagttc
     9241 aaaatagcaa gttaaaggaa taaataattc tttatggtaa taaacaaaaa aaaaagttaa
     9301 aaattacata aaaatacttg actaaataaa atatatctgt tagaataaaa acaaggaaaa
     9361 agaaaggggt ttcattgcat gagaaaaaaa cgtggaatta aaaaattagt tacatttgca
     9421 ttgctaggtg tttttatgtt tagtaataca attccttacc aacagtttat tcagaagaat
     9481 aaacaattgg agattcgagt gtaatcgcaa aagaagtcca atggtcttga tgttgggaag
     9541 gctgattaaa acccgatatt ttgcacgaga atgagagaat tataatttat ctgaaaaaac
     9601 gaaaaatata gagcaactta tttaactttt tataaggagg agttattact tatgaccaac
     9661 tttaattcaa acgaaaaatt ttgtggaaaa agtttaaaat cattgtctgc tgatgaaatg
     9721 agtttaattt atggtgcatc tgatggtgca gagccacgtt ggacgcctac tcctattatt
     9781 ttgaaatcag ctgcagctag ctcgaaagta tgcattagtg cagctgtttc tggtattggg
     9841 gggctagtta gctacaataa tgattgtttg gggtaaatta aaatgaaaaa tgattttgta
     9901 atcggaaaga gtttgaaaga attatctctt gaagaaatgc aattggttta tggtggcaca
     9961 gatggtgctg accctagatc aacaattata tgttctgcta cattaagttt tattgcatca
    10021 tatttggggt cagctcagac tcgttgtggc aaagacaaca agaaaaaata aaatattcaa
    10081 ttttaaaaat aaaagttgct ctttattttc ctctctcgta actaataatt ttgagagagg
    10141 ttttttttta aaacaatagt aagtcgtata ggtttgaggt aagtaaatga aatataaaat
    10201 taaaaatgtg cataaaacat tgtttattaa ggaaagatat caaatattat ctgaacgaaa
    10261 tgataattcc ttaaataact tttcagaaat aattggcgtt ctacaatgtc aattatttct
    10321 gaaaaaactt ttgaagaaat gttgaaatta aataattaca ataaaaaaat attttctaac
    10381 actgtgaact atgattatac cgaggaacag ataagactat attcagaata tgccaaaaat
    10441 tcggaatggt ataaatttgc ggagggttca tttgaaatat ttagaaaaca tccccaaaga
    10501 attaatttta agaaagattt ttctagaatt ttggaaccct ttattattaa tttgcttaaa
    10561 ttaatcaata attatctaga gaataaagaa tacgagtgga ttgacaagaa tggaaatatt
    10621 ttttcctctc tagtatttta tttagaagat ttaatctatc cttggattgt taaacctttg
    10681 gttttagaga taaattcatt gcgtgaaaaa ggtttacttg aaggggaatc ggagcagcaa
    10741 cggtacaaat attttataac attgtttgac aaggaagaga atatattaaa tttttataac
    10801 aaatatcccg ttttactgag gcaaatatcg gagtcttgtc ttcggttcta tacttatttt
    10861 atagaaattt tatcaaattt agaaaatgat tttagtgtgc tagaagaaga attagggcta
    10921 agggggaaat taaatgatat aaaatttgga aagggtgata cacacagcca aggaaaaact
    10981 gttttgatac tcttctttga tgacgcgaaa attgtttaca agcctaaaaa tttaataatc
    11041 aataactcac taaatactat tgctgagtat atccgaaagg ttgatgaaaa aattaggata
    11101 agaatacctc gaactattgc ttattcggat cacagctatg aagaatttat tgattatcta
    11161 cctctagagc aaaagaaaaa attacctgaa tattattata attttggtgt gcttttagca
    11221 tttatatatt tatttaatgg gagtgatata cattttgaaa atttaatttc ctatggagat
    11281 atgcctgtaa taatagactt tgaaacaatg ttacagcaac ctctatttga tgataaaact
    11341 ggtcagagtt tgctggatac cctgtttcac agggtgactc gaactttgct tcttccaaca
    11401 gagggagtaa aaagggagga tggtcttgat gttgagatga gtgctcttac aggaaatttt
    11461 aagaaggatg cttttaatgg tcaagtttta attaatttaa atactgataa ggtaaagttt
    11521 gatattggga aaattgattt tgaaggtggg aaaaacctgc ctgttcggga tggtgatata
    11581 gaatttgata agtatataaa agatttcaaa aaaggtttta gggattttta tctgatattt
    11641 gaagagttaa ataaaacaga agaatttaaa atgctattaa aagcaaactt gtatggttta
    11701 aagacaaggg tactttttag agacacaaat agctatgctt cagtactatc ttttttatat
    11761 catcctgatt tttatgaaga aatgttagat agagaaaaag cattggaaaa tctctggagc
    11821 aataagtttt ctaatcaagg tattgtagca agcgaatgtg aacaaatgag gttattagat
    11881 attccgattt tttatacaga tacaaatatc aatgaaatct acgatgattt tggaaatcat
    11941 tttatttccc tacatcaatt ttctggatat gactatttta tgaaatattt acctaataat
    12001 aatttggaaa atttagaaaa gcaacttgat atcattgaaa tttattttcc aagctttgat
    12061 cagcgaaaaa aaggatataa attaaactta tctaatagaa aaaaactccc aaatgaaagt
    12121 ttagttacta ttctgcataa tgaagtagaa aatattgcag actctttggt tattaataat
    12181 atttatgacg aatatccaat ttggagtata cctgttaatt ttaaaggttc taaatggtgt
    12241 cttgaacaaa cgggtaatga tatgtatagg ggtagagctg gaatattact gtttatgtac
    12301 tatgtaaaat tattgaaaga taaatcatca tatactcaac tgtacaataa aatgttatct
    12361 gcaattccgc catctgtttc tttatctaag accacatttg gtttaactgg ggatatagga
    12421 tatttcattg ttctatccat tatagaggat tacgatacaa atactctcgc atgcttaaat
    12481 tatataaaga gtgttttaac tgagcttgaa aagactgatt ttgcaagtgt ttcaaacaag
    12541 tctgactata ttaatggtct tctaccttta attaatacac tggtaagata ttataggaga
    12601 ggaatagaga aggaacgtgt tctaaggcta gtattaagtt taggtgatac gctatataat
    12661 gatgtaagcg agagagataa tagtaatttt ggatatagtt ttggccatgg actatcaggt
    12721 gtaattttta cattaaaaaa tatttataaa gtgacaagac ttaaaaaata taaagaatta
    12781 attttacaac tagttcctag ggagaaaatc aaaattcatt ctttgaagtg gtgtagtgga
    12841 gaatttggga cattagttaa tcatccccaa agtgttccag agggaattgt agctagttta
    12901 gataatgata cattttgcca tggaagtatg gccataataa atttttacat cgactacatg
    12961 aaagaaatgg atactagtat ggaattggag tctttattgt gtagtgttgt aatgaataaa
    13021 tcaaaagaag gagagtatcg tatcgtccaa acaagcaatt tcccagattt ttctttgtat
    13081 acaggtgtga ctggtattgc ttatactttt ttaaggtata tcgcactgaa tcagaatgac
    13141 aaaaagatag ttatcccctc attacttgaa atctagctta atttatagtt ttgtttctaa
    13201 aatagaggtg tccactcatt aagcaatata aaaataccaa gattgactag tttaatatgt
    13261 aattctataa aatattttta atagtgtagc ttttcgttga gataataata taataaagaa
    13321 aagttctatt ctaattgcta aacggcttga ggtatatgct actgttatag aaatgatttt
    13381 aaactaaaat caaaattact ccaaaaatag tattgatttt ggtttaatac aaaacatatg
    13441 taataatgtg agtcaacaaa agtctgtttt tgtaccatac tatcttgaaa aatctactaa
    13501 attcaaattg agaggatgaa ttaaaatgaa taaaatattt atatatgcag gagtgcgaaa
    13561 tcataactct aagactttag agtatactaa gagactttca agtatcattt ctagtagaaa
    13621 taatgttgat atatctttca ggacaccttt caattcagag ctagaaatat ctaattctga
    13681 ttcagaagag ttgtttaaga aaggaattga taggcaaagt aatgctgatg atggaggtgt
    13741 tataaaaaaa gagttattag agtctgatat tataattatc tcttctcctg tatatttaca
    13801 aaacgtatct gtggacacaa aaaactttat tgaaaggatt ggggggtgga gccatctttt
    13861 tcgattagct ggaaagtttg ttgtaacatt agatgttgct gagagtaacg gctctgataa
    13921 tgtgtcagaa tatcttagag atatattttc atatatggga ggacagatat tacaccaagt
    13981 ttcaattacc aattcactca aagatattgc agaggctcag ttgatggagg caacttataa
    14041 aattgaagat gtacttgaag ggaaaattaa gtacaaaaca acggattatc aagaaagagc
    14101 ttaccagaca ttaaaattaa ttttagaaaa ttatgattcc gaacattttg agaagatgta
    14161 ttgggagaaa aaacgccttt ttgaagctaa tagtttagag gaatggtatt atgttgaaaa
    14221 tattaaataa ctctgtcata tatttatgtt tgattccgtt attgttccta ttattgataa
    14281 tttttccaaa tgattcatta atctattatt ttcgattgat tttaatttcg ttaatatcaa
    14341 ttacattaca tgaactaggt cattttttag tgggaagatg tttatcttat aaactagaga
    14401 tgcttgctac tccctttttc ttctatttca gaaaaaaaat atatttcaaa tttccagtgt
    14461 tattagcatt cggttattgt caaatgagta atagaaacat tactaatgaa aaaaatagtg
    14521 ataggaattt ggtgttctac ttctttgggg gaggtggagc taacctaatt gtcgcaatat
    14581 tagcactttt gggatttgtg ccgtacgcat cagaattttt tattttgaat ataattcttt
    14641 ttctagttac ggtatgttta ccaattgatg gaacagatgg caatgctatt agagagatag
    14701 ttttgtattc aaaagattca aaaacatatc agagattctt tgcgaacagt ctctataata
    14761 atccttatat aacaatcgac gattttgcaa aattaacctc taaagaaaaa tctttttttt
    14821 caaaatttga aaaatgtttg ataaaccttt actttgaagt gaaaggtgaa gggtcagcat
    14881 ttacaattgc taatcatgaa gtttttgatt caaatattca ggaaaatatt atccatgagt
    14941 actataaatt actacataaa tcttctgatt ggattattcc acagaactta tctttagaag
    15001 aagttgtttt taccttgtca aactatattt actcgaaaaa taataaatat ttagaaaaga
    15061 ttaagtatct aaagaaatta gtagatttta gacaagaaga aattatcgac tttattttga
    15121 acaaggagga agtattgtga aaattccaat gatatatcaa atggaaaatt cagaatgtgg
    15181 attagcatgc tgtgccatga tattgaacta ttttaaatat gagatttctt taaatgaact
    15241 acgtgaaatc tacccatcat ccagatctgg atattctctc ctatctataa gtaaagtttt
    15301 aggagatttt aatataagtt ctcatgcttt taaagcttcg gtaagagatt taaaaccgct
    15361 cagtttccca ctcatttgct tctgggagag ttctcatttt attattcttg aaaaaattag
    15421 taaaaacaag ttttatattt tagatcctgc aaaaggcagg cagagaatgt caataagtga
    15481 atttgaaagg cattattcaa atatcatttt aacatttaaa aagttagata gctttatgtc
    15541 tcgtaaagat aataagaagt cgcctgtttt aaagtatttt ttaagtatag gaataagcta
    15601 gggattttat tttttgtaac agcattattg tatgtaatac aatcattagt acctatagct
    15661 aatagataca taattgacac gaatttcaag gacgattcgt attcgtctag aatgttattt
    15721 actatattat ttatatttac tgtttcattc tcactaatgt atttattaag acagatatat
    15781 gttgcatcct taaaatatat aatggataaa gagattagct atgattttat gaaacatttg
    15841 atatatttac cttacagttt ttatgaaaaa cgtactttag gggatatact ttttagagct
    15901 aactctattg tttatataag agaaatacta tcaaataatt ttatagcagc tatacttgat
    15961 ttgttaatga ttgtggttta tgctgtggtt ttatttagct tttctaagta catggtaatc
    16021 tttttaatat cactaagtct agctctatct attgtaatgt atccaatcat aaaaatctca
    16081 aaaaatttaa ttgataaaaa tataaaagaa aaggttaatg ttcaaaatat tacttccgaa
    16141 gtaatttcta aaaatagtga tattaagcta actggagaag aggaattttg gattaacaaa
    16201 tgggataatt ttaatacaaa acagctcatc ataggtcgaa aacttgatat acatttatca
    16261 attgttagta gtataacgaa tgttttacaa attattctcc ctgttttgac ccttattgta
    16321 ggtgtaaata taaaaacatt cgaacaattg acgttaggac aaattgtagc aataagtaca
    16381 gtctcaccat actttatttc tcctataatt tctttaagtg ataactatat acaattaatg
    16441 ttattaaagg gatatttttt aagaatagag gatgtgttta atactaaatc cgaattaatt
    16501 ccagaaagag tcagtcaaga tataaaattt gataaaaaaa tagaattaaa agatatttgg
    16561 tataaatatg gattatttga tgattatgtt ttgaaaggaa taaatgttac tattaaaaaa
    16621 ggagaaactg ttgctattgt tggagaatca ggttcaggta agagtacatt agctaaaatt
    16681 ttattaggtt tattagaacc taatattggt tcaatagaag ttgatggagt agaaaaagaa
    16741 gaaattggtc aaacattgta tagaaagatt tttggagcag tgttacaaaa ttcaacccta
    16801 agttatggta ccttaagaga gaatttgaca tttggacact ttgtttcaga tgaagaatta
    16861 atgacaaatc taaattcaat tggtcttagc aatgtagtta aatctttacc tcttggatta
    16921 gagacaatca tcgctgaaga aggtaataac ttttctggag ggcagcagca aatgatactt
    16981 ttagctcgtt gtcttttgtc gaaaccttcg gtagttgttt tggacgaagc aacaagtagt
    17041 ttagataatt tatctcaaca aattacaact tcttacttaa gtgaaatcgg taccactaag
    17101 attttaattg cccatcgact agatactatc aagtctgcag ataagatctt agtaatgcat
    17161 aatggtgaaa ttgtagagat tgggacccat agagaacttc ttgaactagg aggcatttat
    17221 aagcaattgt attcaaataa ttagtttttg attaaaaggg taaatttatg aagattatga
    17281 aaaaaaaata ttggacttta gcgatattat tcttttgttt gttcaataat tctgttactg
    17341 ctcaagaaat acctaaaaat cttgatggca atataactca cactcagact agcgaaagtt
    17401 tttctgaatc tgatgaaaaa caggttgact attctaataa aaatcaagaa gaagtagacc
    17461 aaaataaatt tcgtattcaa atcgataaga cagaattatt tgtaacaaca gataaacatt
    17521 tagaaaaaaa ctgttgtaaa ttggaacttg aaccacaaat aaataacgat attgttaact
    17581 ctgaaagtaa taatttacta ggcgaagata atttagataa taaaattaag gaaaatgttt
    17641 ctcatctaga taatagagga ggaaatatag agcatgacaa agataactta gaatcgtcga
    17701 ttgtaagaaa atatgaatgg gatatagata aagttactgg tggaggcgaa agttataaat
    17761 tatattctaa aagtaattct aaagtttcaa ttgctatttt agattcagga gtcgatttac
    17821 aaaatactgg attactgaaa aatctttcaa atcactcaaa aaactatgtc cccaataaag
    17881 gatatttagg aaaagaggag ggagaggaag gaataatatc agatattcaa gatagattag
    17941 gtcatggtac ggctgttgta gctcaaattg taggggatga caatattaat ggagtaaatc
    18001 ctcacgttaa tattaacgtc tatagaatat ttggtaagtc gtcagctagt ccagattgga
    18061 ttgtaaaagc aatttttgat gctgtagatg atggcaatga tattatcaat cttagtactg
    18121 gacaatattt aatgattgat ggagaatatg aggacggaac aaatgatttt gaaacatttt
    18181 tgaagtataa aaaggctatt gattacgcga atcaaaaagg agtaattata gtagctgcat
    18241 tagggaatga ctccctaaat gtatcaaatc agtcagattt attgaaactt attagttcac
    18301 gcaaaaaagt aagaaaacca ggattagtag ttgatgttcc aagttatttc tcatctacaa
    18361 tttcggtcgg aggcatagat cgcttaggta atttatcaga ttttagcaat aaaggggatt
    18421 ctgatgcaat atatgcgcct gcaggctcaa cattatctct ttcagaatta ggacttaata
    18481 actttattaa tgcagaaaaa tataaagaag attggatttt ttcggcaaca ctaggaggat
    18541 atacgtatct ttatggaaac tcatttgctg ctcctaaagt ttctggtgcg attgcaatga
    18601 ttattgataa atacaaatta aaagatcagc cctataatta tatgtttgta aaaaaaattc
    18661 tggaagaaac attaccagta aaaaatggta taaaagtgtt aaatatacca aacgtattga
    18721 gatatgattt gaatatgtta caattagaat ataaaaatga acaaagttgg gatagtttca
    18781 tagataatgt taatttaatt gagttggaag agagaattca aactactatt ggaattaaac
    18841 aaataaacac acacaatatt attactattg cccgagaagg gtactctcaa aattatttac
    18901 ctaacacttc agaaaataca tataattcat tacaagtcag tttagttgga gtattactac
    18961 tttttataag tatggtaaat attttatggg ctaaaaaaag taaatgaaaa taaaatttgg
    19021 agccctctga aaaagtaagt cctacagttc aactaaaatg agtcaaaaga tgaatcacct
    19081 tgatgtaggg gagtttgtct tattgctgcc tgaacacctc cgttcagagg aagaacatta
    19141 taaatctgtt tttgaagacg acttaaccag tcgcatatct agtcaagatg aacgacagca
    19201 aatgactgct acggtaggtt atttagaatc aggtcaggat cgttttgtgt ataatacgac
    19261 ccctatttct taccagcagt ttttgaaaga tccaatcatc attgttataa caccccaatc
    19321 aactggtcca cagtccattt tgttttggat agacgcagta cagaactacg ttctctttaa
    19381 tcaattgtct gatgcccagg agcttatcca gagacaaggc attgaaaatt gggtctcaga
    19441 aatgcaaaca ggttaccaca actacatcac attattggat aatatccaga gggaacgttg
    19501 ggtaatgcta gcaggagctg tgcttgggat tgcaacttca atcttgttgt ttaacactat
    19561 gaataggctc tactttgaag aatttagacg tgccattttt atcaaacgca ttgcaggtct
    19621 caggttctta gaaatccatc gcacttatct ctttgctcaa ctgggtgtgt ttttactggg
    19681 atttgttgcg agtgtatttc ttcaggtaga gataggagtt gctttcttag tcttgttact
    19741 ctttactggt ctatctcttt tacagttaca tgtccaaatg cagaaagaaa acaagatgtc
    19801 catgcttgtt ttgaagggag gttaatatga ttgaacttaa acaggtgagt aaatcttttg
    19861 gagaacgaga gttattttcg aatctttcaa tgacatttga ggctggaaaa gtctatgcct
    19921 taattggttc aagtggtagc ggaaaaacaa ccttgatgaa catgattggg aaattagaac
    19981 cttatgatgg gacgattttt taccgaggta aagacttggc caattataaa tcaagtgatt
    20041 ttttccgtca cgaattgggc tacctcttcc agaactttgg cttaattgaa aaccaaagta
    20101 ttgaagaaaa ccttaagcta ggtctcattg gtcaaaagtt gagtcggtcg gaacagcggt
    20161 tgaggcagaa gcaggcttta gaacaggtcg gcctggttta tcttgaccta gataagcgca
    20221 tctttgagtt atcgggcgga gaatcgcaac gggttgcctt ggcaaaaatt atcttaaaga
    20281 atccaccctt tattctggca gatgagccaa cagcttcaat agacccagca acctctcagt
    20341 tgattatgga gattttgcta tctcttcgag atgataatag gctaatcatt atcgcaacac
    20401 ataatccggc aatttgggag atggctgatg aagtgttcac gatggatcat ctgaaataaa
    20461 aatccttgtt tttaattgca cgatgagtta ctgaaatatt atcatgaatc aagaattgga
    20521 gttaatttag aattgtactt tattaatatt gaggtaactt tttcttgata aaggaagaaa
    20581 taatggagag gaagttagaa tgaaaaaatt cgacaattat attattgaga agccttgcga
    20641 ttctaattca gataaactgc aaaaaatctt aataattgaa agtttggtag atgatatttt
    20701 gcaattttct ctcagaatca ataatagtgt aggagagatt ttcctcctac aaccgtttta
    20761 aaagaaaact atctttattc catgttattt tgaggaagat attgtgaaag tcaaagatga
    20821 tgataaagtt gagtggaatt tgttagaatt tcaaaaattt agagcatttt tggcttagta
    20881 atctgtgttg aaggctcaaa acctatggta aaaaagtagc tttgaaaacg tattgcctcc
    20941 aaagatttag ttaaataatg atttaacaca aaaagaaatt attgaagttc tggaaagatg
    21001 ttgtttcagt attgagaaaa ggtgggaaaa acttgcgatt ttcacagaga aaggaagaaa
    21061 aagtatagaa atatagtcaa ttgaaacaag aacaggataa aagaaccttt tgtgccatat
    21121 ttttctcctt tcgctttaca attggattga acacctttat tgtatcgcgt ttggagtttt
    21181 tttggtataa ccttcgacgc acacccgcat agcgggtgtt ttttttgtct cgcacctaac
    21241 ggagcgagac aaactaatag tcacttaatc aaaaaacgca ccatatcaaa aactaaaaag
    21301 tttgatatca tgcgtcatgt cttaaactaa ttgactatac tttctattca aatgagcttt
    21361 taaccaattg attgagccaa tccactctta aaaccaaaga gcaatttctc gcttagctga
    21421 ctcttctgaa tctgaaccat gtacaacatt ttggataatc tcattttctc cagcagcttt
    21481 tgcaaaatca cctcgaatag tgcctggtaa agcttcttct ggacgagttg cacccatcat
    21541 ggtccgccaa gtttcgatta ctttgggacc agaaatgaca cccacaagaa ctggacctga
    21601 agtcatgaat tcacgaatcg gtgggtaaaa actctgacca accaagtcct gatagtgctg
    21661 gtcaatcaac tcttctgaaa cctgtgaacg aaactccaat ttttcgattg taaatccacg
    21721 ttgttcgatg cgctttaaca cttcacccac tagccctctt tttacaccat ctggtttgat
    21781 gataaagaat gtttgttcca tacccgtctc ctttgtcagc ttctttcttt tattttacca
    21841 catttcgtgg aaaaatggag aaagttttca gaagagagaa tgagagaacc ctcgggttct
    21901 ctcattctct cttattctac tgtttcttcc acagtttcaa cggcagtatc cacaactact
    21961 tctgttgttt cttcatttcc ttcttcctct actggaggat taaggtattc ttcttcgttg
    22021 acagcatgtg gttcaaggtt acggtaacgg gccataccag taccagctgg gatgatctta
    22081 ccgatgataa cattttcttt aagtccaagg agatggtctt tcttaccacg gatagctgcg
    22141 tcagtaagga cacgagttgt ttcctggaag gaagccgctg acaagaaact gtttgtttca
    22201 agtgaggctt tggtaattcc cataaggact gggcgacctg tcgctggaac tccacctgcg
    22261 ataaggacat ctttgttggc atctgtaaag tcattgatat ccatgagggt acccatgaga
    22321 agatctgtat cacctggatc catgacacgg actttacgga tcatttgacg aaccattacc
    22381 tcgatgtgtt tgtcaccgat ttctacccct tggctacggt aaactttttg tacttcaccg
    22441 agaaggtacg tttcaactga caagacatca cgaactgcaa ggagacgttt tggttggata
    22501 gaaccttctg tcagagcagc accacgcgct acttggcccc caacttcgac acgcatacga
    22561 gctgtaaatg gaacgacata ttcaccttcg ccagtttcac ccttaacaaa gactttcttg
    22621 gtacgagttg atgcatcttc ttcgatagca gtaacttgtc ctttaacctc tgtaataacc
    22681 gcttcccctt taggattgcg ggcttcaaag atttcttgga cacgaggaag accctgagtg
    22741 atatcggtat ttgaggcaac cccacctgtg tggaaggtac gcattgtaag ctgtgtacca
    22801 ggttccccga tagattgggc agcgattgta ccaactgctt caccaacttc aaccgcatca
    22861 ccagtcgcca agttgatacc gtaacagtga cggcagacac cgtgacgagt gttacatgta
    22921 aatacagaac ggatagtcac ttcttccaca ccagcattga caatttcacg cgccttgtct
    22981 tctgtaatca attcatttgg accaataatc actgcaccag tttctggatg tttaacagtt
    23041 ttcttagtgt aacgaccgtt gagacgctct tcgagagact cgatcatctc ttttccttct
    23101 gcgatagaac ggatcaagag accacggtca gttccacagt cgtcctcacg gatgataacg
    23161 tcttgggcaa cgtcgaccaa acgacgagtc aagtaacctg agtcggctgt cttaagggcc
    23221 gtatcggtca tacctttacg agcaccgtga gttgagaaga acatttccaa taccgacaaa
    23281 ccttcgcgga agtttgaaag gattggcaat tccatgatac gtccattcgg agcagccatc
    23341 agaccacgca taccggcaag ctgtgagaag tttgagatgt taccacgggc tccagagtcc
    23401 atcatcataa cgattgggtt cttaggatct tgattagcaa tcaagcgttt ctcaagtttt
    23461 tcacgggcag cacgccattc agctgtaaca gcattgtaac gctcgtcgtc tgtgatcata
    23521 ccacgacgga attgtttggt gatttgttcg acacgtttgt gtgattcttc aatgatttca
    23581 gccttgtcat caacgactgg gatatcggca atacccactg tcaatcctgc aagagttgag
    23641 tggtggtaac cgaggttctt catgcggtca agtagggcag aagtttctgt cgtacggaaa
    23701 cgtttgaaga tttcagcgat gatatttccg aggtttttct tcttgaatgg agggttgagc
    23761 tcaagattgc tgatagcttc cttgatagct ccaccaagtg gcaagaagta tttagctgga
    23821 acaccttctg tcaagttggc attgtttggt tcttgcaagt atggtagccc ctctggcatg
    23881 atatcgttga agagaatttt accaactgtt gtaagcaaga ccttatgtct ttgctcttct
    23941 gtccaaggct tgttgaggct gtctgttgcg ataccaacac gtgagtggag gtgaacataa
    24001 ccattgcggt aagccataac cgcttcgtca cggtctttga agaccattcc ttcaccttcg
    24061 cgaccagctt cttccatggt caagtagtag ttacccaaaa ccatgtcctg agatggagta
    24121 actaccggtt tcccatcttt cgggttcaag atgtgctcag cagctagcat gaggatacga
    24181 gcttctgctt gtgcttcttc tgaaagtggt acgtggatgg ccatttggtc cccgtcaaag
    24241 tcagcattgt aggcttcaca gacaagtggg tgcaagcgaa gagccttacc atcaatcaag
    24301 actggctcga aggcttggat acccaaacgg tgaagggtcg gtgcgcggtt caaaagcact
    24361 gggtgttctt taatcacttc ttcaaggata tcccagatac gctcatctcc gcgttccacc
    24421 aagcgtttag ctgctttgac gttttgcacg atatcacggg caacgatttc acgcatgaca
    24481 aatggtttaa agagttcaat cgccatttca cgtggcacac cacattggta catcttaaga
    24541 gttggaccaa cggcgataac ggaacgtcct gagaagtcaa cacgtttacc gagcaagttt
    24601 tgacggaagc gtccttgttt acctttaagc atgtggctca atgatttcaa tggacggcta
    24661 cctggtcctg tgattggacg accacgacga ccattgtcaa tcaaagcgtc aactgcttct
    24721 tgaagcatac gcttctcatt ttgaacgatg atacctggtg catttaactc aagcaaacga
    24781 gccaaacggt tgttacggtt gataacacgg cggtaaaggt cattcaagtc agatgaggca
    24841 aaacggccac catccaactg caacattgga cgaagatctg gtgggataac cggaaggatg
    24901 ttaagaatca tccattcagg tttgtttcca gacttgtaaa aggcatccaa aacatccaaa
    24961 cgacggatgg ctttgacacg tttttgtcca gtagcagttt tcaattcttc tttgagttca
    25021 gcaatttctt tttcaagatc tacttgcttc aaaaggtctt ggatggcttc cgcacccatc
    25081 ttagcaacaa atgaaccata accatattcg cgcaagcgct ctcggtattc gcgctctgtc
    25141 atgatagact tgtgctcaag tggtgtatcc ttaggatcaa tcaccacata agccgcaaag
    25201 tagataactt cctcgagggc acgagggctc atatcaaggg tcaagcccat acggcttgga
    25261 atccccttga agtaccagat gtgagataca ggagctttca attcgatatg tcccatacgc
    25321 tcacgacgaa ctttcgtacg cgttacttca accccacagc ggtcacaaac aattcctctg
    25381 taacgaatgc gtttgtactt accacaagca cattcccagt cttttgtagg accaaagatc
    25441 acttcatcaa agagtccttc acgttctggt ttcaaggtac gataattgat tgtttcaggt
    25501 tttttgactt ctccataaga ccatgaacgg actttacttg gagaagctag ggtgatttgc
    25561 atacttttaa aacgatttac atcaaccact atttcttccc tttctattct aagtgaactg
    25621 cttattcttg ttcagcagct tcttctgttg cttccgcttt tgttgctttc tcagcttctt
    25681 cagcttcaaa ggctgcttta gcctcttggg ctgctttttc gcgggctttt tcaaggtcat
    25741 ctacgtggat gacatcttcg tccattcctt catccaagtc gcgaagttcc acttcttggt
    25801 catcttcgtc taggacacgc atgtcaagac caagagattg caattctttg acaagaactc
    25861 ggaaggattc tggaacacct ggttttggaa ttggtttgcc ttttgtaata gcttcatagg
    25921 ctttcaaacg tccgttgata tcgtccgact tgtaagtcaa gatttcttga aggacatttg
    25981 acgcaccgta ggcttcaaga gcccaaacct ccatctcacc gaaacgttgt ccaccaaact
    26041 gagccttacc tccgagtggt tgttgggtaa cagttgagta tggtccgact gaacgcgcgt
    26101 gcaatttatc atcaaccatg tggtggagtt tgatcatgta catgactccg acagaaacac
    26161 ggttatcaaa cggttcacca gtacgtccat cgtaaaggat cgttttggca tcgctatcca
    26221 tacctgcttc tttaacagtt gaccaaagat cttcagaact tgctccatca aagactggtg
    26281 tagcgatgtg aataccaaga gtacgagctg ccataccaag gtgaagctcc ataacctgac
    26341 cgatattcat acgtgatggt accccaagtg ggttcaacat gatgtcgact ggagttccgt
    26401 ctggaaggta aggcatgtct tctacaggaa cgatacgaga gacaacccct ttgtttccgt
    26461 gacgtccggc cattttatct ccgaccttaa tcttacgttt ttgagcgatg taaacacgaa
    26521 ccaacatgtt aacacctgat tgcaactcat ctccatttac acgtgtaaag atcttaacat
    26581 cacgaacgac accatcggca ccgtgtggta cacgaagaga agtatcacgc acttcacgag
    26641 acttgtctcc aaagatagcg tgcaagagac gttcttcagc tgaaagatct ttctcaccct
    26701 taggtgttac tttacctaca agaatatcac cttctttaac ctcagcacca atacggataa
    26761 tccccatttc gtcaaggtct ttgagggcat cttcaccaac gtttggaatt tcgcgagtga
    26821 tttcttcagg cccaagcttt gtatcgcgcg tttctgattc gtattcttca aggtgaacag
    26881 atgtgtagac atcgtccttc accaagcgtt cgctcatgat aacggcatcc tcgaagttgt
    26941 aaccttccca agtcatgtag gcaacgattg ggttttgtcc aagcgccatt tctccatttt
    27001 ccatagaagg tccgtcagcg atgaaatcgc ctttttcaac gacatcacca acttttacga
    27061 gagtgcgttg gttgtaagca gtacctgagt ttgaacgacg gaatttttgg atgtggtaaa
    27121 catccaatga accatcttca cgacgaactt ctaccttgtc agcatctgcg taagtaactt
    27181 taccatcata ctgagcaatc acagccgcac cagaatcgtg ggctgcttgg tattccatac
    27241 cagtaccaac gtaaggtgcc tgaggattaa tcaatggcac agcctgacgt tgcatattgg
    27301 ctcccatgag ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa
    27361 cggcaactac ctgttttggt gaaacgtcca tgtagtcaac aatattagct ggatactctt
    27421 ggttgacccc ttggtgacgt cccatgacaa tcttctcagc aaaggttcca tcttcattca
    27481 gacgagagtt agcctgagct acagtatatt catcttcttc atcagctgtc aaccaaacaa
    27541 tttcgttcgt gacaacacct gtttcacggt caaccttacg gtatggtgtt tgaacaaaac
    27601 catatttgtt caagtgtccg taagatgaca agttattgat caaaccgatg ttaggtcctt
    27661 caggtgtctc gattggacac atacgaccat agtgagtgta gtgcacgtca cgcacttcat
    27721 atccagcacg gtcacgagtc aaaccaccag gtcctaaggc tgacaaacgg cgtttgtgag
    27781 acaactcaga aagcgggttg tgttggtcca tgaactgtga caactgtgat gaaccaaaga
    27841 attctttaac tgcagctgtt acaggacgaa tattgataat ttgttgtggt gtcaagactt
    27901 cattgtcctg aacagacata cgttcacgga cattacgttc catacgagaa agtcccaaac
    27961 gtacttggtt ggcaagcaat tcaccaaccg cacggatacg acgatttcca aggtggtcga
    28021 tatcatctac acggccaagt ccttcagcca agttgaggaa gtagctcatc tcagcaagga
    28081 tatctgcagg agtcaccgta cgaaccttgt catctgggtt agcattacca atgatcgtta
    28141 cgacgcgatc tggatcagtt ggagcaataa ccttgaattt ttgaagaaca acaggctcag
    28201 tcacaacggc tgcatcgttt gggatgtaga caatcttgtt caagtcgcca tccaaatggc
    28261 tttcaatgct ttcaatcacg ctacgagtca taatcgtacc agcttctacc aagatttctc
    28321 cagtttcagg gtctaccaat ggctctgcaa tggtttggtt gagtaaacgt gttttaacat
    28381 tgagtttttt attgattttg taacgaccaa ctgctgccaa gtcataacga cgtgggtcaa
    28441 agaagcgagc tacaagcaag ctacgtgagc tttcagccgt cttaggctca cctggacgaa
    28501 ggcgttcgta aatttctttc aaggcttcgt ctgtacgaga gtccattgga ttcttgtgga
    28561 tatctttttc aacagtgttg cgaaccaatt cgctgtcacc aaagatatca aagatttcat
    28621 catcacctga gaaaccaaga gcacgaacca aggttgtaaa tggaatctta cgagtacggt
    28681 cgatacgagt gtaggtgata tcttttgagt cgctttcaag ttccaaccaa gctccacggt
    28741 tagggataac agttgaacca tagcccacct taccattttt gtctactttg tcgttaaagt
    28801 aaacacctgg tgagcggacc aactgagaaa cgataatacg ttcaccacca ttgatgatga
    28861 aagtacccat ttctgtcatg attgggaaat caccaaagaa aacttcttgg gtcttgattt
    28921 cgcttgtttc tttattgatc aaacggaagg ttacaaaaat tggtgctgag tagctagcat
    28981 cgtggatacg agcttcttct agcgtatatt ttggttcctt gatttcatat ccaacaaatt
    29041 ccaactccat tgtgtctgtg aagtttgaaa ttggcaatac atcttcaaac acttccttaa
    29101 gaccgtgatc taggaaagct ttgaatgagt cagtttgaat ttcaatcaaa tttggtaagt
    29161 caagaacttc tttgattctt gaaaaactac gacgggtacg atgtttcccg tattgaacgt
    29221 catgtcctgc caagatgatt ctcctttgta aataagttcc aagccttgtc aatcaggctt
    29281 ttctaatcgt catatggttg taaacccctt atcaccgtgt cctcttgacg aattttcaga
    29341 atctttaagc ctctgttaca aatgctcaaa atcttgaaaa aaagcacaaa aagagcagct
    29401 aaatctgact ttttcagaag atttaactgc tgtgagcctt gtctggacaa tatttcagac
    29461 aaaacctacg acaaatgatt acccatatta taccctattt agctagattt ttcaaggggt
    29521 tttagtaggt ttttggtaaa ttttttccca tagaaaactt ggcatcacat tcgaatcacg
    29581 ctatggtaca aaaaactgaa aaaactattg actgaaaatc attttcaagg tataataata
    29641 aacgttaagg cggtatagcc aagtggtaag gcacggctct gcaaaagctt gatcgtcggt
    29701 tcaaatccgt ctaccgcctt ctataacttg atttatcagg tttcaaatga acagaaagcc
    29761 caatttgaag ggcttttttt attttccctc gaataaatac gtataacttt aaaaactttt
    29821 ggagcgagtt tgtggcagag ttctttccat ggcataattc ccttttgaaa tcaattgaaa
    29881 aaagcttcgt cacgcctggt gacaaaagcc tctagtttat tctgtttctt cttctatttc
    29941 gacttctgtg atttggactt ttaccttggt aacacgtcca ttcttcactt tatcattggt
    30001 taagataaac tgtttgtttt ggctaaccaa ttcatagctg agtttctcag tcgttggaat
    30061 agttcccact cctgtcaaat aataaccagc gatggtatca acgtcatcac tttccagttc
    30121 aacatcaaag taattattga aatcattgag ggtcatagtt ccttgaacaa tgtaggtatc
    30181 ctcgccaatc tgatgaacat ctattgctgc tttgtccgtt tcatcatcaa tttcaccaac
    30241 aatttcctct aagaggtctt ccaaagtcac caaaccagcc ataccaccgt attcatccag
    30301 caagattgcc atttgtcttt gggtatttcg cagttctttt agcaagtcat ccacaaaaat
    30361 agtttcaggt acaaaaagtg gatcttgcaa aattctcttc caaacaatat tgtcaaaacc
    30421 gtccacaaag cctgccttaa ggagactctt ggtgtgaatg attccaatta cattgtcctt
    30481 atccccatca taaaccggga tacgagaata attttgtttt aaaatacttt ggataatggc
    30541 ttgactatca tcctgaatat ccaccataaa ggcatccgtt cgaggaacca taacctctcg
    30601 tgccatcagt tcatcgagcg aaaagacacc ttgtagcatc tcaatctcat cagcatccaa
    30661 tgtttcttca ctatttgtca gcatataggc aatttcatca cgggtcatct tttcatccgc
    30721 atcatcgaat gacataggag tcaaatggct caagaaattg gtcgaagcag ctaaaagcca
    30781 aacaaaagga ctgactagtt ttccgatccc aatgataacc ggcgctgtac gaattgccaa
    30841 ggcatccttt agattaagag cgattctctt aggatataat tccccaaaaa cgatggaaat
    30901 ataggtcaaa aatgccaagg atagaaaagt tgccacggct tgtgctgttt cgccattccc
    30961 aagccaagag gcaatcacac gtcctagagt attagttaaa ctcgcccctg ataagattgt
    31021 aatcagggtg attcctacct gaatggttga taaaaagtgg ttaggatttt ctagtacctt
    31081 cagcaggcgg atgtagcgtc tgtctccttc ttccgccttt tgttcaactc gggcacgatt
    31141 aagagaaacc atggccattt ctgtggctga gaaaaaggca tttaataccg tcaagataaa
    31201 caataaaaca aattgtagca acaaattctg actacttggg tcttccatag ttcttctcct
    31261 aaaatagtat cttatccttt attatatcac aaaatttaat ccaatacata ttattttttc
    31321 ttaccgtaac ttctcaaagt aacttccatc tctctcccaa aactggaagt tagttttaga
    31381 agttgctatt ttttcttagt acctgttcta gccctgctta aactagtaaa aagaactcct
    31441 acatccataa gtgacatagg agtttaggct tatcttttac tgagttacag ttacttctcc
    31501 agttcggtaa tccagttgaa gtcccttttg aacattggga actagaacat tgaattccaa
    31561 ttctccatcc gaagacttgg cttcaatctg tgaagctgag ggaactaaat cctcgtttga
    31621 agcgtagtaa agggttacac ggtaacggac acgcttgttt tggtccaagg ctttacgcac
    31681 cttgctttca tagtagtttt gaccagtcga atactcggct tgtgcctgat ttgcccaggc
    31741 tgtctgaaca gcaatgtttt taggattgct tgttgaggca tcaaaaccat ccaaaccacc
    31801 gattaaggca tagcctaaca aatgacctct atcgactgca tgggtataag agccctttag
    31861 attcttgacc tgatgccaac ctggaggagt ccaagaagtt gaaccattcc cagtttcttt
    31921 acgattcttg tactgacgag tggccttaga caagagggca ttagctacgg ttggaacagt
    31981 ttccttgccc actgtctttg ttttattgtc agcgtagggc ttacttgaaa ccttggcatc
    32041 tagatttgtt ttattaccat tgacgataaa agcacctgag ccattccact ccagactccc
    32101 ctttatttga ctcttgactg cgtctgttaa gacactctct gccaatgctt gactaggagc
    32161 ttcagacgct tgttttttct gactaagatt ggttttggga ctattaggtg ccgacggcat
    32221 ctgcttgata taatagctcc ctgtagacaa aagcaataac actagcagtc cgattagtgt
    32281 ctgtcttgtt tttttgttca tatttctcct tatctttaga aaaaggctgg ttctccagcc
    32341 taatttcctg taaatttatg aatcaattcc tgccattttg ctggagacag gatagcccat
    32401 ggatcttgac ccttgcccaa gattccataa cctaccatta aaccgattcc tagagccaga
    32461 gtacctaaaa tcagtactat gatgactaaa agtaaacgct tgactacgta gcttgatttc
    32521 ttattcatct tcatcacttg cctcaatccg ctgaatctta gcacctagct gcgccaactt
    32581 ctcatggaaa ccgtagtaac ctctatccaa gtgaaccaat ttaccgacca cagtttctcc
    32641 ctgtgctacc aaacctgtca aaatcaaggc tgcactggca cgaaggtcag ttgaaagaac
    32701 ttctgctccc tgcaaaggct gtccaccaac aatacgagct gtatcacgga taatctcaga
    32761 atgcaagccc atgcggcgca tctcttctag gtgttggaaa cgattttcga aaactgtctc
    32821 caccatggtt gattcgcctt ttgcaactgt catcaaggct gtaaattgag cctgcatatc
    32881 tgttggaaat cctgggtggg gcaaggtttt cacatgaaca gcttttagat tttctagttg
    32941 agaacgaaca cgaattcctt cgtcttcttc aattacttca acacccattt caagtaactt
    33001 ggcaatcaag ggacggttgt gctcccagac agcgtctcga atcaagacat caccaccagt
    33061 catggcagca gctaccataa aggttcctgc ttcgatacgg tcttggacta cattgtgagt
    33121 cgtaccatga agtttctcaa caccagtaat ggttatagtc tctgtaccag cacctttgac
    33181 cttggctccc atttcattaa ggagaatggc taagtcaaca atctcaggct cacgcgcagc
    33241 attctcaatc actgtcaccc catcagccag agtcgctgcc atcatcaagt tctgcgttgc
    33301 accaacactt ggaaagtcca tatagatatg agcgccatgc aagcgttctg ccttggcttc
    33361 gatgtaacca gctgtctgac taatcttaac ccccatagct tccagacctt tcaaatgaag
    33421 atcaatagga cggctaccaa tcgtacaacc acctggcatg gataccttgg catgacccac
    33481 acgggcaagg attggcccta atacaacgat ggaggcgcgc atcttgctga catacttgta
    33541 aggggcttcc tcagtgatgt cgccagtagc atccaccttg acaagatgag cttcctcatc
    33601 aaagtcaacc ttggcattca aaccaccaac tacctgattc ataataaaga catccgacaa
    33661 aatcggaaca ttctgcaaga cggtctttcc ttcacttgct agaatagtcg ctgccaacaa
    33721 gggtaagact gcattttttg ctccctcgat cgtcacgctt cctaccagac gattatcgcc
    33781 accttgaacc acaattttat ccatactcgt ttcctttact cttgatttca taataatcct
    33841 ctaagcgctt cttgccacaa atgatacagg ctaataaaga aagaactcag gatgtagcct
    33901 atgacaatgc taaaaaaggc tactaataaa cgaacttttc ttgtattctc atctgtcact
    33961 tttaaaacct tctcccatct aacaagatcc tttaaaaggt aaaaactcac ataaataaag
    34021 agcatatgac tacttagagt gaataataat tgaaccattt gactattata ccatcttctc
    34081 tgtttgtgcg ctagtttgga aacttcactc aaaaattagg gattgttttt caagtaatca
    34141 atcgcatctt gtagggtttt aacgggcacg attttcatat ccgtcttgat tgttttagcc
    34201 gcttctaggg ctgtttggta gttgtttttc gcgtccggat gcgccttttg ttcttcttcg
    34261 ctaacagggt tatcaggagc aaagaaaata gcagcacctg ccctagccga agctacaacc
    34321 ttcttatcaa tacctccaat gtctcccaca ttaccatcgc ggtcaatggt acctgtaccg
    34381 gcaacaatac gaccattacg aagatctggg tgagctattt gagtatagat agctagacta
    34441 aacatgagac cagcacttgg accgccaata ccagctgttg aaaagctaat tgggacattg
    34501 ctgattacct ctgtacggtc aatcaagccg attccaattc catttttgcc attttccaag
    34561 gtgataatct ttccttctgc agacttggtt tgcccatcct cttcataggt gaccttgacg
    34621 gaatccccta atttttgaga actgacgtaa tcaatcaagt ctttggaact atcaaaggtc
    34681 tgatcattga ctgctgtgac tgtatcagag atattgagaa tccctttaaa ggttgaatta
    34741 tccgtcacat tcaaaacata aactccaaag tacttgagtt cgatatcctt accagctgtt
    34801 tttagtcctt gatacttggc catattttgc gatgtttgca tgtagaattg attgattcgc
    34861 ataaattcaa catcggaaga accacctgta gtctcctgag cactacgaat atctgtaaaa
    34921 ggtgtcaacc aagcataaat catatgagct aaagtggcat gttgaacacc aaccgtaacg
    34981 aattgatagg caccagcttc cttatcttct gtgtcattta ttttaaggac ttggcgaata
    35041 tcttccgaac cacctggaac ctctatataa taaggcaagg gcactacaaa tgccaagaaa
    35101 gtcacaatca aggccgcaat gacgtataag ggccatctaa tctttttttt catttcttat
    35161 ttcctccaaa atactctcgg gaacatagca ggcaatatcc cgaccaaact tcaaaagctc
    35221 tctaacgcct gatgaactga tatagagatg ttcaggtcga ctatgtaaat aaatagtctc
    35281 tatatcagaa gacagctgat gattgtagta atcaaaactg gcttcatatt gcaaatccga
    35341 cgcattcctc aaaccacgca ctagacaagt agcacccaat ctttttgcaa catcgaccac
    35401 caattcatca tgagaagcca cgacttcaac attttccaaa tgtcccaaag ccttttctag
    35461 cccccgttta cgattttcaa gagggagaaa tccttgtttg tggggattaa aaaaaatacc
    35521 cacataaagc ttatcaaaaa gtctgctcgc ccgttcaatg atatccagat gcccatttgt
    35581 catcggatca aatgagcctg tgaataagcc aatcttatct gacatagact gtcaccttac
    35641 taattccata aatcttttcc ttccagatac ccagacaggc aatttcttct ggaagttcaa
    35701 cggctttatc cgtctcgcac acaaccataa catcttcaga aaaaagctct ctctcagcca
    35761 ttttttcaat atctgctacg atttgttcct tggcataggg agggtctaag aaaacgaggt
    35821 caaattcccc agatacctgt tccaatgccc tttctgcatc catctttagg agttgaaatt
    35881 ttccaacttc cttggtcatc tggatatttt cagccacgat ggtctgagcc ttacggtctc
    35941 gctccaccaa aacagcactg gacatgccac gcgatactgc ttcgatagat aaaccaccac
    36001 tacctgcata aaggtccaag actcgtccca cttcaaagta gggaccaatc atgttaaaaa
    36061 tggctcccct aaccttatcc gaagtaggtc ttgtcgtctt gccttctagt gtcttgaggg
    36121 gacgtccccc atagattcct gatacgattt tcatactgtt tattatacca aattatagac
    36181 aaaaagagaa agaaaaccga accttgcggt tcgattctct acaaaatatt ttcgtaagta
    36241 tcgcggactt cttgaggcca aacacttgtt tgcacttctc cgatgtgtct cttgcgaagt
    36301 aggaacatgg ccatacgaga ttgtccaatt cctccaccga ttgtcaatgg gaatagacca
    36361 ttcaacaaag acttgtgcca ttccaattcc aagcggtctt catcacctgt aatttccacc
    36421 tgacgtctaa gagtttcttc atctacacga attcccatag aagacaactc aaaggctcca
    36481 cctaaagact cattccagac aagaatatca ccatttagac ccttgtagcc attctcagac
    36541 tcgcttgtcc agtcatcata gtctggtgca cgtccatcgt gcggtttacc atctggcaac
    36601 tcgccaccga taccaatcaa aaagacggct ccaaattctt tacaaatcgc attttcacgt
    36661 tctttcggtg tcaagtctgg gtagcgttct accaattctt ctgtatggat aaaggtgatt
    36721 tgttttggca agatagactc gatgtcatag cgggcttcaa cagctagctc agtcaggcga
    36781 atagccttat aaatcttctc aactgtttct tttagataaa cgatgttacg cttaccattt
    36841 gggataacct tctcccagtc ccactggtca acataaacag agtgggttgc atccaaggaa
    36901 tcctcatctg gacgaagggc tttcatgtgg acaaagagac cctctccttc accaaagcca
    36961 aaacgagcca aggtgtggcg tttccattta gcaagtgagt gcaccacttc ataagtagca
    37021 tcagggattt ggagaacctt gaccgatact ggattttcca caccagacag gttgtcctgc
    37081 attccgtcac cgaccttact caagatagga ccttgaactt cgacaacttc tagcttatct
    37141 ttcaaatact gggtaaaagt gtttttgaca aaggaaattt cttcttgttg atggataaaa
    37201 cttttcttca tagacaactc ctcaaaaatt taataaacgt attatacacc tatttccaag
    37261 aaaagaaaag agaaaattta aaaaaaatca gtgtcactca aaacactgat ttcatagacc
    37321 ttttagtcat tgcgaccaaa gatacgtaga atacttagga aaagattgat aaaatcaaga
    37381 tagatgctga gagccattga caccacccag cctgtcgcta cacgaccttg tgattgttca
    37441 taggcgaggc gaatcttttg gttatcccaa gcaatcaacc ctgaaaaaac caaaaccatg
    37501 gctacgctaa tcatataatc aaagaagcca ctagccaaga aaatattgac taccatagca
    37561 atcagtaaac caatgagagc tgccatcatt gctcgaccaa tcccactcaa gtctttcttg
    37621 gtaaaaatac caactgccgc catgacaaag aagagaaggg cgctcgatac aaaggcagat
    37681 aaaactgtac ccggagtata gaaagccacc acaaaactaa gggtaaagcc gtttaaaacg
    37741 gagtaaagta aaaatactgg aagagcagct ggactatttc ttgaagccat gctactggca
    37801 acgaaaacca aagccaactc cgcaaaagta gcaatggtca accagagacg cccctgcatc
    37861 aaaaagtaaa ccaactgaga ctgaaagacc gtcaacataa ggcctgatac caaagcagat
    37921 agtccgattc ccagaccaac aaaggcataa accttagcgt aaaattgatt gagacctgca
    37981 cggtcatgta taatagtgtg attcatccat ttctcctttt ctgtgaacat ctttccttga
    38041 ttataacaaa aaaagttaaa attagcttaa atggaagatt aaaataccgg ctgcaaccgc
    38101 aacattcaaa ctctcggcct gccccttcat gctaatatgg accaactggt ctgcactttc
    38161 agccataagg ggactaattc cttgaccctc atttcccata actagtacaa aattttctat
    38221 agaaggcagt tctctgtaat caacagaatc tttagatagg gttgttgcta gcactgggat
    38281 agctaccttt ttagtttcgt caagaagcgc ttgactagtc atccggtaaa tgggcagatg
    38341 gaaatgacta ccttgcatgg aacgtagggt tttgagactg tagatgtctg ccgacttatc
    38401 tgaaacaatc actcctgtaa aacctgctgc atccgcagtt cgaatgatag ttcctacatt
    38461 accaggatct tgcacatctt ccaaaaacaa gaacttgccc tgactcaaat cagcttgtcc
    38521 tacttcttct ttttgaacca cggcaacaat tccctgtgga gtctgagaat ccgccaaatc
    38581 tagcaaaata tcctctgaca cccagacagt ttgcgaaaaa gtagctaact gatctcgata
    38641 attttctagg gcaaagattt tctcaatcgt cactccagct tggacagctt cttcaaacaa
    38701 gtgccagcct tcaatcaagt aggcagactt gcggtatttt ttttggcgta atttcttggc
    38761 attttttacc acagaattgg cttttgaggt tataatagtc atagaaatat tataacacaa
    38821 tcaaaggggt ttggtatgca aaaggttaga atgattgccc aaggtagggt acagggagtc
    38881 ggctttcgtt ggggtgttta cagcttggca cttgaaattg gtggcatcac aggtcgagta
    38941 tggaataacg acgatggcac agtggaaatc ttagcccaag cagactcatc tgctatcatg
    39001 gcaaaattta tccaagaaat ccgaaaagga ccgacacctt tttcaaaagt aagctactta
    39061 gatgtcaaac taagcaactt tcctccctac tctgacttta aaatcgcaaa ttaggtctcc
    39121 agaactattg tatattttgt aaaaaaacag tagaatagaa aggtataatt tttaaagaag
    39181 gaataaaaac agtgaaatct attaaacgtt ttgcactctc agctatggga gcggctatgt
    39241 tgcttgtctt gactggttgt gtcaatgtcg ataaaaccac aggtcagcca acaggattta
    39301 tttggaatac catcggagcc cctatggctg aagctatcaa gtacttcgct actgataaag
    39361 gtctaggctt tggtgtcgct atcatcatcg taaccattat cgtgcgcttg attatcttgc
    39421 cacttggtat ctaccaatca tggaaggcaa cgcttcactc tgaaaagatg aacgccctca
    39481 agcacgtcct tgagccacac caaacgcgtc tcaaagaagc gactactcaa gaagaaaaac
    39541 tcgaagccca acaagctctc tttgctgctc aaaaagagca cggtatcagc atgtttggcg
    39601 gtgtaggatg tttccctatc ctccttcaaa tgcctttctt ctctgctatc tactttgctg
    39661 cccaacatac tgaaggggtt gctcaagcaa gctacctagg cattcctcta ggttctccaa
    39721 gtatgatttt ggttgcctgt gctggtgtcc tttactatct tcaatcgctc ctttcacttc
    39781 acggagtaga agatgaaatg caaagagaac aaatcaagaa aatgatttac atgagcccac
    39841 tcatgatcgt cgtcttctcc ctcttctcac cagctagtgt cacactttac tgggttgtcg
    39901 gtggtttcat gatgattctc caacagttta tcgtcaacta tatcgttcgt ccaaaacttc
    39961 gcaaaaaagt ccgtgaagaa ctagccaaga acccaccaaa agcaagtgct ttctctaaac
    40021 caagtggacg aaaagacgtt acccctgaac aaccaactgc tatcacaagc aagaaaaaac
    40081 acaaaaatcg caacgctgga aaacaacgtt cgagataaga agaaaaaggc ttagctgatt
    40141 tgctaagtct ttttgcaaat caaaagcccg atgtttccat caggcttctt ttctatccat
    40201 gtacacgttt catataatct tgataacttt cggtatccat gagtttttta gcgttcttga
    40261 cgcgatctgc tgttggtggt ttgactcctt cgagggaata tggaattcca agttcacgcc
    40321 acttgaactc acccatggtg tgataaggta gaatttcaaa cttatcaaca tttttgaggg
    40381 tcttgacgaa cttaccaagt tcaatcaagt catcatctct gtctgtcaat cctggaacta
    40441 gcacgtggcg aatccagaca ggttttccaa tatctgatag atactgggca caagccaaga
    40501 tatttttatt ggtttgtcta gtgacaatct tgtgctgttc ttcgttgatt tccttgatat
    40561 ccaaaagaac caagtcagtg acagccatga gtttgtcaaa cttctcaagg taacgtggtt
    40621 tattacggaa aggaagagca caggtatcca aggtacagtg gattccttgt tccttagcct
    40681 tggtgaagag agcaatcagg aaatcaatct gcaagagagc ttctcctcca ctgactgtaa
    40741 tcccaccctt atttccccag aaaccacggt agcgcaaggc ctctgtcaag acatcatcta
    40801 ccgtccgttc acgtgactta ttggactcca tagcccaagt gtctgggttg tggcaatact
    40861 ggcaacgcat gtgacagccc tgcaaaaaga caataaagcg aataccaggc ccatctactg
    40921 acccaaagct ttctgtcgaa tgcaccattc ctgtcacttg tccataatca attgtttctt
    40981 cagacatatc gttaccttcc ttgaaaacgt tttatagttt tattatatca cgacttgaag
    41041 gaaaaacaag atttttgcct attttttaga aagcaatctg tttttcaatt tcattttcaa
    41101 tctattatca gattattctt caaaatcgaa gccatataag gttgcaaaat agtcctcagg
    41161 gcgctctgca cggcggattt gacgggcttt accttcttcg gtatagagga tttccgcaga
    41221 acgtaatttg gcattatact ggtagcccat tgaaaatccg tgggcacctg tatcatgaat
    41281 taccagcaaa tcaccgattt ctgtatgagg cagttcgcga ttgactgcaa atttatcatt
    41341 gttttcacag agtgaaccaa ccacatctac cgcttcagct ggtccatctg gatgggtcac
    41401 gttgctaata tgatggtaag ctccgtacat agctggacgc atgaggttga ctgctgaggc
    41461 atccacacct agataggtac ggtaggtttc cttcttatga gtcactcttg tgactagagc
    41521 accgtgaggt gccagcataa aacgacccaa ttcggtgaaa atcttgacct gaccaagacc
    41581 tgctgacgta agaacttctt catacacctt acgaactccc tcaccaatca aggcgatatc
    41641 gttcggctcc tggtctggac gataattaac accaataccg ccagaaagat tgataaagtc
    41701 tagccaaatg cccaactttt ccttgatttc aacagccagt tcaaagagct gacgagccaa
    41761 ctctggataa tagagatggg tcacggtatt ggacgctagg aaggagtgaa tcccaaaagt
    41821 cttggctcct ttttccttca agatagcaaa ggcttcaaag agctggtcct tggtcatgcc
    41881 aaacttagcc tccccaggat tgtccataat gtctgtcccc agttcaaaaa cgcctccagg
    41941 attataacga caagagatga tttctggaat gcctgctact ctctccagat gttcaatatc
    42001 ttcaaaggca tccaagttaa tggtcgcacc caattcacgt gcataggcgt attccttgtc
    42061 tggcgtgttg ttggaagaga acataatctc agaacccaga aagtccagtt tatggctcat
    42121 caaaagctct acataactag agcagtccac accacaacct tcttcttgga gaattttcaa
    42181 aatagctgga gttggagtag ccttaactgc aaaatattcc ttaaagccct tgttccacga
    42241 aaaagcttgg ttgacggctc ttgccttctc acgaatcccc ttctcatcat acaagtgaaa
    42301 gggagtcggg aactcggcaa caatcgcttc taactcttct ctattgataa atggtgtttt
    42361 cataagcttc cttctattct gtttgcaaag aaaggctggg acaatcgttc caacctttag
    42421 ttctatctct tatttatctt cgtcaaagat attgccaacc tcaacatcga tggcttggtt
    42481 cttgacatca atattagtta ccttcaagag tgacttgtag tcaaactgct tttcacgttc
    42541 ttcttgggca ttgtccgcaa agaccgctac acctgccagt tctgagtcga actcgcgcaa
    42601 gagactaatc ataccgttga ccgttccgcc acctttcaag aagtcatcca caatcaagac
    42661 acggctacct gccttaagac tacgttttga aaggaacatt ttctcgatac ggtcaccact
    42721 tgaacctgaa acatagttga cgctaacagt tgaaccttcg gtaattttca ggtcacggcg
    42781 cacaatgaca aaagagacat tgaggacatt ggcaactgca tttgcaagtg gcacaccctt
    42841 agttgctacg gtcataaccg cgtcaatttt ttggtccata aagcttttgg caataatacg
    42901 accaatattt ttcaagatgg ctggtgtgct aagcaaatca gacagataga tataaccacc
    42961 tggcaagata cggtcacttt ctgacaactt ggtacgcaag tcttcaacca tttccttagc
    43021 atcctgactc gaaatagacg gtgtgaagat gacacctccg ccagccccag tcactgtctg
    43081 gatatgaccg atttcaattt cctcaaaggc gcgtttgata atgacgatat cttctgagat
    43141 ggatgattta gcagactcat acttttcagc aaaagtattg agactagtta gtttataagg
    43201 attattaatc aaatagttgg aaatgacaac catccgatca cttcttctta atttcatttc
    43261 tatttcctat tccattttta ttcaaaaaat caaaaagcaa actagaaagc tggtcgctgg
    43321 tggttcaaaa cactgttttg agattgtcaa tagaactgac aaaccctgta atatacctgc
    43381 atatatacat acgacaaggc gatactaccc tagtttgaag agattttcga agagtattca
    43441 tttttgtctt ttacttatta taccatattc acataaaaaa acgaacattc ttatcctaaa
    43501 aaatgctcat ttttcttaaa ttattaatct aaatctggtt tatagaagga acgattatcc
    43561 atagcgaaga ttttattggt catctctcct ttatccacca aagcaagagc tgttgacatc
    43621 atcatcatgc ttgcatccag attgtcaatc atatggataa tctctgcttc cataatgcgt
    43681 ggacggactg ggcttccata ctcaagcaag ccgtggtgac tgaggatgac atgacgaagc
    43741 aaaacgactt cttccttggt atcatcgatg ccgagttcca taactgtctt ggtaatttcg
    43801 ctatcaatga gagcgatatg tccaagaaga ttacctcgca ctgtgtactc tgtctggtct
    43861 ggccccgtca actcgatgac cttagctaag tcatgcaaca taatccccgc atagagcagg
    43921 ctcttattga gctgaggata aacttcgcta atagcgtctg ccaaacgcac catggtcgcc
    43981 gtatgatagg ccaagcccgt ttcaaaggca tggtggttgg tcttggcagc tggataggag
    44041 tagaattcct tatcatactt ggtgtagaga tttcggacaa tccgttgcca gacaggattt
    44101 tcaattttga aaatcatttg cgacatgtag tcacgaattt ccttgacatc aactggtgac
    44161 ttgaccttga aatcggctgg gtcattgggt tcaccagctt gaggcaggcg gagagtaatt
    44221 tgattgactt gaggggtatt gttataaact tctcggcgtc ctttcatgtg gacaacctta
    44281 cctgcggtaa aggcctcaat gttatgaggt tgggcatccc agagcttccc atcaatctcg
    44341 ccactatcat cttggaaggt aaaggctagg tagtttttcc cagctcgagt ttgcctcagg
    44401 tcagctgatt tgattaggta aaagccttca aatagctcat cttttttcat gtgactaatc
    44461 ttcatattct tcctcatttt cttgaaaatg gagtagatca agcgcaggct cgccttctga
    44521 caactcaatg tgacggagcg tccgctcgat agctatggta cgacggttta ataattcatc
    44581 aatattgcca gaggcatgtt ggagatgttt ttgtgccttg accagaatac caccaaactt
    44641 gccaaactcg gtcttgacac tggcaagagt cttgctgata tggtcggcac tcttttggat
    44701 attaagggtc ttgaaaccaa ctgataggga gttaagaagg gctgataggg tacttggtcc
    44761 tgcaacgata atctgttctt cccgtctcaa atcatcaaag aagaccggat tgcgcacaat
    44821 ctctgaataa agaccttctg tcggaacaaa caaaactcca aaattggtcg tccgaggcgg
    44881 tgctatgtac ttgctcttga tatccttggc aaagcgcttg acgcttgcta ggagtgactt
    44941 acgacagcgt tcaatctcat ccttgtcacc tgtctcatag gcttcttcca agcggtaata
    45001 atctgccagt ggaaacttag agtcaattgg cagatagacg tattcttggt cgccttgtcc
    45061 gggtaacttg atggcatact ccactcgttc actagagttt tcaaccgttg cgtattctcg
    45121 ttcgtactgg gcaggtgtca tgatgtcttc aataatttgc cccagttgca attctcccag
    45181 aatccctcgc gtcttggttc cagagagaac cttgttaaga gctccgacat cacgggcaac
    45241 tgtctgcatt tctccaaggc cacgattgac agactccagt tgtttagaaa ctgtctcaaa
    45301 ggaagcctgt aagcgtgtct gcaaggtctt ttctagtttt tcctcgaccg tctggcgcat
    45361 ttgttccaaa cgttgctcat ttgattcctg caaggcttgg agacgttggt ctgtcttgtc
    45421 tctagtttgg aggagattat ctgtcatttc ttgacggact tgggtcagac cttggtgcaa
    45481 ttcaatccgc acttcttgca aacggtcgct gacaaccact tccaaatctt tttggtctaa
    45541 ctggctggct tgtctggctt ggtcaaagcg gtaatccaac tggtctgaca aatgatctgc
    45601 ctgatcctcc aagctcttac ttaagtgttt ctcctgccta tcctgccttt gccaaatcag
    45661 aaagagacca gctaggttgg caattaataa tagaataagt aaactttcca tcctacctcc
    45721 tgtccttgct atgcagtacg accacataac catctgggca agttaccgac acttccctat
    45781 ctatatattc gttagaagcg tacacttttt taaagaaaaa attttcctct gtcaactcat
    45841 acttggctcc aagaatagtc agctggctat cccgaactgg cataaaggct agatagtcgt
    45901 agtctgaacg aggttctagc tgactggtcc cttctggaca ataagtaatc aagttttgcc
    45961 catcctcaat ttctatttga tgcatatagg gtgccaactt aggattgcta ggcagaaaga
    46021 cattggccaa catatggtca atacggccac ccaaggcacc gaaaatagta acctgagcct
    46081 gaggattttt ctcaaagatg gttaagagag ccaattccaa atcggtatca tctttttctg
    46141 gtcgggcttg gacaaaatgc tgggcacgtt tttgaatcac ctgtcgctct tcttcggtca
    46201 cagaatcaaa atccccaact gctagagcaa gaagcaagtc ttcttccaag acccagagcg
    46261 agcctcgatc caccccaaca aaagcatcaa aatctgtccg ataatgaccg cggtctccgc
    46321 ctgcaaaaac ggcaacccta gtccagttgt tttctgagag tttgtactcg ctcattgaca
    46381 tctcccttaa agacatagga acctgctaca aaaacggtcg caccagcttc tttggcttgg
    46441 gcaatagttt ggtcatcaat cccaccatcc acttcgattt caaagttcaa acctttttcc
    46501 tcacgaagag caaccaactc acgaacctta tccatggttt ctggcagaaa ggcttgccca
    46561 ccaaaacctg gattaaccgt catgactaaa acttggtcaa ctagatgaag gacgtgcttg
    46621 atggcttcaa ctgatgtgcc aggattgata acgactgaag gcttaactcc gagtgaacga
    46681 attttttgga gggcgccatg aatatgaggc gttgcttcta catggatact gatgatgtct
    46741 gcacctgcac gcgcaaaatc ttccagatga tgctcagggt ttgacaccat caagtggcaa
    46801 tcgaaaacca tcttactatg aggacgaagg ctctcgacca cacctgcacc aaaactgatt
    46861 tgcggtacaa aatgactgtc catgatatcg atatgggcat attctgcccc agttgcttct
    46921 agacgtttga tttcacgttc aaagttggca taatctgctg ccagaattga cggagcaatc
    46981 ttgtattgag acataggttt ctccttattt tggaattttt ttgctgactt ttttataggt
    47041 ttctctacga ttttcaattt cactaaggaa ttgcaggtaa ttgtcaaaac ggaaggttgc
    47101 aataacaccc tcttcaacag ctggtttgac ggcacaagac ggctcatggg tatgggtaca
    47161 agtacggaac ttacaatctc ggctaacagt agcaatctct ggaaaggcct gattgaggtc
    47221 ttcagccctt gatacttcat agtccagaga tgaaaagcct ggtgtatctg cgattttacc
    47281 cccgttgaga ttgtaaaaac taacagctcg agtggtatgg cgaccgcgac ctagactgtc
    47341 tgaaatttct cccgtttcaa gattgaggtc tggtgcgatt ttattgagaa gagttgactt
    47401 cccaacacct gtctgcccca taaagaccgt aaccttgcct gttaacaaag acaggagttc
    47461 ctctttactg gtcacaaagt catagccgat gtcaccatag gtctgctggt aaaaatccag
    47521 ttctccccta tcttccaaca aatccatttt ggaaatatag acaatgggat ggatgccctt
    47581 gtgctccaaa agaaccaaga aacgatccag caaattgctg ttaaaatcag gttccttgac
    47641 ggacatgatt actacagctt gatcgatatt gacaataggc ggacgaacca gactgttttt
    47701 ccgttcgtga attttgagga tatagccttc tgaattttcc tcggcagaga aatctaccca
    47761 gtccccaaca taaggggtat ggcctttttt acggaaattc ccacgcgcgc gtgtttgata
    47821 aacctggcca tcactctcca catagtagaa acctgccaag gctttaatga tttgtccctg
    47881 catcttagag tccttgccct ttaagtgcgt ctgctagacc ggcaaattct gccaagctga
    47941 gagcttcccc acgcacactt ggtgacaagc ctgcctggtc caaagccttg gtcagcttgt
    48001 ccttgacctc ttcagtctta ccaaagtaac ctgtcaagtt attccacaag gtcttgcggc
    48061 gatgggtaaa actagccttg gaaaccttaa agaaaaagtt ctcatcttct actgctacgg
    48121 ctggctctgg acgacgcacc attttcaaga tggctgaatc cacatttggc gctggcacaa
    48181 agaccgtacg aggcacgata aaggcaacct tggctgtcat gtaatactgc acggcgatag
    48241 acaagctacc gtaagccttg gtgttaggct gggctgaaat gcggtccgct acttctttct
    48301 gcatcatgac cacaaactca caaaaaggaa tgccactctc aatcaagtgc atgagaatag
    48361 gcgtcgtgat gtagtaaggc aaattagcca ctaccttgat tggcaggtta ggatttttaa
    48421 aattttggat atgttgcgcc aaatcaacct tgagaatatc ttcgttaact acggtcacat
    48481 tatcaaaatc acgcagagta tctgccaaaa ttggcatcaa acggtggtca atctcaaaag
    48541 ccatgacttg ggctgcacgc tcagccaaaa attctgtcaa ggcaccaata cctggcccga
    48601 tttcgatgac attgacctga tcatcaattt cagccgtatc cacaattttt tgaaggatat
    48661 tggtatccgt caagaaattt tgcccaaagg actttttaaa ggtaaaaccg tgacgctcca
    48721 acactgcctt ggtcacgcta taatctgcaa ttctcatcta ttctcttttc tattatctgt
    48781 tactactatt gtaacacatt ccccttgtat tttgggaatg aaaatcatac caaaaaacta
    48841 tctttcctca tgaagaaaga tagtcttgga aatctttaaa gagggactta gtgcttctct
    48901 cgaaacatac gttcatactc ttcgaaaatc tcttcaaacc acgtcagctt caccttgccg
    48961 tactcaagta cagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatca
    49021 aaaaaatcag cttcttgata acttcgattt tcgggtaggg tcaaatccac atcctcctcc
    49081 acatcatcca ctgtcagttc aaccgtctga ggtaaatttt tcaaacgata atgcttttgc
    49141 ctttgccctt caatactagc aaaatcaagc aaatagccat gcactcggat ttgaaccaaa
    49201 aggagatgat tggtcatatt ttccatgatg aggatatctt ctaaaccacc tctggcacta
    49261 ggataatcag ataccctgcg aatacggatt ttttcacaca tgagcaagtt tcctcatatc
    49321 aatgatttca tcagccttat tccagactag gggattatgg gtcgcaatga tgataattcg
    49381 attttcatct ttcaaatcca ccaagagatt cataacctcc tctgaatttt caggatcaag
    49441 agctgctgtt ggttcatctg ccaaaatcaa gggtggattt ttcaaaatag tcttagcaag
    49501 ggcaactcgt tgggcctctc ccccagataa agtataaatt ttttgttcta aatccaaata
    49561 ccctagatta actttttcta aagcccccac ttgcctttcc aaacgttcta cttttgagat
    49621 tttctgacca acaaaaccca aatccaaatt ttctttgatt gattggtttt ctaagaggcc
    49681 gaaattttga aagagatagc ccatctggtc tcgaaaatac tcacgagtag gaatggtttt
    49741 taaatctttc ccctgataga gaacccttcc accatctatc ttttctagct ttcccaagat
    49801 attcagcagc gtcgtctttc cgcttccact ctttccgatt aaggcataaa ccttgccctt
    49861 ctccagcttg agattcaaac cagagaaaat cgcgcggtca ttaaatttct tttccaatcc
    49921 ttgaatatca atcatcttat tttcctttca atactgccat gctaccagct tcttcttttt
    49981 tatcctgcct tactaaaatc aagagggcgt taagcgtaaa caaagctaca actagagcgc
    50041 taatcaaacc atctcttgtc aaaatactag ataggactag gccaagcaag agaactcctc
    50101 cttgcgccag taaatacttg ccatgaagct catagattgt cataccagca agacgtttaa
    50161 tcataagttc ccgtctgaac tgctcaaaat agagaagatt catggaatca aataacaaga
    50221 tagccgtaga cagggtcaaa atcgtcccaa taaggagaga gtaaaactcc acccgtttgt
    50281 catttagtac cttggcaaaa aatagctggc tttttaccaa gtaagaaacc ttgtgataca
    50341 gaccttgctc ttttagagct gtgatgctgt ctccatatcg atctagtttc aaggcactat
    50401 tagccgtagt tccccacaac atccctgcaa caggtcttgt tccagtcgct tgcggcgtta
    50461 gaactacaat aatcggatct tttaggtact ggcgtgttgt cttgtaccca tcattataga
    50521 ggaaacgttc ctgtcctgtt tctgtaaaag ctagccttac ctgtgggagg tagtgtttct
    50581 gactcgtcac ttctacactt tcagatgaaa agttttgcag gtaatctgta aacaatcctt
    50641 ggtagtaggc agactgctct cgcaagctct caggcaagat cagcccaaac tctccctcag
    50701 acaagttttg catcttgtcc ataaactctg aagaaacggt aatcttttct tctatcagat
    50761 agcgcggcga gacatagata acattcccta acggtgtgta gtcactgaga cgattgccat
    50821 ctaggtccac ttctgctcca tctgagaaat tgtcaacatt gctcataata taaaaagagt
    50881 ctgtattggc taaccgttct tcagtaaatg tctgccactc acgattatcc ttacgcgttc
    50941 cttcttcatc ggcaaatgca ctagaccaac caaaggatag acggtaacgg tctgaggact
    51001 ggctccattt attgctagct ctctccattt cctgcatttc acggtagtgg ggtaggagag
    51061 ctgtcgcact cgatccgacc accaatacag ctaagagttg ccccaccatc atcaatgtca
    51121 tcatacgttt gagagggagt ttccctttca atagatccac cagactattt tcctgtaaac
    51181 caagtagata gacgacactt agtaaggtag aaatccctgc caaggtcaat ccataaagta
    51241 gaagagcaat gatgaccagt tgtaccgttg ccataaacaa ggcaccttga taccagagaa
    51301 tccccaatcc caaaagactg gataccagca ctgagcagat aagctgtctc acatcttcta
    51361 acactggtct gagagcaact ccaaacaagc tctcaccagc tattaagcga atccctgcct
    51421 gacgaaggga tttgatccga taaatcaggg tcagactcat aaaggtcagc agaaaaatag
    51481 ctaaactcag tagcaccata ggggtggccg ccaatagtaa gactatcgaa aatggatctt
    51541 ctccattcga aacaaagcct tgataaccaa gctctttcaa ggtggtctgt aagctcactc
    51601 catccaaact tcctgacaca atcaagtaag accctaataa atcactcgtt tctgcactct
    51661 ccttggagga aatggtcaga ccttctggaa gctctccctc accataagtg gcataggtaa
    51721 agagtgtttc tccagcttca tttggctcaa cgattcgacg ggcgatgaga ctattatgct
    51781 ctaccgccaa gtgactcaaa gcctctgtca cctcttcgcg actagctact ggttgattta
    51841 tcatcattcc tgtcacatca acagaaggat agttaccaaa taaaatctct tctcgatggt
    51901 caacggaagt tatcaccata taaagcgata ccaataccat tgaaatcaaa ataaataaac
    51961 gtttcatcat ttcctccaaa aagttacttg gtcacttagt gagaaggtga ccaagtaaag
    52021 ctaaacaata gggctatacc attctaaaca ttatagtaga atgcaacttt ctctccccaa
    52081 gaagtacgaa tccatgcacg agaagtccct ccagcaccag catatccctt gtcagatgaa
    52141 ccagtttttc tgctaacaac tgttgaacta tggtattttg aagggtgata gtagtttgag
    52201 taagcaccac cgttacctga accataagtc caaacaccac cttctgggta ttgaacggct
    52261 gcagaggcaa ctgatacacc tccacctaat gttgctgcaa tgctcgctac aagagctaac
    52321 tttttaactg tttgtttcat attgatactc ctctattttt gattacaaga ttgttgagtt
    52381 acttgctcct ctttccatta tcaacgttga tactattata ttaccaaatg atattctaaa
    52441 attatttgtt tttctctaaa ttgtcaaaat agcaccctga aatgaacttt tatagtcatt
    52501 atactccatc tttatcacac tatcaaggac atcaatgtcc ttaaaatcta actatttcat
    52561 attattttta gcttatatct aaaaaaacag ttctaaagac gaactttaga actgttttga
    52621 tacgaggaca aggacatttt tgtcactgat tcactatgtt tataaatatt ttttgacatc
    52681 ctcttgccat tcctctgtca atttctttac tagaaatcca tctccaatta gcttcgcaaa
    52741 tgttttagaa gactgataaa atgattcagc taaactaaaa tctctttcta ctcttaatgc
    52801 ttgcttccac aaaaacataa atacaagagg tttcttttga taatcttgag ttttatccat
    52861 aatttcttgt aaacacgata gataatcatt aaaacaatca gttactccta ccttttcaag
    52921 acaacacatt cctgaaataa gagtatctct aattataaag aagttttcta atttagttac
    52981 ctttttctgt tccaaaagaa agagcattag cttttcgatt tgatttataa atttggcaag
    53041 atcttttcta atgagcattt gataaaaaaa caaacgaatc aacaacatat catttgttga
    53101 ataaacttct ttttctatga tctgagacat atgttcctca agtattaact ctgcatattc
    53161 aggccttcta gttctagaag tatccaaaca cgcctgcaat acttcacatg ctaattgttc
    53221 ttctttagga agattatcaa agtatgtttt ataaatctct tccaaacacg cttctttctt
    53281 atcatactcc tcttctttac catagattgg ttcacgtaaa atctggtatt tcaattctaa
    53341 ataagcagaa ggaagagctg aaaaatccgg cataaggcta taaactggaa ctcctagccg
    53401 gcgagcaata taggctaact tgggcaaact cggttgcgaa gctccacttt caattcgcga
    53461 cagttgacga actgtcagtt cttgctcatc tccacaaaaa tctggacgac taattccttt
    53521 ttctatccga atctctttaa ttttttgacc aattaacatc agacgcctcc tcttctacct
    53581 gcttcgaaaa atactgtgaa acttaaaaaa ttgtaacggc tacatctttc ctactcataa
    53641 gatttcatag cttcttccac ttctgccaaa gtaaccccaa acaactctag gcgtttgagg
    53701 agttgcttgc cattagaata gccgattcgg agagtctctc cgagatattc tctacgctta
    53761 cggctgtctg cccctgctag aaaaccaagg cgaatcaagt cgctacgact aatgtcaaac
    53821 tgactctcat gttcaaattg ttctgtcacc tgagctagag ccgttttcag gtcttcatag
    53881 ctggcatgct caattcccag agaacgcccc ttggtcttgg acttgggaac agcttcatct
    53941 cgcttgagaa aggcatgctg aactgttgga atggccgtca taatcatgcg ccgaatccgc
    54001 tccccattaa aatctgggtc tgtaaagaca atgactccat gacgttggtg tagacgctga
    54061 attcgctcta tatcctgatc attgatggca gaacctcgtg tctcataggt ctccacatcg
    54121 aaataacgct tgagattgac cgtatcatag cgaccttcaa ccacgataac ttgagaaatt
    54181 ttctctttca ttacttgctg tccaatccaa aaattcgttc tgcatttgca gtcgttgcta
    54241 ccgccagctc ttctgtcgtc ataccacgca agtcagcgat aaagtcgacc acatagcgag
    54301 tataggctgt tttattttca cgaccacgct tgggtacagg tgctaagtaa ggcgcatctg
    54361 tttccaccaa catcttgtcc aaaggtaact ctttagctgc ttcttggagg tcagttgcct
    54421 tcttgaaagt caccactcct gagaaggaaa tggtcatacc aagatccaca aacttctctg
    54481 cccactcaag cgtccctgaa aatgaatgca tgataccacc acgaggacca acgccctcac
    54541 tcttgataat ctcataggta tcttccagcg catcacgggt atggacaaca aaaggcaaat
    54601 ccaagtcctt agatagctga atctgacggc gaaaaacctg ctcctgcacc tctttgggcg
    54661 ctgtcatcca atggtagtct aagccaattt cacctaaagc cacaaccttg gaatgtttta
    54721 acttatccaa caagtaagcc tcaacttcct ctgtataagt accagcttct gtaggatgcc
    54781 aaccaatagt cgcatagagc tgctcatact catctaccaa ctccaaggca cgctcaatcg
    54841 tcggtttatc aaaaccaaca atattcatct gtatcacacc catctcagca gccaaggcaa
    54901 tttcttctgc ctcacgacct gcaaattctt ctacattcaa gtgtgtatgt gtatcaaaaa
    54961 tcatctcttc taacctcgtt ttctatcttc tattatacca aaaaagagga ggggcaccta
    55021 atttttcggt ttcccctcct ctcttcaata gagagctatt ctgctatctt ttctatccga
    55081 tattgcccat ctcctattcc acagttagag acagaagaga ttggctacat tgatagaata
    55141 ttcctattca gctaaagtat gtgattatct tgggttctca cttttctcac ccatcactag
    55201 gattgatgac ttctcaaaat cccccttata atctaacttt aagtagagaa tagaattttc
    55261 tagctgttct attttctacg tctagtcttt cttcctagca aagcaaaacc acttgttgcc
    55321 accactagac caagactagc aagtgctgca cttgatgctg aacctgtgtt aggtaattgt
    55381 ttgccagcag gtgactggta ggtaacatct tgcattgttc ctgcagttgc ttgcggtgca
    55441 cttgttgtct taggagcagc attaggagcc actggtctct taggaactgt aaccatggca
    55501 atggcttgca ctccaatcgt ttctgcttcc ttcacatctt ccatagtttt cgcattttca
    55561 atctctttga gagctgcttg tttttctgct tccactcttg ctaaaagttc tgctttttct
    55621 ttatcagaaa gcggtgcgcc tttgatttca tcctgcttat ccttggctgc tttttcaatg
    55681 gcatttttag ctgattcttt ttcagtagct aattgcttag aagcttgtga ttgagctgag
    55741 ttttgtgaac ctgtgtgagc tgatccaccc gcctggctac caccgccttc attcgcatta
    55801 gcatctgagc ttccagtatt gcctccctta tctccacctg gtatagtttc tagcttatac
    55861 tctagtgtag cttcttgagt tacagattca cctttcgcag attgttgaac tgtatctttt
    55921 cctagaatcg tcactactga accatctggg aatgtgattg ttgctgtacc atctccagct
    55981 acattgattg tcgctccgtc taatgctgaa ccatttgctt gtaaaatagc aaccttaact
    56041 ttttcttcat ctgttaaatg agttatatct tttacatcta ctctttgagc aggtgcttta
    56101 acagttgatt tttcagcagt cggtttagtg ctatcttgtg cttttggatc ttgtgctacc
    56161 aattgatttc ctggaattgt aatagctgaa ccatctttaa agacgattgt tgctgtacca
    56221 tctccaccta cttcaacttt atagtaaaat aaaataataa caggacaaat cgatcaggac
    56281 agtcaaatcg atttctaaca atgttttaga aacagaggtg tactattcta gtttcaatct
    56341 actataacat cgcttccgaa tccatttgca tctactgaac ttttttacaa aaataaaaat
    56401 cagtagactc gagttcctac tgatttttga tagaattcgt ttggattatg cagccaaaac
    56461 tttgcgtcct ttacgacgac gagctgccaa tacgcgacga ccatttttag ttgacatacg
    56521 gttacggaat ccgtgtttac gcgcacgacg aagtttactt ggttgataag tacgtttcac
    56581 gatgaatacc tcctcataga ttttgtattc gtttagccgg ctataaagtg atcagttact
    56641 aaacatactt tactattcta tctgattctt tcacttttgt caatagctct aggcaaatgt
    56701 ttccagcttt ttcgaatgca agccct
//
LOCUS       DBEJAQ010000006        49409 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.006, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000006 DBEJAQ010000000
VERSION     DBEJAQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 49409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.006"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..1841)
                     /locus_tag="ACL297_000421"
     CDS             complement(<1..1841)
                     /locus_tag="ACL297_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGR0070743.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYTQYILAAGYLNRVN
                     DIWLENEENVENGLKKCSLEGRIDIKDLSFSYSKDSAPVIENLNLTIEPGQRIALVGQ
                     SGSGKSTLSKILSGLYKIDTGKILFDGVNINQIDKKILSQNLGVVPQDSFLLNRSILD
                     NITLKNEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGG"
     gene            complement(1834..2382)
                     /locus_tag="ACL297_000422"
     CDS             complement(1834..2382)
                     /locus_tag="ACL297_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGR0070744.1"
                     /translation="MSLSYYYEINPSTDILKCIEELLYKEDKCFNNILKNWKDIRRNH
                     NDSFPNFWCYGAPGILLARKEIFDKTNIGNNDLSIIKNVLTNVEKIRELNLCHGSVGT
                     ISCLDAILKDEENLLIKESIDFYFDNVVSQVIKPELSTDLNTMNTFSFMLGVSGVVYE
                     ISRKQDDRLLNVLLLELRGHDD"
     gene            complement(2463..2525)
                     /locus_tag="ACL297_000423"
     CDS             complement(2463..2525)
                     /locus_tag="ACL297_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HGR0070745.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            2605..3459
                     /locus_tag="ACL297_000424"
     CDS             2605..3459
                     /locus_tag="ACL297_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGR0070746.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKIEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIDKEEFSNCSYLISKIK
                     TLLDNELNFYEQTVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGENKLKLHYTDH
                     FNKLVNKK"
     gene            complement(3504..5389)
                     /locus_tag="ACL297_000425"
                     /pseudo
     CDS             complement(3504..5389)
                     /locus_tag="ACL297_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775543.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
     gene            complement(5412..5741)
                     /gene="mobC"
                     /locus_tag="ACL297_000426"
     CDS             complement(5412..5741)
                     /gene="mobC"
                     /locus_tag="ACL297_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HGR0070747.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQV"
     gene            complement(5751..6110)
                     /locus_tag="ACL297_000427"
     CDS             complement(5751..6110)
                     /locus_tag="ACL297_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HGR0070748.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            complement(6942..7319)
                     /locus_tag="ACL297_000428"
     CDS             complement(6942..7319)
                     /locus_tag="ACL297_000428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070749.1"
                     /translation="MGQKVVAMLDFLLAYSDYSKDFRPLIIDQPEDNLDNRYIYRHLV
                     QQFRDVKAQRQIILATHNATIVTNSMTDQVVIMESDGVNGWIESQGYVSEKYIKNHII
                     NQLEGGKDSFKHKMSIYETALSE"
     gene            complement(7322..8098)
                     /locus_tag="ACL297_000429"
     CDS             complement(7322..8098)
                     /locus_tag="ACL297_000429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070750.1"
                     /translation="MRGFNNKIKSVYQELTNSKEKFGSFHKTLIHLHTPVSYDYKLFS
                     NWTATKYRKITEDELYDIFFENKKIKVDKTIFFSNFDKVVFSSSKEYISFLMLAEAII
                     KNGIEIVVVTDHNTTKGIKKLQMAVSIIMKNYPIYDIHPHILHGVEISAADKLHIVCI
                     YDYEQESWVNQWLSENIISEKDGSYQHSLTIMKDFNNQKIVNYIAHFNSYDILKKGSH
                     LSGAYKRKIFSKENTRFWSLILTRKNFRNNLIFSIKKLVY"
     gene            complement(8126..8887)
                     /gene="pezT"
                     /locus_tag="ACL297_000430"
     CDS             complement(8126..8887)
                     /gene="pezT"
                     /locus_tag="ACL297_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HGR0070751.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELFFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(8887..9363)
                     /gene="pezA"
                     /locus_tag="ACL297_000431"
     CDS             complement(8887..9363)
                     /gene="pezA"
                     /locus_tag="ACL297_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HGR0070752.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            complement(9808..10263)
                     /locus_tag="ACL297_000432"
                     /pseudo
     CDS             complement(9808..10263)
                     /locus_tag="ACL297_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10538..12277)
                     /locus_tag="ACL297_000433"
     CDS             complement(10538..12277)
                     /locus_tag="ACL297_000433"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HGR0070753.1"
                     /translation="MELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIASQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEINSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELD
                     NQLVISPDGFMIF"
     gene            complement(12440..13324)
                     /locus_tag="ACL297_000434"
     CDS             complement(12440..13324)
                     /locus_tag="ACL297_000434"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGR0070754.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKVL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(13436..14290)
                     /locus_tag="ACL297_000435"
     CDS             complement(13436..14290)
                     /locus_tag="ACL297_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGR0070755.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(14290..15105)
                     /locus_tag="ACL297_000436"
     CDS             complement(14290..15105)
                     /locus_tag="ACL297_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGR0070756.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNETHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKMKFLKSNRMLFSKINMVDNQYKKHQNEQGGI"
     gene            complement(15167..15355)
                     /locus_tag="ACL297_000437"
     CDS             complement(15167..15355)
                     /locus_tag="ACL297_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGR0070757.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(15677..16243)
                     /locus_tag="ACL297_000438"
     CDS             complement(15677..16243)
                     /locus_tag="ACL297_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070758.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGVEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(16511..18190)
                     /locus_tag="ACL297_000439"
                     /pseudo
     CDS             complement(16511..18190)
                     /locus_tag="ACL297_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(18282..18443)
                     /locus_tag="ACL297_000440"
     CDS             complement(18282..18443)
                     /locus_tag="ACL297_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070759.1"
                     /translation="MNNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(19127..19426)
                     /locus_tag="ACL297_000441"
                     /pseudo
     CDS             complement(19127..19426)
                     /locus_tag="ACL297_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19407..19796)
                     /locus_tag="ACL297_000442"
     CDS             complement(19407..19796)
                     /locus_tag="ACL297_000442"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGR0070760.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(19849..20033)
                     /locus_tag="ACL297_000443"
                     /pseudo
     CDS             complement(19849..20033)
                     /locus_tag="ACL297_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612340.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(20184..20978)
                     /locus_tag="ACL297_000444"
     CDS             complement(20184..20978)
                     /locus_tag="ACL297_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070761.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
     gene            complement(20978..21985)
                     /locus_tag="ACL297_000445"
     CDS             complement(20978..21985)
                     /locus_tag="ACL297_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HGR0070762.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(21985..22992)
                     /locus_tag="ACL297_000446"
     CDS             complement(21985..22992)
                     /locus_tag="ACL297_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGR0070763.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(22982..24007)
                     /locus_tag="ACL297_000447"
     CDS             complement(22982..24007)
                     /locus_tag="ACL297_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGR0070764.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            complement(24400..24618)
                     /locus_tag="ACL297_000448"
     CDS             complement(24400..24618)
                     /locus_tag="ACL297_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0070765.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(24864..25142)
                     /locus_tag="ACL297_000449"
     CDS             complement(24864..25142)
                     /locus_tag="ACL297_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070766.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(25356..26987)
                     /gene="rlmD"
                     /locus_tag="ACL297_000450"
     CDS             complement(25356..26987)
                     /gene="rlmD"
                     /locus_tag="ACL297_000450"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGR0070767.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLDMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(27061..28035)
                     /locus_tag="ACL297_000451"
     CDS             complement(27061..28035)
                     /locus_tag="ACL297_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGR0070768.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(28109..28723)
                     /gene="pmp23"
                     /locus_tag="ACL297_000452"
     CDS             complement(28109..28723)
                     /gene="pmp23"
                     /locus_tag="ACL297_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HGR0070769.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVQLNLYIIKCFTLFSTSG"
     gene            complement(28723..29697)
                     /locus_tag="ACL297_000453"
     CDS             complement(28723..29697)
                     /locus_tag="ACL297_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGR0070770.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(29705..30961)
                     /gene="glyA"
                     /locus_tag="ACL297_000454"
     CDS             complement(29705..30961)
                     /gene="glyA"
                     /locus_tag="ACL297_000454"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGR0070771.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(31023..31454)
                     /locus_tag="ACL297_000455"
     CDS             complement(31023..31454)
                     /locus_tag="ACL297_000455"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070772.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(31456..32058)
                     /locus_tag="ACL297_000456"
     CDS             complement(31456..32058)
                     /locus_tag="ACL297_000456"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGR0070773.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(32042..32881)
                     /gene="prmC"
                     /locus_tag="ACL297_000457"
     CDS             complement(32042..32881)
                     /gene="prmC"
                     /locus_tag="ACL297_000457"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGR0070774.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(32881..33960)
                     /gene="prfA"
                     /locus_tag="ACL297_000458"
     CDS             complement(32881..33960)
                     /gene="prfA"
                     /locus_tag="ACL297_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGR0070775.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(33957..34547)
                     /locus_tag="ACL297_000459"
     CDS             complement(33957..34547)
                     /locus_tag="ACL297_000459"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGR0070776.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            34670..34852
                     /locus_tag="ACL297_000460"
     CDS             34670..34852
                     /locus_tag="ACL297_000460"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HGR0070777.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            34988..36361
                     /gene="mnmE"
                     /locus_tag="ACL297_000461"
     CDS             34988..36361
                     /gene="mnmE"
                     /locus_tag="ACL297_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGR0070778.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(36408..37343)
                     /gene="dapA"
                     /locus_tag="ACL297_000462"
     CDS             complement(36408..37343)
                     /gene="dapA"
                     /locus_tag="ACL297_000462"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGR0070779.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(37399..38475)
                     /locus_tag="ACL297_000463"
     CDS             complement(37399..38475)
                     /locus_tag="ACL297_000463"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGR0070780.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(39106..39178)
                     /locus_tag="ACL297_000464"
     tRNA            complement(39106..39178)
                     /locus_tag="ACL297_000464"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39143..39145),aa:Thr,seq:ggt)
     gene            complement(39278..39787)
                     /locus_tag="ACL297_000465"
     CDS             complement(39278..39787)
                     /locus_tag="ACL297_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGR0070781.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(39785..39881)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            39910..40341
                     /locus_tag="ACL297_000466"
     CDS             39910..40341
                     /locus_tag="ACL297_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGR0070782.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            40423..40800
                     /gene="mscL"
                     /locus_tag="ACL297_000467"
     CDS             40423..40800
                     /gene="mscL"
                     /locus_tag="ACL297_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGR0070783.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(40842..41936)
                     /gene="hemH"
                     /locus_tag="ACL297_000468"
     CDS             complement(40842..41936)
                     /gene="hemH"
                     /locus_tag="ACL297_000468"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGR0070784.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            42091..43314
                     /gene="pepT"
                     /locus_tag="ACL297_000469"
     CDS             42091..43314
                     /gene="pepT"
                     /locus_tag="ACL297_000469"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGR0070785.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(43350..44003)
                     /locus_tag="ACL297_000470"
                     /pseudo
     CDS             complement(43350..44003)
                     /locus_tag="ACL297_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
     gene            44001..44165
                     /locus_tag="ACL297_000471"
                     /pseudo
     CDS             44001..44165
                     /locus_tag="ACL297_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(44436..45447)
                     /locus_tag="ACL297_000472"
                     /pseudo
     CDS             complement(44436..45447)
                     /locus_tag="ACL297_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1,RefSeq:WP_007521835.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(45673..48792)
                     /gene="phtE"
                     /locus_tag="ACL297_000473"
     CDS             complement(45673..48792)
                     /gene="phtE"
                     /locus_tag="ACL297_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HGR0070786.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(49002..>49409)
                     /locus_tag="ACL297_000474"
     CDS             complement(49002..>49409)
                     /locus_tag="ACL297_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HGR0070787.1"
                     /translation="ADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLYKPS
                     TDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLTGLK
                     SSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
BASE COUNT        14707 a         9801 c         7887 g        17014 t
ORIGIN      
        1 tgcccacctg aaatatttga ccccatctct gagatgatag tattaaattt catcggcata
       61 gccatgattt catcatagat ttgaactgct ttacaaactt cctctatctt ttgtgaagta
      121 acttcgttct ttaaagttat attatcaaga atacttcggt tcaataaaaa tgaatcctgt
      181 ggaactactc ctaaattttg acttaatatt tttttatcta tttgattaat atttacacca
      241 tcaaataaaa tttttcctgt atcaatctta tataatcctg atagtatttt tgataacgta
      301 ctttttcctg accccgattg accgactagt gcaattctct gtcctggctc aatagtcaaa
      361 tttaaatttt ctattacagg agctgagtct ttggaataag agaaagatag gtccttaata
      421 tctattctac cttctaaaga acacttttta agaccatttt caacattctc ttcattttca
      481 agccagatat catttactct atttaagtat cctgctgcca aaatatactg cgtataagca
      541 ttaaatattg agatttctga actaaataaa attgatgcca tggtttgaaa tgcgacaacc
      601 tcacccaata aaatttttcc atttaaaaca ttatcaagtc caaaaatcaa tataattata
      661 ggagcgaaca attgaaatac attagtagct gaattgtata aaatatgcac aatacttcgt
      721 tttttatact ttgtcaatac gttttttaaa tacttagacc agttttcata tatttcatct
      781 tctaatcccg atatttttat tgaagaaata gtatttaatg cttctgtttc tactgagtaa
      841 atcaatgatt gttcttgtaa ttctctatta accgtatcat acattatttt tctagtacta
      901 aacagaaaca aaaaattgat taaactcaat ataagagcta taattgataa tgtttttgaa
      961 ctaagaaaca tatatattac aacacttaga atagtcccga tatctactac tcccccaaca
     1021 acttggttag taaatagctc cctaaaacca ctaagactag aaattctgta taaaatatct
     1081 ccttttgccc tgagttcaaa aaaactatat ggtagtttaa gcaaatgctt aaatgtaaaa
     1141 ctttcaatat ctctactaaa aaatatatta gaaaatagaa ttcccatact cctagccaag
     1201 atagaaataa gatacaaaca aagtagagaa aataaaatta aaaacgaggt ttgaaaagaa
     1261 aatgtattcc ctaatgcaga attaataaac tttgaagtca ttactggtac ggaaagtatt
     1321 attaagtaac ttattattga aaatagtaac gtcaaaatca atcgaccttt tacctttgaa
     1381 aaactagaga atactcttat ccatggagaa ggaaactgcg acttcaaaga ttgataattt
     1441 tcgttaggaa atgatagcag tactatatta ctaaaatgtt ttgaaaactc cttaaactca
     1501 taatttatat atcctttaga aggatccatt atatagacct tattcttttt tactttgtaa
     1561 ataactacat aatgttgatt atcccagaat gctattaatg gtaacggaag ttcactaatt
     1621 ttatttacat ctttaacttg atatgagcca gtatcaaaat ttcgtttatt caataactgt
     1681 ttcaagtcac ctatactata accatctctc cctttttcta tatcacgtct taaatctagt
     1741 aatgtctctt tgcttttata gaaatgtagt atggacaagc agcaacataa accacattct
     1801 atttgagaca tttgctcaat attaggtact tttctagtca tcatgtcctc ttaactccag
     1861 taataaaaca ttaagtaatc tatcatcttg ttttcgagat atttcataga caactccaga
     1921 tactcctagc ataaaagaaa atgtattcat agtattcaaa tccgtactta attctggttt
     1981 gatgacctga gaaacgacat tatcaaaata aaaatctata gattctttta ttaataggtt
     2041 ttcttcatct ttcaatattg catcaagaca actgatagta cctacagaac cgtgacataa
     2101 attcaattct cttatttttt ctacattagt taatacattt ttaattattg ataaatcatt
     2161 attcccaata ttagttttat cgaaaatttc ttttctagct aataaaatgc ccggagctcc
     2221 ataacaccaa aaattaggaa aggaatcatt atgatttctt cttatgtctt tccaattttt
     2281 taatatatta ttgaaacatt tatcttcttt atatagtaat tcctctatac attttaatat
     2341 atctgtacta ggattaattt cataataata tgataatgac attgctactc cagaataacc
     2401 gtgtgctaaa cctgttactg ttatattatc taattgtgaa atacaataat taatacactt
     2461 ttttaaatga tgataatatc catcaccaaa accaaaatcg gtaaaaattt tgaaattttc
     2521 ttcatacttg gacttccttt ccgtttttat ttattgtaca ataaataaaa acggatttaa
     2581 tttttgttgg gaaaatggga aattatgaaa tcaaaacttg gcatcacact tagaaaagtt
     2641 cgtaaaggaa aacaaattag tctatgctct gtagcagatg aacatctttc aaaatcacaa
     2701 atatctcgtt ttgaacgtgg ggagtcagag atatcttgta ttcgactaat caatatttta
     2761 gacaaattac atattacttt ggatgagttc cttgttttac acaatgacga ctataccagt
     2821 tccgaatcat ttgctaatct agtacagtac attcgtaagc aatattcatc acaaagtatc
     2881 aataatatag catgtctact ttctgatact tctgattaca ctctaaattc ttttgagaaa
     2941 acaatggtaa aatctatttt acacacaatg gattcgaaca tcattccatc agatgaagag
     3001 cttcttcatc tgacagacta tcttttcaaa attgaaaaat ggggctatta tgaaatcatt
     3061 ttattaggta actgtgtacg cacaattaac tacaactcct acttcttatt aactaaagag
     3121 atgttaaata actatattta ctcatctctg aataaaacta ataagcagat tgtctctcag
     3181 cttgctatta attgcttcat tctaagcatt gataaggaag aattttctaa ttgctcctat
     3241 ttaattagta agataaagac attgttggat aatgaactaa acttttatga acaaacagtt
     3301 tttctctatg caactggtta ctttgaattt aaacgttgcc aatcaaccag tggaattgaa
     3361 aaaatgaaac aagccataca agtcttcgat attttaggag aaaataagtt aaaattacac
     3421 tatactgacc attttaataa actagtaaac aaaaaataaa tgaaattatt ctctatcttt
     3481 taactaatta gtcgtaaata atattattct agtaaggcat tctcgtgaaa ttttagaact
     3541 gtaaatttat tggtattaat aattttcaca aacaaatcca atcttctagc tatctcctca
     3601 tatttattaa tttcattact aagttcctgt tgtaatttta aaatatatgt attgagtcga
     3661 tctaacatta ttgattccgt catgttcatt tggtcaaaat catacttagc taattttcta
     3721 ccgatctcat cttcactctt caaattgata aggacttccg ccatcttgtt taaggtggcg
     3781 attttttctt gggtttcttc tagcttcata tcaatctctg caatttctaa tacaagctca
     3841 tctttgattt cttggtattg tttgttcgtg ttagatagtt caatcataag attaatttca
     3901 gaaatcttct cttgaagact ttttaaagtt ggtcgtctat aggggagttt ttggctatcg
     3961 ttgctcaatt gtctgattaa ggtcctaccc ttgatgaaac aattattatc catgttttct
     4021 ttattataga caaagtaaga agacgtttct cgaatgtaga ctttatattt tttatgattt
     4081 tcttcttcca taatatccaa ttgatagttg ggaatgaaaa taagaccgct ctatttgaca
     4141 ccgaaagaca ccttgatata gacgccctta tcaactagct tctctatttg gttctctgca
     4201 agttccactt caaattcacg aacggtatct catttttcct taaatgtctt aaaggcttct
     4261 taaatgtctt aaaggcttcc tcaatctctt cagtggatac tttatcctta tctcgttctt
     4321 cttggaaagc atggtactgt tcctgtaaat tctctaatcc ttctgaagca atgacttcct
     4381 tatttttaaa ataatcttga aaaaatttga catcatataa tttcttatca cttatttttt
     4441 gatgacccaa acttatcttt tgattatttt ctttcaggat aaaagttaca tttttttgtt
     4501 ttaagtcaat ggttagattc aattcttttg cttttgttat taactcttct aaagaattga
     4561 cacggttcaa caaaaattct aaacgacttt caatctcttg cttagcaaaa tgcgttctaa
     4621 aaaattcttc atcatataga tctcgtttgc tgagttggcg tcctcgaata ggttttgtca
     4681 ttgctcgatc cgtcatcatg aatcgactat gcttttgact aaaatcaatc tgaacatgca
     4741 actgctttgc tttctctaaa aaatcatcaa actatttaga ttgctgcatc aaaaaataaa
     4801 gacgttgttt caattcaaat ttatgactag attccctata ttttttataa cctcgataag
     4861 aataacgttt ctcaataatt tttgcaccag ccattttaga aatacggtct gaaatcatac
     4921 ggaggtttcg ttccaaggca tagttccata tcaacttttt atctgaattg cggtcaatag
     4981 cgttgattag gatgtgatta tgaacatgat ctttgtctgt gtgagttgct acgataaact
     5041 taaaacgacc tcctgtcaat tccatcatgg tctcataacc aatgcggtta atttcttcag
     5101 gtgtcagatt atcttcagga gaaaatgatt ggatgagatg atgggcatga atagtttgtt
     5161 ggtgtttttc ttgtcggtca tttctggact cgtacaactt gtcgttatta gtaaagttaa
     5221 cattgtacat ttctaccatt tctgtatggc taggaaaatc taagtaatta ctcatgccaa
     5281 aatcagatac taattttaga ttatctgttt tatctggatt gaggatatat ttaatcaaac
     5341 gcctacgata tttttttccg tgcgtcgcaa agtgtttagt aactaccatg aaactccttc
     5401 agtcttttca cctacacttg aaattctttg tccacttcct taattaactc tccgactcct
     5461 ttactcaatt cttgtaattg gtcctgagaa atcagatggc tttgattaat cgcacgcgca
     5521 atttgattga tgttatttcc aatacgtctc aattcaaaaa ctaactggtc ataagtatca
     5581 gtgttaatcg tgacaaatga catattggga ttaagcaaaa cttttctggc atagactgaa
     5641 aaattttgac aattgctctt agcaatccgt tcattcaatt ggtgtaattc tggatcagtt
     5701 agaaatactt ttttgagatt ggtacgatag cgataaacca ttacatcctc ctaacccata
     5761 tatttttcac gaaactcacg actgagtggc tgtacttgat tcacttctgc aatcaattct
     5821 tgaacgcaag tcaataagat tgagacgtgc tcttgagtca cttgatgatt ttctcttgcg
     5881 acaaccagaa cttcatacac atctcgacta atctgttcaa acttttgaga ctgccaaagg
     5941 gagaaccaac tttcaaaaat tctatcctga taattctttt tcaataaatt ttgacgaaga
     6001 aattctgaga aactatccac tttttgttcc ctcatcattt cttttatctg tttttcttct
     6061 tgtctcgtga ttctaaattg cttacggatt aacttaattt cttgtcccat cttaccattc
     6121 tttcaacatt atacttgtct gacaatgtaa ggctgcctca cattgtcagt acgattccta
     6181 aagacctcag tctctccttg ttcggtcttt gcgagctcag atattgtgtc cacaatatcc
     6241 caaaaatcat atcgccagcc tatcaaaacc taccagtttt gacagctaac gataagataa
     6301 cttagtggct ccgcccccaa acccccaggt aaaattctta gttggatttt taaaagttga
     6361 gaaaagtata gcagaagaaa tttggggaag ttatcacaaa tcaagtaagg actaatctct
     6421 aacaccactg catgtaccgt cctatattta tgatcagctc ccatcgttta cattttcagg
     6481 tcactgtctt ttcataccag gaattagaat tgacaaattt aattagagag gtattcattt
     6541 aattgttcac ttcttcgctc catttccatt acagaaattt cttcaatact atgtgttcag
     6601 ctgactccca tgattcgttg tactacgtcc taaacgctca cggaataagc cgtacatctt
     6661 tcctagttta ctctcgtaat ttacgtatat aggttacgac acctatttgg attttagagt
     6721 tcctcatccg ctatatatgg cttcaagtca cgattctgtt tgtagaacct tgatttcgct
     6781 aattccttcc tatcccccct acctcacgat attcaggctt aggagtcgct attggtttca
     6841 tctgcagcaa atatccacag aggacttgga cctcagatgt acactatgcc tgtcggacaa
     6901 agaaaaaagt aggctaaatt tctaacctac tttttctgac tctactctga taaagccgtc
     6961 tcatatatag acattttatg cttgaaggaa tcttttcctc cctctaattg attgatgata
     7021 tgatttttta tatatttttc actaacatat ccctgtgatt caatccatcc gttaactcca
     7081 tctgactcca taataacaac ttgatctgtc atagaatttg ttacaattgt agcattatgt
     7141 gttgctaaaa taatttgacg ttgagctttc acatctctaa actgctgaac taaatgcctg
     7201 taaatataac gattgtctag attgtcttca ggctgatcaa taatcaatgg tctgaagtct
     7261 ttagaataat cactatatgc taataaaaaa tcaagcatgg ctacaacttt ttgtcccaaa
     7321 cttaatacac caacttcttt atagagaata tcaagttgtt gcgaaaattc tttcgagtta
     7381 atattaaact ccaaaatcgt gtattttctt tagaaaaaat ttttcgttta tatgcacctg
     7441 ataagtgaga accttttttc aaaatgtcat aactattgaa atgagcaata tagttaacta
     7501 ttttttgatt attgaaatcc ttcattatag tcagtgaatg ttgataactt ccatctttct
     7561 cacttataat attttcactt aaccattgat taacccatga ttcttgttca taatcatata
     7621 tacatacaat atgcaattta tctgctgcac taatttctac tccatgtaaa atatgaggat
     7681 gtatatcata aatcggataa tttttcatta tgattgagac tgccatttgt aactttttaa
     7741 tacctttggt agtattatga tcagttacta caactatttc tattccattt tttatgattg
     7801 cctctgctaa cataagaaaa ctaatatatt cttttgaact agaaaaaaca accttatcaa
     7861 aattactaaa aaaaattgtc ttatcaactt ttattttctt attttcaaaa aatatatcat
     7921 atagttcatc ttcagtaatt tttctatatt tcgttgcagt ccaattagaa aatagcttgt
     7981 aatcataaga aacaggtgta tgcaaatgaa ttaaagtctt gtgaaagcta ccgaatttct
     8041 ctttggaatt tgttagttct tgataaacag actttatctt gttattaaac cctctcattt
     8101 ctattctcct aaaaatatca attgtttatt tttcaagtaa ttcattaagt ctcttttccc
     8161 ccacctgcaa catctccttc tctacctgac tccactcccc aaagaataac tcttgaagaa
     8221 catctgctgc tgaagttgta ttttcttttg aatcatatac acaacttcta tctcgttggt
     8281 aaatttgaat tctttcaaag atagctagtt cttccaattt tcgtgtgtta tcaactagat
     8341 gatttacaat gaaatcatga tgttcttttg gagttgcgcg tgcttgattt ggattgataa
     8401 tgtacagttc ttcataacgg ataagagtac ttagatacga caattcaggc tttgtcgcaa
     8461 ttaaggccaa ttgtacttca tatcccttat ttttcaagag ttgtgccgtt ttctttggaa
     8521 catcaattgt tcgtaaagtt ccctcgatca aaagattgta tcccaaacta ctcaattttg
     8581 ttactaaaga ctctaccatt tttcctgcaa aatctttggt atattctaca ctgtctttgc
     8641 catattcttg ctgcagttct aaatagtgtg gatgctgaga acgaaaacta tcaccatcta
     8701 tgataacaat atttccttga aattctttct gtttaatacg atgaattgta gtcttaccgg
     8761 caccactttg acctccaagc aaaatcgcta taggttgctt actggacttt tttcctcttg
     8821 tcagtgaacg aagattcctt gctaaagcat gtttgaattc actatcagta taatcttgga
     8881 tttccattaa gctaccatcc gcttttcaga tatctctaac atacgttcaa ttccatccaa
     8941 atagccacta tatctctcta tttcatcaaa agtttctact aaatagatat tcgtatgaat
     9001 caaatcagaa agatcatcac tcattaaaat ccaaggatta gattcatcat caatgctaat
     9061 tccctgacta tcttgatagc gatagagtcg agataataga ttagcacctc tttctttcac
     9121 aatttcaatt tttaaagtca attcataatc ttcaacagga ttgagcattt tatcttctcc
     9181 tacgatatcg acataagata cattaaactt ctgacaaatg atgtctatta attcggtaga
     9241 gactgaactc gttccatttt cataacgact cagactattt cgtgaaatac ctacaatccg
     9301 tgcaaattcg tgttgtgtta agtcatgtgt tttacgtaag gattttatgt tctttccaat
     9361 catggcaaac tcctttattt ttataattca attatagcat aaaaaagcat aatgcaccaa
     9421 aaatggtgca ttatttcttg tttttctatc ctaaacttta cttctataaa agtcacctaa
     9481 agagccataa atatttattc ttgtattaat tttttcgatg cgcaaacgat atctacaaat
     9541 acatctataa cgattcacct tttaggattt ttattgtcaa aattgccttt atctacaaag
     9601 acaaaatgct tataaaatag tttgaaatca aggattatcg cctttttcgt tgtagcaaca
     9661 ctccacatgt gattattagg ctattagcaa agcgtatcca atacctcaat aaagtgttgt
     9721 ttttactgat attttaacta tattgtatag tctactattt aaatctcaat taggatattt
     9781 tatagaatac ccaaaattca acaataacta aaagtaagat tccattgtat ttccttgttg
     9841 gtctttcata tcacgaattt tatttttcag aatattaaag ttacaaattt catctctaat
     9901 atcgttatcc gctctgtagc cattaatcat ctcagtcaga gttttccctt tactaccatt
     9961 ctttatagta gtatttactc tttttatact gctttcaaaa ggctcatatg ttttatactt
    10021 aattacttcg tctttaaaat taaataaagg tgtatccggc ttagcattcc ataaaataat
    10081 caaccacatt atagcattat aaagctgctt tgcagtttca ttccactttt caggttataa
    10141 atttccactc cctgtattgt catattcttg tctattattt acatgagaat cacctacaag
    10201 cggattaaaa agaggtagcc ctttgtgatt aattagttct aaaaaatccc ttgccgcacc
    10261 catgcaacac tgaataactt ccttcttgcc atcaatttta ctctcagcga taaatacata
    10321 atattcatct tcaatagttg ctactgcatc actatgtttt tgttgatttg ctaacagctt
    10381 aatatgtact ttaggctgaa cttcaaaatc tctaacaatt ctttgtcttg tttcatgtcc
    10441 tctacaattc atttaagttc tcctttacaa gtttagtaac tagccttatt atactatata
    10501 aggtatgaaa tgtaccataa tcttcatcaa ctagttttta gaaaatcata aatccatctg
    10561 gagagatgac aagctgatta tccaattctt ggcaattgct tgctagtgct actgcttctt
    10621 tctctaaaag ataatcttct gttgattggt tgaatattgc tttgaggatc cgtccttcgt
    10681 atttccattc cagagctact agatcagagt tgatttcttg aaggctatac ttgccatgcg
    10741 aaatgattac tgacgcgtat ttccgaatct taatcagcct cttcataaag ttcagcatat
    10801 cattgtcact tgatacacgt tcccaaggca tacaacgacg acaatctgga tctggtcctc
    10861 cagtcaaggc tagctcggtt ccgtaataaa tgcacggtgt tcctttttgt aaaaagagaa
    10921 aggctaaggc tgatttaacc agttgaacat cttcattggc cgtccacagg attcgctctg
    10981 tatcatgtga atccaagaga ttaaacatga cctctgaaat ctgctgcttg taatacatag
    11041 actctccatt gatttcatcg atgaactggt ctgtcttctt aattcctcgt aagaaatagt
    11101 ccttgatact atcagataaa ggataattca tgacggcatg gaactcatct ccatttagcc
    11161 aaggctgaga tgtatgccag acttctccta ggatataaag atcaggattt ttagctaaaa
    11221 ctgccttacg aaaatccttc cagaactgat ggtcaatctc attagccaca tccaaacgcc
    11281 aagcatcgat attaaactct tcaatccaat aagtcgcaac ctttaaaaga taattcttga
    11341 cctctggatt ggctgtattt agcttaggca tatagtcctc gaaaccaaaa acatgatagg
    11401 gtaagtctct cttattaact agcttttcag ttgtcactgg gaattgttga atatggaacc
    11461 aatccttata agcagactgt tcaccatttt tgacgacatt tttccattga agagattgcg
    11521 aagcaatatg attaaatacc gcatccagca tgactttcat gccacgatga tgcgcttgat
    11581 ccaccagttc ccgaaaggtc tccttgtctc caaaatgacg gtcaatttca aagtaatctg
    11641 tcgtattgta cttgtgattg cttgtagatt caaagatggg acaaagatat agtccagtaa
    11701 tacccaagtc ttgcaagtaa tccatatgat caataatccc ctgtaaatca ccaccaaaga
    11761 aatcatcgct cttaggtgtg acagatgaat cccagtctaa agtcccttct gggtttaata
    11821 gagcattgcc attggcaaat ctttcaggaa atatctgata ccataccgta tttgaaaccc
    11881 agtcaggaac cttgcaggca tcaatctcat gaagataagg caacttaaat ccattcccaa
    11941 ttgcatgaag attttctaga gaattttcca cacacccttt atcgccatac aaaatatttt
    12001 gaccttctgt atctctgagc tcaaagagat actggatacg tgcaaagtca actgacactt
    12061 caacctgcca atggtcaaat aaggtaccag aagttatctt gaccatttct tttgtatcct
    12121 gataaaactc ctccataaag ataaaagggt ccccatagtg caagttgatg ctttcaatgt
    12181 cccctttctt agttcgaatt cgaatatgga gtttcttatc cttataaaga taggcatact
    12241 ccgactcagg cccatggtaa atagcactca attccatcaa tttgtctcct aaagtattca
    12301 ttcaaatttt acgcaaacgt tttcaaactc aataaatcca gtatactatt tttgtaaact
    12361 atttgcaagg aatgtccgtt gttaaaggaa ctatctttta aagtatacaa aaagccttaa
    12421 aaaaggcttt ctctatatac taatcctctt tcggttttga aaatacttct actcgctgag
    12481 ctaggacttt gatttctaca ggtaggttat ctgatttatc tccgtcaaca tctgactcca
    12541 attcactatc tgaagagatt ttaatattac gcgctttgat atactcgata ttatcatttg
    12601 cgacaacatc cccttttaat aaatcaggaa tgacggataa tttggagaat atagaggcat
    12661 ctttcagaat caaaatgttg gcatagccgt ccttgttttc ttcaaagatt ttcttatcag
    12721 cgaagtaatt tgtcaaaagg accaaaacat ggctagcttc accaacataa tttccatttt
    12781 ctgtctcaac cttaatgtta aagacctgat ctgtcatgac agacttcatg gtatttacag
    12841 cataggttaa aataccgaat tttgttttgt cctctatttc aacattgtga atcgcctcag
    12901 gcagagaacc gatactaaag atataaccaa aatagttgtc atttgcttta ccgatatcaa
    12961 tcttattggt taaatcaaaa tctagttcgt caattgcgcc atcgatgtct tgattgattt
    13021 ccaaaagttt tgtaatgagg ttacccgtac cgcctgggat aatccctaac ttaggaatgt
    13081 agtctctctc atcaatacct gaaatgactt cattgacagt tccatctcca ccaaacacaa
    13141 ccactgcatc atactgctca cgagaagctt cttcagcaaa atgtgttgca tccagcactt
    13201 tttcggtaat tttggtttca acatattcaa agtattcttt tgctttattc tccagctttt
    13261 ctttgtaatc caaagccttc tcgccaccag aagtagggtt gataattacc attgcttttt
    13321 tcattgattt tatccttaat tttaaacaga aatgtttaca tttcgtcgca tgcaagtaaa
    13381 tgtaatccta ttatacaatg aaaatacaga aaagagaaat ctgacgtact ggagattaat
    13441 acgcttttat tctattttcc catcgcctaa ctacatcctt taagggttca tccaagtaag
    13501 aataggcctt atccttgatc caatcaggaa taccgtaagc tgcctctgct atgctacaag
    13561 tgattgctgc gagagtatca ctgtcgccac caagtgagat ggcatttctt atcgcatctt
    13621 cgaagtctct actttcaaga aaggcgataa tggcttgagg gacagtttcc tgacatgttt
    13681 cgttaaaacg atagttagga cggatttcat ctaaagtttg agatagattg taatcgtatt
    13741 ctttttctat ataatcctta attcgtcttt tacactctgt aggattgtcg ttaattgatt
    13801 gctcgtactc tctacaataa ccaccaaagt aaaaacgaca tagaaagata gcatcagctg
    13861 tagccatagc gcctttgaca ccttctggat gattatgagt tacctctgca gaaagactcg
    13921 taagtcctct agatgatggc cacataccag ttttcgcata aaaaccacag tccatgatcc
    13981 aagcacatgg agaaatacgc atagctgatc cattcccaaa gctattataa ggctcacggt
    14041 tatcgctgtt tagccatgca ttaaaccgag caccgtaatc agcattcgga tacattctgc
    14101 catatttctt catcgcgtca atgaaatcgt ctttttgtcc accattcata attgcttctg
    14161 caacagcaca ggtcataacc gtgtcatctg taaaaaagca gtccttccga aataaaggaa
    14221 agtcctttgt tttgatattg ttccattcgt aaacagaacc gacaatatct ccaataattg
    14281 ctccaagcat cagattcctc cttgttcatt ttgatgcttt ttatattggt tatctaccat
    14341 atttatttta gaaaataaca tcctgttgga ttttaaaaat ttcatttttt caaaataggg
    14401 ttttaccatt tctttccacc tagctctatg aaaattgatt gattttaaag gagataggcc
    14461 ataatttccc aatgcataac catcatttac ttcaacaaca agtgttctgc catcgcgagt
    14521 aacaccgata tctagtccat aagctattgg cgcatctttc caacatgata tcgcttcatc
    14581 aattacactt gcatcaaatt gtgcatgata atcacctgta tagggtcgaa catctaatac
    14641 gcgaccatct aacacaaaac aacgccattc agctatgaat tctacaacct cactaatcca
    14701 tataggatag tcgaaaggta gaccaatacc tattaaatca tgggtttcat taacaactct
    14761 tccagtaaag acttttgaac cagctttagg cttaataaat tttccccaat tatcaggtat
    14821 attcacaatc tctcctaaaa taccagcata aatctttcga ccataaaact ctttaagctc
    14881 aataggatag tcatgaaccg gaacgtttaa gcccatcatt tttagtaatg ctctagtctc
    14941 cattatataa tctacaacta tatcttcact tgttaactct tttatttcag aaaaagattg
    15001 atataaaata acttcttctc cttgtaagta ggcacctact tgagcattgt atttattaat
    15061 tgaaacctca cttggtaatt tactttgtct aatataaaca accatttcat cactcctata
    15121 tcactagtgt tacaccaatt tgtaaaaaat aatagcaatt ttgctcttat ttttttgagt
    15181 aaatagcccc cataatatca tcgaaataat caacggtatt taggagtaat tcaataacct
    15241 gggactttgt tagtcgcatt ccccttctat ctctagcatc ttctactaaa ttttcaagtt
    15301 tctctagatt tttatcatcc aagctaatca ttattctatt tttatcggtt gccattttca
    15361 tcacctcaag ttaattctat cacaggtgta acacttgtgt caactggctt ttataataca
    15421 ttagtttaaa agtggagagg atttttaaca cagtaacttt aaatctttgg tattaaaaaa
    15481 ttttcacaat atttatagaa ataaaatctg tctcaaatca gttatcaaat ctagtataaa
    15541 ttatgagcgg ctactctaat actttccctc taaacaagaa aaagacttac actcaagggt
    15601 tttcttcccc cccttcgtta taacgttttg actcttttac tagcaaaggt atatactcac
    15661 aaggaacttt ggttgactat tgaatctctc caacttcttc tttaacatat ccttctacat
    15721 cttcaatctc tacaaacatt gggtctaagt gacacaagaa atgccaaact tcgatccctt
    15781 tttttctgta aagaatcgct tcaccgtctt cacttccgaa aaagcttctg tcgatttcat
    15841 atccgcggct ttctaagaag tcttttgctt tacgatagtt cgtttctctt gtttcgacat
    15901 aggctttaac ttcatggttg ttaacgacat atgcatcaat ttttgaatat ccttcgatca
    15961 ctctatcatt tttgagggat aaatttgaaa tctctttcca aataatgttt acattttccc
    16021 caggatcgaa cataaatcta gataaaggaa caatatttcc gttaaaaata atttccatat
    16081 aatccggtat gtttttagga ttaaaatact ccacttcaac accatcttct gtttccagag
    16141 tgtatcccgg gatttgagct acaaaggctt tcccatcttc tatggaatca aatgctacta
    16201 aatctttaga ataatcattt tggtacaatt ccaatataac catcgataat ctctccattt
    16261 tcattatcag gctaatgtaa ataagcacgt cacctgacca attcaggctc tctgtatcat
    16321 ctcatcatat ttcctactta ctttacgagt cctataccca gaacacacct tatcgacctt
    16381 cggtctcacc tcgtcgcatt ggctgaacat ctacttttac tttgctgatg cttcaactcg
    16441 tacaaacagc gataccgcct cagcgtgatg cgtcagtggg actcaaaagg ttcggggaac
    16501 cttttgagga ttaactacgt ttctctaata aacttacaca ttcaacttgt tcatcattgt
    16561 ccaaacctat gttgagattt tcttctataa ttggtagctt aaaagtaatg gattttagcc
    16621 attgtccgtt agattgtttt tcttcataaa cttgaatttc agaaatcaaa gctgaaatta
    16681 actgcctacg ctctacatca ttcatgactt tatagagctt atcaaaatag atcagaacct
    16741 tatatatgtt atctcctgta agcttttcag cttcaatagt ctgtttcttt gctttcgcat
    16801 caattagtga tgattctaat tcatctattt tgtcatacat acgatatagt ctatcatcta
    16861 aatcctgttt ccttctctta taatgcttat cttcaacatc taaattatct atttcctcaa
    16921 ttagcttaaa ctttgtagaa tgactctttc tcaattcctt ttggtaatta tctatttctt
    16981 tttctatttc agaggtatcc accttcatgt tgattttttc ttgcatcata gaagcaaatt
    17041 tcggattact tactatcttg acaatcacct ctgcaacagc atcatctaac aattcttctc
    17101 taatttgctt actgaatgta cacttatgac ctcttatcat ctgcctatgt ttacaaccat
    17161 agtaataaaa atctttatac tttgtgccat ctttcttttt cttgatacac ttgttcccaa
    17221 acattcccac tccacatatc gggcatttta caattccaga aagcaagtgt gtgcgtgtat
    17281 cttttccttt attcacatgc tcatatttct ttgcttgaga ttttagctta acctgagcag
    17341 cttaccaaac ttcatcggaa actatagctt catgtatccc ttcagatatt agatattcat
    17401 cttgttcaac ctgcttatat tcatttcttg taccatgaac tttttctaaa gttcttcttc
    17461 caaatgctat tttcccatta tatacaggat tctttaatat ctttcttata agacctgcat
    17521 caaacaaagg attcttacca ttctgtcttg ggatttttct aattccatga ttctctaagt
    17581 atttagatat cccattggct cctatcgtag tatttacata ctggtcgaaa atcgttctta
    17641 ttgcaactgc ctcttcctca tttataaaca gcttgccgtc ttcaagttta tatccatacg
    17701 gagcaaagcc accattccat tttccttccc ttgctttttg aatgcaacct tccattgttt
    17761 gaatacggat gttttctctt tctatttcag ccacagctga taaaacagaa atcattagtt
    17821 tcccagcatc tttagatgaa tcaatgccat cttcaacgca gataagatta actccataat
    17881 cctgcattat ctgaagtgta gaaagaacat cagcggcatt tcttgcaaat cttgataact
    17941 taaacacaag aacaaaagat actccatctt ttccagattt tatatcttcc atcattcgat
    18001 tgaactgtat tctaccttca atagacttgc cagacttccc ggcatcttca tactctccaa
    18061 caatttcata atcgttgtaa atagcaaaag ctttcattcg tgatttttgt gcccctaacg
    18121 aatacccctc tatctgtatt gacgtagata ctcgtgtata gaggtatact tttatttttt
    18181 cttttgacat agtattaacc tcaatataat ttttctatat catatataat ttttttaatt
    18241 taagtttgga ctatcatttc aagtatatta taacactttt attagtccgt ctcaatttgt
    18301 gtttttgcca tgtcaaaact atttttcatc tcttgatttt ttgctggcgt tggatcgggt
    18361 agattatcta aatctaaagc accagcatat tttgcaatca gatttgctat taaatcagcc
    18421 aatccattcc agtcattgtt caatatatac ctcctctaaa gttttatatc taataattat
    18481 ttgtttaatt aagttttttg acattgacaa gtgctttgga ttagcaacat aggaatctca
    18541 cctccgcctc tattccggat gagccggctt caaccttaga agtatcatta tcctcatttt
    18601 cttcatagcg gatagggtat ccctccctat attcaaactc ttacttatcg ctcactttct
    18661 ttttgtttag cagaactttt tttgccgaat tattcagccg aaagatcttg acggataggt
    18721 tattacgctc caaaaataat taacgtcttg tcttggtcta ttcaattgtt aaggttcaaa
    18781 atttatcgaa agttattaat ctttttaaaa tttgaccatc agaaaatatt tatcttgatg
    18841 taacaaaatt ctataaatta ccctcttata cttaacagtg aaaagaagtc tttcttggta
    18901 accaattttg aaatagaatt tgcttatata aaaaggtcca attcccactg cataagtagc
    18961 agtgaaaatt agaccctctt ggtaactgtc atctaaaagt cttctagcat ttctttaatt
    19021 ttctaatcgc ctttaaagtg tcatatttat atttttttat ctctggcata ctttgatacc
    19081 tttttagact taaagtcttt aatagtgcct ttccacctct ttttatctat aaagattctc
    19141 ctacatcata attcattttt ttatttaaac ctttctgtct tagtttgtct ttatcttctt
    19201 cataccattt taagattgtc acatagtggt tttgataggt cttaccactg ctttccatgt
    19261 atctggatag tttatttatc attatatctg tgtgtgagtt taatttttct tttagatttt
    19321 tatattcttc tttgcttaac cttacatttt tgaattctcc ataaaaaatg ggggtggact
    19381 ttttatctat ctctccctct ctctctttat ctatctctat atctttccat gtaattccaa
    19441 tctggagtac ctctactgtc tatcggtaat ttaattttga tatctggcaa tactgtgcta
    19501 gatatttgat ctttatattc agtatttttt aaagcttgcc taataattga agttaaatag
    19561 aatgctactt ctttattcaa ttctttattt tttaatttta aacaatgaat tttcatatct
    19621 aggcttgctt tatatttatg ataaaagact gctcctaaaa atgaaacaga tataaaattt
    19681 tcaaaaactc tataattttt atcatctata tcttcgtagt aacctaagat accattgtca
    19741 atatttgtag cactaattct aggagttttt ccatcgagta aatatctttt tggaatagat
    19801 gagcctgttg gtacttaact cgatttcccc tttttttcgg taataaatat ttctttttat
    19861 tttgttgtct gatatttttc ctacctgtcc tttgtaggat gagtattttc tagattttct
    19921 tgaataactt tttacttgaa gttttagctt ttgaactagt cgttgtactt tctttttgtt
    19981 tattatcagt cctgatcttt ttaatattgc tgttattctc tatatcctat ttttcattca
    20041 tgatattctt ttactaattt tatcttaaat tctgtgctgt atttgccatt aaaaaactga
    20101 cctcctttag ttagtttttt ggcctaactt ttgagggtca gttcaaaatt tgcgactttt
    20161 aaatgaattc caatattcaa ttattaagag ttaacatggt gcttgccaat aggaatcatt
    20221 agaggcgaat tggaaatagg gtcacgtata atttttgctt caagattaaa gatatcttta
    20281 actagtttat catttagtat atcttcaggc tttccctctg caacaagttt accttcttta
    20341 attgcaaata ggtaatcagc gtatcttgct gttagattta tatcgtgcaa aatcatgcaa
    20401 atggttgtct tatatttttg gtttagatca gtcaagaggt ctaatagttc tatttgatat
    20461 gagatatcca agtaagtagt tggctcatct aaaagtagga tacttgtatc ttgggctagg
    20521 gctagagcta tccatactct ttgcctttga cccccagaaa gttcttcaac taggttattt
    20581 gctagatctt caacattggc cttaaccatt gatctgttta ttatttcaag gtcatctttt
    20641 ccaagactct taaaaggctt tctgtagggg aaacgaccac ggcttacaag atcagctact
    20701 gttattgatt cagggattat tggagattga ggtaatatag ctatgtgttt tgctaaatct
    20761 ttttctttat aagaattaat tgatttatta tcaagcaata cttctccctc taatggcttt
    20821 ataagtcgag acaaggtttt aatgagtgtt gatttcccac aaccatttga cccaataata
    20881 actgatattt tttcttcagg tatttttata tttatatttt ccaagattat tttttcatca
    20941 taaccgcagg taagattatt tgaccacaga cctttcatta tatattcctc ctgttcattt
    21001 ttattagtaa gtatattaag tatggtgaac ctaacaagcc agttacaaca cctactggat
    21061 atctagctgg taaaatattt tgagagaata tgtctgataa caaaactagt aaaattccaa
    21121 ccaatccagc taatattggg cttcttttct tgccaatatt taaggctatg ggaccagcta
    21181 aaaaagatat acaagctatt ggtcctgtaa ttgaagtaga aaaagcagtt aaagatacag
    21241 cgcaaaaaat taaaacaagc cttgaaagct cgggatttgc tccaagtccg attgctattt
    21301 cttcaccaag ttcaataatt tctagtcttt tattaaaaaa taaaactaat atagtagcaa
    21361 taatacttac tattagaaca agaggtatgt catctaactt tgtaaaagat aaagagccac
    21421 tgagccatct cataacttct tgtaattcat atcttgctac tttcaacaat aaaaatgagg
    21481 tgcctgctct tgtgacagct tgaaaaccaa tacctaatat tatcagtctt gctgctgaaa
    21541 aaccatcttt tttagctagt aaaaataata ttaaagatga tgttagtcca caagttattg
    21601 aaataattcc agtagttaaa ctatttgttt ttaataccaa tatgcaaaag accgctgcaa
    21661 tagatgaaga acttgtgaca ccgattatat caggacttgc aagaggattt cttaacatag
    21721 tttgaaagat aaatcctgcc aatccaaaag accagccagc tataattcct gctaataatt
    21781 ttggtaatct aatttccata atcgaaaaac tagctccagg aacagtttca ctatttaaga
    21841 ctttaatcaa agttgaaaaa gaataacttt catctccgat aagtaaaatg aaaaatgata
    21901 gactgattat tattaataaa aatagtgagg aaaatagtgt tattctattt tttctttttt
    21961 gaatacctat aattaaattt tgcattagtt attaacccct ctatttttca tagttacata
    22021 aataagtact ggacccccga ttattgcagt aattatccct acttcaattt cacctggttt
    22081 acctaacata cggccgatta tatcacatat aagcaagagc tctgcaccta taaaagatga
    22141 agaaatggtc attgtgcgta tatctttgct tataaataag ccacaaaagt gaggaactat
    22201 aagacctacg aagccaatag gtccaccaat tgcagtaata cttgaacata aaagcacact
    22261 tgcaattatt gcaagtgatc ttatcctatt aacattaact ccaagaccaa cagccatttc
    22321 atcacccata gctaaagcgt ttaaatctga tgaaataaat atagctatca agtgacctaa
    22381 aattataaaa ggtagtagtg tagatataga agataatgta gctgctccaa ggctacctat
    22441 ttgccaaaat ctaaatttgt ctaagacgtt attattcggt aaaattaaaa aacttacaaa
    22501 actgcttaaa gccatactaa cacaagttcc tgataaggca agttttatag gggtaaggcc
    22561 tgcttttccg cttacagcaa tcgcgtatac aaaaattgca cttactaagc caccaatgat
    22621 tgcaaaactt atatggctta tgcttgatga aattcctaaa aaagaaagac caattactac
    22681 actaagactt gctcctgtgt ttataccgag tatacctgga tcagctattg ggtttctagt
    22741 aactgattgc atcaatacac cgcttatggc aagactagaa cctgctaaaa ttgcaaaaat
    22801 agttctaggt attcttttat atataattga tttaataaaa tcatcatttg tattgcccat
    22861 tccaaaagct gctaaaaaat ctctgatatt aatttctttt gttccaagct ttaatgacaa
    22921 aaatattgta ataagaagac ctaggcagat tacaaaaatt atgcctaggt ttatttgttt
    22981 tttatttcgc atttttgcat gcatttccta aaagatttag gtattcttca atagtatagt
    23041 ttattgaaag tggtgttgga gtgcatgagg ctgctaacgg tgtattatct ggaattacag
    23101 caacggcacc atttttaatt gcatttattt tacctaaaag aggatctttt tgtaaagctt
    23161 caagagtttt atcatcacca taagttatga ttacatcagc atcatttatc ttatttgctt
    23221 cttctgcaga aatttccttt gcaaaactat cttcactctc aaattctttt aatgattcag
    23281 ggaaaactag acctaaatct gttaaataat ttgctcttgg atctttgcta gtataaatcc
    23341 agaattttga tgtatctgca gcattaatca tagtaaataa tacttttttt cctttgattt
    23401 ttcctttgat ttctggatgt ttttctaatt ctttggatat acgagcttca gtatttttga
    23461 ttaactcatc accttctttt tccataccta aggcttttga atcaatttta atcatatctc
    23521 tccataaagt ttgccaaggt ttagatttgt atgctgctac aggagcaatt tttgatagag
    23581 tgtcataatc ttctttagtt ataccagaat aacctgctaa gataacatct ggtttagaat
    23641 ttgatattgc ttcaaagtta agtccatcca aatcgtcaaa taggttagct ttaccattta
    23701 gttctttgat tttttcttct gtccatggta aaactccttt atcagcactt acaccgtaat
    23761 ttgcttttga aaatccaaca ggaactattc ctaaagctaa tgctacatca tgatttcccc
    23821 aagcaatagt tgcaactctt tcaggttttt tatctaatat agtttgaccg aaagcatgat
    23881 ctaaaactat tttatcaggc gcatgctctt tagaagtatt ttcttcattt ttaacagaat
    23941 tagaagaaca agcgctaaaa actatacaca tagctaaaat agctattaga aaaaatttgt
    24001 ttttcataaa aactccttaa acatatttca agtctattgt atcgataata attatcattg
    24061 tcaatagata cagttacaaa tttaataata taattttttc aaaaaaggat ctagttttta
    24121 tttttagtta aattatgtct atatttttca ttaactcatc aataaaaatt tgtttgctaa
    24181 tatctctttc ttctgatttt gtttcaagga ttattgctac atcattcata tttttaggta
    24241 aataccaacc atctggctta tctgaatatc ccttgaagcc taattgatta aaagtagtta
    24301 tttgaccagt accttgattg atgttttctt caacttcatt aaagcctata cttctcttgc
    24361 ataatcccta acttgatctt ctgtccttaa agtcattttc tattcctcgt cactaaaatt
    24421 atcttcaaaa ctaataatat cattaggagt gcaagataaa gccttacata ttctttctaa
    24481 attcttaaat gtaattggtt tatcctttcc catttgtgcc ataacatttg ttgtaagtcc
    24541 tgccatagct attacatctg ttttctttag tccttttttt gctaactgta tccataatgg
    24601 tttatagtta agtgccataa tatccctctt tttttcttca ttagatttat ttaattatag
    24661 cataaatatt gatttcattc aatcttacat tgatttagtg atttgtttta gagaatttgt
    24721 tgtgttattc cgtccttatt ttgccataaa ttgttttaag tgggacctct tatccttacc
    24781 atttttacct cctaattttg agtataaaaa agacgataga gtttttaatt tctatcgtct
    24841 gagatttaca tattaaattt tcatcacaaa ataagactct ttaaagaatc tttaaattca
    24901 ttggtatgct gcttacaata tccattatta aaatggatat tgtaagcagc acaccaacta
    24961 tttatttttt gagttttaaa ctttggtaga agtcttgaac tccttatatt tacataacta
    25021 tttgcctcta taactgttat gacacgaaac caacaagcaa gttcaagcac ctccatatct
    25081 ttattcaaat gaggactaag atattcacaa actttatcat aaacctcttt atcttttttc
    25141 ataaaagggg catacaactt agcaaattta tcctaaaatt ttttgttgcc tttatcaatc
    25201 atttcaacgc tcctttaaat ataaaaatga tcctatcaat ctttatattg agcctatttt
    25261 ctaattgata ccttgcaaga aaggaataat agctgttcct ataccaagta ggaatgtaat
    25321 aaatgcagaa agtcatatac tgtcattctt ttctattaaa tcattttgaa gtgtctcaaa
    25381 gcatccatga tggcttcttc tttttcaggt gtacactgtg gaataatttg tttatccttt
    25441 ttagacttgt tgtaatgttc tcgtaattct attccacatt tcttttttat ctgtgcaata
    25501 tataatgtcg aaacttttaa ttcaaattta ttccaaacat attctttgat ttgagcatat
    25561 gttgctttgc tctccgcact tgtcaaatcc atctcatcca gctcaatttc aacacttatg
    25621 tgcttatcga catcgagttt ggacaaaagt gctaccgtct cgacgtgatg cgtttgagga
    25681 aatagatcca ccggctggac tttcttcaat tcatatccca actcttggta tagtttaata
    25741 tcacgcgcca tggttgcgac attgcaggag atataggcga tgcgatcggc tcctgtttgg
    25801 gcgcttgctt tgataaagct ttctgtcaag cccttgcgtg gaggatcaac caagataacg
    25861 gttggttgaa taccttcctt gagccatttc ttcatggcat tttcagccgt gtcacagaca
    25921 tagtgggcat tagtaatctt gttcaaagaa gcattcttct ggctattctc tactgcttct
    25981 ggaatcagtt caacaccgta gacttctttg acatgcttgg cgactgataa accaatggtt
    26041 ccaataccag aataggcatc aataatcaca tcatcttttt ttaactctgc aaagtcaatg
    26101 gctgtttgat agagtttctc cgccatttca gtattgactt ggtaaaaggc tgggccagcg
    26161 atttggaagt catttcccaa catctggtcc gtaatatagt cttgaccata aagagtgcgc
    26221 cactccttac caaaaatcgc attggtattc tggtcgttga tattttgcat gacagacaca
    26281 atctctggga actgcttgat aacttgttca atcaattggt caacacgaaa aacttttgga
    26341 cgagttgtca ccaaaacgac catgatttgt cctgaatagt gaccacgacg caccacaaga
    26401 ttccgaatca atccagattg ttccttttcg tcataaggtt ttaaatcaaa acgacggagc
    26461 aggtctcgaa gagctactac gacttggtca atgacaggat cctggataaa gaaatcttca
    26521 aggggcatga ggttatgcga attcttacgg aaaaatcctg tttccaagac accattcact
    26581 cgacgaacgg gcacctgcgc cttattgcga tacttgactg gatgttccat atcaagcgtt
    26641 tcagcaactt ctacatctgc aattccagca atcttgtaga gactgtcctt gacttgcttg
    26701 gttttaaact tgagctgttc tggataagaa aggtgtccta aatccgcgat tcctgaacgc
    26761 aggtaagcca aatctagatc ttgattacgg tgtggtgact ggacaaggta tttttcaact
    26821 tttccaaagc caatcttttt attgaccttg aggacacgca tgagaatttt ttcactcggt
    26881 aaagcattct ctacaaaaaa gaccaaacca tctaccttgg caactcctgc cccttcatgg
    26941 gtcaaatcaa caatttcaac ttctacaata tcatttttct ttaacattct tttctctttc
    27001 tattggccga caaaaaagac ggcaacgtta cggttaccat ccattatacc atgaaatttc
    27061 ttaagaacca aaactcttga agaagttgac aagggcttgc cagagggagc ttaagaagtt
    27121 accaccgtcc tctagcgcct tatccgcatc aaagttaagg ttgatatttt taaaactgtc
    27181 gccagcttgt gatacgatgc tttgtttaag gtcatttagg gttttagtga aatctgcatt
    27241 gctgaggata tcactctttg agagattcaa ggcaaaattg atgatgatat tgatctggtt
    27301 tcctgttatg acctgatcaa gtttgtaatt ttttaaggta tcttcaacaa tcttgcgtac
    27361 atcttcttct gtcagatttc ccttgctttc tttagctttg gcgagtcctg acttgatatc
    27421 agctagggca acgtttaatt tattagcatc atagcctgat ttgtccttgt tttcagcatt
    27481 gatatctgac aaagccttta gctcttcttg agccaaatct ttattggctt gcggcacctt
    27541 ggctccatta gcttctagcg aatagtagat cccagccaaa gcactctcac ctgtaactgg
    27601 aataggggct gctacagtga ttttggcatg ttccataccc agcgttactg ctgcgtttcg
    27661 gtacatatcc tgagtcacct tagtgatatt ttctggtgtt tcaatcttga cctcaagtgg
    27721 cgatttgtca cctagctttt gaatcttggc tgatgaatac aactgtaagc tagagtcatt
    27781 ggccacattc atgattttag aataaacatc aggtgtcatg gtcttgagtt ctttggtatc
    27841 tgttgaggca ttgtagccca gttttttaag agtttgattt ttttggtctt cagataggga
    27901 ggaacctagg acatattcag gttggacata ggtttcatcg ataacttttt gaacatctgt
    27961 tgctgcatgg acgctattca tagctgttac tgcccacaag accgcagcac tagtcagaaa
    28021 gagtttcttt ctcataggga atttcctcct ttacctttct agagtaatat atctatctta
    28081 aagaaaactt ataacaaaaa cacctgaact agccagatgt tgaaaagaga gtgaaacatt
    28141 tgatgatgta aaggttgagt tgcacctgtc tagaataata atagtttcct ccatttacat
    28201 agagttcggc accgtgaaaa atggaaatgg gatgaatata actataagtc tttccagtcg
    28261 tattaccaag caagggggca acagtctcac gagagtactg tttggctaga gccaaggtat
    28321 tttccttgcc attttgggcg ataaaatcga tataggcagg tccaaaatta taggcttgaa
    28381 cagctgtcca gatatctaca cccttcttct gcgccagata gagattgcct gtcagagttt
    28441 gaatgccttg ccgaatgcta gaggcattat cattgatggt gttggtggaa ccacttgcag
    28501 actcactaga ctgcataaca tcgccttctt ttccttttgt ttcagtataa atcatagcaa
    28561 gcacaagctc ttcgtttgct ggggtgtctt tttcactcaa tatttctcgc accatgggtt
    28621 gataggtcat gacttgtttg acatcttgat gaacgcggta agctttatag ccagcaaaaa
    28681 ggaagactgc tagtacaagc actcttcgaa ttcgtttaaa cattatttac tttggatatc
    28741 ctcgatattt ttgattaaga tagagtaggt tccattttcg ttttggataa actcaacaga
    28801 ctcggcgtct tgatagacgt tattgggaac gatgagctca attccatttg ataaggagag
    28861 tttttggttt tcaaatttct ttaattggcg actgacatca atttcatcaa attgaacagg
    28921 ttctggtacg gcttctttga cttggtcaat aaagctcaaa cgagccgtca gattgttgtc
    28981 aaaaaggtca ttagccaatt tctcaggtga caattcattg ctttcttcta ggttgttgaa
    29041 aatagctgat ttgaccttgg attgaaattg aaaatcatct gtgttaaaag attcagcaat
    29101 tctctgggct gttttttcca gttccttgat agatttttta ggagaaatct taggagcgac
    29161 agcaagaaga ttatctgaaa aatagttcaa aaaagtcccg ttgtacttga ttcgtttttc
    29221 aatcaggtga tacttgctac tctgaagatt gaccaccaag gcctcatcag ctcctgttcc
    29281 aaatccaggc aggttattct gagttagctt gattggatta tcaacttctc ctccgaggtg
    29341 ggtcaaggtc tcccgcaggg caattcgcaa gaaagcgaaa tgttctacac cttctttaga
    29401 aaattgaaca aaaatcaagt cattggtctt gagattttca gaaatgctaa actcctcttt
    29461 ccagagatta gccagcgtta ctgatgtctc caacaaatcg tctgtaatat gattgaagaa
    29521 gggattttct tcttcgaaaa tcccagtctt ggcttcatct gaatacacat gttcaatttt
    29581 tttacgcagg tattcttcga tttttggagt aatattgaga aacttatctg ctaagaacag
    29641 ttcggtatca tccggactga actggtgaat aatggctttc ttaatataaa tgtccataaa
    29701 agttttagtc ctcgtatagt aggaaggcat ctgtcaattc tttgactgca cttctcactt
    29761 cttctaatac agcctcattt tctgaattct taagggtttt aatgatgagt tcagccactt
    29821 tgcgactttc ttcttcacca aatccacgtg cagtaatggc tgctgctccg atacgaatcc
    29881 cacttgtctt gaatggtgac aagctttcgt aagggattga gtttttattt aaggtaatat
    29941 tgacttcatc caacaagttt tgagcaactt tgccgttttc tacaacttta gtcacatcaa
    30001 caaggaagag atggttttca gttccacctg aaataatacg gaaatcaggg tcttgcaaga
    30061 agacatctgc catagccttg ctgttcttaa ttacattggc agcatattcc ttgaaggctg
    30121 gatccaaaac ttctttgaag gaaactgcct tagccgccac aacatgctct aaaggaccgc
    30181 cctgaatacc tgggaaaata gctgaattga tttttttagc aagttcttcg tcattggtca
    30241 aaatcaaacc accacgaggt ccacgaaggg ttttgtgggt cgttgttgtt gtgatatgag
    30301 cgtatggaac tgggcttgga tgaaggccag ccgcaaccaa accagcgata tgggccatgt
    30361 ccaccatgag cttcgcaccg acagcatctg cgatttcacg gaattttgaa aaatcgataa
    30421 tttgagaata tgctgaagca ccagctacaa tcagttttgg ttttacttct tgggcttgtt
    30481 tcaagatagc atcaaagtct aagagttccg tttcaggatc aacactataa gaaacaaagt
    30541 tgtaggtttg accagagaag ctaacaggag ccccatgagt caaatgacca cctgatgcca
    30601 aatccattcc cataaccgta tcacctggct caatcaagga catgtaagcc gcacagttag
    30661 cttggcttcc tgaatgtggt tggacattgg caaatttagc accgaaaatt tcttttgcgc
    30721 gttcaatagc aagagtctct acaacgtcta ctacatcagt tccaccataa taacggcgtc
    30781 ctgggtaacc ctcggcatat ttatttgtca agatagaccc ttgagctgcc ataacagcct
    30841 tggaaactac gttttccgaa gcaattaact cgatattatt ttgttggcgt tcttcttctt
    30901 tggcaatagc attccagaga tcagcatcat atgctttaaa atcatctttg tcaaaaatca
    30961 taggtcttct cctttattgt gtgactagtc cattagtttg attttacaat aagaaaatca
    31021 aactaaaaga tgcgaataaa ccgtttctgc attttatcac aagtatagcc aactttttca
    31081 taaaatgcat gagcacccag acgatgattg gcagaattta agcggataaa cccataacca
    31141 catctttttg cttcttcttc caacccttgt agtaaacttt taccaatacc ttgaccttgc
    31201 gcttgaggtg aaactgctaa agctaagata ttaaatcctg ctttggaata gagtgattcg
    31261 taaacttcag cgtggacata tccaagtaag acatgattag ctgcatcctc atagccaagt
    31321 aggaaatgat gggaatcctg agacagtcta gctagttggc tagccgtttc ctctggacta
    31381 aaagtataac ccaaagcctc ttggttgatg tcacatatcg ctttcacatc tgtttcttgc
    31441 aaatctctta gcatttcaag cctcctcaaa agaaatctct ggcaaccgag caagaatatc
    31501 ttctcgttta attgcgcctt ggcgtaagat tttcaccttg tctccagaca aatccacaat
    31561 agttgaatcc tgtccagtta gaaaagcatc gtcttccaga cccagaacct cttggtcaaa
    31621 atccttcaga atttgttcaa aggttacacc acttgcctga cctgagatat tggcagacgg
    31681 cccaatcaag ggacctgtct ctcgaattaa atccagtgtg atagggtgac tgggcatccg
    31741 aaatccaata gttgcaaggt cagaatttac ccaataggga actcggtcat tggcttcgag
    31801 aataatggtc aagggacctg gcaaaaaggt ctctacaagt ttttgtagat aagttggctg
    31861 attcttagaa aagtgcaaga tatcctcgaa agaggcgata ttgagattga gtgccttatc
    31921 tctaggacga cgtttgagtt ggtaaacatg gtcaactgct ttttcatcta aggccttggc
    31981 aaaaagacca taaacagtct ctgtaggtag aacgacagct ccaccatttt ccaactcttg
    32041 tctaatcctg tccatcatca accacaacca tcctattttg accaaattgg tctttgagtg
    32101 ttcgaacttt tttttcagga agatgttttc taaaaagttc gggaacactt tgaccttgct
    32161 tgtatccaat ttcaaggtaa atcttaccac catccttgag atagtctttt gcatcttccg
    32221 caattctaca gtaaatagct aggccatcct cgtctgcaaa gagagctaga tgaggctccg
    32281 aatgcaaaac attcaaacct acctctgact catcttcacg agagatatag ggtggattgg
    32341 atacaattat atcatatttt tcagaaattt ctgcaaaaca atcagatttt ttaaaaaata
    32401 tattaagatt ttgatcgcta gcattctctg atgcaagctc taaggcctct tgagaaacat
    32461 ctgctgctgt cactgaccaa tctggtctat tttttgctag agcgagagca atagctccac
    32521 tacctgttcc aatatccaga actgagagat tcgtctcaag attttcagtt agaataagct
    32581 ccactaactc ctctgtttct ggacgaggaa tcaaaacccg ttcatccacc tttaaatgca
    32641 ttccataaaa atctgcctgt ccaatgatgt actgagctgg cttgtgagct gctagttgct
    32701 ggtaaatatc ttctacaaat tgtttttctt cctctgttgt cacctcctgc tggagggcaa
    32761 aaataaagtc tgtaaaagat agatttttca gactacgata gacaaaagag aggctttccg
    32821 cttcctctcc ttgtcttatc aactcttctt caaaatttga aaataattga gctaatttca
    32881 ttatttgttt aattcttcta gtttttgtgt ttggtcataa agcaccaagg catccacaac
    32941 ttcgtccaat ttaccagaca aaatcgtatc tagtttttgg agggtcaagc cgatacggtg
    33001 gtctgtgaca cggttttgtg ggaagttata agttcggatc cgttctgaac ggtcaccagt
    33061 accgattgtc gacttacgct cagcgtcttg ttcatcctga gcaatctgag caaagtggtc
    33121 agcgacgcgt gcacggataa tcttcatagc cttctcgcgg ttcttctgct gggtacgttc
    33181 ttcctgcatc tcaaccttga tattggttgg caagtgaacg atacgaacgg cagtcgcaac
    33241 cttattgacg ttctgtccac cagcaccaga ggcgtgatag atgtcgacac gaaggtcttt
    33301 tggatcaatg tcgtattcaa cctcttcaac ttctggcata acaagaactg tcgctgtcga
    33361 agtatgaaca cggccttggc tttctgtcac aggaacacgt tgcacacggt gggcacctga
    33421 ttcatactta agcttagagt atacagactg acctgaaacc atagcaacca cttctttaaa
    33481 accaccgaca ccattcatag aggcttccat gacttcaaag cgccaacctt gggcttccgc
    33541 atacttttgg tacatagtta gcaaatctcc agcgaaaagt gccgcttcgt ctccaccagc
    33601 tgctccacgg atttcaagga tgatattctt gtcatcgttt ggatcctttg gaaggagcaa
    33661 aattttcagt ttttcttcat attcttcttt ttcagccttg gcatctttga gttcttgctt
    33721 ggccatttct tccaagtccg catctccgcc tgattcctta atcatctctt cggcatcgac
    33781 gatattttga aggacttgtt tatactcacg gtaggctatt acggtgtcac gattggaagc
    33841 ttcttctttt gaaagctcca taaaacgctt ggtgtctgaa acgacatcag ggtcactcag
    33901 caattctcct agttcttcat aacggtcttc tacagcttgt agttgatcat agatgttcat
    33961 ttttcttcat tctccttatt gatttcaggg gcaaaataat gtttacggca aaccgagata
    34021 taggtttcat taccaccaat ctggatctgt tctccatcgt aaacgggcag tccatcctgt
    34081 gttcgcaaca ccatggtcgc ctttttcttg caatactgac agatggtctt gatttcgtca
    34141 atcttgtctg ctaaaagcaa gagatatttg gaaccttcga acaattcatt gcgaaagtca
    34201 tttttcaagc caaaagccat gacgggtatg tctaactcgt ccacaacacg agctaggtca
    34261 taaacatggt ggcgtttgag aaactgggct tcatcgacca aaacacagta aggtttttct
    34321 ggtaggtctc ggatatagcc aaagatatcc gttgtttcct caatcgcaag ggcagggcgt
    34381 ttcatgccaa ttcgactcga cacatagcca acaccgtcac gcgtatccag agccgaggtc
    34441 ataatcacaa caccttttcc ttgctcctcg tagttatagg ccactttgag aatctcaatc
    34501 gttttaccag agttcatggt cccataacga tagtacaact gtgccatgtt tcttgcttca
    34561 cgtccatttc taaatttttg ctacattcta gtatatcata attttcttaa gctttaaacg
    34621 gcaaaatgtg gtaaaatgga agaaatcaaa actagtggag gaagctatta tgccatttgt
    34681 acgcatcgat ttatttgaag gacgcacgct cgagcaaaag aaagctcttg ctaaggaagt
    34741 aacggaagcg gttgttcgca acactggagc acctcaatct gctgttcatg tcatcatcaa
    34801 cgacatgcca gaaggaactt atttcccaca aggggaaatg cgcaccaaat aagctagctt
    34861 aagcagaatt gcttaggctt tttcaatctc caagtagcac ccattgaaga aatagtctaa
    34921 atttgttaca atttgaaaag agacttggaa aattccaaga aaagagcaat taattaaagg
    34981 aaacattatg attacacgtg aatttgatac catcgctgct atctctactc cactaggtga
    35041 aggggctatt ggtattgtcc gcctgagcgg aacagacagt tttgctattg tgcaaaagat
    35101 ttttaaagga aaagacttga acaaggttgc cagccacact ctcaactacg gtcacattat
    35161 tgatcctctg actggtaaag tcatggacga ggttatggtt ggggctatga agtctccaaa
    35221 gaccttcact cgtgaggata ttatcgagat taacacccac ggtgggattg cggtgaccaa
    35281 tgaaattctc cagctagcta ttcgtgaagg ggctcggttg gcagaacctg gtgaatttac
    35341 caaacgtgct tttttaaacg gtcgcgtaga cttgacacag gcagaggctg tgatggatat
    35401 catccgtgcc aagactgaca aggccatgaa cattgcggtc aaacaattag acggctccct
    35461 ttctgacctc attaacaata cccgtcaaga aatcctcaat acacttgccc aagttgaggt
    35521 caatatcgac tatcctgaat atgatgatgt tgaggaagct actactgccg ttgtccgtga
    35581 gaagactatg gagtttgagc aattgctaac caatcttctt agaacagccc gtcgcggcaa
    35641 aatccttcgt gaaggaattt caacagctat catcggacgt cccaacgttg ggaaatccag
    35701 ccttctcaac aacctcttgc gtgaggacaa ggctatcgta acagatatcg ctgggacaac
    35761 acgagatgtc atcgaagagt acgtcaacat caatggtgta cctctcaaat tgattgatac
    35821 agccggtatt cgtgaaacag atgacatagt ggaacaaatc ggtgttgagc gttcgaaaaa
    35881 agccctcaag gaagccgact tggttctact agtgctaaat gccagtgaac aactgactgc
    35941 gcaagacaga caacttcttg aaattagcca agataccaat cgcattattc tacttaataa
    36001 aaccgacctg ccagaaacga ttgaaacttc gaaactacct gaagacgtta tccgtatttc
    36061 agtccttaaa aaccaaaaca tcgacaagat tgaagagcga atcaacaacc tcttctttga
    36121 aaatgctggc ttggtcgagc aagatgctac ttacttgtca aacgcccgtc acatttccct
    36181 gattgaaaaa gcagttgaaa gcctacaagc cgttaatcaa ggtcttgagc tggggatgcc
    36241 agttgatttg cttcaagttg acttgactcg tacttgggaa atcctcggag aaatcactgg
    36301 ggatgctgct ccagatgaac tcatcaccca actctttagc caattctgtt taggaaaata
    36361 agaaaaatcc atgatccttc attcggtcat ggattttatt gtctttatta gtaatctggt
    36421 cttaagaccc ctgttacagt tgccttagtt gcttcgtagt cgccatctac gacaaccttg
    36481 ataatgcgtt tggcatcttc ttctggtgct ggaacaagag gtagacgagt gggtccagct
    36541 tcaaatccca tatagttaag aattgcctta actggagcag gacttggata agagaagaga
    36601 gcattaacct taggaatgaa tttacgctga attgctgcgg ctttcttcat atcgctttct
    36661 gcaatggcag taaacatctc gtgcatttca tccccatttg tatgagaggc aacagaaata
    36721 accccatccg ccccaaggtt catggcatgg aaagcatctc catcctcacc tgtataaatc
    36781 aagaactctt caggcttgtg ctcaatcaag taagccatat tagccaagct agtacattct
    36841 ttgacaccga taatatttgg atggtcagcc aagcgaagca tggtttctgg agtcaattcg
    36901 acaactacac gccctggaat gttatagata ataattggta ggtcagaagc atctgcaata
    36961 gccttaaagt gctgatacat cccttcttga gaaggtttgt tgtagtaagg aacaatagca
    37021 agcccagctg cgaaaccacc aaattccgct acttctttga caaactcaat agagtcacgc
    37081 gtatcattag tacctacacc cgcaatcaaa ggaacgcgtc cattgacaac cttttgtaca
    37141 gccgcaaaca actccaactc ctcatcgtgg gtcaaagttg gactctcagc agtcgttcct
    37201 gcgagaagaa ttccatccgt atgatgggcc aataaatgct caatcaaggc tggaatagca
    37261 tcaaagttaa tggaaccatc ctcatggaag ggggtaataa aggctgtaat gattttacat
    37321 tcttttaaat cttgataaga cacgaaaaca cctctctatt tcaaagaagg agttcatctg
    37381 aactcctaaa cgatatgact attttaattc aaatttcaat tcagctgttg gacgaaccaa
    37441 tccacgttca tgaagagttt cagcaatctg aactgagttc caagcagcac ctttgagaag
    37501 gttatctgaa acaacccaca tgtgaattcc tttttctgca tccaagtctt tacggatacg
    37561 accaacaaag gtatcacgtg aaccaactgc attgatggct tgaggataga tttgatgaac
    37621 tacatcatct tcaagaacag cacctgggaa ggatgcgata gctgctttga cttcttcaat
    37681 tggagccact tcctttgttt caatataaac tgactcagag tgagctgaca agactggaat
    37741 acgcacacat gtcgcagata ctgcaatgct atcatcttcc ataattttct tagtttcctt
    37801 ggtcatcttc atctcttcgt acgtgtaatc attatcagtg aagacatcaa tttgtggaag
    37861 agcgttaaag gcgataggat aatgtttctt gtcaccacct gaaggcaaga tttccgcatg
    37921 caaatcacgt ggtttcactc catcattcaa gacctcacga agttcacgtt gtgtctcaag
    37981 aattgctccc ataccagcac ctgaaacggc ttgataagtt gaaacaatga tacggtccaa
    38041 gccccatttt tggcgaaccg gctcaagagc caccatcatt tggattgttg aacagttagg
    38101 gcaggcaatg attccgttgt gggcatcaag tgcatgagca ttgacctctg gaacaaccaa
    38161 aggaacatct ggattttgac ggaaataaga tgtattatcc actactacca cgccagcttt
    38221 tactgcgtat ggtgcatact tagctgatgt cgaaccacct gctgaaaaga gagcaatatc
    38281 aactccttca aaagctgttt cagtcgtttc ttcaatcgta atatcttgat ctttaaattt
    38341 caatgactta cctgctgaac gtgcagaagc aaggtaacgg attttatcga ttggaagtgt
    38401 tgattcttcc aacattttta tcatctgagc accgacagca cctgtcgcgc cgactacagc
    38461 aactgtatat cccataaata acctctttcg gaattttcta aaaatttcta taatagaagt
    38521 attatactac tttttccatg aattgcaaag ctttttcatc attttttgga aaataaaaat
    38581 ccccaatcgc tagactaggg attaagaggc atcttcatgt gatgagcagg ttcacacaac
    38641 tcatcaaggt ccgctcctgc gttatgacct ccttatgctc aatagtagcc cgaagactac
    38701 ctatcgactc atcgcatcta ctattctact agatagagtc atttttgtca acagccaaaa
    38761 ctctttatac tcttcgaaaa tctcttcaaa cttcgtcagc gtcgccttgc cgtatgtatg
    38821 gttactgact tcgtcagttt catctacaac ctcaaaaaca tgttttgagc tgacttcgtc
    38881 agtttcatct acaacctcaa aaacatgttt taagctgact tcgtcagttc tatctacaac
    38941 ctcaaagcag tgctttgagc aacctgcggc tagtttccta gtttactctt tgattttcat
    39001 tgagtattag ttttatttac acaggtgcat aagaaatata ttggaaaaat tgggggcaga
    39061 cataacaaaa acccttgtaa atcaagggct tttctgtcta tttaatgcta attgccggga
    39121 ttgaaccggc gacctcatcc ttaccatgga tgcgctctgc caactgagct aaatcagctt
    39181 acttaagaag tatactatat tcagaaaaac ttgtcaagtt tccttagaaa ttttccatag
    39241 gaaaaagcca ggtaagaact acctagctta tcttcttcta tttacttaaa tcaattcgac
    39301 gaccaatttt accaataata atcgctccaa taatcaatga agcaaattca ccgattcctg
    39361 ttgtaaacca agtaaggaag aatggtaatt ctgctacaat atttaactct gcagcgatag
    39421 taaacatgga aattgaaaag aggattgaaa agaagaaatg atcttttcga atcaatccgt
    39481 taaagaggta atccttgcta tattttgcaa aaagccaaac acctaaactg aggaatacta
    39541 aggttgaacc accaccaaca aagacatcta gcagtccgaa gctaaagaaa ttagcaatca
    39601 tacaaccgat ggtaactccg atgatgtact taggattgta aaaagccata aagttcatca
    39661 tttctgaaat acgaaactga taagcaccat agctgatggc atttaggggc ggtgtgacag
    39721 tcaaaacgac gtagatagca gcgacgattg caatatctgc aacatcacga atagttaatt
    39781 ttttcatctt ttctcctttg gcggttatcc gcgtgtaata tgcttggtga aagaagctaa
    39841 gcaccaaggg ttgattcatt caaccttact agtatagcac aatagcctgc tttatgctat
    39901 acttaaagca tgaaaaaacc tattcaaaaa ttttttaaca atgaaatctt agcctatctc
    39961 ttttttggcc ttgcgacaac tctagtttcg attctctcac gactagtcat ttatcaacta
    40021 agtcatcagg aactccttgc tacggccttg gcaaatatta tcggtatctt atttgccttt
    40081 atcacaaatg atacgattgt atttaagcaa aaaaagaaaa atagacttat ccgtttagtg
    40141 aaattttctc ttgctcgcct ttctactttt ctgttagact tgtttttaac ttttctcttt
    40201 gtaacccaat ttcctcacat catagggcaa ttcgtaactg aaaacattga taagataaat
    40261 gctattgaaa caataattgc acaaatattc attattatca tcaattatat cttgagcaag
    40321 gtctatattt ttagaaaata atgctccatt tatgagcctt ttaattgcct tctataatga
    40381 tttaggatac aatgaaacac gaaacattga aaggaaaaca gtatgttaaa aaatctaaaa
    40441 tcgttcttgc ttcgaggaaa tgttattgac cttgctgtcg gtgttgtcat tgcctctgct
    40501 tttggtgcta tcgttacttc acttgtaaac gacattatca ctcctcttat tttaaatccc
    40561 gctttgaaag ctgctaaagt cgaacgtatc gctcaacttt cttggcatgg agtcggctat
    40621 ggtaactttt taagtgctat tatcaatttt atctttgtgg gtaccgccct cttctttatt
    40681 ataaagggca ttgaaaaagc acagaaactg actggcataa agaaagaaaa aactgccgaa
    40741 aaaaaaccaa ccgaattgga agtccttcaa gaaataaaag ctctccttga gaaaaaataa
    40801 tcgattaaaa ggatctagcg caaagctaaa tccttttttc tttactcttt gggtaacttg
    40861 cctgcttttt caagcatttt tttgataaga taaggcatct taacattctc ccgccctttt
    40921 ttctcaatca gttttttcac aaattctggc atttgtaaat cttcagattc agctaaaatg
    40981 tttttcgtct cgtctgaagg aactaactca tcaaaggtct cttcttctgg gagaaattcc
    41041 ttaaactctt gattttcatt gactctaact gtattgacca aagcatcaat caaacgagaa
    41101 tctgtatttg gcattggtgg acgatggtag tttaccccaa attcctggca taagtcataa
    41161 cattctacat cattgtcaaa caagacctca atatgctcac tgatgaaact aataggtaca
    41221 aaaatataat ggtctggatg ttctgtctgt tctctgagat actccaaaac atctggttta
    41281 atccatggaa taccaatatc actctcactc tgccaagtgt tggtatattg ttctgaactc
    41341 aagcccagtt tttcagcgac tagcttgcta ttttcgaaaa tttggtctat ataaggatca
    41401 ccaaagtcca aggcaaaaat gggcacacta tgggctgaaa agatgacttt aaagctgtct
    41461 tgctttactt cttcttttaa aatcttagca atttcatctg cccaatagtt taagagcgct
    41521 tcttcctgat accagtcctt aatgaccaaa aattgaattt gtttgctttc taaaaatttt
    41581 tcatagccca tgacagagta gaaagaataa tggggctcca aaatcaagca aatacactgt
    41641 tcaattccgt ctgcttccat ttgaccgatc acatctggaa taaagggact ggaaaactta
    41701 ttagcaaagt agacactgta ttcattcccc agcctagctt ctaccaaggc aacctcttga
    41761 cgggtaattt tttgcagagg cgtgcctcca attcgtacat aattatcata gagagtctga
    41821 atctcgtggt cttggggtct caccccacga cgaatatttg tgaaaaaatc agctacacct
    41881 tcaaaggtaa tctcttctgg tgaaccgaag gtcatcatta aaattgcttt tttcatgact
    41941 gcttccttgt gatgttttta tcaagtttat tttatcatta attaatcgat aagtaaacgc
    42001 taatataggg acattctcaa tcttctgtct tttgtttttc tcttctttct atgatacaat
    42061 ggaaaaaata aattcaaaag gagttttttt atgacttatc caaatctctt ggaccgcttc
    42121 ttaacctacg ttaaggtcaa cacgcgctct gatgaacact ctactactac tccaagtaca
    42181 cagagtcagg ttgacttcgc aacaaatgtc ctaattcctg aaatgaaacg tgttggactg
    42241 caaaatgttt actatctacc gaatggtttt gctattggaa ccttgccagc caacgatccg
    42301 tctttaacac gtaagattgg ttttatatcg cacatggata ctgctgattt taatgctgaa
    42361 ggagtcaatc cacaggtaat tgaaaactac gatggtggtg tgattgaact agggaattct
    42421 ggtttcaaac tcgatccagc tgacttcaag agtcttgaaa aatatccagg acaaacgctc
    42481 atcacaacag atggaacaag cttgctaggt gctgatgaca agtcaggaat tgctgaaatt
    42541 atgacagcca ttgaatatct aactgctcat cctgaaatta agcactgtga gattcgtgtt
    42601 ggttttggtc cagatgaaga aatcggtgtt ggtgccaata aatttgatgc agaagatttt
    42661 gatgtggatt ttgcctacac tgttgatggt ggtccactag gtgaacttca gtacgagact
    42721 ttctcagccg ctggtgctga attgcatttc caaggccgta atgtccaccc tggtactgcc
    42781 aaagagcaga tggtcaatgc ccttcagcta gcaattgatt ttcataatca acttccagaa
    42841 aatgaccgac ctgagttaac tgaaggttac caaggttttt accatctaat ggatgtgaca
    42901 ggtagtgttg aggaggtgcg tgcaagctac atcattcgtg attttgaaaa agatgccttt
    42961 gaagcgcgta aagcatccat gcaatctatc gctgataaga tgaatgcaga acttgggagc
    43021 taccgtgtca ctctcaactt gacagaccag tactacaata tgaaagaagt cattgaaaaa
    43081 gatatgactc caattaccat tgctaaagcc gttatggaag atctaggtat cacgcctatt
    43141 atcgaaccaa tccggggtgg aacagacggc tctaagattt cctttatggg aatcccaact
    43201 ccgaatatct ttgcaggtgg cgaaaatatg cacggacgtt ttgaatacgt tagccttcag
    43261 actatggaac gtgcagttga taccatcatt ggcatcgtag cttataaagg ctaaaaagac
    43321 gaggtagctc agctacttcg cctttctttt tattctactg gtttttcttg atttccagta
    43381 gttgtagaag attctgttgt ttcattttct gaagttgatt cagcaggttt agaatctctt
    43441 gtattgcttg gtttgttttc gtcgctagca atttcaatgt tagattctgc agttgcgttt
    43501 ggttggttct cagcactggt gttatcacta tttgcttcag catttcttgc tggacttgtt
    43561 tcttcacttg cgctagcttt tgactggatt tgatgattca aaactagaat agcttttgtc
    43621 gattcaagta aagctgtttt gtctttactc ttagcagaaa gttgatctaa taatgcatcc
    43681 accttatcaa agtccgcatc agatccatta ttactttcta aataagagtg aagcgacatg
    43741 agaatatcgt agagtttttg atagagtaca agtgtctgag aatcttgctc agcattttcc
    43801 ttttcttgtt gaagggcgct agcgatacga gtcaagacat cttttacctg actgtttact
    43861 tcatccaagt ctgcatcagc cttgtttgtg gcagctttta gattttctac ttcttctgcc
    43921 aaggattgtc tgattccttc ttcatggatt tgttccaaga gttgatttac cttgctcaaa
    43981 agactttcta cttcttcctt gctatctgtc gcagattatt ggttgccatc taccatgtac
    44041 tcctaaaaca ggagagttat aatccaagat tacaaggcct tacagaaata agaaatccag
    44101 ataagacaat gttcgtccaa gacgctattc gcttcgcaca gcagcacgga ttcaatatgc
    44161 tttaatttta aagtttaggt gtcaagacct ctttttagtg tgcccaaaat ttagagaagt
    44221 aatcaatcaa ctaactttta tttttttcaa actttcagta aactgaccta aagctaactc
    44281 aatctgtctt tgtagatgct tctgctatca gctagaagtt gatctacttt tgccaagact
    44341 gccttctcat caaaagttcc aggttgatag ttggattgca gggatggaat cttgtttttc
    44401 aaagccgctt catatccctt agtttgaacc ttgatgtagt gattgtggtc gccatgagga
    44461 atcacaaaac cttctgaatc ttcacttata attcgattgg catcaaaacc atgaccatct
    44521 tcttcctcat gatggacatg tagtgacgga ttacttaata cagaactaga agaacttcct
    44581 acctcttccg tgttagagtg tgatggggga ttgttaagag atgacttagg aatatagtga
    44641 tagtgatccc catgtcttac tatataagca tcacctgtat ctctgacaat atcattaggg
    44701 ttaaagacat atgtggctgc taattcacct gccgacaagt cactctcagg aatgaaatga
    44761 tagtgaccac catgtggtac tatagtagat tgaaatagaa tatgagcaaa ttgataaggg
    44821 gattttaaag taatttctaa caatgattta gaaactatga tgtgctattc taaattcaac
    44881 tcactatata taaccatcat cggtagtata acgtccctgt aattttgcta cagatacttc
    44941 tgcactagct cctttatcgt ctttaccatg ttcttgtttt tggcgattga tttcatcttt
    45001 tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa acataatact ttccatctac
    45061 cttaataata tatcctccct taacctaact gacgatatct tgatctttcg gctgatagtt
    45121 gggggctttc attaatagct cttcactaaa gagcgcatca aaaggaactt taccattata
    45181 gtagtgataa tgatcgccat gagaagttac ataaccttga tctgtaatct taataacaat
    45241 ttgttttgct tgaattcctt ctttttgact aacctagtct ggagtcaaat tttcagtctt
    45301 cttagtgtct ttattactgt ttacatatga aacacgattt ttatctgtat tggcctgtta
    45361 gctatgttgg ttcagagcat aaacacacag acttaaggaa aggataacaa cagatccagc
    45421 tgctatatat ttctttttaa atttcataat tacctcattt ctataattat ttatatgatg
    45481 tcttcattat taaatgatta aataaattaa ttaaccaatt aattaactag taaatattcc
    45541 acctcttttt aagttgtatg tcaagaaatt ttatatatta ataataaaat gaaattctcc
    45601 caaagtcaga gttttatttc taacttttga gagaacttca tttttgattc agactttttc
    45661 tactgctatt ccttacgcta tgagatcaga taaattcttt tttatcactt ctccacttgg
    45721 caatcttaat tcaatcgttc catccatatt gaatataaca ctatctaagc ctaatccgta
    45781 actagctgta aatttttcta atttttcttg tacaggatct actgctggag cttcctctaa
    45841 tgctggatct aacatagggt cactccccac attcccttct ggattcaaca ttccattatc
    45901 cgttgagttt tctggtttta caggtttttc gtttggtgcc tctggtaaag aatctgctgg
    45961 tttattttct gttggttggt tctcaactgt tccagtagat acttttccat tttcagatgg
    46021 tttattttca ccatttcctt gaggtgcttc tcctgtaaaa tctgccatat tctttttaat
    46081 gacttctccc gatggtaaat ataattcaat tgttccgtcc atattaaaca agacattttc
    46141 tagcttcatc ccataacttt cagcaaattt tgctactttt tcttgtacag gatccactgt
    46201 aggaacttct tctaacgttg aattactagt actattccca gtttcagaaa gtttttcttt
    46261 ttctaccttc tcactagtct ttggttcttc taccttttca tcaagttttg agttttcttg
    46321 tgctttattc cttttaaatt gtggtagaat acttggttta tcagtttgat tttctttttc
    46381 caagataggt acttccacaa tataagtcga ttgattgtcc aaataagcat ttgccatgaa
    46441 ggttacagga attttatttc cggccgttct ggttgttcct tggtttaatt tcggaatcgg
    46501 taatttgatt tcaccaactt tatagttatt ttctaaataa gcatttccat gaaattcatc
    46561 aaacactctg actaaagcat cagttccttt aggcactgca aattgagggt tcactcttaa
    46621 ataagtatcc cctgcatgga aaggatagaa aatcgtttga ctggccattt tgtaagctaa
    46681 agaggttgga actgtaaatg taccatcata acttacttct ggataatctt ttgaagcgat
    46741 agtatactta aatgtttgtc ctggtaaata aggttgatct aattcaaagt ttgcaatatt
    46801 ccctactcct tctccaaata ctttaccaga tactttctcc aatacttttc catctggtgt
    46861 tattaatttt actagcatat tgatacctaa ttttttctcc aattcaggcg gaaaactaaa
    46921 agaaacgcgt ttttgaccat tggctagagt aaagttttga ttattaaacg tactattttt
    46981 taacaaatta acaacattcg ttaattcttc tccagtataa actttattcc cttctttttt
    47041 agcaactcct tcttcgggtt taaacagttc atagttactg tgagaatgac caattccaac
    47101 cggtttatgt tcatcaatcg gatctgcatg atggtgatct ccatgcggat aaataatcgc
    47161 atttttttct ttattcacga caatactttc acgtttgaca ccatattgtt tcataatgcc
    47221 agcaattttt tcttcgattt ttttatctaa atctttcatt tctttggcat tacttggata
    47281 atcctgttca tgagatgaca aagaatctaa tccattatga ctagttttaa cttcctctaa
    47341 atgtttttgc gcagccttaa tttgctcttc tgtcaagtcc ttcttgaaga aataatgatt
    47401 gtggtctccg tgactcatga caaaacctga ttcatcttca gcgataatac gattagcatc
    47461 aaatccgtat ccatcttctt catgtttctc atgtgaagtt cctggattga ttggaagaga
    47521 tggagaaggt gttgctagac tattgtttgg aagagtcggt tgcccaattt gatttgattt
    47581 tggaatgtaa tggaaatgat caccatgtct tacaatataa gctgtagccg tttcttcaac
    47641 gatatctttt ggattaaaaa tataaccatc agatgctgaa gagagctcct tacttgtcgt
    47701 taaagaagaa ggattgcttg aaagactgcc tagactagac actacttcat taggttttgc
    47761 atttgtagaa actgtagaac cagttccact gataggcacc attctggcaa tcttttcttc
    47821 taaggcagaa agcttgctgt aaggaataaa gtggtaatgg tcgccatgcg gaatcgcaac
    47881 tccatttggt gtacgactga taatcttagc agggtcaaag accaggccat ctgattcact
    47941 gtaacgttgg gcgctaggtg aatcatagag ttccttcaaa agactctgga gattttcaga
    48001 tttatttgct ggcttgctag ttgatccttt tgctacagat tgcgtgttat tgtcactagc
    48061 tgttgaagaa tagcttaact gactcggttg catatttttt ccagccagat gtgctttagc
    48121 tgctgctaat tcactagcag ataaatcgct tttgggaatg tagtgatagt gacctccatg
    48181 aggaacgata taagcattac ccgtatcttc gataatatca gctggattaa agacataacc
    48241 atcatttgtc gtatatcgtc cctgagacct tgctacagca acattagagt taaccttctc
    48301 attatctttg acatgttctt gtttttgacg attgatttca tctttagttc gaacattatc
    48361 agcatgagct gcatctttca ggtagacata atattttcca tcgaccttga tgatataacc
    48421 acccttgact tcattgacaa tatcagcgtc tttaagttga tagtttggat ccttcatcaa
    48481 gagttcttca ctaaagaggg catcataagg aactttccca ttatagtaat gatagtggtc
    48541 accgtgtgac gttacatagc cctgatctgt aattttgatt acaatttgct cagcctgaat
    48601 tccttctttc tggctaacct ggtctggtgt caagttttca cttttctgac ttgactggct
    48661 gccatccaca taagagacac gattattgtc cttattttcc tgcgaacgat gctggtttag
    48721 tgcataggca catagactca aggatacgat aacagctgat ccagctgcta tatatttttt
    48781 actaaatttc ataaatccct catttcaata aatgatgaag ttttttctca acttctttta
    48841 ctttattaaa tagttttcta aaccggttat ttaaccattt aattaaccag taaatagttt
    48901 actatttaaa attacttttg tcaagtttct gttacgtttt tctaaaaaag agaagtaaca
    48961 cgaaaggtta cttctccata aaatatgctc cattcatttt actactgtat aggagccggt
    49021 tgactttttt ttaacaaagc caagagacta tctacttctg ctgaaatagt gttattatct
    49081 ttcgttccga gaagaagact actttttaga ccagtcaatg tctccatagc attttgtcta
    49141 atactaggat ctgttacttt ttctagcaag gcctccgcat ctgctatctt agcgttaata
    49201 acagaatgct ctacttgagg aatttcagcc tcatctgtag tatcttcagc ttcttcctct
    49261 gtctcttccg tatcagtgct tggtttataa agattatctg ctgaaggatt taaaccagcg
    49321 tgattctctt tttcatcaga ttcagggtga gttggctcac ttacttcatc atgtccctta
    49381 tcttcatcag gtttactgtc ttggtctgc
//
LOCUS       DBEJAQ010000007        48743 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.007, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000007 DBEJAQ010000000
VERSION     DBEJAQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 48743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.007"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(46..1935)
                     /locus_tag="ACL297_000475"
     CDS             complement(46..1935)
                     /locus_tag="ACL297_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGR0070788.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNFFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2073..3638
                     /locus_tag="ACL297_000476"
     CDS             2073..3638
                     /locus_tag="ACL297_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGR0070789.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3638..4378
                     /locus_tag="ACL297_000477"
     CDS             3638..4378
                     /locus_tag="ACL297_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGR0070790.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4487..4702)
                     /locus_tag="ACL297_000478"
     CDS             complement(4487..4702)
                     /locus_tag="ACL297_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070791.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5573..6823)
                     /gene="ilvA"
                     /locus_tag="ACL297_000479"
     CDS             complement(5573..6823)
                     /gene="ilvA"
                     /locus_tag="ACL297_000479"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HGR0070792.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6873..7229)
                     /locus_tag="ACL297_000480"
     CDS             complement(6873..7229)
                     /locus_tag="ACL297_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGR0070793.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(7467..7703)
                     /locus_tag="ACL297_000481"
     CDS             complement(7467..7703)
                     /locus_tag="ACL297_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070794.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7810..8832)
                     /gene="ilvC"
                     /locus_tag="ACL297_000482"
     CDS             complement(7810..8832)
                     /gene="ilvC"
                     /locus_tag="ACL297_000482"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGR0070795.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAETTKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8898..9374)
                     /gene="ilvN"
                     /locus_tag="ACL297_000483"
     CDS             complement(8898..9374)
                     /gene="ilvN"
                     /locus_tag="ACL297_000483"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGR0070796.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9367..11067)
                     /locus_tag="ACL297_000484"
     CDS             complement(9367..11067)
                     /locus_tag="ACL297_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGR0070797.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11267..12934)
                     /locus_tag="ACL297_000485"
     CDS             complement(11267..12934)
                     /locus_tag="ACL297_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGR0070798.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12937..13302)
                     /locus_tag="ACL297_000486"
     CDS             complement(12937..13302)
                     /locus_tag="ACL297_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGR0070799.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13459..13647)
                     /gene="rpmB"
                     /locus_tag="ACL297_000487"
     CDS             complement(13459..13647)
                     /gene="rpmB"
                     /locus_tag="ACL297_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGR0070800.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13766..14248)
                     /locus_tag="ACL297_000488"
                     /pseudo
     CDS             complement(13766..14248)
                     /locus_tag="ACL297_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
     gene            complement(14517..16061)
                     /locus_tag="ACL297_000489"
     CDS             complement(14517..16061)
                     /locus_tag="ACL297_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGR0070801.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16274..16576
                     /gene="gatC"
                     /locus_tag="ACL297_000490"
     CDS             16274..16576
                     /gene="gatC"
                     /locus_tag="ACL297_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HGR0070802.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16576..18042
                     /gene="gatA"
                     /locus_tag="ACL297_000491"
     CDS             16576..18042
                     /gene="gatA"
                     /locus_tag="ACL297_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HGR0070803.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18042..19484
                     /gene="gatB"
                     /locus_tag="ACL297_000492"
     CDS             18042..19484
                     /gene="gatB"
                     /locus_tag="ACL297_000492"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HGR0070804.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19613..20173
                     /gene="efp"
                     /locus_tag="ACL297_000493"
     CDS             19613..20173
                     /gene="efp"
                     /locus_tag="ACL297_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGR0070805.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20195..20584
                     /locus_tag="ACL297_000494"
     CDS             20195..20584
                     /locus_tag="ACL297_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGR0070806.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20577..20999
                     /gene="nusB"
                     /locus_tag="ACL297_000495"
     CDS             20577..20999
                     /gene="nusB"
                     /locus_tag="ACL297_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGR0070807.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     AVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21048..21242)
                     /locus_tag="ACL297_000496"
     CDS             complement(21048..21242)
                     /locus_tag="ACL297_000496"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGR0070808.1"
                     /translation="MILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFFNFL
                     NHAKPIWIFVYNYIYYHFFR"
     gene            complement(21297..21748)
                     /locus_tag="ACL297_000497"
                     /pseudo
     CDS             complement(21297..21748)
                     /locus_tag="ACL297_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21766..21951)
                     /gene="briC"
                     /locus_tag="ACL297_000498"
     CDS             complement(21766..21951)
                     /gene="briC"
                     /locus_tag="ACL297_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HGR0070809.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22110..22877)
                     /locus_tag="ACL297_000499"
     CDS             complement(22110..22877)
                     /locus_tag="ACL297_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGR0070810.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(22874..23740)
                     /gene="accD"
                     /locus_tag="ACL297_000500"
     CDS             complement(22874..23740)
                     /gene="accD"
                     /locus_tag="ACL297_000500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGR0070811.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23777..25144)
                     /gene="accC"
                     /locus_tag="ACL297_000501"
     CDS             complement(23777..25144)
                     /gene="accC"
                     /locus_tag="ACL297_000501"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGR0070812.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASRNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25156..25578)
                     /gene="fabZ"
                     /locus_tag="ACL297_000502"
     CDS             complement(25156..25578)
                     /gene="fabZ"
                     /locus_tag="ACL297_000502"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGR0070813.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(25575..26060)
                     /gene="accB"
                     /locus_tag="ACL297_000503"
     CDS             complement(25575..26060)
                     /gene="accB"
                     /locus_tag="ACL297_000503"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGR0070814.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26063..27298)
                     /gene="fabF"
                     /locus_tag="ACL297_000504"
     CDS             complement(26063..27298)
                     /gene="fabF"
                     /locus_tag="ACL297_000504"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGR0070815.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27320..28051)
                     /gene="fabG"
                     /locus_tag="ACL297_000505"
     CDS             complement(27320..28051)
                     /gene="fabG"
                     /locus_tag="ACL297_000505"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGR0070816.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28085..29005)
                     /gene="fabD"
                     /locus_tag="ACL297_000506"
     CDS             complement(28085..29005)
                     /gene="fabD"
                     /locus_tag="ACL297_000506"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGR0070817.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTSLLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTTHLAHVEDQASLVALLEK"
     gene            complement(28998..29972)
                     /gene="fabK"
                     /locus_tag="ACL297_000507"
     CDS             complement(28998..29972)
                     /gene="fabK"
                     /locus_tag="ACL297_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGR0070818.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(30091..30315)
                     /locus_tag="ACL297_000508"
     CDS             complement(30091..30315)
                     /locus_tag="ACL297_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGR0070819.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30375..31349)
                     /locus_tag="ACL297_000509"
     CDS             complement(30375..31349)
                     /locus_tag="ACL297_000509"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGR0070820.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31349..31783)
                     /locus_tag="ACL297_000510"
     CDS             complement(31349..31783)
                     /locus_tag="ACL297_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGR0070821.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31877..32662)
                     /locus_tag="ACL297_000511"
     CDS             complement(31877..32662)
                     /locus_tag="ACL297_000511"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HGR0070822.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33031..34395
                     /locus_tag="ACL297_000512"
     CDS             33031..34395
                     /locus_tag="ACL297_000512"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGR0070823.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34398..34769
                     /locus_tag="ACL297_000513"
     CDS             34398..34769
                     /locus_tag="ACL297_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HGR0070824.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            34983..36257
                     /gene="serS"
                     /locus_tag="ACL297_000514"
     CDS             34983..36257
                     /gene="serS"
                     /locus_tag="ACL297_000514"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGR0070825.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVEAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36282..37181)
                     /locus_tag="ACL297_000515"
     CDS             complement(36282..37181)
                     /locus_tag="ACL297_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGR0070826.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLTE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37291..37839)
                     /locus_tag="ACL297_000516"
     CDS             complement(37291..37839)
                     /locus_tag="ACL297_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGR0070827.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38057..39379)
                     /locus_tag="ACL297_000517"
     CDS             complement(38057..39379)
                     /locus_tag="ACL297_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGR0070828.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFTFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39470..39558)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39722..42058)
                     /locus_tag="ACL297_000518"
     CDS             complement(39722..42058)
                     /locus_tag="ACL297_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGR0070829.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKGQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQIGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42162..42710)
                     /locus_tag="ACL297_000519"
     CDS             complement(42162..42710)
                     /locus_tag="ACL297_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HGR0070830.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(42707..43009)
                     /gene="zapA"
                     /locus_tag="ACL297_000520"
     CDS             complement(42707..43009)
                     /gene="zapA"
                     /locus_tag="ACL297_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGR0070831.1"
                     /translation="MANLNRFKFTFGKKTLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43096..43977
                     /gene="rnhC"
                     /locus_tag="ACL297_000521"
     CDS             43096..43977
                     /gene="rnhC"
                     /locus_tag="ACL297_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HGR0070832.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            43981..44595
                     /gene="lepB"
                     /locus_tag="ACL297_000522"
     CDS             43981..44595
                     /gene="lepB"
                     /locus_tag="ACL297_000522"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGR0070833.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            44829..47195
                     /locus_tag="ACL297_000523"
     CDS             44829..47195
                     /locus_tag="ACL297_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGR0070834.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKNNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNSIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47243..48526)
                     /gene="tig"
                     /locus_tag="ACL297_000524"
     CDS             complement(47243..48526)
                     /gene="tig"
                     /locus_tag="ACL297_000524"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGR0070835.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        14275 a        11188 c         9197 g        14083 t
ORIGIN      
        1 ctatcttcca ttatatcata ctttttcagt ctatggtagc tctctttaaa aggctccgat
       61 actgccgcca cctccgcctc cagagaagcc accgccagaa cttccacttc cagaagatac
      121 ggagtaggta cttgctgtat ttgcgacact agcataatgg ctcatttgcg cgcttgaatg
      181 ataaaacata ctgtgccagc cataagctac atagagattg atatctggat tttcaacttg
      241 aatctgatga accttcatca aatgactaac cttgtccgca tagccaaata aggttgcata
      301 gaccaagaga cgattccaaa caacaatact ttccaattca gccttatcca gtcgtgcaat
      361 ctcacgtaac atattttcaa aactggtcca gagatagtag acctcagctc ccgcttcatt
      421 tagaacacca tcacgattat ctagtcgaag cttccaataa tagaaaacag acaaaactaa
      481 ccctagaaaa ccaagtattg gcaaagggag gtaaagatag ccatgaacgt ctaaactgta
      541 caagaacaaa ccaaatccga taaatagggg caagatagtc aagacaccca tacccacttg
      601 caaagtcttt tccaaaccag ttaaaggacg atagtaatct gggagccccc agaaggaaac
      661 tcgttttctc actccttctt gcatctggtt caatacctct tcaaaagaag atttgagttg
      721 aagccctctt gcttgaatcc gtttttcatc agaaactttg gctctacgat aaagactatc
      781 agataccttg taatccgcaa aaaaattgga aagagtggct tcttttttgc ctgaaaaggc
      841 tagatttaga cagtctctct caaatcttga caaaccatct tctttcacca atctcaagcc
      901 aactgcatct ccttctgaaa taatagagac attcccacgg tctatcacat ctagcaaggt
      961 agcttgaata agttggtcaa aagtgaattt gccagccccc ttgaccaagg gactcacttc
     1021 ctccaaggag gtcgagtaga ctgcttctga taaaaccata ggctctaatt ccattggtgg
     1081 ttcatagaga cgatgatttt tggcatattt gactgaagga gtggtctttc ttctataaat
     1141 aaaatagaag cagacactca ataacaagga gatagaaagt atcgaaggga ggacccaagt
     1201 aacgagttgt ttactctgat ctttttctct aacaatcgag tcttctatct tattaaactc
     1261 ttctaaacga ttccctttca agccctgatc cctagcgcta gcaaaatcgg tccgagacca
     1321 ataggcatgc aactcaactc cacgcttagc cggaagattg tctaaacgga tagtataatc
     1381 aaggttactc ttttcaatcg ttccctctct aaaaagtttc cctgtatgga aaaagagttt
     1441 ctcagccccc ttgtctcccc ttacatgaaa ttcaaacttt tcaatagact ctgaactatc
     1501 tgtcagaggt tgccaattta attcagcgat atcatcataa aggaaaagta aattttttaa
     1561 gttccagacg aggtcaactt caactatgtc gccctcctga cctggattat agactctcac
     1621 agtataacca tccgcttctt ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt
     1681 tttcgcagcc tgaacctttg gatgagggtc aatgtcaaac ccgctaggca tcttaccagc
     1741 acgtccaagt cccacgattt ggcccttaaa gtcctcctca aactggtaaa ctatcttctg
     1801 tctaaactct gccgtattat ctgcatgaat atacaaatcc ccttgataag agtttatctt
     1861 gaaatcaatg gcaaaaacag agagtggaag aaggcaaaac aagcctaaca ccagtaagaa
     1921 aaaagttttt ttcatcaact aagccccctt tttatctttc ttatcattat agcatctttt
     1981 cgtaagtaaa ggcttactta tgttgaaaaa ttacgcattt ttcgataaaa taggagacag
     2041 ttatttttta atagatcaga ataggagaaa tcatgagaaa aatataccta tctattttca
     2101 caagtctctt gctgatgcta ggacttgtca atgttgctca agccgatgaa tatttacgca
     2161 tcgggatgga agcagcatat gctcccttta actggaccca ggatgatgat agcaacggag
     2221 ctgtcaaaat cgatgggacc aatcagtatg ccaacggata cgatgttcaa atcgccaaga
     2281 aaatcgctaa ggacttaggt aaagaacctt tggttgttaa aaccaagtgg gaaggtctag
     2341 tccctgccct tacttctggt aagattgaca tgattatcgc aggtatgagt ccaactgcag
     2401 aacgcaaaca agaaattgcc ttttcgagca gttactacac tagcgaacca gttttgcttg
     2461 tcaaaaaaga ttctgcctac gcaagtgcta aatctttgga tgactttaat ggtgcgaaaa
     2521 tcacttctca acaaggtgtc tacctctacg acttgattgc acaaatccca ggtgctaaaa
     2581 aagaaacagc catgggagac ttcgctcaaa tgcgccaagc tcttgaggct ggtgtcattg
     2641 atgcttatgt ttctgaacgt ccagaagcac tgactgctga agctgcgaac tctaagttca
     2701 agatgattca agtagaacct ggtttcaaaa ctggggaaga agatacagct atcgctatcg
     2761 ggcttcgtaa aaatgacaat cgtattagcc aaatcaatgc cagcattgaa accatttcaa
     2821 aagatgacca agttgccttg atggatcgta tgatcaagga acaacctgcc gaagctacaa
     2881 caactgaaga gactagcagt agtttcttta gccaagtcgc taaaattctt tctgaaaact
     2941 ggcaacaact cttgcgtggt gctggtatca ctcttttaat ctctatcgtc ggaaccatca
     3001 taggtctcat tattggactt gccattggtg tcttccgtac tgctcctctc tctgaaaaca
     3061 aagccattta tggccttcaa aaactagtcg gctgggttct caatgtctac attgaaattt
     3121 tccgtggtac gccaatgatt gttcaatcga tggttatcta ctatggaact gcccaagctt
     3181 tcgggatcaa ccttgaccgt acactggctg ctatcttcat cgtttcaatc aataccggtg
     3241 cctacatgac tgaaatcgtc cgtggtggta tcctagcagt tgacaaggga caatttgaag
     3301 ctgcgactgc tcttggtatg acccataacc agaccatgcg taagattgtc ctacctcagg
     3361 tagtccgtaa catcctacct gcaactggta atgaatttgt catcaatatc aaagatacat
     3421 ctgtattgaa cgttatctct gttgtcgaac tttatttctc aggaaatacc gtggcaacac
     3481 aaacctatca atacttccag acatttacaa tcatcgccgt gatttacttt gtcctcacct
     3541 tcaccgtaac acgtatccta cgatttatcg agcgccgaat ggacatggat acctatacta
     3601 caggtgctaa ccaaatgcaa acggaggatt tgaaataatg acacaagcaa tccttgaaat
     3661 taaacacctc aaaaaatcct atggacaaaa cgaagtgcta aaagacattt cactcactgt
     3721 ccacaaggga gaggtcatct ctatcatcgg aagctctgga agcggaaaat cgaccttcct
     3781 acgctccatt aacctacttg aaacaccaac tgatggacaa atcctttatc atggacaaaa
     3841 cgtcctcgaa aaaggctatg acctcacgca ataccgtgaa aagttgggga tggttttcca
     3901 atcctttaac ctctttgaaa atctcaatgt tcttgaaaac acaatcgtcg ctcagacaac
     3961 tgtcctaaaa cgcgaacgca cagaagctga aaagattgcc aaagaaaacc tggaaaaggt
     4021 cggcatggga gaacgctact ggcaagccaa accaaaacaa ctctcaggtg gtcaaaaaca
     4081 acgtgtggcc atcgctcgtg ccctctccat gaatccggac gctattctct ttgatgaacc
     4141 aacatcagct ctcgatccag aaatggttgg agaagtcctc aaaatcatgc aggacctggc
     4201 tcaggaaggc ttgaccatga ttgtcgtaac ccatgaaatg gaatttgccc gtgatgtctc
     4261 tcaccgtgtt atctttatgg ataagggcgt gatcgctgaa gaaggtaaac cagaagacct
     4321 cttcaccaat cctaaagaag accgaacaaa agagttcctt caacgctatc tcaaataaaa
     4381 agaaaaggct gcatcaaccg tgcagtcttt ttgctgtcct caaaatgaaa aggcagattc
     4441 tctatctcta ctacttgaag gtcaagaatc cgccgttatc caaagattaa tcttcaaatt
     4501 tttcataatt tttttcatag aaatcaatta agcctagggc tgtttcaaat gaaatatttt
     4561 ttacttttgc acgaccctgc gccagagcaa tgatagacat ttcacgcgca ttcgtttctt
     4621 tagagatacg gtagcctgtg attttcttat cacgaaccca gccaacaact gattctactt
     4681 tttcaaagtt tgacttagcc atgtttttct cctattttct tctttataat atttaattgt
     4741 atacgttttt actacttttg tcaataaaaa aaacatatat tcaacgaaaa taattagttc
     4801 agaattttct tacttccttt ttaaatccga taatatatag gttttgctag cctctacacc
     4861 ataaatattt gctaataaaa ctactgtatt attaccctct taaggtgcga aattattcaa
     4921 gttcttaaaa aatgcttctc tatttccgta agatagctta tttcttcgat ttaagaactg
     4981 aagatgatag ttctaacctt tagaaatcca agccttcact agctcaaaaa ctcctatctt
     5041 tcttcctcaa taaatcatct gagagatagg tatttcttat atccttcaaa ttcatcactc
     5101 ctttggatat tgaggtgcta gcctagaagc agacacccga ttatctaaac catccttaat
     5161 agagatgaca agacagataa gctcagttaa ttacttatat atctttttaa tatggtatat
     5221 ttgtcaacaa aaagaaatca aatcatacct tatactcaat gaaaatcaaa gagcaaacta
     5281 ggaagctagc cgcaagttgc tcaaaacact gttttgaggt tgcagataga gctaacgtgg
     5341 tttgaagaga ttttcgaaga gtattaaaga cagtgtgact atgcttgtca atagatagga
     5401 aatcaaaatg aaggatggaa atctgagcca aacatcataa gtgctataat gaacagaagt
     5461 aagaattgca cgataagttt tctaaaacca taggaatcgt cccctttctt catcttcatt
     5521 atagcatata tacatcagtt gtacaaataa tatgatattt ttttattagt cctcagacaa
     5581 gcatattata aagcgtttca ttaccattta agttaatata agctggatca aaaccttcca
     5641 ttctacgaat caaacctgca taatcatgct tatctgctaa agcgatccca atcaatactg
     5701 ggcctgttcc cttgctagct cgtttgatat actcaaaacg tgtgatatca tcatttggcc
     5761 ccaggatatc atttacaaac tcacgcaaag ctcctggacg ttgtgggaaa ttgaccacaa
     5821 aataatgttt gataccatca taaatcaagg cacgctcttc catttctggc atacggttga
     5881 tatcattatt tcctccagaa atgatacaac aaatggtttt ccccttaata tattcagcta
     5941 aaacctctaa agaggcgata ctagctgctc cagcaggttc tgcgactatc ccttgcttag
     6001 agtaaaggtc aatcaaggtt tcagaaatca atccctcatc gacacctacc aaagtttgaa
     6061 tatgttgacg agttgcttca taggtcaact gacctacctt ttgcacagca atcccatccg
     6121 caaatttatc aatttccttg agtttgactg gacctccagc ctcaaaggca gctttcatgg
     6181 aacgcgctcc attcgcctct actccgataa cctcaatctc tggacttgtt tccttgatat
     6241 aggtagaaac cccggcaatg agaccgccac caccaacagg aaccaagaca gcatcaaaat
     6301 caatcgattc ttttcgagct tcttctaaaa tctcataagc aactgttcct tgacctgctt
     6361 gaacatgagc atcatcaaaa ggatcaataa aggtacgatt ttcagagact gtaaattctt
     6421 gagctgcttt ggctgaggca tcaaaggtat ctccaactag tttaatagtt acaaaatccc
     6481 caccaaaaaa gcgaacctga ccaatctttt gttgtggcgt agtaatgggc ataaagatag
     6541 tagcaggaat tttcatttca ttacaagtat aggctactcc ctgcgcatga tttcccgcag
     6601 aagcgcaaac taccccacgt tcacgttctt ccttgctgag ctgggaaatg gcataatagg
     6661 caccacgaat tttaaaggag cgaacacgct gggcattttc ttttttcaaa taaatcttag
     6721 caccatactt ctccgataaa taatgatcgt aatccagtgg agtattcaca accacaccgt
     6781 tcaagacctt gtgagccttg attatatctt ttgaacttaa catataaaac ctctttttct
     6841 ataaaaaaca gtcactaatg actgtttttt agttattcat ctccgaaaat ttcagtaaca
     6901 ctttcatggt atttatgagc catacgaatc gaaaactctt taggaagttc ataacgattt
     6961 gtggaaatcc aatcatataa ccattctacc aaactttctt cactatcatt acgatggaag
     7021 ataaaatagt ttaaaatttc tttatattct acatcattca aatccaacgt gtctacaaat
     7081 tgactacgag gaacaaaatt agaatctaat tttcttagtt gatctgtcaa ttctcccaat
     7141 tgtaaatcca actgaaaaaa tagtttttgc agttgcctat cagtttcatc acatcctgtc
     7201 ttgatagaaa acaaattggt aatattcata tgatttcaac ccctaaataa gtacatctaa
     7261 tatcccacga atagctgaag ctaaagtttt cgcagtccag tcatttaaac ccgattcttt
     7321 caatccagat gcaataattt gctctatttg atataaaccg ccatctatcg tctcatttat
     7381 tctgcgaaga ggaccttcaa tataggataa agcttctccg aatctaacag ctgatttagg
     7441 tcctaatgat ttaccaacta tatttgttaa tattttttat ttctcaaaat ccatttcgct
     7501 atataacgag cagatttagt cgcaccagct ctctcaaacg gagaatgaat ttcaagacct
     7561 tcttctctag ctatttgtgc caatttgaag aattgttcaa aggtcatatg caacctacca
     7621 tcttcaaaat tagcatcatc ataaatgtta attacagcat tttcatcata cgaccaatct
     7681 gctttatttt ggtctgctag cactattacc gacggtgtaa ttacattata caactagaac
     7741 caataataac gatgcaattt ttctcataat ctttttcata aaatcatctc cagcgctata
     7801 tatttctaat tagttataga ttttgaatgc atcatcgtcg tttttaccaa cgaatggcat
     7861 tgctttacgc aattctgcac caactttttc aatttcaagg ttagctgctt gttcacggta
     7921 agcagtcaat tttggacgtc cagctttata gtcatttaca aagtcatttg caaatttacc
     7981 attttggatg tctgccaaga cagccttcat attttcttta acttgttcag tgattacacg
     8041 tggacctgat acatagtcac cgtattcagc agtgtttgaa atagattgac gcattttctt
     8101 gaatccacct tcgtagatca agtcaacgat caatttcatt tcgtgaagaa cttcaaagta
     8161 agccaattct ggagcgtaac ctgcttctgt caagacttcg aaacctgctt cgataagggc
     8221 agtcaaacca ccacaaagta cagcttgttc accaaacaaa tcttcttcag tttcttcttt
     8281 gtaagttgtt tcaagaagac ctacacgagc cgctccaaca cctttacacc agtccatagc
     8341 aatgttttta gcatttcctg ttgcatcttg gtatactgca taaagagctg gaacaccaaa
     8401 tccttcttcg taagtacgac gtaccaagtg tcctggtcct ttaggagcac acatgaagac
     8461 atctacatcc gcaggaactt tgataaattc aaagtggatg ttgaaaccat gggcaaatcc
     8521 aactgcgttt ccagcttcca agtttggagc gatttctgct tcgtacaatt cttgttgaat
     8581 ttcgtctggc gccaagatca tgataacatc agccaactta gtagtttctg ctactgtgta
     8641 agtatcaaat ccatcttctt ttgctttatc aaaagattta cctggacgta caccgataat
     8701 aacgtcacga cctgaatcac gcaagttttg agcatgcgca tgcccttgtg aaccataacc
     8761 gataacggcg atttttttac cgtcaagtgc tgctacttta acatcttttt catattccat
     8821 ttgaactgtc atagcttttc tctcttttct attatttatt gccttttaag ctggtttaac
     8881 aaatttaagt tggattttta atcgcgggta aatccagttg cacccgttcg agcaatattg
     8941 cgaataccgt atgggcgaat gactcgcaat agggcttcgc tcttttctgc atttcccgtc
     9001 atctgaatgg taatcgagct tggcgctacg tctaccactg ttgcacggaa aggttgaata
     9061 atcgctaaaa tctcagctct cttctcagct ggcgctgaca tcttaaccaa aatcacctcg
     9121 cgctccaaat gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat ctgacgattg
     9181 agctgtttga tgatttgctc cacttcatca tgagaagcaa catcaataat aatagtgata
     9241 cgcgatacat tcggatcttc tgttgctcca acagagatgc tttcaatatt aacctgacga
     9301 cgagatagga cacctgtaaa gcgattgagg actcctgaac gattttgtag ttttgctgtt
     9361 aacattctac gcatggaact gcacccccaa catctcatga ttactcttac cagccggtac
     9421 cattggtaac acctgttcct tacgagaaat atctacctca attagcatag gaacatcctc
     9481 agtgatgact tcaaggtctt gagccaaggt ctcaggattg tcaaacttat agtttttaat
     9541 accataagcc tgcgccatca attggaaatc aggaagggta tcaaagaccg actctgatgt
     9601 tctgccttca tagaaggatt cctgccactg gcgaaccatt ccgagtgaat gattgttcag
     9661 cataaccacc ttgattggca ccttgtaaat attcaaaata gccaactcct ggttggtcat
     9721 ttggaaacca ccatccccaa caaacaagac tacttcctta tctgggttag caattttagc
     9781 accgattgct gctggaattc caaagcccat tgttcccaaa ccacctgaag tcactaactg
     9841 acgttcattt tggtagggat aatactgagc tgtccacatt tggtgttgac caacgtctgt
     9901 taccacaatg gcatctccat tcgtcaattc accaattcgt tcaataactg cttgcggttg
     9961 aaccacacgc tctttcttgt catacgaacg aacacgattc ttgtctttag tgactttctc
    10021 aatccacttt tcagtattgt tgtgaactgt tggttctgct agcaacattt gcaaggcctt
    10081 cttagcatct ccaactacag gaatgtctgc actgataatc ttgccaatct cagctgggtc
    10141 aatatcaatg tgggcaacct tagcattctt agcgaaagtc ttaggattcc ccgtcaaacg
    10201 gtcatcgaaa cgagaaccaa tactaatcat aaagtccgct tccgtcatgg caatatttgc
    10261 tgcgaatgac ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg ttgcaatcgt
    10321 tccttgtccc aaaagactgg ttaccactgg aatttgatag cgttctgcaa attcatttag
    10381 ttccgtagca gcctcagcat aactaattcc accaccagct aacaagactg gctttttagc
    10441 cttggacaat tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa gagtcggctg
    10501 ataacttggt aaattcactt ctggtgaata aatgaagtct gtttctaaag cagatatgtc
    10561 ttttggtagg tcaattacaa ctggccctgg acggcctgta gttgcgatat ggacagcttc
    10621 cgtaatgata cgcggaatat cagctgtctc acgaacttgg taattgtact tagtgattgg
    10681 catggtaatt cctacgatgt ctgcctcctg aaaggcatcc ttcccaatcc ctgctcgcgc
    10741 cacctgacct gtaaagacca aaaggggaac gctatcgctc atagcatccg caatccctgt
    10801 aatggcattt gttgctcctg gtccactagt gacgacggca acacccaact ttccagttga
    10861 tttggcatag ccttcagctt catgcaaaca accttgctca tggcgcccta gaatgtggcg
    10921 aatgccttta aaattatata tcgcatcata aaaaggcaaa accgcaccac caggataacc
    10981 aaagatggta tcaactccta aatcacgaag tgtttccaaa actaggtccg accccgtctt
    11041 aggagattct aaactgattt tctccattat tcccctttct ttcctcttaa aaataacttg
    11101 ttactatcat accatttttt caaaattttt caagataaaa gaagaaattt tctgaatttt
    11161 ctattttaac gtttatttat gaatgttatt tttgttttta ttaaaaagat accgatttca
    11221 tttactaaaa agaaaaaaga actgatttct cagtccttca ttaatcttat tccacactaa
    11281 ataggtatgg gtaaacaggt tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt
    11341 cttctacgat ttcttgagcg atttcattgg caagttcttc gcttccgtct tcaccgacat
    11401 agaaggttac gatttcactg tcttcatcca acatatgttt caaggtttca gtcaatgttt
    11461 ggtgcatatc agggtttgac acaagaattt ttccatccac catacctaaa ttatcgtttt
    11521 catggatttc taagccatcg atcgttgtat cacgcacggc tgttgtgacg cttccgctaa
    11581 cgacatcgct aagagcagct gtcatacgct cttggttttc ttcaatggac ttgcttggat
    11641 caaaggcaag aagacttgtc ataccttgag gaagagtgcg agcctctacc actaccgctg
    11701 gttgctccaa aacttctgcc gcagattgag ctgccatgaa gatgttcttg ttgtttggca
    11761 agaagatgat gttacgggca ttaacctgtt caacagcctt gataaagtct tctgttgaag
    11821 ggttcatggt ttgaccgcct tcgataacat aatccacgcc ttgagaacag aagatatctg
    11881 ctagaccttt accagccacc acagcaatca aagcatactc tttttcttca gccgacttga
    11941 taacttgagt agcttctttc tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt
    12001 ttaccttgac caagctacca tatttgagac cttcttgcat aacaagtcct ggatcttctg
    12061 tatgaacatg gactttgaca atttcatcat cgttaacaac gaggagagaa tctccaagct
    12121 catccaagta gttacggaat tcatcgtagt caaaatcttt agcataggtt ggaccttgct
    12181 taagagctac catgatttca gtacagtaac caaacgtgat gtcctcagtc gctacgtgac
    12241 cagctacaga cttatgatgc tctacattga tcatctcact catgttggca ggagtcgcta
    12301 caaagtcctc agatgcaata tattcgccag taagggctga aaggaaacct tcgtagatga
    12361 agaccaatcc ttgaccacct gagtccacaa cgccaacttc tttcaatact ggaagcatgt
    12421 ctggtgtttt agctagagct gttttagcac cttccaaggc tgcgcgcatg acttcaacag
    12481 tgtcatctgt ttgctcagct tttttcttag caccgatagc agctccacga gaaactgtca
    12541 aaatcgttcc ttcaacaggt ttcatcactg ccttataggc aacttccaca cctgattgga
    12601 aggccagagc caagtcttga cctgttaact cgtctttatc cttgatagct tgggaaaatc
    12661 cacggaaaag ctgagacgta atcactcctg agttcccacg cgcacccatc aaaagccctt
    12721 tggcaagaat gctcgctact tctccaactg tagaagctgg cttgtctgca acttctttag
    12781 caccattttc aatggtcatt cccatatttg tcccagtatc tccatctgga actggaaaga
    12841 cgtttaatga attgacatat tcagcttgct tattcaagcg agttgatgca gcctgcacca
    12901 tttcttgaaa taagctagta gtaatttttg acacggttat tctcctacaa ctttgatatt
    12961 ttgaatgtag acatttacag tctgagcagt aattccaagc tggttttcca agctaaaggc
    13021 aacacgctct tgaatgtttt ttgacacttc actaatcttt gttccgtagc ttaacacggt
    13081 atatacatca actgcaatac tgccatcttc ggctgccttt acgacgacac ctttagaata
    13141 attttcctta cctagcaggg cttggaaatt atctttgagg gcatttttac tagccatacc
    13201 gaccacacca aaaatctcag ttgctgctcc acctacgacg gttgcaatca cttcatctgt
    13261 tagttcgatt tgaccatctt ttgtattaat ttttacagtc atccttttta cctcaactag
    13321 ttgatactct attttatcat atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg
    13381 atatttactc tatttttcaa acgatgttat acccacaata aaagaaaaaa gaccctaagg
    13441 tctcctttgc ttttattatt aaacgcgttc aactttacct gatttcaaag cacgagctga
    13501 agcccaaact tttttaggtt taccatcgat aagaacagta actttttgaa ggtttggttt
    13561 tacggcacgt tttgtttggt tcatcgcgtg tgaacggttg tttcctgata cagtcttacg
    13621 acctgtaaag taacatactt tagccattgt gttttcctcc tattagatct aatatagcgg
    13681 atgtgctagc accacatacc gtactatgtt atcacacttt cttgtttttt gcaagggaat
    13741 tggaagattt tttatttgtg tcttaaatca ggtcttgcgt gacattttct gctctccaca
    13801 tgccattgtt gattaacaga acaccagaat taaaattatg tgtataaaaa tcatctctaa
    13861 ctgcagctaa gggtatagcc gtcaagtcca aatcccacag ctcatctatc gattttctta
    13921 caacaatatc tgaatccaaa tacagtacac gagactcgct tacatacttt ggaataaaat
    13981 acctaaaaaa agccgcatat gaaagtccct caaaggggag acgataacct ttcagaatac
    14041 tactgtcaat ctgaacattc acaatctcac tattcaaagt ctctagtctt ttttccatca
    14101 attggaacca ttctcgcgga aggtcatcat taaaaacata aaacttaaga ttataatgat
    14161 gaacacaaag agattttatt gttgtttcaa ctttatccat ataagcatta tctgcaccta
    14221 agacaatcgc ttttttctct tctttcactc tttatcttat ttctttttat tcccatcata
    14281 ttattcccat catatgtttc tacgtaacca ttattttcgc ctattcgttc gtaaaaccat
    14341 accagtggag attttagatg aagtcccatt acggtttaca atttttacat tacgacacgg
    14401 agttttacaa atcgatttca tttgccaaac gtagttagtg aggcagttag ctagttcgcc
    14461 aaatagcgac tagcgtccaa caatttggaa ctttagctcc aattgttggt actgagtcac
    14521 atcttctcct ctaactctac gtctggatac ttgtccgcaa accagcggag ggcaaagtca
    14581 ttttcaaaga gaaagactgg ttggtcaaaa cggtctttgg ctaagatatt gcgacttgac
    14641 gacatccgtt catccaagtc ctcaggcttg atccaacgaa cggtcttttt acccattggg
    14701 ctcatgacca cttccgcatt gtattcgcct tccatacggt gtttaaagac ttcaaactgg
    14761 agttgaccaa cagctcccag catgtactca cctgtttggt aattcttata aagctgaacg
    14821 gctccttctt gcaccaattg ctcaatcccc ttgtggaagg atttttgctt cataacattc
    14881 ttggcagaaa ctttcatgaa aatttcagga gtaaaggttg gcagtggttc aaattcaaac
    14941 ttgttttttc caaccgtcaa ggtatcccca acctgataag taccggtatc gtaaaccccg
    15001 ataatatcac ctgccacggc attgatcaca ttctcacgac tttccgccat aaactgggta
    15061 acattagata gtttagcccc cttaccagta cgagggagat tgacactcat gccgcgctca
    15121 aattcgcctg atacgatacg gacaaaggca atacggtcac ggtgacgagg atccatgttg
    15181 gcttggattt taaagacaaa gcctgagaaa tccttgtcat aaggatccac aatttcaccg
    15241 tctgttttct tgtgaccatg tggttctgga gcaaacttga ggaagatttc aaggaaggtc
    15301 tgcacaccaa agtttgtcag ggctgaaccg aaaaagacag gcgtcaattc tccagccaga
    15361 atagcttcct ctgaaaactc attcccagct tcatttaaaa gctcaatgtc atccttgact
    15421 tgctcgtaga aaggattgct accaaagagt ttgtccccgt cttctagact ggcaaaacgc
    15481 tcatcccctt tgtaaagctc taaacgttgg ttatagaggt catacaagcc ctcaaaggct
    15541 ttccccatcc cgataggcca gttcataggg tagctagcaa tgcccaagat ttcttccaat
    15601 tcttgcaaga gatccaaagg ctcacgaccg tcacggtcca gcttgttcat aaaggtaaaa
    15661 actggaatgc cacgatgttt cacaacctca aacaatttct tggtttgagc ctcgatcccc
    15721 ttggcagagt ccacgaccat gaccgcagca tccaccgcca tcaaggtacg ataggtatct
    15781 tctgagaagt cctcgtgccc tggcgtgtct aagatattca cgcgcttgcc gtcgtagtca
    15841 aattgcataa cagatgaagt aacagaaatc ccacgttgct tctcgatatc catccagtca
    15901 gatttagcaa aagtccctgt tttcttccct tttaccgtac cagcctcacg aatctcaccc
    15961 ccaaagtaga gtaactgctc agtgatggtt gttttccccg cgtccgggtg ggagataatg
    16021 gcaaaggtac gacgtttctt aatttcttct tgaatattca taagttctct ttctttgatt
    16081 ctctattttt cttgtttcaa tagctgagaa tgatttttac attggatttt accattcctt
    16141 tcaatactcc attatatcgg attttagcat ttttttcaat ttctatctct tttcacttcc
    16201 ccctccctta tttataggaa aatatggtaa aatagaacag actaaaaatc atcatttcac
    16261 gaaaggatgc aagatgaaaa ttacgcaaga agaggtaaca cacgttgcca atctttcaaa
    16321 attaagattc tctgaagaag aaactgctgc ctttgcgacc accttgtcta agattgttga
    16381 catggttgaa ttgctgggcg aagttgacac aactggtgtc gcacctacta cgactatggc
    16441 tgaccgcaag actgtactcc gccctgatgt ggccgaagaa ggaacagacc gtgatcgctt
    16501 gtttaaaaac gtacctgaac aagacaacta ctatatcaag gtgccagcta tcctagacga
    16561 tggaggagat gcctgatgac ttttaacaat aaaactattg aagagttgca caatctcctt
    16621 gtctctaagg aaatttctgc aacagaattg acccaagcaa cacttgaaaa tatcaagtct
    16681 cgtgaggaag ccctcaattc atttgtcacc atcgctgagg agcaagctct tgttcaagct
    16741 aaagccattg atgaagctgg aattgatgct gacaatgtcc tttcaggaat tccacttgct
    16801 gttaaggata acatctctac agacggtatt ctcacaactg ctgcctcaaa aatgctctac
    16861 aactatgagc caatctttga tgcgacagct gttgccaatg caaaaaccaa gggcatgatt
    16921 gtcgttggaa agaccaacat ggacgaattt gctatgggtg gttcaggtga aacttcacac
    16981 tacggagcaa ctaaaaacgc ttggaaccac agcaaggttc ctggtgggtc atcaagtggt
    17041 tctgccgcag ctgtagcctc aggacaagtt cgcttgtcac ttggttctga tactggtggt
    17101 tccatccgcc aacctgctgc cttcaacgga atcgttggtc tcaaaccaac ctacggaaca
    17161 gtttcacgtt tcggtctcat tgcctttggt agctcattag accagattgg accttttgct
    17221 cctactgtta aggaaaatgc cctcttgctc aacgctattg ccagcgaaga tgctaaagac
    17281 tctacttctg ctcctgtccg catcgccgac tttacttcaa aaatcggcca agacatcaag
    17341 ggtatgaaaa tcgctttgcc taaggaatac ctaggcgaag gaattgatcc agaggttaag
    17401 gaaacaatct taaacgcggc caaacacttt gaaaaattgg gtgctatcgt cgaagaagtc
    17461 agccttcctc actctaaata cggtgttgcc gtttattaca tcatcgcttc atcagaagct
    17521 tcatcaaact tgcaacgctt cgacggtatc cgttacggct atcgcgcaga agatgcaacc
    17581 aaccttgatg aaatctatgt aaacagccga agccaaggtt ttggtgaaga ggtaaaacgt
    17641 cgtatcatgc tgggtacttt cagtctttca tcaggttact atgatgccta ctacaaaaag
    17701 gctggtcaag tccgtaccct catcattcaa gatttcgaaa aagtcttcgc ggattacgat
    17761 ttgattttgg gtccaactgc tccaagtgtt gcctatgact tggattctct caaccatgac
    17821 ccagttgcca tgtacttagc cgacctattg accatacctg taaacttggc aggactgcct
    17881 ggaatttcga ttcctgctgg attctctcaa ggtctacctg tcggactcca attgattggt
    17941 cccaagtact ctgaggaaac catttaccaa gctgctgctg cttttgaagc aacaacagac
    18001 taccacaaac aacaacccgt gatttttgga ggtgacaact aatgaacttt gaaacagtca
    18061 tcggacttga agtccacgta gagctcaaca ccaattcaaa aatcttctca cctacttctg
    18121 cccactttgg aaatgaccaa aatgccaaca ctaacgtgat tgactggtct ttcccaggag
    18181 ttctaccagt tctcaataaa ggggttgttg atgccggtat caaggctgct cttgccctca
    18241 acatggacat ccacaaaaag atgcactttg accgcaagaa ctacttctat cctgataacc
    18301 ccaaagccta ccaaatttct cagtttgatg aaccaatcgg atataatggc tggattgaag
    18361 tcaaactaga agacggtacg accaagaaaa tcggtatcga acgtgcccac ctagaggaag
    18421 acgctggtaa aaacacccat ggtacagatg gctactctta tgttgacctc aaccgccaag
    18481 gggttccctt gattgagatt gtatctgagg cagatatgcg ttctcctgaa gaagcctatg
    18541 cttatctgac agccctcaag gaagttatcc agtacgctgg catttctgac gttaagatgg
    18601 aggaaggttc gatgcgtgtg gatgccaaca tctcccttcg tccttatggt caagagaaat
    18661 tcggtaccaa gactgaattg aagaacctca actccttctc aaacgttcgt aaaggtcttg
    18721 aatacgaagt ccaacgccag gctgaaattc ttcgctcagg tggtcaaatc cgccaagaaa
    18781 cacgccgtta cgatgaagcg aataaagcaa ccatcctcat gcgtgtcaag gaaggggctg
    18841 ctgactaccg ctacttccca gaaccagacc tacccctctt tgaaatttct gacgagtgga
    18901 ttgaggaaat gcggactgag ttgccagagt ttccaaaaga acgtcgtgcg cgttatgtat
    18961 ctgaccttgg tttatcagac tacgatgcta gtcagttgac tgctaataaa gtcacttctg
    19021 acttctttga aaaagctgtt gccctaggtg gtgatgccaa acaagtctct aactggctcc
    19081 aaggggaagt cgctcagttc ttgaatgctg aaggtaaaac actggaacaa atcgaattga
    19141 caccagaaaa cttggttgaa atgattgcca tcatcgaaga cggtactatt tcatctaaga
    19201 ttgccaagaa agtctttgtc catctagcta aaaatggcgg tggcgcgcgt gaatacgtgg
    19261 aaaaagcagg tatggttcaa atttcagatc cagctatctt gatcccaatc atccaccaag
    19321 tctttgccga taacgaagct gctgttgccg acttcaagtc aggcaaacgt aacgccgaca
    19381 aagcctttac aggattcctt atgaaggcaa ccaaaggcca agccaaccca caagttgccc
    19441 ttaaactact tgcacaggaa ttggcgaagt tgaaagaaaa ctagacagaa caaaaccagc
    19501 cctaaggttg gttttttctt ctctaccaac tcccaataac tattttggct ttatttccag
    19561 agtattttat ggtaaaatga agagtaataa tatttattaa agaggtaaaa acatgattga
    19621 agcaagtaaa ttaaaagctg gtatgacctt tgaaacagct gacggcaaat tgattcgcgt
    19681 tttggaagct agtcaccaca aaccaggtaa aggaaacacg atcatgcgta tgaaattgcg
    19741 tgatgtccgt actggttcta catttgacac aagctaccgt ccagaggaaa aatttgaaca
    19801 agctattatc gagactgtcc cagctcaata cttgtacaaa atggatgaca cagcatactt
    19861 catgaataca gaaacttatg accaatacga aatccctgta gtcaatgttg aaaacgaatt
    19921 gctttacatc cttgaaaact ctgatgtgaa aatccaattc tacggaactg aagtgatcgg
    19981 tgtcaccgtt cctactactg ttgagttgac agttgctgaa actcaaccat ctatcaaagg
    20041 tgctactgtt acaggttctg gtaaaccagc aacgatggaa actggacttg tcgtaaacgt
    20101 tccagacttc atcgaagcag gacaaaaact cgttatcaac actgcagaag gaacttacgt
    20161 ttctcgtgcc taatctctag aaagaggtca ttctatggga attgaagaac aacttggcga
    20221 aatcgttatc gccccacgtg tacttgaaaa aatcattgct atcgctactg caaaggtaga
    20281 gggtgttcac tctttttcaa acagatcagt gtctgatacc ctttcaaaac tttcactcgg
    20341 ccgtggcatt tatcttaaaa acgtggacga agaactcaca gcagatatct atctctacct
    20401 tgagtacgga gtaaaagttc ctaaggtagc ggttgctatc cagaaagctg tcaaagatgc
    20461 cgtccgtaat atggctgatg tagaactcgc tgctatcaat attcacgttg caggtatcgt
    20521 cccagataaa acaccaaaac cagaattgaa agatctattt gacgaggact tcctcaatga
    20581 ctagtccact attagaatct agacgccaac tccgtaaatg cgcttttcaa gctctcatga
    20641 gccttgagtt cggtacggat gtcgaaactg cttgtcgttt cgcctatact catgatcgtg
    20701 aagatacggc tgtacaactt ccagcctttt tgatagacct cgtttctggt gttcaagcta
    20761 aaaaggaaga actagataag caaatcactc agcatttaaa agcaggttgg accattgaac
    20821 gcttaacgct cgtggagaga aacctccttc gcttgggagt ctttgaaatc acttcatttg
    20881 acactcctca gctggttgct gttaatgaag ctatcgagct tgcaaaggac ttctccgatc
    20941 aaaaatctgc ccgttttatc aatggactgc tcagccagtt tgtaacagaa gaacaataaa
    21001 aagaaaaagt gtttgatatc tatcaaacac tttttctttg cctcccacta tctaaaaaaa
    21061 tgataataga tataattgta aacaaaaatc cagataggtt ttgcatgatt gagaaagtta
    21121 aaaaaactat ggcagagaat cgttaatctc agattgtcgg tagaacgata aacaagggca
    21181 aaaaagaaac caatcagact ataatataat aaactaattg gatctctgtg agatagtatc
    21241 aaatgactaa gcccaaagat aaggcctgat aagatgacat ctaaataata cttggactag
    21301 ggaaagaagg tattcataaa ataccctcta tcaagagtct cctcaaaaac aggaccgatg
    21361 attacaggca ggacaaaaga taagatagtc gataaaaagg ttggttgtcc atttgaaaga
    21421 accacggtaa aatactcatc atgaatattc ctatgattaa tcaaatgagc atagcgtgcc
    21481 caaaaattac cgagaatctg ataaaccaca taagttgcaa ataaggagaa gacaaatgac
    21541 cagttccagc tctttttctt ttttaacctc caaattaata gaaaaggaaa cttcccacta
    21601 atcccactgt taaaataaga gaatagacat cagctcctaa ccctaaaatg atcgtcacat
    21661 acaatccaat tgtttgtggt aaataggtag atagtaaaat aataagcaaa aatattccaa
    21721 attgtcttag tttctttgtg tttctcatcg tacttttttg aaagattacc ctgttcggaa
    21781 gccgtacttc caagcatcta tataagaatt aagtgcccct tgcctcatat agggagcaaa
    21841 ttctctataa tataaccatc tactatatcc atcttcccaa acagcaagac cacctgaagt
    21901 ttgctccaag tcctcagttg aaataactgt aaatgtttct gtacctgtca ttgcaagtac
    21961 cttcttaaaa tagattgttg taggctcaca tttatagtat atttcttttt ttgtctattt
    22021 tatagtctat ctcctcaact ggcaattttt cgacctgaat tgcatttttc cataaaaaat
    22081 gagacctttc tagtctcatt tagtcattct tagtattttc taaatcgttg atagcgttct
    22141 tccagcaact cttctagcgg tttttgtgaa agtctagcca gctccgtttg gagttctttt
    22201 ttgacactct taattagttc tttactagaa agtcctactt cagaaatcac cttatccacc
    22261 acgtccattt ctaacagttc atgcgaagtg attttcatca gttctgctgc ttccatggcg
    22321 cgagtaccgt ccttccataa aatggaagca aagccttctg gactgagaat ggcatagata
    22381 gaattttcca gcatccagac acggtccgcg acagctagag ccagagcccc gcctgaacca
    22441 ccttcaccga taataatggc gataatagga actttcaggt cactcatttc catgagattg
    22501 cgagcgatag cttccccttg accacgttct tccgctccga caccaggata agcacctgct
    22561 gtattgataa aggtcacaac tggacggcca aatttctcag cctgtttcat caaccgcagt
    22621 gcctttcggt agccttctgg atgtggttgg ccaaaattcc gtttgaggtt gtcttgcaaa
    22681 ctcttgcctt tttggatacc aaccactgtt acagcttggt ctccaagcca accaatacca
    22741 ccaacaactg caccatcatc acgaaaagaa cggtcaccat gtaattggat aaattcatca
    22801 aaaatgcctg tcgcaaagtc caaggttgtc aagcgactct gctcacgcgc ttctctgact
    22861 atttttgcaa tattcatcta ggactccctc catgcaatct gactaggcta gcaatcgtat
    22921 ctggtaagtc tcttcttttg acaatagcat ccacaaagcc atgttctaat aggaattctg
    22981 ccttttggaa atcctcaggc aagctttcac gaaccgtatt ttcaatcaca cgacgcccag
    23041 caaaaccaac caagctctgt ggttcagcca gaatgatatc gccttccata gcgaaagaag
    23101 ctgtcacacc accagtcgtt ggatctgtca aaatggtcag gtaaaagaga ccagcatttg
    23161 aatggcgttt aaccgctgca gagatcttag ccatctgcat gagactcatg attccttcct
    23221 gcatacgggc tccaccagag gctgtgaata ggacaactgg caatttttcg acagtcgcat
    23281 actcaaacaa acgagtgatt ttttcaccta caaccgtacc catagaagcc atgataaaat
    23341 tagaatccat aatcccaaga gccacagtct gacctttaat aagagcagtt cctgtcacaa
    23401 cggcttcatg cagacctgtt ttttcacgca tagatgccag tttcttttgg taaccaggga
    23461 aatgcaaggg atccttgctt tcaatccctg taaacaattc tttgaaggtt cccatatcaa
    23521 tcgtcaaagc caagcgttct tgggcagaaa tacgaaaggt atagctacag tgcggacaga
    23581 tacgttcact tcccagatcc ttctgataga tggtatgctt acagcctgga cactgggaaa
    23641 ataattcatc tggaacctct ggcttagctt gaggtttttc cctaaccgaa cgattgggat
    23701 tgattcgaat atacttatct tttttactaa atagagccat tgattcccct tttcggttta
    23761 aactcttaaa gtcattttat tctttttctt gatatttagg taagaaggtt tccatcaaga
    23821 aggaagtatc ataatcccca gcaatgacat tgcgatctga aatgagatca agctggaaat
    23881 ctgcattggt ctgcactcct tcaatctcta attcatagag ggcacgttgc attttcatca
    23941 aggcgtcaaa acgattttcg ccgtgtacta tgattttggc aatcatacta tcataataag
    24001 gcggaatggt ataacctgga taaactgctg aatccacgcg caagccaact ccaccacttg
    24061 gcagatagag attagtaatc ttacctggac ttggagcaaa gttaaaggct gggttttctg
    24121 cattgatacg acactcgatg gcatgaccgc gtaggacaat atcttcttgc ttaacagaca
    24181 aaggctgacc tgccgcaatg caaatctgtt ccttaacgat atcaacacct gaaacaaact
    24241 ctgttactgg atgttctacc tgaacacgag tattcatctc catgaaatag aaatttctac
    24301 ttgcttcatc aagaagaaat tcaatggttc ctgcattctc atagccaaca aactctgccg
    24361 ctcgaacagc agcagcacct atttcatgac gcagcgtttt tccgattgca atcgagggac
    24421 tttcttccaa aaccttttgg ttattccttt gaagagaaca atcccgttca cccaagtgaa
    24481 tcacatgtcc atgctcatca cctaggattt gaacctcaat gtgccgagct ggatagataa
    24541 cccgttctat gtacatggca ccattgccat aattggcctt ggcctcacta gaggcagttt
    24601 caaaggcaga aacgaggtca tctggttttt caaccttacg aatcccttta ccacctccac
    24661 ctgctgaagc cttgagcata acaggatagc caattttttc agcaacaatc aaagcttctt
    24721 cagagttatg cacttctcca tctgaacctg gtataacagg cacacctgct ttaatcatct
    24781 gagcacgcgc attgatctta tcccccatca tatccataac atgaccagat ggaccgataa
    24841 acttgatacc tacttcttca cacatggtcg caaatttgga attttcactg agaaatccaa
    24901 aaccagggtg aatagcttct gcctcagtca agactgcagc tgatagaact gcattaatat
    24961 tgagataaga ctctgttgcc ttgccaggac caatacaaac tgcttcatct gccaaaagcg
    25021 tatgaagagc ttccttatca gcagttgaat aaaccgctac cgtcgcaatc cccaattcac
    25081 gtgccgcacg gataatacga accgcaattt caccacgatt ggcaattaaa atttttcgaa
    25141 acatggagaa cctccttagt tcccaattgc aaaagtaagg ataccactgg ctgcaagctt
    25201 gccatccact tcagcctttg cttcaaccac agctatggtg ccacgacgtt ttacaaaagt
    25261 cgctgtcata accaattggt cgcctggtac aacttgcttc ttgaacttaa ccttgtccat
    25321 accagcgtaa aagaccagtt ttcctttatt ttcaggtttt gataactcca acacaccagc
    25381 agtttgcgcc aaggcttcca taatcagaac acctggcata actgggtatt gaggaaagtg
    25441 gccgttaaag aaaggttcgt taatggtcac atttttgata gcaacaatgg tatcctcgct
    25501 cacttccaag acacggtcca ctagaagcat aggataacgg tggggaagag cttctttgat
    25561 tccttgaata tcgatcattt gatacgtacc aatcctttac caaactcaac catttcttcg
    25621 ttagagacga gaatttccgt taccacacca tccttaggag ctgggatttc attcatgact
    25681 ttcatggctt cgataattac caatgtttga ccttttttga cactatcacc aactgtaacg
    25741 aaggcaggtt tatctggtcc agcagccaag taaaccactc caacaagtgg actctctaca
    25801 agatttccct cagtagccac acttgcttca gctggagctg gaacttcttc tgctacagtc
    25861 tctgctggag cagatgtagg agctactgga ctcggtgttg ctagaacggg tgctggagcg
    25921 acttgagttg caacttcagg cacaggtctt gcttcattct tgctaaactg caactcatcc
    25981 gtcccatttt tataagaaaa ttctctcaaa cttgactggt caaattgagt catcaagtct
    26041 ttaatatcgt ttaaattcat acttatctat tctcccaacg tttgaaagca agaactgcat
    26101 tgtggcctcc aaaaccaaaa gtatttgaaa tagcgtatgg aatttctttc tccaaacctt
    26161 gtccataaac gacattagct tcgatataat ctgatacttc acttgtccca gctgtcattg
    26221 gtacaaagtt atgacgcata gcttcgatgg tgacgatagc ttctactgca cccgcagccc
    26281 ccagcaaatg tcctgtaaaa gacttggttg atgatacagg tacttcctta ccaagaacag
    26341 ctacgatagc accactttct cctttttcat tggcaggagt tgacgttccg tgagcattaa
    26401 cataggctac ttgctctgga gaaatctcag cttcttccaa ggctagtttg atggccttga
    26461 tagctccctg accttctgga tgtggagaag tcatgtggta ggcatcacaa gtatttccgt
    26521 aaccaaccac ttcagccagg atagtagctc cacgtttttc agcgtgttca agactttcta
    26581 gaaccaacat ccctgaacct tcacccataa caaacccatt gcgatcctta tcaaatggga
    26641 tcgaagcacg agttggatcc tctgtagtag agagagctgt taaggcttgg aaaccagcga
    26701 tggcaaaagg tgtgatagaa gcttctgttc ctcccaccaa catcacatct tggaaaccaa
    26761 acttaatgga gcggaaggca tccccaatcg catcatttga tgaagagcag gcagtattga
    26821 tagatttaca aacaccgttt gcaccaaaac gcatggctac attcccagaa gccatatttg
    26881 gtaaagcttt tggaagagtc attggtttga cacgtttggg tcctttttca tgaaggcgaa
    26941 gtacctgatc ttcaatttcc ttgattccac caataccaga tgcaacgata acaccaaaac
    27001 gatccctatt aagagcctct acatcaagat tggcatggtt tacagcctct tgggctgcat
    27061 acaaggcata taaagaatag ttatcaaaac ggttggtatc tttttttaca aagtatttat
    27121 cgaacggaaa atcttggatt tctgccgcat tatgcacatc aaagtcacta tgatcaaatt
    27181 ttgtaatgcc accaatgccg attttcccag ttgctaaact attccaaaat tcttctggtg
    27241 tatttccgat tggagatgtt actccataac ctgttaccac tactcgattt agtttcattc
    27301 ttttcacctc tagctttcgc tacatactta agccaccatc aatggcaatc acttgaccag
    27361 ttagataatc ttggcctgct aaaaatactg tcaaatctgc aacctgctct gcctgcccaa
    27421 attctttcat cggaatctga gctagtgtag cttccttaat cttatctgat aagatagctg
    27481 tcatatcaga ctcaatcatt cctggagcaa tcacattgac tcgtatattc cgactagcga
    27541 cctcgcgtgc cacagacttg gtaaagccaa tcaagccagc cttagaagca gcatagttag
    27601 cttgaccaat attccccatc aaaccaacaa cactagacat attaatgata gcaccttctc
    27661 tggctttcat catcggtttc aagactgatt gtgtcatatt aaaggcacca gtcagattga
    27721 ccttgagcac tttttcaaaa tctgcttctg tcatcttgag cataagagta tcttgggtaa
    27781 tccctgcatt gttgaccaaa acatctactg aacccagttc tgcaatagct tgatcaatca
    27841 tacgcttagc gtctgcaaaa tctgatacat ctcctgaaat gggaaccacc ttgataccat
    27901 agtttgaaaa ctcagcgagc aattcttctg agattgcccc acgactgttt aagacaatgt
    27961 tggctcctgc ttgagcaaac ttgtgggcga tggcaagacc aattccacga ctcgaacctg
    28021 taataaagat atttttatgt tctagtttca tttttttcct ttcaaaactt ctacttattt
    28081 tagtctattt ttctaaaagt gctactaaac tcgcttgatc ttccacatga gctaagtgag
    28141 tagtttgatc aattttttta acaaaacctg acaagacttt ccccggtcca atctcgataa
    28201 agttgcttat gcctgcttct tgcatgaccc caatactttc atagaaacga acgggttcct
    28261 tgacctgacg cgtcaagagc tgagcaatgt cctctttttg catcacagca gcttctgtat
    28321 tgccgactag gggacaagta aaatctgaaa aacttacctg agccagagtt tcagctagtt
    28381 tctggctagc aggttcaagg agagaggtgt gaaagggacc tgacacctta agaggaatca
    28441 agcgtttggc acctgcttct tgcaaaagtt caaccgctcg atcaactgca accacttctc
    28501 cagcaatgac gatttgtgca ggtgtgttat agttggctgg agtaaccact ccaagttcag
    28561 aagctttttg acaggcttct tcaatgacct ctactggcgt attgagaact gctaccatct
    28621 tgccagagtc agcaggagcc gcttcttcca tataggctcc acgcttagct accaaggcaa
    28681 ccgcatcttc aaaatccaag gcgccgcttg ccaccaaggc agagtattct ccaagagaca
    28741 aaccagcaac catatcaggc tgatagccct tttcttgcaa taaacggtag atagcaaccg
    28801 aagtcgctag aatggctggt tgcgtatagc gggtctgatt gagtttgtct tcttccgtat
    28861 cgatgagata acgcaaatca taacctagca cctgactcgc tcgatcaatc gtttctttaa
    28921 caatcggata ctgatcatag aaatcccgtc ccatccctag atactgggca ccttgaccag
    28981 caaataaaaa ggctgtttta gtcatttctt acaactcctg tccagcgaga ggcttcttct
    29041 tgaattttct tagcggctcc gtaatacaaa tcttttagga tttcttcagc tgtttcttct
    29101 ttagaaacaa gccctgcgat ttgacctgcc atgacagagc caccctccac atcaccgtga
    29161 acaactgctt tggctagggc acctgctccc atttgttcaa agatttctaa atcaggatct
    29221 tcctgcttaa aggcatcttt ttcagccagt tcaaaatctc tagtcaactg atttttaata
    29281 gcacgaacag catgaccaaa gtgctgagct gaaatcgtag tgtcaatatc ccttgctttt
    29341 aaaattttct ccttgtagtt tggatgggca ttcgactctt ttgcaactac aaaccgtgtc
    29401 cccacctgta cagcctctgc acctagcata aagccagccg cagcaccttc accatccgca
    29461 attcctcctg cagcaataac aggaatagat acagctgtgg ctacctgtcg caccaaggtc
    29521 atggttgtta atttaccgat atgcccccca gcttccattc cttctgcaat aacagcgtct
    29581 gcaccgattt tttccatgcg tttagctaaa gcgacactag gaacgacagg aataacgatt
    29641 atcccagctt catggaaacg ttccatatac ttgcttggat ttcctgctcc tgttgtgaca
    29701 actttaacac cttcttcaat aacgagatcc acgatatctt ccacaaaggg agataagagc
    29761 atgatgttga ccccaaaggg tttatcagtc aatgatttga ttttatcaat attggccttg
    29821 acaacttctt tcggggcatt tcccccaccg ataattccta atcctccagc cttggaaaca
    29881 gcccctgcca aatcaccatc agcaacccag gccatccctc cttggaaaat aggatagtca
    29941 atcttcaata attctgtaat acgcgttttc atagtgcctc caaccttcct tgcttacgta
    30001 atagttcgat ttcaccataa tttgacagtc aaactattac ctaaacaaga gggagtgggt
    30061 ttctccctac tccttctact aatattctgc ttattttgct tgctcttcaa cgtaagcaac
    30121 caagtcacca actgttttca agtcattttc tgcttcgatt tggatatcaa aagcatcttc
    30181 gatttctgag attacttgga acaagtccaa tgaatctgcg tccaaatcat caaaagttga
    30241 ttcaagtgtt acttctgatg cgtcttttcc aagttcttca acgataattt cttgtacttt
    30301 ttcaaatact gccatgatag gactccttta aaataaatag tttttttata acaatgtgtt
    30361 caccacatga ttacctaaat tgtaagaatg agcgtgcccc aggtcaaacc tccaccgaag
    30421 cctgatagaa gaacagtctg gctaccatct aaagggatga gaccttgttc tacacactct
    30481 gaaagtaaaa tcgggatact ggctgcactg gtattgccat attccatcat attggctgga
    30541 agtttggctc ggtcaacacc aatttttcta gccatcttat ccaaaatacg gtcattggct
    30601 tgatgaagta gcagataatc caagtctgtc acctctatag gagattcatc aatagtctgc
    30661 ttgatagact tggctacatc tcgaatggca aaatcaaaga ctgtgcgtcc atccatcttc
    30721 aaaaacgaat ctgcactttc ttgatctgaa aatggagaat gcaaacctga atgcccataa
    30781 gttaaacact cgctgcgact tccatcgcta ttgagactct cagctaagaa atgctcttgc
    30841 tcgctagctt ctaacaagac accaccagca ccatctccaa acaacacagc tgttgatcga
    30901 tccgaccaat cgactgcctt agagagggtt tcactaccaa tcaccaagcc tttttgaaag
    30961 cgaccagaag cgataaactt ttcagcagtt gaaagagcaa atacaaatcc actgcaagcc
    31021 gcggttaagt caaaagcaaa ggctttatta gcaccaatat tagcttgaac acgagcagct
    31081 gtagagggca tcatcgaatc tggagtaatg gtagctagga tgataaaatc cagttcttct
    31141 cctgttattc cagcttttgc catcagtttc ttagcaacct ctgtagccaa atcactggta
    31201 gattctgttc ttgaaatatg cctttgtcgt attcccgttc gacttgaaat ccactcatca
    31261 ttggtatcca taatctgagc caagtcgtga tttgtaacca cttgctctgg cacataatga
    31321 gcaacctgac ttatttttgc aaaagccatt atttcaaatc ctccaaaaat tggtaaagat
    31381 tagtcaagcc tttacccatg acagcaattt cttcctcgct catgccatca ataatttttt
    31441 ctaccatggc cttgtggaag cgtttatgca gtctatgaat caagcgaccc ttctttgtca
    31501 aatgcagatg caccacacga cgatcctgtt ctgaccgaac tcgctcaatg tagcccttac
    31561 gctctaaatt gttcaaactt gtcgtaacag ttccaagagt taccattaac tcttttgaca
    31621 cttgacttgg agtcacatct ggagccttac cgatgacatc aatcgtatgc atttctttga
    31681 tggagatatc cttaaaacga ctacctctca agttcacttc ctcaattaca aggacattgt
    31741 taaatataga tgttaaatat tcattgattc gttggtagtc catttttcat atccctcctt
    31801 cttcaaaaaa cttttacaat caaaacattt gacagaaaga attatatcaa aattcaaaga
    31861 atgatgcaag tattttttat tttcctgtaa atttaggtct tcttctctcc gaatgagctc
    31921 gcactccttc tttgaaatcc tctgtttgag ctaaggattt ctgtaggttc agttctaaag
    31981 tagcgtaacc ctgccagtct ttaaattggc tctcccaaac caacttctta atggcagcat
    32041 aggaattact tgaagcacgt cttaattttt taagaagctg ttctctcgtc ttttcaagtt
    32101 tttcagcttc agaaacgcgg taaaccaacc cccattctag ggctttttct gccgttaaag
    32161 cctctcctgt catagctaat tgagcagcac gcgtcacacc gatactgcgg ctcaagagat
    32221 gaattccccc tgcatctgga gccaaaccaa caccaacaaa ggcttggata aacttagcct
    32281 tatccgtcgc caaacagaaa tctgccgcaa cagccatatt cgctgcggca cctgcaacag
    32341 caccgtcaac ttccattaaa acaggtttgg ctatttgctt gattttataa gaaatcgtat
    32401 tgaccaactc tgcgattttt gtcaatgatg gaatatcatc ctcatccact gcccgcttca
    32461 tctctaccaa atctccccca actgagaaga cctttccatt ggcattgatt aagataaaat
    32521 gcacagctgg attttcttct gccaaagtca gagcttctaa aatctcctca cacatgggaa
    32581 tatgaaaacc atttgcgacc tcaggacggt tcaaggtaag gattgccaag tcctcttcaa
    32641 gctgataaat aatgtgttcc atcagggctc ctttttattt tataagcagt ttatactctt
    32701 cgaaaatctc ttcaaaccgc gtcagcttcc atctgcaacc tcaaaacagt attttgagct
    32761 gacttcgtca gtcttattta caacctcaaa gcagtgcttt gagcagtctg cggctagctt
    32821 cctagtttgc tctttgattt cattgagtgt taaattattt cactgtcaaa gtatatcttt
    32881 tttaggagca gaaagcaaga aaaaatcgac tgaaagtgtc tcagccgata agaaaggctc
    32941 gctttaggct agaaagcatc ttattttctt cattgaaaaa agcctttctt tctgctataa
    33001 tcgtataaaa tacttacttt aggagttctt atgaaagttg ttaaatttgg aggtagttct
    33061 cttgcctctg ctagtcaatt agaaaaagtt ttaaacatcg tcaaaagcga ttcagagcgt
    33121 cgttttgtag tcgtttctgc gcctggtaaa cgcaatgctg aagatactaa ggttacggat
    33181 gccctgatta aatactaccg cgactatgtt gcgggtaacg atattagcaa gagccaaagc
    33241 tggattatcg accgctatgc tgctatggtt agtgaattgg gactaaaacc agctgtgcta
    33301 gaaaaaattt ctaaaagcat tcacgccttg gccactcttc ctattgaaga aaatgaattt
    33361 ctctacgata ctttcctagc agccggtgaa aataacaatg ccaaattgat tgctgcctac
    33421 tttaaccaaa atggtatcga tgcacgctat atgcacccta gagaagctgg gattgtggtc
    33481 acaagtgaac ctggtcacgc tcgcatcatt ccatcaagtt atgacaagat tgaagaattg
    33541 acaaacacca atgaagtcct tgtcattcct ggtttctttg gtgtcactaa ggaaaatcaa
    33601 atctgtactt tctcacgtgg aggttctgat attacaggtt ctatcattgc tgctggtgtc
    33661 aaagccgacc tctatgaaaa ctttacggac gttgatggta tctttgcagc ccaccctggt
    33721 attatccacc aaccacactc gattcctgag ttgacctacc gtgaaatgcg cgagttggcc
    33781 tatgcaggct tctcagtcct tcatgacgag gctcttcttc ctgcctaccg tggaaaaatt
    33841 cctctggtta tcaagaatac caacaaccct gaccatccag gtactcgtat cgttctaaaa
    33901 cacagtaatg atgaatttcc agttgtggga attgctggtg actcaggctt tgtcagcatt
    33961 aacatgtcga aatacctcat gaaccgtgag gttggatttg gccgcaaggt tctgcaaatc
    34021 ctggaagaac ttaacatcgg ttgggaacat atgccaacag gtatcgacga tctttctatc
    34081 attctccgtt ctcgccaact aactcccatc aaggaagaag aaatcctgcg tcagttggtt
    34141 caaaaggcta aagtagatca tgcagaaatc gaacacgacc tttctatcat tatgattgtt
    34201 ggtgaaaaaa tgaagagcca catcggagtg actgctactg cgacacgcgc tctatctgaa
    34261 aacaaaatca acatccagat gatgtctcaa ggttctagtg aagtttctat tatgtttgtt
    34321 gtcaataaag accaagagaa agcagcaatt aaagccctct acaatgcctt ttttggagaa
    34381 agtaaggaag actaagcatg gcccaatcac ttaacaaaac agtactcctc agtacgacag
    34441 gcacttccta cctctctatc gctgggaaag ttgggaaatt ccttgtcgga gatcaggctt
    34501 tggaatttta cccagatgtc aatgtcgaac aatttatcca gattccttgg agccatatca
    34561 atcagattgg agccaatgtc actggacgca aaatcagccg ccacttcgaa gtctttacag
    34621 acaaaggaaa attcctcttt gcttcaaaag actcaggtgc cattctcaaa attgcacgcg
    34681 aaaaactagg caatgacaag gtcgtcaaac ttccgaccct gattcagaca attagccaaa
    34741 aatttaaaaa tctatttgca aaaaagtaga aactttagta taatcatagc aacggataag
    34801 taggttatct tcttctttca gaaagtctgc gggtactgcg agcagatagg ggggataggt
    34861 gaaattctac cgttagtaac aattacatac acaatcaagt gcacacctgt gaagttggat
    34921 ggaaccgtgg ccctgccact ccaacgtttt gtcaggcgtg cttttttcat aaaggagttc
    34981 ttatgttaga tatcaaacgt attcgtacag attttgaagc tgtcgcagaa aaattagcta
    35041 cacgtggtgt agaggctgct gtcttgaatg aaatgaaaga aatcgatgct aaacgtcgta
    35101 acatcttggt caaggttgaa actctcaaag cagaacgtaa cacagtttct gctgagattg
    35161 cccaagctaa gcgcaacaag gaaaatacag atgacaagat tgctgccatg caaaatctat
    35221 ctgctgaggt taaagccttg gatgctgaat tggcagaaat cgatgctaaa ttgacagaat
    35281 ttacaacgac tcttccaaat atcccagctg acagcgttcc tgttgggact gacgaagacg
    35341 acaatgtgga agttcgccgt tggggtactc cacgcgagtt tgacttcgaa cctaaagctc
    35401 actgggatct cggtgaagac cttggtatcc ttgactggga acgcggtggt aaggtaacag
    35461 gcgctcgctt cctcttctat aaaggcctcg gtgctcgttt ggaacgtgct atctacaact
    35521 ttatgttgga tgaacatgga aaagaaggct atactgaagt catcacacct tacatagtca
    35581 accatgattc tatgtttggt actggtcagt atccaaaatt taaggaagat acttttgaac
    35641 tcagcgatac caactttgtc ttgattccaa ctgctgaagt tcctctgaca aactactacc
    35701 gtgatgaaat cttagacggc aaagatcttc caatctactt cactgccatg agtccgtcat
    35761 tccgttctga ggctggttct gccggtcgtg atacgcgtgg cttgatccgt ttgcaccaat
    35821 tccacaaggt tgaaatggtc aaatttgcca aaccagaaga atcttacgaa gaattggaaa
    35881 aaatgacagc caacgctgaa aacattcttc aaaaactcaa ccttccatac cgtgtcgttg
    35941 ctctctctac tggagatatg ggcttctcag ctgcgaagac ttacgacttg gaagtgtgga
    36001 ttccagcaca aaacaattac cgtgaaatct caagctgttc aaacacagaa gatttccaag
    36061 cccgtcgtgc ccaaatccgt taccgtgatg aagcagatgg caaggtgaaa ctccttcata
    36121 ccttgaacgg ttctggactt gcagttggac gtacagtggc tgcaattctt gaaaattacc
    36181 aaaatgaaga tggttctgtg accatcccag aagcacttcg tccatacatg ggtggagctg
    36241 aagtcatcaa accataaaaa ataaggttta gctatttcta gctagacctt ttttcgtaac
    36301 caaatcagat aagcacctag tacaaagaat aaaatagtta ggcatataat ggtttcagtc
    36361 aataccaggt aatccagaaa tggaagtttc aaaattccct gagccatctt gagcgaggtc
    36421 gctgtgataa tggttgggaa ggtgagggct gagaaggctg gttgaaaacc ttgttttaaa
    36481 atgttgggca gacgagttaa aacaaagaaa aagaaggatt gagaagccaa aatcatgaca
    36541 atcaagaccc aagtcggcag gctggttcct cctactcgaa ctagagaagc caagagtaga
    36601 gagaaaggag cacagtagat tccttcttgt ccaagcaagg ctagtgggag tggatgtttc
    36661 tttaaatcgc tataaataag gggatagaga tagaaggtca agagaaaacc aaaactcaag
    36721 gtcgcatagg caatttcgat aatacctacc agaggatagg tcaaggcagc cactgctatc
    36781 cccacataga gaaccgtcca gcttggagtg gcatgaaccc tccgccctgg acaagcaaac
    36841 ttgatggtaa aaccagcaat caaggtcaaa tccaagagaa atgaaaacca ccaaatccct
    36901 tgtgctacca aaggaagata agagaatacg cgaaagacat aggtcgataa aatcatccca
    36961 gccataggaa aggttgccat tcctgacaaa agagggggct tggtcaattc ttgcttggtt
    37021 tctttccaat taaagagatg cagaattaga aagtaaatcc ataaaaccaa accagtcaga
    37081 ctaaaaagat gggatagaac cggcaacgta tctaaaataa gatttccagc tcctgccaaa
    37141 cctagcaaac aacctgaaaa tactaagggg agttttttca tcctaacctc caataatcat
    37201 gttagtttca gtataacata aaagcgctta aatgaggatt taaaaaaacg aagtccgctt
    37261 atttcagact tcattttact cagatatgaa ttaggcataa ggttgcaatt ctggattaat
    37321 tggtgtatta gctaagttgt tggcatagtt acagaggatt gctaggctga caccaaaaac
    37381 cacatccaag gcattttgtt gagtgtagcc agcttctaaa aactcagaca aggcttcatc
    37441 tcctacacga cccttggtat tgataactgc caaggtaaac ttagctaggg tatccaattt
    37501 aggatctgtt tcaattggag tacgattgcg aagagcttga atcaagtcat cattcatctg
    37561 gatttgtttg atggaaaagg ctgtgtgacc tgcgacacag aaggcacaac cattggtcac
    37621 ggctgccgtg atttgcacca cttcacgctc aacgggtgtc aggctgttgc gacggtggat
    37681 agatgagaca atttggtagg cttctaaaac agtcggggca ttggccaaga gaccgattag
    37741 gttgggaata tagccattgt tgtctttttc tactgtttca agaatttctt tcacttctgc
    37801 tggtgctgac tctactgtat ggatagtaaa tgttgtcata agatacctct tttcttatta
    37861 ttgacactaa tattattgga aaatcttata aaatcctgat tcctaagttt atctaagata
    37921 aagctttatt ctctcataag attttcgttg ttatattagt ttatcacact tccaatcact
    37981 tgcataatat atattatata tcaggctgat aaaaattatt tataggcaaa aaaatcacac
    38041 gagctgtgtg attccattat ttgtcaaaat actttttagt ttcagcaata acgactggcg
    38101 acaagaccaa gagggcaatc aagtttggca gagccatcaa ggcgttaacg atatctgcga
    38161 taatccagac catatccaac tcgataaatc ctcctaacaa gaccatgagc acaaaaaaca
    38221 cacggtagag ccagataaag cgagccccaa agaggaactc aaaacagcgt tctccgtaat
    38281 agttccaacc tagaatcgtt gtaaaggtaa aaagtacaag gaagatggtc aagagggcag
    38341 gcccaaagtg tgaaaagact gttgagaaag ctgactgagt caaggcaacc ccattcaagt
    38401 caccactcca aactccagtt accaagatgg tcaaaccagt tagagtacaa atgatgaggg
    38461 tatcaataaa ggttcctgtc atggaaatca aaccttgctc tactggttca tttgtcttgg
    38521 cagctgcagc tgcaatagga gcagaaccca gaccagattc gtttgagaac acaccacgcg
    38581 ccacaccatt ttgaatagcc atccgaacgc tagcaccagc aaatccacct accgcagcaa
    38641 ggggactaaa agctgaggta aagactaaag cgattgtgcc agggattttt ccgatattaa
    38701 agaaaataac tgtaagagtt cctaagatat aaatgagggc cataaaagga acaacagtag
    38761 ttgaaacctt agaaatagac ttgagtccac caaagactgc aatcgctaca aagacagaca
    38821 agacgagagc tgtgatggct ggcgaaatcg tcgttgtatt ttggatagat tctgtaatcg
    38881 agttgacttg ggtgaaggtt ccgattccca agagagcaac caatactcct gctactgcaa
    38941 acaaaacagc aagtggtcgc cacttttctc ccatccctag aaggatatag tgcatgggac
    39001 ctcccgctac tgcaccatgg tcgtccttgg tgcggtattt gatggccaag agtccttccg
    39061 catacttggt agccattcca aagaaagccg ccatccacat ccaaaataga gctcctggtc
    39121 caccaacctt gatagccgtc gcaactccta tgatatttcc tgttccaaca gttgatgcca
    39181 aggctgtaca cagagctgca aaactggata catcaccatg tcccttatcc tggataaaaa
    39241 taagctgaaa ggccttgggc agacgcaaaa cctgcaagag tcctagccgc atggttaggt
    39301 aaatccctgt tccgaccaat aaaatcaaga ggggcggtcc ccaagcaaaa gcatcgattg
    39361 atttaagcaa ttctaacatt ttcttctcct atcgtttcaa ccccaaaaga aagagcacat
    39421 gcaagataca tgtactctgg aatgcttaga taaatgctaa aaagcggtct atcctagctc
    39481 tgtcctttta cctgagagtt tgagcagttg cctgccttgc cccttcggtg cctttacggt
    39541 ctctccagag ttccgtccat ttacagtcat ggaaaatcaa acgattcccc acttctatta
    39601 aacttcattc ggtgttggta tttaattgat tctaatttta caaaaaatgt tggcttttgt
    39661 caatgtgttt attagtaaaa attagttcaa cagtttttac tttataaagt ccagaatact
    39721 gctatccttt aaaagtgaca atagtcgcac cactgcctcc agcattttgt ggggcatagc
    39781 cgaaactctt gacatgtttg tttctttgca agtatttggt aactccttca cggatgactc
    39841 ctgttccgat accatggatg atatcaactt gagccatatt gttaagcaag gcttggtcga
    39901 tgaaggtatc tagctcattc atggcttctt catagcgctt gcctcgaaga tccagtctag
    39961 cttgaggtcc tcgcccagaa gttcgtttca caacattgac ctgtttcttc ttgactggtt
    40021 tttcttgctg ggcttgaaca agatcaaact ctttctcttc caaggtcatc ttaatcaagc
    40081 caatttgggc ttcccagcgg ccgtccttga gttgactggt caaggtacca cgctgaccat
    40141 aactgagaac cacgatatca tctcccacct ttggagctcg ttttttcttg gccttttgaa
    40201 ggaccttgtt tttagacaag tccacttttt caggagccaa ttttttcaac ttggccttgg
    40261 cttcaatgat ttcgtggggc ttgagttggg atttactgtg gagatttttg agaatctggt
    40321 cactttcact tagggccata tccacaatct cagcagcctg ttcacgcgcc ttgttaagct
    40381 cggtttcctt ttcacgatta agctcgttgt agagtttttt tagcgcacgg ttcatcttga
    40441 gattttcttg ctccacctca cggatattgt ccaagcgttt gcggctttcc agcgtctgct
    40501 cttctaattg ctcaatgata cgattgacgt cattgtcctg atcgatctgc tgactggcat
    40561 ctcctacgat aacttcagat aggcctagac gtttggcaat ttcaaaggca ttacttcggc
    40621 caggaacacc ctgcataaag cgataggtcg ggcgaagagt tgcagtatca aactccatac
    40681 tggcattttg cacaaaggct gtctcaatac cgtaggcctt gagttctgga tagtgggtcg
    40741 tcgccatggt cttgatttga cgcaggcgaa ggtcctccag aatagccatg gcaagggctg
    40801 ctccctcttg gggatcagta ccagccccca actcatccaa aagtaagagt gaatgttggt
    40861 tgaccttgcc aagaatatcc acgatattgg tcatatggct agagaaggta gacaagctct
    40921 gctcaataga ctgctcatct ccaatatcag caaagatttc ttcaaaaata ccaacacgac
    40981 ttcccttgtc tgctaaaatc ggcaatcctg actgggccat gacctgtgtc aagcccagag
    41041 ttttaagcat gatggtcttc ccacctgtat tgggacctgt aatgacaata gctgttaaat
    41101 cttgaccaaa atagacatca tttgcgacgg catttttgac caaaggatgg cagacatgga
    41161 gcagttgaat ctcttgattt tctgacagct gaggcacgac tgcttgtctt tcttgaataa
    41221 atcgaacctt ggcacgaatc aagtccagat gaccgataat ccaagcgtca ttagcaatct
    41281 cagccgcatg agggcggaca cgctcagaaa tttcttggag aatgcgaagc atttcatagc
    41341 gctcatctgc tcgcagacta gcaatttctt cgctcagttt gactacctca cgtggttcga
    41401 tatagacggt gtttccacta gcagaaatat catgaacgac acctgcaatc ttattgcggt
    41461 aggtgttttt gactggtaaa acctgacggc catttctgct agcaacaatt ccttccgtca
    41521 acatctgccc tttttgcttg agcaagtctt gtaaaacatc gcgtacctga ctctcgctat
    41581 catgtatttt tcgacggatt cgcgccaatt cttcactggc aaaattttca atgaaacccg
    41641 catcattaaa ggcctgaaga tttccttgta attgcggaaa atcatgtaat ttctcaaacc
    41701 aaagggctaa ttcttccaag ctgacatttt ccagattggc gtaaaaactt tgaagttctc
    41761 ggctggcaag aagcacgcgt ttcaagagta ggaactcctc gatattgaga tccgctccca
    41821 tctccaacct cttgcagact cctgcaattt ccttagttga gagaatagta aaatgcggtt
    41881 gctcgacgaa aagagcctgc atttctttca tctcagcaaa agcctgtttg attttatctg
    41941 ctttggcagt cggagccagc tgtctcaatt gctccaagcc ctgctcggtc aacaaatgag
    42001 gctcaaacaa ggccttgacc ttatcgaact ctaatgtttc taatattttc ttattcatct
    42061 ttatttcctt gtctttgaat gtctaggtgt ggtagctttt tacattctga tagattcgag
    42121 tcaaatctgt aaacaaaggg ctaggaaaac tcctgccctt tttatccgat taaatttgtc
    42181 acccagattt gtttgagcca actggttgtt atcggtatgc tctggatgat gtgttttgcg
    42241 acgatactct tttcaagagg attttgtata actgccatag ggatggtcgc caagattgtc
    42301 aaggccattt gcaagacaaa taaggtcacc aacatggaca agatacctgc tgaaacttgg
    42361 aacaacttac cacccagttt tttgctagga atcaagtgta agagaagacc aagcaaacga
    42421 ccaatgctat agacaatccc aaatacaagc aagtagccga tacctgcata aaagacctta
    42481 tccagctgaa agagttgatc cgatgggaaa aagaaagtcc cctgaccttc ctgcgaattt
    42541 gcataaggga gcaataaatg gaattgctct ccaagcccct tataaaactg gccagccata
    42601 aaagccgatg ccatggctga aatcaggtaa taaacctgta agagcaggcc tctccgatag
    42661 ccgatataaa atccccaaac caagaccaat agaagaagga atgaaatcat aaggaatcct
    42721 caatcttgct ctgttcttgc ttacaagtca caagcttgtg acggagttct tctagctctt
    42781 gctccttatc gtcaaattca atctcacggc tgagctgagt tgataaacag ttgactgcca
    42841 acaaaagagc gattgtttca tcatctgcgc taggcatttg ttctttaatt gcttggtatt
    42901 tttctgtcgc aaccttagcg atttcctcca taaaaaggtt atcatgttcg cttgtcaagg
    42961 ttaacgtttt tttcccgaat gtaaatttga atcgatttag atttgccata aaattcacct
    43021 cacgatatta taccaaaatt cgctaatttt gtcagttttt acaaatttta ctgcttttgt
    43081 ggtacaatag aaactatggc aagtatcaca ctcacaccaa gcgaaaagga tattcaggct
    43141 tttcttgaac actatcaaac cagtctggct cctagcaaga atccctatat ccgctacttt
    43201 ttgaaactac ctcaagcaac ggtttctatc tatacttctg gaaaaatctt gcttcagggt
    43261 gaaggggctg aaaaatacgc cagtttcttt ggctatcaag ctgtagagca aaccagcgga
    43321 caaaatcttc ctttaattgg gacagatgag gtgggaaatg gttcctactt tggtgggctt
    43381 gcagttgtgg ctgcctttgt cacacctgac cagcacgact ttttacgaaa actcggtgtg
    43441 ggggattcta agactctgac cgaccaaaag atccgtcaga ttgctcctat tctcaaggaa
    43501 aaaattcagc accaggcact ccttctctca cccagcaagt acaacgaggt catcggagac
    43561 cgctacaatg ctgtttcggt taaggttgcc ctccataatc aggctatcta tctcctcctt
    43621 caaaaaggtg ttcagcctga gaaaattgtg attgatgcct ttaccagtgc taaaaattat
    43681 gacaagtact tggcacaaga ggccaatcgt ttcagcaatc ctatcagctt agaagaaaag
    43741 gctgagggca aatacttggc tgtcgcagtt tcttctgtca ttgcgcgtga tctctttctg
    43801 gaaaatcttg aaaatctggg acgagaactg ggttatcagc tcccaagtgg agctggaaca
    43861 gcttctgaca aggtggctag ccagattttg caagcctatg gtatgcaggg actcagcttc
    43921 tgcgccaaat tgcactttaa aaacactgaa aaagcgaaaa aacgcttaga aaggtaagtt
    43981 atgaattcat ttaaaaattt cttaaaagag tggggattgt tcctcctaat tctgtcatta
    44041 ctagctttaa gtcgtatctt tttttggagc aatgttcgcg tagaaggaca ttccatggat
    44101 ccgaccctag cggatggcga aattctcttc gttgtaaaac accttcctat tgaccgtttt
    44161 gatatcgtgg tggcccatga ggaagatggc aataaggaca tcgtcaagcg cgtgattgga
    44221 atgcctggcg acaccattcg ttacgaaaat gataaactct acatcaatga caaagaaacg
    44281 gacgagcctt atctagcaga ctatatcaaa cgcttcaagg atgacaaact ccaaagcact
    44341 tactcaggca agggctttga aggaaataaa ggaactttct ttagaagtat cgctcaaaaa
    44401 gcccaagcct tcacagttga tgtcaactac aacaccaact ttagctttac tgttccagaa
    44461 ggagaatacc ttctcctcgg agatgaccgc ttggtttcga gcgacagccg ccacgtaggt
    44521 accttcaaag caaaagatat cacaggggaa gctaaattcc gcttctggcc aatcacccgt
    44581 atcggaacat tttaagaaac ctaagaggcc gagaatcacc aatctcagcc tcttcttcta
    44641 tcgtgagaag tgattgttta ttgacaaata ctcaatgaaa atcaaagaac aaactaggaa
    44701 gctagccgta ggctgcttaa aacagtgttt tgaggttgca gatggaagct gacgtggttt
    44761 gaagatattt tcgaagagta taaaataaaa acaatcccaa ctctcaccta ttcatgcaaa
    44821 ggaattttat ggaagtttat ttttcaggaa ccattgaacg gattattttt gaaaatccca
    44881 gcaattttta tcgcatcctc ctcctagaaa tcgacgatac ggacgcagag gattttgatg
    44941 actttgaaat cattgtcaca ggaaccatgg ctgatgtaat tgagggcgaa gactatactt
    45001 tttgggggca aattgtccag cactccaagt atggagaaca actgcaaatc agtcgttatg
    45061 atcgcgcaaa accaactagt aagggcttgg tcaagtactt ttcaagtagc catttcaagg
    45121 ggattggtct caagacagct cagaaaatcg tggataccta tggcgaaaat accattgacg
    45181 aaattttgca acacccagaa aagttagaag gcatcgcagg actctctgcc aaaaatcgcg
    45241 aggctttcgt ctccactctc cgtctcaact acggtacgga gatggttttg gccaaactag
    45301 ccaactacgg cattcccaac aaactagcct ttcagattca agacttttac aaggaagaaa
    45361 cccttgatgt ggttgaaaat tatccctacc aactggttga ggatatcaag ggtttgggct
    45421 ttaccattgc tgaccaacta gctgaggaac taggcatcga aagtcaggct cctgaacgct
    45481 tccgcgccgg tctagttcac agtctttttc aggcctgtat ggaaacagga gacacctatg
    45541 ttgaagcacg ggatttgctg gaacaaaccc ttactctcct tgagtcttcc cgtcccgtgg
    45601 aactagaccc cagccaagtt gcccaagagc tctcctacct gatcgaagaa gacaaggttc
    45661 agcagattga taccaagatc tttgacaaca gcctcttttt cgctgaggaa ggcatccgca
    45721 gtcacttggt tcgtatcctt gaaaaaggaa aacagaagag tcatgattta gaaaccattc
    45781 aaaaacatat cactactatc gaggaagagc tgggaattga gtatgataac attcaaaaac
    45841 aggctatctg tgacgctatc cagaacaagg tctttatcct gacaggtggg cctggtactg
    45901 gtaagacaac tgttatcaat ggaatcatcg ctgtttatgc ccttttagaa ggacttgact
    45961 tcaggaagaa aaacaatctg cccattcttc ttgctgctcc aactggacga gccgctcgcc
    46021 gcatgaatga attgacaggt ttgcctagcg cgaccataca ccgccacttg ggaatgacag
    46081 gtgacgatga taccagtcat ctggaagatt acctagatgc tgactttatc atcgtggatg
    46141 aattctctat ggtggatact tggctggcca atcaactctt ctccaatatc tcttctaaca
    46201 gtaagatcct catcgtgggt gacagtgatc agctaccttc tgtcagtcct ggacaggttc
    46261 tagcggatct gcttcatatt cctttgattc ctcagactcg cttggaaaaa atttatcgac
    46321 aaagcgaaga atcaaccatc gtcaccctag ctagtcagat tcgacagggc atcttgccag
    46381 ctgatttcac ccaaaaaaaa gctgaccgtt cctactttga aattgctagt ggccatattc
    46441 ctgccactat tgaaaaaatc ttaggtgccg ccctcagaaa tagtattcct gcccgtgata
    46501 tccaagttct ggctcctatg taccgaggga cggcagggat tgatgctatc aatcagctca
    46561 tgcaagacct gctcaatccc ccacaaaaag atcaactcag ttttgaagct cctcagtgcc
    46621 actatcgtaa gggagacaag gtcattcatt tggttaacga tgctgaaatc aatgtcttta
    46681 atggggattt aggagctatc acagacctga ttcctggtaa atacaccgaa tcgaaacaag
    46741 acgagattgt cattgatttt gatggtaatg aagtctctta cccccgtaac gaatggtaca
    46801 agattcgctt ggcctatgcc atgagtattc ataagtctca gggaagtgag ttcccagttg
    46861 tcatcctacc gattactagt gctagccgac gtatgctgga gcgaaatctc atctacacag
    46921 ccattacacg tgccaaaagc aaactcatct tactaggcga attacaagcc ttcgactatg
    46981 ctacccaaca catcggaact gcccgaaaaa cctatctgat tgaacgcttc agtgatctac
    47041 tggagaatgt tgaagaaaag caacaagctg tctctgaaac agtcacatca agtgcctctg
    47101 aacaatccta catcctaacc gaagaaaact gggacagcat tccagccatg attgggatta
    47161 cagacacaga cctcaaagag atttttggaa aatagtgcat cagaaaaacc accaattcag
    47221 gtgggttttc tgtttattaa gattatttta ctgttgctgt gcttgtgatc aattcaacag
    47281 cttttttgat agtgatatca tgtttcaaca tgtcagctga aagcaagttt tgaacttgtg
    47341 caacttccat gttgtagtct gctgccaatt gctcaacttc tttttggatt tcttcttctg
    47401 aagcatcaaa tccttcagct ttggcaactg cttcgataac aaggttagtc ttagtacgtg
    47461 actcagcttc tgcttggtat tggttgtgaa ggtcttcttg agtagttcca gtgatttgga
    47521 agtacatgtc agggttgatc ccttgacgtt gcaaattccc aaggaattca tttactgaac
    47581 ggtgaacttc ttcatggatc atttcttctg gaagttctac gatttcagca ttttctacag
    47641 ctgtatcaat tgctgcacct tcaactgcat ctttgtaagc ttcttcttta gcagcagcca
    47701 attctttgcg gtatttttct ttcaagtcag caagtgtttc aacttcttca tcaatgtctt
    47761 ttgcaagttc atcgtcaaga gctggaactt ctttagcttt tacttcgtgg atagttgtca
    47821 cgaatttagc ttctttacct gcaaggtctt ctgcttggta gtcttctggg aatgttacga
    47881 taacatcaac ggtttcgcca gctgagtgac ctaccaattg gtcttcgaaa ccagggatga
    47941 attgacctga accaagtcca agtgagaagt tttcaccttt tccaccgtca aattcaacac
    48001 cgtcgataga accaacgaag tcgatcacaa cagtgtcgcc gttttcagca gcagcttcct
    48061 tgataaccaa ttcagccagg ttgttgcgtt cgcgttcgat acgctcttcg acatcagcgt
    48121 cagttacttc tttttctaca tcaactgata cttcaaggtt tttgtagtca cccaatttta
    48181 cttcaggttt tgtaacgact tcagcagtga taacccagtc ttgacctttt tccattgaag
    48241 ttacgtcaat ttttggttgg gcaaccactt caagaccagc ttcttttaca gctgcttcat
    48301 aagcgtttgg caaaagagcg ttcataacgt cttggtaaag tgattcttca ccaaattttt
    48361 ggtcgaagat aggacgtgga aggtgacctt tacggaaacc tggaacatta agagatttct
    48421 tcactgactt gaagacacgg tccaattctg gtttgatttg gtcttgagag atagtgaaag
    48481 tcaagacacc acggtttgtt tctttgtttt caaatgatac agacattctg tcatttctcc
    48541 ttaaaatttt ttaaatacag tctattataa cattttctat aaataaaatc tagactaatg
    48601 cagccaatta tagacaacaa gcctttttcc taactaaaac actcataaag tatagacaat
    48661 tttagactac tttggatatc ttttggttag tcattttcca aaactaattt atctaatttt
    48721 tcagttatat agtagattga aac
//
LOCUS       DBEJAQ010000008        45884 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.008, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000008 DBEJAQ010000000
VERSION     DBEJAQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 45884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.008"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            2..2023
                     /locus_tag="ACL297_000525"
                     /pseudo
     CDS             2..2023
                     /locus_tag="ACL297_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            2025..2666
                     /locus_tag="ACL297_000526"
     CDS             2025..2666
                     /locus_tag="ACL297_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070836.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            2770..4122
                     /gene="murD"
                     /locus_tag="ACL297_000527"
     CDS             2770..4122
                     /gene="murD"
                     /locus_tag="ACL297_000527"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGR0070837.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4126..5184
                     /locus_tag="ACL297_000528"
     CDS             4126..5184
                     /locus_tag="ACL297_000528"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HGR0070838.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5194..6393
                     /gene="divIB"
                     /locus_tag="ACL297_000529"
     CDS             5194..6393
                     /gene="divIB"
                     /locus_tag="ACL297_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HGR0070839.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            6626..7327
                     /gene="pyrF"
                     /locus_tag="ACL297_000530"
     CDS             6626..7327
                     /gene="pyrF"
                     /locus_tag="ACL297_000530"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGR0070840.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            7361..7993
                     /gene="pyrE"
                     /locus_tag="ACL297_000531"
     CDS             7361..7993
                     /gene="pyrE"
                     /locus_tag="ACL297_000531"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGR0070841.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            8277..8576
                     /locus_tag="ACL297_000532"
     CDS             8277..8576
                     /locus_tag="ACL297_000532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070842.1"
                     /translation="MPRWGSDTTSELEKENASAGINNNDSTGGGKRLNTSIRSAYSGS
                     DITPVYSLGSGSRIVMYYNGGGDNYIGSGTRLAMAPQFGNHVRIHTSGSWNPDSY"
     gene            8672..9436
                     /locus_tag="ACL297_000533"
     CDS             8672..9436
                     /locus_tag="ACL297_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070843.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            9436..10188
                     /locus_tag="ACL297_000534"
     CDS             9436..10188
                     /locus_tag="ACL297_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070844.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            10198..10896
                     /locus_tag="ACL297_000535"
     CDS             10198..10896
                     /locus_tag="ACL297_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070845.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            10893..11540
                     /locus_tag="ACL297_000536"
     CDS             10893..11540
                     /locus_tag="ACL297_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0070846.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(11620..12481)
                     /locus_tag="ACL297_000537"
                     /pseudo
     CDS             complement(11620..12481)
                     /locus_tag="ACL297_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(12493..13251)
                     /locus_tag="ACL297_000538"
     CDS             complement(12493..13251)
                     /locus_tag="ACL297_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGR0070847.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(13252..13929)
                     /locus_tag="ACL297_000539"
     CDS             complement(13252..13929)
                     /locus_tag="ACL297_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGR0070848.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(13889..14557)
                     /locus_tag="ACL297_000540"
     CDS             complement(13889..14557)
                     /locus_tag="ACL297_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGR0070849.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            14783..14917
                     /locus_tag="ACL297_000541"
                     /pseudo
     CDS             14783..14917
                     /locus_tag="ACL297_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            15057..16547
                     /gene="lysS"
                     /locus_tag="ACL297_000542"
     CDS             15057..16547
                     /gene="lysS"
                     /locus_tag="ACL297_000542"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGR0070850.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTVNSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            17172..18308
                     /gene="lctO"
                     /locus_tag="ACL297_000543"
     CDS             17172..18308
                     /gene="lctO"
                     /locus_tag="ACL297_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HGR0070851.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDKRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      18562..18650
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18865..19533
                     /locus_tag="ACL297_000544"
     CDS             18865..19533
                     /locus_tag="ACL297_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HGR0070852.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLIMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            19770..20552
                     /gene="thiM"
                     /locus_tag="ACL297_000545"
     CDS             19770..20552
                     /gene="thiM"
                     /locus_tag="ACL297_000545"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGR0070853.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPE
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            20554..21183
                     /gene="thiE"
                     /locus_tag="ACL297_000546"
     CDS             20554..21183
                     /gene="thiE"
                     /locus_tag="ACL297_000546"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGR0070854.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      21392..21480
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            21742..22302
                     /locus_tag="ACL297_000547"
     CDS             21742..22302
                     /locus_tag="ACL297_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGR0070855.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            22303..23688
                     /locus_tag="ACL297_000548"
     CDS             22303..23688
                     /locus_tag="ACL297_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0070856.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            23690..24340
                     /locus_tag="ACL297_000549"
     CDS             23690..24340
                     /locus_tag="ACL297_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGR0070857.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            24351..25043
                     /gene="tenA"
                     /locus_tag="ACL297_000550"
     CDS             24351..25043
                     /gene="tenA"
                     /locus_tag="ACL297_000550"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HGR0070858.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            25048..25572
                     /gene="thiW"
                     /locus_tag="ACL297_000551"
     CDS             25048..25572
                     /gene="thiW"
                     /locus_tag="ACL297_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HGR0070859.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            25572..26375
                     /locus_tag="ACL297_000552"
     CDS             25572..26375
                     /locus_tag="ACL297_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGR0070860.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            26368..27000
                     /gene="thiE"
                     /locus_tag="ACL297_000553"
     CDS             26368..27000
                     /gene="thiE"
                     /locus_tag="ACL297_000553"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGR0070861.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(27210..28001)
                     /gene="thiD"
                     /locus_tag="ACL297_000554"
     CDS             complement(27210..28001)
                     /gene="thiD"
                     /locus_tag="ACL297_000554"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGR0070862.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(28085..28183)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            28367..28762
                     /locus_tag="ACL297_000555"
     CDS             28367..28762
                     /locus_tag="ACL297_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HGR0070863.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            28773..29144
                     /locus_tag="ACL297_000556"
     CDS             28773..29144
                     /locus_tag="ACL297_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HGR0070864.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            29154..31397
                     /locus_tag="ACL297_000557"
     CDS             29154..31397
                     /locus_tag="ACL297_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGR0070865.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            31604..33379
                     /gene="spxB"
                     /locus_tag="ACL297_000558"
     CDS             31604..33379
                     /gene="spxB"
                     /locus_tag="ACL297_000558"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HGR0070866.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            33490..33837
                     /locus_tag="ACL297_000559"
     CDS             33490..33837
                     /locus_tag="ACL297_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGR0070867.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(33954..34955)
                     /locus_tag="ACL297_000560"
                     /pseudo
     CDS             complement(33954..34955)
                     /locus_tag="ACL297_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            35251..35556
                     /locus_tag="ACL297_000561"
                     /pseudo
     CDS             35251..35556
                     /locus_tag="ACL297_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            35688..36632
                     /gene="manA"
                     /locus_tag="ACL297_000562"
     CDS             35688..36632
                     /gene="manA"
                     /locus_tag="ACL297_000562"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGR0070868.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(36654..38129)
                     /locus_tag="ACL297_000563"
     CDS             complement(36654..38129)
                     /locus_tag="ACL297_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HGR0070869.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            38212..38966
                     /locus_tag="ACL297_000564"
                     /pseudo
     CDS             38212..38966
                     /locus_tag="ACL297_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(38986..39441)
                     /locus_tag="ACL297_000565"
     CDS             complement(38986..39441)
                     /locus_tag="ACL297_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGR0070870.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(39461..40636)
                     /locus_tag="ACL297_000566"
     CDS             complement(39461..40636)
                     /locus_tag="ACL297_000566"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGR0070871.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFTGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(40695..41540)
                     /locus_tag="ACL297_000567"
     CDS             complement(40695..41540)
                     /locus_tag="ACL297_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGR0070872.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            41665..42222
                     /locus_tag="ACL297_000568"
     CDS             41665..42222
                     /locus_tag="ACL297_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGR0070873.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            42241..42708
                     /locus_tag="ACL297_000569"
     CDS             42241..42708
                     /locus_tag="ACL297_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGR0070874.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            42797..43426
                     /gene="upp"
                     /locus_tag="ACL297_000570"
     CDS             42797..43426
                     /gene="upp"
                     /locus_tag="ACL297_000570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGR0070875.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            43600..44190
                     /gene="clpP"
                     /locus_tag="ACL297_000571"
     CDS             43600..44190
                     /gene="clpP"
                     /locus_tag="ACL297_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HGR0070876.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            44269..44517
                     /locus_tag="ACL297_000572"
     CDS             44269..44517
                     /locus_tag="ACL297_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HGR0070877.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            44618..45778
                     /locus_tag="ACL297_000573"
     CDS             44618..45778
                     /locus_tag="ACL297_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0070878.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT        13859 a         8462 c         9909 g        13654 t
ORIGIN      
        1 tatgaagaaa ctattcatat tattatcaac tttttttctc agcttcttcc ttgcttggat
       61 tattgtctta cgtgcgccac aatatttata tgcaagctat gattccgttt ctttacttcg
      121 tgtcaaaaaa gatactcagg aaccgacgcg tgaggtattt gaacaggaat tggagaattt
      181 tgcaaactca gaacagagtt taatagctag aagaattgta gagccgagta aggatgggac
      241 gactcacttt acttatgcaa cttatggtca gggaacttta ccaaaagaat tccaagaagc
      301 tagccaagaa agtcgtgaac gtagtgatcc gctaaatagt tatctccttt tgtcaggctc
      361 cttgacgaaa gaaaagcttg ccgataaatt aggagatttg ggttataaag caagtgctga
      421 ccgaaagata ccgccctatt ttcttgcttt tcgaatatta ctaaatcccc ttattttaat
      481 tagtttagca atatttggct tatctttctt tgctttagtg attatcactc ggattaagga
      541 aatgagagca gcaggtataa aactcttttc tggtcagact ctcttatcca tcatggggca
      601 ttctttatct actgatatca aatagctcct tctatcagcc ctcctttcct tcctaggtgg
      661 gggagtcgtt ctttttagtc aaggtttgtt ttatcctatc ttgttagcca cctatggttt
      721 tgggattagt ttctatctgt tgtttttatt ggcgatttca attttactaa tgcttcttta
      781 tctaatgagt ttgagttaca aagcattagt tcccgttatt aaggggagat taccccttaa
      841 acgcctgatg attttaaccc tattgtgtca gttggttgct gtctttacag taggatacgc
      901 tgttaagaca ggtttgacgt cttaccaacg attgaaagaa cttgaaattt caaaacaagc
      961 atggcaggat agagcagact attatcaaat ttcttttggc ttaggtgata gaggaaaaga
     1021 tacagaaaat cagagcaagt ggtatgcctt tgccaaggaa gcaatcgaag aggaacaagc
     1081 tctttatgta aaggataatc tgctccattt tgccaatcca caaggaaaaa atgaacaggg
     1141 agagacactg gatacctata gtccagatgc taatacgctc tatgttagtc ccagttattt
     1201 ggacaaggaa aaggtcgtgg tagatgctga gaccaaacag aagttagccc atctccaaaa
     1261 aggtgagttt atcctcttgc tcccagaaca tttgcgctct cgagaagcag aacttaagaa
     1321 agtttttgaa gaaagattga gttattatgg aaaatctggt gaggaggcaa gtgctccttt
     1381 ggattatgag atgaaagcgc acgttagtta tctttcaatg ggagaaaagc ggtttgttta
     1441 taataacggt gagaatcccg tatctactca gtatttgact gatccgattt tagtggtatt
     1501 cacgccgact tctacaggtg atagttttat atccttatct agttggtcta tcaatgctgg
     1561 aaaacaactc tttatcaaag gatatgagag tgggctagaa ctcttgaaga aagctggaat
     1621 ttatgagcaa gtatcctatc ttaaagaagg aagaagtgtt tatctaactc gttataatga
     1681 agttcaaact gaaacagcaa ctttaatctt aggagctatt gtggggatag ctagttcctt
     1741 attactattt tattctgtca atcttctata tttcgagcaa ttccgccgag atatcttgat
     1801 taaacgaatt tcaggtttac gattttttga aacacatgct cagtatatgg ttagccaatt
     1861 tgccagtttt gtatttggtg ctagtctctt tattttaagc agtcgagact tggtgattgg
     1921 cttgctcact ttattggtct ttctagctag tgcagttttg acgctttacc gtcaagcgca
     1981 gaaagaatct cgtgtttcta tgacaattat gaaaggaaaa taggatgatt gaactaaaga
     2041 atatatctaa aaaatttgga agccgtcagc tattttcaga tacgaatctt cattttgaag
     2101 gtgggaaaat ttatgcctta atcggtacaa gtggctgtgg taagacaaca ctcttgaata
     2161 tgattggacg attggagcca tatgacaaag ggcaaatcat ctatgatggc acttctctta
     2221 aggacatcaa gccttctgtt ttctttagag attacttagg atacttattt caagattttg
     2281 gcttaattga aagccaaacc gtcaaagaga atctcaatct gggtttagtt ggtaaaaagt
     2341 tgaaggaaaa agagaaaatc tctttgatga aacaagctct aaactgtgta aacctctctt
     2401 atttggattt gaagcaacct atatttgagt tatcaggagg agaagcacaa cgtgttgcac
     2461 tagcgaagat aattttaaag gatccgcctt tgattcttgc agatgaacca accgcttcct
     2521 tagaccccaa aaattctgag gaattacttt ccatcctaga atctttaaaa aatccgaatc
     2581 ggaccattat tattgcgacc cacaatcctc tgatttggga gcaagtggat caggtcattc
     2641 gagttaccga tttatcacat agatgatatg gtaagattca gttagaagaa agagtcacaa
     2701 acacactttg tggctttttt atttccataa aaatggtaaa atagtaggag tagaaatgga
     2761 gttcgagaca tgaaagtaat agatcaattt aaaaataaga aagttcttgt tttaggtttg
     2821 gccaagtctg gtgaatctgc ggctcgtttg ttggacaagt taggtgccat tgtgacagta
     2881 aatgatggga aaccattcga ggacaatcca gctgcccaat gtttgctgga agaagggatc
     2941 aaggtcatta caggtggcca tcctttggaa ctcttggatg aagagtttgc cctcatggta
     3001 aaaaatccag gtatccccta cagcaatccc atgattgaaa aggctttggc caagggaatt
     3061 ccagtcttga ctgaggtgga attggcttat ttgatttcag aagcaccgat tattggtatc
     3121 acaggatcga acggtaagac aaccacaacg actatgattg gggaagtttt gactgctgct
     3181 gggcaacatg gtcttttatc agggaatatc ggctatcctg ccagtcaggt tgctcaaata
     3241 gcatcagata aggacacgct tgttatggaa ctttcttctt tccaactcat gggtgttcaa
     3301 gaattccatc cagagattgc ggttattacc aacctcatgc caactcatat cgattatcat
     3361 ggattatttg aggaatatgt agcagccaag tggaatatcc agaacaagat gacagcagct
     3421 gatttccttg tcttgaactt taatcaagac ttggtaaaag acttggcttc caagacagaa
     3481 gccactgttg taccattttc aacacttgaa aaggttgatg gagcttatct ggaagatggt
     3541 caactctact tccgtggtga agtagtcatg gcagcgaatg aaatcggtgt tccaggtagc
     3601 cacaatgtgg aaaatgccct tgcgactatt gctgtagcca agcttcgtga tgtggacaat
     3661 caaaccatca aggaaactct ttcagccttc ggtggtgtca aacaccgtct ccagtttgtg
     3721 gatgacatca agggtgttaa attctataac gacagtaaat caactaatat cttggctact
     3781 caaaaagcct tatcaggatt tgacaacagc aaggtcgtct tgattgcagg tggtttggac
     3841 cgtggcaatg agtttgacga attggtgcca gacattactg gactcaagaa gatggtcatc
     3901 ctgggtcaat ctgcagaacg tgtcaaacgg gcagcagaca aggctggtgt cgcttatgtg
     3961 gaggcgacag atattgcaga tgcgacccgc aaggcctatg agcttgcgac tcaaggagat
     4021 gtggttcttc ttagtcctgc caatgccagc tgggatatgt atgctaactt tgaagtacgt
     4081 ggcgacctct ttatcgacac agtagcggag ttaaaagaat aaaatatgaa aaaaattgtc
     4141 tttacaggtg gggggacggt tggacacgtt accctcaatc ttttgttaat gcccaagttc
     4201 atcgaagatg gttgggaagt ccactatatc ggggacaagc gtggtatcga acaccaagaa
     4261 atccttaagt caggtttgga tgtcactttc cactccattg cgactgggaa attgcgtcgc
     4321 tatttctctt ggcaaaatat gctggacgtc ttcaaagttg gctggggaat cgtccaatcg
     4381 ctctttatca tgttgcgact tcgtccacag accctttttt caaagggggg ctttgtctca
     4441 gtaccgcctg ttatcgcagc gcgtgtgtca ggagtgcctg tctttattca cgaatctgac
     4501 ctgtctatgg gcttggccaa taaaatcgcc tataaatttg cgactaagat gtattcaacc
     4561 tttgagcaag cttcgagttt gtctaaggtt gagcatgtgg gagcagtgac caaggtttca
     4621 gatcaaaaaa atccagaacc agatgaattg gtggatattc aaacccactt taatcataaa
     4681 ttgccgactg tattgtttgt tggcggttct gcaggtgctc gtgtctttaa ccaattggtg
     4741 acagaccata agaaagaact aacagagcgc tacaatatta tcaatctaac tggagattct
     4801 agcctgaatg agttgagcca aaatcttttt cgtgttgact atgtgaccga tctctatcaa
     4861 cccttgttgg aattggctga tattgttgtg acacgaggtg gtgccaatac gatttttgag
     4921 ctcttggcga tagcaaaatt gcatgtcatt gtgccgcttg gtcgtgaagc tagtcgtggt
     4981 gaccagattg aaaatgcagc ttactttgta aaaaaaggct atgcagaaga ccttcaagaa
     5041 agcgatttga ccttggatag tttggaagag aagctttctc acttactaag tcacaaggaa
     5101 gattaccaag ctaagatgaa agcttctaag gaattgaaat ctctagcaga tttttatcaa
     5161 ttgttgaaaa aagatttatc ataaggaaag taaatgtcaa aagataagaa aaatgaggac
     5221 aaagaaatcc tcgaagaatt gaaagagtta tcagaatggc agaaacgaaa ccaagaatat
     5281 ctaaaaaaga aggctgaaga agaagcggct ctagctgagg agaaggaaaa ggaaagacaa
     5341 gctcggatgg gagaagaatc tgagaagtca gaggacaaac aggaccagga gagtgaaaca
     5401 gaccaggaag attcagaatc agctaaggaa gagtctgaag aaaaagtagc atcctcagag
     5461 gatgacaaag agaaagaaga gaaagaagaa ccagagtcta aagagaagga ggaacaggat
     5521 aaaaagcttg ctaaaaaggc tacaaaggaa aaaccagcca aagcaaagat tcctggtatc
     5581 catatcttgc gagccttcac gattttattt ccaagtctgc ttttattgat tgtctctgcc
     5641 tacttgctca gtccttatgc gaccatgaaa gatattcgtg ttgagggaac ggtgcaaact
     5701 acagctgatg atattcgaca ggcttcaggc attcaggatt cggattatac gattaacctt
     5761 ctgctagaca aggcaaaata tgaaaagcag attaagtcta actattgggt tgaatcagct
     5821 caacttgtct atcaatttcc aactaagttc actattaagg tcaaggaata tgatattgtg
     5881 gcctactata tttctggtga aaatcattat cctattcttt ccagtggtca gcttgagact
     5941 agttctgtga gcctgaacag tttaccagaa acttatttat cagttctctt taatgatagt
     6001 gaacaaatca aggtttttgt ctcagaactt gctcaaatta gcccagaact caaggcggct
     6061 atccaaaagg tggaattagc cccaagcaag gtgacatccg atttaattcg attgactatg
     6121 aatgatttgg acgaagtctt ggttccgctt tctgaaatga gcaagaaact gccttattat
     6181 agtaagatta agccacaatt gtcagaaccg agtgtgattg acatggaagc tggaatttac
     6241 agttacactg tggcggataa attaattatg gaagctgagg aaaaagccaa acaagaggct
     6301 aaggaagctg agaaaaaaca agaagaagaa cagaaaaaac aagaggaaga gagcaatcga
     6361 aaccaaacga cccagcgctc atcgcgtcgc taggtttacc ttttctctta tagttcttta
     6421 gtgaccatgt ttttacttga caagtgctag tagaaataat agaatagtaa aaaaccttta
     6481 aagcagtcca gagaggcagc taaggttaga cggtgaaagg gtggagacta cccatttttc
     6541 gtggaacctt gctgttggca ggttcctttt ttcgtggctt ctgttggcca gactctctca
     6601 ctagtaaagg taaaaggaga aacctatgcg agaacatcgt ccagtcattg ctcttgattt
     6661 tcctagtttt gaggcggtca aggaattttt agctcttttc ccagcagaag aaagccttta
     6721 tctcaaggta gggatggagc tttattacgc agcggggcct gagattgtgt cctacttaaa
     6781 aggtttgggt catagtgtct ttttggatct caaacttcat gacattccta atacagtcaa
     6841 gtcagccatg aaggtcttgt ctcagcttgg tgtcgatatg actaatgtcc atgcggctgg
     6901 tggtgtagag atgatgaagg cggcgcgtga aggccttggg agtcaagcta aattgatcgc
     6961 tgtaactcag ctcacatcaa cgtcagaagc tcagatgcag gagtttcaaa atatccaaac
     7021 cagtctgcaa gagtctgtga ttcactatgc caagaagaca gctgaagctg gcttggatgg
     7081 tgttgtttgc tcggctcagg aagtacaagt catcaagcag gctaccaatc cagattttat
     7141 ctgtctgaca ccagggattc gtccagctgg tgctgcagtt ggagatcaaa aacgagtcat
     7201 gacacctgct gatgcctatc aaatcggcag tgactatatc gtagtgggac gtcccattac
     7261 ccaagctgag gatcctgttg cagcttatca taccatcaag gatgaatgga cacaggactg
     7321 gaattaaaga actagattag aaaaataaaa ggagaatacc atgacacttg ctaaagatat
     7381 cgctagccac ctcttgaaaa tccaagccgt ttacctcaaa ccagaggaac ccttcacttg
     7441 ggcatctggt atcaagtcac cgatttacac tgataatcgt gtgacactag cctatccaga
     7501 aactcgtacc ctaattgaaa atggttttgt ggaagctatc aaagaagcct ttcctgaagt
     7561 agaagtgatt gcaggaactg caacagcagg gattccacac ggagccatta ttgctgataa
     7621 gatggacttg ccttttgcct acatccgtag caaaccaaaa gaccacggag ctggtaatca
     7681 aatcgaaggt cgcgtagctc aaggtcaaaa aatggtagtg gttgaagacc ttatttcaac
     7741 gggtggttca gttcttgaag ctgtagcagc agccaagcga gaaggagcag atgtgcttgg
     7801 agttgtagcg attttcagct accaattgcc aaaagcagat aagaactttg cagatgctgg
     7861 tgttaaactt gtgacgcttt caaactatag cgagcttatc catctagccc aagaagaagg
     7921 ttacatcacg ccagagggcc ttgatcttct aaaacgcttt aaagaagacc aagaaaattg
     7981 gcaagaaggt taggtcagta agataaagag agatgaggct accgagcctc ttttatcatt
     8041 ttatttaaaa tatgacaata ttttaaataa aatgattgac aacgctttct tttgttgata
     8101 taatgaaaat gtaatatgta tcaaattgat caatcaacta tcttgtttat cacttcttag
     8161 gaggacgatg tcatgaaaag aagaaaaata tattcctttt ttgcgtagtt acaattgcat
     8221 ctagtttgtt tctccttaca gttttagcta gacagattgg agattatgat ttaacgttgc
     8281 cgcgttgggg ttcggataca actagtgagc ttgagaaaga aaacgcctct gctggaatta
     8341 ataataatga cagcactggt ggcggtaaaa ggttaaatac ctctattcgt agcgcctata
     8401 gtgggtcaga tattaccccg gtatattcat tggggtctgg ctctaggatt gtcatgtact
     8461 ataatggagg tggtgacaat tatattggtt ctggtactag attagctatg gcgccacaat
     8521 ttggaaatca tgtaagaatt catacttcag gttcttggaa tccagattct tattaactta
     8581 cttgtcagag taagccttag agatggttga ttgtgggtgt agcatgaaaa aagaatgcta
     8641 caccctattt ttattataag gaggagtaag gatggaattt ttcatttgta atcttgtacg
     8701 agtcgttcaa tcacctcgat tttatatgtc tttatttttg acccttcttt gcatgagttt
     8761 aggaaatttc cttgctttca atggtattta taaaattgaa ggtttatcga ttttttttgc
     8821 cgcttcttct attcgaggat tttcaccgat tagcctagta gctgcactta tctgtacact
     8881 gccctattct agtcagataa tagaggatgc tgagagtcat tttctaacag cacaattgtg
     8941 tcgaatttct aaaaagaagt atctggctat tgtgggtagt actgtaatta tttcttcttt
     9001 tctagtcttt tttctcccct atttattatt attaggaatt aatcttttag tgactcctta
     9061 tcaggaaatt tatattggag attatagtgg tgccttaaaa gaattatttg attccaatca
     9121 gtttctctat agtcttgtaa cgactctctg gtatggagtt tggggcgctg tgttctctat
     9181 ttttggacta gctagtgctt tgctagtgaa gaaaaaaata ggagctattt tcatcccagt
     9241 tgcctatatg atggttggtg gtattttttg ggctatttta gggctatctt acttagaacc
     9301 tgtgacaaca ttagctttgg gatatcagaa agatatcagt ctttccttag ttagtgggca
     9361 tcttgctttt attttatttg ttagttgttt ggttgtttat ggtacatttt ttctacattc
     9421 agaggactac gtataatgaa acaatttgtt caattttaca aaaaagattt tttatcagta
     9481 ttggtttatt ttatattact gctatcctgt gttttatcta gtacagtata tttattgcgc
     9541 tgtcgccaat attcaatcca tccaaatgta ttagaatgga tcttagtttt acttcaagat
     9601 atgacgactg gagtatattg ctttccgttc acatatatat tgttcttttt ttatttgatg
     9661 aataactatt ttaataggtt ggagtgtcgc attcgtctga aatcaattaa gcactttacc
     9721 agttttagtt tcaaattagc agctcttagt acggggattt ggacggcgac tttattttta
     9781 ttgatttttc taattgcatt tagtaatggt tttagcttct ctttggagat aaaggaggtt
     9841 gattttttaa gagaatttta tggtataagt attgcaaaca atgctagttt ctttatagga
     9901 ttttttttct cttatatagc atactatttc tttttatcct tacttactat tagcagtttt
     9961 tcttggttta aaaaatcaaa catgagctta gtatttctgt ttactttttt atttgtagaa
    10021 tccttattct ggatttatca gttggacaat gggataattg gattattgcc aatttttcag
    10081 tatatggtaa attccaatcc gtatgcattg atttattggc ttacattact atctatcata
    10141 attccattga ctgtattttc tgttcataga aactggagga gagtgtaaaa gttggaaatg
    10201 ggaaagttaa gtagtcacat gtggaggttg aatcagataa tctataccaa gtacttttgg
    10261 ggttatgttc ttttttggat attgatttgt ttaggattat ggtattggtt agaaggaaat
    10321 gatagacttg ttatagaaat tttaaaaggg cctaatctga gtcaaaactc ttttttagtc
    10381 ttatctatat ggttgcttca ttggtttatt attcatacat tttttctagc agttgtatat
    10441 cgtagaagag catccgattt ctttatggaa gtgattcgat tttcttctat taagctctgg
    10501 attaggtatc agatttggac ctgttttctt tatggactca ttttaatcat ggtaaaagtt
    10561 ctagtgattc aatttatgtt acagttacca aactgggata taggagtttt gtttatagtt
    10621 gattctttga atgcttgtgt gttagtcttg ttttgcttta tgttatacgc actaggagcg
    10681 aatgtacaaa tgaactttgc ttgcgttagt ttctttttac tcatgattgt gtttggtggt
    10741 ctatttgtag gaaatcgaac taattatcta ttttatattt tgaatcgagg gaatggagat
    10801 ataggacgag atttatttct tcagttgcta tttttagtct ttctttttaa aagtattttc
    10861 tatttcactc gtcaaaagag gaggtttata gaatgaatga aataatcata ttaaaaaata
    10921 ttgagttgaa attaaaaaaa acatgtgttt ttcaaaacct taattttagt tgtaaacagg
    10981 gggaaattat aggaattact ggtgcgaatg gctcagggaa aagtgtattg tttaaattaa
    11041 tagctggttt atatagtccg tcttatggag aagtgttaat caatggggaa aatattgttc
    11101 ctgagagaaa aattccagct aatttgggag ctttgattga agaacctggt tttataaatt
    11161 attatagtgg ctttaagaat ttacaatatt tggcaagcat acgaggagta gttggtaatc
    11221 aggaaatcaa tgatacactg aaaatagttg gtctatatga gcaaaaagac cagaaagtta
    11281 aaacttattc gctaggtatg aggaaaaagc tagggattgc tcaagcaatt atggagaatc
    11341 cctctattct tttactagat gaacctatga atgccttgga taaatcaagt gtagaaaata
    11401 tgagaacatt gtttagaaag ctctctagtg aaaaagggac aacaattttg attgctagtc
    11461 atagtgaaga ggatattcgt atcttatgtg ataaagtata tgcaatagaa gataaagtat
    11521 gtacactgtg ttcagattga acatcttaga tagactcagg atgttatagg taaaatctta
    11581 aaaagggatt ggagcttaaa atccagtccc ttttaagagt taatggccac cttcaacaac
    11641 caggtcatct gctttagcag cgtcaccgta ggttgggtga agtgtttttt cataggcctt
    11701 gtggaagaag ttttccttgc ctaatttttc aatatcttta ttgatgaagt ctagcaattc
    11761 ttggttgcct ttttgaactg ctgccgcaat ggtatcggga tcaccgaggg aagtaattcc
    11821 tacttcaaat cctttatttt caagcgccca agctagaact tccgtattgt cagttgaaaa
    11881 ggcatctcca cgtccgtcaa gaagagcttg gtaagagtca ctgtattggt cgtatttttg
    11941 gagtttgatt tctggatgat tcttttcaaa ataagtctca gcagtcgttc cttttgtgac
    12001 aattaaggtt ttaccttcaa gttgtttgac gtctgtaatg agaccagtct taggtgatac
    12061 gacacccaga gaaactttca tatatggaag ggcaaaatca acttgtttct tacgttcgtc
    12121 agttactgta aagttagcaa gagtaatatc taccttgttt gaaatcaagt attccgcacg
    12181 gttggcagca tcgactgaaa tgtatttaac cttgacacca aggtctttag ctagttggtt
    12241 ccctagttca atatcgtagc cttggtaaga accatcattg tcaacgtagc caaacggttt
    12301 tttatctcca aacacggcga ttcgcagttc accgcctttt tttgatttca tcgatagtgc
    12361 gagctttggc agttgttttt ccagaggatg aaccagcgtt tccacctgag ctacaagcag
    12421 tgataaagat aagggcaaag gcaagtgcta aaacagttaa gagtggtttg aatagtttca
    12481 taagaatctc ctttatagat atgagccaaa ttggctaaag tcaaagacgt ttaaaaattc
    12541 ctgggctcgt ttggtttgcg gattggtaaa gaaggcttga gctgttcctt cttcagcgat
    12601 tttcccttgg tcgaggaaga taatccggtc agcaatggct tgggcaaact gcatttcgtg
    12661 ggttactaaa atcatggtac ggccttcttg ggccaaatca ttgataagtt ccagcacctc
    12721 acgcaccatt tctggatcca gcgaagcagt cacctcgtca aaaaggatga tttctggatg
    12781 cattaggagg gcacggacaa ttgcaacacg ttgcttctgt ccaccagata attgacgggc
    12841 aaagctatgt tgtttatcca gcaaaccgac acgatctagt aattgcaaag cttcttccgt
    12901 tacttctttc ttgtcccttc cttgagtttt gatagggcct aggatgaggt tttgtaagac
    12961 atccagatgg ggaaagagtt cataactttg aaagaccatg ccaatctttt ggcgaactag
    13021 gtgaaaattt tttttatttt caacgataga ctgaccatcc agaaaaatat ctccaccttg
    13081 aatactttct aagccgttga ggcaacgaag gagggtactt ttcccacaac cagatggccc
    13141 taggatgaca acaacttccc cttttttgat ttctagagaa agtccttgga ggatgggatt
    13201 gtctccgaag gattttttta gttccttgat ttctaagata gtttcagaca tttagtttct
    13261 ccaatgtttt tctaagtgag tggatagttt ggaaataggg tagcaaactg cgaaatataa
    13321 gactagaatg gttccataaa tccaaaatga agcagttggg atggtcaggc gattgctatc
    13381 gatgatttgt tgtccaactt tggtcacttc cacaacccca atcaaaacaa ctaatgaagt
    13441 ggttttaatc atccgagtga caagattgat agcctgcggt agcagtcttc tcaagacttg
    13501 tgggatgatg atgtggtagt aaagttgaac attagtcaag ccgagtgcct gtccactttc
    13561 aaactgatgt ttagggagag aagtgatagc tccacgtacc aagtctccca tttcagctgt
    13621 tccccagagg gtaaaaacga taatagctga agtctcacct gagatattga tattaaagtt
    13681 tcgagccaag ccaaagtaaa cgatgaagag tagcaccagc tggggcatga tacggataaa
    13741 ttccagatac aatcgtgtta aaaatcgtat gattctagaa tgggaggtca tgatgattcc
    13801 catgactgtt ccgaacatca tggataagag gacagacagg atggatatcc caatcgtaac
    13861 gcccaatccc tgtaagattc tcaggagatt atttccttga aagagtactt ggattcccga
    13921 atcctgcatg gcggagcctc ctttctatcc agctaaagac cagtgagatg ggtagcagca
    13981 tgatcagata agcaactacc aacatagcta gcgcaatgtc tgtctcatag tagagaccaa
    14041 tcaaatcctt ggcgacgtac atgaggtcgg ccaaagccac tgctgagaaa acagaggttt
    14101 ccttgataag gaaaatgaca ttggcactaa aggaggggag tgccaccgct gttgcttgcg
    14161 gaagaaccac atagtaaaag acctgtagag gtgtcagacc aatagcgagg ccaatctcct
    14221 gctgggtttg actgatggct tccagcccac ttcggaaaga ttctgccata taggagtctc
    14281 ctaaaaagac aagcccaagc gttgcacaga cttctgaaga taggacaatc ccgattcggg
    14341 gaagaccgaa gtagagaaag aagagttgaa tcaaaagggg cgtattacgt gacaattcaa
    14401 tgtaggctgt cgctacttgc gacaaaacaa ggattcgata atgtcggatg atactaacga
    14461 ttaaaccgag cagaaaggat cccaaaattc cccaaactgc aatatgcaag gtcagaaaga
    14521 atgccttttg atatagtggt agatattgtt caacaatgga tcaatccaaa aatagaacct
    14581 cccatctaga aataatacag ttattgtagc acttaaaatc ttctttggat aatatctatt
    14641 ttttattgcc gttataagga tttttatcat agacataaaa tttctgaaat ttccaaacaa
    14701 aatattttaa aagttttgaa aaagagttaa gatatttttg taatacacaa agtaaacgct
    14761 tacttattaa ggaggacatt ttatgtcata caaaacaagc aatgcagaag gtcatgtaga
    14821 tttcatcaat acctatgatt tggagccaat ggcgcaacaa gttattccta aagcagcatt
    14881 tggctatatc gctagtgggg cgggagatac tttcacttct ttccagtgat tttagcgtca
    14941 ggttcttttt agtttttaaa gattatccgt gaatttcttg cttatttatg ataaaatggg
    15001 agtatcgcaa aaaatgactc atcgtattca attttgagta aaactaggag gatcccatgt
    15061 ctacagaaca tatggaagaa ctaaatgacc agcagatcgt tcgccgtgaa aaaatggctg
    15121 cgctccgcga acaaggaatc gatcctttcg gaaaacgttt tgaacgtact gtaaattcac
    15181 aagaattaaa agataaatat accaacctcg ataaagaaca attacacgat aaaaacgaaa
    15241 cagctactat cgcaggacgc ttggtaacca aacgtggtaa aggaaaagtt ggttttgccc
    15301 accttcaaga ccgcgaaggc cagattcaga tctacgttcg taaggatgct gtcggtgaag
    15361 aaaactacga aatcttcaaa aaagcagacc ttggtgactt ccttggtgtc gaaggtgaag
    15421 tgatgcgtac ggatatggga gaactctcta tcaaggcaac ccacatcaca cacttgtcta
    15481 aggctcttcg tcctcttcct gagaaattcc atggtttgac agacgttgaa acaatttacc
    15541 gtaaacgtta ccttgacttg atttctaatc gtgaaagctt tgaacgcttt gtcactcgtt
    15601 caaaaatcat ctctgaaatc cgtcgttacc ttgaccaaaa aggattcctt gaagtggaaa
    15661 cacctgttct tcataatgaa gccggtggtg ctgctgcccg tccatttatc acccaccaca
    15721 atgcccaaaa cattgacatg gtgcttcgta tcgcgactga gcttcactta aaacgcctta
    15781 tcgtgggtgg tatggaacgt gtctatgaaa ttggccgtat cttccgtaac gaaggaatgg
    15841 acgctactca taaccctgag ttcacttcta tcgaagttta ccaagcttat gcagacttcc
    15901 aagacatcat ggacttgact gaaggcatta tccaacacgc tgctaaatca gtcaaaggtg
    15961 atggcccagt caactaccaa ggtactgaaa tcaagattaa cgaaccattt aagcgtgttc
    16021 atatggtgga tgctatcaga gaaattactg gtgtcgattt ctggcaagac atgactttgg
    16081 aagaagctaa agctatcgct gctgagaaga aagttccagt tgagaaacac tacactgagg
    16141 ttggtcacat catcaatgcc ttctttgaag agtttgttga agaaacttta atccaaccaa
    16201 cctttgtcta tggacatcca gtagctgtat ctccactcgc taagaaaaat cctgaagacc
    16261 aacgctttac tgaccgtttc gagctcttta tcatgactaa ggagtacggt aatgccttta
    16321 ctgagttgaa cgacccaatc gaccaactta gccgttttga agcccaagct aaagccaaag
    16381 aacttggtga tgatgaagcg acaggaatcg actatgacta cattgaagct cttgaatacg
    16441 gtatgccacc aacaggtggt ttgggaatcg gtatcgaccg tctctgcatg ctcctcactg
    16501 atacaacaac tatccgtgat gtattgctct tcccaacaat gaaataaatt cttatcctct
    16561 ggctcttacc aggggatttt ttgattcaaa aagagactga atttaaggag aaaatgaagt
    16621 gtagtatatt gaagttgaaa tagtacactt tgatttctaa gacattgtta gaaattggtt
    16681 taaattccct aagcaatttg tgcatgtttt atttcatttt acgatagtac gctgaaactg
    16741 ttcaaaaagt actagaaatt gacttggatt ccccaattga tttgttcaga ttcactataa
    16801 ataaaaaatt aataagtggg ataggaagtt agcgtcaact aggatagtat cttgcttaaa
    16861 cagtatatat gggactgata taagtccata ggtcctatta gaggatgttc tggtgtctta
    16921 ttcacttgtt tttttatagt attagtagat agaatccgca aataaaaaag tagttcaaat
    16981 gcctggcagc aaaaaatctc aacgattcac ttaatataga ctaaggttta gagtttaagg
    17041 aaagtagggc tagtatattg tatgaaaaaa taagatggtt tttaagcaaa actcttgaaa
    17101 atgattgaaa aaagagttat tatagttgtg tagtaaaaag caaacgctta cttaatcaag
    17161 gagggcattt tatgtcatac aaaacaagca atgcagaagg tcatgtagac ttcatcaata
    17221 cctatgattt ggagccaatg gcgcaacaag ttattcctaa agcagcattt ggctatatcg
    17281 ctagtggggc ggaagatact ttcactttaa gagagaatat ccgcgccttt aaccacaaac
    17341 tcatcgttcc tcatacactt tgcgatgttg aaaatccaag tacagagatt gaatttgcag
    17401 gtgaaaaact atcttcacca atcattatgg cgcctgttgc ggcccataaa ttagcgaatg
    17461 aacaggggga agtggcgact gcgcgtggtg tgcatgagtt tggttctctt tatacaacca
    17521 gttcttactc tactgttgac cttccagaga tttctgaagc ccttcaaggg acaccccatt
    17581 ggttccaatt ttactttagt aaggatgacg gtatcaaccg ccacatcatg gatcgtgtga
    17641 aggctgaagg ttataaagcg attgtcttga cggcagatgc tactgtaggg gggaatcgtg
    17701 aagtggataa gcgtaatggt tttgtcttcc cagttggcat gccgattgtt gaagaatacc
    17761 tgccagaagg tgctggtaaa tcaatggact ttgtttacaa atcagctaaa caacgcttgt
    17821 ctccacgtga tgtagaattt atcgctgaat actcaggact tcctgtgtat gtcaagggac
    17881 cacaatgccg tgaggacgtt gaacgttcgc ttgctgcagg agcttctggt atctgggtaa
    17941 ccaaccacgg tggtcgtcaa atcgacggtg gaccagctgc ctttgactcg cttcaagaag
    18001 tggcagaagc agttgataaa cgtgtgccaa ttgtctttga ctctggtgtt cgtcgtggtc
    18061 aacatgtctt taaagccttg gcttcaggag cagacttggt agctattggt cgcccggtta
    18121 tctatggctt ggctctcggc ggtagtgttg gtgtgcgtca agtctttgaa cacttgaatg
    18181 cggaattgaa gacagttatg cagttgtctg gaactcagac tattgaagat gttaaacact
    18241 tcaaactccg tcacaaccca tacaacccaa ctttcccagt tgaccctcgt gacttaaaat
    18301 tgtattgata aaagagattg cctccactga atgtggaggt ttttcttcgt gtattggaag
    18361 aataaatcgc tatcgaaaac catagtaaaa tggaataaaa acaggacaaa tcaatcagga
    18421 cagttaaatc gatttttaac aatgttttag aagtagatgt gtactattct agtttcaatc
    18481 tactatatat aaagatattt ttcggtcaaa tatcttgcta gggctttcat tttttgctat
    18541 actggttcag taattaaata atagagacat ttggggtgct ttaagctgag atgataccca
    18601 ttgaacctga tacagttaag actggcgaag ggaaatgtga aacgtttttt cgtatgacga
    18661 aatgaacgat ctaatcttgt aagtcaactc taattcttga ttgtaattgg agttttttgt
    18721 ttatataaac tggataggct tgtttagtta gctctctgca aatgctctcg atgtgcttta
    18781 aaaggatcaa gtggaattac gattttgctt gatatttctt ggtgttttat taattactaa
    18841 caagtaaaga gaggagaagt aaatatggaa acacaagact atgcatttca gccaggtttg
    18901 actgttggag aattattaaa aagcagtcag aaggattggc aggctgcaat caatcatcgt
    18961 tttgttaagg aactttttgc ggggacaatt gagaataagg tcttaaagga ctatctgatt
    19021 caagattatc acttctttga tgccttctta tccatgctgg gtgcctgcgt agctcacgca
    19081 gataaacttg aatccaagct tcgttttgcc aagcaactag gctttcttga agcagatgaa
    19141 gatggttatt tccaaaaggc ttttaaagag ttaaaagtag cagagaatga ctatctagaa
    19201 gtgactttgc accctgtaac aaaagccttt caggatctaa tgtattcggc agtggcttca
    19261 tcagactatg cccatctttt gattatgctg gtcattgcag aaggtctcta tttagactgg
    19321 ggttctaaag atttggcttt acctgaagtc tatattcatt cggaatggat caatctccac
    19381 agaggtcctt tctttgcaga gtgggttcaa tttctggttg acgaactcaa tcgtgtcggt
    19441 aaaaatcgag aagatttgac agaacttcag caacgctgga atcaagcagt cgctttagaa
    19501 ttagcttttt ttgatattgg ttacgatgtc tagagaaggc tcatgatatt tacaaatttg
    19561 atgaggtaag actcttcgaa aatctcttca aaccacgtca gttctatcca caacctcaaa
    19621 acagtgtttt gagcaacctg cggttagcct cctagtttgc tctttgattt tcattgagta
    19681 taaatcattc aatgtaaatg aaaaaaattc tcaactaaag ataaatcgaa attagtagat
    19741 cgtataaagt gaaaaggaag gatttcaata tgacaagttt aaaattatta aaagaaaaag
    19801 cacctttggt catttgcatc accaatgatg tagtaaaaaa tttcacagca aatggattag
    19861 tagcactggg cgcttcacca gccatgagtg agtttccaga agatttagag gacttattaa
    19921 agtatgctgg tggtttatta ataaacatag gaacattgac agatgaaaat tggaaattat
    19981 accaagctgc tctgaaaatt gcagagaaat ataatgtccc agcagtttta gatcctgtag
    20041 cctgtggagc aggagaatat agaaaaaaag tagcagatga tttaatcaac aattacaaac
    20101 tagctgcgat tagggggaat gctggagaga ttgcctcttt agtaggaata gatgtggcat
    20161 ctaaaggagt agatagtgcg ggagtagata atattgacga gattgctcta gcagcaaatg
    20221 agaagttcaa tattccaata gtagtaacag gtgaagtgga tgctattgcg gtaaatggag
    20281 aagtggtaac gattcataat ggtagtgcca tgatgccaaa agttattgga actggatgtt
    20341 tattaggagc tgtagtagca agttttatcg gactggaaaa aggtcaagaa ttaaaatcat
    20401 tagaaacagc aatgttagtt tacaatatcg ctggagaaat ggcagaaaaa cgtccaaatg
    20461 gacatcttcc tgggacattt aaagttgaat ttataaatgc cttatacgag attacagatg
    20521 aagatgtaaa ggaattcaaa agagtgaagt aagatgtttc ataaagaatt actaaaacta
    20581 tattttattt gtggaacgac tacttgccaa ggaaaaaatc tatatacagt cgttgaggaa
    20641 gcattaaaag gtggtataac cttatttcaa tttcgagaaa aaggtgaggg tgctttagaa
    20701 ggcttagaaa aacttgagct agctatccaa attaaagaac tttgtaaaaa atacaatgtt
    20761 ccatttatcg ttaatgacga tatagattta gcaatggaaa ttgacgccga tggcgtacat
    20821 gtaggtcaag atgatattgg tgttgatgaa attagaaaat tgatgccaga taaaataatt
    20881 ggtctttcta taagaaacga agaagaattt caacaatcaa aagttgaata tgtagattat
    20941 gtaggtgttg ggcctgtatt tgatacccag tcaaaagatg atgctggtgg tgctataggt
    21001 tatgaaggtc ttgaattgat gagaaaacta ttgccacaaa tgcctttagt tgcaattggt
    21061 gggatacaga cgaaacatat taaagacatt ataaagacca atatggatgg cgtttcaatc
    21121 atttcggcaa tatcgtatgc aaaaaatata gaaaaaactg ttcgggaaat gagtgaacaa
    21181 tagatatcta tccttggatt ctaagtaagt aggattttcc ttttttaaaa agctatgtcc
    21241 agattcagct tgaatggcta attcatcaaa caggtcattt gttttttgca aaagaaagat
    21301 ctgctgtctt tgtgagtgca aggctgtatt tccagttcaa tttcttgaac tagtataaaa
    21361 tgtgtgttat aataatcaca taattaaata ataaagacat ttggggtgct ttaggctgag
    21421 atgataccca ttgaacctga tacagttaag actggcgaag ggaaatgtga aatgtttttt
    21481 tcgtatgtcg aaaagaactg tctaatcttg taagctgaac tctaattcgt atctgtaatt
    21541 agagtttttt cttttacgaa actggatgaa gatgatatag tagattgaaa taagatgcga
    21601 acaaatcaat tagaaaattc aaattaattt ctagaaatat tttagcattc ttgtgtactg
    21661 ttctaaattc agtctgctat acaaagtatt taggtcggtc ttcttgagtg ccccactttt
    21721 ctttaaaaaa aggagttttt tatgttgaaa aaatggcagt taaaagatgt tatcttgctt
    21781 gctttcttgt ctatcttttt tggtggggtt ttcgttggtt caggatatgt gtataatatt
    21841 ctcagtctac tcttaacacc tcttggtttg caggcctttg ccaatgaaat cctcttcggt
    21901 ctctggtgta tggctgcgcc cattgctgcc atctttgttc cgagagtcgg aagtgcaacg
    21961 attggagaag tgctagctgc gcttgctgaa gtcctttatg gtagccaatt tggtctagga
    22021 gctcttttgt ctggcttggt tcaaggtttg ggaagtgaat ttggttttat cgtaactaag
    22081 aatcgctatg aaagttggct ctctctaact gctaatagta ttgggattac gcttgttagc
    22141 tttgtctatg aatacattaa gttaggttac tacgcctttt cccttccgtt tgtcctttcc
    22201 ttacttgtgg tacgttttat ttctgtttat ttcttctgta ccatcttggt tcgtgccatt
    22261 gtcaaactct atcatcagtt tgcaactgga ggaaaagcat agatgggtct ggaactacga
    22321 gcgattcagt ccccaatctt ctctgagccg tttgatttta cttttcatgc gcaagccttt
    22381 accttgttag ttgggagtag tgggtctgga aaatccagcc tctttcaaat gattgcccaa
    22441 gtcagttctc ttccctatag tggtcaagtc ctgatagatg ggagcgaggt cagtcaactt
    22501 tctatcatcg cacgtgtcca gaaggttggc attctctttc aaaatcccaa tcatcaattt
    22561 accatggaga gcttgtttga ggagctgatt ttcaccttgg agaatatcgg ctatcacctt
    22621 caggaaattg attctaaaat agcagaggtt gtccagcaat gtcgttgcga ggcaatctta
    22681 caccgtccca ttcatcacct atcaggtggg gaaaagcaaa aagcagcgct ggctgtcctc
    22741 tttgccatga atcctagggt ctatctcttg gatgaacctt ttgcttccat tgaccgcaag
    22801 agcagaatcg agatattgga gattctaaaa gagttggctc ttgatggaaa gacagttatt
    22861 ttgtgcgacc atgatttatc tgactataaa gcctatatcg accatatggt tgagctaaga
    22921 gacggaaaac taagggaagt gtttcaaatc ccttcctatg agatgacaca ggttgcttca
    22981 aaggaagttg cttctagccc ggaactattc catatggata gaacgacttg tgagtttgga
    23041 aatcgccccc tcttttcaat tgctgatttc acattctatc aagggatttc ctgtatcctg
    23101 ggtgacaatg gtgtcgggaa atcaaccctc tttcggtcta ttcttcaatt tcaaaagtat
    23161 aaggggcgca ttgcatggaa gggttcggtc ctgaaaaaga aaaagagttt gtatcgtgat
    23221 ctgactggtg ttgttcagga agctgagaag cagtttatcc gagtcagtct gcgagaggag
    23281 cttcaattag atggacctga ttctgaaaga aatcagcgga tttttcaagc tttacgatat
    23341 tttgatttgg agcaggcagt cgataagagt ccctatcaat taagtggtgg tcagcaaaaa
    23401 attcttcagc tcctgaccat cttgaccagt aaggcttccg tgatcttgct agatgaacct
    23461 tttgcaggtt tggatgatag agcctgccat tatttttgca agtggattgt ggaggagagg
    23521 aatcaaggaa gaagttttct gctcattagt catcgtttag accctttgat ttctgtggtt
    23581 gattattgga ttgagatgac tagtcagggt cttcggcatg tgaaagaagt gaccattacc
    23641 aaaccactta catctcagag tagcaatacc caaggggagg tgagatagta tggtcaaagt
    23701 agcaacccag acaccgatta tcagtctctt cttgctgatt ttatccttgg aaacatcttt
    23761 cattccttcg attgctctga atctttcggt agtcgcattt tgtattctct ttatgctcta
    23821 ttaccgtcga tttaaaatgt tagcttggat gatcatactt gccattttac catcttttgc
    23881 caactactgg gcagttcagt tacacggaga tgcttcacag gcagtcatgc ttggaacgag
    23941 ggcctttgtg acagtttgta tcggccttgt ctttgtttcc tctatttcac taaaagagct
    24001 tctcttgtac ttggctcaaa aggggctatc acgctcttgg tcctatgcct tgattgtggt
    24061 attcaattct tttcctctca ttcagcaaga aatcaagtcc ctcaaagaag cttgcctatt
    24121 acgtggtcaa gaactacatt tttggtcgcc cttgatttac agtaaggttc tgatgacagt
    24181 ctttaggtgg cgccatcttt acctgagagc tctatctgct cacggatatg acgaacatgc
    24241 acagttgaag aatagctatc ggacttttta tattcctaaa aaaacaaaat taatctacct
    24301 gcttttcttt ttattgcttc aaaccagtct atttttataa aggagttatt atggaattta
    24361 cagatattgc gatggaatta tccaagaaag cttggcaggc ttcctttcat catcccttta
    24421 ttttacaatt acaagagggg aatttagaac ctgccatttt ccgctattac ctgattcagg
    24481 atgcctacta tctgaaggct ttctcagaaa tctatcatct tttggctgat aagacttcaa
    24541 accaagagat gaaaagactc ttgaaacaaa atgctcaggg tttagtggag ggtgagttat
    24601 ttattcgcca acaatttttc aaggaattgg aaatcagcga ccaggaaatg gagcaacatc
    24661 caatcgctcc aacctgttat cattacattt ctcatattta taggcaattt gcagaaccga
    24721 acttagccat cgcttttgca agcttgcttc cttgtccttg gttataccat gatataggca
    24781 aatcacttaa tcttaaacca tcaccaaatc ctctctatca acaatggatt gaaacttata
    24841 ttacggatga gttagagcaa cagatcagag aggagggagc actggtcaat cagctctatc
    24901 gagaaagtga tgagacagat aagcaaaaaa tgctagatgc cttccacatc agtgttcata
    24961 tggaagccaa gttttgggag atggcttacc aacaccagac atggaagagc gatttacagt
    25021 ctttagaaaa aggagaagaa tagaaacatg agaaagcacc aactacaagt tcacaaattg
    25081 accattttat ctatgatgat tgcccttgat gtagtcctta cacctatctt tcggattgag
    25141 ggaatggcac cgatgtccag tgtagtcaat attctagcgg gaatcatgat gggacctgtt
    25201 tatgccttgg ctatggctac agtgacagcc tttatccgta tgacgactca agggattccg
    25261 cctttagctc tcacaggagc gacttttgga gcccttctag caggtctctt ttataagtac
    25321 ggtcgaaaat tttacttttc tgccttggga gaaattgtgg gaacaggtat tattggttct
    25381 attgtttcct atcctgttat ggtactcttt acaggatcag ctgctaagct tagctggttt
    25441 atctacacgc ctcgattttt cggagcaacc ttgattggta cagcgatttc ctttattgcc
    25501 tttcgatttt taatcaagca ggaattcttt aaaaaagtgc agggatattt ctttagtgaa
    25561 aggatagact gatgcaggaa tttacaaatc cctttcctat aggctctagt tccctcattc
    25621 actgtatgac caatgagatt tcttgtgaga tgctggcaaa tgggattttg gctctgggat
    25681 gcaaacctgt catggcagat gatccccgtg aggttcttga ttttactaag caaagtcagg
    25741 ctctcttcat caatttgggg catttgtcag ctgagaagga gaaagcaatc cgtatagcag
    25801 cttcttatgc agctcaagtt tgtctcccaa tggtagtaga tgcggttggc gtaacagctt
    25861 catccattcg taagagctta gtcagagacc ttttagacta tagacctacg gtccttaaag
    25921 gaaacatgtc agaaattcga agtcttgttg gattaaagca ccacggcgtt ggggtcgatg
    25981 cgagtgctaa agatcaagaa acggaggatt tgcttcaagt cttgaaagac tggtgtcaga
    26041 cctatcatgg tatgtctttc ttagtcacag gtcccaagga cctcgtcgtt tcgaaaaatc
    26101 aggtcgctgt actgggaaat ggctgtgcag aattagactg gataacaggg acaggagatt
    26161 tggttggagc cttaacagcc gtttttctca gccaaggaaa gactggtttt gaagcttctt
    26221 gcttagcagt ctcttatctc aatatcgctg ctgagaaaat agttgttcaa ggaatgggat
    26281 tggaagaatt tcgttaccaa gtactcaatc agctttcgct cctaagaaga gatgaaaatt
    26341 ggctagatac catcaaagga gaggtttatg aatagagaag cacttagact atatctggta
    26401 accaatcgct atcaagattc cgtggaaagc tttcttgcaa aagttgagac ggcctgccgt
    26461 tcaggggtta ccatagtcca attgcgagaa aaaaatctca caaccaatca atattatcaa
    26521 ctggcaaaac aagtcaagga aataacagat gcttatcagg tacccttgat aatcgatgat
    26581 cggttggatg tttgtcttgc ggttgatgct gctggtctgc atattggaga tgacgaactc
    26641 ccagtttcgg ttgcccgaca agtcttgggt cctgaaaaaa tcctcggtgt caccgctaaa
    26701 acggttaaaa gagctctgga agcggagaaa tcaggtgcag attacttggg gacaggagcc
    26761 atttttccga caaccaccaa ggaaaatgca cccatcaccc tgatttcaac cttgaaaaca
    26821 atttgccaaa cggttgccat tccaatagtt gctattggtg gcttgacttc ggagaatatt
    26881 gatcagctta tgggcactgg catagctggt gtagctgttg tacgtgattt gatgcaagca
    26941 gaagatattg aggcaaaaac gcaagccttt ttaacaaagt tgcatgacat tctttcctaa
    27001 ttaatactca atgaaaatca aagagtaaac tagaaagcta gtcgcaggtt gctcaaagta
    27061 ctgccttgag gttttagata agactgacga agtcagctca aaacagtgtt ttgaagttgc
    27121 agatggaagc tgacgtagtt tgaagagatt ttcgaagagt ataaaaactc tctaattccc
    27181 ttaaagtggg aactagagag ttttttttct taatctttaa aagttgtatg gttgactgga
    27241 ccagaaccat gaccgagttg aggggcatct tgaatagctt ttgtgataaa ggccttggcc
    27301 ttatcaactg cctggtaaag actcttgccc ttggctagtt cagcagtaat cactgcagca
    27361 aaggtacatc cagtaccatg ggtgtgacag gtttgaattc gtgggctttc ccagacaaat
    27421 tgttcattct tggtaaagag gaaatcttta gcaccacctt tgagatgtcc gcctttgata
    27481 accacagact gaggaccaaa ttcttttaaa atcaggcgac cagcacgctg catgtcttcg
    27541 gggtcatgga ttgaaaaacc aacaatctct tctgcttcag gaagatttgg cgtaataata
    27601 gttgctagag gtagtaagtt tgttttgaga tagtctctag cacttgagtc aatcaaggca
    27661 tctccacttg tagcaaccat aacaggatca aggacatagg gacaatccag ttttttaaga
    27721 tagggttgga tgatttccat gatttcagta gtagccaaca ttccagtttt tacagcctga
    27781 ggtggaatat cagaaaagac actctccaat tgggctttca acatttgagg agaaacgtgc
    27841 tcgattagct gaacacctct ggtattttga gcgacaagac tggttacaac agccattcca
    27901 tagacatctc tcgcttggaa tgactttaaa tctgccataa tgccagcacc accactaggg
    27961 tcagtccctg caatggtcaa agcaacgggt aaataagtca tctaaaacct cccaaccaac
    28021 tgaagtttgt attcttaaga acaagctagt ctgttttaga aaggcaatag aagactgcta
    28081 gtccccatta tcatttccac ttccatcagc tagcattacc tagattgagt cagagggatc
    28141 taacagtcgt taatctcagc tttacgcacc cctagtggtt gttttctttt ttctttagta
    28201 tagcatattt ttattgttta tatagtaata tttgactgca aatcgttttt caaatgatta
    28261 aaattctatt tttcgaagaa aatattattt ctataattga caaatgtaga ttttgggagt
    28321 atactgttga gtgtaattga caaatgtaga ttttggaggt atggtaatgc agatttcaga
    28381 tgcagaatgg caggtcatga agattatttg gatgcagggg gagcagacca gtacagattt
    28441 gattagggtt ttggcagagc ggtttgactg gtccaagtcc acggttcaga cacttttggc
    28501 tcgtctagtt gagaaagagt gtttgactcg gaaaaaagaa ggcaagtcct ttgtttattc
    28561 agcgctttta actctagacc aaagtcggga tttacttgtc caagatatca aagacaaggt
    28621 ttgttcccgt aggattagga atttgttggc tgacttgatt gtagaatgtg aatttactca
    28681 gactgacttg gaagacttgg aagctgtgat ttcagagaag aaatcaagtg ctgtaacaga
    28741 agtaagatgt aattgtatgt aaaggagacg tcatgttaaa tagtattgta accattattt
    28801 gtattgccct tatcgcgttt atcttgtttt ggtttttcaa aaagcctgaa aaatctggac
    28861 aaaaagccca gcaaaaaaac ggataccaag agattcgagt ggaagtcatg ggaggctata
    28921 ctcctgagtt gattgtcctc aagaaatcag tgccagcccg cattgtcttt gaccgcaagg
    28981 atccttcacc atgtctggat caaattgttt ttccagattt tggtgtacat gcgaacctgc
    29041 caatggggga agagtatgta gtggaaatca cgcctgaaca ggctggagag tttagctttg
    29101 cttgtggtat gaacatgatg cacggcaaga tgattgtaga gtaggtggag actatgacag
    29161 aaattgtgaa agcaagctta gaaaatggca ttcaaaaaat ccgtatccaa gctgaaaaag
    29221 gctatcatcc agcccatatc cagcttcaaa agggaattcc agctgagatt acctttcatc
    29281 gtgctactcc ttcaaactgt tataaggaaa ttctgtttga agaagaaggt atcttggaac
    29341 caatcggcgt agatgaggag aaagtcattc gttttacacc tcaagaatta gggcgacatg
    29401 aattttcttg tggcatgaag atgcaaaagg gaagctatac agtcgttgag aagactcgaa
    29461 aatctctatc tctcctgcaa cgtttttgga ttactagtat ctgtactgtg cctcttgtga
    29521 ttctcatgat tgggatgttg gcaggtagca ttagtcatca agtcatgcat tggggaacct
    29581 ttctagcaac aacgcctatt atgttagttg caggtaagcc atatatccag agtgcttggg
    29641 ccagttttaa aaagcacaat gccaacatgg ataccttggt tgcgctggga actctagtgg
    29701 cttatttcta tagcctagtt gctctctttg ctggtctccc tgtttacttc gaaagtgctg
    29761 gatttatcct ctttttcgtt cttttgggag cagtttttga ggaaaaaatg agaaaaaata
    29821 cgtcccaagc tgtggagaaa ttactggact tgcaagctaa aaccgcagaa gtcttgagtg
    29881 atgatagtta tgtccaagtt cctttggaac aagtcaaggt aggcgacctg attcgagtgc
    29941 gtcccggtga aaagattgct gttgatggtg tcgtagtaga aggtgtctct agtattgacg
    30001 aatccatggt gacaggtgag agtctgcctg tggacaagac agttggagat actgtcattg
    30061 gctcaaccat caatcatagt ggaacgcttg tctttagagc agaaaaagtt ggctcagaga
    30121 ctgttttggc tcagattgtg gattttgtga agaaagctca gacaagtcgt gcgccgattc
    30181 aggacttgac ggataagatt tcagggattt ttgtcccagt agttgtcatt ttaggaatca
    30241 tgaccttttg ggtttggttc gtcttgctca gggatagtgt ggttgtgctt ggagctagct
    30301 ttgtgtcctc tcttctctac ggagtggcgg ttttgattat cgcctgtcct tgtgccttgg
    30361 gacttgcaac accgacagcc cttatggtgg ggacaggacg tagtgccaag atgggggttc
    30421 tcctcaaaaa tggaactgtc ttacaggaaa tccagaaagt tcaaaccctt gtctttgata
    30481 agaccgggac tttgacggaa gggaaacctg tggtaacaga tgtcatcggc gacgaagtag
    30541 aagtgtttgg attggcagcc tccttggaag atgcttctca acacccactg gctgaggcta
    30601 tcgttaagcg agcgagtgaa gctggacttg agtttcaaac tgttgaaaat tttcaggcct
    30661 tgcacgggaa aggtgtttca gggcgaatca atggaaaaca agttttactt ggaaatgcta
    30721 aaatgctgga tggcatggat atttctaata cttatcaaga taaactagaa gaactagaaa
    30781 aagaagctaa gacagttgtg ttcttagctg ttgacaatga aatcaaaggc ttgcttgctt
    30841 tgcaagatat tcctaagaaa aatgctaagc tagccatcag tcagctaaaa aaacgtggtc
    30901 tcgggacagt catgctgaca ggagacaatg ctggtgtggc gcgtgctatt gcagatcaaa
    30961 tcggaattga agaggtcatt gcaggcgtct tgccagaaga aaaagcccat gaaatccata
    31021 aactgcaaca gtcaggtaaa gtagcctttg ttggtgatgg gatcaatgac gctcctgccc
    31081 ttagtgtagc agatgtgggg attgctatgg gagctggaac agatatcgcc atcgagtcag
    31141 cagatttggt gttgacaacc aataatcttt taggagtggt gcgtgccttc gacatgagta
    31201 agaaaacctt tcatcgtatt ctgctcaatc ttttctgggc ttttatctac aatgttgtcg
    31261 gaattccgat tgcagcagga gtcttttcag gtgttggact ggctctcaac ccagaattgg
    31321 caggtctagc catggccttt agttctgtat ctgttctgac cagttccctt ctgttaaatt
    31381 ttagtaaaat agactaaaag taatgactag atttttttgt tataaaacag aaatgacgac
    31441 actttcaaaa ctgtattaga atagagaata gagagttttg agcagatttt tagaaaagtc
    31501 agcataaata tgatacagtg gaatagtaaa aaattggaga acgtttccaa ttctatgtaa
    31561 tcgtattctc caagtctaaa aaaattgaag gagagttatc attatgactc aagggaaaat
    31621 tactgcatct gcagcaatgc ttaacgtatt gaaaacatgg ggcgtagata caatctacgg
    31681 tatcccatca ggaacactca gctcattgat ggacgctttg gctgaagaca aagatatccg
    31741 cttcttacaa gttcgccacg aagagacagg tgctcttgca gcggttatgc aagctaaatt
    31801 cggcggctca atcggggttg cagttggttc aggtggtcca ggtgcgactc acttgattaa
    31861 cggtgtttac gatgcagcta tggataacac tccattccta gcaatccttg gatcacgtcc
    31921 agttaacgaa ttgaacatgg atgctttcca agagcttaac caaaacccaa tgtacaacgg
    31981 tatcgctgtt tacaacaaac gtgtagctta cgctgagcaa ttgccaaaag taattgacga
    32041 agcctgccgt gctgcagttt ctaaaaaagg tccagctgtt gttgaaattc cagtaaactt
    32101 cggtttccaa gaaatcgacg aaaactcata ctacggttca ggttcatacg aacgctcatt
    32161 catcgctcct gctttgaacg aagttgaaat cgacaaagct gttgaaatct tgaacaatgc
    32221 tgaacgccca gttatctatg ctggatttgg tggtgttaaa gctggtgaag tgattactga
    32281 attgtcacgt aaaatcaaag caccaatcat cacaactggt aaaaactttg aagctttcga
    32341 atggaactat gaaggtttga caggttctgc ttatcgtgtt ggttggaaac cagccaacga
    32401 agtggtcttt gaagcagaca cagttctttt ccttggttca aacttcccat ttgctgaagt
    32461 ttacgaagca ttcaagaaca ctgaaaaatt catccaagtt gatatcgacc cttacaaact
    32521 tggtaaacgt catgcccttg acgcttcaat ccttggtgat gctggtcaag cagctaaagc
    32581 tatccttgac aaagtaaacc cagttgaatc aactccatgg tggcgtgcaa acgttaagaa
    32641 caaccaaaac tggcgtgatt acatgaacaa actcgaaggt aaaactgagg gtgaattgca
    32701 attgtatcaa gtttacaatg caatcaacaa acatgctgat caagacgcta tctactcaat
    32761 cgacgtaggt aacactactc aaacatctac tcgtcacctt cacatgacac ctaagaacat
    32821 gtggcgtaca tctccactct ttgcgacaat gggtattgcc cttcctggtg gtatcgctgc
    32881 taagaaagac aatccagatc gccaagtatg gaacatcatg ggtgacggag cattcaacat
    32941 gtgctaccca gacgttatca caaacgttca atacgacctt ccagttatca accttgtctt
    33001 ctcaaatgct gagtacggct tcatcaagaa caaatacgaa gatacaaaca aacacttgtt
    33061 tggtgtagac ttcacaaacg ctgactacgc taaaattgcg gaagctcaag gagctgttgg
    33121 attcacagtt gaccgtatcg aagacatcga tgcagttgtt gcagaagctg ttaaattgaa
    33181 caaagaaggt aaaactgttg tcatcgatgc tcgcatcact caacaccgtc cacttccagt
    33241 agaagtactt gaattggatc caaaacttca ctcagaagaa gctatcaaag ccttcaagga
    33301 aaaatacgaa gcagaagaac tcgtaccatt ccgtctcttc ttggaagaag aaggattgca
    33361 atcacgcgca attaaataat tcctctcgcc gaaaatcaaa tatgaaactt gtagacttta
    33421 ttctttgatt ttcatagagc agaactttaa aagatcggag caatccggtc tttttatgga
    33481 ggagagtaaa tgaatttaaa tcaattagat attatcgttt caaatgttcc ccaagtctgt
    33541 gctgacttgg agcatatttt ggataaaaag gcagattatg ctgatgatgg ttttgctcag
    33601 tttacgattg gtagtcactg ccttatgttg tcacaaaatc atttggttcc tttggaaaac
    33661 tttcagtcag gaatcattat tcatatcgag gttgaggatg tagaccagaa ctacaaacgg
    33721 ttgaacgagc ttggtataaa ggttttacac ggaccaactg taaccgattg gggaacagag
    33781 tccttattag ttcaaggacc tgctggtcta gtgcttgatt tttatcgtat gaaataggtc
    33841 gcgtagtcat ttgacgttaa ccttagctat ttttaaaata gaaattgaga atatgaacta
    33901 ttgaaagatc ggagcaatcc ggtcttttta tataaagaaa ttgcatataa tactcatctg
    33961 ctttcaaaaa gcattctagt ccatctccga ttaacgatgg actttatcac ctccttctcc
    34021 agtccttgta tgacatcttg aagttgattc atgacatctt ccaaagttcg aaaggcctta
    34081 ttcttaaatc cacgtttacg aatctctttc cacacttgtt caatgggttc atctctggtg
    34141 tgtatggagg aataaaggta aaaccaatat tagtcggaat ctttaaggta cttgatttat
    34201 gccatatagc attgtccata acgagtaaaa gataatcatc tggataagct tgtgaaagct
    34261 cttctaaaaa ggcgttcatc cactcagtat tacatccacc agctattaag aaaaatgatt
    34321 cgcctgtatg ggcatcaaca gctccataac aatagtgaaa ttctcgtata tagtgactat
    34381 ggacatgtgg acctactcct attggagccc aacaagatcc cagtttactg attctaccga
    34441 aaccagcctc atcttggtac atcaagcgaa ccttacgaaa acgtctactg cttttaaagc
    34501 gctttctttt cttcttgaat tgagatttta tttttagacg cgacaatggt ttgagcgtct
    34561 gctttcctag gatgttctgg acgtggcata atatttcgcc aaccatggtg cttcaacagt
    34621 tgatagaagg catcacgtgt gtaggaacga cctaactcct ttttataagc ctgaaataag
    34681 gcatcaattg taacaaattc tcctgcctct gtagccttca aatggcgggc aagaaagact
    34741 ttcttttcct caatcgtcat atatgcatgg ttacggccac cacgtgtttc ttgaaggaga
    34801 gagtcgagtt cgaactcctc atattttttt acatttcgcc aaatcgttgt ttgattacag
    34861 tctatagtgt attgaatcta taacagtaca ccttagctgc taaaatattt ctataaatta
    34921 gtttgacttt cctgatagag ttgttcacat cttatttcaa actactataa aagttctata
    34981 atctctttat aagatttgcc catcagacga aatataataa attgaaacta gaatagtaca
    35041 cctctacttc taaaacattg ttagaaatcg atttgactgt cctgatcgat ttgccctgtt
    35101 cttgtttcgt tttactatag aacgatttga aggcgtttat gatatttagc tgtacgagag
    35161 tctttaaaaa gtgttttgat ggtttggatt tcttctttag ttgatttcat atcactatta
    35221 tataatgctt tttgatttta gtatagtatt atcgtagaaa atggactcgg tgctatggat
    35281 acaccggatg agaatggtta tgtagcagat gactatcgga ttacttactt agaggcccac
    35341 atcaaggcca tgcgagatgc catttacaaa gacggggttg acttgcttgg ttatacgaca
    35401 tggggctgta tcgattcggt ttcagctggc acaggggaaa tgaataagcg ctatggtttt
    35461 atctatgtag atcgagataa tgtaggaaat gggactctca aacgtagcaa gaagaagtcc
    35521 ttctattggt acatgtcatt tatagcaatg gtgtaagcat cgagtagagc acatttgttt
    35581 actattttta taaaatcttc tccctgcttt ataagatgta aaaaagagtt caattcgaac
    35641 tctttttttg ctataatgag gggagaaaaa tcagacagga gattaagatg tcagaaccat
    35701 tatttttaca atcagttatg caagaaaaaa tctggggtgg taccaagcta cgtgatgagt
    35761 ttggctacga catcccaagt gaaaaagtcg gagagtactg ggctatctca gctcatctaa
    35821 atggtgtttc taaagttgcc aatggtcgtt acgagggaac agatcttgct actttgtatg
    35881 cggaacaccg tgaattattt ggcaatcgtc ctgagcctgt atttccgctt ttgactaaga
    35941 ttttggatgc taacgactgg ctcagcgttc aagttcaccc agacgatgct tatggactag
    36001 agcatgaagg cgaacttgga aaaacagaat gctggtacat catcgctgcg gatgaaggtt
    36061 cagagattat ctacggccac aatgccaagt caaaagaaga actccgccag caaatcgagg
    36121 ataagaactg ggatgcgctg ttgacaaaag tgccagttaa agcaggagat ttcttctatg
    36181 taccaagcgg taccatgcac gctatcgggg cgggtatctt gattcttgaa acccagcagt
    36241 ctagcgatac cacttaccgt gtctatgact ttgaccgtaa ggatgacaag ggcaatttac
    36301 gtgaacttca ccttgaaaaa tctatcgatg tcttgaacat tggtgaaccc gcaaatagcc
    36361 gtccagtgac aatcaaagca gatgatctac gttccactct ccttgtatcc aatgatttct
    36421 tcgcagttta caaatgggaa attactggaa aagttaactt tgaaaagaca gctgactaca
    36481 gcttgttgag tgtcttggct ggtcaaggtc aactaactgt tgacgggaaa aactatccaa
    36541 tccaaaaagg gagacacttt atcctaccaa gtgatgttga agcttggact ctggaagggc
    36601 aagatttaga attaattgtt agtcatccat aaaaaagaaa gggcctgagt tcattactca
    36661 agtccttttt gattacataa attgggcgat gaagaccacg atgatgattg gaatgacgga
    36721 acgaagaaac gaagaaggaa gagccagact tggaacagtc cttgtttcca tgctctttca
    36781 tcgagatgga gttcctccat tgcaagagcc tttttaaaga tatagcctgt aaaaagtgaa
    36841 aggtagagag ctccaaatgg catgaggaga ttgggaacca agaagttcat agcgtcaaag
    36901 aaggtcttac caaaaatgtg aacatccgcc atgacaccgt aagatagggc tgaaggaatg
    36961 ccaaagacaa aggtcaaaat tcctaaaata acactccatt tggcacgttt gctgttatcc
    37021 tggttggtga tattgcccac attgatctcc agcatcacga cagaagaagt gacagtcgca
    37081 aagaggaaga ggacgtagaa aatggttcca aaaggcatct tgtcaaagag ttgaggcaag
    37141 acgataaaga gcaagctcgg tcccccttca gactggatat tgaaggggga ccgagcttgg
    37201 aaaatggcta gacctgccat gatggatatc gagatattca tggctacgat ggagattcct
    37261 gactggacta gattggtttt cttgtccaag taagaagcat aggtcaacat gactgtaacc
    37321 cctagtgaga gggcaaagaa agattgtccc agagcataga ggagaccagc gctagtcagt
    37381 tttgaaaagt ctggtttgag gaagtaaaga accccttcca tggcatttgg caaactgaga
    37441 gagcgaccga tgataacaac aaagatgata aagagcaggg gcatcatgac tttcgaagct
    37501 ctttcaatcc ctttttgaac cccacgtgat acaataaaga tatttaataa gataaaggct
    37561 gcttgagctc ctagggcaat ggctggattt gaaatgattg aagtaaataa ctgagcataa
    37621 tcacccgttc caccaagttg gaacaatttc ccaaactcaa tacctagata gactagaatc
    37681 catcctccga taacactgta aaaagataag aggataaaga gggcaaaggc gccaatccaa
    37741 ccgataaagt tgtacttgtt attcttgccc agttttccaa aggttttgat agcggaaacg
    37801 ccagcactac ggccaagggc aaactcagcc agcaggagag ggaaaccgat taaaatagtg
    37861 gaaatgagaa agattagtaa aaagcctcca ccgccattag cagcagtcat gtagggaaac
    37921 ttccaaacgg aaccaagccc gatgaccgag ccagcagatg ctagaataaa gccaagtttc
    37981 gacccccatt gtgatttttc tgacatagtt aaactctgat ctcctttttc aaaataagaa
    38041 aaagctactt gagttgtcaa atagccttct gtttgattga tagacttgac tatcctatta
    38101 tgttttctaa ggtctgtcaa gaaaacaatt ttcaagtttt cacacctttc tcaaaaacgc
    38161 ttgactctga cctaagggga ggggttatac tatcattgta aggaggaaat catgtaccat
    38221 ataaaagaag ctgcgcagct ttcaggtgtc tctgtcaaga ccctgccctg catcactatg
    38281 acaagatagg actcttggtc cccttaaagt cggaaaacgg ctatcgaacc tatagtcaag
    38341 aggatttgga acgccttcag gtcattcttt actacaaata tctaggcttt tctttagaga
    38401 aaatagcaga actgttaaag gaagaaagga cagatttatt gctccatttg actaggtagt
    38461 tggactatct aactcgcgaa aggcaacatc tggatacctt gatttccacc ttgcaaaaaa
    38521 ctattcaaga acaaaaagga gaaagaaaaa tgaccattga ggaaaaattc acgggattta
    38581 gctatcaaga caatcaaaaa taccaccaag aagcggtaga gaaatatggt caagaagtca
    38641 tgggacaagc gctcgaacgc caaaaaggtc acgaagacga ggctacggcc gcctttaacc
    38701 aagtctttca aactttggca caaaatcttc aagttggttt acctgcaaca gcaaccgaaa
    38761 accaggagca agcagccaag ctcttgcaag ccattcgcac ttatggattt gactgctcta
    38821 ttgaggtatt cggtcatatc ggtaaaggtt acgtctacaa cccagagttt aaggaaaaca
    38881 ttgacaagtt tggttctgaa acagcccagt acacgtcaga tgccattgcg gcttacgttc
    38941 agacaaatgc agaaaaaata ggctaggaat ttcctagcct attttttact tcaaatcata
    39001 aagccagtcg tcaccgtttt tgtagtaaaa gaattcactg agatcttctt ctagaaacac
    39061 acgaagcata tcagacatat catcggttgc aagttttaga tgagaaagat tttcaaagtc
    39121 ctcccaccaa actttccctt cgtctgaaga ctggagttca ccagtaaagt gttctgtctt
    39181 gtaaaaaagg acgacataac gataatcctt gtcgtcatac cagtttttga taccacagag
    39241 ttggggtttg gaaatgatca gaccagtttc ttctttcact tcacgaatga cagcatcgac
    39301 aaaggattcg ccacgttcaa catgaccacc aggaaaagta atgccagacc agtcgggact
    39361 aactcggtct tggaccagga ccttatctcc gtttttaatc atacacatgt taacaaattc
    39421 gactgcctct cttctgttca ttcttcacaa cctttaatct ttaatcataa tgcagacttc
    39481 ccgccaccca gccggtacag agggcagaag tgatgttaaa gccacccgtg tgggcattga
    39541 tatccataac ttcgcctgta aagtggaggc caggtaccag cttactttca agggttttag
    39601 gattgatttc cttgagactg actccaccct tggtaacaaa ggactttgca agggacattt
    39661 ttccagttac aggaatttta agttctttaa tggactggac aagttgttct cgttcctttt
    39721 cagtcagttg tttgactttt tcaggatatc cttgtacaaa aaattcggcc aagcgttctg
    39781 gtaacaaggt ttttaaagcg tttttcaagg atttttcccg attttcttct agaaatgtaa
    39841 ccaagtcctt ctcagaaagt tgaggcaaaa catcgagtga gagaacctcc ccacctttga
    39901 caaagctaga catgcgtagg gcagcaggac ctgacaaacc aaagtgggta aagagtaaat
    39961 catgagtgat gacatgctta ccataactta gggtcacatc gtccagagaa atcccttgta
    40021 aggctttatg tggaaaatct gttaataaag gactttcagc agcctcaaga tcggtgatgg
    40081 tatgcttaaa atggcgagca atctcgtgac caaaaccagt cgaaccagtc gaaggataag
    40141 acttaccacc tgttgtgaca atgagtttct cacaagtgaa ggtttgatcc gctgacttaa
    40201 ggacaaactg gtcatctact tttttaacag aaacgatttc tatttgagta gcaacttgac
    40261 cacctagttc ggtgattttc ttttccaaag cttcgataat agtccgagac ttgtcactgg
    40321 ctggaaagac gcgtccgtgg tcttcgacct taagtttaac accattttct gtaaaaaagt
    40381 tgatgatgtc atgattatcg aactgggaga aaacactgta aagaaagcgt ccgtttccag
    40441 gaattccagc tagcaggttg tctaagctac cattgttggt cacattgcaa cgtcccccac
    40501 cagtcccagc taattttttt ccaagtttcc gatttttttc gatgaggagg gttttctgtc
    40561 cataaaagct actggaaatc gtagccatca taccagcagg tcccccaccg atgacaatag
    40621 tatcaaaatg tttcatagct ctattgtacc acaaaaaaac aagagatgat ggtcacctct
    40681 tgtcaagaat gcaattaatc aatttcatag cccatcagca aaccgccctc ttctgcatag
    40741 aaactgcaga gaccagaggt tggtagaatt ttaatatccg cttgtgggaa ggtttcacgg
    40801 attcgctctg agagctgttg acaacatttt tcgttattgc gttgggccat gacaatacgg
    40861 ccaccagcat atccagcttt tactaactca tcataggcag cttgaactga tttctttgat
    40921 ccccttgctt tttgtagcaa ttcgagagtt ccagtttcac tagcttttcc gaccatacga
    40981 atgttgagaa ggccaacgac cgtaccgata agcttgctca aacggccgtt cttcaccaag
    41041 ttatcgactt tggctaggac aaagagcaac ttagtttttt cttgataggc agtgatagct
    41101 tcaaccactt cttcaaaaga caagccctgg tcaatcaagt cattcaattt ttctacgagt
    41161 aggtcaactt caccaccagc agataaacta tcaatcacat gaatcttagt gtcaggatgg
    41221 tcttctagat aaatattctt tgctagttga gcactattgt gactgccaga aagggtacct
    41281 gtgatggtta ctaggaaaat gtttttggca ccttcaaatg ctcgcaaata gtcatctggg
    41341 cttggacaag ccgattttga agcttctgca gttgcataca tggtttccat catttggtca
    41401 atatcgagac tggcgtcatc aacaaagacc tgatcagcta cttgaatggt taaggggaca
    41461 cttacaaagg ttgtgttaat agctggtgtt ggcagttgac gataatcaca accagagtca
    41521 gcaataattt tccaagtcat agaaattctc catctttgtc agttatacat tgacaaaggt
    41581 tctgtctttt tttacaatta tatcatgaaa acccttgaaa caaaagcatc atccgtctgt
    41641 tgtgacaagt agaaagaaaa tgttatgtct gaacgtagaa tctctgaaaa gtctcttgaa
    41701 aatctcagaa aatcaaacca agaatccaat ttattaacca gagaagccat tgaaacagcc
    41761 ctcttgcaac tcttggaaaa aaaggagctg accaagatta gtatttctga attggtcaaa
    41821 cgtgcaggtg tttcgcgtgc ggccttttat cgcaattatg actccaaaga ggaaatttta
    41881 gagagcgtct ttaaacgaac tgtccacaat attatggaac agctgcatca ttacgattta
    41941 aagacagacc tttatttggt ttgggttcac cttttccggg aggccagaaa ggaagctaga
    42001 gtgattcaat tggccttgga ttaccatctg gaaaaaatct ttgtccaagc catgcaggaa
    42061 tttctagaaa aataccatgg gaaatcaaaa ggtgtcagct cttatcttca ttccttctgg
    42121 agctcagcca tcgtctctgt ccttctaaaa tggatcaagg atggcatgaa ggtaccagct
    42181 gaaaagattg cagatttagg tttaccattt tttaaaaaat agagaaaaag gagaaaagag
    42241 atgactgaaa aaagactggc ctgggatgag tattttgcag cccaagccct actaattgcg
    42301 aatcgttcca cttgtaaacg tgctaaagtg ggcgcgattc tggtaaaaga taataaggtt
    42361 atttccactg gttacaatgg ttcggtgtca gggactgagc attgtattga tcacgaatgt
    42421 ttggtcattg aaggccactg tgttcgcacc cttcacgctg aggtcaatgc tatccttcaa
    42481 ggtgctgagc gtggtgttcc caaaggcttt acagcctatg taacccattt tccttgtctc
    42541 aactgtacaa aacaattgct tcaggtcggc tgcaagcgcg tggtttatat caaccagtac
    42601 cgaatggacg actacgccca atacctttat caagaaaaag ggacagaatt gacccattta
    42661 ccacttgaga cagtacagac agctcttaaa gaggctgatc taatgtaaaa attatcaaaa
    42721 ataaatggct tagaaagatt tttaaaccgt tttttggtat aataagaaga ataaattgaa
    42781 agaaggaatt ccaaaaatgg gaaaaattga agttattaat cacccactga ttcaacacaa
    42841 attgtcaatc ttgcgtcgta cagatacttc tacaaaagct tttcgtgagc tagtagatga
    42901 gattgcaatg ttgatggggt atgaagtact tcgtgatctt ccactagaag atgtggaaat
    42961 cgaaacacca attacaaaaa cagttcaaaa acaattggca ggtaagaaat tggccatcgt
    43021 cccaatcttg cgtgcaggta tcgggatggt tgatggtctc ttgagcttgg ttccagctgc
    43081 taaagttggc cacatcggta tgtaccgtga tgaagaaaca cttcaaccag ttgaatactt
    43141 ggtgaaattg cctgaggaca ttgaccaacg tcaaattttt atagtagacc caatgttggc
    43201 aacaggtggc tcagcaatct tggctgttga ttctcttaaa aaacgtggcg catcaaatat
    43261 caaatttgtc tgccttgtat ctgctccaga gggtgtaaaa gcccttcaag aagctcatcc
    43321 agatgtagaa atctttacag cagccttgga tgaacgtttg aacgaacacg gttatatcgt
    43381 tccaggtctt ggagatgctg gagaccgctt gttcggtaca aaataagatc gaaaagaaga
    43441 atgacttgga aaagtatttc cagtcacgaa aggaggttgg gtttttgttt ctgtctaatg
    43501 aaagcagagc aaaaatttga ccttttttga ccaagatatt ataatagtct tatcttcagt
    43561 catttgacca acaaaattaa aactcaaaag gagaaatgaa tgattcctgt agttattgaa
    43621 caaacaagcc gtggagaacg ttcttacgat atttactcac gtcttctcaa agaccgcatc
    43681 attatgctga caggtccggt tgaagacaat atggctaact ctgttattgc ccaattgctt
    43741 ttcttggatg cccaagatag tacaaaagat atttaccttt atgtcaatac accaggtggt
    43801 tctgtttcag ctggtttggc aatcgtagat accatgaact ttatcaaggc agatgtccaa
    43861 accattgtta tgggaatggc tgcatctatg ggaacagtca tcgcatcaag tggagcaaaa
    43921 ggcaaacgtt tcatgcttcc aaatgctgaa tacatgattc accaaccaat gggcggtaca
    43981 ggtggtggta cccaacaaac tgatatggct atcgctgcag aacacttgct caaaactcgt
    44041 aataccttgg aaaaaatctt ggctgaaaat tcaggtcagt caatggaaaa agtccatgca
    44101 gatgcagaac gtgataactg gatgagcgcc caggaaacac ttgaatatgg ctttattgat
    44161 gaaattatgg ccaacaattc attgaactaa tgatgataga aggcaaactc gactgggttt
    44221 gctttttttg gtataatagg gagagatttc ttagaaagag gatttatcat gtttgaaaaa
    44281 gtcaatcgct ctggcttgat tatctatctt tactataatc gtgatgccaa aaaactgcag
    44341 gattatggag atattaccta tcattccaag aaacatcgtt acttacaact ctatgttcca
    44401 actcaagaag tggagcaatt ggtcggacgc ttgagcaagg aaaaatttat taaaaaagtt
    44461 agggtttgtc atatccaaga gttggaaaca ccctttgtgg gcaatcttta tcgataggaa
    44521 aacgttatca tcgaaaaagt tcaaggaaaa cattgacaat tttctgataa ttcggtatat
    44581 tcttaacaga ctatttaaga aataaggaga caaaaagatg aagaaaaaat ttgccctatc
    44641 gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt ggagaagtga agtctggagc
    44701 agtcaacact gctggtaact cagtagagga aaagacaatt aaaatcgggt ttaactttga
    44761 agaatcaggt tctttagctg catacggaac agctgaacaa aaaggtgccc aattggctgt
    44821 tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa atcgaagtag tcgataaaga
    44881 taataagtct gaaacagctg aggctgcttc agttacaact aaccttgtaa cccaatctaa
    44941 agtatcagca gtcgtaggac ctgcaacatc tggtgcgact gcagctgcgg tagcgaacgc
    45001 tacaaaagca ggtgttccct tgatctcacc aagtgcgact caagatggat tgactaaagg
    45061 tcaagattac ctctttattg gaactttcca agatagcttc caaggaaaaa ttatctcaaa
    45121 ctatgtttct gaaaaattaa atgctaagaa agttgttctt tacactgaca atgccagtga
    45181 ctatgctaaa gggattgcca aatctttccg cgagtcatac aagggtgaaa tcgttgcaga
    45241 tgaaactttc gtagcaggtg acacagactt ccaagcagcc cttacaaaaa tgaaagggaa
    45301 agactttgat gctatcgttg ttcctggtta ctataatgag gctggtaaga ttgtaaacca
    45361 agcgcgtggt atgggaattg acaaaccaat cgttggtggt gatggattca acggtgagga
    45421 gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc tactttatct caggcttctc
    45481 aactactgta gaagtttcag ctaaagctaa agccttcctt gacgcttacc gtgctaagta
    45541 caatgaagag ccttcaacat ttgcagcctt ggcttatgat tcagttcacc ttgtagcaaa
    45601 cgcagcaaaa ggtgctaaaa attcaggtga aatcaaggat aaccttgcta aaacaaaaga
    45661 ttttgaaggt gtaactggtc aaacaagctt cgatgcagac cacaacacag tcaaaactgc
    45721 ttacatgatg accatgaaca atggtaaagt tgaagcagca gaagttgtaa aaccataata
    45781 gaaaaatgtt gaaataggga atgagccttt gactcactcc ctgtttcgat atttaatact
    45841 cttcgaaaat ctcttcaaac tgcgtcaacg tcgccttgga ttat
//
LOCUS       DBEJAQ010000009        44914 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.009, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000009 DBEJAQ010000000
VERSION     DBEJAQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 44914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.009"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(200..1009)
                     /locus_tag="ACL297_000574"
     CDS             complement(200..1009)
                     /locus_tag="ACL297_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGR0070879.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(1011..1814)
                     /locus_tag="ACL297_000575"
     CDS             complement(1011..1814)
                     /locus_tag="ACL297_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGR0070880.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1824..3311)
                     /gene="tacF"
                     /locus_tag="ACL297_000576"
     CDS             complement(1824..3311)
                     /gene="tacF"
                     /locus_tag="ACL297_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HGR0070881.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3647..4354
                     /locus_tag="ACL297_000577"
     CDS             3647..4354
                     /locus_tag="ACL297_000577"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGR0070882.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4359..5381
                     /locus_tag="ACL297_000578"
     CDS             4359..5381
                     /locus_tag="ACL297_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HGR0070883.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5398..6267
                     /gene="licA"
                     /locus_tag="ACL297_000579"
     CDS             5398..6267
                     /gene="licA"
                     /locus_tag="ACL297_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HGR0070884.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6257..7135
                     /locus_tag="ACL297_000580"
     CDS             6257..7135
                     /locus_tag="ACL297_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGR0070885.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            7147..7836
                     /locus_tag="ACL297_000581"
     CDS             7147..7836
                     /locus_tag="ACL297_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGR0070886.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7932..8780
                     /gene="dprA"
                     /locus_tag="ACL297_000582"
     CDS             7932..8780
                     /gene="dprA"
                     /locus_tag="ACL297_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGR0070887.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9669..10712
                     /locus_tag="ACL297_000583"
     CDS             9669..10712
                     /locus_tag="ACL297_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HGR0070888.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10879..12966
                     /gene="topA"
                     /locus_tag="ACL297_000584"
     CDS             10879..12966
                     /gene="topA"
                     /locus_tag="ACL297_000584"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGR0070889.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            13072..13431
                     /locus_tag="ACL297_000585"
     CDS             13072..13431
                     /locus_tag="ACL297_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HGR0070890.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13524..14156
                     /locus_tag="ACL297_000586"
     CDS             13524..14156
                     /locus_tag="ACL297_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGR0070891.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            14200..14856
                     /locus_tag="ACL297_000587"
     CDS             14200..14856
                     /locus_tag="ACL297_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGR0070892.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14926..15969
                     /locus_tag="ACL297_000588"
                     /pseudo
     CDS             14926..15969
                     /locus_tag="ACL297_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
     gene            15981..17018
                     /gene="leuB"
                     /locus_tag="ACL297_000589"
     CDS             15981..17018
                     /gene="leuB"
                     /locus_tag="ACL297_000589"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGR0070893.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            17015..17284
                     /locus_tag="ACL297_000590"
     CDS             17015..17284
                     /locus_tag="ACL297_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGR0070894.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17288..17647
                     /gene="leuD"
                     /locus_tag="ACL297_000591"
     CDS             17288..17647
                     /gene="leuD"
                     /locus_tag="ACL297_000591"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGR0070895.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17809..18432
                     /locus_tag="ACL297_000592"
                     /pseudo
     CDS             17809..18432
                     /locus_tag="ACL297_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899133.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            18598..20529
                     /locus_tag="ACL297_000593"
     CDS             18598..20529
                     /locus_tag="ACL297_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HGR0070896.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSINSRQNLSVNYDSGVSGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            20516..21832
                     /locus_tag="ACL297_000594"
     CDS             20516..21832
                     /locus_tag="ACL297_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGR0070897.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFFHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            22007..22993
                     /locus_tag="ACL297_000595"
     CDS             22007..22993
                     /locus_tag="ACL297_000595"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGR0070898.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCVKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            23057..23128
                     /locus_tag="ACL297_000596"
     tRNA            23057..23128
                     /locus_tag="ACL297_000596"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23090..23092,aa:Arg,seq:ccg)
     gene            23311..24009
                     /gene="rnc"
                     /locus_tag="ACL297_000597"
     CDS             23311..24009
                     /gene="rnc"
                     /locus_tag="ACL297_000597"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGR0070899.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGGFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            24000..27539
                     /gene="smc"
                     /locus_tag="ACL297_000598"
     CDS             24000..27539
                     /gene="smc"
                     /locus_tag="ACL297_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGR0070900.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSHQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            27536..28330
                     /locus_tag="ACL297_000599"
     CDS             27536..28330
                     /locus_tag="ACL297_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGR0070901.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            28330..29148
                     /locus_tag="ACL297_000600"
     CDS             28330..29148
                     /locus_tag="ACL297_000600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGR0070902.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            29152..30429
                     /gene="ftsY"
                     /locus_tag="ACL297_000601"
     CDS             29152..30429
                     /gene="ftsY"
                     /locus_tag="ACL297_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGR0070903.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVIEVSQKEVKEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(30475..31962)
                     /gene="zwf"
                     /locus_tag="ACL297_000602"
     CDS             complement(30475..31962)
                     /gene="zwf"
                     /locus_tag="ACL297_000602"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGR0070904.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWD
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(32111..32851)
                     /locus_tag="ACL297_000603"
     CDS             complement(32111..32851)
                     /locus_tag="ACL297_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGR0070905.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(32851..35016)
                     /locus_tag="ACL297_000604"
     CDS             complement(32851..35016)
                     /locus_tag="ACL297_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGR0070906.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            35189..36142
                     /locus_tag="ACL297_000605"
     CDS             35189..36142
                     /locus_tag="ACL297_000605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGR0070907.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLQQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSSLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            36219..38207
                     /gene="uvrB"
                     /locus_tag="ACL297_000606"
     CDS             36219..38207
                     /gene="uvrB"
                     /locus_tag="ACL297_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGR0070908.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            38217..38780
                     /locus_tag="ACL297_000607"
     CDS             38217..38780
                     /locus_tag="ACL297_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGR0070909.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            38746..39234
                     /locus_tag="ACL297_000608"
     CDS             38746..39234
                     /locus_tag="ACL297_000608"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070910.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQMWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAIGDLKVVGFISYGNFRDETMQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            39308..39775
                     /locus_tag="ACL297_000609"
     CDS             39308..39775
                     /locus_tag="ACL297_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGR0070911.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(39772..40287)
                     /locus_tag="ACL297_000610"
     CDS             complement(39772..40287)
                     /locus_tag="ACL297_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGR0070912.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(40299..40826)
                     /locus_tag="ACL297_000611"
     CDS             complement(40299..40826)
                     /locus_tag="ACL297_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGR0070913.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(41104..41667)
                     /locus_tag="ACL297_000612"
     CDS             complement(41104..41667)
                     /locus_tag="ACL297_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGR0070914.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(41651..42202)
                     /gene="coaC"
                     /locus_tag="ACL297_000613"
     CDS             complement(41651..42202)
                     /gene="coaC"
                     /locus_tag="ACL297_000613"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HGR0070915.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(42214..42903)
                     /gene="coaB"
                     /locus_tag="ACL297_000614"
     CDS             complement(42214..42903)
                     /gene="coaB"
                     /locus_tag="ACL297_000614"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HGR0070916.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            43133..44803
                     /locus_tag="ACL297_000615"
     CDS             43133..44803
                     /locus_tag="ACL297_000615"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGR0070917.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT        13934 a         7876 c         9762 g        13342 t
ORIGIN      
        1 gaattttcta attgatttgt tcgcatctta tttcaatcta ctatagaaaa aagtctttaa
       61 aattataaaa cgcatcatat caaggttttt caaaaacctt gatatgatgc gttttattgt
      121 gggaatatgt atttcatttt ctactaaaat tatgtttttg aataacctct atcttagtag
      181 tttgtataat ccccctcaat cagtttttgc gataagcttt aatgctatga ctataccact
      241 cttgcatttc ttttgatggt ggtgtcatat aatcgccata catctgggtt aaaaattggt
      301 catatttttt gggaacaggc aacatacggc cctcaaactc agttaaaatc agttctttaa
      361 aggtatcaac tgggaagatt tctttcatcc cttccttacc gatcccaact cctccttcat
      421 attgaggaat gttggttaca gcatttttga ctagttgatc aattttcttg taaaagtagc
      481 gaggattgac aaatcggaga gcgtaccagc tacataatct aagaaaatct tttagtttgc
      541 tatcaccgtg aactgctcgt gattttttga tataagctag ttgacgaaga gccacatact
      601 tatagctctt gtcgacaatg ctcaagtctg taaatcgatc aattgggaag acatcgatga
      661 aaaggctggt atcatgacgc ttgtacttaa catggtcttc tataacagta gaagtgtcca
      721 aaatcgatgc gaaattatgg aagtaccaag aagatgtatc gtaggaaaga accttgtagc
      781 gagggtgatt ttcttcttca ataatcttca gtaaacgctc ataatcctca cgataaaggg
      841 aaatatcaat atcatcatcc caaggaatca tacctttgtg gcggatggct ccaagcatgg
      901 ttccataact gagaaaataa ggaatatcat gtttcttaca agtctcatca atatagtcca
      961 gcagggctag ttgaatttct ttaatttctt ttttttctaa atattgcatc ctaatcctcc
     1021 aatttataag cgtgaaattc atgactgtag aagcgttttt cttctggtgg tagggtcata
     1081 taatctccat aaaattgtgt caaaatagta tcaaattttt caggtgcagg aaggcttaaa
     1141 ttctcaaagg gtaaatcgat tgttttatca aaggtaccac ttgggaagac ttccttttcc
     1201 ttaaattttg aagggataaa agccatatac tgcccatttt cacgactata tttttgaatt
     1261 tctttctcga ttttatttgc aaaataacga ggagaaactg gtcgaaggag caaccaaaag
     1321 gctgttcgta tccaatcttt taaaaggcta tccttataga caatattttt atgtttactg
     1381 aaagacagca gtttgaagct ttccagttta taacaagtat caatgacctt aggatcatca
     1441 aagcgatcca tagggaaaat atcgataaag ataccagact cataggtttt tgtatttcga
     1501 gtatcaataa ttttagtcgt actgtcggtt atcttgataa agttgttaaa gtagttctta
     1561 tcagtttcta aggataggag ttcatacttg cttttttcct tttgaaaaat gttaataaat
     1621 cgttggtagt cttctctagg catggataaa tcaatatcgt cgtcccaagg gataaagccc
     1681 tcatgtcgaa ccgccccaat cagagtaccg tagttaataa tatagttgat attgtgcttt
     1741 ttacagagag tatcaatata atccaaaatt tctaattcaa tttgtttggc atcttcaacg
     1801 gttagttgtt tcattttaaa ctcctatgat tttttaaatt tattttttaa ggctagaaca
     1861 tggtttaaaa attcatagaa aatgctatct tttgtgaaga caagtagact aatataagag
     1921 atagctgata gcaagataat caaaccagta ttaatcaaaa atggcaaatt aatgaccata
     1981 tccacaggat acacgaaatt aatcaggaaa taaattgcta caaaggaaag tgaaaagaga
     2041 gagtatcgaa cagtatagct aaagatatgt cccaagtgga tgagttgttt tctatggatg
     2101 aaaatgatat agaaaacaag tagagaagcc tctgataaca tagttgtcag taagtagtat
     2161 tcgggagcca cgatacggtt gaaaaagagg agactattca agcccaaatt gagtagccca
     2221 gcaaagactg tataaactgt gatacgtttt tcatagccat tggtaaagag aatttgggaa
     2281 ccaagaatgg tatctaaggc caggataatc gtacgaaaag cgaagagaga ggtcaagatg
     2341 ccgcctccga tatatttttc actaccgtaa agtaggatgg catttggtcc taaaaccatg
     2401 agtccaaaac tcagtggaat gataaagaag ttaaagattc gactacctct attaaccaga
     2461 gaaacatagg cttctttgtc tcctttcccc agatagtaac tgagacgagg cacactcact
     2521 ccaattgcac ctgttacaac cccagctata acggtcacaa ttcgctgagc tatggtatag
     2581 taactaacgt tgacatcaat ccctgtttta acgaggaaga ggcgatctaa aaaagtgaag
     2641 agcatattgg cattggcaaa gactaacatg gctgtcagag ggagaaagag tggtttaaaa
     2701 tcacttaggt gaatcttaac aagtttgatg tctcttttaa tccaaaaata actaatcagg
     2761 tagttaatca gcgtcgataa actcatcaca agtgtataga caacaatatc gtgttcattt
     2821 ttaacaaata agaaaataga gaccagcatc aggatacgga tgaaggcagt tttgtaaaag
     2881 agaaaactgt aattttccag agcttcattg acccattcga ttgaaaaaat ctgggcaatg
     2941 agttgaatcc ccataacaag gtagaccttt ttgatgattg gattatcagt aaagaagaga
     3001 ggataggcta ggatatagac agcagtggtc aaaatcgtac aagcgatgca caaataaaaa
     3061 agactagaaa aggttctgtt aagatctttt ttgttatcct tgacattact gatagccctt
     3121 aaaccgtagt tatagacacc ataagttgca aagggcaaga aaaatgacaa aatagtgtcg
     3181 actgagttga agtaaccata gtcagttcgg tccaagacac gcgcgacata ggttccagtt
     3241 aggatgggaa aaataatatt caagacacga attcccatgt aagatagagc atttaatttt
     3301 atacttttca ttcaatttac ctcgtttttc attatatcat aaagttagct aataagaaat
     3361 gaagggcagt aagccaagca atcactttga agtttcaaat cttaaatttt aagttttctt
     3421 taaggaaagt atattattct gaaggactct aaaatttcgc agtcatttat tagtaattgc
     3481 tacagaattc ctagtcatta ctagaaatgg actagtttct ttgaataata gaactgcata
     3541 attctcctat tctagaaggg gaggaccagt atttctttta tgataggact agattgtggt
     3601 ataatagaga gaataagttt ttttagtaag acaaaggaga aaatagatga tttatgcagg
     3661 aattcttgcc ggtggaactg gcacacgcat ggggatcagt aacttgccaa aacaattttt
     3721 agagctaggt gatcgaccta ttttgattca tacaattgaa aaatttgtct tggagccaag
     3781 tattgaaaaa attgtagttg gtgttcatgg agactgggtt tctcatgcag aagatcttgt
     3841 agataaatat cttcctcttt ataaggaacg tatcatcatt acaaagggtg gtgctgaccg
     3901 caatacaagt attaagaaaa tcattgaagc cattgatgct tatcgtccgc ttactccaga
     3961 ggatatcgtt gttacccacg attctgttcg tccatttatt acacttcgca tgattcagga
     4021 caatatccaa cttgcccaaa atcatgacgc agtggacaca gtggtagaag cagttgatac
     4081 tatcgttgaa agtaccaatg gtcaatttat tacagatatt ccaaatcgtg ctcaccttta
     4141 tcaaggacaa acacctcaaa cattccgttg caaggacttc atggaccttt atggatctct
     4201 ttctgatgaa gagaaggaaa tcttgacaga tgcatgtaaa atctttgtga tcaaaggaaa
     4261 agatgtggcc ttggccaaag gtgaatactc aaatctgaag attacaaccg taacagattt
     4321 gaagattgca aaaagtatga ttgagaaaga ctagtaaaat gattaatcaa atttatcaac
     4381 taactaagcc taagtttatc aatgtcaaat atcaggaaga ggctattgac caagagaatc
     4441 atatccttat ccgtcccaac tacatggctg tctgtcatgc ggatcagcgt tactatcagg
     4501 gaaaacgtga tcccaagatt ttgaataaaa agcttccaat ggcaatgatt cacgagtcat
     4561 gtggaaccgt catttctgac ccgaccggaa cctacgaggt tggtcaaaaa gttgtcatga
     4621 ttcccaatca gtctcctatg cagagtgatg aagaattcta tgaaaactac atgacaggga
     4681 cccatttctt gtctagtgga tttgatggct ttatgagaga gtttgtttct ctccctaaag
     4741 atcgtgtggt ggcttatgat gctattgaag atacggttgc agccattaca gagtttgtca
     4801 gtgtgggcat gcacgctatg aatcgtctat tgactcttgc tcatagcaag cgggagcgga
     4861 tcgccgttat tggagatgga agtttagctt ttgtggttgc caatattatc aactatactt
     4921 tgccagaatc agagattgtg gttattggtc gtcattggga aaagttggaa ctcttctcat
     4981 ttgccaaaga atgctatatt acggataata ttcctgaaga gttggccttt gaccatgctt
     5041 ttgaatgttg tggtggtgat ggtactggac cagctattaa tgacttgatt cgctacattc
     5101 gtcctcaggg aacgattctc atgatgggag ttagcgaata taaagtcaat ctcaatactc
     5161 gcgatgcctt agaaaagggc ttgattttgg ttgggtcatc tcgttctggt cgcattgatt
     5221 ttaaaaatgc tatccaaatg atggaagtca agaaatttgc caatcgtctt aaaaatatcc
     5281 tttatctaga agaacctgta agagaaatta aagatattca tcgtgtcttt gcaaccgatt
     5341 taaacacagc ctttaaaaca gtgtttaagt gggaagtata agtactggag gttaattgtg
     5401 gagaaaatca ttaaagaaaa aatttcttcc ttacttagtc aagaagagga agtcctcagt
     5461 gttgaacaac tgggtggaat gaccaatcaa aactatttgg ccaaaacaac aaataagcaa
     5521 tacattgtta aattctttgg taaagggaca gaaaagctta tcaatcgaca agatgaaaag
     5581 tacaatcttg aactactaaa ggatttaggc ttagatgtaa aaaattatct ttttgatatt
     5641 gaagctggta tcaaagtaaa tgagtatatc gaatctgcga ttacgcttga ttcaacgtca
     5701 atcaagacca agttcgacaa aattgctcca atattacaaa ctattcatac gtctgctaag
     5761 gaattaagag gagaatttgc tccttttgaa gaaatcaaaa aatacgaatc cttgattgaa
     5821 gaacaaattc cttatgccaa ctatgaatct gttagaaatg cagtcttctc cttagagaaa
     5881 agactggctg acttaggtgt tgacagaaaa tcttgtcata tcgatttggt gcctgaaaac
     5941 tttatcgaat cacctcaagg acgactttat ttgattgact gggaatattc atcaatgaat
     6001 gatccaatgt gggatttggc tgccctcttt ttagagtctg aattcacttc ccaagaggaa
     6061 gaaactttct tatctcacta tgagagtgac caaacaccgg tttctcatga aaagattgct
     6121 atttataaaa ttttacaaga tactatttgg agtctatgga ctgtctataa ggaagagcaa
     6181 ggtgaagatt ttggtgacta tggtgtgaat cgttaccaaa gagctgttaa aggtttggct
     6241 tcttatggag gttcagatga aaagtaaaaa cggagttcct tttggccttc tctcaggtat
     6301 tttctggggc ttgggtctaa cggttagtgc ttatatcttt tcgattttta cagatttgtc
     6361 accctttgtg gtggctgcag ctcatgattt tttgagcatc tttatcttac tagcttttct
     6421 cttggtaaaa gaagggaaag ttcgcctctc aattttctta aatattcgca atgtcagtgt
     6481 tatcatcgga gccttgctag caggccctat cggtatgcag gccaatcttt atgcagttaa
     6541 gtatatcgga agttctttag cttcatctgt atcggctatt taccctgcga tttcagttct
     6601 attggctttc ttctttttga agcacaagat ttcgaaaaat actgtatttg ggattgtctt
     6661 gattattgga gggattattg ctcagaccta taaggttgaa caggttaatt ctttctacat
     6721 tgggattctt tgtgctttgg tttgtgctat tgcatgggga agtgagagtg ttcttagctc
     6781 ttttgccatg gaaagtgaat tgagtgaaat cgaagccctc ttaatccgtc aagtaacttc
     6841 gttcttgtcc tatcttgtga ttgtgctctt ctctcatcag tcatttactg cagtagccaa
     6901 tggacaattg ctaggtctca tgattgtttt tgcagccttt gatatgattt cctacttggc
     6961 ttattatatc gctatcaatc gcttgcaacc agccaaggct acaggcttga acgtgagcta
     7021 tgtagtatgg acggtcttgt ttgcagttgt tttcttgggt gcaccgctag atatgctgac
     7081 cattatgacg tcacttgtcg tcattgctgg agtttatatt attattaaag aataaaggag
     7141 attcgtgtga aagccattat cttagcagcg ggattgggaa ctcgcttgcg tcctatgact
     7201 gaaaataccc ctaaagcctt ggttcaggtt aatcaaaaac ctttgattga gtaccaaatt
     7261 gagtttctca aagaaaaagg aatcaatgac atcatcatca ttgttggtta tcttaaagaa
     7321 caattcgatt acttgaaaga gaaatacggt gttcgtctcg ttttcaatga taaatacgct
     7381 gactacaata acttttactc tctctatctt gtaaaagaag aattggccaa cagctatgtt
     7441 attgatgctg acaattatct ctttaaaaat atgttccgca atgatttgac acgttcgact
     7501 tattttagtg tttatcgtga agattgtacc aacgaatggt tcttggttta tggagatgac
     7561 tacaaggttc aagacattat tgttgatagc aaggcaggtc gcatccttag tggtgtatcc
     7621 ttctgggatg ctccaactgc agaaaagatt gtcagcttta tcgacaaggc ttatgtaagt
     7681 ggtgaatttg ttgatctcta ttgggacaat atggttaagg ataatatcaa agagctagat
     7741 gtctatgttg aagaattaga aggcaatagc atttatgaga tcgatagtgt ccaagactat
     7801 cgtaaattag aagaaattct taaaaacgaa aattaaagat tccaacatct gacaaaatag
     7861 tcggatgttt tttgattttt tacgaacttt tacgaataga tagatgagta gaaaaagaaa
     7921 tggagttatt tatgaaaatc acaaactatg aaatctataa gttaaaaaaa tcaggtttga
     7981 ccaatcaaca gattttgaaa gtgctagaat acggtgaaaa tgttgatcag gagcttttgt
     8041 tgggtgatat tgcagatatc tcaggttgcc gtaatccagc cgtttttatg gaacgttatt
     8101 ttcagataga cgatgcgcat ttgtcgaaag agtttcaaaa atttccatct ttctctattt
     8161 tagatgactg ttatccttgg gatttgagtg aaatatatga tgcgcctgta cttttatttt
     8221 acaagggaaa tcttgacctc ctgaaattcc cgaaggtagc ggtcgtgggc agtcgtgctt
     8281 gtagcaaaca gggagctaag tcagttgaaa aagtcattca aggcttggaa aatgaactgg
     8341 ttattgtcag tggtctggcc aagggcattg acacagcagc tcatatggca gctcttcaga
     8401 atggcggaaa aaccattgca gtgattggaa caggactgga tgtgttttat cctaaagcca
     8461 ataaacgctt gcaagactac atcggcaatg accatctggt tctaagtgaa tatggacctg
     8521 gtgaacaacc tctgaaattt cattttcctg cccgtaatcg catcattgct ggactttgtc
     8581 gtggtgtgat tgtagcagag gctaagatgc gttcaggtag tctcattacg tgtgagcgag
     8641 caatggaaga aggacgcgat gtctttgcta ttcctggtag cattttagat ggactatcag
     8701 acggttgcca tcatttgatt caagaaggag caaaattagt caccagtggg caagatgttc
     8761 ttgcggaatt tgaattttaa aaatgaccta agctagaatt ctaagaaaaa atcaatttta
     8821 agagaaaatg aacccaacat ttccataata aaacgcatat tagcaagttt ttaacacttg
     8881 ataatatgcg ttttttctaa gtggattagt agagtagagg atttttctca tataatactc
     8941 ttcgaaaatc tcttcaaact acgtcagctt ccatctgcaa cctcaaaaca gtattttgag
     9001 ctgacttcgt cagtcttatc tacaacctca aagcagtgct ttgagcaacc tgtggctagc
     9061 ttcctagttt gcgctttgat tttcattgag tataagggaa agtatagtga attgaaataa
     9121 gatgtgaaca actctatcag gaaagtcaaa ttgatttata gaaatatttt agcagccaag
     9181 gtgcactgtt atagattcaa tacactataa tttagtgtaa ttgagaaagg agaaatgatt
     9241 gtgattgatg ttggctaggt tatgttcaat gattcctacc gtctcaaatc ttgtcagtaa
     9301 ggaaaaataa attcttcaaa agtagagatt acaaggcttg tttaagaaag aattcaaaga
     9361 ccttgacaaa taaaaataaa atggttatta taaaaaatgg tctgaaatag atgatgatac
     9421 ttttcgaaaa tctcttcaaa ctacgtcagc tcagctttgc cttgctgtgt tttgagcaag
     9481 ctacggttag cttccgagtt tgattttcat ttactagaaa tgaaactgat gagagatatc
     9541 agtagacatt tgagtcagga tattatggaa aatgataaaa agagctcgtg agattggcat
     9601 atcagactac taaagtattg agtttgttag gattttagcg actagttagc tgggaaagga
     9661 agatatttgt gacaaataat aaactgtatt cgttgataga atttagaaat aaaatatatg
     9721 aagaattaga actttccaga agtgatttag cgattttact atgtgccatg cttatcgcct
     9781 ctatcggatt aaatatggat tcgactcccg tgattattgg agccatgtta atctctcctt
     9841 tgatgacacc tattctggga gtggggctct ctctagctat atttgatttt aaattgttaa
     9901 gaaaatcttt taaaatatta gctattcaaa ttcttgccag tctaatagct tcaacacttt
     9961 atttttatct ttctcccatt tcgtatgcta gttcggagat tgttgctaga acctctccga
    10021 ctatttggga tgttctcatt gcttttgtag gagggatagc aggtattatt ggtgctagga
    10081 aaaaagagac caataatatt gttcctggtg ttgctattgc aaccgccttg atgcctcctc
    10141 tttgtacagt aggttatgct attgcttctg ctaatctaaa atttatcata ggctcctctt
    10201 acctattcct catcaattgt agctttattg tcattgcgac ttatataggt gttaggttga
    10261 tgatggttaa gaaacattat tttaaagata atgaagaaga ctctaaaatg cgtaggattt
    10321 tgcttctagt tgctgttttg ctgatgattc cgagtttcat ctctgcaacg actttagtga
    10381 gagaaacgtt gaaaaaagag tcccttaaga aatttatatc agagcagttt caggggtata
    10441 atattttgaa aaaaacctat tctaaaaaga ctcataccct aaagctaacc atttcaggaa
    10501 attatttgac agaagaagaa ctcgatatga tttcaagtaa gagaggtgac tatggtttaa
    10561 gtgatgtttc tgttcaagtt tcacaattgt ctgattcaga acaacttagt aaggaagagc
    10621 tggtggagta tttcttccag tatatcaagg ataaggaagc aaaagaaaag gagaaagcca
    10681 ataaatttga tacagagtct gaggagcaat aatttcttga gaatagctgg tttctctcgt
    10741 gagtcttcta tgtatatcaa aggaagactg aggttttaag tataaaactt ttcttctatt
    10801 atagtagcta agtcttgaca gggttcaaaa aatggtttac actttataaa gtttattact
    10861 ttgaaaaggt gtgatactgt ggctacggca acaaaaaaga aaaaatcaac agttaaaaaa
    10921 aatctagtca tcgtggagtc gcctgctaag gccaagacga ttgaaaaata tctaggcaga
    10981 aactacaagg ttttagccag tgtcgggcat atccgtgatt tgaagaaatc cagtatgtcc
    11041 gtcgatattg aaaataatta tgaaccgcaa tatattaata tccgaggaaa aggccctctt
    11101 atcaatgact tgaaaaaaga agctaaaaaa gctaataaag tttttctcgc gagtgacccg
    11161 gaccgtgaag gagaagcgat ttcttggcat ttggcccata ttctcaactt ggatgaaaat
    11221 gatgccaacc gtgtggtctt caatgaaatc accaaggatg cagtcaaaaa tgcttttaaa
    11281 gaacctcgta agatcgatat ggacttggtc gatgcccaac aagctcgtcg gatcttggat
    11341 cgcttggtag ggtattcgat ttcgcctatt ttgtggaaga aggtcaagaa gggcttgtca
    11401 gcaggtcgcg ttcagtccat tgcccttaaa ctcatcattg accgtgaaaa tgaaatcaat
    11461 gccttccagc cagaagaata ctggacagtt gatgctgtct ttaaaaaggg aaccaaacaa
    11521 tttcatgctt ccttctatgg agtagatggt aaaaagatga aactgaccag caataacgaa
    11581 gtcaaggaag tcttgtctcg tctgacgagt aaagactttt cagtagatca ggtggataag
    11641 aaagagcgca agcgcaatgc tcctttaccc tataccactt catctatgca gatggatgct
    11701 gccaataaaa tcaatttccg tactcgaaaa accatgatgg ttgcccaaca gctctatgaa
    11761 ggaattaata tcggttctgg tgttcaaggt ttgattacct atatgcgtac cgattcgact
    11821 cgtatcagtc ctgtagcgca aaatgaggcg gcaagcttca ttacggatcg ttttggtagc
    11881 aagtattcta agcacggtag caaggtcaaa aacgcatcag gtgctcagga tgcccatgag
    11941 gctattcgtc cgtcaagtgt ctttaataca ccagaaagca tcgctaagta tctggacaag
    12001 gatcagctta agctatatac ccttatctgg aatcgttttg tggctagcca gatgacagcg
    12061 gccgtttttg ataccatggc tgttaaattg tctcaaaaag gggttcaatt tgctgccaat
    12121 ggtagtcagg ttaagtttga tggttatctt gccatttata atgattctga caagaataag
    12181 atgttaccgg acatggttgt tggagatgtg gtcaaacagg tcaatagcaa accagagcaa
    12241 catttcaccc aaccgcctgc ccgttattct gaagcaacac tgattaaaac cttagaggaa
    12301 aatggggttg gacgtccatc aacctatgcg ccaaccattg aaaccattca gaaacgttat
    12361 tatgttcgcc tggcagccaa acgttttgaa ccgacagagt tgggagaaat tgtcaataag
    12421 ctcatcgttg aatatttccc agatatcgta aacgtgacct tcacagctga aatggaaggt
    12481 aaactggatg atgtcgaagt tggaaaagag cagtggcgac gggtcattga tgccttttac
    12541 aaaccattct ctaaagaagt tgccaaggct gaagaagaaa tggaaaaaat ccagattaag
    12601 gatgaaccag ctggatttga ctgtgaagtg tgtggcagtc caatggtcat taaacttggt
    12661 cgttttggta aattctacgc ttgtagcaat ttcccagatt gccgtcatac ccaagcaatc
    12721 gtgaaagaga ttggtgttga gtgtccaagc tgtcatcagg gacaaattat tgagcgaaaa
    12781 accaagcgta atcgcctctt ctatggttgc aatcgctatc cagaatgtga atttacctct
    12841 tgggacaagc ctgttggtcg tgactgtcca aaatgtggca acttcctcat ggagaaaaaa
    12901 gtccgtggtg gtggcaagca ggttgtttgt agcaaaggcg actacgagga agaaaagatt
    12961 aaataagagg aggggcccga aagtaaattt caggctcctt ttggttagaa cttgacaaaa
    13021 tacatcattt tatgcgaaac tagaagaaga ttattttaac taggagaagt tatgcgtctt
    13081 atctatctga taattggttt tttatcactg gccttggcta ttgttggggt tgttttaccc
    13141 ttgttgccta caacaccttt ccttttgttg tctattgctt gtttctccag aagttccaag
    13201 cgattcgaag actggcttta tcataccaag ctctatcaaa catatgtagc tgattttcgc
    13261 gagactaagt ctattacgcg tgaacgaaag aaaaaaatca tcgtatccat ttacattttg
    13321 atggggattt ctatttattt tgcccctctc ttaccagtca aaatcggtct gggtgctttg
    13381 accatcttta tcacttatta tctcttcaag gtcattccag acaaagaata gttaaaacag
    13441 tagttattta ccttgataaa attgaaagca tattcataac aatatgatat aataaatttg
    13501 aagtaatatt caaggagaat caaatgattt acgaattttg tgctgaaaat gtgactttac
    13561 ttgaaaaagc gatgcaggct ggagctcgtc ggattgaact ctgtgataat ctagcagttg
    13621 gtgggacaac acctagctat ggagtgacta aggcagcggt tgaactggca gctaactacg
    13681 atacaaccat catgaccatg attcggccac gtggtggtga ctttgtctat aatgacctag
    13741 aaattgctat catgctagaa gacatttgtt tgactgctca ggctggaagt caaggggttg
    13801 tatttggagc tttaactgct gataaaaagt tggataagcc taatctggaa aagttaattg
    13861 ctgcatcaaa aggaatggaa attgtctttc acatggcctt tgatgaacta agtgatgaag
    13921 atcaaccgga agctattgac tggctcagtc aagccggtgt cactcgtatc ctaactcgtg
    13981 ctggtgtgtc tggcgactcc ttagaaaaac gttttgttca ctatcacaga attttggagt
    14041 acgctaaagg taaaattgaa attctaccag gtggggggat tgaccttgac aaccgtcaaa
    14101 cctttattga ccaggtgggg gtaacacaat tgcatggtac taaggttgtt ttttaaaaaa
    14161 tagaaaggaa ctgctagcct tgggtagcag ttttcactta tgtttgaaat ttttaaatcc
    14221 tatcaattta atcaagaaaa ggctcatgat tatggtttta tagaaaatag cgaagtctgg
    14281 acatatagtt gccagatttt gcaaggtgac tttgtcatga ctgtgtccat cactgctgat
    14341 aatgtgaact ttcaagtctt tgaccaagag actggtgacc tctatcctca cgttcatatg
    14401 gaaagcatga ggggaagttt tgtcggaaat gtccgtgagg cttgtctgga gattctttac
    14461 cagattcgga aggcttgttt tgatgtgcaa gattttatct gtcatcagac taagcgtatc
    14521 atgactcaag ttcaggaaaa gtatggaaac cagttggagt atctgtggga aaaatcgcct
    14581 gatacagctg tattgcgcca tgaaggcaat caaaagtggt atgccgtctt gatgaaaatc
    14641 tcttggaata agctggaaaa gggcagagaa ggacaagtgg aagcagtcaa cctcaagcat
    14701 gaccaagtag ctaatttgct ttcacaaaag gggatttatc cagccttcca tatgagcaag
    14761 cgctactgga ttagtgtgtc ccttgatgat actttatcag atgaagaagt actggaattg
    14821 atagaaaaaa gttggaactt aacctctaaa aaatgaaata ttttaataat tttcatgaac
    14881 tttcaattag ctaaatattc tttactgaag aaatttttag aaaatatagg atttaccaca
    14941 ctagaggaat atggtgccat cttcaaatac ctgattgaga atgtcaagac ggatcgtcag
    15001 atcatctgtt cgcctcactg tcatgatgac ctcggaatgg cagtggcaaa tagccttgct
    15061 gctgtcaaga atggtgcagg acgtgttgaa gggactatca atggtattag ggagcgagct
    15121 gaaaatgctg ctttggaaga aattgcagtg gctctcaata ttcgccaaga ttactaccaa
    15181 gtagaaacca gtattgtcct aaatgagacc atcaatacgt cagaaatggt ttctcgcttc
    15241 tctggtattc cagttcctaa aaacaaagcc gtcgttggtg gcaatacctt ctcccacgaa
    15301 tctggtattc accaagatgg agtccttaaa aatcctctca cttatgagat catcacacct
    15361 gaattggttg gtgttaagat tccgcttgga aaattatctg gtcgccatgc ttttgttgag
    15421 aaactgagag aattggccct agattttaca gaagaggata tcaaaccact ctttgctaag
    15481 ttcaaggcac tggccgataa gaagtaagaa atcacagatg cagatcctcg agctttggta
    15541 gctggaacca tggttgaaaa tccagaaggc ttccactttg atgatttaca acttcaaact
    15601 catgcagata atgacattga agcgctcgtt agcctagcca atatggatgg tgagaaagtc
    15661 gaatttaatg cgacagggca aggttccgtt gaagcaatct ttaacgctat cgataagttc
    15721 tttaaccaat ctgttcgttt ggtgtcctac actatcaatg cggtaacaga tggaatcgat
    15781 gcccaggatc gggttttggt cactgttgaa aacagagata cagaaaccat ctttaatgca
    15841 gcagggcttg attttgatgt gttgaaggct tctgctattg cctatataaa cgctaatacc
    15901 tttgttcaaa aagagaatgc aggtgagatg ggacgcagtg tttcttacca cgatatgcct
    15961 agtgtgtaaa ggagaaggct atggcaaaga aaatagtagc tctagtagga gacggaattg
    16021 gcccagaaat catggaggct ggtttagaag ttctggaggc tctagctgaa aaaacaggtt
    16081 ttgactatga gattgacaga cgaccgttcg gaggtgcaga tattgatgca gcaggacctc
    16141 ccttacctga tgaaaccctt aaggcaagta gggaagcaga tgctatccta ctagcagcta
    16201 tcggtagtcc tcagtatgat ggagcagcgg ttcgccctga acaaggcctg atggctctcc
    16261 gtaaggaact caatctttac gctaatattc gtcctgtaaa aatctttgac agtctcaagt
    16321 atttgtcacc actcaaaccg gaacgaattt ctggtgtaga ctttgtcgtg gtgcgtgaat
    16381 tgacaggcga gatttacttt ggagatcata ttcttgaaga gcgcaaagcg cgtgatatca
    16441 acgactatag ctatgaggaa gtggagcgga ttattcgcaa agcctttgaa attgcaagaa
    16501 atcgcagaaa aatcgttact agtatcgata agcaaaatgt tctagcgacc tcaaaactct
    16561 ggcggaaagt agctgaggaa gtcgcacagg atttcccaga tgtaaccttg gaacaccagc
    16621 tggtagactc agctgctatg cttatgatta ccaatcctgc taagtttgat gttattgtaa
    16681 cggagaatct ttttggagat attttatctg atgaatcaag cgtcttatct ggtacacttg
    16741 aggttatgcc atcagccagt cattctgaaa atggaccaag tctctatgaa cctattcacg
    16801 gttcagcacc tgatattgca ggtcaaggaa ttgccaatcc tacttccatg attttatcag
    16861 ttgccatgat gttgagagat agtttcggac gttatgagga tgcagagcgt atcaaacatg
    16921 ctgttgagac aagtctggcg gcaggaattt taacgagaga tataggaggt caggcttcaa
    16981 caaaggaaat gacggaagct attattgcaa ggttatgaag ttagacgaaa aaattactct
    17041 agtccttttg atttggaatg tcatcatttt cttgatttat ggcattgaca aattcaaggc
    17101 aagaagaaga acttggcgca tccaagagaa aatcttactc attttagccc ttacttgtgg
    17161 tggttttggg gcctggttag ctggaatcac ttttcaccac aagactcgaa aatggtactt
    17221 taaaacagtt tggtttcttg ggatggtgac cacactagta gccttatatt ttatttggag
    17281 gtaatggatg gcagggtctt cgagggaaca cgctgcttgg gttctagcgg actatggttt
    17341 taaggtcgtg attgcaggat ctttcggtga cattcattac aataatgaac tcaataatgg
    17401 cctgttgcca atcgttcagc ctagagaggt tagagagaaa ctagcccagc taaaaccaac
    17461 cgaccaggta actgtggact tggaacaaca aaaaatcatc tcaccagttg aagaattcac
    17521 cttcgagata gatagcgagt ggaaacataa actcctaaat agtttggatg atatcggtat
    17581 taccttgcag tatgaagagt tgattgctgc ttatgaaaaa caacgaccag cctactggca
    17641 ggattagaaa aaatagaaaa ggaaatatag taaactgaaa taagatgtaa acaaatgaat
    17701 tagagcttaa catccatttc cagcaatttt ttagaaacta cagtggacta ttctggattc
    17761 aacacattat aaaattatga caaaacacat tcacaagaag gctacgacat tttaaaaggt
    17821 gagggcggat gtatcgtttg ccctactaaa gttggttaca ttatcatgac cagcaacaag
    17881 gcaggacttg agcgtaagtt cgcagccaaa gaacgtaagc gtaacaaacc aggtgttgtt
    17941 ctctgcggta gcatggatga acttcgcgct ttagcgcaac tcaacccaga aattgaagca
    18001 ttctactaaa aacattggat gaagatattc ttcttggttg tatccttcct tggaaaccag
    18061 aagcctttga aaaactcaaa gcatacggga atggccgtga agaacttatg actgatgtac
    18121 gtggtactag ctgttttgtt atcaagtttg gaaaagcagg tgaacaattg gctgccaagc
    18181 tttgggaaga aggtaaaatg gtctacgcct catatgcttc aatgacaaaa cgattgaaac
    18241 tcgctatgag agcaaggtgt aatggtgtct atggtcgata aggacggcaa actcatccca
    18301 gaacaaggag gagcacgttc aacttcacca gcaccagttg tgatccgtaa agggcttgac
    18361 attgataaaa tcatgatgca cctgtcagat acttttaact catgggacta ccgtcaggtt
    18421 gagtattatt aagataaaaa aagaagtcta gtgttatgag atattaaagc tcctaacact
    18481 ggactttttg tttagaattt cttttctttt tctataggat atggtattct atgtagaaaa
    18541 tatatgttaa taagtaatgc caatatttaa acatcattag taaaaggagt tagattgatg
    18601 aataaaagaa aagttagttt agaagatttt tataaatggt atagtctaaa taaagaagag
    18661 ttattaaata aggcaactgt tggtgaaaag tttaatgata aattaaaaga agagtttctc
    18721 caggaatggc ctttggatag gattttaaca atgtcaatcg atgaatatgt aataggaaag
    18781 ggacagcaaa ataagtcttt atgctacgct cttgagaagg gaaaatacaa aaatctattt
    18841 cttggaattt ctggtggctc agcttcaaaa tttggtattt attggaataa aaaaacaaac
    18901 aaatataaag atcaagctaa taatgagatt tcagagttgg atcagcgatt ttcaaaatta
    18961 aaatcagatt tgtatgaaat tatcaaagaa ggtattcgtt ttaactttga aaatcctatt
    19021 tttgatatga aaagatcaac aaatgaattt attggtcgtt ctgctatggt gacaaaatta
    19081 ctttgtatct atactgaggg agatcctttc tttggtgtaa atattaatag tcagaaagaa
    19141 ttttggaacc actttgtttc tcagacaaat caaggtggac cttatctgca aaatcataaa
    19201 ataattgaac tagtgtccaa aacttatcct gagttggagc catcgaaatt aggaactatg
    19261 ctttttgagt attctaagct ttttatggaa aataaggaag acaatagtac aatggattca
    19321 tcaaacaatt ttcgtcatca attaactcaa tctctattaa agtctccaaa cctcatcctc
    19381 cgtggtgctc ctggcacagg aaagacttat cttgctaaag aaattgccaa agaattaacg
    19441 gatggcaacg aagaccaaat cggatttgta caatttcacc catcctatga ttatacggat
    19501 tttgtagaag gtttaagacc agtatcaaat ggggatggag ctattgagtt taggctacag
    19561 gacggtattt ttaaagattt ttgtcagaaa gcaaaagaaa cccaattgat tggaggacaa
    19621 gataattttg atgaggcttg ggattcttac ttagaatata taaatgttgc tgaagaaaaa
    19681 gaatatataa caaaaacatc ttacttatct attaatagta gacaaaattt gtcagtaaat
    19741 tatgatagtg gtgtttcagg atggtcacta cctagcaaat atgtttacga gttgtataaa
    19801 gataaaaatt ataataagca agaatactac aaaagtggtg gaaaaactgt cctagaaaca
    19861 ttgagaaaga gatttggttt gaaagactat gtttccccaa cagaaattga tactgataag
    19921 aaattcgtct tcatcatcga tgaaatcaat cgtggggaga tttctaagat ttttggcgaa
    19981 ctctttttct ctatcgaccc tggctatcgt ggtgaaaaag gaagtgtttc tacccaatat
    20041 gcaaatctac acgaaactga tgaaaagttc tatatccccg aaaatgttta catcatcgga
    20101 actatgaatg atattgatcg ttcagtggat acctttgatt ttgctatgcg tcgtcgtttt
    20161 cgttttgttg aagttactgc cgagggtcaa gttggcatgt tggataaaga gctgaatatc
    20221 catgcagaag aagcaaaaat tcgtctaaga aacttgaacg ctgctatcga aaatgttcag
    20281 gaattaaaca gtcattatca tattggacca agttattttc ttaagttgaa ggatgtagat
    20341 tttgactatg aattactctg gtctgattat attaagcctc tcctagaaga ctacttgcga
    20401 ggttcttatg atgaggttga aactttggaa actttgaaaa aagcatttga actgacaaat
    20461 aatgagcaaa aagatcaggc agtagctgat gacaatgaag gtgatgaaaa cgatgatgcg
    20521 gattactgat aatcaacaca agattattaa agaaaaattt gttgaagaat atcctaaact
    20581 aagcaatctt cttttagaca gaaccttgga aagtctatcc caagatgaac gtattttcat
    20641 ttttccaaat gatttgactc atactcctga tttggataag gaccaaaaga tttttgaaac
    20701 agtcaatcag aaaatcaaga cagggaacgt gattggtttt cttggatatg gtcaggaaag
    20761 attaacgatt tcctcacgat tttctgatga gagtaatgac cattttttgc attatctctt
    20821 aaacaaggtt ctttatatca atctcactag tttagatgtt gctttgtctc gtgaagagag
    20881 gctttatcaa cttttgatgt atctcttccc caagtatcta caagctgcta ttcgaaaagg
    20941 tctttataag gaatatcatc gattttttca taacgacagt aatgttaagg gagtgattga
    21001 tgtaagaagc catctcaaga aaaatcttcc tttcacggga aatattgcct acgcaacgag
    21061 agagttcacc tatgataatc ccctcatgca gttggtccgt cacactattg aatacattaa
    21121 gaatcagaaa agcattggtc aaggggtact agataatctc tcaactagtc gtgaaaacgt
    21181 atctgaaatc gtgcgtgtaa cgccctctta taaactagct gatcgtgcta agattactcg
    21241 gagaaatcaa tctaaaccta tacgtcatgc atactttcac gagtacagaa acttacaaga
    21301 actttgtctg atgatcctaa accaagaaaa gcacggttta gggtatcaag atcaaaaaat
    21361 ctatggtatt ctctttgatg ttgcctggct ttgggaagag tatgtttaca ccttgttgcc
    21421 aaaaggtttt gtacatccca gaaataagga taagacggat ggaatttcag tattttctgt
    21481 tgggaaacga aaagtatatc cagattttta tgacagagaa cgaaagattg ttctagatgc
    21541 aaaatataaa aaactggaat tgactgaaaa aggaatcaac cgtgaggact tattccagct
    21601 gatttcctat tcttatattt taaaagctga gaaggctgga ctgatttttc ctagtatgga
    21661 gcagtcagta aatagtgaaa taggaaaagt agctggctat ggagctcaat tgaagaagtg
    21721 gtctattcga atccctcaga atgcctcatc ctatagtgca ttttgtaaaa tgatggaaaa
    21781 ttcagaagaa aattttaaag cgattattga tgaagaagtg gggagaaaat aatttcccct
    21841 ctttctttta tttttaacac aagtaaaacc cgaacgatat attttaattc ataaaaaaaa
    21901 ttgacaaaaa ctgtactttg gtttataata aattaaggaa acgtcggaaa aggcgtagtg
    21961 atgcgagagc ataggtaggt cattacaaaa gaaacgagac atcgatatgt taaatgaatt
    22021 tccaattttt gattacgaag atattcaatt gattccaaat aaatgtgtca ttaaaagccg
    22081 tgcagaagcg gatacaagtg tcactttagg aaatcacacc tttaaactac ctgttgtgcc
    22141 agcgaatatg cagacgattt tggatgaaaa tgtagcagag caactggcta aaggtggtta
    22201 cttctacatt atgcaccgtt ttgatgaggc aggacgcatt ccttttatta aacgaatgca
    22261 caatcaaggg ctcattgctt ccatatctgt cggtgttaag gactatgagt atgatttcgt
    22321 tagacagctc aagactgatg ctcctgagta tatcactatt gatattgctc atggtcatgc
    22381 ggatagcgtg atttctatga ttcaacacat caagaaagaa ttgccagata cctttgtcat
    22441 tgctggaaat gtgggaacac cagaagctgt gcgtgaattg gaaaatgctg gtgcggatgc
    22501 tactaaggtc ggaatcggtc ctggtaaggt ttgtatcacc aaggttaaga ctggttttgg
    22561 tacaggtggt tggcagttgg ctgctctacg ctggtgtgtc aaggctgcgc gtaaaccgat
    22621 tatcgctgat ggaggaattc gtactcacgg tgatattgcc aagtctatcc gcttcggtgc
    22681 tagcatgatc atgattggtt ccctctttgc aggacatatt gaaagtccag ggaaaacgat
    22741 tgaagtcgat ggtgaacagt tcaaagaata ttatggttca gcctcacaat atcaaaaagg
    22801 tgcttacaaa aatgtggaag gcaaacgtat cttgcttcct gctaaaggtc atctgcaaga
    22861 cactttaact gagatggaac aagaccttca aagtgctatt tcttatgcag gtggacgtca
    22921 ggttgctgac cttaaacacg ttgattatgt gatcgtgaaa aactccatct ggaatgggga
    22981 tgcttcccac taagacgggg tctttgacta aaaaacttgt tattagagca aatttctgtt
    23041 ataataaaac aagtttccac ccttagtgta atggatatca cgtaagattc cggttcttga
    23101 gatgggggtt cgattccctc agggtggatg taaacattct aaaaagcctt taatagggct
    23161 tttttcttta cactgctcta aatttaccgc taaaactgag aatctctgtc taaagaattt
    23221 ccaaatgctt tccagtcccg ttttagagtg actcaggggg ctttttttga tataataaaa
    23281 aggactgtta tcagttagaa agaggttggt atgaaagaat tacaaactgt actaaagaac
    23341 cattttgcaa tcgaatttac agacaaaaag ttactggaaa ctgcctttac tcatacgagt
    23401 tatgccaatg agcaccgcct cttaaaaatt tcacataatg aacgcttgga atttttagga
    23461 gacgctgttc tacagttatt gatttcagaa tatctgtata aaaaatatcc taaaaagcct
    23521 gaaggtgacc tatcaaaact ccgtgctatg attgtccgtg aggagagttt agctggtttt
    23581 gcgcgtgatt gccagtttga ccagtttatc aagttgggta aaggggaaga aaaatctggt
    23641 ggtcgcaatc gtgacaccat tcttggtgat gcctttgaag cctttcttgg tgcccttctt
    23701 ttggataagg atgtggccaa ggtcaaggaa tttatctatc aagtcatgat tcctaaggtt
    23761 gaagcaggcg gatttgagat gattacagac tataaaaccc atctccaaga gttacttcag
    23821 gtcaatggtg atgtggctat tcgttatcag gtgatttctg aaacagggcc tgctcacgat
    23881 aaggtctttg atgtagaagt tcttgttgaa ggtaagagta tcggtcaagg ccaaggtcgt
    23941 tctaagaaat tagcagagca ggaagctgcc aaaaatgccg ttgagaaggg gctggattca
    24001 tgtatttaaa ggaaatcgaa attcaggggt tcaagtcttt tgctgataag accaaggtcg
    24061 tttttgacca aggtgtgacg gcagttgttg gacccaatgg atctggaaag tccaatatta
    24121 cagaaagtct gcgttgggct ttgggggagt ctagtgtcaa gagtctccgt gggggcaaga
    24181 tgccggatgt catctttgct ggaacagaaa gtcgcaaacc gctcaattat gcttctgtag
    24241 ttgtgactct ggataatcat gacggattta tcaaggatgc aggtcaagaa atcagggtgg
    24301 aacgccatat ctatcgtagt ggagatagcg aatacaagat tgacggcaag aaagtccgtc
    24361 tgcgtgatat tcatgacctc ttcttggata ctggattggg acgagattcc ttctctatta
    24421 tttcccaagg gaaggttgag gagattttta attccaagcc tgaggaacga cgagctattt
    24481 ttgaagaagc tgctggagtt ttaaaataca agactcgcag aaaagaaacc gagagtaaac
    24541 tgcagcaaac tcaggataat ctggaccgct tagaggacat tatctacgag ttggataatc
    24601 aaatcaagcc tcttgagaag caagctgaga atgcccgtaa gtttttagac ttggaaggac
    24661 aacgtaaggc tatttattta gacgttctgg ttgctcaaat caaggaaaat aaggctgaac
    24721 tagagtcgac agaagaagag ttggctcagg ttcaagaact cttgatgagt tattaccaaa
    24781 agcgtgaaaa attagaagaa gaaaatcaaa ctcttaaaaa gcaacgccaa gatttacagg
    24841 ctgaaatggc caaagaccaa ggcagtttga tggatttgac cagtctgatt agtgacttag
    24901 agagaaaatt agccctatcg aaactggaat ctgagcaagt agccctcaat caacaggaag
    24961 cacaagcccg tttggctact ttggaggata agagaagttc actcagccaa gaaaaatctg
    25021 ataaagaaag ctctttagcc ctcttggaag aaaatctagt ccaaaataat caaaaactca
    25081 atcgattaga ggctgaattg ctagcttttt cagatgatcc tgatcagatg attgagctct
    25141 tacgtgaacg ctttgtggcc cttttacaag aagaagcaga tgtctcaaac caactgaccc
    25201 gtattgagaa tgagttggaa aatagtcatc agctttctca aaaacaagca gatcaactag
    25261 aaaagctgaa agaacagttg gctacagcta aagagaaggc tggtcagcaa aaggctgagc
    25321 ttgaaactgc caaggagcag gttcagaaat tattggccga ctatcaagtc tgtgctaagg
    25381 agcaagagga gcagaaaact tcctatcaag ctcaacaaag tcaactcttt gaccgtctgg
    25441 atagtctcaa aaacaagcag gctagagcgc aaagtttgga aaatatcctg agaaatcata
    25501 gtaactttta tgcaggtgtt aagagtgttc tccaagagaa agatcgtcta ggtgggatta
    25561 ttggagcagt tagtgagcac ctgacctttg atatgcatta tcaaactgcc ctagaaattg
    25621 cgcttggagc gagtagtcag cacattattg tagaagatga gaatgcggca accaaagcca
    25681 ttgatttact caaacgaaac agatctggtc gtgcaacctt ccttcctttg accactatca
    25741 aggctcgtac gatttctagt cagaaccaag atactattgc tgcaagtcca ggatttctgg
    25801 gcatggcaga tgagctggtt actttcgata ctagactgga agccattttc aagaatttgc
    25861 tagctacaac ggccattttt gataccgtgg agcatgcgcg tgcagcagcg cgccaggttc
    25921 gttatcaggt tcgtatggtg acattggacg gaacagagct gcgcacaggt ggttcttatg
    25981 caggtggagc taatcgccag aataacagta ttttcatcaa gccagaactg gagcaattac
    26041 aaaaagaaat tgctgaagaa gaagcaagtt tgcgttcaga tgaagtgagt ttgaagaact
    26101 tgcaagatga gctagctaga ttgacagaaa gattagaagc catcaaatct cagggagagc
    26161 aggctcgtat tcaggagcaa ggcttgtccc tcgcctatca gcagaccagt cagcaagttg
    26221 aagagctaga aactctttgg aaactccaag aagaggaatt aaatcgtctt tctgagggag
    26281 aatggcaagc ggataaggaa aaatgccaag agcgccttac tacaatcact agtgaaaaga
    26341 ataatctgga agctgagatt gaagagatta agtctaataa aaacgccatc caagaacgtt
    26401 atcaaaactt gcaggaagag ctagctcaag ctcgtctact taagacagaa ctgcaagggc
    26461 aaaaacgtta tgaagttgct gatattgaac gcttaggtaa ggaactagat aatcttgata
    26521 ttgagcaaga agaaattcag cgccttcttc aagaaaaggt tgacaatctt gagaaagttg
    26581 atacggattt gctcagtcaa caggctgatg aagccaaaac tcaaaaaaca aatctccaac
    26641 aaggtttgat tcgcaagcag tttgagttgg atgatattga gggtcaactg gatgatattg
    26701 ctagtcattt ggatcaggct cgccagcaga atgaagagtg gattcgcaag caaactcgtg
    26761 ctgaagccaa gaaagaaaag gttagcgagc gcttgcgcca tctacaaaat caattaacag
    26821 accagtacca gattagctat actgaagcac tagaaaaagc gcatgagttg gaaaatctca
    26881 atctggcaga gcaagaggtg caggatttag agaaggctat tcgctcactg ggacctgtca
    26941 atttagacgc tattgaacag tacgaagaag ttcacaaccg tctggatttc ctaaatagcc
    27001 aacgtgatga tattttgtct gcgaaaaacc tgcttcttga aaccatcaca gagatgaatg
    27061 atgaggtcaa ggaacgcttt aaatcaacct ttgaggccat tcgtgagtcc tttaaagtga
    27121 ccttcaagca gatgtttggt ggaggtcagg cagacttgat attgactgag ggcgaccttt
    27181 taacagctgg tgtggagatt tctgttcaac ctccaggtaa gaaaatccag tcgcttaacc
    27241 tcatgagtgg tggtgaaaaa gctctatcgg ctcttgcctt gcttttctcc attattcgtg
    27301 tcaagaccat tccttttgtc atcttggatg aggtagaggc tgcgctagac gaagccaatg
    27361 ttaaacgttt cggggattat ctcaaccgct ttgataagga cagtcaattt attgtcgtaa
    27421 cccaccgtaa ggggaccatg gcagcggccg attctatcta tggagtgacc atgcaagaat
    27481 cgggtgtttc aaagattgtt tcagttaagt taaaagattt agaaagtatt gaaggatgac
    27541 aattaaacta gtagcaacgg atatggacgg aaccttccta gatgggaatg gacgctttga
    27601 tatggatcgt ctcaagtctc tcttggcttc ctacaaggaa aaagggattt actttgcggt
    27661 ggcttcgggt cggggatttc tgtctctgga aaaattattt gctggtgttc gtgatgacat
    27721 tattttcatc gcggaaaatg gcagtttggt agagtatcaa ggtcaggact tgtatgaagc
    27781 gactatgtct cgtgactttt atctggcaac ttttgaaaag ctgaaaacgt caccttatgt
    27841 agatatcaat aaactgctct tgacgggtaa gaagggttca tatgttctag atacggttga
    27901 tgagacctat ttgaaagtga gtcagcatta taatgaaaat atccaaaaag tagcgagttt
    27961 ggaagatatc acagatgaca ttttcaaatt tacaaccaac ttcacagaag aaacgctgga
    28021 agctggtgaa gcttgggtca atgataatgt ccctggtgtc aaggctatga caactggctt
    28081 tgaatctatt gatattgttc tggactatgt cgataagggt gtagctattg ttgaattagc
    28141 taaaaaactt ggtatcacaa tggatcaggt catggctttt ggagacaatc ttaatgactt
    28201 acatatgatg caggttgtgg gacatcctgt agctcctgaa aatgcacgac ctgaaatttt
    28261 agaattagca aagactgtga ttggtcacca taaggaacgg tcggttatag cttatatgga
    28321 gggcttataa tggcagatat aaaattgatt gcattggact tggacgggac cttgctgact
    28381 actgataaaa ggctgacgga tcgtaccaag gaaaccttga aagctgcgcg tgatcgtggt
    28441 atcaaggtcg tattgacaac tggtcgtccc ttaaaagcca tggatttctt tctccatgag
    28501 ttagggactg acggtcagga agatgagtat accattactt ttaatggtgg attagttcag
    28561 aaaaatacag gagaaatcct tgataaaaca gtcttttcat atgatgatgt ggcacgtttg
    28621 tatgaagaaa cagagaaatt atcactgcct ctcgatgcca tctcagaagg aacagtttat
    28681 caaatccaat cggaccaaga aagtctttat gccaaattca atccagcttt gacctttgtt
    28741 ccagtggact ttgaagactt atctagtcaa atgacctaca acaaatgcgt gactgccttt
    28801 gctcaagaac ccttggatgc agccattcag aagatttctc cagaattgtt tgaccaatat
    28861 gaaatcttta aatcacgtga aatgttgcta gaatggtcac caaagaatgt tcataaagca
    28921 acaggtttgg caaaactaat cagccatctt ggaatcgacc aaagtcaagt gatggcttgt
    28981 ggtgacgagg ccaatgacct ctctatgatt gaatgggcag gtcttggtgt tgctatgcaa
    29041 aacgctgttc ctgaagtaaa ggcagccgca aatgtagtga cgccgatgac caacgatgag
    29101 gaagctgtcg cctgggctat cgaagaatat gtgctaaagg agaactaaga tatgggattg
    29161 tttgaccgtc tattcggaaa aaaagaagaa cctaaaatcg aagaagttgt aaaagaagct
    29221 ctggaaaatc ttgatttgtc tgaagatgtt gatcctacct tcacagaagt tgaggaagtt
    29281 tctcaggaag aagcagaggt tgaaattgtt gaacaagctg tgttccaaga agaggaaatc
    29341 caagacacag ttgaagaaag tctggattta gagccagtta tagaagtttc tcaaaaagaa
    29401 gtcaaagaat ttccacactc agaagaaggg aatactgagt ttctagagac tgtagaagaa
    29461 aataattctg aaattcttga agcagaaagg cctcaaacag aagaaactgt tcaggaaaaa
    29521 tatgaccgca gtcttaagaa aactcgcaca ggtttcggtg cccgcttgaa tgccttcttt
    29581 gctaacttcc gctctgttga cgaagaattt ttcgaggaac tggaagaact gttgattatg
    29641 agtgatgttg gtgtccaagt cgcttctaac ttaacggagg aactacgtta cgaagccaag
    29701 cttgaaaatg ccaagaaacc tgatgcactt cgtcgtgtca tcattgagaa attggttgag
    29761 ctttatgaaa aggatggtag ctacgatgaa agcatccact tccaagataa cttgacagtt
    29821 atgctctttg ttggtgtgaa tggtgttggg aaaacaactt ctatcggaaa actagctcac
    29881 cgctacaaac gagctggtaa gaaggtcatg ctggttgcag cagatacctt ccgtgcgggt
    29941 gcagtagctc agctagctga atggggccga cgagtagatg ttccagtagt aactggacct
    30001 gaaaaagctg atccagccag cgtggtcttt gatggtatgg aacgtgccgt ggctgaaggt
    30061 atcgatattc tcatgattga tactgctggt cgtctgcaaa ataaggataa ccttatggct
    30121 gagttggaaa agattggtcg tattatcaaa cgtgttgtgc cagaagcacc acatgaaacc
    30181 ttcttggcac ttgatgcatc aacaggtcaa aatgccctag tacaggccaa agaattttcg
    30241 aaaatcacac ctttaacggg aattgttttg actaagattg atggaactgc tcgaggaggt
    30301 gtggttctag ccattcgtga agaactcaat attcctgtaa aattgattgg ttttggtgaa
    30361 aaaatcgatg atattggaga gtttaactca gaaaacttca tgaaaggtct cttggaaggt
    30421 ttaatctaat cagaagcaaa aatcctgcaa ggcataaact tgcaggaaat ttttttattc
    30481 taagcgacca tcttgacgat aggcgatatc tggttgccaa gtccatttgg caccgaattt
    30541 ttcaagtagg tcaaagctgg cttgaggtcc catgcttcca gctttatagt catgaagtgg
    30601 ggcaccattt tcatcccaga gctcttcaat acggtcaatc aacttccatg atgcaccaac
    30661 ttcatcccag tggctaaagt tagttgagtt gttatttagg acatcataaa tcaatttctc
    30721 gtatggttct ggagaagcac cagttgcagt cgcatctgta cggtaatcaa gtgagttagg
    30781 agccaagtta aattcttctc ctacttgctt cccatttagg ctaagagaga agccttctgt
    30841 tggttgaata tagatggtca aaatatttgg agcaagtggt tctccaaaga tagaatccat
    30901 ttgtttaaag acgatgttga catgagttcc tttttcagtc agtcgtttac ctgtacggaa
    30961 aaagaaagga acactacgga atcgatcgct gtctacaaag aaggcaccag atgtaaaggt
    31021 ttcagttgtt gattctggat tcacatttgg ctcgctacga taagagatgt atttcatgcc
    31081 atcaatctta ccagagcggt attgcccacg gataaagtgt tctttgagtt cttcatcagt
    31141 tggatgatag aggtttttaa agaccttaat cttttcagca cgaatctcgt cttttgtgaa
    31201 gcttgctggt ttgtccatgg cgaggagcga aagaagttgt agagtgtggt tttggaccat
    31261 gtcacggagg gcaccggatt ggtcatagta gccaccacgt tcttctacac ccaagcgctc
    31321 cgcaaaggta atttgaacat tgtcgataaa atccttgttc caaacgtttt caaaaatcaa
    31381 gtttgcaaag cgaactgcaa agatgctttg gatcatttcc ttaccaagat aatggtcgat
    31441 acggaaaatt tgttcttcgt caaatgttgc taggagttcg tcattcaact tgcttgcagt
    31501 tgcgtaatct gtaccaaatg gtttttcaac gatcaagcgc tcaaaacctt tgccatcgac
    31561 aatgttttca gatttgaggt gtttggcaat ggttccaaag aactgaggtg ccatagacaa
    31621 gaagaagagc ttattgtgtt cagcttggta tttttcatta agctcagctt gtaattgacg
    31681 caaagcaata taatgttccg aatcattgac atcatggctt tgatagtaga agtggctagc
    31741 aaattcttgg gcttgctcgg tactatctgc caaatcaagg atggactcga caactacaga
    31801 ttcaaaatat tccttactcc aaggtctacg ggcagttcca ataacggcaa agtgcttgga
    31861 aagattgccg gatttatata gtctaaaaag ggaagggtag agcttgcgtt tagccaggtc
    31921 tccactcgca ccgaaaattg taacaataac cttagatgac atctagctac ctaatttcta
    31981 tttttattcc tagtcttgct aggtatgagg aaacctcatt tcataaactt aattctaaca
    32041 taattttccg aaaaatcaaa aattccaata cgaagtagaa aaactgcaag gaaatcctta
    32101 cagtttgagt ttagacgttt aagaccttat ctaagaactc tttcagacga gggtgttgtg
    32161 ggttatcaaa gatttggtca ggtgttccgt cttcaaggaa ctcgccatct gcagtaaaga
    32221 taacgcggtt ggcaacctga cgagcaaatc ccatctcatg ggttacgata atcatggtca
    32281 taccttgctc agccaattcc ttcataacgt taagtacgtc tccaaccatc tcagggtcaa
    32341 gggcagaagt tggttcatcg aagagcatga tgtctggatt cattgctagg ccacgagcga
    32401 tggccacacg ttgtttttga ccacctgata ggctatctgg attggcatta gctttatctg
    32461 ctagtccaac cttttcaagc aactccattc ccaattcctc agcttcttcc ttagtcatca
    32521 acttgtgctc aataggagca aaggtgatgt tgtccaatac agacatatga gggaagaggt
    32581 tgaagtgttg gaataccatg ccgatatttt cacggacgtg gtcaacattg gttgtttttt
    32641 cagttaaatc atagccgttc acagtgatgt gaccgctagt gacttcttct aaaagattga
    32701 ggctacggag gaaagttgac ttaccagaac ctgaaggacc gatgatacaa acaacatctc
    32761 cttcatagaa cttagtcgta attcctttta ggacttcatt ttttccatag tgcttgtgta
    32821 aatcatttac atcaattttt aattttgcca ttaacgaatc ctcttttcta agcgtttcgc
    32881 tagtctagtc aaaagtgtga taattacaag atagaagata gcaaggattg catacatctt
    32941 gaaactttgg tagttacgag caatgataat cttaccagtt tggaagagtt caaccaaacc
    33001 gatagcagat acgatagttg tatctttaag agcgataacg aattggttga caaagtttgg
    33061 caacatcaat ttagttgctt gtggcaagat aatcttacgc atggtttttc cataagagat
    33121 acccaagctt cggctggctt ccatttggcc aactggaacg gcctgaatac caccacgaac
    33181 gatttcagcg atataagcag ccgcattgag tgagagggca atggttccag ctacaaagtc
    33241 gttaattggg ctttgttggc ctgtgataga ctcgatgaag tttggaattc cccagaagat
    33301 gaaggctgca agaatcatca atggaatacc acgaataacg tcaacgaaaa tctcagagat
    33361 gacgcgaaga gatttgtatg ggctaacgct aaacataccg aagataattc cgatgacaat
    33421 ggcaatagca aatgagataa gagctagagc aagagtgata ccaagaccgc taaggagttg
    33481 tttgtagttg ttttgaagca agccccagag cgttgtttcg tcaacagtac ttgttgaagc
    33541 agttgaagat tcgctagcta ggtatttgtc aagaatcttt tggaattcac cgtttgcttt
    33601 aaggtttgca agtccgttgt tgaacatttc aatcagttct ggatttgctc cttttttaac
    33661 ggcaaaggct gtttcaccga ttggagttcc agagattgga gttttcaatt tttgaccttg
    33721 gctgatagaa tatttgagaa caggttcatc atccataacg gcatcaatgg caccagtgtt
    33781 taaactgtca tacattgaag aaccatcagc aaaggttttg attttgtaac cgtatttgct
    33841 ttgattttct gttaggaagg tttgagaagc agttccgttt ttaacaccga ctgtctttcc
    33901 ttttagatct tcataagaag caatattgct tgattctttg acaccaagaa tggtattagc
    33961 agtgtagtat gattctgaga agtcaaaagt tgccttacga gcatctgtga cagacatccc
    34021 agcaatgata ccatcggctt gaccagcttg gacagcactg atagcagcat caaaaccagg
    34081 gttggtgatt tcaatttcaa aaccttggtc tttagcgatt gccttaatca attccatatc
    34141 aataccagtg tattggttgc ttgaattttg gaaaacaaaa ggtgcaaaag aagaatcgct
    34201 ggcaatgata tatttagcct taacaggaat agcttttaat cctgcgagag tagttgtagt
    34261 tggcactgct gaagatgatg aagcagtcca tttcttgata attttatcaa gactaccatc
    34321 ttttttcatt tcagccaagg cttggttaaa ttcagtaacc aggtgctcgt atttacttcc
    34381 ttttttcaca ccgaaagcaa aacttcctac agcttcacca tccatttcaa tatggagatc
    34441 ttgaccttgg ttaatggcat attcgataac aggtttgtca tccatcatgg catcgatggc
    34501 accagcactc aagctgttgt tcattaaatc accagtgtca aatgttttaa tagtaaagcc
    34561 gtatttatct ttgattgttt caaggaaacg ttgagcggca gttccgtttt taacaccaac
    34621 ggttttgcca gttaattggt cgtacttgct aattttgtgt gactttgtag tagcaatgac
    34681 aacttttgta tcatagtaag tatcagacat ggtgaagact ttttcacgtt ctttagtctt
    34741 tgtcatccct gccatgatag cgtcggcttg cccagcttga accgcattga ctgctgcgtc
    34801 aaatccagga taggacatct gaatgttcca gcctttaatc tcagcgactt tgttaataat
    34861 gtcaacatca attcctttat aagtttgatc tgaatcttta aactcaaaag gtgcataggc
    34921 ggtatcagaa acaatcttaa tcgtttctgc tttggcaata cctaatgaga aaattgggaa
    34981 taaaattagc aaaaatgcta gaaatttttt cttcattttt agtctccttt tccgaatatt
    35041 ctctcattat atcagaaaaa gtaaaaaaag gcaaattgtt atatttttcg tgtcagaaaa
    35101 tataacattg atttttcctt ttctttcttg aattgctatt aaaaagtgct ataataatag
    35161 tatatataga agaaaagagg acggggatat gaagaacaaa agaatattta aagacttcca
    35221 agcttcaaaa atgagtttaa acatttacac aagccccttg ttagcctttg tttttgtctt
    35281 cataggagag tttgtggctt ttactttgta tggtattggc ttgttagctc tcatcggact
    35341 tgctagaaat tttggagagg ctggtcaaaa tcttgcaagc tacttgcaga ccttgcaaca
    35401 gagcttgacg gataaaacaa gtgactttcg tttaatttta ggattactgg cctttggttt
    35461 tattcttaac actgtgttca gatggacaag aaaagttgag aaaagaccta ttcgaacctt
    35521 gggattttat agagagaatt tcctcagcaa tcttctgaaa ggatttagtc taggcttggc
    35581 actttttctt ctgaccttgt taggtttagt ggtcttaggt caatatcgtt tggaatccat
    35641 tcacttgaat ccttattctc ttgcctttgt cgtctttact atcccatttt ggattttaca
    35701 ggggacagca gaagaagtgg tggcccgtgc ttggctactt cctcaattgg cctcaagaac
    35761 caatctaaaa ctagctattc ttatatctag cctgttcttt accctgcttc atatgggcaa
    35821 ttctagcctc acccctctat ctctagtaaa tctcttttta ttcggagttg ccatggctct
    35881 ttatcttctc aaaactgata cagtttgggg tgttgcaggt attcatggtg cttggaattt
    35941 tgctcagggt aatctctttg ggattttagt tagtggtcaa ccgtcaggaa cgtctctgat
    36001 gaccttttta ccacaaggca atcaagattg gctatcaggt ggttcttttg gcatagaagg
    36061 ttccattatg acaagtctgg tattactact gctgattgtc tatcttgcta ataaattaaa
    36121 gaaagaaaat gaaaggatgt gacttcggtc cgtccttttc ttcgtgaaaa tactataagt
    36181 atgctaaaat aggaatagca catggagaga ggattcttat gatcaatcac attacagata
    36241 atcaatttaa actagtatca aaatatcaac catcaggaga tcaaccccaa gctatcgagc
    36301 agttggtgga taacattgag gggggagaaa aagctcagat tctgatgggg gcgactggaa
    36361 cagggaagac ctatactatg agtcaggtca tttctaaagt caataaacca actctggtta
    36421 ttgcccacaa taaaactctg gctggtcagc tctatgggga gtttaaggaa tttttccctg
    36481 aaaatgcagt tgagtatttc gtatcctact atgattatta ccagccagag gcctatgtcc
    36541 cttctagcga tacctatatt gagaaggata gttctgtcaa tgacgagatt gacaagcttc
    36601 gccactcagc tacctcagcc cttttggagc gtaatgatgt tattgtcgtg gcctcagtct
    36661 cttgtatcta tggtttgggt tcgcccaagg aatacgctga tagtgtcgtt agtctccgtc
    36721 ctggtctaga gatttctcgt gataaactct tgaatgactt ggtcgatatt cagtttgaac
    36781 gtaatgatat tgatttccaa cgcggaagat ttcgtgttcg tggggatgtg gtagagattt
    36841 tcccagcttc ccgagatgaa catgcctttc gagtagaatt ttttggagac gaaattgacc
    36901 gtattcgtga agttgaggct ctgacaggtc aggtgttggg agaagtggat catttagcga
    36961 ttttcccagc gacacacttt gtgaccaatg acgaccacat ggaagttgcc attgcaaaga
    37021 ttcaggccga gttggaggag cagttagctg tctttgaaaa ggaaggtaaa ctgcttgaag
    37081 cccagcgttt gaaacagcgg acagagtatg atatcgaaat gttgcgtgag atgggctata
    37141 ccaatggggt tgaaaattat tctcgccaca tggatggacg gagcgaagga gagcctcctt
    37201 atacgcttct cgacttcttc ccagatgatt tcttgattat gattgacgag agtcatatga
    37261 ccatagggca aatcaagggc atgtacaatg gagaccgttc gcgtaaagaa atgctggtta
    37321 attatggttt ccgtttgccg tctgctttgg acaatcgtcc tctccgtcgg gaggagtttg
    37381 agagtcacgt tcatcagatt gtttacgttt cagcgacacc tggtgactat gaaaatgaac
    37441 agaccgagac agtgattgag caaatcattc gtccaacggg actcttggat ccagaggtgg
    37501 aagtccgtcc gactatggga cagattgatg acctcttggg tgaaatcaat gcccgcgttg
    37561 aaaaaaatga gcgtaccttt atcacaactt tgaccaagaa aatggcagag gacttgaccg
    37621 actacttcaa ggaaatgggt atcaaggtca agtacatgca ctcggatatc aagaccttgg
    37681 aacggacgga gattatccgt gacctgcgct tgggtgtctt tgatgtcttg gtcggaatta
    37741 acctgctccg tgaaggaatt gacgttcctg aagtgagcct cgtagctatt ctcgatgctg
    37801 acaaggaagg tttccttcgc aacgaacgtg gactcatcca gactattgga cgtgctgctc
    37861 gtaacagtga aggccatgtc atcatgtatg cagacacggt tacccagtct atgcaacgtg
    37921 ctatcgatga aactgcccgc cgtcggaaaa ttcagatggc ctataatgaa gagcatggta
    37981 tcgtaccaca gacaatcaag aaagaaatcc gtgacctgat tgccgtgacc aaggcagttg
    38041 ctaaggaaga ggacaaggaa gtcgacatca atagcctcaa caaacaagag cgtaaagaac
    38101 tcgtcaagaa actagaaaaa caaatgcaag aagccgtcga agtgcttgac tttgaactgg
    38161 cggctcagat ccgtgatatg atgttggaag tcaaggcctt ggattagggg aatagtatga
    38221 tttatttaag aaagttaaag agagaagatt tgatgtcttt atgggaaatg gcttattcac
    38281 aacttaatcc agtttggaaa cagtatgatg ctccctatta tgatgattat cagtattttt
    38341 caaattttaa agaattcgaa ctacaaaaat cagaatccat tttaagcaac tcaaatcgcc
    38401 ttggtatttt tgttgatgat aaactagttg ggactgtttc gcgttattgg gtatgtaaac
    38461 aaacaagatg gatggaattg ggaattggta tttatgataa aaaattctgg aacactggta
    38521 ttgggaaagt tgctatgttg cagtggatag ataggacgtt tcaggattac ttggagttgg
    38581 agcatctggg tttgacaact tggtcaggga atcttggtat gatgaaactt gctgaaaaat
    38641 taagaatgaa aaaagaagct catattccaa aagttcgtta ttatcaaggt aaatattttg
    38701 atagtattaa atatggtatt ttgagagaag actgggagaa aataaatgac ggttattatc
    38761 aaatcaatgg aaactcctaa agagatagaa ggtaaatccc ttgttcactg gcaaatgtgg
    38821 agagaggctt atgatgacct tttgcctgca gaatttcagg agacaatgac attagaaaga
    38881 tgtcgactct ttagtcaaaa gtatccagaa aatacattga ttgcgatagg tgatctgaag
    38941 gtagttggtt ttatcagtta tggtaacttt cgtgatgaga ctatgcaagc tggtgaaatt
    39001 attgctttat atgttttaaa agactattat ggaaaaggta tcgcacaaaa gttaatgaaa
    39061 gcagctttga ctgctcttaa tcatttttct gaaattttct tatgggtatt gaaagataac
    39121 aagcgcgcca ttgctttcta tcaaaaaatg ggttttactt ttgatggaca agaaaaaata
    39181 cttgaacttg gaaagcctat aaaggaaaaa cgaatggtgt tctattctaa ataattttaa
    39241 aaaagtaaaa gataatatct ctggtagtaa gtctgaaaat ttaataaatt agaaagcgag
    39301 taaatttatg tcccgttccc aattaacaat tttaacaaat atctgtctga ttgaagacct
    39361 cgaaactcag cgcgtggtga tgcagtatcg cgcccctgaa aacaatcgct ggtctggtta
    39421 tgcctttcct ggaggtcatg tagaaaatga tgaggctttt gcggagtctg tcattcgtga
    39481 aatctacgaa gaaacagggt tgactatcca aaatcctcaa cttgtcggca ttaaaaattg
    39541 gccactagat acaggtgggc gctatattgt catttgttat aaggcgactg agttctctgg
    39601 tagccttcgg tcttcagatg aaggagaagt ttcttgggtg caaaaagacc agattccaaa
    39661 cttggatctg gcctatgata tgctaccatt gatggaaatg atggaagctc ccgacaagtc
    39721 agagtttttc taccctcgcc gtacagaaga cgattgggaa aagaaaatct tctagtcttt
    39781 tactaaataa cctagctgat ccaaggcctc ctcgatatag tggaggtctt gttgtgtttc
    39841 ggcttcgact aggtggtaat ggatgccatc tgtcaattca gatagaggtc taaagtcaga
    39901 atgctcgact tgttctagaa aatgttgcac atctctgcga caagacagtt taagcaaggt
    39961 ttcaatttct ccataaacgg gatggtcaat caaggtattc tggacatgcc caccattatc
    40021 gacaatagca aggagttcct gaccgatttc ttcaacttca tgtttcactt tgaaaagttt
    40081 gtgaacataa ggattggtat caatttgctt atagatgtag ccacgattgg tggataggat
    40141 aggagcgcca tcggctctca aaattgcaat gtcctgcaca atgacctgac gtgtgacatg
    40201 aaaatgttcc gccaaacttt ggccattaag ggctttagga gcttctttca agagttggag
    40261 cagggcttgt ttgcgatctt ttgtcatagc ttttcctttt aacggcgttt tcgaagcact
    40321 ttatagacag ctagtgctaa tgtatagtct accatactat ggataattgt gccaaatcca
    40381 actagtacaa atagaacata gaacatattt tctacattgg tacccgaagt tgcgtaaaaa
    40441 aggacacagg ctaatacttc agcaagggca tgaacaacag ccaaaacaaa attgaaaacc
    40501 caggaagatt ttggtttatc tagggtatcg gggaattttt gtaggtaaag agctcctaaa
    40561 gcaccaaaag atatatggga aaaagcccga aaaacgataa ccatgggata gccagccatc
    40621 aaaaatccaa aactagaggc taggatgaca aaaactgcca tcaagggcga caagaacatg
    40681 gctataaaaa tagcgatgtg gctccccaaa gtataggaag caggtggaat gacaatcttg
    40741 aaaggcataa caattggaat caaaatcgca atagccgtta aaagggctgt cattgtcata
    40801 aattgtgtct ttttccgtgt attcacaaga atctcctttt taactgcata tacactagta
    40861 tggtacaata aaccagacaa taaagcaaga atttacttgg gtttatagat cattttttag
    40921 ttaaaagtta tagtagatgg aaactagaat agtccacctc tacttctaaa acattgttag
    40981 cgatttgact gtcctgatcg atttgtcctg ttcttatttc gttttactat agtaaagatt
    41041 tcattaaaaa gaaactgtat agagcaaaat ctccaccttc aggtttgaaa gcggagattg
    41101 tttttatttt ttcaaggttt gtagtcgtgg aacaatggct agggttagaa ttgcagaaat
    41161 gaccaattca gcaattgaat ttgttgagat aacggttgcc agaagaagtt ggatatttcc
    41221 attataaaca tttccaaata ggaagaagat tcctccaagg acaaagatag tatttgtcaa
    41281 ggaaccaagg gctccagcta aaatcagacc agtcttgttt ttcatcagtt tatagactaa
    41341 gtaaggagtt aaaccaatca aaatacgtgg gacgatggca atgatagctg agtagatgtt
    41401 tccgtttggt acgaagggag agaagaggta gcttgtcggt agaatcgtaa tcgtgttaac
    41461 cgtcaagcta agtaatccca tcaaaaatcc aagtgtaacc ccaactcgtg gaccataaat
    41521 aatgctggca ataatgacag gaatatgaac aatggtcggt ttgattggaa atggaaaaag
    41581 gttaaagata agtgagctca gaaagtgtat cacgagcatg gtagcaaaaa agatagcaat
    41641 gggtgcaata ttagagcgtt ttttcatcga tagtttcctt tattctttct aaaataattg
    41701 tgaggtcagc taaagctcct cgtccgtggt ctccacaagc tagtagggat tccttaggag
    41761 caatcagctg atagccgtag gtttctaatg ttttcagatt attctgagtt actggatggt
    41821 catacatttt tgtattcata gcaggagcta ttagtttggg aatatgactt ggtagggcta
    41881 gagctgtact ggttaccatg ttgtccgcaa atccgtgagc tagttttgca atagtgttag
    41941 cagttgcagg taccacgata aataaatctg ctttttttcc aagttcgata tgattgacct
    42001 gatcaggata gggttccttc atgacatcca agtggacagg attctgtgag agtacctgta
    42061 gtgtcaaagg ttggataaac tctgtagcag cctgagtcat taagacagtg acttgatggc
    42121 cttgtttttt tagagaactg actaaatctg ccgacttata agaggcgatt gaacccgtta
    42181 cagccaagag aatgtttgcc atagttttcc tttctaagaa tgataggctt gaattttttc
    42241 aaggaggagt tctgcaattt cttctttagt ctggactgtt tgaagctgat ttttctcaac
    42301 aaatatagct cggtgctgat ctgctgaaat ttgagtcagg tcattcgcga tgattaaatc
    42361 tgcttgattc ttgataagac tttttcgtgc aatgtcaacc agatgatctt cggtaacatc
    42421 aaccagcagt ttgaaaccaa tcagatgaat agtgggattc cattccttga ctagggatat
    42481 gattttgggt gtctttttaa ggaacaaaac ctgaacctca tcagttgaag aaatcttggc
    42541 ctgatgattc tgcttgctta aaaattcttt tagattggag ctagcctgaa cttcctcaag
    42601 ccctgtcata taaacaggag tgtagtcaga aacagccatt gagtggatca agacctgata
    42661 atcctgaaca cgttcttgca tttctattag aaggtccttg gtattggtaa tttctcgaat
    42721 acttaggtta ggatgaggct ctggcttcag agctcgtttt gtcgtaatta aacaaacttc
    42781 atgccctgca gaaagcaaag tctctgtgat aattttcccc aagtgacctg tagaatggtt
    42841 agtgatagag cggacgctat cgatagcttc actggtaccg cccgatgtaa ctaaaatttt
    42901 catagcacta ttgtacacaa aaataagcgg taagtaaaat gaaagtgata aatttttggt
    42961 aaaaacgaag atttactata gtttcaaact ataaagccat ataaaattta agaaaggact
    43021 ctaaaagcct taaaaacaga gatatttacg aacgtttaga gagttttagg atgttaaaaa
    43081 tcttgttttg tgatataatg aattatcata taagaggtta gaggaatttt gaatgaaaac
    43141 agatattgaa atcgcacaga gtattgagtt gaagccaatt gttgatgttg tagagaaact
    43201 tggtatttct tatgacgatt tggagttgta cggaaagtac aaggctaaac tcagctttga
    43261 taaaattcgt gcagttgaga gcaatccagt cggtaaattg atcttggtta ctgccatcaa
    43321 cccaacacct gcaggtgaag gaaagtcgac gcttaccatt ggtcttgcgg atgccttgaa
    43381 caagattggc aagaaaacca tgattgctat ccgcgaaccg tctcttggtc cagtcatggg
    43441 gatcaagggt ggtgctgctg gtggtgggta tgcacaagtt ctgccaatgg aagacatcaa
    43501 cctccacttt actggagata tgcatgctat tacaactgcc aacaatgccc tttctgcctt
    43561 gattgacaac cacttgcacc aagggaatga gctggaaatt gatcaacgtc gtatcctctg
    43621 gaaacgcgtt gtggacttga acgaccgtgc gctccgccat gtgactgttg gtcttggtgg
    43681 tcctctaaac ggtattccac gtgaggatgg ttttgatatt acagttgctt cagaaatcat
    43741 ggcaattctt tgcttggcaa cggacatcga ggacttgaaa cgtcgtttgg cgaatatcgt
    43801 tattggttat cgctatgacc gtacgcctgt ttctgtaggt gatttgcagg ttgagggtgc
    43861 cttggctttg attttgaagg atgctattaa gccaaacttg gttcagacaa tttacggtac
    43921 acctgccttt gtacacggtg gtccatttgc caatatcgct catggctgta actctgtttt
    43981 ggcgacgaca acagcccttc acttggctga ttacactgtt actgaagctg gttttggtgc
    44041 ggaccttggt gctgagaaat tccttgatat caagacacca aacttgccaa catctccaga
    44101 tgcagttgtc attgtcgcaa cccttcgtgc ccttaagatg aatggtggtg tggctaaaga
    44161 cgctctgact gaagaaaatg tagaggcagt tcgtgcaggt tttgctaact tgaaacgcca
    44221 cgttgaaaat atccgtaagt tcggtattcc agcagttgta gctattaacg aatttgtatc
    44281 tgatacagaa gctgaaattg cagtcttaaa agaactctgt gcctcaatcg atgtaccagt
    44341 tgaattggct agtgtctggg ctgatggagc agaaggtgga gtagcacttg ccgaaacagt
    44401 tgttaagaca attgctgaaa atccagctaa ctataaacgt ttgtacgata atgccctttc
    44461 tgtccaagaa aagattgaaa aaattgtcac tgaaatctat cgtggtagca aagtgaactt
    44521 tgagaagaaa gctcaaacac aaattgctca aatcgttcaa aacggttggg acaaattgcc
    44581 aatctgtatg gctaaaactc aatacagttt ctcagacaat ccaaatgcgc ttggagcacc
    44641 agaaaacttt gaaattacca ttcgtgaatt ggtaccaaaa ttaggtgcag gcttcatcgt
    44701 tgccttaact ggtgatgtta tgaccatgcc aggtcttcca aaacgtccag cagctctcaa
    44761 catggatgtt gaaagcgatg gaactgtact aggcttgttc tagtggattg aaattgactt
    44821 taagtgagtt tggaaataat atccaagctc attttttctc agactgcaag cagttcatca
    44881 ataagattta tactcttcga aaatctcttc aaac
//
LOCUS       DBEJAQ010000010        44781 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.010, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000010 DBEJAQ010000000
VERSION     DBEJAQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 44781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.010"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..210
                     /locus_tag="ACL297_000616"
     CDS             <1..210
                     /locus_tag="ACL297_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGR0070918.1"
                     /translation="WKVRNIEAEAIRRNCERGAIFSGKIKYHEDSQFKGDHYVECYAV
                     LDNTVIARDRITVPIDPLCGKDFIE"
     gene            456..1790
                     /gene="trmFO"
                     /locus_tag="ACL297_000617"
     CDS             456..1790
                     /gene="trmFO"
                     /locus_tag="ACL297_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HGR0070919.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1874..2611
                     /gene="pyrH"
                     /locus_tag="ACL297_000618"
     CDS             1874..2611
                     /gene="pyrH"
                     /locus_tag="ACL297_000618"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGR0070920.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2620..3177
                     /gene="frr"
                     /locus_tag="ACL297_000619"
     CDS             2620..3177
                     /gene="frr"
                     /locus_tag="ACL297_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGR0070921.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3237..4091
                     /gene="cvfB"
                     /locus_tag="ACL297_000620"
     CDS             3237..4091
                     /gene="cvfB"
                     /locus_tag="ACL297_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HGR0070922.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4100..4315
                     /locus_tag="ACL297_000621"
     CDS             4100..4315
                     /locus_tag="ACL297_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HGR0070923.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4401..5369
                     /locus_tag="ACL297_000622"
     CDS             4401..5369
                     /locus_tag="ACL297_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGR0070924.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5562..6062
                     /locus_tag="ACL297_000623"
     CDS             5562..6062
                     /locus_tag="ACL297_000623"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070925.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6065..6391
                     /locus_tag="ACL297_000624"
                     /pseudo
     CDS             6065..6391
                     /locus_tag="ACL297_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6692..7803)
                     /gene="ald"
                     /locus_tag="ACL297_000625"
                     /pseudo
     CDS             complement(6692..7803)
                     /gene="ald"
                     /locus_tag="ACL297_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018852.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            7980..8549
                     /locus_tag="ACL297_000626"
     CDS             7980..8549
                     /locus_tag="ACL297_000626"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0070926.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8617..9267
                     /locus_tag="ACL297_000627"
     CDS             8617..9267
                     /locus_tag="ACL297_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HGR0070927.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            9251..11491
                     /locus_tag="ACL297_000628"
     CDS             9251..11491
                     /locus_tag="ACL297_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGR0070928.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11633..11857
                     /locus_tag="ACL297_000629"
     CDS             11633..11857
                     /locus_tag="ACL297_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070929.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYDSYSEYPVGSGWNYGRYEIINYYSGGY"
     gene            11890..12477
                     /locus_tag="ACL297_000630"
     CDS             11890..12477
                     /locus_tag="ACL297_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0070930.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            12481..13662
                     /locus_tag="ACL297_000631"
     CDS             12481..13662
                     /locus_tag="ACL297_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0070931.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQKIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    13822..13953
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            13969..14499
                     /gene="infC"
                     /locus_tag="ACL297_000632"
     CDS             13969..14499
                     /gene="infC"
                     /locus_tag="ACL297_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGR0070932.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14532..14732
                     /gene="rpmI"
                     /locus_tag="ACL297_000633"
     CDS             14532..14732
                     /gene="rpmI"
                     /locus_tag="ACL297_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGR0070933.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14784..15143
                     /gene="rplT"
                     /locus_tag="ACL297_000634"
     CDS             14784..15143
                     /gene="rplT"
                     /locus_tag="ACL297_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGR0070934.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15201..15581
                     /locus_tag="ACL297_000635"
     CDS             15201..15581
                     /locus_tag="ACL297_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGR0070935.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15831..16631
                     /locus_tag="ACL297_000636"
     CDS             15831..16631
                     /locus_tag="ACL297_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGR0070936.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            16642..17580
                     /locus_tag="ACL297_000637"
     CDS             16642..17580
                     /locus_tag="ACL297_000637"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGR0070937.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKVTNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17622..19391
                     /locus_tag="ACL297_000638"
     CDS             17622..19391
                     /locus_tag="ACL297_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGR0070938.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKSDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKTTNENAAYYQVVPVTANVYDSDG
                     EKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDG
                     HRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSA
                     EELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKN
                     NFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYAS
                     DPYWGEKIASVMMKINEKLGGKD"
     gene            complement(19452..21107)
                     /gene="pavA"
                     /locus_tag="ACL297_000639"
     CDS             complement(19452..21107)
                     /gene="pavA"
                     /locus_tag="ACL297_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HGR0070939.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIGLDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21212..21709
                     /gene="ybeY"
                     /locus_tag="ACL297_000640"
     CDS             21212..21709
                     /gene="ybeY"
                     /locus_tag="ACL297_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGR0070940.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHKLNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21690..22085
                     /locus_tag="ACL297_000641"
     CDS             21690..22085
                     /locus_tag="ACL297_000641"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGR0070941.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22102..23001
                     /gene="era"
                     /locus_tag="ACL297_000642"
     CDS             22102..23001
                     /gene="era"
                     /locus_tag="ACL297_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGR0070942.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23050..23874
                     /gene="mutM"
                     /locus_tag="ACL297_000643"
     CDS             23050..23874
                     /gene="mutM"
                     /locus_tag="ACL297_000643"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HGR0070943.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            23874..24479
                     /gene="coaE"
                     /locus_tag="ACL297_000644"
     CDS             23874..24479
                     /gene="coaE"
                     /locus_tag="ACL297_000644"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGR0070944.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24466..25665
                     /locus_tag="ACL297_000645"
     CDS             24466..25665
                     /locus_tag="ACL297_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HGR0070945.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25665..25814
                     /gene="rpmG"
                     /locus_tag="ACL297_000646"
     CDS             25665..25814
                     /gene="rpmG"
                     /locus_tag="ACL297_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGR0070946.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            25854..26087
                     /gene="secG"
                     /locus_tag="ACL297_000647"
     CDS             25854..26087
                     /gene="secG"
                     /locus_tag="ACL297_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGR0070947.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26189..28543
                     /gene="rnr"
                     /locus_tag="ACL297_000648"
     CDS             26189..28543
                     /gene="rnr"
                     /locus_tag="ACL297_000648"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGR0070948.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNH
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYVDVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28506..28973
                     /gene="smpB"
                     /locus_tag="ACL297_000649"
     CDS             28506..28973
                     /gene="smpB"
                     /locus_tag="ACL297_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGR0070949.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            28989..29849
                     /gene="tehB"
                     /locus_tag="ACL297_000650"
     CDS             28989..29849
                     /gene="tehB"
                     /locus_tag="ACL297_000650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HGR0070950.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            29922..30875
                     /gene="coiA"
                     /locus_tag="ACL297_000651"
     CDS             29922..30875
                     /gene="coiA"
                     /locus_tag="ACL297_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HGR0070951.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            30894..32696
                     /gene="pepF"
                     /locus_tag="ACL297_000652"
     CDS             30894..32696
                     /gene="pepF"
                     /locus_tag="ACL297_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGR0070952.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            32698..33411
                     /locus_tag="ACL297_000653"
     CDS             32698..33411
                     /locus_tag="ACL297_000653"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGR0070953.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33478..34419
                     /gene="prsA"
                     /locus_tag="ACL297_000654"
     CDS             33478..34419
                     /gene="prsA"
                     /locus_tag="ACL297_000654"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HGR0070954.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34536..34877)
                     /locus_tag="ACL297_000655"
                     /pseudo
     CDS             complement(34536..34877)
                     /locus_tag="ACL297_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            35291..35386
                     /locus_tag="ACL297_000656"
                     /pseudo
     CDS             35291..35386
                     /locus_tag="ACL297_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(35640..36260)
                     /locus_tag="ACL297_000657"
     CDS             complement(35640..36260)
                     /locus_tag="ACL297_000657"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGR0070955.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(36260..36742)
                     /locus_tag="ACL297_000658"
     CDS             complement(36260..36742)
                     /locus_tag="ACL297_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HGR0070956.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(36742..37584)
                     /locus_tag="ACL297_000659"
     CDS             complement(36742..37584)
                     /locus_tag="ACL297_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HGR0070957.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(37679..38479)
                     /locus_tag="ACL297_000660"
     CDS             complement(37679..38479)
                     /locus_tag="ACL297_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGR0070958.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            38623..40002
                     /gene="glmU"
                     /locus_tag="ACL297_000661"
     CDS             38623..40002
                     /gene="glmU"
                     /locus_tag="ACL297_000661"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGR0070959.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            40012..40557
                     /locus_tag="ACL297_000662"
     CDS             40012..40557
                     /locus_tag="ACL297_000662"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGR0070960.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            40569..40883
                     /gene="macP"
                     /locus_tag="ACL297_000663"
     CDS             40569..40883
                     /gene="macP"
                     /locus_tag="ACL297_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HGR0070961.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            40900..41592
                     /locus_tag="ACL297_000664"
     CDS             40900..41592
                     /locus_tag="ACL297_000664"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGR0070962.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            41669..42160
                     /gene="rocS"
                     /locus_tag="ACL297_000665"
     CDS             41669..42160
                     /gene="rocS"
                     /locus_tag="ACL297_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HGR0070963.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            42258..42848
                     /locus_tag="ACL297_000666"
     CDS             42258..42848
                     /locus_tag="ACL297_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGR0070964.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            42857..44620
                     /locus_tag="ACL297_000667"
     CDS             42857..44620
                     /locus_tag="ACL297_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HGR0070965.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT        13549 a         8201 c         9951 g        13080 t
ORIGIN      
        1 tggaaagtaa gaaatattga agctgaggct attagaagaa attgtgagcg tggtgctatt
       61 ttttcaggta aaataaaata tcacgaagat tcacagttta aaggagatca ctatgttgaa
      121 tgttatgctg ttttagataa tacggttata gcaagagata gaataacagt ccctatcgat
      181 ccgttatgtg gaaaagattt tatagagtag catataattg attcttaact ggaatactct
      241 ttatctcttt acatcaagaa aatgactaaa cagggaagtt tgccttcttc cctttttttg
      301 ttatactagt agaagaaaaa aatagaaaga tttgtgggag tgaaaaagtc ctgtggactt
      361 tttcagcctg agccaagaaa ctcgaaagct cgtaagtctg attggctttt tcaatgtgaa
      421 tcttaacttc atactcccaa agaggtatta gtgtcgtgtc tcaatcttat atcaatgtta
      481 tcggtgctgg tttggcaggt tctgaagcag cctaccaaat cgcagagcgt ggtattccag
      541 ttaaacttta tgaaatgcgt ggtgtcaagt caacacctca gcacaaaaca gacaattttg
      601 ctgagttggt ttgttccaat tccttgcgtg gagatgcttt gacaaatgct gttggcctcc
      661 ttaaggaaga aatgcgtcgc ttgggttctg tgattttgga atctgctgag gctacacgtg
      721 ttcctgcagg tggtgccctt gcagtggacc gtgatggttt ctctcaaatg gtgaccgaaa
      781 aagttgccaa ccaccccttg attgaagtgg ttcgtgatga aattacagaa ttgccgacag
      841 atgttattac ggttatcgct actggtcctt tgacaagtga tgccttggct gaaaagattc
      901 atgcccttaa tgacggtgat ggtttttatt tctacgatgc ggcagcgcct attatcgatg
      961 tcaacactat cgatatgagc aaggtctacc tcaaatcacg ttatgataag ggagaagcgg
     1021 cctacctcaa tgcccctatg accaagcaag aatttatgga tttccatgaa gctttggtca
     1081 atgcagaaga agcaccgctt aattcttttg aaaaagaaaa gtactttgaa ggatgtatgc
     1141 ctatcgaagt catggccaaa cgtggcatta aaaccatgct ttatggtcct atgaaaccag
     1201 ttggtcttga gtatccggac gactacacag gacctcgtga tggagaattt aaaacacctt
     1261 atgcggttgt ccagctccgt caggataatg cagctggtag cctatacaat attgttggtt
     1321 tccagaccca cctcaaatgg ggagaacaaa agcgtgtctt ccaaatgatt ccgggtcttg
     1381 aaaatgcgga gtttgtccgt tatggtgtca tgcatcgcaa ttcttacatg gattcaccaa
     1441 atcttcttga gcagacttac cgttccaaga aacaaccaaa tctcttcttt gctggtcaga
     1501 tgacgggggt ggaaggctat gttgagtcgg cggcttcggg cttagttgcg ggaattaacg
     1561 cagctcgtct cttcaaggaa gaaagcgagg ttattttccc cgagacgaca gcgattggaa
     1621 gcttagctca ttacattacc catgccgaca gcaaacattt ccaaccaatg aatgtcaatt
     1681 ttgggatcat caaggagttg gaaggcgagc gtatccgtga taagaaggct cgttatgaaa
     1741 aaattgcaga gcgtgccctt gccgacttag aggaattttt gactgtctaa tttttttgaa
     1801 agaattgctc atgatactat aaaaatctta gaaattgtga taaaataggt aggatgaaag
     1861 aaggagagtg aaaatggcga atcccaagta taaacgtatt ttaatcaagt tatcaggtga
     1921 agcccttgcc ggtgaacgtg gcgtagggat tgatatccaa acagttcaaa caatcgcaaa
     1981 agagattcaa gaagttcata gcttaggtat cgaaattgcc cttgttattg gtggaggaaa
     2041 tctttggcgt ggagaacctg cagcagaagc aggtatggac cgtgttcagg cagattacac
     2101 tggaatgctt gggactgtta tgaatgctct tgtgatggca gattcattgc aacaagttgg
     2161 ggttgatacg cgtgtacaaa cagctattgc tatgcaacaa gtggcagagc cttatgtccg
     2221 tggacgtgcc cttcgtcacc ttgaaaaagg ccgtatcgtt atctttggtg ctggaattgg
     2281 ttcaccatac ttctcgacag atacaacagc ggcccttcgt gcagctgaaa tcgaagcaga
     2341 tgccatcctc atggctaaaa atggcgtcga tggtgtgtac aatgccgatc ctaagaagga
     2401 caagacagcc gttaagtttg aagaattgac ccaccgtgat gttatcaaca aaggtcttcg
     2461 tatcatggac tcaacagcct caaccctctc aatggacaac gacattgact tggttgtctt
     2521 caacatgaac caatcaggca acatcaaacg tgtcgtattt ggtgaaaata tcggaacaac
     2581 agtttcaaat aatatcgaag aaaaggaata agaaagaata tggctaacgt aattattgaa
     2641 aaagctaaag agagaatgac ccaatctcac caatcacttg ctcgtgaatt tggtggtatc
     2701 cgtgctggtc gtgccaatgc aagcttgctt gaccgtgtac atgtagaata ctatggagtc
     2761 gaaactcctc ttaaccaaat cgcttcaatt acgattccag aagcgcgtgt tttgttggta
     2821 acaccatttg acaagtcttc attgaaagac atcgaacgtg ccttgaacgc ttctgatctt
     2881 ggtatcacac cggctaatga cggttctgtg attcgcttgg ttatcccagc tcttacagaa
     2941 gaaactcgtc gtgaccttgc taaagaagtg aagaaggtcg gcgaaaatgc taaagtggct
     3001 gtccgcaata tccgtcgcga tgctatggac gaagctaaga aacaagaaaa agcaaaagaa
     3061 atcactgaag acgaattgaa gactcttgaa aaagatattc aaaaagtaac agacgatgct
     3121 gttaaacaca tcgacgacat gactgctaac aaagagaaag aacttttgga agtctaaaaa
     3181 taaacagaaa aactcagttg gcattgctgg ctgagtttta ttcgaaagaa ggaaatatga
     3241 atacaaatct tgcaagtttt atcgttggac tgatcatcga tgaaaatgac cgtttttact
     3301 ttgtgcaaaa ggatggtcaa acctatgctc ttgctaagga agaaggtcaa catacagtag
     3361 gggatacggt caaaggtttt gcatacacgg atatgaagca aaaactccgc ctgacaacct
     3421 tagaagtgac tgccactcag gaccaatttg gttggggacg tgtcacagag gttcgtaagg
     3481 atttgggtgt ctttgtggat acaggccttc ctgacaagga aatcgttgtg tcactcgata
     3541 ttctccctga gctcaaggaa ctctggccta agaagggcga ccaactctac atccgtcttg
     3601 aagtggataa gaaagaccgt atctggggcc tcttggctta tcaagaagac ttccaacgtc
     3661 ttgctcgtcc tgcctacaac aacatgcaga accaaaactg gccagccatt gtttaccgtc
     3721 tcaagctgtc aggaactttt gtttacctac cagaaaataa tatgcttggt tttattcatc
     3781 ctagcgagcg ttacgcagag ccacgtttgg ggcaagtatt agatgcgcgc gttattggtt
     3841 tccgtgaagt ggaccgcact ctgaacctct ccctcaaacc acgctccttt gaaatgttgg
     3901 aaaacgatgc tcagatgatt ttgacttatt tggaaagtaa tggcggtttc atgaccttaa
     3961 atgataagtc atctccagac gacatcaagg caacctttgg catttctaaa ggtcagttca
     4021 agaaagcttt aggtggtctt atgaaggctg gtaaaatcaa gcaggaccag tttgggacag
     4081 agttgattta gggaggctta tgagaaaatc attttacact tggctcatga ccgagcgcaa
     4141 tcctaaaagt aacagtccca aagcaatttt ggcagacctc gcttttgaag aggcagcttt
     4201 tccaaaacat acagatgatt ttgatgaggt cagtcgcttt ttggaggagc atgccagttt
     4261 ctcttttaac ctaggagatt ttgacagcat ttggcaggaa tatctagaac actagcattt
     4321 attcattggg tttgggctag taatttctcc atccctctgc tataataaaa agaaataaaa
     4381 ggattagaga ggttctttat ttgaaggaac attcaataga cattcaactg agtcatccag
     4441 atgacctgtt tcatcttttt ggttccaatg aacgccatct tcgtttgatg gaagaagagc
     4501 ttgatgttgt gattcatgct cgtacggaga ttgtccaggt tttgggagaa gagtctgcct
     4561 gtgaggaagc ccgtcaagtt attcaggctt tgatggtctt ggtaaatcgt gggatgaccg
     4621 ttggtacgcc agatgtagtc actgcgatta gcatggtcaa aaatgatgaa attgacaagt
     4681 ttgtcgccct ttacgaagaa gaaattatca aagataatac tgggaaacct atccgtgtca
     4741 aaaccctagg gcaaaagctt tatgtggaca gtgtcaaaca gcatgatgtg acctttggaa
     4801 ttgggccagc aggtacaggg aagaccttcc ttgcagtgac cttggcagtg actgccctta
     4861 aacgtgggca agtcaagcga attatcctaa ctcgtccagc ggtggaagcg ggagagagtc
     4921 ttggatttct tccgggtgat cttaaggaga aggtggatcc ttaccttcgt cctgtttacg
     4981 atgccttgta tcagattctg gggaaagacc aaacaactcg actcatggag cgtgaaatta
     5041 tcgagattgc gccccttgcc tatatgcgtg gacggacctt ggatgatgcc tttgtcattc
     5101 tcgatgaggc gcaaaatacg accatcatgc agatgaagat gttcttgact cgtttaggtt
     5161 ttcattctaa gatgattgtc aatggagata ttagtcagat tgacctgcca cgtaatgtca
     5221 agtccggttt gattgatgct caagagaaac tcaagaacat ccatcagatt gactttgttc
     5281 atttttcagc caaggatgtg gttcgccatc cagttgtcgc tcagattatc cgagcctatg
     5341 aatattctac tgaagttgca cacgactgat tttgaggaag tttgcctgca aaagaatata
     5401 cttttgagag aggaaaatcc agttgtatag gctaaaggtt ttatccaaag gtttgagaca
     5461 acgattaggc acgatggaaa gaacttttat gtggctgatg acgatcagtg catcttcctg
     5521 tgtcataatc acagggcaca agaaagtagg aatttgaaaa gatgattgac caactatcta
     5581 agtattacag ttgtaggata ctaactgaaa aggatattcc aagtatttta tctttatatg
     5641 aaagtaatcc tctgtatttt cagcattgtc caccagagcc aaattttgca actgtaaaag
     5701 aggacatgct ttgtctacct gaaggtaaag ctaaggctga taagtttttt gttggatttt
     5761 ggaatggatc tgaccttgtg gctgttatag attttgtcta tgcacatcct gatgaggaga
     5821 ctgtttttat tggtttgttt atggttgatc aagcctatca gaggaaaggg attggtagtc
     5881 atattgtgac agaagcacta gcttattttg ctaagaactt tcgaaaggca cgtttggctt
     5941 atgttaaggg aaatccgcaa tctcagcatt tttgggaaaa gcagggcttt aaatcaattg
     6001 gatgcgaggt taagcaagaa ctctatacgg ttgttatcgc tgaacagagc ctagaagatt
     6061 agaaatggca tcaagtaaga actatttgga atttgttttg gaacaattat caggattaga
     6121 tgatgtgact taccgttcca tgatggggga gtatattctt tacttccgcg gcaagattat
     6181 tggcggcatt tatgacgatc gctttttagt taaacccgtg caagcagtct tagataagat
     6241 tgaccaatct tcttttgagt ttccatacaa aggtgccaaa gaaatgattt gagtggaaga
     6301 acttgataat aagatgtttc tataagacct gattttagct atgtataacc aactgccaac
     6361 gcccaaacct aaaaagaaaa agcaagggtg aacgaagtaa aaaagaagtc tgctaaggcc
     6421 ctgtctttgc acgggtaaaa ttttatatat aaaaagaagc tgggactaaa gagctcagct
     6481 tcctttggtt tatataattg tcattacaag acgaagtggt tgggcgaaac tctgttgact
     6541 ttattcaatt tagagtttct tatgcacaat tgagtctgga acgaaagtct ccagttgcaa
     6601 agtatacagt acaataaacc aacgatgtaa tagctgatga cacaaagcac agtgggtagg
     6661 acttgcgaag tcaccctttt cttttcaaaa tttatactaa atcattgata tcagtgtagt
     6721 cacgattaag tccttgagca actggtaggc tagtcaagta accttgataa gtggtcacac
     6781 cttgacgcaa gccttcatct tcagagattg cttgtgcgaa tcctttgcca gccaaagctt
     6841 cgatataagg aagagtgaca ttggttaggg cgatggttga agtgcgggca accgcaccag
     6901 ggatattggc aacggcatag tggagaacac cgtgtttttc atagacgggt tcatcgtgcg
     6961 ttgtcacacg gtcagctgtt tcgataacgc caccttggtc aacagcaacg tcaacgatac
     7021 agagcctgga cgcatttgtt tgaccatctc atctgtcacc aattccggtg cttttgcacc
     7081 agggatgaga atggctccaa tcaccacatc agcatctctc atacttgctt caatgttgaa
     7141 tgaattagat ataagagttt gaatttgact tccaaagact tcttctagaa ctgagagacg
     7201 cttggcacta atatctaaaa tagtcacttg agcaccaaga ccaagggcga tgcgggcagc
     7261 atgtgtaccg acgacaccat caccgatgat agttactttt ccttttggaa cacctggtac
     7321 accaccaggt agaacaccag agccaccagc ttgcttagta aggaagtgag ctccgattta
     7381 aacagccata cgacctgcaa cctcactcat aggaacgagg agcggtagtt gtccttgatt
     7441 gtcacgaaca gtttcatagg caattcctgt tgtttttgct gctaacatag catctgctaa
     7501 ttctggagca gcggccatgt gcaagtaggt gaagagaaga agatcgtcgc gcaagtaacc
     7561 gtattcagaa cttaaaggtt cttttacttt cacaaccaac tctgctgccc aagcttcacc
     7621 agcagtagcg acaatctcag ctccttgctt ttgatagtca gcatcagtaa agccagaacc
     7681 gagaccagca tttgtttcga taaggacacg atgaccacga ctaactaagc tatgaacacc
     7741 tgcaggtgtg agggcgacac ggttttcgtt atttttaatt tcttttggga ttccgattaa
     7801 cattgagata acctaccttt caattgacgg tcttgttttg gttgtcacat tccagttcat
     7861 aaatcaaaaa tgtgacggtt tcattgtata tgaaaccgct tcaaaaatca agaaaaactt
     7921 gtcatccaaa tttttttatg ctagactagt gaaaatcaag ctctaatgga gggaaaagta
     7981 tggaatcaat atttgtgaaa tttgcccagt atccgtctat agaaacggag cgtttattgc
     8041 ttagacctgt aactttggat gatgcggaag caatgtttga ctatgcctcg gacaagggta
     8101 atacacgtta cacttttcca accaatcaaa gcttggaaga aaccaagaat aacattgctc
     8161 agttctactt ggctaatccc ttgggacgtt gggggattga attaaaagat aacggcaagt
     8221 ttatcggaac tattgactta cacaagattg atcctgttct taagaaggca gctattggct
     8281 acattatcaa taaaaagtat tggaatcaag gattaacgcc agaaaccaat cgtgctgtga
     8341 ttgagctagc ttttgagaag ataggaatga ataagttagt tgctttatac gataaggaca
     8401 attctgcgtc aggaaaggtc atggagaaat caggcatgcg tttttcccat gcagaaccat
     8461 atgcttgtat ggaccagcat gaaaaaggcc gaatcgtgac aagagttcat tatgtcttga
     8521 ccaaggaaga ctattttgca aataaataag cagttgaaaa gaaatttttc gactgttttt
     8581 tcttcctctt acgaataatc taagagagga gaaaatatgg aagcaattat cgagaaaatc
     8641 aaagagtata aaatcatcgt catctgtact ggtctgggct tgcttgtagg cggatttttc
     8701 ctgctaaagc cagctccaca aacacctgta aaggaaacga atttgcaggc tgaagttgca
     8761 gctgtttcca aggatttggt atccgaaaag gaagtgaaca aggaagagaa ggaagaaccc
     8821 cttgaacaag atctaatcac agtagatgtc aaaggtgctg tcaaatcgcc agggatttat
     8881 gacttgcctg taggtagtcg agtcaatgat gctgttcaga aggctggtgg cttgacagag
     8941 caagcagaca gcaagtcgct caatctagct cagaaagtta gtgatgaggc tctggtttac
     9001 gttcctacta agggagaaga agcagttagt caacagactg gttcggggac agcttcttca
     9061 ataagcaagg aaaagaaggt caatctcaac aaggccagtc tggaagaact caagcaggtc
     9121 aagggactgg gaggaaaacg agctcaggac attatcgacc atcgtgaggc aaatggcaag
     9181 ttcaagtcag tagacgagct caagaaggtc tctggcattg gtggcaaaac aatagaaaag
     9241 cttaaagact atgttacagt ggattaagaa tttctctatt cccctaattt acctgagttt
     9301 tctattactt tggctttatt acgctatttt ctcagcatct tatcttgctt tgttgggctt
     9361 tgtttttctg ctagtctgtc tctttatcca atttccgtgg aaatctgctg gtaaagttct
     9421 aataatttgc ggaatctttg gattttggtt tgtttttcaa aattggcaac agagtcaagc
     9481 gagtcaaaat ctggcggatt ctgttgaaag ggtacggatt ttgcctgata ctattaaggt
     9541 taatggtgat agtctatcct ttcgtggcaa gtctaacggt cgtgctttcc aagtctatta
     9601 taaactccag tccgaggagg agaaagaagc ctttcaagct ttaaccgacc tgcatgagat
     9661 aggactagaa gggaagcttt cggagccaga agggcagaga aattttggtg gctttaatta
     9721 ccaagcctat ctgaagactc agggaattta ccagactctc aatatcaaaa caatccagtc
     9781 acttcaaaag attggcagtt gggatatagg agaaaacttg tccagtttac gtcgaaaggc
     9841 tgtggtttgg attaagacgc actttccaga ccctatgcgc aattacatga caggactctt
     9901 gctgggacat ctggacaccg actttgagga gatgaatgag ctttattcca gtctaggaat
     9961 tatccacctc tttgccctat ctggtatgca ggtaggtttt ttcatgaatg gatttaagaa
    10021 acttctcttg cgattgggct tgacccaaga aaagttgaaa tggctgactt atcccttttc
    10081 ccttatctat gcgggactaa ctggattttc agcatcggtt attcgcagtc tcttgcaaaa
    10141 gctactggct caacatgggg ttaagggctt ggataatttt gccttgacgg tgcttgtcct
    10201 ctttattgtc atgccaaact ttttcttgac agcaggagga gtcttgtcct gcgcttatgc
    10261 ttttatcctg accatgacca gcaaagaagg ggaggggctc aaggctgtta ctagtgaaag
    10321 tctagtcatc tccttgggca tattgcccat tctatccttc tattttgcgg aatttcaacc
    10381 tttgtctatc cttttgacct ttgtcttttc ctttctattt gacttgacct tcttaccgct
    10441 cttgtctatt ttatttgtcc tttcctttct ctatccagtc attcagctga actttatctt
    10501 tgaatggttg gagggcatta ttcgcttggt gtcacaggtg acaagtagac ctctggtctt
    10561 tggacaaccc aatgcatggt ttttaatcct attgttaatt tccttggctt tggtctatga
    10621 tttgagaaaa aacattaaaa agctaacggt attgtgctta ttgattacag ggctctttct
    10681 cctgaccaag catccactgg aaaatgaaat caccatgctg gatgtggggc aaggagaaag
    10741 tattttccta cgggatgtaa ctgggaaaac cattctcata gatgtaggtg gtaaggcaga
    10801 atcttataag aaaatcgaaa aatggcaaga aaaaatgacg accagcaatg cccagcgaac
    10861 cttgattccc tatctcaaaa gtcgaggagt agctaagatt gaccagctaa ttttgactaa
    10921 cacggacaag gagcatgttg gagatttgtc agaggtgacc aaggctttcc atgtagggga
    10981 gattctagta tcaaaagaca gtctgaaaca gaaggaattt gtggcagaac tacaggcgac
    11041 tcaaacaaag gtgcgtagta tgacagtagg ggagaacttg cccatttttg gaagtcagtt
    11101 agaagttcta tctccaagga aaatgggaga tggaggacac gatgataccc tagttctgta
    11161 tgggaaattc ttggataagc aatttctctt cacgggaaat ttggaggaga aaggagagaa
    11221 ggacttgctg aagcactatc cagacttgaa agtaaatgtt ttgaaagcta gccaacatgg
    11281 caataaaaaa tcatcaagtc cagcctttct agaaaaactc aaaccagagc ttactcttat
    11341 ctcagttgga aagagcaatc gaatgaaact cccccatcag gaaacattga cacgactgga
    11401 aggtatcaat agcaaagttt atcgaactga ccagcaagga gctatacgtt ttaaggggtt
    11461 ggatagttgg aaaatcgaaa gtgttcgata ggaaggataa atgttgtaga ttagtgaaat
    11521 aaactaaaaa tttgttgcat aataatgata aaaatggtat aatgaaaacg tattcaatat
    11581 tgaggatata aaatcattaa aaattagcaa aaatcgttta attagttagt ttatgattat
    11641 tggtcttttt cagtccagta tatctgctgt gacagtcact aaaagttaca agtatgattg
    11701 gaatacggtt tgggaatata gtaccaacta tcacgaccat cagtatgctt ggattccgtc
    11761 atggtctcgt tatgacagct attctgagta tccagttggt tctggctgga actacggtcg
    11821 ttatgagatc ataaactatt acagcggagg ctattaattc ttaaagagtg agaaaaagga
    11881 gggctagata tgttgcagct tactcatgtg accttaaaaa cgcgacaagt catcttgcaa
    11941 gatgtggatt tcacctttaa aaagggtagg gtttatggtc ttcttgctat caatggctct
    12001 ggaaagacga ccctgttccg tgccattagc aatttaattc ccataagtag tggaaatatc
    12061 gcagcccctc cttctttatt ttattatgag agtattgaat ggctggatgg aaacttaagt
    12121 gggatggact accttcgtct tatcaaaaac atctggaagt caggtctgaa cttgagggat
    12181 gaaatcgcct attgggaaat gtctgactat atcagtcttc ccattcgcaa gtattcctta
    12241 ggcatgaagc aacgcttggt gattgccatg tatttcctca gtcaggccaa atgctggctc
    12301 atggatgaga ttacaaatgg cttagatgag tattatcgac agaagttttt tgataggcta
    12361 gcacaaatcg atagacaaga acagctggtt cttttaagtt cccactataa ggaagagttg
    12421 gttgatgtct gcgatagagt agtaaccatt catcaggggc agatagaaga ggtttagttt
    12481 atgaaagatg ttagtctatt tttattgaaa aaagttttca aaagccgctt aaactggatt
    12541 gtcttagctt tatttgtatc tgtactcggt gttacctttt atttaaatag tcagactgca
    12601 aactcacaca gcttggagag caggttggaa agtcgcattg cagccaacga gagggctatc
    12661 aatgaaaatg aagagaaact ctcccaaatg tctgatacca gctcggagga ataccagttt
    12721 gctaaaaata atttagacgt gcaaaaaaat cttttgacgc gaaagacaga aattctaact
    12781 ttactaaaag aagggcgctg gaaagaagcc tactatttgc agtggcaaga tgaagagaag
    12841 aattatgaat ttgtatcaaa tgacccgact gctagctctg gcttaaaaat gggggttgac
    12901 cgcgaacgga agatttacca agccctgtat cccttgaaca taaaagcaca tactttagag
    12961 tttccgaccc acgggattga tcagattgtc tggattttag aggttatcat cccaagcttg
    13021 tttgtggttg cgattatttt tatgctaaca caactatttg cagaaagata tcaaaatcat
    13081 ctggacacag ctcacttata tcctgtttca aaagtgacat ttgcaatatc ctctcttgga
    13141 gttggagtgg gatatgtaac tgtgctgttt atcggaatct gtggcttttc ttttctagtg
    13201 ggaagtctga taagtggttt tggacagtta gattatccct acccgattta tagcttagtg
    13261 aatcaagagg taactattgg aaagatgcag gatgtattat ttcctggctt gctcttagct
    13321 ttcttagcct ttatcgtcat tgtggaagtt gtgtatttga ttgcttactt tttcaagcaa
    13381 aaaatgcctg tcctctttct ttcactcatt gggattgttg gcttattgtt tggcatccaa
    13441 accattcagc ctcttcaaaa gattgcacat ctgattccct ttacttactt gcgttcagtg
    13501 gagattttat ctggaagatt acctaagcag attgataatg tcgatctaaa ttggagcatg
    13561 gggatggtct tacttccttg cctgattatc cttttgttag tggggattct atttatcgaa
    13621 agatggggaa gttcacgtaa aaaggaagtt tttaaagttt agttttccta tagggaagct
    13681 aaagaaaaac taacataggg agagaatcaa cttggttctc tctttttgat tcgaaaacca
    13741 aaccaaaata caaacacaaa cttttcaaaa aataactttt tatcttgaca agagctagaa
    13801 aacttggtat catataaaag ttgagaaaag cagaagtgag agcttctcgc cttgtgacat
    13861 taagttgcct ggccctacgg atgaaaagtt tcgaagaaac gctatcataa cgtgcgggct
    13921 tgtatattta cgagtccgct attgtttttc tctaataaaa caaaagaggt gaaaaccata
    13981 gcaaagcaag acttattcat caatgatgag attcgtgtac gtgaagttcg cttgattggt
    14041 cttgagggag aacagctagg catcaagcca ctcagtgaag cgcaagcttt ggctgataac
    14101 gctaatgttg acctagtatt gattcaaccc caagccaaac cgcctgttgc aaaaattatg
    14161 gactacggta agttcaaatt tgagtaccag aagaagcaaa aagaacaacg taaaaaacaa
    14221 agcgttgtta ctgtgaaaga agttcgtcta agtccaacaa ttgacaaggg tgactttgat
    14281 acaaaacttc gtaatgcacg taagttcctt gaaaaaggaa ataaagttaa ggtatctatt
    14341 cgctttaagg gtcgtatgat tacccataaa gagattggtg caaaagtttt agccgagttt
    14401 gctgaagcaa ctcaagatat tgccatcatc gaacaacgtg ctaaaatgga tggacgtcaa
    14461 atgttcatgc aattggcgcc agcgactgac aaaaaataat tgtcagaaag ttaaataaag
    14521 gagaaaaaat catgccaaaa caaaaaacac accgcgcatc agctaaacgt ttcaaacgta
    14581 caggttctgg tggacttaaa cgtttccgtg cttacacttc tcaccgtttc cacggaaaaa
    14641 ctaagaaaca acgtcgtcat cttcgtaaag catctatggt gcattcagga gattacaaac
    14701 gtatcaaagc aatgcttact cgcttgaaat aatagcttga ctgtaagtaa acaattctag
    14761 aaaatatttg gaggaaataa aacatggcac gtgttaaagg tggcgttgta tcacgcaaac
    14821 gtcgtaaacg tattcttaaa ttagcaaaag gttactatgg agctaaacac atcttgttcc
    14881 gtactgcaaa agaacaagta atgaactctt actactatgc ataccgtgac cgtcgtcaga
    14941 aaaaacgtga cttccgcaaa ttgtggatta ctcgtatcaa tgcggcggct cgtatgaacg
    15001 gactttcata ctcacaattg atgcatggtt tgaaattggc tgagatcgaa gttaaccgta
    15061 aaatgcttgc tgacttggct gttaacgatg cagtagcttt cacagctctt gcagatgcag
    15121 ctaaagcaaa acttggtaaa taacaactga tgagactgga acaggattgt ccagtctttt
    15181 taaaaataaa ggagaaagat atggcttcaa aaatgctaca cacttgctta cgagtagaaa
    15241 atcttgaaaa atcaatcgca ttttatcaag atgcttttgg ttttaaagaa ttgcgtcgca
    15301 gagattttcc agaccatgcc ttcacgattg tctatctagg tcttgagggt gatgattatg
    15361 agttggagtt gacttataac tacgatcacg gtccgtatgt ggttggtgat gggtttgccc
    15421 atatcgccct cagtacacct gatcttgagg cacttcatca agaacacagt gcaaaaggct
    15481 atgaagtgac tgagccaaat ggtctaccag gaactacacc aaactattac tttgttaaag
    15541 accctgatgg atataaggtc gaagtcattc gtgaaaaata atttcgtgca agttaagtag
    15601 aatattcgtt ttctacttga ctttttttag ctgaaagagt atactaataa aaattcaacc
    15661 tttaagggga gtccagagag actcacaagg tgtcagataa aagaatagtg caattttcta
    15721 gaggagacct tttgagtgtg ctctcttgtg ttgtacgatt ttaactgagg ccttgcacta
    15781 gcaaggtctt ttctttatct ggtcccctta aaatttaagg aggaaaagtt atgaatctca
    15841 catgtaagaa gcgtttgggt gtcattcggt tggaaaccat gaaggtggtt gcacaagagg
    15901 aaatcgcgcc agcaatcttt gaattagtcc tagaaggaga aatggttgaa gccatgcgag
    15961 caggccaatt tcttcatctg cgtgtaccag acgatgccca tctcttacgt cgtcctattt
    16021 caatttcgtc tattgacaag gcaaacaagc agtgtcacct catttatcgg attgagggag
    16081 ctgggactgc aattttttca accttaagtc agggagacac tcttgatgtg atggggcctc
    16141 agggaaatgg ttttgacttg tctgaccttg atgagcagaa tcaggttctc cttgttggtg
    16201 gtgggattgg tgttccaccc ttgcttgagg tggccaagga attgcatgaa cgtggagtga
    16261 aagtagtgac agtcctcggt tttgctaata aggatgctgt tattttgaaa acggaattgg
    16321 ctcagtatgg tcaggtcttt gtaacgacag atgatggttc ttatggcatc aagggaaatg
    16381 tttccgttgt tatcaatgat ttagacagtc agtttgatgc tgtttactcg tgtggggctc
    16441 caggaatgat gaagtatatc aatcaaacct ttgatgatca cccaagagcc tatttatctc
    16501 tggaatctcg tatggcttgt gggatgggag cttgctatgc ctgtgttcta aaagtaccag
    16561 aaagcgagac ggtcagccaa cgcgtctgtg aagatggtcc tgttttccgc acaggaacag
    16621 ttgtattata aggagaaaat tatgactaca aatcgattac aagtttctct acctggtttg
    16681 gatttgaaaa atccgattat tccagcatca ggctgttttg gctttggaca agagtatgcc
    16741 aagtactatg atttagacct tttaggttct attatgatca aggcgacaac ccttgaacca
    16801 cgttttggaa atccaactcc aagagtggca gagacgcctg ctggtatgct caatgcaatt
    16861 ggcttgcaaa atcctggttt agaggttgtt ttggctgaaa agctaccttg gctggaaaga
    16921 gaatatccaa atcttcctat tattgccaat gtagctggtt tttcaaaaca agagtatgca
    16981 gctgtttctc atgggatttc caaggtaact aatgtaaaag ctatcgagct caatatttct
    17041 tgtcccaatg ttgaccactg taatcatgga cttttgattg gtcaagatcc agatttggct
    17101 tatgatgtgg tgaaagcagc tgtggaagcc tcagaagtgc cagtttatgt caaattaacc
    17161 ccgagtgtga ccgatatcgt tactgtcgca aaagctgcag aagatgcggg agcaagtggc
    17221 ttgaccatga tcaatactct ggttggaatg cgctttgacc tcaaaaccag aaaaccaatc
    17281 ttggccaatg gaacaggtgg aatgtcaggt ccagcagtct ttccagtagc cctcaaactc
    17341 atccgccaag tagcccaaac aacagacctg cctatcattg gaatgggggg agtggattcg
    17401 gctgaagctg ccctagaaat gtatctggct ggggcatctg ctatcggagt tggaacagct
    17461 aactttacca atccttatgc ctgccctgac atcatcgaaa atttaccaaa agtcatggat
    17521 aaatacggta ttagcagtct ggaagaactc cgtcaggaag taaaagagtc tctgaggtaa
    17581 actgcaatca atctgttctt gattttttat tagtttgtaa tatgaattta ggagaatttt
    17641 ggtacaataa aataaataag aacagaggaa gaaggttaat gaagaaagta agatttattt
    17701 ttttagctct gctatttttc ttagctagtc cagagggtgc aatggctagt gatggtactt
    17761 ggcaaggaaa acagtatctg aaagaagatg gcagtcaagc agcgaatgag tgggtttttg
    17821 atactcatta tcaatcttgg ttctatataa aagcagatgc taactatgct gaaaatgaat
    17881 ggctaaagca aggtgacgac tatttttacc tcaaatctgg tggctatatg gccaaatcag
    17941 aatgggtaga agacaaggga gccttttatt atcttgacca agatggaaag atgaaaagaa
    18001 atgcttgggt aggaacttcc tatgttggtg caacaggtgc caaagtaata gaagactggg
    18061 tctatgattc tcaatacgat gcttggtttt atatcaaagc agatggacag cacgcagaga
    18121 aagaatggct ccaaattaaa gggaaggact attatttcaa atccggtggt tatctactga
    18181 caagtcagtg gattaatcaa gcttatgtga atgctagtgg tgccaaagta cagcaaggtt
    18241 ggctttttga caaacaatac caatcttggt tttacatcaa agaaaatgga aactatgctg
    18301 ataaagaatg gattttcgag aatggtcact attattatct aaaatccggt ggctacatgg
    18361 cagccaatga atggatttgg gataaggaat cttggtttta cctcaagtct gatggcaaaa
    18421 tggctgaaaa agaatgggtc tacgattctc atagtcaagc ctggtactac ttcaaatctg
    18481 gtggctacat ggcgaaaaat gagacagtag atggttatca gcttggaagc gatggtaaat
    18541 ggcttggagg aaaaactaca aatgaaaatg ctgcttacta tcaagtagtg cctgttacag
    18601 ccaatgttta tgattcagat ggtgaaaagc tttcctatat atcgcaaggt agtgtcgtat
    18661 ggctagataa ggatagaaaa agtgatgaca agcgcttggc tattactatt tctggtttgt
    18721 caggctatat gaaaacagaa gatttacaag cgctagatgc tagtaaggac tttatccctt
    18781 attatgagag tgatggccac cgtttttatc actatgtggc tcagaatgct agtatcccag
    18841 tagcttctca tctttctgat atggaagtag gcaagaaata ttattcggca gatggcctgc
    18901 attttgatgg ttttaagctt gagaatccct tccttttcaa agatttaaca gaggctacaa
    18961 actacagtgc tgaagaattg gataaggtat ttagtttgct aaacattaac aatagccttt
    19021 tggagaacaa gggcgctact tttaaggaag ccgaagaaca ttaccatatc aatgcccttt
    19081 atctccttgc ccatagtgcc ctagaaagta actggggaag aagtaaaatt gccaaagata
    19141 agaataattt ctttggcatt acagcctatg atacgacccc ttacctttct gctaagacat
    19201 ttgatgatgt ggataaggga attttaggtg caaccaagtg gattaaggaa aattatatcg
    19261 ataggggaag aactttcctt ggaaacaagg cttctggtat gaatgtggaa tatgcttcag
    19321 acccttattg gggcgaaaaa attgctagtg tgatgatgaa aatcaatgag aagctaggtg
    19381 gcaaagatta gtactataag tgaatatgat ttgagtgaat agtaagttaa aaatcctgat
    19441 ttcaagtaaa atcaggattt tttcatggat gcaatttttt tggagtctgg tgtgacgcgg
    19501 agggtctttt gtcctgtgta agtgacaaag ccgggttttc caccagttgg tttattgagt
    19561 tttttgactt caatcatatc tacctgcacc agattcgaca ggcgcccttg agagaagtag
    19621 gcagctaact ctgctgcgtc tgtcttgact gcatcagatg ggtcaagatt tcctgagatg
    19681 acaacatggc ttccaggaat gtccttagca tggaaccaaa gttcctcctt gcgggccatt
    19741 ttaaaggtca attcctcatt ttgaagattg tttcgtccga catagatgat ggttttgcca
    19801 tcgcttgcta gatattgttc tagttttttg cgtttctgga ttttctcccg ttgtcttctg
    19861 cggataaaac ctgtttgaat caattcttca cggatttcag cgatttcttc cagtccagct
    19921 tggttgagga cggtttctac actttccaga tagagaatag tggctttggt ttcttcaatc
    19981 aaatcagtca agtatttgac agcttctttg agtttctgat accgtttaaa atagcgttgg
    20041 gcattctggt tgggagtcag agccttatca agcccaatca tgataggttg gttggtatag
    20101 tagttgtcta ggataacctg gtcttggtcg ttaggcactt ggtggaggaa ggttgtcagc
    20161 aattctcctt tttgacgaaa ttcttcagcg ttgtctgtcg ccagtaattc tttttcctgt
    20221 tttttgagtt tgtgtcggtt tttctgaagt tcattttcaa cacgacgaat cagttcactg
    20281 gcctgctgtt tgacgcggtc gcgctcagcc ttatccttat agtaggtgtc caacaaatca
    20341 gaaagatttg caaaaggctc tcccacctga tttgcaaaag gaactggact gaaggaagtc
    20401 tcagtcaagc atggcttggt ttcctgattg aaaaaatttc ggaaagcgga aagtttttca
    20461 ctaaccagta tcctttccaa ttcatttgcc gtatcgcgtc ccagaccttg aaagaggctt
    20521 tgaagatttt ttgctgttag ttcttgggtt tgcaggattt caaagagctt ttcatccttg
    20581 atagtaaaag gattgagaga ttctgtactt ggcggagcga tataggtcga tcctggaagt
    20641 aaggtgcggt agctattttg tgaaaagccg acgtgtttga taacttcgag gattttatga
    20701 ctgcttttat cgaccagtag aatattactg tgtttcccca taatttcgat aatcaaggta
    20761 gcctggatat ggtctccaat ctcgttttta ttggaaactg tcatttccac aatacggtca
    20821 ttttccactt gctcaatcga ctcaatcagg gccccctgca aatactttct caaaaccatg
    20881 ataaaggtag aaggttgggc tggattttca aaagtcgttt gggtcagctg aatgcgtcca
    20941 aaaactggat gggcagaaag gagcaggcga tggctttggc gattgctgcg gatttgcaag
    21001 accaactctt gttcaaaagg ctgattgatt ttctggatgc gaccattcac taattcgctt
    21061 cgcaattcct caactatgtg gtgtaaaaaa aatccgtcaa atgacatcgt tctctccttg
    21121 tgattgtatt ccatagtatt atatcaaaaa ggtagaataa aatcatggaa atgtggtata
    21181 ataaagccaa gtaaagagaa acgagaagca catgtatatt gaaatggtag atgaaactgg
    21241 tcaagtttca aaagaaatgt tgcaacaaac ccaagaaatt ttggaatttg cagccaaaaa
    21301 attaggaaaa gaagacaagg agatggcagt cacttttgtg accaatgagc gtagtcataa
    21361 acttaatctg gagtaccgtg acaccgaccg tccgacagat gtcatcagcc ttgagtataa
    21421 accagaattg gaaattgcct ttgacgaaga ggatttgctt gaaaatccag aattggcaga
    21481 gatgatgtct gagtttgatg cctatattgg ggaattgttc atctctatcg ataaggctca
    21541 tgagcaggcc gaagaatatg gtcacagctt tgagcgtgag atgggcttct tggcagtaca
    21601 cggcttttta catattaacg gctatgatca ctatactccg gaagaagaag cggagatgtt
    21661 cggtttacaa gaagaaattt tgacagccta tggactcaca agacaataaa cgaaaatgga
    21721 aaaatcgtga cctgatatcc agtttagaat ttgccatcac agggattttt actgctatca
    21781 aggaagaacg caatatgcga aaacatgcag tgacggctct agtggtcatc cttgcaggtt
    21841 ttggttttca ggtgtcacga atcgaatggc tctttctcct attgagcatt ttcttggtgg
    21901 tagcctttga aattatcaat tctgctattg aaaatgtggt ggatttggcc agtcactatc
    21961 acttttccat gctggctaaa aatgccaagg atatggcggc tggcgcggta ttagtggttt
    22021 ctctttttgc agccttaaca ggcgcattga tttttctccc acgaatctgg gatttattat
    22081 tttaaacagt aagaggaaat tatgactttt aaatcaggct ttgtagccat tttaggacgt
    22141 cccaatgttg ggaagtcaac ctttttaaat cacgttatgg ggcaaaagat tgccatcatg
    22201 agtgacaagg cgcagacaac gcgcaataaa atcatgggaa tttacacgac tgataaggag
    22261 caaattgtct ttatcgacac accagggatt cacaaaccta aaacagctct cggagatttc
    22321 atggttgagt ctgcctacag tacccttcgc gaagtggaca ctgttctttt catggtgcct
    22381 gctgatgaag cgcgtggtaa gggggacgat atgattatcg agcgtctcaa ggctgccaag
    22441 gttcctgtga ttttggtggt gaataaaatc gataaggtcc atccagacca gctcttgtct
    22501 cagattgatg acttccgtaa tcaaatggac tttaaggaaa ttgttccaat ctcagccctt
    22561 cagggaaata acgtgtctcg tctagtggat attttgagtg aaaatctgga tgaaggtttc
    22621 caatatttcc cgtctgatca aatcacagac catccagaac gtttcttggt ttcagaaatg
    22681 gttcgcgaga aagtcttgca cttaactcgt gaagagattc cgcattctgt agcagtagtt
    22741 gttgactcta tgaaacgaga cgaagagaca gacaaggttc acatccgtgc aaccatcatg
    22801 gtcgagcgcg atagccaaaa agggattatc atcggtaaag gtggcgctat gcttaagaaa
    22861 atcggtagca tggcccgtcg tgatatcgaa ctcatgctag gagacaaggt cttcctagaa
    22921 acctgggtca aggtcaagaa aaactggcgc gataaaaagc tagatttggc tgactttggc
    22981 tataatgaaa gagaatacta agtagaggta ggctcatgcc tgcttcttgt ttttacagaa
    23041 ggaggactta tgcctgaatt acctgaggtt gaaaccgttt gtcgtggctt agaaaaattg
    23101 attataggaa agaagatttc gagtatagaa attcgctacc ccaagatgat taagacggat
    23161 ttggaagagt ttcaaaggga attgcctagt cagattatcg agtcaatggg acgtcgtgga
    23221 aaatatttgc ttttttatct gacagacaag gtcttgattt cccatttgcg gatggagggc
    23281 aagtattttt actatccaga ccaaggacct gaacgcaagc atgcccatgt tttctttcat
    23341 tttgaagatg gtggcacgct tgtttatgag gatgttcgca agtttggaac catggaactc
    23401 ttggtgcctg accttttaga cgcctacttt atttctaaaa aattaggtcc tgaaccaagc
    23461 gaacaagact ttgatttaca ggtctttcaa tctgcccttg ccaagtccaa aaagcctatc
    23521 aaatcccatc tcctagacca gaccttggta gctggacttg gcaatatcta tgtggatgag
    23581 gttctctggc gagctcaggt tcatccagct agaccttccc agactttgac agcagaagaa
    23641 gcgactgcca ttcatgacca gaccattgct gttttgggcc aggctgttga aaaaggtggc
    23701 tccaccattc ggacttatac caatgccttt ggggaagatg gaagcatgca ggactttcat
    23761 caggtctatg ataagactgg tcaagaatgt gtacgctgtg gtaccatcat tgagaaaatt
    23821 caactaggtg gacgtggaac ccacttttgt ccaaactgtc aaaggaggga ctgatgggaa
    23881 aaatcatcgg aatcactggg ggaattgcct caggtaagtc aactgtgaca aattttctaa
    23941 gacagcaagg ctttcaagca gtggatgccg acgcagtcgt ccaccaacta cagaaacctg
    24001 gtggtcgtct gtttgaggct ctagtacagc actttgggca agaaatcatt cttgaaaacg
    24061 gagaactcaa tcgccctctc ctagctagtc tcatcttttc aaatcctgaa gagcaaaaat
    24121 ggtctaatca aattcaaggg gagattatcc gtgaggaact ggctactttg agagaacagt
    24181 tggctcagac agaagagatt ttcttcatgg atattcccct actttttgag caggactaca
    24241 gcgattggtt tgctgagact tggttggtct atgtggaccg agatgcccaa gtagaacgct
    24301 taatgaaaag ggaccagttg tccaaagatg aagctgagtc tcgtctggca gcccagtggc
    24361 ctttagaaaa aaagaaagat ttggccagcc aggttcttga taataatggc aatcagaacc
    24421 agcttcttaa tcaagtgcat atccttcttg agggaggtag gcaagatgac agagattaac
    24481 tggaaggata atctgcgtat tgcctggttt ggtaattttc tgacaggagc cagtatttct
    24541 ttggttgtac cttttatgcc catcttcgtg gaaaatctag gtgtagggag tcagcaagtc
    24601 gctttttatg caggcttagc aatttctgtc tctgctattt ccgcggcgct cttttctcct
    24661 atttggggta ttcttgctga caaatacggc cgaaaaccca tgatgattcg ggcaggtctt
    24721 gctatgacta tcactatggg aggcttggcc tttgtcccaa atatctattg gttaatcttt
    24781 cttcgtttac taaacggtgt atttgcaggt tttgttccta atgcaacggc actgatagcc
    24841 agtcaggttc caaaggagaa atcaggctct gccttaggta ctttgtctac aggcgtagtt
    24901 gcaggtactc taactggtcc ctttattggt ggctttattg cagaattatt tggcattcgt
    24961 acagttttct tactggttgg tagttttcta tttttagctg ctattttgac tatttgcttt
    25021 atcaaggaag attttcaacc agtagccaag gaaaaggcta ttccaacaaa ggaattattt
    25081 acctcggtta aatatcccta tcttttgctc aatctctttt taaccagttt tgtcatccaa
    25141 ttttcagctc aatcgatcgg ccctattttg gctctttatg tacgcgactt agggcagaca
    25201 gagaatcttc tttttgtctc tggtttgatt gtgtccagta tgggcttttc cagtatgatg
    25261 agtgcaggag tcatgggcaa gctaggtgac aaggtgggca atcatcgtct cttggttgtc
    25321 gcccagtttt attcagtcat catctatctc ctctgtgcca atgcctctag cccccttcaa
    25381 ctaggactct atcgtttcct ctttggcttg ggaaccggtg ccttgattcc cggggttaat
    25441 gccctactca gcaaaatgac tcccaaagcc ggcatttcga gggtctttgc ctttaatcag
    25501 gtattctttt atctgggagg tgttgttggt cccatggcag gttctgcagt agcaggtcaa
    25561 tttggctacc atgctgtctt ttatgcgaca agcctttgtg ttgcctttag ttgtctcttt
    25621 aacctgattc aatttcgaac attattaaaa gtaaaggaaa tctagtgcga gtaaaaatta
    25681 atctcaaatg ctcctcttgt gacagtatca attacctaac cagtaaaaat tcaaaaaccc
    25741 atccagacaa gattgaggtt ttaaagtatt gtcccaagga aagaaaagta accctacatc
    25801 ttgaatctaa gtagatttta tggtaaaata atagggattt taaggagttt gatatgtata
    25861 acctattatt aaccatttta ttagtattat ctgttgtgat tgtgattgca attttcatgc
    25921 aaccaaccaa aaaccaatcc agcaatgtat ttgatgccag ttcaggtgat ttgtttgaac
    25981 gcagtaaagc tcgcggtttt gaagctgtaa tgcagcgttt gacagggatt ttagtctttt
    26041 tctggctagc cattgcctta gcattgacgg tattatcaag tagataagaa aataatgggc
    26101 aggactaggt ctttgcctct ttttattttt aaaggatgtt tgagaaggtt ttacagtaaa
    26161 agaaaattaa aaaatctaga aagaaaatat gaaagataga ataaaagaat atttacaaga
    26221 caagggaaag gtgactgtta atgatttggc tcaggctttg ggaaaagaca gttccaagga
    26281 ttttcgtgag ttgattaaaa ccttgtcctt aatggaaaga aagcaccaga ttcgctttga
    26341 agaagatggt agtctgacat tagaaattaa gaaaaaacat gagattaccc tcaaggggat
    26401 ttttcatgcc cataaaaatg gctttggctt tgttagtctg gaaggcgagg aggacgacct
    26461 ttttgtaggg aaaaatgatg tcaactatgc tattgatggt gataccgtcg aggtagtgat
    26521 taagaaagtc gctgaccgca ataagggaac agcagcagaa gccaaaatta ttgatatcct
    26581 agaacacagt ttgacaacag ttgtcgggca aatcgttctg gatcaggaaa agcctaagta
    26641 tgctggctat attcgttcaa aaaatcataa aatcagtcaa ccgatttatg ttaagaaacc
    26701 agccctaaaa ttagaaggaa cagaagttct caaagtcttt atcgataaat acccaagcaa
    26761 gaaacatgat ttctttgtcg ctagtgtact cgatgtagtg ggacactcaa cggatgtcgg
    26821 aattgatgtt cttgaggtct tggaatcaat ggacattgta tctgagtttc cagaagctgt
    26881 tgtcaaggaa gcagaaagtg tgcctgatgc tccgtctcaa aaggatatgg aaggtcgtct
    26941 ggatctaaga gatgaaatta cctttaccat tgacggtgcg gatgccaagg acttagacga
    27001 tgcagtgcat atcaaggctc tgaaaaatgg caatctggag cttggggttc acatcgcaga
    27061 tgtttcctat tatgtgaccg aggggtctgc ccttgacaag gaagccctta accgtgcgac
    27121 ttctgtttac gtgacagacc gagtggtgcc aatgcttcca gaacgactat caaatggcat
    27181 ctgctctctc aatccccaag ttgaccgcct gacccagtct gctattatgg agattgataa
    27241 acatggtcgt gtggtcaact ataccattac acaaacagtt atcaagacca gttttcgtat
    27301 gacctatagc gatgtcaatg atatcctagc tggcgatgaa gaaaagagaa aagaatatca
    27361 taaaattgta ccaagtatcg aactcatggc caagcttcat gaaactttag aaaacatgcg
    27421 tgtgaaacgt ggagctctca attttgatac caatgaagcg aagattttag tggataaaca
    27481 aggtaagcct gttgatatcg ttcttcggca gcgtggcatt gccgagcgga tgattgagtc
    27541 ttttatgttg atggctaatg aaacagttgc cgaacatttc agcaagttgg atttgccttt
    27601 tatctatcga attcacgagg agcctaaggc tgaaaaggtt cagaagttta ttgattatgc
    27661 ttcgagtttt ggcttgcgca tttatgggac tgccagtgag attagtcagg aggcacttca
    27721 agacatcatg cgtgctgttg agggagaacc ttatgtagat gtattgtcca tgatgcttct
    27781 tcgctctatg cagcaggctc gttattcgga gcacaatcac ggccactatg gactagctgc
    27841 tgactattat actcacttta ccagtccaat tcgtcgttat ccagaccttc ttgttcaccg
    27901 tatgattcgg gattacggct gttctaagga aatagcagag cattttgaac aagtgattcc
    27961 agagattgcg acccagtctt ccaaccgtga acgtcgtgcc atagaagctg agcgtgaagt
    28021 cgaagccatg aaaaaggctg agtatatgga agaatacgtg ggtgaagagt atgatgcagt
    28081 tgtatcaagt attgtcaaat tcggtctctt tgtcgaattg ccaaacacag ttgaaggctt
    28141 gattcacatc actaatctac ctgaatttta tcatttcaat gagcgtgatt tgactcttcg
    28201 tggagaaaaa tcaggtatca ctttccgagt gggtcagcag atccgtatcc gtgttgaaag
    28261 agcggataaa atgactggag agattgattt ttcattcgta cctagtgagt ttgatgtgat
    28321 tgaaaaaggc ttgaaacagt ctagtcgtag tggcagaggg cgtggttcaa atcgtcgttc
    28381 ggataagaag gaagacaaga gaaaatcagg acgctcaaat gataagcgta agcattcaca
    28441 aaaagacaag aagaaaaaag gaaagaaacc tttttacaag gaagtagcta agaaaggagc
    28501 caagcatggc aaagggcgag ggaaaggtcg tcgcacaaaa taaaaaggca cgccacgact
    28561 atacaatcgt agatacgcta gaggcaggga tggtcctgac tggaactgaa atcaagagtg
    28621 tacgagctgc tcgaattaat ctcaaggatg gctttgctca agtgaaaaat ggagaagttt
    28681 ggctgagcaa tgttcatatc gcgccttacg aagagggcaa tatctggaac caggaaccag
    28741 aacgtcgtcg taaactcctg ctccataaaa agcaaattca aaaattggaa caagagacca
    28801 aagggacagg aatgacctta gttcccctta aggtctatat aaaagatggc tacgctaagc
    28861 ttcttttagg acttgccaaa gggaagcatg actatgacaa acgggagtct atcaaacgtc
    28921 gtgagcaaaa tcgagatatc gcgcgtgtga tgaaagctgt taatcagcga taaaaagagg
    28981 aattgaaaat ggaaaaatta gttgcctata aacgcatgcc tttgtggaat aaacaaacaa
    29041 tgcctgaagc tgttcagcaa aagcacaata caaaagttgg gacttggggg aaaattactg
    29101 tcttgaaggg agctctcaag tttattgaat tgacagaaga aggggaagtt ctagctgaac
    29161 acctctttga agcaggggca gacaatccaa tggcccaacc tcaagcctgg caccgagtgg
    29221 aagctgccac agatgatgtg gaatggtact tggaatttta ttgtaaacct gaggattatt
    29281 ttgctaaaaa atacaatacc aatcctgttc attcagaggt cctagaggcc atgcagacag
    29341 tgaaacaagg gaaagctttg gatttgggtt gtggtcaggg gcgtaattct ctttttctag
    29401 cccagcaaga ttttgatgtg acggctgtag atcaaaatgg actagctctt gaaatcttgc
    29461 aaagcattgt ggagcaggaa gatttggaca tgcctgttgg cctttacgat atcaattcag
    29521 ctagcattga acaagaatat gattttatcg tttcaacagt tgttctcatg tttctacaag
    29581 cggaccgcat tccagctatt attcaaaata tgcaggagaa aaccagtgtt ggcggttaca
    29641 accttatcgt ttgtgccatg gacacggagg attatccttg ctcggttaac ttcccattca
    29701 cctttaaaga aggagaactg gcagactatt acaaggattg ggaattggtt aagtacaatg
    29761 aaaatccagg ccatttgcac cgtcgcgatg agaatggcaa tcgtattcaa ctacgctttg
    29821 cgaccttact agctaagaaa atcaagtaaa cacacatgaa gattaggaat tttcctgatc
    29881 ttttttcttt tttacgaatg atatagaaaa ggagggaatt catgtttgtt gcgagagatg
    29941 ctaggggaga attggtaaat gtgttagagg ataaacttga gaagcaagca tacacctgcc
    30001 cagcttgtgg aggccagctc catttgcgtc aaggaccaag tgtacggacg cattttgccc
    30061 ataaatcctt aaaagactgt gattttttct ttgaaaatga aagtccagaa cacctggcca
    30121 ataaggaatc cctctatcac tggttgaaaa aagagacaaa ggttcaatta gagtacccgc
    30181 tttcagaact taaacagatt gcggatgtat ttgtaaatgg caatctagct ctagaagttc
    30241 agtgtagtcc cttgcctcag aaagtcctta aagagcgaag tgagggctat cgtagtcagg
    30301 gttaccaagt actgtggttg ctgggtcaaa aactgtggct caaggagcgt ttgactcgtc
    30361 tacagcaagg ttttctttat ttcagtcaaa acatgggctt ttatgtttgg gaattagaca
    30421 aggaaaaaca agttttaaga ctcaaatacc tgatttacca ggatctccgc ggtaaactcc
    30481 attatcaaat caaggaattt tcctatggtc aaggtagttt attggaaata ttgcgtcttc
    30541 cctataagag acaaaaaata tctcatttta cagtttctga ggacaaggac atctgtcgct
    30601 atatccggca acaactttat tatcaaaatc tcttttggat gaaagaacaa gcagaagcct
    30661 atcaaaaggg agaaaatatc ctgacttatg gactgaaaga atggtatcca caaattcgac
    30721 caatagtggg caaatttttc cagattgaac aagacttgac tagctattat cagcactttt
    30781 atacctatta ccaaaaaaat cctcaaaatg attggcaaaa gctttatcca ccagcctttt
    30841 atcagcaata tttcttgaaa aatatggtag aatagaaagg atggaggaat ctaatggtat
    30901 tacaaagaaa tgaaataaat gaaaaagata catgggatct atcaacgatc tacccaactg
    30961 accaggcttg ggaagaagcc ttaaaagatt taacagaaca attggagaca gtagcccagt
    31021 atgaaggcca tctcttggat agtgcggata acctactaga aatcactgaa ttttctcttg
    31081 aaatggaacg ccagatagag aagctttacg tttatgctca tatgaagaat gaccaggata
    31141 cacgtgaagc taagtatcaa gagtactatg ccaaggccat gacactctac agccagttag
    31201 accaagcctt ttcattctat gagcctgaat ttatggagat tagcgaaaag cagtatgctg
    31261 actttttaga agctcaacca aagctgcagg tttatcaaca ctattttgac aagctcttgc
    31321 aaggcaagga tcacgttctt tcacaacgtg aagaagaatt attggctgga gctggagaaa
    31381 tctttggttc agcaagtgaa accttcgcta tcttggacaa tgcggatatt gtgttccctt
    31441 atgtcctaga cgatgatggt aaagaagttc agctatctca tgggacttac acacgtttga
    31501 tggagtctaa aaaacgtgag gttcgccgtg gtgcctatca agctctttat gctacttacg
    31561 aacaattcca acacacctat gccaaaacct tgcaaaccaa tgttaaggtg caaaattacc
    31621 gtgctaaagt tcgtaactac aagagtgctc gtcatgcagc cctcgcagcg aattttgttc
    31681 cagaaagtgt ttatgacaat ttggtagcag cagttcgcaa gcatttgcca ctcttacatc
    31741 gctatcttga gcttcgttca aaaatcttgg ggatttcaga tctcaagatg tacgatgtct
    31801 acacaccgct ttcatctgtt gaatacagtt ttacctacca agaagccttg aaaaaagcag
    31861 aagatgcttt ggcagtcttg ggtgaggatt acttgagccg tgttaaacgt gccttcagcg
    31921 agcgttggat tgatgtttac gaaaatcaag gcaagcgttc aggtgcctac tctggtggtt
    31981 cttacgatac caatgccttt atgcttctca actggcagga caatctggac aatctcttta
    32041 ctcttgttca tgaaacaggt cacagtatgc attcaagcta tactcgtgaa actcagcctt
    32101 atgtttacgg ggattactct atctttttgg ctgagattgc ctcaactacc aatgaaaata
    32161 tcttgacgga gaaattattg gaagaagtgg aagacgacgc aacacgcttt gctattctca
    32221 ataacttcct agatggtttc cgtggaacag ttttccgcca aactcaattt gctgagtttg
    32281 aacacgccat tcaccaagca gatcaaaatg gggaggtctt gacaagcgat ttcctaaata
    32341 aactctacgc agacttgaac caagagtatt atggtttgag taaggaagac aatcctgaaa
    32401 tccaatacga gtgggctcgc attccacact tctactataa ctactatgta tatcaatatt
    32461 caactggctt tgcggccgcc tcagccttgg ctgaaaaaat tgtccatggt agtcaagaag
    32521 accgtgaccg ctatatcgac tacctcaagg caggtaagtc ggactatcca cttaatgtca
    32581 tgagaaaagc tggtgttgat atggagaagg aagactacct caacgatgcc tttgcagtct
    32641 ttgaacgccg tttaaatgag tttgaagccc ttgttgaaaa attaggattg gcataaaatg
    32701 gttgaatcgt atagtaagaa tgctaaccat aacatgcgtc gtcctgtcgt caaagaagaa
    32761 attgtagact tgatgcgtca gcgtcaaaag caggtcacag gtttcttgaa agaattggaa
    32821 gactttgccc gcaaggaaaa tattcctatt attccccatg aaacggttgc ttatttccgt
    32881 tttcttatgg aaaccatgca gcctaaaaat attctggaaa ttgggacggc tatcggtttt
    32941 tcagctctct tgatggctga acatgcgcca aatgctaaga ttacaactat tgatcgtaat
    33001 ccagaaatga ttggttttgc caaggaaaat tttgcccagt ttgacagtcg caagcaaatc
    33061 actctcctag agggagatgc ggtggatgtc ttatctacac tgacagagtc ttatgatttc
    33121 gtatttatgg attctgccaa gtctaaatac atcgtctttc tgccagaaat cctcaaacat
    33181 ttggaagttg gtggtgtggt tgtcttggat gatatttttc aaggtggtga tgttgccaag
    33241 gatattatgg aagtccgtcg tggtcagcga accatttatc gaggccttca aaaattattt
    33301 gatgcaacct tagacaatcc agaactcacc gcaacattag tgcctttagg agatggtatt
    33361 ctcatgcttc gtaaaaatgt agcagatgtt caactgtctg aaagcgaatg attttcagaa
    33421 aaatttaaga aaaaatagta aaatagatag agtaacactt atctcaaagg agtagacatg
    33481 aagaaaaaat tattggcagg tgccatcaca ctattatcag tagcaacttt agcagcttgt
    33541 tcgaaagggt cagaaggagc agaccttatc agcatgaaag gggatgtcat cacagaacat
    33601 caattttatg agcaagtgaa aaacaaccct tcagcccaac aagtcttgtt aaatatgacc
    33661 atccaaaaag tttttgaaaa acaatatggc tcagagcttg atgataaaga ggttgatgat
    33721 actattgccg aagaaaaaaa acaatatggc gaaaactacc aacgtgtctt gtcacaagca
    33781 ggtatgactc ttgaaacacg taaagctcaa attcgtacaa gtaaattagt tgagttggca
    33841 gttaagaagg tagcagaagc tgaattgaca gatgaagcct ataagaaagc ctttgatgag
    33901 tacactccag atgtaacggc tcaaatcatc cgtcttaata atgaagataa ggccaaagaa
    33961 gttctcgaaa aagccaaggc agaaggtgct gattttgctc aattagccaa agataattca
    34021 actgatgaaa aaacaaaaga aaatggtgga gaaattacct ttgattctgc ttcaacagaa
    34081 gtacctgagc aagtcaaaaa agccgctttc gctttagatg tggatggtgt ttctgatgtg
    34141 attacagcaa ctggcacaca agcctacagt agccaatatt acattgtaaa actcactaag
    34201 aaaacagaaa aatcatctaa tattgatgac tacaaagaaa aattaaaaac tgttatcttg
    34261 actcaaaaac aaaatgattc aacatttgtt caaagcatta tcggaaaaga attgcaagca
    34321 gccaatatca aggttaagga ccaagccttc caaaatatct ttacccaata tatcggtggt
    34381 ggagattcaa gctcaagcag tagtacatca aacgaatagt ccaaatcaat gagtcaggga
    34441 aaaaactcta cttcaggaaa aaatgaagca aacattccca caataaaacg cgtagtacaa
    34501 ggtttgtact gacccccaaa agttagacaa ataatttatc cgaaggattt agttctgtat
    34561 tgcacagggc taagtccttt tagttttacc ttaattctct tgttgttgta ataatcaata
    34621 tagtctataa tggcttgttc caattgatta agtgatttaa atgttttctc atagccataa
    34681 aacatttcgg attttaaaat accaaagaaa gattccatcc taccgttgtc ttggctgttt
    34741 cccttgcgtg acatagatgc ttgaattccc ttactctcta ggaaccgatg ataagaatcg
    34801 tgttggtatt gccagtcttg gtcactatgg agaatcgtat tctcgtagtg cttctctttg
    34861 aatgcctgtt ccaacattaa cgatcaatca atttaatcat gtacctaata ttagaattgt
    34921 ttatctcaaa tttatttgaa agcttctcta agctatatcc ttgttttcta agttcataga
    34981 tctgaacttt atcatcataa gttaatttca taataaaaac actccaaaag ttagattttt
    35041 tctgtctaac ttttggggtg cagttcattt aacacctgat aatatgcgtt ttttttattt
    35101 taaagacttt ttgaccagcc tcatttttta acttgatact cagtgaaaag caaagattaa
    35161 actaggaagc tagctgtagg ctgctcaaag aacagctttg aggttgcaga taaaacttgt
    35221 gaggtaacca acatatataa tgtgaagctg acgtggtttg aatagatttt agaagggtat
    35281 gagtctggaa gttttaatgg ataatgcaag attccataga atgggtaagc tagagttctt
    35341 atgtgaagag tttgggcata aacttttacc ttttcctccc tactcatctt agtatagaaa
    35401 agtgaatcta aaatagtaca taactgcttc taaaacattc ttataaattg atttaaattc
    35461 tcaaatcata ttattcagtt cttatttcat tttgctctac aatcctgttg agaagacacg
    35521 tgttcatatc aaaaaaggta ttggcaagtt gcaatacctt tttacgaggc tcttttatct
    35581 tatttttgtt tcaactgact atatctccta tggttctagt tcagaaggct aggctataat
    35641 tatgattgat aagaagtatc attccaagta ttgagagtga atgtttcaaa atcatgggtt
    35701 tctataatgg tcaggctggc atttgctaga ccgccatctt tacgaagaag tggttcttta
    35761 tagcctagga gagtacgaag actggcagta agattggcgc cgtgtccgac aattagaata
    35821 cgttcagctg gactatcttt taatgatttg ataaattgga tggtccgttg agttgtacta
    35881 tagagggatt cggctccgaa cattcgagtg tcaaattgag caagatttga acgaaaagcc
    35941 tggatttgtt gcgggtaaat agcttccaag gttgcaattt tcaaaccttc taacttccca
    36001 agttgccatt cacggagatt aggaacgatt tctaaagaac agggggtata gagttgactt
    36061 tggataatct cagcagattt gaccgctcga ggtaaatcac ttgaataaat ctgatcaaaa
    36121 ggaatttcct tgagatactg accaagtcgt tttagggttt caatggattc aggaagaaga
    36181 ggagaatcac cactagcacc ttgaaaacga ccttcttggt tccagagggt acgaccgtgg
    36241 cggacaaagt agagtttcat tacttgatgt cctccaaaat atctacaaag tctgccttta
    36301 caaagctagc caagtcttgt ggcgcgacga taatgctgtg tccgacttcg cctgcagaga
    36361 caatcatttg atccaaatct agagcaattt tatcgataaa aatgggataa ttgtgtttct
    36421 gacgaattcc gacaggatta ttggctccat gaatgtaacc agttgttttt tctaagtcct
    36481 tttgtggaat catgctcact tttttattgc cagaaatttt agctagtttc ttttcagaca
    36541 agtgctgagt gatagggaca attccgataa tcggtccggt cttgtctccc aaaagcgcca
    36601 aggttttgaa aatctgatct cgttcataac cttgaggaag ctctccttct agggcattga
    36661 tttgaatccc ctgatgaggg atagctgctt tagataggat ttgttccacc aatgtttttt
    36721 tgattttaac tttttttgcc attatttata tttatcctcc aattgactca tccaaatacc
    36781 aagccagatt cccagcgcaa agaagaaggc gatgatgaca taaccgacaa gtgaaagtcc
    36841 tgtgtattgg atactttcag cgtttcctgc atttggaatt aagatcaaaa gggtacttga
    36901 taggacgata ccgatgatga aatgatagac acgtgagtgg tagttgttta aggcataatc
    36961 catcaatttt gaaaaaacga tgagagttgc acctgcacca attccaatcg gaaagaaggt
    37021 tcccaagaaa tcaaaagttt taaaaccagt caacatagga gcatagagtc ccaaaatcaa
    37081 aagtaaattt gatgggctga ggccaggaac caagacgcca agggccaata gtgcgcctgc
    37141 taggacgaag ttaagaaagc tggcgcttaa ggttccaacg acaaaattta aggcatagag
    37201 tcctaatcca gaaatgataa aggttgtcca taaccaagct aaatcaatct tgtctcggtc
    37261 agattctcga gttgattctt tgaggaggct aggaactgta ccgataatag ctcccgcaaa
    37321 gctccataat acaaaaacct gataattttc aagcaggtat tcaatcgggt aggaaaataa
    37381 gccgattccc agaagcatac cgatggcaac tggaataaag tacaaaacat tttctttaaa
    37441 gtctttaaag ggatgggcca gaaagccaat cattcgttca tagattccta agattgctgc
    37501 tagaacccct ccggaaattc ccggtaggat aaatccaaga gcaattacaa tccctttaat
    37561 aacgcgtgct aaccatgaga gcatatattt cctccaacta tttctatttc aaaaaatcct
    37621 tactatattg tatcacaatc agacacaaaa agaaaaagca aatgataaac aaatgctttt
    37681 aaggttttaa aaaaagtttt cgaaaggttt cttctttatt ttttaaggga gagataacgt
    37741 tgatatctaa atcgtggtca aagccggcaa tttttccttt agatgtgtat tggtgaatat
    37801 cataatctaa atcagtttta ggactgctct ccaaaaatcc tgagtctgag ccgtaggacg
    37861 gaatccaaac agaggtaaac ttgcctgtat caatactgtg ttcttccatg aagtagacac
    37921 caacgtagat gccgatgttt ttagcaccta gtgatgctag ttttgctcga aagttttcga
    37981 caccttcgtt catattagac atggttttgt cttccacgtc aagccaatag taactagggc
    38041 tgtaaggaga ggcagcattg tagaaaactt cggcagcctt ttccatttct tggacacttt
    38101 ttccagctac ataagcgtag acagcaactg ggacattccg cttttgaagt tcagtgatat
    38161 gactcttata ggccttgtct attccattga taaatgaagc atcattttct tttgtcgttt
    38221 gagcaccact gtgaacacga acaatagcac ctgaaatatt ttgtgagagg gcatcgtagt
    38281 tgatttcttc aggacgctgc cagccagaga ggtcaataat cggtttgtct aagtgtttca
    38341 aagcctgtgc ttcaatctgt gctatattgg attttgtttt aaacgattgg ttgtcattaa
    38401 gtgggcgatt gatgattaaa atgaacatca taatccccaa aaaactaaat aaaataagtg
    38461 gatgaatttg ttttctcata tcttataatt ctaccctaaa aatcaaaaaa aatcaaaaaa
    38521 atgggttaag gaagagactt tagagcattt tttcattcaa gagtgcggaa tgatttgaaa
    38581 tatggtataa taaaagggaa tttctacaga aaagagaaga ttatgtcaaa ttttgccatt
    38641 attttagcag cgggtaaagg gactcgcatg aaatctgatt tgccaaaagt tttgcacaag
    38701 gttgcgggta tttctatgtt ggaacatgtt ttccgtagtg tgggagctat ccaacctgaa
    38761 aagacagtaa cagttgtagg acacaaggca gaattggttg aggaggtctt ggctggacag
    38821 acagaatttg tgactcaatc tgaacagttg ggaactggtc atgcagttat gatgacagaa
    38881 cctatcttag aaggtttgtc aggacacacc ttggtcattg caggagatac tcctttaatc
    38941 actggtgaaa gcttgaaaaa cttgattgat ttccatatca atcataaaaa tgtggccact
    39001 atcttgactg ctgaaacgga taatcctttt ggctatggac gaattgttcg taatgacaat
    39061 gctgaggttc ttcgtattgt tgagcagaag gatgctacag attttgaaaa gcaaatcaag
    39121 gaaatcaaca ctggaacata cgtctttgac aacgagcgtt tgtttgaggc tttgaaaaat
    39181 atcaatacca ataacgctca aggcgaatac tatattacag acgtcattgg tattttccgt
    39241 gaaactggtg aaaaagttgg cgcttatact ttgaaagatt ttgatgaaag tcttggggta
    39301 aatgaccgtg tggcgcttgc gacagctgag tcagttatgc gtcgtcgcat caatcataaa
    39361 catatggtca acggtgttag ctttgtcaat ccagaagcaa cttatatcga tattgatgtt
    39421 gagattgctc cggaagttca aatcgaagcc aatgttacct tgaaagggca aacgaaaatt
    39481 ggtgctgaga ctgttttgac aaacggtact tatgtagtgg acagcactat cggagcagga
    39541 gcggtcatta ccaattctat gattgaggaa agtagtgttg cagacggtgt gacagtcggt
    39601 ccttatgctc acattcgtcc aaattcaagt ctgggtgccc aagttcatat tggtaacttt
    39661 gttgaggtga aaggatcttc aatcggtgag aataccaagg ctggtcattt gacttatatc
    39721 ggaaactgtg aagtgggaag caacgttaat ttcggtgctg gaactattac agtcaattat
    39781 gacggcaaaa acaaatacaa gacagtcatt ggagacaatg tctttgttgg ttcaaattca
    39841 accattattg caccagtaga acttggtgac aattccctcg ttggtgctgg ttcaactatt
    39901 actaaagacg tgccagcaga tgctattgct attggtcgcg gtcgtcagat caataaagac
    39961 gaatatgcaa cacgtcttcc tcatcatcct aagaaccagt aggagcctat catggagttt
    40021 gaagaaaaaa cgcttagccg aaaagaaatc tatcaaggac caatatttaa actggtccaa
    40081 gatcaggttg aattaccaga aggcaaggga actgcccaac gggatttgat tttccacaat
    40141 ggggctgtct gtgttttagc agtaacggat gaacaaaaac tgatcttggt caagcagtac
    40201 cgcaaagcta tcgaggctgt ctcttacgaa attccagccg gaaaattgga agtaggagaa
    40261 aacacagccc ctgtggcagc tgcccttcgt gaattagagg aagaaacagc ctatacaggg
    40321 aaattagaac tcttgtacga tttttattca gctattggct tttgtaatga gaagttaaaa
    40381 ctatatttag caagcgattt gacaaaagtg gaaaatccgc gtccgcagga tgaggatgaa
    40441 accttggaag tccttgaagt gagcttagaa gaagcgaaag aattaatcca atcaggtcat
    40501 atttgtgatg ccaagacaat tatggctgtt cagtattggg agttgcagaa aaaatagagg
    40561 aggtcagtat gggtaaatct ttattaacgg atgaaatgat tgaaagagct aatagaggcg
    40621 aaaaaatttc aggtcctcct ttgctagatg ataatgagga gactaagatt ttaccaacct
    40681 cttcttcccg ttttggttat gccaatccta agaatcatgg ttttagccag gaaaccttga
    40741 agattcaggt cgaaccatct attcataaaa gccgtcgcat tgaaaatacc aagagaaatg
    40801 tcttcaattc taagttgaat aaaatcttat tcgcggtcat ctttctcttg attttgcttg
    40861 ttttagcaat gaaacttttg taatagaaaa ggaattgaaa tgaaaatagg aattattgct
    40921 gctatgccag aagaactggc ttatctggtc cagcatttat ataatgtcca ggagcaagtt
    40981 gttttgggga atacctatca tacaggaacc attgcttctc atgaagtcgt tcttgtagaa
    41041 agtggaattg gtaaggtcat gtctgctatg agtgtggcga ttttggctga tcatttccag
    41101 gtggatgccc ttattaatac gggttcagct ggggcagtag cagaaggtat cgctgttggg
    41161 gatgtcgtga ttgctgacaa attagcctat catgacgtgg atgtcacagc ttttggctat
    41221 gcttatggac aaatggcgca acaaccgctt tatttcgaat cagacaaaac ctttgttgct
    41281 caaatccaag agagtttatc tcaattggac caaaactggc atcttggttt gattgctaca
    41341 ggagatagtt ttgttgcagg aaatgacaag atagaagcga ttaagtccca tttcccagaa
    41401 gttttagccg tggagatgga gggggcagct attgctcaag cagcgcatac cctcaatctc
    41461 ccagtcttag tcatccgagc tatgagtgac aatgccaacc atgaagcaaa catctttttt
    41521 gatgagttta ttatcgaagc tggacgtcgc tctgcccaag tcttgttggc ctttttgaag
    41581 gctttagatt aagcggaaat ttgacagttt ttctagctta tgataagatt taagtaaaga
    41641 aaagctagaa aacgtttcag aggatattat gagtattgaa atgaccgtca gtgagattgc
    41701 agaggtctta ggattatctc gccaagcaat caataaccgt gtcaaagaat taccagaaga
    41761 agacacagat aaaaatgaca aaggggtaac agttgttacc agaagtggct tgattaagct
    41821 agaagaaatc tataaaaaaa cgatttttga agatgaacct gtcagtgaag atgtcaaaca
    41881 acgtgaactg atggagattc tagtggatga gaagaatgca gaaattcttc gtctttatga
    41941 acaattaaaa gccaaggatc gtcagttatc agaaaaagac gagcagatgc gtatcaaaga
    42001 ccgtcagatt gctgagaaag acaaacaatt agatcagcaa caacaattga cccttcaagc
    42061 catgaaggat caagaaaatc ttaagctaga gctggaccaa gcaaaagaag aagtccaatc
    42121 cactaaaaaa ggcttttttg ctcgtttatt tggaggataa tcaaggagct gtttaggtta
    42181 aatgctaacc tgatggcttc ttttgtttgt gaatagtata gttgattaga acagagacag
    42241 tagaggagag gtacagaatg gaacgattag aagattacat tgcttttgat ttagaattta
    42301 atcagcacga aggacaaaca cacttgattc aagtatcggc agtgcgtttt agagatggtc
    42361 gggaaatcga tgcctatgat tcctatgttc ataccagcgt ccctctaaaa agctttatca
    42421 atggtttgac cgggattaca gctgagacct taaaagatgc tcctccagtt gagaaagtct
    42481 tagaagaatt ccaagagttt gtgggttctt tgctgatgat aggctacaat gctgctaaaa
    42541 gtgatttacc tattttagca gagcatggtt tagactatag ccaacagtac aaggtggatt
    42601 tgtatgatga agcttttgaa cgtcggagtt cagatttaca tggtattgcc aatctcaaat
    42661 tgcaaactgt tgcctcattt cttggatttc aaggtcagtc tcataatagt ttagaggatg
    42721 ctcggatgac ggcgcgtgtt tacgaatcct tcttagagtc ggatcaagct agggaattgt
    42781 tagagacaga aagtagctta tcaaacaatc cttttggtgg cttggacttg tctcaattat
    42841 ttgactagga gtgcctatga aacctgaaaa acctaaaaaa ttgggtctta aacagattta
    42901 cctcaatctt gataaaatcc tctttctatt tttcttgatt tttatgatag tggagtattt
    42961 gtgggtacca ttcaattcat ggttggctag cttcttatta gatcagacag gttatctttt
    43021 tatttcctat aacaatgtct tagccatcct tactcattct ccattgatta gtctagcttt
    43081 tttagccttg attgtaatta actttctggt tgcttatttt caaataggac tcctctttat
    43141 tggtgctcgt catctcctct ataatgaaaa gcgaaggcta attgagtata tccgcaaggt
    43201 tttccatgaa agttttgaat ttatgaaaaa gctaactggc gctaaagcct tgtttatctt
    43261 tttctatata gctatgctgt ttccttttat acggaaaatg ctgaagattt attatcttaa
    43321 taaaatcgtc attccagagt ttatccaagc ttatttagaa gataaatatt ggatgtggtg
    43381 gctgagcatc ctacttctat ctcttatctt tctctatgtc tctgtcagat tgatgtttgc
    43441 ccttccgaag attgtatatg atcagctgac tgttcgagaa gcagtgatgt ttagcttgga
    43501 gaaaaccaag aagagagtta ctttttatgc ttggaattta ttttacatct tgctcaaagc
    43561 acatttatta ttttatcttc ctttgattcc tttattattg gctcaaactt tggtagatga
    43621 tatcactcag agagagtctc tgattcttgg tattttgaat tttgtcgtga ttaaaaatct
    43681 ctattatatg gctctgactt attttttggt taaatttgtt tcatttttga caggaaagga
    43741 gctggatatg cttcctagga gagaaaaaga tcacatcgtg cgatggggtg tcatgacttg
    43801 tgccagtttt ttctttgcct tagaaggtta tatttatctg gaggctccca tggttcatct
    43861 acctcaactt atttctcacc gaggggtttc caatgcaaat gggattcaaa atacagtaga
    43921 gtccttggaa actacagcac aactcaaacc agacttggtg gagacggacg tgcaggaaac
    43981 aaaagatggg cagtttgtca tgatgcatga tgctaacttg aaaaatctag caggtatcaa
    44041 taaaagtcct caagacttaa acttggagga gcttaaaggg attgatattt ttgaaaatgg
    44101 ctaccagact aaaatttcaa gctttgaaga ttatctcagt cgagccaacg aacttggtca
    44161 aaaattacta attgaaatta aaaccagtaa aaaagatagt ccagacatga tgaaccgctt
    44221 tttagcccgt tatgctgcaa agctcaagat ttatggacat caaatccagt ctttagacta
    44281 ccatgttgtc gaaaaagtaa gacagtatga tgccgaactg ccagtttatt tcatcatgcc
    44341 ctacaattct gtctttccta aaacaagagc gacaggatat actatggagt actcaacctt
    44401 ggatgaatat tttgtaagca aactatggac aacggatcag aaactttatg tctggactgt
    44461 caatgattca gaagctatca gcaaatctct tcacttagga gtagatggag tgattacaga
    44521 tgatttggaa aaagttcagc gggaaataga agtagcccaa gaagatccag aatatacgga
    44581 tttgctcttg aaaaaagcat tggaattctt tgaattttag taataaaaaa gaggaacggt
    44641 tgttcctccc atgataggta agtattcttg tgaatgcttg cttttttgtg gttgcaatcc
    44701 gtttacgaga attgtttgta ttgttcttgt ttcaatttac tttatagtgc tttgaagttg
    44761 gaatgctaca ctataacttc t
//
LOCUS       DBEJAQ010000011        44774 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.011, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000011 DBEJAQ010000000
VERSION     DBEJAQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 44774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.011"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            179..1942
                     /gene="aspS"
                     /locus_tag="ACL297_000668"
     CDS             179..1942
                     /gene="aspS"
                     /locus_tag="ACL297_000668"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGR0070966.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            1920..2861
                     /locus_tag="ACL297_000669"
     CDS             1920..2861
                     /locus_tag="ACL297_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGR0070967.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQSGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(2903..3088)
                     /locus_tag="ACL297_000670"
     CDS             complement(2903..3088)
                     /locus_tag="ACL297_000670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070968.1"
                     /translation="MQTRKLAAGCSKHCFEVVDRTDEVSSKHGFEVADRTDEVSSKHG
                     FEVADRTDEVSNIYTAR"
     gene            complement(3242..4228)
                     /locus_tag="ACL297_000671"
     CDS             complement(3242..4228)
                     /locus_tag="ACL297_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGR0070969.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(4238..5038)
                     /locus_tag="ACL297_000672"
     CDS             complement(4238..5038)
                     /locus_tag="ACL297_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HGR0070970.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(5291..6133)
                     /locus_tag="ACL297_000673"
     CDS             complement(5291..6133)
                     /locus_tag="ACL297_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGR0070971.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(6135..7427)
                     /locus_tag="ACL297_000674"
     CDS             complement(6135..7427)
                     /locus_tag="ACL297_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGR0070972.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(7538..8809)
                     /locus_tag="ACL297_000675"
     CDS             complement(7538..8809)
                     /locus_tag="ACL297_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGR0070973.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            9344..10876
                     /gene="malQ"
                     /locus_tag="ACL297_000676"
                     /pseudo
     CDS             9344..10876
                     /gene="malQ"
                     /locus_tag="ACL297_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            10902..13160
                     /locus_tag="ACL297_000677"
     CDS             10902..13160
                     /locus_tag="ACL297_000677"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HGR0070974.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14204..14776)
                     /locus_tag="ACL297_000678"
     CDS             complement(14204..14776)
                     /locus_tag="ACL297_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070975.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            14853..15059
                     /locus_tag="ACL297_000679"
     CDS             14853..15059
                     /locus_tag="ACL297_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070976.1"
                     /translation="MLKKYFSKYKWTDLFWILFVILTCLYIGNHDLFTLNHQEFSFRG
                     SVWGLVLALYHLLFIDKFVISNRK"
     gene            complement(15398..16246)
                     /locus_tag="ACL297_000680"
     CDS             complement(15398..16246)
                     /locus_tag="ACL297_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HGR0070977.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            16398..16676
                     /locus_tag="ACL297_000681"
     CDS             16398..16676
                     /locus_tag="ACL297_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGR0070978.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            16678..18741
                     /locus_tag="ACL297_000682"
     CDS             16678..18741
                     /locus_tag="ACL297_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGR0070979.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLVRTKELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(18819..20075)
                     /gene="tyrS"
                     /locus_tag="ACL297_000683"
     CDS             complement(18819..20075)
                     /gene="tyrS"
                     /locus_tag="ACL297_000683"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGR0070980.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            20217..22682
                     /gene="pbp1b"
                     /locus_tag="ACL297_000684"
     CDS             20217..22682
                     /gene="pbp1b"
                     /locus_tag="ACL297_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HGR0070981.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            22747..23658
                     /locus_tag="ACL297_000685"
     CDS             22747..23658
                     /locus_tag="ACL297_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGR0070982.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            23888..24586
                     /gene="dapD"
                     /locus_tag="ACL297_000686"
     CDS             23888..24586
                     /gene="dapD"
                     /locus_tag="ACL297_000686"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGR0070983.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            24654..25784
                     /locus_tag="ACL297_000687"
     CDS             24654..25784
                     /locus_tag="ACL297_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGR0070984.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            25796..26335
                     /locus_tag="ACL297_000688"
     CDS             25796..26335
                     /locus_tag="ACL297_000688"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGR0070985.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            26319..26996
                     /locus_tag="ACL297_000689"
     CDS             26319..26996
                     /locus_tag="ACL297_000689"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGR0070986.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(27068..27967)
                     /gene="galU"
                     /locus_tag="ACL297_000690"
     CDS             complement(27068..27967)
                     /gene="galU"
                     /locus_tag="ACL297_000690"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGR0070987.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDEATGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMNDYEKTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(27989..29005)
                     /locus_tag="ACL297_000691"
     CDS             complement(27989..29005)
                     /locus_tag="ACL297_000691"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGR0070988.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVAVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            29163..29657
                     /locus_tag="ACL297_000692"
     CDS             29163..29657
                     /locus_tag="ACL297_000692"
                     /inference="COORDINATES: protein motif:HMM:NF037495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0070989.1"
                     /translation="MIQYMLIIEVNNSGSSCRLREFGEKIKRLRLAKKISRSEFCGDE
                     SELSIRQLIRIENGESRPILTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRT
                     PTYEDETITQKKESVFDKIFEEYYDRLPEEERFIIPNYSYLALTNYTVQKLPEKLVEI
                     LSFW"
     gene            complement(29772..30206)
                     /locus_tag="ACL297_000693"
                     /pseudo
     CDS             complement(29772..30206)
                     /locus_tag="ACL297_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(30274..30924)
                     /gene="phoU"
                     /locus_tag="ACL297_000694"
     CDS             complement(30274..30924)
                     /gene="phoU"
                     /locus_tag="ACL297_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGR0070990.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(30939..31691)
                     /gene="pstB"
                     /locus_tag="ACL297_000695"
     CDS             complement(30939..31691)
                     /gene="pstB"
                     /locus_tag="ACL297_000695"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGR0070991.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(31693..32508)
                     /gene="pstA"
                     /locus_tag="ACL297_000696"
     CDS             complement(31693..32508)
                     /gene="pstA"
                     /locus_tag="ACL297_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGR0070992.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(32501..33364)
                     /gene="pstC"
                     /locus_tag="ACL297_000697"
     CDS             complement(32501..33364)
                     /gene="pstC"
                     /locus_tag="ACL297_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGR0070993.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(33482..34357)
                     /locus_tag="ACL297_000698"
     CDS             complement(33482..34357)
                     /locus_tag="ACL297_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGR0070994.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(34524..35855)
                     /gene="pnpS"
                     /locus_tag="ACL297_000699"
     CDS             complement(34524..35855)
                     /gene="pnpS"
                     /locus_tag="ACL297_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HGR0070995.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(35856..36563)
                     /locus_tag="ACL297_000700"
     CDS             complement(35856..36563)
                     /locus_tag="ACL297_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGR0070996.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(36647..37132)
                     /locus_tag="ACL297_000701"
     CDS             complement(36647..37132)
                     /locus_tag="ACL297_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGR0070997.1"
                     /translation="MAPTDKEDLIRTLELAPHIEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVVPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            37118..37675
                     /locus_tag="ACL297_000702"
                     /pseudo
     CDS             37118..37675
                     /locus_tag="ACL297_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(37990..39681)
                     /gene="argS"
                     /locus_tag="ACL297_000703"
     CDS             complement(37990..39681)
                     /gene="argS"
                     /locus_tag="ACL297_000703"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGR0070998.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            39817..40263
                     /gene="argR"
                     /locus_tag="ACL297_000704"
     CDS             39817..40263
                     /gene="argR"
                     /locus_tag="ACL297_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGR0070999.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            40314..42848
                     /gene="mutS"
                     /locus_tag="ACL297_000705"
     CDS             40314..42848
                     /gene="mutS"
                     /locus_tag="ACL297_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGR0071000.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     VSSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            42995..44689
                     /gene="patA"
                     /locus_tag="ACL297_000706"
     CDS             42995..44689
                     /gene="patA"
                     /locus_tag="ACL297_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HGR0071001.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT        13013 a         8544 c         9768 g        13449 t
ORIGIN      
        1 aaccatacct acggtaaggc gacgctgacg tggtttgaag agattttcga agagtattag
       61 cgctaatttt tagggctttc acctgacttt taaagactgg caaacttttc tgatgatttt
      121 catgaagagc ctgcgctttt atggtaaaat agtaacagaa taaaagagga gagaaataat
      181 gaaacgtagt atgtatgctg gtcgtgttcg tgaggaacac atcggacaag aaataacctt
      241 gaaaggatgg gttggccgtc gtcgtgacct tggtggtttg atctttatcg atcttcgtga
      301 ccgtgaagga atcatgcagt tggttatcaa ccctgaaaaa gtatctgcag aggttatggc
      361 aacagctgaa agccttcgta gcgaatttgt tattgaggtg actggtcagg tcgctgcgcg
      421 tgagcaagcc aatgataagt tgccaactgg tgcggttgag ttaaacgtga cagctcttat
      481 tgtgcttaac acagctaaga caacaccatt tgagattaag gatggcattg aggcaaatga
      541 cgatacacgt ttgcgttacc gttaccttga ccttcgtcgt ccagaaatgt tggaaaatct
      601 taaacttcgt gccaaggtga cccactctat ccgcaactac ttggatgagt tggagtttat
      661 cgacgtggag acaccattcc tttctaagtc aacgcctgaa ggggcgcgtg attatttagt
      721 gccgtctcgt gttaataagg ggcattttta cgctcttcct caaagtccac aaatcacgaa
      781 acagctcttg atgaatgctg gttttgaccg ttactaccaa atcgttaaat gtttccgtga
      841 cgaggacttg cgtggagacc gccagcctga atttactcag gtcgacttgg aaacgtcctt
      901 ccttactgag caagaaatcc aagatattac agaaggcttg atcgcgcgcg tgatgaagga
      961 aacaaaaggc atcgaagtaa cgctaccgtt ccctcgtatg aaatacgatg atgctatggc
     1021 tctttacggt tctgacaagc cagatactcg ttttgacatg ttgcttcagg acttgacaga
     1081 agtggtcaaa ggtgtagact ttaaagtctt ttcagaagca cctgctgtaa aagcgattgt
     1141 ggtcaaagga gctgcggaca actattcacg taaagacatc gacaagatga cggaagtagc
     1201 caaacagtat ggtgccaaag gtcttgcttg ggtcaaggtg gttgatggag aattaaacgg
     1261 accagttgcc aagttcttga ctggtatcca agaagaattg acaacagcgc ttgctcttga
     1321 agataaggac ttggttctct ttgtggcgga tacgcttgaa gtggctaatg caacactggg
     1381 tgcccttcgt ggacgtattg ctaaagagct tggcttgatt gataatgata agttcaactt
     1441 cctttgggtg gttgactggc cgatgtttga atggtctgaa gaagaaggcc gctacatgag
     1501 cgcccaccat ccattcacac ttccacagga agagactgct cacgaattag aaggtgattt
     1561 ggctaaggtt cgtgccattg cttacgatat cgtcttgaac ggttatgagc ttggtggtgg
     1621 tagccttcgt atcaaccaaa aagaccttca agaacgcatg ttcaaggctc ttggtttctc
     1681 aactgaagaa gccaatgacc agtttggttt ccttcttgaa gccatggact atggtttccc
     1741 accacacggt ggtttggcta tcgggcttga ccgttttgtc atgttgcttg ctggagaaga
     1801 aaatatccgt gaagtcattg cctttcctaa gaacaacaag gcaactgacc caatgacaca
     1861 agctccatca acagtcgctc tcaaacaact agaggaactc agcttacaag tagaagaaga
     1921 tgaaacaaac aaaacgaatt aagcggtggc gctattatct gcgccgcttt gcttatcaga
     1981 taaaaatttt acgtgtctta caaagtatct ctcgagaaaa gtatgatgag aagatttcgg
     2041 cctctctggt ctatggtttt ttatcagcag tagcagttaa tttctttttc caatcagggc
     2101 atgtgtattc gagtggtgca acaggtctgg cacagattat ctctgccttg agtaatcact
     2161 ggtttggttt tcatattccg atttcgctaa gcttctacgc cattaacttc cctttgatgg
     2221 tcttagcttg gtatcagatt ggccataagt tcaccgtctt tacctttatc acggtatcta
     2281 tgagttcctt ctttatccag tttgtccctg tggcaacctt gacggaggat cccattatca
     2341 attccctttt tgggggtgtt gttatgggtt tggggattgg ttttgctctt cgaaacaata
     2401 tctccagtgg tgggacggat atcgtcagcc tgactattcg caagaaaacg ggtaagaatg
     2461 tcggtagtat ttctttcttg gtaaatggaa ctatcatgct gatagcaggt ttgacctttg
     2521 gttggaaata cgctctttat tctatgatta ccatctttgt ctctagccgt gtgacagacg
     2581 cagtctttac taagcaaaag cgtatgcagg ccatgattgt gacaaatcat ccagagaagg
     2641 taattgaaaa aatccataaa aaattgcacc gcggagcaac catgatccac gatgcagaag
     2701 gaacctataa tcacgagaga aaggcagttt taatcactgt cattacacgt gcagagttta
     2761 atgaatttaa acagattatg acacaagtgg atccaagctc ctttgtctct gtctcggaaa
     2821 atgttcatat tctaggaaga tttgttgaga tagataatta gtgattaata ctcttcgaaa
     2881 atctcttcaa accacgtcag cttcaccttg ccgtatatat gttactgact tcgtcagttc
     2941 tatctgcaac ctcaaaacca tgttttgagc tgacttcgtc agttctatct gcaacctcaa
     3001 aaccatgttt tgagctgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc
     3061 aacctgcggc tagcttccta gtttgcactt tgattttcat tgagtataaa aagatcttgg
     3121 gaaaagactc aatttcagta gaaaaggaag taaatcttcc cacaataaaa cgcatagtat
     3181 caagtttttt caatacctga tagtatgcgt ttttgctttt aacgactctt tgcccagtct
     3241 cttatttttc gataattttg tgggcgatca attgacggta acaaatttgt ttattgtttt
     3301 tattatcatt aataatctgg agaatagttt caagggaaac acgaccaagc tctaaactat
     3361 tgatatcgac ataggctgcc aagttgagct tgggattaac cgagtcaaag ctgagaacag
     3421 ggacatccag ctggtgtttg gcaatatagt tacaaacacc ttcagctagg aggctatcgg
     3481 ttgtgatgat agcatcaatt tgtggatcgt gcttgaataa tcgcttgcta aatttatagc
     3541 ccttttcttc cagaaactcg tcggcaaagt agatgcgatt gttgtcagtg gtaagtttgt
     3601 aatgtttaag cgcctgttca tagcctgtta aacggtcttt ggtcacgaag agctttttac
     3661 ttcctccgat aaaggcaatg cgtttgcagc cttttttgat gaaatattca gtcgcatcaa
     3721 aaccagcttg aacattgtcg ttgtcgacaa gtgggatgaa aggagataga gatttaccta
     3781 ggataaggaa ggggaactgt tcttctgcga cgagttttac gagagggtct tcttcttggg
     3841 catagagaaa gattagccca tctacacgct tgccgtagac catttgtgaa atagcgttga
     3901 gacgctcctt ctcatctttc cctgttgcta tctgaatggc atagtggttt tcagatgcga
     3961 cttgagagat gccacgtaga accgatggaa agaaaggatt ctggtagaag gcgtctgagt
     4021 catcaggaag aactaatccg ataacctgag tatagctgct taccaagcta cgagcgttga
     4081 ggtttgggtg gtagttgagt tccttcatag ccttgcgaac gcgttttttt gtttcgtcgc
     4141 taatggttga tttattttga ataacacggg ttacggttga aggcgaaaca ccagcagcct
     4201 tggccacgtc tttaatcgta acgggcataa aaatctccta tttatggtaa tttggatcac
     4261 ggaaatgtgt tttataaaag atagtgacca gatacagaac aaaaagaata ttttgtacag
     4321 taatcagggt tgtcatattt tggccaaata atcccaaaat aagtgtaatc agagtcggca
     4381 atcctaaaca gttcaagata aagtgatagc actctttaaa ggttctaaat gaaaagaggc
     4441 gtgatttctt agtgatataa aggagaagac tagctcctag agagacgata aagaaattca
     4501 aaccaaagag gaagctcgca ccgagaacta ggaagaggct gatatagaca cgattttgtt
     4561 ggtaccagtc tttagaaatt gcttgggtca agctgtcttt gcttttgaaa ctctcagtct
     4621 gaatggctcg gtaagagatg cgggtcagtt ccttgctttc cttgctgatg actagctcat
     4681 ttgtatcgaa atgcagttgc aagttcttag gtaattcctt gatttgactt ggaccaatca
     4741 caacagaaga ggcttgacta actgttccag tataagttaa tgtgccatcg acaattgtag
     4801 catgttcaga gagatcctgg acaacctcat ttgtcagagg ttcatagaca ttatcgataa
     4861 aagtttctag cggataggtc tcctgggagc tgttttggat agcaatgggt accatagaca
     4921 agctgataag gaagatactg gtaaagagaa gttgaaacca gttgagcccg aaacgtttgg
     4981 acaggggctt acgaaacccc aaaatacttg aaaaaaagga aaatggatat ggaagcatag
     5041 gtatctttct aaaaggtgtt ttctatataa gtattattat atagagaaat gcgctttatt
     5101 tcaagtctag gagaaaaatt gcttgctaca aggatattat ttacagtgac aattgctaat
     5161 aatcaatgaa aatcaaagtg caaactagcc acaggttgct caaagcacaa ctttgaggtt
     5221 ggagataaag ctgacgtggt ttgaagagat tttcgaagag tataaaatga aaaagggtgg
     5281 cggggataaa ttatcccttg tcgccaccac ttgtaagtcc tgaaacaaag ttcttttgta
     5341 ggaagaagaa gagaatacag attggaaggg cgatgaggat agcacctgct gagaagtagg
     5401 caatcttcaa gtttttcgca ttgttaacga aggtttggag acctacggca acagtaaagt
     5461 attctttctc acgaagcaag aaactagaga ggatgtagtc cccgaaaggt cccatgaagg
     5521 cccagagagc ttgtacggca accattgggc gaacaagtgg aagaacaatt tgccagaagc
     5581 ggcggaagtg tcctgcaccg tctagttttg cagattcgtc taaagacatt ggcactgtat
     5641 cgaagtagcc tttcatgagc caagcattca tcgggatacc accaccaacg tagaggaaga
     5701 tgaggaacca gttgtggtta agggcgttca acataagcgc cataacgaag aaggctgtca
     5761 aagcggccat agttggcacc atttggatga tcaagaagaa gaccaaactt tgtttacgag
     5821 ccaagaagtt gtaacggctg taagcataac cagcaagtac gatgatactt gtttgaacag
     5881 ccatggtaat taaggcgata atcaaagtgt tgaggtacca agtaccgtac aaggtttcag
     5941 tgaagaggcc tttaaagtta tcaaaattga ggtcgatatt agtatctagt ttaaaggctg
     6001 agacgttacc tgctttaaag gctgacataa tggtaatcaa cagtggatag ataattacaa
     6061 ttgatagacc aatcaggtaa aggtaagtaa ggctttgagt cagtctacgt ttgagtttaa
     6121 ttgagttatt catcttagac gtcctccata tcaaatgcgt gtagtttctt gaatgcgatc
     6181 atagagattg agatgacaat gatagagata atcaaggtaa cagctgccgc cattgagtat
     6241 tgaggagatg tacctgttgt caaacggtag atccatgaga tcaagatatc ggttgaacca
     6301 gctccacctc cgacactacc aggtcctcca ccattgaaga ggtacatgat agagaagttg
     6361 ttaaagttga aggtgtattg gctaatcaaa gtaggtgccg caacagccaa aatcattggg
     6421 aaagtgatgt tgcggaattt ttgccaagcg ttggcaccgt caatataagc tgcttcgtaa
     6481 aggtcgttag gaatagattg caagataccc aaggtcagaa cgtagatgta tgggaatccg
     6541 agccaacctt gcatcataat caaggcaatc ttagtccaag ttgggtctgt tttccaagga
     6601 ataagagctc catcaaggaa aggaaggaat ttagccaaga ttggcaatac ttgagtgttg
     6661 atagcaccga cactatcgtt aaacatgttt gagaatgtca agatagtgat gaaggctggg
     6721 acagcccaag gaagaaggaa aataacacca aagatacgtt ttcctttgat aaatggttgg
     6781 ttggcaatga tagctgtgaa gataccaatt acgatttgta aagtagaagc tgccaaagcc
     6841 caaatgatag tccaagaaag aacagaacca aaggcagaac ggaaggtact caagctccaa
     6901 atgtttgtaa agttggtcaa accaacccag tccaacaact tgtttggtgg caagtgttgg
     6961 aagtcgtagt tggtaaaggc gatcatcaag gttacgataa ctgggaagat aatcgcgaag
     7021 gtcatggcaa cataagatgg aatgatcaag aggtaaggga agccattttc ataaatccct
     7081 ttgatcatgt ctttgagtgt gcgtggaact ggaattccat tgttaatgcg ttttgcaatc
     7141 gtatgtgcat ctttgatatt tgagaaataa aagagtacat aaacgattac aaagattaga
     7201 tggaaggcac cacgaatcag cataaagagg gaattatcac gacctggttt gtcaccaaga
     7261 gtgatgaggt tgctcaattc aggggttgca agtgctagga agtaaaggac aaatacgatg
     7321 gttacaccaa ggaagataaa acctttggct ttttgtttat tgtaaatctg tcctaaccca
     7381 ggaatgatag acagcagggc tgctttacta ggttgttgct tttccatatt aactcctttc
     7441 ataggatact acttttttat aagaaagcta aattatttgt gtctctattg ataaattcaa
     7501 agggggattt gatttccacc ccccttgaac aaatttttta ttcaccaaat ttttgtttga
     7561 ttgtttcttt gatcaatgtt acagcatcgt tagcagctgt tttagcatct ttttgaccac
     7621 ttacagcatc aaagagcata tttttcgctg gatcccaaac tgcagacatt tgagagatgt
     7681 ttggcagtgg ttgagtgttc ttgaactgtt tgataacagc tgttgtcaac tcatcgtttt
     7741 taccttcagc gtatgaacga gcctcagtat tagctgggat ttcgttagtc ttatcatata
     7801 atactttttg ttgttcagtt gcaacaagga agtctacaaa tttttgagaa gcttcaaggt
     7861 tcttaacggc ttgaggaatg acccaagctt taccaccacc gaatgcagca tattcttttc
     7921 catttggaag agttgggata gttgcaactc cgtagtttac tttagcatct ttaaaggctt
     7981 gagctttcca aggtccgtcg atgatagcag ctgttttacc ttcttggaat tgagtttgga
     8041 ttaagtttcc agcaccttct gtatcttgca tacctttagg ccatttttcg taccaagatt
     8101 tagcgtagtt gatacctacg atagaaccgt cgtttgcaag accgatgtct ttagcgtctt
     8161 taccgttttg gccaaagacg taagcaccgt taccggcaag aagtccatat gtatagtaga
     8221 agtttgtcca gtcagctagg aaggcagtag ttttaccatc ttcaccagcg aatgcgtatt
     8281 tgctatcttt agcaaggttt tccaagtcag caaatgtttt tggagcatct ttcaccaagt
     8341 ctttgttgta gtacataaca agtgactcga taacggcagg agcaccgtaa actttaccat
     8401 tagcagctgt tacaagagat ttagttgtgt cgtctgtttt agcaccatcg ctcaatttca
     8461 cttctgaaag ttgtccgtca gaaccaaggc tacctacacg gtcgtatgga gccatcataa
     8521 catcagggac attaccagat tggttgtcaa gagaaagttt atcaagacct cctagagcat
     8581 caccagtttt aagagtgact tttactccag cttctttttc ataagcttta gcaacctctt
     8641 caatatagct cttatatccc tcgtctacat atacagtgag ttctttgact tcagatgaac
     8701 cagaatcagc aggcttatca gcagttttgc ttccgcaagc taccaaaagc aagctagcaa
     8761 gtgtaacagt tccaagcacc gcagtgctct tcataaattt agatgacata gtgtattcct
     8821 cccaaagaat agcaagtttt attgataagg aaacgcaaac gttttccttt atgagcttag
     8881 tatagcacaa atagaaaacg gttgcaagta ttttttgtaa aaattttaaa aaatttttaa
     8941 gcttgatttg atagcataat tttgcatttt aatagaagaa aagtatgaaa tagataagaa
     9001 tacagtattt ttaataaaaa tatatggaat ctgtctaaag agggaaacca ggaggggcta
     9061 cctccctggt tctaatccct atctattttc tatggacgtt tgtgctttgt tacatataaa
     9121 ttcaagctcc ctaaggaggt tttgtacttt gtagactgtc tcgtgagagt caaaccttag
     9181 gattagatag ttttaagtta tctttgctaa aatgagttag tctccttaaa aataatcaaa
     9241 aaagtttttt aaaaacgctt gcaattatgc ttgaaaagga gtatacttat aagtaacgca
     9301 aacgtttgcg tctgcaaaat acgcaacgtt ccattatttt aacacacgag gtgctattat
     9361 gaaaaaacgt caaagtggtg tgttgatgca catctcttct cttccaggag cttacggaat
     9421 cggatcattt ggtcaaagtg cttacgactt cgttgatttc ttggtccgta caaaacaacg
     9481 ttactggcaa atccttccat taggagcaac tagttacggg gattctcctt accaatcttt
     9541 ctcagccttc gcaggaaaca ctcattttat cgatttagat atcttggtgg agcaaggttt
     9601 gttggaagca agtgaccttg aaggagttga ctttggtagc gatgcgtctg aagttgacta
     9661 tgctaaaatc tactatgcac gtcgtcctct tttagaaaaa gcggtgaaac gtttctttga
     9721 agtcggagat gttaaagatt ttgagaaatt tgctcaagac aaccaatcat ggcttgagct
     9781 ctttgctgag tatatggcta tcaaagagta ttttgacaat cttgcttgga ctgaatggcc
     9841 agatgcagat gctcgtgctc gtaaagcttc agcacttgaa agctatcgtg agcaattggc
     9901 agacaagttg gtttaccacc gtgtgactca atacttcttc ttccaacaat ggttgaaatt
     9961 gaaagcttac gctaacgaca accacatcga aatcgttggg gacatgccaa tctacgtagc
    10021 ggaagattca agtgatatgt gggcaaatcc acatctcttc aaaacagatg tcaatggtaa
    10081 ggctacttgt atcgcaggat gcccaccaga tgagttttct gtaactggtc agctttgggg
    10141 taacccaatc tatgactggg aagcaatgga caaagacggc tacaaatggt ggattgaacg
    10201 cttgcgtgaa agcttcaaaa tctacgatat cgttcgtatc gaccacttcc gtggcttcga
    10261 atcttactgg gaaatccctg ctggttccga tacagcagca cctggtgagt gggtgaaagg
    10321 tccaggctac aagctttttg cagccgttaa ggaagaactt ggtgagctaa acatcatcgc
    10381 agaagacctt ggcttcatga cagatgaagt gatcgaattg cgtgaacgta ctggcttccc
    10441 aggaatgaag attcttcaat ttgccttcaa cccagaagac gaaagcattg atagcccaca
    10501 cttggcacct gctaactcag ttatgtacac aggaacacac gataacaata cggttcttgg
    10561 ttggtaccgt aatgagattg atgatgcgac tcgtgagtac atggctcgtt acacgaaccg
    10621 taaagaatac gaaacagtgg tacacgctat gcttcgtaca gtattttcat cagttagctt
    10681 tatggcaatt gcaactatgc aagatttact agaattggat gaggcagctc gtatgaactt
    10741 cccatctacc cttggtggaa actggtcttg gcgtatgact gaagatcaat tgacaccagc
    10801 tgtcgaggaa ggtttgcttg acttgacaac aatttatcgc cgaattaatg aaaatttggt
    10861 agatttaaag aaataagaca ataatcagga gacaactaaa catgttatca ctacaagaat
    10921 ttgtacaaaa tcgttacaat aaaaccattg cagaatgtag caatgaagag ctttaccttg
    10981 ctcttcttaa ctacagcaag cttgcaagca gccaaaaacc agtcaacact ggtaagaaaa
    11041 aagtttacta catctcagct gagttcttga ttggtaaact cttgtcaaac aacttgatta
    11101 accttggtct ttacgacgat gttaaaaaag aacttgcagc tgcaggtaaa gacttgatcg
    11161 aagttgaaga agttgaattg gaaccatctc ttggtaatgg tggtttggga cgtttggctg
    11221 cctgctttat cgactcaatt gctactcttg gtttgaatgg tgacggtgtt ggtcttaact
    11281 accactttgg tcttttccaa caagttctta aaaacaacca acaagaaaca attccaaatg
    11341 catggttgac agagcaaaac tggttggttc gctcaagccg tagctaccaa gtaccatttg
    11401 cagactttac tttgacatca actctttacg atattgatgt tactggttat gaaacagcga
    11461 ctaaaaaccg cttgcgtttg tttgacttgg attcagttga ttcttctatt attaaagatg
    11521 gtatcaactt tgacaagaca gatatcgctc gcaacttaac tctcttcctt tacccagatg
    11581 atagtgaccg tcaaggtgaa ttgctccgta tcttccaaca atacttcatg gtttcaaacg
    11641 gtgcgcaatt gatcatcgac gaagcaatcg aaaaaggaag caacttgcat gaccttgctg
    11701 actacgcagt tgtccaaatc aacgatactc acccatcaat ggtgattcct gaattgattc
    11761 gtcttttgac tgcacgtggt atcgatcttg acgaagcaat ctcaattgtt cgtagcatga
    11821 ctgcctacac taaccacaca atccttgctg aagcgcttga aaaatggcct cttgaattct
    11881 tgcaagaagt ggttcctcac ttggtaccaa tcatcgaaga attggaccgt cgtgtgaagg
    11941 cagagtacaa agatccagct gttcaaatca tcgatgagag cggacgtgtt cacatggctc
    12001 acatggatat ccactacgga tacagtgtta acggggttgc agcactccat actgaaatct
    12061 tgaaaaattc tgagttgaaa gccttctacg acctttaccc agaaaagttc aacaacaaaa
    12121 caaacggtat cactttccgt cgttggctta tgcatgctaa cccaagattg tctcactact
    12181 tggatgagat tcttggagat ggttggcacc atgaagcaga tgagcttgaa aaactgttgt
    12241 cttatgaaga caaagcagct gtcaaagaaa aattggaaag catcaaggct cacaacaaac
    12301 gtaaattggc tcgtcacttg aaagaacacc aaggtgtgga aatcaatcca aattctatct
    12361 ttgatatcca aatcaaacgt cttcacgagt acaaacgcca acaaatgaac gctttgtacg
    12421 tgatccacaa ataccttgac atcaaagctg gtaacatccc tgctcgtcca atcacaatct
    12481 tctttggtgg taaagcagct ccagcctaca caatcgctca agacattatc catttaatcc
    12541 tttgcatgtc agaagttatt gctaacgatc cagcagtagc tccacacttg caagtagtta
    12601 tggttgaaaa ctacaacgtt actgcagcaa gtttccttat cccagcatgt gatatctcag
    12661 aacaaatttc acttgcttct aaagaagctt caggtactgg taacatgaaa ttcatgttga
    12721 acggagcttt gacacttggt actatggacg gtgctaacgt ggaaatcgct gagttggttg
    12781 gagaagaaaa catctacatc ttcggtgaag attcagaaac tgttatcgac ctttacgcaa
    12841 aagcagctta caaatcaagc gaattctacg ctcgtgaagc tatcaaacca ttggttgact
    12901 tcatcgttag tgatgcagtt cttgcagctg gaaacaaaga gcgcttggaa cgtctttaca
    12961 atgaattgat caacaaagac tggttcatga ctcttcttga cttggaagac tacatcaaag
    13021 tcaaagagca aatgcttgct gactacgaag accgtgacgc atggttggat aaagtcatcg
    13081 ttaacatttc taaagcagga ttcttctcat ctgaccgtac aatcgctcag tataatgaag
    13141 acatctggca cttgaactaa tactcttcga aaatctcttc aaaccacgtc agctttatct
    13201 gcaacctcaa agcggtgctt tgagcaacct gcggctagct tcctagtttg ctctttgatt
    13261 ttcattgagt ataagataca aatttatact aatacatttt gtaaaaaagc gagtttcgat
    13321 tgaaattcgc ttttttaaat gctgtggatt tgggtcaatc ttgtctaaaa atagggaaat
    13381 cctagataca gtgaaggctt taaatgctgg tttttactgt cttcagcctt atattttttc
    13441 gtagttggtt acctcatatc tcttgtattc gcttacataa agtattataa tataattgta
    13501 ggaaagaagg tgtttttatg atatacacac ttaaattggt gttgtttatt acctttcttg
    13561 taataagctt gttacctgat aagatttttg gaaaaaataa aaaaatttgg aaaatagttt
    13621 ttgcaatatt gacggcagtg gcagcattgt catttatgta ctaagttatt ttaagaatgt
    13681 agggaaataa accctacatt tttttagttt tttctgtttt ctaaattcta tttatccaag
    13741 agcttcaaca tttcttgctt cttcgcttca agttctgcac gcttttcttc gatttcggca
    13801 tgttttttct cgagttcaga acaacttgca tcattgctaa attcttttcg ccatcaggag
    13861 atagggtgag tctacatgtc tattactcac ccaaagcagt cctacaaagc aggaattttc
    13921 tgttactttt ttggaaatag taacgtttat acagctttga cacttcgtat caaagcgcca
    13981 aacacactcc gagggattta cagaaagcag aaaaggaatg atctggtata agatcattcc
    14041 ttttctctct ttttctttaa gtaattatat acaatgtacg acgaagtcgt cattgcaatg
    14101 ctgatccacc acctaaaggg aactttaaac aacattgata agataaagaa tataaacaac
    14161 gaaaatacgt tatacccaat taattttatt gtatatctca tgattaaaag ttaatccttc
    14221 cgttgttagg aatggcatca tttttatccc ataattgtgc taaataagtc cccggtgata
    14281 ataaattcat agcgaattct aaagcaacat catttacaaa ccaactacct agatatctag
    14341 aaattgctga acgaatagca ctttttgctg catgttttcc ttttacttta attagatttg
    14401 caaggcctgc agtagttcct cctaatgcta aagctattgc agtatctaat agagcaccca
    14461 tttgattaac tgtaatacct tgccaagctg ctctaaatgg agagtatgta ggtgggattg
    14521 tataatcgcc ttgtaattgt cggttaatta cttctttgat ccattgttgt gagacgtctg
    14581 gatgaaaaga ttggatttcg tttgcaagtg tattgatttg ttcttctgtt agagaagtga
    14641 caggttgaag ttccatattt gtttcaattt gtgatacttg ttcagaagcg tatacagctg
    14701 aaacacttgg aatcgctgat acaattaaca caattgacgt caaaaaaacc gaaataaatt
    14761 tcattaattt gttcatgagc ttttctcctt tttatttgta tctgcttaca ttttatcata
    14821 tactgttatt atagtcaaaa aaatatgcta ttatgttaaa aaaatatttt tcaaaatata
    14881 aatggacgga tttattttgg attttatttg ttattttgac ctgcctctat attggtaacc
    14941 atgatttgtt tactctcaat catcaagaat tctcttttcg tggtagcgtt tggggtctgg
    15001 tactggcctt atatcactta ttgttcattg ataagtttgt aatttcgaat cgaaaataaa
    15061 gattagaact atgattgact ggctactttt aatattgatt ttggaggaag attttgtctc
    15121 tattatttat tattttaaat ttatctattt tgtacaagat ttattctttg agacgggaga
    15181 aatcgaaata cttgatttat acggcctata tcatatttgg ggtaaatgta gtatatggta
    15241 ttcaatggtt attaagagaa ctgatttcaa ctatttcccc ttaatgtgta tggaaactat
    15301 aacaaaacgc ataagattaa ggtttgtgta cctgttctta tgcgttttta taatattgaa
    15361 gattttcggt aagttttata cctttgggaa gtctagttta gaatgctttc ccaaccaaaa
    15421 tctctgcttc gatggtaatc tctgtcagtt ggctccagtc aatcttgctc tggtcaacgt
    15481 gaaagaggag aggagtcata ctgagtaggg cttggagttg ttctgctgtg atagtcttag
    15541 tcagagaggc agtttgacta gataggatgg taaagtgttc ctggaaatgc tccttgatat
    15601 cttggttaga atactccttg tttgtcagct ggtcctgtac tctttgacgg atttctttga
    15661 ggtgattttc agttgggata acctttatca agataccgtc tttggataaa acgcgacgaa
    15721 attctccata gttggcaggt gagaagatat caagcagaat atccatgtta gcgtctttta
    15781 taggaagtcg tgccaagtcg ccaacaaacc aattgactgc ccagttgggt tcacttttag
    15841 cagcgatttg gactgaatct ttggagatgt caaaggcata gaaagttttt tcagagtgac
    15901 tttcttgtag tttgcgagaa tagaatcctt caccacaacc gatatccaaa attgttgtgg
    15961 tagtttttga gcttgcaagc aagtcagata cagcatctaa gatagcttgg taaaagccgg
    16021 cttctaggat ttgttgacgg ttttgaaaat tttccttgtt atagttagca gattgcttga
    16081 tttgaggtgc tagattgaca tagccaaatt tcgccaagtc aaaagaatga cggttgcagc
    16141 acttgaaatt agtctctaac agagtcagat tttcttgaca gataggacag gcaaaggcag
    16201 tcgcagaagc aaaacgctga agtttgggct tgagatttgt attcatagtt taagtgtatc
    16261 aaaagaggca aatgaaagca actttaggaa taagtagatg ggagattcag tagaaataaa
    16321 actaattctc caatttatag aatgaaattg cttttatatc taaattgcta tataataatg
    16381 ataaggagga aataagtatg ttaaaagaag tattaaccgt tgcaaaagtt gcgaaaaaat
    16441 cttcattatt tttgggtggt gtcgcatttg gtacccttgg tttgaaaatc ttagcaagta
    16501 aggaagctaa aaaaggttat tctaaagctt tggctaaggc ttacaagttg aaagacgagc
    16561 tagatgcatc tgtttctgtt gtgaagcaac atggagacga tgtcttgcaa gatgccaaat
    16621 atttgtacga gcaagagaaa aaagaagagc aattagatag ccttataggt gaataatatg
    16681 tcttttaaag tgctacatag aggataccaa catatccgac tatcatcttc tttttcactt
    16741 accttggata ttcaagacta tcttcgttcc ttggcgagag atgaaaaggg gattgagtct
    16801 atccagtttt acatggatca acagcacttt actctacgca taaaagaagg cttttctgta
    16861 ttagataatg cagaagcctt tttaaaaaga attgataaag ggaaagtttc tgagttgatg
    16921 actcttccca ttcgtagaga agagagtgct tattctattg tttcaggtgc agcggttaag
    16981 cgtgtacttt ttcgtagttt tgtgccgtat cctattcgct atatatggac ttgttatcag
    17041 gctttgggtt atattagaga agcctatcaa acactagcgc gtaaggaact aacgatggaa
    17101 gtcttggact gttcggctat tttattgtct ttgtttatga accaatccaa gacagctagc
    17161 aatatcatgt ttatgcttga tttggggaat catttagatc agtggtcctt gaaaaaaact
    17221 gcaacagatt tagaacagag tcttcttgca aaagagagcg atgtattcct agtacagggc
    17281 gatacggttg ttagtatcaa gagttccgat gttcaaatag gagatgtctt gatcttatct
    17341 caaggaaatg aaattctgtt tgatggacaa gtagtttcag gtttaggtat ggtcaacgaa
    17401 agttccttga caggagagag ttttccagtt gaaaaaagag agtctgattt ggtttgtgca
    17461 aatacagtat tagaaactgg agagttacgc attcgtgtaa cagataatca gatgaacagc
    17521 cgtattttac agctgattga gttgatgaag aaatctgaag aaaacaagaa aacgaaacaa
    17581 cgctatttca tcaagatggc ggataaagtc gtcaaatata atttcttggg ggctgggctg
    17641 acttacctat tgacaggttc tttttctaag gctatttctt tcctattggt cgatttctcc
    17701 tgcgctttga aaatctctac tcctgtagct tatttgacag ttatcaagga agggttgaac
    17761 cgtgaaatgg tgattaagga tggagatgtt ctggagaaat atctggaagt tgatactttc
    17821 ttgtttgata agacaggaac aatcacaact agttatccta tagttgaaaa ggtgttacct
    17881 tttggagact atagtgagga agatattctc agaatcagtg cctgtcttga ggaacacatt
    17941 tatcatccta ttgctaatgc catcgtcaag caagctgaga tagagggaat tgaacatgag
    18001 gaaatgcatg ggaaactcca atatatcgca agcaagggga tcaaatctca tatagatggc
    18061 caaccagttc ttattgggaa ttatgtcttg atgcaggatg agcagattca tatcagttca
    18121 gaacaaaatg ctttaattga agagtacaag agtcactaca atctcttatt cttggcttat
    18181 caaaatgaat tgattggaat gttctgcatt catactcctt tgagaaaaga agcaaaaaca
    18241 gccttggata aacttaaggc acaggggaaa aaattgattc tggcaacagg ggacaccttg
    18301 gttaggacaa aggaattagt caaagatttg ccctttgatc aggtctatac agacttgaaa
    18361 cctgatggga aatttgagtt agtagagaaa ctgcagaaag caggtcacac tattttgatg
    18421 gttggagatg gattgaatga ctcagcggct ttaactctat cagatatcgg tgtggtgatg
    18481 aatgagagtg cagatatttc taagcagatg agtgatatct tattgttaga taatcgtttg
    18541 gatttcttcc aagagttgga ttcgttatca tcatctttgc aaacactcat caagaaaaat
    18601 attcaagata ccgttgtcgt aaatagtagt ttgattggct tcggcttatt taattggctc
    18661 agtccttcaa atctctctat cttacataat ctaacaacct tgcgcattgt cctgcgtagc
    18721 ctgtctatta aaaatagata gatgagagtt attaacagca aaaaggagtt acctttctcg
    18781 aggttaactc ctttgtttat agataaatgt tgaatagttt agtaagtcaa tacaaagtat
    18841 tttttcttcc cacgacggat aacagtcagt tcattctcta acttatcagc gtcactcaag
    18901 acatagtcaa gctcttggat gcggtcgccg tttacgtaga tggctccgtt ttggacgtct
    18961 tcacgggctt ggcgttttga gttaactata ccagatgaga cgagcagttc cacgatattg
    19021 ttgttttcgt ctgcctgtac ttggtagttt ggcacaccac gaagtccttg tttgagctct
    19081 ttgacagaaa ggtttttgat gtttcctgca aagagttgct cagtgatgtt aagtgcttct
    19141 ttgtaggctt cttctccgtg aacaagtgta acaacttcac gagccaagac tttttgagcc
    19201 aagcgttcgt gtggcgctgc ttcaaattgt ttacgaatat cttcaatctc atcaagtgac
    19261 aagaaagtaa agattttcaa gaagcgaaca gcgtcagcgt ccatcacgtt catccagaat
    19321 tggtacattt cgtatggaga agtcttttcg ggattgagcc agacggcatt tccttctgat
    19381 ttaccaaatt tcttaccagt tgcatctgtg attagtggaa cagtgataac gtgaccagtc
    19441 ttgtccgcct tacgacgaag caattcggta ccagctgtca tatttcccca ctggtcagaa
    19501 ccaccgattt gaagagtgac attatggtct tggttaagga cgaagaagtc atacccttgc
    19561 atgatttggt aagcgaactc agtgtaagaa attcctgttt cgatccgttt tttaacagat
    19621 tccttactca tcatgtagtt gaccgtgaag tattttccaa tatcacggag gaagtcaatg
    19681 aagctgatgc tgccaaacca gtcgtagttg ttgaccatga cagccttgtt ttcgccattt
    19741 tcaaagtcaa gaaaacgaga aagttgtcct tggatagact tgacccagcc atctactgtg
    19801 tcttttgttt ggagactacg ttcagcatct ttgaaggacg gatctccgat gagacctgta
    19861 gcaccgccaa cgagcgcata aggtttgtga cctgctagtt gcaagcgacg acttgtcaag
    19921 attgcgacaa ggtggcctag gtgaaggctg tcagcagttg gatcgtagcc agtataataa
    19981 gaaacttgac cttcttctag ggctttacgc aaagcttctt catcagtcgt ttgaaatatc
    20041 aaaccacgct cttttagctc atcaaaaatg tgcatgtgtt ttttctcctt tttaaaatta
    20101 ttgtttctac ctattgtatc acaaacttgg gcaatagcct agcagaatag ggaagaaagt
    20161 ggctatttta ttgaaaattt cccttttgtg gtataataga taaagtgagg aaatccatgc
    20221 aaaatcaatt aaatgaatta aaacgaaaaa tgctggaatt tttccagcaa aaacaaaaaa
    20281 ataaaaaatc agctagacct ggcaagaaag gttcaagtac caaaaaatct aaaaccttag
    20341 ataagtcagc cattttccca gctattttac tgagtataaa agccttattt aacttactct
    20401 ttgtactcgg ttttctagga ggaatgttgg gagctgggat tgctttgggg tacggagtgg
    20461 ccttatttga caaggttcgg gtgcctcaga cagaagaatt ggtgaatcag gtcaaggaca
    20521 tctcttctat ttcagagatt acctattcgg acgggacggt gattgcttcc atagagagtg
    20581 atttgttgcg cacttctatc tcatctgagc aaatttcgga aaatctgaag aaggctatca
    20641 ttgcgacaga agatgaacac tttaaagaac ataagggtgt agtacccaag gcggtgattc
    20701 gtgcgacctt ggggaaattt gtaggtttgg gttcctctag tgggggttca accttgaccc
    20761 agcaactaat taaacagcag gtggttgggg atgcgccgac cttggctcgt aaggcggcag
    20821 agattgtgga tgctcttgcc ttggaacgcg ccatgaataa agatgagatt ttaacgacct
    20881 atctcaatgt ggctcccttt ggccgaaata ataagggaca gaatattgca ggggctcggc
    20941 aagcagctga gggaattttc ggtgtagatg ccagtcagtt gactgttcct caagcagcat
    21001 ttttagcagg acttccacag agtcccatta cttactctcc ttatgaaaat actggggagt
    21061 tgaagagtga tgaagaccta gaaattggct taagacgggc taaggcagtt ctttacagta
    21121 tgtatcgtac aggtgcatta agcaaagacg agtattctca gtacaaggat tatgacctta
    21181 aacaggactt tttaccatcg ggcacggtta caggaatttc acgagactat ttatacttta
    21241 caactttggc agaagctcaa gaacgtatgt atgactatct agctcagaga gacaatgtct
    21301 ccgctaagga gttgaaaaat gaggcaactc agaagtttta tcgagatttg gcagccaagg
    21361 aaattgaaaa tggtggttat aagattacta ctaccataga tcagaaaatt cattctgcca
    21421 tgcaaagtgc ggttgctgat tatggctatc ttttagacga tggaacaggt cgtgtagaag
    21481 tagggaatgt cttgatggac aaccaaacag gtgctattct aggctttgta ggtggtcgta
    21541 attatcaaga aaatcaaaat aatcatgcct ttgataccaa acgttcgcca gcttctacta
    21601 ccaagccctt gctggcctac ggtattgcta ttgaccaggg cttgatggga agtgaaacga
    21661 ttctatctaa ctatccaaca aactttgcta atggcaatcc gattatgtat gctaatagca
    21721 agggaacagg aatgatgacc ttgggagaag ctctgaacta ttcatggaat atccctgctt
    21781 actggaccta tcgtatgctc cgtgaaaagg gtgttgatgt caagggttat atggaaaaga
    21841 tgggttacga gattcctgag tacggtattg agagcttgcc aatgggtggt ggtattgaag
    21901 tcacagttgc ccagcatacc aatggctatc agaccttagc taataatgga gtttatcatc
    21961 agaagcatgt gatttcaaag attgaagcag cagatggtag agtggtgtat gagtatcagg
    22021 ataaaccggt tcaagtctat tcaaaagcta ctgcgacgat tatgcaggga ttgctacgag
    22081 aagttctatc ctctcgtgtg acaacaacct tcaagtctaa cctgacttct ttaaatccta
    22141 ctctggctaa tgcagattgg attgggaaga ctggtacaac caaccaagac gaaaatatgt
    22201 ggctcatgct ttcgacacct agattaaccc taggtggctg gattgggcat gatgataatc
    22261 attcattgtc acgtagagca ggttattcta ataactctaa ttacatggct catctggtaa
    22321 atgcgattca gcaagcttcc ccaagcattt gggggaacga gcgctttgct ttagatccta
    22381 gtgtagtgaa atcggaagtc ttgaaatcaa caggtcaaaa accagggaag gtttctgttg
    22441 aaggaaaaga ggtagaagtc acaggttcga ctgttaccag ctattgggct aataagtcag
    22501 gagcgccagc gacaagttat cgctttgcta ttggcggaag tgatgcggat tatcagaatg
    22561 cttggtctag tattgtgggg agtctaccaa ctccatccag ctccagcagt tcaagtagta
    22621 gttctagcga tagcagtaac tcaagtacta cacgaccttc ttcttcaagg gcgagacgat
    22681 aattctctaa atgaagtggc caatcaatgg attgccactt ttttcttttc gtgttacaat
    22741 aagggtatga atcagtatca gaaaaagatt gttaacggaa aaatttattc gctcctatcc
    22801 ggcttaatat ggggaatctg tggaatttta ggagagtact tctttactca ttatcaggtg
    22861 tcttcgggct ggattacctc tatgcgtttg acactggcag ggagtcttgt actcatttgg
    22921 tctgcaatac aattaaaatc gcaagtgcta gatatttggc gagacaagaa aaattacctg
    22981 ccctttttag cctatgctat tttggggatt ttttcagttc agtatttttt ctatctctgt
    23041 gtagaatact caaatgctac gacagcaact attttacagt ttattagccc tgtctttatc
    23101 ctcttttaca atcgcttggt ttatcaaaaa cgagcgtcaa aaagcgctgt tttctatgtt
    23161 ttggttgcca tgctgggtgt ttgcttgatg gcgacaaagg gagacctctc tcagttatcc
    23221 atgacgccgc tagctcttat aacaggtttg ctgagtgcca tgggtgttat gtttaatgtt
    23281 atcttgcccc aaccttttgc taagcgttat ggttttgttc ctacggttgg gtgggggatg
    23341 attttggcag gtttgtttag caatgtcctc tcgccggttt atcagctttc ctttactctt
    23401 gatatttgga gtatcttgat ttgcctcatt atcgctttct ttggaacggc ttttgctttt
    23461 ttcatttcca tgaaggctgt gtccttggtt tctcctttgg tggttgccgt tatcagtgcc
    23521 agtgaacctc tctcttctgc tctcttgagt gttttgttct taggattagt agtggattgg
    23581 tccctccttc tagctatagc cttgattatt ttacccatga tatttttgtc tatagaagaa
    23641 gcgaaagaaa gtagataaaa agtcttttct taattctaaa agtgtgctat actagaatag
    23701 tcaataaaac acggggagat agttgtgaag agtgttttta ggttgtatcg gtaggggctt
    23761 gcttttaccg acagcacgtt ttatctcaca tccctaaaaa atcataccta aaaacagaca
    23821 aaaaggtttc aaatttgagg ccttttttgg tagaataggt atcattataa cgaaccagga
    23881 ggcacctatg actgctacaa aaatgaacgc tcaagaaatt atccaattta tcgccaatgc
    23941 tgaaaagaaa accagtgtca aagtaacctt tgaggggcaa ctcgcaactg ctgtgcctag
    24001 ctctgttgtc aaactaggaa atgtcctatt cggagactgg aaggacgtgg ctccgcttct
    24061 tgaaggtttg gtagaaaatc aagattatgt tgtcgagcaa gatgctcgta attctgcagt
    24121 tcctttgcta gataagcgtg ctatcaacgc tcgtatcgag ccaggtgcta ttatccgtga
    24181 ccaggtggaa attggtgaca atgctgttat catgatggga gctgttatca atatcggtgc
    24241 tgaaatcggt gctggaacca tgattgacat gggtgccatc cttggtggcc gtgccatcgt
    24301 tggaaaaaat agccacgttg gtgcaggtgc agttttggca ggtgtgattg agccagctag
    24361 tgctgaacca gtccgtgtcg gagacaatgt tctcatcggg gctaatgcag tggttatcga
    24421 aggagtccaa atcggtagtg gttcagttgt cgcagcagga gctattgtta cccaagatgt
    24481 cccagaaaac gtggtagtag caggtgttcc agctcgtatt atcaaagaaa ttgatgccca
    24541 aactcaacaa aaaacagcgc tagaggatgc gcttcgtacc ttgtaattgt aaaagtaaaa
    24601 aagaggcgga accctttttc cagcctcttt ctgctatatc ggaggacaga tagatgttag
    24661 atttgattca gactagacga gatttacacc agattccaga gattggcttg gaggagttca
    24721 agactcaggc ttatttgctg gatgtgattg agaaattgac tacgggcaag gattttgttc
    24781 aaattcgtac ttggcggaca gggattttgg tctacctgca gggaagtcag ccggagcgaa
    24841 ccattggttg gcggacagat attgatggcc tgcctatcgt cgaacaaaca ggtctacctt
    24901 ttgcttctca acaccaaggt cgcatgcatg cctgtggcca tgattttcat atgaccattg
    24961 ccttaggctg tcttgagcgc gcccttgagg agcaacccaa gaataatctg ctctttctat
    25021 ttcagcctgc tgaagaaaat gaagctggtg gtatgctcat gtatgaggac gatgcttttg
    25081 gagattggtt gccagatcaa ttttatggcc tccatgtccg tccagatttg aaagtcggcc
    25141 agattgcgac taatactcat acactctttg caggaacttg cgaggtgaag atccgtttca
    25201 aaggcaaagg tggccacgca gcttttcctc atgaagccaa tgacgccttg gtggcggcta
    25261 gttactttgt gacccaagtg cagtcagttg ttagccgcaa tgtcaaccca atcgagggag
    25321 cagtggtgac cttcggcgtt tttcaagctg gaaccaccaa caatgtcatc acagacacag
    25381 cttttttaca tggaactatt cgtgccttga cacaggacat gagtctcttg gtgcaaaaga
    25441 gggtaaaaac agttgcagaa ggggttgcag ctgcctttga tatggaagtc gaagtggaac
    25501 tcaaacaagg tggataccta cctgttgaga acaatccagc cttggcgcgt gaactgatgg
    25561 acttctttga cgaaaaagac ggaatcgagt tgattgatat cgaacctgct atgacaggcg
    25621 aggactttgg ttatctcctt tcaaaggttg atggcgtgat gttttggcta ggtatcgata
    25681 gtccctacgc tcttcatcac cctcaaatga gtcctaagga agaagtctta gctattgggg
    25741 tggctgcggt gtctagtttc ctgaaaaaga aggcagcaga atagagggct tgtctatgaa
    25801 atcggaatta cgcaagcaag tcttgcatga aatgaaggct atatctcaag agcaaaaaca
    25861 ggctatagac caagctttaa ccgagcgaat tttacaacac cccttttatc aagaagccaa
    25921 ggtcatcgca acctacctct ctttttctca tgagtttcaa acgcgggaac tgattgagca
    25981 ggcgctgaag gacggcaaga aggttttaat acccaaaact tatcccaagg ggcgcatgga
    26041 ctttgtagtc tatgatccgc agcagttggt aaaaactgcc tttgccttac tggagccaca
    26101 gggagatttg gaagtggtgg atgcatctca gattgatttg attcatgttc ctggcctggc
    26161 ttttacgacg gatggatatc ggattggata tggtggaggt tattatgacc gctatctgga
    26221 acatttttct ggtcatactt tgagtacggt tcatccttgt caaattcagg actttatacc
    26281 tgaaaaccat gatattcctg ttcaggaggt attaattgat gaaggaaatc tttgatagac
    26341 gttaccctgt gacgagtttc ttcctcttag tgacggcctt ggtattttta ctaatgttgg
    26401 tcactgcagg cggaaacttt gacagggcag atacattatt tcgatttgga gccatgtatg
    26461 ggccagctat tcgcctcttt cccgagcagg tttggcgtct cttgtctgcc atttttgttc
    26521 atattgggtg ggaacatttc attgttaata tgctttcact ttattatctt ggaaggcagg
    26581 tagaggagat tttcggttct aagcagtttt tctttctcta tcttttatca ggaatgatgg
    26641 gcaatctctt tgtttttgta tttagtccta aatccttagc agcaggcgcc tctacctctc
    26701 tttatgggct atttgccgcg attattgttc ttcgctatgc aactcgcaat ccttatatcc
    26761 aacagctagg gcaatcctat ctgacacttt ttgtggttaa cattattgga agtgttctga
    26821 ttccaggaat cagcctagca ggccatatcg gtggtgcagt tggtggcgca tttctagcag
    26881 ttatctttcc agttagagga gaaaaacgga tgtataacac cagccagaga ttaggagcgg
    26941 tagtcttgtt cgtaggactc gccattttgc ttttctacaa gggaatggga atgtgacgaa
    27001 gttgaaagaa aatttgtgta atgaaaaaag ataaggcatt ttttgaacct tatcttttta
    27061 ttttatttta ttccttctca gtcaattctt ttccaagttg gatgaggtaa ttcttcaaat
    27121 catctttgac ttgtgggtgt ttgagggcgt agtcgatgga tgttttcatg aagccaaact
    27181 tgtctccgac atcgtaacga gccccattga actcacgagc aaatacacgt tgtgttttat
    27241 tgagggtgtc gattgcatct gtcagctgaa tttcatttcc tgcacctgga gcttggtttt
    27301 cgaggatttg gaaaatttca ggcgtgagga ggtagcgtcc gataatagca aggtcgctag
    27361 gagcgtcctc tggagctggt ttttcaacaa aggtttcaac actgtaaaga ccatcttttc
    27421 cttcgccttg cggagcaata accccgtaag cagatacttc gtcatgaggg actggcatga
    27481 cagcaatggt tgatgcgtgt gtcttttcgt aatcattcat cagttgtttt gtcaaaggaa
    27541 cggccttctc gtctgtgatg tccatcaagt catcacccag cataacaaca aaaggttcat
    27601 ttcctacaaa agctttggct tgtaagacag cgtctccaag accacgaggg tgagtttggc
    27661 ggatgaaatg aaggcgcatg ccagttgctt catctaccaa ttttaacaaa tccgttttgc
    27721 ctttttcctt aagattgtat tcaagctcaa agtttgaatc aaagtggtcc tcaatagaac
    27781 gttttgattt accagtgaca actagaatat cttcaatacc tgatttgaga gcctcttcca
    27841 cgataaactg gatagttggt ttgtctacga ttggcaacat ttctttggca agggccttgg
    27901 ttgctggtaa aaatcgagtt cctagtccag cagcagggat aactgccttt ctaacttttg
    27961 atgtcataaa aatcctttct atagagggtt aagaccactc attttctgct ttaaattcat
    28021 tgttcatgat gtcataaatg gcatctttga tattggttcc gtgataaata acttggtaaa
    28081 tagcctgtgt aatgggcata tagactccaa gttcttgggc tagttcatag gctgctcgag
    28141 tcgttgaaat tccttcgatt accatgccca tattagcttc tatatcagct agggattctc
    28201 ctcgtccgag agcatctcca gctctccagt tacgagagtg gatggaagtt cccgttacga
    28261 tcaaatctcc cacaccagat aagccgctat aggtcaatgg actggccccg agtgctaccc
    28321 ctaggcgggt gatttctgct aaacctcgag cgatgatggc tgccttagca ttatcaccaa
    28381 atccaagacc atgtaaagct ccagcaccga cagcaataat atttttaaga gcaccagcag
    28441 tttcaacccc gataacatcc gtattggtat aaagtcggaa gtagtgatta ctaaatagct
    28501 cctgaacgta ttgagctgtt tgtaaatctt tagaagcagc agttattaaa gttaggtcac
    28561 gcacaatggt ctcttctgca tgactaggcc ctgaaacagc gacgatatca ctacggagat
    28621 gttcaggaat ttcttcttca agaatggttg ataatcgttt atggctatca ggttctaatc
    28681 cctttgatgc gtgcatgatg ataaccttat ggtccaaggt ttgtgcaact tgctgggcaa
    28741 caagtcgtgt cacttttgtt gggacaacaa acaaaatcgc atccacattt ttcaatgttt
    28801 ctgctaagtc ggtgtaggca atgatatttt cgtctagaac gacatcttta aagtagtgct
    28861 tattagtatg gtgtgtatta atttcattga tttgctcggg aagatttccc caaatacgta
    28921 cctcgtgtcc attgtcattt aagacttgtg aaagggcggt tccccaagaa ccaggcccca
    28981 agacggcgac ggtttgtttt tccatgattt cctccttgat agatttcttt ctcttattct
    29041 atcatattct aggggatttt gcacttgcat tactggggta tggtggaaaa atacacaaag
    29101 accgagatga agggataaac tcttagtctt tagttaaagt gagaagaaat ctgatagata
    29161 gtttgattca gtatatgctt ataatagagg taaacaactc aggaagttct tgtaggttgc
    29221 gagagtttgg cgaaaaaatt aaaagattac gtttggctaa aaaaatcagt cgttcagaat
    29281 tttgtggtga tgagtctgaa ttaagtatcc gtcaattaat tagaattgaa aatggagaat
    29341 ccagaccaat actaacaaag ttaaaatata ttgctgaacg tttggaggtt gaagattaca
    29401 agttgatgcc aagttatata gagttggata aggaatacct agaattgaag tatttcttga
    29461 tgaggactcc tacatacgaa gatgaaacta tcacccaaaa gaaagagagt gtttttgata
    29521 agatttttga agagtattat gataggctac ctgaggaaga aagatttatc atcccaaatt
    29581 attcatatct ggcactaacg aactacacag ttcaaaaatt accagaaaag ctagttgaaa
    29641 tactgtcctt ctggtgattt tgcttaattt ctgatgttcg agacatgatt gaggagattt
    29701 gtcttgattt tttcctaaaa aagggcagac tcctcccttg gtttgatgct cgatttttaa
    29761 aataaggtga cttattctgt taggcatgtt ttcccctgcc ctctagctct atatggaaca
    29821 gggagtatca gatttatctg gcagattgta tcaaataagg cttgaaactg ttttgaatag
    29881 gcatatagac ttgagaattt gagaatatgt cgtctagcag tagagacata ttttccggca
    29941 atatgaagga agagacgtcg aaaacgcttg atagtcaagg cctttacttc atcaccagct
    30001 agctgcttta aaaagaggta gagattataa gccagacagc ccatcatcat acgtacttca
    30061 ttcttaatca tggtcgaact atctgtctta tccccaaaga atcctgcttt cctttctttg
    30121 ataaagtttt cagcattccc tcgttcacga tagaattgaa acagctcctc actatctaca
    30181 ctgcttaaat tggtcaaatt ggtcaaatga acttttttac aaccataaaa aagagcactt
    30241 aatcgtactc ttctctttta aggattagtt gaattaattc aaatccacta gttctcctgt
    30301 ttctaggtag actaggcgtt cacaaatgtt agcaatgtaa tcagcgaagc gttccagatg
    30361 ccctatgata taaaggtatt gagttccatt gggaattgag gtttcttggt ctttcataag
    30421 tccaatgatt tcctttgata aggcataata atattggtca atctgttcat ctttttgagc
    30481 aatactaata gcttttgagg cttggtgcaa aggaaaggca accaataaat cggctagcat
    30541 gctgagggat aatttaccca tttggtgtaa ctgttcttcg tcaggggcta gttgattttc
    30601 ttttagttgc aaaacagctt tggcaatgcc tgccatatgg tctcccatac gttcaaggtc
    30661 tgaacaagaa gacatgatgc taatcacaaa tcgaaggtca gacacttgtg gctgctgcaa
    30721 ggctaacaaa cgggcacagg tcaattcgat agcgctttga ccttggttga tagcatgatc
    30781 cttattgata attagctctg ccatctcctt gtctttggag gctaaggcca gtaaggcttt
    30841 tgaagctgtt tcaaggacaa gttgccctag tcctaaaaag gattgttcta attcatgcaa
    30901 ttctaagtca aattgatttc tcataggttt ttccttcctt atccgaaccg tcctgaaata
    30961 tagtcttctg tgcgctgatc ttttggattg gtaaacacgt caacggtatc tccaaattcg
    31021 caaatttctc ctgttaagaa aaaagcagtt ttatctgaaa tacgtgaagc ttgttgcatg
    31081 ttatgggtaa caatgataat cgtataatcc ttttttagtt gctgaatgag gtcttcaatt
    31141 tttaaagtgg agatagggtc taaggctgaa gtcggctcat ccattaacag aatatcaggt
    31201 tctactgcta aagctcgcgc aatgcaaagg cgttgctgct gaccgccaga taaggacatg
    31261 gcactctttt taagatcgtc tttgacttct tcccaaatgg ctgccccttt taaagatttc
    31321 tccactaagg catctaattg ttttttgtct cgaataccat gtgtccttgg accataagcc
    31381 acgttatcat agatagacat ggcaaaggga ttaggctgtt gaaaaaccat ccctacacgc
    31441 ttgcgtagct gattaaggtt gaatttgcta ctataaatat cttgctcatc taagaggact
    31501 tggccttcaa tatggcaaga aggaaccaaa tcgttcatcc gattaagggt ttttagaaaa
    31561 gttgatttgc cacaaccaga tgggcctatc aaggcagtaa tctgtctttc tggtaattga
    31621 atcgaaatat tttttaaggc ttgaaaatcc ccgtaaaata agtctaggtg tctaactgaa
    31681 aatgttccca tactaggaag ctcctttcac aagttttcga gataataagc ttgatagagt
    31741 atttatcatt aaaacagtaa taatcaaaat cacaccggta gcataggctt cattgacatg
    31801 tagcccctca cttgacagca tatacatatg cagggctaga gaacgacctg aagacatgag
    31861 actacttggc gtattggtag aggtacctaa tgtatacatg agggcagctg tctcaccaac
    31921 gatacggcca atagctagta tcactccagc taaaatacct ggcatggcaa ctggtagaac
    31981 aattctaaaa acagtccgta acttacctgc cccaagtcca taacttgctt gacgcatgct
    32041 atcactaaca gataaaaggg cttcttctgt tgagcgaata atgactggca acaccatgat
    32101 gactgaggtt aagattcctg ataacagaga gtattgaaaa cctaagaaga ctacaaagaa
    32161 gagcatgcca aacagaccaa aaacaatgga aggaatccca gacaaggtat ctgaggccaa
    32221 tcgcatgatt ttaacacaaa gggaatcttt ttttgtatat tccacaagat aaaaaccagc
    32281 aaaaatccct atgggcaagg ctaaaagaag agcaccaaag accagaataa cggtggaaat
    32341 aatcgctggc ataagggaaa tgttctcaga agtataagtc caagaaaaga gggatagact
    32401 tagatgaggt aagcctttga tgaggataaa accaatgatt aaaaagagag agccaaaggt
    32461 taaagctgaa aaacaataaa cgagaagttt tagcaggtat ttactcataa gatgattttc
    32521 ctttcaagta ggcaaagtag gcattaatca agagaataag gaaaaagaga actgctgagg
    32581 ttgcaataag ggcttcccta tgctgacctg atgcgtaagc catttccaga acaatattgg
    32641 ttgttaaggt tctggttcct gaaaagagtc cacttggaat aatcggctgg ttgcctgcca
    32701 ccaaaataac tgccatggtt tcacctactg cgcgaccgat tcctaaaata actgctgata
    32761 aaataccaga tctcgcagct ggcaagatga cactaaaaat actccgttca tgactagctc
    32821 ctagagccaa gctaccagaa taatacgttt tgggaactgt tcggatagca gattctgaca
    32881 aactgataat ggttggcaaa atcattattc ctaatagtaa cgaagcggtt aggacactca
    32941 tgccatttcc taaaaaggtt ctaatccaag gcaccaataa ttgtaggccg aaaaaaccat
    33001 aaacaataga tggaatggcc gccatcaagt tgatagctga ttttaagaag ccatagacgg
    33061 gctttggaca ataataaacc ataaacaccg atgtcaagat gcctgttggc accccaatca
    33121 caatcgctcc taaggtaatt aataaggaac caacgatcat tggtaaaata ccatagcttg
    33181 ccggaatgtt cgttggcgac caatcactgc ctaataaaaa acgggcaaag ccgtagttag
    33241 ccatgaaagg taagccatta ctaaaaataa agaaacagat tagcaaaata gctacaacag
    33301 ctactgttgc actcatgaaa aaaattgccc taaaaactgc ttctttgaag gcttgtttta
    33361 tcacatcttg tcctttctag tgaagaaagt aagggagata cgacacctcc ctacttgcct
    33421 tctttatctt attgtacgat gaaacgtctg catctcttta gagatttatg gagcaaacat
    33481 tttatttaat cttgtcccag gtggttaatt tgccactaaa aacgtctgca agttcagcca
    33541 tactgacttg gcttgcctta ttgtcattat tgaccacaac agcaataccg tctaaagcaa
    33601 tagcatcatg ggtgagactc ttaccttctt caggagttaa ttccctagaa accataccaa
    33661 tatcagcggt tttctcctta acagcggtaa tacctgctga agatccatta gaggtaatat
    33721 caatcgtaac ttctggattt tcttttttat aagcttctgc taatttttcc attaaagaag
    33781 atactgaagt ggaacctaca acagacaact tgcctgataa gtgttggctt gtatattctg
    33841 tggtttcggt tttagcttca ataaatttat tatctgtgac cacttgttga ccttgtttgg
    33901 agtggataaa gctgataaaa tcttgaccta gcttggaaag attagaagac caaacaatgt
    33961 tgaagggacg ttgaagaggg tattcaccat ctaaaactgt gtctcgacta gccttgacac
    34021 catcaatctc taaagccttg acagatttcg ttaaagatcc caaggagatg tagccgatag
    34081 cattagcatt cccttgaact gctgagagaa caccttctgt actattttga atcacagctg
    34141 ttttggcagt gttgtcaatt tttttatcac cgtctttttt gagaatccct gtgatttctg
    34201 tgaaggcacc ccgtgttcca gagccatttt ctcgtgaaat cacctcaatc gttcctgaag
    34261 ctgactgttt ggaagcagct gactgattgc cacaggcaac aagcccaaat cctgataagc
    34321 caatggctgc aagagtaagc atttttttga atttcataat aatcaccttt atctctatgt
    34381 atttttcttg tgtaggctta ctacatttat agtctaacaa gtctttgtaa aggtttatcc
    34441 ctgattcatg taaagattgt gtaaagaatc aaaaaaagcc acttttgaaa aatggctgct
    34501 cctaaaaata gctttaaaaa ttattagtcc tgtgcgaaag attggttagg aagaaaaatc
    34561 gtgaagcaac tgcctctgcc aagctgactc gtcaccgtga cttggccacc taataattga
    34621 ctgagttctt tgacaatggc aaggccaaga ccagtgccac cagtttgtct gcttcgacct
    34681 ttattaactc ggtaaaaacg ttcaaaaata cgatcctgct ctaattgact aataccaatc
    34741 cctgtatctg atacagaaat cttaatgcct tcgttcacct tttgggtctt gacctcaatt
    34801 tttccccctt gttcagtgta acggatggca ttggataaaa gattgagtaa gatttgggaa
    34861 agtaattgac tatctgatac gagggtgaca tcatctggca cctgcacctt tagctgtaaa
    34921 tccttcttct tgagctgagg ttgcaagctt tgagtcaaat cctgtacaaa ttctgccaaa
    34981 gaaagggtcg tccattgtat aggcatttgt tgagccttag ataaggtaag aagatgctca
    35041 acaatatgct caagacgcaa actttctttg taaataatgt ctagaaagtc atccttgagc
    35101 gcttcttctt cagctgacat ccccttaatg gtttcagcaa agcccttaat cgaagtaact
    35161 ggtgtcctca attcatggga ggcatttgag acaaaggcta aatttaactt ttcataagtt
    35221 ctaatcgttg ttaaatcata tagcaagacg agcacagctt ccacagattg ggtggggcta
    35281 aaaacgggaa ctgctgtcac ttctaaaatc aagtcaccct catgaaaccc acttacttct
    35341 tgttttaacc ttgttttttg atcaaaggct tggtgaacta aattccgaat atccatccgt
    35401 ttgaggtcat caagtgaact tatgtcgccg tccacatcgg gaaaataatg aggcagagag
    35461 cgactggata ataacatctg accttgagcg gaaactaaaa acgtccccat ggttaggtgc
    35521 gacagaagaa cctccattgt ttcggctaga tccttgtatt gctgatcctg ttgggagact
    35581 ttggttttta ggccagacac atactgagcc aaagacttta agtcttcttg ccctttttct
    35641 aaaaagtatt cactactggt caagagaggt tggtgcaagg tctcaaaagc aacttcccat
    35701 ttccaaaggc aaaagagcca gtagccacct agtcccaaag aaagggctag aagaaagaga
    35761 ccgatgcctt tactgatcca agttaatgcc atccctgcaa tcagaatgag gctaacactt
    35821 agattgacta gccaaaattg aaggtagcgt ttcatctata actccttgaa cttataacca
    35881 taaccccgaa tggttcgaat aaattgaggg gctttaggat tgtcttcaat tttttccctc
    35941 aacttaccaa tatgaacgtc caccaaacgt gtttcctgcc caaagtcata cccccagata
    36001 cgttccaaaa gacgctctct agtcagtgtc atgttgggat gtttcataag atagagcaag
    36061 agttcaaatt cttttggggt caaactcagt aacttattcg ccttgtagac ttcatgacgc
    36121 tcagggtata ctttcaaggt cccaaatagc caagaatcgt cagcgatatt atctgaatca
    36181 tctccttctt gttctccttt agttcgcctg aggacagcct tgacacgcgc cagcaattct
    36241 ctagggctaa aaggcttggt caggtagtca tcagccccta attccaaggc caaaacctta
    36301 tcaaattcat cacttttcgc agaaaccatc ataattggag ttttgacgcc tttggctctc
    36361 agccgcttac aaacttccat gccatctaat tgtggtaaca tgatatcaag caagataaaa
    36421 tcaaagggtt ctgtttctgc caaagctaag gccttccgtc catttgtcac caattgagta
    36481 gaaaagcctt ccttacttaa atggtagtca agcaatttca gaatgtgttc ttcatcatcc
    36541 actaataaga cttgttttgt catctattat ctcctattgg taacattata acacaattat
    36601 cagaaatcct aacattgcta aatcagatta aatttgccta tcaagactag tatctggtca
    36661 aacgctcaat catctccttg tgctctggat aggtcgccag tagatctacc ctttcaaata
    36721 attcaaaatc ctcaaattca aaaccaggaa caacaagaca agaaaccaga gcatcatcct
    36781 tatcaactgt tgatccccaa atagtgccct taggaacaca gtagtgaagt tgttgccctt
    36841 tggatatgtc caggcctaaa gtgactgctt cgtagtgacc atctgctgta atcatgtgaa
    36901 cagtaagtgg ggatcctgca tgaaaatacc agatttcatc tgctgtcaat cggtgaaaat
    36961 gtgaaggatt cgtttcttct aataagaaat aaatactggt ataaagcgcc cttcccttac
    37021 cagcaaggtt tatagtgtct gaagcttttt ttgtttgtct aaaatagcca ccttcaatat
    37081 ggggagctaa ctctagagtt cttatcaagt cttctttatc cgtcggagcc aatgggttga
    37141 agtaactctt gttcaaagtg gttttacgat ttcaagaact cctctcagtt ctgaggacac
    37201 ggtaatgatt gatgcgacgg aagtacaaat caatcgccct aaaaaaagaa ttagcgaatt
    37261 attctggtaa aaaaaatgcc acgctatgaa ggctcaagcg attgtcacaa gtcaagggag
    37321 aattgtttct ttggatatcg ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag
    37381 tcgcagaaat atcggacaag ctggtaaaat cttggctgac aatggttatc aagggctcat
    37441 gaagatatat cctcaagcac aaactccacg taaatccagc aaactcaagc cactaacagt
    37501 tgaagataaa gcctataacc atgcgctatc caaggagaga agcaaggttg agaacatctt
    37561 tgccaaagta aaaacgttta aaatgatttc aacaacctat cgaaatcatc gtaaacactt
    37621 cggattacga atgaatttga ttgctggcat tatcaatcat gaactaggat tctagttttg
    37681 caggaagtct attatttggt taggtgaatt agtgaagcgt ttaggcaagt gtctctggtt
    37741 acgacgtcat ggactctaaa tcgattatat ttaggggtca tgactagtga agcagttagc
    37801 tagttcgcat ataagcggct agcgtctaac aattaggaac tttagttcca ataactttaa
    37861 gattacgacg ttttaggaca taaatcgatc atatttatgt cctaaaacta gtgaagcgcc
    37921 tagccaaagt ccgaatagga tttggcgtta gttacttaga ttgctttgca atcaagtaac
    37981 tttggcgatt tacatcttct ctggcgcttc tactccaagc aagcgaaggg cttctttgag
    38041 aacgactgcg gttgcgtagc tgagggctag acggctgtcg cgttctgggc tttcatccaa
    38101 gatacgtgta tgtgcatagt atttgttaaa ggattgagcc aggctaattg caaatttagc
    38161 aatgatagaa ggttcaaagt tatctgccgc acggttgata atacgtggga agtcttgaat
    38221 gagtttaatg atttcccagc tttcagtatc attcaagcta tagttgccag ctgtttctgg
    38281 tttgaaatcg gctttgcgta agatagattg gatacgagcg taggcatatt gaacgtaagg
    38341 tccagtttca ccctcgaagg ataccatagc ttctaggtcg aagtcgtatc catttgtacg
    38401 gtcagttttg aggtcataga atttaatggc tccaacccca acagcatgtg ctacttggtc
    38461 tttgttttct agttcaggat ttttagcctc gatttggacc ttggcacggc taacagcctc
    38521 tgcaacagta ggctctagca agatgacatt ccctttacga gtagagagct tcttcccttc
    38581 ttttgtaacc agaccaaaag gaacgtgggt aatgtcgtca ctccaatcgt agcccatctc
    38641 ttgcaagaca gctttgagtt gtttaaagtg ggcagattgc tcttgaccaa cgacgtagat
    38701 agatttagca aattggtatt catttttacg gtagagggct gcagccaagt cacgtgtgat
    38761 atagagagtt gcaccatcag acttcttgat gagagctgga tgttcaattc catatttctc
    38821 aagattcaca acttgggcac cttctgattc aagaagtagt cctttttcag aaagaatgtc
    38881 tacaactgca tccatcttat cattgtagaa ggcttctccg ttatagctgt caaattcaac
    38941 cttcaattca ttgtaaaggc ggttaaattc cactaaactt tcatcgcgga accattgcca
    39001 aagagcgaga gcttcctcat ctccattttc aagtttacgg aaccattcgc gcgcttcttc
    39061 atccaagcta gggtcatttt cagcttcagc gttgatgcgg acatagagtt taaggagttc
    39121 atcgattgga tgagctttta cagcttcttc gtcgccccat tttttatagg caacaatcaa
    39181 catcccaaat tgtttacccc agtctcccaa atggttgacc ttgaccgttt gataaccgat
    39241 tttttggaaa atatgtgaca agctatctcc gataacagtt gaacgcaggt ggccaataga
    39301 aaatggttta gcgatattcg gactagacat gtcgataaca acattttctt gtttaccaat
    39361 attttggtca gcatagtgtt ctttttcagt ggtaacagct tgcaatactt gagcagaaat
    39421 ggcagattta tcaaggaaaa agttaacgta aggtcctgtt gcgacaactt tttcaaaggc
    39481 ttggctgttc attttttcag ccagttcagc cgcaatcatt tgtggtgctt tacgttcgac
    39541 ttttgcaaga gaaaaagcag ggaaagcaat gtctcccatt tctgagtttt taggggtttc
    39601 cagtaacttt aaaatagcct cttggtccag gctatcaatg atgctagata attcgctagc
    39661 aatcaattct tttgtattca ttaagagctc ctttttggac ttttctacta ttttatcaca
    39721 attttaaaga aagaagaaaa aatttttgaa atctcctgtt tttttggtat aatatggtta
    39781 taaatatagt tataaatatg cacgcaagag gattttatga gaaaaagaga tcgtcatcag
    39841 ttaataaaaa aaatgattac tgaggagaaa ttaagtacac aaaaagaaat tcaagatcgg
    39901 ttggaggcgc acaatgtttg tgtgacgcag acaaccttgt ctcgtgattt gcgcgaaatc
    39961 ggcttgacca aggtcaagaa aaatgatatg gtgtattatg tactagtaaa tgagacagaa
    40021 aagattgatt tggtggaatt tttgtctcat catttagaag gtgttgcaag agcagagttt
    40081 accttggtgc ttcataccaa attgggagaa gcctctgttt tggcaaatat tgtagatgta
    40141 aacaaggatg aatggatttt aggaacagtt gctggtgcca ataccttatt ggttatttgt
    40201 cgagatcagc acgttgccaa actcatggaa gatcgtttgc tagatttgat gaaagataag
    40261 taaggtcttg ggagttgctc tcaagactta tttttgaaaa ggagagacag aaaatggcga
    40321 tagaaaagct atcacccggc atgcaacagt atgtggatat taaaaagcaa tatccagatg
    40381 cttttttgct ctttcggatg ggtgattttt atgaattatt ttatgaggat gcggtcaatg
    40441 ctgcgcagat tctggaaatt tccttaacga gtcgcaacaa gaatgccgac aatccgatcc
    40501 ctatggcggg tgttccctat cattctgccc aacagtatat cgatgtcttg attgagcagg
    40561 gttataaggt ggctatcgca gagcagatgg aagatcctaa acaagcagtt ggggttgtta
    40621 aacgagaggt tgttcaggtc attacgccag ggacagtggt cgatagcagt aagccggaca
    40681 gtcagaataa ttttttggtt tccatagacc gcgaaggcaa tcaatttggc ctagcttata
    40741 tggatttggt gacgggtgac ttttatgtga caggtctttt ggatttcacg ctggtttgtg
    40801 gggaaatccg taacctcaag gctcgagaag tggtgttggg ttatgacttg tctgaggaag
    40861 aagaacaaat cctcagccgc cagatgaatc tggtactctc ttatgaaaaa gaaagctttg
    40921 aagaccttca tttattggat ttgcgattgg caacggtgga gcaaacggta tctagtaagc
    40981 tgctccagta tgttcatcgg actcagatga gggaattgaa ccacctcaaa cctgttatcc
    41041 gctacgaaat taaggatttc ttgcagatgg attatgcgac caaggctagt ctggatttgg
    41101 ttgagaatgc tcgctcaggt aagaaacaag gcagtctttt ctggcttttg gatgaaacca
    41161 aaacggctat ggggatgcgt ctcttgcgtt cttggattca tcgccccttg attgataagg
    41221 aacgaatcgt ccaacgtcaa gaagtagtgc aggtctttct cgaccatttc tttgagcgta
    41281 gtgacttgac agacagtctc aagggtgttt atgacattga gcgcttggct agtcgtgttt
    41341 cttttggtaa aaccaatcca aaggatctct tgcagttggc gactaccttg tctagtgtgc
    41401 cacggattcg tgcgatttta gaagggatgg agcaacctac tctagcctat ctcatcgcac
    41461 aactggatgc aatccctgag ttggagagtt tgattagcgc agcgattgct cctgaagctc
    41521 ctcatgtgat tacagatggg ggaattatcc ggactggatt tgatgagact ttagacaagt
    41581 atcgttgcgt tctcagagaa gggactagct ggattgctga gattgaggct aaggagcgag
    41641 aaaactctgg tatcagcacg ctcaagattg actacaataa aaaggatggc tactattttc
    41701 atgtgaccaa ttcgcaactg ggaaatgtgc cagcccactt tttccgcaag gcgacgctga
    41761 aaaactcaga acgctttgga accgaagaat tagcccgtat cgagggagat atgcttgagg
    41821 cgcgtgagaa gtcagccaac ctcgaatacg aaatatttat gcgcattcgt gaagaggtcg
    41881 gcaagtacat ccagcgttta caagctctag cccaaggaat tgcgacggtt gatgtcttac
    41941 agagtctggc ggttgtggct gaaacccagc atttgattcg acctgagttt ggtgacgatt
    42001 cacaaattga tatccggaaa gggcgccatg ctgtcgttga aaaggttatg ggggctcaga
    42061 cctatattcc aaatacgatt cagatggcag aagataccag tattcaactg gttacagggc
    42121 caaacatgag tgggaagtct acctatatgc gtcagttagc catgacggcg gttatggccc
    42181 agctgggttc ctatgttcct gctgaaagcg cccatttacc gatttttgat gcgattttta
    42241 cccgtatcgg agcagcagat gacttggttt cgggtcagtc aacctttatg gtggagatga
    42301 tggaggccaa taatgccatt tcgcatgcga ccaagaactc tctcattctc tttgatgaat
    42361 tgggacgtgg aactgcaact tatgacggga tggctcttgc tcagtccatc atcgaatata
    42421 tccatgagca catcggagct aagaccctct ttgcgaccca ctaccatgag ttgactagtc
    42481 tggagtctag tttacaacac ttggtcaatg tccacgtggc aactttggag caggatgggc
    42541 aggtcacctt ccttcacaag attgaaccgg gaccagctga taaatcctac ggtatccatg
    42601 ttgccaagat tgctggcttg ccagcagacc ttttagcaag ggcggataag attttgactc
    42661 agctagagaa tcaaggaaca gagagtcctc ctcccatgag acaaactagt gctgtcactg
    42721 aacagatttc actctttgat agggcagaag agcatcctat cctagcagaa ttagctaaac
    42781 tggatgtgta taatatgaca cctatgcagg ttatgaatgt cttagtagag ttaaaacaga
    42841 aactataaaa ccaagactca ctagttaatc tagctgtatc aaggagactt ctttgacaat
    42901 tctccacttt tttgctagaa taacatcaca caaacagaat gaaaaggagc tgacgcattg
    42961 tcgctccctt ttgtctattt tttaaggaga aagtatgctg attcagaaaa taaaaaccta
    43021 caagtggcag gccttggctt cgctcctgat gacaggcttg atggttgcta gttcacttct
    43081 gcaaccgcgt tatctgcagg aagtattaga cgccctcctt actgggaaat atgaagctat
    43141 ttatagtatc ggggcttggt tgattggtgt ggccgtagtc ggtctagttg ctggtggact
    43201 caatgttgtc ctcgcagcct atattgccca aggagtttca tccgaccttc gggaggatgc
    43261 cttccgtaaa attcaaacct tttcttatgc tgatattgaa caatttaatg cgggaaatct
    43321 agtcgttcga atgacaaatg atatcaacca gattcagaac gttgtcatga tgaccttcca
    43381 aattcttttc agacttcccc tcttgttcat cggttcgttt atcctagcgg ttcaaacctt
    43441 accttctctg tggtgggtga ttgttctcat ggtagtcttg atttttggtt tgactgctgt
    43501 catgatggga atgatggggc ctcgttttgc caagtttcaa acccttcttg agcgcattaa
    43561 tgccattgcc aaggaaaatt tacgtggcgt tcgtgtggtc aagtcctttg tccaagaaaa
    43621 agagcaattt gctaagttta cagaggtctc agacgagctt tttggtcaaa acctttacat
    43681 tggttatgcc ttttcagtag tggaaccctt tatgatgttg gttggttacg gggcggtctt
    43741 cctctctatt tggctggtcg cgggaatggt tcagtcggat ccgtctgttg ttggttccat
    43801 cgcttctttt gttaattacc taagccagat tatctttacc attgttatgg ttggattttt
    43861 gggaaattct gtcagccgtg ccatgatttc catgcgtcgt attcgagaaa ttcttgacgc
    43921 agagccagct atgaccttca aggatatccc agatgaagag ttggttggaa gccttagctt
    43981 tgaaaatgtg acctttacct atccaatgga caaggaaccg atgctgaaag atgtgagctt
    44041 tactattgaa cctggtcaaa tggttggtgt agttggagcg actggtgcag gaaagtcaac
    44101 cttggctcaa ttgattccac gtctctttga tccacagaac ggggccatta aaatcggtgg
    44161 caaggatatt cgagaagtga gtgaaggaac cctgcgtaaa acagtttcca tcgttctcca
    44221 acgtgccatt ctttttagtg gaacgattgc agataacttg agacagggga agggaaatgc
    44281 tactctattt gaaatggagc gcgcagccaa tattgcccag gctagtgaat tcattcatcg
    44341 tatggagaaa acctttgaaa gtccagttga agaacgggga accaatttct ctggtggaca
    44401 aaaacaaagg atgtcgattg cgcgtgggat tgtcagcaat ccacgtattc tgatttttga
    44461 tgattcgacc tcagccttgg atgccaaatc agagcgcttg gtgcaagaag ctttgaataa
    44521 ggacttgaag gggacgacaa ccattattat tgctcaaaaa attagctcgg ttgtccatgc
    44581 agacaagatc ttggttctaa atcaaggacg attgattggt caaggcacgc atgcagactt
    44641 ggttgccaac aatgccgttt accgtgaaat ctatgaaaca cagaaatgaa agacaaacta
    44701 taagaaaagt caatagtttt atctaaacta tttcttattt caatttgatg atttggcgat
    44761 gattttagag cacg
//
LOCUS       DBEJAQ010000012        42552 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.012, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000012 DBEJAQ010000000
VERSION     DBEJAQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 42552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.012"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..1954)
                     /gene="ftsH"
                     /locus_tag="ACL297_000707"
     CDS             complement(<1..1954)
                     /gene="ftsH"
                     /locus_tag="ACL297_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGR0071002.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAHHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
     gene            complement(1970..2512)
                     /gene="hpt"
                     /locus_tag="ACL297_000708"
     CDS             complement(1970..2512)
                     /gene="hpt"
                     /locus_tag="ACL297_000708"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGR0071003.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2516..3793)
                     /gene="tilS"
                     /locus_tag="ACL297_000709"
     CDS             complement(2516..3793)
                     /gene="tilS"
                     /locus_tag="ACL297_000709"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGR0071004.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3790..5058)
                     /locus_tag="ACL297_000710"
     CDS             complement(3790..5058)
                     /locus_tag="ACL297_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HGR0071005.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5051..5173)
                     /locus_tag="ACL297_000711"
     CDS             complement(5051..5173)
                     /locus_tag="ACL297_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HGR0071006.1"
                     /translation="MENLLDVIEQFLSLSDEKLEELADKNQLLRLQEEKERKNA"
     gene            complement(5178..5546)
                     /locus_tag="ACL297_000712"
     CDS             complement(5178..5546)
                     /locus_tag="ACL297_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGR0071007.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5539..5805)
                     /locus_tag="ACL297_000713"
     CDS             complement(5539..5805)
                     /locus_tag="ACL297_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGR0071008.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5863..9372)
                     /gene="mfd"
                     /locus_tag="ACL297_000714"
     CDS             complement(5863..9372)
                     /gene="mfd"
                     /locus_tag="ACL297_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGR0071009.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(9373..9942)
                     /gene="pth"
                     /locus_tag="ACL297_000715"
     CDS             complement(9373..9942)
                     /gene="pth"
                     /locus_tag="ACL297_000715"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGR0071010.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(10014..11129)
                     /gene="ychF"
                     /locus_tag="ACL297_000716"
     CDS             complement(10014..11129)
                     /gene="ychF"
                     /locus_tag="ACL297_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGR0071011.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11213..11407)
                     /locus_tag="ACL297_000717"
     CDS             complement(11213..11407)
                     /locus_tag="ACL297_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HGR0071012.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11472..12608)
                     /gene="dnaN"
                     /locus_tag="ACL297_000718"
     CDS             complement(11472..12608)
                     /gene="dnaN"
                     /locus_tag="ACL297_000718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGR0071013.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12767..14128)
                     /gene="dnaA"
                     /locus_tag="ACL297_000719"
     CDS             complement(12767..14128)
                     /gene="dnaA"
                     /locus_tag="ACL297_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGR0071014.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14341..15099)
                     /locus_tag="ACL297_000720"
     CDS             complement(14341..15099)
                     /locus_tag="ACL297_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGR0071015.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15157..16338)
                     /locus_tag="ACL297_000721"
     CDS             complement(15157..16338)
                     /locus_tag="ACL297_000721"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HGR0071016.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16521..17000
                     /gene="rlmH"
                     /locus_tag="ACL297_000722"
     CDS             16521..17000
                     /gene="rlmH"
                     /locus_tag="ACL297_000722"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGR0071017.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17044..17117
                     /locus_tag="ACL297_000723"
     tRNA            17044..17117
                     /locus_tag="ACL297_000723"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17078..17080,aa:Arg,seq:tct)
     gene            17282..17407
                     /gene="comC"
                     /locus_tag="ACL297_000724"
     CDS             17282..17407
                     /gene="comC"
                     /locus_tag="ACL297_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HGR0071018.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIKGGEMRISRIILDFLFLRKK"
     gene            17428..18753
                     /gene="comD"
                     /locus_tag="ACL297_000725"
     CDS             17428..18753
                     /gene="comD"
                     /locus_tag="ACL297_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system sensor histidine kinase ComD"
                     /protein_id="HGR0071019.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYIFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18750..19502
                     /gene="comE"
                     /locus_tag="ACL297_000726"
     CDS             18750..19502
                     /gene="comE"
                     /locus_tag="ACL297_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system response regulator
                     transcription factor ComE"
                     /protein_id="HGR0071020.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19554..19625
                     /locus_tag="ACL297_000727"
     tRNA            19554..19625
                     /locus_tag="ACL297_000727"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19587..19589,aa:Glu,seq:ttc)
     gene            19631..19704
                     /locus_tag="ACL297_000728"
     tRNA            19631..19704
                     /locus_tag="ACL297_000728"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19665..19667,aa:Asn,seq:gtt)
     gene            complement(19745..20287)
                     /locus_tag="ACL297_000729"
     CDS             complement(19745..20287)
                     /locus_tag="ACL297_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGR0071021.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDVIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20466..22069
                     /locus_tag="ACL297_000730"
                     /pseudo
     CDS             20466..22069
                     /locus_tag="ACL297_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(22099..24651)
                     /locus_tag="ACL297_000731"
     CDS             complement(22099..24651)
                     /locus_tag="ACL297_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HGR0071022.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(24713..26335)
                     /locus_tag="ACL297_000732"
     CDS             complement(24713..26335)
                     /locus_tag="ACL297_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGR0071023.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            26541..27566
                     /gene="trpS"
                     /locus_tag="ACL297_000733"
     CDS             26541..27566
                     /gene="trpS"
                     /locus_tag="ACL297_000733"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGR0071024.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            27718..29196
                     /gene="guaB"
                     /locus_tag="ACL297_000734"
     CDS             27718..29196
                     /gene="guaB"
                     /locus_tag="ACL297_000734"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGR0071025.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(29247..30344)
                     /gene="recF"
                     /locus_tag="ACL297_000735"
     CDS             complement(29247..30344)
                     /gene="recF"
                     /locus_tag="ACL297_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGR0071026.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(30347..30715)
                     /gene="yaaA"
                     /locus_tag="ACL297_000736"
     CDS             complement(30347..30715)
                     /gene="yaaA"
                     /locus_tag="ACL297_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGR0071027.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            30874..32124
                     /locus_tag="ACL297_000737"
     CDS             30874..32124
                     /locus_tag="ACL297_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGR0071028.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            32121..33404
                     /locus_tag="ACL297_000738"
     CDS             32121..33404
                     /locus_tag="ACL297_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGR0071029.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            33446..34267
                     /gene="rodZ"
                     /locus_tag="ACL297_000739"
     CDS             33446..34267
                     /gene="rodZ"
                     /locus_tag="ACL297_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGR0071030.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFIFFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            34278..34823
                     /gene="pgsA"
                     /locus_tag="ACL297_000740"
     CDS             34278..34823
                     /gene="pgsA"
                     /locus_tag="ACL297_000740"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGR0071031.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            34820..35647
                     /locus_tag="ACL297_000741"
     CDS             34820..35647
                     /locus_tag="ACL297_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGR0071032.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            35632..36471
                     /locus_tag="ACL297_000742"
     CDS             35632..36471
                     /locus_tag="ACL297_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGR0071033.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            36464..37258
                     /locus_tag="ACL297_000743"
     CDS             36464..37258
                     /locus_tag="ACL297_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGR0071034.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            37317..38135
                     /gene="mreC"
                     /locus_tag="ACL297_000744"
     CDS             37317..38135
                     /gene="mreC"
                     /locus_tag="ACL297_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGR0071035.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38135..38629
                     /gene="mreD"
                     /locus_tag="ACL297_000745"
     CDS             38135..38629
                     /gene="mreD"
                     /locus_tag="ACL297_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGR0071036.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            38723..39901
                     /gene="pcsB"
                     /locus_tag="ACL297_000746"
     CDS             38723..39901
                     /gene="pcsB"
                     /locus_tag="ACL297_000746"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HGR0071037.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40125..40904
                     /gene="rpsB"
                     /locus_tag="ACL297_000747"
     CDS             40125..40904
                     /gene="rpsB"
                     /locus_tag="ACL297_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGR0071038.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            40983..42023
                     /gene="tsf"
                     /locus_tag="ACL297_000748"
     CDS             40983..42023
                     /gene="tsf"
                     /locus_tag="ACL297_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGR0071039.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            42338..>42552
                     /gene="tnpB"
                     /locus_tag="ACL297_000749"
     CDS             42338..>42552
                     /gene="tnpB"
                     /locus_tag="ACL297_000749"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGR0071040.1"
                     /translation="MDSLAYVVKTHFELDSFSGQVFLFCGERKDRFKALYWDGQGFWL
                     LYKRFENGKLTWLSTEKDVKALAPEQV"
BASE COUNT        13335 a         7331 c         7855 g        14031 t
ORIGIN      
        1 tttcgtcatt catttttgac tttacttcat cataggatag tgcatgagat tcctcttcta
       61 ctgcttcagg catctttcct gtttcgtaaa gagctttaat ttgtgtacta tccaatgttt
      121 cgtatttcaa taatgcttct gcaattaact tgtgagtttc acgatttgac tgaataattt
      181 cagcagcttt atttcgtgcc tcatttaata atgaacgaac ctcttcatca atttcataag
      241 ctgtttgttc tgaaattgat ttttgaggac tctgtgcacc aagcatagca tggtttcctt
      301 catattgtac tgggccaagt ttttcactca taccgtactc tgtaaccatt gcacgtgcca
      361 tttgtgtcgc ttgttcaaag tcgtttgaag ctcctgtagt ttggacatta aagataattt
      421 cttcagctac acgtccaccc attaagccag ccaattgctc tttcatatct tctttagata
      481 gaagcatttg atcctcttta ggaagtgcaa tcatgtatcc gcctgcacgg ccgcgtggta
      541 caattgtaac cttatggaca acgcgagcat tcgacaagac tagaccaaca atggtatgtc
      601 ctgcctcatg gtgagcaacc aattctcgtt ctttttgtga aactgtctta tctttcttag
      661 aaggtccagc aataactcta tcttctgctt catcaatatc tgaagcatca attatcgatt
      721 tattgcgacg agcagcaact aaagctgctt cattcaagac attctctaaa tcagcaccaa
      781 caaagcctgg agtttgttga gccactaatt tcaaatcaac atcttctgct aaaggcttgt
      841 tcttagcgtg aactttcaag attgcttcac gacctttaac atcaggacgg ccaaccaata
      901 cttttctatc aaaacgtcct ggacgcaaaa gggcaggatc aagtacatct gaacggtttg
      961 tcgcagcgat gacgataatc ccttcatttc cctcaaaacc atccatctca atcaaaagtt
     1021 ggttcaaggt ttgttcacgt tcgtcattac ctccgccgag accgactcca cgttgacgtc
     1081 caacagcatc aatttcatcg ataaagatga tagctggtgc tgcttttttg gcatcctcaa
     1141 aaagagagcg aacacgacta gctccaactc cgacaaacat ttctacaaag tcagaacctg
     1201 agatactaaa gaatggaaca cctgcttctc cagcgactgc cttagcaagc aaagtcttac
     1261 ctgtccccgg aggtccctcc aaaagaacac ctgctggaat acgggctcca agttttgtga
     1321 atcgttttgg atcttttaag aactcaacaa cttcaactag ttcttgtttt tcttcctcag
     1381 ctccagcaac atctgaaaat cttactttaa tatcttcttt atttgctgct ttagccttac
     1441 tacgtccaaa actcattgga ttacggccat tgcctcctcc catatttccc atcatagaga
     1501 ataggaagaa gaatagaatt ccaaatggca caatggatac gagtagatta atccatatac
     1561 cacttgaact ttcatgctta acagttactt ctgctttatg gtcagtagca agtttttgca
     1621 attctgatac ggtagtatct gcaggaagaa tagtgctggt aaatttctct accttagtaa
     1681 cagatggcgt gaaaaactga atacctgttc cttctttact tgttttagga tttttataga
     1741 caccagaaac ttcgataaca ctaccatttg gttggtaagt taattctttt acattaccat
     1801 cggtaatttc ttgtaccaac tcagtatagt tgatttgctg acttcctcct gagttattcc
     1861 cagaatagaa atactggaat cctgtcacaa ggaaaaagat aaataataac catagaaaag
     1921 gatttttaat taaaccatta ttttgttttt tcattaaaga ttattctttc taatttgaat
     1981 acacttcctc tttcaatact ccaatataag gaagattacg ataattttct ttgtagtcta
     2041 aaccataacc tactacaaac tcatttggga tagtaaagca ggtatagtct gcctcaattt
     2101 ctacaacacg tccttctggt ttatccaaca aggttgcaat tttaacagaa gctgcttctc
     2161 tttctttaaa catatctcgc aaattcttca aagtttgacc tgtatcaatg atatcttcta
     2221 caaatagaac atgtcttcct ttgatatctt gagtcacatc ttgtttaata ttgataacac
     2281 cactacttgc tgttccacca tggtagctag aaaccatcat gaagtccatt tcaatatgtg
     2341 tatcaatatg tttgaccaat tcagccataa aaggaataga tccttttaaa atcccaacta
     2401 agattggatt ttttcctgca tagtctttag ttaattgagc acctagtttt ttagctgctt
     2461 ctgtaatttc atcgtgtgaa acgaggactt ttttaatatc gttttctaac attttttacc
     2521 tatctatttt ttctatataa agtacagtgt tcattatatc atttttcgtt tttttactca
     2581 aattattggt cgcaattccc aaaattgaga caatttcacc aaattgctca ataataagag
     2641 cagagtttct cttttccata gggattttca aatcaataaa taaacgtctg agtttttttc
     2701 tatgcccatt ttgaatcaaa acatctcctg tttttcgatg acgaatgtgt atggatgttt
     2761 cacgtgaaac aggtatttgt tgaattaatt caccttctaa tggaagtcca aaggaaaata
     2821 aatatccttg ataagctacc tgattttgat agtgtaacac aagttcatct tcctcttcat
     2881 cagcctgcgg actgatttta caaatctgaa actgttggta ctcttttatc aattcatagc
     2941 catttttaat cggatgacga tactggcttt tagattttaa aatctgctga acttcagcaa
     3001 actgagcttt tgtaagattc aaatctggaa aacgattcag ataagtttga agtaaaactc
     3061 tttgtgtaga ctcagagtaa gaaaataact gctgtaaatt ttccacatta atattgttag
     3121 ataattcagc tattgccaaa tcataatcta aaatttcatt gccgatgcct aagattgcat
     3181 ccctaaatcg aggattttct ttttccaatt ctggtaagta agaatttcga atacgatttc
     3241 gaaaataatg attctcctga tttgatgtat cttcaaagtg aaaaattgat ggaaagtctt
     3301 ttttctgaaa atgcaagaag ggacgaatga tttctatctc tccgactact tgcttctcct
     3361 taattcctga tagatagcgc aagcgagttc ctcgaatcaa gcgcatcaaa atcgtttcca
     3421 cctgatcatc agcatggtgg gcagtgacta aagctgtcgc acctgtcttt ttcatgacct
     3481 cttgaaaaaa atcataacga aaatttcgtg cacgcgcttc tgaaaattct cctgaaaaat
     3541 tgctgatata aataggaagc tctgcttcag cagccaactt ccttaattcc ttttcttccc
     3601 aatctgattc aattctctgc ttatgattca catgagctag aatcaattca atctctaact
     3661 ctttttgata agtagacaat accttaaata gaaacatgga atctaatcca ccagaaagag
     3721 ctagaaccgc cttagcatgc tttttgaaat atcccttctt gagaaaatga tttaaaaaat
     3781 ctggttccct cattttagaa cctcataaac atccttggct atcttagaaa tcgtatcata
     3841 atcagaattc ttagtgaaaa tagaaagaat aaatggagaa tctgcataga caacacccgt
     3901 atcatgctta aattcatccg catctccaat tttatgagct actttaacag aaacaccttt
     3961 ggcaattcgc tgactatcaa aatctgtttt agtcaaagac tctagcacaa atccattttg
     4021 attataaata gcttccataa acttcccggc catcttagaa gaaatcaatt tttcttttgg
     4081 atcccaatca tctcccataa tggcagacat cttggatttg aatgtggcat cagattggtt
     4141 tgaaatgtaa tatcccaata gattatgagc tacattatca gattcttttg atactttcgt
     4201 aattaaatcc tttaaagaat attctttatt atcttctttt ttaggaagac taccacttcc
     4261 ctctggttta taagaacctg gaaaatcatt gactgcagat acgtatttta cagtcgtatc
     4321 taactgataa agaccctcat ttattttttc ttgcgtataa tagagatagg agagtttcaa
     4381 aacgctggct gcatacatct tttcatcttg attgatacca gcttcttttc cagtagtcag
     4441 ttgcttaaca taaatagaga aagaatcttt ctgatatttt tcagataaca tttcttgaac
     4501 tttactcatc cgattatctt cttcagaagt tgattcttta gctacccatc cagcctgatc
     4561 aatatgtaga aattctcttc cttctacaaa catggtcttg tcgattgata cttgcgaata
     4621 agctgataag gatgatttca cttcttttaa atcataagga ctattgtaca gtttaaagtc
     4681 agattctaac catgcttttt ttattgttgg agttacctct gattgatcat ataaaaatcg
     4741 tttgtccgca gctataaatt gatgatttga tagcttaaat actggaattc cttgtttatt
     4801 taagcgccac tcggttattt gaaaacttgt tttgggagtc aatttcccag attccactac
     4861 taaatcttca ttcgcataaa caggaacctc tccataaacc atgggagaac ttaatttttc
     4921 tctaaaataa atactaaagt cagattgtga aaggtaataa atttctttcg aagtatagac
     4981 gacttctttt tctgtgctaa cgacttttga aatggtcaaa aaacttggta gcaacaaaat
     5041 aattaagaat ttacgcattc ttcctttcct tttcttcttg taaacgcaat aattgatttt
     5101 tatcagccaa ttcttccagc ttttcatctg acaaactcaa aaattgctct attacgtcta
     5161 ataaattttc cattttatca cctttgaagc aagtcaggaa tcgtataaac tttttccctc
     5221 gacttagaat aatagtactt cgctcgtgta tatttagcag cataatcttc atctttcaac
     5281 ttggtagcaa atgctgtctc cttatccttt tcatcactca aagtttgata ctgagtttgc
     5341 aagtctgcta attgctgacg tctttggagt aattgctgat aactctgcgc taaattaaaa
     5401 gttggcaaga taaataatag cataatcaaa atcaataccc ctcccataaa acgattccgt
     5461 ttttgtcgtt ctttcatcag gtagcgacga cgttggtatt cattttgaat aaaagaatta
     5521 ttcaattgta caatattttt agacattttc ttctacccgt gtttcactga taatttcata
     5581 cattcctgct gcatcttctt tttttgtact atctttcatc tctagtactt ttacaagcag
     5641 caacttattg ccaaagcgaa tttcaacttg gtcattaact ttcaagtccg ttgaactttt
     5701 ggccaagatt ccattaacct tgattctacc tttatctgct acttcctttg cgactgtacg
     5761 acgcttgata attcgcgata cttttaaata tttatctaat ctcatcctta ttacctcaat
     5821 ttattattgt accattttta tctattttat agaagaaaaa tatcaaatgg aattttcttc
     5881 cttaaactct tttatctcta ataaactttc tccaaaaatc agcagacctt ctaaaatttc
     5941 ataatctttc ttattttgga catcaaatac aagctccatt aatcccttat tctcagcgat
     6001 gcctgctttt aagttcgtta cggataaagc tttaaaataa tcttgagcta aaaacagccg
     6061 ttgagtgact ttttcaaatt gaattgtaat tttattatct tttctttcca cacgttgaac
     6121 aaagaccttg tccaagtatg atttaaccaa accaatctct aaaagatagg ctactacatc
     6181 tgggtattct ccaaaacggt ctatcaactc ctcttgtaac tcttcataat tgacacggtt
     6241 gtcaatttga cgaattttct tgtaaatttc aatcttatgt cgttgatcag aaatataagt
     6301 atcaggaaga taggcatcaa tttgcaaaat caactcagca ttccctttgg ttcttgtgtt
     6361 agcgttagca ttaccgtttc gtttagcaat agcttcctct aataactgcg aatacaattc
     6421 aaaaccaaca gaatcaatga aaccagactg ggattttcct aaaagatttc ctgctccacg
     6481 aatcgaaaga tctcgcattg caatcttaaa gccagagccc aattctgtaa atcctttaat
     6541 cgcttctaat ctcttttcag agacttcact gattgatttt tctggacgat acatgagata
     6601 agcataagca atacgattac tacgaccgac tcttcctctt aactgatata aggttgacaa
     6661 gcccatatgg tccgcatttt caataaataa agtattagca tttggaatgt ccacccctgt
     6721 ctcaataata gtagtcgtca ccaagatatc gtattgtccc tcaataaagt ctaatagagt
     6781 attttccaac tggacttcac tcattcgacc atgaacatat ccaatcgaag cctccggaat
     6841 caactcctgt aattctgaaa ccttctgaac aattgtgtca actttgttgt aaagataata
     6901 aacttgacct ccacgctcca tttcacgcaa gacagcatca cgaatgacac tatcattctt
     6961 ttccaaaaca taggtctgaa caggatagcg attagtcggc ggagtttcaa taacagataa
     7021 atctctgatt cccagcatag acatatggag ggtacgaggg attggcgtag cggtcaaggt
     7081 taggacatcc acttgtttct tcagttcttt caaagtttcc ttatgcttga caccaaatcg
     7141 ctgttcctca tcaataatca tcaagcccaa atcagcaaac acaacatctt ttgacaaaac
     7201 acgatgtgtt ccaatcaaaa tatcgacttg accgtttttc aatttttcaa gtgttgcagt
     7261 ctgctctttt ttacttctaa agcgactcaa cacatcaata ttaactgcaa aattttggaa
     7321 tcgttcctta aaattcgtat agtgctgttg cgctaaaacc gtcgtcggaa ctagaatgac
     7381 aacctgtttg tgatcattga ctgctttaaa ggctgcacgc atagcaactt cagtctttcc
     7441 aaaaccaaca tccccaacta aaagtcgatc cattggctga gaagcctgca tatccctctt
     7501 gatttcctca atactacgaa gttgatcatc cgtttcaaca taagggaaag catcatcaaa
     7561 ggcatcttga tcatcatcat cagctgagaa agcaaaaccc ttcaactgac tacgttcaga
     7621 gtagagtttg attaaatcat cagctatatc ctctacctgg ttcttaacct tttgcttggc
     7681 ctttttaaaa tgaccgtcat ttaatttatt gagttttgga gctttaccat cacttgaaat
     7741 atatttggac agtagatgaa tctgttccac ggggatagaa atttgatcac cattttggta
     7801 ttggacactg acataatcgc gatgaattcc cttgatttca atggtttcaa ttcctagata
     7861 ttgaccaatc ccatggatat gatggacaac atagtcccct ttttcaagtt cattgtaatc
     7921 ttttaatctc tctgcatttg aaacatgttg tcttcgaaaa cgacgcttta atttcttttg
     7981 aaaaatctca tgttcagtta tcaataaaat cttttcatct acaaaatgaa aaccatgtct
     8041 gagattaccc tctattaagt ttacagattc tttacagata tttgtcttat ctctagaatc
     8101 caatttaatc tgatattcct ctaacatatc ctccaatgtt ttacttccca ttgaattgct
     8161 agactgcaga ataatggtgt aatccatttt tttatatcgt tcaatttctt cttttagaaa
     8221 agaaaactga ttgaaaaatt cctgcatagg atattgattg aattgataaa ttttgtcaaa
     8281 tttgagattt cctaaaccct tttgaagatt agagaaaaag gtcactggac tttgtttttt
     8341 atagatttgt tcaatatcag aaaaatactg catatcagaa aatgctttac tattctgtaa
     8401 ttcttctgta aagtactgcg ctaagtctct ttcaaagact tcatactgat tcatcaattt
     8461 ttgataatca tcaaagaata ttggggtatc tttttcaata taatcaaaga cagtccatgt
     8521 cttatcatag cacaaagata aaaacttccg agagtctgca tgactttgtt tttggtgaaa
     8581 acttgaaaga atttcttcta ggtatgattt caaaataggt gataaagttt ttgaaatttg
     8641 tttttctaaa gctgactgtc ctcgttgata atccttttct ctcaaaagca tatcactagc
     8701 tggaaagata gtgagttctg tcttattttc tttcgataat tgtgtttcta cttcaaatga
     8761 cctgatacca tcaatttcat caccaaaaaa ctcaattcga caaggttcta actgggatat
     8821 ttcaaaaata tctaaaatat ctcctcgaag actaaattcg ccctgagttt gtacttgagt
     8881 aacttttcga tagccatttt cctttaactg atggataaac gcgtgttgat catattcttc
     8941 accaactgag atttttacaa tactatcttt gaatgcattg ggagacggta aaatcaatcg
     9001 acttgctacg atattacaaa ctaaaatccc tttcttagat gaatcagtca aaaaacgcaa
     9061 ggcttcaacc cgtgaaataa ttttttcctg tgaagacatc aaaaactcca ccataggagc
     9121 atcatctacc aaaaatggat agacgagttc ctcacccaag atagatataa gatcactaac
     9181 aagtccttct gcttctccat aagttgacgt caataacaca atcctatctt ctttttctaa
     9241 actgcttgca attgcaagag ccttagtaga tgttgataaa ccaagtatta gttgtctttt
     9301 cttatctgtt aaattttgat gccatttttt aatctgatca ttttctgaga ataaatctaa
     9361 taaggtcacc atttatccgt tatacctctg cattgttttc tcaaaatttt tctcttgtaa
     9421 atagtagttt acagaatcgt caactttgtc aatagactgt aaaataccga tataatcatc
     9481 cctgtcaaac ttactcaaaa catgatgaac aactgacata ccatttttag gtcttccaat
     9541 tccaatctta acacggttaa agacctgagt tcctatatgt tgaataatag acttgatacc
     9601 attatgacca cctgctgagc cttttgctct taaacgaatt ttcccaactt ccatgtcaag
     9661 atcatcgtaa atgataagta aatcgtcaat atccaaacca tagtaagtta ataaagcatg
     9721 aactgctttt ccactttcat tcataaaggt cgttggttta accagataaa ttttttctcc
     9781 atttaggaaa aaggatgcta ggtcagcttg aaatatctta tcgtgtgtaa aagtgacatt
     9841 ctgtttcttc gctagttgat caatcaacat aaaaccaaca ttgtgttttg tttcaaaata
     9901 tttatcccct ggatttccca agcctacaag taatttggtc atttattttt cctttcaaaa
     9961 gccaaaaggg ttggaatttt ttttccaacc taattgacac catttattaa tttttagaca
    10021 ttaaagcgga attccatgat atcgccatct tgaacgatat attcttttcc ttcttcacgc
    10081 aagcgtccag cttcttttac ggccttttca gatccgtatt tcactagatc ttcatatgac
    10141 atggttactg cacgaataaa gcctttttca aagtctgagt ggataatacc agctgcttga
    10201 ggagccttca taccacgttt gaaagtccaa gcgcgaactt ctttttcacc agctgtgaag
    10261 taagttccca atccaagcaa gtggtaagct gcacgcgtca acttatctac acctgattct
    10321 gtcaaaccaa tggcttcaag aaactctttt ttatcttcat catccaattc agaaatttct
    10381 tcctcagcac gcgcagaaat aacgactact tcagcatttt ctgtcgctgc aaattcacga
    10441 atttgtttga catagtcgat agagtcaggt tctgaaacca catcctcgtc cacattagct
    10501 acataaagaa ctggtttagt cgtcaaaagg aaaagacctt tgacaacctt ttgttcctca
    10561 tctgtaaatt caatggtacg agctgatttc ccgtcttcta ggactggttt aatcttttga
    10621 agaacattga attctgctac tgattcttta tctttttgcg tacgtgccat cttttctaca
    10681 cgcgcatatc gtttgttcac tgattctaag tcagcaagaa tcaattccag attaattgta
    10741 tcaatatctg caagtggatc tacaaaggcg tcttcacgtc cttgctcgcg catcacattt
    10801 tcatcatcaa aagcacgaac tacgtgaaca atcgcatcta cttcacgaat attggccaag
    10861 aatttattcc ctagcccttc tccttttgaa gctcctttta caatccctgc aatatctgta
    10921 aattcaaatg ttgtgggaac tgtcttttta ggagttatca tttcagttag tttttgtagg
    10981 cgttcatctg gaacttccac cattccaaca tttggatcaa tagtcgcaaa tgggtagttt
    11041 gctgcctctg ctcctgcttt tgtaattgca ttaaataatg ttgatttacc aacgtttggc
    11101 aaaccaacga tacctgctgt caaagccata tttctcattc tccgttttca tttcaatccc
    11161 taatattata acacaaaaag ggataaagtg ctaaccctct aactaagggt tctcagtcaa
    11221 taattttatt catttttcgc tcaaaatcat atcgccccat catgacaaca tgctcacaat
    11281 tactgcattt tattttgata tctgctccta cacgtgtaat ttcccaacga ttagcctttt
    11341 taccagtcga cttgattgta caagcgtgtg attttttcat ctcaacaaaa tttccaactt
    11401 gatacatact actctccttt tctttttcga ttatatcata aaagaggcga gccaggctca
    11461 acctctttca cttaatttgt acgaactggt gtaatgagct gcatgaagtc ttcgtcagta
    11521 tctgctggca caagagtaaa tggacgaaca gctgagataa agctaatggt caccttttcg
    11581 ctatttaaag ctttaagaga atcaatcaag taagttgggt tgaaactaat ggtcaaatct
    11641 tcaccagtaa cctgatcagt atcgatttct tcgtttactt taccaacttc tggagagtga
    11701 acatgggcgc taacaacccc atccttaatt tcaagtttca cagtaccatt ttgagtcgca
    11761 cttgataaaa gacgggcacg ctccattgac tggcgtaagt ttaccacatt aaaagtaata
    11821 gtagtgttaa agtctgttgg aatcaagcga tctgtatcag gatagtttcc ttctaggaga
    11881 cgagtataga agctaatatt ttcgcttcta aagaggattt ggttattggc aaagaaaatc
    11941 tctacagttt cgatatcatc tgtaaatacc gctgaaaatt cgcgtagaga acggctagga
    12001 attacgacat caaaatcatc actatttttt tcaagagtca attttttctg gcttaggcga
    12061 tgagagtctg ttgcaactgt ttttaactct ttgtgttgac tcaatacgaa gtggacacct
    12121 gttaaaatcg gacgactctc ttgtgtactt gcagcaaagg ctgtttcatt aataattttc
    12181 ttgagtaatt ttgtttcaag tattaaagga gtgcttgctg aaatttcttg gattcgtgga
    12241 tattgttcgc tatcttttcc ttttagggta atttctgatt tgccactggt taaaacaatt
    12301 tgattttgtt caatttcttt aaaatcaaga gtcacatcag gtaaactaga tactacattg
    12361 ataaagaaag aagcttcaag aaggatcgaa cctaaagaag taattaacaa accagcatct
    12421 tcattttttt gagaaataaa attttcaatt gaaatttgac catttgaacc aattaaagta
    12481 ataccttcat tggtcacgtc aatttttact gttgataaaa taggaatggc atttttagaa
    12541 ctaatagctc tcttagtagt atttaatgct tgtagaaata aatttttatt aattgaaaaa
    12601 tgaatcatgg attctccttt atttattttt agtattagaa agataatagt aataatagag
    12661 tctgtgaaat ctgtggaaaa cagcctttaa acaagtcata tcaagttttt tagcttgttt
    12721 aaaaaatgtg gataaaaaag ataaaaaact aaaagttatc cacaaattat ttgattttct
    12781 ttttgattga ttcaatttct aaacgtaaat tatcgtcttg atcaatcaaa gattttattt
    12841 tggcatgggc atgaatgact gtggtatgat cttttccccc aaattccttc ccaatttttg
    12901 gaagactatt atctgttagt tctctagata aatacatggc tacttgacgg gccaaaacaa
    12961 tattttgaag gcgtctactt cccttcattt ctttgatact aacaccataa aagttaccaa
    13021 cttcagtttg gattttatca attgggatga cgagcatttg gctaacatct tgtttgcggg
    13081 ctctaatggc ttctgcagca atatcaatag tgatatcctt gatttttttt actctggcaa
    13141 ttaaagtgat gtcgttgatg gctccctcaa gatctcgaac atttgaatca aattgcccag
    13201 ctaggtattc tagagtatca ctttggaaat tgtagcctaa atgttccgtc ttactttgta
    13261 aaatggcaat acgtgtttca aagtcagggg gtgtgatagt ttgtgtcaat ccccaactaa
    13321 aacgcgtgac aagcctctcc tcgagccctt ctagatgttt tggactacga tcactcgtta
    13381 ggacaatctg tttttgcttg tcatgaaggg cgttaaaggt attgaaaaat tcttcctgag
    13441 ttgcgacttt ttttccgctg agtgactgta tatcatcgat taacaaaaga tcaagactac
    13501 gataggtctt tttaaacttt tccatttccc caagtcttag gtgatcaaga aagtcattaa
    13561 taaagctttc ggcagggata tatttaacac gcgcattagg aatatttttt agaatttcat
    13621 ttccaatagc gtttaataag tgagtcttac caaggcctgg tcctccatag ataaaaagag
    13681 ggttataggt cagagccaaa tcttcagaga cagctaaagc ggctgataca gcccaaacat
    13741 ttccatcccc ttgaataaag ttatcaaagg tatacttttc ttttaatccc gtatctgaat
    13801 aaggaataga tactaacttt ggactatagt cataaagagt taaatttgta gcttcttcaa
    13861 cttgtgagct agtcgtatct tgaggtttgg tgaaaatata gtggggagtt atttcagcgt
    13921 cataaatttc aaaaccagct actacaataa tatcttttag ttgtttttcc cagaccattt
    13981 ccatttcaga gcgaggtaga aatatagtgg caacattttc ctctaccttg atgagttcag
    14041 cttgaatagc atagaaatca tacatggatc gagtcagtct ttcttgtgca aattctaata
    14101 tacgattcca aaattgtttt tctttcaatc ctttctcctc ctttactatt taaaagttta
    14161 atactagaga tattttacca tagatagcaa gaattttcca caagctgtga aaaataatcc
    14221 acaatgttac caaactttat ccacaggttg gggataaaag aagaaattat tgatttatta
    14281 agctttttat cgaaaaacat agtggataac cttgtgagat aaaaaaataa aagaacagcc
    14341 ttatttcagg ctgttgataa ttctactata ttcttcttga tttgaaaaag aaataatgat
    14401 ttttccactg tcttttttag atagtttaat ttctacatct aatccgagta gttttcttaa
    14461 ctgtttttct tcattttgta tgaaatgatt agttttttgc tgtttctttt gttttttctc
    14521 tgtcagaaga gtttctaact tccttacaga aatatcttct tctataatcc gttgaaagaa
    14581 atagtcttgt tgttccttat ttaacccaac tagggaacgc gcatgggctt gtgatagttt
    14641 gccattttct acttctgaga gaatctgttc tggcaaggaa agtaaacgaa tggagttgct
    14701 gatatatgga cgagacttgc ccatcttatc tgcaatttca gcatgggtga atcctttctc
    14761 tacgagagat tcataggcgc gtgcttcttc tattgggttt aaattttctc tctgtaaatt
    14821 ttcaataatg gactggacca tcatctcttg gtctgaaatc tgtttaacaa cagctgggat
    14881 agaccgtaga ccagctaaaa gtgaagcccg atagcgtctc tctcctgcaa ggatttcata
    14941 accaataaca ggagattgac gaacaataat cggttgaatg accccatttt ctttgataga
    15001 ctgtgctagt tcatctagct tttctctatc aaattctttt cggggttgat agggattttt
    15061 ttgtatatct gtgatagaaa tcatttcaaa tttttccatg attctacact aacacatctt
    15121 ttctcttatg taaagctttc tttacataga tgtcaattaa gattctaaat cacctgaact
    15181 cttgttaagt ttgatagagg tagtttcttc tttcccgtta cgatagtagg ttatcttaat
    15241 ggtgtctccg atagaatggt tgtaaagagc actttgtaag tctgttgatg aagcaatctc
    15301 tttgtcatct acttttgtaa ttacatcgta tttttcaagg tgaccattgg caggcatatt
    15361 actttgtacc gaacgaacaa ctacaccaga tgtaacatta cttggaatat tgagtcttct
    15421 gatgtcgctt gtactcacat tagataaatt aaccatctgg attcccaaag ctggacgcgt
    15481 cacttttccg tttttttcta actgttcaat aatattgata gcatcatttg caggaattgc
    15541 gaaaccaaga ccttctacag atgttcctcc atttgtagca attttacttg aggtaattcc
    15601 gataacctgc ccttgaatat tgatcagtgg gccgccagag ttacctgggt taatagcagt
    15661 atcagtttgg atggcttttg tagaaatagc ttgtccatct tccgatttta aggatacatt
    15721 tctattgaga ctggatacga taccttgagt gacagtattt gcatattcag aacctaacgg
    15781 gctaccgatg gcaatagcag tttctcctac agttaactta ctagaatcac caaactcagc
    15841 tactgttgtc actttttctg aagagatttt tacgacagca atatcagaga aagtgtcagc
    15901 tccgacaatt tctccaggta ctttagtccc atctgacaat cgaatatcta ctttgctggc
    15961 gccatttata acgtgattgt tggtgacgat gtaagcttct ttatcattct ttttataaat
    16021 aactccagat ccttcactag agattcgctg agaatctgtg tcagtatcat cattgccaaa
    16081 tacgctattt tgtctgtttg ccgaataagt aataacagaa acaacagcat cttttacttt
    16141 gttaacagcc tgtgttgttg aattttcgtt cttataggca gtttgtgtaa tagtactatt
    16201 attgttagag ttgtttacac tacttttttg agttagttga gttattgaaa aactacccaa
    16261 ggctccacta aaaaagctaa tgacgataac gactaataat tgaaaccatt ttttgtaaaa
    16321 tgtttttaga tgtttcatat ttgcctccat atgtttgaat tactgaaagt ataaactgac
    16381 tagcttaatt ataacttaaa cacaaaagtt ttacacaaac tgtggataac tcttttgaaa
    16441 ctgtgatttt cttaattgaa atctattttt tattttgtga ataagatgtg aaaaaataga
    16501 gaatatgtta gaatagagtc atgaaaatta aagttgtaac agttgggaaa ctgaaagaaa
    16561 agtatttaaa agatggtatc gcagagtatt caaaacgaat ttctagattt gctaagtttg
    16621 aaatgattga gttatcagat gaaaaaacac cagataaggc cagtgaatca gaaaatcaaa
    16681 agattttaga aatagaaggt cagagaattt tatcaaaaat tgctgaccgt gatttcgtta
    16741 ttgtgttagc cattgaaggg aaaactttct tctcagaaga atttagtaag cagttagaag
    16801 aaacttctat aaaaggattt tctactctta cttttattat tgggggaagt ttaggattgt
    16861 catcatctgt aaaaaataga gccaatcttt ctgtcagttt tggtcgccta accttgcctc
    16921 atcagttaat gagactagtt cttgttgaac aaatctatcg cgcttttacg attcagcagg
    16981 gattccccta ccataaatag aaaattgact tttaattgaa tttttggtag aataattgtg
    17041 ttaggtctca tagctcagct ggatagagca ttcgccttct aagcgaacgg tcgcaggttc
    17101 gaatcctgct gggatcaata taatagcaaa gctgggaatt ttcccggctt ttttcttaaa
    17161 aaagtacact ttgggagaaa aaaatgacag ttgagagaat tttatctaaa gtgaaattcc
    17221 attttgtata atggtttttg taagttagct tacaagaaaa aacattttag gagattttat
    17281 tatgaaaaac acagttaaat tggaacagtt tgtagctttg aaggaaaaag acttacaaaa
    17341 tattaaaggt ggggagatga ggatttcaag aatcatcctt gattttcttt tcctacgaaa
    17401 aaagtaatga aataaggaga aagagtaatg gatttacttg gatttgggac agttattgtt
    17461 cattttttaa ttattagtca cagttaccgt ttaatttgta aaggtcgaat aaatagaaaa
    17521 gaattataca tttttggtgc ttatacatta ctaactgaaa tagtacttga attttccttt
    17581 tatcttctat atttagataa aatagggatt gaaagatttt tatttccttt gggcttatat
    17641 tcctattttc gatggatgaa acagtatgag agggatagag gactattcct aagtttacta
    17701 ctatctcttt tatatgagag cactcataac tttctgtccg taactttctc ctctataaca
    17761 ggagataatt ttgttttaca atatcatttc ccattctttt tcgttgtgac ggtgttaacc
    17821 tattttgtta cattaaaaat catttattat ttccatttgg aactagccta ttttgacaag
    17881 gactaccttt atcctttctt gaaaaaagta ttttttgctt tactattgct acatattgta
    17941 tctttcgttt cagatatggt aagtacgatt aaacatttga atagttttgg aagtattttg
    18001 tcatctattg tctttatctc tctccttttg accttctttg caatgaattc tcataaagtt
    18061 caaatggaga aagagattgc tttgaagcag aagaaatttg aacagaaaca tttacagaat
    18121 tacacagatg aaattgttgg tctgtataat gaaatccgtg gttttcgaca tgattatgct
    18181 ggaatgcttg tcagcatgca gatggcaatt gacagtggta atttacagga aattgacaga
    18241 atttacaatg aagttttagt caaagcaaat cataaattgc gttcagataa gtacacttac
    18301 tttgatttga acaacataga agactcagct ttacgaagtt tggttgctca gtcaattgtc
    18361 tatgctcgaa ataatggtgt agagtttaca ctggaagtaa aagatacgat taccaagctt
    18421 ccaattgaac tattggattt ggttcgtatc atgagcgttt tattgaataa tgctgtcgaa
    18481 ggatcggctg atagctataa aaagcagatg gaagtagcag ttattaagat ggaaactgaa
    18541 acagttatgg tgattcagaa ttcatgtaaa atgacgatga ctccttcagg agatctattt
    18601 gccttaggat tctccactaa gggaagaaat cgcggagtcg gattaaataa tgtgaaagaa
    18661 ctactagata agtacaacaa tattatttta gaaacagaga tggaaggcag tacatttaga
    18721 caaatcatta gatttaagag ggaatttgaa tgaaagtttt aattttagaa gatgttattg
    18781 aacatcaagt gagactagag agaatattgg atgaaatttc gaaagaatcg aatattccaa
    18841 tatcatacaa gacaacggga aaagtccgtg aatttgaaga atacattgaa aatgatgaag
    18901 taaatcagct ttatttccta gatatcgata ttcatggaat tgagaaaaag ggatttgaag
    18961 tggctcagct cattcgtcat tacaatcctt acgctattat tgtctttatc actagtcgat
    19021 cagagtttgc gactctaacc tataaatacc aggtatcagc cctagatttt gttgataagg
    19081 atatcaatga tgagatgttt aagaagagaa ttgagcaaaa tatcttctac acgaagagta
    19141 tgttacttga aaatgaagat gttgtagatt atttcgacta caattacaag ggaaatgatt
    19201 taaaaattcc ttaccatgat attttgtata ttgaaacaac aggggtatct cataaattgc
    19261 gcattattgg taagaatttt gcaaaagagt tttatggtac catgacagat attcaggaaa
    19321 aggacaaaca tactcagcga ttttattctc ctcacaagtc atttttggta aatataggca
    19381 atatcagaga aattgatcga aaaaacttag aaattgtttt ctatgaagac catcgttgtc
    19441 ctatttcaag attaaaaatt agaaaattaa aagatatttt agagaaaaaa tctcaaaagt
    19501 gattgacaat tagcaagaaa ttgatataat ggttatatct gctacagata gaaggtccgt
    19561 tggtcaaggg gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg
    19621 gactatttta tccgccatag ctcagttggt agtagcgcat gactgttaat catgatgtcg
    19681 taggttcgag tcctactggc ggagtataga taaaagggaa cacagctgtg ttcctctttt
    19741 tgtatcaatt tgtatcacca agcattttca taaggaagtc tgttatttct tgagaacttt
    19801 ctttttttcc atgtgcaatc caagtttggc agacaccaaa aagtgcatga gttagataga
    19861 tgctactata ttctaattca gtggtattta gattcagttg cataaatcgc ttttgtaaat
    19921 ctatactgag catgatatga agtttatttc gtaagaaatt ttggatttct ttagtcccat
    19981 tttcagaaag aagggcagcc agaagtggtt ctgactctag atattcaaag acttctaaaa
    20041 tagcgtctct tttgtgatga gcatgttttt gaaaaatata ttcaaatgta tggaatagct
    20101 tgctttgata gtgctcaatc acatcatact tatccttata gtgagtatag aagctggaac
    20161 gactaattcc ggctttttct actaatttga cagtagaaat tttatcaaat ggctgttcca
    20221 tcagtaattg taccatagca ttttcaatag ttcgctttgt ttttaagcgt ttgttacttt
    20281 cttgcatatt tcctccttgt aaacaaatta gactatatgt ctaaaaatag attttttatc
    20341 ttgtaattta gattttttaa tgtataatct attatattaa aattttagac aatatgttta
    20401 aaaaaggaga aactaagttt aaagaatgga aagcaattta aaaaaaacca acctttatta
    20461 ttgtcatgat cgggatttct cttattccag atctgtacaa tatcatattt ttgtcatcaa
    20521 tgtgggattc atatgggcaa ttgtctgact tacctgtggc agttgtaaat aatgataaag
    20581 aggcttccta taatggtaat actatggcaa taggaaaaga catggtgtcc aatttaaaag
    20641 aaaataaaac cttggatttt cattttgtag atgaagagga aggaaagaag ggattggaag
    20701 atggcgatta ctatatggta gtgactttac caagtgattt atctgaaaaa acaactacat
    20761 tatccaatat tcaatcgaca gcagcttatc aatcattgac aagtgagcaa caaactgaga
    20821 taagtgattc tgtatctcaa aattcaactg atagtattca atcggctcag tcaattgtag
    20881 ctttagtaca agatttacag ggaagtttag aaaacttaca aaatcaatct tctaatcttt
    20941 cgactttaaa aaatcaatct aatcaagtat cacctattac ttctacttct ttgataggat
    21001 tgtcaagtgg attaacagag atacaaggag atgttactag caaattagtt cctgccagtc
    21061 agtcgattgc atcaggtgta aacgcatata ctacaggtgt tgataaagtt tctcagggcg
    21121 caagtcaact aagtgaaaaa aatgccacct tgacaggtag tttggataaa ctagtttcag
    21181 gctcaaacac cttgacacaa aaatcttcta gattgacagc aggagttggt taattacaat
    21241 caggatctgg gcaattagca gacaaatcca gtcagttact ttcaggtgct tctccattag
    21301 agaatagagc taataaattg gcagatggat ctgggaaact agcagaaggt ggaacaaagt
    21361 taacttctgg attggaagat ttacagacag gacttgcttc tttaggacaa ggactaggta
    21421 atgctagtga tcaactcaaa tcagtatcaa cagaatctaa aaatgcagag attttgtcaa
    21481 atccactcaa tctttcaaaa acagacaatg atcaagttcc tgtaaatgga atcgcaatag
    21541 ctccttatat gatatcagtt gctctttttg ttgcagcaat atcaacaaat atgatatttg
    21601 cgaaattgcc ttcaggacgt catccagaga gccgttgggc ttggttgaaa tcttgagctg
    21661 aaataaatgg tattatagct gttttggcag gaattttggt atatggagga gttcagctta
    21721 ttggtttaac tgctaatcat gagatgagaa tatttattct catcatccta acaagtttag
    21781 tattcatgtc tatggtgacc actttagcaa cgtggaatag ccgtatagga gcttttttct
    21841 cacttatttt gcttttacta cagttagcat caagtgcagg tacttatcca cttgctttga
    21901 caaatgattt ctttagatct attaatccct ggttaccaat gagctattca gtttcgggat
    21961 tacgacaaac aatctctatc aacaagtcat tttcctagct gtcatactag ttctatttac
    22021 tagtttaggt atgctagcct atcaacataa gaaaatggaa gaagattaaa aaaatcgacc
    22081 gattaactgg tcgatttttt atgccttaga tgactttcgt ctgtgattat agattccaaa
    22141 tagtaagaga gaagtaaagg aacagattgc tccagtaata aaaccattgg gaatgaagga
    22201 aagtgtaata gttcctttcc ccttgggaat gtcaactttc ataaatccag tttgagcttg
    22261 tttaatttct attttcttac catcttggta ggcagaccaa cctttgtcat aaggaatggt
    22321 gaagaaaata gatgtatctt gttggacatc atatgtagca aaaaccttgt ttttagaagt
    22381 tgatactgtg acaggttgtt ctttaatttt ttgaattgcc tcggtgaaag ttttggtatc
    22441 taaacgatag aaggtaggag attcaaatga tacttgtgaa tttccaggga aactaacatt
    22501 gatattgaaa gtttttttct ctttagtata tcctagatta aagaaggaga agacattatc
    22561 agttgtaaaa gtcttttttt caccatttac aaggatgtca accttctttt gtttatcgtt
    22621 agaaaagtga aggtttatga aagagagata aacttggctg ttttctggaa cttcaatttg
    22681 atactggatt gctgcatctt catttgaaga acttgtgaca ctaatcaaat cattagtatt
    22741 ttctattttt tctgtttttt cataaggtat tggagaaaaa taatcaaaat tgacgttagc
    22801 aagttgattt aaaaatgagg cctgattatc caaggtatgt tcattgaact tgacatcatt
    22861 gtaaacagat tgactagcaa ctgcaatcgg aagagagtat tgattttcat atagggtaag
    22921 attatctttt tgatagatat ctttaaagcc atacttatca ataggactgt ctgagatatt
    22981 gtactggata ccaaataaac tatcagccaa aatactatta tttgcatatc ggagattgag
    23041 attagtccca gaggatttaa aaccaagttt atctaaagta gagcttgatg aacgatttcg
    23101 aacagatgaa aattgagaga ttccattgta gttgaatttc atactgtcat ttcctgtctg
    23161 agtttgtagt ttttcagtac gagtaaattg atttccaata tatgttgaga aagattccat
    23221 agctgggata tctcgactat aagcacttcg agaagcaaat ccccattcct tagcaattcc
    23281 gtccatttga gatgaagcat ttaaactcat ttcaaccagt ataaataaag agattagaat
    23341 ggcaaataga ttcacagata taaacttttt gataactgca aggagtaaaa gagaatagac
    23401 aaccaaaaat tcaagagtaa gcagaatatt caaatctgtt aaaaaagaat aatgcgattt
    23461 tagatagatg gtagctaaaa atcctgttac tacaagaaaa agcgaaacta aaaaattcca
    23521 gactttaagt tctttcagac gctttaagac ttctgctgct gtgtaaatta acaaggtaga
    23581 gaaaatccaa gcatagcgat gtaaaaacat gtttggagta tgcatgcctt gccaaaataa
    23641 gtcaagagct tctatgtaaa agcttgcaat tagaaatgca aagaatatta catatatgag
    23701 tttcacgtga aacttaatag atttcagcgt aaaaaataaa atggtcaaaa taaagggaaa
    23761 tagtccaaca aaaatcattg ggatggcccc atactttgtt gtgtcaaagg aaccaatgaa
    23821 ttgcttagca aagagatcaa gataccagct actttcagtt tgaaactttg taacttcagt
    23881 caatttttcc ccatgtgtct gtaaatcaaa tagagtggga agagtcataa tcaaactagc
    23941 cataccagct aaaaaggaga taactatgaa atcaagaaca gatgattttc gagtcttaaa
    24001 gtcccacgaa atttgacaga gataccagaa aataagaaac aatactgtca tatatccaaa
    24061 ataataattt tgaataaata agattgacag acttgtaaag tacaatagga gtttcttttc
    24121 agttatcagt agatgtaaac cagttataat taaaggaatc aagataaaaa catctagcca
    24181 ggtttttatc tctaattgac tgacagtgaa actcatcaga gcataggaag tagataaggc
    24241 tagttttaaa gtctgaggga tagattgaaa caatttattc aaactaaaaa aggttgacag
    24301 accaatcaat ccaaatttta agagagtggt cagatagaca gcatctggca tattcgttag
    24361 atcaaaaaag taaactagag gcgcgagaaa actacccaag taataactag atagggcata
    24421 gaagtttagc cctagaccac ttgtaaaggt gtaaaacaga ctactatttc catgtaggat
    24481 atttcgtaag gctacatcaa aaataacgta ttgatgaaag ccatctccta atagaggaga
    24541 gttgtcgcta ttccagtaga taccttgaga tagatatact ccagacataa tcactacagg
    24601 aatgatgaaa gaaataaaat aggttcgata tgtttttaaa aatgatttca tgttacctcg
    24661 tagaatgata gaaaactcag ttggttaacc caactgagtt ttgaagtttt atttagtctt
    24721 tccaaagttc tttaactttt gcttgtactt ctgcattttc taggaattca tcgtaggttt
    24781 catcgatacg gtcaatgacg ccatttttag ataagacaat gatatggtta gccaaagttt
    24841 gaataaactc gtggtcatgg ctggcaaaga tgattgattc tttaaagttt ttcaatccat
    24901 cattcaagct tgagatagat tccaagtcca agtgatttgt tggatcatca agtacaagga
    24961 catttgattt taagagcatg agttttgaaa gcatgacacg aactttttct ccccctgaca
    25021 agacatttac aggtttgtta acttcatctc cagagaagag catacggccg aggaaaccac
    25081 gtaggaaagt attgtcatca tcttctttac ttgcgaattg acgcaaccag tcaagaattg
    25141 attctcctcc tgcaaaatca gctgagttat cttttggtag gtaagattga ctagttgtaa
    25201 ctccccactt gacagttcct tcatagtcaa tatctcccat gattgcacga attaatgcag
    25261 tcgtttgaat atcattttgt ccaataagtg ctgtcttatc atctggacgc aagatgaaac
    25321 taatattatc caagatagtt tcaccatcaa tctttacagt tagattttct actgtcaaga
    25381 gatcattacc aatctcacgt tccgctttaa agttgataaa tggatattta cgactagatg
    25441 gtacaatctc ttctagctca atcttatcaa gcattttctt acgtgatgtt gcctgccttg
    25501 acttagaagc attggcagag aaacgagcaa cgaattcttg caattgttta attttttctt
    25561 ctgctttagc attacggtct gctagcaatt tagcagcaag ctcagaagat tccttccaga
    25621 agtcgtagtt tccgacatag agtttgattt ttccaaagtc aaggtcggcc atgtgagtac
    25681 aaactttgtt taagaagtga cggtcgtggg atactacgat aactgtgtta tcaaagtcaa
    25741 ttaagaagtc ttctaaccaa gtaatcgatt ggatatccaa accgttagta ggctcgtcca
    25801 agagaagaac atctggttta ccaaaaagtg ctttggcgag gagaaccttt actttttcac
    25861 cgttggccaa ttcgctcatg ttttggtagt gtaattcttc tggaatgttt aggttttgaa
    25921 gtagttgaga ggcttcactc tctgcttccc aacctccaag ttcggcaaac tctccttcga
    25981 gttcggcagc acgaaccccg tcctcgtctg agaaatcttc cttcatgtag atagcatctt
    26041 tctctttcat gatgctataa agtttttcat ttcccatgat aacgacatca atggcacgtt
    26101 catcttcgta gtcaaagtga ttttgacgaa gaacagagag acgttcatct ggaccaagag
    26161 agatgtgacc agtagtaggt tcgatatctc cagctaaaat ttttaaaaag gttgattttc
    26221 cggcaccatt agcaccgatt aatccgtaag tatttccttc tgtaaatttg atattgacat
    26281 catcaaaaag tttgcgatca ctaaaacgta gtgaaacatc agatactgta agcaatattt
    26341 ttctcctata tgtgtaatat atttattcta ctagaaaata cagaaatatt caaattttta
    26401 tttttcaatt ttgtgtaaat tatatttaca gtatccttta cacaaatctg taaaaagcaa
    26461 ggctgattta ttttgataaa ttacggttat ttcattaaaa aaatgctata attgaaagga
    26521 ctatatcgaa ggagaacaaa atgactaaac ccattatttt aacaggagac cgtccaacag
    26581 gaaaattgca tattggacat tatgttggaa gtctcaaaaa tcgagtatta ttacaggaag
    26641 aggataagta tgatatgttt gtgttcttgg ctgaccaaca agccttgaca gatcatgcca
    26701 aagatcctca aaccattgta gagtctatcg gaaatgtggc tttggattat cttgcagttg
    26761 gattggatcc aaataagtca actattttta ttcaaagcca gattccagag ttggctgagt
    26821 tgtctatgta ttatatgaat ctagtttcgt tagcacgttt ggagcgaaat ccaacagtca
    26881 agacagagat ttctcagaaa ggatttggag aaagcattcc gacaggattc ttggtctatc
    26941 caatcgctca agcagctgat atcacagctt tcaaggctaa ttatgttcct gttgggacag
    27001 atcagaaacc aatgattgag caaactcgtg aaattgttcg ttcttttaac aatgcatata
    27061 actgtgatgt cttggtagag ccggaaggta tttatccaga aaatgagaga gcagggcgtt
    27121 tgcctggttt agatggaaat gctaaaatgt ctaaatcact aaataatggt atttatttag
    27181 ctgatgatgc ggatactttg cgtaaaaaag taatgagtat gtatacagat ccagatcata
    27241 tccgcgttga ggatccaggt aagattgagg gaaatatggt tttccattat ctagatgttt
    27301 ttggtcgtcc agaagatgct caagaaattg ctgatatgaa agaacgttat caacgaggtg
    27361 gtcttggtga tgtgaagacc aagcgttatc tacttgaaat attagaacgt gaactgggtc
    27421 ctattcgtga gcgccgtatt gaatttgcta aggatatggg agaagtttat aatatgattc
    27481 aaaaaggtag tgaaagagcg cgtgaagttg cgggtcaaac cctatctgag gtaaaagggg
    27541 caatgggact tcattacttt aactaagttt attttacaag aaactatctt ttctatctcg
    27601 aagaaaagat agttttttat ttacccctgt aacatttgac aaatttttat agaatggtat
    27661 gatagataca atataaaaag agtcaagctc aaaaagaaag aaaagaggaa acttcgaatg
    27721 tctaattggg acactaaatt tttgaaaaaa ggttttacct ttgatgatgt attgcttatt
    27781 ccagctgaaa gtcatgtgtt gcctaacgat gcagatttaa caactaaatt ggcagataat
    27841 ttgactttaa atatcccaat tattaccgct gccatggaca cagttacaga gagtcaaatg
    27901 gccattgcta ttgctcgtgc aggcggtctc ggagttatcc ataaaaacat gtcaattgct
    27961 caacaagcag acgaggttcg taaggtaaaa cgttctgaaa atggagttat tattgatccg
    28021 ttcttcttga cgcctgaaca tacaattgct gaagcagatg agcttatggg tcgttaccgc
    28081 atcagtggtg ttccagttgt tgaaacactt gaaaatcgta aattggttgg tattttgaca
    28141 aaccgagatc ttcgttttat ttcagattat aatcaaccaa tttcaaacca tatgactagt
    28201 gaaaatcttg ttactgctcc tgtgggtacg gatcttgcaa cggctgagag tattcttcaa
    28261 gagcatcgta ttgaaaaact tccgttggtc gatgaagaag gcagtctttc tggtttgatc
    28321 actatcaaag atattgaaaa agttattgag tttccaaatg cggctaaaga tgagtttggt
    28381 cgtcttctag ttgcaggtgc agtaggtgtt acttcagata catttgaacg tgcagaggct
    28441 ctttttgagg caggagcgga tgcgattgtt attgatactg cacatggtca ttctgcaggt
    28501 gtcttgcgta aaattgccga gattcgtgct catttcccag atcggacttt gattgctgga
    28561 aatattgcta ctgctgaagg tgcacgtgcc ctttatgaag cgggtgtaga cgttgttaag
    28621 gttggtattg gaccaggttc tatctgtact actcgtgtga ttgctggtgt tggtgttccg
    28681 caagtaacag ctatctacga tgctgcagct gttgcgcgcg aatatggtaa aacgattatt
    28741 gctgacggtg ggatcaagta ttctggagat attgtaaaag cacttgctgc aggtggaaat
    28801 gctgttatgc ttggatctat gtttgctgga actgatgaag ctccaggcga aactgaaatc
    28861 ttccaaggac gtaaattcaa gacttaccgt ggtatgggat caattgctgc tatgaagaaa
    28921 ggttcaagcg accgttattt ccaaggttct gtcaatgaag caaacaagct tgttccagaa
    28981 ggaattgaag gtcgtgttgc ttataaagga gcggcagctg atattgtttt ccaaatgatt
    29041 ggtggtattc gctctggtat gggttactgt ggtgcagcta accttaaaga actacacgat
    29101 aatgctcaat ttattgaaat gtctggtgct ggtttgaaag aaagccatcc tcatgatgtg
    29161 caaattacta atgaggcacc aaattattct atgtaaaaaa caatgaaaag aactccagtg
    29221 aaaacaggag ttcttttaca atgttgtcaa tttccattta cagaagcttt accatcctga
    29281 atagtgaaga tacttagatt ttctggcaga ttttgaagat ggtctaagct tgttgttgtg
    29341 ataaaggttt ggattgattg agaaatcgtt tctaataatt ttaactgtct agtgttgtca
    29401 agttcactca tcacatcgtc aagcagtaat atcggagatt ctgtggtaat gctttccatt
    29461 aattcgattt ctgctaattt tatcgagagg acgagactac gatgttgacc ttggcttccg
    29521 aaactagcat ccatcccatt tatataaaaa gaaatgtcat ctcgatgagg accgacacca
    29581 gtattctttt taaataaatc tctggatcta cttttttcta aagcaatttt gaaagattcg
    29641 gataagtttt gtttgtcagt tatattgaca gaagattgat aggatattga caactcttcg
    29701 atctgattag agagttcaaa atgtttctta cgcccaaatg attctagttt ttttatgaaa
    29761 tctaagcggt gattcattac acgacatcca taatcaacta gctgatcatc taacacagaa
    29821 aggaatgttt catctatttt ttgatctgat tttaggtaag tgtttctttg ctttaggatg
    29881 tggttataat tggttaagtc agataagtag attggcttaa tttgcccaag ttccatatca
    29941 atgaattttc gtcgaatcga aggtgctcct ttaattagtt gtaaatcttc aggagcaaat
    30001 aagacaacat tcatgtgtcc tacataatct gaaaggcgtg cctgttttaa gtgattaact
    30061 tttgttacac ggcctttttg tgttagttcg atttctaggg gaatggatcc agtttttttc
    30121 tgaacgagac ctgaaagatg aagttgttcc tcatcaaaat gaatgagatt tttatctgtt
    30181 cgagttcgat gactacgcgt taaggctaaa aaatagatag cctctaacat gtttgttttt
    30241 ccttgtgcat tacgtcccaa aaagacattc aatttaggat taaagtctat tttcgtttct
    30301 ttgtaattac gaaaagtctt gagagataga tgttgtagcc acatgattat ctaccaggga
    30361 atcgtggagc ttgttggctt ttaggtgatg aagcaggttt cgatttgtct tttttaactc
    30421 ccttattcat ctttttgaca agtttagcga tccgttcttt ttcaacttta tcagcttggt
    30481 attcatcttg ttcttcagaa gtaggttgtg tcaacaagat gtcaatgttc atgtcaggga
    30541 tgtcaacttt atcgccaata cgaagttttt ttccacgacg actctctaat tccctattaa
    30601 agtaaacaga atgttcagag agaaatgatt taatagctcc tccgctatgt gtaattccaa
    30661 gttctttgag tagtgcttgg agggtaataa attcttcaaa taatttgtat tccataattc
    30721 acctcaatct ttggtattat accatatttt cctaaaaata gtgagagaga cagcgagaaa
    30781 tgtaagcgat ttttatttca aaagtgttac agggaacaag aaaatttcag gttttcgtga
    30841 tataatagaa gtctgtatat aaggaggtaa atcatggagt tagtgcatgg aatttcaaca
    30901 cattttatcc aatcaaaaaa gtttaaaaca aacaaaatta ccgtgcgttt taccgctcca
    30961 ttatcccttg atacgattgc aggtcacatg ttgagtgcaa gtatgctaga gactgctaat
    31021 cagatgtacc ccacttctca agatttgagg agacacttgg ccagtctata cggtacagat
    31081 atgtcaacca attgtttcag aagagggcaa agccacatta tagaattgac atttacctat
    31141 gttcgtgatg agtttttaag taggaaaaac gtgctaacct ctcagatttt ggaacttgta
    31201 aaagaaactc ttttttcacc cgtagtagtt gataatgggt ttgatccggc cttatttgaa
    31261 attgagaaaa aacaattgct agcaagttta gcagctgata tggatgattc tttttatttt
    31321 gcacataaag aattggataa attgtttttt catgatgaac gtcttcaatt ggaatatagt
    31381 gatttacgaa atcgtatttt agctgaaact ccacaaagtt cttattcttg tttccaagaa
    31441 tttttagcca atgatcgaat agatttcttt ttcctaggtg attttaatga ggttgaaatt
    31501 caaaatgtat tagaatcatt tggctttaaa ggtcgaaaag gagatgtgaa ggttcagtat
    31561 tgtcaacctt attctaatat ccttcaggaa ggtatggttc ggaaaaatgt gggacaatcc
    31621 attttggaat taggttatca ttactgttct aaatatggtg atgagcaaca tttacccatg
    31681 attgtaatga atggtttact tggtggattt gctcactcta agctctttac aaatgtccgt
    31741 gaaaatgctg gattagctta taccatttca agtgagcttg atttatttag tggattcttg
    31801 aggatgtatg ctggtatcaa tcgagaaaat cgtaaccagg ctcgtaaaat gatgaataat
    31861 caactgcttg atttaaaaaa aggttatttt acagagtttg agttaaatca gaccaaggaa
    31921 atgattcgtt ggtcgttgtt actttctcaa gataatcaat cttcattgat tgaacgtgct
    31981 tatcaaaatg ccttatttgg aaaatcttca gcagacttta aaagttggat tgcaaagctt
    32041 gaacagattg acaaagatgc tatttgtaga gtagctaata atgtgaaact acaagcgatt
    32101 tactttatgg aaggaataga atgacaaagg ttgtttttga agaaaaatac tatccagctg
    32161 taaaagaaaa ggtttatcga actcgtttgg ccaacggatt gacagttgct cttttgccta
    32221 aaaaggaatt taaagaggtt tacgggagtg taactgtaca gtttggttcg atagatacgc
    32281 ttgtcacaga agttgacgga gatgtaaaac aatatcctgg aggaattgct cattttcttg
    32341 aacataaatt atttgagaga gaagattcta gtgatttgat gtcggctttt acgagtctag
    32401 gtgcagatag taatgccttt acaagcttta caaaaacaaa ctatcttttt tcagcaacgg
    32461 attatttttt agaaaattta tatttacttg atgaattggt aacatcagca cactttactg
    32521 aagcttccat tctgacagag caggatatta ttcagcaaga acgagaaatg taccaagatg
    32581 atccagattc gtgtttattc ttttcaactt tagcgaattt gtatcctggt acacctttag
    32641 caactgatat agttggaagt gaggagtcca tttcccaaat caatctaact aatttgcaag
    32701 aaaattttac aaagttttac aaacctgtaa acatgtctct gtttttagtt ggtaattttg
    32761 atgtggagcg agtacaggac tattttgaaa gcaaagaact gaaagattca gattttcagg
    32821 aagtagcaag agaaaagttg tttttacagc ctgtaaagcc aacagatagt atgagaatgg
    32881 aagtatcttc tcccaaacta gcgattggag ttagaggtaa gcgagaagtt tctgaagcgg
    32941 attgctatcg acatcatatt ttattaaaat tattgtttgc aatgatgttt ggttggactt
    33001 cggatcgttt tcaaaaatgt tatgaatcag gtaaaattga tgcgtcctta tctctggaag
    33061 ttgaaataac aagtcgcttt cattttgtca tgttgacaat ggatacgaaa gagccagttg
    33121 ctttgtctca tcaatttagg aaggctattc gtaattttac aaaggattta gatattacag
    33181 aggaacattt agatattatc aaaagagaga tgtttggcga atttttcagt agcatgaact
    33241 ctcttgaatt tattgcaacg caatatgatg cttttgaaaa tggtgagaca atttttgatt
    33301 tgccgaaaat tttacaggaa attactttag aggatgtcct tgatgctgga catcatttaa
    33361 tagatgatgg tgacatagtt gattttacaa tattcccatc gtagtaacct attataatag
    33421 acactagaaa gaagggatga caagtatgag aaaaaaaaca attggagagg ttttacgatt
    33481 agctagaatc aatcagggat tgagtttaga tgaattgcag aaaaagacag aaatccagtt
    33541 agatatgttg gaagcaatgg aagcagacga tttcgatcaa cttccaagtc ctttttacac
    33601 gcgttctttc ttgaaaaaat atgcatgggc tgttgagtta gatgaccaaa ttgttttgga
    33661 tgcttatgat tctgggagta tgattactta tgaggaagta gatgttgatg aagatgagtt
    33721 gacaggtcgt agacgttcaa gtaagaaaaa gaagaaaaaa atatcatttt tacctttatt
    33781 ttattttatc ttttttgctt tatcgatttt aatttttgtg acttattatg tttggaacta
    33841 tattcaaact caaccagagg agccttctct ttctaattac agtgtggttc aatcaacaag
    33901 ttcaactagc tctgttcccc actcctcaag tagtagttct tctagtatag aatcagctat
    33961 aagtgtatca ggcgaaggaa atcatgtaga aatcgcttat aagacaagta aggaaacagt
    34021 taaattgcaa ttggcagttt cagatgttac aagttgggtc agtgtttcag aaagcgaact
    34081 tgagggcggt gtaaccttat cgccaaagaa gaaaagtgca gaagcaacag ttgcaactaa
    34141 aagtcctgta acaattacgt taggtgttgt aaaaggtgtt gatttgacag tagataatca
    34201 gactgttgat ttatcgaaat taacagctca gactggacaa atcactgtaa cctttactaa
    34261 aaattaagga aaaacgaatg aaaaaagaac aaattcccaa tctcttaaca ataggtcgaa
    34321 ttctctttat acctattttt atctttattt taacgatagg aaattcgata gagagtcata
    34381 tagttgcagc tattatcttt gctgttgcca gtattaccga ctatttagat ggatatttag
    34441 ctcgtaaatg gaatgtggtc agtaattttg gtaaatttgc agatcctatg gcggataagt
    34501 tactagttat gtcggctttt attatgttga ttgagttagg tatggctccg gcttggattg
    34561 ttgcagtgat tatctgtcgt gagttagctg tgacaggttt aaggctttta ttggttgaaa
    34621 ctggtggaac aattttagca gcagcaatgc ctggaaaaat taaaactttt agtcagatgt
    34681 ttgccattat tttcttgcta ttacattgga ctttgcttgg tcaagttcta ctttatgtag
    34741 ccttattttt cactatctac tctggctatg actatttcaa gggtagtgcc tatgtattta
    34801 aagggacatt tggttcgaaa tgaaatcaat aattgatgta aaaaatcttt cttttcgcta
    34861 taaagaaaat cagaactact acgatgtgaa ggatattacg tttcacgtga aacgtggaga
    34921 atggctttcg attgtagggc ataatggtag tggtaaatca acgacggttc gattaattga
    34981 tggcttactg gaagcagaat ccggagagat tgtaattgat ggccaacgtt tgactgagga
    35041 aaatgtttgg aatatacgtc gtcaaatcgg tatggttttt caaaatccag acaatcaatt
    35101 tgttggagcg actgttgaag atgatgttgc ttttggtttg gaaaatcagg gactttctcg
    35161 tcaagaaatg aaaaagagag tggaagaagc tctggcttta gttggcatgt tggactttaa
    35221 aaagagagag ccagcgcgtc tatcaggtgg ccaaaagcaa cgtgtagcca ttgcaggtgt
    35281 tgtagcccta agaccagcta ttttaatctt agatgaagca acgagtatgt tggatcctga
    35341 ggggcgtaga gaacttattg ggacagtaaa aggaattcga aaagactatg atatgacggt
    35401 catttctatt acccatgatt tggaagaagt cgccatgagt gatcgcgtat tggtcatgaa
    35461 aaaaggggaa attgaatcaa ctagtagtcc aagggagctt ttctctcgaa atgatttaga
    35521 tcaaattgga ttagacgatc cttttgctaa tcaattaaaa aaatctttga gccagaatgg
    35581 ctatgattta cctgaaaatt atttgacaga aagtgagcta gaggataagc tatgggaatt
    35641 gctctagaaa atgtgaattt tacatatcaa gaaggtactc ccttagcttc agcagctttg
    35701 tcggatgttt ctttgacgat tgaagatggc tcttatacag ctttaattgg gcacacaggt
    35761 agtggtaaat caactatttt acaactctta aatggtttat tggtgccaag tcaagggagt
    35821 gtgagggttt ttgatacctt aatcacctcg acttctaaaa ataaagatat tcgtcaaatt
    35881 agaaaacagg ttggcttggt atttcagttt gctgaaaatc agatttttga agaaacggtt
    35941 ttgaaggacg ttgcttttgg accgcaaaat tttggagttt ctgaagaaga tgctgtgaag
    36001 actgcgcgtg agaaactggc tctggttgga attgatgaat cactttttga tcgtagtccg
    36061 tttgagctgt cagggggaca aatgagacgt gttgccattg caggcatact tgccatggag
    36121 ccagctatat tagtcttaga tgagccaaca gctggtctag atcctctagg gagaaaagag
    36181 ttgatgaccc tgttcaaaaa actccaccag tcagggatga ccatcgtctt ggtaacgcat
    36241 ttgatggatg atgttgctga atatgcgaat caagtctatg taatggaaaa gggacgttta
    36301 gtaaaggggg gcaaaccaag tgatgtcttt caagacgttg tttttatgga agaagttcag
    36361 ttgggagtac ctaaaattac ggccttttgt aaacgattgg ctgatagagg cgtgtcattt
    36421 aaacgattac cgattaagat agaggagttc aaggagtcgc taaatggata gtatgatttt
    36481 agggcgttat atcccagggg attcgattgt tcaccgattg gatccacgta gcaaattgct
    36541 tgctatgatg ctactgattt tgattgtgtt ttgggctaat aaccccttga cgaatctgat
    36601 tctttttata gcgacaggga tatttattgc cttgtcagga gtatctcttt cattttttat
    36661 tcagggcttg aagtctatgt ttttcttgat tgccttcaca actatttttc aactattttt
    36721 catttctaat gggaatgttt tatttgagtt ttcgtttgtg agaatcacgg attatgcttt
    36781 gcaacaagct gggattattt tttgtcgctt tgtattgatt attttctttt caactttgtt
    36841 aaccttaaca accatgcctt taagtttggc atcagctgtc gaagctttat tagcaccttt
    36901 aaagcgtgtg aaagttccag ttcatgaaat tggattgatg ctgtctatga gtttgcgttt
    36961 tgtcccaacc ttgatggatg atacgactcg gattatgaat gcacagaaag ctcgtggagt
    37021 ggattttgga gaaggaagca tcgttcaaaa agtaaaggcg atgattccca ttttgattcc
    37081 tctttttgcg acaagtttaa aacgtgcaga ttccttggct atcgctatgg aagcgcgtgg
    37141 ctatcagggt ggaaaaggca gaagtcaata ccgacaattg aaatggactc tgaaggatac
    37201 gctgaccatt cttgttattc tcgtacttgg ttgttgttta tttttcttaa aatcttagta
    37261 gctttcagga attgataaaa agttactgta acagtttttg atataaaggt aggaatatga
    37321 accgttttaa aaaatcaaaa tatgtcatta ttgtttttgt cactgttctg cttgtgtcag
    37381 ctctcttagc gacgacttat tcaagtacaa ttgtgacaaa attaggagat ggaatctcat
    37441 tggttgatag agttgtacaa aaaccttttc agtggtttga ttctgtcaaa tcagatttgg
    37501 ctcatttgac acgaacatat aatgaaaatg aaagtttgaa gaaacagctt taccaattag
    37561 aagttaaatc aaatgaggtg gaaagtttaa agacagaaaa tgaacaactg cgccaattgc
    37621 ttgatatgaa gtctaaattg caagccacaa agactttagc agcagatgtt attatgcgtt
    37681 ctccggtatc ttggaagcag gagttgacct tagatgcagg tagatcaaaa ggtgcttctg
    37741 agaacatgtt agctattgca aatggtggct tgattgggag tgtttcaaaa gtagaggaga
    37801 actctactat agtcaacctt ctgacaaata cggaaaatgc tgataagatt tctgttaaaa
    37861 ttcaacatgg ctctactaca atttatggaa ttattattgg ctatgacaag gaaaatgacg
    37921 ttcttaaaat tagccaatta aatagtaata gcgatattag tgcgggagat aaggtgacta
    37981 ctggtggatt aggaaacttt aacgttgctg atattcctgt tggtgaagtg gttgccacaa
    38041 cgcatagtac agactatttg acacgagaag taactgttaa attgagtgca gatactcata
    38101 atgtagatgt gatagaatta gtggggaatt cataatgaga cagttgaagc gagttggagt
    38161 atttttattg cttcctttct ttgttctaat tgacgcccat attagccagc ttctgggctc
    38221 atttttcccc catgtacatt tggctagtca ttttcttttt ctatttctct tatttgagac
    38281 gatagaagta tcagagtatc tctacctagt ctattgtttt gttataggct tggtttatga
    38341 tgtttacttt ttccatctaa tagggattac aactctctta tttatcttat tgggagcctt
    38401 ccttcataaa ttgaatagtg ttattttgtt gaatcgttgg acaagaatgc tagctatgat
    38461 tgtgctgaca ttcctgtttg aaatgggtag ttatcttttg gcttttatgg tagggttgac
    38521 agtagatagc atgtcgattt ttatagtcta tagcttggta ccgacgatga ttttaaattt
    38581 tttatggatt actgtttttc aatttatttt tgaaaaatat tatctataag aacgacatat
    38641 aaatgtaaca aaggcgtaat atttattagg cctttttttg gtatactagt attgtcttta
    38701 aaagaaggag tatctacgta atatgaagaa aaaaatctta gcgtcacttt tattaagtac
    38761 agtaatggtt tctcaagtag ctgttttaac aactgcgcat gcagaaacga ctgatgacaa
    38821 aattgctgct caagataata aaattagtaa cttaacagca caacaacaag aagcccaaaa
    38881 acaagttgac caaattcagg agcaagtatc agctattcaa gctgagcagt ctaacttgca
    38941 agctgaaaat gatagattac aagcagaatc taagaaactc gagggtgaga ttacagaact
    39001 ttctaaaaac attgtttctc gtaaccaatc gttggaaaaa caagctcgta gtgctcaaac
    39061 aaatggagcc gtaactagct atatcaatac cattgtaaac tcaaaatcaa ttacagaagc
    39121 tatttcacgt gttgctgcaa tgagtgaaat cgtatctgca aacaacaaaa tgttagaaca
    39181 acaaaaggca gataaaaaag ctatttctga aaaacaagta gcaaataatg atgctatcaa
    39241 tactgtaatt gctaatcaac aaaaattggc tgatgatgct caagcattga ctacgaaaca
    39301 ggcagaacta aaagctgctg aattaagtct tgctgctgag aaagcgacag ctgaagggga
    39361 aaaagcaagt ctattagagc aaaaagcagc agctgaggca gaggctcgtg cagctgcggt
    39421 agcagaagca gcttataaag aaaaacgagc tagccaacaa caatcagtac ttgcttcagc
    39481 aaacactaac ttaacagctc aagtgcaagc agtatctgaa tctgcagcag cacctgtccg
    39541 tgcaaaagtt cgtccaacat acagtacaaa cgcttcaagt tatccaattg gagaatgtac
    39601 atggggagta aaaacattgg caccttgggc tggagactac tggggtaatg gagcacagtg
    39661 ggctacaagt gcagcagcag caggtttccg tacaggttca acacctcaag ttggagcaat
    39721 tgcatgttgg aatgatggtg gatatggtca cgtagcggtt gttacagctg ttgaatcaac
    39781 aacacgtatc caagtatcag aatcaaatta tgcaggtaat cgtacaattg gaaatcaccg
    39841 tggatggttc aatccaacaa caacttctga aggttttgtt acatatattt atgcagatta
    39901 atttacagag ggactcgaat agagccctct tttcaggttt taccgtgaca atccctatta
    39961 aaaattatat caaaatagct tgaaaatatt ggaaaagtat ggtagaatga aaattgtcgt
    40021 gtgaacgata atactcattc ttgatgaatt gtgaagcagt tgcccttggg tcgttttgcg
    40081 agttgaagtc aagaagagga aaaaaacaaa aaggagaaat actcatggca gtaatttcaa
    40141 tgaaacaact tcttgaggct ggtgtacact ttggtcacca aactcgtcgc tggaatccta
    40201 agatggctaa gtacatcttt actgaacgta acggaatcca cgttatcgac ttgcaacaaa
    40261 ctgtaaaata cgctgaccaa gcatacgact tcatgcgtga tgcagcagct aacgatgcag
    40321 ttgtattgtt cgttggtact aagaaacaag cagctgatgc agttgctgaa gaagcagtac
    40381 gttcaggtca atacttcatc aaccaccgtt ggttgggtgg aactcttaca aactggggaa
    40441 caatccaaaa acgtatcgct cgtttgaaag aaattaaacg tatggaagaa gatggaactt
    40501 tcgaagttct tcctaagaaa gaagttgcac ttcttaacaa acaacgtgcg cgtcttgaaa
    40561 aattcttggg cggtatcgaa gatatgcctc gtatcccaga tgtgatgtac gtagttgacc
    40621 cacataaaga gcaaatcgct gttaaagaag ctaaaaaatt gggaatccca gttgtagcga
    40681 tggttgacac caatactgat ccagatgata tcgatgtaat catcccagct aacgatgacg
    40741 ctatccgtgc tgttaaattg atcacagcta aattggctga cgctattatc gaaggacgtc
    40801 aaggtgagga tgcagtagca gttgaagcag aatttgcagc ttcagaaact caagcagatt
    40861 caattgaaga aatcgttgaa gttgtagaag gcgacaacgc ttaatttata caaatagtaa
    40921 ttacctagga gggcggggct tagcccggct ctcctatttt caaaaaatat aggagaatta
    40981 aaatggcaga aattacagct aaacttgtaa aagagttgcg tgaaaaatct ggtgccggtg
    41041 ttatggacgc taaaaaagcg cttgtagaaa cagacggtga catcgaaaaa gcgattgaat
    41101 tgcttcgtga aaaaggtatg gctaaggcag ctaagaaagc tgaccgtgtt gctgcagaag
    41161 gtttgactgg tgtttatgtt aacggtaatg ttgcagcagt tattgaagta aacgctgaaa
    41221 ctgacttcgt tgcaaaaaac gctcaattcg ttgaattggt aaatactaca gctaaagtca
    41281 ttgctgaagg aaaacctgct aacaacgaag aagctcttgc tttgataatg ccttcaggtg
    41341 aaactcttga agctgcatac gtatctgcaa cagcaactat cggagagaaa atctcattcc
    41401 gtcgctttgc attgattgaa aaaacagacg cacaacactt tggagcatac caacataacg
    41461 gtggacgtat cggtgttatt tcagttgttg aaggtggaga cgaagcactt gctaaacaat
    41521 tgtcaatgca catcgcagcg atgaaaccaa cagttctttc ttacaaagaa ttggatgagc
    41581 aattcgttaa agatgagttg gcacaattga atcacgttat cgaccaagac aacgaaagcc
    41641 gtgcaatggt taataaacca gctcttccac acttgaagta tggatcaaaa gctcaattaa
    41701 ctgatgatgt tattgctcaa gctgaagctg acatcaaagc tgaattggct gcagaaggta
    41761 aaccagaaaa aatctgggac aaaattattc caggtaaaat ggatcgcttc atgcttgata
    41821 acactaaagt tgaccaagct tacacacttc ttgcacaagt ttacatcatg gatgacagca
    41881 agacagttga agcatacctt gaatcagtta acgcttcggt agttgagttt gctcgctttg
    41941 aagttggtga aggtatcgag aaagctgcaa acgacttcga agctgaagtt gcagctacaa
    42001 tggcagcagc cttgaataac taataataga aaggaagcaa attgcttcct tttttgtata
    42061 gggcttagat ttttcctgaa attgtttttt ccccagcctc actcgattag aataattaaa
    42121 acaaaaagcc ctataataag ggctaagtgc atttaggaga ctatacttca aattcatagt
    42181 aagcgcgtca taacaaggta tctatcctaa ttccccaact tccagtatac tagataaaaa
    42241 ataattatcg gaggaaaggt attgtactat gataattcaa ctaagtgatt taggtcaagt
    42301 tcaccttgtt tgtgacaaga cagatatgag gtagggaata gactcattag cctatgtagt
    42361 taaaacccac tttgaattgg attctttctc cggtcaagtc tttctctttt gtggtgaacg
    42421 taaagaccgc tttaaagccc tttactggga tggtcaagga ttttggctac tatataaacg
    42481 ctttgagaac ggaaaactga cttggctaag tacagaaaag gatgtcaaag ctctcgcacc
    42541 tgaacaagta ga
//
LOCUS       DBEJAQ010000013        40037 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.013, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000013 DBEJAQ010000000
VERSION     DBEJAQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 40037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.013"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(181..1359)
                     /locus_tag="ACL297_000750"
     CDS             complement(181..1359)
                     /locus_tag="ACL297_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGR0071041.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLISQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            1639..2901
                     /locus_tag="ACL297_000751"
     CDS             1639..2901
                     /locus_tag="ACL297_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGR0071042.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            2904..3800
                     /locus_tag="ACL297_000752"
     CDS             2904..3800
                     /locus_tag="ACL297_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HGR0071043.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            3815..4132
                     /locus_tag="ACL297_000753"
     CDS             3815..4132
                     /locus_tag="ACL297_000753"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0071044.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            4149..4466
                     /locus_tag="ACL297_000754"
     CDS             4149..4466
                     /locus_tag="ACL297_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGR0071045.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            4476..5831
                     /locus_tag="ACL297_000755"
     CDS             4476..5831
                     /locus_tag="ACL297_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGR0071046.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLNQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWHAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            6154..7629
                     /locus_tag="ACL297_000756"
     CDS             6154..7629
                     /locus_tag="ACL297_000756"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HGR0071047.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVNFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            7626..8474
                     /locus_tag="ACL297_000757"
     CDS             7626..8474
                     /locus_tag="ACL297_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HGR0071048.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEDPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTGLDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            8489..9259
                     /locus_tag="ACL297_000758"
     CDS             8489..9259
                     /locus_tag="ACL297_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGR0071049.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            10062..10301
                     /locus_tag="ACL297_000759"
     CDS             10062..10301
                     /locus_tag="ACL297_000759"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGR0071050.1"
                     /translation="MNERYQCLKTKEYQALLSSKGRQIFAKRKIDMKSVFGQIKVCLG
                     YKRCHLRGKRQVRIDMGFILMANNLLKYNKRKRQN"
     gene            complement(10411..10614)
                     /locus_tag="ACL297_000760"
     CDS             complement(10411..10614)
                     /locus_tag="ACL297_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGR0071051.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(10645..11253)
                     /locus_tag="ACL297_000761"
     CDS             complement(10645..11253)
                     /locus_tag="ACL297_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGR0071052.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(11292..11462)
                     /locus_tag="ACL297_000762"
     CDS             complement(11292..11462)
                     /locus_tag="ACL297_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HGR0071053.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(11474..12040)
                     /gene="amaP"
                     /locus_tag="ACL297_000763"
     CDS             complement(11474..12040)
                     /gene="amaP"
                     /locus_tag="ACL297_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HGR0071054.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(12124..12288)
                     /locus_tag="ACL297_000764"
     CDS             complement(12124..12288)
                     /locus_tag="ACL297_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGR0071055.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            12731..14212
                     /gene="mgaSpn"
                     /locus_tag="ACL297_000765"
                     /gene_synonym="mgrA"
     CDS             12731..14212
                     /gene="mgaSpn"
                     /locus_tag="ACL297_000765"
                     /gene_synonym="mgrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor transcriptional regulator
                     MgaSpn"
                     /protein_id="HGR0071056.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            14574..15575
                     /locus_tag="ACL297_000766"
     CDS             14574..15575
                     /locus_tag="ACL297_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGR0071057.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     KVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            15726..16643
                     /locus_tag="ACL297_000767"
     CDS             15726..16643
                     /locus_tag="ACL297_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071058.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAVRLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            16654..17544
                     /locus_tag="ACL297_000768"
     CDS             16654..17544
                     /locus_tag="ACL297_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGR0071059.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            17573..19189
                     /locus_tag="ACL297_000769"
     CDS             17573..19189
                     /locus_tag="ACL297_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0071060.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            19199..20518
                     /locus_tag="ACL297_000770"
     CDS             19199..20518
                     /locus_tag="ACL297_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGR0071061.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            21041..21409
                     /locus_tag="ACL297_000771"
     CDS             21041..21409
                     /locus_tag="ACL297_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071062.1"
                     /translation="MRLSKEARVVLEELKLEYLKKGEDKTSGVIVDEIFKHFSSNFQK
                     VDWKSVQSAPKFNEILADYTSVNPTTLNLSNSTISTMSYIRDHFNYILDMKRTVYRSF
                     IIRLVLKAYKLEKEGTDIYL"
     gene            21522..21764
                     /locus_tag="ACL297_000772"
     CDS             21522..21764
                     /locus_tag="ACL297_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071063.1"
                     /translation="MKINEYLWRTSCLILLIIMSFFAGFGAKLEFTSYNPFLSVKFGL
                     VVALTVFVVQLLAILPAMVKRGNKNENVESNNSCEE"
     gene            21727..23118
                     /locus_tag="ACL297_000773"
     CDS             21727..23118
                     /locus_tag="ACL297_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HGR0071064.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNIKIVRLFIQIFVFLLLISSWIA
                     IIPFIARLVFSMFFISLLFFVEKFFRSNFIAKLRNKLNIREALFYMLISLNLYDELDN
                     EVVDTAVLYFDVENNKVVVCAPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKIV
                     YVLGQKEEIEQYVFNSNTLTREFFKDIPSSIIKLSNTQKFSLKSNTNLGIYGRTGTGK
                     TIALQWYLFNALAKGCGIADNTYLGIVDGKAADLYRIGELLHEELGEQVAVGSSPQML
                     AQLSRKFIENMDARFKIIKQNSSLNADIYELDELGLVPNFLFVDELASIRDSCGSTKQ
                     GRETWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGAPGAD
                     RLKMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKKEFLNVVRN
                     ILPTNDGLPPLAK"
     gene            23374..23793
                     /locus_tag="ACL297_000774"
     CDS             23374..23793
                     /locus_tag="ACL297_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071065.1"
                     /translation="MVYNMTDTNIKIGLLDLEMSQFDIPKKVVILSVRVNNKYKEVNG
                     ENVKTDEVSKITSAVLDADKVKVLTEMGISTDDLKAINLEIIGNLDKIAKLAQNDALL
                     NVPVELIRPKVRLAWNMARNNWSGVKIVCEDIKVLGE"
     gene            23798..24817
                     /locus_tag="ACL297_000775"
     CDS             23798..24817
                     /locus_tag="ACL297_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HGR0071066.1"
                     /translation="MNTVRIDYFAVTVKNISPEKVLTDILLIPLENFTLNDWGINKYK
                     QHYSCSEIKVYFNADRLSMGVFIELKGQGCRQYEEFLNSNENNWIALISRLYHHNANF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSVLDSGERVGETVAIGSR
                     GNQQWCIYNKRMEQLGKQELVEVSSWTRAELRCWQEKANIIAEQLYLKRPLSSIYFEA
                     INGHYRFIRPNAIDSNRWRRKKVEWWVNYLGTENQTVLSITRAKTTLRESEVWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQNLLKEGLSKLTDNDEKDIEQYIREQNSSQLWGT
                     DGRSD"
     gene            24821..25003
                     /locus_tag="ACL297_000776"
     CDS             24821..25003
                     /locus_tag="ACL297_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HGR0071067.1"
                     /translation="MADTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            25005..26132
                     /locus_tag="ACL297_000777"
     CDS             25005..26132
                     /locus_tag="ACL297_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGR0071068.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSCIRASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHAESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDELNEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELN
                     IDNENNLIFQHFGYVYDVPDIATVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNN
                     IDLGVIAKILGHKDISMLIDVYGHTLEEKISEEFMAVKSLL"
     gene            complement(26215..26287)
                     /locus_tag="ACL297_000778"
     tRNA            complement(26215..26287)
                     /locus_tag="ACL297_000778"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26252..26254),aa:Lys,seq:ctt)
     gene            complement(26552..27823)
                     /locus_tag="ACL297_000779"
     CDS             complement(26552..27823)
                     /locus_tag="ACL297_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGR0071069.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            28093..28293
                     /locus_tag="ACL297_000780"
     CDS             28093..28293
                     /locus_tag="ACL297_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071070.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            28497..28676
                     /locus_tag="ACL297_000781"
     CDS             28497..28676
                     /locus_tag="ACL297_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071071.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            28813..28992
                     /locus_tag="ACL297_000782"
     CDS             28813..28992
                     /locus_tag="ACL297_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGR0071072.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            29029..29481
                     /locus_tag="ACL297_000783"
     CDS             29029..29481
                     /locus_tag="ACL297_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGR0071073.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            29772..30242
                     /locus_tag="ACL297_000784"
     CDS             29772..30242
                     /locus_tag="ACL297_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HGR0071074.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            30261..31349
                     /locus_tag="ACL297_000785"
     CDS             30261..31349
                     /locus_tag="ACL297_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGR0071075.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            31330..31758
                     /locus_tag="ACL297_000786"
     CDS             31330..31758
                     /locus_tag="ACL297_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGR0071076.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            31896..32846
                     /gene="prmA"
                     /locus_tag="ACL297_000787"
     CDS             31896..32846
                     /gene="prmA"
                     /locus_tag="ACL297_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGR0071077.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            32848..33591
                     /locus_tag="ACL297_000788"
     CDS             32848..33591
                     /locus_tag="ACL297_000788"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGR0071078.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDFIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            33602..35398
                     /gene="pepF"
                     /locus_tag="ACL297_000789"
     CDS             33602..35398
                     /gene="pepF"
                     /locus_tag="ACL297_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGR0071079.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(35458..36135)
                     /locus_tag="ACL297_000790"
     CDS             complement(35458..36135)
                     /locus_tag="ACL297_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGR0071080.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQKKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEQGKEF
                     LEKEWGI"
     gene            36382..37152
                     /locus_tag="ACL297_000791"
     CDS             36382..37152
                     /locus_tag="ACL297_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071081.1"
                     /translation="MLYLLGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDFLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
     gene            37145..37909
                     /locus_tag="ACL297_000792"
     CDS             37145..37909
                     /locus_tag="ACL297_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071082.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            38334..39125
                     /locus_tag="ACL297_000793"
     CDS             38334..39125
                     /locus_tag="ACL297_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HGR0071083.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLVFTSF
                     AKEIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSILFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            39236..39904
                     /locus_tag="ACL297_000794"
     CDS             39236..39904
                     /locus_tag="ACL297_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGR0071084.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFANKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLIIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT        12366 a         6449 c         8326 g        12896 t
ORIGIN      
        1 gatcgattcg tcctgttatt atttcatttt actatacatt atgaaaaagg ggagatagga
       61 gtataattcc aaatataaag aaagcgaaca aaacagaatt ctgattttca gaaaacaggt
      121 ttgttcgctt tttatgatat actccctctt tttagcctat ttattaaatg taatagaaaa
      181 ttaggaatgt agaattgtat gatacaaaca aagtgcaata gctgagtgtg cggctgtaaa
      241 atcattgtaa ggttcaataa tgacttcagg taggcgatta tagagcagtt tgggtttgta
      301 gttcatttca ctatatagtt tttggatgat gtgttgattt gttagtaaag gactatgtag
      361 gtagattttt tgagagtcaa tcatcatact gatatttaaa atggtttgac taagatagag
      421 taaggcttga tggataagtg tgattattcc agtgtcacct aattgataag cggttaaaat
      481 aacttggata tcaatatcat cggcattttt gactaaacta ggaagtagag aatagggaga
      541 ttgatgatat agaatttttg attttttgat taaccaagat tctccagcaa aagtttgtaa
      601 gcatcccttg cgcccgcaac tacattcttc tccctcagat gaaacaactg tatgccctat
      661 ttctccaatc attagatttc cttttccata aatgtttccg tcgtagatat aagaacagtg
      721 catacctcta gcaaaatgaa aataagcaaa attagggtca ttctgttggc gactaaatag
      781 gcgtttacct atagccatac agttaacatt gttagagaaa aacaaaggct tatcaaagtg
      841 ggattgaatc atttctaagt cgatatgttg ccataaagga ttgtttgttg ttattttata
      901 atcatctaaa tagcgaccag gtagcgcgat tccaattgct tctatttcat aatcagagca
      961 gttatttaag aattgcttta gtgtctggct aatgagttga tttccctttt cttggattaa
     1021 ctgtttagta acaatttctt tctcttcctt taagatattt cctagattat ctcctaaagc
     1081 aaaagtgaaa tgtttttcag atagttcaca accaatgtag taaaatcgtt tagcttgaat
     1141 gtctagcagg attttttttc tccctacgct tgtatcgtgt tcgtcttcac caagttctaa
     1201 gagaatgttt tctttaatca gatcatttgt gatactgcta gttgttgcag gtgttattcc
     1261 tgttttagta gcgatttcaa ttcgtgaaat gggacctaat gtgtaaactg tttccagtat
     1321 tttagctcgt agttgtaatt gtttcttaga taaggtcata ttagttttct tcctagttgt
     1381 cagattctta aaatcctatg attttttgct atggataaat gtagatgtta atctttttaa
     1441 gcttttcatt gcttactggt ctctttttat tatatattga taataaaaaa taatcaaata
     1501 tttacacaaa tccttgacaa ggctttctta tagttgtaaa atataaataa caaaagataa
     1561 tcaaataaca acttgacgtc aaaaaataaa gataattgat aacttttgat aataaaatat
     1621 atttaagagg aaaaggttat ggaaaaatta ttacaagaaa agttacttcc agtagcggct
     1681 agattgggta ataataaagc gttggtttct attcgagacg gtattactct aactatacct
     1741 ttattgttga taggttcatt actaatggtt attgcaagtt ttcctattcc aggatgggaa
     1801 aaatatctgg gtgatattgg tgttgctgac tatttatgga agggtgttga tagtagtttt
     1861 ggattacttg gtcttgtagc tagttttggt atcgcttact ttatggctag acaatacaaa
     1921 gttgatggta ttcctgctgg tattgtgtca ttatcatctt ttattacagt tacgcctttc
     1981 attagaggag aggctggagc aggaatgcct accgctttca tggcatcaaa aggtttattt
     2041 gttgctatga ttttaggatt gattaatggc tatatctacc aatggtttat aaatcataat
     2101 atacagataa aaatgccgga tggtgttcca ccagcagtat ctaaaagctt tagtgccatt
     2161 attcctggtg cagtgactat tgttggttgg ttgattgttt atgcaacttt agataaatta
     2221 agtttaccta atcttcatga aattgctcaa gtagctttgg gaggtccact tggactttta
     2281 ggaaataatg ttattggtct tcttatctta attttcctta atagtagttt ttggtttgta
     2341 ggattacacg gaggaaatgt tgttaatgca gttatgaaac cgttatggtt agctaatcta
     2401 gatgcaaata aagtagctta tcaaactgga gaaactcttc caaatatttt tactagcgta
     2461 tttatggata attttgtatt cataggtggc ggtggtgcaa ccataggttt agttcttgcg
     2521 ttaggatatt tagcacataa gaagaaagct agtaaacaac taaaaacttt ggcacctata
     2581 actgttatac ctggattatt taatataaat gaaccagcaa tgtttggcgt tcctattgtt
     2641 ttgaacattt tgttactagt tccatttatc ttagctccaa tgtttaactt actagtagcg
     2701 tggggagcaa tggcatcagg tttggttccg ctaacttata cagatccagg ttggactatg
     2761 ccacctgtta taagtggttt acttgctaca ggaagtattt ctggttcatt attacaaatt
     2821 gtattgattg ttttggatgt cttactttat ttaccatttg taatagctat tgaaaaacgg
     2881 tttaagttat tggaggattg agaatgaaac gtttaattag tgcaaatcca tctgagatat
     2941 tacagatgaa tgctgaagaa ttaaaacaaa gtattttagc aagtgaaggt agagttgttc
     3001 tatctgaaaa tgtagttact cgtgagacat ttgttgggga tataactaat tctgaaattg
     3061 ctagagcttt tggagctgat atgattttat tgaattgtgt tgatgttttt gagcctaaaa
     3121 tttatgcttt ggatagttca ggtgatgatg ttattcatcg cttacaccag cttgttgctt
     3181 gtccaattgg tgtaaatttg gaaccgattg acccatctgc aaagatgcta gaggaaacac
     3241 aggaaattgt tgcaggtcgt gttgctagtg ttgaaacatt gaatcgaata gaggagttag
     3301 gttttgactt tgtctgtttg actggaaatc ctggaacagg agttagcaat cgagaaatca
     3361 ttaaggctgt tcaaactgct aaggaaaact tttctggttt gattattgca ggtaagatgc
     3421 acggggcagg agtgaatgag cctgtggcag agctttctgt agcagagcaa ttgttggaag
     3481 caggtgcgga tgtgatactt gttccagcag ttggaaccgt tccagccttt catgaccaag
     3541 agttgcgtga agtcgttgat ctcgttcata gtaagggtgg gctagtactg agtgctattg
     3601 gtactagcca agaaacatct gatacagata ctatcaagga aattgcactt agaaataaaa
     3661 tttgtggagt tgatattcaa catataggtg acgcaggata tgggggactg gcaacagtcg
     3721 ataatattta tgcattgagc aaggcaatta gaggagtgag acatacagta tctcgcttgg
     3781 ctaggtcagt aaataggtga taaaggagta agctatggct aaagtaacaa ttatgttagc
     3841 atgtgcagca ggtatgagta caagtctgct agtgacaaag atgcaaaagg cagcagaaga
     3901 taaggggttg gatgcagaaa tttttgcagt tccagctcct gaagcagaag aaattgtagc
     3961 aacaaaggaa gtaaatgtgt tgcttttagg ccctcaagtt cgctatttac taggggactt
     4021 tcaagaaaaa ctaaaagata gacagattcc tgtggcggtt attccgatga cagattatgg
     4081 aatgatgaat ggttctaaag tcttagattt agctgaaagt ttattagact aagattgagg
     4141 agaatgctat gaatgaaagt aatttagaat ctgcaatggg gctaattatg tatggtgggg
     4201 aagccaaaag taatgctatg gaggctattc aggcagcaaa aaaaggggat ttctctaaag
     4261 ccaatcgaag attagctgat gcgaatgctg ccttattaca ggcgcataag gctcaaacag
     4321 aaatgttgac aagagaggca cagggggaag aaacttcaat tagcctcttg atggtacacg
     4381 cgcaagatca tttaatgacg agtctgacct ttgttgactt ggcgaaagaa gtggtagaag
     4441 tatacgaacg atttgaaaaa aattaggaat ttgctatgaa tacaatgttg gataaaatgc
     4501 aagaaaaact ttctccaatt gcaatgaaag ttgggaatca gaagttttta gttgctttac
     4561 gtgattcatt tgtggggact atgcctgtta ttatgacagg ttccattgct ttgttattaa
     4621 atgcttttct agtggattta ccacaacaat ttcacctaga aagtattacg aaaacctttc
     4681 agtggcttgt tgacatcaat aatttggtat tcaagggaag tataccaatt gtttccttat
     4741 tgttcatcta ttgcttggga gtgaatatcg ctaagattta caaggttgat acagtttctg
     4801 ctggtttggt atcacttgct tcgtttgtca tttcgattgg aagtacagtt acaaagagtt
     4861 tccctttggc aaatgtagga gatgtgaaat taaatcaaat cttacaagga attgataatc
     4921 tagcatttga tggtaaaaat ctgatggtaa ctattgggaa tgtgattcca ggaaaccata
     4981 ttaatgccag aggatacttt acagccatga tgattggatt tttagcctct attattttct
     5041 gtaaagtaat gaaaaagaac tgggtaatca agttaccaga ttcagttccc cctgcgattg
     5101 ctaagccgtt cacttctatc attccaggat ttatggcgat gtatatagta gccatcttga
     5161 cttatgtttt ccatttactt agcaatgact tattgattga ttgggtttac aaggtgttac
     5221 aaacgccatt actaggtttg tctcaaagtt tctttgcagt tattctgatg atctttttga
     5281 ataagttatt ctggtttttt ggtctccatg gaggaaatgt attggctccc atcatggaag
     5341 ggctatttgg agttgctatg ttagcgaatc tggatgcttt ccaaaaaggt gaaccgattc
     5401 cttatatatg gacaagtggg tcttttgggg cgtttgtctg gtttggagga ttaggattag
     5461 tacttgctat tttaattttt tcaagaaaca gtcattatcg aaaagttgct aaacttggtc
     5521 tagcaccagt tctgtttaat attggtgagc cggtcaatta tggtttacca gttgtcttga
     5581 atcctttgct atttatacca tttgtactta gcccggtttt catggcaacg gtagcttact
     5641 gggcaacaag ctggggtctt gtttcacctg ttacacaaaa tgttacttgg gtaatgcctc
     5701 caattttata tggtttcttc tctacagcat ttgattggca tgccatcatc ttatcagttg
     5761 tttgtttgat tattagtgtc ttgacttatt tcccatttgt gaaaatggca gataaaactg
     5821 aattgagtta atggtcttac ttgcgttttt tgtgataaaa tagaaataga ttacgtgtaa
     5881 gatgagttag gtgtaggatt tcttatctcc actaaaattg ttaatagaca aaggtttata
     5941 gtttattgaa gttggaatag tacactgtaa cttctaaaac agtactagaa aatgatttga
     6001 atttcctaat cgatttagtc atattttagt tcatttttat ataaaacata acaagaatgt
     6061 tattgtttta tatagagaga ttgatataga aaggggtgca gtaaaggtag atatcttctt
     6121 gtgcctcttt tgccatatta gaaaggagat tttatggttc agacaaaaca gccaaatatt
     6181 attttgattg ttgtagatca aatgagagca gacgccttat ccttgaatag taaggataag
     6241 ctagttagca ctccaacatt agatatgatg gctagtgtag gttataattt tgaaaatgcc
     6301 tattctccag ttccttcttg cgttcctgct agggcagctt tattgactgg cttagaccaa
     6361 gataaaagtg gacgtgttgg ttatcaagat gaggtgcctt ggaattttac taatactctt
     6421 ccaaaagtct ttaaagatat gggttatcaa acagagtgta ttggtaagat gcatgttttt
     6481 ccttccagac agagacttgg gtttgatcat gtcttacttc acgatgggta tctacatgtt
     6541 gatagaaagt atgataaggc ttatggaagt caatttgatt atgctagtga ctatctagcc
     6601 tttttaaagg gtaaagttgg ttatgatgtt gatttgattg atgacggaat ggattgtaat
     6661 tcttgggaag ctagaccttg ggataaagat gaaaagttac atccaacaaa ctgggttgtg
     6721 agtgagtcca tttcattttt acaaagaaga gatccaacgg ttcctttttt tctcaaaatg
     6781 tcttttgaaa aaccacatgc tccattaaat cccccaaaat actattttga tatgtatatg
     6841 gaacgcttgc ctcaatttct cgatttgcat atcgggaatt gggaagtatt agaaaagcaa
     6901 attccgtcta tttatgcttt aagagggaaa ttaaaggaag atgatcaaag aagaatggtt
     6961 gctgcttatt ttggattgat tactcatata gaccatcaaa ttagtcgctt tttgactgcc
     7021 ctaaaagaat ttcgtcatga taaagatacg attatctggt ttgtttcgga tcatggagat
     7081 cagcttggtg aacattatct gtttagaaag ggatatcctt atcagggaag tatccacatt
     7141 ccttcgttta tttacgatcc ggctggattg atagctggta ataggggaac aatcaagcaa
     7201 ttagtgaaaa ttcaagatat ttttccttca ctcgtggatt tggcaggagg aactactaca
     7261 gatgaactag atggacggag tgttaagaat ttattatttg gacagtacga ggggtggcga
     7321 acagaatttc atggtgaaca tgctttagga aaagattcta gtcaatatat tttgacagac
     7381 cagtggaaat ttatctggtt tcctgtttta aatcattatc agttatttga tatgaaaaaa
     7441 gatccgcatg aaatgaatga tttgtatcca agtgaaaaat accaacctat tgttcgtcag
     7501 atgaagaaaa aattagtaaa ctttcttaga tatagagaag aaggttttgt tgtggatgaa
     7561 gagttagttc ctgttgaact ttctaaaata acgccaactc taacgaaaac gggggatagt
     7621 caatcatgaa aatgaaaaaa atcaagggtg ggacatttat gatgggaaca aattctgaag
     7681 agggcttttt agatgatttt gaagatcctc aagtcgctgt tagtgtaaag gatttttcca
     7741 ttgcggatac tccagttacg aatcaagaat ttgctcaatt tgtgaaagaa acaggctata
     7801 aaacactagc tgagcgacaa gagtggtctt ttgtttttat attgttcgtt ccggaggcag
     7861 aaagggaggg atatcctcat cctgccgggg ctccgtggtg gttacaagtt ccgaacgctt
     7921 gctggaaaca tccctacggt gagaacagta atctagtggg tttagaagac catccagtag
     7981 ttcatgttgc tttagaagat gcattggctt tctgtaattg gagcggaatg tctttaccga
     8041 ctgaagcaca gtgggaatac gctgctagag gaggaagaca atctgaatat ccttggggag
     8101 atactctttt agaaggtgga tattatcatg ccaatacttg gcaaggaaga tttccttatg
     8161 aaaatactgg cttagatgga tttataggaa cggctcctgt ctatgaattt ttacctaatg
     8221 attttggtct atatcaaatg attgggaatg tttgggaatg gtgtcgcaat ccgagatata
     8281 ccttattagc tagttttaat gaagatgact atgagttgcc aaaatatggg atacaagatg
     8341 aggagtatgc tatcagagga ggttcctttt tatgtcattg ttcttattgt aatcgatacc
     8401 gagtagctgc tagaaatgga tgtatttcta cttccactag tagccatcta ggttttagat
     8461 gtttgaaaga ataggatgga cttaaaaagt ggttgagatt atttattttc taatcattat
     8521 aatagctagt ggattgggtt ctatatcagg catgggtggt ggaattataa ttaagccact
     8581 tatggatagt tttggatacc attcggtcag tgatattgct ttttattcta gtttctctgt
     8641 ttttataatg gcgattattt caacaaccaa aagattttca caaagtaaag agataaaatg
     8701 gagattgatt tttacggtat ccttttcttc tgttttagga ggatttctag gtcatcttat
     8761 ttttcaagtt ttgttatctc aattatcagt acggctagta tccattgttc aaatgatttt
     8821 actatttgtg atgctacttg tatcatttgt tttaacagat tttaaaaaaa cttatcaatt
     8881 tgataagata ggattctata tgatttgtgg tcttctattg ggcttgattt ctagtttttt
     8941 agggattggt ggaggtccgc tcaatgtatc tttattgatg gtgttttttt ctatttcgat
     9001 aaaagaagca acgatgtatt ctcttgcaat tattttcttt tcccagctgt ctcatttggc
     9061 tacaattgtt gtggttacag gtttaaatca gtatcattta gcacctgttc cagttatttt
     9121 tcttgcttct atatgcggag gggtattggg gacggttgta tcaaaagtct tacctgaaaa
     9181 ttgggtaaga tactgtttta aagggatgtt attctttgtc atgggaatga ctttatataa
     9241 tttatttcat attttgtaag gtcagtgtga aaatatttaa atagacttcc tgtaaaaact
     9301 ggatttaacg ccctccattt gtcaaattaa gctagacagt cttaccaact cagaaaataa
     9361 cctgatgagg tatttggtat ccaaaggatt taggtgggat gtgatttctt gaggctggga
     9421 aaaagtcttt aaaattaaaa aatgcataat atcaggtgtt ggaaaaactt tgatactatg
     9481 cgttttattg tgggaatatt tatttcattt tctcctgaaa ttgagttctt gcccagccta
     9541 aaattcttga ctgacttcgt tgaaatctgt ctgctttggc aagtcatgac accttaaaag
     9601 tctatttgaa tgttcgttga gacaccgcta tcttggacat cctagattcg caaaaaagct
     9661 ctcactatcg tgatgattgg aaatatcttt ccattttgaa aactcttttc cacagtcaaa
     9721 cgtaatagat ttaaagaggt tcactagaaa ttgtgccagc cacccttgga tggattctgc
     9781 catatccttt gccttacgac tcttggtctt caaactgaca atcaattgac tatacctgtt
     9841 cgctcacaaa taggagagaa ctttttacga aaatctaatg aatatgccac aagaacatta
     9901 taccgcaacg tgtactattt ttggttcatt ttactataga aactattgaa gcacctgtac
     9961 gaaaatatat catgaaaaag gggagataga aagcaaacag ccttgaaatc aatgttgact
    10021 gcgattatga acctgaatca gcccctcaaa agggactgta tatgaatgaa cgttaccagt
    10081 gtttaaaaac taaagaatat caggcacttt tatcttccaa gggtagacaa attttcgcta
    10141 aacgtaagat tgatatgaaa tctgtctttg ggcagataaa ggtttgtttg ggttataaga
    10201 gatgtcatct gagaggtaag cgtcaagtga gaattgacat gggattcata ctcatggcca
    10261 acaacctgct gaaatataat aagagaaaga ggcaaaatta aaaagctaga gttccgcaat
    10321 tgggaatctc tagctttttg ttttctgaga atcatcttga ctcagactct tttgactaat
    10381 gttactaaaa agataaagta acttttaacc ttatttatcg tcgccactca acatgttttt
    10441 aacaccttct acagcacctt ctacagcgtc tttggcatct tcggcgactt cttttacttt
    10501 agaaacaact ttttcagctg ctccttcttt ttccattttt tcatcaccga tggctttccc
    10561 aacaccttct ttaatagaac ctttagcttg atttaatttt tcttctactg acatgataat
    10621 ttctcctgtt atatttattt tatcttagtt tacacgagtg ttttcgtgag atactacacc
    10681 atttgctgca cctttaacag cttctacacc ttcgctaact ttttcttgaa cagttgagaa
    10741 accagatcca agaccagatt tagctttttc gaattgttct gaagtgaatt ctcctgttga
    10801 ttcagcaacg tcagatacgc gatcttgaag gcttactgag tctgcttcat gctgttcttt
    10861 agttttgatg tcgacaacgt ttacgttgat ttcaacaatt tccaagtcag tcattttagc
    10921 aacttctgaa gatacgattt ctctgatttc tgaatataaa gctggaacat ttttttggta
    10981 ctcaacaata acgtttaagt caactgcaac ttgtgtttta ccaacttcta cgttaacacc
    11041 acttgttacg tcatcgctgt taacgatttt ttctttaaga tttgagaaga aaccaccatc
    11101 gattcccaaa agacctgaaa cgttttctag tgaaagacca atgatttttt ggataacttt
    11161 atcttcgtaa gtaagttccc ctttgatttc gtgagctaca acagtagcat ctttcttttc
    11221 tacgttagtg tttgtgtttt tttcgtttga catatgaaac tcctttaatt aaattagtaa
    11281 tttttatttt tttatttatc tatatagttt ttttcgatat ataatccagc tgcaactccc
    11341 agtgctccta aaatcaatac aaatattgtt ttgaagaagc caaaggagac aatcaaacaa
    11401 gccagaaata cgcctatgag accagcgata attggatatc gatattgttt aagccattcc
    11461 attttttact tccttacttc acacgactaa cagtcttttt gttttgaggt tttggttggt
    11521 aattttttac tgcaacttca agttttactt gacgctcaat accaaaaaac tgcttcaatc
    11581 cattagttat ttcattttga attatttggc atctgtcagc gatgttgtct gaaggaagaa
    11641 ttttaccttc tacatgaacg aaacatttat tttttcgtaa atttacatga acagttgggt
    11701 tcttgatcaa tctatgatca ctcaccaaac ttctaacaaa accttcgatt gccgaattct
    11761 ttaattttaa tgtatcgttt ttagtttcaa gttggatttc caagtaacgt ttaggataaa
    11821 acatcacaac taaaatggat aataaaacta aagttgatag aaccactgtc ccccaaaaga
    11881 catatctagc aagatagaat tcaactacgg agttctgtct ccagctaagt agatgaatac
    11941 ctagatcact aacttgatga taatctatca aaacaggaag gaaaattgtc aagattaaaa
    12001 tacagaaaat aatgaaacat attttctttg cttttgacat atacaatcct ttcgatttaa
    12061 catttaaact atttttcccg tcattgattt aaaaatagtt tgctaacttt actggcgaaa
    12121 agtttacttt ctacctgtaa agaatgacac tacagtaatc acaatcgctg cacctacaat
    12181 agatgggagc aaagccattc cagcgattga tggaccccaa ctacctaaaa gagattgtcc
    12241 tgcatatgcc ccgactaaac cagcgaatac atttgcgatg attcccatag aactactttt
    12301 ttagtgattt tacctgccaa gagaccaata agacctccag caataattga tcacaacact
    12361 gcctactctc cttttttatt ttaatatcaa aaaatcaatt tctttgatta atactattat
    12421 ataactttaa tatagaaaag tcaattattt cgattgttat tattatataa ttttaaaaaa
    12481 tcaaattctt taattgttgc tattatataa tttttaaaat taaaaatata atctttagac
    12541 ttgaatttga ttctgtattc acgccctcaa tagacggtaa aataagaaaa ttgtttatat
    12601 attgtctccg tagtgttatt atacgaaata aaagatttta ggaaaagtcg ttatatcatg
    12661 ctatacctat tctttgtggt ataattgcaa gaggtttaat ccgataattt ataaagagag
    12721 aaagatattc atgagagatt tattatctaa aaaaagtcat aggcaattag aattattaga
    12781 attattattt gaacataaac gttggtttca tcgttctgaa ctagcagagt tattaaattg
    12841 tacagaacgt gcagtcaaag atgatctatc ccatgttaaa tctgcttttc ctgacttgat
    12901 ttttcattct tctactaatg gtatacgcat tattaatacc gatgatagtg atattgaaat
    12961 ggtttaccat catttcttta aacattcaac tcatttttcg attttagaat tcatcttctt
    13021 taatgaaggt tgtcaagctg agagtatttg taaagaattt tatatcagtt catcttcgct
    13081 ctatcgtatt attagccaaa tcaataaagt gattaaaagg caatttcaat ttgaagtcag
    13141 tctgacccct gttcaaatca ttggaaatga gagagacatt cgttactttt ttgcacaata
    13201 tttttcagaa aaatattatt tcctagaatg gccatttgaa aatttttcat cagagccact
    13261 atctcaattg ttagaattgg tttataagga aacaagcttt ccaatgaatt tgtcaacgca
    13321 tagaatgcta aagttgctcc tagttacgaa cctatataga ataaagtttg gtcattttat
    13381 ggaagtagat aaagattctt ttaacgacca aagtttggat tttttaatgc aggcagaagg
    13441 aatagaaggt gttgctcaga gttttgaatc agaatacaat atctctttag atgaagaagt
    13501 tgtttgccaa ttatttgtgt cttactttca aaaaatgttt ttcatagatg aaagtctctt
    13561 tatgaaatgc gtaaaaaagg atagttatgt tgaaaaatct taccatctat tgagtgattt
    13621 tattgatcag atttcagtca agtatcagat tgaaatggag aataaggata atctgatttg
    13681 gcatctgcat aataccgcac atctgtatcg tcaagagttg tttactgagt ttattttatt
    13741 tgatcaaaaa gggaatacaa tcaggaactt tcaaaatatt tttccaaaat ttgtttcaga
    13801 tataaaaaaa gagctttctc actatttaga gactcttgag gtttgttcta gttcaatgat
    13861 ggtaaatcat ctatcttata ccttcatcac tcataccaaa catttggtga ttaatttgct
    13921 acaaaatcag cctaaactaa aggttttagt tatgagtaat tttgatcagt atcatgcaaa
    13981 attcgttgca gagacacttt cttattactg tagcaataat tttgaactgg aagtttggac
    14041 tgaattagaa ttatcaaagg aatctttaga agattcacct tatgatatca ttatttctaa
    14101 ttttattatt cctcctattg aaaataagag actcatctat tcaaataata taaacacggt
    14161 ctcactcata tacttgttaa atgccatgat gtttattcga ttagatgagt aaccaaaaat
    14221 aacaaaaaaa cgctatttcg cgtttgaact gtacctcaaa agttagacag aaaaaatcta
    14281 atttttgggg tgtttttgtt aagaaaagac cttcaatcat ttaagcaaaa tgtacatcta
    14341 gatttatagc cctctaaaac tcttgctttt tttgtagaag atacgaaaaa agccataaaa
    14401 ccaaggcttt tcctgtttta tgtagatgtt tacacatgaa aattgaagta ttttcaaaag
    14461 ctaattggag tgattttatt catgatggga taaatgatct ggccaaaaat gattgagaaa
    14521 tagttgctta aaaagcattt gcatgatact atatattgaa aggaggtgat aatatggtag
    14581 aacaaagaaa atcaattacc atgaaagatg ttgctttaga agcaggagtt agtgttggaa
    14641 ctgtttcacg tgtaattaat aaagaaaaag gcattaaaga agtaactttg aaaaaagtgg
    14701 aacaagcgat taaaactttg aattacattc cagattacta tgctagagga atgaaaaaaa
    14761 atcgaacaga aacgattgca atcattgtac caagtatctg gcatcccttc ttttcagaat
    14821 ttgctatgca tgtggaaaat gaagtctata agagaaataa caaattactc ttatgttcta
    14881 taaatggtac aaatagagag caagactatc tggagatgtt gcgtcataat aaagttgatg
    14941 gagtggttgc cattacctat agtccaattg aacattactt gacgtcagga attccctttg
    15001 ttagtattga ccgcacatac tcagatattg ccattccttg tgtttcatca gataatgatg
    15061 caggtggaag agaagcagct aaacaattaa ttagtaaagg atgtcagcat ttagcctttg
    15121 tgggcggaca taatacaacc attaatgaaa ccaaacgtcg tagaatttca tttgaaaagt
    15181 atgtccaaga gcataaaata cctcttagta tttttgattt ggatgagaaa gttactgact
    15241 attatggaaa gctagagagt ttcttggaag aaaattcttc tatagatgga atttttacaa
    15301 tcaacgattt tacagctttg gatgtgattg aaatactaga aaaaaatggc aaacgtattc
    15361 ctcatgatgt ccaaattatt ggatacgatg gaattaaaat ggctggagat agagattatc
    15421 tactttcaac tattgaacaa ccattggaag aaatggcaaa agaagcggtt cgtattttgt
    15481 ttgatataat tgatggaaag actgttaatt tgcagacaat cctaccagta aaatttgttg
    15541 aaggaaaaac aacaaaaaat gaaaataagt cttgacagaa agcgctatca atgatagaat
    15601 gaattcagat aaaaagattt atttttaaaa caaaaatgaa acgtttcaaa aaaagaaata
    15661 aaaagacagc gccaagcgct atcttttata gaaaaaatga aacgtttcaa aaaaaggagg
    15721 ttgctatgaa tagcaaagcg aagcaagttt ctctttggga aagaatcaag aaacaaaaac
    15781 tcttgttatt gatgactgtc cccggtttag ttttaacctt tatctttaaa tacatcccta
    15841 tgtatggggt tttaatcgca tttaaagatt acaatccttt aaaaggaatt ttagggagtg
    15901 attggattgg tttttctgag tttacaaaat tcatatcctc tcccaacttt ggtatcttgt
    15961 tagccaacac attaaaatta agtatctatg gtttattgct tggcttttta ccaccaatca
    16021 ttcttgcgat tatgctcaat caactcttga gtgaaaaagt caaaaaacga attcagctca
    16081 ttttatacgc accaaacttt atctcagtcg ttgttattgt cggtatgatt ttcctcttct
    16141 tttcagtggg aggaccaatc aacaattttc tttctatgtt tggaatgaag gctgacttct
    16201 tgacaaatcc agacttcttt agacccttat acatctttag tggtatctgg caaggaatgg
    16261 gctgggcttc aacgctctac acggcaacat tggtaaatgt agatccagcc ttagtagaag
    16321 cagttcgact ggatggagcc aatatcttcc aacgaatctg gcacattgat attccagctc
    16381 ttaagcctat tatggttatc caatttgttt tagctgcagg tggaattatg aatgtcggat
    16441 atgaaaaagc attcttgatg cagacatcgt taaatttgcc aacttctgaa attatctcga
    16501 catatgtcta taaagttggt cttgtatcag gagactattc ttactcaaca gcggttggtt
    16561 tgtttaatgc agtgattaac gtagtattgc ttgttgcagt taaccaaatc gttaaacgca
    16621 tgaataatgg tgaaggaatt taaggaggaa agtatgaaaa attcgattat ggatacaaaa
    16681 tttgatagac gtatcttact cttaaataaa atcattattg tctttatcgt tttgatgact
    16741 ttgcttcctt tactttatat cgtcgtagca tcctttatgg atcctaaggt tctggttagt
    16801 agagggatta gctttaatcc agccgattgg actgtagaag gttaccagcg tgtattcagt
    16861 gaccaatcta ttctaagagg ttttatcaat tctctactat actcttttgg atttgcagct
    16921 ttaacagtat tgctatctgt gtttacagct tatcctcttt ctaagaaaga cttggttgga
    16981 cgtcgttgga ttaactactt cttgattgtg actatgttct ttggtggtgg tttagtccca
    17041 acttacttgc tcgtaaaaga attgggaatg ctcaatactc catgggctat cattgttcca
    17101 ggtgctgtca atgtttggaa tattattctt gctagggcct atttccaagg attgcctgaa
    17161 gaattagttg aagctgctgt cattgatgga gcgaatgact tgcagatttt ctttaaaatc
    17221 atgcttcctc ttgcaaaacc aattatgttt gttctcttcc tctatgcttt tgtaggacag
    17281 tggaactcat actttgatgc aatgatttat atcaaggatc caaacttgga accattgcaa
    17341 cttgtacttc gtaaaattct cattcagagc caaccaggtc aagacatgat tggagcacaa
    17401 gcggctatga atgaaatgaa acgtttagct gaattgatta aatacgcaac tattgtcatt
    17461 tccagcttgc cattgattgt tatgtatcca ttcttccaaa aatactttga taaaggaatt
    17521 atggctggtt cacttaaagg ataaaaaaag aaaaaataaa aggagttttc tcatgaaatt
    17581 caaaacattc tcaaaatcag cagttttgtt gacagctagt ttagcagtac ttgcagcctg
    17641 tggctcaaaa aatacagctt caagtccaga ttataagttg gaaggtgtaa cattcccgct
    17701 tcaagaaaag aaaacattga agtttatgac agccagttca ccgttatctc ctaaagaccc
    17761 aaatgaaaag ttaattttgc aacgtttgga gaaggaaact ggcgttcata ttgactggac
    17821 caactaccaa tccgactttg cagaaaaacg taacttggat atttctagtg gtgatttacc
    17881 agatgctatc cacaacgacg gagcttcaga tgtggacttg atgaactggg ctaaaaaagg
    17941 tgttattatt ccagttgaaa atttgattga taaatacatg ccaaatctta agaaaatttt
    18001 ggatgagaaa ccagagtaca aggccttgat gacagcacct gatgggcaca tttactcatt
    18061 cccatggatt gaagagcttg gagatggtaa agagtctatt cacagcgtca acgatatggc
    18121 ttggattaac aaagattggc ttaagaaact tggtcttgaa atgccaaaaa ctactgatga
    18181 tttgattaaa gtcctagaag ctttcaaaaa cggggatcca aatggaaatg gagaggctga
    18241 tgaaattcca ttttcattta ttagtggtaa cggaaacgaa gattttaaat tcctatttgc
    18301 tgcatttggt ataggggata acgatgatca tttagtagta ggaaatgatg gcaaagttga
    18361 tttcacagca gataacgata actataaaga aggtgtcaaa tttatccgtc aattgcaaga
    18421 aaaaggcttg attgataaag aagctttcga acatgattgg aatagttaca ttgctaaagg
    18481 tcatgatcag aaatttggtg tttactttac atgggataag aataatgtta ctggaagtaa
    18541 cgaaagttat gatgttttac cagtacttgc tggaccaagt ggtcaaaaac acgtagctcg
    18601 tacaaacggt atgggatttg cacgtgacaa gatggttatt accagtgtaa acaaaaacct
    18661 agaattgaca gctaaatgga ttgatgcaca atacgctcca ctccaatctg tgcaaaataa
    18721 ctggggaact tacggagatg acaaacaaca aaacattttt gaattggatc aagcgtcaaa
    18781 tagtctaaaa cacttaccac taaacggaac tgcaccagca gaacttcgtc aaaagactga
    18841 agtaggagga ccactagcta ttctagattc atactatggt aaagtaacaa ccatgcctga
    18901 tgatgccaaa tggcgtttgg atcttatcaa agaatattat gttccttaca tgagcaatga
    18961 caataactat ccaagagtct ttatgacaca ggaagatttg gacaagattg cccatatcga
    19021 agcagatatg aatgactata tctaccgtaa acgtgctgaa tggattgtaa atggcaatat
    19081 tgatactgag tgggatgatt acaagaaaga acttgaaaaa tacggacttt ctgattacct
    19141 cgctattaaa caaaaatact acgatcaata ccaagcaaac aaaaactaga ggttgattat
    19201 gggagataag aaatacacag tagaaaaagc caatcgtttt atagcagaaa ataaacatct
    19261 cgttaatact caatataagc ctgaagaaca tttttcagct gagattggtt ggatcaatga
    19321 tccaaatgga tttgtctatt ttcgtggaga ataccatctc ttttatcaat tctatccata
    19381 tgatagtgtt tgggggccta tgcactgggg acatgctaaa agtaaggact tggtgacttg
    19441 ggagcacttg ccagtggcac ttgctcctga ccaagattat gaccgaaatg gttgtttctc
    19501 aggctctgcc attgtcaagg atgatcgcct ctggctcatg tacactggac atatcgaaga
    19561 agaaaccggt gtccgccaag tgcaaaatat ggcattttca gatgacggga ttcactttga
    19621 aaagattgaa caaaatccag ttgcaactgg atcagacttg ccagatgagt tgattgctgc
    19681 tgattttcgt gatccaaaac tctttgaaaa agatggacgc tattactccg tagtagctgc
    19741 caaacacaag aataatgtgg gctgtatcgt tctactaggg tccgataacc tagtagaatg
    19801 gcagttcgaa tccatctttt taaaaggggt agaacaccaa ggctttatgt gggaatgccc
    19861 agattacttc gagttagatg ggaaagattg ccttattatg tcacccatgc gttatcagcg
    19921 tgagggagac tcatatcata acatcaactc atcgcttttg ttcacgggta aggtagattg
    19981 gggagaaaaa tgttttatcc cagaatcagt tcaagaaatt gatcatggcc aagacttcta
    20041 tgcgcctcaa acattgttgg acgatcaaaa tcgtcgtatc ctgattgctt ggatgcagac
    20101 atgggggcgt acccttccaa cccatgacca agaacacaaa tgggcatgtg ccatgacttt
    20161 acctagaatt ttaagattgg aagatggcaa actaagacaa ttccctgtta aaaaaggcca
    20221 atatcaaatc caaatagata aagattgtca ttaccactta ggaaatgata tagattatct
    20281 tgaatttggt tatgatagta atgcgcagca agtttacatt gatcgtagcc atcttattca
    20341 aaaaattcta ggtgaagaag aacaggacac tagtcgacgg tatgtagata ttgaagctaa
    20401 agaattggaa gttgttctag ataaaaattc catcgagatt tttgtcaatc aaggtgaagc
    20461 aagcttgact gcaacttatt acttaacggt gccagctgag ctatcacgaa ttgattaaaa
    20521 attaagttat ttctcctaaa gaaaaagttc tctttctaaa atagtggaaa gaggactttt
    20581 tgtgttttgg gtatataagc ttagtttatg gtatttgtaa aattggtgtt ggattatgat
    20641 ttaagctagt tttctaaaga atttgaaaaa aattttattt aagcaaaaaa aacccttggt
    20701 tccaaggctt ttcctgttgt atttatatgc cccctacaag tatattatga tacatagtat
    20761 taagtgatta tttatttttg aggcttttct tattctaaaa gtaggtatat gagtttatgg
    20821 acattgtaat tcacaacaaa ttcacggcag aaaatacaat taggtagttt gtaattttaa
    20881 aattaatgcg aaactaatta aatttaaaaa aaatcaactt tcttccttga cctttttaaa
    20941 aaattttggt atacttaatg ggtaatcaag attgaacaaa gaatcaagct ttttcaactt
    21001 gcaaaattat cagaaaggag gctctataat gaggattcaa atgagattat ctaaagaagc
    21061 tcgcgttgtt ttagaagaat taaaattaga atatttaaaa aaaggtgaag acaaaacatc
    21121 aggagtaatc gttgatgaaa tttttaaaca ttttagttct aattttcaaa aagtagattg
    21181 gaaatctgtt caaagtgcac ctaagtttaa tgaaattttg gcagattata cttcagttaa
    21241 tcccacaaca cttaatttat ctaatagtac aataagtaca atgtcttaca ttagagacca
    21301 ttttaactac atattagata tgaaaagaac cgtctatcgt tcattcataa ttcgcctagt
    21361 cttgaaggct tacaaattag agaaggaagg caccgatatt tatttataat agatgtttat
    21421 ttcatctatt ataattttag catgatgagt agtcaaaact atatctaaag ggaggttaat
    21481 ctctctcttt atttttaaaa tttgaaagga aaaatacaaa aatgaaaatt aatgaatatt
    21541 tatggagaac gagctgtctt atactgctaa ttatcatgag cttctttgca ggcttcggag
    21601 ctaaattaga gtttacatct tataacccgt ttttatctgt taaatttggt ttggtcgtag
    21661 ctctgacagt ctttgtagtt caattgttag ccatactgcc agccatggtt aaaagaggaa
    21721 ataaaaatga gaatgttgaa tcaaacaatt cgtgtgaaga gtaaataccc ttacttgatt
    21781 cgtccccgaa atattaaaat tgtaagattg tttatccaaa tttttgtctt tttactctta
    21841 atatcttcgt ggattgctat aatcccattt attgctcgtc tagtcttttc catgtttttt
    21901 ataagtcttc tttttttcgt agagaagttc tttcgttcta attttatagc aaaattgaga
    21961 aacaagctca atatcagaga agccttgttt tacatgttaa ttagtttaaa tttatatgat
    22021 gagctcgata atgaagtagt agatacagca gttttatatt ttgacgttga gaataataaa
    22081 gttgttgtct gtgccccact atttgggaac cgttatctga aaaccttgaa aaatttagag
    22141 gaatatctct gtcccacttt gggcttgtcc ttgctgtcta aaaaagaaga aatcgataaa
    22201 atagtatacg tgttagggca gaaagaagaa atagaacaat atgtttttaa tagtaacaca
    22261 ttaacaagag aattttttaa agatattcct agttcaataa tcaagctgtc aaatacacag
    22321 aaatttagtc taaagtccaa cactaaccta ggtatatatg gaagaacggg gacaggcaaa
    22381 acaatagctc tgcaatggta cttatttaac gcactggcta aaggttgtgg tattgctgat
    22441 aatacttatt taggcatagt agatggtaag gcagctgact tgtatcgtat tggtgaactt
    22501 cttcacgaag aactgggaga acaagtggca gtaggctctt caccacaaat gcttgcccaa
    22561 ttatcgagaa aattcattga gaatatggat gcaagattta aaattatcaa acagaatagt
    22621 tcattgaatg ctgacattta tgagctagat gagctgggac tggtacctaa ttttttgttc
    22681 gttgatgaac tcgcttctat tcgtgatagt tgtggctcta cgaaacaagg tagagaaaca
    22741 tggaatgaaa ttctacaaaa tttaggcttg attgcacgca aaggacggca ggcaggttgc
    22801 cacctagtcc tatctactca agaccctaat gccgaaaaca tccccgttga attgaggaat
    22861 caaatttcag cagttttata ccttggtgcc ccaggggctg atagattgaa gatggctttt
    22921 agcatgtgcg aattagaaaa cgttcctaca gtttcagata gaaaagggga ggctctcttt
    22981 tatgctgatg ggcttaatac agtagagcca gtgctaacaa ttgtgccatt tgttgattta
    23041 aaaactaaga aagagttttt aaatgtagtt agaaacatac tgccaactaa tgacggtctt
    23101 cctcccctag cgaaataggg cgattgccgg aggcagaatc gagcggagcg ctctcttttc
    23161 gctaggggag ggagagccaa ccgactgctt tgcaggaggt cagaaaaaac atccccagta
    23221 ctaatagggg gtgcacacat acatgaaatg tactctaaac ccttgatacg tgaggggtta
    23281 cggcttacta gttgcaccaa ggggtcgcaa actcttggga gagtagggat gagaggtgtt
    23341 tccatgtatg atacggaatt aaaggagaaa aatatggttt acaatatgac agatacaaac
    23401 attaaaattg gattgcttga ccttgagatg tcacagtttg atattcctaa gaaagtagtg
    23461 attttatcag ttagggtgaa caataaatac aaagaagtca acggcgaaaa cgtgaagaca
    23521 gatgaagtaa gtaaaattac ttctgctgtt cttgacgctg acaaagtgaa agttctaact
    23581 gaaatgggaa tttctactga tgacttaaaa gctattaacc tagaaattat cggaaatcta
    23641 gataagattg caaaacttgc tcaaaatgat gctttgttga atgttcctgt tgaattgatt
    23701 cggcctaaag ttcgactagc ttggaacatg gctaggaata attggtctgg tgtaaaaatt
    23761 gtgtgtgagg atattaaagt tctaggagaa taatcctatg aatacagtta gaatagatta
    23821 ttttgctgtg acagttaaga atatttcgcc agaaaaagtt ctgacagaca tccttttgat
    23881 tcctcttgaa aactttacct tgaatgattg gggcataaat aagtataagc aacattattc
    23941 ttgttcagaa attaaggtgt attttaatgc tgataggctt tctatgggtg tatttattga
    24001 attaaaaggg caaggttgtc gtcaatatga agaattttta aacagtaacg aaaataattg
    24061 gattgctttg ataagtcgtt tatatcatca taatgccaac tttactagac tagatattgc
    24121 ccacgacata tttgacggta gcttagatgt tcaacgaata tatgactatt gcaagaaagg
    24181 attatgtatc tcgaaagcaa aacactttga atatcatgag aaatcagttc tagattctgg
    24241 tgagcgtgta ggagaaacag ttgcaatagg ttcacgaggt aatcagcagt ggtgcatata
    24301 taataaacgc atggagcaac ttggtaagca agagcttgta gaagtttcat cttggactcg
    24361 cgcagagctt agatgttggc aggagaaggc taatataatt gcagagcagt tatatttaaa
    24421 acgtccctta tcgtcaatat attttgaggc tatcaacggt cattatcgct ttattcgtcc
    24481 aaatgctata gatagtaatc gttggagaag aaaaaaggtt gagtggtggg taaactatct
    24541 agggacagaa aatcagacag ttctgagtat tacaagggct aagacgacgt tacgggagtc
    24601 tgaagtttgg actgagaaac aggttgcaaa aacattagca aaagtctata ttgcaaagta
    24661 tcaggcctat gatgttcaaa aggcggaaga ttatattcaa aacttgctca aagagggact
    24721 ttcaaagcta actgataatg atgaaaagga tatagaacaa tatataagag aacagaatag
    24781 ttcccaactt tggggaaccg atggaaggag tgactgacat atggcagata ctatatcaaa
    24841 taaggatttg atttccatgg gctataggcc atcaacagct aatgctatta tccatcaggc
    24901 gagagaatta cttgtatcac gaggctatac attttataat cgcaaacgtt tgatggttgt
    24961 tccaaaaagt gttgtgaaag agttgttggg aatggaattg tgaaatggct tatatcgagt
    25021 ataaacagcg tggtaagaaa agactttggt ccttctctat acgagaaagg agcaagagcc
    25081 tactccataa aagcggattt aaaacaaaac gagaagctaa aatagaagcg gagaaagttc
    25141 ttcataagtt aaatactggg agtgtcttaa gctctagtat gactttatcg gagctttata
    25201 atgaatggct tgatttaaaa attttaccta gcaatagaag tgtagttact aaaaaaaaat
    25261 atctcatgag aaagaaggtc atcgaaaggt tatttggaaa taagcctgta tcacaaatta
    25321 agcctagcga gtatcaaaaa attatgaatg agtatggaga gactgtatcg agaaatttct
    25381 taggaagatt gaattcttgt attcgggcaa gtatacagat ggctattgct gataaggtga
    25441 taatagagga ttttactgct tatgttgagt tgttctcttc taagagtgga caaaaggttg
    25501 aggaaaagta tttgcatgct gagtcagatt atcaaaaagt tttagtatat ttgaaaaata
    25561 agtttgatta tcaaaaatct attgtaccgt atgtaattta tttccttttt aaaactggca
    25621 tgcgtttctc cgaattgatt gctctaactt gggatgaagt tgacgagctt aatgagcaac
    25681 ttaaaactta tcgtcgttac aacaccgcta ttcataagtt tactccacct aaaaacaata
    25741 cttcaataag attagttcct attacttcag atatgctttc cttgttaaaa acattgaaaa
    25801 tattgcaatt aaaaacaaat aaagaattaa atattgataa tgaaaataat ttgatttttc
    25861 agcattttgg ctatgtctac gatgttccag atattgcaac agtcaataaa gctataaaag
    25921 taatgttgaa ggaattgcaa atttttccat tgattactac caaaggagct agacacactt
    25981 atggtagtta tttatggcat aataatattg atttgggagt tatagcgaaa attttaggac
    26041 ataaagatat ctccatgttg attgacgttt atgggcatac cttggaggaa aaaattagtg
    26101 aagagtttat ggctgtgaaa agtttgttgt aaaaacacgg caaattgcac gacaattttt
    26161 aaaaggaagg caaaaaaagc cctatattat aaggcttttc ctgttgtatt tatatgcccc
    26221 ctacagggat cgaacctgtg acccacggat taagagtccg ctgctctgcc agctgagcta
    26281 aggaggcaaa gaaaaaagct gtattggtgc cgaaacttca cggtttgtat tgaacccgcg
    26341 caattaagca ggtgggcaac tcgctctaac tgaagctgtt tccgtgtgag acggcctaca
    26401 tgctgttaga agacttttgt ttccctaata atacaaaaaa tagtcggtca acacttaagt
    26461 gtgaagtcgt acaccacagc gtttctatac ttatatgata ccactttttc aaaaaaaatc
    26521 aagaggaaag tgcaattttt tgaaaaggat ttcagatttt tcgcaaacgg tcgatagctt
    26581 cctttctttg agccaaagcc cgttcatatt taccagtatc ttctgcttgg aaatagtgat
    26641 gattacgaat tttttctggc agatagtctt gcttgaccca atttccagga tagttgtgtg
    26701 gatagagata gtcttgggca ttccccagtt ccttgcttcc actgtagtgc ccatcacgta
    26761 ggtgtcgcgg aataggcaag tgccctgatg ttttgaggtc agcaagtgcc ttatccatag
    26821 ctacataggc tgagttggat tttggagaaa gggccaaatc aatcacgaca ttggcaatga
    26881 gaatgcgggc ttctgggaac ccaatcttct gggcagcatc cagagcagtc acggtatgaa
    26941 tctgggcctc aggattggct aagccgatat cttcataggc gataacagtc aagcgacgag
    27001 cgagactagg cagatcccca gcctcaatca agcgggcagt atagtggaga ctggcatcca
    27061 catctgagcc acgaatagac ttttgcaggg cagatagaac atcatagtga ccgtctccat
    27121 ccttgtccat agtgatgtag cttctctgaa gactattttc catgatgtct aaggtgatgt
    27181 ggcgaatgcc ctcgtcattc tcaggggtag agagaacagc taagtccagt gagttaaagg
    27241 cagagcgaag gtctccgttt gtagaggttg cgatgaaatc cagcgcatcc tcatctagtt
    27301 ctattggaaa atcaaaacca cgttcagggt tacttagagc tatctgcagg gcctctttga
    27361 cgtcttggtt agacagaggt tccaactcga aaatttgaac tcggctacga atggcaggag
    27421 tgacagagaa gaaaggattt tcagtcgttg ctccaatcat gatgaccagt ccactttcca
    27481 agagaggcaa gaggaagtct tgcttggtct tatctagtcg atgaatttcg tctagcaata
    27541 ggacgagacc accagaaaat ttagcttctt ccgagatttc ttgcagtcgc tttttactat
    27601 caactgtcgc attgaaagtt cgaaaggcat acttggtcgt tccagcgatg gcagaggcaa
    27661 tactggtttt gccgattcca ggagggccat atagaatcat ggaggacagg cggttggctt
    27721 ccaccatgcg gcggatgatt tttccaggtc cgaccagatg ctcctgaccg atgacctggt
    27781 cgatggtttt agggcgcatg cgaagcgcga gattgtctgg catagcagtc ctttctagcg
    27841 tggatttgag tagaatgact catctactat atcttactat gtccattcta gcctattagt
    27901 tgtgatattt caatggtttt gtaataatca gggagtgaga tagaatcttt tttactattt
    27961 aatttaatga ggagaggtat cagcatgaga gatttgattt tccttaaagc tcaaaatagg
    28021 atagttgagc ctaaagaagt ggaagtgctg acttgagaaa taatattgct gaatgtcatt
    28081 gtatgaacta taatgaaaag agaggtgata agcaatggaa atgatggacc aagtcaggaa
    28141 attttgattt ttacaaaaca aattcgtcat tggattctta gtgatcaagt tatttcaggg
    28201 aaaagaaaac ttttcttccg ggaagatact ccaaaagaga ttttagattt gtacgaaaac
    28261 attaaatcta aacttgattt tgcttaccaa taagtttatt ctaataatgg gttgaaaaaa
    28321 tatgaaaaat agaataattg atgtttttga agtagtaaat cgcatccttg taataactgt
    28381 tgaaaatcct gatttcgaag acttgagagt taatcatact caatgaaaat caaagagcaa
    28441 actaggaaac tagccgcagg ctgtacttga gtacggtaag gcgacgttga cgtggtttga
    28501 atttgatttt cgaagagtat cagtttgtaa aaattggaga taaaaaatat agagtacgca
    28561 gtgttcctat gattcattca actccgcctc aatctgtctt agatcgagat acttttacaa
    28621 ttgactatac agatgatgaa ttgctcgata aagaagcagt ttttactact cattagaaaa
    28681 ataggtctga ataaattggc ttcccttaga accacatcta ggggctttta tgtataatag
    28741 aataataagt atcactcctt tacaatacgt gtaacacgtg ttataatggt gttgtcagga
    28801 ggtaaaagcg ctatgcctat gacacaaaaa gagatggtta aactccttac ggcccatggt
    28861 tggataaaga ctagaggcgg taaaggctcc catattaaaa tggagaagca aggggaaaga
    28921 cttatcacaa tccctcatgg tgagctgaat aagtacactg aaagagggat aagaaagcaa
    28981 gctgggttgt aaaattcagc ccctgttttt tgatatggag gtagtgaaat gttagttacg
    29041 tatccagctt tattttatta tgacgacaca gacggaacag aagcgactta ttttgtccat
    29101 ttcccagatt ttgaatactc agctacacaa ggagagggga tttctgaggc tttggctatg
    29161 gggtcggagt ggctagggat aactgttgca gatttgatag agagtgatgg agagttacct
    29221 cagccatcag atatcaatag cttgtcttta attgataatg atccttttaa agatgatgaa
    29281 gatttcgtgt caacctatga ccttgataaa tcttttattt ctatggtatc tgttgatgta
    29341 tcagaatacc taggaagtca ggaacccata aaaaagactt tgaccatacc aaaatgggca
    29401 gataagttgg gacgagaaat gggacttaac ttttctcaga ctttgacaga cgctattgca
    29461 gataagaaag ttcaagccta agattaagcg tggtatcgtg gtaatagtca ataaaaaagc
    29521 acgtccactt gtgctagtta ctcgcctatt gattttatag atttattagc cctttcgcgg
    29581 gctttttata ttgattttta tgagaaatga agaaatcaga cttttaagaa aatcactgat
    29641 accaagggtt taaatgaggt aatatggtat aattaggaca taaaataatt ttgtggtaag
    29701 atggtagtat ctattttagc atatttccga gcaatcgggg cgattaaaga gtcgcataga
    29761 aagaggacaa aatggcaaca tacggatttt tagatgtttt agaggaagag ttggacaaga
    29821 actttccctt tgactttgag attagctggg acaaacgcaa ccatgcggta gaagtgagtt
    29881 ttctattgga agcgcaaaat gctgcaggtg tggagatggt ggatgaagac ggagaggttt
    29941 tatcagatga cattcttttt gaggaagcgg tccttttcta caatcctgcc aagtcaacag
    30001 tcaatgagga agactatttg actgttattc cttacctgcc taaaaaagga ttttcccgtg
    30061 aatttttagc ttattttgcg cttttcctta aagatactgc tgaggttggg ctagatgtcc
    30121 tcatggactt tttggaagac ccagaagcag aggaatttgt catggaatgg aaccaagaag
    30181 tctttgaaga aggaaaagtt ggcttggaag agggagaatt ttatccttat ccaagatact
    30241 aggagttggt tggaggtgtt atgaagaaaa ttgggactta tttggtttat gtgctagctt
    30301 ttgtctttat tatgctagct tttgcttgtg gaacaatcgc atttgcagag ttggggtatt
    30361 ccgcagtttt agcctttact tttggttatg ccttcgctct tctaagcatg tatttaatct
    30421 ttattcttca tgagctgggt catgcttttt gtggctactt gacaggctat cggctggtgg
    30481 cttttggatt aggacatttt attttgacca aaaagtcagg caggtttcat cttagtagaa
    30541 cagccattct gaaaaatgtt ggtgctcaat acattggttt aaaagaggat gaaagcgatc
    30601 aaagaatcat cctgatgctt tcaggaggct tgatggttca tctcggcttg atattattgg
    30661 cgatagtgtt tggatttttg acaagaagct ggtattttgc agggacttgg atttttctta
    30721 atttatcttt cttcctaaat aacattttgc cagtcgacat caccgatgga gcaaaaattt
    30781 gggaattact acaacaccct gaaaatacca aatacgccta cctggtattg agacattctg
    30841 ctcaaacttt gctggctcct caagaatatg atttgaaaga ctttgttcaa gcagttcctg
    30901 aggatgcacg agggagtttt gcggatggtg tattaggtat gcaaggtgaa gtttccattt
    30961 tggaagggaa ggaagaagta gctaaacagc agtttcaagc attacttgag aggacaacta
    31021 ctccgatgat gcagacagtt gcccaactat ccctgcttca tatcgccttg ttggaaggag
    31081 attttgagca agcggagcaa tatgctagta ttcgacaggt caaatcgttt ctgtctctca
    31141 aactgtccaa tcttcaagtg atacaagctt ggtatcaatt taaggtaaag aaagatgtag
    31201 atcgaactcg taaggctctc aagattgcta gacagaaaat ggatagtagt cgaatgttga
    31261 gggacgagaa acgctattat gaaaacttgt tagcagagct ggaaaaggaa atgttagaag
    31321 gaactgaaga tggaactaga aatttctgat ttcccaggct gtaagattgc tttgttttgt
    31381 ggggataagc tcttgactat cttacgtgat gataaggcaa gtattccctg ggccaatatg
    31441 tgggaactgc caggtggtgg acgtgaaggt gatgaaagtc cttttgagtg tgtagcgcgt
    31501 gaagtttatg aagaactggg cattcatctg actgaagatt gtctgctttg gagtaaggtt
    31561 tacccaagta tgctttttgc agacaaacag tccgtttttc tagttggtca gttgacacag
    31621 aaccagtttg acagtatcgt atttggagat gaaggacagg gctatcagct gatgaatgtt
    31681 gaggaatttc ttagttccag tcaagttgtc cctcagttgc aagagagatt aaaagattat
    31741 ttaaaagtaa gtgattagaa aggatggttc agttaaattt ctaaactgaa cccgccctaa
    31801 acactgtgcc aaaaagataa acttctctta gacacaagcg tcttcagaga atttcctatt
    31861 ttggttttgt gttttacggg cttggtatct taataatgga aacatggcaa gagttaaaag
    31921 ttacagtgaa gcgtgaggga gaggagttgg tttccaatct cttgattgag ctgggagcgc
    31981 aaggagtcgc aatcgaagac agtatggact atgtgggaaa tgttgaccgc tttggcgaga
    32041 ttttcccaga ggtggagcag caagaagaaa tcgttgtgac agcctactac cctgacacgg
    32101 tggatgtgac agtggttgag gcggacttgc aggctcgcat atcagaattg acggatttta
    32161 tggatttggg agagttaaaa ataggtacga ctgccttggc tgaggaagac tgggcagaca
    32221 actggaagaa atactatgag ccagctcgta tcactcatga tttgaccatc gttccctctt
    32281 ggacagacta tgaggcgact gcgggagaaa aaattatcaa gctggatcct ggcatggctt
    32341 ttggtactgg aacccaccca actaccaaga tgagcctttt tgccttggaa caggttcttc
    32401 gtggtggcga aacggtgcta gatgtgggga ctggttcagg ggttctctct attgccagct
    32461 cgcttctggg ggctaaagaa attttcgcct atgacctgga tgatgtggcg gttcgtgtgg
    32521 ctcaggaaaa tattgagctc aaccctggca tggaaaatat ccatgtagcg gctggagatt
    32581 tgcttaaggg tgtggaaatt gaggcagatg tgattgtggc taatatcttg gcggatatcc
    32641 tcattcatct aatagacgat gcctatcgtt tggttaagga cgaaggctac ctgatcatga
    32701 gtggcattat caaggacaag tgggacatgg tgcgccagtc ggctgagtca gctggatttt
    32761 tcctcgaaac tcacatggtt caaggggaat ggaatacctg tgtctttaag aaaaccaagg
    32821 atatctctgg tgtgattgga ggctagcatg cagcagtatt ttgtaaaagg cagtgctatc
    32881 tctcctgtca ctatcgagga caaggaaacc agtaagcata tgtttcaggt tatgcgcttg
    32941 aaagaagagg atgaggttac cttagtcttt gatgatggca tcaagcgctt ggcgcgcgtg
    33001 ctggatatgg aaaatcgtca gtttgagttg gtccaagaat taaatgacaa tgtggaacta
    33061 ccagtccaag tgaccatcgc atctggattt cccaagggag acaagctgga gttcatcgct
    33121 caaaaagtaa ccgaactggg tgctagccaa atctgggcct ttcctgcaga ctggtcagtt
    33181 gccaagtggg atggcaagaa attgggtaaa aaagttgaaa aactagaaaa aattgccctt
    33241 ggagcagccg agcaaagcaa gcgtaatatt gtaccaagta ttaaactttt cgagaaaaaa
    33301 gcagattttc tagctcagct ggaccagttt gactttatca tagtagccta tgaagaatca
    33361 gctaaagaag gagaagccgc tgcgcttctg caagcagtca ctggtcttga aacaggagcc
    33421 aaactgctct ttatctttgg tccagaaggc ggtctctcac ctgcagaaat cgaaagtttt
    33481 gaagctaagg gagccgtttt ggtaggccta ggtcctcgta ttttgcgagc agaaacagca
    33541 ccgctttacg ccttatcagc ccttagtgtt ttagtagaat tagagaaata agaggaagaa
    33601 aatggaacaa aaacaccgtt cagaatttcc agagaaggaa ctctgggact taacagccct
    33661 ataccaagac cgtgaggatt tcttgcgtgc aatcgagaaa gctcgtgaag acatcaacca
    33721 gtttagccgt gattacaagg gcaatcttca cacttttgag gatttcgaga aggcctttgc
    33781 ggaattagaa cagatctaca ttcagatgag ccatattggc aactatgctt ttatgcctca
    33841 gacgacggac tatagcaatg acgaatttgc caatattgcc caagctggga tggaatttga
    33901 aacagatgcc agcgtagcct tgaccttctt tgacgatgcc ttggtggtag cagacgagga
    33961 agtcttggac cgtttgggtg aattgcctca cttgacggca gctattcgtc aggccaaaat
    34021 caaaaaagcc cactatctag gggctgatgt ggagaaggcc ttgaccaatc tcggtgaagt
    34081 tttctatagt ccgcaggaca tttatactaa gatgcgagct ggggattttg aaatggctga
    34141 ctttgaagcc catggcaaga cctataaaaa cagctttgtc acctatgaga atttctacca
    34201 aaatcacgag gatgctgagg ttcgtgagaa atccttccgt tccttctcag agggacttcg
    34261 taagcaccaa aatacggctg cagcagccta tctggctcag gtcaagtctg aaaaactctt
    34321 ggctgatatg aagggatacg actctgtatt tgattatctt ctagctgaac aagaagtgga
    34381 ccgtgtcatg tttgaccgcc agattgacct catcatgaag gactttgcac cagtcgctca
    34441 gagatacctc aagcatgttg ccaaggtaaa tggtcttgaa aagatgacct ttgcagactg
    34501 gaaattggac ttggacagcg ccctgaatcc tgaagtgact attgacgatg cctatgattt
    34561 ggtcatgaag tcggtagaac ctttggggca agaatattgt caggaagttg ctcgttacca
    34621 agaagagcgc tgggtggact ttgctgctaa cagtggcaag gattccggtg gttatgcggc
    34681 ggacccatat cgcgtacacc cttatgtact catgagctgg acaggccgtt tgagcgatgt
    34741 ctataccttg attcatgaaa tcgggcattc tggtcaattc atcttttcag acaatcacca
    34801 aagctatttc aacgcccaca tgtcgaccta ttatgttgaa gcaccgtcaa ccttcaatga
    34861 attgctactc agtgattact tagagaacca gtctaatgac ccacgtcaaa aacgcttcgc
    34921 tctggctcat cgcttgacag acacctactt ccataacttt atcacccacc tcttggaagc
    34981 cgccttccag cgtaaggtgt atacattgat tgaagaaggg gagacctttg gagcaagcaa
    35041 actcaacagc attatgaagg aagttttgac agatttttgg ggcgatgcca ttgaaatcga
    35101 cgacgatgca gctttgactt ggatgcgcca agctcactac tatatgggct tgtatagtta
    35161 cacttactct gctggtcttg tcatctcgac agctggttac cttcatctta aaaactcaga
    35221 aactggagct gaagactggc tcaatctcct caaatcaggt ggtagcaaga caccacttga
    35281 gtcagccatg attatcggtg ctgatatttc aacagacaaa ccactccgtg ataccatcca
    35341 attcttgtct gacacagttg accagattat cgcctacagt gctgagttgg gggagtaagg
    35401 ggatagagag gttgttatag aatgggtatg gacagttatt tgcttaaaag tccatgttta
    35461 aatcccccat tccttttcga ggaattcttt gccctgctct gtaatggtaa gtgaacgtgg
    35521 cgtttgtgca gggatgacca attgtttttc actgaaaaat tctaaaagag cattgccgag
    35581 agctccgccg acatgatttt tacgttcgct ccaatccaaa catttagggc aaaatttccg
    35641 ttttttcttt ttcaattcat ccgtatcaat gtgtaatttt ttttggaaat aagacttccc
    35701 agcttcagtg agttggaagt ctttttctga ttcgctgagg agattttctt gcaagagata
    35761 attggtaatg gaaatgccaa gctcaccagc aatatggtca tagcaggtgc gagcgacctt
    35821 taattccttt ttgtgaatgt gttgtttata acttttgaca tcacgctgag gagagtagaa
    35881 gctgagaaat tccagcattt ctgcaatggc agcacttttt aaagaataat aatgaaaacg
    35941 tccataggtt tgtagagtgg ttacctgcgc ttcggtaagt aatttgaggt gatagctgac
    36001 agtatgttgc ttgattttgg taaagctggc aatttcgtta accgtgtgag cttttccatc
    36061 cattagaata tctaaaatgt gcatccgaga agggtctgat aagagttttg tgatgatgaa
    36121 aatatctggt tccataaaaa ttttctccaa atttcatata tcgattattt tctatctttt
    36181 attatactat aatcaaagca caagaaaaat agattttaaa aatgttttac tagagaatat
    36241 tttaaatttt attgtctgag tttaaatgga ggtaaaatta caaaagtaat tgcagcagta
    36301 tccacagttg aaggacgtta atcttaagat tacattctca acattttttc aaaagtgaga
    36361 tgaattaaca aagtatccaa catgctctat ttgttgggga aagaaagagt gaagaagtta
    36421 agtgttaaaa atctaaaaaa gcaatatggt tcttatgaag ctgtcaaggg aattagcttt
    36481 gaattgggag aaggagagtg tttgggtatc ttgggaatca acggtgctgg caaaacatcc
    36541 actttaaaga tgatttactc actgacctca atttcctcag gagagattga tatcttgggg
    36601 caaaatattg aaaccaatcg tcgtaagagt aaatcttgtc tgggtattgt agggcaggag
    36661 gatatgctgg atatgacctt gaatgtttat gataatcttt ttgctcacgg tctatgttat
    36721 ggtatctcat ctgtcgaact taggaagaga atcgattttc tcttggattt tgttcaactg
    36781 agcaaacatg cgcaaaagat ggtgaatcag ctgtcgggtg ggatgcgacg acgtcttgtc
    36841 ttggcaagag ctctcatcaa tcggcctgat attatcattt tagatgaacc gacagttggt
    36901 ttagacattc aatcccgcaa tattatttgg gataaacttt tggagttgaa ggaattaggt
    36961 gtttccatca ttattacttc ccactatatg aatgagattg aatatttgac agatagggtg
    37021 ctgatgctcc accaagggac tatcaaagaa gaaggaactg tagaagactt gctcatcaag
    37081 tatgatgctg acaaccttga gacgcttttt ttggggttga ctgggacaga gaaggaggac
    37141 ttgcatgttt aagttatcat ttcactccat cggacacgtt gtggtacgca actacatgag
    37201 ttttagaaat ttatttaaaa tttcaattgt ccccaacctt atcgaccccc tcttttatct
    37261 tttagcaatg ggatttggag ttggtgctta tttgacgcat gttaacggta tgctctaccg
    37321 tgattttgtc attacaggtt taattgctgc gactgccatg tctgcggcca ctgcagaaac
    37381 aacggtcaat gcctttatcc agtataagat tgaaaagact tatgatgcta tgttgatgac
    37441 gccaatcaat acgaatgata ttgtggtggg tcaggctatc tgggcaggta ttcgtgcagt
    37501 gattttcggt gggatttttt ggctgatttc catgcttatc acagcgcatt ttcatgtctt
    37561 gatgttattg attcctctga ttttatttat agtgggttat ttgtttggtg ttttgggact
    37621 aatctttacc tacttggcgc cgtctaggga atttctgaat tactacaatg ttttggtaat
    37681 ccgtcctctt tatatgtttt cagatacctt ctttcccatt acttccttgc ccccaatact
    37741 tggtgatttg acttggtttt ctcccttgta tcacgctacc cgcatgattc gtattatatg
    37801 gggaggtgag gtgacaggtt tgtatcttca tcttatctgg ttgatagcct tggcgctctt
    37861 gataacggtg cttcctatgt atatgttaca tcgtagatat tatagatagt ctaattattt
    37921 gtgatgttgt agtcatattc taatttatac taaacgttat tcgaatttgg acaggtatgg
    37981 gaactgtgcc tatcaatatt gtaatttatg gaagttgaaa tagtacggtt cagtttttaa
    38041 aatattgtta gaaattgttt taaattccct aatcaatttg tatagttttt atttcacttt
    38101 attataacta gtttttcgca atttttgaga caggaatgtg atttttgtga taaggctcta
    38161 tcattacaag ttcatttgtg gtcatatgga ataaatcatt tatatatctt ttcagaaata
    38221 tgctgtttct ctgaatataa atgtcttttt ctttttaact taattttaca aattggtaaa
    38281 gtcatttaag actgcctttt tgaacagttg ctagaaatgt agaggtattg accatgtatc
    38341 aagatttact aagaaaaata gcagaagaaa aaccaaatta taaccaggaa gaaatccagt
    38401 ggttgtttga tcatttggga aatccttctc cagaaattcg tgatgacctt gtttttacaa
    38461 gctttgctaa ggagattcag gaagagctat ttacacagga acaatttcat ttcattgctg
    38521 aggtggtctt agctgatgga gggttagata aagagattga taaggtaggc ttgtcaacac
    38581 ttgaacgttc ttttagggca cttatttatg caaatctctt gtctgcagat gctaaccagc
    38641 aatcggtttt ttatcaggga ttacaatcag aaattcgtaa tgtcctttta aatcaaggct
    38701 tgcactatct ttcaaaagaa aaggatacaa caggtttctc aagtcagtat ggttgggttc
    38761 attcttttgc acatggagcc gatttactga cagaggtggt ctgtcatcca gactttccta
    38821 taaacagaat tcatgaagta tttgatatac ttggccaact atttaaaaga atgtcgattc
    38881 tctttacaga tgatgaggat tggcgtttag caagagtgat ctatgaacct attttacaag
    38941 ggaagttgga gcaagaacaa gtagcttctt ggataaaaac tgttgatttt ccgatagaag
    39001 aaagggagga tttttacaaa ttttccaact ttagatcctg tctggtggaa gtctatgtcc
    39061 aacttgacca gagaaatagt ttacaagatg acctgaaaga agctatccag tcttttcaat
    39121 actagggatt agctaatagt tctatgattt tcaaaaaatt tccaatcaat tttcttgaaa
    39181 ccctttccta cttttgatag actagtatct agtttttgaa aaaaggagaa agaaaatgaa
    39241 aaaatttgtt gctgagttaa tcggtacgtt catgcttgtg ttcgttggga caggagctgt
    39301 tgtttttgga aatggtcttg atggccttgg tcaccttgga atcgcctttg cctttggttt
    39361 ggcaatcgtg gtggcagcct actcaatcgg aactgtttca ggtgctcact tgaacccggc
    39421 tgtttcgatt gctatgtttg caaacaaacg tttgtcatct tcagaacttg taaactacat
    39481 ccttggtcag gttgttggag ctttcatcgc ttctggcgca gtcttcttcc tcttgggtaa
    39541 ttcaggtatg tcaactgcta gtcttggtga aaatgccttg gcaaacggtg tcactgtctt
    39601 tggtggtttc ttgtttgaag tcatcgcaac tttcttgttt gtcttgatta tcatgactgt
    39661 gacttcagaa agtaagggca atggcgcgat tgctggtttg gtaatcggtt tgtcattgat
    39721 ggcgatgatt cttgtcggac tgaagattac tggactttca gtaaacccag ctcgtagctt
    39781 ggcaccagct gtcttggtag gcggcgcagc ccttcaacaa gtttggattt tcatccttgc
    39841 accaatcgct ggtggagttc ttgcagccct tgttgcaaaa aatttccttg gaacagaaga
    39901 ataattgaaa ctcaaaaagc cttgctcctc atcttgagga acagggcttt ttcgtatgat
    39961 actcttcgaa aatctcttca aaccacgtca gcttcacctt gccgtaggta tggttactga
    40021 cttcgtcagt ttcatct
//
LOCUS       DBEJAQ010000014        39754 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.014, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000014 DBEJAQ010000000
VERSION     DBEJAQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39754
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.014"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            9..89
                     /locus_tag="ACL297_000795"
     tRNA            9..89
                     /locus_tag="ACL297_000795"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="ACL297_000796"
     tRNA            94..164
                     /locus_tag="ACL297_000796"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="ACL297_000797"
     tRNA            179..251
                     /locus_tag="ACL297_000797"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            265..336
                     /locus_tag="ACL297_000798"
     tRNA            265..336
                     /locus_tag="ACL297_000798"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Gln,seq:ttg)
     gene            346..429
                     /locus_tag="ACL297_000799"
     tRNA            346..429
                     /locus_tag="ACL297_000799"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:380..382,aa:Leu,seq:caa)
     gene            536..1813
                     /locus_tag="ACL297_000800"
     CDS             536..1813
                     /locus_tag="ACL297_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HGR0071085.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1889..3373
                     /gene="thrC"
                     /locus_tag="ACL297_000801"
     CDS             1889..3373
                     /gene="thrC"
                     /locus_tag="ACL297_000801"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGR0071086.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3434..4714
                     /locus_tag="ACL297_000802"
     CDS             3434..4714
                     /locus_tag="ACL297_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGR0071087.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4718..5338
                     /locus_tag="ACL297_000803"
     CDS             4718..5338
                     /locus_tag="ACL297_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGR0071088.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5504..6616
                     /locus_tag="ACL297_000804"
     CDS             5504..6616
                     /locus_tag="ACL297_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGR0071089.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6939..7364
                     /locus_tag="ACL297_000805"
     CDS             6939..7364
                     /locus_tag="ACL297_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGR0071090.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7375..7734
                     /locus_tag="ACL297_000806"
     CDS             7375..7734
                     /locus_tag="ACL297_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HGR0071091.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            7841..8485
                     /gene="pcp"
                     /locus_tag="ACL297_000807"
     CDS             7841..8485
                     /gene="pcp"
                     /locus_tag="ACL297_000807"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGR0071092.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(8513..9370)
                     /locus_tag="ACL297_000808"
     CDS             complement(8513..9370)
                     /locus_tag="ACL297_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGR0071093.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9500..10642
                     /gene="tgt"
                     /locus_tag="ACL297_000809"
     CDS             9500..10642
                     /gene="tgt"
                     /locus_tag="ACL297_000809"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGR0071094.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10740..12557
                     /locus_tag="ACL297_000810"
     CDS             10740..12557
                     /locus_tag="ACL297_000810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGR0071095.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12710..13861
                     /gene="nagA"
                     /locus_tag="ACL297_000811"
     CDS             12710..13861
                     /gene="nagA"
                     /locus_tag="ACL297_000811"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGR0071096.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14024..15082
                     /locus_tag="ACL297_000812"
     CDS             14024..15082
                     /locus_tag="ACL297_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HGR0071097.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15233..15598
                     /locus_tag="ACL297_000813"
     CDS             15233..15598
                     /locus_tag="ACL297_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HGR0071098.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15674..16615
                     /gene="comGA"
                     /locus_tag="ACL297_000814"
     CDS             15674..16615
                     /gene="comGA"
                     /locus_tag="ACL297_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HGR0071099.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16563..17579
                     /gene="comGB"
                     /locus_tag="ACL297_000815"
     CDS             16563..17579
                     /gene="comGB"
                     /locus_tag="ACL297_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HGR0071100.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17581..17907
                     /gene="comGC"
                     /locus_tag="ACL297_000816"
     CDS             17581..17907
                     /gene="comGC"
                     /locus_tag="ACL297_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HGR0071101.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            17900..18304
                     /gene="comGD"
                     /locus_tag="ACL297_000817"
     CDS             17900..18304
                     /gene="comGD"
                     /locus_tag="ACL297_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HGR0071102.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18267..18569
                     /gene="comGE"
                     /locus_tag="ACL297_000818"
     CDS             18267..18569
                     /gene="comGE"
                     /locus_tag="ACL297_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HGR0071103.1"
                     /translation="MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQE
                     EAKILQKEEVLRVAKMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18532..18993
                     /gene="comGF"
                     /locus_tag="ACL297_000819"
     CDS             18532..18993
                     /gene="comGF"
                     /locus_tag="ACL297_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HGR0071104.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            18971..19417
                     /gene="comGG"
                     /locus_tag="ACL297_000820"
     CDS             18971..19417
                     /gene="comGG"
                     /locus_tag="ACL297_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HGR0071105.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            19520..20107
                     /locus_tag="ACL297_000821"
     CDS             19520..20107
                     /locus_tag="ACL297_000821"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGR0071106.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            20168..21121
                     /locus_tag="ACL297_000822"
     CDS             20168..21121
                     /locus_tag="ACL297_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGR0071107.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21172..22362
                     /locus_tag="ACL297_000823"
     CDS             21172..22362
                     /locus_tag="ACL297_000823"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGR0071108.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYFAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22363..22494
                     /locus_tag="ACL297_000824"
     CDS             22363..22494
                     /locus_tag="ACL297_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071109.1"
                     /translation="MKLKKYSIQGVGKVIFPASFSDEIVQNLAMIGFFEKYGIIVVI"
     gene            22511..22882
                     /gene="rnpA"
                     /locus_tag="ACL297_000825"
     CDS             22511..22882
                     /gene="rnpA"
                     /locus_tag="ACL297_000825"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGR0071110.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22857..23681
                     /locus_tag="ACL297_000826"
     CDS             22857..23681
                     /locus_tag="ACL297_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGR0071111.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23700..24686
                     /gene="jag"
                     /locus_tag="ACL297_000827"
     CDS             23700..24686
                     /gene="jag"
                     /locus_tag="ACL297_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGR0071112.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24737..25360
                     /locus_tag="ACL297_000828"
     CDS             24737..25360
                     /locus_tag="ACL297_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HGR0071113.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25491..26948
                     /locus_tag="ACL297_000829"
     CDS             25491..26948
                     /locus_tag="ACL297_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGR0071114.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            26971..27252
                     /locus_tag="ACL297_000830"
     CDS             26971..27252
                     /locus_tag="ACL297_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0071115.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27329..27814
                     /locus_tag="ACL297_000831"
     CDS             27329..27814
                     /locus_tag="ACL297_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGR0071116.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27831..28496
                     /locus_tag="ACL297_000832"
     CDS             27831..28496
                     /locus_tag="ACL297_000832"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGR0071117.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28500..29363
                     /locus_tag="ACL297_000833"
     CDS             28500..29363
                     /locus_tag="ACL297_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGR0071118.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29365..30069
                     /locus_tag="ACL297_000834"
     CDS             29365..30069
                     /locus_tag="ACL297_000834"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGR0071119.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30248..31921
                     /locus_tag="ACL297_000835"
     CDS             30248..31921
                     /locus_tag="ACL297_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGR0071120.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINKEIVVQAFLEDI"
     gene            32033..33124
                     /gene="ulaG"
                     /locus_tag="ACL297_000836"
     CDS             32033..33124
                     /gene="ulaG"
                     /locus_tag="ACL297_000836"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGR0071121.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33238..35214
                     /gene="tkt"
                     /locus_tag="ACL297_000837"
     CDS             33238..35214
                     /gene="tkt"
                     /locus_tag="ACL297_000837"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGR0071122.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35331..35630
                     /gene="yajC"
                     /locus_tag="ACL297_000838"
     CDS             35331..35630
                     /gene="yajC"
                     /locus_tag="ACL297_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGR0071123.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLQLKETASLEGAIEK"
     gene            35677..36105
                     /locus_tag="ACL297_000839"
     CDS             35677..36105
                     /locus_tag="ACL297_000839"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HGR0071124.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLCQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36107..36517
                     /locus_tag="ACL297_000840"
     CDS             36107..36517
                     /locus_tag="ACL297_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGR0071125.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36817..39468
                     /gene="adhE"
                     /locus_tag="ACL297_000841"
                     /gene_synonym="adhC"
     CDS             36817..39468
                     /gene="adhE"
                     /locus_tag="ACL297_000841"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGR0071126.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12111 a         7099 c         8922 g        11622 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttattggggt atagccaagc ggtaaggcaa gggactttga
      301 ctccctcatg cgttggttcg aatccagcta ccccagttac tatttgccgg cgtggcggaa
      361 ttggcagacg cgctggactc aaaatccagt gtccgcaagg acgtgccggt tcgaccccgg
      421 ccgccggtat agtatagtgt taggaacgtt gttattcttc gttccttttt tatattattt
      481 ttggtataat tatagttatt caaattttat ttagattaag aaagtgtagg ggagtatgtc
      541 ttgttctatc gatttattaa aacatcggta tttgaaaaat attaaagaaa atcctgaatt
      601 gtttgtcgga attgagttgg agtatcctgt tgcaagttta gaaggggatg ctacagatgt
      661 tgaagttatg aaggatctat ttcattattt agtttctact ttggatctca ccgtagcaaa
      721 ggtagatgat tttggcaatc tgatccagtt agtagatccg ataagtcagg atgctatttt
      781 atttgaagtt tcctatacaa cgattgagtt tgcatttggt aaggctgaaa cgattcaaga
      841 ggtcgaaaat cgtttcaata attatatgaa tgtaattcag agaaagttag ctgaatcaaa
      901 tcatgctatt gttggctgtg gtatccatcc caactgggat aaaaatgaga attgtccagt
      961 ggcttatcca cgctatcaga tgttgatgga ttatttgaat ttgagtagaa atattattaa
     1021 atcagattta catcatttcc ctgaatatgg tacttttatc tgtgggagcc aggttcagct
     1081 ggatatttca aaaaccaact acttacgggt gattaatgct tttactcaaa ttgaagcggc
     1141 taaggcttat ttatttgcaa actctgaatt ttcgggtgcg gattgggata cgaaaatttc
     1201 aagggatatt ttctgggaag aatctatgca tggtatctat ccagagaatg ttggggtcaa
     1261 tgctagactc cttaatgatg aagctgattt ttttgactat ctaaatcatt ctgcgatttt
     1321 tactgcggaa cgtgatgggc agacctatta tttttatcct attcaggctg gggactattt
     1381 ggctacgtcc gaaatccaag catttgctct gaatggggat gaggttatta tttaccccca
     1441 agagaaggat tttgaaactc atcgtagtta ccagtaccaa gatttaacga ctcgaggaac
     1501 agttgagttt cgtagtgtgt gtacacagcc acttgatagg acttttgctt ctgcagcttt
     1561 tcacttggga ttattggtta atttagacaa gttagaagct tacttagaaa cagcaccttt
     1621 ctttaaagta tttggttatg attacaagtc tttaaggaga caattttcta agaaaaatct
     1681 tacagatgag gaagaaacta cgattattga attttccaaa gacttactcc tactagctga
     1741 ggagggacta gtggtgagaa ataaggaaga aatgacctat ttacagcctt tgagagaaga
     1801 attgagccta taatttctct tataaaggga gaattttctg aaaaatcatg atataatgga
     1861 cgagactata gataaaggat agagagtaat gacattagtt tatcaatcaa cgcgtgatgc
     1921 caacaataca gtaactgcca gccaagcaat tttgcaaggt ttggcgacgg acggcggttt
     1981 gtttacaccg gatacttatc caaaggtaga tttgaacttt gacaaattga aagatgcttc
     2041 ttaccaggaa gttgctaagc tagttttgtc agcattttta gatgacttta cagttgagga
     2101 gttggactac tgtatcaaca atgcctacga tagcaaattt gatactccag ctattgcacc
     2161 attagtgaaa ttagatgggc aatacaattt ggaacttttc catggttcaa cgattgcctt
     2221 taaggatatg gccttgtcta ttttgccata ctttatgacg actgctgcta agaaacatgg
     2281 tttggagaac aagattgtta tcttgacagc gacatctggt gacacgggga aagctgctat
     2341 ggcggggttt gcgaatgtgc ctggtactga gattatcgtc ttttatccaa aggatggtgt
     2401 cagcaagatt caagagttac aaatgaccac tcagactggc gacaatactc atgttattgc
     2461 tattgatggt aactttgacg atgcgcaaac aaatgtgaag cacatgttta acgacgtggc
     2521 tcttcgtgaa aaattgacta ccaacaagtt gcaattttca tcagctaact ctatgaacat
     2581 tggtcgtctg gtgccacaaa ttgtttatta tgtttatgct tacgctcaat tggttaagac
     2641 tggtgaaatt gtagctggtg aaaaggttaa cttcacagta ccaacaggaa actttggaaa
     2701 tatcttggct gccttttatg ccaaacaaat tggtttgcca gttggtaaat taatctgtgc
     2761 ttcaaatgac aacaatgttt tgacagactt ctttaaaaca cgtgtctatg acaaaaaacg
     2821 tgagtttaag gtaacaacca gcccatctat ggatatcttg gtatcttcaa acttggagcg
     2881 cttgattttc catcttttgg gaaataatgc tgaaaagaca actgaactta tgaatgcctt
     2941 gaacacgcaa ggacaatata agttgacaga ctttgatgca gagattttgg acctctttgc
     3001 agctgaatat gcgactgagg aagaaacggc agcagagatc aagcgtgttt gtgagttaga
     3061 ttcttatatc gaggaccctc atacagctgt tgcttcagca gtttataaaa aataccaatc
     3121 ggccactgga gatgtaacta agacagtgat tgcttcaaca gctagtccat acaagttccc
     3181 agtagttgca gtagaagctg taactggaaa agcaggttta acagactttg aagccttggc
     3241 tcaattacat gaaatctcag gcgttgcagt gccaccagca gttgatgggc ttgaaatagc
     3301 tccaattcgt cacaagacaa cagtggcagc tgctgacatg caagcagcgg ttgaggctta
     3361 tttaggactt taagacagag ggagcaaact cggttgggaa accaactgag tttcttttca
     3421 tcaggaggag agattgttta agaaaaataa agacattctt aatattgcat tgccagctat
     3481 gggtgaaaac tttttgcaga tgctaatggg aatggtggac agttatttgg ttgctcattt
     3541 aggattgata gctatttcag gggtttcagt agctggtaat attatcacca tttatcaggc
     3601 gattttcatc gctctgggag ctgctatttc cagtgttatt tcaaaaagca tagggcagaa
     3661 agaccagtcg aagttggcct atcatgtgac tgaggcgttg aagattacct tactattaag
     3721 tttcctttta ggatttttgt ccatcttcgc tgggaaagag atgataggac ttttggggac
     3781 ggagagggat gtagctgaga gtggtggact gtatctatct ttggtaggcg gatcgattgt
     3841 tctcttaggt ttaatgacta gtctaggagc cttgattcgt gcaacgcata atccacgtct
     3901 gcctctctat gttagttttt tatccaatgc cttgaatatt cttttttcaa gtctagctat
     3961 ttttgttctg gatatgggga tagctggtgt tgcttggggg acaattgtgt ctcgtttggt
     4021 tggtcttgtg attttgtggt cacaattaaa actgccttat gggaagccaa cttttggttt
     4081 agataaggaa ctgttgacct tggctttacc agcagctgga gagcgactta tgatgagggc
     4141 tggagatgta gtgatcattg ccttggtcgt ttcttttggg acggaggcag ttgctgggaa
     4201 tgcaatcgga gaagtcttga cccagtttaa ctatatgcct gcctttggcg tcgctacggc
     4261 aacggtcatg ctgttggccc gagcagttgg agaggatgat tggaaaagag ttgctagttt
     4321 gagtaaacaa accttttggc tttctctgtt cctcatgttg cccctgtcct ttagtatata
     4381 tgtcttgggt gtaccattaa ctcatctcta tacgactgat tctctagcgg tggaggctag
     4441 tgttctagtg acactgtttt cactacttgg gacccctatg acgacaggaa cagtcatcta
     4501 tacggcagtc tggcagggat taggaaatgc acgcctccct ttttatgcga caagtatagg
     4561 aatgtggtgt atccgcattg ggacaggata tctgatgggg attgtgcttg gttggggctt
     4621 gcctggtatt tgggcagggt ctctcttgga taatggtttt cgctggttat ttctacgcta
     4681 tcgttaccag cgctatatga gcttgaaagg ataggaaatg caaaaaacag cttttatttg
     4741 ggatttagac gggactttat tggactctta cgaagcgatt ttatcaggga ttgaggagac
     4801 ttttgctcag ttttctattc cttatgataa ggagaaggtg agagagttta tcttcaagta
     4861 ttcggtgcaa gatttgcttg tgcgggtggc agaagataga aatctggatg ttgaggtgct
     4921 aaatcaggtg cgtgcccaga gtctggctga gaagaatgct caggtagttt tgatgccagg
     4981 tgcgcgtgag gtgctagctt gggcagacga atcaggaatt cagcagttta tatatactca
     5041 taaggggaac aacgctttta ccattctcaa ggacttgggg gtggaatcct attttacaga
     5101 gattttaacc agtcagagtg gctttgtgcg gaagccaagt ccagaagcgg ctacctatct
     5161 gctagataag tatcagttga attctgataa tacttattat ataggggatc ggactctgga
     5221 tgtggaattt gcccagaata gtgggattca aagcatcaac tttttagagt ctacttatga
     5281 agggaatcac aggattcaag cgttagcaga tatttcccgt atttttgaga ctaagtgata
     5341 aaaagattgt gtcagttttg tgacagagac ctaacaaact atttcaagta acctagtttg
     5401 ttacaaggaa tagacagttc tgttaaatag gcccgagagg gctttttttc tacatttttt
     5461 gtgttatgat agacaggtac tcatttgaaa ggaatttgaa agaatgaaga aaagaatgtt
     5521 attagcgtca acagtagcct tgtcatttgc cccagtattg gcaactcaag cagaagaagt
     5581 tctttggact gcacgtagtg ttgagcaaat ccaaaacgat ttgactaaaa cggacaacaa
     5641 aacaagttat accgtacagt atggtgatac tttgagcacc attgcagaag ccttgggtgt
     5701 agatgtaaca gtgcttgcga atctgaacaa aatcactaat atggacttga ttttcccaga
     5761 aactgttttg acaacgactg tcaatgaagc agaagaagta acagaagttg aaatccaaac
     5821 acctcaagca gactctagtg aagaagtgac aactgcgaca gcagatttga ccactaatca
     5881 agtgaccgtt gatgatcaaa ctgttcaggt tgcagacctt tctcaaccaa ttgcagaagt
     5941 tacaaagaca gtgattgctt ctgaagaagt ggcaccatct acgggcactt ctgtcccaga
     6001 ggagcaaacg accgaaacaa ctcgcccagt tgaagaagca actcctcagg aaacgactcc
     6061 agctgagaag caggaaacac aagcaagccc tcaagctgca tcagcagtgg aagtaactac
     6121 aacaagttca gaagcaaaag aagtagcatc atcaaatgga gctacagcag cagtttctac
     6181 ttatcaacca gaagagacga aaataatttc aacaacttac gaggctccag ctgcgcccga
     6241 ttatgctgga cttgcagtag caaaatctga aaatgcaggt cttcaaccac aaacagctgc
     6301 ctttaaagaa gaaattgcta acttgtttgg cattacatcc tttagtggtt atcgtccagg
     6361 agacagtgga gatcacggaa aaggtttggc tatcgacttt atggtaccag aacgttcaga
     6421 attaggggat aagattgcgg aatatgctat tcaaaatatg gccagccgtg gcattagtta
     6481 catcatctgg aaacaacgtt tctatgctcc attcgatagc aaatatgggc cagctaacac
     6541 ttggaaccca atgccagacc gtggtagtgt gacagaaaat cactatgatc acgttcacgt
     6601 ttcaatgaat ggataaaccc gacttgataa catcattttg acgaatgaga tctagctttc
     6661 gtgatggaaa gcgattctcg ttcgtttttt ctttgtcata ctcttcgaaa atctcttcaa
     6721 accacgtcag ttttatctga aacttcaaag ctgtgctttg agcaacctgc gactagcttc
     6781 ctagtttgct ttttgatttt cattgagtat caatttgaat ggaaaatgga aagttatcat
     6841 cttgtaatga gttaagcaac attcttgcaa tctattttac tttatatcac aattaattag
     6901 tcaaatattg ataaatcaat aaaaagagag gggaagaaat gctagagatt caagatttac
     6961 tgtatcaact ccgcttgtct gagcaagcga gtacgcaatt gtttgaaaaa aggcttggga
     7021 ttagtttgac acggtatcag attttactgt ttttgctgga gcattctcct tgtaaccaaa
     7081 tggcggttca ggagcgtttg aaaattgatc aggctgcttt gacacggcat ttcaaaattt
     7141 tggaaacgga aggtttggtg gagcgtcatc gtaatcctga aaatcagcgg gaagtgttgg
     7201 tagaggctgc gaagtatgcc aaggagcagt tagtggtgaa tccccctctg caacatatca
     7261 gggttaagga agagatagaa agtatcttaa cagagtttga gagaacagaa ctcagccgtt
     7321 tattaaataa attggttttg ggtattgaaa atatagaaat ttaaggagaa atagatgtca
     7381 attattttaa caacgatcgt tgctttggag catttttaca ttttttattt ggaaagtatt
     7441 gccacgcaat cagatgcgac tagtcgtgta tttaatatgg aaaaggaaga attggctcat
     7501 ccgtcagtaa gttcattgtt caaaaatcaa ggaatttata aggctctgct aggagtcttt
     7561 ctcttgtatg tcatttattt ctcacagaat ttagaaattg tgactatttt tgtcttattt
     7621 gtgattggtg ctgcgactta cggctcttta acagcggata aaaaaattat tttgaaacaa
     7681 ggtggatcag ctattttggc cttgattagt attttactct ttaaatacac ttgaaggtcg
     7741 attctaatct cgctaatcct ttttaatcca gaataaggga aatatgttat acttgttttt
     7801 aagaaaaaag tctcattgaa ttggttttga ggagttagaa atgaaagtat tagtgacagg
     7861 ttttgagccc tttggagggg aaaagggcaa tccagctttg gaggccatta aaggtttacc
     7921 agctgaaatc catggtgctg aggtccgttg gctagaggtg ccgacagttt ttcacaaatc
     7981 tgctcaagta ttggaagaag agatgaatcg ttatcaacct gactttgtcc tttgtattgg
     8041 gcaagctggt ggaagaacta gtttgacacc tgaacgagtg accattaatc aagacgatgc
     8101 atgcatttct gataacgaag ataatcaacc gattgaccgt cccattcgcc cagatggtgc
     8161 ttcggcctac tttagtagtt tgccgattaa agcgatggtt caagctataa aaaaagaggg
     8221 cttaccggcc tctgtttcca atacggcagg gacttttgtc tgcagccatt tgatgtatca
     8281 ggctctctat ttggtagaaa agaaatctcc atatgttaag gcaggtttta tgcatattcc
     8341 ttatatgatg gaacaggtgg tgaacagacc gactactcca gctatgagtt tagtggatat
     8401 tcggcgaggg atagaagcag caatcggcgc tataatagaa catggagatc aggaactcaa
     8461 gttggtaggc ggagaaactc attgatagaa aaaagcttga ggagaaaaac cttcaagctt
     8521 ttggacgttt tcgggccaat actgctcggt aaaacataat tttagtgcat tggatataag
     8581 gtaggagtga aaaactagca atgccaaagg taatccaatt gaggaagtac caaggaagaa
     8641 gctgtaaatc taggacaaag tgctggaact tgtagccctt cataaaggaa cggctagttt
     8701 ttaggattcg tcttggtggg acctgtccta ggtctagact ataacagaga agaaattcca
     8761 cctgtgaata ggcataatac tgtggaatat agaggatatt tcctacaatg atcaagatga
     8821 gacttgcaag aaagtagagt ccaaagacca tgaggaaacg ctcggtttca actgatgaga
     8881 gatctagatt tggaaactca ggatgtaggg tgacgaattt tttggctaaa aagctactat
     8941 aaaagaggag gtaaatccca agtaaattag ggatactcca taaaaagaga tagaaacgtt
     9001 tgagaagtag ggtcaaaaag gtttgagaaa agcgctcctc atcaaagaga gctaggctgt
     9061 tttttacaga tggctccgtt ttagaatctt tcatgagtgt cagtgttgca tagacggaac
     9121 tggtcaaaag aatagtcccg ataaaggaga ctagtagagg aaagaggtag gtttgaagta
     9181 tttggccaag tatgctgaaa aatggctgtt ctaaaacagt cccgtggatc cgagataagg
     9241 gattaagaaa accagataag atgaccagca tactgggaag gatatagagg agaaagagac
     9301 ggggggtgtc agcctgaaaa tgttttgact cctgacgaat tgtttttaaa tcaatttttg
     9361 gatagttcat tctcttatta taccatagtt cttatacata gttcgtgaca gttcctactt
     9421 tttttgataa aatcatacag tgtgtccttg ggcacactgt atgaactggg actgtctttc
     9481 ccagcttcgg aggtaaaaaa tgtcagattc accaatcaaa tatcgtttga ttaagaaaga
     9541 aaaacacaca ggagctcgtc tgggagaaat catcactccc cacggtacct ttccgacacc
     9601 tatgtttatg ccagttggga cacaagccac tgtcaaaact cagtcacctg aagaattgaa
     9661 ggagatgggt tcgggaatta tcctatcaaa cacctatcat ctctggcttc gccctggaga
     9721 tgaactcatt gcacgcgctg gtggtctcca caagttcatg aattgggacc agcctatctt
     9781 gacagatagt ggtggttttc aggtttattc tttagcagat agccgtaata tcacagaaga
     9841 aggagtaacc tttaaaaatc atctaaatgg ttctaagatg ttcctatccc cagaaaaagc
     9901 catctctatt cagaataatc tgggttcaga catcatgatg tcctttgatg aatgtcctca
     9961 gttttatcaa ccttatgact acgttaagaa atcgatcgag cgtaccagcc gttgggctga
    10021 gcgtggtttg aaggctcacc gtcgtccaca tgaccaaggt ttgtttggaa ttgtgcaagg
    10081 tgcaggattt gaagaccttc gccgccaatc agctcatgat cttgtcagca tggatttctc
    10141 aggctactct atcggtggtt tggcagtggg agaaacccat gaagagatga atgcggtctt
    10201 ggactttaca actcaactgc tacctgaaaa taaacctcgt tatctgatgg gtgtgggagc
    10261 gccagatagc ttgatcgatg gggtcattcg tggggtggat atgtttgact gtgtcttacc
    10321 gactcgaatt gctcgtaacg ggacttgtat gaccagtcaa ggacgtttgg ttgtgaaaaa
    10381 tgcccagttt gctgaggact ttacgccact ggatcctgag tgtgactgct acacatgtaa
    10441 taactataca cgcgcttacc ttcgtcacct gctcaaggct gatgaaacct ttggtatccg
    10501 cttgactagc taccacaatc tttacttctt gcttaacctg atgaagcaag tgcgacaagc
    10561 catcatggat gacaatctct tggaattccg tgagtatttt gtggaaaaat atggctataa
    10621 taagtcagga cgtaatttct aaaatggaat tgatataaaa aaatcctaag ttttctctta
    10681 ggatttttct tctttttttg atagaataaa gtgtacaatg aaaggaagaa taaactcgta
    10741 tgcgcattaa atggttttcc ttgattagga ttataggttt acttttggta ctcttgtatc
    10801 acttctttca gacgatcttt cctggaggat ttttcggggt agatgtcttt ttcacatttt
    10861 caggtttcct gattacagct ctactcattg aagaattttc taagaacaat gagattgatt
    10921 tgataggatt ttttagaaga cgcttttatc ggattgtgcc acctgtggtt ttgatggtct
    10981 tggtgaccat gccttttact ttcttggttc gccaagacta tgttgctgga attggtggcc
    11041 agattgcggg cgtcttaggc tttatgacca acttctatga actcctaaca ggtgggagtt
    11101 atgaatctca gttcattcct catttgtttg ttcataattg gagtttggca gttgaggttc
    11161 actactatat tctttgggga ttggcagttt ggttcttatc caaacaagct aaatcaaatg
    11221 gtcagttgaa ggggatggtc tttctcttat ctgctgctgc cttcttgatc agtttcttct
    11281 ccatgtttat tggtagtttt ctagtgacct cttattcctc tgtttatttc tccagtttaa
    11341 ctcatgtcta tccattcttt ttgggaagta tgttagcaac tattgtaggt gttcgtcaga
    11401 cgacttccct cgtcaagcag ttggataaaa tctgggattt acgaaagact ttggtagttt
    11461 ttggaggagg ctttggtttc ctagttcttt tgactttctt tgtcaaattc acttatcttt
    11521 ttgcctatct tatcggcttc ttgcttgcca gtcttgcagc tcttgccatg attctggcgg
    11581 cgcgtgtctt acatgaaaag acacatcata tacaggagcc gaagattatc agctttttag
    11641 cggatactag ctatgcggtt tatcttttcc attggccttt ctatatcatt ttctcacagt
    11701 tgacatcaaa tcttcttgct gtattactga ctctgatttg ttcttatggc tttgccagtc
    11761 tgtcattcta tgtattggaa ccttggattg caggcaagaa cacacctatt ttacaaactc
    11821 ttcgtcccct gccttatatt cacgcaattc ttgtagcagg tacaggaatc ttgaccatca
    11881 ttgtctgcac ggtgaccttg ttggcaccac aagtgggagc gtttgagaca gacttgactg
    11941 tcaatggctt gaagcaagct gcaacaaata ttggccagac caaggtgatg gcagaacggg
    12001 cagatgcaaa cagtttggga attgctgatg gcactatgtt aattggtgac tcagtggctt
    12061 taagggcaaa tacagcacta cagacagctc ttcctggagc acagattaac gcgcaggtca
    12121 gcgtaacaac caagaccgca aatgaaatca tgctaaataa tagccagaat aaatttttac
    12181 ctaagacggt ggtcattgcg actggggtaa ataatcctga gaattacaag gatgactggg
    12241 acagtatcgt gaaaaatctt cctaagggac accatatgat tttggtgact ccttatgagg
    12301 gagataagac aaaagagacc tatgccatcg ttgagaaggc tgctgcctat atgagagaat
    12361 tggcagagaa gacaccttac attacgatag cagattggaa tcaagttgcg aaagagcatc
    12421 cagaaatttg ggctggaaca gaccaggttc atttcgggag tgagagtagc actatcgaag
    12481 caggagcaaa attgtatgca gatacgattg ccacagcttt gcagacagct caagacaagc
    12541 cggttaaatc aaaataactt gtattaaaaa agagaagagt tggagaaatc cagctctttt
    12601 aaaatatctc caaaaaatag gtctatacca tttacaaata aaaaagaaag gtttataatg
    12661 taattgacat aataaattgt agaatcaatc ttttaaggag gttaacatta tgcctaacta
    12721 tattaaagcg gatcagtttt tctacccaca cggagttcgt cgaggtggtt acttggaact
    12781 tgtggacggc aagtttggga aacatgtaga gcagattcct gaaggggctg aggtgattga
    12841 ctatacaggt tatagcattg ccccaggtct tgtggatact catattcatg gatatgcagg
    12901 tgtagatgtg atggacaaca acattgaagg tacattgcat actatgagtg aaggacttct
    12961 tagtaccggt gttaccagtt tcttacccac aactttaaca gccacttatg agcaattgct
    13021 tgcagtcact gaaaatcttg gaaaccatta taaagaagca acaggtgcta agattcgtgg
    13081 gatttattat gaaggtccat atttcacaga aacttttaag ggggcacaaa atccaactta
    13141 tatgagagac ccgggtgttg aggagtttca ttcttggcaa aaagcggcaa atggcttgct
    13201 taataaaatt gcccttgcac cagaacgtga tggggtggaa gactttgttc gtacagttac
    13261 gggcgaaggt gtgacggttg ctcttggaca ttcaaacgcg acttttgatg aagccaaaaa
    13321 agcagtcgat gctggagcga gtgtttgggt gcatgcctat aatggaatgc gtgggttgac
    13381 tcaccgtgaa ttgggtatgg ttggagccat gtaccaattg ccacatacct atgcagagtt
    13441 gatctgtgat ggtcaccacg tagatccaaa ggcttgcgaa attcttatca aacaaaaagg
    13501 aacagaaaac atcgctctta tcacagactg tatgacagct gggggattgg aagacggaga
    13561 ttatatgttg ggagaattcc cagttgtcgt tgcaaatgga actgcacgcc tcaaatcgac
    13621 aggtaacttg gcaggttcta tcctcaaact caaagatggt ttgaagaatg tggtcgaatg
    13681 gggaattgcg aatccgcatg aagcagtcat gatggccagc ttcaacccag ctaaatccgt
    13741 tcacatcgat gacgtctgtg gccaaatccg tgaaggctac gacgctgact tcatcgtatt
    13801 agataaagat ttggaattgg tagcaaccta tctagatggc gtaaaacgtt atcaagcata
    13861 agagagaagg cagaagttag agactagctt ctgctttttt atacagggta ctttactggt
    13921 aaataaaaag ttaaaaaaat cacaaaaaaa gcttgaagaa acaaagaaaa aggcttacaa
    13981 ttgaactata aatattacaa ataaaaaaac ggaggagtgc tttatgaaag cctatactta
    14041 tgttaaacca ggacttgctt cttttgttga tgtagacaaa ccagttattc gcaagccaac
    14101 agacgctatt gtgcgtattg taaaaaccac tatttgtgga acagacctcc atattatcaa
    14161 aggggatgtt cctacttgcc aaagtggtac cattcttggc cacgaaggga ttgggattgt
    14221 tgaagaagtt ggggaaggag tttccaactt caaaaaaggc gacaaggtct tgatttcttg
    14281 cgtctgtgcc tgtggtaaat gctactactg taaaaaagga atttatgccc actgtgaaga
    14341 cgaagggggc tggattttcg gtcacttgat tgatggtatg caggctgaat atctacgtgt
    14401 ccctcatgca gataatactc tttaccatac tccagaagac ttgtcagatg aagctttggt
    14461 tatgctgtca gacattctgc ctactggata tgaaattggt gtcttaaaag ggaaagtaga
    14521 acctggttgc agcgtagcca ttattggttc aggtccagtt ggattggctg ctcttttaac
    14581 agcccaattc tattcaccag ctaaattgat tatggtagac ctagacgata accgcttgga
    14641 aactgcccta tcattcggtg cgactcataa ggttaattct tcagaccctg aaaaagccat
    14701 taaagaaatt tatgatttga cagatggtcg tggtgtggat gtcgctatcg aagctgttgg
    14761 tattcctgca acatttgatt tctgtcaaaa gattatcggt gtagacggaa cggttgccaa
    14821 ctgtggtgtg catggtaaac cagttgaatt cgatttagat aaactttgga ttcgcaacat
    14881 caatgtaaca actggtttgg tatctacaaa tacgactcca caattgttga aagcacttga
    14941 aagtcataag attgaaccag aaaaattggt aactcactat ttcaaactca gtgaaattga
    15001 aaaagcctat gaagtcttca gtaaggcagc agaccaccat gccattaagg tcattatcga
    15061 aaacgatatc tcagaagcct aagtagtaaa aatatttttg tacataagta aatagaaatt
    15121 cagtcatcca tcagatggct ggatttttta tcaaaaaatt aagaaatgag catatttctt
    15181 tccttgtctg gcggaattgg ttataatata cggtacaaag gaatgaatga atatgtatcg
    15241 tgttatagaa atgtacggag attttgaacc gtggtggttc ttagaaggtt gggaagaaga
    15301 tattgtagca agtagaaaat ttgaccagta ttatgatgct ctcaaatact acaaaacttg
    15361 ctggtttaga ttggaacaag aatcccctct ttataaaagt agaagcgact tgatgaccat
    15421 tttttgggac ccggaagacc aacgctggtg tgatgaatgt gatgagtatt tacaacaata
    15481 ccattctttg gctcttttgc aggatgagca ggttatccca gacgaaaaac tacgctcagg
    15541 ctatgaaaaa caaaccagtc aggaaaggaa tcgttcttgc cgtatgaaat taaaatagag
    15601 aaaagtaact tttttggagt tgcttttttt atttttctaa cgctttgcga atagtatagg
    15661 tgaggaggta agtatggttc aagaaattgc acaagaaatc attcgttcag ctcggaaaaa
    15721 agggacgcag gatatttatt ttgtccctaa gttagacgcc tatgagcttc atatgagggt
    15781 aggagacgag cgctgtaaaa ttggtagcta tgattttgaa aagtttgcag ccgttatcag
    15841 tcactttaag tttgtggcgg gtatgaatgt gggagaaaaa agacgtagtc aactgggttc
    15901 ctgtgattat gcctatgacc agaagatggc gtctctacgt ttatctactg taggcgatta
    15961 tcgggggcat gagagtttgg ttatccgttt gttgcacgat gaggagcagg acttgcattt
    16021 ttggtttcag gatattgaag aattaggcaa gcagtacagg caacggggac tctatctttt
    16081 tgctggtccg gttgggagtg gtaagacgac cttgatgcat gaattgtcca agtcactctt
    16141 taaaggacag caagttatgt ccatcgaaga tcctgtcgaa atcaagcagg acgacatgct
    16201 tcagttgcag ttgaacgaag caatcggcct aacctatgaa aatctaatca aactttcctt
    16261 gcgtcatcga ccagatctct tgattatcgg agaaattcgt gacagcgaga cggcgcgtgc
    16321 agtggtcaga gctagtttga caggtgcgac agtcttttca accattcacg ccaagagtat
    16381 ccgaggtgtt tatgagcgtc tgctggagtt gggtgtgagt gaagaagaat tggcagttgt
    16441 tctgcaagga gtctgctacc agagattaat cgggggagga ggaatcgttg actttgcaag
    16501 cagagattat caagaacacc aagcagccaa gtggaatgag caaattgacc agcttcttaa
    16561 agatggacat atcacaagtc ttcaggctga gacggaaaaa attagctaca gctaagcaaa
    16621 aaaatatcat caccctattt aacaatctct tttctagcgg ttttcatctg gtggagacta
    16681 tctccttttt agataggagt gctttgttgg acaagcagtg tgtgatccag atgcgtgcgg
    16741 gcttgtctca ggggaaatca ttctcagaaa tgatggaaag tttgggatgt tcaagtacca
    16801 ttgtcactca gttatctcta gctgaagttc atggcaatct tcacctgagt ttgggaaaga
    16861 tagaagaata tctagacaat ctggccaagg tcaagaaaaa gttaattgaa gtagcgacct
    16921 atcctttgat tttgctgggt tttcttctct taattatgct ggggctacgg aattacctgc
    16981 tcccacaact ggatagtagc aatattgcca cccgaattat cggtaatctg ccacaaattt
    17041 ttctaggcat ggtagggctt gtttccgtgc ttgccctttt agcactaact ttttataaaa
    17101 gaagttctaa gatgagtgtc ttttctatct tagcacgcct tccctttatt ggaatctttg
    17161 tgcagaccta cttgacagcc tattatgcac gtgaatgggg gaatatgatt tcacagggaa
    17221 tggagctgac gcagattttt caaatgatgc aggaacaagg ttcccagctc tttaaagaaa
    17281 tcggtcaaga tctggctcaa accctgaaaa atggccgtga attttctcag acgataggaa
    17341 cctatccttt ctttaggaag gaattgagtc tcatcataga gtatggggaa gttaagtcca
    17401 agctgggtag tgagttggaa atctatgctg aaaaaacttg ggaagccttt tttacccgag
    17461 tcaaccgcac catgaatttg gtgcagccac tggtttttat ctttgtggca ctgattatcg
    17521 ttttacttta tgcggcaatg ctcatgccca tgtatcaaaa tatggaggta aatttttaaa
    17581 atgaaaaaaa tgatgacatt cttgaaaaaa gctaaggtta aagcttttac attggtggag
    17641 atgttagtag tcttgctgat tatcagcgtg cttttcttgc tctttgtacc taatctgacc
    17701 aagcaaaaag aagcagtcaa tgacaaagga aaagcagctg ttgttaaggt ggtggaaagc
    17761 caggcagaac tttatagctt ggataaaaat gaagatgcta ctctgagcaa gttacaagga
    17821 gatgggcgca tcacggaaga acaggttaaa gcttataatg aatactatac taaaaatgga
    17881 ggggcaaatc gtaaagtcaa tgattaaggc ctttaccatg ctggaaagtc tcttggtttt
    17941 gggacttgtg agtatccttg ccttgggctt atccagctct gtccagtcca cttttgcagc
    18001 agtagaggag cagattttct ttatggagtt tgaagaactc tatcgggaaa cccaaaaacg
    18061 cagtgtagcc agtcagcaaa agactagtct aaatttagat gggcagacgc ttagcaatgg
    18121 cagtcaaaag ttgacagttc ctaaaggaat tcaggcacca tcaggccaaa gtattacatt
    18181 tgaccgagct gggggcaatt cgtccctggc taaggttgaa tttcagacca gtaaaggagc
    18241 gattcgctat caattatatc taggaaatgg aaaaattaaa cgcattaagg aaacaaaaaa
    18301 ttagggcagt gattttactg gaagcagtag tcgctctagc tatctttgcc agcattgcga
    18361 ccctcctttt gggacaaatt caaaaaaata ggcaagagga agcaaaaatc ttgcaaaagg
    18421 aagaagtctt gagggtagct aagatggccc tgcagacggg gcaaaatcag gtaagcatca
    18481 acggagttga gattcaggta ttttctagtg aaaaaggatt ggaggtctac catggttcag
    18541 aacagttgtt ggcaatcaaa gagccataag gtcaaggctt ttaccttgtt agaatccctg
    18601 cttgccctca ttgtcatcag tgggggatta ctcctttttc aaactatgag tcagctcctc
    18661 atttcagaag ttcgctacca gcaacaaagc gagcaaaagg agtggctctt gtttgtggac
    18721 caacttgagg cagaattaga ccgttcgcag ttcgaaaaag tagaaggcaa tcgcctatac
    18781 atgaagcaag atggcaagga catcgccatc ggtaagtcaa agtcagacga ttttcgtaaa
    18841 accgatgcta gtggacgggg gtatcagcct atggtttatg gcctcaaatc agctcagatt
    18901 acagaggaca atcaactggt tcattttcgt ttccagtttc aaaaaggctt agaaagggag
    18961 ttcatctatc gtgtggaaaa agaagaaagt taaggcaggt gttctcctct acgcagtcac
    19021 catagcagcc atctttagtc ttttgttgca attttatttg aaccgacaaa tcgcccacta
    19081 tcaagactat gctttaaata aagaaaaatt ggttgctttt gccatggcta aacgaaccaa
    19141 ggataaagcc gagcaagaaa gtggggaaca ggtttttaat ctaggtcagg taagctatca
    19201 aaacaagaaa acaagcttgg tgacgactgt tcgtacgtct aagagccaat atgagtttct
    19261 gtttccttca gtcaaaatca aagaagagaa aagagataaa aaggaagagg tagcgaccga
    19321 ttcaagcgaa aaagcggaga aaaaaaatca gaagagaagc ctgaaaagaa agagaattcc
    19381 tagtcaattc aactataatg cgttgaatcc agaatagtcc actgtagttt ctagaaaatt
    19441 gctggaaatg gatgttaagc tccaattcat ttgtttatat cttatttcag tttactatac
    19501 tttgtgctaa actaaagata tgaaacatga ttttaaccac aaagcagaaa ctttcgactc
    19561 ccctaaaaat atcttcctcg caaacttggt atgtcaagca gtcgagaaac agattgatat
    19621 tttatcagac aaagtaattt tagatttcgg tggtggcacg ggtctattag ccttgcccct
    19681 agccaagcag gctaagtcag ttactcttgt agacatttct gagaaaatgt tggagcaagc
    19741 tcgtttgaaa gtggagcagc aagcaatcaa gaatatccag tttttggagc aagatttacc
    19801 gaaaaatccc ttggagaaag agtttgattg ccttgctgtt agtcgggttc ttcatcatat
    19861 gcctgatttg gatgcggctc tctcactgtt tcatcaacat ttgaaggaag atgggaaact
    19921 catcattgct gattttacca agacagaagc taatcatcat ggatttgatt tagctgaact
    19981 ggaaaacaag ctaattgagc atggtttttc atctgtgcat agtcagattc tctatagcgc
    20041 tgaagacctg tttcaaggaa atcactcaga attcttttta atagtagccc aaaaatcact
    20101 cgcctagtca gggagtgatt tttctataag gatggaaaaa agaagggaaa tttgataaga
    20161 taggaatatg gattttgaaa aaattgaaca agcttatacc tatttactag agaatgtcca
    20221 agttatccaa agtgatttgg cgaccaactt ttatgacgcc ttggtggagc aaaacagcat
    20281 ctatctggat ggtgaaactg agctaaacca ggtcaaagac aacaatcagg cccttaagcg
    20341 tttagcacta cgcaaagaag aatggctcaa gacctaccag tttctcttga tgaaggctgg
    20401 gcaaacagaa cccttgcagg ccaatcacca gtttacacca gatgccattg ctttgctttt
    20461 ggtgtttatt gtggaagagt tgtttaaaga ggaggaaatt actatcctcg aaatgggttc
    20521 tgggatggga attctaggcg ctactttctt gacctcgctt gataaaaagg tggattactt
    20581 gggaatggaa atggatgatt tgctgattga tctggcagct agcatggcag atgtaattgg
    20641 tttgcaggct ggctttgtcc aaggagatgc cgttcgtcca caaatgctca aagaaagcga
    20701 tgtggtcatc agtgacttgc ctgttggcta ttatcctgat gatgccgttg cgtcgcgcca
    20761 tcaagttgct tctagccaag aacatactta cgcccatcac ttgctcatgg aacaagggct
    20821 taagtacctc aagtcagacg gatacgctat ttttctagct ccgagtaatt tgttgaccag
    20881 tcctcaaagt gatttgttaa aagaatggct gaaagaagag gcgagtctgg ttgctatgat
    20941 tagtctgcct gaaaatctct ttgctaatgc caaacaatct aagactattt ttatcttaca
    21001 gaagaaaaat gaaatagcag tagagccttt tgtttatcca cttgctagct tgcaagatgc
    21061 aagtgtttta atgaaattta aagaaaattt tcaaaaatgg actcaaggta ctgaaatata
    21121 aaatagattt tgttataata gttgaaaacg cttaaaaagg ggtatcatgt tatgacaaaa
    21181 acaattgcaa tcaatgcagg aagttcaagt ttgaaatggc aattatactt aatgccagaa
    21241 gaaaaagtgt tagccaaagg tttgattgaa cgtatcggtt tgaaagattc aatttcaact
    21301 gtaaaatttg acggccgttc tgaacaacaa attttggata ttgaaaatca tacacaagcc
    21361 gttaaaattt tattggatga cttgattcgt ttcgatatta tcaaggctta tgacgagatt
    21421 acaggtgttg gacatcgtgt tgttgctggt ggagaatatt tcaaagaatc aacagttgtt
    21481 gagggagatg ttttagaaaa agttgaagag ttgagtttgt tggctcctct acacaacccg
    21541 gccaatgcag caggtgttcg tgccttcaag gaattgttgc cagacattac cagtgtagtt
    21601 gtttttgata cttccttcca cacaagtatg ccagagaaag cttatcgcta ccctctacca
    21661 acaaaatatt acacagaaaa caaggttcgt aaatacggtg ctcatggtac aagtcaccag
    21721 tttgtagcag gagaagctgc aaaactcttg ggacgtccat tagaagactt gaagttaatt
    21781 acctgtcata ttggtaacgg aggctcaatt acagctgtga aagccggcaa atctgtagac
    21841 acttctatgg ggttcactcc tcttggtggt attatgatgg gaacgcgtac aggggatatt
    21901 gatccagcta tcattcctta tttaatgcaa tatacagagg attttaacac accagaagat
    21961 atcagtcgtg ttcttaaccg tgaatcaggt cttttgggag tttctgctaa ttctagcgat
    22021 atgcgcgata tagaagcagc tgtagcagaa gggaatcacg aggctagctt ggcttatgaa
    22081 atgtatgttg accgtatcca aaaacatatc ggtcagtact ttgcagtgct aaatggagca
    22141 gatgccattg ttttcacagc aggtgtcggt gaaaatgcag agaatttccg tcgtgatgta
    22201 atctcaggga tttcgtggtt tggttgtgat gttgatgatg aaaagaatgt ctttggcgtt
    22261 acaggagaca tctcaacaga ggcagctaaa atccgtgtct tggttattcc aacagatgaa
    22321 gaattagtca ttgcccgtga cgttgaacgc ttgaaaaaat aagtgaaact aaaaaaatat
    22381 tcaatacaag gagttgggaa agttattttt ccagcttctt tttctgatga aattgtccaa
    22441 aaccttgcta tgattggctt ttttgaaaaa tatggtataa tagtagtaat ttaatagatg
    22501 gagttgagtt ttgaagaaaa actttcgtgt aaaaagagag aaagatttta aggcgatttt
    22561 caaggagggg acaagttttg ctaatcgcaa atttgtggtc taccaattag aaaaccagaa
    22621 aaaccatttt cgagtaggtc tatcagttag caaaaaactg gggaatgccg tcactagaaa
    22681 tcaaattaag cgacggattc ggcatattat ccagaatgca aaagggagtc tggtagaaga
    22741 tgtcgacttt gttgtcattg ctcgaaaagg agtcgaaacc ttgggatacg cagagatgga
    22801 gaaaaatcta ctccatgtat taaaattatc aaagatttac cgggaaggaa atgggagtga
    22861 aaaagaaact aaagttgact agtttgctag gactgtctct gttaatcatg acagcctgtg
    22921 cgactaatgg ggtaactagc gatattacag ccgaatcggc tgatttttgg agtaaattgg
    22981 tttacttctt tgcgaaaatc attcgctttt tatcgtttga tattagtatc ggagtgggga
    23041 ttattctctt tacggtcttg attcgtacag tcctcttgcc agtctttcag gtgcaaatgg
    23101 tggcttctag gaaaatgcag gaagctcagc cacgcattaa ggcgcttcga gaacaatatc
    23161 caggtcgaga tatggaaagc agaaccaaac tagagcagga aatgcgtaaa gtatttaaag
    23221 aaatgggtgt cagacagtca gattctcttt ggccgatttt gattcagatg ccggttattt
    23281 tggccttgtt ccaagcccta tcaagagttg actttttaaa gacaggtcat ttcttatgga
    23341 ttaaccttgg tagtgtggat acaacccttg ttcttccgat tttagcagca gtattcacct
    23401 ttttaagtac ttggttgtcc aacaaagctt tgtctgagcg aaatggcgct acgactgcga
    23461 tgatgtatgg gattccagtc ttgattttta tctttgcagt ttatgcgcca ggtggagtcg
    23521 ccctatactg gacagtgtct aatgcttatc aagtcttgca aacctatttc ttgaataatc
    23581 cattcaagat tatcgcagag cgcgaggccg tagtacaggc acaaaaagat ttggaaaata
    23641 gaaaaagaaa agccaagaaa aaggctcaga aaacgaaata aataaggagg aatctggtaa
    23701 tggtagtatt tacaggttca actgttgaag aagcaatcca gaaaggattg aaagaattag
    23761 atattccaag aatgaaggct catatcaaag tcatttctag ggagaaaaaa ggctttcttg
    23821 gtctatttgg taaaaaacca gcccaagtgg atattgaagc gattagtgaa acgactgttg
    23881 tcaaagcaaa tcaacaggta gtaaaaggcg ttccgaaaaa aatcaatgat ttgaacgagc
    23941 ctgtgaagac ggttagtgaa gaaaccgttg accttggtca tgtggttgat gctattaaaa
    24001 aaatagagga agaaggtcaa ggtatttctg atgaagtcaa ggctgaaatc ttaaaacatg
    24061 aaagacatgc cagcactatc ttagaagaaa ctggtcacat tgagatttta aatgaacttc
    24121 aaatcgagga agcgatgagg gaagaagcag gcgctgatga ccttgaaact gagcaagatc
    24181 aaactgaaaa tcaagacttg aaagagatgg gcttgaaggt cgagcaaagt tatgatattg
    24241 cccaggtggc tacggatgtg actgcctatg ttcaagcgat tgtggatgac atggatgttg
    24301 aagctacact ttcaaatgat tataaccgtc gtagcatcaa tctacaaatt gacaccaacg
    24361 aaccaggtcg tattatcggc taccatggta aagtcttgaa ggccttgcaa ctgttggctc
    24421 aaaattatct ttacaaccgc tattccaaaa ccttctacgt tacaatcaat gtcaatgatt
    24481 atgtcgaaca ccgtgcagaa gtcttgcaga cctatgcgca aaaattggcg attcgtgttt
    24541 tagaagaagg acgtagtcat caaacagatc caatgtcgaa tagtgaacgc aagattatcc
    24601 accgtattat ttcacgtatg gatggcgtga ccagttactc tgaaggtgat gagccaaatc
    24661 gctatgtcgt tgtagataca gaataagtaa aatcagggtt gacctgattt tttgctagct
    24721 agaggaggtt aaactgatgt tgaataagat aagagactat ttagactttg ctggtttgca
    24781 gtaccgtaat cctgataaag cgggagcaga gcgagagaag atgctggcat tccgccacaa
    24841 aggacaagag gcccgaaagg cttttacaga actggccaaa gcctttcaag caagccatcc
    24901 agaatggcaa ctccaacaga ctagtcagtg gatgaatcag gcccagcgtt tgagaccaca
    24961 tttttgggtt tatctacaga gagacggaca agtgacagaa cctatgatgg ccttacgttt
    25021 gtatgggaca tctactgact ttggaatttc tttggaagtc agtttcatcg aacgtaagaa
    25081 ggatgagcaa acactgggca agcaggccaa agttttagac attccaaccg ttaaagggat
    25141 ttattatcta acctactcta atggtcaaag tcaacggtgg gaggcgaatg aagaaaagcg
    25201 tcgtacttta cgcgagaagg tgagaagtca agaagttcga aaagttttag tgaaggtaga
    25261 tgttcctatg acagaaaatt cgtctgaaga agaaatcgta gaaggcttat tgaagtctta
    25321 ttctaaaatt cttccctatt atctagctac gagaaaataa gataatttgt aaaacatcat
    25381 aaatcataca gtccaagagt gaacagtccg ctgtgtaatt cttggtcttt ttgtttgcgc
    25441 tttcgcatta tataataaac ttacaaaaac aattcaaaag gagaacaatt atggaagtcg
    25501 tttcaagtgt tctaaattgg ttttctagca atattttgca gaatcccgca tttttcgtag
    25561 gtttattggt gttgatagga tatgcacttt tgaaaaaacc tgcccatgac gttttttcag
    25621 ggtttgttaa agcaacagta gggtatatgt tgcttaacgt gggtgctggt ggtttggtta
    25681 caacctttcg tccaatctta gcagctctta actacaaatt ccaaattggt gcagcggtta
    25741 tcgaccctta ctttggactt gctgcagcaa acaacaaaat tgcagcagag tttccagatt
    25801 ttgttggaac tgcaactaca gctctattga ttggttttgg aataaatatc ttgctcgtag
    25861 ctcttcgaaa gattacgaag gtaagaaccc tctttattac tggtcacatc atggtacaac
    25921 aagctgcaac agtatctctt atggttctat tcttagtacc acaattgcgc aatgcttacg
    25981 gtacagcagc gattggtatc atctgtggac tttactgggc agttagttca aatatgactg
    26041 ttgaggcaac tcaacgcttg actggtggtg gcggatttgc gattggtcac caacagcaat
    26101 ttgcaatctg gtttgtagat aaagtagcag gacgctttgg taagaaagaa gaaagtttag
    26161 acaatcttaa attacctaag ttcctctcaa tcttccacga tacagttgtt gcatctgcta
    26221 ccttgatgct cgtattcttc ggggccattc ttttaatctt gggtccagac attatgtcta
    26281 ataaagaagt catcacttca ggaactctat tcaatcctgc taaacaagat ttctttatgt
    26341 acattatcca aacagccttt accttctcag tttacttgtt cgttttgatg caaggtgtcc
    26401 gaatgttcgt atctgagttg acaaacgcct tccaaggtat ttcaaacaaa ttgttgccag
    26461 gttcattccc agcggttgac gttgcagctt cttatggatt tggttctcca aatgctgtct
    26521 tgtcaggatt tacctttggt ttgattggtc aattgattac aattgtcttg ctcatcgtct
    26581 ttaaaaatcc gattcttatt attacaggat ttgtaccagt gttctttgac aatgcagcca
    26641 ttgcggtcta cgctgataaa cgcggcggat ggaaagcggc tgttatcctt tcctttatat
    26701 caggtgtcct tcaagttgct ctaggagctc tttgtgtggc ccttctcgat ttggcatctt
    26761 atggtggcta ccatggaaat atcgactttg aattcccatg gcttggattt ggatatatct
    26821 tcaaatacct tggtattgtt ggttatgtac ttgtgtgtct cttcttgctt gttattcctc
    26881 aacttcaatt tgccaaagca aaagataaag agaaatatta caacggtgaa gttcaagaag
    26941 aagcttagta tctagaaaag gagaaataaa atggttaaag tattagcagc gtgcggaaat
    27001 ggaatgggtt catcaatggt tatcaagatg aaggttgaaa atgctctccg taagcttaat
    27061 caaacagatt ttacagtcaa ttcatgcagt gtcggtgaag ctaaaggttt agcagtagga
    27121 tatgacatcg taatcgcttc tcttcatttg attcaagaat tggaagggcg aactaatggg
    27181 aagttaattg gacttgataa cttgatggat gataaagaaa tcaccgaaaa actcagtcaa
    27241 gcactacagt aaaaggttgg agggggctgg acagaaactg agagttatcg tttctgtcct
    27301 tctccctctt taaataaagg aggcagatat gaatttaaaa caagctttaa ttgacaatga
    27361 ctcgatccga ctaggtttag aggctaacaa ttggaaagaa gcagtcaagg tagcagtaga
    27421 tcccttaatt gaaagtgggg caattttgcc agagtattac gatgctatca ttgaatcgac
    27481 tgaagagtat gggccttact atatcttgat gccaggtatg gctatgcccc acgctagacc
    27541 tgaagctggt gtgcaaagtg atgccttttc attgattacc ttacaaaatc ctgttgtatt
    27601 ttcagatggg aaagaggtat ctgttttgtt ggcactagca gcaacaagct caaaaattca
    27661 cacaagtgta gccattccac aaattattgc cctgtttgaa ttagaagatt ctattgcacg
    27721 tttacaggct tgccagacta aagaagatgt cttggctatg attgaagaat ctaaggatag
    27781 cccttatctc gaaggattgg atttggaaag ttagaaagag gaataaagaa atgacaaaaa
    27841 gaatacctaa tttacaagtt gcattagacc attcagactt gcaaggagcg attaaagcag
    27901 ctgtttctgt tggtcaggaa gtagatatta tcgaagctgg aactgtttgc ttgcttcaag
    27961 ttggaagtga actggctgaa gtcttgcgta gccttttccc agataagatt attgtggcag
    28021 acacaaaatg tgctgatgct ggtggaacag ttgctaaaaa taatgcggtt cgtggagcag
    28081 actggatgac ttgtatctgt tgtgcaacca tccctactat ggaagcagct ctaaaggcta
    28141 tcaagactga acgaggagaa cgaggcgaaa tccagatcga gctttatggc gattggactt
    28201 ttgaacaagc tcagctttgg ctagatgcag gtatctcaca agctatttat caccaatctc
    28261 gtgatgctct tcttgctggt gaaacttggg gtgaaaaaga ccttaataag gttaaaaaac
    28321 tcattgacat gggcttccgt gtatctgtaa caggtggtct agatgtagat actctcaaac
    28381 tctttgaagg tgttgatgtc tttaccttta tcgcaggtcg tggaattaca gaggctgcgg
    28441 atccagcagg agcagcgcgt gccttcaagg atgaaatcaa acgaatttgg gggtaaatca
    28501 tggtacgtcc aattggaatt tatgaaaagg caaccccaac acactttact tggctagaac
    28561 gtttaaattt tgccaaggag ttaggctttg attttgtcga gatgtctatt gacgaacgtg
    28621 acgagcgttt agcaagactt gactggagta aggaagaacg cttggaagtt gtcaaagcaa
    28681 tctatgaaac tggtgttcgt attccttcta tctgtttttc aggccatcgt cgctacccat
    28741 tgggttcaaa agatccagtt ctagaggaaa aatctctaga actcatgaaa aaatgtatcg
    28801 aattagctca agacttggga gttcgtacga ttcaattagc tggttacgat gtttactatg
    28861 aggaaaagtc accccagaca cgccaacgtt ttatcaaaaa tttgagaaaa gcctgtgact
    28921 gggctgaaga agctcaggtg gtacttgcta ttgaaattat ggatgatcct ttcatcaata
    28981 gcatcgaaaa atatttggct atagaaaaag agattgactc tcccttcctc tttgtatatc
    29041 cagatattgg taatgtgtct gcatggcata atgatatcta tagtgagttt tatcttggtc
    29101 atcatgccat cgcagctctc catctcaagg atacttatgc agtgacagaa agttcaaagg
    29161 gccagttccg agatgtacct ttcgggcaag gttgtgtcaa atgggaagaa gctttcgata
    29221 ttttaaagga aaccaattat aatggacctt tcctaatcga aatgtggtct gaaaattgtg
    29281 aaacagtaga agaaacacgc gcagccattc aagaggcgca agcttttctc tatccactca
    29341 ttaagaaagc aggtttgatg taagatgaat caagtaatca atgctatgcg taaacgagtc
    29401 tgtgatgcca atcaatcatt gccaaaacat ggacttgtca aatttacctg ggggaatgta
    29461 tctgaagtta atcgcgaact cggtgtcatt gttatcaaac catcaggcgt ggattatgac
    29521 gaattgacac ctgaaaacat ggtagtgact gatctagatg gtaagatcct agaaggggat
    29581 ttaagaccat cttccgacct cccaactcat gtgcaattat ataaggcttg gtcagaaatt
    29641 ggtagtgtgg ttcacaccca ttcgacagaa gctgttggtt gggctcaggc aggtcgtgat
    29701 attcctttct acggaacaac ccatgcagat tatttctacg gttcaatccc ttgcgcccgt
    29761 agtttgacca aggacgaagt agaagtggcc tatgaaaaag atactggcct ggttatcgta
    29821 gaagagtttg aacatcgcgg acttaacccg gttgaagtac caggaattgt tgtacgcaat
    29881 cacggtccat tcacctgggg caaaaatcca gagaatgctg tttatcactc tgtcgtacta
    29941 gaggaagtat caaagatgaa tcgctttaca gaacaaatca atccaagagt tggacctgct
    30001 ccccagtaca tactagaaaa acactaccaa cgtaaacatg gaccaaatgc ttattatggt
    30061 caaaagtaag aacgatgaag gaggagaaaa agataaattt agctcctctt tttacatttg
    30121 atttttattg agagtaaagt tggaattgaa gtaattttaa aagatttttt agaaatagcg
    30181 cttgatatat atatggtaaa ataaaaagaa ttgctgtgat atcaatagat ttgggggatt
    30241 ttttaatatg gtactggata aggcaagttg tgatttgctt caatatttga tggatcaaga
    30301 aacgtccaaa acgattatgg cgatttcgaa agatttgaaa gagtcaagaa ggaaaattta
    30361 ttatcacatt gacaaaatca atgctgctct ggttgacgag gcgcttcaca tcattagtat
    30421 tccacgaatt ggtattcact taacggaaga gcagagagat gcttgttgta aactattatc
    30481 ggaagtagat tcgtacgatt atatcatgag tgcgcatgaa cgtatgatga taatgttact
    30541 atggataggt atttctaaag aacgtattac gattgaaaaa ttgatagagt taacagaggt
    30601 atctaggaat actgttctca atgatttgaa tagtattcgt tatcaactaa ctttggaaca
    30661 atatcaggtg atcttgcaag tgagcaagtc acagggatac caccttcatg cccaccctct
    30721 taataaaatt cagtatcttc aatcgcttct atatcatatt tttatggaag aaaatgccac
    30781 ttttgtatct attttagaag ataagatgaa agagaggtta gatgatgagt gtttgctttc
    30841 tgttgaaatg aaccaatttt ttaaggaaca ggttccttta gttgaacaag atttagggaa
    30901 gaaaataaac catcatgaaa taacttttat gttgcaggtt ctaccttatt tgctgttaag
    30961 ctgtcataat gttgaacagt atcaagaaag acatcaggat atagagaaag aattttcttt
    31021 gataagaaaa agaatagagt atcaggtgtc taagaaatta ggagaacggt tgtttcaaaa
    31081 gtttgaaatt tctttgtcag gacttgaagt ttctcttgta gctgttctcc tcctctccta
    31141 tcgtaaggat ttggatattc atgcagaaag tgatgatttt cggcaattaa aacttgcttt
    31201 agaagaattt atctggtatt ttgaatcaca aatccgaatg gagattgaga acaaggatga
    31261 tttgttacga aatttgatga tccactgtaa agccttgtta tttagaaaga cttacggtat
    31321 tttttctaaa aatcctctaa caaaacaaat tcgatccaag tatggagaat tatttttagt
    31381 cactagaaaa tctgcggaaa ttttagaagg agcatggttt attcggctaa cagacgatga
    31441 tattgcctat ttgacgattc atattggagg atttttaaag tatacaccat cgtctcaaaa
    31501 aaatatgaaa aaagtttatc tcgtttgtga tgaaggtgtt gcggtttcga gacttttgct
    31561 gaaacaatgc aaactttatt ttccaaatga gcaaattgac actgtattta caacagaaca
    31621 atttaagagt gtggaagata ttgcacaagt tgatgtagtg attactacta atgatgattt
    31681 ggatagcaga tttccgattt taagggttaa tcctatcctt gaagcagaag atattttgaa
    31741 aatgctagac tatcttaaac acaatatatt tcgtaataag agcaaaagtt tcagtgaaaa
    31801 tctttctagt cttatttcgt cttatattgt agacagcaag ttggctagta agttccaaga
    31861 agaggttcaa acacttataa ataaagaaat agtagttcaa gcttttttgg aagatatttg
    31921 aaggacagtc caatgatgaa cacaaacctg tgtttttctt ggtctttttt agtgttttga
    31981 agggtggtat actaatctca aagataacaa ttatatccaa aggaggcaac atatgccaaa
    32041 cgtcaaagaa attacaagag agtcatggat tttagccact ttcccagagt ggggaacatg
    32101 gttgaacgaa gaaatcgaag aagaagtcgt acctgaaggc aactttgcca tgtggtggct
    32161 aggcaactgt ggtacttgga ttaagacacc agctggtgct aacgttgtca tggacctttg
    32221 gtcaaaccgt ggaaaatcaa ccaaaaaagt gaaagatatg gttcgtgggc accaaatggc
    32281 aaatatggca ggtgttcgta agttgcaacc aaacttgcgt gttcagccaa tggttatcga
    32341 tccatttgct atcaacgaac tagactatta cttagtttca cacttccaca gtgatcatat
    32401 cgacccatac acagctgcag caattctcaa caatcctaag ttagagcatg ttaagtttat
    32461 cggtccttac cactgtggac gaatctggga aggatggggt gttccaaaag aacgtatcat
    32521 cgttgttaaa ccaggtgaca ctatcgaatt aaaagatatg aagattcatg cagtagaatc
    32581 atttgaccgt acttgcttgg taactctccc agtgaacggt gctgatgaga caggcggtga
    32641 acttgctggc ttggctgtta cagatgaaga aatggctcaa aaggctgtta actatatctt
    32701 tgaaacacca ggtggaacca tctatcatgg tgcagattct cacttctcaa actattttgc
    32761 aaaacatggt aaagacttta aaattgatgt tgctttgaat aactatggtg aaaatccggt
    32821 aggtatccaa gacaaaatga catctatcga tcttcttcgt atggcagaaa atctgcgtac
    32881 caaagtcatt atcccagttc actatgatat ctggtctaac ttcatggctt ctactaatga
    32941 gattctagaa ctttggaaaa tgcgaaaaga tcgcttgcaa tacgatttcc atccatttat
    33001 ctgggaagtt ggcggtaagt acacttatcc tcaagatcaa cacttagtag aataccatca
    33061 tccacgtggt tttgatgatt gttttgaaca agactctaac attcaattta aagctttgct
    33121 ataatataaa aatattcttt ggaggttatc cgtatgttgc atgatacgga taattttcat
    33181 ataatagtaa aatagaatgt gtgattcaat aatcacctca aatagaaagg aaattctatg
    33241 tcaaatctat ctgttaatgc aattcgtttt ctaggtattg acgccattaa taaagccaac
    33301 tcaggtcatc caggtgtggt tatgggagcg gctccgatgg cttacagcct ctttacaaaa
    33361 caacttcata tcaatccagc tcaaccaaat tggattaacc gcgaccgctt tattctttca
    33421 gcaggtcatg gttcaatgct cctttatgct cttcttcacc tttctggttt tgaagatgtc
    33481 agcatggatg agattaagag tttccgtcaa tggggttcaa aaacaccagg tcacccagaa
    33541 tttggtcata cggcagggat tgatgctacg acaggtcctc tagggcaagg gatttcaact
    33601 gctactggtt ttgcccaagc agaacgtttc ttggcagcca aatataaccg tgaaggctac
    33661 aatatctttg accactatac ttacgttatc tgtggagacg gagacttgat ggaaggtgtc
    33721 tcaagcgagg cagcttcata cgcaggtttg caaaaacttg ataagttggt tgttctttat
    33781 gattcaaatg atatcaactt ggatggtgag acaaaggatt cctttacaga aagtgttcgt
    33841 gaccgttaca atgcctacgg ttggcatact gccttggttg aaaatggaac agacttggaa
    33901 gccatccatg ctgctatcga aacagcaaaa gcttcaggca agccatcttt aattgaagtg
    33961 aagacggtta ttggatatgg ttctccaaac aaacaaggaa ctaatgctgt acacggcgcc
    34021 cctcttggag cagatgaaac cgcatcaact cgccaagccc tcggttggga ctacgaacca
    34081 tttgaaattc cagaacaagt atatgctgat ttcaaagaac atgttgcaga ccgtggcgca
    34141 tcagcttatc aagcttggac taaattagtt gcagattata aagaagctca tccagaactg
    34201 gctgcagaag tagaagccat catcgacgga cgtgatccag tcgaagtgac tccagcagac
    34261 ttcccagctt tagaaaatgg tttttctcaa gcaactcgta attcaagtca agatgccttg
    34321 aatgttgtgg cagctaaatt accaaccttc ctaggtggat cagctgacct tgctcactca
    34381 aacatgactt atatcaagac ggatggactt caagacgata ccaatcgctt gaatcgtaac
    34441 attcagtttg gtgttcgtga atttgcaatg ggaacaatct tgaacgggat ggcccttcat
    34501 ggtggacttc gtgtatacgg tggaactttc ttcgtcttct ctgactatgt gaaggcagct
    34561 gtccgcttgt cagccttaca aggacttcct gtgacttatg tctttaccca tgattcaatc
    34621 gcagttgggg aagatggtcc gactcacgaa ccagttgagc atttagcagg tcttcgtgct
    34681 atgccaaatc taaatgtttt ccgtccagca gatgcgcgtg aaacgcaagc agcttggtac
    34741 cttgcagtga caagtgagaa aacaccaact gcccttgtct tgacacgtca aaacttgact
    34801 gttgaagatg gaacagactt cgacaaggtt gctaaaggtg cttatgttgt atatgaaaat
    34861 gcagccgact ttgataccat cttgattgcg acaggttcag aggttaatct tgctgtctca
    34921 gctgctaaag aattggctag tcaaggcgaa aaaatccgcg tagtcagcat gccatctaca
    34981 gatgtctttg ataaacaaga tgcagcttac aaggaagaga ttcttccaaa tgcagtccgc
    35041 cgtcgtgttg cagtcgaaat gggtgcaagt caaaactggt acaaatatgt tggtctcgat
    35101 ggtgccgttc taggtattga taccttcgga gcctctgccc cagcaccaaa agtattggca
    35161 gaatatggct ttactgtaga aaatcttgta aaaattgttc gaaacttgaa ataatcctaa
    35221 aaatcagggc gtaagctctg gtttttctta ccagaaaagt aaggtacaat cttgtaaaag
    35281 tagctgaaat ttgatatagt agtcctatgt aaaagacaaa ggagaaaaca atgaatccaa
    35341 atattacttt tttaatcatg cttgtaggta tgatggcctt gatgttcttt atgcaacgtt
    35401 ctcaaaagaa acaagctcaa aagcgtatgg aaagcttaaa taaactacaa aaaggctatg
    35461 aagtgattac aatcggtgga ctttatggaa cagtcgatga agtagatacg gagaagggaa
    35521 caatagttct tgacgtagat ggagtttact tgacttttga actagctgct atcaagacag
    35581 tattacagct gaaagaaaca gcttcactag aaggcgcaat tgaaaaataa gacgggatca
    35641 cgaactcccg tttttctata aaagaaagga aatgggatga aaaaattagt ctttgtctgt
    35701 ctgggaaata tttgccgtag ccctatggcc gagtttgtta tgaaatcaat gacagataac
    35761 tacgaaatcc aaagtcgagc aacttcctct tgggaacatg gcaatccgat tcataagggg
    35821 actcagggaa tttttcaaga gtatgagatt ccttatgaca agaacaagac atcgcttcag
    35881 attagtaagg aagattttga agcctttgat tatattatcg gaatggacgc ttcaaatatt
    35941 tctgacttat gtcagatgtg tccagtagac tgtcaagata agatttactc attttcatct
    36001 gaaagtgttc cagacccttg gtatacagga gattttgaag aaacctatcg acgtgttcaa
    36061 gagggctgtc aagcttggtt agaacgttta gaaaaggaga gttaaggtgg aaaatcttac
    36121 gaatttttac gaaaagtatc gtgtctatct gactcgtcca cgtttagagc ttttggcagt
    36181 agttaccatt gttttttgtg ctgtactcgt cttttttcta aatattccag gaaaaggtgt
    36241 cttaaaactc gataatggaa cgattgttta tgatggcagt cttgtccgtg gtaaaatgaa
    36301 tggccaaggt accattacct tccaaaatgg agaccaatat acaggtggct tcaacaatgg
    36361 agccttcaac ggaaaaggta cctttcaatc taaagaaggc tggacctacg aaggtgattt
    36421 tgtaaatggt caggctgaag gaaaagggaa actaacaaca gaacaagaag tcgtttatga
    36481 aggaactttt aaacaaggcg tttttcaaca aaaataaagc ctccttatca aaggaggtat
    36541 tattagaatc acaaggtaag cgcttacctg taaatccctt tctttccaaa tccctcttcc
    36601 aagcaagttt gtgaaataaa aaatatttga aataaatttc acaaacttca aagataaaac
    36661 ttgataagaa aagaaaatga gaaaagtttc acaagagttt aaaaattctt tgtgaaatgt
    36721 ttatgaaact gtttacaaag ttgaaataat gcgttataat aaacttgtga aaaaattaac
    36781 aaaggatatc gttccttgaa agctatggag gaaaatatgg ctgataaaaa aactgtgaca
    36841 ccagaggaaa agaaactcgt tgctgaaaaa cacgtagatg agttggttca aaaagctcta
    36901 gttgcccttg aagaaatgcg taaattggat caagaacaag ttgactacat cgttgccaaa
    36961 gcatcagtag cagctttgga tgcccacgga gaattggctt tacatgcctt tgaagaaaca
    37021 ggacgtggtg tatttgaaga caaagcaact aagaacttgt ttgcttgtga acacgtagta
    37081 aacaatatgc gccacactaa gacagttggc gttatcgaag aagacgatgt aacaggattg
    37141 actcttattg ctgaaccagt tggtgttgtt tgtggtatta ctccaacaac aaacccaaca
    37201 tcaacagcaa tcttcaaatc attgatttca ttgaagacac gtaacccaat cgtctttgcc
    37261 ttccatccat cagcacaaga atcatctgct catgcagctc gtatcgtccg cgatgcagct
    37321 atcgcagctg gtgctcctga aaactgtgtg caatggatta ctcaaccatc tatggaagca
    37381 acaagtgccc ttatgaacca cgaaggtgtt gcgacaatcc ttgcaacagg tggtaatgcc
    37441 atggttaagg cggcttattc atgtggtaaa ccagctcttg gggtaggtgc cggaaacgtt
    37501 ccagcttatg ttgaaaaatc agcaaacatt cgtcaagcag cacacgatat cgtcatgtct
    37561 aaatcatttg ataacggtat ggtctgtgca tctgaacaag cagttatcat tgataaagaa
    37621 atttacgatg aatttgtagc agagttcaaa tcttaccaca cttactttgt aaacaaaaaa
    37681 gaaaaagctc ttcttgaaga gttctgcttc ggcgtcaaag caaacagcaa aaactgtgct
    37741 ggtgcaaaat tgaacgctga catcgttggt aaaccagcaa cttggattgc agaacaagca
    37801 ggatttacag ttccagaagg aacaaacatt cttgctgcag aatgtaaaga agttggcgaa
    37861 aatgagccat tgactcgtga aaaattgtca ccagttattg cagttttgaa atctgaaagc
    37921 cgtgaagatg gtattactaa ggctcgtcaa atggttgaat ttaacggtct tggacactca
    37981 gcagctatcc acacagctga cgaagaattg actaaagaat ttggtaaagc tgttaaagct
    38041 attcgtgtta tctgtaactc accttctact tttggtggta tcggggacgt ttacaatgcc
    38101 ttcttgccat cattgacact tggatgtggt tcttacggac gcaactcagt tggggataac
    38161 gttagtgcca ttaacctctt gaatatcaaa aaagtcggaa gacggagaaa taacatgcaa
    38221 tggatgaaac ttccttcaaa aacatacttt gaacgtgatt caattcaata ccttcaaaaa
    38281 tgtcgtgacg ttgaacgtgt catgatcgtt actgaccatg ccatggtaga gcttggtttc
    38341 cttgatcgta tcatcgaaca actggacctt cgtcgcaata aggttgttta ccaaatcttt
    38401 gcggatgtag aaccggatcc agatatcaca actgtaaacc gtggtactga gattatgcgt
    38461 gccttcaaac cagataccat catcgcactc ggtggtgggt ctccaatgga tgctgccaaa
    38521 gtaatgtggc tcttctacga gcaaccagaa gtggacttcc gtgaccttgt ccaaaaattc
    38581 atggatatcc gtaaacgtgc cttcaagttc ccattgcttg gtaagaagac taaattcatc
    38641 gcgattccaa ctacatctgg tacaggatct gaagtaacac catttgccgt tatctctgat
    38701 aaagcaaaca accgtaaata cccaatcgct gactactcat tgacaccaac tgtggcaatc
    38761 gtagatcctg ctttggtatt gacagttcca ggatttgttg ctgctgatac tggtatggac
    38821 gtattgactc acgcgacaga agcatacgta tcacaaatgg ctagtgacta cactgatggt
    38881 ttagcacttc aagccattaa attggtcttt gaaaatctcg aaagctcagt taagaatgca
    38941 gacttccact cacgtgagaa aatgcataac gcttcaacaa tcgctggtat ggcctttgcc
    39001 aatgccttcc taggtatttc tcactcaatg gcccataaga ttggtgcgca attccacaca
    39061 atccacggtc gtacaaatgc tatcttgctt ccatacgtta tccgttacaa cggtacacgt
    39121 ccagctaaga cagcaacatg gcctaagtac aactactacc gtgcagatga aaaataccaa
    39181 gatatcgcac gcatgcttgg acttccagct tctactccag aagaaggggt tgaatcttac
    39241 gcaaaagctg tctacgaact cggtgaacgt attgggatcc aaatgaattt tagagaccaa
    39301 ggaattgacg aaaaagaatg gaaagaacat tctcgtgaat tagccttcct ggcttatgaa
    39361 gaccaatgtt caccagctaa cccacgtctt ccaatggtag accatatgca agaaatcatc
    39421 gaagatgcat actatggcta caaagaaaga ccaggacgcc gtaaataatt gtttatcagt
    39481 ctagaagcaa gacaaaaact caatttgagg gaaagatcca gtaatttttc tatgataaaa
    39541 ggcatcctat caaggttttt gaacacctga taggatgcct ttttatgata ttgaggcctt
    39601 tttgcccttt ttgaaaaact agaatagaaa caaaatatat aatagattga aactggaata
    39661 gtacacttct gcttctaaaa cattgttaga attcgatttg actgtcctga tcgatttgtc
    39721 ctattcctat ttcattttat tatataaaaa atat
//
LOCUS       DBEJAQ010000015        39439 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.015, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000015 DBEJAQ010000000
VERSION     DBEJAQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.015"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(300..1280)
                     /gene="dusB"
                     /locus_tag="ACL297_000842"
     CDS             complement(300..1280)
                     /gene="dusB"
                     /locus_tag="ACL297_000842"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGR0071127.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1267..2139)
                     /gene="hslO"
                     /locus_tag="ACL297_000843"
     CDS             complement(1267..2139)
                     /gene="hslO"
                     /locus_tag="ACL297_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGR0071128.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2304..3731
                     /locus_tag="ACL297_000844"
     CDS             2304..3731
                     /locus_tag="ACL297_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0071129.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            3890..5398
                     /gene="glpK"
                     /locus_tag="ACL297_000845"
     CDS             3890..5398
                     /gene="glpK"
                     /locus_tag="ACL297_000845"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGR0071130.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5440..7266
                     /gene="glpO"
                     /locus_tag="ACL297_000846"
     CDS             5440..7266
                     /gene="glpO"
                     /locus_tag="ACL297_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HGR0071131.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     STVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7336..8040
                     /locus_tag="ACL297_000847"
     CDS             7336..8040
                     /locus_tag="ACL297_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGR0071132.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8337..8540
                     /locus_tag="ACL297_000848"
     CDS             8337..8540
                     /locus_tag="ACL297_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071133.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8627..9235)
                     /locus_tag="ACL297_000849"
     CDS             complement(8627..9235)
                     /locus_tag="ACL297_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071134.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            9367..9558
                     /locus_tag="ACL297_000850"
     CDS             9367..9558
                     /locus_tag="ACL297_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071135.1"
                     /translation="MLNAKIFYIFLCLIVIETTGLLLLKRGLEMGYLDTEAIILAVFS
                     FAFYNLCSFAWVCSTIKNK"
     gene            10370..11647
                     /gene="ltrA"
                     /locus_tag="ACL297_000851"
     CDS             10370..11647
                     /gene="ltrA"
                     /locus_tag="ACL297_000851"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGR0071136.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGISTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVVSEAAAKRMMYSVSRFIEKRLGLKANMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            12103..13281
                     /locus_tag="ACL297_000852"
     CDS             12103..13281
                     /locus_tag="ACL297_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ryptide export MFS transporter"
                     /protein_id="HGR0071137.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            13595..13726
                     /locus_tag="ACL297_000853"
     CDS             13595..13726
                     /locus_tag="ACL297_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HGR0071138.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            13747..15297
                     /gene="dltA"
                     /locus_tag="ACL297_000854"
     CDS             13747..15297
                     /gene="dltA"
                     /locus_tag="ACL297_000854"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HGR0071139.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNAHIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFENLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            15294..16538
                     /gene="dltB"
                     /locus_tag="ACL297_000855"
     CDS             15294..16538
                     /gene="dltB"
                     /locus_tag="ACL297_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGR0071140.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            16552..16791
                     /gene="dltC"
                     /locus_tag="ACL297_000856"
     CDS             16552..16791
                     /gene="dltC"
                     /locus_tag="ACL297_000856"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGR0071141.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            16784..18052
                     /gene="dltD"
                     /locus_tag="ACL297_000857"
     CDS             16784..18052
                     /gene="dltD"
                     /locus_tag="ACL297_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGR0071142.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLQQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            18207..18647
                     /gene="adcR"
                     /locus_tag="ACL297_000858"
     CDS             18207..18647
                     /gene="adcR"
                     /locus_tag="ACL297_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HGR0071143.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            18647..19351
                     /locus_tag="ACL297_000859"
     CDS             18647..19351
                     /locus_tag="ACL297_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGR0071144.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            19344..20150
                     /locus_tag="ACL297_000860"
     CDS             19344..20150
                     /locus_tag="ACL297_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HGR0071145.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAVVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            20160..21665
                     /gene="adcA"
                     /locus_tag="ACL297_000861"
     CDS             20160..21665
                     /gene="adcA"
                     /locus_tag="ACL297_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HGR0071146.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYTEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(22836..23609)
                     /locus_tag="ACL297_000862"
     CDS             complement(22836..23609)
                     /locus_tag="ACL297_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGR0071147.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            23785..25188
                     /locus_tag="ACL297_000863"
     CDS             23785..25188
                     /locus_tag="ACL297_000863"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HGR0071148.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            25303..25941
                     /locus_tag="ACL297_000864"
     CDS             25303..25941
                     /locus_tag="ACL297_000864"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGR0071149.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            25953..26393
                     /locus_tag="ACL297_000865"
     CDS             25953..26393
                     /locus_tag="ACL297_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HGR0071150.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            26386..26817
                     /locus_tag="ACL297_000866"
     CDS             26386..26817
                     /locus_tag="ACL297_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGR0071151.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            26808..27278
                     /locus_tag="ACL297_000867"
     CDS             26808..27278
                     /locus_tag="ACL297_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0071152.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            27303..28076
                     /locus_tag="ACL297_000868"
     CDS             27303..28076
                     /locus_tag="ACL297_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGR0071153.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            28073..28873
                     /locus_tag="ACL297_000869"
     CDS             28073..28873
                     /locus_tag="ACL297_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGR0071154.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            28932..31226
                     /locus_tag="ACL297_000870"
     CDS             28932..31226
                     /locus_tag="ACL297_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGR0071155.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELCIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            31236..34352
                     /locus_tag="ACL297_000871"
     CDS             31236..34352
                     /locus_tag="ACL297_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 98 C-terminal
                     domain-containing protein"
                     /protein_id="HGR0071156.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            34409..36175
                     /locus_tag="ACL297_000872"
     CDS             34409..36175
                     /locus_tag="ACL297_000872"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGR0071157.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            36321..37472
                     /locus_tag="ACL297_000873"
     CDS             36321..37472
                     /locus_tag="ACL297_000873"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGR0071158.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            37617..38501
                     /locus_tag="ACL297_000874"
     CDS             37617..38501
                     /locus_tag="ACL297_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGR0071159.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            38900..39151
                     /locus_tag="ACL297_000875"
     CDS             38900..39151
                     /locus_tag="ACL297_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HGR0071160.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            39139..39429
                     /locus_tag="ACL297_000876"
     CDS             39139..39429
                     /locus_tag="ACL297_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGR0071161.1"
                     /translation="MGALGYYEGFVPYVSNQYQNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
BASE COUNT        12178 a         6804 c         8558 g        11899 t
ORIGIN      
        1 aggtgccctt gcagtcaaca caactgtaga tagctataga gtcaatgcca atggtgaatg
       61 ggtaaactaa acctaatata actcgttaat gctggcttcc tgtaagaact ctttaaagta
      121 ttccctacaa ataccatatc ctttcagtag ataatatacc cttgtaggaa gtttagatta
      181 aaaaataact ctgtaatctc tagccggatt tatagcgcta gagactacgg agtttttttg
      241 atgaggaaag aatggcggca ctcaagagac tctttaagag agttacgggt tttaaactat
      301 taagccttct ccaattgcaa gagagcttca atctctgcca gggtgctagc ttgcgaaatg
      361 gctccacgga gtttggcagc gccagatgtt ccacggaggt agtgaggagc gaggccacgg
      421 aattcacgaa ctgcgacgtt ttctcctttg aggttaatca atcgtttcaa gtgttcgtag
      481 gcgatcttca tcttgtcttc aaaggtcaaa tcaggtagga tttctcctgt ttcaaagtaa
      541 tggttgattt ggttgaagag gtaaggattt cccatggcag ctcggccaat catgactgcg
      601 tcagcaccaa cttcttcgat gcgttgcttg gcttcttgga cagtacggat atcaccgttg
      661 gcgatgaatg gaatcttggt tagagcttgg gcaaccttgt acagggtctc aaggtctgcg
      721 tggccagtat acatttgttc acgggtacgg ccatgcatgg cgagggcaga aacacctgca
      781 gcctcagcag cgagggcatt ttctactgcc agagatgggt ccgcccagcc ggtacgcatt
      841 ttgacagtaa gtgggatatc aaggacagac tggaccttgt tgatgataga gtagatcttg
      901 tcaggatcct tgagccacat agctccagct tcgttcttca cgattttgtt gacagggcag
      961 cccatgttga tatcgacgat atcggtcttg gtgttttctt ggatgaattc tgctgcgcgt
     1021 gctaggctgt cttcatcgct accaaaaagt tggatagaga cagggttttc gccctcatcg
     1081 atatgaagca tgtgcagggt tttttcgttg ttgtattgga ttcccttgtc agagaccatt
     1141 tccattacaa cgagtccagc tccgagctct tttgcgatgg tacgaaaggc tgagttggtc
     1201 acgccagcca taggcgctaa aacggtacga ttgggaatct caacattgcc aatcataaaa
     1261 ggtgtattaa gatttgtcac gaatgagttc ctccaggtcc ttttcatcaa agttgtaagt
     1321 agtttggcag aattgacaag tgatttctac cccgtggtct tcctctttca tttcctgtaa
     1381 gtctgagctt ggaaggctgg caagagcgtt cataaagcgt tcatggctac agtcacattg
     1441 gaaacggatt tcttcttcag aaagacgctt gtaggcttcg tccccgtaga tagccttgag
     1501 gagggcttcg atatggtcgt cgctttcgag aagagtagag atagctggca tttcttggat
     1561 gcgtttttca aagcgagcaa tctcttcttt cttggctcct ggcaagactt gaactaggaa
     1621 accacctgca accttgacct tgtcttcctc gtccaaaagg acattgaggc cgaccgctga
     1681 aggcgtttgt tggctttcag taaggtaaaa ggcaaggtct tcaccgattt ctccagagat
     1741 gaggggagtt atagagttgt aaggatttcc agcaccgtag tctgtgataa cgaggaattg
     1801 accatttcca acaaaaggtc cgactaggac ttcaccagtc gcagtctttt tgatgtcaac
     1861 accaggattt tgaacatagc ctttgacgtt ccccttggta tcagcgacgg tgataatagc
     1921 acctagagag ctagagccca acaccttaac tgtaagtttg gtatttcctt tttcattggc
     1981 tgcgagaatc tggctggcga taagagttcg accaagcgct acagttgagc tagcttgggt
     2041 ttgatgtttt tcttgagcag tgcggacggt ttccgtgcta tcaaggacaa aagcacgaaa
     2101 ggctccgctt tctgatatag ttttaataat tttatccata gctactattt tagcataaaa
     2161 atgcccaaag ggggagccgt gtgtttactg attttcagga taatggacca ggaaatcagc
     2221 atgaaaataa aaagagaaac agattatttt agcatttgta agatttatgc tatgcttaag
     2281 gtagaaaatg aaaggggtaa caaatgtatt taggagattt gatggagaaa gccgagtgtg
     2341 gtcaattttc aatcctttcc tttctattac aagagtctca gacgaccgtc aaggctgtaa
     2401 tggaagaaac aggattttca aaagcaaccc taaccaaata tgtcaccctg ctcaatgaca
     2461 aggctttgga tagtggctta gagctggcta ttcactcaga agatgaaaat ctgcgtctgt
     2521 ctatcggtgc agctaccaag gggagagata ttcggagctt gtttttggag agtgctgtta
     2581 aataccagat tttggtttat cttctctacc accaacagtt tttagcccat cagctggctc
     2641 aagaattggt gattagcgag gctacgcttg gtcgtcactt ggctggttta aatcagattt
     2701 tgtcagaatt tgatttatcc atccaaaatg gacgttggcg aggtccagag catcagattc
     2761 gctatttcta tttctgtctt ttccgaaagg tctggtcgag tcaggaatgg gaaggtcaca
     2821 tgcagaaacc agagagaaaa caggagattg ccaatttaga ggaaatctgc ggtgcaagtt
     2881 tgtctgtggg gcagaaattg gacttggttc tctgggctca catcagtcaa caacgtcttc
     2941 gggtcaatgc ttgtcagttt caagtcatag aagagaaaat gcgagggtat tttgacaata
     3001 ttttctacct tcgtttgctg agaaaggttc cgtccttttt tgctgggcaa catattccac
     3061 taggagttga ggatggtgag atgatgatat tcttctcttt tctcctatct catcgcattc
     3121 ttcctcttca tactatggag tatattcttg gttttggagg gcagttggca gatttactga
     3181 cgcaattgat tcaagaaatg aagaaggagg aactattggg ggattataca gaggaccatg
     3241 tcacctatga actcagtcag ctttgtgctc aagtctatct ctataagggc tatattttac
     3301 aggatcgcta caagtaccag ttagagaatc gtcatccata tttactgatg gaacatgatt
     3361 ttaaagagac agcagaggag atttttcatg ctctacctgc ttttcaacag gggacagatt
     3421 tagataagaa gattctctgg gaatggctcc agttaatcga atatatggct gaaaacggtg
     3481 gccagcatat gcggattggt ctggatttga catctggttt tcttgtcttt tcaaggatgg
     3541 cagccatttt gaaacggtat ttggaataca atcgttttat taccattgaa gcctatgacc
     3601 ctagtcggca ttatgatttg ctggttacca ataacccgat tcataagaag gaacagacac
     3661 cagtctatta tttaaaaaat gacttggata tggaggattt ggtagcgatt cgccagttat
     3721 tattcactta aaaggcttgg ttaatccagg tcttttttgt gaaattcaca caatctcctc
     3781 acattttttt aaaaattaaa aaaagttgat aaacaagaaa gcgctttatt ttgtatacta
     3841 gtaagtgtaa agaggaaaca cctcaagatc tttatcagga ggacagtaca tgtcacaaga
     3901 aaaatacatc atggccattg accagggaac tacaagttct cgtgccatca ttttcaacaa
     3961 aaaaggggaa aaggttagct cgagtcaaaa agagtttacc cagattttcc ctcaggcagg
     4021 ttgggttgag cacaatgcca atgaaatttg gaactctgtt cagtcagtta ttgcgggtgc
     4081 tttcatcgaa agtggtgtca agccaaatca aatcgaggca atcgggatta ccaaccaacg
     4141 tgaaacaacg gttgtctggg ataagaaaac aggacttcct atctacaatg ctatcgtttg
     4201 gcagtcacgc cagacagcac ctttggctga gcaactaaaa agccaaggtt atgtggaaaa
     4261 attccatgaa aagactggtt tgattattga tgcttacttc tctgctacca aggttcgttg
     4321 gattttggat catgtagaag gtgctcaaga gcgagcagaa aaaggggaat tgctctttgg
     4381 tactatcgat acttggttgg tttggaaatt gactgacggt gcggctcacg tgactgacta
     4441 ctcaaatgca gctcgtacca tgctttataa cattaaagaa ctcaaatggg atgatgagat
     4501 tttggaaatc cttaacattc cgaaggctat acttccagaa gttcgttcta actccgaaat
     4561 ctacggcaag acagctccat tccatttcta cggtggagag gtgccaatct caggtatggc
     4621 tggggaccaa caagcagccc tctttggaca gttggctttt gagccaggta tggttaagaa
     4681 tacttatgga acaggctctt tcatcatcat gaatactggg gaagagatgc agttgtctga
     4741 aaacaacctc ttgacaacca ttggttacgg aatcaacggt aaggtttatt atgccttgga
     4801 aggttctatc ttcatcgcag gaagtgctat tcagtggctt cgtgacggtc ttcgcatggt
     4861 tgaaaattca ccagaatctg aaaaatacgc tcgtgattct cacaacaacg atgaagttta
     4921 tgtcgttcca gcctttacag gtctaggcgc tccatactgg aaccaaaatg ctcgtggttc
     4981 cgtctttggt ttgactcgtg gaacaagcaa agaagacttt atcaaggcga ctttgcaatc
     5041 tattgcttat caagtgcgtg atatcatcga caccatgcaa gtggatactc agaccgccat
     5101 tcaagtactg aaggtggatg gtggtgcagc catgaacaac ttcctcatgc agttccaggc
     5161 ggatatttta ggcattgaca ttgcacgtgc taaaaacttg gaaacaacag ctctaggagc
     5221 ggccttccta gcaggtttgt cagtagggta ctggaaagac ttggacgagt tgaaactctt
     5281 gaacgagaca ggagaactct ttgagccatc tatgaacgaa tctcgcaagg aacaactcta
     5341 caagggctgg aagaaggctg tgaaagcaac tcaagtcttt gcggaagtag acgactaata
     5401 ctggcagaat aaagcgattt atttagaaag tgtgtaaata tggaattttc aaaaaaaaca
     5461 cgtgaattgt caattaaaaa aatgcaggaa cgcaccctgg acctcttgat tatcggtgga
     5521 ggaatcacag gagctggtgt agccttgcag gcggcagcta gcggtcttga gactggtttg
     5581 attgaaatgc aagactttgc agaaggaaca tctagtcgtt caacaaaatt ggttcacgga
     5641 ggtcttcgct acctcaaaca attcgacgta gaagtggtct cagatacggt ttctgaacgt
     5701 gcagtggttc aacaaatcgc tccacacatt ccaaaaccag atccaatgct cttaccagtt
     5761 tacgatgaag atggagcaac ctttagcctc ttccgtctta aagtagccat ggacttgtac
     5821 gacctcttgg caggtgttag caacacacca actgcgaaca aggttttgag caagaaagaa
     5881 gtcttggaac gccagccaaa cttgaagaag gaaggcttgg taggaggtgg agtgtatctt
     5941 gacttccgta acaacgatgc gcgtctcgtg attgaaaaca tcaaacgtgc caaccaagac
     6001 ggtgccctca ttgccaacca cgtgaaggca gaaggcttcc tctttgacga aagtggcaag
     6061 attacaggtg ttgtagctcg tgatctcttg acagatcaag tatttgaaat caaggcccgt
     6121 ctggttatta atacaacagg tccttggagt gataaagtac gtaatttgtc taataaggga
     6181 acgcaattct cacaaatgcg cccaactaag ggagttcact tggtagtgga ttcaagcaaa
     6241 atcaaggttt cacagccagt ttacttcgac acaggtttgg gtgacggtcg tatggtcttt
     6301 gttctcccac gtgaaaacaa gacttacttt ggtacaactg atacagacta cacaggtgat
     6361 ttggagcatc caaaagtaac tcaagaagat gtagattatc tacttggcat tgtcaacaac
     6421 cgctttccag aatccaacat caccattgat gatatcgaaa gcagctgggc aggtcttcgt
     6481 ccattgattg cagggaacag tgcctctgac tataatggtg gaaataacgg taccatcagt
     6541 gatgaaagct ttgacaactt gattgcgact gttgaatctt atctctccaa agaaaaaaca
     6601 cgtgaagatg ttgagtctac tgtcagcaag cttgaaagta gcacatctga gaaatatttg
     6661 gatccatctg cagtttctcg tgggtctagc ttggaccgtg atgacaatgg tcttttgact
     6721 cttgctggcg gtaaaatcac agactaccgt aagatggctg aaggagctat ggagcgcgtg
     6781 gttgacatcc tcaaagcaga atttgaccgt agctttaaat tgatcaattc taaaacttac
     6841 cctgtttcag gtggagaatt gaacccagca aatgtggatt cagaaatcga agcctttgcg
     6901 caacttggag tatcacgtgg tttggatagc aaggaagctc actatctggc aaatctttac
     6961 ggttcaaatg caccgaaggt ctttgcactt gctcacagct tggaacaagc gccaggactc
     7021 agcttggcag ataccttgtc ccttcactat gcaatgcgca acgagttggc tcttagccca
     7081 gttgacttcc ttcttcgtcg taccaaccat atgctcttta tgcgtgatag cttggatagc
     7141 atcgttgagc cagttttgga tgaaatggga cgattctatg actggacaga agaagaaaaa
     7201 gcaacttacc gtgctgatgt cgaagcagct ctcgctaaca acgatttagc agaattaaaa
     7261 aattaagaaa aaataaaaga ggtggagggc agcattcctt gtcgcccgtc ccttcttttt
     7321 aatggagaca gaaagatgat gaatgaatta tttggagaat ttctagggac tttaatcctg
     7381 attcttctag gaaatggtgt tgttgcaggt gtggttcttc ctaaaaccaa gagcaatagc
     7441 tcaggttgga ttgtgattac tatgggttgg gggattgcag ttgcggttgc agtctttgta
     7501 tctggcaagc tcagtccagc tcatttaaat ccagctgtga cagttggtgt agctttaaaa
     7561 ggtgacttgc cttgggcttc cgttttgcct tatatcttag cccagttcgc aggggccatg
     7621 ctgggtcaga ttttggtttg gttgcaattc aaacctcact atgaggcaga agaaaatgca
     7681 ggcaatatcc tggcaacctt cagtactgga ccagccatca aggatactgt atcaaacttg
     7741 attagcgaaa tccttggaac ctttgttttg gtgttgacaa tctttgcttt gggtctttac
     7801 gattttcagg caggtatcgg aacctttgca gtgggaactt tgattgtcgg tatcggtcta
     7861 tcactaggtg ggacaacagg ttatgccttg aacccagctc gtgaccttgg acctcgtatc
     7921 atgcacagca tcttgccaat tccaaacaag ggagacggag actggtctta cgcttggatt
     7981 cctgttgtag gccctgttat cggagcagcc ttggcagtgc ttgtattctc acttttctag
     8041 tttatactct tcgaaaatca aattcaaacc acgtcagcgt cgccttaccg tactcaagta
     8101 cagcttgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta gaaaacaatt
     8161 atgttgatag agcttgggca agagcccaat ttcagcaaaa aatgaagtaa atcttctcat
     8221 aataaaacgc atcatatcaa gcacgaaaat tccacgaggt caactacagt cagaaagctg
     8281 aacaacaagc caaaacgccc aaaaaaggcg gcaaaaagca agcacctgca agcaacgtgc
     8341 cgaaatggtc aaatcctgat tatgtcaacg aattagaccc aaaaatcgtt gatatgctag
     8401 tagaatttca caagtcacaa ggcactttgg aaactcccga ggcgcaagca gaaatcgccc
     8461 aaaaacgtga agaaatcgag caaaggagag ctgagcttga ggataaaaaa caagagcttt
     8521 tgaaccgctt gaacaaatag agtttcgcaa gtattatgct tacaaattac ttgagcaatt
     8581 aactaaaata taaaccctgc ctagatataa aggcagtgtt tatattttag aaattcacgt
     8641 aggttgttac ggtttttaca tacccagtat agtttgagtt tctatagtat tcagtgataa
     8701 acttccattt tctttgagca acatggatat aagtacttgt tatgtagtat ggatatgggc
     8761 tttgtgaatc caagtaagac tgataagctt gtataccaaa atatgctcca ccaattattg
     8821 caccccatgg accccccaat aaagcaccta tcctaccaat catataactg attccagcac
     8881 cagtcatgaa gttagcgaat gtgttagctt gtttattccc atgtattgtg ttgacgtaat
     8941 tccaaacatt aggatcgtat gatctaaaag atatatttag gtcgatttca ttcttttgat
     9001 aagccatata aaatgcccca ttgatataga cgccgtcagc acgtcgttca atagtgtcta
     9061 cacttccatc tggattgaca acctcaagaa cttcatcgct taaaatattt acttgcgtat
     9121 ctccgaaccg cactgatgag ccattctcaa actgagcctc accagataca actttagagt
     9181 ttgccgataa gctatcatca gcaaaaacaa caagcgacgg ggaaatgcta gacatacaga
     9241 aaacagacat aactagcaaa cacatgcatt taaacatctt agacataacg gaaactcttt
     9301 tgtatttttg atttttttca acttttatta tacaataaaa ccaaataaaa agaaagcggt
     9361 aacaatatgc ttaatgcgaa aattttttat atatttttat gtttgatcgt tatcgaaact
     9421 acaggcttgt tgttgttgaa aagaggtctc gaaatgggtt atttagacac agaagctatt
     9481 atcctcgcag ttttttcatt tgctttttac aacctatgtt cattcgcttg ggtctgctct
     9541 acaataaaaa ataaatagac gtattttcaa aaaaaaaaaa aatgaatatt tatattagca
     9601 aaatgacgat ttaaatcgtc gtttttttgt agtacgacgg gcatgtcgta tatctgaggt
     9661 gtaagtcctc ggtgggcacc tgctaccggt gaacccaata gcgattccca agcctgacta
     9721 tcgtgaggta gcggattaaa acggtctggg gatagaccgt tttaagtctg acgcaggaaa
     9781 taagaatcgt cagaggaagg gatagcgaaa tcgtggctct acgaacagga acgtgatagt
     9841 aaggcgtata tagcggataa ggaggcttca aactctaaag tccaaaaagg tagtcgtaac
     9901 ctatatgtgt aaatcacgag agtaattgaa ttcggactaa ggtttgtgtg aaaaagataa
     9961 atctttctag agtctaaaga ctctgcgtca gatttcctat tttcactgta accttttaac
    10021 gtcctcatat cttgtataaa cgaggaaaga tgtacgactt atcccgtgag gtttcatgag
    10081 cgctgaaagc gtagtaacaa cgaatcatga gaagtcagcc gagcccatag tagtgaggaa
    10141 acttctgtag tggaagtgga gcgaaggggt gaatactcaa acagtctggg gagagactgt
    10201 ttgaggtctg tcgctagaaa gagaaaacga cagatcgaag taatcctact tcacttgtgt
    10261 ctgtaaaatg agtggtctga tagaactgga ctttgacacg taatgactag atgaaggttc
    10321 accaatataa gatgtccctc aggcaccaaa acaagaaagg aatacgcaca tgtcaaaact
    10381 gctagataag atattatcac gcgaaaatat gctggaagcc tacaatcaag taaaatccaa
    10441 taaaggctca gctgggattg atggaatgac tatcgaagag atggataatt atctcagaca
    10501 aaactggcgc ttgactaagg aactgataaa acagagaaaa tataagcctc aaccagttct
    10561 taaagttgag atacctaaac cagacggagg catccgtcaa ctaggaattt caacagttat
    10621 ggatagaatg attcaacagg ccattgtcca agtcatgagc cccatttgtg aaccccattt
    10681 ctcagatacg agttatggtt tcagaccaaa taggtcatgt gaaaaagcca tcatgaagct
    10741 cttagaatac ttaaatgacg gctatgagtg gatagtggat atagacctag agaaattttt
    10801 cgatacagtt cctcaagata gattgatgtc cttagtacat aacattatcg aagacggaga
    10861 tacggaatcc ttgattcgta agtatcttca ttcaggtgtt atcattaatg gtcaacgtta
    10921 taaaacacta gttggtacac cacagggagg aaatttatct cctctcttat ccaatatcat
    10981 gcttaatgaa ttggacaagg aattagaaaa gaggggactt cgatttgtgc gctacgcaga
    11041 tgattgtgtg attacggtcg taagcgaggc agccgctaag cgtatgatgt attcagtcag
    11101 tcgttttatt gaaaaacggc taggtttgaa agcaaacatg accaagacta agattaccag
    11161 accaagagag ttgaaatatc taggttttgg gttctggaaa tcatcagatg gttggaaaag
    11221 tcgtccacat caagatagtg ttcggagatt taagcttaaa ttgaagaaac taacacagag
    11281 gaaatggagt atagacctaa caagacgtat tgagcaactg aatttgtcta ttcgaggatg
    11341 gataaactat ttctcattgg gaaatatgaa aagtatagtc gccagcatag atgagcgctt
    11401 gcgtactcgc ctacgagtga ttatctggaa gcaatggaag aagaaatcga gacgattatg
    11461 gggattgctt aagttagggg ttcctaaatg gatagcagat aaggtatctg gctggggcga
    11521 ccattatcaa ttagtagctc agaagtcggt acttaaacgt gctatatcaa aaccagtcct
    11581 ggaaaaacgt ggactggttt cgtgtttgga ttattacctt gaacgacatg cgttaaaagt
    11641 tagttgaacc gctgtatgcc gaacggcacg tacggtggtg tgagaggggc tagagattat
    11701 cccctactcg attaactccc ctgaaattta ttttaattat gcaaatttca cgtatttttg
    11761 atgctgagac gacgatcctg ggaacttttc agatattttt ttgactatct aaatctatca
    11821 ttagaaaagc ttagagcgcc aaaggatttg agcgtttttc tgatttttaa gactttttcc
    11881 agtctctttt tcgattgaag atgtaattat tctactaact aactaacttc ttagtactag
    11941 ccaacaacga taatcataat tcctcctaaa attaggaata ataaaggcaa tagtttttgt
    12001 tttttcatgt aaaaaacctc acttttgttt tctgctattt tatgctaaaa tattaaaaat
    12061 caaatttaat tccaaagttt gtaactaaag ggggagcgct acatgtctaa ttcatttgtc
    12121 aagttgttag tctctcaatt atttgcaaat ttagcagata ttttctttag agtaacaatc
    12181 attgctaaca tatacattat ttcaaaatca gtaattgcca catcactagt tcctatctta
    12241 ataggaatat cctcttttgt tgcgagtctt ttagttccgt tggttactaa aaggttagcg
    12301 ctaaataggg ttttatcttt atctcaattt ggaaagacta tattattggc gatactggta
    12361 ggaatgttta ccgtaatgca atccgtagcg cctttggtga cctatctatt tgttgttgca
    12421 atttccatac tagatggttt tgcagcacct gtttcctatg ctattgtgcc acgctatgcg
    12481 accgatttgg gtaaggctaa ttcagcctta tcaatgactg gtgaagctgt tcaattgata
    12541 ggttggggat taggtggact cttgtttgca acaattggtc tgttacctac cacgtttatc
    12601 attttagtct tgtatatcat ttctagcttt ctgatgttat ttcttcctaa cgctgaagtg
    12661 gaggtgttag agtcagaaac taatcttgaa attttgctca aaggttggaa gttagttgct
    12721 agaaatccta gattaagact ttttgtatca gcaaatttat tggaaatttt ttcaaatacg
    12781 atttgggttt cttccattat acttgttttt gtaacggagt tattaaataa aacggaaagt
    12841 tactggggat attctaatac agcatactct attggtatta taattagtgg cttaattgct
    12901 tttaggctat ctgaaaagtt ccttgctgct aaatgggaaa gtattctttt tcctcttgta
    12961 gctatggcaa tagtgacact gactattcta tattttccaa acgcacagat gtttttatta
    13021 ttttcagctt tagttggtat gttatcgcaa ctaaaagaag ttcctgaaag tgtatttctt
    13081 caggaaacag tagaggaaaa tcatttagtt aatgtctatt ccgttcttga agtgatttct
    13141 acattagcat tttcagtatt tgttttgcta atgagctata ttactgagag ttttggtatt
    13201 agcatcagtt tttggctatc agccatttgt ctgatgatag aagctatttt aatttatatc
    13261 agacgagatt attttaaatg agagagtggt taaaaagtct ttaaaaatca gaaaagcgca
    13321 tagtatcagg tgttgaaaaa acttgatatt gtgtgtttta ttatgggaag atttacttca
    13381 ttgtctcctg aaatggagtt ttgaagcagg atcactctat ttgaaaatat acatagaaaa
    13441 actgtataat ccttaaaata agctgaaatc tattggaaac atacggtaaa caattgaaaa
    13501 gatactatat aaagtggtac aatggtagaa aaataagcta gtaagaatca ctcttatttt
    13561 agcgaaaaat tacagaaatg aatggttttt cttgatgaaa cagagaaaag aattgtacct
    13621 ctttcttggt cggacagcct tgtattttct tatctttcta gggctgcttt acttctttag
    13681 ctatcttggt cagggtcaag gaagctttat ctataatgaa ttttaacttg aaggagaatc
    13741 cctattgtgt caaataaacc aatagcagat atgattgaaa ccattgagca ttttgctcag
    13801 acacagccta gctatcctgt ctataatgtt ttggggcagg aacacactta tggcgattta
    13861 aaggctgatt cggatagttt ggctgcagtc attgaccaac taggcttgcc tgagaagtct
    13921 cctgtggttg tttttggtgg ccaagaatat gaaatgttgg caacctttgt agcgctgact
    13981 aagtcaggtc atgcctacat tccaattgat agccattcgg ccttggagcg agtttcagct
    14041 attttagaag tagcagagcc aagcttgatt attgccattt cagcctttcc cttggagcag
    14101 gtttctacac caatgataaa tctagctcag gttcaagaag cctttgccca agggaataac
    14161 tatgaaatca cgcatccagt caagggagat gataattact acattatctt tacttctggt
    14221 acgactggta agcctaaggg agtgcagatt tcacatgata atctcctcag ctttacaaac
    14281 tggatgatta cggataagga atttgcgaca ccgagtcgtc cgcaaatgct ggcacagcca
    14341 ccttattctt ttgacttgtc tgtcatgtat tgggcaccga ccttggcact tggtggtacg
    14401 cttttcactc ttccttcagt catcactcag gactttaagc aactctttgc ggctatcttt
    14461 tcattgccaa tcgctatctg gacatcaaca ccatcctttg cagatatggc catgttgtct
    14521 gaatacttca acagtgagaa aatgcctgga atcactcatt tctactttga tggtgaagaa
    14581 ttgacggtca aaacagctca aaaactgcgc gagcgtttcc caaatgccca tatcatcaat
    14641 gcttacggcc caacagaagc gacagtagct ctgtcagcag ttgccgtgac agacgagatg
    14701 ttagcgactc tcaaacgcct accaatcggc tataccaagg ctgattctcc aacctttatc
    14761 attgacgagg aaggaaataa actgccaaat ggtgagcagg gagaaatcat tgtttctggg
    14821 ccagctgttt caaaaggtta tatgaacaat cctgaaaaaa cagcagaagc cttctttgag
    14881 tttgaaaatc tgccagccta tcacacaggc gatgtgggaa ccatgacaga tgagggcttg
    14941 cttctctacg gcggacgcat ggacttccag attaagttca acggttaccg cattgagtta
    15001 gaagatgtct ctcaaaacct caacaagtct cgctttatcg aatctgctgt cgcagtaccg
    15061 cgctataaca aggaccacaa ggtacaaaat ctattggctt atgtcatctt aaaagacggt
    15121 gttcgtgagc agtttgagcg agatatcgat attaccaagg ccatcaagga agacctgaca
    15181 gacatcatga tgtcctacat gatgccatct aaattccttt accgagacag tttgccacta
    15241 actccaaatg gaaagattga catcaaagga ttgattaacg aggtgaataa gagatgatgg
    15301 agttttttca acagcttcct catttagagc catatggcaa tcctcagtat tttgtttatg
    15361 tgattgctgc aaccttgccc atctttatag gcctcttttt caagaaacgc tttgcctggt
    15421 atgaagtgtt ggtaagtctc ttctttattg tcaccatgtt ggtgggtgga aagaccaatc
    15481 aactagctgc cttgggtatt tacctttgct gggaaatatt gctcctgctt ttctacaagc
    15541 attatcgaaa aagcaaggat ggcaagtggg tcttctactt agttagtttt ctgtccctac
    15601 ttccgattat ctttgtcaag gtgcaaccag ctatcaatgg aacgcagtct ttgcttgggt
    15661 tcttgggaat ttcttacctg acctttcgtt cggttgggat tatcatcgag ctgagagatg
    15721 gagtgattaa ggattttacc ctctgggaat tcctccgttt ccttctcttc atgccaactt
    15781 tctcgagtgg tccaatcgat cgctttaagc gatttaatga aaattatcag accattcctg
    15841 agcgagatga gttgatggat atgctggatg aatctgtccg ctatatcatg cggggctttt
    15901 tgtataagtt tatcctagct catgttttag gagagacctt actacctcct ctgaagaatt
    15961 tagccttgca gtcaggtggc ttctttaatc tctatgcctt ggcagttatg tatacttttg
    16021 gtctggagct cttctttgac tttgcaggtt attctatgtt tgctttggcc atctcaaact
    16081 tgatgggaat ccgtagccct atcaacttta acaagccctt tttatcaagg gatttaaagg
    16141 agttttggaa tcgctggcat atgagtctgt ccttctggtt ccgtgacttt gtctttatgc
    16201 gaatggtgat ggtgttaacc agaaagaaag tctttaaaaa tcgtaatgta acctcaagca
    16261 tggcctacat tgtaaatatg ctgattatgg gattttggca tggcgtgacc tggtactata
    16321 tcgcctatgg actctttcat ggactaggtt tggtcatcaa tgatgcctgg gttcgcaaga
    16381 aaaaaacgct caataaggaa cggaaaaaag cagggaaggc tgccctacct gagaatcgct
    16441 ggattcagtt gcttggcatg gttgtcactt tccatgttgt catgttgtca ttcttaatct
    16501 tttctggatt cttgaataat ctatggttta aaaaataaaa ggaaataaaa tatggatatc
    16561 aaatcagaag ttatcgaaat tattgatgag ttgtttatgg aagatgtttc tgacatgatg
    16621 gatgaagatc tttttgatgc aggtgtcttg gatagtatgg gaacggttga gttgattgtg
    16681 gagattgaga accgttttga cattcgtgtc cctgtaacag agtttggtcg tgacgactgg
    16741 aatacagcta ataaaattat agctggtatt gtggagctac aaaatgctta aacgcttatg
    16801 gatgatcttc ggaccggtct tgatcgctgg tttgttggtt tttctgctca ttttctttta
    16861 tcctactgag atgcatcata atctaggagc tgaaaagcgt tcagcagtgg ctactactat
    16921 cgatagtttt aaggagcgaa gtcaaaaagt cagagcacta tctgatccaa atgtgcgttt
    16981 tgttcccttc tttggctcta gtgaatggct tcgttttgac ggtgctcatc ctgcggtatt
    17041 agctgagaaa tacaatcgtt cctaccgtcc ttatctttta ggacaggggg gagctgcatc
    17101 gcttaaccaa tattttggaa tgcaacagat gttacaacag ctggagaata aacaagttgt
    17161 gtatgttatc tcacctcagt ggttcagtaa aaatggctat gatccagcag ccttccagca
    17221 gtattttaat ggagaccagt tgactagttt tctgaaacat caatctgggg atcaggctag
    17281 tcaatatgca gcgactcgct tactgcaaca gttcccaaac gtagctatga aggacctggt
    17341 tcagaagttg gcaagtaaag aagaattgtc gacagcagac aatgaaatga ttgaattatt
    17401 ggctcgtttt aatgaacgcc aggcctcctt ttttggtcag ttttcggtta gaggctatgt
    17461 taactacgat aagcatgtag ctaagtattt aaaaatcttg ccagaccagt tttcttatca
    17521 agcaatagaa gatgttgtca aagcagatgc tgaaaaaaat acttccaata atgagatggg
    17581 aatggaaaat tatttctata atgagcagat caagaaggat ttgaagaaat taaaggattc
    17641 tcagaaaagc tttacctatc tcaagtcgcc agagtataat gacttgcagt tggttttaac
    17701 acagttttct aaatctaagg taaacccgat ttttatcatt ccacctgtta ataaaaaatg
    17761 gatggactat gctggtctac gagaggatat gtaccaacaa acggtgcaga agattcgcta
    17821 ccagttagaa agtcaaggtt ttaccaatat agcagatttt tctaaggacg gcggggagcc
    17881 tttctttatg aaggacacca ttcaccttgg ttggttgggt tggttggctt ttgacaaggc
    17941 agttgatcct ttcctatcca atcccacacc agctccgact taccatctga atgagcgctt
    18001 tttcagcaaa gattgggcga cttatgatgg agatgtcaaa gaatttcaat agataatata
    18061 atatagaaga gttcaagaat gaaactagtt ttcacgtttt ttcttgactc tttttttgct
    18121 tgtgatataa ttaactggta aacaaaatgt caaagaatag tatttttctc ttaaaaaaga
    18181 gaagtctaaa aggaaaggag tcagatatga gacagctagc aaaggatatc aatgcttttt
    18241 tgaatgaggt gattttgcag gcggaaaatc agcatgaaat cctaataggt cattgcacta
    18301 gcgaggtggc cctgaccaat actcaggagc atatccttat gctcttgtca gaggaatctt
    18361 taacaaattc agaattggct cgtcgtctca atgtcagtca ggcggcagtt accaaggcca
    18421 ttaagtcttt ggtcaaggaa gggatgttgg aaacatctaa agattctaaa gatgcgcgtg
    18481 tgatttttta tcagttgact gacttggctc gtccaatcgc tgaggagcac caccatcacc
    18541 atgagcatac acttttaacc tatgaacaag tggcgactca gtttactcca aatgaacaaa
    18601 aagtgattca gcggtttttg actgctttag taggagaaat caaataatga gatatattac
    18661 ggtagaggat ttgtccttct attatgataa ggagcctgtt cttgaacata tcaattattg
    18721 tgttgatagt ggggaatttg ttaccttgac tggggaaaat ggagcggcta agacgacgct
    18781 catcaaggct agtcttggaa ttctgcaacc acgcattgga aaggtggcta tttcaaagac
    18841 aaatacgcaa ggtaagaaat tgagaatagc ctatcttcct caacaaattg ccagttttaa
    18901 tgctggtttt ccaagtacgg tctatgaatt tgtcaagtcg ggtcgctatc cgagaaaagg
    18961 ctggttccgt cgtttgaatg ctcatgatga ggagcatatc aaggctagtc tggactcagt
    19021 tggcatgtgg gaacatcgag acaaacgctt ggggtctcta tctgggggac aaaagcagcg
    19081 agcggtaatt gcgcgtatgt ttgcttctga ccctgatgtg tttatcctag acgagccgac
    19141 aacggggatg gatgcaggaa gtaaaaatga attttacgaa ctcatgcacc acagcgccca
    19201 tcatcatggc aaggctgttt tgatgattac ccatgaccct gaagaagtta aggattatgc
    19261 ggatcgcaat attcatctag tccgtaacca agactcgcca tggcgttgtt tcaatgttca
    19321 tgagaatggc caggaggtgg gccatgctta gtttattatc ttacgacttt atacagcgcg
    19381 cctttttggc ggttattgct atgagtcttt tctcaccggt attgggaacc ttcctcatct
    19441 tgcgtcgtca gagtttgatg agtgataccc ttagccacgt ctcactttca ggtgtagcct
    19501 ttggtctggt tttggggatt tctccaactg tttctactat tgccattgtc ttgattgcgg
    19561 cggtctttct ggagtatctc cgtacggttt acaagagctt tatggaaatc gggacagcta
    19621 tcctcatgtc aacaggtctg gctgtttctc tgattgtcat gagcaagggt aaaagctcga
    19681 gttcaatgag tttggaccaa tatctctttg gttcgatcgt gactatcagt gaagaacagg
    19741 tcatttccct ctttgtcatt gcggtggttg ttttgatttt gacctttctc tttcttcgtc
    19801 ctatgtatat cttaactttt gacgaagata cggcctttgt ggatggcttg ccagttcgta
    19861 ccatgtccat tctttttaac atggtgacag gggtggctat tgcccttatg attcctgcag
    19921 caggagctct tctggtatcg accattatgg tcttgccagc tagtattgcc ctgcgtctgg
    19981 ggaaaaactt taaatcggtt atgctgcttg ccagtgcgat tggctttttg ggaatggtag
    20041 caggacttta catttcctac tatgcagaaa cacctgcaag tgcaagtatt accattattt
    20101 ttgtaactgt ctttatacta atcagtttag taagacgttt tatcaaatag gagacgaacg
    20161 tgaaaaaaat tagcttatta ctagccagtc tatgtgcctt gtttttagtg gcttgttcca
    20221 atcaaaaaca ggcagatggt aaactcaata tcgtgacaac cttttaccct gtctatgaat
    20281 ttaccaagca agtcgcagga gatacggcta atgtagaact cctaatcggt gctgggacag
    20341 aacctcatga atacgaacca tctgccaagg cagttgccaa aatccaagat gcagatacct
    20401 tcgtttatga aaatgaaaac atggaaacat gggtacctaa attgctagat accttggata
    20461 agaaaaaagt gaaaaccatc aaggcgacag gcgatatgtt gctcttgcca ggtggcgagg
    20521 aagaagaggg agaccatgac catggagaag aaggtcatca ccatgagttt gacccccatg
    20581 tttggttatc accagttcgt gccattaaac tagtagagca catccgcgac agcttgtcag
    20641 cagattatcc tgataaaaaa gagacctttg agaagaatgc agctgcctat atcgaaaaat
    20701 tgcaagcctt ggataaggct tacacagaag gcttgtctca agcaaaacaa aagagctttg
    20761 tgactcaaca cgcagccttt aactatcttg ccttggacta tggactcaaa caagtcgcaa
    20821 tctcaggcct ttctccagat gcagagccat cagctgctcg cttggcagaa ttgacagagt
    20881 atgtcaagaa aaataaaatc gcctatatct attttgaaga aaatgcctca caagcccttg
    20941 ctaacacact ttcaaaagaa gcaggtgtca aaactgatgt cctcaatcct ttagaaagtc
    21001 tgacagaaga ggacaccaag gctggagaaa actacatttc cgtgatggag aaaaacctca
    21061 aggctttgaa acaaacaaca gaccaagaag gcccagcaat cgaacctgaa aaggcagagg
    21121 ataccaagac agtccaaaat ggttacttcg aggatgcagc tgtcaaggac cgcaccttga
    21181 gtgactatgc aggtaactgg caatcagttt atcctttcct tgaagacggc acgtttgacc
    21241 aagtctttga ctacaaggct aagttgactg gtaagatgac ccaggctgag tacaaggctt
    21301 actatacaaa aggctatcag acagatgtga ctaagattaa cattactgat aatactatgg
    21361 aatttgttca aggtggacaa agcaagaaat acacttacaa gtatgtcggt aagaaaattt
    21421 tgacttacaa gaaaggcaat cgtggcgtgc gtttcctctt tgaagccaca gatgctgacg
    21481 ctggacaatt caaatatgtt cagtttagtg accacaatgt tgccccagtt aaggcagaac
    21541 atttccatat cttttttgga ggtacaagcc aagaagctct atttgaagaa atggataatt
    21601 ggccaaccta ctacccagat aacctatctg gccaagaaat cgcccaagaa atgttggcgc
    21661 attgatgaga aatcgactag ttcataagag ctagtcggtt ttttgatact agatggctaa
    21721 cttcattata gacctttcag aactaaggga acctgaaaaa ataaataaaa ctcttgaaat
    21781 atctggactt atatgctaga atgtagttag tgtataaggt tcattagaac accaaagccc
    21841 ctaactagtg ccatagttaa gggctttttt gacgtatctt agcgatttaa tgggtgttta
    21901 taggctagtc agtcggtcta cttcttacca tctagccatt tgcagacgaa atacaagata
    21961 attccagcta taacatctgc tataatagta agagcaagct ctggtataag gctcattagt
    22021 ttcacctcct ctcacgaacc cataggaacg taatcggtaa ccgatgacaa aagtattata
    22081 tcacactaaa tttagatcat caacctcact taatgcttga actattgtag attatatgct
    22141 aaaatgtagt taattgataa ggttcattag aacaccaaag cccctaacta tggccgtagt
    22201 taagggcttt tttgacgtat cttagcgatt taacgggtgt ttataggcta gtcactcggt
    22261 ctacttctta ctatctagcc atctgcagac gaaatacaag ataattccag ctataacatc
    22321 cgctataata gtaagagcga gctctatgat aaggctcatt agtttcacct cctctcacga
    22381 acccatagga acgtaatcgg taaccgatga caaaaatagt ataccacaat acatttagat
    22441 catcaaggtc acttaattct tgaaatatag ggtttaggcg acaaagtctt taaaatcaga
    22501 aaaacgcata atatcaggtg tgcaaaaact tgatactatg cgttttattg tgggaaggtt
    22561 tactccattt tctcctgaaa ttgagttttt gtccagcctc tgtttttagg gttgctgaga
    22621 aaataatgtc atgtggtgaa tatttgtaaa tcagtcagca gacagaacga atactcttcg
    22681 aaaatctctt cacatcatgt cagcttcgtc tttccgtata tatgtgactg acttcatcag
    22741 ttctatctac aacctcaaaa cagtgtttcg agctgacttt atcagttttc aaatctgtac
    22801 tttgagcaag ctgagactag cttcctattt gattttcatt gaatatcaga aacccattct
    22861 ccatcaaata attcgactgc gtctaataat ttttgatctg gcacggtgtc tgaaataaag
    22921 gttgtgtatt tggagagggg attaatttta aaaaatccag tcttgtaaaa tttagaacta
    22981 tcaatcagta agatggtttc atgggctttg tcaataatat tcttttttga aatagcttgg
    23041 ctgagagaag cttcataaac atattggtca tcaatacctc ttgctgaaca aaatgctaaa
    23101 tcgatattaa aatgatctaa taaagaattt tccttatcat agttgaccac ggaacaggat
    23161 tgatgtttga cctcgccaga tgtgataaag attttggagc tatctttaac agtttcagat
    23221 agggtttgtg cagtatgtaa accatttgta aaaataatca aattatcaag ttcagaaaga
    23281 tagggacaaa gttcgtagac agtagtactg gaatccagat aaacacacat accagaccga
    23341 ataaagtctt tagcgagact agcgattagt cttttttgcc tagtactttc tccttcccgt
    23401 atttgatgag aaagttcaat tgtgttcata gaggacaggg tcacgtatcc gtgctttctt
    23461 ttgataagac cttgattttc taagaaaatt aaatcacgac gtaaggtact tgtgctggag
    23521 aaagtgattt ctgccagctc ttttacggca attctttttt tctttttaat aatttcaatc
    23581 aattcaagta cacgttcatc ttttatcata agctcctcct aatttatcat ttcaactata
    23641 ttatagcaca aattggagga atttgaatta tttttatgaa tattgggtta acatttgaac
    23701 attattcaag taagcgttca catattgaaa aaataaaatg tggggattat aataaagtta
    23761 atcaaggacg aagagagaag aaaaatggaa gcggttttag caatagattt aggtgcgact
    23821 tctggaagag caatcgttgg ttacctttct gaaaataaac tagtaatgga agaaataaat
    23881 cgcttttcta atctacctat tagagtaaaa gggcatttat cttgggatat tgactttcta
    23941 ctagctaaaa ttcttgaaag tatccgcttg gctaatacta gttacaagat tttatctatc
    24001 ggtattgaca catggggagt tgattttgga ctgattgata atgaaggtaa gctgttatca
    24061 caacctgttc attatcgtga tgaaagaaca aagggagtgt taaaggaaat atctgaaatg
    24121 actgaattag aaaaactgta ttcagagaca ggaaatcaga ttatggagat aaataccttg
    24181 tttcaactct ttaaggcacg tcaagaatct cctgactctt tctataagac caataagatt
    24241 cttttaatgc cagatttgtt taattatctc ttgacaggta agtttgctac agaaaaaagc
    24301 attgcttcaa caactcaatt atttgatcct aggagtcaaa attggaatca gaatatctta
    24361 aaactatttg aattggattc atctttactt cctgaaattg tttcagaggg aaatgttctt
    24421 ggaaggataa aaaaggagta tggtttaggc gatattcctg ttgtgaatgt ttgtagtcat
    24481 gatacagcaa gtgcgattgt ctcagtacct aagacagaag gtagtttatt tatttcatca
    24541 ggtacttggt ctttggttgg agtggaactt acttcaccga ttcttactac cgaatccttc
    24601 agttatggat ttacaaatga agtcggtaaa gatggagaga ttacatttct gaagaattgt
    24661 acagggttgt ggatcataga ggaactaaga cgttcatttg aacgaagagg gaaagcctat
    24721 tcttttgatg atattaggac aatggtggag aaagaaaaag aaaatcttcc tctgattgat
    24781 actgaatcaa ctgaatttgc aacagaatct gatatgcaca agactttgac agaatatcta
    24841 gcttatcatc atgaaactag agagtggaca gatggacaac tatttaagat tgtttatgaa
    24901 agcctagctg aaacgtatag gaaagcgata gagttactag aagaactaac tcataaggtt
    24961 tataagagga tatatgtgat tggaggaggt gctagagcca gttactttaa ccaaatgatt
    25021 gctgatagaa ctggtaaaga ggttcttaca ggttcgactg agggtacagc tgtggggaat
    25081 attgttgtgc agctcatagc tatgggacaa ttaaaaggga tggaagaagc tcaccatgtt
    25141 attgaggagt ttctacaatt agagagttat tactcccaaa agaattaaaa agattgagag
    25201 tttgtaaatt tgcctccctc ccccttctta agttttgtac aagaaggggg gataattggt
    25261 gaattgaaaa atatttagtg ttttgatatg aggaggacaa gaatgtcaga tgtaaaacaa
    25321 gaattaatta agtatggcaa gaagctagta gaaacagatt tgacgaaagg aacaggtggg
    25381 aatctcagtg ttttcgatcg tgaaaaacaa ttgatggcaa ttaccccgtc gggtattgat
    25441 ttctttgaaa tcaaagaatc cgatattgta gtgatggata ttaatggaaa tgttgtagag
    25501 ggagaacgct tgccatctag cgaatggtat atgcatttga ttcaatatca aactcgtgat
    25561 gatatcgatg caattatcca tgctcataca acttatgcaa cagtattagc ttgtctcaga
    25621 gaaccacttc cggctagtca ttatatgatt gcagtggcag ggaaagatgt tcgggtagct
    25681 gagtatgcaa catatggtac gaaagaattg gctgtgaatg cagctaaagc aatggaaggt
    25741 cgtagagcag ttttactagc gaatcatgga attttagcag gtgcacaaaa tttattgaat
    25801 gcatttaata ttgttgaaga agttgaatat tgtgcaaaaa tttattgttt agctaagaat
    25861 tttggagagc cagtagttct tcctgatgag gagatggatt taatggcaga aaaatttaaa
    25921 acatacggtc agagaaaata gggaggatat taatgttaaa acatataccg aaaaatattt
    25981 ctccagattt attgaagact ttaatggaaa tgggacatgg agatgaaata gtattagctg
    26041 acgcgaatta tccttctgcc tcatgtgcaa ataagctaat tcgttgtgat ggcgtaaata
    26101 ttccagaatt attagattcc attctgtatt taatgccatt agatagttac gtcgatagtt
    26161 caattcagtt tatgaacgtt gtttcgggtg atgatattcc taagatatgg ggtacctata
    26221 gacagatgat tgaaggtcat ggtacagatc ttaaaacgat tacttatctt agaagagaag
    26281 acttttatga acgtagtaag aaagcttatg ctattgttgc tacaggagaa acttcacttt
    26341 atgctaatat tatccttaag aaaggagtag ttgttgaaag agaaaatgtt taatagagga
    26401 attttagttg ccagtcatgg taattttgct agcggagctc tcatgaccgc agaaatgttt
    26461 gttggtgaga caacaaatga tagagttagg acattaggtt tgatgcctgg agagaatatt
    26521 gtagagtttg aacattattt taaaaatcaa gtaaatgaac tgttagactc aaatcaagag
    26581 gttatcgttt tgactgactt gattggagga agtcctaata atgtggcttt gtcacggttt
    26641 ttaaatttgg attcagttga tattgtaaca gggtttaata tccctctcct agtggaatta
    26701 atatcaagtt atgattcaaa aatcaattta gaagaaattg ttcaaaatgc tcaaaatagt
    26761 ttgtttaatg ttaaacaaca acttaacgta gaggaggaag aggatttatg tctatagagt
    26821 ttgttcgtat tgatgaccgt ctggtacatg gtcaagttgt cactacgtgg ctaaaaaagt
    26881 atgatattga gcaagttatc attgttaatg atcgcatctc agaagataaa acacgacaat
    26941 ctattttaaa gatttctgca ccggtaggtt taaaaattgt tttctttagt gtaaaacggt
    27001 ttgtggaagt tttaaactct gtgccaataa aaaagagaac aatgctgata tatacaaatc
    27061 caaaagatgt gtatgattct attgaaggaa atttaaaatt ggagtacctc aatgtaggac
    27121 agatgagtaa aacggaggaa aatgaaaagg taacgggagg tgtagctcta ggtgaagaag
    27181 acaaatatta ttttaagaaa atagttgata agggaacgag agttgaaatt caaatggttc
    27241 ctaatgataa agttacaatg ttggaaaaat ttttataaaa ataatttaag gaggtacagt
    27301 atatgctatt cacacaagca ttactggtga cattagttgg gattattgcc actattgact
    27361 ataatggacc gttatttatg attcaccgtc cgttagttac aagtgcaatg gttggcttag
    27421 tcttaggaga tttcacccaa ggtgttctta ttggttcagc tcttgaatta acttggctcg
    27481 gtgtaacagg tattggaggt tacactccac cagatactat ttcaggtgcg attattggta
    27541 ctgcatttgg tattttgtct ggtcaaggag aaactgctgg tatcgctata gcagttccaa
    27601 ttgcagttgc tactcaacag ttggatgttc ttgcaaaaac tttagatgtt tattttgtga
    27661 aaaaagctga taatgatgct aaaaacggag attattcaaa gatcggtttt tatcattatt
    27721 caagtttggt tttaatcact ttatttaaaa ttgtaccaat tttcctagct attatgcttg
    27781 gaggggaata tgtggcagac ttgtttgcta aggttccacc aatcgttatg cagggactta
    27841 actctgcagg tgctttacta ccttcaattg gttttggtat gcttttaaat atgatgctca
    27901 agaaaaatat gtgggtattc ttgttgattg gattcatttg ttctgtgtat ggaggaatgt
    27961 caaccattgg gatctcacta gttggtattg cggtagcata cttctacgat atgattggaa
    28021 gtaaaccaca agaaacaact tcaagtagtg atgttgagga ggatcttgat ctatgatgaa
    28081 taataaagta actaaagttg aacttaaaaa agttttcaaa cgaagtttta tgtatggttc
    28141 ttcatggaac tatgagagaa tgcagaacct aggttttcta tatacaattc ttccagtatt
    28201 gaaaaaacta tacccagaca aagattcagc ttctcctgca atgaaacgtc accttgagtt
    28261 tttcaatact catcaaacag cggcaccatt tattcttgga gttacttccg ctatggaaga
    28321 acaagaagga aatgaaggtg cagcttcaat tactggtatt aaagttggct tgatggggcc
    28381 actggctggt ctaggagata gtttgttctg gctgacacta gttcctatct gttttagtat
    28441 tggtgcgtct tattctaaag acggcggtgc tttaggtatc tttatcgcct taatattgtt
    28501 taatattatt aatattcctg ttaaatattt cggtttgaaa tatgggtata ctaagggttc
    28561 tagtcttatc caagaaaata atacaaaagg aacattgaat cgcgttacga gtatggcgac
    28621 agcattaggg ctagtactag tgggtggttt gattccatca atggttggta ttaattttgg
    28681 attagaattt aagcaggggg aacttgttat ttctgttcaa gaaatgatta caaaattaat
    28741 tccaggattt atccctatgg ctttgacttt attaatgtgt aaattaatta gaaaaggaaa
    28801 gaatccggtt gtactaatct ttagtgttat ggctattgga gttatcctag ttgttttagg
    28861 aattttgaag tagtagaaag tgtggaggtg gtatttggga tatcacctcc attttggaag
    28921 agaggtaaag agtgaaatta tggtataaga aagctgccgc aaattggaat gaagccttgc
    28981 cgattgggaa cggtcattta ggtggtatga tttatggttc agctacaaaa gaatgtattc
    29041 aactaaacga tgagactatt tggtatagag gaaagtcaga tagaaataat ccagactcac
    29101 tattgcatct taaaaaaatt cgggaatatc ttttagatgg agaaattcag aaagccgaag
    29161 aattgataaa gttaacaatg tttgctaccc caagagatca aagccactat gaattacttg
    29221 gggaactttg cattgagcat atagatattc agtcttgtgc tctttcattg tatgaaagag
    29281 agctagattt agatacagct atttctaatg ttgtgtttga gcctaatagt tgtaatttac
    29341 aaataaaaag agaatatttt acgagtttta ataagaatat tttatgttgc cgtatagtgt
    29401 catcagttca aaacacatta aatttaaaca ttaatttggg tagaaataaa cggtttaatg
    29461 acgaagtatc taaactggat tcaagtacaa ttttaatgtc ggcctctgct ggaggtagaa
    29521 aaggtgttca gtttaaagta gtatgtcatt ctaaggttac ggatggtgaa gtaagtgtat
    29581 tgggagagac aatagttatt cggaatgcta cagaggtatt tctttatctc aaatcaatga
    29641 cggattattg gggaaatata gatatttctt ctcttcaggg agaatttagt agtattgatt
    29701 actttacaga aaaagatgaa catgtaaaaa aatatcagga gcaatttaat agagttgatt
    29761 ttaaactaga ctatagtaaa ggttgtctta gcattccaac gaatctactt cttgaaaaca
    29821 ctaaaaagta tagtaactac ttgactaact tgttatttca ttatggaaga tatctgttaa
    29881 tatcgtctag tcaaccgaat ggtttacctg ccaatcttca aggaatatgg tgtgatgaat
    29941 taaatccaat ttggggttct aaatatacga ttaatattaa tactcaaatg aattattgga
    30001 tggtaggtcc atgtgattta ccagaagtag aatatccatt atttgatatg ctcgaaagaa
    30061 tgagagaacc gggaagacta accgctaaga aaatgtatgg agctagaggt tttacagcac
    30121 atcataatac ggatggtttt ggcgatacgg ctccccaatc tcatgccatg ggggctgcaa
    30181 tttgggtatt aactattcca tggttatgta ctcatatttg ggaacactat ttatatttcc
    30241 aagatgagcg tattcttacg gaacattttg aaatgataaa agaagcattt cttttctttg
    30301 aagattattt atttgaggtg gatggctact tgatgacagg tccaagtgtc tcaccggaaa
    30361 ataaatatcg cttaaaaaat ggtattgaag gaaatgcttg tctatcatct acaattgata
    30421 atcaaattct aagatatttt tgtgattcat gcattggcat tgcaaaacaa ttaggagaca
    30481 attcggattt tattagtcgt gtgaaggagt taaaaaagaa attacctaga acaaaaatag
    30541 gtagtaatgg gcaaatacaa gaatggctag aggattatga agaagtagag cctggtcata
    30601 gacacatttc acctctattt gggctttatc cttataatga gattgatatt cataaaactc
    30661 cggaattagc agaagcagct aaaatcacta tcaataggag attatcaaac gctaactttt
    30721 tatcttcaca ggatagggag caagcgatta ataattggtt agtaagtggt ttgcatgcta
    30781 gtacacaaac aggttggagt gctgcatggc tgattcattt ttttgcgaga ctatatcaag
    30841 gtgaacctgc ttataaccag attaatggtt tgttaaataa tgcgactctt ggcaatttat
    30901 ttcttgacca tccaccattt caaattgatg gtaatttagg tttggtgagt ggaatttgtg
    30961 aattattagt acagagccat cataattggt tatcactaat tccagcttta ccttctgctt
    31021 ggtcagaagg agaagtgaaa ggtttcagag taagaggagg atataaggta tcgtttgctt
    31081 ggaaaaatgg ggatataaca ttcctaaaat tggaaggagg aaacaaagat caaaaagtaa
    31141 gagtaagaat atatggcaaa aatactgatg tacaaaatat tgaattggta tttaattcag
    31201 aaaaaattat tgagttaaat ttttaggtat aagtcatgaa taaagaaaaa ataaaaagaa
    31261 aattaatcac aatattgttt gtatgtattg ggatgttatg ttttggattg ttagcaggag
    31321 ttaaggctga taatcgtgtt caaatgagaa cgacgattaa taatgaatcg ccattgttgc
    31381 tttctccgtt gtatggcaat gataatggta acggattatg gtgggggaac acattgaagg
    31441 gagcatggga agctattcct gaagatgtaa agccatatgc agcgattgaa cttcatcctg
    31501 caaaagtctg taaaccaaca agttgtattc cacgagatac gaaagaattg agagaatggt
    31561 atgtcaagat gttggaggaa gctcaaagtc taaacattcc agttttcttg gttattatgt
    31621 cggctggaga gcgtaataca gttcctccag agtggttaga tgaacaattc caaaagtata
    31681 gtgtgttaaa aggtgtttta aatattgaga attattggat ttacaataac cagttagctc
    31741 cgcatagtgc taaatatttg gaagtttgtg ccaaatatgg agcgcatttt atctggcatg
    31801 atcatgaaaa atggttctgg gaaactatta tgaatgatcc gacattcttt gaagcgagtc
    31861 aaaaatatca taaaaatttg gtgttggcaa ctaaaaatac gccaataaga gatgatgcgg
    31921 gtacagattc tatcgttagt ggattttggt tgagtggctt atgtgataac tggggctcat
    31981 caacagatac atggaaatgg tgggaaaaac attatacaaa cacatttgaa actggaagag
    32041 ctagggatat gagatcctat gcatcggaac cagaatcaat gattgctatg gaaatgatga
    32101 atgtatatac tgggggaggc acagtttata attttgaatg tgccgcgtat acatttatga
    32161 caaatgatgt accaactcca gcatttacta aaggtattat tcctttcttt agacatgcta
    32221 tacaaaatcc agctccaagt aaggaagaag ttgtaaatag aacaaaagct gtattttgga
    32281 atggagaagg taggattagt tcattaaacg gattttatca aggactttat tcgaatgatg
    32341 aaacaatgcc tttatataat aatgggagat atcatattct tcctgtaata catgagaaaa
    32401 ttgataagga aaagatttca tctatattcc ctaatgcaaa aattttgact aaaaatagtg
    32461 aggaattgtc tagtaaagtc aactatttaa actcgcttta tccaaaactt tatgaaggag
    32521 atgggtatgc tcagcgtgta ggtaattcct ggtatattta taatagtaat gctaatatca
    32581 ataaaaatca gcaagtaatg ttgcctatgt atactaataa tacaaagtcg ttatcgttag
    32641 atttgacgcc acatacttac gctgttgtta aagaaaatcc aaataattta catattttat
    32701 tgaacaatta caggacagat aagacagcta tgtgggcatt atcaggaaat tttgatgcat
    32761 caaaaagttg gaagaaagaa gaattagagt tagcgaactg gataagcaaa aattattcca
    32821 tcaatcctgt agataatgac tttaggacaa caacacttac attaaaaggg catactggtc
    32881 ataaacctca gataaatata agtggcgata aaaatcatta tacttataca gaaaattggg
    32941 atgagaatac ccatgtttat accattacgg ttaatcataa tggaatggta gagatgtcta
    33001 taaatactga agggacaggc ccagtccctt ttccaacacc agataaattt aatgatggta
    33061 atttgaatat agcatatgca aaaccaacaa cacaaagttc tgtagattac aatggagacc
    33121 ctaatagagc tgtggatggt aacagaaatg gtaattttaa ctctggttcg gtaacacaca
    33181 ctagggcaga taatccctct tggtgggaag tcgatttgaa aaaaatggat aaagttgggc
    33241 ttgttaaaat ttataatcgc acagatgctg agactcaacg tctatctaat tttgatgtga
    33301 ttctatatga caataataga aacgaagttg ctaagaaaca tgttaataat ttgtcgggtg
    33361 aatctgttag tctagatttc aaagaaaaag gagcaaggta tattaaagtt aaattactaa
    33421 cgagtggagt gcctttgagt ttagcagaag tagaggtttt tagagaatca gatggtaagc
    33481 aatctgaaga ggatatagat aaaataacag aagataaagt agtctctaca aataaggtag
    33541 ctactcaaag ttcaaccaat tatgagggtg tagctgcttt agcagttgat ggtaataaag
    33601 atggagatta cggacatcat tcggtgactc atactaaggc agattctaac gcttggtggc
    33661 aggtcgatct gggagaagag tttacggttt ctaaagttga tatttataat agaacagatg
    33721 ccgaacctca gcgtttatct aattttgatg ttatttttct atcttcatca ggagaagaag
    33781 tttttagaag acattttgat aaagtagttg atggtttgtt atctttaaaa gtaccttctg
    33841 taggggctaa gctagtcaaa atagaattaa aatcagcagc tattccgtta agtttagcgg
    33901 aagttgaagt ctatggttca aagagaactc cgaagaaact ttctaatatt gcattaacca
    33961 aagaaactcg acagagttca acggattaca atggtttttc tcgtctagca gttgatggaa
    34021 ataaaaacgg agattatggt catcattcag tgactcatac caaaggagat tctccttcat
    34081 ggtgggagat agatttagca caaaccgaag aattagaaaa gttaattatt tataatagaa
    34141 cagatgctga aattcagaga ttatcaaatt ttgatattat tatatatgat tcaaataatc
    34201 atgaagtttt taaacaacat attgacagtt tagaaagcaa taatctatcc atagacttaa
    34261 aagggctgaa gggaaaaaag gttagaattt ctttgagaaa cgcaggaatt cctttaagtt
    34321 tggcagaggt agaggtttat acttataagt aattttaaaa attatcatcg gtagtatgaa
    34381 agaaacagaa aaataagagg aaaatagtat gattcaacat ccacgtattg gtattcgtcc
    34441 gactattgat ggtcgtcgtc aaggtgtacg cgaatcactt gaagtacaaa caatgaacat
    34501 ggctaaaagt gtggcagatt tgatttcaag cacattgaaa tatccagatg gggaacctgt
    34561 ggaatgtgtg atttctccat ctaccattgg tcgtgttcca gaggctgcag cttcccatga
    34621 gttgtttaaa aaatcaaatg tttgcgcaac aattacagtt acaccatgct ggtgttatgg
    34681 tagtgaaact atggatatgt ctccagatat tcctcatgct atttggggat ttaatgggac
    34741 agaacgccca ggagctgtct atcttgcagc tgtactagct tcacatgctc aaaaagggat
    34801 tccagccttt gggatttatg gtagagatgt tcaggaagct aatgatacag atattccaga
    34861 agatgtcaaa gaaaaacttt tacgttatgc gcgtgcagct cttgcaactg gcttgatgag
    34921 agacactgct tacctatcaa tgggtagtgt ttcgatgggg attggtggtt ctattgtaaa
    34981 tccggatttc ttccaagaat acttaggaat gcgaaatgaa tcggtagata tgacggagtt
    35041 cacacgccgt atggaccgtg gtatttacga ccctgaagag tttgaacgtg ctatggtttg
    35101 ggtaaaagaa catatcaaag aaggagttga ccgaaatcgt gaggatttga ttctttctaa
    35161 agaagagaaa gaaaaacaat gggaatttgt tattaagatg ttcatgattg gacgtgactt
    35221 aatggttggt aacccaagac ttgctgaact tggttttgag gaagaagcag ttggtcacca
    35281 tgctttagta gctggtttcc aaggtcaacg tcagtggaca gaccattttc caaatggaga
    35341 ttttatggaa actttcctca atactcagtt tgactggaat ggtattcgaa aaccatttgt
    35401 atttgcgaca gagaatgatt cactaaatgg tgtgtctatg ctctttaatt atctactaac
    35461 aaatactcca caaatctttg ctgatgtgcg tacttattgg agtccagagg ctgttgaacg
    35521 tgtaacagga tatactttag agggtcgtgc tgcagctggc ttcttacatc taatcaactc
    35581 tggttcttgt acattggatg gtacaggtca agctactcga gatggcaaac ctgttatgaa
    35641 accattctgg gagttggatg aaagtgaagt acaggctatg cttgaaaata cagacttccc
    35701 accagcaaac cgcgagtact tccgtggagg aggattctca actcgtttct tgacgaaggg
    35761 ggatatgcca gtaacaatgg tacgtctcaa tcttctaaaa ggggttggtc cagtgctaca
    35821 aattgcagaa ggttacacac ttgaacttcc tgaagatgtt caccatactt tagataatcg
    35881 tacagattca ggatggccaa ctacttggtt tgctccacgt ttgacaggaa aaggtgcttt
    35941 caagtctgtc tatgacgtca tgaataattg gggagctaat cacggagcca taacatatgg
    36001 acacattgga gcagacttga ttaccttggc ttctatgttg agaattcctg tcaatatgca
    36061 taatgtacct gaggaagata tctttagacc taaaaattgg tccttatttg gaacagaaga
    36121 tctagaatca gcagactatc gtgcatgtca gttgttggga ccactacata aataaaactt
    36181 gtttatatag gaggtgaact tacgtccctc ctatcctttt aaaaagattt gttaaacaat
    36241 tcacaaataa ttgaaaacga atacaaaaag taatataatg atgttaaata gatagcgcgg
    36301 aggcgcagga ggaaaattat atggctatat tttatgttcc ggcagtcaac cttattggaa
    36361 aaggtgttgt aaatgaagtg ggtccttata tcaaggaact tggctataaa aaggcacttt
    36421 tggtgacaga taagtacatc gaaggcagtg atattttacc taagacttta aaaccactgg
    36481 atacagaagg aatcgaatat gtcatcttta gcgatgtaga gccaaaccct acttgtaaaa
    36541 atgtcacaga tggggtagct gctttgcaag aacatggctg tgactttatc atcagtcttg
    36601 gcgggggttc tccacaggat gcagctagtt gtatttctat catggctaca aatggtggaa
    36661 aaccacagga ttatgaaggg cttcataagt ctgctaaaaa aggtttgcca gttgtggcta
    36721 tcaatacaac ggcaggtaca tcagcagaaa ttaccattaa ctatgtgatt actgatgaag
    36781 aacgcaaggt taagatggta atggttgaca agaatagcct tgccttgatt tctgtcaatg
    36841 accctgaact catgctttcc aaacctaaag gcctgactgc tgctactggt atggatgctc
    36901 tgactcatgc tgttgaagct ttggtaacac ctggtgctta tgatgtaacc aagaaactgt
    36961 ctattggtgc tattgagctt atcaaggaat atcttcctcg tgctgtagaa aatggacatg
    37021 atattgaagc gcgtgaaggt atggtcaatg ccatcttcct tggtggtatg agctttaata
    37081 atgctggtct tggctatgtt cactcaatgg ctcaccaact cggtgcagta tataatttgc
    37141 cacatggcgt gtgctgtgcc atgttgctac cagttataga acgtgaaaat gctaaacgtg
    37201 taccagaagc tttccgcaat gttgccaaag ccttgggact tcatgtagaa ggtaaatcag
    37261 atcaagaatg tgccgattat gcgattgctg agattgagaa gctttctgag acagtaggta
    37321 ttcctaagaa actgactgaa cttggtattg aagaaaaaga tttcgacttt gaataccttt
    37381 ctaagaatgc cttgattgat gcctgcgcac caggaaatcc atttatgcca accttagaag
    37441 aaacgattgc cttttataaa gagttatttt agcaagagat tggacgacaa taggacaatt
    37501 cacctctaat tattgacatt tcaggaaaaa tcgcagtaaa aacggcacag ccttctttaa
    37561 atgtgctata atactagaaa aatacttgtg gaggttccat tatggcaata tttttcatga
    37621 tttttctgat tgtttgtgtg ctcctattgg tgatagtcac actgagtaca gtttatgtgg
    37681 ttcgtcagca gtcggtggcg attattgaac gctttgggaa ataccaaaag gttgctaata
    37741 gcggtattca tattcgcttg ccttttggga ttgactcgat tgcagcacgg attcagttgc
    37801 gcttgttgca aagtgatatt gtggttgaga ctaagaccaa ggacaatgtg ttcgttatga
    37861 tgaatgtagc gactcagtac cgtgtcaacg agcagagcgt gacagatgct tactataaac
    37921 tcatacgtcc agaatctcag attaaatctt atatcgaaga tgctcttcgc tcttctgttc
    37981 caaaattaac cttggatgaa ttgtttgaga aaaaagatga gattgccctt gaggttcaac
    38041 accaagtagc agaagaaatg accacttacg gctacattat cgtgaaaacc ttgattacca
    38101 aggtcgaacc agatgcagaa gttaagcaat ctatgaatga aatcaatgcg gcgcaacgta
    38161 agcgggtcgc agcacaagaa ttggcggaag ctgacaagat taaaattgtc actgcagctg
    38221 aagccgaagc agaaaaagac cgccttcatg gtgtggggat tgcccaacaa cgtaaggcga
    38281 ttgtggatgg attggcagag tctatcaccg aactcaagga agccaatgtt ggcatgacag
    38341 aagaacaaat catgtctatc ctcttgacca accagtattt ggataccttg aatacctttg
    38401 cctctaaagg aaatcaaacc atctttttac caaatactcc aaatggtgtg gatgatatcc
    38461 gtacacaaat cttgtcagcc cttcgcgctg agaagaaata atagactaat actcttcgaa
    38521 aatctcttca aactacgtca gcgtcgtctt gccgtataga tgttactgac ttcgtcagtt
    38581 ctatctgcaa cctcaaaaca gtgttttgag ctgacttcgt cagtcttatc tactacctca
    38641 aaacggtgtt ttgagcaacc tgtagctagt ttcctagttt gctctttgat tttcattgag
    38701 tataaagagc ttggcaacag gctctttttg tattgcgttt attaagaaaa acaaaaaaca
    38761 tcgatataca tttaactaaa aattattgta tgttatctcc cttaattagg aatgataagg
    38821 gaataactag aaagatttgt gaatacaaac tatttctgat atacaaaata tacagtaata
    38881 atgaatgatg ggagatggga tgaaagaatt tcaatttgag agaaagcagc gtttttcttt
    38941 gaggaaatat gcaataggag cttgttcggt cttgctagga acgagtttat tttttgctgg
    39001 tatgggtgct cagcctgtac aggatacaga aacgagttca gcactaattt caagtcatta
    39061 tttggatgag caggatttat ctgaaaagct gaaatctgag ttgcaatggt ttgaattaga
    39121 aaacaagctt ttgaacttat gggagcatta ggttactatg aaggatttgt tccttatgtt
    39181 tcaaatcaat accaaaacca agctgaagaa gaatacaaac cgctatctga taaatatatt
    39241 ttcgaaaaaa tcttaggaaa aacatatgca gccttcaaaa aagatcaaat taatgagcgt
    39301 gtcgagaaat taggtaagtt aaaacctatt acaataaatt acaacggaaa atcagaagta
    39361 attgatagta aagaaaaatt acaagagctt atgaataaag ccgttaaaga cgaagtggct
    39421 caaatatagt aaaatgaaa
//
LOCUS       DBEJAQ010000016        39197 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.016, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000016 DBEJAQ010000000
VERSION     DBEJAQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.016"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..748
                     /locus_tag="ACL297_000877"
     CDS             <1..748
                     /locus_tag="ACL297_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071162.1"
                     /translation="SLILPSLFSIISKLFLFLSIGLLSIEQGQVVIYVILLNYFILVM
                     GILDNHASNFYFKSSRMSQKRFRSSLFILLRFCWLNKGLTFSFILISILLSYLFWYMM
                     TMLSIDDTKIPILFLYTSIYITLMEAIIGNHKSSIGVDLALLQLSALNHSKMNFFFKN
                     AQLMMLTHLISFLQFSLLLCLIPVTSYSNDIKSILLNLLLIPFNYFIAYVYYKKALFL
                     IKGTVSLLRWSFLVLYCFLIIFVILVGGIR"
     gene            745..1227
                     /locus_tag="ACL297_000878"
     CDS             745..1227
                     /locus_tag="ACL297_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HGR0071163.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYLLLILAALIEGIKL
                     "
     gene            1224..1820
                     /locus_tag="ACL297_000879"
     CDS             1224..1820
                     /locus_tag="ACL297_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0071164.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
     gene            1801..2292
                     /locus_tag="ACL297_000880"
     CDS             1801..2292
                     /locus_tag="ACL297_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071165.1"
                     /translation="MKFQTNNSLYKLMNLIIYLFLLYLFIDLLMKSGILDEQIDKLSE
                     LSNLFTIKIETILTVISVVFTFIATLIEYGFSLFLITLFFDKNKRNFGDILLSKNITL
                     SFSILLCYLFNPSDIKFAYFSVLTVLLIGITNYARFKNLRLTVVFSVPFLFNAIMSII
                     NSF"
     gene            2672..4825
                     /gene="comA"
                     /locus_tag="ACL297_000881"
     CDS             2672..4825
                     /gene="comA"
                     /locus_tag="ACL297_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="HGR0071166.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEERKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            4838..6187
                     /gene="comB"
                     /locus_tag="ACL297_000882"
     CDS             4838..6187
                     /gene="comB"
                     /locus_tag="ACL297_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence pheromone export protein ComB"
                     /protein_id="HGR0071167.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            6357..7064
                     /gene="purC"
                     /locus_tag="ACL297_000883"
     CDS             6357..7064
                     /gene="purC"
                     /locus_tag="ACL297_000883"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGR0071168.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            7121..10846
                     /locus_tag="ACL297_000884"
     CDS             7121..10846
                     /locus_tag="ACL297_000884"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGR0071169.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            10939..12381
                     /gene="purF"
                     /locus_tag="ACL297_000885"
     CDS             10939..12381
                     /gene="purF"
                     /locus_tag="ACL297_000885"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGR0071170.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            12418..13440
                     /gene="purM"
                     /locus_tag="ACL297_000886"
     CDS             12418..13440
                     /gene="purM"
                     /locus_tag="ACL297_000886"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGR0071171.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            13437..13982
                     /gene="purN"
                     /locus_tag="ACL297_000887"
     CDS             13437..13982
                     /gene="purN"
                     /locus_tag="ACL297_000887"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGR0071172.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            14066..14575
                     /locus_tag="ACL297_000888"
     CDS             14066..14575
                     /locus_tag="ACL297_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGR0071173.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            14600..16147
                     /gene="purH"
                     /locus_tag="ACL297_000889"
     CDS             14600..16147
                     /gene="purH"
                     /locus_tag="ACL297_000889"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGR0071174.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQEFIEAADKYGLTMVFT
                     GVRHFRH"
     gene            16269..17531
                     /gene="purD"
                     /locus_tag="ACL297_000890"
     CDS             16269..17531
                     /gene="purD"
                     /locus_tag="ACL297_000890"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGR0071175.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            17788..17904
                     /locus_tag="ACL297_000891"
     CDS             17788..17904
                     /locus_tag="ACL297_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGR0071176.1"
                     /translation="MKERLDDNPIVQGNWKTLGFQFRHETPLVAAAVPHKLR"
     gene            17934..18422
                     /gene="purE"
                     /locus_tag="ACL297_000892"
     CDS             17934..18422
                     /gene="purE"
                     /locus_tag="ACL297_000892"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGR0071177.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            18409..19482
                     /gene="purK"
                     /locus_tag="ACL297_000893"
     CDS             18409..19482
                     /gene="purK"
                     /locus_tag="ACL297_000893"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGR0071178.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVGEF"
     gene            19505..21892
                     /locus_tag="ACL297_000894"
     CDS             19505..21892
                     /locus_tag="ACL297_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGR0071179.1"
                     /translation="MFEKIKGINIKSGIFEDETKLELFEGNFEGTNPVQNDRASLLFG
                     RNGSGKSTIARGINQLKNGEIGTDRVSFIDKNNNNIVLSDTERKSIFVFNEHYVDQKV
                     KIAQEGLDTIVILGEQVDIDEELDRLRTQLSESQIESQDYYAEYEEYLDEKNEKSPDF
                     WKKEMTDSLKGVGNWAERDREIKGNRAASPVHNNTFQNFVDLQPILDKNELEVEFNNK
                     KARYFSIRDSAVTINNELSLPDINFDSNELSTLLSEKIEEPELNSRDKYLLTLLSDST
                     KGERHLREVKDFFEDEHQKKCPFCTQSVSEDVKVELTNGITKLLSRAVEEHQSALRGK
                     KIDEINQDFSGYEQIDPILIQSYQNSINALNAKFNEINSIIDKKIDNPYNIVELPNIS
                     FSQELSQAEHDIEKINQAIIKHNSEISGIQKLKVDLLQINNELAFYEIQDAYKKFQEK
                     TNKKAICENNYNNSSKRVKDYEKQISDLEDKKLNIDIAVDEINKSLNYIFFSKNRLAI
                     QNQNGKYYLLSRGKSVVPSRVSVGERNALALCYFFTEIIQQRELADAYSHEYFIVIDD
                     PISSFDMENKVGITSYLKYCLTRFFKGNSNTRVLLMTHDKQTIYDFDIFLKEIMESCK
                     EEEGGQKSKYKKLELVSGKLQEFKTSTHDYTELLEIVFGYALGNSTPTSESFVGNAMR
                     KILEAYGSFNYKKGIAELTTDPLIVEKIDKEYRTYFENLMYRLVLNGESHFKDPVKTL
                     SIDFFDTISDEERKKTARDLLVLLYLLDDLHVLKHLEGVSNAENRLEQWKCEILE"
     gene            22065..23363
                     /gene="purB"
                     /locus_tag="ACL297_000895"
     CDS             22065..23363
                     /gene="purB"
                     /locus_tag="ACL297_000895"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGR0071180.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEINEIFNPIYYTKRVDDIFERLGLGD"
     gene            complement(23418..27356)
                     /gene="strH"
                     /locus_tag="ACL297_000896"
     CDS             complement(23418..27356)
                     /gene="strH"
                     /locus_tag="ACL297_000896"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored beta-N-acetylhexosaminidase StrH"
                     /protein_id="HGR0071181.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPRLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(27671..28387)
                     /locus_tag="ACL297_000897"
     CDS             complement(27671..28387)
                     /locus_tag="ACL297_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGR0071182.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            28735..30522
                     /locus_tag="ACL297_000898"
     CDS             28735..30522
                     /locus_tag="ACL297_000898"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGR0071183.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            30519..30995
                     /locus_tag="ACL297_000899"
     CDS             30519..30995
                     /locus_tag="ACL297_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0071184.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            31023..31928
                     /locus_tag="ACL297_000900"
     CDS             31023..31928
                     /locus_tag="ACL297_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGR0071185.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            31915..32730
                     /locus_tag="ACL297_000901"
     CDS             31915..32730
                     /locus_tag="ACL297_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGR0071186.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALTLSALGHFALGM"
     gene            32737..33141
                     /locus_tag="ACL297_000902"
     CDS             32737..33141
                     /locus_tag="ACL297_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGR0071187.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIGGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            33439..34605
                     /locus_tag="ACL297_000903"
     CDS             33439..34605
                     /locus_tag="ACL297_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGR0071188.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKKLVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALDCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            34721..35758
                     /locus_tag="ACL297_000904"
     CDS             34721..35758
                     /locus_tag="ACL297_000904"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HGR0071189.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            35965..36819
                     /locus_tag="ACL297_000905"
     CDS             35965..36819
                     /locus_tag="ACL297_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGR0071190.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            36969..37226
                     /locus_tag="ACL297_000906"
     CDS             36969..37226
                     /locus_tag="ACL297_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HGR0071191.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADLVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(37268..37585)
                     /locus_tag="ACL297_000907"
     CDS             complement(37268..37585)
                     /locus_tag="ACL297_000907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071192.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDRTDEVSNHIYGKVKLTWFEEI
                     FEEYKSPHNKTHTIKVFMYCPPKVRQKKSNFWGQYISIPDTMRFFNFKDFLQLLLTHI
                     KLF"
     gene            complement(37657..37935)
                     /locus_tag="ACL297_000908"
     CDS             complement(37657..37935)
                     /locus_tag="ACL297_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGR0071193.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNA"
     gene            complement(37925..38080)
                     /locus_tag="ACL297_000909"
                     /pseudo
     CDS             complement(37925..38080)
                     /locus_tag="ACL297_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
     gene            38157..38921
                     /locus_tag="ACL297_000910"
     CDS             38157..38921
                     /locus_tag="ACL297_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HGR0071194.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
BASE COUNT        11156 a         7289 c         8891 g        11861 t
ORIGIN      
        1 ttctttaatt ctacctagtt tgttttcaat tataagtaaa ttgttcttat ttttatctat
       61 aggactattg tctatagaac aaggacaagt tgtaatttat gtcatactac tcaattattt
      121 tattttagtc atggggatat tagataatca tgcgagtaat ttctatttta aaagcagtcg
      181 aatgagtcaa aaaagatttc gtagcagtct atttatattg cttcgttttt gttggttaaa
      241 taaagggtta actttttctt ttattttgat tagtattctt ctttcctatc tattttggta
      301 tatgatgacg atgttatcaa ttgatgacac aaaaatacca atattatttc tttatacctc
      361 aatatatatt acattaatgg aggcaataat agggaatcat aaatcctcaa ttggggtaga
      421 tctagcttta cttcaattat ctgctcttaa tcattctaaa atgaatttct tttttaagaa
      481 tgcacaacta atgatgttga cgcatttaat ctctttctta caattttcat tgttgttatg
      541 tttgattccg gtgacaagtt atagtaatga tataaaaagt attttgttga atttgttatt
      601 gattccattc aattatttta tagcctatgt ctattataag aaagctctat ttttaattaa
      661 aggtacagtt agtttattga gatggagttt tctggtttta tattgttttc tgataatatt
      721 tgtaatttta gtagggggca ttagatgaga aaaagtggtt ttctgattta ctatgtgtta
      781 ttttgtatag tctgtttttt actaggttta cttttaagct ttatattaaa aaatgagact
      841 tttgctacta tacctccgaa actggaatta caatttacat tgacagtttt acaggcaaat
      901 tctacaaatt ttttgctata tctattctgt tttccgattt cgcccttatt gcaacttttt
      961 gatttgctat ctttaggaat acaattgggt atatctggtc agatgattgg aatagtagct
     1021 acaataaaag gaatattacc tcatgctctt attgaaatac cgaattttat ttggtatcaa
     1081 ggtatgagtc agtatctttt ataccgatta ttgaaggata ggggaattaa aaatatactt
     1141 atttatataa aaagtaattt aaaatggtat atcatttctt atcttttatt aattttagca
     1201 gcgttgatag agggaattaa attatgaaaa ttgaaaatga atctaaaagt tatgataaat
     1261 gtatattgaa aaatatatca ctagagattg ataagggaat ttacagagtt gaaggagaga
     1321 atggttcagg aaagagcacc cttctacaac ttttagctgg gctagagact tttgatagtg
     1381 gtttaaagaa ttgtgtctcc aaagatgtac tataccttga taccaatcag cttggggttg
     1441 ttccatttac tatagaagaa aatttgcagt tgttgtgtga ttcttttcaa atccatctat
     1501 catttgagga taaagaacaa attaatactt tttttgataa taaaatcgga gataactata
     1561 tgacagcatc tactggaaca aaattcaaat taggcttatc cttaattttt gctaaaaatt
     1621 gggattatat ttttattgat gagactttat caacagtgga tgcaagaagt attgatatga
     1681 ttgctaaacg cttaatttcc atgaaagaaa gctctactat tatatatgta tctcataatt
     1741 tattgaatca agaattgatt agcaaatctc aaaggatttt aatagaggaa ggacgggttt
     1801 atgaaatttc aaacaaataa tagtttatac aaattaatga atctgattat ttatcttttt
     1861 cttttatatt tgtttattga tttactgatg aagtcgggaa ttttagatga acaaattgat
     1921 aaattatctg aactatctaa tctttttaca attaaaatag aaacaatatt gacagtgatt
     1981 tctgttgttt tcacatttat agcaactctt attgaatatg gattttctct ctttctgatt
     2041 acattatttt ttgataagaa taagaggaac tttggagata ttcttttatc taaaaatata
     2101 accttaagtt ttagcatttt actttgttat ttatttaatc catcggatat aaagtttgcc
     2161 tatttcagcg tattgacagt attactaatt gggataacaa attatgcgag atttaaaaat
     2221 ttaagattga cagtagtgtt ttctgtacca tttttattta atgctataat gtctattata
     2281 aatagttttt agaaatacta aaattttttt ctttacttaa taatgatatg aaatcatttc
     2341 ggttttgtta acttgtaatt cttatatttt ctaggtagaa cgtttgttct gcctattttt
     2401 ttatccaaaa agtgcagttg ggagggagat aggctcattt gggaaggaag tccagttttt
     2461 gtttagtgat tggggtaaga tagttgttat cagatgagtt tatactcttc gaaaatcaaa
     2521 ttcaaaccac gtcaacgtcg ccttgccgta tatatgtgac tgacttcgtc agtcctatct
     2581 acaacctcaa aacagtgttt tgagcagcct gcggctagtt tcctagtttg ctctttgatt
     2641 ttcattgagt attagggaaa aggagatgaa tatgaaattt gggaaacgtc actatcgtcc
     2701 gcaagtggat cagatggact gcggtgtagc ttcattagcc atggtttttg gctactatgg
     2761 tagttattat tttttggctc acttgcgaga attggctaag acgaccatgg atgggacgac
     2821 ggctttgggc ttggtcaagg tggcagagga gattggtttt gagacgcgag ccattaaggc
     2881 ggatatgacg ctttttgact tgccggattt gacttttcct tttgttgccc atgtgcttaa
     2941 ggaagggaaa ttgctccact actatgtggt gactgggcag gataaggata gcattcatat
     3001 tgccgatcca gatcccgggg tgaagttgac taaactgcca cgtgagcgtt ttgaggaaga
     3061 atggacagga gtgactcttt ttatggcacc tagtccagac tataagcctc ataaggaaca
     3121 aaaaaatggt ctgctctctt ttatccctat attagtgaag cagcgtggct tgattgccaa
     3181 tatcgttttg gcaacactct tggtaaccgt gattaacatt gtgggttctt attatctgca
     3241 gtctatcatt gatacctatg tgccagatca gatgcgttcg acactaggga ttatttctat
     3301 tgggctagtc atcgtctaca tcctccagca aatcttgtct tacgctcagg agtatctctt
     3361 gcttgttttg gggcaacgct tgtcgattga cgtgattttg tcctatatca agcatgtttt
     3421 tcacctccct atgtccttct ttgcgacacg caggacaggg gagatcgtgt ctcgttttac
     3481 agatgctaac agtatcatcg atgcgctggc ttcgaccatc ctttcgattt tcctagatgt
     3541 gtcaacggtt gtcattattt cccttgttct attttcacaa aataccaatc tctttttcat
     3601 gactttattg gcgcttccta tctacacagt gattatcttt gcctttatga agccgtttga
     3661 aaagatgaat cgggatacca tggaagccaa tgcggttctg tcttcttcta tcattgagga
     3721 catcaacggt attgagacta tcaagtcctt gaccagtgaa agtcagcgtt accaaaaaat
     3781 tgacaaggaa tttgtggatt atctgaagaa atcctttacc tatagtcgag cagagagtca
     3841 gcaaaaggct ctgaaaaagg ttgcccatct cttgcttaat gtcggcattc tctggatggg
     3901 ggctgttctg gtcatggatg gcaagatgag tttggggcag ttgattacct ataatacctt
     3961 gctggtttac tttaccaatc ctttggaaaa tatcatcaat ctgcaaacca agcttcagac
     4021 agcgcaggtt gccaataacc gtctaaatga agtgtatcta gtagcttctg agtttgagga
     4081 gaggaaaaca gttgaggatt tgagcttgat gaagggagat atgaccttca agcaggttca
     4141 ttacaagtat ggctatggtc gagacgtctt gtcggatatc aatttaaccg ttccccaagg
     4201 gtctaaggtg gcttttgtgg ggatttcagg gtcaggtaag acgactttgg ccaagatgat
     4261 ggttaatttt tacgacccaa gtcaagggga gattagtctg ggtggtgtca atctcaatca
     4321 gattgataaa aaagccctgc gccagtacat caactatctg cctcaacagc cctatgtctt
     4381 taacggaacg attttggaga atcttctttt gggagccaag gaggggacga cacaggaaga
     4441 tatcttacgg gcggtcgaat tggcagagat tcgagaggat atcgagcgca tgccactgaa
     4501 ttatcagaca gaattgactt cggatggggc agggatttca ggtggtcaac gtcagagaat
     4561 cgctttggcg cgtgctctct tgacagatgc gccggtcttg attttggatg aggcgactag
     4621 cagtttggat attttgacag agaagcggat tgtcgataat ctcattgctt tggacaagac
     4681 cttgattttc attgctcacc gcttgactat tgctgagcgg acagagaagg tggttgtctt
     4741 ggatcagggc aagattgtcg aagaaggaaa gcatgctgat ttgcttgcac agggaggctt
     4801 ttacgcccat ttggtcaata gctagaaaga ggagaggatg aaaccagaat ttttagaaag
     4861 tgcggagttt tataatcgtc gttaccataa tttttccagt agtgtgattg tacccatggc
     4921 ccttttgctc gtgtttttac ttggctttgc aactgttgca gagaaggaga tgagtttgtc
     4981 cactagagct actgtcgaac ccagtcgtat ccttgcaaat atccagtcaa ctagcaacaa
     5041 tcgtattctt gtcaatcatt tggaagaaaa taagctggtt aagaaggggg atcttttggt
     5101 tcaataccaa gaaggggcag agggtgtcca agcggagtcc tatgccagtc agttggacat
     5161 gctaaaggat caaaaaaagc aattggagta tctgcaaaag agcctgcaag aaggggagaa
     5221 ccactttcca gaggaggata agtttggcta ccaagccacc tttcgcgact acatcagtca
     5281 agcaggcagt cttagggcta gtacatcgca acaaaatgag accatcgcgt cccagaatgc
     5341 agcagctagc caaacccaag ccgaaatcgg caacctcatc agtcaaacag aggctaaaat
     5401 tcgcgattac cagacagcta agtcagctat tgaaacaggt acttccttgg ccggtcagaa
     5461 tctagcctac tctctttacc agtcctacaa gtctcagggc gaggaaaatc cccaaactaa
     5521 ggttcaggca gttgcacagg ttgaagcaca gatttctcag ttagaatcta gtcttgctac
     5581 ttaccgtgtc cagtatgcag gttcaggtac ccagcaagcc tatgcgtcag ggttaagcag
     5641 tcaattggaa tcccttaaat cccaacactt ggcaaaggtt ggtcaggaat tgacccttct
     5701 agcccagaaa atcttggagg cagagtcagg taagaaggta cagggaaatc ttttagacaa
     5761 ggggaaaatt acggcaagtg aggatggggt gcttcatctt aatcctgaga ccagtgattc
     5821 tagcatggtt gcagaaggtg ccctactagc ccaactttat ccatctttgg aaagagaagg
     5881 gaaagccaaa ctcacagcct atctaagttc aaaagatgta gcaagaatca aggtcggtga
     5941 ttctgttcgc tatactacga ctcatgatgc cgggaatcaa ctttttctag attctactat
     6001 tacaagtatt gatgcgacag ctactaagac tgagaaaggg aatttcttta aaatcgaggc
     6061 ggagactaat ctaacttcgg agcaggctga aaaacttagg tacggggtgg aaggccgtct
     6121 acagatgatt acgggcaaga aaagttatct acgttattat ttggatcaat ttttgaacaa
     6181 agagtaatgt tcgtgttttt agagttaaat aatttttaaa ctgtgagaaa gattcttctt
     6241 gcagtttttt ctttacaatt tttgaaaaac atctgctatt tattcggtta aattcttgtg
     6301 ttttttggtt ttttgtggta aaatgtgctc aagtaatacg aaaggcgaac tttaaaatgt
     6361 caaaacaatt gatctattcg ggaaaagcta aagatatcta tacaactgag gatgaaaatc
     6421 ttattatttc aacttacaag gaccaggcga ctgctttcaa tggtgtcaag aaggagcaga
     6481 ttgcaggtaa gggagtcttg aataatcaga tttcatcttt tatttttgag aaattaaatg
     6541 cggctggtgt ggcgactcac tttgtggaga aactttcaga cacggaacaa ctcaataaaa
     6601 aggttaagat tattcctttg gaagtcgtgc tccgcaacta tactgctggt tccttttcaa
     6661 aacgttttgg tgtggatgag ggaatcgcct tggagactcc gattgtcgaa ttttactaca
     6721 aaaatgatga tttggatgat ccatttatca atgatgagca tgtaaaattc ctacagattg
     6781 cggatgacca gcagattgcc tacttgaagg aagaaacgcg tcgtatcaat gaactattga
     6841 aagtctggtt tgctgagatt gggcttaaat tgattgactt taagctagag ttcggttttg
     6901 acaaggatgg caagattatc ttggcagacg aattttcacc agataactgc cgcttgtggg
     6961 atgctgatgg caaccacatg gataaggatg ttttccgtag aggattggga gaactaaccg
     7021 acgtttatga gattgtttgg gaaaagttgc agggtttaaa ataacaacct caaggctgtt
     7081 tgggaatatt gcaagagctg aaataaagga ataagaattg atggataaac gtatttttgt
     7141 tgaaaaaaag gctgattttc aggtcaagtc agagagtttg gttagagagc tccagcacaa
     7201 cttgggactg tcaagcttga aaagtattcg tattgtgcaa gtatatgatg tatttgactt
     7261 ggttgaggac ttgtttgcac ctgcagagaa gcacattttc tctgagcagg taaccgacca
     7321 tgttttagat gaagtatctg tgcaggcgga tcttgctaac tatgctttct ttgccattga
     7381 aagtctacca gggcagtttg accagcgtgc agcttcgtca caggaagcct tgcttttgtt
     7441 gggaagttcg agtgacgtga cagtcaacac agcccaactt tacttggtga ataaagatat
     7501 tgatgcgact gagttggaag ctgtcaaaaa ctacctgctc aatccagttg attctcgttt
     7561 caaggatatc acgataggga ttgccaagca ggagttttca gagtcagaca agaccattcc
     7621 aaaattgcct ttctttgaaa gctatacagc agaagacttt gctcgctaca aggccgaaca
     7681 agggatggcc atggaagtgg atgatttgct ctttatccaa gactacttta agtcaatcgg
     7741 gcgcgtgcca actgagactg aactcaaggt tttggacact tactggtctg accactgccg
     7801 tcatacgact tttgagacag agttgaaaca catcgacttt tcagcttcta aatttcaaaa
     7861 gcaattgcag tcaacctatg acaagtatat tgccatgcgc gaggaattag gtcggtctga
     7921 aaaaccacaa accttgatgg atatggcgac tattttcggt cgttatgagc gtgctaatgg
     7981 acgattggat gatatggaag tctctgacga aatcaatgcc tgctcagttg aaattgaagt
     8041 ggacgttgat ggtgtcaagg aaccttggct cctcatgttt aaaaacgaaa ctcacaacca
     8101 tccaacagaa attgagccat ttggtggagc ggctacctgt atcggtggag ctattcgtga
     8161 tccgttgtca ggccgttcct atgtttacca agccatgcgt atttcaggtg ctggtgatat
     8221 tacagcaccg atttcggaaa ctcgtgctgg gaaattgcca caacaagtca tttctaaaac
     8281 agcaactcat ggttattctt catatggtaa ccagattggg cttgccacaa cctacgttcg
     8341 tgaatacttc cacccaggct ttgtagctaa acgtatggaa cttggtgccg ttgttggtgc
     8401 ggctcccaag ggcaatgttg tccgtgaaaa acctgaagca ggtgatgtga tcatccttct
     8461 cggaggtaaa acaggtcgtg atggtgtcgg tggtgcgacg ggctcttcta aggttcaaac
     8521 agttgagtct gtagagactg ctggtgctga ggttcaaaaa ggaaatgcca tcgaagaacg
     8581 caagattcag cgcctcttcc gtaatggcaa tgtcactcgt ctgatcaaga agtccaatga
     8641 ctttggggca ggcggtgtct gtgtggctat cggtgaattg gcagacggtc ttgaaatcga
     8701 cctcaacaag gtgcctctta aataccaggg cttgaatggt acagaaattg ccatctctga
     8761 atcacaagaa cggatggcgg tcgtggttcg tcctgaagat gtggatacct tcgttgccga
     8821 atgtaacaaa gaaaatattg atgctgttgt ggtggcgaca gtaactgaaa aaccaaatct
     8881 tgtcatgcac tggaatggtg agacaatcgt tgacttggag cgtcgtttcc ttgacatcaa
     8941 tggtgtgcgc gtggttgtcg atgccaaagt tgtggacaag gatgtcaaac tcccagaaga
     9001 gcgtcaaaca tctgctgaaa cactggaatc agataccctt acggttctat ctgacctcaa
     9061 ccatgcaagt caaaaaggat tacagactat ctttgactgc tctgttggac gctcaacggt
     9121 taatcaccca cttggtggtc gttaccaact cacaccaact gaggcatctg tgcagaaatt
     9181 gccagttcaa cacggtgtga ctcatactgc gtcggtcatt gctcaaggtt tcaacccata
     9241 tgtagctgaa tggtctccat accacggtgc tgcttatgcg gttatcgaag caactgctcg
     9301 tttggtggct gctggtgcca actggtccaa ggctcgtttc tcttaccaag aatatttcga
     9361 gcgtatggac aagcaagcag agcgttttgg tcagccagta gccgctcttc taggctctat
     9421 tgaagctcag attcaacttg gcttgccatc tatcggtggt aaggactcca tgtctggtac
     9481 ctttgaagaa ttgaccgttc cgccaacctt ggttgccttt ggggtgacga cggcagatag
     9541 ccgtaaggtg ctctctccag aatttaaagc tgttggggaa aatatctact acatcccagg
     9601 tcaagccctc tctgcagaga ttgattttga cttgattaag aaaaattttg ctcagtttga
     9661 agcaagccaa gctgaccata aagtgacatc tgcatcagct gtcaaatacg gtggtgtagt
     9721 tgaaagtttg gctcttgcta cctttggaaa ctatattggt gcagaggtga ccttgcctga
     9781 acttaaaaca gctttgacag ctcaattagg cggctttgtc ttcacatctc ctgaagaaat
     9841 tgctggagta gagaagattg gacaaacgaa agcagacttt acactgactg tcaacggtgt
     9901 gaagctagat ggacacaagc ttgacagtgc atttcaaggg acattggaag aagtttaccc
     9961 aacagaattt acccaagcga aagaactaga agaagtacca gctgtggcat cagatgttgt
    10021 gattaaagcc aaagaaaagg ttgaaaaacc tgtggtttac atcccagtct ttccaggaac
    10081 caactcagaa tatgattcag ctaaggcctt cgaaaaagaa ggtgcagagg tcaatttggt
    10141 gccattcgtg accttgaatg aagaagctat tgtcaagtca gttgaaacta tggttgacaa
    10201 tatcgacaag actaatattc tcttctttgc tggtggattc tcggctgcgg atgaaccaga
    10261 tggttcagct aagtttatcg tcaatatcct gcttaatgaa aaagtgcgtg tggctattga
    10321 tagctttatc gcccgtggtg gtttgattat cggtatttgt aatggattcc aagccttagt
    10381 caaatcgggt ctcctaccct acggaaactt tgaagatgct aacaatacta gcccaaccct
    10441 cttctacaat gatgccaacc aacacgtggc caagatggtg gaaactcgca ttgccaatac
    10501 caactcacca tggttggctg gtgtgcaagt gggcgatatc cacgctattc ctgtttcgca
    10561 cggtgaaggg aagtttgtcg tgacggctga ggaatttgca gagctccgtg acaatggaca
    10621 aattttcagc caatacgttg actttaacgg taaaccaagt atggattcta agtacaatcc
    10681 gaatggttct gtccatgcca tcgaaggaat taccagcaag aatggtcaaa tcatcggtaa
    10741 gatgggccac tcagaacgtt atgaggatgg tcttttccaa aatatcccag gcaataaaga
    10801 ccaacacctg ttcgcatcag cggttaaaca tttcactgga aaataagact tacagatttt
    10861 ctaatagata gtatcagtaa tgtaaaagtc atgtaaatct agctcttgat gattacaaat
    10921 gaaaattagg tataaaaaat gacatacgaa gtaaaatctc ttaatgaaga atgtggtgtt
    10981 ttcggtattt ggggacatcc agatgctgct aagttgacct attttggact ccacagtctt
    11041 caacaccgtg gtcaggaggg ggcaggaatc ctctccaatg atcaaggaca actgaagcgc
    11101 catcgtgaca tggggctttt atcagaagtt ttcagaaatc cagccaattt ggataaattg
    11161 acaggagctg gtgcgattgg gcatgtgcgt tatgcgactg ctggcgaagc ttctgttgat
    11221 aacatccagc ccttcctctt ccgttttcac gatatgcagt ttggcttggc tcataatgga
    11281 aatctgacca atgcagcctc tctcaagaaa gaactggaac aaagaggagc aattttcagc
    11341 gcgacttcgg actctgaaat cttggctcac ctcattcgtc gcagtcataa tcctagcctg
    11401 atgggcaaaa tcaaggaagc gctcagcctt gtcaaaggtg gttttgccta tatcttgctg
    11461 tttgaggaca agttgattgc ggctcttgac cctaatggct ttcgtccgct ttctatcggg
    11521 aaaatggcca acggagcggt ggttgtttcc tctgaaacct gtgcttttga ggtcattggt
    11581 gccgagtgga ttcgtgattt gaagccaggt gagattgtga tcattgatga cgagggcatt
    11641 cagtatgaca gctatacaga tgatactcag ttggcgattt gttctatgga gtatatctac
    11701 tttgctcgcc ctgattctaa tatccacggt gtcaatgtcc atacggcacg taagagaatg
    11761 ggagcgcaat tggcgcgaga atttaagcat gaggcagata ttgtagttgg tgtgcccaat
    11821 tcttccctaa gcgcggctat gggatttgcg gaagaatcag gcttaccaaa tgaaatgggt
    11881 ctgatcaaaa accaatacac ccagcgaact tttatccaac cgactcaaga attgcgggag
    11941 caaggagtgc ggatgaaact gtctgctgtt tcgggtgttg tcaaaggcaa acgtgtggtc
    12001 atggtggatg attccattgt acgtggaaca acctctcgtc gtatcgttca gctcttgaaa
    12061 gaagcgggtg cgactgaggt tcacgttgcc attggaagtc ctgcactagc gtatccatgt
    12121 ttctacggga ttgatatcca gacccgtcag gagctgattg cagccaatca tacggtcgaa
    12181 gaaactcgcc aaatcattgg tgcggacagt ctgacttatc tttcaattga tggcttgatt
    12241 gagtcgattg gtatcgaaac agatgcgccg aacggtggtc tctgtgtcgc ttactttgac
    12301 ggtgactacc caacgcctct ctatgactac gaagaagact atcgtagaag tttggaagaa
    12361 aagaccagtt tttacaagta ggcgacagat tctccattaa agaaaaggaa aaaacaaatg
    12421 acaaataaaa atgcatatgc ccagtcgggt gtggatgttg aagcgggtta tgaagttgtt
    12481 gaacggatta aaaagcacgt ggcccgtacg gagcgtgcag gtgtcatggg agctcttggt
    12541 ggctttggtg gtatgtttga cctttccaag actggggtta aagaacccgt cttgatttca
    12601 gggactgacg gtgtcggaac caagctcatg ttggctatca agtacgacaa gcacgatacc
    12661 atcgggcagg actgtgtggc catgtgtgtc aacgacatca ttgctgcagg tgcggaaccc
    12721 ctctattttc tcgactacgt agcgacaggg aagaatgaac cagctaagct agaacaagtg
    12781 gttgctggtg tggcagaagg ttgtgtgcag gctggtgctg ccctcatcgg tggggaaacg
    12841 gctgaaatgc cgggcatgta cggcgaagac gactatgact tggctggttt tgcggtcggt
    12901 gtggctgaaa aatctcaaat cattgacggt tcaaaggtgg tagagggaga tgttcttctc
    12961 ggacttgctt caagtgggat tcactcaaat ggttactctt tggttcgtcg tgtctttgcg
    13021 gattacacag gtgaggaagt cctaccagaa ttggaaggca agaaacttaa ggaagttcta
    13081 cttgagccga ctcgtatcta tgtcaaggct gtcttgccgc tcatcaaaga agagttggtc
    13141 aacggcattg cccacatcac aggtggtggc tttatcgaaa atgtccctcg tatgtttgca
    13201 gatgacctag ctgctgaaat tgatgaaagc aaagttccag tgcttccaat tttcaaagcc
    13261 cttgaaaaat atggtcagat taaacacgaa gaaatgtttg aaatcttcaa tatgggtgtg
    13321 ggacttatgt tggcggtcag ccctgaaaat gtagagcgtg taaaagaatt gttggatgaa
    13381 gcagtctatg aaattggtcg catcgtcaag aaagaaaacg aaagtgtcat tatcaaatga
    13441 aaaaaatagc ggtttttgcc tctggtaatg gctcaaattt tcaggtgatt gccgaagaat
    13501 ttccagtgga gtttgtcttt tcagaccatc gtgatgccta tgtgcttgag cgtgcaaagc
    13561 agctcggcgt tctgtcctat gcttttgaac tcaaggagtt tgagagcaag acagactacg
    13621 aagcagccct tgtcgaactc ttggaagaac accagattga cttggtttgc ctagcaggct
    13681 acatgaaaat cgttggacca accttattgt cggcttatga aggtcggatt gtcaacattc
    13741 atccagccta cttgccagaa tttccaggag ctcatgggat tgaggatgct tggaatgctg
    13801 gcgtgggtca gtctggtgtg accattcact gggtggattc gggtgtggat acaggccagg
    13861 tcatcaaaca ggttcgtgtg ccacgactag ctgatgatac cattgacaga tttgaagctc
    13921 gcatccatga agcagagtac aggttgtatc cggaagtagt gaaggctcta tttacagatt
    13981 gactttttga tgattcatat gatatctttg attttaaatt ggagtcagtg tttgttgaag
    14041 acggcttcaa acggaggtat ttgtaatgtt agaatctaaa aaaacaactc gatatgtatt
    14101 ttatgtctat ctgatgttat taacttgggg aatcttattt aagtttgaaa caaatcctga
    14161 atttatagca tttttcttag ctccaaggta tatcaattgg attccatttt cagaaccact
    14221 aatagtcgat ggaaaaattg tttttgctga aatgttattt aatctgattt cctttattcc
    14281 attaggtgtt tgtttccctt tgataaaaac taatttatct agtttaagaa tagtcgggac
    14341 aggtttcttg attagtttat tgtttgagtg cttacagtat attttagcaa taggtataac
    14401 agatataacg gatttgactt taaatacgct aggtgtctgt gtaggcttac tgatttatca
    14461 aatttttata agagtgttca aatcacagac tagaaaatgg atcaatatct taggtatgct
    14521 tagccttggt tttgcttatc ttgttttact gttactgcat ttaattggtg tttaactaat
    14581 gattaaaaag gagaatataa tgactaaacg cgtcttaatc agcgtctcag acaaagcggg
    14641 cattgttgaa tttgcccaag aactcaaaaa acttggttgg gagattatct caacaggtgg
    14701 aactaaggtt gcccttgata atgctggggt ggataccatt gctatcgatg atgtgactgg
    14761 tttcccagaa atgatggacg gtcgtgtgaa gaccctccac ccaaatatcc acggagggct
    14821 tctcgctcgt cgtgacttgg atagccactt ggaagcggct aaggacaaca agattgagct
    14881 cattgacctt gtggtggtca acctttaccc atttaaggaa actatcctta aaccagatgt
    14941 gacttatgct gatgcagttg aaaatatcga tattggtggg ccgtctatgc ttcgttcagc
    15001 agcgaaaaat catgccagtg ttacagttgt ggtagatcct gctgactacg ctgtggtttt
    15061 ggacgaattg gcagcaaacg gcgaaacctc ttatgaaact cgccaacgtt tagcagccaa
    15121 agtatttcgt cacacagcgg cttatgacgc cttgattgca gaatacttca cagctcaagt
    15181 gggtgaaagc aagcctgaaa aactcacttt gacttatgac ctcaagcaac caatgcgtta
    15241 cggtgagaat cctcaacaag acgcggactt ttaccagaaa gctttgccta cagattactc
    15301 tattgcttca gccaaacagc tcaacgggaa agaattgtca tttaataata tccgtgatgc
    15361 agatgctgct atccgtatca tccgtgactt caaagatagt ccaaccgttg tggctctcaa
    15421 acacatgaat ccatgtggaa ttggtcaagc tgatgacatc gagactgctt gggactacgc
    15481 ttatgagtct gacccagtat ctatctttgg tgggattgtc gtcctcaacc gtgaggtgga
    15541 tgctgcgaca gctgagaaga tgcacggcgt tttcctcgaa atcatcattg caccaagcta
    15601 tacggatgaa gcgctagcta ttttgatcaa taaaaagaaa aacttgcgta tccttgcctt
    15661 gccatttaat gctcaagagg ctagcgaagt ggaagcagaa tacacaggtg tagtcggtgg
    15721 acttctcgtg caaaatcaag acgtggtcaa ggaaagccca gctgactggc aagtggtgac
    15781 caaacgtcag ccaactgaga cagaagcgac tgctcttgag ttcgcttgga aggctatcaa
    15841 gtacgtcaaa tcaaatggta ttatcgtgac caacgaccac atgacacttg gtgttggtcc
    15901 aggtcaaacc aaccgtgtgg cttctgttcg ccttgccatt gaccaagcca aagatcgtct
    15961 gaacggggcg gtccttgctt cagatgcctt cttcccattt gcggataacg tggaagaaat
    16021 cgccaaagca ggaattaagg ccatcatcca gcccggtggc tctgtccgtg accaagaatt
    16081 catcgaagca gcggataaat acggcttgac tatggtcttt acaggtgtga gacattttag
    16141 acattaagaa gataaaaggg aagaaaacag tttctttcct tttttggctt aaaatgctaa
    16201 ctgaaacaag attaaaacga acttttttga tataatgttg gtaaataatt cgcaaaagag
    16261 gttgaggaat gaaactgctt gttgtcggtt ctggtggtcg tgagcatgcg attgctaaaa
    16321 agttacttga atcaaaagac gtggaaaaag tctttgtagc tcctgggaat gacggtatga
    16381 ctctggatgg tctggaattg gtaaatatat ctatttccga acattataaa ttgattgact
    16441 tcgcaaaggc caacgatatt gcttggacct ttatcggtcc agatgacgcc cttgcggctg
    16501 gtatcgtgga tgatttccat gcagctggtc tcaaagcctt tggtccgaca agattggcag
    16561 cggagctgga gtggtccaag gatttcgcca aggaaatcat ggtcaaatat ggtgtttcga
    16621 cagcagccta tggcacattt tcagatttcg aggaagcaaa ggcctatatc gaaaagcatg
    16681 gtgcgcctat cgtagtcaag gcggatggct tggcgcttgg gaagggtgtc gtcgttgctg
    16741 agacggttga gcaagcggtc gaagccgctc atgagatgct tttggacaat aaatttggtg
    16801 actcaggtgc gcgcgtggtt attgaggaat tccttgaagg agaggaattt tcactctttg
    16861 cctttgtcaa tggtgataag ttctacatca tgccaacggc tcaggaccac aaacgtgcct
    16921 acgatggcga caaagggcct aacacgggtg gtatgggtgc ctatgcgcca gttccacact
    16981 taccacagag tgtagttgat acagcggttg acaccattgt caagccagtt ctagaaggga
    17041 tgattaaaga aggtcgccct tatctgggag ttctttacgc agggcttatc ctgacagctg
    17101 atggaccgaa agtcattgag ttcaacgctc ggttcggaga tccagaaact cagattatct
    17161 tgcctcgctt gacctctgac tttgctcaaa atatcacaga tatcctggat agcaaggagc
    17221 caaatatcat gtggacggac aagggtgtga ctctgggtgt ggttgtcgca tccaagggct
    17281 acccgctaga ctatgaaagg ggcgttgagt tgccagccaa gacagaaggc gatgtcatca
    17341 cctactatgc aggggctaag tttgcggaaa atagcagagc actgctctca aacggcggac
    17401 gagtttatat gctcgttacc acagcagata ccgtcaaaga agcccaagcc agcatctacc
    17461 aagaactatc ccaacaaaaa atagaaggac tcttctaccg aacagatatc ggaagcaagg
    17521 caattaagta aagatataag aataacgcgc cgtagtcgcc aaacacgata atggtcgtcg
    17581 tggtgaaaag accagaacag tgaatgttct ggtcaggggg aaacttggag accgtaggct
    17641 caaagtttag gaatgaaacc gaaggtttgc ttccgcctcc atcacctaag accattatca
    17701 aaaagaaaaa taaaaattca caaaatacgt taatgatcgt atggtttgcg agcgttagcg
    17761 agctaatata gaacaatcac cgccgttgtg aaagaacgat tggatgataa tccaatcgtt
    17821 cagggaaatt ggaagacctt gggtttccaa tttaggcatg agacaccttt ggtggctgct
    17881 gccgtccctc acaagctaag gtgattgttg aaaaagagga aaaaggagaa gaaatgaaac
    17941 cagtaatttc catcatcatg ggctcaaaat ccgactgggc aaccatgcaa aaaacagcag
    18001 aagtcctaga ccgcttcggt gtagcctacg aaaagaaagt tgtttccgca caccgtacac
    18061 cagacctcat gttcaaacat gcagaagaag cccgtagtcg tggcatcaag atcatcatcg
    18121 caggtgctgg tggcgcagcg catttgccag gcatggtagc tgccaaaaca acccttccag
    18181 tcattggtgt gccagtcaag tctcgtgctc ttagtggagt ggattcactc tattctatcg
    18241 ttcagatgcc gggtggggtg cctgttgcga ccatggctat cggtgaagct ggagcgacta
    18301 acgcagctct ctttgccctc cgtctcctct ctgtagaaga taagtccatt gcggatgcac
    18361 ttgccaactt tgctgaagaa caaggaaaaa tcgcagagga gtcgtcaaat gagctcatct
    18421 aaaacaatcg gaattatcgg tggcggtcaa ctgggtcaga tgatggccat ttctgctatc
    18481 tacatgggcc acaaggttat cgcgctggat cctgcggcgg attgcccggc ctctcgcgtg
    18541 gcggaaatca ttgtggcacc ttataacgat gtggacgccc tccgtcagtt ggcagaccgt
    18601 tgcgatgtcc tcacttatga gtttgaaaat gtcgacgctg acggtttgga tgccgttatc
    18661 aaggatggac aactccctca aggaacagat ctgctccgca tttcgcaaaa tcgtattttt
    18721 gaaaaggact ttttgtcaaa caaggctcaa gtcactgtgg caccctacaa ggtcgtgact
    18781 tctagcctag acttggcaga tatcgacttg tcgaaaaact atgtcctcaa gactgcgact
    18841 ggtggctacg atggtcatgg acaaaaggtt attcgttcag aagcagactt ggaagcagcc
    18901 tatgcgctag cagactcagc agactgcgtc ttggaagaat ttgtcaactt tgaccttgag
    18961 atttctgtca tcgtgtcagg aaatggcaag gaggtgacgt ttttcccagt tcaggaaaat
    19021 atccaccgca acaatatcct gtctaagacc atcgtaccag cccgcatttc tgaaagtcta
    19081 gtagacaagg ctaaagctat ggcagtgcga atcgcagaac aactcaactt gtctggaact
    19141 ctctgtgtgg aaatgtttgc gactgatgat gacgttattg tcaatgaaat cgccccacga
    19201 ccacataact ctgggcacta ttctattgaa gcctgtgatt tctctcagtt tgacacccat
    19261 attctcggtg ttctcggagc accattacca gtcatcaaac tccatgcgcc tgccgtcatg
    19321 cttaacgttc tcggccagca tgttgaggcc gctgaaaaat atgtcacaga aaattcaagc
    19381 gcccacctcc acatgtatgg taaaatagaa gcgaagcaca accgcaagat gggacatgtg
    19441 actttgatta gtaatgaacc agatagtgtg ggagagtttt aggggagagg ggtctaaaga
    19501 aaaaatgttt gaaaaaatta aaggaattaa tataaaatca ggtatttttg aagatgaaac
    19561 aaaattagaa ctctttgaag gaaattttga gggaacaaat ccagtacaaa acgatagggc
    19621 aagtttattg tttggaagga atggttcagg gaaaagtacc attgctcgag gaataaatca
    19681 attaaaaaat ggtgagattg gaacagatag agtatcattt attgataaaa ataataataa
    19741 tattgtactt agtgatactg aacgaaaatc gatatttgtt ttcaatgaac actatgttga
    19801 tcagaaagtc aaaattgctc aagaagggtt agatacaatt gtaattttag gtgaacaggt
    19861 tgatatcgat gaggaactgg atagattaag aacgcagtta tcagaatcac aaattgaatc
    19921 tcaagattat tatgctgaat atgaagaata tttagatgaa aaaaatgaaa aatctccaga
    19981 tttttggaaa aaggaaatga cagattcttt gaaaggagta ggcaactggg ccgaaagaga
    20041 tagggagata aaaggaaatc gagctgcatc tcccgttcat aataatacct ttcaaaattt
    20101 tgttgattta caaccaatct tagataaaaa tgagttagag gttgaattca acaataaaaa
    20161 agctagatat ttttcaataa gagattccgc agttactata aataatgaac tatctttacc
    20221 tgatattaat tttgatagca atgaattatc aactttattg tctgaaaaaa tcgaagaacc
    20281 tgagttgaat tctagggata agtatttact gacattatta tctgattcca caaaaggtga
    20341 acgtcatttg agagaagtta aagatttctt tgaagatgaa catcaaaaaa aatgtccttt
    20401 ctgtactcaa tcagtatcag aagatgttaa agttgaactc acgaatggca taactaaact
    20461 actatcaaga gcagtcgaag aacaccaatc cgcattgaga ggtaaaaaaa ttgatgagat
    20521 taatcaagat ttttctggtt atgaacagat agatccaata ttaattcagt cttatcaaaa
    20581 tagtattaat gctttgaatg ctaaatttaa cgaaattaac tcaataatcg ataagaagat
    20641 tgataatcca tataatatag ttgagttacc taatataagc ttttctcaag aattaagtca
    20701 agcagagcat gatattgaaa aaattaacca agcaattatc aaacataatt cagaaatctc
    20761 gggtattcaa aagttaaaag tagatctcct tcaaataaat aatgaattag ctttttatga
    20821 aattcaagat gcatacaaaa agtttcaaga gaaaacaaat aaaaaagcaa tttgtgaaaa
    20881 taattataat aacagtagta aacgagtaaa ggattatgag aagcagattt ctgatttaga
    20941 agacaagaaa cttaatatag atattgcagt tgatgaaatt aataaaagtt taaattacat
    21001 atttttttca aaaaatcggt tagctataca gaatcaaaac ggaaaatact atctgttatc
    21061 aagaggtaag tctgtggtcc ccagtagggt ttccgtaggg gaacgaaatg cgctggcatt
    21121 atgttatttc tttactgaga taattcaaca gagagagtta gcagatgctt atagtcatga
    21181 atattttatt gttattgacg atccaatttc tagttttgat atggaaaata aagttggaat
    21241 aacctcgtat ttaaaatatt gtttaactag attttttaag gggaatagta atactagagt
    21301 tttactaatg acccatgata aacaaacaat atatgacttt gatatctttt tgaaagagat
    21361 aatggaatcc tgtaaagaag aggaaggtgg acaaaaatct aaatacaaga agttagaatt
    21421 ggtttctggt aaattacaag aattcaaaac tagtactcac gattatacag aactacttga
    21481 aatagtcttc ggttatgcat tgggaaatag tacacctact tcggaaagtt ttgttgggaa
    21541 tgcaatgaga aaaattttag aagcctatgg tagttttaat tataagaagg gaattgccga
    21601 gctaacaaca gatcctttaa ttgttgaaaa aattgataaa gaatacagaa cttattttga
    21661 gaatcttatg tatagactgg ttttaaacgg tgaaagtcat tttaaggatc cggttaaaac
    21721 tttgagtatt gatttctttg ataccatttc tgatgaagaa cgaaaaaaaa cagctagaga
    21781 tttactagtt ttgctttatc ttttagatga tttacatgtt ttaaaacatt tagagggtgt
    21841 atcaaatgcg gaaaatagac ttgaacaatg gaagtgtgag attttagaat aagagaatca
    21901 taccatttga cttattgcaa tggttgaagt ttgcatgata gctgattaga gtaatcaaag
    21961 gattattact tcctaatttt attttctaca aaccctaatt tagcatctaa taatgcaaag
    22021 tttcctgagc ctcatagata aaaaagattt ttaaggagaa aaacatgatc aaccgttact
    22081 ctcgccctga gatggcgaat atttggagtg aagaaaataa ataccgtgct tggcttgagg
    22141 tggaaatcct ctctgacgag gcatgggctg agttggggga aatccctaag gaagatgtgg
    22201 ctttgattcg caagaaggcg gactttgaca tcgaccgtat tttggaaatt gagcaggaga
    22261 cgcgccacga tgtggtggct ttcacgcgtg cggtttctga gactcttggt gaagagcgca
    22321 agtgggttca ctatgggtta acttctaccg acgtggtgga tactgcctat ggttacctct
    22381 acaagcaggc caacgacatc atccgtcgtg accttgaaaa cttcactaat atcatcgctg
    22441 acaaggccaa ggagcacaag ttcaccatca tgatggggcg tactcatggt gtgcacgctg
    22501 agccgacaac ttttggtctt aaattggcaa cttggtatag cgaaatgaaa cgcaatatcg
    22561 agcgcttcga gcatgcggct gctggtgtag aagctggtaa gatttctggt gcggttggga
    22621 actttgccaa tatcccacca tttgtagagg agtatgtctg cgataaactt ggcatccgtg
    22681 cccaagaaat ctctacacaa gtccttcctc gtgaccttca cgctgagtac tttgcggttc
    22741 ttgccagcat tgcgacttca atcgaacgta tggcgactga gattcgtggt ctacaaaaat
    22801 ctgagcaacg cgaagtagaa gagttctttg ctaaagggca aaaagggtct tcagcaatgc
    22861 ctcacaaacg caacccaatc ggttctgaaa atatgactgg tctggcgcgt gtcattcgtg
    22921 gtcacatgat tacggcttat gaaaacgtcg ctctctggca cgaacgcgat atttctcact
    22981 catcagctga gcgtatcatc acaccagata cgaccatttt gattgactac atgctcaacc
    23041 gttttggaaa tatcgtcaag aacttgacag tcttcccaga aaatatgatc cgaaacatga
    23101 actcgacttt tggtcttatc tttagccaac gggctatgtt gacattgatt gaaaaaggca
    23161 tgacccgtga gcaagcctat gacttggtgc aaccaaaaac agcctactct tgggacaacc
    23221 aagtagactt taaaccactt cttgaggcag attcagaagt aacatcacgt ctcacacaag
    23281 aagaaatcaa tgaaatcttc aacccaattt attacaccaa acgagtggat gatatctttg
    23341 aacgtcttgg actaggtgat taattaaaaa ataaacagcg agcttcaatc tcgctgttta
    23401 ttttttatcg aaaagactta gtcttctttt cttttagtga gtccataggc tgctagtgtg
    23461 gacatgagtc ctgcgactac tagtcctgca gaatcgtgag ttcctgtttc aggaagtttt
    23521 ttctctgtta ccacaggagc tggatcttga ggaagagctt tgctttcctc agcaggagca
    23581 gttgatggag ctggttggct tgggatttct agtcttggtt tttcttcagc aatagcggct
    23641 tgtccgtttt catcgcctac atgtgttacc atagttccga cttcgactat ttgagtaacg
    23701 gcttcctgtg ctacgacact atttacaagt gttttcactt ccttaccatc ggcagaagtg
    23761 ctcacagagt agaagttgct acgatgtcca ttgacgccct tagtaatgac ttgtgttttt
    23821 cctttgagta agagtggatt ttcacgagtc actgtggtaa atggaatttc ttctttttgg
    23881 atatccagtc taggttttac ctcagtagtt ggtgcaagac cactttcatc acccttgtga
    23941 gttacaggag cgccaacttc aaccacttgg tttataactt ctttggttac ctggctatca
    24001 aggactgttt ctgttgtttt tccattttca gtgagtacag agatgtaatg agttcgttca
    24061 cctttgactc ctgctgtgat aatattttcc tgaccagctg ggaggttagg attttctttc
    24121 ttgataactt caaatggaat ttcttcagtt cttgtgatga gttctggtct ggtttcaaca
    24181 ttggcagcca cttcattttc atccaggctt cctgaatgag ttgcagctgg tttgaggcct
    24241 tgaagagcgg cttttaggtt ggctacaagt gtgtcaagct cagcttgttt attacggttg
    24301 aggttgtaat ttagagctgt tttagctgcg tcaagggcct caagactttc tttactatat
    24361 ccttctaagt ttgtaggaat tttagctaat tcttcgcgga gagcattata attagcacgg
    24421 aagtagtctt tgttgtggtc tgcaaaggca gtcatgagtt caaagatttc ctcttccttg
    24481 tactcagcgc ttggtctatc tgcccagatt gaaagcatac ttccgactgt tggaagatct
    24541 acttcaggat atttggtaga agctagttga ttgaatggaa ctttaccaga attttcaact
    24601 gcttttgaaa gagggtaagc ttcatcttgt ttgtgattgc ctataacata ataccagtca
    24661 ccgttggtat tcaagaattt atagcctttg cttgctaggt attgaggtga tgcgaggtta
    24721 tatccccacc agcctttaga ccagtaagaa atcaagacat ctttgtcaaa ctgaacatcg
    24781 tccttgtctt catagtagaa gccatcgttg aaggccattg gttgaagccc tctttctttg
    24841 gccatagctg cgagggtgtt ggcatattcg gcaaatttgc catagagttg ataccacttg
    24901 aggtagtacc agccttgggc actagtcgca tcgttggcgt attcgtcagt accaaagttg
    24961 aaaatctttg ttttacctgc aaagaagtcc atgtatttac cgatgagggc ttttacaaag
    25021 ttcatcgctt cttcgttttt caagtccata gttgtttttg aaactttatc aaagtgggct
    25081 tgaggatttt taatacctaa tttttccatg gcaaccagca tagcatccat gtgacctgga
    25141 ctgttaatag ctgggatgag accaatgtcc ttagatttag cgtattcaat tagctctgtc
    25201 acttctgcct gtgttagtgc agtaccgttt ggatcgtcgt agtaagcttt agttccttcg
    25261 ataatagctt ttttaacgtc atcactagca taggtttttc cgttggcagt aatggtcata
    25321 tcattgagta gaaagcgaag tccgtcattt cctagaagga gatggacatc agaatatccg
    25381 agctcactgg ccttgtctac gatgcgtttg agctggtcca gagtaaagta tttgcgtcca
    25441 gcatcgattg agattacctt gtttttggca agtttttcaa cctcacgttt agcttcttct
    25501 tctttttgag cttcaggcgt gagggtcaag ttgttgacag tttcttgaag tttagcaatg
    25561 gcttgatcaa tcgtatcttg ttgggcacgg ctaaggttgc tatcgagaga gcgaatagct
    25621 ttttcagctt cttttacggc cgtgacgctt tctgcagtat aacggttcag gtcttttggt
    25681 acctcgttaa gtgcttgctc tgcagattca taatcagctg cgaagtattc agcgttggca
    25741 tttgcaaaat gacgcatgag tttgaagagg cgtgatggtg aataacgtgc agatggagtg
    25801 tcagcccaag cagctaccat accaccgatg attgggatat cagctccttc tgtttttggt
    25861 acagaagtga ttggtgtgtt tttaatacca ttgagcccct gatcgagatt gtaccagcct
    25921 tggccatcag cgtttcgtcc aagaacgtag taccaagcat cattggtatt aaggatttgg
    25981 tgaccttttt cagctagtag tttagaagaa gcgacatcgt agcctcccca accaccagtc
    26041 cacatagaaa cgatgatgtc tttgtcaaaa ctaccaaagc ttgtgtcgct attgtagtag
    26101 ataccgtcgt taaaggccat tggtttgaga ccgtgcgatt ttacaatacg agcgaggtca
    26161 ttggcgtagg caataaattt ttcatagcct tttacagggt agccttcgtt tggatagtat
    26221 ttatcagctt gaagcacact ccaaccttta gcatctgtcg catcattggc atattcatca
    26281 agtccgatgt tgaagatttc agtctttttc gcgaaataag cagcatactt gtcgataagg
    26341 gcttttgtaa aagcgacagc ttgttcgttg tcaagatcga cagtacgggc tgatttcttc
    26401 ccaaaatagc taaagttagg gttttggatt cccaattctt tcatggcatt gagaatcgca
    26461 tccatgtgtc caggactatt tactgtcgga atgagaccga tacctttatc tttggcatag
    26521 ttaatcagat ctgtcatttg actttctgtt aagtgattgc cgtttggatc gttgtaataa
    26581 tcatttgtac ctttttcaat ggcgcgtttg acatcgtcac tggcataggt cttgccgtta
    26641 gctgtgatgc tcatatcgtc caacatgaaa cggagtccat catttccgac taataggtgt
    26701 aaatcagtgt agccataatg tttcgcttta tcgatgattt ccttgagctg ttctggtgag
    26761 aaatatttac gtccagcatc aatagaaaca attttctttt tcgctagttt ttcatttaca
    26821 gttgcagcac gttcctttcc tgtctctgtt tccggtttgt cagcctctgc tttcgcttca
    26881 tcttttttag ctggtttatc cttgtcagtc ttgtctgtat ttgactcttt agaatcaacc
    26941 tctttcgctt cttccttttt agggctagct tcttcttcct ttttattagc agtttctttt
    27001 tcagcagaag ttggagttac cacctctgct ttatcactag gagttgaact aacttcctct
    27061 tgtggttttt cttctgtttt tggaagacta gctaccttat cagtagctgg agtttctgtt
    27121 tctacagttt ttggagcttc tggttgaagc tctgctttag gtgtttcctc agtccgattt
    27181 tcggatgatt gaggggaatc agaaaccgta tggatggtcg gttggttttc tgtagtagta
    27241 ggagtaactc catcggctgc aacagtctgt gcttggaagg caaatccaat tagaacagaa
    27301 gctgctccta cagcgtattt acgaatagaa aaacgctgtt gtttttcatg tttcattgca
    27361 aaacctcctg attgcattgt tatattgata gcgattatat aaatcaacgc ctttatttta
    27421 tttcttatat taacgagagt caagaggaga tgacaaaaaa ctataataag tataaaaaaa
    27481 tataaaattt aaacttaaga tttcagattg gtcgaaaaaa tacatatata tatctagtat
    27541 aatttttggt tctatttcta taaaatattc cacaaattat agaatcttcc aaaaataggt
    27601 aagcgctacc tttttggtgt ggtataataa gcatagaaaa agcccgagcg attaggctca
    27661 ggctttcttc ttagtgatca cggtcacatg agatgaattt aatcttgtag taatcagatc
    27721 gtttgtaagt ttcactgtat tctaaaactt ggccagttga ttcgagtttg gtgattttag
    27781 tttgtaggac agtagggaat tgttcatcga ctccgaggac tgaagccgca tgttctggag
    27841 ttggaaagac tatttcgttg atttcttcaa agtgttcatc attcatgtga atgtggtagt
    27901 ctaacttgaa acgattatag atagaactat agtattcaag gtttggataa tttgcgttga
    27961 tatattgttc tgggatgtag gatgtatggt agatataaac gacaccgttt gattcgcgga
    28021 tacgttcaat cttgtagtag aattgatcgc cgcgtagacc caatttttcc aagtaaacaa
    28081 gcttgtttcc gcgttcaatt gaaagaacag ttaccttatc atctttagca ttgaagagtt
    28141 caatatctga aaactctaca agcttgtgtt tgcgtgcacg tgaaacgaag gttccttttc
    28201 cttgttggcg gacaatatag ccatctttgg caaggtcgtt taaggcgcga acaactgtga
    28261 tagagctgac atcgtacatt gaaatgagtt ctgcttcagt gtaaaattta tctccactgc
    28321 taaactgccc agagatgatt ttatttttta attcgtcttt tatgtattga tatttgggaa
    28381 ttgccatatt tttacctcta ttttccttct ccatagaaaa ttttaccata aaagcgaaaa
    28441 aaatagtagt cttttgaata aaaaattaga ataatagttt gaaaaattaa gcgatatagg
    28501 gattgctcat attttatcaa tgcattctat acaaaattat tttaggtcct atattccgaa
    28561 tttaaagagc gtttgaaatg ggtctaaaaa tgtttgaaac taattgaatg tgtgtataat
    28621 agtaaaaatg cagtaaatat cccgacaatt tctattgaca attcaaacag attggtttat
    28681 aattaatata acaacaaatg aaagcgcaaa ctttcgcggt cggaaggtag ttttatgaca
    28741 cgatttgaga tacgagatga tttctatctc gatggaaaat catttaagat tttatctggt
    28801 gccattcatt attttaggat tcctccagag gattggtatc attcgctcta taacttgaag
    28861 gctcttggtt ttaatacggt agagacttat gttgcttgga atttacacga gcctcgtgaa
    28921 ggtgagtttc attttgaagg tgatctggat ttagagaaat ttctccaaat agcgcaggat
    28981 ttgggtctct acgcaattgt gcgtccgtct ccatttatct gtgcggaatg ggaattcggt
    29041 ggcttaccag cttggctctt gaccaagaac atgcgaattc gctcatccga cccagcatat
    29101 atcgaggcag ttggtcgcta ctatgatcag ttattgccaa gactggtgcc tcgtttgttg
    29161 aacaatggtg gcaatattct catgatgcag gttgaaaatg agtatggttc ttacggagaa
    29221 gataaggctt acctgagagc gattcgacag ctaatggaag agtgtggcgt aacctgtccc
    29281 ctctttacat cagatggtcc atggcgagct actctgaaag ctggaacctt aattgaagag
    29341 gacctctttg taacaggaaa ctttggttct aaggcacctt acaacttttc gcagatgcag
    29401 gaattctttg atgaacatgg taagaaatgg ccactcatgt gtatggagtt ctgggatggt
    29461 tggttcaatc gctggaaaga accgattatc acacgggatc ctaaggaatt ggcagatgca
    29521 gttcgagagg ttttggaaca aggctctatc aatctttaca tgttccacgg tggtacaaac
    29581 tttggtttca tgaatggttg ctcagctcga ggaactttgg acctgccaca agttacatct
    29641 tatgattacg atgcccttct ggatgaagaa ggaaatccaa ctgctaaata tcttgcagtc
    29701 aagaagatga tggcaacaca tttttcagag tatccgcagt tggaaccact ctacaaagag
    29761 agtatggagt tggatgctat tccactagtt gaaaaagttt ctttgtttga aaccttagat
    29821 agcttgtcaa gtcctgtaga aagtctctat cctcaaaaga tggaggagct gggacaaagt
    29881 tatggctacc tactttatcg aacagaaaca aactgggatg cagaagaaga aagacttcgt
    29941 atcattgatg gtcgagatag ggcccagctg tatgtcgatg gtcagtgggt taaaactcaa
    30001 tatcagacag agattgggga agatattttt tatcaaggta aaaagaaagg gctatctagg
    30061 ttagatatct tgatagaaaa tatggggcgt gtcaactatg ggcataagtt cttagcggat
    30121 acgcaacgta agggaattcg gacaggggtc tgtaaggatc tgcatttctt actaaactgg
    30181 aaacactatc cactcccact agacaatcct gagaaaattg atttttcaaa aggatggact
    30241 caaggacaac cagcctttta cgcttatgac tttacagtcg aagagccaaa agatacttac
    30301 ctagacttgt ctgagtttgg taagggagtt gcctttgtca atgggcagaa tctaggacgt
    30361 ttttggaacg ttggcccaac tctctcactt tatatccctc atagctatct caaggaaggt
    30421 gccaaccgta tcattatctt tgaaacagaa ggtcaatata aagaagagat tcatttaact
    30481 cgtaaaccta cactaaaaca tataaagggg gaaaacttat gacaattgta ggatgccgta
    30541 ttgatggacg tttgatccac ggacaagtag ccaatctttg ggctggaaaa ctaaatgttt
    30601 cacgcattat ggttgtagac gacgaagttg tcaacaacga tattgaaaag agtggtttga
    30661 aacttgcgac accaccaggt gtgaaattga gtattttgcc agttgagaaa gctgcagcca
    30721 atattcttgc tggtaaatac gatagccaac gtctctttat cgtggctcgt aaaccagacc
    30781 gcttccttgg tttggtagaa gcaggtgtac cacttgaaac ccttaatgtt gggaatatgt
    30841 ctcaaacacc agaaactcgt tctattacac gttctatcaa cgtagtagac aaggatgtgg
    30901 aagacttcca caaactggca gaaaaaggtg ttaaacttac tgctcagatg gttccaaatg
    30961 atccaatttc agactttttg agcttattaa aataggaaaa aaatttttag gaggtcattg
    31021 ttatgataca atggtggcaa attttacttc tcactttgta ctcagcttat caaatctgtg
    31081 atgagttgac gatcgtttca tctgcaggtt cccctgtatt tgctggtttc attactggtt
    31141 taatcatggg agatgtgact actggtttac ttatcggtgg taacttgcaa ctgttcgttc
    31201 ttggggttgg taccttcggt ggtgcttctc gtatcgacgc aacttctggt gcggttcttg
    31261 cgacagcctt ctctgtttca caaggaattg atgcaccgct tgccattact acaatcgctg
    31321 taccagtagc agctctcttg acttacttcg acgttcttgg tcgtatgact actaccttct
    31381 tcgctcaccg tgtggatgct gcaatcgaac gctttgacta taaaggtatt gaacgcaact
    31441 acttgcttgg tgcgattccg tgggctctat ctcgtgccct tccagtcttc tttgcccttg
    31501 cttttggtgg tgcctttgta caatcagtag tagacttcgt tgaagcctac aaatgggttg
    31561 cagatggctt gacacttgca ggacgtatgc ttccaggtct tggatttgca atcttgcttc
    31621 gttaccttcc agttaaacgt aaccttcact accttgctat gggatttggt ttgacagcta
    31681 tgttgactgt tctttactca tatgtaacag gtcttggtgg cgctgttgct ggtatcgtag
    31741 gtactcttcc tgctgaagtt gctgaaaaaa ttggtttcgt gaacaacttc aaaggtttgt
    31801 ctatgattgg tatttctatc gtaggtattt tccttgcagt gcttcacttc aaaaatagcc
    31861 aaaaagtagc tgtagcagca ccttctacac catcagaaag tggggaaatc gaagatgacg
    31921 aattctaatt acaaacttac aaaagaagat tttaatcaaa tcaacaaacg tagcttgttt
    31981 actttccaat taggttggaa ctacgaacgt atgcaagctt ctggttacct ttacatgatc
    32041 ttgcctcagt tgcgtaaaat gtatggtgat ggaactcctg aattgaaaga aatgatgaaa
    32101 gttcatactc aattcttcaa tacttcacca ttcttccata ccattatcgc tggttttgac
    32161 cttgccatgg aagaaaaaga tggtgtaggt tcaaaagacg ctgttaacgg tatcaagaca
    32221 ggtttgatgg gaccattcgc tcctcttggg gatacaatct ttggttcact tgtacctgct
    32281 atcatggggt cagtcgcagc aactatggct atcgctggcc aaccttgggg gatcttcctt
    32341 tggattgcag ttgcagtagc gtatgacatc ttccgttgga aacagttgga atttgcttac
    32401 aaagaagggg ttaaccttat caacaacatg caaagtacct tgacagcttt gattgacgct
    32461 gcatctgtac ttggtgtctt catgatgggt gctcttgtag caacagtgat taactttgaa
    32521 atttcttaca agttgccaat cggtgaaaag atgattgatt tccaagacat cttgaaccaa
    32581 atcttcccac gtttgcttcc agcaatcttt actgccttta tcttctggtt gcttggtaag
    32641 aaaggtatga actctactaa agctatcggt attattatcg tacttgcttt gactctttct
    32701 gcccttggtc actttgcact tggaatgtaa ttccttatga ctaaatcatt aattttggtg
    32761 agccatggtc gcttctgtga ggagcttaga ggtagcacag aaatgattat gggcccacaa
    32821 gacaacattt acacagtagc tcttcttcca gaagatggcc cagaagaatt tactgctaaa
    32881 tttgaagctg ttattggagg attggatgat ttcctagtct ttgcggatct tctcggtggg
    32941 acaccttgta atgtggtgag tcgcttgatc atggaaggtc gtgatattga cctttacgca
    33001 gggatgaatc ttccaatggt gattgaattt atcaatgcga gccttacagg cgcagatgcg
    33061 gactacaaga gccgtgctgc agaaagcatt gtgaaagtta atgacctgtt agcgggcttc
    33121 gatgatgacg aagatgaata atactcttcg aaaatctctt caaactacgt caacgtcgcc
    33181 ttgccgtagg tatatgttac tgacttcgtc agtcttatcc ggcaacctca aaacggtgtt
    33241 ttgagctgac ttcgtcagtt ctatctgcaa cctcaaagta gtgctttgag cagcctgcgg
    33301 ctagtttcct acagatttta gttggaactc gattcaattc atgtgacaac gtgaaaatcg
    33361 ttagagcatt ttatatagaa tatacatggg aatggagctt actcccattc ccatatttaa
    33421 tagaaaaaga ggaactcaat gctacattat acaaaagaag acttgctcga attgggtgca
    33481 gaaatcacta cgcgtgaaat ctaccaacaa cctgatgtat ggagagaagc ttttgaattt
    33541 tatcaagcaa aacgtgaaga aattgcagcc ttcctacaag aaatcgctga taaacatgac
    33601 tatattaagg ttatcttgac aggtgctggg acttctgctt atgtgggaga caccttgcta
    33661 ccttacttta aggaagtcta tgacgaacgc aaatggaatt tcaatgctat tgcgacaaca
    33721 gatatcgttg ccaatccagc aacctatttg aaaaaagatg tggcaactgt ccttgtgtct
    33781 tttgctcgta gtgggaactc gcctgaaagt gtggcgactg ttgatttggc caaatccttg
    33841 gtggatgagc tttatcaagt gacgattact tgtgcagcag atggtaaatt ggctcttcaa
    33901 gctcacggtg atgaccgtaa tctcttgctc ttgcaaccag ctgtctctaa tgatgctgga
    33961 tttgccatga cttctagctt tacgtctatg atgttgacag ctctcttggt ctttgatcct
    34021 acagaatttg ctgttaagtc tgaacgtttt gaagttgtat ctagtcttgc ccgtaaagtt
    34081 ttagacaagg cagaagatgt caaaaagctc gttgatttag actttaaccg tgtcatctat
    34141 ctaggcgctg gtcctttctt tggacttgct catgaagctc agctcaagat tttggaatta
    34201 actgctggtc aagttgcgac catgtatgaa agcccagttg gcttccgtca cggtccaaaa
    34261 tctcttatca acgacaatac agttgttttg gtctttggta caacgacaga ctacactcgt
    34321 aagtacgact tggacttggt tcgtgaagtt gctggtgacc agattgctcg tcgtgttgtg
    34381 cttttgagtg atcaagcttt tggtcttgaa aatgtcaaag aagtggccct tgattgtggc
    34441 ggtgtcttga atgatattta ccgtgtcttc ccttacatcg tttatgccca actctttgcc
    34501 ctattgactt cactcaaggt agaaaataaa ccagatacac cgtctcctac aggtacagta
    34561 aaccgtgtag tacaaggtgt cataattcac gaatatcaaa agtaagacag tgtttatgaa
    34621 ttcttgacaa gaggatttgt aaattatcag ataaaccata gattgtcagt acgctttcta
    34681 tggtttgttt gcttgagaga aatagtaaaa ggagaacaga atgaaagcat acacagagcg
    34741 tgtatttgga aatgttgagg gcgaggatgt cttggcctat cgatttgaga cagacggtgg
    34801 ctaccaactt gaggttatga cttatggcgc gaccatcttg cgctatgtcg cacctgacaa
    34861 ggctggaaat tttgccaatg ttatcttggg atttgatgac tttgatagtt atgtaggcaa
    34921 tagtcccaag catggagcaa gtgtaggtcc tgtagcgggt cgtattgcag gtgcgacctt
    34981 tgagctcaat ggtaagacct atgaccttga ggttaataat gctagcaact gtaatcacag
    35041 tggttcaact ggttgggatt ccagcttgtt tgaagttgaa gaagtaagcg atcatggctt
    35101 gactctctac acagagcgta cagatgggac aggagggttc cctggaaatc tcaagatttg
    35161 gatcagttat cacttggaag aaactggtgc ctatgaaatc agctacaagg taacgaccga
    35221 tcaggatacg ctggtcaatc caaccaacca cagctatttc aacttgtctg gtgatttcac
    35281 gcagacgatt gaccgtcatg tcttccaact aaacacagag ggcatttact caatcgctcc
    35341 tgacggtgtt cctgccaaaa ctccagaagc caaccgtgat gtggtcaaac acatctacaa
    35401 tggtgccttg ttgaaggata tctttgcaga agaagatgag caaatccagc tggcatcagg
    35461 tttggatcat ccatttgccc ttcctgcagg ccatgacaat gctggattcc tttatgacca
    35521 aaattcaggt cgcttcctgc ttttcaagac agaagctcct tgctttgtgg tctacacagc
    35581 aaactttgtg gatgaaagtg tcatcatagg aggtcagcca atgctacagc acaatgggat
    35641 tgctcttgaa gcgcaagctt taccagatgc cattcacagt gaccttaaag gccaagtcat
    35701 tcttaaagct ggtcaaacct tcaccagtaa gacacgttat gaacttgttg tgaaataaaa
    35761 aagctggtca aaaagtcttt aaaattaaaa taacgcatat tgtcaggtga tgaaaagttt
    35821 gataatatgc gttttattat gaaatgattt actttatatt atcctgaaat ggtgttattt
    35881 ttgagttatc tggcttgact ttcttgatgg aaattatata atagttgcaa caataataat
    35941 tacaacgaaa aaagaggacc atttatgacc tatgaataca agagtcacat ttatttggca
    36001 gagacagttt taaatgtaaa ggatttggcc agtcaaacag ccttttatca gcaagttatc
    36061 ggtttagaaa tcttatctca aactgagaca gagtccattc taggccttgg tggaaaagtc
    36121 ttggtacagc tgattcaagc acaagagagc ggagaagtga gggaacatta tggtctttac
    36181 catttggcca ttctcttgcc cacacgcaag gctttggctg atgtcttgaa acacctgaca
    36241 gatttacaga ttcctcttgt cggcggtgca gaccatggtt acagtgaagc tatttacttg
    36301 gaagatttgg agggaaatgg cattgaactc tatcgagata agccagtttc cacatgggat
    36361 attcgagaag atggacgcat tatcggagtg acggaggctc ttgcggcgca ggacatttat
    36421 gagttggggg aaagagtaga gccctttatc ctggcagaag gtacgagaat ggggcatatt
    36481 catctttcag tcaaggatag tcgaaagtcc agccagtttt atcaaaaggt gttagggcta
    36541 gaggataaat tcagtgtgcc tagcgctagt tggattgcgg ctggagatta ccatcatcat
    36601 ttagcagtta acgaatgggg aggaaaaggt ctggcttcgc gtaagcaagg cttaccaggt
    36661 ttagcctact atgtcatcga agtcgcacat aaaaaagaac tgttaacgat tgcccaacga
    36721 gcacaagaag ttgacgcacc aatcaaatgg atgacatcga gccaattgga aatcacagac
    36781 tcagatggca tcgtgacccg tattcgttta gcaaggtaaa attaggaatt tttaataagt
    36841 tataagtatt gctagtttgc tttttgaacg gtctttggta acagaactgc gtgagctgga
    36901 tgggtgagaa ttttgacaaa gaagctgatt agagatataa tgaagaaaag tcgagcaagg
    36961 aaaaagaaat gacaaattca aagtatatta cttgtttgaa acgttcagag ggccagttgt
    37021 gtgggattca aaagatgatt gaaggagatc gtgactgtgc tgatcttgtg acgcaactga
    37081 cagcagtgag atctagtgta gagcgcgtga ttgagatgat aattaccgaa aatcttactg
    37141 aatgtatcaa tcagccacta gatgattctg aagctcaaaa ggaacgctta gaaaaggcta
    37201 tccgatactt gattaaacgg aaataaatga atgttttgag acgatatgtg caaaggtcta
    37261 ttaactatta gaatagttta atgtgagtca aaagaagttg taaaaaatct ttaaaattaa
    37321 aaaaacgcat agtatcaggt attgaaatgt actgccccca aaagttagat tttttctgtc
    37381 taacttttgg ggggcagtac ataaaaacct tgatagtatg cgttttattg tggggagatt
    37441 tatactcttc gaaaatctct tcaaaccacg tcagcttcac cttgccgtag atatggttac
    37501 tgacttcgtc agttctatcc acaaccttaa aacagtgttt tgagcagcct gcggctaact
    37561 tcctagtttg cactttaatt ttcattgagt attacttcat cttctcatga aattgagttt
    37621 ttgcccagct cctcttgatt attctacttg accgggctaa gcattcatac caccttctac
    37681 gttggtaacg gtgaggcctt gggagctgag aaattggcag gtagatgcag agcggactcc
    37741 tccttgacaa ataacatagt attcatggtc aggtttgagt tctttgtagc cttgctctaa
    37801 ggtacttaat ggaagatttt tggcacctgg tgcatgtcct gcttggaatt catgtgcttc
    37861 acggacatcg ataagttcta gattttcatt ttgatatttt ccataaaagt cagccatgct
    37921 gatactagtt tccatattct actccttctg ggttagccat tttgtatagt gaataagcgc
    37981 cgtcaaggtt ttggacggta aatcctgctt gcttgaggat aggctctgcg atatagctgc
    38041 gcaaactact gtggcaactg acgatgtagg cttgtttttc tcctcagttc atcagcttcc
    38101 tccttgacac tcggtcagat tttgatacaa tagtacaaaa ttagaggagg tgggctatga
    38161 ttcagaaaca tgcgattccc attttagagt ttgatgataa tcctcaggcg gttatcatgc
    38221 ccaatcacga ggggttggat ttgcagttgc caaagaagtg tgtctatgct tttttaggtg
    38281 aggagattga ccgctatgcg agggaagtag gggcgaactg tgttggcgaa ttcgtttctg
    38341 ctaccaagac ctatccagtc tatgtcatca actacaaggg tgaggaggtc tgtctggctc
    38401 aggctcctgt tggctccgct ccagcagccc agtttatgga ttggttgatt ggctatggtg
    38461 tggagcagat tatctctact gggacctgtg gtgtcctagc tgatatagag gaaaatgcct
    38521 ttctagtccc tgttcgcgct ttgcgagatg agggagccag ttaccactat gtggcacctt
    38581 gtcgttatat ggaaatgcag ccagaggcta ttgctgctat tgaggaagtt ttggaagaca
    38641 gagggattcc ttatgaagaa gtcatgacct ggacgacaga cggtttttac cgagaaacgg
    38701 ctgaaaaggt ggcttatcgt aaggaagaag gctgtgctgt tgtggagatg gagtgttctg
    38761 ctcttgctgc agtagctcaa ttgcgtgggg ttctctgggg tgaattgttg ttcacagcag
    38821 attctctagc ggacttggac cagtacgaca gtcgtgactg gggctcggaa gcttttaata
    38881 aggcgctaga actgagttta gcaagtgttc accaccttta gttgtactgg caaaggattt
    38941 gttttatcat aaaatgtcta gctcatactt ttcaaaaata tgtttaaacg aggtcacctt
    39001 cctcttgtcc taggcatgtt gaggttggga aaaatcttta aaatcagaaa aacgtatcat
    39061 atcaggtgat gaaaattttg atactatgcg ttttatgttg gtaagattta gagtgagatg
    39121 aaatgatact cttcgaaaat ctcttcaaac caggtcagct tcaccttgcc gtatagatgt
    39181 tactgacttc gtcagtt
//
LOCUS       DBEJAQ010000017        35941 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.017, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000017 DBEJAQ010000000
VERSION     DBEJAQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.017"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..933)
                     /locus_tag="ACL297_000911"
     CDS             complement(<1..933)
                     /locus_tag="ACL297_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HGR0071195.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEEKAKKAEEAAKEVDDASLA
                     VQKAYVEQRKAQEEKNKHSGNPEYAKKLAEADKKITEETTKLTDAQTKFQSVQAMVVP
                     EPEQLAETKKKAEEAKAEEKVAKRKYDYATLKVVLAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLL
                     DSLDPEGKTQDELDKEAEEAELDKKADELQNKVAD"
     gene            complement(1219..1758)
                     /locus_tag="ACL297_000912"
     CDS             complement(1219..1758)
                     /locus_tag="ACL297_000912"
                     /inference="COORDINATES: protein motif:HMM:NF037419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HGR0071196.1"
                     /translation="MLFYFEKIWSLSLKLLDCILDYQERFNRKACQVSTNYKYLETFK
                     VNFCLTDLHHLFGLHKITRDYASQTKPAIQAGVFILEDFRNILLYNDVIERISLYEFI
                     GDIFYFKITSCCIVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFT
                     SAKKYAKVRKTDVKEMKWL"
     gene            complement(2181..2378)
                     /locus_tag="ACL297_000913"
     CDS             complement(2181..2378)
                     /locus_tag="ACL297_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071197.1"
                     /translation="MKHFFAGIGEIKLVGYLLYICVGLAPLFHIYVIGSELSFVKIVL
                     SILGVIFVSMLTIARIYRTFL"
     gene            complement(2857..3585)
                     /locus_tag="ACL297_000914"
     CDS             complement(2857..3585)
                     /locus_tag="ACL297_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071198.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIVYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEFELFKNSK"
     gene            complement(3582..3914)
                     /locus_tag="ACL297_000915"
     CDS             complement(3582..3914)
                     /locus_tag="ACL297_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGR0071199.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            complement(3970..4872)
                     /locus_tag="ACL297_000916"
     CDS             complement(3970..4872)
                     /locus_tag="ACL297_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071200.1"
                     /translation="MMRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFVIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(4878..5195)
                     /locus_tag="ACL297_000917"
     CDS             complement(4878..5195)
                     /locus_tag="ACL297_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071201.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGDWNTFKNNIKGLARASSGFRF
                     AARETGLWAAIWSTRVYSYTYVVSAFAPILGSVAAVNAALAVIGGISVAGITYALWTR
                     KFY"
     gene            complement(5340..6692)
                     /locus_tag="ACL297_000918"
     CDS             complement(5340..6692)
                     /locus_tag="ACL297_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HGR0071202.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQALQNELLQLQQQGDSPSLSDTTSADNKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLVSEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLLPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(6689..7156)
                     /locus_tag="ACL297_000919"
     CDS             complement(6689..7156)
                     /locus_tag="ACL297_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGR0071203.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(7149..9368)
                     /locus_tag="ACL297_000920"
     CDS             complement(7149..9368)
                     /locus_tag="ACL297_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGR0071204.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIMEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
     gene            9834..10697
                     /locus_tag="ACL297_000921"
     CDS             9834..10697
                     /locus_tag="ACL297_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0071205.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKK
                     EVEIYYQAKER"
     gene            complement(10986..12377)
                     /gene="argH"
                     /locus_tag="ACL297_000922"
     CDS             complement(10986..12377)
                     /gene="argH"
                     /locus_tag="ACL297_000922"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGR0071206.1"
                     /translation="MAKNTKLWGGRFEGTVEDWVERFGASISFDQKLAKFDVIGSLAH
                     VQMLGQTGILSLEESEKIQVGLKELLEELEAGQLDFDIANEDIHMNMEVLLTEKIGPL
                     AGKLHTARSRNDQVATDMHLYLKEQLGYVLDKLAHLKGVLLDLAENHVATIMPGYTHL
                     QHAQPISFAYHLMAYYNMFQRDSERFEFNQKHTDLCPLGAAALAGTTFPIDRQLSSDL
                     LEFKQPYTNSLDAVSDRDFILEFLSNASILMMHMSRFCEEMINWCSFEYQFITLSDTF
                     TTGSSIMPQKKNPDMAELIRGKTGRVYGHLFGLLTVMKSLPLAYNKDLQEDKEGMFDT
                     VETILNSLDVLAGMLSSLQVNKEKMQESTEKDFSNATELADYLAGKGLPFREAHEVVG
                     RLVLDSIKSAKNLQDWTLEELQTYHSLITEDIYVYLQPKTAVQRRNSLGGTGFDQVEY
                     QIAVAKKANEAKK"
     gene            complement(12406..13603)
                     /locus_tag="ACL297_000923"
                     /pseudo
     CDS             complement(12406..13603)
                     /locus_tag="ACL297_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
     gene            complement(13620..14426)
                     /locus_tag="ACL297_000924"
     CDS             complement(13620..14426)
                     /locus_tag="ACL297_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0071207.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLVTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYSVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(14633..15274)
                     /locus_tag="ACL297_000925"
     CDS             complement(14633..15274)
                     /locus_tag="ACL297_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071208.1"
                     /translation="MIELENITKTIGGKVILDRLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(15271..17379)
                     /locus_tag="ACL297_000926"
     CDS             complement(15271..17379)
                     /locus_tag="ACL297_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGR0071209.1"
                     /translation="MKKISHLCMLLLLLCTTFFVLNVNVTREIVRIQEMGKTTYSFDL
                     YLKDVTKPTETILQFFEEVASQDKVSIIKVDNGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLFSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     ALASRMEKRSLVELLKGGIL"
     gene            complement(17434..17715)
                     /locus_tag="ACL297_000927"
     CDS             complement(17434..17715)
                     /locus_tag="ACL297_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGR0071210.1"
                     /translation="MNHEKISVASLMVCSILASTSVLAVWVDGGQWNYGVGWSGNFGY
                     SDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            18127..18453
                     /locus_tag="ACL297_000928"
                     /pseudo
     CDS             18127..18453
                     /locus_tag="ACL297_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(18505..19092)
                     /locus_tag="ACL297_000929"
     CDS             complement(18505..19092)
                     /locus_tag="ACL297_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGR0071211.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            19334..20005
                     /gene="sdaAB"
                     /locus_tag="ACL297_000930"
     CDS             19334..20005
                     /gene="sdaAB"
                     /locus_tag="ACL297_000930"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HGR0071212.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            20014..20886
                     /gene="sdaAA"
                     /locus_tag="ACL297_000931"
     CDS             20014..20886
                     /gene="sdaAA"
                     /locus_tag="ACL297_000931"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HGR0071213.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            20908..21540
                     /locus_tag="ACL297_000932"
     CDS             20908..21540
                     /locus_tag="ACL297_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGR0071214.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(21846..23696)
                     /locus_tag="ACL297_000933"
     CDS             complement(21846..23696)
                     /locus_tag="ACL297_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGR0071215.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(23738..24895)
                     /locus_tag="ACL297_000934"
     CDS             complement(23738..24895)
                     /locus_tag="ACL297_000934"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGR0071216.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENVEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            24953..26119
                     /locus_tag="ACL297_000935"
     CDS             24953..26119
                     /locus_tag="ACL297_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HGR0071217.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            26258..26584
                     /locus_tag="ACL297_000936"
     CDS             26258..26584
                     /locus_tag="ACL297_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGR0071218.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            26571..27164
                     /locus_tag="ACL297_000937"
     CDS             26571..27164
                     /locus_tag="ACL297_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HGR0071219.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            27157..28068
                     /locus_tag="ACL297_000938"
     CDS             27157..28068
                     /locus_tag="ACL297_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HGR0071220.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            28131..29195
                     /locus_tag="ACL297_000939"
     CDS             28131..29195
                     /locus_tag="ACL297_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6574 domain-containing protein"
                     /protein_id="HGR0071221.1"
                     /translation="MKQEWFESNNFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(29267..30127)
                     /locus_tag="ACL297_000940"
     CDS             complement(29267..30127)
                     /locus_tag="ACL297_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HGR0071222.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDDSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLAQEK"
     gene            complement(30249..31235)
                     /locus_tag="ACL297_000941"
     CDS             complement(30249..31235)
                     /locus_tag="ACL297_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGR0071223.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(31507..32982)
                     /locus_tag="ACL297_000942"
     CDS             complement(31507..32982)
                     /locus_tag="ACL297_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0071224.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(33240..34163)
                     /locus_tag="ACL297_000943"
     CDS             complement(33240..34163)
                     /locus_tag="ACL297_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGR0071225.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(34177..35106)
                     /locus_tag="ACL297_000944"
     CDS             complement(34177..35106)
                     /locus_tag="ACL297_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071226.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(35425..35646)
                     /locus_tag="ACL297_000945"
     CDS             complement(35425..35646)
                     /locus_tag="ACL297_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071227.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(35600..35860)
                     /locus_tag="ACL297_000946"
     CDS             complement(35600..35860)
                     /locus_tag="ACL297_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071228.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT        11033 a         7588 c         6055 g        11265 t
ORIGIN      
        1 atcagcaact ttattttgaa gttcatcagc ttttttatcc aactcagctt cttctgcttc
       61 tttatctaat tcatcctgag tcttaccttc aggatcaagg ctgtcaagaa gtttttcaag
      121 ttctgtttgt ttttttgcta actcagcttg tttagcgttt agctcagctt ctcctttttt
      181 taatttagct tctataactt ctgtgccatc atcaggatcc gcaccagcaa gaagtttttt
      241 caaattatct acttgatgtt gagcagtagc aacttcttgt tccaaagtag aaatttcata
      301 ttgaagtttt tcaatttcaa gttccttagc ctctacttct ttcttcgcta gtactacctt
      361 tagagttgca taatcatatt ttctcttagc tactttttct tctgctttag cttcttctgc
      421 ttttttctta gtctcagcca actgctctgg ctcaggaact accattgctt gaacagattg
      481 aaattttgtc tgagcatcgg ttagtttagt cgtctcctca gttatttttt tatctgcttc
      541 tgctaatttt ttagcgtact ctggatttcc tgaatgttta tttttttctt cttgtgcttt
      601 tctttgttcc acataagctt tttgcaccgc taaactagca tcatctactt ccttagcagc
      661 ttcttctgct tttttcgctt tctcttcagt tttcttctga tcctcatcat atttcttctg
      721 agcgtcttct gctttctttt tagccgtttc gtaatctttc ttggcagtat cagcttttgc
      781 ttttgcttcc tcatatttct tctctaatga agatttttcg acaacttgtg gagcttcttc
      841 tgctcttaca aaagtaggct gagacgtaac aaaaccagcc cctaagatag cgacgctggc
      901 tagacttgtt aaaatcattt ttttcttatt catctaaatt tacctctttt ctgatagtca
      961 tttttagact atacttatat taagtatact ccccccgtaa atatttgtca aatgttttat
     1021 attacatttt tattacaaat ttctatatca taagcttcta ctccaaaaag ataaagacac
     1081 tacttttgcc cttttcgatt ctctttttaa acatagcatc atcttccttc attcctataa
     1141 aaacatttcc gatgagagag cttgtttttt aatttcgtta aacaacaaaa aacgcaagct
     1201 taagcctgcg gacaatcttt atagccattt catttctttc acatctgttt tacgaacttt
     1261 agcatatttc ttagcgcttg taaaatgtag agtaactggt ttaaatactc cgcttttgtc
     1321 tcttcgtaaa cctaaaactg cggatctttt atttttatcc tcaaaaaata tgacgtccaa
     1381 tttcatagta tttttagata aatccttagc tacgatgcaa caacttgtta ttttaaaata
     1441 gaaaatatca cctataaact catataaaga tatcctttcg attacatcat tgtacaatag
     1501 gatatttcta aaatcttcta aaataaaaac accagcttga atagcaggtt ttgtttgact
     1561 agcataatct cgtgtgattt tgtgtaagcc aaataaatga tgtaaatcag tcaggcaaaa
     1621 attcactttg aaagtctcta aatacttata attcgttgat acttgacatg cttttctatt
     1681 gaatctttct tgataatcca aaatacaatc aagtaatttc aacgacaaac tccatatttt
     1741 ctcaaaataa aaaagcacgg ggcagttctc actactgccc aaagtggttg attagagtcg
     1801 gcttctctaa ctccctatgt tgtgcttatt aggcttatcg caagggaaca gtttttagct
     1861 ctgccaagcc acatagtgtg cttactacta tgtgtccatg tcaccatgaa ttgccgaacg
     1921 gtttaacgag aaagtatagt agattgaaac aagatgtgaa caaatcgatt aggaaagtca
     1981 aattaatttc tagaaatatt ttaacagtca taacggacta ttccagattc aatctactat
     2041 attactttta ggtttatccc aactcaatta tgacatcttt ttttcaaaag tcaatatatc
     2101 tcactctttt caacgacaag aaagaggctg ataatcgacc aacctctgtt tttagataca
     2161 gttcaagtcc aaactattct ttatagaaaa gtccgataaa ttcgagcaat agttaacata
     2221 cttacaaata taactcccaa aatagataac actattttca caaaactcag ttccgaacct
     2281 atgacatata tatgaaaaag aggggctagt cctacacata tatacaataa gtagccaaca
     2341 agttttatct caccaattcc tgcaaaaaaa tgtttcatgt aacgattctc catttactct
     2401 attaaatcaa ttttatttta gactatcata acacaatcac ctcttcaata aaaaccattg
     2461 gcgaaatact attctgaagc attgatagct cagatttctc agaatagagc tgtcctaact
     2521 atcgagcagc tcaaatacat atatatgagc aattctaagt ttagaattgc tataaaataa
     2581 atgtttccag gctattctaa gtctttgact gcttaaaatt ttacgttctg aaggattcta
     2641 aagctagaac agctcaaaat cacgagaact aaggtattcg aaacttcaga gtaatacaat
     2701 ccccatagga attctccatt cttattaagc atgattaaaa tgtaatccta acaagtgatc
     2761 ctcgactgtg tttgtagcac ttagttacct agataagact atttcccttt atcatatctg
     2821 attttaagcc gttctaagat ttaacctatg tttctattac ttgctatttt taaataattc
     2881 gaattccttt tctttgataa atggaactga aaaattagaa aaaagaagta aaaccgaaaa
     2941 caaatagcct gctttttgaa tcactaaaag atatcctaaa aataagtaaa ctataacatc
     3001 caatgcgaaa agaaaaaata ttctcctctt cattaaagta cttgtgtcaa ataatctgat
     3061 gaataaaaat tttatcgtag tatctacaca aatttttatc aaaaagataa gagccaatac
     3121 acgattatca actgtaccgt aaagaataga tggaaatata gttaatataa aactagaaat
     3181 ctctaaatat tttatccaag gagattgaga caccttgctt ctaaactgac taattttctt
     3241 atattcgtta gtcaatttag tagcaaaatc ttccccaatt aaatgaaaga ttgctaactc
     3301 taggataaaa acacttatta atacggtata agtcaaaaaa ctttctggga aaaacttatt
     3361 gattaaaatg gggaaactca ttaacagaat cgaatttatg gctagacaga tagatactat
     3421 tttatttttc acagaatata gaggtagtga tactattgag tagataaaag ctaaaaaaat
     3481 aagcaaaaaa tactccgtgt catttataat cgccctgaat gataaaaata tatacatcca
     3541 acatacgata atatgtattt ttcgaatctt atctacatta ttcatcttac tgtcgcagat
     3601 ataaatccat cccaaggata agggttcccg ctaatatcac aacctctatt aagagcacca
     3661 tatccaatac agtaagctat ttttcctgct gctgcaccaa taagccagcc tccaagacct
     3721 ccaaccttaa tatagtttat tagttttttg gttaaaactg cagctccagc taatgctata
     3781 aaagttcctg aactcatagc taaagctacg caaattcctt gacaagcacg cttgcttaaa
     3841 taagctcccc catcaagata catcatttct tcttcatcaa tcacaacata attatttggt
     3901 aatacaagtt ccatttgttt tctcctcctt agttgaaatt gtttcatttg ttttacaaat
     3961 aggacgaact taaactatct ttttattact cagtaataat ttccgagatt tcattttatg
     4021 atactctcta ttcatacaga aatcaaaatt ttgccttaac caacttgtat aatagaaaaa
     4081 atctaattga tttattctga tgagctgtat tctaatttta tcaacctttt cttgccagtt
     4141 gttattgtta ctaatagaaa tctcatacag cagtaactcg acaagacctg tacacagaag
     4201 ggtgttatga gaatcttcaa ttttactcag aatatcctca aaaagttcac tatccgttaa
     4261 aaaggtggat tgattcatcg catcatacag ctgaagagac attaagtcag aataactgag
     4321 atctgatttt tgtattttat cctttaacgt atttgtcaaa tcactttcaa acgaatcttc
     4381 tttttcccat ttattttgat tgaaaagaaa ttccgtctta ttaaaaacga gatgattgat
     4441 atccattata gtccaaaaaa atattgtagt aagcataatg atgacaaaag gaatttttaa
     4501 aataaatact aggataatag caatttcaag aataaaactc catataatcc tagatcttaa
     4561 atcatcaggc gacaaacttt tacgatggaa tgaaaaatag ggcgagaacc tcacatttac
     4621 tttcttcctg cctataaaaa taaaaggaaa aatcgtaata agagcttctt tcttgtacaa
     4681 atctttcaat gcgtatagta tgtctaaaac tatttttgat aaaatcaaac tacagaaagc
     4741 aagactcata ctaatcacaa aatcaagggg agattctaga ttcaatagta agttattcac
     4801 taggacatgc aactctagcc acaagacaaa aagagggagc attagtaaga aaacaattag
     4861 tttacgcatt ataacaatta gtaaaattta cgcgtccata aagcatacgt aattcctgca
     4921 actgaaatac ctccaattac agccaatgcc gcattaacag ctgctactga ccctagtatt
     4981 ggagcgaaag cagaaacaac ataagtataa gaatacactc ttgtagacca tattgcagcc
     5041 cataaaccag tttctcttgc ggcaaaccta aatcctgagg aagctctcgc taaacctttt
     5101 atattatttt taaatgtgtt ccagtctccc ccatcaaggt acatcatctc ttcttcatca
     5161 atcacaacat aattatttgg taatacaagt tccatttgtt ttctcctttt tattaagcat
     5221 ggttaggatg gtttcctaac aagtaatcgt cgtctgtgtt tctatcactt agctacctag
     5281 ataagatatc ctttctctac cttatttgtt tttataggtt acctaaggtt taaaaccaat
     5341 tattgcttga aacttaacat ttctaaaatc caatcaagat aggtttcttt atcatagaca
     5401 atccgcactt caactggcat atccttttgg aggacgactg tctcacttcc atgttttaga
     5461 gttaaggttt ctaattctat catgacttta taaagactaa tattgccttc tttcgtttct
     5521 tgggaaattg ttcctgaatc aatctgtctg acttgtccct ttagtgttcc atatttttga
     5581 ctatttacac cagtaatagc aacatcagct tttgctcctt tcgaaacacg agaaatatca
     5641 ctcgcaagta caaaagcttc tacatagtaa cctttttctt tccctgaaac ttctgctatc
     5701 gtttgcccct gctgaataga cattccttgt ttcaaaggta acagataatg cagagtacct
     5761 tcatttggcg ctaatatact atgagcttta tcttgacctg ttgccactcc aagattagcc
     5821 tcaagctctg taatactttt attgttgttt gaacgagcag tacctaactc actaactaat
     5881 tgagcataaa tattataagc ctgagaagtt ggatcattat aggtacggtt catatcctta
     5941 atattgcttt gttgactatc aatctcgcta ttcttagaag taatatttgt ttttaatgct
     6001 tcgatttttg ttgttatttc tgatatctta gtttctaact tagctttgtt atcagctgat
     6061 gtcgtatctg atagagaagg tgagtctcct tgctgttgaa gttgaagcaa ttcattttgt
     6121 agagcttgca agtcggcatc taactgattt ctttcagctg ttattttatt caacttcgta
     6181 tattcatcct gaatctttga atactgggta ccattattat aattttccga atttaactga
     6241 gataggtagt attcaacttt cccataatat tcctgttctt caccagaatt agataaatga
     6301 ttgtatttct catttaatga ttgttcaaat ttttgaaaga tagcttcttt tgcacgtaac
     6361 ttctccaatt gtttatttaa agagctcgct tgtaaacctt cttgcccact tgataacgaa
     6421 ataatgacat ctccagcatt taccatatct ccttctgact tatgtaaagt aactaccttc
     6481 cctgaaccaa ttgctgatag gaactctgta cctgttataa ctgaatttcc attcgctttt
     6541 acaatatagt ttttgggtat ataagctgcg ccaaccaatg caccgcttaa gataatagca
     6601 gttgaaataa tgagaataaa cgcaaaagct ggtggtctct tatcaaagaa aatacgagaa
     6661 taacgtaatt ctgatttatt atataatttc ataggcttac aattggtcta aaaatatcta
     6721 ctaccatttt ttcaggagaa gaattaacat aaactgtata gacaatccca tccgtttgaa
     6781 tatcattttc atagacatat agatccaatt tagaatacgc atactgtaga tactctggac
     6841 tgtcttcaaa acgaacatat aaacaatatg gaacagagat agaatcctgt acatcataaa
     6901 tgttactgta ctgttgagca ttatgagctt gaatataaaa ctcaaaatca gtcgttatta
     6961 atccatcatc atgaatagta gtaccacaac tttttacaat taatggacca aaaatttgtg
     7021 cttttaacaa ctgcaaatgt tgatgaaatt tattaatttc ctgatctaca tcttctactt
     7081 ttgtatcatg taacttttta cagataactg actttagtac cagtttttta ttatctttta
     7141 cctctaactt agccataagt aacctcctct gtatctaaca cagcctgtga ctgaatttgt
     7201 tgattcactt gaacgctctg caaaccaacc attctagcat actttccatt tttcgccatt
     7261 agttcttcat gagtaccata ttccataata gtcccatttt ctaaaaagca tatcttatca
     7321 catcgaagaa ttgtagacag cctgtgggct actacaattg ttgtcttatc cattatttta
     7381 ttaaagatta aatcctgaat aatctgttca ctaaatgaat ctaagttaga ggttgcctca
     7441 tcaaatatat acaaatcagc tttactcagt agtgctcttg caatagccaa tcgttgtttc
     7501 tctccacccg agaaattttg accgccttct tctacaaagg agccataacg attttgaaga
     7561 cgttgaatcg tatcatgaat accaacaata cgacaaactc tcaccatatc ctcatatgta
     7621 acagaaggat taccaatttt tagattatca ataattgtcc ctgtaaagag ttctatggtc
     7681 tgaggaatgt aggaaatttt cttacgtagt ttaccatagt caagatctga taagtcattt
     7741 tctcctatac gaattttccc ctcactcgga tttataaatc ttaataacaa acgaactagc
     7801 gtactttttc ctgctccgct ttctccaaca aaagctattt tttctccttg tttaattcgc
     7861 aaattaaagt ccttgataac aggagggcgt gaaccatagg caaatgttac atctttaaat
     7921 tcaatgtcat cacataaggt gaagtctcta atactataat ccaaggcaat gtcttcacga
     7981 tctacttcca tcaattcttg aagccgacta accgcaacct gtacctcttg aaaggttaac
     8041 tgcaagccaa ctaaattctg aataggttct gtaaaatatt gacttaatgt ctgaaaaacc
     8101 aagaggtccc caatagtcat ttttccatca atgataaata aggcgcccac tcccataaat
     8161 aataacccac ccatggttga ggtcattgaa gatattgtag attgaatatt ctgcaaaaca
     8221 ccctctttat aaccaatttc taaagtatga acaaattttt cttcaatttt gtttaaacgt
     8281 tgttcctcat catgctgaga cttaatggta tcaatgttac gaatagattc aattaactgt
     8341 gaattaagaa gtccatttgc ttccatttgt tcatgattaa tttttttata aggcttttta
     8401 aaacagtata taagaatgat gttaacaata accataaaaa ctaatatcag aaataaagat
     8461 tgattaatag tccaaaggac aaccgcacta ataactgaca aggtgatatc catgactaat
     8521 gaaatagaga ctgacgtaaa cacattcttt atcgtcatcg catcttgaaa tcttgttaaa
     8581 acatcaccaa ctctacgact tccaaaaaaa ctataaggaa gatgaataat atggtcataa
     8641 tatcccatta aaaccggtat atcaattttt cgagatagaa acaaaagaac gtgctgacga
     8701 aatgcagata gaagagtctg taagaacgac actattccaa atactatcaa aaacatatac
     8761 agactatttt ttaaagcata tggaatcacc tcgtccatga taacttttga aaagagactc
     8821 gataaaatac caatgatact taaaacaaag gaagctaata tgactgtact aatcaatcct
     8881 ttttgattaa aaattaacct tccaaataaa tcaagaagtc cttttccctt tagtttccct
     8941 ctttcaaaat ccgaatttgg aaccataaaa agagtaatac cttgataaat ttctcccagt
     9001 tcgtcacttg acatcttgcg tatgccacta gcagggtctg ctacgtagaa cttactattc
     9061 cttttgatac tatgtagtac cacaaaatga ttttgaccta aatcattctt aacttgaaga
     9121 atcgcaggaa atgttaattt tggagtcaaa tcttccagtg ctactcgaac agcttttact
     9181 gtaaaattca acttattcag acctgaaaca ataccactga cagtcgttcc atacatgtct
     9241 gtaccaataa tttccctaat cttcatgatt gatagttcct ttttgtaaga taaaagaact
     9301 gttgaaacaa ctgccgcagc acaatctgac tggtcatgtt gcatcgtaca aggatatttt
     9361 tttaacatag aaatactcaa ttctttagaa ttaatctaac ttttctttga tgtcacccaa
     9421 acctcaatca ctgcccagta gttcttgaat taatttattt tcaaccgtat cattctttgt
     9481 tcttgatatc tttccaagca ttgtagtaca gcagattttt gcccaaggac tgagcctcct
     9541 ttaacaactc aaactccctt acattatatc ggccagcccc atggaaagat tatagccaga
     9601 ctaatcaatg atataataca tactagaata caaaatattt ttttattcat actttttctc
     9661 ctatctttat tttctaccta catagtacct cttattaagc tcattttaaa attacgttaa
     9721 cttttttcac aaatttattt cgactttctt ttttactttt tacattgcta tgatacaata
     9781 tttttaacaa aattttattg tttgttaact tttttcacga aaaggagaaa atcatgcgtt
     9841 atgatttcgg aaaggtctat aaagaaatac gtgagtcaaa ggggctgacc caagaagagg
     9901 tctgtggggg tgttctctca agaaccagct tatcaaaaat cgaaagtggc aagacaaccc
     9961 caaaatatga aaatatggaa tttcttctcc gtcaaatcaa tatgagtttt gaagagtttg
    10021 aatatatctg tcaactttat caaccaagtc aacgcacaga gattatgcaa acctatctca
    10081 atatgcgttc cattatcggg accagcgatc ttgtcaattt atttcaaaaa tgccaagact
    10141 acctcaagac ccaccacgat ctccctatag aagaaatcag agatatgctt gaagttgtta
    10201 tttatctccg tcaacacggg gcaggggaac tatcaaaaca cgcagaacag gttgtcaaaa
    10261 aactttggaa aaaaattgaa aaacaagata catggtatga gagtgatcta aaaatcctca
    10321 ataccattct tttcagtttt cccattgaat acctccatct catcactgga aaaatattgc
    10381 aacgcctaga agtctataaa aactatcaac atttatatga cttgcgaatg acaattctat
    10441 taaacctttc cacactctat ctatacaatc aagataaaaa catgtgtaag caaatctgct
    10501 acactttact ggaggacgcc aagaataaga aaagctacga taggctggct atctgctatg
    10561 tccgtattgg gatttgtagg gatgatgcga aacttatcca aaaagggttc tcccttctgg
    10621 agctaaccga ggaaacttct atactgtctc atctcaaaaa agaagtagag atctattacc
    10681 aagcgaagga aagataaaaa agccgaggga tttcctcgac tctttacata ttatttgttc
    10741 gttagttctt aataccagcc attgttaaac cagaagtttt tagctctgcc aagccacata
    10801 gtgtgtttac tactatgtgt ccatgtcacc atgaatcgcc gaacgattta acgagaaagt
    10861 atgactttta cgtttatccc aactcaatta tgacattttt ttcaaaagtc aatatatctc
    10921 actttttcaa cgacaagaaa gaggctgcca atcgaccaac ctcttattct gaacccatca
    10981 ctccatcact ttttagcttc attcgctttc ttagcgactg caatctggta ttcgacttgg
    11041 tcaaaccctg tgcctcccaa ggaatttcgt ctttgaacag cagttttagg ttgcaagtag
    11101 acatagatat cctcggtaat gagagaatga taggtttgta attcctccaa agtccaatct
    11161 tgaagatttt tagcagactt gatagagtct agcactaatc ttcccacaac ctcatgagct
    11221 tctctaaatg gcaagccttt ccctgccaaa taatctgcca actcagttgc atttgaaaag
    11281 tccttctctg ttgattcttg cattttttcc ttgtttacct gcaaactaga taacatacct
    11341 gccaacacat cgagggaatt taaaatcgtt tcgaccgtat caaacattcc ttccttatct
    11401 tcttgcaaat ccttattata agctaaaggc aaggacttca tgactgtcaa cagtccaaac
    11461 aaatgcccat aaactctgcc agtctttcct cgaatcaact cagccatatc tggatttttc
    11521 ttttggggca taatagagga acctgttgta aacgtatctg acaaggtaat gaattggtac
    11581 tcaaaactac accaattgat catttcttcg caaaaacgac tcatatgcat catgagtatg
    11641 ctggcatttg atagaaattc taagataaaa tcgcggtcac taacagcatc caaagaattt
    11701 gtataaggtt gcttaaactc cagcaaatcg ctagacaatt ggcgatcaat ggggaaagtc
    11761 gttcctgcca aagctgccgc acctaaaggg cataggtctg tatgcttctg gttaaattca
    11821 aaacgttcac tgtctctttg aaacatattg tagtaagcca tcaggtggta ggcaaaacta
    11881 ataggctggg catgttgcag atgggtatag cccggcatga tcgtcgccac atgattttcc
    11941 gccaagtcta ataaaacacc cttaagatga gccagtttat ctaggacata gcctagttgt
    12001 tccttgagat acaagtgcat atctgtcgca acttggtcat ttcgagaacg agccgtatgt
    12061 aacttccctg caaggggacc aattttttct gtcagcaaca cttccatatt catatgaata
    12121 tcttcgtttg cgatatcaaa gtcaagttga cctgcctcta gctcttctaa aagctctttc
    12181 aagcctactt gaatcttttc tgactcctcc aaactcaaaa tgcccgtctg acccaacatc
    12241 tgaacgtggg ctaaagaacc aatcacatca aatttagcta atttctggtc aaaagatata
    12301 ctcgcaccaa agcgctctac ccagtcttcg acagtacctt caaagcgacc accccataat
    12361 tttgtattct tagccatatc acatccacct ctctatccaa tcgtcctatt tggcgctttt
    12421 ttgaacctca gaatgaacct tggttggaag tccccataac tcaataaagc caacagccgc
    12481 atcttggtca aaggtatctg cactagtata cgtcgccaaa ttttcatcgt aaagagaatt
    12541 tggtgatttc cgagccacga cctgagcgct cccccttata cagtttgact tttgcagtcc
    12601 cattgacaac tttttgagtc tctttaatat aagcaatcaa ggcttgtgta gccgaactaa
    12661 accacaaagc attataaatg agatttgata attcattttc tatgattggt ttaaaatgag
    12721 ccacttctct cacaagcgtc aagtcctcaa tttccttatg agctgtcagc agggtcactg
    12781 cacctggaca ctcatagatt tctcttgatt taatcccaac caagcgattt tccacgtggt
    12841 caatccgtcc aaccccatgt tttccagcta tttcattgag tttttgaatc aaatctgcca
    12901 attttaacac ttctccattg agggaaactg gaacaccttc actaaactca atctcaatgt
    12961 attctggcat atctggagct tgctctggag aagttgtgat gccaaaggct tcttctggag
    13021 cctgattcca tggattttcc aaaataccac actcattggc acgtccccaa agattttggt
    13081 cgacagagta gggattgtca aggtcagcag gaacaggcac cccatttttc ttggcataat
    13141 aaatttcttc ctcccgagac catttccatt cacgaactgg agcaatcact tttaaattgg
    13201 gatccaaggc tgcaatcgat acttcaaaac gtacttggtc attcccctta ccggtacaac
    13261 catgagcaat tgtagtcgct cctatctgat gcgctatttc aaccaatttt ttagaaatca
    13321 gagggcggct caaggcagat accaagggat acttttgttc ataataggca tgtgactgat
    13381 gagccactag cacataatct gtagcaaatt cgtccttaac atcaatgaca taagattcta
    13441 ctgccccaac cttaagagcc ttatcatgga tgaaatctaa gtctttccct tcacccacat
    13501 ccatacaaac tgaaacaaca tcatagtctt tctttaacca ggtaatagca actgatgtgt
    13561 ccaaaccgcc agaataggcc aaaattactt tttctttact catgttcttt tccttctttc
    13621 tatttttcaa tggatgcttt aaaggcatcc tcaatgagtt tgtctaattc cccagactcc
    13681 ttcaactttt gaatagtttt atcgactgcc tctttcaatt ccttactatc ttttttcatg
    13741 gctaccgagt aggaatcatc ttgctctttt tcaaaattga ggtctgcgat tgctaaatca
    13801 ggattatttt ccacaaagcc cttggcaact ggttcttcaa agataacggc atccacttgt
    13861 cctgatttta aatctgtgat taaattccca tttttaggca gagatacgag ggaagaattt
    13921 tgtagcaaat ctttcgccat cgtctcttga atcgaacctt tctgggctcc gaccttcttc
    13981 tgcgctaagt cgttgacaga ctgataagtg actaaatcag attttttgac aatgagttta
    14041 ttttttgcag tatagtaggg aatggaaaag tcaaacacct tgctccgttc atctgtctta
    14101 gaaacacctg atatggcaag atcagctttt cctgattgaa cactagccag tacattgtca
    14161 aaactcatgg gagatagttc caattcgaca cctagttctg ttgcgatagc cttggctaat
    14221 tcgatatctg aacccacaat ctgatttttc ccatcaacca ctttttggta ttcaaatgga
    14281 gcaaaatctg gattgagggc tacaaccaat tttcctttgg acttgatggc ttcaattcca
    14341 gccgattgat tgttactaca agcaaatagt agggggagca tcactaaacc aaacatcgtc
    14401 atcaacacct tcttcatctt attcataaca aagcctcctt tatgtttatt ccttttgttt
    14461 gtataaataa tacacctatc ttcatccttt gtcaagccta aattgtaatt tttttcaatt
    14521 taaaacagaa aaacctagga aaattacata aaaatgtcat tcctaggcgt atttatgcta
    14581 ttcctctctg gaaaatatga gtattcagtc agtcaaaaga agctgaacga actcattttc
    14641 cctcgcccaa ttcaataatc cgatgacatt gttgggctac ataagcatcg tgggtcacaa
    14701 taatgactgt tttcccttct cgattcatct ctaagagaaa cttcaagacc aaatctctat
    14761 tttcaggatc cagcgaacct gtaggttcat cggctaaaat cagctggctg ggttttaaga
    14821 tggctctagc aactgcaatt cgttgttgtt cgccaccaga caactcggag accttttgat
    14881 gcaaagtagc tgacaaacct actctctcta aaatctcttc cacctttttg agcttatctt
    14941 tcttagacaa ttttacatat ttcagcgcca gcataagatt gtactcgacc gtttcatcat
    15001 caatcagggc aaaattttga aacagataag agatatgttc acggattatt gtttgcgact
    15061 tagcagaatt gaccgctaga tttgtctgac caaaaatctc ataccgtccg ctataatcac
    15121 catctatcaa acccaataaa tttaacaagg tcgacttccc actaccactc ttaccaacaa
    15181 tggcaaccaa atccccctga tcaatcctga gagacaagcg atccaaaatc actttccccc
    15241 caatggtttt ggtaatattt tccaactcaa tcataagatg ccccctttca ataactctac
    15301 tagacttctt ttctccatcc tagaagccaa ggctagcaca aatagtatat ccaaacatgt
    15361 aaagcctgca aacagcagga gtggcaagaa cgcatgggca aagaaaatca agactagaag
    15421 agggagacta tagcccagca agagcagaac gaggagagga cggtagcgat cgaccagttt
    15481 ccaccccata aacttcttgg taataatatc cctgcgcttc aataagaaag ttattactag
    15541 taagaagtag gaaatcatca tgctaaagag accaaacaaa gcaaagagta ggttaaaatt
    15601 ccgaacagca tctcgataag aatccacttt ctcttgttga atggcttgaa tagatgaaaa
    15661 ttttaaataa tttccatctg acaatttctc aactaactct gtaatctctt tttgatgttg
    15721 aaccgtattt tcaattttaa tcggattatt taagccagtt gttgacaggg aggctttctc
    15781 atcccacatc atatcagaat cattgaccaa gctaataatt ggattggaga gattttcctt
    15841 tcgcttatca ctatatggga aaaatgacca atctccttca taataggcaa tctcgacatc
    15901 catctcctct atcgttcgtt tttgctgctc ttcatacttc atcgaatgaa aggcaattaa
    15961 cttccccaag agctgatttt tatcttcttc acctttcgta cttgctggca tcaaaataac
    16021 ttttttaata ccagtatctg gtagcttgaa tcccttgctc tttagaaaat tgcgattggc
    16081 atagtaaaca tccaccgtat ctgttaactg atattgctga atctgttctg attggacaaa
    16141 attttttaca ggaagactgc tactctgcac atagcccgcc tgcgtttttt ctactaaatc
    16201 ctgataaaat cgatagaaat aatccaatgc cttccctgag cctgccaact gctcttgcca
    16261 caagttgtca ttgagttgga aagtttctaa ggtcagataa tttccttggc tgatccactg
    16321 tttctggtaa tccaactctt tgttttcttg ctccaaactt tttcctaccc caactagcaa
    16381 gactgtcagt aaaatggtag ttccaatttt catcaaataa ttaaagagta gaccaaattt
    16441 gaaagatgaa aaacctttca gcagagagct gattgtcatt ttttggatta agaggtaagt
    16501 caaccaactg ataaagagat aaagctgcaa cagcaaaaaa tgagacaacc acagcatagg
    16561 aaacaaatct tttggcttat aatcaagcaa gaaaaacacg cctagattga tcacaagagc
    16621 cccacctagg aggaggtaaa ggttgccttt tacaacatca gctaaaacag ccctatcttg
    16681 aaaaccaagt aatttttgta ccccaactcg tttcatctcc atcatcggtt gatacactgt
    16741 cactaacaca agaagcaaaa tagccaagac aaaaacaatg gcagataaaa gcaaatctcg
    16801 atttatgact tccactgcac ttttgtaggt cggctctagc aaggtagcct ggtctatctt
    16861 gaaaaaatcg ctccatttct gtacaatcct atccttgtcc atctcttgtg tagaagttat
    16921 cgtatagcga ccatttaaac tacgagatgt atccttgata taggtttgaa aagtcataag
    16981 ctgaataggt ttggctttta gaaaggtcgg aatcgtacca agtttattgg aaatttcttt
    17041 attactatag actccttcac catctgtggt aaaatcaaga gaagaaatcc caaactcttg
    17101 gtaggggaag gtatctttat caaaaacacc agacttgacc acctcatcgc cattatctac
    17161 tttgataata gagaccttgt cttggcttgc aacctcttca aaaaactgga gaatcgtttc
    17221 tgtcggttta gtgacatctt tcaagtacaa gtcaaatgaa taagttgtct tgcccatctc
    17281 ctgaatccgc actatttctc gtgtaacatt tacattcagg acaaaaaacg tagtacacag
    17341 caagagcagg agcatacaaa gatgactgat ttttttcata gagatacctc ccgtgaagaa
    17401 actccctctc caaaagggga gggagaattt taattaaaat ccatagaaat attccattcc
    17461 cgttggtggg aatttgaaga ttgaagcccg agcccacgcc tcagcttctg cacgatcctt
    17521 agagaattta tttccatctt taacagttgc tgtatgagag cgagtagaat gtaaataatc
    17581 agaataacca aaatttcctg accatcctac tccatagttc cattgaccac catcaaccca
    17641 tactgccaaa acacttgtag aagcaagtat actacatacc attaaagatg caacactaat
    17701 tttctcatga tttatttctc ctattatcga gagctattcc gtagaaacag ctatagtaat
    17761 ttatcaacaa gtatcgtagt ccattggact caccttcctt ctatctcatt tcatttttaa
    17821 acaacgaact tactcttaac taggagaata gttctcttct gtatcaggtc tttgaacaac
    17881 cagagtagta ttaatcacac tctaactatt actcaagaat cagcccaata aacaatatgg
    17941 atagcgcttt caaattttta aactctatcc catcctttta tctatattat gagcgaaaat
    18001 ataataactg tcaagtaatc gaagtgaatt ttataaaaaa aattacaagc caaatttgca
    18061 aagtttacaa taagccgcta gggcaatcgt ctatcagaat gtccgtttat ttgtcaataa
    18121 tccgagaaaa tcttgcaacg cttagaagtc tataaaaact atcaacattt atatgacttg
    18181 cgaatagcaa tcctgctcaa cctttccaca ctctatctat acaatcaaga taaaaacatg
    18241 tgtaagcaaa tctgctatac tttactggag gacgccaaga ataagaaaag ctacgatagg
    18301 ctggctatct gctatgtccg tattgggatt tgtacagacg attctaaact tatccaaaaa
    18361 gggttctccc ttctggagct aaccgaggaa acttctatgc tgtctcatct caaaaaagaa
    18421 gtagagatct attaccaagc gaaggaaaga taaaaaagcc gagggatttc ctcgactctt
    18481 tacatattat ttgttcgtta gttcttaata ccagccgttg ttaagccaga agtttttagc
    18541 agctgtccat gaaccgtaac gtcctgcaac gtaggcatct gctacacgtt cttggttttc
    18601 agctgagtag tcaccgttca ggtatgaatc tgttaattgg taacgtccga tataacgtcc
    18661 atttgtagct gtatagctac cacctgattc tttttgagca atccattctt tggcttctgc
    18721 ttcagatccg cttacagttg aaacaactgt ttcacttact actggagttt ctgctactgg
    18781 agctgaaaca gtttcatctt gagcggctgg tgccgcataa gtagctggcg ctggtgttgc
    18841 aacaggcgct acagggccat cgataaccaa ctcttgacca acataaatca aatgaatgtt
    18901 atcaatgtgg ttgttttctg ccaatttttc aactgttgtg ttgtgagttt cagcgatttc
    18961 tgaaagtgta tcaccttctt taacagtgta agttgatgat tcttgagcag atacaaatga
    19021 tggagcaaat actgcaaaca aggcagctac tcctgcaaga gttgctttaa tctttttagt
    19081 tattgatttc atattcgaaa attctccttc tttctatagt tctatcatac cctttaaata
    19141 ttaccgtttc ttgacacttt tatgtagaaa tattacaaaa ttatttttta tttccctaaa
    19201 taaggtgttt tttgtcataa aagatactat ttttatgcta tttgaggtat tagaaaggtt
    19261 ttcatccaca attaaagcca agcccctcat tctttgatat aatagagata agaattttta
    19321 taaggggaaa ctgatgaaat cacttcgttt tcaatctgtc tttgatatca tcggaccagt
    19381 tatgattggc ccatctagta gccatactgc tggtgctgtt cgtattggga agattgtctc
    19441 ttccattttt gatgatactc cgacagaagt tgaattccaa ctttttaact catttgccaa
    19501 gacctatcgt ggtcacggaa cagacctagc ccttgttgca ggtattttgg gaatggatac
    19561 agatgatcct gaaattccaa atagtctgga aattgcccac aagcgtggta tcaagattgt
    19621 ctggaccatt cagaaagaca gcaatgcccc tcaccctaac accactaaaa ttactgttaa
    19681 aaatgcccac aagaccatca gcgtgactgg tatttctatc ggtggaggaa atattcaggt
    19741 aaccgaactc aatggttttg ccgtctctct caatatgaat acaccgacta tcatcatcgt
    19801 tcatcaagat attccaggta tgattgccct tgttacagag gcgctttccc gctatggtat
    19861 caatatcgcc cagatgaatg tcactcgtga aaaagctggt gaaaaagcca ttatgattat
    19921 cgaagttgac agtcgcaact gtgatgaggc tatcgaagaa attcgaaaaa tccctcatct
    19981 ccacaatgtc aatttcttta aataggagga agcatgtttt attctatcaa agaattggtc
    20041 gagcaagcag atctggactt tcaaggaaat gtcgcagaac tcatgattac aacagagttt
    20101 gaattgaccg gtcgcgaacg tgaagaagtc ttccttctca tggaacgcaa tctggaagtt
    20161 atgaaagcct ctgtccaact tggcctcaat gaaaataaat ctcgtagtgg cctgacaggt
    20221 ggagatgctg ccaaattgga tcactacatc gaaaacggaa aaactttatc agattacacg
    20281 attctctctg ctgcccgaaa tgctatcgca gtcaatgaac acaatgctaa aatgggcttg
    20341 gtctgcgcta ctcctaccgc aggaagtgcc ggttgtctcc cttccgttct cactgctgct
    20401 attgaaaaat tagacctcag ccacgagcaa caattggatt tcctctttgc tgctggtgcc
    20461 tttggactag tcatcgcaaa caatgcctcc atctcaggtg ctgagggtgg gtgtcaagct
    20521 gaagttggtt cagcctctgc tatgagtgct gccgccttga ctctggctgc aggtggaaca
    20581 ccttatcagg ccagtcaagc tattgccttt gtcattaaaa atatgctagg cctcatctgt
    20641 gaccctgttg caggtttggt cgaagttccc tgtgtcaaac gtaatgccat gggagctagc
    20701 tttgctttca tcgcagcaga catggccttg gcaggtatag aatctaaaat ccctgtggat
    20761 gaagtgatcg atgccatgta ccaagtagga gcaagcatgc caactgcctt tcgtgaaaca
    20821 gctgaaggtg gactcgctac cacccctact ggtcgtcgcc tccaaaaaga aattttcgga
    20881 gaataagctt atcaaatagg agaaaccatg acctctatca cagcgatttt tttcgatttg
    20941 gatggaaccc tcgttgatag ttctatcggg attcacaatg cctttaccta tacctttaaa
    21001 gagctggggg tgcctagccc tgatgccaaa actattcgtg gttttatggg accacctctc
    21061 gaaagtagtt ttgcgacctg cctgtccaaa gaccaaattt ctgaagccat gcagatatat
    21121 cgttcttact ataaggcaaa aggcatctat gaagctcaac tctttcctca gattatagac
    21181 ttgcttgagg agttatcgag cagttatcca ctctacatca ccacgacaaa gaatacatct
    21241 actgctcaag acatggccaa aaacttggaa atccatcatt tctttgatgg catttatggt
    21301 tctagccctg aagcacccca taaggcagat gtcattcacc aagccttaca gacacatcaa
    21361 ctagcaccag aacaagccat catcatcgga gataccaagt ttgatatgct gggagctcga
    21421 gaaacaggta ttcagaaatt ggccgtcact tggggatttg gagagcaagc agatctacta
    21481 aactatcaac ctgattatat cgctcacaaa cccttagaag ttttggcgta ttttcaatag
    21541 catagactgg gcgacaactc tatttcagaa gaaaatgaag taatactcaa tgaaaatcaa
    21601 agagcaaact aggaaactag ctgcgggctg ctcaaaacac tgttttaagg ttgtagatgg
    21661 aactgacgaa gtcagctcaa aacactgttt tgaggttgtg gatagaactg acgaagtcag
    21721 taacatatac atacggcaag gcgacgttga cgtggtttga agagattttc gaagagtata
    21781 agtctctaca taataaaacg catattacca aggttgtgta acttgatact atgcgttttt
    21841 cttttttatt caatgtgggt tgtttgatta gcaaaggcga taatagcttg cttcaactca
    21901 tctccactga gagtgcggaa ctctccaatc ttttgattga tggattctaa aggcatgaca
    21961 ttaaccttac caacgaaaat cttatccata acttgattat caacgagttc ggttgatacc
    22021 aagagttctt cgtagagttt ttcacctggg cggattccaa cttcaacgat tggaatttca
    22081 ctttcagtgt ggccacttag aagcaccatc ttcttggcca agtcataaat cttgactggt
    22141 ttgcccatat caaggataaa gacttcccca tctttggcat aagcaccagc atggataacc
    22201 agacggctag cttctggaat ggtcataaag taacgggtca tgcggaagtc tgtcaccgtt
    22261 acaggcccac cttcagcaat ctgacgttca aagactggaa tgacactacc acggctacca
    22321 agaacattcc caaaacgaac tgcacagtag gttgattggc tacgttggtt aaagccagtg
    22381 acaatcaact ccgccacgcg cttggttgct cccataacat ttggtggatt gactgcctta
    22441 tctgtcgaaa tcataaccat cttagacact ttagcttcat caacagcctt agcaacattg
    22501 taagttccac ggatattgtt tttgaaggct tcttttggat tgcgctccat cataggaaca
    22561 tgcttgtggg ctgccgcatg ataaacaata gcaggtttgt actgctcaaa gacttgcaac
    22621 aaacgatcat agtcttgaat gtccgcaatc acaggtacat aatcaatccc ttggaactta
    22681 cgaatcaatt catgataaac aaggtagatt gagttttccc catgaccgag taagacaatg
    22741 cgttcaggat tgaagcgact aacttgacga cagatttcag aaccgattga acctccagct
    22801 cctgtgacta agatggtctt acctgtcagt tctgcaccca gacgcgattc gtcaagacgg
    22861 atttcctgac gacccaaaag gtccgtaata tcaatttttt ggaagccagt acctgcttgg
    22921 tgaaggccct gaacaacagt ttcaacctta ggcatcttgt aacatttgac acccagctta
    22981 ttacacatct gcaagatacg ctcatattct gacggatcca gcgacggaat cgcaacgatg
    23041 acacgctcga tttgatggcg tttggctaat tcaggcagat tgtcataaga gcccaaaaca
    23101 ggaataccac caagtttttg accctttttc ttagaatcct tatccaaaat accgaccagt
    23161 tctaattcac tggttggatg ttggtaacta tccataaaga gagccccacc atcaccggca
    23221 ccaatcaaga aggtccgacg gtgttctcca tcaccactac cttttttgcg tctggagtag
    23281 attaactgcc aagtaatccg tggcaataaa atcaagaagg tactcaacaa gataaagaga
    23341 atgatgaaac ggatggagaa gagtggcaag aaggcataac agatactata tgacaagaca
    23401 ctgctagcag tcacaccaaa aaagattttc atgaaatccg taatcttgct gtaacgacta
    23461 atgctcgcgt tcaaccccca aaaaccaatc atcaattgat agaacaagaa ggccaaactc
    23521 gtatagataa tgtagtcaac aggtgctgga ttaatcagcc cataaaataa aatataagat
    23581 acaattatgg aaaccaccat actgaaaata tcaaatattc cccagaaaac ctgcttttgt
    23641 tgtttattta aaatttccac cagatcaatc acataatctg ttagtttttt attcatagaa
    23701 atctactcct ccttaaaaga gttttactat attgttatta tagcatattt ttatttattt
    23761 ggcttatttt atccaaataa attattgttc tcttgtactc cttcttccat ttaaatcctc
    23821 taattaaacc tacaatcgtc ccaaggccat aagcaaagtg aatggaaaat aaaagaaagg
    23881 gcatcacaat tagaaatcca tttttatgtt ttaataaagt cagcaaagtg agtaatgaca
    23941 aaagtagaaa ataggcacct aatagtaaag ttatgaatac gaatgtgatc ggtaacaatg
    24001 ctagactaaa cacaagactc aaaacaaata aacaaggaac atagtgaaat aatgataaac
    24061 acttaaactg aacatgactt gtcaagccaa tccacaaacc atttgaatac ttttgatgca
    24121 gcattttctt gaatgttggt cgaatatact gataagatag aatacttggg ctatagcgga
    24181 ttttataacc atgttctcga attctataat gaatatcatt atcttcagtt cggccaagtt
    24241 gctcatttac taaaccaacc ttctggaaaa cctctcgttt atacatccca tgaaaaatag
    24301 aagaaacata tctatcctcg gaactatttc gataattggc aatgctactg ccaaacatat
    24361 tttcctcaac aagatgcaag gtctctgccc attttccttt tccttcgaca atcgtcggtc
    24421 taggcccccc acagacaaat tcaccttgtt gaataatagc cacattgttc attacaaaac
    24481 tctcagtaac ttttgaatga gcatcaattt ttaaaataag gtcccctaca gaatgtttaa
    24541 ctcccaggtt aaaaccacta gcttgatttt tcttaggatt gttatacaat ctaattgagt
    24601 taaactctgt atcttccttt ataaattgct gaatgatagc tgtggtccca tctgtggaca
    24661 tagcatttat aaatagaatt tcaacattct ctttaggata ggtttgattt tttaagtctt
    24721 caattagacc aggcagatat ttttcttcat tgtatgcact gattacaatg gatacgagaa
    24781 gactttcaac attttgagtc actactactc tcctgaattt ttccttccac cattctatca
    24841 taattttcaa gactttccca tttttatctt gaaagccaga aaccttactt gacattatag
    24901 tgaaaaagcc ttaaaataag ctgttattaa aatcagctag aagaggtatt atatgaaaaa
    24961 gcaatcactc ttttttgttc caggtattat cctgattggt gtttccttgc gaactccttt
    25021 tactgtttta cccattattt tgggaaatat ttcgcaaggt ctggaggtag aagttagttc
    25081 gcttggtgtc ttgaccagcc tgcctctcct tatgtttacc ctcttttcac cattttctac
    25141 ccgactggct cagaaaatcg gcttggagca tctcttcacc tacagcctct tcttcttgac
    25201 catcggctca ctcattcgac taatcaatct gcccctgctc tatctaggaa ccttgatggt
    25261 tggggcaagt gtcgcagtca tcaatgtcct gcttcctagt cttatccaag ccaatcaacc
    25321 aaagaaaatt ggttttctga ccaccttata tgtaacgtct atggggattg caacggctct
    25381 ggcttcctat ctagctgtgc ccattacaca agccagttct tggaaaggac ttatcctcct
    25441 cctcacgtta ctctgtctag caactttttt ggtctggctc ccaaatcacc gctataatca
    25501 tagactagct ccacaaacca aacaaaaaag tcaaataaag gtcatgcgta ataaacaggt
    25561 ttgggcaatt attatctttt caggttttca atccttgatc ttttacaccg tcatgacctg
    25621 gttacctacc atgtctatcc atgcaggtct atccagtcac gaagctggct tgctgacttc
    25681 tatcttatct ctgattagca ttcctttttc aatgaccatc ccaagcctga caaccagttt
    25741 atctactcgc aaccgtcagc tcatgctcac tctggtttca ctagctggtg tggtcggcat
    25801 ttccatgctc tttttcccaa tcaataattt catttactgg cttaccatcc atctcctcat
    25861 cggaaccgca accagcgccc tcttccctta tctcatggtc aacttttcac tcaagacaag
    25921 cgcccctgaa aagacagccc aattgtccgg cctatctcaa acaggaggct atatcctagc
    25981 agcctttggg ccaaccctct ttggttacag ttttgacctg ttccactctt gggtaccagc
    26041 tgtagctgcc ctcttgctca tctatatcct gatgactgtg gccctcttta cagtggacag
    26101 agcggataag atcctttaaa cttctagttt tagctagaag ttttttatat ataaaaattt
    26161 tacacatttc tcttgacagg gctttctttt agatgtacaa tgtatgtgta gaaaaattat
    26221 atataaaaat cttacacatt agaaaaggag gtttcccatg tactttccaa catcctctgc
    26281 cttgattgaa tttctcatct tggctgtact ggagcagggt gattcttatg gttatgagat
    26341 tagccaaacc attaagctga tcgctaatat caaagaatcc acactctatc ccattctcaa
    26401 aaaattggaa ggcaatagct ttctgacaac ctattctaga gagttccaag gtcgcatgcg
    26461 caaatactac tccttgacaa acggtggtat agagcagctc ttgaccctaa aagatgaatg
    26521 gacactctat acagacacca tcaatggcat catagaaggg agtatccgcc atgacaagaa
    26581 ctgaatacct gactcagcta gaactctatc tcaagaaact acctgaagct gaccgtatcg
    26641 aagccatgga ctatttcaga gagctctttg acgatgctgg agtcgaagga gaagaagaac
    26701 tcatcgctag tttgggaact cccaaagaag cggcccacga agttctatcc aatcttctcg
    26761 ataaaaaaat caatgaagca cccgctcaaa aaaataaccg acaaatttta catatcgcct
    26821 tgttagccct ccttgcagca cctatcggca ttcctctggg aatcgccatc ctcgtgaccc
    26881 tgttcgcaat ccttgtagcc gctttgactg tcattctggc tttctttgca gtttccatac
    26941 tgggtatcat cggcggattc ctatttttag ttgaaagttt cactgtcctc gcccaagcca
    27001 aatcagcctt tatcttgatt tttggttctg gtttactggc tatcggtgct tcttcgctag
    27061 ttttacttgg catttcctat gtaactcgct tcttcggtct actcattgtt cgtctggtac
    27121 aatttgttct taaaaaagga aagagaggta accagcatgc gtaaatggac aaaaggattt
    27181 ctcatctttg gtgtggtgac taccgttatc ggctttatcc tgctttttgt aggtatccaa
    27241 tctgacggga ttaagagcct actttccatg tccaaagaac ctgtctatga tagccgtacg
    27301 gaaaagctaa cctttggcaa ggaagtcgaa aacctagaaa ttactctcca ccaacacacg
    27361 ctcaccatca cagactcttt cgatgatcaa atccacattt cttaccatcc atctctttct
    27421 gctcaccatg atcttatcac caatcagaac gatagaactc tgagtctcac tgataagaaa
    27481 ctgtctgaaa ctccgtttct ctcttctgga attggtggga ttcttcatat cgcaagtagc
    27541 tactctagtc gttttgaaga agttattctc cgactaccaa aagggagaac tctaaaaggg
    27601 atcaacatct cagccaatcg cggacaaacc accatcataa atgctagcct tgaaaatgcg
    27661 accctcaata caaacagcta tatcctccga attgaaggaa gtcgtatcaa aaacagtaaa
    27721 ctcacaacac ccaatatcgt taatatcttt gatacagttc ttacagatag tcagctagag
    27781 tcaacagaga atcacttcca cgctgaaaat atccaagtcc atggtaaggt tgaactgact
    27841 gccaaagatt atctcagaat catcctagac cagaaagaaa gccaacgaat taactgggac
    27901 atctcaagca actatggttc tatcttccaa ttcacaagag aaaagcctga atcaagaggt
    27961 acggaattaa gcaaccctta caaaactgaa aaaaccgatg tcaaggatca actcattgcg
    28021 agatctgatg ataatattga tctaatatcc acaccaagca gacgttgaca aaaacgctta
    28081 catctgctac tatggaagca tatttatcta actaaaaagg aggttctacc atgaaacaag
    28141 aatggtttga aagtaataat tttgtaaaaa caacaagcaa gaacaagcct gaagagcaag
    28201 ctcaagaggt tgcagacaag gctgaagaaa cgatagccga tttcgataca ccaattgaaa
    28261 aaaatactca gttagaggag gaagtctctc aagctgaagt cgaactggaa agccagcaag
    28321 aagagaaaat tgaaactcct gaaggcggtg aagcgagaac agaaatagaa gaaaagaagg
    28381 catctaattc tactgaagaa gagccagacc tttctaaaga aacagaaaaa gtcactatag
    28441 ctgaagagag ccaagaagct cttcctcagc aaaaagcaac cacgaaagag ccacttctta
    28501 tcagtaaatc tttagaaagt ccttatatcc ccgaccaagc tccaaaatct agggataaat
    28561 ggaaagagca agtgcttgat ttttggtctt ggctagtgga agcgatcaaa tctcctacaa
    28621 gtaagttgga aacaagtatc acacacagtt acacagcctt tctcttgctc attctgtttt
    28681 ctgcatcttc ctttttcttt agtatctatc acatcaaaca tgcttactat ggacatatag
    28741 caagcattaa cagtcgcttc cctgagcagc tagctccttt aactcttttt tctatcatct
    28801 ctatcctagt agcgacaaca ctcttcttct tttcattcct cttgggtagt ttcgttgtga
    28861 gacgatttat ccaccaggaa aaggactgga cgctagacaa ggttctccaa caatatagtc
    28921 aactcttggc aattccaatc ttcctcactg ctattgctag tttctttgct ttctttgata
    28981 gcctacgatt tacagctctc ttgtgtgtga ttagcattgg aatcattctg cttgccagtc
    29041 tccatatcat tacaagacca agtcaagcaa gtgaaaccga ctccttctat caattattct
    29101 tgtctgtcct tgtgaacgga gttattatcc tcctcttctt tgtagctgaa gtggcactga
    29161 ttggagatta tcttcgtatc ttggcctttc tttaaaattc aatcctgtca tccttggcag
    29221 gattttttct atgtcaaaaa agcaagtcga ttgacctgct tctgctttac ttttcttgtg
    29281 ctaatgtttt aaaatcttgt actaggatgg gttgtatttc tatttgattg gcgtctagtt
    29341 ggtggtaaaa agatgggggt aatacctcta agaattttgc atagtccaag cgagctatgg
    29401 attcatagtc ttctggttta gagtcgtctc ctgtgatttg aaccaagtca atctcccact
    29461 ggacttcctt atccaccaat tgctcaatat aggctgcagg aacaaacggt tctctcggta
    29521 aaactgtctt gactccttga gctagatggt aaacgccatg cacttttcct tctccatcct
    29581 gatagaagga aactctcgca aagtaggcat ctgtctcttg aaccgcacgc acacgtgctt
    29641 caaactgagc caagccatct gcctctaaaa agcttttcaa gttctcacga ctaaagaaaa
    29701 taggattaaa aattccttgg caaatcgtaa aacgagctac agggtctgct agataggctt
    29761 gaatacttgc ttggaaataa gcttcaaaat caaaaccatc tagcccttca atatcagtcc
    29821 ctgtataagc aagcaaagca tctaaaaatg acttgataat ctgccagtct gtctttgtta
    29881 gtaggtcagg aagatcgaca cggtaagcct tttgaccatc agcataactg accttaaaaa
    29941 gaaattgtga ttgccccact atcgcacact caatatactc gagacgatta agaggctgac
    30001 ggagatagac tacatcatag ctatgtgact ccaaaccgtc tagcaaggcc aagatcgact
    30061 tggcattcaa aatctcttgt tctcctaaaa tgctctgttt atttggaata aaaaatgttt
    30121 tcgccataac tggactcctt tgaaatcgtt tacatacagt ataccttatt tttctgaaag
    30181 atacaaaaac aaactttaga tttttgagta caaagcatag aaaaacagcc cctgaggact
    30241 gtttgatctt atacagtagc tgcctgatcc aagctttcac cgatagcggc taggcgctca
    30301 atcacttcag cttgtgtcaa ttcatttttt gaaacatagc ggttacgtgg gtgaacacgg
    30361 cactcgtgtg agcatccacg aaggtacttg tcttcatttt cttctgatgt caagatacga
    30421 cggttacaga agggatttcc acagttgaca taacgttcac atggtgttcc atcaaaccag
    30481 tctttcccta cgatggttgg gttgacatgg ttgacatcaa cggcgatacg ctcgtcaaat
    30541 acgtacattt ttccatccca aagctcacct tgaacttctg ggtctttacc gtaagttgcg
    30601 attcctccgt gcaattggcc gacatctttg tagccttcac ggaccatcca gcctgagaat
    30661 ttctcacagc gaacgccacc tgtacagtaa accacgacac gcttgtccat gaatttttcc
    30721 ttgttatcac ggacccattg tggtaactca cggaagttgc gaatatctgg gcgaatagct
    30781 ccacggaaat gtcctaggtc gtactcataa tcgttacgtg tgtcaaggac aacggtatct
    30841 ttatcaagaa gcgcttcttt gaactctttt ggagacaagt aagcacctgt tgtttcaagt
    30901 gggttgatgt cattgtcaaa gtcgttgtct tccaaaccaa ggtggacaat ttctttcttg
    30961 tagcgaacaa acatcttctt gaaggcttgt tcattttctt cgtcaatctt gaaccagagt
    31021 tcttccattc ctggaaggct gtgaacgtag tccatgtatt tttgagttgt ttcatagtca
    31081 cctgaaactg ttccgttaat tccctcgtca gcgactagga tacggccttt aaggccgatt
    31141 gatttacaga aagccaagtg gtctgcagca aattgctctg cattttcaat tggagtataa
    31201 aggtagtaaa gtaagacacg aatatctttt gccataagat ttgttctctt ttctattctt
    31261 aaattttcta aattttttat ttatctacac tagtatacct gcttgagaaa acgaatgcaa
    31321 tttatttgac tggaaattgt agagggttct cgaaatttcc tgaatggtta aaataaggac
    31381 aagagaaaac atggatatct atatccttgt gccaaaaaaa ccactgccct ccccagacca
    31441 acctgaggaa agcagtgatt cttattttag gagttaggaa tgaatacacg aaatcaattt
    31501 agctgattat tttttgtttt tcaagaattc atcgtattgt ttttgcattt cgttcaatac
    31561 tttttcgtag gcaccttcag atttcaattt ttccatcaat tctggaatcg ctttatctgg
    31621 gtctacagta ccagtgttga tagctgtatc aaattgttgc attgtgttag caatagctga
    31681 gatttcagat ttcacattgt cagtattgaa gataaatcca agcgctggag attctttagc
    31741 ttctgccaat tctttcttag aattttcgat ttgttggtct gtaacgtttt cgttgatgta
    31801 aaggatccag ttgttaccag tgttccatcc acccatgtga gtgtttcctt tgtagccatc
    31861 aagaacgcga acacggtttt ctttaccttc aattttttcc cagttcttgc cttctggacc
    31921 gtaaacaaga ccgttcaaga gttctggatt cgtattcaag aggttcaaga tttccattga
    31981 tttttctttg ttcttagagt tgtttgagat gacaaagtta gcaacttgtg ttgtttggtt
    32041 tttcttgatg aagttagtaa ttggtttgat ttggatatct ttgttggcaa cacgtgaaag
    32101 caagctgtta ccgtagtcag ctggtcctac tgtttcttca cgaacgaacc aagtatcttg
    32161 ttgaaggtca aaggaagtat cgcttgttgc gacatctttt ggaatgtagc cagcttcata
    32221 gaatttgtga agagtcttca agtgttcttt gaaacgaggc acttcgtaac ggtttacaac
    32281 tttagtagta tcgccttcaa ggtcaataac gaatggaagc ccgtttgcta ctgggtagtc
    32341 aaagttatca gatgggatga aaactttacc aatagcaaat ggtactacgt ctggagcttt
    32401 ttctttaatt tgtttcaaga ctggctcaag agtttcgtaa gaagtaacac ctgaaatatc
    32461 gataccatat ttggcaagga gagttccgtt gaaggcaaag ttttgagatg atgcaacgtt
    32521 ggctgcaact ggaacagcgt aaattttacc gtttacagta ttacccttga tgtaagctgg
    32581 gtcaagtgcc ttgtaaaggt ctttaccttc ttttttgtac aattctgtca agtcagcata
    32641 agcacctttt tgagcattta caatatagtt atctgcaaag gcgatatcat agttttcacc
    32701 agatgatgtg ataactgaca ttttcttacc atagtcaccc cagccaaggt attggatatc
    32761 caatttagca ccaacttttt cttcgatgat tttgttggca tttgctaaca attcatccaa
    32821 gttatctggt ttgtcaccga tttggtacat tttgataaca ggtttgtcac ctgaatcagc
    32881 agcttttttg ctgttacctg tcaagttgcc acaagcagca agacctgcag ccagagcgac
    32941 tacactagca gatgcaaaag catatttttt ccagtttttc atgataaaaa ctcctttttt
    33001 tatttttaaa cttataaaca atgtaatgat cttatactca atgaaaatca aagagcaaac
    33061 tagaaaacta gccgcaggct gctcaaagca ctgttttgag gttgtagata agactgacga
    33121 agtcagttac atatatctac ggcaaggcga cgttgacgcg gtttgaattt gattttcgaa
    33181 gagtattaac ttcacacaag ggaagttggg aactgagaaa tgttcttttt caataagcac
    33241 tattctttca caccaccgat agtcaaacct tttacaaagt agcgttggaa gaatggatat
    33301 aaaatcgcga ttggaagggt tgcaaccaca accatggcca tacgacctgt ttctttcggt
    33361 agagcaactc ccagttgacc agtcaagccg accgctttgg caatgtagtc catattttgt
    33421 tggatttgca tgagcaaata ttgcaatgga tacaagttat cactcttgat gtaaagaagg
    33481 gcgttgaacc agtcattcca gaaaccaaga gctgtcaaaa gcgtgatggt tgcgatacct
    33541 ggtagtgaca atggcaaaca gatttggaag aaaatccggg cctcactggc accatcgata
    33601 cgagccgatt cgagaatggc ttctggaatg gtcttcttga agaaggaacg catcaagatg
    33661 atattgaatg gtgagagaag cattggaaca atcaaggccc aaacagtgtc cccaagctga
    33721 agtacacggg tcaccatgat ataacctggt accaaaccag cgttgaacaa catactgaga
    33781 aggacgaaga tggtaaaaaa tctgcgatac ttaaaggttg tccgtgaaat agcgtaggca
    33841 taggttgttg tgataaagac atttgtcaat gtcccaacta cggttacaaa gacagagatg
    33901 aagagggctt gtaggatttt atccttaaac tgtgccaaaa actcaaaacc gtctaagcca
    33961 aattgggatg ggaagaagct atagccgtat tggaggaggc ttttctcgtc tgtcactgaa
    34021 ataatgataa cgaatacaaa aggtaggata caagagaggg caatcaaacc cgaaatgata
    34081 ctgaagaaga tatctgcttt cttactgaag gagtgaatgc cgacattatc aattttttct
    34141 tttttaattt tcttttttgc catattctcc tcctttctag aacaaagctg agtttggatc
    34201 gactcgtctt gcaagcaagt ttgataggat aaccagaatc aaaccaacaa cggattggta
    34261 aagaccggct gctgcagcca taccgatatc tgctgtctga gtcaaaccat taaagacata
    34321 tacgtccaaa acgttggtta cattgtaaag ctgaccagca ttgtgtggga tttgatagaa
    34381 gagaccgaag tctgcgcgga agatatttcc gactgcaagg atggtcaata cagttacaag
    34441 cggagtcaac tgaggaatgg ttacgttgcg aatacgttgc cacttgctag ctccgtccac
    34501 tgtcgctgct tcgtagtagg ttggatcaat tcccatgatc gtcgcatagt acatgacact
    34561 gctatatcca aatcctttcc aaatacctag gaaaagtagg agatagggcc agatgcccag
    34621 gtcagcgtag aaattgactt ctttgagacc aagactttcc aatagatgat tgaacacccc
    34681 tttatcaata tttaggaagg catctgtaaa gaaactgatg ataacccaag acaagaagta
    34741 agggaacaac atagaagttt gaaaaatctt caccattctc ttagaacgga gctcgctgag
    34801 gataatggcg atccctacag atacaactaa acctagaaag ataaagccaa gattgtagag
    34861 gacagtattt cgtgtgataa taaaggcgtc tcttgaacta aataagaatc taaaattatc
    34921 gagtccgacc catttactat ttatgatact atctatgaaa ccattactgg tcatgtggta
    34981 gtctttgaag gcaaccacgt tcccaaatac tggaatgtaa aagaatagaa tcaaccagag
    35041 tgcccctggc aaaaccatca agagaaagat ccagttgtct ctcaatgttt ttgaaaactt
    35101 tttcataatt tcctcccttt ttattttgat atccatctaa aaattctttt ttagactttt
    35161 gataacgatt acattattag tatactccta tttgcaggtt aggttaaact cctaattata
    35221 gaaaaaactc cacaaattat gtagcagatt taaaacttta tcaccactat caaacaaatg
    35281 tcctaaatca attgtttatt ttatctctat tagcccagtg atggcgtcac tctgttataa
    35341 gcatccaaca acggggtata ctgaaaaatc tccagactag ggaactcagc gatagttcct
    35401 aatctggaga tttttaatat gttattaggc gtttgctttc aacttagcaa taacctcttt
    35461 aagattatca atcaactctg ctgcagtatg ctcagagcct ttttcatctg ccaagaacaa
    35521 aactgctttt tgaagttctt tttgagagtt ttcaaggaca tccttatcta ctgtttcaag
    35581 gtttgagtct ttaagaagtt tacttaattc cttggctaat ttcttgagtt tgatttgcag
    35641 actcatcttc tcctgctgtt tctttgcccg ctgtttgtcc tccatcctta gttgctgact
    35701 ggctttcctt aatggactct agggaagcaa tggcatcttt gactgtttgc aagatatcac
    35761 gtaaaccttg ctctgtcaaa ctatcatctg taagagcttt cttagcctct gccaaaacca
    35821 gacgtgctga atctgtggta ggattcgata cacctgtcaa tgatctcaaa agattttcta
    35881 aggtttgagt ctgcttacta atactagact aaaatcaaaa agcattacat aacagtgata
    35941 t
//
LOCUS       DBEJAQ010000018        35526 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.018, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000018 DBEJAQ010000000
VERSION     DBEJAQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.018"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(14..310)
                     /locus_tag="ACL297_000947"
     CDS             complement(14..310)
                     /locus_tag="ACL297_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071229.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
     gene            complement(294..2453)
                     /gene="pfbA"
                     /locus_tag="ACL297_000948"
     CDS             complement(294..2453)
                     /gene="pfbA"
                     /locus_tag="ACL297_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HGR0071230.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3584..4810)
                     /locus_tag="ACL297_000949"
     CDS             complement(3584..4810)
                     /locus_tag="ACL297_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGR0071231.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4853..5545)
                     /locus_tag="ACL297_000950"
     CDS             complement(4853..5545)
                     /locus_tag="ACL297_000950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGR0071232.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5718..7469)
                     /locus_tag="ACL297_000951"
     CDS             complement(5718..7469)
                     /locus_tag="ACL297_000951"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071233.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7459..9204)
                     /locus_tag="ACL297_000952"
     CDS             complement(7459..9204)
                     /locus_tag="ACL297_000952"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071234.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9332..9862)
                     /locus_tag="ACL297_000953"
     CDS             complement(9332..9862)
                     /locus_tag="ACL297_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGR0071235.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            complement(9859..10315)
                     /locus_tag="ACL297_000954"
                     /pseudo
     CDS             complement(9859..10315)
                     /locus_tag="ACL297_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10449..11276
                     /locus_tag="ACL297_000955"
     CDS             10449..11276
                     /locus_tag="ACL297_000955"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGR0071236.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11288..11383
                     /locus_tag="ACL297_000956"
                     /pseudo
     CDS             11288..11383
                     /locus_tag="ACL297_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
     gene            11431..11670
                     /locus_tag="ACL297_000957"
     CDS             11431..11670
                     /locus_tag="ACL297_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HGR0071237.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12053..12148
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12548..13129
                     /locus_tag="ACL297_000958"
     CDS             12548..13129
                     /locus_tag="ACL297_000958"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGR0071238.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13129..14391
                     /locus_tag="ACL297_000959"
     CDS             13129..14391
                     /locus_tag="ACL297_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGR0071239.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGNGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14442..14903)
                     /locus_tag="ACL297_000960"
     CDS             complement(14442..14903)
                     /locus_tag="ACL297_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HGR0071240.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(14900..15664)
                     /locus_tag="ACL297_000961"
     CDS             complement(14900..15664)
                     /locus_tag="ACL297_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HGR0071241.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(15845..16249)
                     /locus_tag="ACL297_000962"
     CDS             complement(15845..16249)
                     /locus_tag="ACL297_000962"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGR0071242.1"
                     /translation="MKDFHFDAISAFESYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(16319..17800)
                     /locus_tag="ACL297_000963"
     CDS             complement(16319..17800)
                     /locus_tag="ACL297_000963"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGR0071243.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(17819..18997)
                     /locus_tag="ACL297_000964"
     CDS             complement(17819..18997)
                     /locus_tag="ACL297_000964"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGR0071244.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19162..20169
                     /gene="galR"
                     /locus_tag="ACL297_000965"
     CDS             19162..20169
                     /gene="galR"
                     /locus_tag="ACL297_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HGR0071245.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20216..21253)
                     /locus_tag="ACL297_000966"
     CDS             complement(20216..21253)
                     /locus_tag="ACL297_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGR0071246.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(21254..21607)
                     /gene="nmlR"
                     /locus_tag="ACL297_000967"
     CDS             complement(21254..21607)
                     /gene="nmlR"
                     /locus_tag="ACL297_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HGR0071247.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(21851..22741)
                     /gene="czcD"
                     /locus_tag="ACL297_000968"
     CDS             complement(21851..22741)
                     /gene="czcD"
                     /locus_tag="ACL297_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter
                     CzcD"
                     /protein_id="HGR0071248.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            22874..23410
                     /gene="sczA"
                     /locus_tag="ACL297_000969"
     CDS             22874..23410
                     /gene="sczA"
                     /locus_tag="ACL297_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator SczA"
                     /protein_id="HGR0071249.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            complement(23576..24382)
                     /gene="pnuC"
                     /locus_tag="ACL297_000970"
     CDS             complement(23576..24382)
                     /gene="pnuC"
                     /locus_tag="ACL297_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGR0071250.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(24727..26247)
                     /locus_tag="ACL297_000971"
     CDS             complement(24727..26247)
                     /locus_tag="ACL297_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HGR0071251.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(26240..26968)
                     /locus_tag="ACL297_000972"
     CDS             complement(26240..26968)
                     /locus_tag="ACL297_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0071252.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(26983..27747)
                     /locus_tag="ACL297_000973"
     CDS             complement(26983..27747)
                     /locus_tag="ACL297_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGR0071253.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            complement(27751..28194)
                     /locus_tag="ACL297_000974"
     CDS             complement(27751..28194)
                     /locus_tag="ACL297_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGR0071254.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            complement(28876..29172)
                     /locus_tag="ACL297_000975"
     CDS             complement(28876..29172)
                     /locus_tag="ACL297_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGR0071255.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(29194..30258)
                     /gene="pepA"
                     /locus_tag="ACL297_000976"
     CDS             complement(29194..30258)
                     /gene="pepA"
                     /locus_tag="ACL297_000976"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HGR0071256.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            30659..31360
                     /gene="galE"
                     /locus_tag="ACL297_000977"
     CDS             30659..31360
                     /gene="galE"
                     /locus_tag="ACL297_000977"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGR0071257.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            31376..31486
                     /locus_tag="ACL297_000978"
     CDS             31376..31486
                     /locus_tag="ACL297_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HGR0071258.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            31783..32742
                     /locus_tag="ACL297_000979"
     CDS             31783..32742
                     /locus_tag="ACL297_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071259.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            32732..33688
                     /locus_tag="ACL297_000980"
     CDS             32732..33688
                     /locus_tag="ACL297_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGR0071260.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            33685..34437
                     /locus_tag="ACL297_000981"
     CDS             33685..34437
                     /locus_tag="ACL297_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071261.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            34533..35498
                     /locus_tag="ACL297_000982"
     CDS             34533..35498
                     /locus_tag="ACL297_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0071262.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
BASE COUNT        10460 a         8034 c         6484 g        10548 t
ORIGIN      
        1 atttgtccta ttattatttc attttattat actctgttaa ttcatatgag tttaaaccga
       61 tttcatcttt aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt
      121 tacaattaac cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta
      181 tctcctcttt agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt
      241 gtgaacctac aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta
      301 tctttaccat aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt
      361 aagaatagtt ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact
      421 atttctttag atttattaat atgattttca gtttctctgc catctccaac tattttatag
      481 tttacttctt ctgtcttatt atcttgttta ttgtcgatct tgtcattcat ttgtctatta
      541 tctttacttg agttaaactc tccgttcttc tggttactat caattacatt atttgaatta
      601 gattgttttt cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca
      661 attttgtaaa gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca
      721 ggttctttcc cgtttgcatt agatttgatg aatgatatat acttaccgga taaattataa
      781 aattggttat ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta
      841 ccaccgaata tactattctc tgacactact aaattatcac taactcgtaa taattcaata
      901 tttggttgtt ctgtttcctt agaattatta ttaattacat tttttgttac agtaatatct
      961 gatacttttc ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga
     1021 tcggcaatat taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct
     1081 ttagtgtttt tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca
     1141 ctctctcctt taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct
     1201 gtaaaacgta cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta
     1261 gaggggttct gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca
     1321 gatttatcac ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca
     1381 tcatcattca aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc
     1441 ttacttatga tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa
     1501 ttatcaacta atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc
     1561 ttgaatgtta cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa
     1621 gaatttataa gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata
     1681 tcaatcgtac caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca
     1741 tcatccgtaa ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca
     1801 tttagaattg tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt
     1861 tcttttacta aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct
     1921 gcagcatcta ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca
     1981 ccataatctt ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt
     2041 ataacatctt ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt
     2101 ctgttgtcta attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt
     2161 aacgaattat ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc
     2221 tgattaacag ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta
     2281 gccatcggtg aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt
     2341 gaaattgcca atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca
     2401 ttaggaacaa aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta
     2461 ttacttttct taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc
     2521 aaattgaatt gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct
     2581 cttcaaacta ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta
     2641 tagtagactg aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt
     2701 aactgtcccg atcaatttgt cctattcttg tttcatttta ctatatctac aacctcaaag
     2761 cagggttttg agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa
     2821 gtgatgctat ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt
     2881 gatatgttgt aaattataaa tgaaagaaca aataccttgt catacaagca ttcgccaggg
     2941 atatttacct gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct
     3001 taaatagacc ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta
     3061 aaatactgga atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcag
     3121 gagatctaat gatgtatagc aagcatcact aaacatatgg gatatttgcg ttattataat
     3181 atcaacatgc tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca
     3241 aaactttgaa caatattctg aaaacaaaaa aaagacgcta gtttccacaa tctcactcat
     3301 ggaaactcac gtctttcaaa aaatagggtg aattgaaaac tagaacaaga cactagattt
     3361 tctaaatgat gtctagtaag gattctaaat gctataccaa attgctcaca tcttatttca
     3421 aaccacggga ctcccacaag aaaggaaaat cctataaaaa ttctctctct gaggagaaag
     3481 aggcctcaac tacaggtgta aactattatc aattgaaaac aacagttttt acactcaatt
     3541 agacttttta gggacaggat cactttcgct tgactgtagt ttgtcatttt tttgataaag
     3601 ttagcacttt ttcagaaact gttttgaaag tctcaatgat atagtctact tcttcatcgc
     3661 ttaatttagt atgaagaggg agggtaattt cattctcaaa gaaggcatag gccttaggat
     3721 agttcgtcat atcaaatcca agattcttat aggctgtcaa gagaggaagc ggtttgtagt
     3781 gaacattact tgcaattcct gctttagcca attcttggat gatgaggttg cgttcttcta
     3841 ggcttgctcc ttctacacgg gtgatgtaga ggtggcgtga agattcgaca gtttcagtct
     3901 tgtgtgccaa aggatggatg cgagaacctg caaaaccact atcatagcgg tccacaatgt
     3961 ccttacggcg ttgcaacaaa cttggatagc ggtccaattg taccaaacca agtgaagcca
     4021 tgatatcggt catgttgcac ttataggctg gtgtaacgat atcgtattcc catgacccca
     4081 gttgcatctt ggcaagagca tccttagttt gcccgtgaag ggaaaggatt tggaattcct
     4141 tgtacatctc ttcgtcatca atcactggat tggctttcca agtcgcactt ccaccttctg
     4201 ccgttgtaaa gttcttaaca gcatggaatg agaaggaagt aaaatcagcg atagaaccag
     4261 aaggttgtcc tttataagta gatcccaaag cgtgggcact atcagagaca atgacaatac
     4321 ggttaaaggc cttttgccac ttgcttgaag cggtaaagaa gtcacgtttt ttctccacga
     4381 cttggaacaa acggtcataa tcgcaaacaa tccctgcgag ctctactggg ataatcacct
     4441 tagttttctc agtgatagct tgctcaagca ggtcatagtc catctcaaac gtatctgctt
     4501 ggatatccac catgacaggg gttgctccca cgtgcgtaat gacactacat gaagccgtat
     4561 aggtcatggc tggaacgatg acttcatcac caggtcccac ttccaaaacg cgtaaaatca
     4621 actccagagc ggctgtcgca gagttgagac aaacagtctt aggtgtctgt gtgtaaagag
     4681 acaagcggcg ctccagttct tttgttttag gacctgttgt gatccaacca gaacgcaggg
     4741 tatccgctac ttcagcaatt tctgcttctg tgatatcagg cggtgaaaat ggaatattgt
     4801 aatttggcat tgatttgctc cttttatctg tactcatttt cttatgacta ctttatttta
     4861 gtacctcaaa cacggtttga aacatgattt tgatgtcccc aaagaaacta aactctcgga
     4921 gataggcgag gttatagcgc atcttttcag gaagaacatg ctccacatag gcctgatcaa
     4981 ctgacagacc tttctccgtc atttgactga tgatggtgtc ctcatccttg tagttgatgc
     5041 tggctggaga ggtaattcct gcttgcaaga gcaaggttgc catcatttca gggctatact
     5101 gctctgtata acgtggcact tcaggtcgtg taccgacaaa ggacatctca cctttaagga
     5161 cattgaccaa ctgaggcagt tcgtccaaac ggacacgtcg gatgaaattt ccaaccttgg
     5221 taatacggct atcgttagca gaagtcacca gacttccttt tttatccgca tccgtcacca
     5281 tggtacggaa cttccaaatc ttgaaccgac ggttgtactg ggtcacacgc tcttgcttgt
     5341 aaatcactgg ccccttgcta tccaacttga tccaaatgct caagatgaga aagatgggag
     5401 aagttagaac cagtaagacc aaggccaaaa cccagtccaa gcaacgcttg aaaatcagcg
     5461 aacccttcct tttagagaca agctggtagt aagactcaac ctcgcttgat ttcatttcca
     5521 caggcaagtc ttcccatttc agcatgctgt tctcctttgt ttttattata ctatttgtca
     5581 acatttttca ttataccaca aaatggaaaa ggcggtgaaa gaaatctgta atttgaagga
     5641 tttccttcta gtactcaatg aaaatcaaag tacagctttg agattgcaga taaagctgac
     5701 atggtttaaa gagattttca aagagtatag gccatggccc ctgcctgcaa actatacatc
     5761 ttgtgatagg ttcctcccag agtcaagagt tcctcatggg ttccactctc gataatgcgt
     5821 cccttatcca agacatagat gcagttggcg tcttgaatag tagaaaggcg gtgagcgata
     5881 gcaatagttg ttcggccctg tctcatcttc gccagagaag cttgaaccaa actttctgtt
     5941 tcagagtcaa tattggctgt cgcttcatcc aaaatcagga ttttaggctg gctggcgact
     6001 gttctagcaa aggcaagaag ctggcgctgc ccagtagaga agctcgaacc acgctcggaa
     6061 acaggggagt cgtacccctg aggaagttct tgaataaagg aatctgcatc cacaaaggct
     6121 gccgcagcct gaacctgctc atcactgatt tcttggtaca tggcgatatt ggacttaata
     6181 gttccatgat agaggaaggg ttcctgtaaa accaaaccga tgttttttct cagctcttct
     6241 tgactgaaat ccctgatatc cacatcatcc aagagaactc tccctgactg gaattcataa
     6301 aagcgcatga ggacattgat aatagacgat ttccctgaac ctgtatgacc tacaaaggca
     6361 atggtttcac ccttattaac agagaaagaa atgtcatcca gaatcggatg tttaccgtca
     6421 tacgagaaac acacatgttc aaaacggata ttgccttctt tgactttggc ttgcccattt
     6481 tcttgaaggg gctcataggt cctctcatcg attaaggcaa agacacggcc tgcagaaacc
     6541 atagacgttt gcagtgttga aaagttttgc gtcacctcaa tcaaagggtc aaagaggcgg
     6601 ttgatgtact ggataaaagc atacatggtc ccggccgtta tcccgataga aagaccacga
     6661 tagccaaaat aggtcatcaa gacagcataa cctagaagtt tcaacaaact catggcaggt
     6721 ctcaaaaaga gggcatccaa ggctacagaa cggttggcat aggccaagtg ctcttggttg
     6781 atttcatcaa attctgcctg caggcgcttc tcttgattaa aagcctgaat aatcctgatt
     6841 ccctcgatat tctctgccag cttactattg atatctgaca agagacttct ggttttctcg
     6901 atgattttca ctgacttttt tcgatagaga ttgaccaaaa ggaaaatcaa ggggagaaag
     6961 agcaagacta aagccgtcaa acgaaaatcc agcaccaaca tggtataaag ggttgtcaga
     7021 aagataaaaa ctgctgagat aaagctggat aaaatcccag aaaacatatc actaatcgtc
     7081 tcggtatcgt tggtcaaacg agaaacgata gaacctgctg gcgtcttgtc aaagtaagac
     7141 atgcccagtt tctccatatt ggcaaaggca tcccgacgaa tatccctaac aatactgtaa
     7201 gacacgcgcg caaagagaag attgccgaca tactgaacta cagtttgtag gatgtagaga
     7261 ccatagtaga ccagcaaaac ggtcacggct agttggttaa gattgctgag atactggtcg
     7321 ataaagtggg aagccacaag gggaatgaca cttttaatga ccgtcgtcgc taggagaaaa
     7381 ctgagtgcca aaaaggtaag gagtccataa ggcttgagat aggacatcaa gcgcttcaat
     7441 acagtccatt gttcttgttt attctgcatc ttcttctccc ttcatttcca gctgctgaga
     7501 ctggtaggtt tgggcatacc agccatccaa agctagcaag tcttcgtgcc tgcctcgttc
     7561 gataatttga ccattttgca ggactaaaat caaatctgca tggacaactg cactcaggcg
     7621 atgggcagtg ataatggttg tcttgtcctt tcgcgtctcc ttgaggttgt cgataatcgc
     7681 atactctgtc ttggcatcta cggcggataa ggaatcatcc aaaatcaaga tatcagggtc
     7741 taaaatcata gcccgactca tagccaaccg ttgcttttga ccaccagaaa gactgactcc
     7801 tttttcacca atcagcgtat caaatccttg aggcatgtct acaatatctt ggtaaactcg
     7861 ggctagctta gtagcttcct cgaccgctga aaggggcaag ttaggattgc caaagcggat
     7921 attgtctagg attgaagtcg caaaaagaaa ctggtcctga ggaacatagc ccatgagact
     7981 gcgaaggtct gtcagacgat agtcccgaat atcgtgaccg tttagataaa tggcgccctt
     8041 atccacatcg tattcacgca agaggagctt gattaaggac gttttcccag agcctgtctg
     8101 cccaaccaag cccagtgttt gcccttttgc caaactaaag tgaatatccg tcagtgtttc
     8161 ctcattttca aaagcaaagc tgtcaatggc atactccaaa cgcccatttt caataccatc
     8221 cagaggaaac tccgggtctt gtacaggaga ttcctgagac aaaagatttt caatccgctg
     8281 gtaagaaacc ttccctcgct gagtagtatt aaagaggaag ccgatggcca taagaggcca
     8341 gaccagcata tccaaatagc tgataaaggt gactagattc ccaactgtaa tctgcccttc
     8401 ctgaaccacc aaggagccaa ccaaaagcgt taaaacatag gacgaaccaa caaacaagag
     8461 aaccataggg tcaaagagac tatcatattt catggtttgc aggttctttt ggaaggttaa
     8521 ttcattgact gcctgaaaag acttcaactc gtctgcctga taaccgaaag acttggtcac
     8581 tttgatacct gatacggact cctgtacctt gttattgagt tcagaaaaag cagcttggga
     8641 ttcgccaaag gccttatgag tctttctccc taggcgacta gtcgcatagg ccatgaaagg
     8701 tagggggaga atggcaacaa gagtcatctg ccatgagatg ctaaagagca tggtcaacaa
     8761 agtcaccaga gccgtgatag aggcatccac cgcagacatg acaccgccac ctgctaaacg
     8821 agtcaaggca ttgatatcat tggttgcgtg tgccatcaga tcacccgtcc gataggtttg
     8881 ataaaaggct gacgacattt ttgtgaaatg cttaaacaag cgagaccgca tgatctgtcc
     8941 caagcaataa gaggtcccaa ggatatacat acgccacaca tagcgcaaat agtacatacc
     9001 aaaggctgca agtagcaagt aaaataggct aagaaggagg tcctgctggg ttaattgccc
     9061 cgatgtgatg gcatcaataa cccgccccat aaccatagga ggaatgagat tgaggacgga
     9121 aaccaagacc agggccacaa ttccgactag ataacggcgt ttttctaact tgaaaaacca
     9181 ccaaagtttt tgaataatgg acataaaatc cctttctgat tgcaaatgga aacctgaggc
     9241 caatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggctg ctcaaagcac
     9301 tgctttgagg ttgtagatag aactgacgaa gtcagtaacc tacatacggc aaggcgacgt
     9361 tgacgcggtt tgaagagatt ttcgaagagt acaagacccc aggtttttct ttttcataag
     9421 ttacgactgt aacagcaccc ttgtcatatt cagcaataaa gatattggct acattgtcat
     9481 gcccttgttt actgaggtta tcaagcaacc actcttcgct acgaccaatc gattccaaga
     9541 catctacttg aatcacaccg tcagtcacaa ctggatactt aggattttca tctcccattt
     9601 gcacaacgat aagttgccca ttttgctctt gcacagctcg tttgacttgc ttcatctgga
     9661 aaatcccttg gctacgaagt ttgagggcta catccgatgc agacaaacca actgaacgac
     9721 aggcttctgg gtcaatattc ccatttttga taaggagagt tggcttacca tcaatcaaac
     9781 gtttcacaaa gtgaacattg ttgttgagcc atttgagagt caagaccagt ttgattaaaa
     9841 tttcgatata attaagtgtc atttgttcac atccaccagt tcaattgcgt ctgtcttata
     9901 caattcgact ttctctaaca ggtacttgtc tggctcactt ccgtttaggg cacgataata
     9961 gcgatctccc accttgataa gtgcgccatc tgtgtttgtg gaagtattaa catagacttc
    10021 tgacttgtct actcccaaat ctttggaaac aacctcgatg aaatgaagtg aagtttgaaa
    10081 tttattgtta gaaacttgat tgttttgata agtcgtgatg ctgaccaaaa gcagagctaa
    10141 ataaggccag agctgaaatc atgaccaact cacgaaattc ggttcccttt ttatcatggt
    10201 atgccttgaa agcaaaaaaa actgtcacag ctaatagaac aatcccaaag ccaaccatga
    10261 taccattttg ctgaccgatt tggctgagta catagtcata agagtagaat ttcatatatt
    10321 ttcactccct ttcataaaat cattcttaat tataaacgaa agagagtaat ttttcaaacc
    10381 atacgttggg gaaatagatc tttttttggt ataataaaat ctataatctg aatgaaaaag
    10441 gtaactttat gaaacttatc tcatggaata ttgattccct aaacgctgcc ctaactagtg
    10501 actcagctcg tgccaaattg tcccaagaag tcctacaaac cttggtcgct gaaaatgctg
    10561 atatcattgc tatccaagaa accaagcttt ctgccaaagg acctacaaag aaacacgtgg
    10621 aaattttaga agaactcttc ccaggctacg aaaacacatg gcgctcttcc caagagcctg
    10681 cccgtaaagg ctatgctgga accatgttcc tttataagaa agaacttacg cctacgatca
    10741 gttttccaga aatcggtgcc ccttctacca tggacttgga aggccgtatc atcactctag
    10801 aatttgatgc atttttcgta acccaagttt acactccaaa cgctggtgac ggtctcaaac
    10861 gcttggaaga acgccaagtc tgggatgtta aatatgctga gtatttggct gaactagaca
    10921 aagaaaaacc agtccttgcg accggtgact acaacgtagc ccacaatgaa atcgaccttg
    10981 caaatcctgc tagcaaccgc cgttcacctg gatttactga cgaggaacgt gctggattta
    11041 ccaacctttt ggcaactgga tttacagaca ccttccgcca tgttcatggc gatgttcctg
    11101 aacgctacac ttggtgggca caacgcagca aaacttctaa aatcaacaat acaggctgga
    11161 gaatcgacta ctggctcaca agtaaccgta tcgctgacaa ggtcactaaa tctgatatga
    11221 ttgactcagg tgctcgccaa gaccatacac cgattgtctt ggaaattgac ttgtaaggat
    11281 tttctatatg gactacaatg cggtcattcc cgagtttctt gtatcgaata tcgaacagtc
    11341 ccggtccttc tactgtggtt tactgggttt taggattgaa tactaacgtc cagaagaaaa
    11401 ctttctcttt ctcttgaaga gtgtcaacta atgttagagg agggaacaaa ggatcaacta
    11461 gctgaactga cctacccctt tggtcgtgga gtcaatctct cctttggcat aaaggatgtt
    11521 ccaaaactct atcaaaaagt gatggaggca aactatccta tttatcgtct cttgaccaaa
    11581 agaaagtttc gtgtcagtga tccctatatc tatcctcata aatttgcagt tttggatcca
    11641 gatggctatt ttttaagatt tagcgagtag aataataaaa attgggaaaa gtctttaaag
    11701 ttagaaaaag gcatattgtc aggtgtctaa aaacttgata gtatgccttt tatgatggaa
    11761 aaatcttcta atactcttcg aaaatctctt caagccacgt caacgtcgcc ttaccgtagg
    11821 tatatgatac tgactctgtc aattctatcc acaacctcaa aacagtgttt tgaggttgtg
    11881 gatagcttcc tagtttgctc tttgattttc atttagtata aatgttagct tacaatggag
    11941 tttttgttca ccctctctta tttttgttcg ctttttaaac caaaacacta acaaaatagt
    12001 tgatttctaa agttatttag tgtaaaataa aaacattggc tcaagcctat ttaaaattga
    12061 atatcgtttt acttgtttat gtcgtgaatt ggcacgacgt ttctacaagg tgccggaaca
    12121 cctaacaata agtaagtcag cagtgagata tggccgtttt tgccatgtct atttagggga
    12181 cttacttaga gattaagtag gtcttttttg aatattttta atatttccct taattagtgc
    12241 ggacgggagg tgaaattatc ctgtgaataa tttcaggaat ccagggaatt tcatccctaa
    12301 tttttgagcc cctagctctt tcatttctag gcttaggcta aaatagttcc ccgaactatt
    12361 ttactctcaa aaatcccgtc atagacaata acaagattat tgtctatgat accactacaa
    12421 cgggaaatat ttgataagac tcgcttcgct cgtaatagtt ttatcaaata tatagtatta
    12481 gcaaaaggga tcactataga aatatttaaa caacaagtaa attaactttt agactttagg
    12541 aggtcttatg aaattattag aagagcgcat cctcaaggat gggcatatct tgggtgataa
    12601 catcctcaag gtagattcct ttttaaccca ccaagttgac tttagcttga tgcgagagat
    12661 tggtaaggtt tttgcggaaa aatttgctgc tactggcatt accaaggtcg taaccattga
    12721 agcgtcgggt attgccccag ccgtttttac agctgaagcc ttaaacgttc ccatgatttt
    12781 cgccaaaaaa gctaagaaca tcaccatgaa cgaaggcatc ttaactgctc aagtctactc
    12841 ctttaccaag caggtgacca gcactgtttc tatcgctgga aaattcctct caccagagga
    12901 caaggttttg attatcgacg atttccttgc taatggccaa gctgctaaag gcttgattca
    12961 aatcatcgaa caggccggtg ccacagtcca agctatcggt atcgtgattg agaaatcctt
    13021 ccaagatggt cgtgatttgc ttgaaaaagc aggctaccct gtcctatcac ttgctcgctt
    13081 ggatcgtttt gaaaatggtc aggtcgtatt taaggaggca gatctctaat gcaaactcaa
    13141 gaaaaacact cgcaagcagc cgttctgggc ttgcagcact tactagccat gtactcagga
    13201 tctatcctgg ttcccatcat gattgcgaca gcccttggct attcagctga gcagttgacc
    13261 tacctgattt ctacagatat cttcatgtgt ggggtggcaa ccttcctcca actccaactc
    13321 aacaaatact ttgggattgg actcccagtc gttcttggag ttgcattcca gtcggtcgct
    13381 cccttgatta tgattgggca aagccatggt aatggcgcta tgtttggtgc ccttatcgca
    13441 tctgggattt acgtggttct tgtttcaggc atcttctcaa aagtagccaa tctcttccca
    13501 tctatcgtaa caggatctgt tattaccacg attggtttaa ccttgatccc tgtcgctatt
    13561 ggaaatatgg gaaataacgt tccagagcca actggtcaaa gtctcttgct tgcagctatt
    13621 actgttctga ttatcctctt gatcaacatc tttaccaaag gatttatcaa gtctatctct
    13681 attttgattg gtctggttgt tggaactgcc attgctgcta ctatgggctt ggtggacttc
    13741 tctcctgttg cggtagctcc acttgtccat gtcccaactc cactctactt tgggatgcca
    13801 acctttgaaa tctcatctat tgtcatgatg tgtatcatcg caacggtgtc tatggttgag
    13861 tcaactggtg tttatctagc cttgtctgat atcacaaaag atccaatcga cagcacgcgc
    13921 cttcgcaacg gttaccgcgc agaaggtttg gcggtacttc tcggaggaat ctttaacacc
    13981 ttcccttaca ccggattttc acaaaacgtt ggtttggtta aattgtcagg catcaaaaaa
    14041 cgcctgccaa tctactacgc agctggtttc ctggttctcc ttggactgct tcctaagttt
    14101 ggtgcccttg cccaaatcat tccaagctcc gtcctcggcg gtgccatgct ggtgatgttt
    14161 ggttttgtat ctattcaagg gatgcaaatc ctcgcccgag ttgactttgc taacaatgaa
    14221 cacaacttcc ttatcgcagc tgtttcaatc gctgcaggtg tcggtctcaa caacagtaat
    14281 ctctttgtca gcatgccgac agccttccaa atgttcttct caaacggaat cgtcgtagcc
    14341 agcctactcg ctattgtcct caatgccgta ttaaatcata aaaagaaata agaaaaagag
    14401 gtgcgagcct cttttttgtg tgacaaaata aaaacatcta cctattcata tatgctagtg
    14461 tcatagtcct gaaaatcatc tgcatcaaga acaatttcac aactcttata cttttctctt
    14521 acaagtcgtt cggcttcatc tggattttca gcctctatac ttactaaacg tgataaagtt
    14581 tctgtaattt ctactgtata ctttttcagt tttagattgc tttccaataa ttcacctatt
    14641 aacaataatc tatgatagga gctatgtagc cactctggca atttatctcc tacttcccac
    14701 ccatattttt ccttcatcaa agctagttgt ttttcgtcac caaatttcat attttcagat
    14761 aaccatctca cacaatcggg atctgataag gtacagacga ttaaacttag gttatcgtaa
    14821 cggctttcag tttcataatc aggccagatg acttttaaga aatcatacac ctcttctgat
    14881 gcaacaagtt gtttcgtttt cataatttcc gatactttcc tctaccttta aattctatta
    14941 tttctttgtc tcttaatatt tgaagctgtt gcctaatctt ttctttgata tgattgttct
    15001 taacaaagat attttttagg tcactttcaa aacgatacat atcttcaagg gtaaattctg
    15061 aaccctctat cttatctata caatttagaa tttctattgt ccaacctctt gatgacagag
    15121 agctcttcct taaaaataaa ccttgcttaa attcttttgt aactttttct ggatctctaa
    15181 cttgtccatc ttgcacaaga aatatccttc ctttagaagg tacttgtgat aaatcaatgt
    15241 tacaaccaat ccaacctgct cgtctagcag ttggtgcaag tggttttctt tgaataatcg
    15301 atttcggtgt aacaaattgc ttcggaagga caagaaagtt atttacctca aaattttttg
    15361 tgtaagttaa aaaaaagaaa ttaggattat tatctgcctg cacacgcttc atcatcgttg
    15421 cataagcacc atcattgatt gttgatgaaa aatttccttt tttgctcttt agttcaaact
    15481 cctcactaca atgattacag taaaaatctg ctacaggccg attattttca aaatgattta
    15541 agggattgtt cccacaatta gggcaataag actgacgata aacccaatcc tccgttaaga
    15601 tacgtgcctt ttggctatta cttttatagg tttctactaa ttctaaatta aagtgtaatt
    15661 ccatttaaaa attcctcact aatatgagaa ctcactcgat ataagcaatt aaaatctact
    15721 ataactagta taccacatat atgtacctat tacatttgta tttcatcact ctcttttata
    15781 tttccttcta acatatctca taaattaaaa atgagtgaga taaatttatc ccactcacct
    15841 ttctttatat acttacccgt ctttcctcag acctttggcc tgtgacaaac atggtaaagg
    15901 ttgccttaca gacatttctg ccctcttgat tggtgatatc cacatccatc acacaggttg
    15961 tacgaccttg atggacacat tctcctttaa tggtcaatac atcgtcgagt tttcctgcct
    16021 taaggtagtt gatagaggat tggagtgtca ccccatcaag tcccagcgag ataaccacca
    16081 aaccactgat ttggtcacat agcgtgaata gatagccacc atgggcattg ccatagtagt
    16141 tgagcgatga gttcactact ttggtcgtca ccacaacgtg accatctctc attttttcaa
    16201 tttcgtaact ttcaaaggca gatatagcgt caaaatgaaa gtctttcatc gtttcctcct
    16261 gatatttcaa acgacactat gatactactt tttataggac tgtgcaagga gaaagctcct
    16321 agtctagtaa aattccgacc tgttccacaa agcgcataaa ggctgtctgc ccttgttctg
    16381 tctgcttgta gactcctgca tcctcaagta cacgcacaaa gatagcaccc acagagtcct
    16441 tgacgattgc aagggctttt tctttatccg ttaggtctgg atgttgggat ttgagttggt
    16501 ctgcccactc ctgatgataa tcggcaactg taacagcttc tcctacaaga tagctagcga
    16561 cttgctctac ttcttctttc agacgtggtg gcaagattgc caagcccatg acctcaatca
    16621 agccgatatt ttccttcttg atatgttgga catccttgtg gggatgatag atgccatcag
    16681 gatgctctgc tgaagtctga ttgtctcgca agaccaagtc caactcaaac tgtccatcac
    16741 gcttacgcgc tattggtgtg atggtgtgat gcggtgtccc gtctgtctct gctaaaatct
    16801 gcacactaga atctgaatac tgacgccatt cctggaaaat cttatcagct aagttaatca
    16861 aatcctcttt ggaatccgaa gtcaagcgta ggacagacat tggccacttg ataattccag
    16921 ccttgacctg ctcaaaacca gcaaatcgga aggtcttttg caagggagcc aattccatag
    16981 gaaatacgtg acggcctccc tgataatgat catgagttag aatagagccc cccacaatcg
    17041 gcaggtcggc attagatcca gcaaaatatc ctggaaactg ctctacgata gccaacagac
    17101 gttcaaaact ctgacgacta atggccatgg gacgatgctg gccatctaaa aagatacaat
    17161 gctcattaaa gtaagcatag ggcgaatact ggaaccccca ttcctgacca accatttcaa
    17221 aacggataat ccggtggttg ctgcgagctg ggtggttgac ccgaccatgg taaccctcat
    17281 tctctaaaca aagttgacac tgaggataat tactagcttg caccaacttg gctaccgcaa
    17341 tctctttcgg atccttttca ggcttagaga gattgatggt aatttcaagt tctccataat
    17401 cagatggaac acgataagcg atatttttag caatggcctt gagtttgatg taatcatttt
    17461 tctgactgag ttgataaaaa tcctctatcg cttgctcagg agagtggaca taggcttccc
    17521 agaagtcacg attgacctga ctcggataag gagtcaccaa gtccatcagt tcagtaccaa
    17581 ggatttcacg cgcagtctga ctatcctcaa tcatctctaa tcgaacggct tcctcaacta
    17641 gctggtcctt gaggtcaatc aatttatcca gattggtctc aacttccaaa acaccttctc
    17701 ccactcgtgc caaaacacgg ttggtcaggt agattcgatc catttcctca aatgaacttt
    17761 cagaaatgac atgtgttaca aatttatcta ctaagatcac tatagagcct ccttttgact
    17821 agtcaaggac gcgagtgcca cctgcaactt cagcgatata gaagcttgga gcgtatccaa
    17881 ctacttcctc gtagtgtttg cctacagctt ccttaaaggc ctcaacagta tctttttgaa
    17941 ccaaggcaat ggcacagcca ccaaaaccag cccctgtcat acgagcaccg agaactcctt
    18001 cttgtgccca agctgtgtga acaagagtat ccaattccaa accagttact tcataatcat
    18061 gctccagaga aacgtgtgac gcattcatca aacgaccaaa tgtttccaaa tcacctgctt
    18121 gaagggctgc ttgagcttta agggtacgtt ggttttcaag cacagcatgg cgagcacgtt
    18181 tcaaacgatt ttcatcttta atcaaatagc tatattggtc aacggcccac tcgtccaatt
    18241 cacccagggt ctgaatatcc aaggcaactt gcaattcttc cactgctttt tcacactcag
    18301 cacgacgttc attgtattta gagtccgcca attcacggcg tttgttggtg ttcatgataa
    18361 caacgacatt gtccttcaaa tcaagtggca ccaagtcgta ttctaaagta ttggtatcta
    18421 agtaaatagc acgttggtca gcccccatac caatagcaaa ctggtccatg ataccagagt
    18481 tgactccgat aaagttgttt tctgtttgtt ttccgatttt aaccaaatcc aaacggtcta
    18541 gttttaaatc aaagagatgc tctgccacga ctcctgtcaa gagctccaag gatgctgaag
    18601 aagacaagcc agcaccattt gggatattcc cataaacata aaaatcaaaa cctttgtcaa
    18661 tcacgtgccc agcttcttgc aagaaatgaa gaaccccttt tggataattg gtccagttgt
    18721 gctctttttc aaacttgagg tcagcgagag gcacttcgat aatgcccttg tcctcaaagt
    18781 tagctgagta gaaacgcaag acttggtcgt cacgcttacg agctgcaccg taagttccca
    18841 aggaaatagc agcaggaaaa acgtgcccac cgttgtagtc tgtgtgttca ccaatcaaat
    18901 taatacggcc tggtgaaaag aaggtttggt ctgcttcttg accaaaaaca gcaagaaagt
    18961 ctttgcgaag gacttcagta gtaagatgtt gtgccatatg aattctcctt tgactgtccg
    19021 ttaagccatc ttaacgtgta atcgttttct ttctattgta tccaaggaat ttcccaaggt
    19081 caatcctttt tactaaaatt ttactaaaat atcagtaaaa agaagaaaaa tatgatatac
    19141 taacctaatc aaggaggatt tatggctacc ttaaaagaca ttgcacagct agcctctgtc
    19201 tcaatcgcga ccgtatcccg tgtcctcaac cgcgaccaga gcctatctgt tacagaagaa
    19261 accagacacc gtattttaac cgttgctgaa gagctgggct acaccaagca cctcaagaca
    19321 ggcgagtccc acaaacccaa gcaaaagatt gccattattc aatgggtcag cgaacaaggg
    19381 gagctagacg acctctacta ctaccagatt cgcctaggaa tagaaaaaag agcccaagag
    19441 ttggactacg atatcttgcg ctattttaat gaccaccctt ttaccctaag cgaggaagtg
    19501 attgggattc tctgcatcgg aaagtttagt cgagctcaga tttctgcctt tgaagaatac
    19561 caaaagcctc ttgtatttct agacagcgat acactttccc tgggacatac ctgtattatc
    19621 acggattttt acactgctat gaaacaggtt gtcgattatt tcctcagtca aggaatggac
    19681 cgtatcggga ttctaacagg ccttgaagaa acaacagacc aagaagaaat cattgaggat
    19741 aagcggctgg aaaatttcag aaactacagt caagcaaaag gaatctatca tgatgaactg
    19801 atctttcaag gaaactttac tgcccagtct ggctatgact tgatgaagga ggccattcag
    19861 agcttgggag accaacttcc gccagcattt ttcgcagcca gcgatagttt agctatcggt
    19921 gccctccgtg ccctccaaga agctggaatc aacctgccag atcgcgtcag cctcatttcc
    19981 tttaacgaca ctagtctgac caaacaggtc tatcctcccc tctctagtat tacagtttat
    20041 actgaagaaa tgggccgagc aggtatggat attcttaaca aggaagtcct ccacggtcgg
    20101 aaaatcccta gcctgaccat gctgggaacc agactgacat taagagaaag taccctaaat
    20161 caagaatagg ataacataaa aaacgaatag gagttttaga actcctattc gttttttatt
    20221 cgattataat catagactta atggtcttac gttcatccat atctttgtag gcttggtcga
    20281 tatcttccag tttataactt gaagtaaaga cgcgacctgg attgatatca ccatcaagga
    20341 cggcttttag taaaaattgc ttatcgtatg ttgtagcaga agctgcccca cctgctacag
    20401 agatattttg cataaatgtc gaaccaagag cacgattatt atagtgtggg actcctacaa
    20461 agcccatacg ccctccatta tgaagaacac ctagcgcctg ttctatagca gcctccgtac
    20521 caacacattc aagtgctgca tctgctcctc caccgaggat ttcacgcacc ttggtaattc
    20581 cttcttgacc acgttctgca acaacagctg tcgcacctga ctccatagcc atcttttgac
    20641 ggtcttcatg acggctcata aggataattt gtgatgctcc acgcatctta gccgcgatga
    20701 cagcacattg accaacagcc ccatcaccga taacaacaac cttgtcccct ttttgaacat
    20761 ttgcaacacg cgctgcatga tagcctgtcg gcatgacatc tgcaagagtc aaaagggact
    20821 tgagcatccc ttctgtataa tcagaaggtt gaccagggat tttaaccagc gcccagtttg
    20881 catagtggaa gcgaatatat tctgcctgaa aatcaccccc caaattattg ccaatatgat
    20941 tgtcgcaaga accgtcaaat ccagcaagac aggcatcaca ctcaccacat ccatgtgtaa
    21001 aagggacaat cacaaaatca cctggtttca ccgtcgtaat ggcttcccca gcttcttcaa
    21061 caatcccaat cgcttcgtgt ccactatttt tatgtccagc tttcgtttct ggattacggt
    21121 acctccataa atctgaacca caaacgcacg cacgaaccac acgaataatc acatcatccg
    21181 cttctattat ttgcggacgt tcaatgctag caagtccaac ctgacctgcc tttgtatata
    21241 ctgctgattt catttaaaat tttccttcct tataaagttt aattttgaga tttaaacgat
    21301 ttaaagctgt ctgtagctga gacaagcgct cctctaattt ttgcttttcc tcttctaaaa
    21361 taccaagcct ctcctctctc gtttcatctc ccttttggta gagcgacata tagtcaacta
    21421 aactatctac agagacaccc gccgaacgaa aacacttaat aaattccagc gcttcgatat
    21481 cctgatcttg aaaatcacga atcccagtag cagtacgagt aatcggtggc acaagaccaa
    21541 cccgttcata ataccgaatc gtatccgctg aaattcccaa caaatcactg gcagatttaa
    21601 tattcacacg ctaacctcct ctcatttcat tcactgaatt cacgctattc taactcactt
    21661 atcttattat ataactttga gttgactcca agtcaagcat tttgtatcta atcgaaaaat
    21721 ttttatcatt aacaatcata tatccggagt ttatacaatg ctcttctctt accaattttt
    21781 gaaatattta aataataaag aaattactct attccaaata agatagtacc caagtatttt
    21841 tcactttttt ctaatgttga tgctcataac tccgttccaa gtcacacacc tttcgcttat
    21901 gggtttggtg agtttctagg tcagcatcaa tttcaatggt aatattttga aaaccacaat
    21961 cttttaggaa aattcgaata gactctttac aagtttccat atgttccatt tcttttagac
    22021 aaacatggac aatggcattt ttttccaaag catccatagt ccagagatta agctgattaa
    22081 ggctggccac attgtccaat cgctccaggc cactctttac ttgcttgata tcaagacctt
    22141 ctggcacagc atccaagaaa atcttgagtg tagaccaaaa acgtggaagg gcttttgaaa
    22201 gaataaagaa agaaatgaca agggacaaaa gaggatctag gatataccag tccgtaaatc
    22261 gaagaacaat cgccatcagg ataacagcta cccaccctag cgtatcttcc agaaaatgca
    22321 aactcagaat agactcattc tttgtctttc ccttaccaac caccagactc gctaacagat
    22381 tgatagtaat cgcaataatt cctaaccaga gaatcccctc atcattgact ggttgcggat
    22441 gcaaaatctt cgtgacattt tccaaaatga ctagaacaga gcccgttacg agaatcacag
    22501 ctgttaccaa ggctcctagc aggctaaacc gcttatagcc caaggtgtac tgattgtctt
    22561 cttcacgatt ggagattgtt tctagaaaag ctgatattcc aattgcaatc gcatctccca
    22621 agtcatgcac agagtcagca agaacagcgc tagaaccaaa tactccacct gcaataaact
    22681 caacaatggc ataagtcaaa tttaagaaaa aagccaccca aacagcatat tttgccttca
    22741 tatttctcat tcctttgtta taatagattt atgaacacct tgttcattat cattatccac
    22801 taaaactcaa agaaaggata gaagcaaaac gtcagcttta ttcagttctg aacaatttgc
    22861 cttaagtgtc catatgacta acattgaccg ccgtatcagc aaaaccaaaa aagccatcta
    22921 tcaagctttt atacaacttt tgaatgctaa gggctacgag gccactactg ttcaggatat
    22981 cattgatctc gcagatgtgg gaagatccac cttttactgt cactatgaaa gtaaggagct
    23041 tcttctggac cagctctgcc gctacctctt ccatcatctt tttgaaagag agcaagccat
    23101 ttcaaccgag gactacctcg cccacctctt tctccatttt cataaaaacc aagaccatat
    23161 caccagtctg ctattttcca aaaatgacta cttcctccgt caactccata aagagctaga
    23221 acaccatgtc tattccgtgc tagctgataa tttaaaagaa gcccacccta atatgcctac
    23281 ttcttacctc caacacttgg tcgtgtccaa ctttatcgag acattgacct ggtggctcaa
    23341 aaaaggtcaa gatttcacag gccaggaagt tgtccaattt tatctagacc ttctcattcc
    23401 taaaaattga atatagagta aagctcagtt gtcttatttc taggttactg agttttttat
    23461 cttttcaaca acaaaagagg acccgccgat cctctttttc atactataaa tccttgatta
    23521 tcaactatat ctgttttaat cgaaatctca aaacagcact ttcaaacatc ttttcctagt
    23581 taagtaaatc agtattctgc ttagctgcct tgctccattg ataccaacca actagactgt
    23641 taatgagata aattagatat ttcccttgaa tttgcaggct ttctccccac cagagataga
    23701 ttgaaaaaac attggtagcc gcccagaata tccactgttc acggtaaaca gctgtcatga
    23761 ggatttgccc taccccattg gttgcatctg tgattgaatc acgataggga cgattggcac
    23821 caatagactg ataaatgaag ccaaaggcca accaccaaag cacactaatg gaaagatact
    23881 ttgtccagcc cttgccgtcc agtttacgcg cgacaaactc ctgcttttcc ttcttaaact
    23941 gtgcctgata aatccaaact agaagtccaa ttggctgcat gactgtgaag taaagtgtcg
    24001 tcagcacctc accataaaag cctttctgta gggccaaaat aaggtaaata acagagttaa
    24061 tcaagccaaa aagataatta cttgctcgac cttccgatac aaagattaca cagataatcc
    24121 ctgtcaagct acaaatcatc ccaatccagt caacaatacg atgttcgtaa accaactcca
    24181 gccagagagg aaaacttcct aaaaccagca aataaatcca ctgggcaaaa ctacgatggg
    24241 caaagaggtc atcccagata gccttcatag ttcctgaaaa tcctaaatca gccatagccg
    24301 caaccatacg acggtaacca cctgacattt cacctagggt tgttttgata ttttcaattt
    24361 tcttttgcaa ataagtatgc atcatttctc cttttgtttt taaagagccg tgtctggata
    24421 gactttcgga cgcaacgctc tattagataa tgaactgcct atacacaaga tttctaacct
    24481 tagtcgacat gagctgaaac ctcttatttg ttaagtagtt cacaaaatat tatacaccta
    24541 ttttatgaat agtcaactgt ctttacagta aaattttaga aaatcatgaa aattttctct
    24601 ttctttccat tttaagtgac attcagtcat tctcacatca aaaaagccca gacgaaattg
    24661 tctgagcatt cttttatcta gtcgtttaag gaagttgagt tcagtatgtt taaagtctct
    24721 gtcccatcat ttcttcaaca aaccttgttc ttggagaaac tccttggcta cttgctttgc
    24781 tgacttgcct tcaacaccga cttggtagtt gagctggctc atctggcttt ctgtaatctt
    24841 accagccaat gtattaagaa ctctttccaa ctctgggtgt ttcttgagaa gagcttcttt
    24901 catgagtgga gccccttgat aaggtgggaa gagttgcttg tcatcttcca agacctgtaa
    24961 atcataacgc tccaattccg catcagtcga ataggcatcc gtgatttgaa tatcccctga
    25021 ctgaattgcc tgataacgaa gagctggctg catagtcgcc acattgagat tgagaccata
    25081 cattgattgc aagcccttat ttccatcttc acggtcgtta aactcaagcg taaagcctgc
    25141 cttcagctgc ccttccactt ttttcaaatc cgaaatggtc ttcaagccat attcttgagc
    25201 aatctttttc ggaacagcta cagcataggt attttgataa gacataggtt tgagataggc
    25261 tagatgatcc tgcttggcaa tgccatcacg cgccacttga taaacctgct ctggctcatg
    25321 actcaccttg ggtgatggtt gaagcaaact ttcagtcacc gtaccggtaa attcaggata
    25381 aatgtcaata tcaccttttt tcagagcttc ataaaggaaa cttgtcgtcc caaaattcgg
    25441 tttaacagtc gcagtcatgc tggtattttc ttcaatcagc aatttataca tattagccaa
    25501 aatttctggt tctgggccta ttttcccagc aataatcaag ttttccttct ctttttgagc
    25561 caaaagggct ggactataag acagacccag taataaagcc accaaggcaa aaccagagaa
    25621 aatcgtccgc aatttcgctt tttccattac ttttagtagg aagttaaagg caatagctag
    25681 cactgcagaa gaaagggccc caatcaaaat caaactggca ttattacggt caattcccaa
    25741 caggataaag gaacccagtc cccctgcccc aatcaaggtc gccaaggtag ccgtaccgat
    25801 aatcaagacc gctgccgtcc gaattccaga catgataaca ggcatggcaa gtggaatttc
    25861 aaacttcttg agacgctccc atctggtcat cccaaaggca atcccagcct cttgaagact
    25921 cggatcaatt cccttcagcc cagtaatagt attttgtaaa atcggaaaaa tcgcataaat
    25981 cactagagct gtcaatgccg gtaaagtccc aattcccatc aagggaataa agagtcccaa
    26041 caaggccaga gacgggatgg tctggaaaat ccctgcaatc tgtaagaccc aatccgccaa
    26101 cttctcatga tagcgaagat aaacagccaa gggaatcgca agcaaaatag ctagtaacaa
    26161 ggtcaaaagt gacaactgca aatgttgaga tagggctgtc aaccaatcac taaaacgatc
    26221 ctgaaaagtt gcaattaaat tagtcatgaa cactacctcc aaacaagtct gctacaaagt
    26281 ctgttgcagg cacttttaaa attgtctcgg gattcgctac ctggcgaatt tctccatcct
    26341 gcaagacagc aatacggtcc gccaacttca aggcttcatc cgtatcatgg gttacaaaaa
    26401 tcgttgtcat cccaaactct ttatgcaatt cttttgtcag aacctgcaac tgttttctcg
    26461 aaatagcatc caaggccgaa aagggttcat ccatgaggaa aatcttgggc tgaccaatca
    26521 tagctcggac aataccgacc cgttgctgtt ctccaccaga tagctcactt ggaagtcgat
    26581 gcccatactc agctactggt aatccaactt ttgccaaaag ctcttctgtt ttttgagcaa
    26641 tttcttcctt agtccaaccc ttcatttcag gaatgagagc aatattttcc tcaactgtta
    26701 gattgggaaa gagagcaata gcctgtaaaa catatccagt agtaagacga agttcacgct
    26761 catcatagtc tttgatgcgc ttgccatcca tataaatatt tccatcagtt ggttccaaga
    26821 gacggttaat catcttaatc atggtcgtct tacctgaccc agaaggccca actaaaacca
    26881 tgaactcccc attctcaatc tgtaaattga catctctcaa gacatctttt tctgtgtagc
    26941 gcagtgctac atttttgtat tcaatcattc tttgtcctca atttaaaact tccctcgatt
    27001 agtcaagtct tctaccttag gtatgacttc cttattatcc caatgctcca caattttccc
    27061 gttctctaaa cggaagatat cgtactgggc ataagcaacg ccatcaatct gagtctgacc
    27121 atagctaacc acatagtttc cttgtcctaa gagttggaaa acaaagtcaa aagtgacact
    27181 atattcagcc acatagtttt tataagcagc acttccttgt ccaatctcat gattatgctg
    27241 aatcaaatcg tctgccacat aatcactcca ctgctctagc tccccatttt ggaaagtttc
    27301 tgtcaagaaa cggcgaacca gctttttatt ttctgctttc ttgtccaaat ccttgatttc
    27361 aaaatctcca aaaatttgat ctagttggtc attttcaggt gttcgatagt agtcaatgac
    27421 atcccaatgc tcaacaatac gaccattctc atccgcacgg aaagtatccg tcgtcaccca
    27481 ttgagcttct ccaccattca gatattgatg aacatgaaca aagaccagat tgccatcctc
    27541 aatggtgcgg acaatcttaa tctgacgctc tggatgacgc tcaaagaaat ctgcaaagaa
    27601 ggctgcaaat ccttctttcc cgtcaggaac acctgtcgaa tgttggatat aggtatcccc
    27661 tacagactgg gcttgagcct cagcaactcg tccgtcttga atggcatgga tgtataggtt
    27721 gtgagcattt ttcacttgtt gtgacatatt ctaaacctca ttttccttct ctttcagatt
    27781 tgccaaaatt ctttcttgaa aaccttcaaa ttggtgaatt tcttcctctg aaaatccttt
    27841 gtaaaagata gtatccaatt tctgactgac acgatgcccc acttctttct ggaacttgcc
    27901 taactccgtt aaaactaaat acttcttacg cttgtctttt ccacacggac taacaattac
    27961 aagcttttgt tcctctagct tttttatcat agtcgtcagc gtattattcg caagcccagt
    28021 cgcaagcgcg atatctgtcg cagttgcgca gccagtttca ctattccata aaaccgctaa
    28081 aatcttgccc tgttcacccc tataaagagc ctcaggatct tgactcagta acttttgaaa
    28141 aatccgccca ttcaacaaac gaatatgatg ggctagcaaa tgactatctt tcataacacc
    28201 tccaatttat ttcgatatcg aaatgaataa aataattgta acactcatcg ttctaactgt
    28261 caactatttc gatttggaaa taatttttga taattattca caccaccata ctccggctca
    28321 actaactttt aacgagagtt tctaaattcc ttcgtcctcc agtctacaaa agccttccat
    28381 ccgtactatc ctatttttta tgaggggaca catttttcct atcagatcat ttattttaaa
    28441 gatagaagta aatcataatt gcttccatct gttcttttat agtatattga agttggacta
    28501 gggcactgta tcttctaaaa cattgataga aagcgatttg aatttcccaa tcaatttgtt
    28561 cgtatttata gcattttgaa actagaatag gacgccatga ctgctaaaag atttctataa
    28621 attcatttga tttccctaat caatttgttc atatcttatt tcattccgct ataatttcac
    28681 cttaccctat ctttttcgta gcacccttca aacagcctat cccctaccgt ttgatgattc
    28741 ctcacttcac tccacttcca ttacagaagt ttcttcacta ctatggtctc ggctgacttc
    28801 tcatgattcc ttgttactac tatttgaacg ctcacgagat aggtcttata aaaaatgctt
    28861 tgatccacaa tggaatcaaa gcattttaaa aagttcctca tacataagcg cagaagtcgc
    28921 agttcctctg tacttggctt cttctctttt gacaaagcga gccaagttga gcaactcagg
    28981 tgctggatgt ttgggattta ggagcaattc acgattgacc aggcttgaga gacgaactgc
    29041 ctgcaattgc tcatttgtag taggcagttt tttagtagtc tctaggagag cagcaactaa
    29101 atcttcactc aaatcatgtc gagcatgatt gtaaagatct tttataaggc tttctaggtt
    29161 tggttctacc atccctacca cctcccttat ggtttaataa tgtttaatca aatcaaccgt
    29221 tgaacgatcc aatttcttca ccaaggcttg taagaaagct tgcgcttcta ggaagtcatc
    29281 cattgcatag agggtttggt gagaatggat ataacgagcg cagacaccga tagttgttga
    29341 tgggacacca ccatttttca gatgagctgc acctgcatct gttccgcctt taccacagta
    29401 gtattggtac ttgataccag cttcttcagc cgttgtcaaa aggaaatcct tcatccctgg
    29461 gagaagcaag tgacctggat catagaaacg aatcaaggtt ccatctccaa tcttgccttg
    29521 accaccgtag acatcacctg ctggtgagca atcaactgcg aggaagactt ctgggtcaaa
    29581 cttggttgta gaggtatgag cgccacgcag accaacttct tcttggacgt tagaacccag
    29641 atagagttca ttgccgagtt tttgacctga taaagcttca gctagctcgc ttaccatgag
    29701 gacaccgtag cggttatccc aagcttttga gatgatattt ttttcattgg ctgtcaaaat
    29761 tgcagaacta tctggtacga tggtatcacc aggacggatg ccaaaacttt ctgcctcagc
    29821 cttgtccgca aaaccaccat caaaaacgat atcggcaatg gctggcatgg ttggtccccc
    29881 ctttccacga gtcaaatgcg gaggaacaga acctgaaatc acaggaattt catgaccatc
    29941 acgagtcaag agtttgaaac gttggctgct aaccaccatg gggttccagc caccgatttc
    30001 tacgacacgg aaggtaccat ctggcttgat ttcgctgacc ataaaaccaa cttcgtccat
    30061 atgagaagcg accaagacgc gcggtgcatc cacagcttct gaatgtttga taccaaaaat
    30121 accacccaag ccatctgtca ccacttcatc cacatgcggt gtcaactttt cacgaagata
    30181 agcacggaca ggcgcttcat gacctgagac tgcagcaagt tctgttactt ctttaatttt
    30241 tgaaaataat gttgtcattt cagttccttc tttctttcat ccattttacc actttttata
    30301 ggagaaggat agtgggaagg tggatttcta agttagtatc ttagtcctgc tctatcttag
    30361 aaaaggatag tattctcttg catgtagtgc aaaatctagt aaacattcca aaattaactc
    30421 gaatatttat ttccaaacaa aaaaacaata caccatcaaa gttgtttgga tttttcatga
    30481 aatttacaga aaatagttga cttccctttc ttctttcttt aaatatatag ttggttgagt
    30541 ttggaatagt acgctgtagc tgctgaaaca tttctagaaa ttaatttgac tttcctaata
    30601 gagttgttca tatcttattt caatttacta tagtacaaaa ctagaaaagg aaaaaatcat
    30661 gaccaaaaca attcttgtta caggtgggac tagctacatt ggctcccata ccgttaaagc
    30721 tcttttaaat gctggctata aggtgcatgt cctcgataat ctctccacag gaaatcgagc
    30781 ggctgtggat agtcgtgcta gctttaaaga actggatgtt tatgatgcta gcgccttaaa
    30841 ggcttactta gaagaaaatc agattgatgc tgtcctccat tgtgcaggtg aaattgttgt
    30901 gagcgaaagt attgaaaatc caagtaaata cttcactgcc aacgttgctg gtatgaacca
    30961 agttctcaaa gtcttatctg aagttggcat tcaaaaaatc atgttctctt cgactgcttc
    31021 tctctatgga aataactgta ttgacaagcc ggtgactgaa gataccctgc tcgaccctgt
    31081 caacccttat gcagagacaa aactgatggg cgaacgaatg atttactgga tggccaatcg
    31141 ctacgactgg aaatatgtta ttttccgtta ctttaatgtt gctggggctg aaatggatgc
    31201 ttcaaacggt ctgcgtgtga aaaatccaac tcacatcatt ccaaatatca acaagaccgc
    31261 attgggacaa aatgatagct taaaaatatt tggagatgac tacgatacac gtgacggttc
    31321 atgtattcga gattacattt atgtcttgga ccttgcatag gctcatgtta aaggaatgaa
    31381 ctacctattt caagaagaca gttcttctca aatctttaac ttaggaacta aaaaaggcta
    31441 tactatcaaa gaaatcttta aaactgctga agaattacta aattaaaaaa tcccacacga
    31501 gattgttgct cgacgtgcta gtgacccagc cagtgtccta gcagatgctt caaaagctaa
    31561 acaatatctt gattggacgg ctagctactc cctcgaagat attattttat cagattaagg
    31621 taaaaacatt ttagaatagg ataacaaagg agaaagtctt gttgctctct ccttgttgta
    31681 tttattaaat tgtcagaaat tttattgaca gattagaaaa ataatgttac atttatatcc
    31741 gcagatatct ttcgatacca aatctacatg aaaggacggg gtatgaaact ttctcattat
    31801 ttaattggct tacttctact cctagtcttt ctctctatta gcattgggac cagtgatttt
    31861 tcatggggaa agctatttga tttcgaccag cagacctggc tcctctttca agagtcccgt
    31921 ctcccaagaa ctatcagtat tctcctgact gcctctagta tgagtatggc aggccttctc
    31981 atgcagacta ttacccaaaa tcagtttgct gcaccgagta cagttggaac gactgaagcc
    32041 gccaaactgg gaatggtgct gagccttttt gtctttccat cggctagtct gacccaaaag
    32101 atgctcttcg cttttgtttc atccatcgta ttcaccctct tcttcctagc ctttatgacc
    32161 atttttactg taaaggaaag gtggatgttg cctctgattg ggatcatcta tagcgggatt
    32221 atcggctcag tcacagaagt tatcgcctat cgtttcaatc tggttcagag tatgactgcc
    32281 tggacccagg gctccttctc catgattcag acccatcagt atgagtggct cttcttaggc
    32341 ctcatcatcc tgataaccgt ttggaaatta tcccaaacct tcaccatcat gaatctaggg
    32401 aaagaaacca gcgagagttt ggggatttcc tactccctac ttgaaaaact ggccctcttt
    32461 ctggtggcgc taacgacaag cgtcaccatg attaccgtgg gtggcctacc atttctcgga
    32521 gttatcgttc ccaatcttgt tcgcaagcgc tatggagata atctaagtca aaccaaactc
    32581 atggtcgcac tggttggtgc caatctagtt ctggcttgcg atatcctatc ccgagttctg
    32641 attaggccct atgagttgtc tgtcagtctc ttgctaggaa tcatcggtag tctcgtcttt
    32701 atcctacttc tctggagagg gggacgaaaa gatgcagact aaaagcaaac ataccaagct
    32761 cttctggatt ctcattattc ttgccatcgg agcttgtctt ctctactttt ggcccatcac
    32821 tcacttgtca gcctttgctt ggaagttgcg ttcccaaaag atcatcgttt atctcttggt
    32881 agccatcgcg actgggattt cgaccattag ttttcaaacc ctgacggaaa atcgcttcct
    32941 gacgcctagt attttgggaa ttgaatcctt ctacgtccta ctacaaaccc tactactggt
    33001 ttttgaaagc aagtttcttc aacttggcaa atcccctatc ttagaattcc tagtcttact
    33061 tcttgtccag tccctcttct ttctcgcctt acaaggttac ttgaagacac tgatgaagca
    33121 agacctggtc ttcatcctgc tgatctgtct agcgctcaga agtctctttc gaaatatcag
    33181 caccttcctt caagtcctaa tggatccaaa cgaatacgat aaactgcaaa atagtctttt
    33241 tgcctccttt caacatctca acacttccat cctagccatc ggttctctga tcatcctcgc
    33301 tttgacaatc tttttctttc gaaaagcagt cgttctagat gtcttgcacc tgcaaagaga
    33361 aacggctcag atattgggac tcgatgttga aaaagaacag aaagagctcc tctggggaat
    33421 cgtgcttttg acctcaacgg ccactgcctt ggtaggacct atggccttct tcggctttat
    33481 gctggccaac ctcacctacc tgattgtcaa agactatcag cacaagttac tctttatagt
    33541 ggccattctg gttggattta ttagcttaac cttggggcaa gccttgattg aacgagtctt
    33601 tgcactggaa attcgtatca gtatgatcat tgagagtgtg ggtggcttct tattctttat
    33661 cttactatat aggagggctc gtcagtgaaa ctggaaaaca ttgacaaatc cattcaaaaa
    33721 caggatattt tgcaaggcat ttcgcttgaa gtcagtcctc aaaaactgac agcctttatt
    33781 ggtccaaatg gtgctggaaa atcgactctc ctctccatca tgagcagact aaccaagaaa
    33841 gatcagggag ttctcagtat caaaggacgt gaaatcgaga gctggaattc gcaagaactg
    33901 gcccaagaac taaccatcct aaaacagaaa atcaattacc aagccaaatt gactgttgaa
    33961 gaactggtca gttttggacg ttttccctac agccgaggtc gacttagatc agaagactgg
    34021 gaaaaaatcc gagaaactct gaactatttg gaactgacca acttaaaaga ccgctacatc
    34081 aatagcctgt caggggggca actccagcgc gtctttatcg ctatggtact ggcccaggat
    34141 acggacttta tcttgctgga cgaaccactc aacaatctcg atatcaagca aagcgtcagc
    34201 atgatgcaga ttcttcgacg actggtggag gaactcggca agaccattat catcgtcctc
    34261 cacgatatca acatagccag tcagtatgca gatgaaattg tcgccttcaa ggacggccag
    34321 gtctttagca agggaagaac cgatcaaatc atgcaggctg acctgctcag tcaactttat
    34381 gagattccca tcacgctagc tgatatcaat gacaaaaaga tctgtatcta tagctagtaa
    34441 cataaaagct caagttagag aaccttcagt ctcttagtca ataagatcaa gagactccct
    34501 aaatcgttat cacattttaa agaggagaaa ttatgaaaac atcccttaaa ctttatttca
    34561 ctgccctagt ggccagcttc ttgctcctac ttggtgcatg tagtacaaac tcaagcacta
    34621 gtcagacaga gaccagtagc tctgctccaa cagaggtaac cattaaaagt tcactggccg
    34681 aggtcaaact ttccaaagtt cctgaaaaga ttgtgacctt tgacctcggc gctgcggata
    34741 ctattcgcgc tttaggattt gaaaaaaata tcgtcggaat gcctacaaaa actgttccga
    34801 cttatctaaa agacctagtg ggaactgtca aaaatgttgg ttctatgaaa gaacctgatt
    34861 tagaagctat cgccgccctt gagcctgatt tgattatcgc ttcaccacgt acacaaaaat
    34921 tcgtagacaa attcaaagaa atcgccccaa ccgttctctt ccaagcaagc aaggacgact
    34981 actggacttc taccaaggct aatatcgaat ccttagcaag tgccttcggc gaaactggta
    35041 cacagaaagc caaggaagaa ttggccaagc tagacaagag catccaagaa gtcgctacta
    35101 aaaatgaaag ctctgacaaa aaagcccttg cgatcctcct taatgaagga aaaatggcag
    35161 cctttggtgc caaatctcgt ttctctttct tgtaccaaac cttgaaattc aaaccaactg
    35221 atacaaaatt tgaagactca cgccacggac aagaagtcag ctttgaaagt gtcaaagaaa
    35281 tcaaccctga catcctcttt gtcatcaacc gtacccttgc catcggtggg gacaactcta
    35341 gcaacgacgg tgtcctagaa aatgccctta tcgctgaaac acctgctgct aaaaatggta
    35401 agattatcca actaacacca gacctctggt atctaagcgg aggcggactt gaatcaacaa
    35461 aactcatgat tgaagacata caaaaagctt tgaaataagc cacttcaaac tcaatttaat
    35521 actcaa
//
LOCUS       DBEJAQ010000019        35148 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.019, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000019 DBEJAQ010000000
VERSION     DBEJAQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.019"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(99..449)
                     /gene="rbfA"
                     /locus_tag="ACL297_000983"
     CDS             complement(99..449)
                     /gene="rbfA"
                     /locus_tag="ACL297_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGR0071263.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(700..3492)
                     /gene="infB"
                     /locus_tag="ACL297_000984"
     CDS             complement(700..3492)
                     /gene="infB"
                     /locus_tag="ACL297_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGR0071264.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(3509..3808)
                     /locus_tag="ACL297_000985"
     CDS             complement(3509..3808)
                     /locus_tag="ACL297_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HGR0071265.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(3801..4094)
                     /locus_tag="ACL297_000986"
     CDS             complement(3801..4094)
                     /locus_tag="ACL297_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGR0071266.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(4116..5252)
                     /gene="nusA"
                     /locus_tag="ACL297_000987"
     CDS             complement(4116..5252)
                     /gene="nusA"
                     /locus_tag="ACL297_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGR0071267.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(5296..5775)
                     /gene="rimP"
                     /locus_tag="ACL297_000988"
     CDS             complement(5296..5775)
                     /gene="rimP"
                     /locus_tag="ACL297_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGR0071268.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(5901..6536)
                     /gene="trmB"
                     /locus_tag="ACL297_000989"
     CDS             complement(5901..6536)
                     /gene="trmB"
                     /locus_tag="ACL297_000989"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGR0071269.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(6533..7327)
                     /gene="ccrZ"
                     /locus_tag="ACL297_000990"
     CDS             complement(6533..7327)
                     /gene="ccrZ"
                     /locus_tag="ACL297_000990"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HGR0071270.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(7488..8099)
                     /locus_tag="ACL297_000991"
     CDS             complement(7488..8099)
                     /locus_tag="ACL297_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGR0071271.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            complement(8250..8483)
                     /gene="blpZ"
                     /locus_tag="ACL297_000992"
     CDS             complement(8250..8483)
                     /gene="blpZ"
                     /locus_tag="ACL297_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein BlpZ"
                     /protein_id="HGR0071272.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            complement(8525..9214)
                     /locus_tag="ACL297_000993"
     CDS             complement(8525..9214)
                     /locus_tag="ACL297_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGR0071273.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            complement(9266..9649)
                     /locus_tag="ACL297_000994"
     CDS             complement(9266..9649)
                     /locus_tag="ACL297_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HGR0071274.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(10278..10597)
                     /locus_tag="ACL297_000995"
                     /pseudo
     CDS             complement(10278..10597)
                     /locus_tag="ACL297_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
     gene            complement(11117..11236)
                     /locus_tag="ACL297_000996"
     CDS             complement(11117..11236)
                     /locus_tag="ACL297_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGR0071275.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(11340..11489)
                     /gene="blpO"
                     /locus_tag="ACL297_000997"
     CDS             complement(11340..11489)
                     /gene="blpO"
                     /locus_tag="ACL297_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HGR0071276.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(11733..11981)
                     /gene="blpK"
                     /locus_tag="ACL297_000998"
     CDS             complement(11733..11981)
                     /gene="blpK"
                     /locus_tag="ACL297_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HGR0071277.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(12050..12319)
                     /gene="blpJ"
                     /locus_tag="ACL297_000999"
     CDS             complement(12050..12319)
                     /gene="blpJ"
                     /locus_tag="ACL297_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HGR0071278.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(12786..12983)
                     /gene="blpI"
                     /locus_tag="ACL297_001000"
     CDS             complement(12786..12983)
                     /gene="blpI"
                     /locus_tag="ACL297_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HGR0071279.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            13265..13852
                     /locus_tag="ACL297_001001"
     CDS             13265..13852
                     /locus_tag="ACL297_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGR0071280.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            13779..15422
                     /locus_tag="ACL297_001002"
     CDS             13779..15422
                     /locus_tag="ACL297_001002"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HGR0071281.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            15433..16794
                     /locus_tag="ACL297_001003"
     CDS             15433..16794
                     /locus_tag="ACL297_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HGR0071282.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            16851..16979
                     /gene="blpC"
                     /locus_tag="ACL297_001004"
     CDS             16851..16979
                     /gene="blpC"
                     /locus_tag="ACL297_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HGR0071283.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(17050..18390)
                     /locus_tag="ACL297_001005"
     CDS             complement(17050..18390)
                     /locus_tag="ACL297_001005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGR0071284.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(18404..19141)
                     /locus_tag="ACL297_001006"
     CDS             complement(18404..19141)
                     /locus_tag="ACL297_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGR0071285.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(19146..19484)
                     /locus_tag="ACL297_001007"
     CDS             complement(19146..19484)
                     /locus_tag="ACL297_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGR0071286.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            19760..20089
                     /locus_tag="ACL297_001008"
     CDS             19760..20089
                     /locus_tag="ACL297_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGR0071287.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(20257..21306)
                     /locus_tag="ACL297_001009"
     CDS             complement(20257..21306)
                     /locus_tag="ACL297_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071288.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(21303..22034)
                     /locus_tag="ACL297_001010"
     CDS             complement(21303..22034)
                     /locus_tag="ACL297_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071289.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            22102..22512
                     /locus_tag="ACL297_001011"
     CDS             22102..22512
                     /locus_tag="ACL297_001011"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGR0071290.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            22522..22809
                     /locus_tag="ACL297_001012"
     CDS             22522..22809
                     /locus_tag="ACL297_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071291.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            22971..23255
                     /locus_tag="ACL297_001013"
     CDS             22971..23255
                     /locus_tag="ACL297_001013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071292.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDTTDEVSSKHCFEVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(23567..24703)
                     /gene="dnaJ"
                     /locus_tag="ACL297_001014"
     CDS             complement(23567..24703)
                     /gene="dnaJ"
                     /locus_tag="ACL297_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGR0071293.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(25105..25461)
                     /locus_tag="ACL297_001015"
     CDS             complement(25105..25461)
                     /locus_tag="ACL297_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HGR0071294.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVPAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(25463..27286)
                     /gene="dnaK"
                     /locus_tag="ACL297_001016"
     CDS             complement(25463..27286)
                     /gene="dnaK"
                     /locus_tag="ACL297_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGR0071295.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(27766..28290)
                     /gene="grpE"
                     /locus_tag="ACL297_001017"
     CDS             complement(27766..28290)
                     /gene="grpE"
                     /locus_tag="ACL297_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGR0071296.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(28317..29351)
                     /gene="hrcA"
                     /locus_tag="ACL297_001018"
     CDS             complement(28317..29351)
                     /gene="hrcA"
                     /locus_tag="ACL297_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGR0071297.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(29515..30024)
                     /locus_tag="ACL297_001019"
     CDS             complement(29515..30024)
                     /locus_tag="ACL297_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HGR0071298.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCIATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            30632..32965
                     /gene="hsdR"
                     /locus_tag="ACL297_001020"
     CDS             30632..32965
                     /gene="hsdR"
                     /locus_tag="ACL297_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGR0071299.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAMVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            32978..34441
                     /locus_tag="ACL297_001021"
     CDS             32978..34441
                     /locus_tag="ACL297_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGR0071300.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            34441..>35148
                     /locus_tag="ACL297_001022"
     CDS             34441..>35148
                     /locus_tag="ACL297_001022"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGR0071301.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDY
                     NFNLLLIALPPLSEQQRI"
BASE COUNT        10962 a         7364 c         6278 g        10544 t
ORIGIN      
        1 gcgtactatt ctagtttcaa tatattttaa aagaatattt ttccatttca aattcaacct
       61 attttcctcc accaaaaaag aggggcaacc cctcttcttt agttcttatc cagattgcgt
      121 agcatctcgt caatcttgtt tccatactcg atggactcgt ctttgacgaa ggtcaaatct
      181 gggattttgt acaatttcaa attgcgacca agttcacgtt tgatggtacc agttgctttt
      241 tcaagcccga tttgggcttt ttggttatcc gaagcaaggt tactcaaaat ggtgtaataa
      301 accttggcaa cagacaagtc acccagcatc tgaacatctg tgatggtcac accttggaca
      361 cgtggatcac ggactttctt ttgcaaaatc tcattgactt cacgcttgat ttccatgccc
      421 acacgatctg tacggaaatg atttgccatg atattccttt ctctactttg aaccaaaagc
      481 taggccagca gacctagcta ttttaaactt cttgattttc agtttaaatt gaaacgaagt
      541 tcaatttgaa gtcatctgct tctttcgccg tggtgaaagt gatggaactg atttatcagt
      601 cccatcacag gggatttttg agaccctagg ctcaaaaata agcaatgaaa ccatcggttt
      661 gcttgcgtcc ctcaccacct aagaaaggag caaaaaatct tatctcttga tttcttccat
      721 gacatacgcc tcaatcacat catccatctt aatatcattg tagccgtcga tcatcaatcc
      781 accttcacga ccgtttgtca cttctttcac gtcgtctttg tagtgtttca agcttgcgag
      841 ttcgccatca tagataacga caccatcacg gataacacgg actttagagt cacgggcaac
      901 cttaccgttg ataaccataa atccaccgat agttcccact ttagacacct tgaaggtttc
      961 acggataacc gcttcaccaa taactttttc ttcaaattct ggatcaagca tccctttcat
     1021 agcttcttcc atctcttcga taaccttgta gataatgctg tgaagacgga tttccacatc
     1081 gtcagcttct gcttgttgac gagcttgtgg tgtagggcgt acgttgaaac caacgataaa
     1141 ggcatttgaa gcttcggcaa gggtcacgtc tgattcgttg atagcaccga ccgctgagtg
     1201 gacgatagtc actttgacac cttccacgtc aatcttttga agtgaggcag aaagggcttc
     1261 aacagaacct tgtacatcag ccttgatgat aacattaaca gatttgagtt ccccagcttt
     1321 aagggtatca aagaggtttt caaggctaac acgttgggta gcttgacgtt gtttcatgag
     1381 ggcacgtttg gcacgctctt cacctgctgc acgcgcagat ttttcatcct cgtaaacggc
     1441 aaagtggtca cccgccatcg gtgcttcgtt caaacctgtg atagagactg gtgttgatgg
     1501 tccagcaact ttaacacgac gaccaaggtc gttggtcata gcacggacac gaccgaaggt
     1561 atttccgaca acgattggat cttgaacatt caaggtacct tgttgtacaa gaagggttgc
     1621 gaccgcacct tttcctttat ccaagcgcgc ttcgataacc gtaccgattg cacgcactgt
     1681 tgggtctgcc ttgagttctt ggatttcagc tacaagaagg actgtttcca acaattcttc
     1741 gatattttgg ttgaatttag ctgagatttc aacaaattca gaatctccac cccaagcggt
     1801 tgacatcaca ccatgctctg ccaattcacc gataacgcgt tctgggttag cacctggttt
     1861 atcaatcttg ttaatagcta cgatgattgg aacgttagct gcttttgagt ggttgatggc
     1921 ttcaatagtc tgaggcataa ccccgtcatc tgccgctacg accaagatcg taatatcggt
     1981 aacagaagca ccacgcgcac gcattgatgt aaaggccgcg tgtcctggtg tatcaaggaa
     2041 ggtaatcttc ttaccatttt ccacgatttg gtaggcaccg atatgctgag taataccacc
     2101 tgcttcacct gtcgcaacac gtgagttacg aagagtatcc aaaagggttg ttttaccgtg
     2161 gtcaacgtgt cccatgatag taacaactgg tggacgctca accaattcat cttcattgag
     2221 ataaccatct tcgacaaaga aacgttcgat gtcagcatta tccacttcaa ccttttgttt
     2281 ggcttcgata ccgtaatcca ccatgaggag ttcaattgtt tccccatcca aggattggtt
     2341 ttgtgtggcc atgacaccca tcatgaaaag tttcttaaca atttcagctg gttcacgttt
     2401 gatacgtttt gcgatttccg caacggtcat accatctgta tattcaaatt ctgttggcaa
     2461 ttcatggaat ttacgctccg taacaggttt tggagtctga ttacggttgt tcttgttatt
     2521 gccttttttg ttctttttgt tgttattcca gttactattc ttttgatttc tcacttgatt
     2581 ttgactactt cgattctttt gttgttttct aggaccatct tcttcatgat cataatcgtc
     2641 acgatttttg tctggtcgag cttgtttttt acgacgtgta tccactgcag gttctttttt
     2701 ctcagggacg acttcaacca ctgcttgaac ttgctgtgct tgttcagcta acttagccgc
     2761 ttcttcaaag atttcctctg gttccttgcg tttgttagct tgagccaagg cttctttagc
     2821 agcctgatac tgcttgaagc gttcctcact tgaacgagcg tactctgcat tttgctctgc
     2881 ttttagggct gctgcacggg ctttaaagtc aatacgtgga gccgcttgat ttgaacgttt
     2941 atcctcttgc tgacggcgct catttctacg tttttgctga ccttgctgct tcttagcttg
     3001 gtcattaaag cgacgattgt cgcggttgct ttgaccttgt tttccaccat tacggccgtc
     3061 gtttttctga cggtttccgt tttgttgttg gtcacggtta ttgcccttat tttgcttgcg
     3121 tcgttctgcc tgctctttag cacgggcttc acgctcagcc ttgaaattac gactttgcgg
     3181 tttcactgct actttttctg ttttcggagc aactggctca gccggttttg cctcttcctt
     3241 agctacagct ggtttagctg gctcagattt ttcggctttc ttttctgcac ttacttttgg
     3301 tgctgcaggt tttgcttcta ctttcggagc agctgcaggc ttaaagctgg cagcaatttt
     3361 tgcagcgaca gcttcttcca cacttgatga gtggcttttc acatccaagc ccaactcttt
     3421 tgcacgcgct acaacttctt tactttcttt tccaagttct tttgcgattt cgtacaatct
     3481 tttcttagac aaatcatgtc ctcctcttct attccataag agacctcatt ttctttgtaa
     3541 atccagcatc tgttacagcc aaaacctttc tcgatttccc gactgctatg cttaattcca
     3601 gtgttgaaaa cacggttaca atttctactt gataataatg acttttatct tgaatcttct
     3661 tggtcagatt gggtccagca tcatgagcta gaaagaccaa cttggccttg ccgtcttgaa
     3721 tggccttgac caccaattct tcacccgata tgatgcgccc tgctcgctga gcaagcccca
     3781 agagattact tatcttttgc ttattcaagt cccaactctc ttcttttcac tttgtgatcc
     3841 acataagcga tcaactcgtc ataaaagctt tcttccactt ccatgctaaa gctgcggtta
     3901 aagaccttct tctttttcgc ctctagggct tctgcattgt ctagtttgat ataagcgccg
     3961 cggccattgg ccttgcccgt aggatcaata aagacttgtc cttccttgtt cttgacaatg
     4021 cggagcaaat cacgcttatc aatcacttcg ttagacacaa cagacttgcg caaagggatt
     4081 tttcttgttt tcatctttcc ctcctctagc agcttttatt cttctacagt atcgttttct
     4141 acttccaact ctactgaagc agcgtcttcc atggcttcaa attcgctagc agacttgata
     4201 tcgatacggt aaccagtcaa gtgagccgcc aagcgcacgt tttgtccacg acgaccaatg
     4261 gcaagagaaa gcttgttatc tggaacaacc accaaggcac gtttgctgtc gttttcatca
     4321 aagataactt ggtcaacctc agcaggagcg atggcattgt agataaattc agctggatct
     4381 gctacccact cgataacatc gatattttct tcgattggta ccatgcggtc atttttagca
     4441 tcgtaacgag ctgggtggaa tttgctagta atcttcttga tattagcacc accacgtcca
     4501 acgattgtac cgatagcatc cacgtttgga ttgtggctac gaacagcaac cttcgtacgg
     4561 tcacctgctt cacgagccac gctcatgatt tcaacagttc catcataaac ttctggaatt
     4621 tcttgctcca ttaaacgttt gatcatttct ggatgactac ggctaacaaa gacgttcaca
     4681 ccacgagggt tgtcttcaac cttgtaaaca taaacttcga tacgatcatg agaagcaaaa
     4741 acttctccag gaatttggtc ttgttttgac aattgggctt cgatgctacc aaggttgaca
     4801 tagataaagc ggttgtcaaa gcgttctact gtaccagaca tgatttcttg ctcatgttct
     4861 ttgtaagtat tgtaagtgat ggcacgtgtt tgcttgcgca ttttttccat gatggtttgt
     4921 ttggcagact gggctgctac acgaccaaac tcagctggtg cttcttcaaa cttgattttg
     4981 tctccaagtt cataagctga attaatggca agagcatctt tcaagctgat ttccaaacgg
     5041 ctatcaaata cttcatcaac aacttcacgg acagtataaa ctgtaaagtc acctgttttt
     5101 tcgttgaagt caatagctac gctgtctgac tgaccatagc gtctgcgata agcggaacga
     5161 agcgactcta ctactgcgtc gatgatatct tcttttttga ttcccttgtc ttcttccaaa
     5221 atgcggaagg cctctagcat ttctttactc atgttttctt cacttttgaa tcctcaaaag
     5281 ctatcctttc tttttctata atttaactgc taaacgtgct tttgatacta aactgtatgg
     5341 aatttggacg gttttcttac gcgtcttgtc catatattcc atagtcaact cgtcctcttc
     5401 gaaggccaac aaggttcctt caaagacctt ttgcttatcg atggcttggt agagcccgac
     5461 atggatgtat tttccaaccg ctccagcgac ggcatccttg gttttcaaag ggcgttccaa
     5521 acctggactg gtaatttcta ggaaatattg ttctgggaag ggatctggct tgatggtgtc
     5581 taggacagga ctgataattt ctgtcaagtc tgccgtgtcg ttcaaggtaa ttccttcggg
     5641 tttatctaca aaaatactga gaatcatgtc actgccaatc tttccatact cgatatccac
     5701 gagttcaaaa ggagcttcta tgacaggttc tacaacttct ctgactaatt ctacgattgt
     5761 tgcgattgcg tccacctcct cataagcaag aggcgaagat atttccccgc ctcacttttc
     5821 atattcttac tatcagtata gcacgtttgc caacttatgt aaagcaaaag cacttgtaca
     5881 gcctaatttt aggctatctc ttaaaattct gcctcaactc ggtagataac ttgccccttg
     5941 tttgagaatt tttgctcgta ttctgtcatg acattgcctt caaaatcact ggcatgcaaa
     6001 tctaaccaga caccattgag tttcatgcca tattgagaaa agctcactaa actgtactca
     6061 aacaagccac ggttatccgt cttgaaatga atttctccat tttcaggcaa gatacgtttg
     6121 aaggtatcca agaaggtctt gtaggtcaaa cgacgctttt catggcgttt tttcggccat
     6181 ggatctgaaa agttcagata caagcgatca atctcaccgt cttcaaaata gtcagttaag
     6241 tcagaaccat ctacccacaa gagcttgatg ttaggcactc caacttcaag caccttgtcc
     6301 aaagcgtagc tcaaaacaga cttttgaata tcaatcccga tatagttgat gtcagggttt
     6361 tgcttggcca tacctgaaac aaaggcaccc tttccacttc caacttccac atgaatggga
     6421 ttatcattgc caaacaagtc ccgccatttt gccttggctt ccaagggatt gaggaccaca
     6481 tactggggat ttgcctctag taattctgtt gcccctttac gatttctaac tctcatcttc
     6541 tctttccata cttgtctcgg aaatgacgca aaccatgaat ctcccgattg acattttcta
     6601 aatcttggtt catataatac ttggaaatct gactcaaata agacaattga ccataccaat
     6661 acaatttact taataccgtt tgattgtact tgtaaccgta gtaggtcaac cattccttcc
     6721 actgatgttc tgaaatataa tggcagagca tatgggccac atcaaacatg cgatcggtca
     6781 agcgaaccga atcccaatct actaaataaa tcaagccact atctgtctca atccaattac
     6841 tatgtcgtac atctccatgg acaatggtcg catggtcctc tctaaatcct ggaatagtct
     6901 gacgtaaatc agccatcact tcactgataa aatgattttt acgcaaagca tctggagccg
     6961 tttcctgcca agactgtagt aaatctacag gtgtttccat ggcatagccc aaacgactca
     7021 attgtgtcat caacggacgt gagcgatgaa gacgggttaa aatattgacg atttgcttac
     7081 gattcatatc atagggggtc aatatcttgc ctgtcaacca ttcttgagca cacatatcac
     7141 gcccatctgc caaacgacga ctccataata attgtggagc aatttgttct ctagctagac
     7201 caggtaggat tggagaggtg ttcattttta caaagatgcg cttcccgtca ggatagctac
     7261 ccatataagc cttgccactt ttcccaggta tgggagtcag tgttagctca ttatcaccca
     7321 aatccatttc tttcctccgt ataaagtttc cttactattc tactagtttt tggcctattc
     7381 gtcaaccact ctaacccgat tctcaaagaa aagcctctgg attggcttgg gtatcattat
     7441 agcttgaaaa aggtaagcac catcttctgc ttaccttttc ataattttta taaataatat
     7501 aggagtggcc aactgttgta aaacatgtgg actagagtag aaacccatag gttttgactc
     7561 tttttataag caaagcccag caacaaaccc ccaagtagat aaaaaataaa cgaagggaca
     7621 ttagaaggtc catgcatgaa agaaaagaga gaggaagtta gcacaagccc taaccaagaa
     7681 ttgtcaaaaa ccgcaccttg aagaagtcct ctgaaaatga gttcctcctg gatggggcca
     7741 aaaactgagg tagtcaaaat caaagacagg gaaattcctc tgctgacttc cgccaagtgt
     7801 tggacccctg agccagaaga aacagcgaag aaatgaatat aaaccagcgt ctcaagtaca
     7861 cttagaagaa agattgcaat gaaaagcaaa agttgtttct tgcttaacat cccaaaagaa
     7921 atcatttcaa actttctagc ataagcaaca ctcagcgagg ttacaagaag acttacaaga
     7981 atcaatgcag attcattttg aaaaaaaccc ccatggttaa tcgtataagg aactgtaatg
     8041 acaattatta gtactaaaaa tccaaaacac aaggcatgaa atttatcaaa aaactccata
     8101 aaactatcct cctcaccaaa cagacttcct acgcgtcatt ctttagttct agcctttcca
     8161 aaacaaacca ttttatactc ttcgaaaatc aaattcaaac cacgtcaacg tcgccttgcc
     8221 gtactcaagt acagcctgcg gctagtttcc tagtttgctc tttgattttc attgagtatt
     8281 agtaaccaaa atccgaccac atagccagcc cctatgaata tagccattaa agctagcatg
     8341 gaatttagga aattaaaaac caccgcagat acaaaggtta gcacaaaaac attaaaagca
     8401 atggtgtcag aagccaagac tagaatatag ggtgtcaacc gatctaaagt tttggaatct
     8461 aggaaaaata agtgtttata catgataatc tcctctatgg ctgaaaagca agccttttgt
     8521 ttttttaccc caagacccta tgtaaaaaag tcagcaaaaa tggtaatgtt gcgaaaatat
     8581 tattgatcac atgcaccgca taggaaggat aaatgctctt ggtatagcgg gtcaaactag
     8641 caaagatgat gccagatgtt gcaaagatga agatatctaa caaactaggc aggcttgaaa
     8701 aatgaggaag agcaaataaa attgacggaa gaagcaaatc aaggccaaat ctcgaatgct
     8761 taaaaaaggc gtgttgcagt aatcctctat agattagctc ttccataagt ggagtcagaa
     8821 agaacaaagt tatatagata cctagctcag caaagttggt cccactataa ccgatcaata
     8881 cagcccaacc ttcagcagtt gactgaacat gtttagctgt ctgaacgtta aaagagatct
     8941 ggagcactac cactaatact gtcaaaatcg aataccaaag ccattttttt cttggaatgt
     9001 gaaagagata accatgacct gtcttaacca gaatccaaat catgactccg ctaaatagca
     9061 aactcaagaa attttgaatc cagacgaaat tgccaatctg ggaagaaaat tgccaataat
     9121 tttgaataat aagcgtcatc tgagaaagac caaatacgaa aaataagtaa gagaaaactg
     9181 cacttatttt gaatagaagt tgatactttt tcatagaaat cctccctact atgacctcac
     9241 cttgtcaggc tctactgctg taagattaag aagacagttt gtttttttta aggctaacct
     9301 gactactaga taatagatac attaaggcat taaagacaat gaaaatatgt ccatagaata
     9361 aaatcaacct cgcatccaaa ccaagataaa gtttgattat caaaaagatg agcaaaagaa
     9421 tttgaaacca taaggttttt ccaaaaataa atttaaagcg atttcgaata tctacttcct
     9481 tgatttttac cgccacccct ttattagcaa gaaggaaaac tcctgcttca aacaaaccac
     9541 tgtaaagaac aagccaccca atagatacga tagagatttg taaaaatgtc cctaaaagaa
     9601 tatccaacac actactcaag aaaataacaa aaaataatct gtatttcata ttaaatacct
     9661 ccattcattt atttcactaa caatttaata gagccttcta ctcaaatatc ctgtcagaaa
     9721 aggatagaaa gctacttttt ataatacttc aagccccaca tgagcagaag cgtgataaac
     9781 aagcagagaa tacacctata taagcgatta gttgttgata gaattctgtt tctgaaatac
     9841 ctctatacaa acaaatgaca aacataaaat ctgccaagcc gataaacata agttgattgg
     9901 ttctaggact aaccaaatca tcatttactt atatttaaga gtatctcttt tatgttaatg
     9961 tatgttagca ctgaaaagca agacaggcca ataatattta aaatgaacag taacggggtt
    10021 aagtctctaa aaaaattatc tactgacact acaagaaata ctatacatat tatagtcgaa
    10081 actatctttt tcttatccat aattatttac tcctttccta acaaatccaa cttatcaatc
    10141 aagagcgatt tttaacataa tgtagcagca cccgttgcaa ctttgacaag tttagtatat
    10201 cattgttttt taaaattttt catccaaatc ttgaattgtc atcgaaacct cctaaattgc
    10261 aaaaacttca ttagccccta ctttttataa tacttcaaac cccaaatgag tagaagcatg
    10321 atgatcaagc aaagaatagc accaatataa gcgattagtt gttgataaga ttctcctgct
    10381 gtgatacctc tatacaaaca aataatagac ataaagcctg tcaagccgat aaacatgagt
    10441 tgattggttc taggactaac caaatcatca tcttcaaact ctcttaatct catttcccta
    10501 gtaagataaa cagtaaccaa aatagaagtc aagttaataa ctactaaaag aaattggaaa
    10561 actgacgaaa tagaaataga taagtagaaa caagcaaagg caactgaccc aggaacaatc
    10621 tcacaaagaa tatgattctt tttaatcaag aaaatctttc atttcttttc tttcttttta
    10681 attaaagaaa aatagatcat aaccgcaatc atataggcta tggtataaaa tagattatac
    10741 caaatactct ccctaagcgg ataaagaaag atggacatga tcagatacag aacgaaaata
    10801 atcagtattt ttttcttcat aagttttcct cctaaatgtg cgatttatct tagttgagca
    10861 agaacattta cactgctagt atagcactta ttttgacctt ggatcactca aatcataaat
    10921 ggtcatcaaa acctcttgaa ttgtaaaaat taaaaaagca agcatgaaaa acatactttc
    10981 ctctttatat tgtattgata ccaacttgtt tgtagacttt tcatcctgct atcacatatc
    11041 attttgacag gcgaaacaat attaaagaaa ctcccctgta aattaagcta gcaaatacag
    11101 gggagaaatt tattttttag agagtactat ccgtatcctt tttggaagat tttgaaaata
    11161 tttttctaat taagtcatcc atataaggac caaatatacc aactactaaa ccaataataa
    11221 aacttttaaa atccataatt accaccaaca tgttgctgca taggctacac ctccaagtat
    11281 agctccacct gcagcaccag ttacacctat tcctatagca aatggtccca atagaaatgt
    11341 caaaccgttg ttgcacaccc atcaattgcg ccatatgcaa cccctgctgc acaactaatt
    11401 tttcttcccc aatcaatatc tccaccttca acgcaagcaa gcatttcatt atccataaca
    11461 gaaaattgtg acatcatttt tgtattcata tagtatgtca cttttcagtt acggaacaag
    11521 tttaatataa aaattatcaa aaaaacatag gcaataaaga gaaaaattaa tttatcatag
    11581 attagaaata atatgacaaa acaattcaat gatgttaatt caatagtctt ttgtttttta
    11641 tcagagatac ttatggatag ataaataaga taggtttgaa aagcgaagag aataataaag
    11701 aatatagcct tcataaaatt tagctttcat ttttatgatg tagcggtata ggctaaatat
    11761 ccacaaacca ctgctcctcc aattcctcct attgcagcgc cccatggtcc tagaattttc
    11821 ccatatttca ctccacccgc tgcacaacct aaagcagcaa ctacagctgc tcctccaaca
    11881 cctgctttgg caaaatctcc ccaattgcat ccgccacctt caacgcaagc aagcatttca
    11941 gtatccataa cagaaaattg tgacatcatt tttgtatcca tgacaaatac tcctttttta
    12001 aaaaactaaa ataaatcaga atagaatcct cataatttta ctataagtct taccaactta
    12061 gtcccaattt atcaccaacc atacctccta agcatgttaa tccaccccca attgcaccaa
    12121 tgtgtgctcc aacaaatgca ccagcaagtc cagctactcc taaagtggcc aaacctgctc
    12181 cagttccacc agttataatt cccgtagtga ctcctgtaat cagtgcattt tgacaatcag
    12241 tggagctata ccccccttca actttcgcaa gcatttcagt atccataacc tctaactgtg
    12301 acaacatttt tgtattcatg atgaatacct cctttttatt ttcaatttgt taccaaagtc
    12361 ttaaattcaa taaacaaata gatttttttt agtatctttt tgattttctt aaaaaagtat
    12421 atacgtctac tatcttctta aaggtagcag tacctatttt ttagtctaag atttcaataa
    12481 tcttgagtat ctaaaatatc ttaatttcgt tattctcctt gcaataaaaa gttttactat
    12541 actatttatt aacttgcaga aagcaaaaaa tattagtaaa taatagttta tagttaagtt
    12601 ttttattcct accaatccat caactaagta aagcatcaac gattacataa acgattgata
    12661 atataattaa aattttgcta actatcttat tctcatgatt cttagataac tttgatattt
    12721 tgtaagtaag taaataagac agtaaattaa tagcgataat aatactatat ttaagaatca
    12781 taatcttaca aagaggacat aattcctgaa cctacacaaa taagtgttgc tgctccccca
    12841 gttatcggac cagtcgcagc agctaatagt actgctccaa tacaaccacc gattgcagat
    12901 cctaaattgc ctcttcctcc actaactatt tcgagttctt cattatccat aacagaaaat
    12961 tgttccatca tttttgtatt catgacaaat actccttttt tcttttttta tttttgtctt
    13021 gttgtaactt tgataagttt agtatatcat cgttttttaa aatttttcat ccagatcttg
    13081 aattgtcatc gaaacgtctt gaattaggtt ttttatttca agccacctct aaatgtttaa
    13141 aaaaaataat ttctaatcac ttttttacca ttcaggaagt tttaatgact attcaagatt
    13201 tcataaaata tgaacttagt tttatgacat aatagaccta tccactatat gaaaggaatt
    13261 gccaatgact tcttataaac gtacatttgt tcctcaaata gatgcgagag actgtggtgt
    13321 cgctgcctta gcctcgattg ctaaattcta tggttcagat ttttctctag ctcacttgag
    13381 agaacttgca aagaccaata aagaagggac gactgctctt ggcattgtaa aagccgctga
    13441 tgaaatgggc tttgaaacaa gacctgttca agcagataaa acgctctttg acatgagtga
    13501 tgtcccctat ccatttatcg ttcacgttaa caaagaagga aaactccaac attactatgt
    13561 tgtctatcaa acaaagaaag actatctgat tattggtgat cctgaccctt ctgtaaaaat
    13621 cactaaaatg tcaaaagaac gctttttcta tgaatggact ggagtagcta tttttctagc
    13681 taccaaaccc agctatcaac cccataaaga taaaaagaat ggtctactaa gcaagcttcc
    13741 ttcctctgat tttcaaacaa aaatctctca ttgcttacat tgttctctca agcttattgg
    13801 tcactattat caatataggt ggttcttact atctccaagg aatcttggat gaatacattc
    13861 caaatcagat gaaatcaact ttaggaatca tctcagttgg tctggttatc acctatatcc
    13921 tccaacaagt catgagcttc tccagagatt atctcctaac cgttctgagt cagagattaa
    13981 gtattgatgt gattttatcc tatattcgcc atatttttga acttcccatg tctttctttg
    14041 cgacacgtcg tacaggagaa atcatttcac gattcacaga tgctaactct attatagatg
    14101 ccttggcttc taccattctt tctctttttc tggatgtttc tattctgatt cttgtaggag
    14161 gcgtcttact ggcacaaaac cctaatctct tccttctttc tcttatttcc attcctatat
    14221 acatgttcat catcttttct tttatgaaac ctttcgaaaa aatgaaccat gatgtcatgc
    14281 aaagtaattc tatggttagc tctgccatta tcgaagatat caacgggatt gaaactataa
    14341 agtcgctcac gagtgaagaa aatcgctatc aaaatataga cagcgaattt gtagattatt
    14401 tggaaaaatc ctttaagctc agtaaatatt ctattttaca aacgagttta aagcagggaa
    14461 caaaattagt tctgaatatc cttatcctat ggtttggcgc tcaattagtc atgtcgagta
    14521 aaatttctat cggtcagctg attaccttta acacactttt ttcttacttt acaactccta
    14581 tggaaaatat tatcaacctc caaaccaaac tccaatctgc gaaggtcgct aataaccgtt
    14641 tgaacgaagt ctatctagtc gaatctgaat ttcaagttca agaaaaccct gtccattcac
    14701 attttttgat gggcgatatt gaatttgatg acctttctta taagtatggt tttggacgag
    14761 ataccttaac agatattaat ctcacgatta aacaaggaga taaggttagc ctagttggag
    14821 ttagtggttc tggtaaaaca actttagcca aaatgattgt caatttcttt gaaccctaca
    14881 aaggacatat ttctatcaat catcaggata ttaaaaacat tgataaaaaa gtcttgcgcc
    14941 gtcatattaa ttacctacca caacaagcct atatctttaa tggctctatc ttggaaaact
    15001 taactttggg cggtaatcat atgattagcc aagaagatat tctaagagct tgtgaattag
    15061 ctgaaattcg tcaagacatt gaaagaatgc ctatgggcta tcaaactcag ctctctgatg
    15121 gagctggtct atcaggagga caaaagcaac gaatagccct cgctcgtgct cttttaacta
    15181 aagctcctgt tttaatacta gacgaagcta ctagcggtct tgatgtcttg actgagaaaa
    15241 aggttataga taatcttatg tctctaactg ataaaaccat tctctttgta gcccatcgtc
    15301 tcagtatagc cgaacgaacc aaccgtgtca ttgttcttga ccaggggaaa atcattgaag
    15361 ttggtagtca ccaagagtta atgcaggcgc aaggtttcta ccatcatcta ttcaataaat
    15421 aaggagaatg tcatgaatcc taatcttttt agaagcgtcg agttttatca gagacgttac
    15481 cataactatg cgacagtgtt cattatacct ctttcattac tatttacttt catcttgatt
    15541 ttctcccttg ttgccacaaa agaaattact gttacttccc aaggagaaat cgcccctaca
    15601 agtgtcattg cctccattca gtcaaccagt gataatccta tcctagctaa tcatttagtg
    15661 gcaaatcaag tagttgaaaa aggggactta ctcatcaaat actctgaaac aatggaagaa
    15721 agtcagaaaa ctgccttagc aactcaatta caaagacttg agaagcaaaa agaaggactt
    15781 ggaattttga aacaaagctt agaaaaagcg actgatcttt tttctggcga ggatgaattt
    15841 ggctaccata atacctttat gaattttact aaacaatccc atgatattga actgggtatc
    15901 acaaagacta acaccgaagt ttcaaatcaa gctaatcttt ccaatagcag ttcatcagct
    15961 attgaacaag aaattacaaa agttcaacaa caaattggag aatatcaaga gttgagagat
    16021 gctatcataa ataacagagc acgcttacca actggcaatc cgcaccagtc aattttgaat
    16081 cgttatcttg tagcctcaca aggacaaaca caaggaactg cagaggagcc atttttatct
    16141 caaattaatc aaagtattgc aggtcttgaa tcatctatcg caagcctcaa aattcagcaa
    16201 gctggtatcg gaagtgtagc aacttatgat aacagtttag caaccaaaat tgaagtactc
    16261 cgcactcagt ttttacagac agcctcacag caacaactaa ctgtggagaa tcaattaaca
    16321 gaattaaaag tacaactaga tcaagccaca cagcgtttgg aaaacaatac cttaacctcc
    16381 ccaagtaaag gtatcgttca tctgaacagc gaatttgaag gtaaaaatag aattccaact
    16441 ggtacagaaa ttgctcaaat attccctgtc atcacagata caagagaagt actaatcact
    16501 tactacgtat cttctgacta tctacctcta ctagataaag gacaaactgt aagattaaaa
    16561 ctggagaaga ttggaaatca cggcaccacc atcatcggcc aacttcagac aattgatcaa
    16621 actcctacca gaacagagca aggaaatctc tttaaattaa ccgctcttgc aaaactatct
    16681 aacgaggata gtaaactcat ccaatatggc ttacaaggtc gcgtcactag tgtaactaca
    16741 aagaaaacat attttgatta tttcaaagat aaaattttaa cccattctga ttaattttca
    16801 gataacactc tataactatt tattatctta tcaaaaagga gaatcataac atggataaga
    16861 aacaaaacct aacttcattt caagaactaa caactaccga actcaaccaa attacaggtg
    16921 gaggattgtg ggaagattta ttatataaca ttaatagata tgctcattac atcacataag
    16981 aacttcatca tccaatacaa ctataaaaaa ataagaccga gaaacaagta ctctcggtct
    17041 tatttttcat cattctgtat gtatcacagt aagtacctga cgaaagactt gattttgaca
    17101 ggtggtattt agactggtat taggatggct ttccacaatc ttcatgacgg tatagagacc
    17161 aactcctctc tcctcccctt tagaactggc tccaaaggag aagatttcag aaatatcgat
    17221 gccctcttct ttgatggagt tttcgatgat aaaggtctcc tgtgctccat tttttaaaaa
    17281 ggcgattgaa acatgaggtt gactagcttc cacactggct tcaatagcat tgtcacaaag
    17341 gatagacaca atggttagaa aatcaagtag actcatcccc tcgacctgaa tctcctcagg
    17401 aacttcgaca ttaaagacaa tgttcttatc tctggctttt aaaaatttcc ctgctagaag
    17461 acttttgagg gctttatcac gaatatttac caatctgccc aggtcatatt tattgttctg
    17521 caatttctga ctggaatcct ttaagacgga gccatagacc tcttttatct gctccatatc
    17581 ctcctcttca atgcccagac gtaagctagt caagaggttg gtataatcat gacgaaagct
    17641 ccgtacttcc ttgtaaagct cctctatatg ccgactatag cgttccatat ctctatagcg
    17701 cagggcctgc tcttgttcca atctctcata gagtttttcc ttcaaatagg tatccaattt
    17761 cttgataacc cccataaaaa agagtaggta aaagactagg atgagatggc gaacagtctt
    17821 tgattgaata ctttgttcat attcaaaaaa agacagactt tccatgacta gatagtagcc
    17881 acccattatc cagttaatct gagtcaggga cttttgaaag gctttatcga gaatctcctt
    17941 tctcaagcta gtaaaatcgt agtccaacca tttcaaaaaa gctagagaaa tgaagaaatt
    18001 gaaaattatt atacataacc cagtaaatga gtagccatca tatacttgcc cttgtcccaa
    18061 aaatggaagc acaaaatagg agactcctct ataaaagaga ttcaccaata tcattggaaa
    18121 gagaccataa aagaaaagga gttttttagg aagccctctc aataataaga aagataagcc
    18181 tatgccgtac aagggttcca taaaataaga taggtaaaca tttcctacta tatagctaat
    18241 catcacaaaa acaaaggcca acagtatctt aaaaagaaag gccttaaaaa tcctctcgaa
    18301 agtaagatca attccatcca ccttaaagaa gatgacaatt tctagtccat tagtaacaag
    18361 tgtatacaac aatatccaag caatgttcat aaattctcct agctcagtgt aatttattga
    18421 tggcctcaga cacttccctg accttataac gggcgattag acaacttcca ccattgggaa
    18481 agaaaagcag tttttctttc ttatccaaat gcaccacatt tgcaggattg atgagaaaag
    18541 agcggtggca ctgcaagaga cggggctcct gcttgaaaac ctcctctaaa ctcgctgtaa
    18601 attccagcct gtctgtcttg gtatagagaa taacacgatg ggctctgggc gacgtttcga
    18661 gatagtaaac ctctttaaaa ggatactgaa attgggcaaa ttttgattta aagtaaaagc
    18721 aatcttccgc cagactttta ctatcttgac tattggcata gaggagggct gtctcgatcc
    18781 gagattcaaa ctcctctgct gacaaggcct tatcaatgta gtccaaagca gacacttggt
    18841 agcgaaaaga caggggcata aactccgagt gagtcgtcac aaagacaatc agggcataag
    18901 gatcccgatc ccgaatcttt ctagccactt ccagtccctt catctcttca tttcgaatct
    18961 caatatccaa aaagaatagc tgatgggccc ccttctcatg cacttcagcc agcagttggt
    19021 ccggcttgcc aaatacctca aaagagctag gaatgatatg atgtgctttc aaaagtttct
    19081 caatcgtcgt ttcaattcta gtctgttggg aaaaatcatc ctctaaaaca aatattctca
    19141 tcttcttact ctccctcttt caaccatttt tgacgaattt ctctatagcg acgtctggca
    19201 tacttgacag cgtattgacc ttcttctccg agaaaaagga ttctttcttg aaaatcaatc
    19261 gattttaatt tatcaagatt aaccaaacaa tttcgatgac atctcatcaa ggtttcccca
    19321 cattggttct caagattact taaattttga agcatattaa aactagcttc ttctgtaaca
    19381 atctgtacgg tatgggcttt agttggatgt gtttggatat agtagatatc gtcgatgttt
    19441 actttataga ctgttttctg cgtctgaata atcatatatt tcattggatt ctcctcctaa
    19501 gaaaagaata acagaaaagg tctagcccaa gcacacccaa tcctaaacag tctttgaaaa
    19561 ctcctaaaag gattattttc tacaacatca tgatgagctt acaactattt tatcataaaa
    19621 ttcactcagc gcaattcaag acatttcaat gacaattaaa gatttgaata aaaaaaaaga
    19681 attaaaagtg ataaactaac agtatcaaaa cacagttgcc agaagtaaga ctggcaccca
    19741 gattaaagga ggcattccca tgacagatac agaccccatc aaaagagctc atactttgat
    19801 tactgactta aacaaagcct accaagcatg caaacaggca agcgctgacg acgtccgctt
    19861 tcaggagcaa ttaaactcta ttcttggttt tctagccaag gctgaaacag tggataatcg
    19921 attcttgatt gaactagaaa aattttacca gacttccagt cttctcatgg gactcagcgc
    19981 tcttgatcca gatgctccaa ctcgcgccgc ttggcgggcc tatgaccgct tccactttga
    20041 ccaagtcaag accaagttaa tactcaatga aaatcaaaga gcaaactagg aagctagccg
    20101 caagctgtac ttgagtacgg taagacgacg ctgacgtggt ttgaagagat tttcgaagag
    20161 tataagtctc tacggaccaa ccattatcct gtagtaacaa aaaagaagtt gagacagact
    20221 gaactcaact tcttttttag tgtcgtatca gcaatgttag tcctgcatct gacgttttac
    20281 ctgataaggc aaatacaaga ctagtaaaac caaaccagct cctagtaagg ctagaagggc
    20341 tagtttttct tggaaggtaa tcaacccaac aactaattcc acaagtaaaa caaaactggt
    20401 caatcctcga actacctcct gtaagccttt ttccttttgc cccttgtcca gcggaaagag
    20461 ttgggtcaaa tactggtagt caaaggcatg atagagggcc agcaactgga agagcaagag
    20521 gtagttaaag agaactacca ctgctgtcgc aatccaagct tgctcgataa aaacctgcgc
    20581 cagtacggaa agtaagagaa gacgaagact gagagcaaag aggtcgccat ttcgcagata
    20641 agaacgcaga tagagatttt gccaaatctt tccagacacc ttctgaacag cctttaagat
    20701 aaagtccaga taggcacgac gtttgacact gtttgaaatt ccctttacct gcgtaaagag
    20761 ggcaaagaaa cgaagcaaga cttgcttacg cttgctttct tgggaaatga catagtccca
    20821 gtccagccca gtttcagtga aaaatttgct ggccttttga cgaaagtgga aatattttcc
    20881 tacccccaat aaaagcacat agagcagaaa aactggcaag ccataaccca ttgctaaaaa
    20941 taaaggcgca aataacagca agaaaagggt ctgtacaaag agccaaaaga ctagggaaat
    21001 gccagtttga cgcttgagat ggagcttgat ttcctcttct ccaactaaga gaaagagctt
    21061 gtctggagcc tccatatagg tggcaattcc tccccaaagt aaaagtaaaa cagacgtaat
    21121 tcctacaaac aaaaggatag gccaatgatt ttcaggaaaa tgttgtaaga gttgactgta
    21181 ctggtaggct agaaagccca acaggacaag caggaacaag acaaagtggt cattgagcac
    21241 atagcgcaga taaccaaggc actccttacg aaaggcctgc tttctcttta aaaacaagtc
    21301 tttcatagat cctcctcttt ggtcagagcc aagtaaatat cattcaaact agcctcaggc
    21361 atatcaaagg cttcacgtag ttgcaggaga ttgcctttgg cacgcacctc tcccttgtga
    21421 agaatgacaa aggcatcaca catcttctcc gccgaatcca gcacgtgggt actcatgaga
    21481 atagacttgc ccttttgctt ctccacttcc aaaagctgaa tcaaatcaga aatagccagc
    21541 ggatcaagac caaggaaagg ctcatccacg atgaaaagac ttggatccac cacaaaagca
    21601 cagataatca tgaccttctg cttcatccct tttgaaaaat gaacagggaa ccagtctaat
    21661 ttctgttcca aacggaacat ttttaacaag ggctctactc gttcgaaagc cactttttgc
    21721 tcaataccgt aagccatagc aaccgtttcg atatgctctc tgagggtcaa ttcctcatac
    21781 agactaggcg tctcaggaat gtagccaatc tgcttgcggt agctagtcgc atctccttgc
    21841 agagtcaggc cattgatatt gatggagcca ctataaggtg ccaacagacc gataatctca
    21901 ttgatcgtcg ttgatttccc agcaccattg agaccaatca aaccgaccaa ctgcccactt
    21961 tcaacagtaa aggacacatc tttcaaaaca gggacatgaa catagccacc tgtcaggttt
    22021 ttaatttcta acatattttc tccaaatctg gtataatgta gctatattat atcaaaattc
    22081 agtacagtag aggtagattt tatgtcagat tgcatttttt gtaaaatcat cgcaggggaa
    22141 attcctgctt caaaagtata tgaagatgag caggtccttg cctttcttga tatctctcaa
    22201 gtaacactag gacacacctt ggtcgtgcca aaagaacact atcgcaatct tttggagatg
    22261 gatgctacga gcgccagcca actctttgcc caagtaccaa aagtagctca aaaagtcatg
    22321 aaagtcacta aggctgctgg tatgaatatc atttccaact gtgaagaagt cgctggtcaa
    22381 acagtttttc atactcacgt tcaccttgta cctcgctaca gtgctgacga tgacctcaag
    22441 attgatttta tcgcccacga accagacttt gacaaacttg ctcaagtcgc tgaaaccatc
    22501 aaaaacgctt aaggagttgc tatgaaatta tccaacctac tgctatttgc aggagctgca
    22561 gccggaagtt atctggtcac aaaaaatcgc caaaccatca cagatgaagt cttgaatacc
    22621 actgaccgcg ttcaagctat caaggacgat gtggatatta tccaaaacag cctgcaaatc
    22681 attgaccagc aaaaagaact tatcaaggaa taccaagaag acttgactta caagtttaag
    22741 gtcttggaaa aggatatcca gactagacta gctgtgataa aagaaatgca gggaactgaa
    22801 gataagtaaa aagagccccc cggctctttt ttaaaacgta aacaagttat agtgaattga
    22861 atttagaata gcacatcata gtttctaaag cattgttaga gattacttta aaatcctctt
    22921 atcaatttgt tcatattcta tttcaatcta ctatatatat cccatcaact atgcatcata
    22981 atttaggtaa ctcatactca ataaaaatca aaaagcaaac tagaaagcta ggcacagact
    23041 gctcaaaaca ccgttttaag gttgtggata gaactgacga agtcagctca aaacactgtt
    23101 ttgaggttgt agatacgact gacgaagtca gctcaaaaca ctgttttgag gttgtggata
    23161 gaactgacga agtcagtaac catacccatg acaagccgac gctgacgtgg tttgaagaaa
    23221 tttttgaaga gtatcattca ccatttcaca actaaatcga agactttctg ccgtaatgaa
    23281 aacacctgaa acagcttggt ttcggctgtc cggaaacttt aagacttagg ttcaaagttt
    23341 aggtatggaa cttcgaagga ggtcgctacc gtccgtcatt acttaggaaa gtcttaaaaa
    23401 atcggctcta taaagtaaaa agagcccgac agctcttttt gtttatattc aatgaaaatc
    23461 aaaaagcaaa ctaggaaact agccacgggc tgctcaaaac actgttttga ggttgtagat
    23521 acgacaagcc gacgctgacg tggtttgaag aaatttttga agagtattat tctccatcaa
    23581 aggcatcttt aatatggtca aagaagcctt ttttctttgg atttactttc aagtcaccag
    23641 cagccgcgaa ttctttcaag gctacttttt ggcggtcgtt caagcctgtc ggtgttacga
    23701 cattaacagt aacgtattgg tcaccaactg caccgccacg aaggctcggt gcccccttac
    23761 tacgtaggcg gaatttctta ccagtctgag ttccctctgg aataaccaat tcaacatcac
    23821 cgtgaacagt tggaatatct actgtatcac caagagccgc ttggacaaag ttgaggttga
    23881 gattgtagaa gatagtcgtt ccttcacgtt caaacttgtc gctagcttct acagaaacta
    23941 ctacatacaa gtcaccataa ggtccaccgt taaagcctgc ttcaccttga ccagcgaggc
    24001 gaatttgttg acctgtttcc acaccagcag ggattttcac atgtacgcta tgagcttgtt
    24061 tctcatgacc tgttccatga caggttgtac atggatattt gatttctttt cctcgaccgt
    24121 gacagacatc acaggttact tggcgacgca tcataccaag aggagtctgc gtatcgacgt
    24181 taatgacacc agcgccatga cagcgtccac aagtgactgg acttgtccct ggcttagcac
    24241 cagagccatt acatgtacga cagccagctt cacgatgata cttaacttcc ttctcagttc
    24301 cgaagatagc ttcttcaaag gtcaaattga cacgatactg gagatcatct ccttggcgag
    24361 gagcgtttgg attgcgcgaa gaaccgcctc cgccgaagaa acttgagaaa atatcctcaa
    24421 aaccaccgaa accacctgcc ccattgaaac cgccgaaacc accagctcca ccaaaaccac
    24481 cattggcgcc tgcagcacca tactggtcat aggcagcacg tttttggtcg tcactcaaag
    24541 tctcataggc ttcttgaact tccttgtact tgtcctcagc accaggctcc ttgttgatat
    24601 ctgggtgata ttttttggaa agcttacgat aagccttttt gatttcgtct gccgaagcgt
    24661 ttttggatac ccccagacga tcataaaatt cagtattgtt cataattcaa gatacaaagg
    24721 gcgtcaaacg gacacagcca tctaggagtt ttgacgacgg acgcttacgt cctagaaaaa
    24781 actatctttt gggcacagtc cgtagcccgt gttcagttac gaacaccctt attcctttct
    24841 attgatgttc tgaatcgaac caccatttag gtcgggttca attcctttct tttatattta
    24901 gtaagaaatt ctgaaacccg tgttcagtta cgaacactct ttgttccctt ctattgatga
    24961 gacaaaaccc aaacaaatga gattccgtcc ctttttatcg aaaaacagag gctgggttgc
    25021 aacctctgca aataagtaaa ttaactttat tttgatttta aatatcctat aagtacagaa
    25081 atatttatat atttttgtct cttcttagta tttgcctata taattccaat tgatcataag
    25141 aaatacaact actgtccaaa tcataccaat tatgaacggt attacaaatt tatatgtttg
    25201 tatttctaat gatatcatag ctggtaccat cgccgataca aatgcagaca acaatactga
    25261 ggtaacaaaa ttccttaccg cttcatcatt tttgatacaa ctatcatgtt cagctaatat
    25321 aatttctcta tatctagcta aatattctat tggaatatta tcaataaaat cttttgatgt
    25381 tctatagtta tcagcttgat tacaaaaatc attataaact tttcgtgtat tttttagata
    25441 ctcttcatcc aatacactca tcttactttt ccgtaaactc tccgtctacg acgtcatcgc
    25501 ctgcgttccc tgttgcttgt gcgccttctg ctccttcttg agcttgttgc gctgctgcgg
    25561 cttgttcgta gagtttaaca gcaagtcctt gagctttttc gttcaatgct tcaagttttg
    25621 ttttcatgtc gtccaagttg ttgtcttctt gagctttctt aaggtcatca agggcagctt
    25681 gggcagcgtc acgttctgcg tcgaagcctt taccttcagt ttccttgatt gtcttttcag
    25741 tcgcaaagat tgcttggtct acttcattac gaaggtctac ttcttcttta cgtttcttat
    25801 cggcttcagc gtttgcttct gcatctttca tcatgcggtc gatttcttcg tcagtcaaac
    25861 ctgagttcga ttggatgaca atagtttgtt ctttttgagt tccaaggtct ttggccttaa
    25921 cagacacgat accgttcttg tcgatgtcaa atgttacttc gatttgagga attccacgag
    25981 gtgcagctgg gatatcagtc aattggaagc gtccaagagt cttgttatct gctgccattg
    26041 ggcgttcacc ttgaagaacg tggatatcaa cggctggttg gttgtctgct gctgttgaga
    26101 agacttgtga tttagatgtt gggattgtag tgttgcgatc gataagtttt gtaaatactc
    26161 cacccattgt ttcgatacca agtgacaatg gcgttacatc aagaaggaca acgtccttga
    26221 catcaccagt aatcacacca ccttggatag ccgcacccat agcaactact tcatcagggt
    26281 ttactgattt gtttggttct ttaccagttt cagctttaac agcttcaaca acggcaggga
    26341 tacgagttga accaccaaca aggataactt cgtcgatttc tgacaagctc aaacctgcat
    26401 ctgaaagggc ttgacgaact ggaacttttg tacgttcaac aaggtcacga gtcaaatcgt
    26461 caaatttcgc acgagtcaaa gtcatttcca agtgaagagg tccagcctca cctgcagtga
    26521 taaatggcaa gctgatttgt gttgaagtta caccagaaag gtctttcttc gctttttcag
    26581 ccgcatcttt caaacgttgc attgccatct tgtcagtaga caagtcgata ccgttttctt
    26641 tcttgaattc tgctaccaag tggtcaatga ttttttggtc aaagtcgtca ccaccaagtt
    26701 tgttgtcccc tgcagttgac aatacgtcga agacaccgtc acccaattca aggatagaga
    26761 cgtcgaatgt accaccacca aggtcaaata ccaagatttt ttcttctttg tcagtcttgt
    26821 ccaaaccata agcaagagct gctgcagttg gttcgttaac aatacgttct acttcaagac
    26881 cagcaatttt accagcgtct tttgttgctt gacgttgagc gtcgttgaag taagccggaa
    26941 ctgtgataac agctttggtt actttctcac caaggtagtc ttcagcgtag cctttcaagt
    27001 attgaaggat catagctgag atttcttgtg gagtgtattc ttttccattt gcagaaactt
    27061 tttcagaagt tcccatctta gatttgatag agataactgt atctgggttt gtaactgctt
    27121 gacgttttgc agcatcacca acgatgattt ctccgttttt gaatgagact acagatggag
    27181 ttgtgcggtt tccttctggg tttgcgatga ttttgctttc agttccttca agaactgcaa
    27241 ctgctgagtt tgttgtacct aagtcaatac cgataatttt agacatgtgt ttttctcctt
    27301 gttagttaat tttctatttt tctttttgat actcttctta agtggcgacg ccgtcagagc
    27361 ttgacttcgt cagtctcttt tactaaactt tgagcctaag gtctcaaaat ttgcgcaaat
    27421 agcgccacgg cgaagagtat cgtctttggt tcgcttcgct cactattaca aagcaaaaca
    27481 tattttatct ttcttcatag tttattgtcg tttcggacaa gttttctatt atgtaaattg
    27541 cgacacgagg agtcgaaatc gattttattt cgacgacgag ttagtaagga agctaggcaa
    27601 acgccatagc aattgccgtt ttctgacgag ttgctttagc taccgtcaga attgccgaat
    27661 cgaacacgcc ctaagctctg agtaaaaaag atagattttc ttgtgttcat cgaacacttc
    27721 gtcaatctcc tatttttact gcgagctttt tacgggcttt gtatcttagt tatacaccac
    27781 taccattgct gggcgtagga tgcggtcatg gagtttgtag cctttttgaa agacttgggc
    27841 gatggtatct actgggtgtt catcgtctgc tgggagagtt tggatggcca tatggtagtt
    27901 atggtcaaat tcgccatctg ctgcgatttc ttcaattcct tcttctttca aagcgtgaat
    27961 caagctttct tgcaccatcc ccaagccctt cttcacatca tctgtcaaac cttcaactgc
    28021 aagtgcacgc tcaaggttgt caagagatgg taagattgct tttgccaagt cctggctacg
    28081 ataacgttgc aagttttgac gttcttcatt ggcacggcgt tggatatttt gcatttctgc
    28141 atgagcgcga agatatttgt tttcgaactc atctgcacgt tcatttgcca agtccaactc
    28201 agacttttca ggagttgttt cttcagctgt ttccacaact tcctcttctt gaacttcttc
    28261 tacttcttca ttttttatat cttgggccat gttcgcctcc tttaatgatt tcaatcttaa
    28321 tgtacttcgt aatgattact gctgaggtag cggtaaaaat ctgtcaactt catggtcaaa
    28381 acacggttga ccacattgat ttgattgatt agctgttggt aatccagatt aactggaccg
    28441 ataatggcta gaattccaac tccccgataa ggaatgagga acttactact aatcaccgct
    28501 aggtcagcta aacaggactc ttgaccgtct gcaacacgaa cattttgcat ctgatcctca
    28561 cgcaacccct cacgaatctc caaggccacc ttttgcggtt ggtcaaagaa ctgataggct
    28621 gctagattgg caaaattcaa gagattgacc ttgcccgcca tcacaatgtt ttcgttgaac
    28681 atttccttaa agatgtgttc aaagagatcg atgacattat ccgttgttgt aaagtaacgc
    28741 tggataatct gcggaatctc cgtccgaatc ttgtagtgaa tatctaaaac ggtgtgaccg
    28801 aggaaacgtt cctgaatgat gctcttcagt ttcagcaaat cctcctgcaa gaagttcctt
    28861 ggaatcagaa actgactagt aaccgttcgc gactcgtcta gggtaaatac cgccaaggct
    28921 gtatgttgcc ccaaaacaac gatatcaaag gctgtcaaac gttgcctgct cggctcaaca
    28981 tccagtgcca ctaccgtaca gccactcagg tctgttagca agttagcagc ctcttgcaga
    29041 atatcctcca atttgaagaa ttcctgatca aaggctttga caatctcata tacctcattt
    29101 tcagccagtc ggtcaaaatc cagtgagtgt ttcacatagt actgaaaacc agcaacactt
    29161 ggcatccgac cgcttgaagt atgagccttc tcaagcaacc cttgtttttc tagttccgcc
    29221 atgtcattac gaatggttgc actgctagag ttaatagact cttgcaaggc ttttgatccg
    29281 acaggttcgt gcgttttggt aaagatgtca ataatcagat ttaaaatatc ctgctgacgc
    29341 tctgtaacca tctcatcact cctctctgtc tgatcaatta gcactcaatg catccaagtg
    29401 ctaacttatg attctattat acacccttag aagagaatgt caagacaaaa actcaaaaaa
    29461 ttagcactct tttataaaga gtgctaaaaa tcaatctgaa gacctagaac cttgctattc
    29521 atctactcgg tatttccttt ttagtctgaa ataaccatga attagataag aaatcagaaa
    29581 agcatataaa acaaaaataa gaaagaaaga catagaaaga tcctctctaa acaaactcat
    29641 acaaacaaca agaccaccta agaaagtagc tatacaggaa cgaagtctag atcgcataac
    29701 ttcccatttg agccccttac tcatatagac ttgatcctct ggaagtaaat cagaagacga
    29761 tttacgaaga atcgaacaag aacctgctcc tatcaattcc caacctctat ctctaaaatc
    29821 ttgcagttca cgcttatgct ctttaagaaa tctagaatca tagatacgat agataacatc
    29881 gtccggttga ccttgctcaa aatagaacaa accaaaacga ctagttctat acctccaacc
    29941 tttcaaatgc atctcatgta aatattcttc tttcttgtcc aaatcaacaa tggtgaaaat
    30001 ccgaaattct actctgctat tcattgtctt accccaaaat tagaaaacat gcctagcgtt
    30061 atttattaga taattctttc cacttttgac tcaatctcca aaaaatataa gaaatctgaa
    30121 tcgcaaaaac tatcaataaa acccaatcta ttatgaaaat caaaaacact tcccaactga
    30181 aagaactacc tccagtgaca aactttgaga aaaacggtag tagagctaaa aagagaagca
    30241 aaataggaag catccgcatt gttaaaatcc ttttggcata aaaaaatctt tatttaaact
    30301 aaaatattat ggcaaaattt acgccagttt ttgaacggct gatgtagata ttttatactt
    30361 tcaaaatgtt taaatgtgat tatttatttt tgaaaaatag ctcaccagcc cgactgaaag
    30421 tgcttataga atgataataa gtcgcctgcc gaaaacagcg aaaaatagca gtgttatgcg
    30481 gagataatct gacgcgatgc gaaagtatat tgcatactta ttttcaacaa tttagcagag
    30541 tatttttata agtgtgatat aatagaagta taatttgttc tgatagttta ttttatggag
    30601 aagtagattt ttagaatgcg gaggattcaa tatggttgag tttataaagt ctaagaaaga
    30661 aatgagtgag gaggatatta aagcaaattt catcactcct gctattgtat ccaaaggatg
    30721 gaaaaatggt gagcatatcg cttacgaaga atacttcact gatggtcgaa ttgaagttag
    30781 aggagataag gctcgtcgta aagaaggaaa aaaatcagac tattcactgt attaccaatt
    30841 tggaactcga attgcaattg ttgaggcaaa ggataataaa cacagcgttc gagcaggatt
    30901 acaacaagct attgaatatg gagagatttt agatgttcca tttgtttatt cttcgaatgg
    30961 tgatggcttt attgaacacg accgtatcac gagagaagaa cgtgagctgg agttagacga
    31021 attccctact cgtgaagaat tattttctcg tatgacgaag gaaaaaggat tgacgtacga
    31081 aattacagaa gctatctcaa ctccatacta tacagacgcc ttctcaatga aaacgccacg
    31141 ctattatcag caaatagcta tcaaccgtac tattgaaaca gttgccagag gacaaaaacg
    31201 agtaatgttt gtgatggcaa caggaacggg gaaaacgttc atggcttttc aaattattca
    31261 tcgccttcga aaagctggtt tggctaaacg agttttattc ttagcggata gaaacatctt
    31321 agtagaccaa acgatggctg aagactttaa gccattcgaa aaggtaatga cgaaaattac
    31381 accaaaactt ttgactgctc ctgaaaaatt aaattctttt gaaatttatc tagggcttta
    31441 tcagcaacta actggtgaag atggaactga aacacattat caaaaatttg ataaagactt
    31501 ctttgattta atcgtaattg atgaagcgca ccgtggttca gctaaggaaa acagtaactg
    31561 gcgtaagata attgattatt tcagttctgc gacacagatt gggatgaccg ctactcctaa
    31621 agaaaccaag aatgcttcca atacggaata ctttggtgag ccaatctata cttatagttt
    31681 aaaacaggga atcgaggatg gttttttggc tccatatcgt gttatgaggg ttaatttaga
    31741 tgtggatgtg gatggttatc gtccagaaac tggaaaagtt gatactaacg gacaattaat
    31801 agaagatagg tactacggca ggaaagattt tgataaaacc attgtcattg atgatagaac
    31861 gcaaagagtt gccaagtttg tttctgatta tatgaagcaa aacaatgcac gatttgataa
    31921 aacaattgtt ttttgtgttg atattgacca tgccgagcga atgcgtgctg cacttgtaaa
    31981 agagaatcta gacttagtcc aagaagacta tcgttatgtc atgcaagtaa ctggtgacaa
    32041 cgctgaagga aaagctcaac tggataactt tatggatgtc aattctaatt ttcccgctat
    32101 ggtaacaacg tctaaattat taacgacagg agttaatgct aaaacatgtc gtttgattgt
    32161 tttagactct aatatccaat ccatgactga atttaaacaa attattggtc gtggcacacg
    32221 tctttatcct caaaagggga aagaattttt tacgattatt gattttcgaa atgttaccaa
    32281 tttgtttgct gaccctgatt ttgatggtga gccagtgaag gtgctggaaa caggtgcgaa
    32341 aacagtcagt ggttccacgc ccggtttcgt agatgaggaa ggtgacccag tagaaaaata
    32401 tatcgttaca gacaagcagg ttaccattct taattctact gttcaagtat tggatgaaaa
    32461 cgggaaactg attaccgaaa gcctgaccga ctacactcgg aagaatatct taggtagcta
    32521 cgccactttg aacgatttta tcacagtttg gcatacggcg gataaaaaga agcttatctt
    32581 agacgaactt tataaaaaag gagtttatct agatgctatt cgagagtcgg agggaatatc
    32641 agaacaagaa atcgatgatt ttgatttact cctaaaactt gcctatggtc aaaaagaatt
    32701 aaccaaaacg gaacgtatca ataagctcaa acaaagcgga tatttatata aatatagtga
    32761 ggaagcgcgt gctgttttgg aaattttact gaacaaatac atggataaag gtattggaga
    32821 actcgaaagc attgaaacat taaaacttcc agaatttcag atatatggtg gaaccttcaa
    32881 aatcatcaat acttattttg gagataaaaa acgatattta caagcaatta aagaattgga
    32941 gcaagagcta tttacagtag cttaatgaaa ggaaagtatg tcaattacat catttgtaaa
    33001 aagaattcaa gatatcactc gaaacgatgc tggtgttaat ggtgatgctc aacgtattga
    33061 gcaaatgtct tggttattat tcttaaaaat ttatgatagc cgtgaaatgg tttgggaatt
    33121 agaagaagac gagtatgagt caattatccc agaggaatta aaatggcgaa attgggctca
    33181 tgctcaaaat ggggaacggg tattgacagg cgatgaatta cttgattttg tcaataacaa
    33241 gttattcaaa gagttgaaag agcttgaaat aacttcaatt atgcctattc gaaaaacgat
    33301 tgttaaatca gcttttgaag atgcgaacaa ctatatgaaa aatggcgtct tgttacgcca
    33361 agtcatcaat gttattgatg aagttgattt caatagccct gaagatcgtc attcgtttaa
    33421 tgatatttac gaaaaaattc ttaaagatat tcaaaatgct gggaactcag gagaatttta
    33481 tacgccacgt gcagcgactg attttattgc cgaagttctt aacccaaaac ttggagaatc
    33541 aatggcagac cttgcttgcg gaacaggagg cttcttgact tcgactctga accgtttaag
    33601 tagtcaacgt aaaactagtg aagataccaa aaaatataat acagctgttt ttggtattga
    33661 aaagaaagca tttcctcatc ttttagcagt tacaaatctg tttcttcacg aaattgatga
    33721 ccctaaaatt gttcatggaa atactttgga gaaaaatgtt cgtgaatata cggatgatga
    33781 aaaatttgac attattatga tgaatccacc ttttggaggg tcagaattag aaacaataaa
    33841 aaataacttt ccagcagaat tacggagttc tgaaacagct gatttattta tggctgtcat
    33901 tatgtatcgt ttgaaagaaa atggtcgtgt tggagttatt ttacctgatg gttttctatt
    33961 tggtgaaggt gtaaaaactc gcttgaaaca aaaactggta gatgagttca acttgcatac
    34021 gattattagg ttgcctcata gtgtctttgc accgtataca ggaatccata cgaacattct
    34081 tttctttgat aaaacaaaga aaacagaaga aacttggttt tatcgtttag atatgccaga
    34141 tggttataaa aatttctcga aaactaagcc gatgaagtca gaacacttca atcctgttcg
    34201 tgactggtgg gaaaatcgtg aagagattct ggaaggtaag ttctacaaat ctaaatcatt
    34261 tacacctagt gaattggctg agttgaatta taatttagac cagtgtggct ttccaaaaga
    34321 ggaagaggaa atcttaaatc cctttgagtt gattcagaat tatcaagcgg aaagagcaac
    34381 tttaaatcat aagattgata atgtattagc tgatattttg cagttgttgg aggacaaata
    34441 atgacaccag aacaacttaa agcaagtatt ctccaaagag cgatggaagg gaaattagtg
    34501 ccgcaaaatc ccaatgacga acctgcaagt gaattattaa agagaattaa agctgaaaaa
    34561 gaaaaactta tcagtgaagg aaaaatcaaa cgagataaaa aggaaactga gatatttcgt
    34621 ggtgatgatg ggaaacatta tgggaagttt gctgatggaa gtactcaaga aattgatgtt
    34681 ccttatgata ttcctgatac ttgggagtgg gtgaggataa aatcaattta ttggaatttt
    34741 gggcaaaata agccagagaa atcctttagg tatatagata cgtctagtat tgatagaaaa
    34801 aagaacataa tcaactacaa aaatctacaa tatctttcac ctgaacaagc gccttcccgt
    34861 gctagaaaat tagtttcgca gaatagtgtc ttattttcaa cagttagacc atatctaaaa
    34921 aatattgctg tagttagaga acttaaagag tatttgatag ctagtacagc atttattgtt
    34981 ttggatactt tacttaacga aacatatttg aaatattatt tattatcaga taattttatc
    35041 aatcgtgtta ataataaatc tactggaaca agttatcctg caatcaatga ttataatttt
    35101 aatctattat taattgctct ccccccacta tccgaacaac aacgaata
//
LOCUS       DBEJAQ010000020        35013 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.020, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000020 DBEJAQ010000000
VERSION     DBEJAQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.020"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(381..1361)
                     /locus_tag="ACL297_001023"
     CDS             complement(381..1361)
                     /locus_tag="ACL297_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HGR0071302.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1678..3693)
                     /gene="recG"
                     /locus_tag="ACL297_001024"
     CDS             complement(1678..3693)
                     /gene="recG"
                     /locus_tag="ACL297_001024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGR0071303.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLI
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLALIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3712..4815)
                     /gene="alr"
                     /locus_tag="ACL297_001025"
     CDS             complement(3712..4815)
                     /gene="alr"
                     /locus_tag="ACL297_001025"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGR0071304.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4805..5167)
                     /gene="acpS"
                     /locus_tag="ACL297_001026"
     CDS             complement(4805..5167)
                     /gene="acpS"
                     /locus_tag="ACL297_001026"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGR0071305.1"
                     /translation="MIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLKGR
                     RQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLSIS
                     HTDQFVTASVILEENHES"
     gene            complement(5212..6243)
                     /locus_tag="ACL297_001027"
     CDS             complement(5212..6243)
                     /locus_tag="ACL297_001027"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGR0071306.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6245..7276)
                     /locus_tag="ACL297_001028"
     CDS             complement(6245..7276)
                     /locus_tag="ACL297_001028"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGR0071307.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7357..9870)
                     /gene="secA"
                     /locus_tag="ACL297_001029"
     CDS             complement(7357..9870)
                     /gene="secA"
                     /locus_tag="ACL297_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGR0071308.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10016..10303)
                     /locus_tag="ACL297_001030"
     CDS             complement(10016..10303)
                     /locus_tag="ACL297_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071309.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10331..10963)
                     /locus_tag="ACL297_001031"
     CDS             complement(10331..10963)
                     /locus_tag="ACL297_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0071310.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10967..11608)
                     /locus_tag="ACL297_001032"
     CDS             complement(10967..11608)
                     /locus_tag="ACL297_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071311.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11601..12392)
                     /locus_tag="ACL297_001033"
     CDS             complement(11601..12392)
                     /locus_tag="ACL297_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071312.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12414..12806)
                     /locus_tag="ACL297_001034"
     CDS             complement(12414..12806)
                     /locus_tag="ACL297_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071313.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            13133..13984
                     /locus_tag="ACL297_001035"
                     /note="rgg1518"
     CDS             13133..13984
                     /locus_tag="ACL297_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator
                     Rgg1518"
                     /protein_id="HGR0071314.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(14216..14566)
                     /locus_tag="ACL297_001036"
     CDS             complement(14216..14566)
                     /locus_tag="ACL297_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071315.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(14706..15041)
                     /locus_tag="ACL297_001037"
     CDS             complement(14706..15041)
                     /locus_tag="ACL297_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071316.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(15129..16439)
                     /gene="der"
                     /locus_tag="ACL297_001038"
     CDS             complement(15129..16439)
                     /gene="der"
                     /locus_tag="ACL297_001038"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGR0071317.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(16453..17166)
                     /locus_tag="ACL297_001039"
     CDS             complement(16453..17166)
                     /locus_tag="ACL297_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HGR0071318.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(17163..18059)
                     /gene="dnaI"
                     /locus_tag="ACL297_001040"
     CDS             complement(17163..18059)
                     /gene="dnaI"
                     /locus_tag="ACL297_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HGR0071319.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(18060..19229)
                     /locus_tag="ACL297_001041"
     CDS             complement(18060..19229)
                     /locus_tag="ACL297_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGR0071320.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(19230..19703)
                     /gene="nrdR"
                     /locus_tag="ACL297_001042"
     CDS             complement(19230..19703)
                     /gene="nrdR"
                     /locus_tag="ACL297_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGR0071321.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            19843..20208
                     /locus_tag="ACL297_001043"
     CDS             19843..20208
                     /locus_tag="ACL297_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGR0071322.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            20213..20908
                     /locus_tag="ACL297_001044"
     CDS             20213..20908
                     /locus_tag="ACL297_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071323.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            20925..21692
                     /locus_tag="ACL297_001045"
     CDS             20925..21692
                     /locus_tag="ACL297_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071324.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(22046..23245)
                     /locus_tag="ACL297_001046"
     CDS             complement(22046..23245)
                     /locus_tag="ACL297_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071325.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(23242..24135)
                     /locus_tag="ACL297_001047"
     CDS             complement(23242..24135)
                     /locus_tag="ACL297_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071326.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQEIYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            24258..24449
                     /locus_tag="ACL297_001048"
     CDS             24258..24449
                     /locus_tag="ACL297_001048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071327.1"
                     /translation="MKIKEQTRKLAVDCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYTYGKAKLTWFEEIFEEY"
     gene            24842..25030
                     /locus_tag="ACL297_001049"
                     /pseudo
     CDS             24842..25030
                     /locus_tag="ACL297_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558115.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            25445..26062
                     /locus_tag="ACL297_001050"
     CDS             25445..26062
                     /locus_tag="ACL297_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGR0071328.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(26382..27269)
                     /gene="scrK"
                     /locus_tag="ACL297_001051"
     CDS             complement(26382..27269)
                     /gene="scrK"
                     /locus_tag="ACL297_001051"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HGR0071329.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(27418..29301)
                     /locus_tag="ACL297_001052"
     CDS             complement(27418..29301)
                     /locus_tag="ACL297_001052"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HGR0071330.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            29678..31132
                     /locus_tag="ACL297_001053"
     CDS             29678..31132
                     /locus_tag="ACL297_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGR0071331.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRTSEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            31113..32078
                     /locus_tag="ACL297_001054"
     CDS             31113..32078
                     /locus_tag="ACL297_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGR0071332.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKPAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(32113..33387)
                     /locus_tag="ACL297_001055"
     CDS             complement(32113..33387)
                     /locus_tag="ACL297_001055"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HGR0071333.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(33387..34559)
                     /locus_tag="ACL297_001056"
     CDS             complement(33387..34559)
                     /locus_tag="ACL297_001056"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HGR0071334.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(34690..34938)
                     /locus_tag="ACL297_001057"
     CDS             complement(34690..34938)
                     /locus_tag="ACL297_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071335.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPISQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
BASE COUNT        10354 a         7757 c         6524 g        10378 t
ORIGIN      
        1 aacatatata cggcaaggcg aagctgacgt ggtttaaaga gatttttgaa aagtattaac
       61 acctacagac aagcctgacg atagcaagaa ctaccctact cgataggtat cggcttttgc
      121 tttctgaaaa aaattatttt aagcaatttg acaaatctag caacaaaaaa ttctataaat
      181 ataatagatt gaaactagaa tagtacacat ttgcttctaa aacattgtta gaaatcgatt
      241 tgactgtcct gatcgttttg tcctattctt gtttcatttt actatatttc catgataaaa
      301 cgcatagtat caagtttttt taatcccctg atactatgcg tgtttgtaat ttttaagatt
      361 ttgtgcttag agtcgactcc ttattttaga tatttaaaag gaatctcact tccacagagc
      421 cagttgtaga cttggtcatt gacaaataca ttcatggctt cgtgagcata ctcaggcatg
      481 atgcgatagg ttttatcgca ggtcagacga ttataaatcg caaactgggt aatgggatag
      541 caaacatcgt cgtccaagcc cgtaatcatc ttaacctcac cttggatacg atgggcaaga
      601 tttttgacat cgatataggc aagggtcgcc atgatttcct cctctgtttc atggaagggg
      661 tcgtgaaact tgaaataacg gaaaagttcg tcgtaagcct cgctagtatt accaatctca
      721 atcacccgtc tgaagtctga caagaaggga taaatggcaa ctgttttctg aattcgagga
      781 ttgagcgctg ctgcaactag agctagagcc cctccttgtg aggcaccata gctagaaaga
      841 cgcttctcat caacctgaga cagactagca acaatttcga ccaactggta aatatccaga
      901 taaacatcct tataaaagag gtggtcccga ccttccatag caccacggat aatatgcccc
      961 ttcacggtat ttcctaaagg agaacgcaag ccgtcttgtg agtaacctga ctggccccgc
     1021 acatccatgg aaacaacacc gtaaccagct acggtgaagc ccagcatgtc ggcccagtcc
     1081 cagccacgtc ccatataacc atgaaaatgg aagattaatg gaaccttctc ctcactcttt
     1141 ggaagaacaa tgcgtgcata gacctttcct tccttgcttc cttcaaatgt taactcatag
     1201 cacttgactt gaggaatgtg gaaatctctt tcctccaagt ggtaggatgg aagtgtggaa
     1261 acatttttca cttccccatc ccagaaagca tcaaagtctt ccggaacctc atcccttcct
     1321 ctataggtct taatttcttc tagcaaagct ggatttttca tagcatgctc cttttatttt
     1381 gtaatcgtta ccacaactgt agcatatcta tgtaatcaat gcaagaaaga agggtaaata
     1441 gaaagtgaat ttagattctt tacttttagg atgatgggct gaaagtagtt ttagaaacga
     1501 aaaaagagca tttcgctctc tcttctgttt atactagttt tttgcttcct tcttttggaa
     1561 agtggtttgg taatttactt taatcgttac tgtcatatga gagtcctcgt ttctttttga
     1621 tgactttagt ataagagcca aacctaaaaa ctagcttaag ttttccttag agaaagctta
     1681 atccagatgt tctttctttt ccagatgaag ggcaatcatg cgccactctg gatcttcttg
     1741 ccaagcttct atagaactaa tgtagctagc aacctttctt gcttcttcta aaatcggaaa
     1801 atcttcgata atatcagcca cttggaactc tggaagtcct gactgtctgg ttccaaaaat
     1861 ctcaccagaa ccacgcattt tcaaatcttc ctccgcaagg acaaatccat tggtcgtttc
     1921 tgtcatgata cgcatgcggt ctttcccaga atccgtcttg ggattagcaa cgagaacagc
     1981 gtaggactgc ttgtcccccc gaccgacacg acctctaagc tggtgaagtt gactgagacc
     2041 gaagcgatcg gcatccatga taatcatgac agtcgcattg ggaacgttga ccccaacctc
     2101 aataaccgtc gtcgaaacca gaatatccgt ctttctctcc ttgaaatcct gcatgatctg
     2161 gtctttttcg tcactcttca tcctaccatg tagaagagcc acctctgcct tgcctgcaaa
     2221 atgagtcgtc aactcctctg ataaggcaat ggcatttttc aaatctagag cttctgattc
     2281 ttcaatcaaa ggagagatga cataggcttg ggaacctttt tgaatttccc cctctaacca
     2341 agtcaagacc tgaggtagtt gctcatgttt gatccagcgc gtcacaatag gcttccgacc
     2401 tgctggcatc tggtcgataa tggaaacatc catatctcca aaggctgtga tggcaagcgt
     2461 ccgtggaatg ggagtcgccg tcatcatgag gacatctgga ttgtcacctt tttcccgtaa
     2521 aatacgcctt tgccctacac caaaacggtg ctgctcatcg ataataatca aagcaagacg
     2581 agcatactcc accccatctt gtatcagagc gtgagttccg ataatcaaat cagcctcacc
     2641 cttggcaatg gtctccaaga cctctctctt ttctgcagct ttcaaggaac ctatcaagag
     2701 agccagtttc aaattgggaa aaaggttctg taaactctca aagtgttgct ctgcgaggat
     2761 ttctgttggt accattaggg cagcctgata acctgctgtc actgccgcaa acatggccaa
     2821 gccagcgact accgtttttc cactccccac atccccttgt aggagacgat tcatgtggtg
     2881 gtcggacttc atatcagtta aaatttcctg caaactcttt tcctgagctt gggtcagggc
     2941 aaaaggaaga cttactttaa ctgctgtcac tttttcctga gaccaattca gaaccagacc
     3001 acttccctga actctatttt cagacttgag catctgcagc tgcatttgga aataaaagag
     3061 ttcctcaaac tttatacggc gaagagcctg cttgtattct gccaaatcct ttggaaaatg
     3121 catagctcgg actgcctgac aacgggacat gagtttgtat ttgtctagta aagactgggg
     3181 cagattttct tctatcaaga ggtccagtcc ctgatcaaaa gccgtcttga tgaccttgac
     3241 cagactggcc tgactgattc cctgagccag acgatagaca ggctggaggt catcttctac
     3301 ctgagccaga accttcatcc cagtcagact agccttagcg cggtcccatt ttccaaagac
     3361 agcaagggtt gctcccaact ctattttatc agccagatag ggctggttaa agaaattcac
     3421 cgcaaaaacg acctctccct gcttgagact aaaacgcagg cgattgcgct tgaaaccata
     3481 atactggaca ctagcaggag tcactacctg accagaaaga actgccttct caccgtcttc
     3541 cagctccagc acctgcttgg ttttgaagtc ttcataacgg aaaggaaagt agagcaagag
     3601 atcttgcaag ttttcaattc ctagtttggc gtatttttct gctgactttg gtcccacacc
     3661 aggcaagaca tgcaagggtt gatgtagatt catgctccac tcctttcttt tctaataata
     3721 ttctctcgga atacggtcgc tgaggaggca aaccacctca tagttaatgg ttacgcggta
     3781 ggtcgctacc tgagttgcag tgatttcctt atccccattg gagccaatca aggttacctt
     3841 ggttcctagc ggataaagct taggcaatcg aatagtgatt tggtccatcg aaaccctgcc
     3901 gacaattggg caagcttggc catctaccaa gacagagaaa ttttgcatgt ctcttgtcca
     3961 tccatctgca tacccgattg gcacggtcgc gatgacttgc tcgctatccg cttgataagt
     4021 tgctccatag cccatgcaag ctccagctgg aactgtcttg acatgaacca gagcagactc
     4081 caaggtcaag gccggtatca aatcataagg caaatccaag accgctccac ttggattgag
     4141 gccatacatg gcatctccca tacgaaccgc attgaaaata gtctctacat gccaaagagt
     4201 cgttgcagaa ttgctagcat gaaccagctc tggaacttcc ttcatactag ctaaaatagt
     4261 tttaaaccgt tctaactggg cattaaaata gtcatctgat tcctcatcag cagtagcaaa
     4321 gtgggtaaag attccttcaa cacaaacacc gtgttgttgg agcaaatctt gagcctgctc
     4381 aacctcactt gcctctctaa aaccaatccg tcccatccct gaatcaatct tgaggtggac
     4441 tgtcaatcca gttaggtcca cttccttatc taagagtgct tgaatccact ccagtccagc
     4501 cactgtcaag gtgaagtcat attctttagc tagagcaaca gcttcgattt cagaaactcc
     4561 taaaatgagg attggcttgc tgagtccagc ttgtctgagt tcaatggctt catcgatatt
     4621 ggaaacgcaa aagccatcaa catcatcttg aattgccttg gcaacggcaa cagctccatg
     4681 accataagca ttggccttaa ccacagccaa cttgagcgtt ccttgaggga tatgagcccc
     4741 catttgctga atattttgtc gaatagctcc cagatgaatc agagccttgg ttggtctatg
     4801 tggactagct ttcatgattt tcctccaaaa tgacactggc tgtcacaaac tgatcggtgt
     4861 ggctgataga cagccaaatc tttcctgaaa atggtgcctg actaaaataa ggcgccccac
     4921 gttcattgtt caagacttcc aaatcctgaa aaccgagctt gctaatgccc gttcccatag
     4981 ccttggaaaa ggcctcctta gccgaccagc gaccagctaa atattctatt tgcctgcgtc
     5041 ctttgagact ggtgaagcgc tccatttcct gagcggtcag tacacgctta gcaaatcctt
     5101 catgtcgtgt aactgcgctt tctatcgaag ccaattcttc gatgtcaatt ccgtgtccaa
     5161 ctatcattct catcaaaagg agttgagatt ccccaactcc atccttttca cttattttgt
     5221 caaggtaaca taaatctctt ctaccaaggc ctctgtattt tcccaaccta ggcaagggtc
     5281 agtaatagag caaccaaaga tctctggttg gttttgacga ccatctgcta ggtaagattc
     5341 aatcataaat cctcgaaccg tctttttaat tttctcattc caatcacgat tctgcaaggt
     5401 ctggcgaaca attcgaatct gctccatata ttgcttgcct gagttatcat ggttggtgtc
     5461 aatgaggata aaaggatttt caagtcccat ggtttcatag cgttcaatgg cttggagtag
     5521 attttcatag tagtaattcg gcatataatt gccatactcg ttgactgctc cacggaggat
     5581 aacatgggcc aaaggattac ctgatgtctc aacttcctgc ccatgataaa ggaaggtttg
     5641 cttgttttga gcagcataga tggcgttaaa cataacaccc aaatttcctg aggttggatt
     5701 tttcatccct actggtgcat caatcccaga agccacaaag cggtgctctt ggtcttccac
     5761 agaacgagct ccaacggcat ggtagctgac caagtcatcc accaagatca gatttgacgg
     5821 ataaagcatc tcatctgccg ttgtcaaacc agtctctgta atcacgcggt agtgcaactg
     5881 gcgcacagcc tgcaagccat taatcaggct tggagcctta gaagtatctg gctggtgaac
     5941 taatccttta tagccgtctc cattggtacg aggcttagca gtatacacgc gcatgaccat
     6001 gaaaatctta tccgctacct tcttttgcaa ggcagataaa cggcgagcat attccaagac
     6061 cgcctcttca ttatcagaag agcaaggacc aatcaccaag agaatacggt catcttcccc
     6121 tgaaataata tctgccaatt ccctgtcacg gctttccttg agtctcaagg cttccgcaga
     6181 caattgggtt tcagccttga tgacttccat atcgatttct tgaccttttt caataaatgc
     6241 catcttattc tcctaacgtt ttgtaaattt ctctgacaag agcttctgtg ttatcccaac
     6301 ccaggcaagg gtctgtgata gacttaccaa atacttctgg ctcattttgt cgaccatctt
     6361 ccagataaga ctcaatcata aaaccacgaa cgaactgctt aattttttca ttccaagcac
     6421 ggttaatcaa ggtctggcgg acaattcgga tctgttcaat atactgctta ccagaattgt
     6481 catgattggt atcaatgatg ataaaaggat tttccaagcc cattttctca tactgggcaa
     6541 tggtatcaat taaattgtca taatagtagt tgggaatatt ttttccatac tcattaagag
     6601 caccacgaag aatagcgtgt gaaagcgggt tcccagttgt ttctacttct tttcctaaga
     6661 aaaggaaact ttgtttgttt tgagcagcat aaatcccatt aaacatgaca ttgagatttc
     6721 cagaggttgg atttttaaaa ccagtagaaa atcctgcccc acttgccaca aagcggtgtt
     6781 gctggtcttc aactgaacgg gcaccaactg ccatgtaaga aatcaaatca tctacaagcg
     6841 gaaggttttc aggataaagc atttcatcag cagttgtcat ccctgtttct gtgatgacac
     6901 gatagtgaag atggcgaacg gctttgattc cattgataag actaggcgct tctgtcgcgt
     6961 taggctggtg aatcaagccc ttatagccat ctccgttggt acggggtttg gcagtataaa
     7021 cacgcataac cataaagata cgatctgcca cttcttcttg taggactgcc aaacgcttag
     7081 cgtattcaag gacagcttct tcgttgtcag atgagcatgg cccgattacc aagagaattc
     7141 gctggtcttc tccacgtata atggcttcta gctcttgatc tcgctgtgat ttcctctcca
     7201 aagcctgacc ttctaatttt gacaaggcac gaacttcttc tatatttatt ttagagcttt
     7261 ttgctgtaaa taccataact catctcctta taaagcaaac ggatacccag taaaaattta
     7321 cttttcacaa gatatccgcc tctaaactat ctcattttat tgtcttttac cgtgacagtt
     7381 tttaaatttc ttaccagaac cacatgggca aagttcattg cgtccaatct ggctcaaatc
     7441 caaatcttct ggcatacttg cttggtgagc agcgatattg cgagtcgctg ttgtactgat
     7501 atgacgttct gcctgtggtc tttcttgttc atgaatttgt gctttcatca tcaagcgtgt
     7561 cacatcaaac tcaatcgaac caatcatatc attaaacata cggaaacctt ctgcctgata
     7621 ctcaacaaca gggttgttct gagcatagcc acgaagtcca accgcgttac gcaattgatc
     7681 aagggcatcg atatgatctg tccacttgtt atccaccact cgtagaatca aaactttttg
     7741 gaattcttta actgcttctt catcgcgtag ttttgaaacc tgactatcgt aaaccttcaa
     7801 ggaacgttgg aaaagctctt ccttgatggc cttatcagac aagcctgaca agtcttccat
     7861 cgtaatagaa tcttcaggaa gcaagttgta cttagcaaag ttcaaaattg cctctagttt
     7921 ttcatcttgt ttggcacgcg catgaccatc aacgacacgt tcaatcgtgc gtttgatcat
     7981 agactgaatt tcaggtgcca agtcacgatc tgcagtgatg acatcgtaac gttgagcata
     8041 gataatctca cgttgttcac gcatgacatc atcgtattga aggacttgtt tacgggtatc
     8101 gtagttattt ccttcgacac gtttctgagc tgcttcaacc tgacgcgtca acatgcgaga
     8161 ctcaatggcc tcttcagaca tgttgagacg ttcaaagatt cccttcaagc gttcagaacc
     8221 aaaacgtttc atcaaatcat cttcaagaga taggtagaat tgtgactcac ctggatctcc
     8281 ttgacgacct gaacgtccac gaagctggtt atcgatacga cgactttcat gacgttctgt
     8341 accaataaca caaagtcctc caagttcacg aacaccttca ccaagcttga tgtcggtacc
     8401 acgacccgcc atgttggttg cgatggtaac ggcaccacgt tgaccagcat tcatgatgat
     8461 ttgggcttct ctatagtggt ttttggcatt caagacttcg tgaggaacac cagctgcaac
     8521 caatttctta gaaatgtagt cactagtttc aaccgctact gtaccaacca agacaggttg
     8581 acccttttgg taacgagcct taacgtcttc gacaaccgct ttaaacttag attcgatact
     8641 tgcataaaga aggtctgagt ggtcaatacg ttgaacagga cggtttgttg ggattggaat
     8701 aacacgaatg ttgtagattt cacgaaattc ttcttcctca gtcttacctg tacccgtcat
     8761 accagacaat tttttgtaca tacggaaaag gttttggtac gtgattgagg cagatgtctt
     8821 ggtttcatcc tggattggca caccttcttt ggcttcaata gcttggtgca atccatcaga
     8881 ataacgacga ccttccatgg tacgacctgt aaattggtcg acaatcaaga tttcttgctc
     8941 ttcgctcacc acatagtcaa tatcgagaag catgatgtag ttggcacgaa gggcgttatc
     9001 gataaagtgg gtcaaagcca cgttttcgat gtcatagagg ttttcaagtt tgaagtagct
     9061 ttcagccctg tcaatccctg aatcagacaa accaatagtc ttagactgca catcgatgat
     9121 gtagtcatct ttgttcaaag attttacata gtggtctgcc atgtgataca actgactggt
     9181 ttcaaccgca ttggcacctg atacaatcaa aggtgtacga gcctcgtcaa tcaagataga
     9241 gtcaacctca tcgaccaagg catagttaag cggacgttgt accatgtttt cagcgcgaac
     9301 gaccatgttg tcacgaaggt agtcaaatcc gatttctgag ttagttgagt aagtaatatc
     9361 acactcatag gcttcttttt tctccattgg agatttggta gccaagttaa tccctactga
     9421 caaaccaagc caagagtaca attcacccat ctcagtcgcg tcacgttctg acaggtattc
     9481 attaaccgta actacgtgaa cccctttacc tgaaagggca ttgaggtata ccggcatggt
     9541 cgcagttaag gttttccctt cccctgtacg catctctggc acgtcaccat ggtgaagaac
     9601 aatccccccc atgacctgaa ccttataagg gaagagacct aggacacgtt tggcaccttc
     9661 acggacaacc gcaaatgctt cgtaaagcaa tgaatccagt gattctccat tttgataacg
     9721 ttccttaaat tcaactgttt ttgcttttag ttggtcgtca gtcaaagcag ccatttggtc
     9781 ttcgtatttg aaaaccttgt cagccatctt ttccagacga cggatttctc ctttatcatt
     9841 ttcgataatt gtttttaaaa tattagccat gtttttcctt actttaaatt ccgaatattt
     9901 tagaatgttc ctttaatttt agcacaatta ctgattattt tcaaggaaga atccccattt
     9961 tgaaaaggaa aaagaggtta gttttcaagc taacctctcc gacttttatg actgtttaat
    10021 tgccaaaaat cggcaaaatg gcaaatgtga taaataagtt aatccaaaga gaaagacagc
    10081 aaatcagtcc aaaaacaaag agactgactt tcttggacag caaggccatg agaatggaca
    10141 gaattccaaa agctagcaag attttctgta tgatgaagat gtcaatcctt acccccagaa
    10201 tcgctctatc ccaagggata aagaagaaag acagccagat cagcagaact aaaccaatat
    10261 aaaccttgga atagcgtgta ataaatgatt ttagatgtgc cataagccat ctcctaggat
    10321 tgtatatcct ttacatttgt atatgtccat tttcgacaac taatacatct gtacaaaggt
    10381 ctgaaatatc ttccgatatg tgcgacgtca ggataaccag accattttct tttttcaggt
    10441 aagataaaat gacctgtttg gttaacctca cactcttctc atccaaagca ttcataggtt
    10501 catcgagaaa gagtatagaa ggagaggaaa taaaggcttg aatcaaggcc attttttgct
    10561 ttgttcccaa ggataaatga cggtattcaa cgtcttcaaa ttcctgtaaa tcatagtatt
    10621 gaatccaata ggcaattctt ttttccgtaa ctttagatga aaaatacctt aacagttcca
    10681 aattttctct cagggataaa tgagataaaa actcgacttt ttcaattaaa attcctgcat
    10741 cctgaatata atgatttttt atcccgtaga ctttcccgtc ttgaagaact tttcctttgt
    10801 caagcttaat atagccagca agtaccttta aaagaaccgt cttgccagat ccattatcac
    10861 ctttaagtcc ataaattttt cctgattgaa acaccaaatt caactgattt aaaataggtc
    10921 ttttcccata aattttctgt ccatttataa tttcaagtct catcgtttat cctttcttaa
    10981 atttctcttc caagcgacta acaatcaaca taactaataa acttaagagt agtaaataca
    11041 ctgttacaat atgatccatc atccattctt gtcccacaaa tactaaaaaa tagagaaaaa
    11101 aggaaaaacc agcgcccgca ctaatcagtg ctatcagata agacagtagt aagtacaaca
    11161 tcattaactg agcaagtaaa aagaaaagtg cgactgtttt gaaatgaatt cctagcggaa
    11221 gtaaaccaag gaaccagaca aacaaatctt gagtaaaaaa tttagaaatg acaaccaatc
    11281 gtttccttgt ataatcgaaa aaggagcttg attgatagcg aatcaagtga tggtaatggt
    11341 ctgccaaatc actttcaagt aaaatgacag agagccatcc taccatgaaa agcgaaaggt
    11401 ttaacaagag tttggggatt tctaaattcg cttcagaata gtcattatag gaaaaaattg
    11461 aaatcaattc tctcttgaaa aagataacag catcctgctt ttgaaccgct gttgacaata
    11521 gatggtttat caaaatcatc atcagaacta tacgaagtag taaaacaatc tttctcttta
    11581 gcaaaatatt gttaaaattc ttaaacatta tcttcatatt tttcaagcac catgtaaggg
    11641 acaatataaa ggatataagg agtaaagagt ttcatcaaac ttacatcccc atagctagct
    11701 tgcatcaaac tcgtcatagg aaccatatag taaggcaagg cactatagag cagcatagaa
    11761 ccaagccaaa gaaacatcaa ataggtgatt gccgaacgag tcacattccc cacataatca
    11821 actatttgta aaaaatagat tgcattgatg aaaataccga ttagcaggac acaagtaaag
    11881 aacaaatagc tttttatctc tccatctagg agtctttgta aaccacttcc atcagaaaat
    11941 agagaattcc atccaagagg agaaaaagtc ccaaggaaat aggttataat tgcaattgtc
    12001 agcacagtca ctaaatatat caaacaaggg atacttgcaa cttgcaaagt caactttctt
    12061 tttaaccctg gatagctcac ttctcttcca atactcaatc gtaaaacttt atttttcagc
    12121 tcaatatatt gaaaacctag caaaacaatg atgattggaa taataaatga ttgataaaaa
    12181 cctagattga actggagaat acgaaaatct aatgctgatt ttaataagtc atttttaatt
    12241 gcttgtccat cgaatttagc tagatcgggc gcatgaatat caaaaaagaa ataagtcatc
    12301 aaaacgtcac gataaaccaa agcaagaaaa agcaaagtaa gcaagacata aacaaattta
    12361 tgccttgcca aagttaagag aggaatcttc ataaattata cctactttac gaattaattt
    12421 ggtgaccaac tacctgaaat ggcagctgat aaatcccacc aattttgtct tgccatatct
    12481 gctgcataaa catagccaac cgttgcccaa ttaccatgag ttgaacgagt accacaggtt
    12541 gttaacgatt ctccactacg agcttctcca ttagaattta ctatacgatg cttaacagtc
    12601 atgggatgta aatttctaca aggatttaag ttcatgatat attccgcacc ttcattttct
    12661 ttagtcaaat aacctgttct cttataactg gtagtaataa caaaattact aaaagcacct
    12721 atctgatttg cttctacgaa agtagttcca acagcactta tcattaaaac aggaataacc
    12781 attttcaata atttcttaat tttcattttt tctgtttcct ttcatttttt attcaagaag
    12841 attccttata acttctattt taaatataga cttattctaa acgatggtat atcatacaaa
    12901 ttcctccttt acttattata tatagactct aatttcttta tccccaaaac catgtttcaa
    12961 accagataag ttgaatccaa ctccaaatca aaaatccaga gtttttcggt aaattcttca
    13021 aatatttttt aaagcccatg actttttccc tcctttttga tataataata ccaccttttt
    13081 ctacgttttt tataaatgta gaaaatattc acatttacct aggaggaaca atatgagatg
    13141 ggattatgga caaattttta aagaaattcg aaagtcaaaa ggattgaccc aacaagatgt
    13201 atgtggacaa gtcatacatc gaacaactct aacaaatatt gaacacggta aagttattcc
    13261 cagttttgaa aacatggtat ttcttcttga acaaattgat atgagcttgg cagaattcaa
    13321 gtatatatgc aacgaatacc atcctagtaa aaggcgagat attattgtag agagccaaaa
    13381 tccgtctact tttcaagata ctagaaaaat ggttgaactc actgagaaat gtcaaaaata
    13441 tcttaagaca catcacgatg ttcctattca aaatatctat cgtcatacaa aaattgtcac
    13501 agagttacga actaaaggat tcaaaaacaa ccacgtcttg aaagatttgt atgaagaaat
    13561 ttgggactat cttgaaccta tggatacatg gtacattagt gatttgaaat tgcttggaac
    13621 cattctcttt ttctttcctt ctgaaaatct tccccttctt attgatagaa ttatgaaaac
    13681 catcgagaaa tataaatact tccgagaaac aaaagcattt ttatcatctt ttttagccaa
    13741 tctctccact gtatattttc aacatcattt attcaaagaa tgtgaaacaa tcaccctaca
    13801 acttttggta ttagcagaag aattaaaaat atacgatatt cttggattct ctcaagtacg
    13861 cttgggaata ctacaacata actccgattt gattgataaa ggaataactt tattacgact
    13921 aaccaaagaa gaggcattag ttaaaatact cgaaaaagaa attaatgatt tttcaaacct
    13981 ataatttgta ataactctca tcattttatt atagtagatt gtaatagaat gtaatagaat
    14041 gtgaacgaat caattagggg atctaaagca ttttatacca atgttttata agttgtattg
    14101 tattattcca tattcaatcc actatacaag atgaatattt ctctcaagtt tcataatcaa
    14161 aataaaaatg actgtatcta tagctccata aacaaatcat agatacagtc attttttagt
    14221 tcctacacct tcctttatgc acacctttag atttccctag ttcccgcctc aagcgaatca
    14281 tttttttgaa aaagacatag gctaaccaat cactagcaaa agcgataagt agtaataata
    14341 gccaaatacc ccataacttg atatctgtca aatttctcaa gacgatattg aaaaacagaa
    14401 ctgaaaaaac tgtccaagca aggctaaaaa gagcatagag ggtgatgtaa atccagtaaa
    14461 aatagtaaaa aattgggaaa aatttacttt ctctattatc cttgtcaatc cagctgaaaa
    14521 agtaaaaaca gggtaataag attatcattt taaacaagtg ttccatcaca agcctccttt
    14581 tttaaattgt tttctttagt ttcgttctat taaatgttat taccaatata aaccttggaa
    14641 tagcgtgtaa taaatgattt tagaggtacc ataagccacc tcctacaaat agaaaccgat
    14701 ataaatcaat gccttccacc cttagacttc cctagttcct gtctcaagcg aaacatttct
    14761 ttgaaacagg aataagttaa ccaattcata ccaatagcta gcagaataaa aagaaaccaa
    14821 atgccccata acttgatatc tgtcacattt ctcaagacgg tattgaaaaa cagaactgaa
    14881 acaactgtcc aagcaaggct aaaaagagaa tagaagggga tgtaaaacca gtaaaaataa
    14941 taaaaaattg gaaaaaactt actatttctg ttggcctttt caatccagtt atcaaaataa
    15001 aagtacggtg ctaaaagtaa gaatttaaac aaatgttcca tcaccgacat cccccttctt
    15061 ttgatagcgt tttctattat tttattatat caaaaaaatc cggaactgtc attccagatt
    15121 ctactttttt atttgcgttt tcttgcgatg agatgaatcg gtgttccctc aaaaacaaag
    15181 gccttgcgga tttgattttc caagaaacgc aggtaagaaa agtgcatgag ttcttcttca
    15241 ttgacaaaga tgacaaaggt tggtggtttg gttgccactt gggtcgcata gaaaatcttg
    15301 agacgttttc ctttgtctgt cggtgttggg ttgatggcaa tggcatccat gatgacatcg
    15361 ttcaagacag ctgatggaat acgtgtattt tgactttcgc tgatttgctt aatcatctca
    15421 ggaagtttgt ggagacgttg cttggttaaa gctgatacaa agataatcgg tgcgtaaggc
    15481 aggtattgga actgctcacg gatatcttct tcccagtttt tcatagtgtg gttatctttt
    15541 tcaagcgtat cccacttgtt gaccacgata atcatccctt taccagcttc atgagcaaat
    15601 ccagcgatac gcttgtcgta ttcacgaatc ccctcttccg cattgatgac cattaagacc
    15661 acatctgaac ggtcaatagc acgcatggca cgcatgacag agtacttctc agtattttca
    15721 taaaccttac cagacttacg cataccagcc gtatcaatca tggtaaactc ttgaccatct
    15781 gtatctgtaa agtgggtatc aatagcgtca cgagttgttc cagcaacagg actggcaatg
    15841 acacggtctt ctcccaagat agcattaatc aagcttgatt ttccaacgtt aggacgacca
    15901 atcaagctaa acttaatgac atctggattt tcttcttcat attcatttgg aaggttttcc
    15961 acaatcgcat ctagtacatc ccctgtaccg ataccatgga cagatgagat aggcagtggt
    16021 tcacccaaac cgagagcata gaaatcatag atatcatttc tcatctcagg gttgtccacc
    16081 ttgttgactg cgaggataac tggtttgtgg gtcttataaa gcttacgagc tacgtattcg
    16141 tctgcatcag taattccttc cttaccagac acgacaaaaa cgataacatc tgcttcttcc
    16201 atggcaattt ctgcctggtg cttgatttgt tccatgaaag gagcatcgac atcatcaatt
    16261 cctcctgtat caatcatgct aaaagaacga ttgagccact cacccgttgc ataaatacgg
    16321 tcacgtgtca ctccttcgac atcttctaca atggagattc gctcaccagc gatccgatta
    16381 aatagggttg atttcccaac attgggacgt cctacaatgg caatagttgg tagggccata
    16441 atttctcact ttctacaata atttcttctg ttcaagattt tttctagttg agcttggttc
    16501 agcttgacca aactgttctg ctaggcgctg actccagctt gtggtcgcac gcgccccagc
    16561 atagtcagcc tgaacacggt cataagcttg gattgcctca gttgactgtt cttggtattc
    16621 ttcctcaaag acaacattct ctagtggcag tctcggtttc atatcatgat gttgatttgg
    16681 cacacccagt gccatcccaa agacagaata ggtgtagtca ggtaggttaa agagctctgc
    16741 cacttcttca gacttgtatc gaaccaaacc gataatcaca ccaccatagc ccaagctttc
    16801 agctgccaac aaggcgtttt gtccagcaag agctgcatcg accgaactaa tcaagagacc
    16861 ttccacacct tggggttgga aggtgtcggt atgaagtctg gctccctttt ctgctcggtt
    16921 caaatctccg acaaagagaa ggaaaacagc agactggcga atggcttctt gaggtaccaa
    16981 ttcatacaag gcatctttct tctcttgact tcgtaccaca atcacagagt aggattggaa
    17041 attcttccaa gatgatgcca tctgggctgc tgtcaaaatc tcatttaagt ctacttgggg
    17101 aatttcttgc tctttaaacc tgcgcactga agtatgagcc ttcatcaatt taatggtttc
    17161 tgtcatcgac ggtttactcc ttctaaacga gtctcctcag ccaaataacg gatgcgttcc
    17221 atgacccgtc tggcttccca ggtttcgtca tttccatgtt tcactttcgc aaaatgcttc
    17281 tccaaatctt caaagttgaa gttggatgtg aaaaaggtcg gtaaattttc ctgcatccga
    17341 tattggagaa tgacctgcag gatttcatca cgcacccaag cggtggattg ctcggcgcca
    17401 atatcatcta aaatcaggac ctcagacagc ttaatctcat ccaccaaggt cttaacattg
    17461 ccatcactga tagcattttt gacatcaatg acaaagctag gatagtggag gagagttgat
    17521 gaaacaccac gtttttctga taaatcatga gctaaggccg ccaccatgaa acttttaccc
    17581 acaccaaagt ctccatataa gtaaagacct tttcgaatag ctggatattg ctccacgaag
    17641 gctagtagct tttcaaaaac tggtaagcgc cccaaatcat ccaagtcaac ttgagccaaa
    17701 ctagctttct tgagactggc tggtagattg attaacttga gacggttctt aatagccgct
    17761 tctttttcag ccgcgattag ctcaggagtt tcttcatatg aaacatctgc atagccatga
    17821 ttcttaacca aaatcggctt gtagcctttg gcaatataat ccgtatcccc acggagaaac
    17881 ttgtcacgct cggtgatgta ctgattaaac ttggagatac tgcgatttaa ttcctctgga
    17941 gtcaaggatt cttgctggat aaaagccgca acatcagggt ccttcatgat tttctggacc
    18001 aaatcttgat aataaaaacg gctgggttga cgtttgagta cgtctccgac actttccatc
    18061 taatctcctc ctttttctaa tcgagctaat agttcttgct tcttacgttc tagttccaga
    18121 cgagtttcct cgctggtttc attcttatat tcaggattac tccatttagg aacattggtt
    18181 tttactgggg cagtctgatt ctgtttttgt gtttttgctt tctgtcctcg ctcacggatg
    18241 cgcaagactg ccttttctgc cgaatgaatc ttttgatagg catagtcatt ggctaccttc
    18301 atggcatatt tctcattgat atttgccgaa tcgaccttat taaaggtcaa taaaagaatg
    18361 atattgatga cttcgtccag caagcccaag ccagccatct gttgcaagag ttctctttct
    18421 gtttgggtaa tggttccctt gcgtgtttgc ttgatttctg ccaagaactg cagggcagtt
    18481 ttacttttgg cttctttgat aatggttgtt tccttaagac taaagtcaga ggaaactggt
    18541 ttttgagcaa ttttttcacg catgcgtttg gttgaaataa cctgagaaac agctgttgac
    18601 ttggccaatt gataggtttc aaaccaagtc catttcttct cctcggcaat agcaaagagg
    18661 tttaagacat cggactgctc atccgcaaaa cgaagtccat ctcgagccat cagctggcga
    18721 aaatgttcca agtcaaaatc attggccact ttcttcttga gaccaaggtc ttcttgactg
    18781 cctagttctg ccaattctgg aaagacttga ttgagtgaga caggtatttc ttcaccatca
    18841 gcactttcaa ctttcaaatc ctccacagca gagtcgccaa tctttttctc taagagtctg
    18901 cgataaacag gatgctccaa gaagtcttga ctagatagag gagcatggag ggctagctga
    18961 taaacatccc ccttttgata gagggtcaag agattaaaag cagataagat tttcaatgat
    19021 tttatcagtc tatccatccc aaagttgaga tgattgagga tacttgaaaa aagatattcc
    19081 tttctaccat tatcccaaaa actgattgta taaagataaa ggctcagtgc ctcctgaccg
    19141 ataatcggga ggtagcactg taccagagat gaggtatctt gcgacacccg attattcttt
    19201 agataagaaa aacggtcaat tggcttcatt tatctttcct ttttcttttt agaggactgg
    19261 gtgatttgtt ggagcaagct ctctaactca ctgacatcct taaaactacg atagacacta
    19321 gcaaaacgta cataggtaat ctcgtccaat tcagccaact cctccatgac gagtgaacca
    19381 atgtcctcac tttgaatttc attttcattt cgaccacgga gtttctgttc gatacgattg
    19441 actaccatgt tgatttcatc acttgacaca ggacgtttct gggctgagcg gataatccca
    19501 ttaaagattt tatctctgga gaattgttcc cgtgtgccat cttttttaac aaccactaag
    19561 gttctttctt ctactcgttc gtaggttgta aaacggtgtt ggcactcgtc gcactctcgt
    19621 cttctacgaa tggtgttccc ttcttctgct tggcgactat cgataacact tgacttggta
    19681 gccccacatt ttggacaacg catcctttcc ctccttatcg ttttcttttc attataccat
    19741 tttttaagcg attcccaaaa caattcttct ttttgcttga caagtttttt gttttgttgt
    19801 attatttaat taagacaaaa ggataagaga aaggagacta agatgtcctg gacatttgac
    19861 aacaaaaaac ccatctattt acagattatg gagaaaatca agcttcagat tgtttcccat
    19921 acactggaac ccaatcaaca acttccaacc gtgagggagc tagctagcga ggctggtgtc
    19981 aatcccaata ccatccaaag agccttatca gaccttgaac gagaaggatt tgtctacagc
    20041 aagcgaacaa ctggacgatt tgtgactaag gataaggagc taatcgccca gtcacgcaaa
    20101 caattatcag aagaagaatt ggaacacttc gtttcctcca tgacccattt tggctatgaa
    20161 aaagaagaac taccaggcgt agtcagtgat tatattaaag gagtttaagc ctatgtcatt
    20221 actagtattt gaaaatgtat ccaaatcata tggagcaaca ccagcccttg aaaatgtttc
    20281 tcttgacatt ccagctggaa aaattgtcgg ccttcttggg ccaaacggct caggaaaaac
    20341 aaccctgatt aaactaatta atggcctctt acaaccagat caaggacgtg tcctcatcaa
    20401 cgacatggac ccaagcccag caaccaaggc cgttgtagct tatttgcctg atacgaccta
    20461 tctcaatgag caaatgaagg tcaaagaagc cctaacctac ttcaagacct tctataaaga
    20521 tttcaatctt gaacgcgccc atcatctact tgcagacctg ggcattgatg aaaatagtcg
    20581 tctcaagaaa ctatcaaaag gaaacaaaga aaaggttcaa ctgattttgg ttatgagccg
    20641 tgatgctcgt ctctatgttt tggacgaacc cattggtggg gtggatccag cagcccgtgc
    20701 ttatatcctc aataccatta tcaacaacta ctcaccaact tctaccgttt tgatttctac
    20761 ccacttgatt tctgatatcg agccaatctt ggatgaaatt gtcttcctaa aagacggaaa
    20821 agtcgtccgt caaggaaatg tagatgatat tcgctacgag tcaggtgaat ccattgacca
    20881 actcttccgt caggaattta aggcctaagc aaaggagatt atttatgttt tggaatttag
    20941 ttcgctacga atttaaaaat gttaacaagt ggtatttagc cctctacgca gccgtgctag
    21001 tcctttctgc cctcatcgga atacagacac aaggctttaa aaatctacct taccaagaaa
    21061 gtcaggctac tatgctactt tttctagcta cagtctttgg tggcttgatg cttacacttg
    21121 ggatttcaac cattttcttg attattaaac gcttcaaagg tagtgtctac gaccgacaag
    21181 gctatctgac tttgaccttg ccagtttctg aacaccatat catcacagcc aaactaatcg
    21241 gtgcctttat ctggtcattg attagcaccg ctgtattggc tctaagtgct gttattattc
    21301 tggctttaac agctccagaa tggattcctc tttcttatgt gattacattt gtagaaacac
    21361 atctccctca gatctttctt acaggtatat ccttcctact aaatactatt tcaggaatcc
    21421 tctgcatcta cctggctatt tccattggac agcttttcaa tgaataccgt acagcactcg
    21481 ctgttgcagt ctacattggt atccaaatcg tcattggatt tattgaactt ttcttcaatc
    21541 ttagttctaa tttctatgtc aattcactgg taggactcaa tgaccatttc tatatgggag
    21601 caggtatagc cattgttgaa gaactcatat tcatagctat cttttatctc ggaacctact
    21661 acatcttgag aaataaggtt aatttgcttt aaataatttt tacctagata tgtaacatac
    21721 tcatagaaca aaagagacca ggcaaaaagt ctttacaatt agaaaacgca tagtatcaag
    21781 tgttaaaaat ccttgataat tatgcgtttt ttgtgagctg acttatttcc tttcactata
    21841 tcgcaaaatg aaataagaac ggaacgatgg gattttggaa ttcaaatcaa tttataagaa
    21901 tgttttagaa gtaatattat cctattccag attcagttca ctatacaatt gagttttcaa
    21961 gcaacctgtt tacataatgt gtacataatt aggttcgtga ttccaccctt ttcaccttta
    22021 aaaacctcgc tttcgcaagg ctcttctatt tataagataa ggcacgttta aaggttttcc
    22081 aaatccctaa atcatccgtt tgaagaacga gactagcata catgcgtccg ataaatcctg
    22141 ttgctaccac cgcaaaaatc actgtaatag caagtgaaat ccatgcttcc gctccctccg
    22201 catagccatt aatcgttcga aacggcataa agaaggtcga aataaaggga atataagaac
    22261 caatcttcaa gaggagattg tcaccagctg cacctagagc tgtcactcca aaaaaaccac
    22321 ccataatcaa aatcatcaaa ggcgacaagg ctttccctga gtcttcagga cgagaaacca
    22381 tagatcctag gaaggctgcc aagactacgt acatgaaaag actgatcaaa ataaagagca
    22441 aggtattcag tgagatagca tctcccaagt gatccaaaat accagactga gccaagaatg
    22501 gcaaatcttt aaagagcaaa acggcagcca gaccacctac aacatagatc ccaatatgcg
    22561 ttaaaatcac tagaaacaga gccatcatcc gcgcatagaa atagtgactt gcccttatgc
    22621 tagaaaagac gacttccata attttggtgc ctttttcact ggcaacttcc tgagctgtta
    22681 cacccgcata ggtaatcaga atcatataaa gaaagaatcc taaggcacct gctgcaattg
    22741 tttgaataaa ctttttattt tccttggctt catcaatctt ttctgtgaat tgaattgtct
    22801 gcgctaagcg tttttcctgc tcttgagaca aggaagcagt tgaacgatta agctgatttt
    22861 gcagttcatt gagtgtacct gtaacctcaa atttaattcc attttcaagc gatgtttcgc
    22921 catgataaac tgcctttaga acactatctt cttgatcaat ggtcaaataa ccttttaatt
    22981 tttcttcttt aattgcttct ttggcacttg cttcgtcttt atagtcgaag ttaacaccat
    23041 ttacattctt cagtccttct gctacagatg gcactgttgt cactactgcc actttattat
    23101 ttttagccat agaagaacct tggagatgcc caattcctac agagattcct aaaaagagga
    23161 acggcgaaat caccataaag aagaaactcc atgactcgac atgtcgaaga taggtttcct
    23221 tgattacaac ccacatattt ctcatacttc cactcctgat tctagtttaa agatttcatc
    23281 gatagttggc gcttgttggt caaatgttgc gatatattga ccttgagtca agattgggaa
    23341 gagttccctt ccagcgctct catcctccaa aatcaatttc caactgcctt gtttggtcaa
    23401 gctcacctgt ttgacatgag gaagattttc caattcttcc ttgcttcgtt cacttgaaac
    23461 aaagagacgc gttttcccgt attgattgcg gacatcctga actggtccgt gcaagaccac
    23521 acggccatct cgaatcatca gaatatcgtc acaaagttcc tcaacattgg tcatgacatg
    23581 gtcagaaaag ataatggttg ctccgcgctc tttttcctga aaaatgactt gtttgagcaa
    23641 ttctgtatta actgggtcca atccactaaa aggctcatcc aagataatca ggtctggttc
    23701 atgaatcaga gtaataatga gctgaatctt ctgctgattt ccttttgaca gactcttgat
    23761 tttatctgtc agctttcctt ttacttccaa cctcttcatc cattgaggga gtttttcttt
    23821 gacttctttg gcatccatgc cttttagagt cgccaagtag cgaacttgtt caagaactgt
    23881 caatttaggc atgagactgc gttcttcagg cagataacca atccgagcat agatctcctg
    23941 acgaatatcc tgaccatcca gaccgatttc tccctgatat tctaggaatt ttaaaatact
    24001 atggaaaatc gttgtttttc cagcaccatt ttttccgact agtcccaaaa tacgacctgg
    24061 tcgcgcttga aagtcaatac caaacaaaac ttgcttggat ccaaaacttt tctctagact
    24121 tcttacttct agcatctttc acctccgaaa tttcttgcac tcattatact cctttttgat
    24181 agcctttaca atgttttttg tccattttta gaagactatt gctgtgtaaa atatggcctg
    24241 gagcactttt atactcaatg aaaatcaaag agcaaactag gaagctagcc gtagactgct
    24301 caaagtacag ctttgaggtt gcagataaaa ctgacgaagt cagctcaaaa cactgttttg
    24361 aggttgtgga tagaactgac gaagtcagta actatacgta cggcaaggcg aagctgacgt
    24421 ggtttgaaga gattttcgaa gagtattagt gataaatcca ttatacagca gcaaacttaa
    24481 tttatacctt ccgctcctca actgtctatt tttaatcctg aattgttatt tgagtaactc
    24541 ctttttcctc gtaaagtttt cttcctctaa aacttctgga aaaaggctaa tagtttcaga
    24601 caacattttt ataagaaaca agttcatctg tcatttcaag aaggagtaat cctttatcta
    24661 ctaatggacg gaacagaatt caaccgcttg tccgatatgt tttctaagga ttatatagta
    24721 aaatgaaata agaacaggac aaattgatca ggacagtcaa attgatttct aacaatgttt
    24781 tagaagtaga tgtatactat tctagtttca atctgctata tctattatgc acacccctat
    24841 aggatctaat gaaaatcaca acaggctcat tcatagatag ttacctcagg aaactaagaa
    24901 aacgactaca aaggaagtcg cattcatcga aaagtggatt aacaactatc ctaaaaaatg
    24961 cttgaactac aagtccccca gagaagactt ctggatgact aacttgaact tgaaatttag
    25021 caataattaa ttcaccatct aactatattt agtaattatt tcagaactga ttaatattaa
    25081 aattaactaa caattcaaag gattcatact agccataaat tacgtccatc agagagagac
    25141 tcttactact tttagatttt agtctttcta gcttcagaat acatctaaac tttagggaaa
    25201 atgactattc gaaagcgcga atgcctcaaa attatctcag ataagctatt cgaaacttag
    25261 aatgctttta aatttatgga attgcgatta ttcgaaacct agaatgcata taacctttag
    25321 ttgacagacc tattctaagt ctcgaagggc tatttacttt ctattcctta tcaaaaaaga
    25381 ctcattcccc ctttctcctc caaaatatgg tatagtagaa atatactatc tatgaggagt
    25441 ttacatgtca caggataaac aaatgaaagc tgtttctccc cttttgcagc gagttatcaa
    25501 tatctcatcg attgtcggtg gggttgggag tttgattttc tgtatttggg cttatcaggc
    25561 tgggatttta caatccaagg aaaccctctc tgcctttatc cagcaggcag gcatctgggg
    25621 tccacctctc tttatctttt tacagatttt acagactgtc gtccctatca ttccaggggc
    25681 cttgacctcg gtggctgggg tctttatcta cgggcacatc atcgggacta tctacaacta
    25741 tatcggcatc gtgattggct gtgccattat cttttatcta gtgcgcctat acggagctgc
    25801 ctttgtccag tctgtcgtca gcaagcgcac ctacgacaag tacatcggct ggctagataa
    25861 gggcaatcgt tttgaccgct tctttatttt tatgatgatt tggcccatta gcccagctga
    25921 ctttctctgt atgctggctg ccctgaccaa gatgagtttc aagcgctaca tgaccatcat
    25981 cattctgacc aaacccttta ccctcgtggt ttatacctac ggtctgacct atattattga
    26041 ctttttctgg caaatgcttt gacacgtaaa aaatccgttt ggtttcccaa gtggattttt
    26101 aaagcgtaga ttaactatag cttgatacta aatatacttt ggtatggaaa tcatgcatat
    26161 ttttcgatag tgaagcgagg acttacctag cctttccacc gtgatagaaa cacctgaaat
    26221 ctaatggttt caggtgttcg gaaactttga gcctagtgtc tcaaagttta ggtatggaat
    26281 ttcgaagaaa gtcgctaccg tccgtaatca cttaaggaaa ggctcaaaaa tattgttttc
    26341 aaccacaaaa tccgtttggt ttcccaagcg gattttgtgc tttattttga aacttctttt
    26401 gcaagaacaa agttcccaag tgtggcagaa ccatttcctg cgactgctgg cgtcacgata
    26461 tagtcacgca catctggtac tggtaggtaa ccattaagaa gagatgtaaa tttctcacgg
    26521 acacggtcca gcatatgttg ttgagccatg acccctccac caaagacaat cacgtctggg
    26581 cggaaagtca ctgtcgcatt aaccgcagct tgagcgatat agtaggcttg aacatcccaa
    26641 acagggttgt tgagttcaat agtttcccca cgtacacctg tacgagcttc caaacttgga
    26701 ccagctgcat agccttccag acatccctta tggaaaggac aaacaccctt aaactctttt
    26761 tcaatatcca ttgggtgtct agcaacataa taatgaccca tttcagggtg acccacacca
    26821 ccgataaact caccacgttg gatgacgcct gcaccgatac ctgtaccgat tgtgtagtaa
    26881 accaagtttt cgatacgacc accagcattg ttacgggcaa ccacttcacc gtaagcagag
    26941 ctgtttacgt ctgttgtgaa gtacattggc acgtttaggg cgcgacgaag ggcaccaagc
    27001 aagtccacat ttgcccagtt tggttttgga gtcgtcgtga taaagccata agtttttgag
    27061 tttttgtcaa tatcaatcgg cccaaatgaa ccaactgcaa gaccagcaag gttatcgaat
    27121 tttgagaaga actcaatggt tttatcgatt gtttcgattg gagttgttgt tggaaattgt
    27181 gttttttcta caacgttaaa gttttcatca ccgacagcac agacaaactt tgtaccgcca
    27241 gcttccaagc ttccatataa ttttgtcatg ataaacctct tgtttttatt ttctttatta
    27301 tagcatactt cgaaagtcta aatgtctcta ttttttagat tttcctctgt aaatcttact
    27361 atctaataaa aacgaacaaa catgtcattt gttcgttttc acattagaga ggattgatta
    27421 gattttcact tcgatcacag catccccctt cgcaactgaa cctgttgcga ctggagctac
    27481 tgaagcgtag tcagctgtat ttgtaacgat aaccattgtt gtatcatcaa gtccagctgt
    27541 agcgatttta tttgagtcaa atgttccaag aacatcgcca actttcacct tatcaccttg
    27601 agcaactttt gcttcaaaac cgtcaccgtt catagataca gtgtcaatac caacatgaat
    27661 caaaacttca gcaccatttc ttgttttcaa accaaaagcg tgccctgttg gaaaggcaat
    27721 tgaaacttca gcatcagctg gtgcatagac cacgccttgg cttggtttca cagcgatacc
    27781 ttgtcccata gctccacttg agaagactgg gtcattgaca tcagcaagag cgacaacatc
    27841 accgacgata ggagttacaa gtgtttcatt ttgaagagct gctggcgcaa cttcttcttt
    27901 ttcttcagcc acttcagctt gttttgcagc tgcagttgcg tctacttcat cttcgtaacc
    27961 aaacatgtaa gtaagagcaa aaccaagggc aaatgataca gctaccataa gaaggtattg
    28021 tggaagttgt ccgttaccaa cataaagcat tgtaccaggg atgatggtga taccattacc
    28081 agtaccagca agtccaagga tagaagccaa tccaccaccg attgcaccag caatcaatga
    28141 aaggaagaat ggtttacgga agcgcaagtt caccccgaag atagcaggct ctgtaatacc
    28201 taggaaggca gaaagagcag ccgggaaagc aagtgttttc agttttggat tttttgtttt
    28261 aacaccaacc gcaacagtag cagcaccttg agctgtcata gcagctgtga tgatagcgtt
    28321 gaatgggtta gcatggtcag cagcaagtaa ttgcacttca agcaagttga agatgtggtg
    28381 cacacctgac acgacgatca attggtgaac cccaccaatc aagaaaccac caagaccaaa
    28441 tggcatgcta agaatcgctt ttgtagcaat aaggatgtag ttttcaacaa cgtggaaaac
    28501 tggtccaatg acaaagagtc caaggataga catgaccaaa agtgtcacga atggtgttac
    28561 caagaggtca atgacatctg gaacaacctt gcggacagct ttttcaaatt tagctccgac
    28621 aaccccgatg atgaaggctg gaagaacgga accttgcaaa ccaacaacag ggatgaaacc
    28681 aaagaagttc atcgctgtta cttcaccacc ttgagcaact gcccaagcgt ttggaagtga
    28741 gccagagaca agcatcatac caagaacgat accaacggca ggatttccac caaatacacg
    28801 gaaggttgac cacacaacca aacctggcaa gatgatgaag gctgtatctg tcaagatttg
    28861 tgtgtaagtt gcaaagtcac ctggaagtgg catttcaaga gcgttgaaaa gaccacgcac
    28921 acccatgaag agacctgtcg ctacgataac tgggatgatt ggaacgaaaa catcaccaaa
    28981 agtacggata gcacgttgga accagttccc ttgtttagca acttctgctt tcatgtcatc
    29041 cttagatgat gttggtaatc caagtacaac aacttcatcg tacattttgt taactgtacc
    29101 tgtaccaaag ataatttggt attgccctga gttaaagaaa gcaccttgaa ctttttccaa
    29161 gttctcaatc acttctttat tgattttctc ttcatctttg accatgacac gtagacgtgt
    29221 cgcacagtgg gcaacactat tgacattttc acgtccgccc aaggcatcga tgactttttt
    29281 tgcaatttcc tgattgttca tttgcaaaaa tctccttata taacattttg ttcttgtttg
    29341 aaagcgattt tattcgccct tacgactatt attttatcat gttttaaaaa tatgtcaagc
    29401 gttttgcaga aaaaatattc tatctactta gtgggcaagc tttagattga ttgtttttga
    29461 ctgtttcatc aggaatttct tgaaaaataa gtttgaaacc ttggttaaaa agtcttttat
    29521 ttcattatct ttcacatttt cgtaaaaatt tgattgattt cctgtattta ttttatgata
    29581 atcgtttgac agttttttct cttttttaac ataaagggct tgcatttttt atcgaaaaca
    29641 gttactatta aaagtgataa gatttttgga ggaatgaatg gaatggacaa ctgagcgtcg
    29701 ttacagactt tatcaagatt ggacgcaaga agaaattcaa catataaagg aaaatatggc
    29761 acaatctcca tggcatactc attaccatgt tgagccaaaa acaggacttc tcaacgaccc
    29821 aaatggcttt tcttactttg atggcaagtg gatcctcttt taccagaatt ttccttttgg
    29881 tgcagcccac ggtttaaaat cttgggcaca gctagaaagt gatgatttgg ttcactttag
    29941 agaaactgga gtcaaagttt tgccagatac tccattagat agtcacggtg cctactctgg
    30001 ttctgccatg caatttggcg ataacttatt cctattttat acaggaaatg ttcgcgatga
    30061 aaactggatc cgtcacccat accagatcgg tgctttgatg gacaaggagg gtaagattac
    30121 aaagattgac aagatcttga ttgaccagcc agcagactct actgaccact tccgcgatcc
    30181 acaaattttt aactttaaag gtcaatacta tgctgtcgtc ggtggacagg acttggagaa
    30241 aaaaggcttc gtccgtctct acaaggctgt caataacgac tacacaaact ggcaagcagt
    30301 tggcgacctt gactttgcta acgaccgtac tgcctacatg atggaatgtc ctaatctggt
    30361 ctttgtagag gaacagcctg tccttctcta ctgtccacaa ggattggata agaaagttct
    30421 agactacgat aatatctttc caaatatgta taagattggg gcttcctttg accctaaaaa
    30481 tgccaaaatg gtagatgtgt ctcaacttca aaacatggat tacggtttcg aagcctatgc
    30541 aactcaagcc ttcaacgctc ctgatgggcg tgctctagca gttagctggc ttggtttgcc
    30601 agatgtttct tacccatctg accgttttga ccaccaagga accttctctt tggtcaagga
    30661 actcactatc aaagacgaca agctctacca gtatccagtc gctgctatta aggaccttcg
    30721 tacttctgaa gaagccttct caaaccgttc ccaaaccaag aacacttacg aacttgaact
    30781 caacttggaa gctaatagcc agagcgagat tgtcttactt gctgataaag aaggtaaggg
    30841 actttcaatc aactttgacc ttgtaaacgg tcaagtgaca gtggatcgta gccaggctgg
    30901 agagcagtat gcccaagaat ttgggataac tcgttcttgc cctatcgaga atcaggctac
    30961 tactgctaca atcttcatcg ataactctgt ctttgaaatt ttcatcaata aaggagaaaa
    31021 agtattttct ggtcgtgtct tcccacatgc ggaccaaaat ggtatcctga tcaaatctgg
    31081 aaacccaact ggaacttact atgaattaga ttatggtcgc aaaactaact gatgtcgcca
    31141 aacttgcagg cgtcagtcct actaccgttt ctcgggttat caataaaaaa ggatatctat
    31201 ctgagaaaac catccaaaaa gtcaatgaag ccatgcgaga attgggctat aaacccaaca
    31261 acctggctcg tagtctgcaa ggaaaaccag ctaagttaat cggcttgatt ttccccaata
    31321 tttccaatgt tttctatgca gaattgattg ataaattgga acaccaactc ttcaaaaatg
    31381 gttacaagac catcatctgc aacagtgaac atgattctga gaaggaacgt gaatacatcg
    31441 aaatgttgga agccaatcag gtggacggca tcatttctgg tagtcacaac ctaggaatcg
    31501 aagactacaa tcgtgtgaca gcgccgatta tttcctttga ccgaaaccta tcgccagaca
    31561 tccctgtcgt ctcctctgac aactatgctg gtggggttct tgctgcccaa accttggtca
    31621 agacaggtgc ccagtctatc atcatgatta cagggaatga caattccaat tcgccaaccg
    31681 gactgcgcca cgctggtttt gcatccgtac tcccaaaagc tcctattatc aatgtttcca
    31741 gtgacttttc tcccgtcaga aaagaaatgg aaatcaagaa tatcttgacc cgggaaaaac
    31801 cagatgccat ttttgcttcg gatgatttga cagctattct ggtcattaaa atcgctcaag
    31861 aattgggcat ttctgtccca aaagagctca aggtcatcgg ctatgatggg acctacttta
    31921 tcgaaaatta ctaccctcaa ttggctacta tcaagcaacc tttggaagag attgcttgtc
    31981 tcactattga tcttctcttg caaaagattg aaggcaagga agtcgccaca actggttact
    32041 tcttaccagt tacgctatta ccaggaaaaa gtatttaaac acaagaaaac tcagaccgat
    32101 tcgtctgagt ttttatgatc ttaaattttc gagatagcgc tgggctgtct ctaggttaaa
    32161 ggttttatct gagatgaggc gctctactag gggagcaact tcagattcac tagccccagc
    32221 taggagagct agggatttgg cctgtagttt catgtggcct tgctggatgc ccgtacttac
    32281 caaggctttg agggctgcaa aattttgagc aagaccgatg gacacgataa tctgggctaa
    32341 ttctctggca gaaggatttc ctagtagatc atgactgaga gctacacgtg ggttgaggcc
    32401 gatagagcca cccttagtcg ctacaggcat gggcagggtc atctcaccga ccaattcttc
    32461 tctttcaagg tccagcgtcc agcagctaag accttgatag cgtccatctc gactggcaaa
    32521 ggcatgggcc ccagcttcga tggcacgcca gtcattacca gtggcaatca aaatcgcatc
    32581 aataccatta aaaattcctt tattatgagt agcagctcgg taaggatcag cctgcgcaaa
    32641 ctgactagcc aacgcaattt tctccgcaat ctctcgtcct tgatcctttt ggcggctcaa
    32701 gtagcgaaag gcgatgcgac agcttgcagt caccagagaa tcagtcgcgt agttggacag
    32761 gattcccatg agactctgtc cctgactgag ttcttctaag actggtttca aggcttccag
    32821 catggtgttg agcatattgg cacccatggc ttcctgggta tcgacatgaa tataaacaac
    32881 gagaaagtct ggttcgcctt ttatctgctc gacatgcaga tcacgcgccc cacccccacg
    32941 tttaacgata gaaggatagg cttgattggc aagctccaag agctccgctt tcttgctggc
    33001 aatcttctct tgcgctagtt taggattagc aacttgataa agggctacct gcccaatcat
    33061 ctggcgctga tggacttgtg cagtaaaacc acctgcacgc ttgatgattt tgctggcata
    33121 gctggccgcc gcaaccacag agggttcttc tgtcacatag ggaacggtgt attcctgacc
    33181 gttgacaagt acctccggaa ccagcgaata aggcagagaa aaagttccca ccacattctc
    33241 actcagctgg tctgccacag tcacactcat ctgttcatcc ttctccagac tagcttgtct
    33301 ctcaggacta aggagcgcct gagcttttag cagctcgagg cgctcttggt atgatttttt
    33361 agaaaatcca ttccaactta tcttcattat ttttcaacct tgctataacg gcgttggtgg
    33421 tcaagaattt caaccaaggc aaaatcttga ttttcatagc cagcaaactg ggcagagttt
    33481 gtttcatcca agtttacttc ctcaaaaaag accttttcat agtctgcaac ggatagggca
    33541 gttcgttggt tgagcttgtt caagcggtct ttatccaaat aagcttcata tccttcaacc
    33601 aattcaccac tgaagaactc agccacagct ccacttccgt aactataaag ggcgatttta
    33661 tccccagctt tcaagctatc tgtattttcc aagagagaca aaagtccaag gaaaagtgaa
    33721 cctgtgtaga tattccccac cttttgactg tagagaatag actggtcaaa atgcttttgt
    33781 aagaggtctt ttttctcttg aggcaggctc ttatccatga tttttttcaa gccttttagc
    33841 gctaatttag gataaggcaa gtggaaacaa acagccgcaa aatcatccaa agtaagctgg
    33901 tagcgttttt gatattcaag ccaagtcgtt ttcaaactat ccaagtattg ttgggtagaa
    33961 tagacaccat ttacataagg agttgtcgag taatttggtc gccagaaatc catgatgtca
    34021 cgggtctgag ctacattgtc attattaaag gccatcatgc gtggattttg tgtaatcaac
    34081 atagctacac ttccagcacc ttgagttggt tctcccggag tttcaatacc atatttggca
    34141 atatcactgg caatgaccaa gaccttggac tccggagaat tttccacatg caatttggca
    34201 taatggaggg cagctgtcgc cccatagcag gcttctttaa tctcgaaact acgagcaaag
    34261 ggctggatgc ccagcaagcc atgcacaaag acggccgcag ccttactctg gtcaattcct
    34321 gactcggtag ccacaatgac catgtcaact tcttgtcttt cttgctcagt taaaatagag
    34381 tcactagcac tggccgccaa ggtcacgatg tcctcagtta ggggcgcaat actcaattcc
    34441 ttgagtaaga gtcctttact taatttttca gggtcaattc ccctcgcttc tgctaagtct
    34501 tgtaatttca agacatattg actggtcgca aaaccaatct tatcaatacc gattgtcata
    34561 tttacctctg ttttatcatt catgtaaaaa atcgttctat actattttat cacaaatggc
    34621 agtaaaagag agaaaaaaga cttgattcac caaatcaagc ctcttattgg tcatcatttt
    34681 aaagaatgat tagttgctag agagttcacc gatataagta gctttataag ctccattcac
    34741 agttatcagc tcctggagga tcaaatttcc tgagtaaatc cttcccatct catctacaaa
    34801 tttttgataa aactgactga tcggaatttc tctgacatcc ttatcaaatg tcttatcaag
    34861 tgttttacta accttctcag caatcaattg atgctcttgc catccacttt gaaactctga
    34921 gcccgaacta gaaaccatga ctgggataaa caacaaggtc agtagattta cagacaataa
    34981 ggaaagtagt agacttcctg caaaactaga atc
//
LOCUS       DBEJAQ010000021        34782 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.021, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000021 DBEJAQ010000000
VERSION     DBEJAQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.021"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(1..796)
                     /locus_tag="ACL297_001058"
                     /pseudo
     CDS             complement(1..796)
                     /locus_tag="ACL297_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(1170..1319)
                     /locus_tag="ACL297_001059"
     CDS             complement(1170..1319)
                     /locus_tag="ACL297_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
                     /protein_id="HGR0071336.1"
                     /translation="MEKFNFKNNIGQENKLLQIEIYKFTNFCKLQNYTSVNIFSKDIF
                     EAIVN"
     gene            1284..1403
                     /locus_tag="ACL297_001060"
     CDS             1284..1403
                     /locus_tag="ACL297_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGR0071337.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(1499..2845)
                     /gene="glnA"
                     /locus_tag="ACL297_001061"
     CDS             complement(1499..2845)
                     /gene="glnA"
                     /locus_tag="ACL297_001061"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HGR0071338.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2882..3238)
                     /gene="glnR"
                     /locus_tag="ACL297_001062"
     CDS             complement(2882..3238)
                     /gene="glnR"
                     /locus_tag="ACL297_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HGR0071339.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYENQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(3315..3842)
                     /locus_tag="ACL297_001063"
     CDS             complement(3315..3842)
                     /locus_tag="ACL297_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGR0071340.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            complement(3978..5174)
                     /gene="pgk"
                     /locus_tag="ACL297_001064"
     CDS             complement(3978..5174)
                     /gene="pgk"
                     /locus_tag="ACL297_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGR0071341.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(5264..10174)
                     /locus_tag="ACL297_001065"
     CDS             complement(5264..10174)
                     /locus_tag="ACL297_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HGR0071342.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKTKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYRFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNK
                     ALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNTVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            10467..12097
                     /locus_tag="ACL297_001066"
                     /pseudo
     CDS             10467..12097
                     /locus_tag="ACL297_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
     gene            complement(12656..14263)
                     /locus_tag="ACL297_001067"
     CDS             complement(12656..14263)
                     /locus_tag="ACL297_001067"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HGR0071343.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDTEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(14653..15240)
                     /gene="rpoE"
                     /locus_tag="ACL297_001068"
     CDS             complement(14653..15240)
                     /gene="rpoE"
                     /locus_tag="ACL297_001068"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HGR0071344.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(15368..15817)
                     /locus_tag="ACL297_001069"
     CDS             complement(15368..15817)
                     /locus_tag="ACL297_001069"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0071345.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(15822..16211)
                     /locus_tag="ACL297_001070"
     CDS             complement(15822..16211)
                     /locus_tag="ACL297_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGR0071346.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(16239..16889)
                     /locus_tag="ACL297_001071"
     CDS             complement(16239..16889)
                     /locus_tag="ACL297_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGR0071347.1"
                     /translation="MRDKQTFLMKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIR
                     GDLPDYLTILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(16879..17442)
                     /locus_tag="ACL297_001072"
     CDS             complement(16879..17442)
                     /locus_tag="ACL297_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGR0071348.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(17607..17841)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(17895..18398)
                     /locus_tag="ACL297_001073"
     CDS             complement(17895..18398)
                     /locus_tag="ACL297_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HGR0071349.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(18591..19421)
                     /locus_tag="ACL297_001074"
     CDS             complement(18591..19421)
                     /locus_tag="ACL297_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGR0071350.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(19406..21088)
                     /locus_tag="ACL297_001075"
     CDS             complement(19406..21088)
                     /locus_tag="ACL297_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071351.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(21190..21447)
                     /locus_tag="ACL297_001076"
     CDS             complement(21190..21447)
                     /locus_tag="ACL297_001076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071352.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(21454..22002)
                     /locus_tag="ACL297_001077"
     CDS             complement(21454..22002)
                     /locus_tag="ACL297_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HGR0071353.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(22017..22865)
                     /locus_tag="ACL297_001078"
     CDS             complement(22017..22865)
                     /locus_tag="ACL297_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HGR0071354.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            23053..24402
                     /gene="trkA"
                     /locus_tag="ACL297_001079"
     CDS             23053..24402
                     /gene="trkA"
                     /locus_tag="ACL297_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGR0071355.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            24406..25845
                     /locus_tag="ACL297_001080"
     CDS             24406..25845
                     /locus_tag="ACL297_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGR0071356.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(25970..26371)
                     /locus_tag="ACL297_001081"
                     /pseudo
     CDS             complement(25970..26371)
                     /locus_tag="ACL297_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
     gene            27769..28053
                     /locus_tag="ACL297_001082"
     CDS             27769..28053
                     /locus_tag="ACL297_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071357.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            28380..28598
                     /locus_tag="ACL297_001083"
     CDS             28380..28598
                     /locus_tag="ACL297_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071358.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(28965..31289)
                     /gene="pflB"
                     /locus_tag="ACL297_001084"
     CDS             complement(28965..31289)
                     /gene="pflB"
                     /locus_tag="ACL297_001084"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGR0071359.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIRWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            31683..32744
                     /gene="dinB"
                     /locus_tag="ACL297_001085"
     CDS             31683..32744
                     /gene="dinB"
                     /locus_tag="ACL297_001085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGR0071360.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(32891..33736)
                     /locus_tag="ACL297_001086"
     CDS             complement(32891..33736)
                     /locus_tag="ACL297_001086"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGR0071361.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            33991..>34782
                     /locus_tag="ACL297_001087"
     CDS             33991..>34782
                     /locus_tag="ACL297_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGR0071362.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIETTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILA
                     EIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRW"
BASE COUNT        10325 a         7447 c         6390 g        10620 t
ORIGIN      
        1 cttgggaaac aatagaaatg tccaaatctt tctttttaat cttgccttct tcaaagagtt
       61 tttgtttttc tgctcgtatt ttttcaagta aaacttcgac tgattcatca tttgggtctt
      121 gttcaactaa ttttccttgc atagcatatt gaagaataga tttttttagt ttatctggaa
      181 attctttatc tagctgttct agtctattat aactttcagc atattcatct actttttcta
      241 aagctgattc gattgcttct attatgcgtt gttgttcgga tagtgggggg agagggataa
      301 gaatagaagc aactttatca ctattcaagt ttttcacaac agctccacta attagagata
      361 gaaattgaga ataaactaca tttgatgaaa gaatatagaa taggtaatct ttatttaatg
      421 agttttcata gttcgaaata gccaaccatc catcgtgtat tgcaccatca acattcaaaa
      481 tataaggtct accaaaactc atagaattag ttaacaaaaa tgtacctttt tttacaaatc
      541 tagttttgtt aagccctgat tttttgattt tttctttaac attatttata tacttttcac
      601 ccttttcagt atcacctatt tttatccaat ttattccatc tacttcagaa gtaagataat
      661 ctttgattgg tcgtggagag ccacctctga caatttcaac caatgtagaa aacctcaccc
      721 actcccaagt atcaggaata tcataaggaa catcaatttc ttgagtgctt ccatcagcaa
      781 acttcccata atgtttctta tgtgcttcaa gtatataaaa aggcgtaaaa atacgcctat
      841 agataatggg gttgaaatag gtttattgtt gatgagattg tagataattc aattttttac
      901 ttccaatcga atattcaaaa cctccacctt ttctgcctgt aattgttcat cataaaattc
      961 aatatcttca ggattttccc cttggcaacc tcggcagaaa tattcttccg ctcgatcagg
     1021 attcaaaaat cgacaagcac aaacaaaaca gtcgccatca tcatttattg agataatata
     1081 gtagattgaa ataagatgta aacaaatcga ttaggaaagt taaattagtt tctagaaatt
     1141 tttagcagat gtagtgtact attctagtct caatttacta tggcttcaaa tatatctttc
     1201 gaaaaaatat ttacagatgt gtaattttga agcttgcaaa agttagtaaa cttgtagatt
     1261 tcgatttgaa gtaacttgtt ttcttgcccg atattgtttt tgaaattgaa tttttccata
     1321 gtgactcctt aattttcttc tacacgtctg atgataaatc taattcgcaa aagagtcaag
     1381 aggatttttc gaaaaataaa tagcgaccga aatcgctatt ttaagggtta taggtatttg
     1441 atggcttaga ctgctgtgtg actgtttacc cacaggcaat ctttcttcta tattagtatt
     1501 agtaaaggtc taaataatta tcaatttccc attgtgaaac gaaggttgca taacttgccc
     1561 attcgattcg tttggcttca aggaagctag tatagatgtg atctccgaga gcagctttaa
     1621 ccacttcatc ttctgtcaaa gctttcaaag cgttgtgaag agttgatgga aggtctgtaa
     1681 taccagcttc cttgcgctct tctgctgtca tgatgtagat attttcttcg ataggagctg
     1741 gtgcttcgat tttattttca ataccataca aaccaacttc caaaagaaca gccatagcaa
     1801 cgtaagggtt cgccattgga tccactgaac gcaactcaag acgagttccc ataccacgtg
     1861 aagcaggtac gcgcacaagt ggcgaacggt tacgaccagc ccaagcaatg taaacaggcg
     1921 cttcataacc tggaaccaaa cgtttgtatg agttaactgt tgggttcatg atggcagtat
     1981 agttgtaagc atgcttgatc aaaccgccta ggaaatggta agctgtttct gacaactgca
     2041 ttccttttgg atcatttgga tcaaagaagg cgttatttcc ttctgcatca aacaaggaca
     2101 tattacagtg catacctgag ccagcaatac caaattttgg cttcgccata aatgttgcgt
     2161 aaagtccgtg tttgcgagca atggttttaa caacaagttt aaagatttga atcttatcac
     2221 aagcacggag aacttcatcg tacttaaagt caatctcatg ctgtccaacc gcaacctcgt
     2281 ggtgactcgc ttctacttca aatcccattt tggtcaagac attcacaatc tcacgacgtg
     2341 tgttgtccgc aaggtcagta ggtgccaaat caaagtagcc acccttgtca ttcacttcaa
     2401 gtgttgggtc cccattttca tccaacttaa ataggaagaa ttctggctct ggaccaaggt
     2461 tgaaggattt gaatccaact tcttccatgt gacgaagagc tcgtttcaaa ttaccacgag
     2521 ggtcacccgc aaatggttca ccttctgttg tataaacatc acagatcaga cctgcaacac
     2581 ttccattttc atctccccaa gggaagactg tccatgtatc caagtccggg tacaagtaca
     2641 tatccgactc attgatacgt acaaaacctt caatagaaga tccatcaaac ataaccttgt
     2701 tcgacaagac cttatctaac tgttcatctg tagcaggaat ttcgacgttt ttcatggttc
     2761 ccaaaatatc tgagaacata agacgaataa aggtaacatt tttttccttg acttcacgac
     2821 gaatatctgc agctgtgatt ggcataagtt ttctccttaa tctatgacta cttgcggttg
     2881 cctaaccgcg accaaaaggt gactgtactg aagcaaaacg cccctgttgg aggagttcat
     2941 tgtgaagtgc acgacgtact tcagtctgac taaccgcttt cttggatttc gcttcacgtt
     3001 cagcatattt tttcttaatg gcagcgatat tataaccttc agagatataa tctttgattt
     3061 caagcagacg atccatgtca ttcaaggaat acatgcgacg atttccttcg tttcgatcgg
     3121 gcttgatcaa ctcttgattt tcataataac gaatctgacg cgccgataga tcggtcaact
     3181 tcataacact gccgatagga aaaacagcca tatttcggcg aaattctttt tccttcattt
     3241 acaatttcct tctttctgtc tattatagtc taaaaaaaga caaacgtcaa ttaataatgt
     3301 tataaaatgt aacattattt ttcttttttc tctaaaaaga gacgaatacg atcaatatcg
     3361 taatttacga taattgcgac aaaaactccc ataaatgttt ctaatacacg cacaaacacg
     3421 tacaaaattg tctcaccact tggaattgat agggtaataa ttaacatagc tgctacacca
     3481 ccaataaccc ctgctttgtt attcatggct acatttgtca taatggttaa catggtgcag
     3541 attggaacaa ctaccaaggt cacccaaaag gcttcgtgga aaaaggtatt taataagaag
     3601 aagaccaagg catagagtcc accgatacta tttcctagaa tacgcgaagt cccaaaatga
     3661 acactctcat caaaactctc cctcaggcta aaaacggctg tcaaagcacc aatttgaaga
     3721 cctttccagc caaaaaagcc aaaaatcaag agaactagaa aaacagcaat acctgtttta
     3781 aaggttcgca taccaagttt gaactgggat ttatcgaatt tatatttttt aaaataactc
     3841 ataatctcaa ctttctattt ccattttatc ataaatcggt gatttttatg agtaatagtt
     3901 gagaggaagc gtttttattt taagcaaaag aaaagaggaa ctttcatccc tctcttcttt
     3961 gatttgttta taaaatctta tttttctgtc aaggctgcaa gtcctggaag aaccttacct
     4021 tcaagaagtt ccattgatgc tccaccaccc gtactaatcc atgagaactt gtctgcacgg
     4081 ccaaggttaa tcgctgcggc agctgagtca ccaccaccga tgattgattt aactcctggt
     4141 tgtttcacga tagcgtccat cacaccgatt gtaccagctt ggaaatctgg gttttcaaat
     4201 acacccatag gtccgttcca tacaactgtt ttggcaccag tcaaagcttc gtcaaatttg
     4261 gcgatagatt ttggaccgat gtcaagacca aggaagcctt cagaaactgc ttcaccttca
     4321 gtgtcacgca cttcagtgta accagcaaat gcgttagctt cttttgagtc aactggcaag
     4381 atcaatttac catttgcttt ttcaagaaga gctttcgcaa catccaattt gtcttcttct
     4441 acaagtgagt taccgatttc gataccttgt gctttgtaga atgtgtaagt catcccacca
     4501 ccgataagga ctttatcagc tttttcaagc aagttttcga taacaccgat cttgtctgaa
     4561 acttttgaac caccaaggat agccacgaat ggacgttctg gagtttcaac tgcttcttgg
     4621 atgtaggcaa tttcgttttc aagaaggaaa ccagcaactg ctttttcaac gtttgctgag
     4681 ataccaacgt tagatgcgtg tgcacggtga gctgtaccga atgcatcgtt tacgaagata
     4741 ccatctccaa gtgatgccca gtatttacca agttcaggat cgtttttaga ttctttcttg
     4801 ccgtcaacat cttcgtaacg agtgttttca accaagagaa cttgtccatc ttcaagagcg
     4861 ttgattgccg cttccaattc agcaccacga gtgacacctg ggaaaacaac atcttgacca
     4921 agttttgctg ccaagtcagc tgctacagga gcaagtgatt taccagcttt atcagcttct
     4981 tctttcacac gtccaaggtg agagaaaaga attgcacgtc caccttgttc gatgatgtac
     5041 ttaatagttg gaagagctgc tgtgatacgg ttatcgttag tgattacgcc atctttcaat
     5101 ggtacgttga agtcaacacg aacgaggact tttttacctt tcaagtcaac gtctttaaca
     5161 gtaagttttg ccatgttaca aaaactcctt tatttatttt attcgaaact attatatcac
     5221 actttagaaa tataaggaaa gatttcacaa gatttttcga tatttaatct ttatcttctc
     5281 ttttccgtct caaggtcaag gctaaactag caagaccaag gctggctaag cctgctatta
     5341 gggcttcatt agcatcagct gttcctgtat ttggcaattg tttgcttgct tcttctgatt
     5401 taactgctgt attttgagct ggtttttcct gtgtagctac tactgctggt actgttttta
     5461 cttttgttcc aatttcaaca atttctggaa tcgcttcttg gatgacttct gttgaacgaa
     5521 gacgacgctg accgttttca tcaacttcaa agacatgtct taacagtcca tctcgtccct
     5581 gtatgatgac tcgttgttcc ccaactagca tctcagcatt ttcatgctct tgacgatgaa
     5641 aggcaacctt ttcctcttga acttccaatt taggagcctt ttgttgatca gaagccggtt
     5701 gataagtagg ttttggaagg actgtgtttt gtggtgctgg tttagcaggt tgtggttttt
     5761 cagcagattc ataaacaaag gcataatgag taaagtgagg tgctgtaaag ataacatgac
     5821 tatccgtttg ttcaaaagcc aattctactg cctctttgcc ttcaggtaag aaaaatactt
     5881 tcttaacttt cttatctttt tcaattggga ttttcacaat agaagcataa gagagatcaa
     5941 catcttgacc tttttcatcc aaaccttcta tttcaaagac atgagcatct tctccagcaa
     6001 agtatttctt ctcttcagca cttgcttgaa cacgctctac tttcaaaccc ttgatgacag
     6061 tcttctcttt atttgagaag tgtacttcaa cacctgtttt actatcagaa aagactgctg
     6121 gcttgtcttc tttcaataaa gcaaccagat cttttagact ctgtttcgct gttgcaagct
     6181 cttctggact caaatctact ttttcaacca aggccttgtt tttctcaaga agcggagcta
     6241 gggcagaacc tgaaggatgg tttggtttgg cagccaagag ttcttgaacc tcacctgtta
     6301 aacgatcaag ctctgtcttt tcttccttaa atttctcttg accattcaga gctttatgag
     6361 cttctgtcaa tttattaagt cggtcattga cttgatcttg ggtgctagtt tcatggtctt
     6421 tcaacacttg ttcggccgct tcaattgctt tcaagaaagc ttcttttact tctggtgaag
     6481 caaagttata gaccaccttg ttcttagcag cttcaacttc tgctaatttt tgtttgaggt
     6541 actcatcgtt caatgctgct tttggagata ccaaaacatc aaagttgact tcatgacctt
     6601 ggtaacgaag cgtcaaggtt tgacgaccag tcttttgagc atcgtaacca gaaatttcaa
     6661 ctccctcatc agtaaaggaa tgttcttcca tggtgtcatt gctataagcc actgcaaagc
     6721 gtccttcaga caagtctaag ctatcaccaa ctaggtaatc ttttttcggt tcctgactta
     6781 cttcaattcc caaaatagtt ttcggacttg cttcgtcttg gccagtgaca gtcactgaca
     6841 aattagcatt tactggttgt cccaaatatt ggagagtaag attctgttct cccttatgat
     6901 gcgtatcaaa acctgatact gatacacctg cgtgagttag gcgaatgagt tcgtcctcag
     6961 ttcctccttc atactgaact cgaagaacac cacctctaag gtccaaatcc tccccttcgg
     7021 cgtagcttgt tttcttaggt cctgtttcta ggctgactga cttgattctt ctgtcatctt
     7081 ttggaacgga aactgctagg acattactaa tttccttgcc tggcaactgg gtacgataat
     7141 aaagaagacc agattgattt gtcaaatcca atggtgcact tgctaggtct cctccaactt
     7201 cgctcttcaa ggttgcgagt ggagtttgag aatttggatt atcataaacg gtaatagttg
     7261 ctccagccgg tacatctgca aagccaactt gtaccttgtt attgcctaga gaacgggctg
     7321 cagccttggc catcggaata ttgacactct cagtatcaag cttttcatac attttccagt
     7381 tatagatacg aatagccttc catggagttc cattgtcaga agtgacaaca ttcaagcgcc
     7441 agtcttgagc agtgattggt ttatcaagag tgatatctgt cacgtgtgct ttgttgccac
     7501 ggacttcctt agctagcttc cactcaccat ctgcatcttt ataataaagg tcaaagtctt
     7561 tggtgttcat caagccatcg ttaacagact caccaccagc ccctgcatga tccatgaccc
     7621 atctaacaac ggtacgtggc ttggtcaaac gaatatccac actaccactc aactgagctg
     7681 aagaccattt atctgacaag ctagtaatgg taccgttcaa cataccttca ataccttctc
     7741 caccttctgt atctgggaag gtacttccga taacagtagc ccctggaacg atgttttctg
     7801 ctagaggtct tggaagactt gtgtcttgga cagtcatccc ccactcaaag gttgtggttg
     7861 cagcttctga acgaactcca tttttaccga ctgctacaac cttcagttct tgagttgtac
     7921 cctgagcact tgctgagcgg ctaacttttg gtagataaat agttgtagaa gatgagccag
     7981 ttagtaattt ccagctgtct ccatcttttt cataaacttc atagaaatct gcatccttgt
     8041 tgcctttaaa ttgcacaact gcttccgctt cttgggcatt tttaagagat tgtttcacta
     8101 cagaaaagct tgtcggcgat tgtggctctt ggtgattgtc cgagatagtt aattgtccta
     8161 ggttgaaccg ataatctttt acagcacctt catgctcgaa aaatagtttg actgcataga
     8221 tggttttacc tgctagtgag ctgagatcaa attcttcatt tgtccagttg tcagaaaggg
     8281 ttagctcttt ccatgcatct gcatcatcga atttgtagtc tggagttgta gagaatgcca
     8341 tataaacttt agaacctttt cctcccttgt gggcaacacg aagtttggtc ttctcagtta
     8401 cttctaactt agtagaataa agtctcacat cctgatctgt cttaccggct acatcaccag
     8461 agaatttaag ggaatttccg ccattatagg catctgtaaa atcatattct gcacgaagtt
     8521 tttcccctgt tgaagtctgc caccagcgcc atgttggaag aacacctgat actgaacgat
     8581 aattccactc agaatcctta gaaaccttac catctacgaa ccatttttta ccatgacctg
     8641 tattaaaaga agtagtaaaa gtattaccta ccgctggcgt acggtccgca actaggttag
     8701 caataccata ccaatctttg tcacctggtt tttgaccagt agggtctcct tgataacctg
     8761 taaagaagat atcttcattt ttatgataat cttcaccagt ttttcctaaa cttgtaatgg
     8821 tatctggggc aaataaacca agagaaaggc gcaatttccc attttcgtct aaaatgtcat
     8881 tccacttaac ctttgtcttg taggaaccac cctgttgcaa ttccaaacct gcaaatacat
     8941 cataaggatt acgaccaatc cagttggcag ttgcaatagt gtaatcattt ttagccttat
     9001 cccagttaaa gttagcaaag aagttatctg ccggaacctt atctccttct ggttgcatga
     9061 attggtagtt gtattctccc aaaccatctt gatggtaacg tccatagtta taggtcatgg
     9121 catcatacca agaatacttg attggatggt ttaccttagc agcatattcc ttgctataga
     9181 gcataaactg gcgcatcttt tctccaagag gtttaaccaa atctccagtt gtttcttggt
     9241 tgatgaaata gccatcatag ccataatact tggccatgtc taccaattta cgggcaattg
     9301 ggaagctacc atctgcgtct tgcttcaaag cttcagcaaa tctttcttga tctgcaatac
     9361 tattagacca gttgaagaag agtgtaccgt atacaggaac cccgttacgg tgacctgcat
     9421 caataacgtc aggagttggt acgagacctt cccagaagac cattgaatct agatattgcc
     9481 aatagtcaaa agcataggcc ttgaactctt ctccaccaac agaagcatgg tcttttgttt
     9541 tagaattggt gtttgataag gcttgaactt ttgcttcctt gctagctttt tcattgacta
     9601 aatgacctgt ccgacgtgaa gcgaggacaa cagatccgcg gttaatagca tcatcttcac
     9661 gagcacctgg ttcccatttt aacaattctt cccaagaatt aaatttgatt tcctttggtt
     9721 ttaatgtttt ttctgtattt ttagggacat ctggctggac ttctttttca gtcttattag
     9781 cttctgctgc tggcttagac tcatcccctt ggttagttgc ttcgggcttt gcttctgttt
     9841 ccttgtcaga cttaggtttt gcttctgcag atggtgtttc tgcaaccacc tcacctgctt
     9901 tttcctctgg tttagctgcg ctagctgctt ctgccttttc tagcttgttt tctctagcat
     9961 gttccccttc attctgctta tcagcttcat tggtcgaatg ctcactctct cctgaaacaa
    10021 gctctgtatt agctccgcta ttttcaggaa ctgcagtttc ttcagccaag gctggaccag
    10081 caaataaaac agcaccaatc atcagcgagc aggctcctac tgataactta cgaatactgt
    10141 aacgacaacg tctttcaaaa aatggattct tcattttctc ctcctaaaag attgattttg
    10201 ttgttttaaa gaaagcgctt tatattctgt agttttttat caaactctag aaccatgata
    10261 atcacatttt tatcctaaaa catttttaga caaaaaacaa taactgtttg cacaaatcta
    10321 aaataaggtt aaaaactctt aaaaataata cacttatcaa ttataatttt cttttacaaa
    10381 cttgacatta acacttgtta gatttacttt tatcctttaa aatagaatag gagatattcc
    10441 gcgattattt ttcggaggaa aaagaaatgt ccattaattg gcaggaaatt ttatttcact
    10501 ttttaggtgg tctggggcta ttcttatata gcatcaagac catgggagac ggtttgcaac
    10561 aagctgctgg agatcgcctt cgtttttaca ttgacaaata tactagtaat cctttctttg
    10621 gagttctggt tggtattggg atgactgctc taattcagtc tagttctggt gtaacagtta
    10681 ttacagtcgg cctggtcagt gccggtctct taaccttacg tcaggctatc gggattgtca
    10741 tgggtgctaa tattgggaca actgtcacat cctttctcat cggttttaaa ttaggtaact
    10801 atgccctacc tatgctcttt atcggtgccg tctgtctctt ttttacgaaa aatcggacag
    10861 tcaataatat cggacgcatc ctctttggtg tcggtggtat cttttttgcc ctcaatctca
    10921 tgagcggcgc aatggctcca ctcaaggatt tacaggtctt taaggactat atgattgagc
    10981 taagtaagaa tcctgttttg ggtgtctttg tcggtaccgg cttgaccttg ctaattcaag
    11041 cttcttcggc taccattggg attttacaaa acctctacgc cggcaatcta attgacctac
    11101 agggagcttt gccagttcta tttggtgaca atatcgggac aaccattaca gccatcattg
    11161 cctctttagg ggctaatatt gcagctaaac gggtagcagg agctcatgtt gccttcaacg
    11221 ttatcggaac agtcgtctgc gttatttttc tagttccttt tactgtcctg attcattggt
    11281 ttgaagctac gctaaatcta gcaccggaaa tgaccatcgc ctttgctcac ggaaccttaa
    11341 tattaccaac accattgtcc aatttccatt tatcggagct ctggcttact ttgtaaccaa
    11401 gattattcct ggagaggacg aggttgtcaa atacgaaccc ttatatcttg atgaacattt
    11461 catcaaacaa gccccatcta tcgctctagg aaatgctaaa aaagagctct tgcacttagg
    11521 aaactacgct gctaaagcct ttgacctttc ctataagtac atcattgact tggatgaaaa
    11581 agttgctgaa aaagggcata aaaccgaaga agcaattaac accatcgatg agcaattaac
    11641 acgttatctc attgcccttt caagcgaagc tctcagccaa aaagaaagtg aagtgctcac
    11701 caatatcctt gattcctccc gtgatttgga acggattgga gaccacacgg aggctctact
    11761 caatctgact gactatcttc aacggaaaaa tgttgaattt tctgatgccg ccttgaaaga
    11821 attagaggaa gtttaccgcc aaactagtga ctttatcaaa gatgctctgg atagtgtgga
    11881 aaacaatgat attgaaaaag cacgcagtct tgtagaacgt catgaagcaa tcaataagat
    11941 agaacgtgtt ctcagaaaaa cccacatcaa acgcctcaac aaaggcgaat gttcaacaca
    12001 agctggggtc aactttatcg acatcatctc acactacact cgtgtatcag accacgctat
    12061 gaaccttgct gaaaaggttt ttgcagaaca aatctaataa ccaagaagct atccatcata
    12121 attggatggc ttcttacttt ttcctaagca agactaggct gaatgaaact gaaagagtat
    12181 tctgcagata tatagtatat tgaaactaga atagtacacc tctatttcta aaacattgtt
    12241 agaaatcgat ttgactgtcc tgatcgattt gtcctgttat tatttcattt tactatagtg
    12301 gattaaatta aaaaagtata atgtattgaa tctataacag tacatcttga ctgctaaaat
    12361 atttctataa attaatttga ctttccttat agagatgttc acatcttatt tcaattcatg
    12421 atacaatcca gttcctaaaa ctttgctaga aattaatttg gctttccgaa ttaatttgtt
    12481 cagattttat ttcatttcaa tatactgata aatccattca ctagcacagg gcaagaggga
    12541 gtcgctattg atttcaacgg tagcaggtac atacagagga ctcctacctc agatatactg
    12601 caatatcatc gtatgcaaaa aacctctgag atttttctca aaggttctga ttttgctaat
    12661 tgctgttctc aaccgctgca gtaacaaagg cagtgtagag ttcttctggt cggtttggac
    12721 ggcttgacag ttcagggtga tactgacaag ctacaaagaa tttattttca ggaatttcca
    12781 cgatttctac caaacgattg tctggagaaa ctcctgaaaa gacaaaacct gctgcctcaa
    12841 actgctcacg gaaggcatta ttaaactcat aacggtgacg gtgacggcgt tgcaccactt
    12901 cttgattgtg ataagcagca gcagccttag agccacgttt caacttagac ggataaagtc
    12961 ccaaacgaag ggttccaccc atatcctcaa tatcaatctg atcacgcatg atatcaatga
    13021 tagggtattt tgtttctggt gcaagctctg cagaattggc accttcaaga cctaaaacgt
    13081 gacgagcaaa ctcgatacat gtcaactgca ttcccaagca gactcccaac attggaacat
    13141 cattttcacg cgcatagcgg atggcttgga ttttcccttc tgtaccacgt tgaccaaaac
    13201 cacctggtac gatgatcccg tccgcatcag acaagagttc tgctacattc tctgctgtca
    13261 catcattggc attgacccaa ttgattttaa cttctgtatc attgacatag ccagagtgtt
    13321 tcaaggcttc gaccactgag atataggcat cttgcaactc cacatactta ccaacaaggg
    13381 aaatcttaac ttgtttcttg aggttcatga ccttgtccac catggctgac cattctgtca
    13441 tagccgctgc tggtgcgtct aatttcaaat gatcacaaac aatttggtcc atcccttgtg
    13501 cctgcaagtt cagtggaatt tggtaaaggt gttcaacatc caacgattcg ataacggctt
    13561 ctggtgccac atcacagaac tgggccagtt tatttttaat tccttgacca gctggctctt
    13621 ctgtacgaat aaccaacata tttggttgga ttcccaagcc acgcaattct ttgacagagt
    13681 gttgggttgg tttggttttc atttcaccag cagccttgag gtaaggaagc aaggttgtat
    13741 ggatatacat gacattatcc gcacccacat ctgccttcat ctgacgaaga gcctctagga
    13801 atggcaagga ctcgatatct cctactgttc caccaacctc tgtgataatg acatcagagt
    13861 cggtcgttag agcggcacgc ttgatttttt ctttcaaagc atctgtgata tgaggaatga
    13921 cttgaacagt tgccccaagg tattctccac ggcgttcttt acgaagaact tcactgtaaa
    13981 ttttcccagt tgtcacgttg gaatatttgt tgagattgat atcgataaaa cgttcatagt
    14041 gacccaagtc caaatctgtc tcagctccgt catctgtcac aaaaacttcc ccgtgctggt
    14101 aaggactcat ggttcccgga tcaatattga tataagggtc aaacttttga atggttactt
    14161 tgagaccacg atttttcaag agacggccta gactcgctgc cacaatccct ttcccaatag
    14221 acgataccac accaccagtt acaaaaatat atttcgtaga catagattcc tctttctaaa
    14281 atgctcaagg tcttgttaac tacgagggga tatagaaaac aagaccctac taataagaat
    14341 aatctgggac gactgcacca gattcacaac taatactctt cgaaaatctc ttcaaaccgc
    14401 gtcaacgtcg ccttgccgta gatatgtgtt cctgactttg tcagtcttat ctacaacctc
    14461 aaaacagtgt tttgagcagc ctacggctag tttcctagct tgctctttga ttttcattga
    14521 gtataaaata caagaagata acttcttgaa aaaacaaaaa tagctcccta agaactaggg
    14581 agccccgacc tctaaaagag gtgcccaaac aatatgatac ctaaaaatag gataattgtc
    14641 aatagctaac tcttattcct cgctggtttc agcgtcgtca tctgaaaact cgtcgtcgtc
    14701 ttcgttgaga tccacatctt ctcccaagtc atctggagcg atttcgttga tttctgcatc
    14761 ataagcttcc acttcatttt tttcatcatc tggattttca tcatcgtatg aaagagctgg
    14821 atctgcttcg tatgcatctt cgtcttctgg atcatctgca ttgtagtcaa tggcatctga
    14881 atcaccatcc ataaaggcat tgacacgttt tttcttagct tttggtgcta cttcatcgtc
    14941 gtcattttct tcaagagcga tgatttcttc gtcgatttcg tccacaccat accatgaacg
    15001 aagaccccat ttgttgtccc caagtgagat gaagctaccg tcaaagttca actctgtgta
    15061 gaacagaggc aaagcttcgc ggatatcgct gtttgatgtt ccaaggtagt tttgaatttc
    15121 gtttacaaga tcgctaaaat gcatctcatg atcgcgacca cgaagttcta atatagcacg
    15181 cgctacctca atcatagata gttcactttt ttcttgccca gcaaatactt ctaattccaa
    15241 agcgtttctc ctcatttata ctactatcgc cagagcgaac agactctgac ctcattttat
    15301 catttactct ttattttacg ataattttgc ggaatagtca aaggttaagg gggagaaagt
    15361 ggcaggatta gactaattcc aatataaaac tccttccttt ttctgttgct ccattttcca
    15421 caaatccaag cgacttgaaa cacctcctag aagcatgatt gtaggtgtag atttccttga
    15481 ctctcaattc tttccatcct tttactcgag ccaattcaat caaagcactt agaatctttt
    15541 ttccaagtcc tcgatgttgg taagcggaat tcccaatcac aatggggaga ttatcctgag
    15601 atagtgtaat atccccaatt ggaaaccatt ctcccttctc cttgacttca atccaaaaaa
    15661 gctcaccatg ccgatccaaa taggaataca tggcttccaa ggtcgcttga ctgtaaggaa
    15721 gcttcacccc atctacgagg taaaccaagt tcacatcctg ataccaagca aaagcttcct
    15781 cacaatgatt gaccttataa taaggaacaa aacacaatgc actacctttt caacaaaaga
    15841 gttgctgctt gattaaaacc atcacaccag ttataccatt ttgcttcata cccatcttga
    15901 gctaggatac gatcttctaa atcaaaaaca gagtaaatct ttctttcctc gcaagcttgc
    15961 gcatagagat gatatagttc atcaccacca tctctatccc actcagcaga aatcgtatcc
    16021 cgatctgcca ataaagtctg ataagccctg tgatgcccat ctgtaatcag caaacaatct
    16081 ccaaaggcaa gaatactgat tggatcgact tggattgttt ctgccgactg gtaaagcatc
    16141 tgaatatctt gcaacttctt ttctgataaa tatagttgag tcggatgaag atctgctatg
    16201 ttgactttca tttctttctc ctcaagggaa ttcgatactc acttctgttt gcctttaaat
    16261 cgccattgga agcggagctt gtcatagaag ggaaactcga taaacaggac tcccaagccc
    16321 acacagagac tggcaaggac gtctgatggg taatgaactc ccagatagac tcttgatacc
    16381 agcacactga ctaggtagag gccaaggacg atttgtacga tttttctcca gactggatct
    16441 ttaatccgct gactaagaat aacaatcaaa gtacctacca tcaaggttac agccagagaa
    16501 tgcccacttg ggaaggaaaa tcccttctcc tccaccagat gtaagatatc tggccgtggg
    16561 cgctggtaga tatttttaaa ggtcacgatt aaaagacctg ccaaagccag atttcccagc
    16621 atgaagaaac tttctatctt ccatcgctta cgataaaaga caaaagctgt aatgacaacc
    16681 caagtgataa tcactgggat atcaatcaga cgtgtgaggg ctcgaaaaag aatagtcaaa
    16741 taatctggta agtctcctcg aatggcagtc tgaatcgatt ggtcaaaatt gaccagcgtt
    16801 tcagggtaaa atttgaccat gtagccaaga ataacgaaaa gtaaaagggc aaaactgccc
    16861 ttcattaaaa atgtttgttt atctctcata atgttttaag gttggtttca agagaacata
    16921 caacaaccag aatgaaacgg aaaagataac accttcaatc aagttaaaag gtaataccat
    16981 ggtcattagg tagttggaaa gtcccaaaat ttttccaata tcaaagttag caaacttagc
    17041 gtacaaagga acagcataaa catagttgag aaccaacatg gccaaggtta aaccaatagt
    17101 tccagctaga gagcctagta ggaaacgaag ggttgtccgt tcctttttcc aaatcaaagc
    17161 aaatacgatg acaaaaactc ccaaagctac gatattcatc ggcaaaccaa tgtaagtatt
    17221 cactccttgg ctgttaagaa gcaacttcaa gagtgagcga agcaagagca ctcctagagc
    17281 agcaggcaaa tccatgacca ccagacccac aaggactggc aagatactaa attcgatctt
    17341 gaggaaagat gccgctggta aaagcggaaa atcaaagtac atcagcacaa atgagatggc
    17401 tgatagaatt gcaatggtcg aaagtcgacg tgtgtttgtc ataacaggtt cctccaattt
    17461 tctataaaat cagaagaagt tggaaaggat tcctctatct attctcactt tttatatccc
    17521 aaaagttccc tcttactcta ttaaagaaaa acaaagcaag tggttacaat ccggctataa
    17581 atctatcaaa acagacaaag ctattctttc gtcttctccc atccagacta tactgtcggt
    17641 tgtggaatct caccacatca gcttgcgctc gcggacttct ttaaagggaa agaaatagag
    17701 atttttcttt tagactaggc gacaagctgt aggcataact tctggttagg caaagcttgt
    17761 caacaaagta taaaaggaaa agatcatttc ctagttaaag tcaccgccgg tcgggaatca
    17821 caccctgccc tgaagactct tttatcataa caaaaaacgc ttgcaagcgc aagcattttg
    17881 ttcattttca ttttttattt caatttatcc acttcatagg tatggacgag ctcaagacct
    17941 gcaaagttct gctggcgaag agcctcatag acaatcatgc agacggtatt agacacattg
    18001 agactgcgga catgttcatc attcatggga atacggagag ccttctcagg atgttctcgc
    18061 ataaaatctt caggtaagcc cttgtcttca cgtccaaaga gaaaataatg gtcctcgtca
    18121 gtcgataaat ccacctcaga atagattttt tccgcgaatt tcgaaatcag atagagtttt
    18181 cccttcatct gagatatgaa atcttctaaa ctctcgtaaa aataaatctc tagcttatcc
    18241 cagtaatcca atccagcccg cttcatcttg cggtcatcaa taggaaagcc cataggcttg
    18301 atgatgtgga ggggagaatt ggtcgcagcg caagtacgcg cgatattgcc tgtattttgt
    18361 ggaatttgag gttcaaataa tacaatgtga tttgtcatga cttgcttcct ttcaccattg
    18421 caaaaaaata gccacactgc ccggagtcaa gctcagcaaa cagcgtggtt aaggcatcgt
    18481 taacttacct cacaacaggt ttgaagtaaa tcagcgaaac tactttctta gtataacact
    18541 ttcagaatca ttgtcaatag aaatgacttg attttttcaa ttttttcaag ctatttccaa
    18601 gggttgtaaa atcgtccctg attctgcaag ataagtagta aactaactac taaaaacaag
    18661 gttgccaaga gcaaggtaat atagtctcct tttttcaagg cctgataact ataccatgtg
    18721 cgttttttct ctttcccaaa gcggcgaagc tccatggcag tcgcaatggt atcaatgcgt
    18781 tctagcgagc taaaaatcaa gggcgtaata atgagcagat tgcctttgat tcgttgcata
    18841 agagaagctt tcttggataa ttccatccca cgcgcctcct gagacatctt gatagtaaag
    18901 aattcttcct gcaaatctgg aatatagcgc aaggtcaggc tgacagaata agcaatctta
    18961 tagggcacac caatttgatt taaactggaa gcaaactgac taggatgggt tgtcatcaaa
    19021 aagataatag ccagaggaat ggtgcaaaga tacttaatgg ccaaatttag cagataaaag
    19081 agctcctggc tggttagagt gtagacaccg attccctgcc aaatcacact tctctctcca
    19141 taaagtccaa ccccatactc gggagaaaag agatagacca tcaaaacgtt taaaacggca
    19201 aataccgtcg caaaaacggc tacaaaggaa acatctttaa agcgaatttc tgataaatag
    19261 aggagaaaga ctgaaaagat ggcaatcagc aagagcattc tggtatcata gctaatcatg
    19321 gccgccaatg ataccagaat gaaaaagaga agtttcccag ctcctgacaa gcgatgaatc
    19381 acagtatctc tatgctggta accgattaat ttagcttgca tccctctctc ctttctttgt
    19441 aaaatgccgt taaatccagt ggatccacat ctagtttctt agccaagtta aagatggagg
    19501 tttcttttag attggctttt actaacagct caggatcgct caacagactg gctggaacag
    19561 tatcggcaat caattctcca tccaccatga caaggacccg gtctgaataa tccagcatca
    19621 attgcatatc atgggtaatc atgacaatgg tatgcccttt ttgatgtaac tcttcgagaa
    19681 attccataat ctcagtatag ttcttctgat cttgacctgc agtcggttca tctaggagaa
    19741 taatttcagc tcctaagacc aaaattgaag caatggtgac acgttttttc tgaccaaatg
    19801 acagggcaga aataggccaa ttacggaatt cataaagtcc acagattttc aaggtttcat
    19861 agactctcgt ttcaatttcc ttctcatcca cacctcgcaa acggagccct agagccacct
    19921 catcaaaaat catattggtt gaaatcattt gattaggatt ttgtagcaca tagcctactc
    19981 gttccgcccg ctctgcaaca gaatcgcctt ttatatcctg tttttcccaa agatagcgtc
    20041 cttccgtctg aataaagcta cttatagcct tggctagagt tgatttccct gctccatttt
    20101 ttccgacaat agcaatcttt tcaccctttt taatatctaa atgtagggat tttaaaatcg
    20161 gtctatcatc ataagagaaa gacacgtcct ctaatttaaa gagtgactgc aattctggag
    20221 tttcttttgc cagttcattc tgcagctgaa cctgaccttt tgagatagac atgttatcca
    20281 gattcgctaa ctgttcttcc ttgactaagt ccacacctaa ttgacggaga gtcgttagat
    20341 aaaggggttc tcgaattcca ttttgagtca ataaatcagt cgcaagcaac tggtcagggc
    20401 tcccattaaa aaggatacga ccatcgctta tcaagacaat ccgatccaca gggcgatgca
    20461 gaacatcctc caaacggtgc tcgataatca gagtcgtcgt cccctcttcc ttatgaatct
    20521 ggtcaatcaa ttcgataata tcctgacctg acttgggatc tagattggcg agtggctcat
    20581 caaacaagag aatcggactt tcatcaatca agacaccagc cagactgact cgctgctttt
    20641 gtccacctga caaatcctga ggacgctgat ccagtaaagg aagaaggtcc agcttttcag
    20701 cccatttata aacacgacct ttcatctcat ctagggctgt cacatcattt tccagagcaa
    20761 acgccaaatc ttctgccaca gacaagccaa taaactgccc atctgtatcc tgcaaaactg
    20821 tgctaaccag atgagactta tcatagatgc tcatatcaaa ggctacttga ccctttatca
    20881 aaaattctcc atatgtctga cccttgtaaa tattgggaat aatcccattc aaacactgac
    20941 ccaaggtaga tttacctgac ccagatggtc caacaattaa gactttctct cccttgtaaa
    21001 tggtcaagtc tatcccttgc aaggtcggtt cttgttgtgt ttcataccgg aaagagaaat
    21061 ccttccactc aattatagct tctttcatct tactctcttc attcgcttct tagacttcta
    21121 ttttatcata aatcaagccc ttcttgcagt ctcttctctt aaaatcttag cgccaaaaag
    21181 attcctatcc tagcttactt gcctaactaa tctataaaca tcgaaaaaga ctagttgccc
    21241 agccttcccc atcattttat actcttcgaa aatctcttca aaccacgtca gcttcgcctt
    21301 gccgtaggta tggttactga cttcgtcagt ttcatctaca acctcaaaac catgttttga
    21361 gctgacttcg tcagttctat ccacaatctc aaaacactgt tttgagcaac ctgcggctaa
    21421 cttcctagtt tgctctttga ttttcattga gtattagtcc tttttcaaac ttcctgcacg
    21481 agtttgggtt cctgcatagg caagtaagag aagagttcct gcaatagcta cagatacacc
    21541 attggcaatt cccgcaacaa tcccttgtgc aaatactttt tctgccgctt cttgataaat
    21601 cacaacatct ccaagtggtg ccaagacacc ccaaacaagg gcatttgcaa gtagttgaat
    21661 gaggttaaaa ataagaatat ctttccagtc aaaaacacca ttgatcactc gaacgtactt
    21721 tctaaaaagt cccacaacta gaccaaagag tccgctagcg ataatccaag tccaccatag
    21781 accataacca gcaagagagt ccttgattgc atgaccaatc aacccgacaa gcaaaccgat
    21841 aatcggtcca aaaataatag aaagtagcgc ttgtaccgca tactgaagct ggatgcttgt
    21901 atttggaaca ggggttggaa tgttgatcat cccgatgacg acaaagaggg cagcgccaat
    21961 tccgacagca acaacttgtt taattgtaaa tttgatttcc atactattct cctattttat
    22021 ccttctattt tctttatttc aatggtccaa gatgaaccga cacctacatt ataggccttg
    22081 gcaaaggaac cttggttgat agccaaacct aaacgataga gagagttgat gtaaaggatg
    22141 ggttgcccaa ttctcacatc tgcaaatgat ttgccataga caacctgatt ttgatagacc
    22201 agcatatcag catgatagat ggtcacttca aaacgatcac caaattctgg ttccagcttg
    22261 taaaattctt cccgtgtgat agaggtccaa agcgaaccga aacgcacatc cagaatatca
    22321 atggctccct tcaccagatg atcttctatg atggtcgcta cgactggaag ctctacaatc
    22381 tgttccacac tgagctctgg ccctacttcc tcaaaagtaa tgtgaccact ggccagttta
    22441 gcaccagtat aggcatagac atcacgaccg tggaaggtat aagaatgctc tgtgttttga
    22501 cgcctattgg ccacctcaga aatctcacga atggctacaa tgccaacgtg tttcttgata
    22561 aaggaaagcg tcccattatc tggcgtgaca atgtattgat tttttgcagt cttggcaact
    22621 acactcttac gtttcgaacc gacacctgga tcgacaaccg atacaaacgt cgttccctca
    22681 ggccagtaat ccaccgtctg aaagagacga tagctcccct caaaaatatt ataaggcgtg
    22741 atatcgtgcg tcaagtgatg gatttttaag gttggagact cttctaaagc cactccaatc
    22801 atagccgaca ccgcaccatc aaccagacca aagtctgatt gtaataccag taaattattg
    22861 ttcattttat ctccttgtat atcctttatc ataccatatt tcagagaaag tcgctaatag
    22921 ctatttcaat tggttagggt atagttttac cttatagcga attttcacta aaagctcttg
    22981 ttattagcta gtaggtgcat tttttcttga aaaagggtat gatagttaca tcatgaaaaa
    23041 gtaggtttta ttatgaaaat tatccttgtc ggagggggaa aagttggttt tgccctctgt
    23101 cgctccttgg ttgcagaaaa gcatgatgtt ttgctgattg agcaagacga agctgttctc
    23161 aatcatattg tcagtcgctt tgatatcatt ggtatccttg gtaacggggc cgattttgcc
    23221 attcttgagc aagccagcgt ccaagattgt gatatcttta tcgccctgac tgagcacgat
    23281 gaagtcaaca tgattgcagc agttctagcc aagaaaatgg gagctaaaga aactatcgtt
    23341 cgggtgcgga accctgaata ttctaactct tatttcaagg aaaagaatat tctcggtttt
    23401 tctcttatcg ttaatcctga gctcttggct gcccgcgcta tcgcgaatat cattgacttc
    23461 cccaacgccc tgtctgtcga acgctttgct ggtggacgcg ttagcctcat ggaatttgtc
    23521 gtcaagtcca ccagcggtct ttgccaaatg cccatttctg attttcgtaa aaaatttggt
    23581 aatgtcattg tctgtgcgat agagagggat catcaaatta tcattccaag cggtgacatg
    23641 actgtacagg ataaagatag aatctttgtc actggtaacc gtgtcgatat gatactcttc
    23701 cataattatt ttaaatcacg cgccgtgaag agccttctca tcgttggagc aggtagaatt
    23761 gcctattatc tacttggtat tctcaaagat agtcgtatcg atacaaaagt cattgaaatc
    23821 aatcctgaaa tcgccagctt ctttagcgag aaattcccaa atctctacat cgttcaagga
    23881 gatggaaccg caaaagatat cctgctggaa gaaagtgctc aacactatga tgccgttgcg
    23941 actctaacag gagtcgatga ggaaaatctg attacatcta tgttccttga cagggtaggt
    24001 gtacagaaaa atattactaa ggtcaatcgt accagtctcc tcgagattat caatgcgcct
    24061 gatttttcaa gtatcatcac acctaaaagc atcgctgtag atacgattat gcactttatt
    24121 cgtggtcgag ttaatgccca gtattcagac cttcaagcca tgcaccatct agccaatggc
    24181 caaatcgaaa ccctgcaatt ccatatcaag gaagccaata aaatgactgc caaacctctt
    24241 tctcaactga aattgaaaaa aggggttctt attgcagcca ttattcgaaa gggcaagact
    24301 attttcccaa ctggggagga tatgttggaa gttggagaca agctcctagt aacaaccttg
    24361 ttgccaaaca tcaccaagat ttatgacttg attgcgaggt aagaaatgaa taaaagtatg
    24421 attcgttacc tcctttcaaa gttacttttg attgaagctg ttctcctctt ggttcctgtg
    24481 gctatcgctg tctattaccg tgaatcgagc caagtcttta cagccctctt ttcgaccata
    24541 gggattctcg tattgttagg cggttcaggg agtttacaga agccaaaaaa tcaacggatt
    24601 tatgccaagg agggagtctt gatcgttgcc ctctgttgga tcctttggtc tttctttggt
    24661 ggtctcccct ttgtcttttc tgggcaaatc cctagcctta ttgatgcctt ttttgaaatt
    24721 agttctgggt ttacaactac tggagcaagt attttgaacg acatttcggt tctcagccgt
    24781 tccctcctct tctggcgaag ttttacccac ttgattggag ggatgggagt gcttgttttt
    24841 gcacttgcta ttatggacaa tgccaaaaat agtcacctag aagtaatgaa ggctgaggtt
    24901 cctggtcctg tctttggtaa ggttgtatcc aaattaaaaa acactgccca gatcctctat
    24961 ctcctttaca tagccctctt ttccctcttt gtcatcatct attatctagc cggtatgcct
    25021 ctctacgata gttttgtcat tgctatgggg acagcgggaa ctggaggatt taccgtctat
    25081 aacgatggaa ttgcccacta tggtagctca ctgattacct atctggttag tataggagtt
    25141 ttggtttttg gggtaaattt caatctctac tactacctta tgctccgtcg ggttaaggcc
    25201 ttctttggag atgaagagct tcgggcttac ttggtcatcg tgctggtttc tacaggcttg
    25261 attagcctca atactctcta cctctaccca gggttttcaa agagctttga aatgaccttc
    25321 ttccaggttt ccaacatcat tacaacaact ggttttggat atggagatat taccaactgg
    25381 cccctcttct cccagtttat cctccttttc ctcatggcaa tcggtggctc tgctggatca
    25441 actgcaggtg gactcaagat tattcgaggc ctcatccttt caaaaattgc caaaaaccaa
    25501 attttgtcaa tcctatcgcc ccaccgtgtt ttgaccctcc atgttaataa aatggtgatt
    25561 gacaaagata cccagcataa gattctcaag tactttgtca tctatgctat gattttgcta
    25621 gcgcttatct ttattgtcag cctagatagc aatgattttc tggtcgtgac cagcgctgtc
    25681 tttagctgtt tcaataatat cgggcctatt ctaggaacca cttctagttt ctcaatcttt
    25741 agtcccatct caaaaattct cctctccttt gcaatgattg cagggcgctt ggagatttac
    25801 ccaatcctcc ttctctttat gaaaagaact tggtctaaga gataaaatac aaaatccctc
    25861 tttttacaat tgtgaaagag ggatttttat attaaaaagg gggaacgttt ttaaatccaa
    25921 gcctgtctgt aagcaaagtt cttactcaaa taaaagatgg aaaaacttac tacacagctt
    25981 ggtaatgctc caatacaaat tgtaaggctt tacttggatt ctttgttaaa tgaatatatt
    26041 ccattcctct ggtagcaact atctgaatac ctaatctttc tgcatccacc ttcatctctc
    26101 tataataact ccgaacttgt ggagccagaa taattaaatc ataaacaccc ataatatcat
    26161 aatgagctcc gtacgctcct gaattcgcaa tcactctaac ttctgctaat tgagcgccct
    26221 cgttaattgc attggctaat tacgcacttg ttccagaccc tgcacaaaga accagtactt
    26281 tcaactcctt actttttatt acatctatct caccaggcat tatttcactt gtatcctcct
    26341 ctaaaataat atcatttgag cttgcaatat ctacaacctg tagtagatat ctcgtattta
    26401 gacaatatga aaacaagacg acttccatat aggaaaccgc cttctctcta tgttgagtga
    26461 tttatattaa aataactttt cttctagctg cattttatta ttataaaaac attcatcata
    26521 accctgaact taaataacga tttttattca agatacatac tcctagaata aactttatat
    26581 gaaattctca tttttgtttt tacaattctc cttagttaaa tcttgtttaa tatatgtttt
    26641 acatatagta tttagcgcca catagtactg aactctctcc aaaaacggtt attcctcttt
    26701 gaatagggcg ttatcacaag aaaagcatct ccacgtttca acttcatatg gctcaaaaac
    26761 aatcaattga tgctaaaacc tgtacctaga tgtttcggtt cataaaacca tgaaactgta
    26821 aaagtgaatg aaattgatag cgatagtcaa atcaagaggc atcataactc taaaaagtca
    26881 caatatataa gttcatcctc ggaaaaatat cattctaatt gttgaaatgc ctacatgaaa
    26941 agaaacgtca aatgctcatg aaacaacgaa tacaggtatc aaaactatga caaaacaagt
    27001 ccctaaattt actaaagaca ctgctcaact ttacacctgt aagtggttgt tgtataataa
    27061 agttacaaag atgtacgacc acactgttgt aaatcatagt gttcgcgaat atattactga
    27121 tagcatttct acaaatacaa ttgaagagac tggatagact ttaaacgaaa tatgtcagtg
    27181 ttttggcatt cctagccttc atatcattta aagaattcta tagacaaaat ttttcccaat
    27241 acagacactc gtaacaactg cttcattttt ctaccaacat atttaggaac aggataagat
    27301 acaagaggat taatccatag atcagttcta taccaatcta agacaaataa gctaaaaaaa
    27361 cgattgataa taagcaaata gattccaaat tttctctatc tgctcatttt aataaacaat
    27421 actagtgtaa ctatccttcc agtcagaaac ttgtcaaatc acaccgaaaa ctcttctaaa
    27481 atttatctcg ttaggcaatc aagcaaaaac tcgacgatag tacaaacatt atcatacaga
    27541 attgactgcc taaattatat actttagtaa ggttttcgga taagaaaaaa ggttcatttt
    27601 acatttctaa acattctttt ctaagatgaa aaacagaatt tttcgattgt gatttaaagc
    27661 aacaagaaga ttttcagtat catcctatag atacgagcta attaagaaaa actacatttt
    27721 tgaatataaa ctacaataat ataaactaaa ttttatagga ggaagacaat ggattggtac
    27781 gattatatga tacaggcatc caaacaatca caattcaacg caagccattg gtttcgctat
    27841 ttgcgaaaag ttatttttga agactattct tatttaacaa accaagatgt agaaaagttg
    27901 ctagactcca aagaactaac ccgttttcaa aaaattagct tgaagtatgc ctttcaagag
    27961 catactccaa ctcataaata tgtgatttca ttaaataaac ctgctaagtt aaccaatgtt
    28021 caaaaattga tggagaaata caaacatgga taaaatgaaa ccggtcttcc aagccctaaa
    28081 taagaaatta attcaggaaa atctgacttt aacaattatg gttatgtctt agaatatcat
    28141 ggtttacgta ccacacaaga tgttgatgct ttttatgacc aaaatcagaa aatcaatgaa
    28201 attattgctc gtgtaggaaa acagtttaat cttaacatcc acgaagagtt atggctcaac
    28261 aatcatgttg caaagcaaat atgaacaaac aacctccctt aagtttatgc gaatccttgt
    28321 actcatttga gaatctgacc gttttagtag tccctattga gtatgtgtta ggaatgaaga
    28381 tgatgagtat cagagagcag gatttgcaag atattggtgc aattattaaa tacaaaaact
    28441 ttcattcacc attcgatacc tttaaatatt taaaagacat gggatttgat actattgacc
    28501 tctctgttct attagaaggt tttagttatg cttatggtat ggattggtta gaaaaatttt
    28561 ttaaagaaaa tcaagataaa cttcgcgaat tttattaaca gtatcaaaca agatgattgt
    28621 caattagcca gtcatcttat ttttatgatg aaatgaaata aaatcggaac aaatcactta
    28681 gagaattcca gtcaatttct agcactgttt gagccgtttc agcggactat agtaaaatga
    28741 aataagaata ggacaaatcg atcaggacag tcaaatcgat ttctaagaat gttttagaag
    28801 taaaggtgta ctattctagt ttcaatctat tatatttcag attcggtcca ctattccttc
    28861 ccaaaaacat tgcatattgc gccgaaggaa ttgctcatac tatcccaagc acaaaaccct
    28921 cagtcgtttg accgagggtt ccttttcatt attcaaaaac ttgattagct caatgcatcc
    28981 aaggcatcat ccattgaaag aacttcgtgg aagacacgtt gtgtcaattc agttttttgt
    29041 tctggagtga ggtatttagt atttacacag tatccagaga tacgtacgat aacgtcttca
    29101 cctgacataa ttttttcgta aacatcgttc aagtccataa cgttcaagtt aacgtgttgt
    29161 ccaccgtttt cgaagtaacc atcaaggatt gttaccaagt tatcaacttg ttcatcacga
    29221 gtcttaccaa gagcgcgagg tgatacttgt gtagtcaatg agataccgtc agctgcataa
    29281 ctaaagtcaa ggctagaaag tgagttcaag ttttgcaacc aaccaccttt agctttgtta
    29341 gatgggttag cacctggtga gaagaattca agtttagaca agttcacaga accatcttcg
    29401 ttgaggtata cacctttgtg aactggtgag ttaccagttt gtttagagta agcaacgtta
    29461 gatgtgattg tcaaaagtga tactgtagct tctgcgtctt tgtatagttt gtggctacgt
    29521 agacgagttg tgtaagcttc gatcaaccat tctgccaatt cgtttgaacg tgggtcatct
    29581 tcaccccagc gtgggtagtc accgattgtt tcgtaatcgt agatgtagcc atcttcgtca
    29641 cggattggtt taactgtagc gtatttgata gctgacaatg tatcaacagt gttagcaaat
    29701 ccacagatac cgaatcccat gttggcacgt tgtttagttg gcaagaaggc catttgaaca
    29761 gcttcgtagt tgtacctatc agtcatgtag tggatgatgt tcaaggcatc tacgtaagtg
    29821 tcagtcaacc agtcaagaga tttttcaaag ttcgctttaa ctgattcgaa ttcaagaact
    29881 tcgtcacgga ttggttcgat atcaaatact ttgtagtctt tgtgaacatc gtcgtaacca
    29941 ccattcaaac cagtaagaag ggctttaaga acgtttacac gagcaccgaa gtactggatg
    30001 ttgtggcgtt gttcttcatt ttctggatca agtggagaca cacagcatga gatacagctc
    30061 atttcaccat atccgtcttt agccattgtt gttacacctt cgtattggat agaagagtgt
    30121 ttgtggctca tgtgcataca gtagcgacgg aagttgtatg gcaatttgtc agtccaaaga
    30181 actgtcaagt ttggttctgg tgagttaccg atgttgtcaa gagtgttcaa gaaacggtag
    30241 tccatcttag taacacggtg acgaccgtcg ttacccatac cagccataga agttgtgata
    30301 aaggttgggt cacctgagta caattggtca taagcttttg tacgagcaaa tttaactgta
    30361 cgaagtttca taacgaaatc atcaacgaat tcttggattt ctgattcagt aaatgtacca
    30421 cgagcaaggt cacgttctgc aaagatgtcc aatacgattg gtacacgacc tagagatgta
    30481 gcagcaccgt taatcacacg gcagacagcc atgaaagcaa tgttaaccca tcggattgct
    30541 tctttcacgt tcatcgctgg tttgcgaaca tcaaccccgt aaaggtcacc caggcgaaca
    30601 acttgttgca atgcttggta ttgaaggttt acttcttcac gaagacggat tgtttcttca
    30661 tcgatttctt tgattgcatt ccagtcattt actttttctt gcatcaagta gtctgcaccg
    30721 taaagagcaa gacgtgcgta aacaccgatg atacgtccgc gtgagtatgc atctggaaga
    30781 ccagttacag tgtgtgcgtg acgagcgcga cgaatatttg aagtgtaggc acggaaaata
    30841 ccgtcgttaa ctgttgttac atatttagtg aagatttcgt gaacagctgg gtctggttcg
    30901 tatccatttt ctttcaaagt agtttcagcc atacggatac caccttttgg catgaagttc
    30961 aatttgaaga gttcatcgtt ttggataccg aagataactt cattttcttt gtcgataaat
    31021 ccagcaggga tatcagcgat agatgttgga cgagtgtcca ttgggaaacg agtttcttcg
    31081 tagtgtgctt tagtttcttc tacaattttc ttgatgtgaa gtgaacgctc tgttggtcct
    31141 gcaaggaagc tttcgtctcc atcataaggt gtgtagttag cttgtacaaa gcgtgataca
    31201 cttgcttttt ctttccaatc tacgcctttg aagccttccc aagctttgtc aaaaatatct
    31261 tgtgcttcaa caactgtctt aacaaccata ttaatgtcct cttttttcta gtaacagtca
    31321 tctgttacat tcatgagaca agtataccat atagtaaccg attttaacaa gtgaaaaatc
    31381 cctattttta cactttcttt tctaaaacag tctatatttt atttcaaact gtattatatt
    31441 tttgaaaaaa taaagtcctt ttttcttttt ttcagaaaaa agggtataat aaaagaaaat
    31501 aagcagtaac actcaatgga aatcgaaaag caaactagga agctagccgc aggctgctca
    31561 aagcactgct ttgaggttgt aaatagaact gacgaagtca gctcaaaata ctgttttgag
    31621 gttgcagatg gaagctgacg tggtttgaag agattttcga aaagtataaa aaggtgctag
    31681 gcatgttgat ttttcctttg ctaaatgatt tgtcaagaaa aatcatccat attgacatgg
    31741 atgccttttt tgctgcggtg gaaatcaagg ataatcctaa actcagagga aaacctgtca
    31801 ttattggaag cgaccctcgg caaacaggtg gacggggagt cgtttctacc tgtagctatg
    31861 aggctcgagc ttttggtgtc cattctgcca tgagttccaa ggaagcttat gaacgttgtc
    31921 cccaggccgt ctttatctca ggaaattatg agaaatacaa agctgtggga ctccagattc
    31981 gagctatttt taagcgctat acagatttga ttgaacccat gagcattgac gaagcctatt
    32041 tggatgtgac agaaaataaa ctcggtatca agtcagcggt caaaattgct cgcctcattc
    32101 aaaaagatat ctggcaagaa ctccatctaa ctgcttctgc cggtatttcc tataacaagt
    32161 tcttagctaa aatggcgagt gattatcaaa aaccacatgg tttgacagtg attctacctg
    32221 aacaggctga ggattttctc aaacaaatgg atatttccaa atttcatgga gtaggaaaaa
    32281 agacagtaga acgtcttcat caaatgggcg tttttactgg tgctgattta cttgaagttc
    32341 ctgaggtaac cctaatagac cgttttggta gactaggcta tgatctgtat cgaaaggctc
    32401 gtggcattca caactctcca gtcaaatcca atcgcatccg taaatcaatc ggcaaggaga
    32461 aaacctacgg gaagattctc cttgctgagg aagatatcaa aaaagagctg actcttctat
    32521 cagaaaaagt cgctctcaat ctacatcaac aagaaaaagc tggaaaaatt gtcattttga
    32581 aaatccgcta cgaggacttt tcaactctta ccaaacgaaa aagtcttgct caaaaaacac
    32641 aagatgctag tcagataagc caaatagccc tgcaactcta tgaagaatta agcaagaaag
    32701 aaagaggtgt ccgcctattg gggattacca tgactggatt ttaaagctca atgaaaatca
    32761 aagagcaaac taggaggcta gccgcaggct gctcaaaaca ctgttttgag gttgcagata
    32821 aagctgacgc ggtttgaaga gattttcgaa gagtataaaa ccttgaagag ttgccccttc
    32881 aaggtttttc ttatacaaat aaacggacaa aactgtaaag tagcaaaaca ctaccaagca
    32941 cgatacggta tttaccaaaa agggtgaagt cgtgtttttt cacatagctg gtcaagaagc
    33001 gaatagccac catgctgacc gcaaaagcta ctcccatcgc gaccaagagc aaaaacaatt
    33061 gcccaaagct caagagttct ccggctttca caaatttgaa aatctttaag gcactagctc
    33121 caaacataac aggaatccca agatagaagg taaattctgt cacaactgaa cgactggttc
    33181 catttaacaa accaccgaca atcgttgcac ctgaacggct agtccctggt aaaagagcaa
    33241 gaacttggaa gagtccgata tagaaagcgg tcgtataagg aagcttgtcc aactctgtta
    33301 cacttggctc gatagcacgc gctttattgc gcttttccaa atagatgaag gcaaccccgt
    33361 agataatcaa catgagagca actgaaacca tgttatggaa gtgggtatca aaccaatcat
    33421 caaatttaaa gacaccaagt aaaggtaaag tagcaatcaa gaccttcaac catagtctcc
    33481 aagtcttacg aacttcctgt ttgtccttag tcggtttaaa aggattgagc ttgttaaaat
    33541 aaatcaccat aactgctaaa atagcaccaa gctgaatcac gacattaaac atggacataa
    33601 aggcttcatt ttgattttgg tattggatga attcctctgc taaaatcaag tgacctgtac
    33661 tggaaatcgg caaccattcc gtaattcctt caacaatccc gaagaagata gattttaaaa
    33721 tttcaataag atacatagat tactccctat attataatta gcgcagttag ctactgccaa
    33781 agcccaagtg gtatacttgg aataagccac tgtggattag ttcatttcct ccaatcgctt
    33841 gacgatttcc gaaaggatct aaccacagct tttgtttcaa ttggtaatga gtttgataac
    33901 gcatagacgt tttatctcgt ctaaggtaac aaggcaaaaa gttcgtggat aaccaagtag
    33961 acttgaaata ctatatctgg aggtactccc atgctttatg tgggcattga tatcgctaaa
    34021 aataaacacg atgttacagc cttgaatgtt ccaggaaaaa ctgttcttaa accactcact
    34081 ttttcaaata ataaagctgg ttttgaactc ttagatctgt ctcttcgaca gctcaaccaa
    34141 gactgtctca tcgctcttaa acttctttct gatcccaatc gtgaacaatt tcaacacgat
    34201 aatcggcaag tagacctaaa aatactggct agacatattc atcgtctcaa gaaaaaacag
    34261 tctgattgga aagtacaata cactcgttgt cttgatatca tctttcctga gttggataaa
    34321 atcgttggaa agcattcaga atatacctac caactcttga cgcgctaccc taatcctcag
    34381 aaaaggattg aggcaggatt tgataagctg atagaaatta agcgattgac cgcttctaaa
    34441 attcaggata tcctctcagt cgcacctcgt tctatcgaaa caacatctcc tgctcgtgaa
    34501 ttcgaaatca tcgaaatcat caaacattac aagaggctca ttgacaaggc ggaaacatgt
    34561 gtcaatgact tgatggctga gttcaactca gtcatcacga cggttactgg gattgggggt
    34621 cgtttagggg cggtcatttt agccgagatt cgaaatattc atgcctttga taatcctgct
    34681 caattacaag ctttcgctgg actggattct tctatttatc agtcaggtca gattgattta
    34741 gctggaagaa tgatcaaacg gggttcccct catctgcggt gg
//
LOCUS       DBEJAQ010000022        34127 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.022, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000022 DBEJAQ010000000
VERSION     DBEJAQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.022"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(443..1132)
                     /gene="rplA"
                     /locus_tag="ACL297_001088"
     CDS             complement(443..1132)
                     /gene="rplA"
                     /locus_tag="ACL297_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGR0071363.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1340..1765)
                     /gene="rplK"
                     /locus_tag="ACL297_001089"
     CDS             complement(1340..1765)
                     /gene="rplK"
                     /locus_tag="ACL297_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGR0071364.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(1888..2604)
                     /gene="ldcB"
                     /locus_tag="ACL297_001090"
     CDS             complement(1888..2604)
                     /gene="ldcB"
                     /locus_tag="ACL297_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HGR0071365.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(2622..3083)
                     /locus_tag="ACL297_001091"
     CDS             complement(2622..3083)
                     /locus_tag="ACL297_001091"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGR0071366.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(3085..4122)
                     /locus_tag="ACL297_001092"
     CDS             complement(3085..4122)
                     /locus_tag="ACL297_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGR0071367.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            complement(4220..5545)
                     /gene="brnQ"
                     /locus_tag="ACL297_001093"
     CDS             complement(4220..5545)
                     /gene="brnQ"
                     /locus_tag="ACL297_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGR0071368.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5716..6003)
                     /locus_tag="ACL297_001094"
                     /pseudo
     CDS             complement(5716..6003)
                     /locus_tag="ACL297_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(6163..6741)
                     /locus_tag="ACL297_001095"
     CDS             complement(6163..6741)
                     /locus_tag="ACL297_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGR0071369.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6795..8195)
                     /gene="pepV"
                     /locus_tag="ACL297_001096"
     CDS             complement(6795..8195)
                     /gene="pepV"
                     /locus_tag="ACL297_001096"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGR0071370.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8208..8813)
                     /locus_tag="ACL297_001097"
     CDS             complement(8208..8813)
                     /locus_tag="ACL297_001097"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGR0071371.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            8984..9784
                     /locus_tag="ACL297_001098"
     CDS             8984..9784
                     /locus_tag="ACL297_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0071372.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9968..10801)
                     /locus_tag="ACL297_001099"
                     /pseudo
     CDS             complement(9968..10801)
                     /locus_tag="ACL297_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(10791..12638)
                     /gene="uvrC"
                     /locus_tag="ACL297_001100"
     CDS             complement(10791..12638)
                     /gene="uvrC"
                     /locus_tag="ACL297_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGR0071373.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGSIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12678..13379)
                     /locus_tag="ACL297_001101"
     CDS             complement(12678..13379)
                     /locus_tag="ACL297_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HGR0071374.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLAGQ
                     FLSVTVRLNHYYLSNPHYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13458..14690)
                     /gene="murN"
                     /locus_tag="ACL297_001102"
     CDS             complement(13458..14690)
                     /gene="murN"
                     /locus_tag="ACL297_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HGR0071375.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEYFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14695..15915)
                     /gene="murM"
                     /locus_tag="ACL297_001103"
     CDS             complement(14695..15915)
                     /gene="murM"
                     /locus_tag="ACL297_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HGR0071376.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16027..16806)
                     /locus_tag="ACL297_001104"
     CDS             complement(16027..16806)
                     /locus_tag="ACL297_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGR0071377.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16875..18536)
                     /locus_tag="ACL297_001105"
     CDS             complement(16875..18536)
                     /locus_tag="ACL297_001105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGR0071378.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18711..20933)
                     /gene="recJ"
                     /locus_tag="ACL297_001106"
     CDS             complement(18711..20933)
                     /gene="recJ"
                     /locus_tag="ACL297_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGR0071379.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GGASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21210..21968
                     /locus_tag="ACL297_001107"
     CDS             21210..21968
                     /locus_tag="ACL297_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGR0071380.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            22008..22772
                     /locus_tag="ACL297_001108"
     CDS             22008..22772
                     /locus_tag="ACL297_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGR0071381.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQGDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     EFNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22785..23462
                     /locus_tag="ACL297_001109"
     CDS             22785..23462
                     /locus_tag="ACL297_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGR0071382.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23472..24131
                     /locus_tag="ACL297_001110"
     CDS             23472..24131
                     /locus_tag="ACL297_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGR0071383.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNSVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24427..25617
                     /locus_tag="ACL297_001111"
     CDS             24427..25617
                     /locus_tag="ACL297_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HGR0071384.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25938..26819)
                     /locus_tag="ACL297_001112"
     CDS             complement(25938..26819)
                     /locus_tag="ACL297_001112"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGR0071385.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26960..28288)
                     /gene="vncS"
                     /locus_tag="ACL297_001113"
     CDS             complement(26960..28288)
                     /gene="vncS"
                     /locus_tag="ACL297_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HGR0071386.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(28285..28941)
                     /gene="vncR"
                     /locus_tag="ACL297_001114"
     CDS             complement(28285..28941)
                     /gene="vncR"
                     /locus_tag="ACL297_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HGR0071387.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28990..29073)
                     /gene="pep27"
                     /locus_tag="ACL297_001115"
     CDS             complement(28990..29073)
                     /gene="pep27"
                     /locus_tag="ACL297_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HGR0071388.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(29253..30632)
                     /gene="vex3"
                     /locus_tag="ACL297_001116"
     CDS             complement(29253..30632)
                     /gene="vex3"
                     /locus_tag="ACL297_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HGR0071389.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30684..31331)
                     /gene="vex2"
                     /locus_tag="ACL297_001117"
     CDS             complement(30684..31331)
                     /gene="vex2"
                     /locus_tag="ACL297_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HGR0071390.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31344..32621)
                     /locus_tag="ACL297_001118"
     CDS             complement(31344..32621)
                     /locus_tag="ACL297_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071391.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGNSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(32978..33148)
                     /locus_tag="ACL297_001119"
                     /pseudo
     CDS             complement(32978..33148)
                     /locus_tag="ACL297_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(33291..33890)
                     /locus_tag="ACL297_001120"
     CDS             complement(33291..33890)
                     /locus_tag="ACL297_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071392.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYTTIDKTSDATALLKLKLNNVDNQPATEVLSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNYP
                     VGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
BASE COUNT         9644 a         7793 c         6310 g        10380 t
ORIGIN      
        1 aaacacgaaa ccagtccacg tttttcaagg actggttttg atatagcacg tttaagtacc
       61 gacttctgag ctactatagt agattgaaac tagaatagta cacctctact tctaaaacat
      121 tgttagaaat cgatttgact gtcctgaacg attcgtccta ttcttatttc attttattat
      181 aattgatagt ggtcgcccca gccagatacc ttatccgcta tataggcctt attatcacgt
      241 tcctgttcgt agagccacga tttcgctatc ccttcctctg acgattctta tttccagcgt
      301 cagacttggg aatcgctatt gggttcaccg gtagtaggtg cccaccgagg acttacacct
      361 cagatatacg acatgcccgt cgtacatcaa aaaaagttga gacatagtgt cttcaacttt
      421 tttacattac tttaactact gattaaagtg agtttacgtc aactttgata ccaacacctt
      481 gagtagttgt gatagtcaag tttgttacgt aagttccttt agctgtagct ggttttactt
      541 tttggattgt ttcgttgaaa gctttaaagt tttcaaccaa tttttcagct tcaaatgata
      601 ctttaccgat gattgcttga acgttacctg cacggtcagc acggtaagtg attttaccac
      661 ctttagactc ttcaactgct ttagcaacat ccattgttac agtaccagtt ttagggtttg
      721 gcatcaagtt acgtggtcca aggacacgtc caagacgtcc aacaagagcc atcatatcag
      781 gtgtagcgat aactacgtcg aagtccaacc aaccgtcgtt gattttagca acaaggtcat
      841 cttcaccaac aaagtctgca ccagcagctt ttgcttcttc agcttttgca ccacgtgcga
      901 aaacaagaac acgtgaagtt ttaccagtac cgtttggcaa taccattgct ccacggattt
      961 gttggtcagc ttttttaacg tcgatgttca agttgtaagc aacttctaca gttgcatcaa
     1021 attttgcaaa gttagtttct tttgcaagtg ctacagcttc ttctacactg tatgcttttg
     1081 tgctgtcgat tttctcaaga gcagcacgaa gttgtttgct ttttttagcc attttctatt
     1141 ctccttgtaa gtggttcaat cgattttcat ctcccacgtc acttctcgaa atgatgaagt
     1201 cttgcgggtt ttcagtctac tatttttctc ttcctacggc cttaagctcc cttgctgtat
     1261 ccaagtacaa gcctcagtca cttgcctagt atgaaaagca actaatagac tgctgtgaga
     1321 ttttctactg tgttattgat tagtcaacaa cagtgaatcc catagaacga gcagtacctt
     1381 cgatcatacg cattgcagac tctacgtttg ctgcgttcaa atctggcatc ttagtttctg
     1441 caatttcttg tacttgtgca cgagtaactg tagcaacttt agttttatta ggtgtacctg
     1501 atcctttttc aacacctgca gcttttttca aaagaacagc agctggtggt gttttcgtaa
     1561 cgaaagtaaa tgatttatct tcgtaaactg agataacaac tggaatgatc ataccagctt
     1621 ggtcagctgt acgagcgttg aactcttttg tgaatcccat gatgttgata ccagcttgac
     1681 caagagcagg tccaaccggt ggagctggtg tagctttacc agcagggatt tgcaatttta
     1741 caagtttttc gactttttta gccattttta aatcctcctt tgtggttttg gcggtaatta
     1801 gagattttta cctcccacaa gtatgcttta cacatacctt tccattatac actatttatc
     1861 taaaaatgca agagaaaact ttcattttta atcgacataa tcgccgcctt caaagccata
     1921 gtactcttcc aaactgagac cacttgcagc aatttctttg gcttcttttc caacgtagcg
     1981 gagatgccat tcttctgcca tgtagccagt ctccttttcc ttgcctttaa gatagcgaac
     2041 aacaaagcca tagtcagccg catggtccaa gagccactgg gctgcctttt cttcagtaac
     2101 taaatcccca tctgtcccaa tcaaatcaaa agcaagaccc gtctggtgtt cactgtaacc
     2161 tgggcgtgct gagtagcgat ctgctgcttc cttaccatct tgattgacat agtcttggta
     2221 gagcttagcc tgagtttcat aactcctaaa cccactgtat tggtcactga ttggatagcc
     2281 ctcagcctgc atagctgcga ttagtttgag caactctgcc ttggctgttg gattttctcc
     2341 tggattgtaa tcttttgaca aaggataacg tttattagct aggataatct catcgtattt
     2401 cccttggata ctataatagc tccctttatt gctaacttct gcctttttct gactagaagc
     2461 ttgagatttg ctacctacag ttccttccgg ttgactgctt tgctccgttt tttgagtgtt
     2521 ttcttcaact ttggtttttt cttgcgaaca agctgcaagg gtcaatgcta gtaaaccaga
     2581 taaaatgaca tatctttttt tcatttcttt cttcctctct cttaggccaa atacttctcc
     2641 aattcatgag ataagatttt aatcatctct tccagctctg gcgattgtac ttggcaatct
     2701 tctgccgcca tttcttccca gggcacccac cagactgggc cacggccatt caaaccgtga
     2761 ctcttcatat cacctgctct tcggggaaaa agatgccaat gagcgtgggc atcgccattt
     2821 cctaggagtt caatattcat cttttcagcc tcaaatgctt tggcgactgc ttcttggact
     2881 aaactcattt cctctaaaaa gcgaagtttt acagaagttt ccatatgatg gagttctgtg
     2941 acatgatctt ttgctaaaaa taaggtatag cccttaaagt attggtagtc accaataaca
     3001 acatagcctg tttctagttc ttttacaaag tagggatttt gcccagcttt aatgagttcg
     3061 attctctgac agattaagca cattctatga taccgcctcg ctcttaagat aagcatcaac
     3121 catacgctca gtcgctatta ctgaatcgat gtgggtgcgc tcataagagt gactggactc
     3181 aataccagct ccaagaagtg catgcttgac ctctgcacct gctgacatcg ctgctgaagc
     3241 gtccgatccg taaaatggat agatgtcgag cttaaatgga atatcttgct ctttagccaa
     3301 ggcaaccaag tgttgacgga agtcatagtg ataaggacct gaagcatcct tgacacagat
     3361 agatactgtg tactcatctg tctgctggtc atctcccata gctcccatat ccacagccag
     3421 atattctact acctgagcag gaatgttaga attagcacca tgtcctactt cttcaaaaac
     3481 tgaaaaagca aaatgagttg ttactggcaa ttcaatcttc tctttcttat aaatacgaag
     3541 caggttgagc aaaatcgctg cgctgacctt atcatccaaa tgacgagact tgataaaacc
     3601 tgtatcagtc acaacagttc gtgtatcgaa gctgataaaa tcaccgacct caatcccaag
     3661 agcacgagtt tctttttcat tgcttacttt tacatccaaa cgcacttcca tattgtcctg
     3721 cgtgcgttct gcagttcctg catccttgta gacatggcaa gaagtttggt ggatgaggat
     3781 ggttcctgat accttttgac ctgtgctagc cacatgaacg gtacagtttt caccttcaat
     3841 catgttccaa ggaaaaccac cgatacggtc caatttaaga cgaccatctg gtttgacagc
     3901 acgaacaata gcacccagcg tatccacatg ggcagtcaca tagcgatgtt gctcatcatt
     3961 ttgacctttg atggtcacat tgacaccacc cttagctgtg cgaactggct ggtaaccaaa
     4021 accttctaga gtcttgacta aataattcga aatttcacga gtgaagcccg ttggcgatgc
     4081 gatggctgtc agttctttga tatattctac tgtttgattc atttcttcac ctctttcgct
     4141 acctattata acacatttat catcggataa aaaaagaaaa tcactcctgt agacttggct
     4201 acaaaagtga tttaagtgat tattccattt caaaaacatc gctttcttgc ttgtttggta
     4261 gaaccaatga gagcaagatt ccaacaatgg ctggcaccaa ccatgggaga gatgccttgg
     4321 caaaaggaag aacgctaaca agatttgcaa gaaactcaac cttaaacgag cttcctagta
     4381 cgcttgcaat ggcaataact gtaaccacag caattgtcaa ctgcatacct ggttttgaaa
     4441 gggcaacaaa tttgttgaca atgacaatca taacgatagc aatcgtgatt gggtacaaga
     4501 taaccagtac tggaattgag tacttgataa tcgcatcaag acccaaattg gcaatagcaa
     4561 atccaatcaa ggtaaaggct gtcgcataaa ccttgtagct gatttgtggg aagcgctcat
     4621 taaagaactc agctgttgac acaatcaaac caacagtcgt tgtgaagcag gttacggtaa
     4681 ccatagctgc aaggaagagt tgagctgttg agccaaagat ttcttgagtg gcttgtgaca
     4741 agatgtaaac acctggtgtt ccacccttca tcgcttcagc tggtactggg aaatgatttc
     4801 caagaaaacc taaaccgatg taaagagcgc tgaaggcaag ggcaacaacg ataccaacaa
     4861 cccaaatagt tgaaatgtat tctttcttac ttgaaaatcc aagttgtttc aaggtttgaa
     4921 ctgcgattac gctaaaggcc actgaagcaa gggcgtccaa ggtattgtaa ccttctagga
     4981 aacctgtacc aaaggcagaa gcttgataag cagctgaagc agcttgagga cttgttccac
     5041 catatttgat agctcccaga acgaccaaga taacaatcaa aattgcaaag actggcgtta
     5101 aaacacgtcc aatgcggtct aagatttttg atggattaag cgaaatcaaa taggctgccg
     5161 caaaatacag aaccgtaaat acaatcaagc caagtccttt atttgcatcc gacaaaaggg
     5221 ggctaatccc tacttcgtaa gctgttgtag cagtacgtgg ggtagcaaag aatggaccga
     5281 ttgacaagta aagaactgag aggtaaagag tcgcaaacca aggcgctatc ttcgttgaaa
     5341 tctcgtagat atatccttta ggatttagcg ttccaataat aagggtcaag acggcgatac
     5401 caacgcctga aaagacaaaa cctgcgatgg caggaagaaa atgttctcca gatagagcac
     5461 ctagagaagg cggaaaaatc aagttccccg caccaaaaaa tattccaaac aggagcaaac
     5521 ctgttagggt acctttttta gccataaaaa tctccttcat atttataaaa tactgaccta
     5581 gtataccacg attaggagtc tgaaaaaagc atcacgaggc aaatattaaa tgttttcaat
     5641 tttcttaaaa ataagaatta tctaaaaata aaactctcct acccagtcca caccaagcag
     5701 gagaaatgca aaattttaaa gttcttcaaa ggctaacatg ccaccctgga cattgataac
     5761 attataacct tgttctaaca ggaattggca agcacgcgcc gatctcattc cagatttgca
     5821 aataatataa tgcaagcgat ctttgtccaa ttaatcataa ctatcagcca attgactaag
     5881 cggtaggttg tgggcacatt ctaaatgaag agctgcaaac tcatccactt ctctcacatc
     5941 cactagagaa agctggtcgt tttggtaaag ctggtaaaat gcgtcaaagg ctatttcttt
     6001 cattcttttt cttctaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac
     6061 cgtgcgtatg ttaccgactt cgtcagtttt atctgcaacc tcaaagctgt actttgagca
     6121 gcctacgact agcttcctag tttgctcttt gattttcatt gactataaaa tggttttaat
     6181 tctttttttc aaatctggca ctacttctgc ctcaaaccaa ggatttttgg ccatccagat
     6241 ttgatttcgt ggtgatgggt gaactagcgg aaaataggct ggcagatagt ctttatagtg
     6301 tttcaccctc tccgttacct tcccactgat tttctcctgt aaatagtagg cttgggcata
     6361 ttgcccaatc aagagggtta actgaatatc aggcaattcc tgtaagagct gcggatgcca
     6421 tttttctgca aaacctgtac gaggcggaag atcacccgac ttgccatgtc ctggaaagta
     6481 gaaatccata ggcaaaacag caaaataacc tgaattgtaa aaggtatctt catccacacc
     6541 tagccagtcc cgcaagcggt caccactttt atctttccag taaaggcctg cttcttgagt
     6601 tttaagtccc ggagcctgac cgatgatatt gatgcgagca gtttttggcg ctgcaaagag
     6661 aggctcaatg ccacgctctg tatagctggc attctgtgaa tccgccatga tagcctgttt
     6721 gattctttcg atttgagaca tctactttcc ctccaaaaag aagtccaagc ataagatctc
     6781 agacttctat cgttttattt gatcaattcg taaatagctt cggcatagat tgctgctgct
     6841 cggaagagat cgtccaaggc gataaattca ttggcttggt gcatggtatc aattgagtct
     6901 gggaacatag caccataggc aactccgcgc tctaacaagc gaccaaaggt tccaccaccg
     6961 atgacttgtt catgaccttt aaagccagtt tgtttttcat agatattcaa caaggtttgc
     7021 acaagtggat cttccattgg cacatagtga ggcgtgtgac cgtgttcaga caggctaaca
     7081 gaaacaactg gcaagttttc aaggattgac ttgatttgtt ctggacttgt tccttttgga
     7141 tagcggatgt tgagggcaat ggtattatca gcacttgttt catcgaagcg gaagacgccg
     7201 gcattcatag aaagagcacc catcttttca tccacatgag caatcttaag attttcaccc
     7261 tcatgatcgt tcaagagaat tttacctgcg atgtcaaggt agtctttggc tggaccagca
     7321 aagccaaact gactgaggaa gagggcaagg taagttgcgc cattgacacc tgaagcaggc
     7381 atagcaccgt gggctgattt accaatgatc gtcaccttgt atttgccagc ttcttcttgg
     7441 agttctcctc taagtttgtg ttctgcaaca aaggcatcta gtttagcttg caagtcagcc
     7501 aagtcacctg aaacgactgc tgttgctgat tctggtacca tattttcacg taaaccacct
     7561 gtaaagctgt gaagacgggc aacacctgta ttttctcctg caaagtggag gtattccgtg
     7621 atatttcctt tttcaccatt gatgattgga aattcagcat ctggtgagaa accgaaatct
     7681 ggtttggcaa gtcctacgtg ctcaaagtag tagtccatgt ctgcccagcc tgattcttcg
     7741 tctgttccaa cgatgaagcg aactttctta gaagttggaa gacccaattc tttgatgatt
     7801 ttcaaaccat agtaacaagc tgttgtagga cccttatcgt ccgaagcccc gcgcgcataa
     7861 aggcgaccat ctttgatagt tggtgtgtaa gggtctgtgt cccaaccgct accagcaggc
     7921 accacatcca tatgggcaaa gattccgaga acttcttctc catcaccaaa ctcaaaatgt
     7981 cctgcatagt tatcaacatt cttagttggg tagccatcgc ggtctgcgat ttcaaggaat
     8041 ttctccaagg cttttactgg accaggccca aatggatgct gggcatcagc cttgctgtca
     8101 tcacgttctg aattgatttc caaaaggcta aacaagtcag ccaagaggtc ttctttgcgt
     8161 ttttctactt ctgctgtaaa atcaattgct gtcatttttt cttctttcta tcttttctcg
     8221 atgatttcat ctactggcaa gcggtagctt ggttccaatt tttcgtctgt ataacccact
     8281 gtgatcaaga gttctgggcg gaaacggtct tcgatttcca aaacttcatt aacttttgat
     8341 ttgtcaaaac caagaataat gttagaacca attccttggt ctgtcaatgc aagaaccaag
     8401 ttcatggcaa ccaaacctgc attgagagct aggtagtcgc tgacttgttg ttcattgtag
     8461 cgtgcaaatt cagctggcaa atttttcatg aagtattgaa gttgttcttc tgagaaattg
     8521 ttagcaccac caactcgggc aatcttacga gcgcgtttgg caaggtctgt atctgtaaat
     8581 agggcaatgg ttacaggtgc tgatgatacc tgctcaaagt ttgaaccata agccaacttt
     8641 gccagttcag catttttctc acgaacaact acaaatttcc aaggctggct gttgtgggcg
     8701 cttggcgcca aggttgcaat ttcgatagcc gtacgcacat cttttggatc aacaggctta
     8761 tcagtgaaat gcttggtcgc atgacgtttt ttatttaatt caagaaattt cataatcgat
     8821 ttccttttct aattctactg cttccattct accataattt gaaagagctt gcagggattt
     8881 ttcatgatta agtttttcta tcacagacat aaaagatata tattggttta tcaggaaagt
     8941 ttctgataaa ctagcaagta ctttacattt gaatggagac agtatgaaaa aatttagcct
     9001 attactagct atcctaccat ttttggttgc ctgtgggaat caagctacac ccaaagagac
     9061 tagcgctcaa aagacaatcg tccttgctac agctggcgac gtgccaccat ttgactacga
     9121 agataaaggc aatctgacag gcttcgatat cgaagttcta aaggcagtag atgaaaaact
     9181 cagcgactac gagattcaat ttcaaagaac cgcctgggaa agtatcttcc caggacttga
     9241 ttctggtcac tatcaggctg cggccaataa tttgagttac acaaaagagc gtgctgaaaa
     9301 atacctttac tcgcttccga tttccaacaa tcccctcgtc cttgtcagca acaagaaaaa
     9361 tcctttgact tctcttgacc agatcgctgg taaaacaaca caagaggata ccggaacttc
     9421 taacgctcaa ttcatcaata actggaatca gaaacacact gataatcccg ctacaattaa
     9481 tttttctggt gaggatattg gtaaacgaat cctagacctt gctaacggag agtttgattt
     9541 cctagttttt gacaaggtat ccgttcaaaa gattatcaag gaccgtggtt tagacctctc
     9601 agtcgttgat ttaccttctg cagatagccc cagcaattat atcattttct caagcgacca
     9661 aaaagagttt aaagagcaat ttgataaagc gctcaaagaa ctctatcaag acggaaccct
     9721 tgaaaaactc agcaatacct atctaggtgg ttcttacctc ccagatcaat ctcagttaca
     9781 ataacactct tcgaaaatct cttcaaacca cgtcagcttt atctgcaacc tcaaagctgt
     9841 gctttgagca acctacggct agcttcctag tttgctcttt gattttcata ttgagtataa
     9901 gatatattaa aagcgatggg atcagccaat cgctttttta gtttgcattg gttattttag
     9961 gaaactctta caaaaaatca aaaaaattct tcacatcctc tacataccta tgcttttcta
    10021 ttaagcttgc taatagttcc agattgtgcc tctgatagtt atcttcacga cgtagttctt
    10081 catacatttc gataaaggga aaacccttgg atttggcaaa ttctaactca gcctcgtaat
    10141 cataagcgac tttacgaaat aggatattta ccaattcccc atcttcaacc tctatcacgg
    10201 catactgggc acggtgattt tttaacgcct cccaattaaa atagggcatg ccaatcgacc
    10261 ctggattgat gatttgttgc ccttgactac cataacgaag caactacttg tgaacatgac
    10321 cataaactgc cacgtccgtt tccgcatcta gcagttggtc aaatttctct gtatcattct
    10381 caactagcaa gtcaccacca tagtttttgt caggtaaatt atgagagata gaaaagcgca
    10441 atccgtcaat ttctttcttt tccagcaaag gcaagcttcg tagccagaca atcgttgcag
    10501 gatccattcg ctccatcaaa tactgtgtca tacgcaagag ctgaacttcc tgtgggtctt
    10561 ctaagccata ttgcccatct aaagcctcaa ggacacaatc atcccaattg cctcgaacac
    10621 ttgccgtgat aggaagttcc tttaacagag cgaccaagtc atttgcacct ggaccaggga
    10681 ggaaaatgtc tcccatgagc caatattcac tggctccttg agttttagca tctgcaatca
    10741 ctgcttctag cgccgtcgca ttgccatgaa catctgataa aattgcgatt ttatggttta
    10801 ttgtggtttc cttccgtttg gtaatctact ctttcttccg ccacttgaag caagatttct
    10861 gtttcctgcg ggttcaactt ggtttgcaca gcctctgcta ctgctcttgg tactccaact
    10921 tcgacaatct catccacact ggcttccttg attttggtca gagacttgaa gtgcctcatg
    10981 agattttgct tgcgtttagg tcccagacca tcaatcccat ccaattgaga tgagaaagaa
    11041 tttttggagc gcagttggcg gtggaaggta atggcaaagc ggtggacctc atcttgaatg
    11101 cgttggagga ggaaaaattc ctgagaattg cgagacaagt ccaccacttc cagcggatct
    11161 ccaaagagca attcatgggt ttggtgctta tcattctttt gcagacctgc aattggaata
    11221 tccaagccca gttcttcttg gatgacttgc ttagcaatat tgacttgacc ttgcccccca
    11281 tcaatcacaa tcaaatctgg aggagtcaaa gcctcacgct gtactcgacc atagcgtctg
    11341 cgaatgacct ctcgcatact ggcatagtcg tctggcccga ctacggtctt gattttatac
    11401 ttacggtaat cttttttact tggcctgccg ttaacaaaga ccaccatggc tgaaacagga
    11461 ctggtcccca tgatgttgga gttatcgaag gactcgatgc gaactggagt cggaatttgg
    11521 agcaaacgtc ctagattttc aatagctcct tgggtctttt cgactgattt ttctagcagg
    11581 ttaaatttct gctccagact aacacgtgca ttttttatgg ctagattgac cagctgtttt
    11641 ttctctccac gttggggttt gagaatcttg gtatccacca aagccttgac agcttcttcg
    11701 tcaatatcct gcggaatcag tacctcattg ggaactaggt gagatttttc ttgatagaat
    11761 tgtcccacat aggtcaagaa atcctcatct ggatcattga agtaggggaa gagattgaca
    11821 tcgcgctcga tgagcttgcc ctgacgaaca aagaaaacct gcacacacat ccagccctta
    11881 tccacatagt agccaaagac atcgcgattt tggagatcct tagccatgac ccgttgcttg
    11941 gttcgaagcg ttccaatagc ctgaatcagg tcacggtatt ccgccgcacg ttcaaactcc
    12001 atagtctggg ctgccgctgc cattttgccc tttagctcat caatgatttt gttatcctgc
    12061 cctttcagaa aatcagaaac ctcctgggcc atagacttga aataagcctc atccttctta
    12121 cagatggtgt gagccataca ctggccgata tggtaataaa aacagacctt agagggcggg
    12181 ttggtacact tacgaaaagg gaatatccga tccagcaacc gcttgatttc attggctgcc
    12241 cccacatcgg gatagggtcc aaaataaaga cctccgtcct ttttgacctg acgagtgata
    12301 atcaagcgtg gatagcgctc attggtgatt ttgatgaaag gataggactt gtcatccttg
    12361 agcatgatat tgtacttggg cttgttttcc ttgatcaggt tgatttctag gagaagtgcc
    12421 tcaatattag actccgtaac aataaattca aaatccacaa tttcagacac cagagcctct
    12481 gtcttggtat catgacttcc acgaaaatag gaccgtactc gattacgcag atttttagcc
    12541 tttcctacat agataatgga gccattttta tccttatgaa tgtagcaacc agggctggtc
    12601 ggcaagagct ctagttttga tttaatcaag ttgttcatag ttccattata gcaaaaaaag
    12661 tagggaaaga tagtacccta ctcactagtc tcatatattt ttcggaggtt tgcaacatcc
    12721 tcttcctgga taccataaaa ggaacctgct ggttgcatga cagacttctc atctgtatgg
    12781 tccaagccaa tcgcatgacc taactcatgt tctgccgtat ggacaaggcg ttcataggag
    12841 tagccatagt gtggattgga caaataataa tgattcaacc gcaccgttac ggacaagaat
    12901 tgccctgcta agagattagt ttgactttcc gcctctcctg ccacaggagt gcctccgtcg
    12961 ttcatctccg tagccgtaat atccgcctta ctagactcag tcacgagttc aaagttaaaa
    13021 gcaccagttt gattccagtt ctggattgct tctaaataag cctcttgaaa gcgtgaatcc
    13081 atctgcggat ccaggtaaat acgagcagag gcctgtgccc accttccttc agaatgctgg
    13141 tgactatctg tctggcttaa cttaggcagt tgccctgttt tctcccattg gtcaatactt
    13201 tgttgaccaa tttttactga ccgttctgct tgctttagcg ctccttgaaa atccccgttc
    13261 agataccaga gaagtccgaa agcaagcaca cataagagca caactatcca aaccagacgc
    13321 caaaacaaac gccacacaaa agaaaagaaa gcccctatca aacgaaaaag ccagcgcatg
    13381 tctctccacc tttctagcaa ataagaatta ttttactaaa agaagctaaa agaaatctaa
    13441 atactgactt tttcatctta acgtcctact atttttttga gtaactggat agctttgtat
    13501 tttaaaggcg atggatggta acggaaagta cccgctttgc gtacaatata gccattaaaa
    13561 ttctgtttaa aacgcaaaac accatcactt ccatcaaaaa tcccttgaat gcctaggaaa
    13621 ttgtatttag gtattccacg ttttatgctt tccaacataa catatttttg aagcagtgca
    13681 ggggcataga acttattaaa ctcagtgtag gaaccactaa agagataagt cgtttcctga
    13741 ggcatataaa caaataaact cccagctaaa acaatatctt cttctccata tttttcaatc
    13801 aagtctcgcg cttctgcttt tcgaacttca aacgtttcaa attgactaga atattctctc
    13861 agttgatttt gttttttctc agaatgagga tttttactca aatcaagtcg caacttgtcc
    13921 aagttttctt ctagtttact ttgttcacct tgcaatttgc tcatatactc cgaaaaattc
    13981 aagcttgcta tgagaaactc cgcttgttct ccaaaagtat cataaaaata ctcataatat
    14041 tctaaacttt tatcactata ttctctacgt tcagaggttt cttttgttat attcttaaaa
    14101 atcgatagtt cttcacgttt taactttttc aaccgaatgc caaaggtttc agcctttttc
    14161 accaagggtt tacccttttt gctaaaactt ttaagcaaac tcttttcagt taattcagtt
    14221 aaatctttat agtataacca atctggttct ccacctgggt aacctgttgt taagccatca
    14281 aattgataac ctaaatcagt caaaccttga ataatacttt ttttctcagc atctattgga
    14341 ttaccttggc tatcaaaagt ttgataagtt tcataaggtt ttacaagcaa ctctaataca
    14401 ccattttgct tggcatattc ttttaactct gcataaaaaa ctggaagagc atcttgttgg
    14461 gtataaatcg gccccgaatt gagttccata tgcagaccac ccagcatggg caggctataa
    14521 accagagctg caacttgaat ttctccttct tgtttcaaag caagataaac aattcgagcc
    14581 cctctttttt ctagcaaatc ccccatctgg acagattgca taaaggaacg agaagaaacc
    14641 tgatcagaat aagtctgaaa ctcttctttc gtgagtgttg ttagtgccat atacttactt
    14701 tctatgtttt tttcttaatg ttttacggaa atcaagagca agtcttaaca gaggatagag
    14761 aggatgagtg ggcattgtaa attcacccaa gtattcttca atcgttggat taaatttttc
    14821 cttaaaatga taaagtccac cattgagaga gttttcaaca ccacctaaat tttgccagac
    14881 catacctcgc tcaaaggcat agcgagccgt ttcataccat gttaaaattg gtgcattgta
    14941 acgtttaaaa tcatcatcca taccagcata tatattgaca gaggtagtac caaattccaa
    15001 actcaaagta gccgctaaag gaactctcgc ttgacctaca tctatatatt cctgcaagaa
    15061 ggtcaattcc tctaacaaac gttctttttc cttcttctgc gcttctactt ttgaagttcg
    15121 agtcgactca gtaaacgtct cttccaaggc tctatttttc gctaactgtt cttctaactc
    15181 ttgcgaacgt ttagaaacat ccaaggtggc caaggtgata taggccttgt ccttaaaatt
    15241 atctaacaat tttttataat aggcttcatt cctcaaatga atctcttttc gcttctcagt
    15301 ttttttcatc aactccgaaa atgaatctaa tagttccagt ccaccatatt gaatctcaag
    15361 ccctttgttt cgtgctgttc gaatagcctg ttttgttgac ttggaaagtt tatcttcttc
    15421 aaaattttcc ttgtatattt tcgcctgaat acgaggttga atggtgtctc ccatttcctc
    15481 cgtttttcct gaccacctta ctcccatttg ttgcaaacta tcaataatag ccagattttc
    15541 aggaaattct gtcttttcct gattgattaa actttgagat aggcaaatac ttgggtcaaa
    15601 agtcacaaaa accgctctct tactgcgagc ataggactta atagactgaa tgacaaaatt
    15661 caagagttct ttatccccat aatccaatat aggtcctctt gggatgtaaa acattttata
    15721 gcctagcgga agagttctaa tcaaaatact agctgtcgcc agtaattttt cttccctgta
    15781 aacaccaaac ttctcatgtt gccaattaga cttaacttcc tcccaagcac tactttgtaa
    15841 tacattggct aattcatgtt ctttgacaaa ctgatcatat tctaatgtgg gaatgccaat
    15901 ttgataacgg tacatttcct actctctttc ctccagtatt ttattactat tctactactt
    15961 tcgtttgaaa agtctagtga aaacatattt tattttagtt ctactccccc tatgctgaag
    16021 ttcaaactaa gtcagtctct cttctaattt gaaatcaatt ggtagggttg ttaaaaaaac
    16081 ttccaattgt ttttcccagt agtaccactc gtgagttcca gcgctatggc tataggtcac
    16141 atcaaaacct agttttttga gatttttcac tgcgagatta ttggcttcgt acaagaaatc
    16201 ctgttcgcca caccacgccc aaagtttggt ctttttatcc gattttttag ccagactttc
    16261 aagagaatag ggactagttg tccagtcttt aatctctcca aaaacacctc tccagtaggc
    16321 tggacttccc agattttgac tttcaggaga aaagttttga aagctgaggg cacctgaaaa
    16381 gctagctgca tgagaaaaac gatttgtcgc aagagccagt ttgaagcagc cgtagcctcc
    16441 catagaaaga ccagcgataa aggtcttttc acgcttgcta gtcatattag ggaagaagcg
    16501 tttcagaacc tgtggcaatt cctctgctag agccgtgtag tagtcaaaac catactgggt
    16561 atcggtgtac caaccattgc tggtattggg cataacaacg atgagattag ttcctcgaag
    16621 caagcgttct acattggtcc gcttaagcca actattatga tttccagaca tcccgtgcaa
    16681 aaggtacaag acgggaatat cttcacactc tggttcttcc actcgattgg catcagggta
    16741 gaggacattc accccccact ccatatccaa tacttgtgag taatactcga ttttcattac
    16801 tgccatgatt ttctccttct atttttctat aagaaaaagc cgaaactcga ctttctctct
    16861 gtttatttca acgattattt tgcttccatg actactggta aaatagctgg acgacgcttg
    16921 gtttggtcaa agagatactt ggtcaaatta tctcgaacct tccctttaag atctgcccag
    16981 tcaaagtcat ctccttgaag atactcttct accgtttggt taatcaattc tgaactttca
    17041 cggagaatat cgcgactctt cttgagataa acaaatccgc gcgtatgaac acgagcccta
    17101 gccacaattt tcttctcacg acggttgact gtaatagcca cgatgaaaat tccatcctct
    17161 gacaagacct tacggtcacg aagaacaaca tttccaacat caccaatggc attcccatca
    17221 atcaagatat ctcctgctga aaccgatcca gctggaacaa agtctccatt ctcgtaagcc
    17281 atggtcgtcc cctttttagg aatgaagatg tgttctggca acatcccaac tgccatggca
    17341 gccttagcgt gagcatccaa ctcacgatac tccccttgga cagggaagag gtacttaggt
    17401 tgcaaaagat tgatcatcag ctgcaaatca cgcacatttc cgtgccctga tacatgtaaa
    17461 ctttgggtaa tcaatttgac aaccccacct gcctgataaa tcatattttc cacacgcgca
    17521 acaaaggctt ctttagcaat agacggagcc gtagcaatat agaccaggtc cccatccttg
    17581 atttctacat aacgatggcg accaatcgac atcttacgaa gtccattgat aggttcaccc
    17641 atacgacctg tctcaagaat aatcaactca tggtcttcaa agcgagacat atctttaggc
    17701 ttaatcaaga gaatttcgtt ggctaaagac aacttcttaa gacgaatcgc tgtgcggacg
    17761 atattttcaa tatcaaatcc tgtcaagacg atacgtcgac ctgttttatc cgcagcgtca
    17821 aaaatctgct gaatacgaga aagattactg gaaacagctg caacgatgat acgaccttcc
    17881 cagtcagcaa tagtttgggt aatttcatcc ctaacttcac tttcactagc cacctgaata
    17941 ttgctgtctg cattggccga atcactgagg agagccagga cgccgtcacg accaatctct
    18001 gccaaacgag caaaatcagt tgcataagat tcactagccg tttggtcaaa tttgaagtca
    18061 cctgtataaa cgatgcttcc ttccgatgtc ttcaagacaa ttcccagact ctctggaacg
    18121 gagtaagtcg tagggaagaa ggaaaccact gtcccaccaa aatcaatctc cgtattctca
    18181 tcaatgacat ggaaatcatt aaatttctta acggcatcat ttcctttgac aaagagcttt
    18241 gccaactcaa tggtcaactc agacccaaat acaggaactt tagcctctgc caagagataa
    18301 ggtagagcac caatggcatc cgcatgcccg tgggtcaaga aaaccccagc aatacggtcg
    18361 ctattttcaa aaaggtaatc catgtttgga atcaccacat cgacccctaa ttgttcattt
    18421 tcaggatatt ttaaccctac attcaaaaca aaaatggact ctccaatttc agcaatgtac
    18481 atattttttc cattctcacg cacaccacca agtgttgtta aactgatatt actcattttt
    18541 cctccaaaat cttagtttat cttgattata gggcaactct accctcttat tctaaaagtt
    18601 ctaatacttt cagccgaatc ttttcctacc attataccat ttttttgatg attttcaaaa
    18661 tgaaggtctg ttttcaaaaa agagttgatt tcccaactct ttcctaactt ctactctttt
    18721 tccatcaaaa aatcataaat ttcttgcacc gtacccagcg ccatcatttc ttggtcttta
    18781 acggtttgtt tgagattttg gtaaatttga ctttctccta tctcccgctt tggcgcctct
    18841 ttattgactg tcatcacacc atcttttatc gtcacaaagc ctagttcttc aaatacttga
    18901 atcatcttga ccagcaagat ttgttgaata ttaagatatg cagccaaatc tttcagcttg
    18961 tagcgaatat caaactctgg gaactggtaa atagtcttgt acaatttggc aaactgatct
    19021 ctagtcccat aacctgtcag ataataggcc ttgtcaatat catttttgaa atagacagca
    19081 gagaaatgct gttcctgaaa aatggtcttc agctgagtaa tatcctctgg aatgtttttt
    19141 acgacaatag cttcactagc cgcaagattt ggcagttctc caggaaaatc caagactgga
    19201 acaccttctg gcaagactgc attttttcca cgaatgttaa aaagttgaac accttccact
    19261 cgcgcatcca ccatcatcaa ctggagggca gtttggccat tccattggtt gacagacaat
    19321 ttaaccgcta actctagatt cttggtttga gaaaactctg tcgcccatct gccttgacca
    19381 aaggctacca cttcaaaact cgccccaccc ttggaaattt tcagctttag atgggcatta
    19441 cctgccccca tagtacgagc actttcgacc tgaaaattct tgatataaaa aataggtttc
    19501 tgattatcca ttccaaaagg agctaaacgt tcaaaacttt tgaccgtttc caagctaagt
    19561 gcctccaaat ccaactcttc atctaggttt aacttattct tgccaccagc atctgcacct
    19621 ttttcacgaa cataatcttc caaaacctga gataaatctg agagttgctc aacttccagc
    19681 gtcatacccg ctgcacctgc atgacctcca aaggcgatga agaggtctcg atggggatcc
    19741 agagcttcaa aaatatcgac cgcttccaca ctacgagcac tgcccttggc acgaccgtct
    19801 tctatattaa gaacaatgac tgtctgtccc aattcttcca ataaacgacc agccacgatt
    19861 cctagaaccc caggattcca gccttccttg gccaagacct gaaccttctt ctcaggatcc
    19921 accatggtct tggcttcttc atagatagac tgaacgattt ccttgcgctc ttcgtttttc
    19981 tggtgaatca taagggcaat ctcatgcgct tcctcatcat caaatccagt caacaaatca
    20041 atggcaggat tgggatcatc caagcgaccc aaggcattca aacgaggagc aatctggaaa
    20101 ccaaccgttt cttctgttac ttcgttggca gcaatcccag ccatgtccag catttcttgc
    20161 agaccaatgc gctgggtatg acccaacatt tccagaccat attgaactaa gatacgattt
    20221 tcatccgtca gactcaccat atctgcaata gttccaatag cgaccaaatc aagcaattcc
    20281 acttgcactt cttctaacag ggcacaagcc aacttgaaag caactccaca accagccaaa
    20341 tatttaaaag gataatccgc atctggatgt tcaggatgga caatagcata agcatctggc
    20401 agggtttcag gcatggaatg atggtctgtc acaatgacat ctactcccat agactgagcc
    20461 aatgcaatag cctcatgacc agcaacccca ttgtccaccg tcacaatcaa ggaaattcct
    20521 tcttgctcga taaagtattt ataaacacta gcattagggc catagccatc ggtaaaacga
    20581 tttggcaggt aaactcggca ctccgcacca agttgttcca aactttcctt cacaatagaa
    20641 gccgaagtca tgccatccgc atcgtagtct ccataaacga gaatattttc cctttcttca
    20701 atagcctgac gaatccgctc cactgccttg tccatatcat ggagcagata agcatcatgt
    20761 aagtcctcca aggaaggttc taaaaacttc ttcagacttt cttggtcctg aatccctctc
    20821 tcaaacaata accgagccac ctcaggaccc agtccagcct tcttggctat ctttgtaaaa
    20881 tccgcatctt ctacctgcag ggcaaactgc cattcataag taggtgttat caaaaagaca
    20941 tccactcctt ctaagaattc tattgcttca ttataacatg atttagacgt tttctctatc
    21001 cagattgctt ttttataaca tttcatagta tattgaaact agaatagtac acctctactt
    21061 ctaaaatatt gttagaaatc gatttgactg tcccgatcga tttttcctgt tcttatttca
    21121 ctatagtgaa ttttttcaga tatggtttga caagaacatt cttttattgc agaattgtgt
    21181 tataaaatct aacataaagg agttctctca tggctttagt agaatttaaa aacgtcgaaa
    21241 aatattacgg agactaccac gcactccgca acatcaatct ccgttttgaa aaaggacaag
    21301 ttgttgtcct gcttggacct tctggctctg ggaagtccac tcttatccgt acgatcaatg
    21361 gtttagaggc tgttgacaaa ggaagtctcc tagtcaatgg gcaccaagtt gctggtgcca
    21421 gccagaaaga tttggtacct cttcgcaagg aagtcggcat ggtttttcaa cattttaacc
    21481 tttatccaca caaaacagtg ttagaaaacg tgacacttgc acccattaaa gttctaggaa
    21541 ttgataaaaa agaagctgaa aaaaccgcca aaaaatatct ggaatttgta aatatgtggg
    21601 acaagaaaga ttcctatccc gccatgctat ctggtggaca aaaacagcgg atcgccatcg
    21661 ctcgtggtct tgctatgcat ccggaactcc tcctctttga tgaaccaaca tctgctcttg
    21721 atcctgagac tatcggagat gttctagcag ttatgcagaa actggcgcgt gatgggatga
    21781 acatgatcat cgttacccac gaaatgggct ttgctcgaga ggttgcggac cgcattatct
    21841 ttatggccga cggagaagtt ttagtagata cgacagatgt cgataacttt tttgacaatc
    21901 caagcgaacc tcgtgcccaa caattcctca gcaaaattat caaccacgaa agtgacaaag
    21961 tcaaataagg aggcgcctat gaaaaaaaat tctttttatc agcattattg attagccttt
    22021 tcggccttgc tgctgccaaa ccagtccagg ctgatacaag tatcgcagac attcaaaaaa
    22081 gaggcgaact ggttgtcggt gtcaaacaag acgttcccaa ttttggttac aaagatccca
    22141 agaccggtac ttattctggt atcgaaaccg acttggccaa gatggtagct gatgaactca
    22201 aggtcaagat tcgctatgtg ccggttacag cacaaacccg cggccccctc ctagacaatg
    22261 aacagggcga tatggatatc gcgaccttta ccatcacgga cgaacgcaaa aaactctaca
    22321 actttaccag tccctactac acagacgctt ctggattttt ggtcaataaa tctgccaaaa
    22381 tcaaaaagat tgaggaccta aacggcaaaa ccatcggagt cgcccaaggt tctatcaccc
    22441 aacgcctgat tactgaactg ggtaaaaaga aaggtctgaa gtttaaattc gtcgaacttg
    22501 gttcctaccc agaattgatt acttcccttc acgctcatcg tatcgatgcc ttttccgttg
    22561 accgctctat tctatctggc tacactagta aacggacagc actactagat gatagtttca
    22621 agccatctga ctacggtatt gttaccaaga aatcaaatac agagttcaac gactatcttg
    22681 ataacttggt tactaaatgg agcaaggatg gtagtttgca gaaactttat gatcgttaca
    22741 agctcaaacc atctagccat actgcagatt aaggaggaca ccccatgaca gatttatcat
    22801 cttggacagc ctattttcag gattttggac aatttttcaa tggtttcctc ttcacccttg
    22861 ccctagcgat tggatccttt atcctcgcca tggtcttagg aatcttcttt ggtgccatgt
    22921 caaccagtaa acgtccaatt ttacgcattt tagctcgcat ctttgtggaa ttttaccaaa
    22981 acactcccct cttggtgcag tttgttattg ttttttatgg cctacctctt atcagtgacc
    23041 atactatcat gattccgatt tattggacag ctgttctctg cgtgggactc tatcacggcg
    23101 cttatatcgc tgaggttatt cgttcaggga ttcagtctat tcctagtggt cagatggagg
    23161 ccgccttatc gcaaggtttt acctatatca gtgccatgcg cttgattatc ttgcctcaga
    23221 ccttccgcat cattctccct ccattgacca accaaattgt taacctcatc aagaacacct
    23281 ctactgtagc tatcatctct ggagtagact tgatgtttgt gactaagtct tggtcggctc
    23341 tcaacggaaa ctatatccca gcctttttag gtgctgctct tctctacttt gccctatgct
    23401 tccctgttgc ccagtttggt cgcaagatgg agcaagccaa caaaaaagcc tattcacttt
    23461 aggaggttac tatggaatct attttagaag ttttaacccc agataaccta gtctttatct
    23521 ttaaaggatt tggcttgacc ctctatattt ctctgattgc catcatcctc tctactatca
    23581 tcggtacggt gctagctgtc atgagaaatg gcaaaaattc tgtcttgcga atcatctcca
    23641 gtatttatat cgagtttgtg cgcaacgttc ccaaccttct ctggattttt actatctttt
    23701 tggtgttcaa aatgaaatcc acaccagcag gtattacagc ctttactctc tttacatcag
    23761 cagccttggc tgagattatt cgaggcggtc tcaatgccgt agacaaggga caatacgaag
    23821 caggaatgtc acaaggcttc acctcagccc aaatcctcta ctacatcatt ctcccacaag
    23881 ccatccgcaa aatgctacca gccatcattt ctcagtttgt taccgtgatt aaggatacca
    23941 gtctcctcta ctctgttatc gccctacaag aactctttgg agccagccaa attctcatgg
    24001 gccgttattt cgaaccagag caggtcttca gtctttacat cctgattgcc ctcatctact
    24061 tcagctttaa cctagcaatt tctagcctgt ctcatatgct agccaaacgt tggcaacaag
    24121 ccgcagaata atactcttcg aaaatctctt caaaccacgt cagcttcacc ttgccgtata
    24181 tatgttactg acttcgtcag ttctatccac aacctcaaaa cggtgttttg agcagcctgc
    24241 gggctagctt cctagtttgc tctttgattt tcattgagta taaacagcca ctctccaatt
    24301 caattggaga gttttttgat gagagtagat atttatagac ctcaatttga caccatacat
    24361 tttctatgat aaaatgtaac aaattcattt acaacaaatc gaaatctaaa tgaaatggaa
    24421 ttttctatga aatcagtcaa aggactactc tttatcatag ctagttttat cttgactctt
    24481 ttgacttgga tgaacacttc tccccaattc atgattccag gactagcttt aacaagccta
    24541 tctctgactt ttatcctagc cactcgtctc ccactactag aaagctggtt tcacggtttg
    24601 gagaaggtct acaccgtcca caaattcaca gcctttctct ccatcatcct actaatcttt
    24661 cataacttta gtatgggcgg tttgtggggc tctcgcttag ctgctcagtt tggcaatctt
    24721 gccatctata tctttgccag catcatcctt gtcgcctatt taggcaaata catccaatac
    24781 gaagcttggc gatggattca ccgcttggtt tacctagcct atattttagg actctttcac
    24841 atctacatga taatgggcaa tcgtctcctt acatttaatc ttctaagttt tcttgttggt
    24901 agctatgccc ttttaggctt actagctggt ttttatatca tttttctata tcaaaagatt
    24961 tccttcccct atctagggaa aattacccat ctcaaacgct taaatcacga tactagagaa
    25021 attcaaatcc atcttagcag acctttcaac tatcaatcag gacaatttgc ctttctaaag
    25081 attttccaag aaggctttga aagtgctccg catccctttt ctatctcagg aggtcatggt
    25141 caaactcttt actttactgt taaaaattca ggcgaccata ccaataatat ctatgataat
    25201 cttcaagccg gcagcaaagt aaccctagac agagcttacg gacacatgat catagaagaa
    25261 ggacgagaaa atcaggtttg gattgctgga ggtattggga tcaccccctt catctcttac
    25321 atccgtgaac atcctatttt agataaacag gttcacttct actatagctt ccgtggagat
    25381 gaaaatgcag tctacctaga tttactccgt aactatgctc agaaaaatcc taattttgaa
    25441 ctccatctaa tcgacagtac gaaagacggc tatcttaatt ttgaacaaaa agaagtgccc
    25501 gaacatgcaa ccgtctatat gtgtggtcct atttctatga tgaaggcact tgccaaacag
    25561 attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt caaataagta
    25621 atcatactct cctgggattt cccaggagag ttttttatag ccaaatgaaa taagaccgga
    25681 acgatggaat tttggcattc aaatcaattg ataagaatat tttatagtgg agtgaaatag
    25741 gatacgaaca aattgattag gaaaatcaaa tgaatttata gaaatctttt agcagttatg
    25801 ttgtcctatt ctagtttcaa aacgctatag aagcagcatt gtactagtct agattcagtt
    25861 tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc aggttttatc
    25921 tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat acgttcttca
    25981 accgatgctt ggatagcttt tacaccgtca gccaagaatt tacgtgggtc gaagagtttt
    26041 ttcttgtcgt attctgcttc gtttgcttcg tagtcacgag caaatttacg agttgcgtta
    26101 gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag tttgatagct
    26161 gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg gaatcctgga
    26221 agagcttctg tcaatttttg caagtggtca aggtcaagac cttcccagtt tactgggtaa
    26281 gggccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc aaccattgct
    26341 ttagcgtctt cgattggagc caattcacct ttaccgatga ttccgtcttc ttcaccaccg
    26401 atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc aacaacttct
    26461 ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat gattgaagta
    26521 taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag gtggatagct
    26581 actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg agcaactttg
    26641 taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg agctttttta
    26701 gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt aaatccacca
    26761 actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac gattgccatt
    26821 ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca ttttatcact
    26881 ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct aactccatct
    26941 atgtaaaccc tttctctccc tagtcttggg cgacttttgg aaaagctata aagaaggtta
    27001 aacgattctc ctccatctcg aaacgataag ctaatttttc atgttctaat agactcttaa
    27061 ccacaaagag ccccatacca gaccccttga ccttgcgact ggcattgtca gaaaaagact
    27121 gggctagttt ttcttgttcc tctgagctac agctattttc gataaaaagt tctccttctc
    27181 tttctccaat tcgaactaag ccacctggaa cagagtgctt aatggcattg ctgatgagat
    27241 tagaaagaat caacttcata actgatgggt ttagataagc ctgctgatgg gtcaaactat
    27301 tgtctatctg gagctctctt tccttggcta gcaaggcata atctttgacc agattttgcg
    27361 tcatctggag gaggtcaatt gtttccctat catctcgcaa ttcctgcaca gaagagaggg
    27421 aaagtatctg cagaacatgg tgattgagtt catccacaat ccccaaggca actcccagat
    27481 actggtctct atccttataa cgaccgatat tctctctcat attttcgatt aggattttca
    27541 aactagccag cggtgttttc aattcatgag aagctcctcg taggaattcg accttcatct
    27601 tctccagctg gagaatggct tcattctttt catgcaagtc cgcaataaca gtcaagagat
    27661 gctggtagag gctattgatt tgttccttga gattacctat ctcatcctta gaatccacgc
    27721 gcaatcgcac ttgggaatcc aggtccatca tccgacgggt cacccgcttg atttccaaaa
    27781 tcggtgcaac aatagtccgg gcgtagatgt aggccaccaa gagggaaatc agaaaggagg
    27841 ccagcaaggt atagggaaga aactggagac tgatttgctc cgcttccttt tgtaaatcca
    27901 tggaagctag aaactggaga atcatagtac caccgtcttg cgttttcacc tcgcgctcct
    27961 caataaagag agaggttgtc tggcggtctg tgtccagagg aagactgtcc ttgacttcta
    28021 acttgtcctc ggtcatctca cctttgacgg tccccttgat atcactagtc tgggaataca
    28081 agtctaacac ttgctcgata ctctgcctat ctttcccttc tagggactgg gcaatggctg
    28141 ttgccttttg accaatggtt tcctgacgat gactcagata agtcgaagga aaaagaaaat
    28201 aaatagctaa atgaaggcag ataaccagaa cactaaatat cgagaaggta tagataaata
    28261 tctttgcaaa taaacctgtt cgtttcattt tcgctccaat ttataaccaa cattgcgcac
    28321 agtgaggata caatccaagt ctagcttttt ccgcaattcc ttgatataaa catcaataac
    28381 acggtcaaag ggaacctcat ctgtcgcttt ccagacggca tcgataatct gagaccgagt
    28441 caaggcccgg ccttcatttt tcactagata gtccagaatt tccaactctt tggcattgat
    28501 aggcacttct tgacctgcga ggcttgcact gtagctttca aagtccacct tggtatcctt
    28561 gtaagaaaag attcgtcctg tatcgtagta gcgcttgaaa atcgcgtcca ccctcacttt
    28621 taagagggag agagagaaag gtttttccag atagccatct gccaaagagg caaaggcact
    28681 catcttgtat tcctcatcct gaaaagctgt caacatcaag acaggaacct gactggtttt
    28741 acgaatctca gctaggactt ctaagccgtt aagcttgggc atctggatat ccagtaaaac
    28801 cagggccacc tcatagctag aaaattgctc cagagcttcc tgaccgtccg ctgcctcaat
    28861 agtttcatag ccacaatccg tcaaataatc actgaccccc tcacggatca tctcttcatc
    28921 ttctacaatt aaaattttca tactttaact gctctctatt ttttattttt cttagaataa
    28981 atacctactc tattttctat tatagtctct tgctggcctt ttgtctgtaa gcaactgatc
    29041 actagataaa acgttgtgaa attcctttct cataaattcc ataactttag tatattatat
    29101 ttaagcacta aagtacaaag aaagcaactg aaagcaatga ttttcaccac tgctttcaga
    29161 tttattttga attgttaaat agctattcct atccactatt cttgaataga aacacaagat
    29221 gcaatcttta ttccagactc attttttcaa atttattcac catccagcaa gagctctttt
    29281 ggttgttttc taaggagatt gcttgaagca agcgccataa cgagaaccac tagaaccaag
    29341 gcaaggacaa aaatgatgat aaagtctgat gtctgaatgg aaatgtctag gctcgacaag
    29401 gtcttgctaa agccatctac ttctgcaccg ccaccaaggt tagaggcttg agccgcctta
    29461 ctagcctgtt tggcaacacc tgaagtcaca ttggcaagga cagtgtttcc aattgcacgg
    29521 gcagtgtaat tagctaggaa gtaagcagaa actagagcag ggatagcaat caagatagat
    29581 tcggtgatga attgacccaa gatacttgcc tgcttgaggc cgatagagag gagaattccc
    29641 acttccttgc gacgggcgtt gatccaaagg ctgagcaaga gggcaaggag gagaactgag
    29701 aagctcaagc taccccagaa gaggaggttg gccatcttgt acataccaga gatagattgc
    29761 tcaagagctg ggtagttaga ggagctcttg acgagtgtgt agctcttcca gttgatacca
    29821 ctgatgccat tcaactcttt cataacatca tccaagttct tgtctgctgt tacaaagaag
    29881 gttgcgtccc cataaatggc tgtgtcttct gtgtatccat aaagttttgc agcagtgtga
    29941 atgtctgtaa tagctgtgtt ttcgtaaagt tcttgtgagt aggttactgc tgacttatta
    30001 tgaccatcaa agagtccctt gattgtcact tcaactgttt ccttggctcc tttttcatta
    30061 tctgcatcgt agatattaga gtccagttta accttgtccc ctactttcca gccgtgtttg
    30121 gctgccaagt ccttgtgcaa gaggatttta tccttgtcgt cgttggttaa gtgctctcct
    30181 tcgactagtt tataagaacc agagacaaac ttgtcttctt tagaggagtc attgacacct
    30241 gtaatcatca agctacttcc aaaacgtttg gcacgatcag cagtgagatt cttcttggtt
    30301 tctggcgttt caatcaggtc atatccagtc aaatctccga tagcgttgat acgtttgaca
    30361 taagactcaa tggccttgtt ttcggtgatt tttttgatgt cttcaccctt gatattccca
    30421 gcaccgcgag gcgttccttg gttgacgcga cgattgattt gcatggagaa gctattggtg
    30481 atatttttaa aggtctcctg agaagccttg gcagtagctc ccttgattga caagccgacc
    30541 aaactcaagc tcgccatgag gagaataatc aggaagatga caatcgattt gaaaaacttc
    30601 cttgtaacat aggcaaatgc gttgtgtaac atagattccc tttctagatt ttgttttaat
    30661 cattctatta aagtaagctc aaattattta ctagtattgc gcgtttcagt cagtttctta
    30721 tcctttaatt caagtgtaat atctgacgct tgtgccactt ctttactgtg agttacgaca
    30781 atcacacatt tacctgtttt ctgggcaagt gatttgagta gttcgacaat atctccagca
    30841 gttttaggat ccagatttcc tgttggctca tcagctagaa taactggagc ttctgagacc
    30901 aaactgcgag caatggcaac acgttgctgt tgaccacctg ataactggag aacattccgc
    30961 ttgatctggc tttcatccaa accaagctca agaagtgtat tcttgcttgc ctttttgttg
    31021 accaatcgga tattttccag cggagaaaga taatctatca agttataatt ttgaaagacc
    31081 agggaaatat ggtgcatgcg atggtaagaa tagcccttct tacgaatatc ctctccttga
    31141 aaaaggatag aaccttcaac aggactatct agaccagcaa gtagggacaa gagtgtggat
    31201 tttcctgctc ctgactcccc aataatactg taaaattttc cgggttcaaa attataattg
    31261 atctgatata ggactgcttc agcagtattc ttataacggt aggtaacatc ttgtaattgt
    31321 aataaagtca tgatttctcc ttcttaacta atagatgata aaatttcttt cggtgatttt
    31381 ctaaataaga ataggaaaca aagggctaca gataagcaac taagcagaac tagaaaaaca
    31441 taggattctg caaaagataa gatgctagtt gataaactgc ttgctttggc tagtgtatct
    31501 tgtaagcttg cctgatctcc acttgctagt agagtttgga gtaggtaagt tgtgattccg
    31561 tttcctgcaa caaatgctgg aagcaaagct ccaagagata ccaaaactac ctctaaacag
    31621 aattgtagga aaatcgagct cttgcctttt ccaagtgcaa gtaaaatccc cacttcatag
    31681 acccgttctc tcaaccagag agacaaaacc agaattaagg ctccagctcc tgctatcaac
    31741 atcccataaa ggaagatggt caggaaggtt tggaaagttg caactgagtc tttgatttgt
    31801 tcaaaagcct tgttttcctt ttcgacttgg tagccttgat tttccaaggc caagttttct
    31861 acctgcttca tgagtccgtc catttcctta ggattttcta catagaagcg tgctgcactg
    31921 acttgagctt cactattgcc caaaagggtt tggctacttt catagtctgt aaagacttga
    31981 ttttcactga agtcagaaga caagcctgtg aatttctctt gttttttacc agaaaagatg
    32041 ccgataatct caaactctac tgtttgtcct tttccagatt cagactgacc agcatccaag
    32101 ccaatcttgt catgaagtga aagaccgttc ttcttagcca attcttcgtg gataaggatt
    32161 ttcttggaat tccctttttg aaggtgtcgc ccttctttta gattgaaagc cgaactggta
    32221 aaggttacat ccttggatga atcctcaaga gccgttaagc taaccaagtt attgtctgca
    32281 gctgataaat catcacgctc cacgctctgc tcgccagtca ctgcttcctt gtcttttagt
    32341 tttgcgaccg tctcgagttc aggagagaca ttttccagcc ccttaatctt gcttacagat
    32401 gctaggtctg acaacttgaa tgtctgacca ttctctatct tcttaataga aaaagatgta
    32461 ttgagtgatt tataaagatt gctttctact gttttgttgg acttcatcag agtcaaacag
    32521 gctgaaattc cggccaataa gaccaataaa atcagaaata aaataaaact tctcagtcgc
    32581 tttctgctga cataagccca agatctttgg attggattca tttgtcacct ccatatttgt
    32641 aagactatta taaaacccaa atatgaaatg tttatgaaat acgaaaaaaa aatatcgagt
    32701 aggggataat ctctagcccc tctcatacca ccgtacgtgc cgttcggcat acggcggttc
    32761 aactaacttt taacgcatgt cgttcaaggt aataatccaa acacgaaacc agtccacgtt
    32821 tttcaaggac tggttttgat atagcacgtt taagtaccga cttctgagct actatagtag
    32881 attgaaacta gaatagtaca cctctacttc taaaatattg ttagaaatcg atttgactgt
    32941 cctgattgat tcgtcctact cttatttcat tttactataa ttgatagtgg tcgccccagc
    33001 cagatacctt atctgctatc catttaggaa cccctaactt aagcaatccc cataatcgtc
    33061 tcgatttctt cttccattgc ttccagataa tcactcgtag gcgagtacgc aagcgctcat
    33121 ctatgctagt gactatactt ttcatattta taattcattc ctttcgtttc actcaaggca
    33181 caacacagaa tgaaaaagtg ttgtgctctt tattttgttt tataataata gttagaaagc
    33241 ctatcactac tacaaatcac ggggaggtga ataagtgagt ggtacagcca ctacctcgca
    33301 tattttgtca catcatttaa cggtacataa taagttgtac catctgaata agttgctaca
    33361 atatcatttg catgctctcc ttcaccttta gcaaaggttg gagctcctac tggataattt
    33421 ttatttgcct ctttcaattt ttcaataatg gcattttttt ctgtatcttt tatattatca
    33481 ggatttttca ctaagatttt gtctggatat gtcggtttag cagaaacaat ttttactgtt
    33541 atttcttttt tattcgaagc acttgtccag tttccagcat tatctttagc atttaatttt
    33601 acagtaattc ctgaactaag aacttcagta gcaggttgat tatcaacatt attcaacttt
    33661 aatttcaaaa gagctgttgc atcagacgtt ttatcaatcg ttgtatataa tgataaattg
    33721 ttattataac cagtttcttc atatttagct gtttgtgagc tacttgaaac agaactgaaa
    33781 ttatgcccac tacctccctg attatcttca atgcttacgt ctaaatgaac ttccccacta
    33841 ttatttggct tagcaacaac tgttatagta aaataacata aaatttgcat aaatagatta
    33901 gggaaatcaa agcaacttct aggaatgttt tatactcttc gaaaatcaaa ttcaaaccac
    33961 gtcagcttca ccttgacgta ctcaagtaca gcctgcggct agcttcctag tttgcacttt
    34021 gattttcatt gagtattagc agtcacagtg tactttccca gcatcaagtc actataactc
    34081 tgcacataaa aatggagaag atggccatcc tcttctccaa atattaa
//
LOCUS       DBEJAQ010000023        33113 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.023, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000023 DBEJAQ010000000
VERSION     DBEJAQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.023"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(382..1944)
                     /gene="guaA"
                     /locus_tag="ACL297_001121"
     CDS             complement(382..1944)
                     /gene="guaA"
                     /locus_tag="ACL297_001121"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGR0071393.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CLYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2086..2784
                     /locus_tag="ACL297_001122"
     CDS             2086..2784
                     /locus_tag="ACL297_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGR0071394.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2827..3723)
                     /locus_tag="ACL297_001123"
     CDS             complement(2827..3723)
                     /locus_tag="ACL297_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGR0071395.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(3732..4667)
                     /locus_tag="ACL297_001124"
     CDS             complement(3732..4667)
                     /locus_tag="ACL297_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGR0071396.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4664..5389)
                     /locus_tag="ACL297_001125"
     CDS             complement(4664..5389)
                     /locus_tag="ACL297_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HGR0071397.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(5473..6108)
                     /locus_tag="ACL297_001126"
     CDS             complement(5473..6108)
                     /locus_tag="ACL297_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGR0071398.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6110..6937)
                     /locus_tag="ACL297_001127"
     CDS             complement(6110..6937)
                     /locus_tag="ACL297_001127"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGR0071399.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6959..7294)
                     /locus_tag="ACL297_001128"
     CDS             complement(6959..7294)
                     /locus_tag="ACL297_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HGR0071400.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7295..8011)
                     /locus_tag="ACL297_001129"
     CDS             complement(7295..8011)
                     /locus_tag="ACL297_001129"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGR0071401.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRAQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
     gene            8524..9135
                     /gene="def"
                     /locus_tag="ACL297_001130"
     CDS             8524..9135
                     /gene="def"
                     /locus_tag="ACL297_001130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGR0071402.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9180..9908
                     /gene="rlmB"
                     /locus_tag="ACL297_001131"
     CDS             9180..9908
                     /gene="rlmB"
                     /locus_tag="ACL297_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGR0071403.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9947..10858)
                     /gene="trxB"
                     /locus_tag="ACL297_001132"
     CDS             complement(9947..10858)
                     /gene="trxB"
                     /locus_tag="ACL297_001132"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGR0071404.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10924..11148)
                     /locus_tag="ACL297_001133"
     CDS             complement(10924..11148)
                     /locus_tag="ACL297_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HGR0071405.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11226..11969)
                     /locus_tag="ACL297_001134"
     CDS             complement(11226..11969)
                     /locus_tag="ACL297_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGR0071406.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11969..12769)
                     /locus_tag="ACL297_001135"
     CDS             complement(11969..12769)
                     /locus_tag="ACL297_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGR0071407.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12927..13283)
                     /locus_tag="ACL297_001136"
     CDS             complement(12927..13283)
                     /locus_tag="ACL297_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGR0071408.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13296..13808)
                     /locus_tag="ACL297_001137"
     CDS             complement(13296..13808)
                     /locus_tag="ACL297_001137"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGR0071409.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(13808..14302)
                     /locus_tag="ACL297_001138"
     CDS             complement(13808..14302)
                     /locus_tag="ACL297_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGR0071410.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14437..14883
                     /locus_tag="ACL297_001139"
     CDS             14437..14883
                     /locus_tag="ACL297_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGR0071411.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14880..15527
                     /locus_tag="ACL297_001140"
     CDS             14880..15527
                     /locus_tag="ACL297_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGR0071412.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15548..16129)
                     /gene="pdxT"
                     /locus_tag="ACL297_001141"
     CDS             complement(15548..16129)
                     /gene="pdxT"
                     /locus_tag="ACL297_001141"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HGR0071413.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16130..17005)
                     /gene="pdxS"
                     /locus_tag="ACL297_001142"
     CDS             complement(16130..17005)
                     /gene="pdxS"
                     /locus_tag="ACL297_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042819 - vitamin B6 biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HGR0071414.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17157..18536)
                     /gene="nox"
                     /locus_tag="ACL297_001143"
     CDS             complement(17157..18536)
                     /gene="nox"
                     /locus_tag="ACL297_001143"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HGR0071415.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18830..19753
                     /locus_tag="ACL297_001144"
     CDS             18830..19753
                     /locus_tag="ACL297_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGR0071416.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19813..20418
                     /locus_tag="ACL297_001145"
     CDS             19813..20418
                     /locus_tag="ACL297_001145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGR0071417.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20437..21681
                     /locus_tag="ACL297_001146"
     CDS             20437..21681
                     /locus_tag="ACL297_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGR0071418.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSS
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21904..22161)
                     /locus_tag="ACL297_001147"
     CDS             complement(21904..22161)
                     /locus_tag="ACL297_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HGR0071419.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22203..24239)
                     /gene="glyS"
                     /locus_tag="ACL297_001148"
     CDS             complement(22203..24239)
                     /gene="glyS"
                     /locus_tag="ACL297_001148"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGR0071420.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24499..25416)
                     /gene="glyQ"
                     /locus_tag="ACL297_001149"
     CDS             complement(24499..25416)
                     /gene="glyQ"
                     /locus_tag="ACL297_001149"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGR0071421.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(25435..26196)
                     /locus_tag="ACL297_001150"
     CDS             complement(25435..26196)
                     /locus_tag="ACL297_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGR0071422.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26469..27311)
                     /locus_tag="ACL297_001151"
     CDS             complement(26469..27311)
                     /locus_tag="ACL297_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGR0071423.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLESLKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTTAVQSGAPRVD
                     DVDF"
     gene            complement(27426..28817)
                     /gene="pgdA"
                     /locus_tag="ACL297_001152"
     CDS             complement(27426..28817)
                     /gene="pgdA"
                     /locus_tag="ACL297_001152"
                     /EC_number="3.5.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050119 - N-acetylglucosamine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase
                     PgdA"
                     /protein_id="HGR0071424.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29055..29423)
                     /locus_tag="ACL297_001153"
                     /pseudo
     CDS             complement(29055..29423)
                     /locus_tag="ACL297_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29644..30621
                     /locus_tag="ACL297_001154"
     CDS             29644..30621
                     /locus_tag="ACL297_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGR0071425.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30618..31700
                     /locus_tag="ACL297_001155"
     CDS             30618..31700
                     /locus_tag="ACL297_001155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGR0071426.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTINLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31764..32794)
                     /locus_tag="ACL297_001156"
                     /pseudo
     CDS             complement(31764..32794)
                     /locus_tag="ACL297_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         9840 a         7376 c         5932 g         9965 t
ORIGIN      
        1 aagcagaata tcatttctct ttttagaatc aaaaggacga gattgtggaa tctctacagg
       61 aacaataggt tgtggcatta aaatatcctc caatagtttt actttactaa tagtatacag
      121 gaggaactcc atgaatgata gataccttgt tatgacgcgc ttactatatt agaaagttgg
      181 ctctctaatt aaattaattc tcattatata tatcaaaaga ccgcagtttg aaaactacgg
      241 tctaattttt tgtccaaatg taaatcactt ggaactatta ttcaatttat tctttttagt
      301 aaatgttccc gttttaatcc cagttacagt ttttaatcct ctgtaaccgt tgattttaca
      361 aagcttttaa tttttttgcg attactccca ctcaactgtt gcaggtggtt tacttgtaat
      421 atcgtagacg atacggttaa catgatccac ttcatttacg atacgtactg agattttttg
      481 aagtacttcc catggaattt tggcaaaatc agcagtcata ccatcgatag aagtgatagc
      541 acggattgca atcgtgtagt catacgtacg accgtcaccc ataacaccga ctgaacgaac
      601 gcctgtgtta acagtgaagt attgccaaat atcgcggtca agtccagctt tagcgatttc
      661 ttcacgaaga atagcgtctg attcacgaac ggtttcaagt ttctcttcag tgatttcacc
      721 catgacacgg atagcaagtc ctggtcctgg gaatggttgg cgccatacga tatggtctgg
      781 cataccaagc tctgtaccaa gagcacgaac ttcatccttg taaagagtat tgagtggttc
      841 aatcaattca aactgcatat cttctggaag accacctacg ttgtggtgtg acttgatagt
      901 ttgagctgta tccgtaccag actcgataac atctgtatat aaagtacctt gagcaaggaa
      961 tttcacatct ttgagcttgc ttgcttcgtc atcgaataca tagacaaact cgttaccgat
     1021 gattttacgt ttttgttcag gatcagaaac gccagcaagt ttgtcaagga aacgtttagc
     1081 agcgtctgct ttgacgatat tcaaaccaaa cttaccaccg agcatgtcca taacttgatc
     1141 agcttcgcct ttacgaagaa gaccgtggtc tacgaagata cagatcaatt gatcgccaat
     1201 cgctttttgg agaagaaccc caacgacaga tgagtcaaca ccacctgata gaccaagaag
     1261 gacacgttta tcaccgacgg tttcacgaat ttttttgatc tgcatgtcaa tgaaattatc
     1321 cattgaccag tcacctttag ccttacaaat gttaagggca aagttacgaa ggatatcatt
     1381 tccgtataca gaatgacgaa cttctgggtg gaattggata ccgtaaatgt gtttatctgg
     1441 gttttcgatg gctgcgtata ggcagtcagc tgatgtacct gtacgaacaa agtcagcagg
     1501 aatctcagta accgcatcac catggctcat caaaacagtc tgttcatcag gtgttgattc
     1561 aaaaagcgct gatggtgtgt gagttagggt tgattgaccg tattcacgat ttccagcatc
     1621 acctgcagga acaacttttc ctccaagttt atgggtcaat aactgcatac cataacagat
     1681 tcccaaaatt ggaattccga gttcgaagat ttctgggtca atatcaaatg aaccatcttc
     1741 atatacagaa tttggaccac ctgatagaat aattcctaca ggattgactt cacgaacttc
     1801 agcagctgaa attttatggc tttttagttc tgaaaaaaca ccaatctcac ggatacggcg
     1861 tgaaatcagc tggttgtact ggctaccata gtccaatacg atgatttttt ctacatcttg
     1921 caaatcagtt gaaatgttgc tcatcttttt cctttctcaa aagaaatatc ttttctcaat
     1981 attctatcac aaataagggc gaattaccaa ctaaacaagg cgaagtttga ctttttctag
     2041 tttattgggg tacaatagag aaaagataga aatgaagtga aaaatatgct acctgcttat
     2101 atgaaaatcc atgatcagat aaaaaaggat attgacgagc accgttgggc tattggtgag
     2161 aggcttccca gtgaaagaga tttagctgag cagtttgcgg tcagtcgcat gaccctccgc
     2221 caagccgtat ctctattagt cgaagaaggc gtcttagagc gccgtgtagg aagcggcacc
     2281 tttgtttcca gtactcgagt acaagaaaag atgcgaggga caaccagttt tactgaaatt
     2341 gtcaaatccc aaggtaaagt tccctctagc cagctcattt cctacaaaaa aaccattccc
     2401 aatgagcaag aagttgccaa gctaggaatt tttccaacgg acaatattat ccgaatggag
     2461 cgggtccgct atgccgacca agttccccta gtttatgaag ttgcttctat tcctgaaaaa
     2521 ttcattaagg actttaaaaa agaagaaatc accagtcatt tcttccaaac cttgcaaaaa
     2581 catggctatc gtatcggcaa atctcaacag accatctatg ctcgccttgc taaagaaaag
     2641 attgcccact atttggaagt tgaaaaagga catgctattc ttggattgac ccaggtttcc
     2701 tacctagaag atggtactgc ttttgaatac gtaaaaagtc aatatgtagg cgaacgcttt
     2761 gaattttatc ttgaaaataa ttagattacc acgcaaaaac tctctgtcac ggacaaagag
     2821 tcttttttat ttttctaaga ataaatcttt caaagaaatc aaagttacag caaacaatat
     2881 cattgccata ccaataaaat caatgggata aaattgttca ttcattatta agaaggcaaa
     2941 gaaaaccgcc gatattggct caattgaagc caacaagctt gacttgaccg gtcctatcag
     3001 actggctcct ttaaggaaag ctgtataggc aaagacagtc ccgataagga taatgcctgc
     3061 aaacgcaagg agaaaatcaa gactagtcgg gatatcggcc tgtagaaccc ctgtaaaagg
     3121 aagggcgacc aaacctgcta tgaccatccc cacaccaatg accaagctgc tcccccactt
     3181 tttaatcaag gctatgggta aaatgatata caaagcataa gtcaaggcag aaaagagacc
     3241 ccagaacagg ccagcaggtg tcatggataa ctggtccaac tgcccatgtg ttgcgatcag
     3301 gaaggttcct ccgatggcga atatgatgga aactatctct cccagtgtcg gtgccaccct
     3361 atccttgata cagctataaa ttaaaattcc gacaggacaa acatactgaa gcaccgtcgc
     3421 tgttcccgca ttggtctcct gaatagcaga cagataggcg aattggttga gaaaaagacc
     3481 aatcagagca aaaataagaa gagacagcaa actctttcta tcctttaaaa agaccagtat
     3541 tttatccttt gcagtagcat aagccaagag catgagaatt ccaccagcga ttaaaagacg
     3601 caagttggtc aagaccagag ccgaaattcc gtgtgccatt aggtattggc cactcgttcc
     3661 tgacaacccc caagcaatac cagccacaac tgttagtaaa gtccctttta aactatttga
     3721 catgtctctc cttactcttc tttgcgaatc aagttcatca cttgattgcg gtctaatatc
     3781 cattgagctc tctcgtcctt ttcataggta cgattcgata ggaaaatagt ggcttcttgc
     3841 ttctgacgat tccacatgat aaaggtacct gtatagcccg tatggtctag ccaatctcct
     3901 tccaaattcc atgctaaaga acgttccttg tcatccaaag gagaaaaatt ttgatttaag
     3961 tctcttgcaa aatcatctgc taaatagtgt tctaaaaaga tttgtaaatc ctttatagtc
     4021 gaaaataaac cagcactccc agcatgtcta cccaggagac gagccttggg atcatgcact
     4081 atgcctgcct ctacacctct aactgttgga acagcaagct caactggccc aaacttggtt
     4141 tccgtcattc cccaaggttt ccagacttga tcctttaaaa tcacatccaa atcttgatta
     4201 aaaattcttt ccaaaataaa gcccaacagc aaaaaatgga catccgaata aagaaaggct
     4261 ggctgacttc gtctgttgag atgaaacatc gcttccttta attcaggggc tgttaaaaga
     4321 tcacgattag gaataaaagg atcaaggtct gttgcatgag tcaagagctg gcgaatagtg
     4381 atgtctggat aatcactctc aggtaaaaaa tctattacca gtctatcaat atctaattga
     4441 cctatttccc acaagaaggt acaaactgtg ccaaccccaa caaccttgct gacactagct
     4501 aggtcataaa ctagtcctgc ctcagtctgc aagccatgct ctgggtcact ctggcctaaa
     4561 tagaactctg tccattgatt gtccttaaaa tacgcaaaag aggctccggg ataaattcct
     4621 gcctcgattt gttcttctat ttttttaata atcttggtcc acttcatact tcttcaaacc
     4681 acaattcaac attatttcta tctttacaaa ggaagaattt ttcagactta ggaataaaat
     4741 attcagtaga ctcaaacttc tggcgaagac ttgctatgtc taattcattg accaagaact
     4801 tgagcataga taagtcccag gtaaccgtat tgtccacagt caaatcctgc ccctgagctg
     4861 gaataaaatc aagggatgcc ccaatttcag atgattccaa gaaactttcg atatcagttg
     4921 ggagatgtaa ttccatagaa atctcaaatt tacttaaaga aattgatgcc aaatctgttt
     4981 gaaattcagg cttttctcct acttctacta gacttgctat gtcatcttcc gcatgaatca
     5041 aaatcaaatc atcttctggt gagaaaattt caaaagcgta gccattttga cctttatata
     5101 atcgatgaat cgaatctgtt ttagataaga ttccttcaat ttctaaggga ttttccacct
     5161 tgacaatcaa tctagctagt ttttttcttc cctctacctt acgagtacgc atactgggag
     5221 cttcttctaa aaccagcttt tcaagacccg tttggtcacc tagtgacaga aaggccgatt
     5281 cttctaacaa ggccttcatt ccaagggttt caatataaaa tgtttcattt aattttctat
     5341 tattagcttt taaagtagga ataatccgta caatctgatt tacattcata aattcctccg
     5401 tcacttttta ttttaaagga ttttagaatt ttttacaaga tattatgctc aaatttaaca
     5461 ttatttagct atttagtaaa gatagtcttt caaggatttt gacaaagcct gataaccagc
     5521 aatactgagg tgcagtccat cagttgtata ttcttttttg agttggcctg cttggtctgt
     5581 caaacaatca aatactggca caaattccac ctgcatatag gcagatgcaa gctcttgata
     5641 ggcttgattc cagttctgaa ttttttcatt cgagcggata tagactgcct gctggtactc
     5701 ctctctctca ttgacaggca aaatggaaag caatttaatc tctgtcaatg gataatcgcg
     5761 agcaacggat tgaatgatag cttcgagatt attgagagcc tcattcacag gaacatcctt
     5821 tccgatatca tttgtcccaa tcagaagaaa aattttatct actgctccac catatagatg
     5881 agcatcaagg ttctctagta acagtcctgt ctgatagcca cgaattcctc gattgacaat
     5941 cgtctttgaa gtcccaaata gctcctgtag aggataatac tcgacaatgg aatccccaat
     6001 aaaaagaatg tttggttcta caacagaaat gtgatttagg tgacgatact tagtttgaat
     6061 tttttcttgt tcctttagga gccaattttc taataactgt actgccactt cattctcctt
     6121 tttctaacca gttttccaag acttgataaa ctcctccttg gctgttggct ggtgctagag
     6181 cagtcgccac agctttggct ttctcatcag cattttccat cgcataggca attccagcca
     6241 tttcaagcat ttcaacatca ttttcactat caccaaaagc catgatttct tgggatttca
     6301 agtcccagcg cttgagtaat tcctccaagc cccatgcttt atgaatccca gcttggagga
     6361 tatcaatgca accatagcca ctggatacag ctcggacacg gccatcaaag agagcattga
     6421 tttcttccaa aaccgaactc aaacgttcct caccaacaac catgctcatc ttgagcacac
     6481 caccaaagag gtcagatgtt aattcatcca caaattgcat ccgttggtag aatttttcaa
     6541 tcatttctgg agtcataaaa ctttcaagat ctgtaaaaat cgtaccttcc ttgacaaaat
     6601 cacccttcat ccccgttaca acaaactggt cctgacacgc tcgacccttg aaatgagtca
     6661 aagccttgtt gacaatggcg tcatcccatg tctgagcctg aatcaattca ttgttttcaa
     6721 aaatacgagc gccattagca acaaccagaa ccactcgatc caccaaggga ctcagtagtt
     6781 gtctcatgcg gtgaatttca ttgcccgtcg caatgacaaa acgaatgccc ctttgatcca
     6841 actgatctaa aatcttttcc aatcgtggga gatcaagctg acctctagca tccagcaagg
     6901 tcccatccat atctgtcgca attaccttaa tcatcattta ctttcctctg gattcaagct
     6961 aataccactt caatcccaca tgttcattca tttctttata ggctgtatca attcgttcct
     7021 tagtcgcttc atctaactgg tcacgatgac tgccatcctc tataaaatct tccaaaatat
     7081 aagtttggta atgatacaag ggatatcctt ctggtgaaaa ggttcccttt ggtgttcgat
     7141 tgacccaagt aggatgagct ttagctgttc cgatagttgt ttttccatcc ttcttcttga
     7201 tggtgacatc catgagaaca ccacgttcag tccacttagc attctcttca tctcccatag
     7261 attcaattcg ttgattggaa atgaagttcc ccatttaata gacaatgagc tttttatctc
     7321 catctttttc caccgtttca gatggttcaa caacgtgagg atgccctcca aagataatat
     7381 ccgctcccca atcaatcatc ttgtgataaa gagctttttg ttcttcagtt ggttccaatc
     7441 gatactcaac acccatctga ggcatgataa tggtgatatc tgcttccttc tctgcccgtt
     7501 caacttcagc ctttatctta tcttcgttta aatctgaaag ataacgatta tagtcttcct
     7561 gagaaatata ctgctcaatt ccattgaaac cataggaata ggctaacaat gcaaccttga
     7621 tagcattcac ttccttaatg accagcggag cctgagcacg tggttcgtgc gtataaactc
     7681 cgattggagt gattccagct ttctcaataa tatcggccat tgaaataact ccctcaattt
     7741 gcgaatccaa aatatgatta tgagctaaat ccagcacatg ataacctgcc tccttaatag
     7801 catccataac ttcaacagga gcattaaaga gaagataacc cgctaaataa tgatccttat
     7861 taatggttcc ttcaaaatca ccaatagcta aatctgcttg cttgagccaa ggagtcacat
     7921 actcaaaatt ttcatgaaag tcataggtac cgtcttcttt tttagctgaa aagaaaagtc
     7981 catcgtggta gagcaaatcc ccgtgggcca taattctggc agttttttcc tcttcctact
     8041 cctgagactt ttgcttagtc ccctcttgag aaatagtatc tcgtttttga ctagtcaagg
     8101 gattccttgg aagctttcaa acaacaagac caagcccatt gataaaccaa ctgctagcaa
     8161 gagtatcgcc acaaatccct tattgctcca cttgcgataa ctcctaaaaa gtttaccaag
     8221 cccttcataa aacgaaaagc tgaaccaccc tgatttcgat tttgtcttct ttgtatcttc
     8281 gttctcccta ctttcttatg caagcctttt ctttttatta tatcacagat aagtatttct
     8341 ttcacaattg aattgaactt cctatctatt ttctataaat cctaaatgcc ataatgcttt
     8401 caattcttgt cattttgtga tatcatgtag aggaaatgaa ctaatccaca gtggcttatt
     8461 ccaagtatac cacttgggct ttggcagtag ctaactgcac taaacataat ataaggagag
     8521 aaaatgtctg caatagaacg tattacaaaa gctgctcact taattgatat gaacgatatt
     8581 atccgtgaag ggaatcctac tctacgcacg gttgctgagg aagtcacttt ccccctatct
     8641 gaccaggaaa tcatcctagg cgaaaagatg atgcaattcc ttaaacattc ccaagatcct
     8701 gtcatggctg aaaaaatggg actccgcggt ggtgttggac tggctgctcc ccagttagat
     8761 atctcaaaac gcattatcgc tgttttggta cctaatattg ttgaagaagg cgaaactcca
     8821 caggaagcct acgatttgga agccattatg tacaatccaa aaatcgtctc tcactctgtt
     8881 caagatgctg ctcttggcga aggagaaggt tgcctgtctg ttgaccgtaa cgtgcctggc
     8941 tatgttgttc gccatgcccg cgttactgtt gactactttg acaaagatgg agaaaaacac
     9001 cgtatcaaac tcaaaggcta caactccatt gttgttcagc atgaaattga ccacattaac
     9061 ggtatcatgt tttacgatcg catcaatgaa aaagacccat ttgcagttaa agatggttta
     9121 ctgattcttg aataaagaaa atcccgttgc aagacggggt tttgtgttat aatagaggca
     9181 tgaaaacaaa tgatattgtc tatggtgtcc acgctgttac cgaagccctc cttgcaaata
     9241 caggaaacaa actctacctc caagaagatc tccgaggtaa gaatgttgag aaagtcaagg
     9301 aactagctac agaaaagaag gtgtccattt cttggacatc aaaaaaatct ctctctgaga
     9361 ttactgaagg tgctgttcat caaggttttg ttctacgagt gtctgaattt gcctatagcg
     9421 agctagatta catccttgca aaaacacgcc aagaagaaaa tccacttcta ttgattctag
     9481 atggtctaac cgatccccat aatctgggtt ctatcttgcg aacagccgat gcgaccaatg
     9541 tttcaggtgt catcattccc aagcaccgta ctgtcggagt aactcctgtc gttgccaaaa
     9601 cagccacagg tgctattgaa cacgttccaa ttgcccgagt gaccaacctc agtcaaacct
     9661 tagataaact taaggatgaa ggtttctgga cctttggaac ggatatgaac ggtactcctt
     9721 gccacaagtg gaatacaaaa gggaaaatcg ccctcatcat tggaaatgaa ggaaaaggta
     9781 tctctagcaa catcaaaaaa caggtcgatg aaatgattac cattccgatg aatggacatg
     9841 ttcaaagcct taatgccagt gttgctgcgg ccattctcat gtacgaagtt ttccgaaata
     9901 gactataaaa aagtttccag tcatctgatt ggaaactttt ttatgattaa ctatgttctg
     9961 taatgaattt ataggcttct tgaccagcga tagctccatc tccaaccgct gttgttactt
    10021 ggcgaaggtc tttcaagcga acatctccaa ctgcaaagat accgtcgact gcagttttca
    10081 tgtggttatc tgtcacaatc catcctgcct gatcttggat attcaattct ttaacaaaat
    10141 cgctaagagg gtccaaacca acatagataa agacaccacc gaaggcttgt tctgtcactt
    10201 gacctgtttt cacattttca aatacgactg attctactcg gttttcaccc ttgatttccc
    10261 ttactacaga atcccagata aagctgattt tttcattcgc aaaggcgcga tcttgtaaaa
    10321 ccttttgggc acgaagttgg tcacgacggt gaacaatggt aacagtctta gcaaaacgag
    10381 tcaagaagag ggcttcttca acagctgaat ctccaccacc aactaccaat aaatcttggt
    10441 cacggaagaa agcaccatca cacacagcac agtaagaaac accacgactg ttcagttctt
    10501 cttctccagg cactcccaaa ggacggtgtt tagaaccagt tgctacgata actgtacgtg
    10561 tttcatatgt ttggtcatca gtcatcactt tcttaaaatc accatggtct tcgacatttt
    10621 caacataacc ataaatgtgc tcaacaccaa gattttcaag tggttcaaac atcttttcag
    10681 ccaattcagg tccactaata ttagcgtatc ctgggtaatt ttcgatatca gatgtattat
    10741 tcatctgacc acctggcaga ccaccttcaa tcaaagctac ttttagattg cttcgagcag
    10801 catacaaggc cgcagtcatc cctgcaggtc cagcaccgat aataatagta tcgtacatat
    10861 agattccttc tttcttgttg taactatctt tattctaact ctttcttgtc aatcacgcaa
    10921 ggattatgcc ttgaaaacaa gaattaaact cataaccgca aaggataata ctgggacaac
    10981 caagactcca atcataatca tatcatagag atgggcttgg cgagccctgg ctgtcttatc
    11041 aactcccgac atggctctca gaccaatcca aatccctaaa aaaatcagaa caaggatggt
    11101 ggtcaagatc aaactctcga aatataaaga aaatagttgc aatagcatga tttctctcat
    11161 ttctatcttt tttaaagagt aaactcagct agtccaacta actgagtttt cctttatcta
    11221 ttatatcaaa tataagtccg tttgtaacta gcgaagaatt cttttgtccg ctcttcttta
    11281 ggggtgtgga taatctcatc cggagttcca gactcgatga ttttcccctt atctaagaag
    11341 agaattttat ccgcaacttg ggctacaaag gacatgtcat gactgaccaa aatcatggtc
    11401 tgacctgact tagcagcatc tgcaatagac ttttctactt caccgaccaa ttctgggtca
    11461 agggctgaag ttggttcgtc taagagcaaa acatctggtt tcatagcaag cgcacgcgct
    11521 agggcaaccc gttgcttctg tccacctgat aaatggcgag gataatggtt ttcacggtcc
    11581 gaaagcccaa ccttagccaa ctcttccttg gcaatcttag tcgcttcttg gtcagataat
    11641 ttcttgacaa caaccaagcc ttctttcaca ttatcaagtg ctgttcggcg ttcaaataaa
    11701 ttaaactgtt ggaaaaccat agacaactta cggcgtaggg caaggatttc ttcttgagtg
    11761 attttagaaa aatcaactga aaaaccatca atctgaatag agccactgtc aggtgtttca
    11821 agataattga gactgcgaag aaaggttgat tttccagctc ctgaagaacc aatcaaggct
    11881 acgacttctc ctttttgaat atccaagttc agatgatcca agacagtctg tcctgaaaag
    11941 gatttgctta aattcgaaat cttaatcatt aacgaaggtc tcctttcaca tctgtttgca
    12001 ctgtatcagg tgcagaaata gccatttttc tctcgatgaa acgaccgagg ctttcaattc
    12061 cgatattgac tacccaataa acaagggcaa cggagatgaa gcgttcaaaa tagcgataat
    12121 cagctccacc tagaatctga gcttgggcaa agacttccac aacacccgca ctaaaagcta
    12181 gagatgttcc cttggtcaaa ccgatgaggg aattaatcaa ggttggagta gctaccaccg
    12241 ctgcattagg aataatcact cgtcgataaa cttgcgctcg ggtcataccc agactgcgtg
    12301 ccgcctcaat ctcaccagga ttaactgaga gaatggctgc acgaatggtt tcactagcat
    12361 aagctgcctc attaaaggca aaagcgacaa tcgcaaaagc tgcagctgga atcgcattga
    12421 tattgagacc agttccccat tgctgattga gggctttcaa agccaaaggg attccgtagt
    12481 aggtcaacat gagttgcacc aaaatcggtg tcccttttaa gaaactaaca aagaaggcct
    12541 gcaagggata taaaatcttg acacgattga tcttcacaat ggcaaaaaga agcgccaaaa
    12601 ccaagccaaa aagggcacca ccaattgtca acataattgt tgttggaagt tgttggacaa
    12661 ttctagggat tccatcaaag accgaacgta ggctaaacag cttgccatcc ggaatcaatt
    12721 gcatcaagtt ttggtaccaa tctgatgcta aaatcgttgt aacattcata aaaacatcct
    12781 cttctgataa tgattcattc taccatgctt ctgccgtcaa tgaaattatt tgctacggtc
    12841 agatacagac tttgtctacc tactagttta tcatactttg aaacagggaa gttatatatt
    12901 ttatctatca aagagataga taaaaactat ttcagtccca gttcttcata agattttcga
    12961 taaccgattt gcttaacagt tccattttct actaaaatgg gccgttttaa caacataccg
    13021 tcacttgcta gcaactcagc cgcttcttgg tttgacaaac ttcctacctt atcttttagc
    13081 cctaattcac ggtatttgat accactggtg ttgaaaaatt gcttcaattc aaatcctgag
    13141 gtttctagcc aattcaaaat gacttcttgg ctaggtgttt cttccacgat atggacggct
    13201 ttatagtcca cacctaattg atttaattct tgttttgctt ttttacaagt tgaacatttg
    13261 gggtattcga taaattctaa catgtgcttc tccttctatt tgtttatatc tttaaaatct
    13321 acgccttcat gctccaagag ccaagctttc ttttccactc ctgcagcata gcctgtcaga
    13381 cgcttgcctg ctcccaacac acgatgacaa ggtactagga tagaccaagg attgcgtccc
    13441 actgctccac caattgcttg agcagaagcc acttgcaggt cttgagcaat ttgtccatag
    13501 gtcactgtct gaccataagg aatgccctgt aaatagtccc aaactctctt ttcaaaattc
    13561 gttccgattg gcgccaagag caagttggat aaatcctgag gcttgccttt aaagtaatca
    13621 tctaagcaag caataactgg gtctaaaata ggatgactaa caacttcttc tatcgtttca
    13681 tctcctagtc ccctctcaaa atgcttctgc tcctgaaccc aaattccata caaataatgg
    13741 tcatcagcta caagtgatag aataccaatt ggtgaggagt agagtatttt tacatacttc
    13801 ttttgcatta tttctttaat ttttgatagg ccaagcgttc gccatctact ggcaccttac
    13861 caacttgttt aaaaccaagt ttttcaaaaa tatgttgcat aaccttgttt tcagcatgcg
    13921 tatctgagcg aaaatcaaga taatcaaaac cttcaatcaa gccctctaag aaggtttgag
    13981 caactccttt tccctgcaca tctgctgcca cagcaatacg gtgaaagact agatactctg
    14041 actctccagc ttgccagttt ccctcataaa tagcttcata ggcctcctct ggactcttgg
    14101 tcacagcagc ataggctagc agttctccct cttccaaggc tacgtaggct tgccctgaga
    14161 tgatatcatc gataataaca tctgcatttg gatagccatt ttgccactgg tcactaccag
    14221 catctgctaa acattttttg gcatcctcca tcacctgcat gatggcatct acttcatttg
    14281 gaaaagctaa acgaatttcc atcttttttc ctcatttctg actagtttct cccatcatag
    14341 cataatttaa gtatttctac aagcagttaa aacttgactt tgtgtttttg ttattttata
    14401 atctagttat caaaactata taaaaaggag ttaaaaatga aaactacctt ttcctaccca
    14461 aaatgggcag aaattccaaa cattgacctc tatctggacc aggttttgct ctatgtcaat
    14521 caggtctgcg cccctatctc tcctaataaa gacaagggcc taacagcatc tatggtcaac
    14581 aattatgtga aaaatggtta cctgacaaag cctgacaaaa aaaaatacca acgccaacag
    14641 attgcccgtt tgattgctat cacaaccctc aagtctgtat tttctattca agaaatagcc
    14701 cagacactta atactctaca aactcaagca agttcagacc aactctacga tgcttttgtg
    14761 gactacatga accaaggaat tgacccagct aaccctatta tccaaaccag ctgccaaacc
    14821 gttaaactct atcatcaaac tctagactta atcgatcata ctcaagagga ggtaatccaa
    14881 tgaacactag tcttaaactc agcaaacaac tcagttttgg agaggagatt gctaatagcg
    14941 tgacccatgc tgtgggtgca gtcatcatgc ttatcttgct gcctatttca tccatctata
    15001 gttatgaagc acacggattt ttatcctcta tcggcgtttc cattttcgtc atcagtctct
    15061 ttctcatgtt cctatcatcc accatttatc actctatggc ctatggttcg acccacaaat
    15121 atgttttgcg aatcattgac cattctatga tttacgttgc cattgccgga tcatacacgc
    15181 ccgttgtctt gaccttgatg aataactggt ttggctatct gattattgtc atccaatggg
    15241 gaacgaccat ctttggtatt ctctataaaa tctttgctaa aaaggtcaat gagaaattta
    15301 gccttgctct ttacctgatt atgggctggt tggttctggc tatcattcct gccattatca
    15361 gtcaaacgac acccgttttc tggagtctca tggtaactgg cggactctgt tatacagttg
    15421 gagctggatt ttatgccaag aaaaaacctt atttccacat gatttggcat ctctttatcc
    15481 tagctgcgtc cgcacttcaa tacatcgcta ttgtttatta catgtaaaaa agttgagaaa
    15541 ttcaatctca acttttttct ttacacatat tgataaagta ctggtgcaag cgcacatcat
    15601 cagtcaattc tggatgaaaa gaacttacca acatattttt ttcttgggct gcaacaattt
    15661 gattgttcac tgttgctaaa atttctacac cctcaccaac actactgata atcggaccac
    15721 ggataaaggt cattggaatc ttgccaactc ccttacattc tgcttccgtg tagaaacttc
    15781 ctaattggcg cccataagca ttacgctcga ccaccatatc catagttcct agatgactct
    15841 ctttctgaga agtgatttcc ttagccagca aaattaagcc cgcacaggtc ccaaacactg
    15901 gtaagccaga tagaatggct tctcggatgg gaagtagcat gttctggtca cgtaagagct
    15961 tgcccatggt tgtagactca ccaccaggca aaatcaaacc cgacaagtca ctctgatctt
    16021 gctgaaaatc atctagattt ctgagttcta cactctcgac acctaattga tctagcactt
    16081 ttgcatgttc tgcaaaggcc ccttgcaagg ccaatattcc gattttcatc tattttcctc
    16141 gttcagccat gagaatttgg atttcatttt cattaatacc aaccatggct tctcctaaat
    16201 cttcagagat ttgagctagg atttgaggat tacggaagtt agtcacagcc ttaacaatgg
    16261 cactcgctcg tttaacagga tctcctgact tgaaaatacc tgaaccgaca aagaccccct
    16321 ctgcccctaa ttgcatcatt aacgcagcat ctgctggcgt tgcaacacct ccagcagcga
    16381 aatttacaac tggcaatttt ccatgttcat gaacatattg gaccaattct acagggactt
    16441 gcaaatcctt ggcagcaaca taaagctcgt cctcacgtaa gttttgaatg cggcgaattt
    16501 cctgattcat catacgcata tgacgaacag cttggacgat atcccctgtc cctggttctc
    16561 ctttggtacg aatcatggaa gcaccttcag cgatacgacg caaggcttca cccaaatcct
    16621 tagcaccaca gacaaaagga acttggaatt ctttcttgtc cacatggaaa cggtcatcag
    16681 ctggagatag aacttcactc tcgtcgatat aatcaatttc aatagcctct aaaatctgag
    16741 cttcaacaaa atgcccgatt ctgaccttag ccattactgg aatactaacc gcttcttgga
    16801 tttccttaat catctttggg tcgctcatgc gggaaactcc tccagctgca cgaatatcag
    16861 ccggaattcg ttccaaggcc atcacagctg ccgcaccagc agcttctgcg atacgagcct
    16921 gttcaggatt ctgcacatcc ataataacac cacccttgag catctgtgcc aagtttttat
    16981 ttagttcata acgattttca gtcatttgtt ttatcctcat catttgtatt ggtagctacc
    17041 ttttcatttt aagtcagaac agaatgaagc acaagataaa aaaactataa ttgtatgggg
    17101 acaaattagc taaaaactat ctgaccttaa cttaaggcca gatagctcat tcatttttat
    17161 ttttcagccg taagggcagc cattgtgatg tagttgtatg gtttgttgaa gtgtggcaag
    17221 aagaagaggt ctgtcaatgc caatttatca attgtcacat gctcttggat agcaagtgag
    17281 aacatgtgga ttcccatgct aattgcaata tcatgtgaaa ccatttgggc accaagaatt
    17341 tcacggctat ctttgtcaaa gacaatctta attgctactt catggttgtc atgtttcatg
    17401 aattctggtt tttgaagatc gttaaagcct gtttcagttg cgttgtaacc agcagctttc
    17461 gctttttcaa gagtcaaacc agttgaaacc atgtgaagac cgtagattga gataccattt
    17521 gaaccttgaa caccgattcc ttccaattca tgtccacaag cattgtaggc accaacgata
    17581 ccagtgcgaa cagcatttga agcaagagcg atatagcttg tatctttacg agcattgtca
    17641 taaacagtcg cacagtcacc aacagcgtaa acgtctggga tagatgtttc ttgtttcttg
    17701 tctacaagga aggcaccgtt gcggaagagt tcgatcttac cacctgcaag ggctgtgttt
    17761 ggacggaaac caactgcaag gataaccata tccacgtcaa agctttcttt gtcagtaatc
    17821 aagcgttcaa ctttaccgtc accttcgatt gctttaacag tttgacctag agccaagcgg
    17881 atgttgtgat cttctaagtt cttcgccatc atttgtgtga agtctttgtc atagtaaccg
    17941 ttcaagacag tatcaacgat atcaacaagg acaacttctt ttccaagacg ttcaaaggct
    18001 tcagcaagtt caacaccgat gtaaccacca ccaacaacgg cgatacggtc gaggtgttgg
    18061 ctcttgtcag aaagtttatt gataacttct tcagcatttt ggtacaattt cacgaattgt
    18121 acgttttcaa gagttgcttt aaattcgcgg tttcctttaa caatttcaac accttcgatt
    18181 ggtggcaaga ttggtgtaga acctgtagcg aaaatcaatt tttcgtatga ttctttgtgc
    18241 tcttttcctt caacttccgc tgtaactact ttgttatcat agtcgattga aagaacaggt
    18301 gagttcatgt aaactttagc acctttagct tccaattttt ctttatcaga atagaacaag
    18361 ccttcagcac cgtcaatttg ttcaccaatc caaagagcca ttccacatcc taggaaagag
    18421 atgttagagt tttggtcaaa tacaacaatt tcgttctcat ttccaaaatt atccaacatg
    18481 gtattgatac atgctgtacc agcgtggtta gcaccgacta caacgatttt actcataaaa
    18541 atttcctgcc ttcattctaa atttacctta ctagtatacc atttaccgtt agcgtttaca
    18601 aggggggtac tattatttca gcatttttta tcaaaaataa ttccccattt atcctgtttt
    18661 caagcctgat gaattttatg agcatatttc ttgacttttt ccaaattttg tttgtgaaaa
    18721 ttctaacttt atggtattat aataacatga aaatgaaatg taagggcttc aatttagatg
    18781 attatttgat attacaactt attttctata agagaaagga gttggtagct tgactcttcg
    18841 ttcacattct gaacggctaa tggggactac tatcactatt tcattagtgg atgagcaagc
    18901 cgatatcttt ctccaaaaat cctttgactt gctcaaagaa cttgaatacc gcttcaacgc
    18961 caatagtcaa gaatctgagt tgatggaaat caattatcaa gcaggaatag cccccgtcac
    19021 ggttcatcca gacctgtttg agctgatttc acttgggtta gagcatagcc tagcgctctc
    19081 tagccacctc aatatcagca ttggtccctt aattcaaacc tggcgtatcg gtttttcaga
    19141 tgccaaggtc gcccagcctc aagaaattga atcggtgctg cctctaatca atcctcatgg
    19201 tatcgagtta gattcttcta cttctactgt gtttttaaaa cagaaaggaa tgaagatcga
    19261 tctaggttgt ttagccaaag gatacagtgc ggataaggtt gcccaatttc ttaggaaaga
    19321 gggggtgact tctgccttga tcaatctggg agggaatatc ctgaccattg gaaaaaatca
    19381 ggcaagaggg gataacccat ggcaaatcgg gattcaagac ccagccaatc ctaggggaaa
    19441 tcatttaatg accatccctg ttgtcaataa atctgtcgtg acttcaggca tttatgaacg
    19501 tcacctgacc gtcgatggac aagattacca tcacattttt gacagtcaaa caggatatcc
    19561 tgttgaaacg gaactagcga gtctaacaat catctctgat aaatcagtcg atggcgaaat
    19621 ctggacgact cgactctttg gagaaagacc tgcttctatc ctctggcaag tcgaaagttt
    19681 ggagggcatc gaagtcatcc tcatcgataa agaaggccac ctaagctgtt cttcaggaat
    19741 tcctactcta tagagacaac tgtttcaatg gaattttaaa attgtattat gtaaaaagag
    19801 aaaggaaatc tcatgttaaa acttattgct attgttggaa caaattcaaa acgttctaca
    19861 aaccgtcaat tgcttcaata catgcaaaaa cactttactg acaaagctga aattgaactt
    19921 gttgaaatca aggccattcc tgtcttcaac aaaccagctg acaagcaagt acctgctgaa
    19981 atattggaaa ttgctgctaa aatcgaagag gcagatggcg ttattatcgg tactcctgag
    20041 tatgatcact ctattccagc tgttttgatg agcgctcttg cttggttgtc ttatggtatt
    20101 tacccacttt tgaacaaacc aatcatgatt acaggtgctt cttacggtac gcttggttca
    20161 tctcgtgccc aattgcaact tcgtcaaatc ttgaatgctc ctgaaatcaa ggcaaatgtt
    20221 cttccagatg aattcttgct ctcacactct cttcaagcat ttaacccaag tggcgacttg
    20281 gttgaccttg atgttatcaa gaaattggat gccatctttg atgacttccg tatctttgta
    20341 aaaatcacag aaaaattacg taatgcacaa gaattacttc gcaaagatgc tgaagacttt
    20401 gactgggaaa atttgtaaga taggagacca aaaagaatga aatttgttgg acttgttgga
    20461 tcaaactacg atcaatcata taaccgtaag ctcttggaat ttatccgtcg ctacttcaaa
    20521 ctcaaatttg aattagaagt tcttgaaatt gacgaagttc caatgtttaa ccaagatgaa
    20581 aaatgggatg aaagtttcca attacgttat ttatataaca aaattactcg tgcagatggt
    20641 gttatcattg ctactcctga gcataaccac actatctcag cttcactcaa atctgtactt
    20701 gaatggcttt catacgaagt tcatccattt gaaaacaagc ctgttatgat tgtgggtgca
    20761 tcatactatg accaaggaac atcacgtgct caagttcacc ttcgtaaaat ccttgatgct
    20821 ccaggtgtta atgcctacac gcttccaggt aacgaattcc ttcttggtaa agctaaggaa
    20881 gcttttgata acaacggaaa tatcaccaac gaaggcactg ttaaattcct tgaaacttgc
    20941 ttagataact ttgttaaata cgtaggagtc gtttcgaaat tgaaaaaacc aaaaccaatc
    21001 gaatcagaag acttggattg tggaaaacca atcgctacaa ccatcacaga agttgaccct
    21061 gacgatccag aatgggtaga aaaagttgca gcattcactg gtgctgtttc tggtgatacc
    21121 tatgtcaaat tggaccacgg tatcctaaca gttaaccaaa ttgatatgtt cttgaaagct
    21181 atgccatttg aattgacata cgctgacgac aacaaccaat tcctctacta caacaacgct
    21241 caccaagatc cagacactat gttcgctaaa cgtgtaccac ctcaatcagg tagccgtatg
    21301 tcgactgttc atggttctct tccaccagca cgtatgaaga acgtagagtg ggttatcgga
    21361 acacttcgca acggaaacca agaatacgtc cgtacgatcg ttccaggttc tcctgcaggt
    21421 gttatcaaca ctcacaacta ccaagcaatg tactatcctg atggatcata cgctggtatc
    21481 aatgaaatcg tctttaactt ccaaccatgg cttgactggt acctaaaaga gactggtcaa
    21541 cgtttggtgg gtggtagcgg accatttgct cctgctgccg gaggtcatgg agacgccgat
    21601 gctacttcta gcgcttctga ttcaggggat gctggaggcc acggtggtga cgcagacgct
    21661 acagccggtg caagttacta atgatagttt ttaggaacta ttatggttcc ttttttagcg
    21721 aaaaaagact agcaaacaca aactgctagt ctctttgtta tcgtttcatc tatctttgtt
    21781 aaaagaaaga atacatatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag
    21841 gctgtactta agtacggcaa ggtgaagctg acgtggtttg aatttgattt tcgaagagta
    21901 ttattatgaa tttggatcat caagactacg gccatgtaat cctttttcac gttgtacttg
    21961 gcgtagtttt tctggtgtaa catcgtttcc ttcttcgtcc acaattttga ttccttcaat
    22021 gtggtgacga acagcgcggc gataaccttc gatgtactcc tcacgtagtt tggcttgttc
    22081 cactttttct tctggtgtta agccttctgt ttttttcttt ttagcaagct cattgatacg
    22141 agcaattttt ttcggatcca tttcttctcc tttgtaataa gataaagtcc gtttatcaaa
    22201 ttttatttag tgttaatttg gttaaaacaa gcaaacttag ctgctttctt ggttagttgt
    22261 gacaagattg ccaaacgatt ttgacggaca gcctgatctt cagccattac catagtattt
    22321 tcaaagaaag catcaatgac tgggctaagc gcaaaaagtt gtttcaattg ctgacttgca
    22381 ggtcctgata aaatgagtgt ttctactgct tctgccaaag ctttttcttg gtcattctca
    22441 aatagtgctg aatcaaccgt agcaacccct tctgccttct cggccaggtt aaaggcacga
    22501 gaaagtgatt caacagatgg tttaaaatct tcttccttgc ttacttctac gagagcactt
    22561 gctgcttcca acatatctgc cacaacaaag tttgaacctg caagaactgc ttccttgata
    22621 tcttttggag tagagcccat catcttatca acacgagcct tgataaagtc cataacctct
    22681 gctttatttt cataagtcaa actgtcaaat ttcaatgcat aaaggctatc aatcagctca
    22741 tccatagcaa tgtgccaacc aaaggcatcc aagatacgaa ccacaccttg agttgcacga
    22801 cgaagggcat aagggtcatt agaacctgat ggaatcaatc ctactgagaa gaaactcaaa
    22861 atcgtatcca atttgtctgc aatggctaga actgcgccga ccttgctctc tggaagttct
    22921 ccttcagctg atgtaggcat gtagtgttca cgaatagcag ctgccaccgc tggagtttca
    22981 ccagcaagaa gggtgtattt ttcacccata attccttgga gttcgtcaaa ttcaccaacc
    23041 atacctgtca acaagtcaaa cttgtaaatg gctgctgcac gagcaaggtc aactgtttca
    23101 tccactgaca aactagcttt ttctgccaaa agtacagtga tttgacccgt acgaatcatg
    23161 tgttcacgaa gggaaccaat cttctcatgg aaggtgacat tgtttaattt ttcaacaaga
    23221 tctgaaatca ccaatttttg gtcttcacgc cagaagaatt ctccgtcttc caagcgggct
    23281 accaagactt tttcatttcc tttgatgaca tttttcaaac gctctgcgtt tccgttacga
    23341 acagaaatga agtttggcaa gagttttcca tcttgatcac gaacaacaaa gtaacgctgg
    23401 tgttccttca tagaagtcac caagacttct tctggaactt caaggtattt agcatcaaaa
    23461 cttcccatga aggcagttgg gtattcaacc aaattcaaga cttcattcag caaatccgca
    23521 tcaatttcga tacgtacacc atgttttgcc tcaatttcct tgatttggtc aacaatcatt
    23581 tgctcacgtt cacatggatc tgcgattaca aactgcttac gaaggtcttc ttcatagctc
    23641 aatgctgact gaatcttggt ttcttgtccc aaaaaacgat ggccacgact cacacgactt
    23701 cccttgatat caaggaaatc caagtcaaac tcttgctcat ccaagagaac agttaaagtg
    23761 tgaacagggc ggatgtattc aaagctattt cccgcccagt gcatgctgac agggaaagtc
    23821 agtgacttca agacatccac aatgcctgga acaatggctt caactgcttg accaatttct
    23881 tccttagtga catagacata ttcttcaccc ttgatttcac ggaattcgat atcttcaaca
    23941 gtcaaacctt tcccacggac aaatccttga gctgctttgg tgaagtttcc atcactatct
    24001 aaggcaattt tctttgctgg acccttgaaa tcttctgtta aatcagactg tttgtctgca
    24061 agaccagtta cacgaacagc caaacgacgt ggtgttgaga aagtttgaat ggcttcaaaa
    24121 gacaggcgtt ttcccttgag gaaggctgcc attttttcgc ctagttgttt ttcacttggc
    24181 gtaacaacat aggctggtaa ttcttcaaga ccgagttcta ctaataagtt ttttgtcatg
    24241 ttttttcctt tacattttct tcaatctttt ttgatatgca ccttaggtac tggggacgtc
    24301 gctaactaac tctgtgagtc tgtatggaaa tccaatgcta aatttatcaa gatttccaac
    24361 aatctcatca aagtggattt agctgactga tttttgaacc taaggtttca aaaatcccca
    24421 cataacagta cagcggtgca tatccctcta gcaagtcttc gacttgcctc taacgatttt
    24481 agagtacagt attactcact attctgcgtc ttctgctaaa agtttaactc gtgtttcttc
    24541 atccaaaagt gggtaaccta ggcgtttgcg ttctgcaaca aaggttttgg ctacaacacg
    24601 ggccaagtta cggatacgag cgatatagcc tgcacgctct gttacagata cggcaccacg
    24661 cgcgtcaagc agattaaagg tatgtgaaca tttgagaaca tagtcatagg cagggtgtac
    24721 caagccttct tctaatgcac gaccagcttc tttttcaaac ttatcaaaat tttcaagcaa
    24781 catttcttgg tccgaaattt caaatgaata ttttgagtgc tcatactcag gctggataaa
    24841 gatttctccg tattttacac catcagccca ctcgatatca tagacagagt ctacttcttg
    24901 aatgtaagaa gccaagcgct ccaaaccata ggtaacttcc gcagtcacag ggctagttgc
    24961 caatccaccg acttgttgga aataagtgaa ctgagtgatt tccattccgt caagccaaac
    25021 ttcccaacca agaccagctg aaccagttga tgggttttcc cagttgtcct caacaaaacg
    25081 aatatcgtgc tccaaaggat tgattcccaa tttttccaaa gactcaaggt aaagttcttg
    25141 gatatttgat ggagaaggct tcatgaccac ctggaattgg tggtgttggt agagacggtt
    25201 agggttttcc ccataacgac cgtcagcagg acgacgtgat ggctctacat aagctgcatt
    25261 ccatggctca ggtccgatag cacgaaggaa agtgtaagga ctcattgttc ccgcaccttt
    25321 ttcattatca taagcctgca taagcataca accttggtca ttccaaaatt gttgcaaagt
    25381 aaggatgatt tcctggaaag tcaatttctt agacattgtt tactccttta gtttttatat
    25441 ttctttttca attaatttct gctgaacaaa ccaatctaac aagggaaaag ttttttctgt
    25501 atttgcccat aaatgataag aatctaagac cgcttgtaca ctacctaaat tttctaccaa
    25561 cttatctact gtcaaaaagc tgcgccagta ttcataaaaa atacttgcat agttcttatc
    25621 ataagtcgaa gaaccaaaat cattcaatga atgccaacca tacttcttct gaaaaagttc
    25681 tacgagagat tgattacaaa ttttttccgc ttgaaattcc tcttctgtca agaaatactt
    25741 gcgactaata tattcaacca tcccctcttc gaaccagata taagagtcat aaccatcaaa
    25801 atcatctaaa aaatgctccg accaatgagc taactcatgt cctacaatct gtaagaggaa
    25861 attttcagat aaagaatgat agtgactttc tattgcttga gtttgctcag aacactcgta
    25921 gtccatcaat tgatacaaat acaactcttt ccaaacagct aaatcaggcg ccataaccat
    25981 tcgtctatta tttgtatagg ctggaacagc gctttcccta ataatctgtg tagcagcatt
    26041 aaaatctgac caaataacgg cttgcggtaa atcacagact gcaagttcat tctttaaaaa
    26101 agattgataa tctttcaact tttcagtatt tcttactaca aaatcacgaa atgtagctag
    26161 ttgactatcg tcttttacaa gataaaggtt gtccacggct ctcctttctt tcacacaata
    26221 gactgaataa gcaaaagaac cacatctgac aatcctaggt tgatttaacc taggggcgtc
    26281 agaaacgcgg ttccacccta atttattact tcattgtttt tgaaaatact acagaaagcg
    26341 ccagctcatt attttgccct acttggctcc cactgtccca agctcgcttg aagaacgcca
    26401 taagactttc tttcctgctg actattatat caaaaattag aataatgtac aattcttttt
    26461 atgattttct agaaatccac atcatcaact cgtggagccc ccgactgaac agcagtcgtt
    26521 tttaaggttt ctctctcctc atgactcagt tcaattccaa aacaatcaag attagcttga
    26581 atacgagagg ttgtgacaga ttttggaagt ggtaaaaatc cttctgccaa gctccaggcc
    26641 aaggctatct gagcaaccga ttttccgtga tttgctgcga tttcttggac ttgcttgcta
    26701 tcaaacagtt ctccttgtcc aaaaggcccc caagcttcca ataaaattcc cttttcacga
    26761 cagtaagcta cgacttcctc ttgatacaca cctggcgcca agcgaacttg attgaccgca
    26821 ggaacgatag ttgcagtttc aagcaaggca tccaaatgat ggggaagaaa attgctaacg
    26881 ccgatagcac ggattttccc ttcttgatag aggtcttcca tcgctctcca aacttccgca
    26941 ttgcgagttt tccatgcgtc attttctctg agcggttttg ggttcggcca atgaatcaaa
    27001 tacaaatcca aataatccaa gcccagtttt tctatagatt tttccaaagc ttgacgagtt
    27061 tgctcatagg tttgctgact attccaaagc ttggtagtta cgaacatttc ttcacgtgga
    27121 actccgctat ctttgattgc ttgaccaaca ctttcttcat tctgataaat cgccgccgta
    27181 tcaatatgac gataaccagc cttcaaggat tctaacactg cacgataggc ttcttctcca
    27241 tccttagcct taaaagttcc aaatcccaat actggaattt ctactccatt atttaattga
    27301 taggtattca tatgcttctc cttctatatt tattcatcaa ttatagcata aaacattttt
    27361 taagtagtac tatcttctgt aaaaaataag tctgtcaaat atctaacaga cctatttttt
    27421 cttgcttatt catcacgact atagtacagc tcatgagctt ttaggcgagt attgagcatc
    27481 tctggtatgg tcacaaaggt ataaccttga tttttcaaat actcaatgac ccttggcaag
    27541 gcattgactg tcggactgtg aatatcatgc atcaaaacga tagagccgtt agctacttga
    27601 tgctgaattt ctgtcaaaat agatgcttca tttttactct tccagtccag actatccaca
    27661 tcccacatga taaagctcaa atccaagcta ttgcgaatat catctgtaat agcaccataa
    27721 ggtggacgca tgagtttaga actagaaccc agcactttag ttagcacatc ctcagtatca
    27781 gtaatctgct ttttagcttc atcaagagag agttgcgaga gaatcggatg gctccaacta
    27841 tggtttccaa caacatgacc ttcagatttt atcctcttca ccaagtcctc attcccagaa
    27901 acatttttcc caagcacaaa gaaagtcgct ttaatatcat atttagctag ggtctctaat
    27961 acctgcgggg tcgttgctgg atttggacca tcatcaaagg ttagagcgac aactttttga
    28021 tatttcttat caaagtaaga ttggtacaag gccgcatctt tttcgagtaa gtacgaagat
    28081 tggataacat caaagaaagc agatactggc aaggctatct cttctaaatt ttcaaccaca
    28141 ggacttggat aaaggataat ctgactatcc ttgtaatcaa aattccatgc agacaagtct
    28201 tggtcagaga agttttttac aatctgctca ctctggtctt gctctatttt tttatcctct
    28261 atgaaggagg tcaactcttt tatcagctgt tccttagcct tactagcatc tgaaaacagt
    28321 tggtcaagtg taaaaggttg cccatcttct gttaagtgaa cctttcctag actggtcttt
    28381 tcagtctctt caaccttaaa agcaactaaa tcatagattt gtttggtcac attacgatta
    28441 acgactccct ttaaacctga ctcttcttgc tctgtatagt agaaaacaag attgtcctta
    28501 ctttctagct tgtccttaac atcttgattt atcaactccc taacagagga aatcactttc
    28561 tccccttgga gaggataata ggcaatcact tcggcttgcc cctgacgaaa atgctccttc
    28621 tgatttccct cactcaattg atcatctttc tcttttttga gcgattcaat cttttgctca
    28681 aaactttttt gctggtagat cttaaaagca ataaatccgc ctaataggca aatagaaatt
    28741 gctaaaatac caatcaaagc caataataca tgtctcgttt tcccgtgtct gccacgtcct
    28801 agtctacttt tattcatatt tatatcataa caaatttgag caataatttc aatgatacaa
    28861 tttggcaatc cacaattcct ctagcaagcc aaagcatctt acatcaactc caacatccgt
    28921 atctgtagag attatgtagt tttctatcta tggaatttct agtagattga agctagaata
    28981 gtagacctat acttctaaaa cattgttaga aatcgatttg attgtcctga tcgatttgtc
    29041 ctgttctcat ttcattttac tataaaacta tgaaaagcta ggtcatctga gaagtaatta
    29101 ctatcgacaa ttgcgatagc gtatactggt gcgatttgtt tgtagctctg gtgggtatca
    29161 ccttcacgtt ggcgaatttt ttctaggttt tgattaacct gactgcacag ataagcccat
    29221 aggcgattga tgaaaaaatt ctgatgatga acctgaattt cgatgataac ttggattcca
    29281 ttatctaact ccgccaagac gtctatacta gtatagaaat cctgcgctga gtagggcatg
    29341 gaaggcaaga cgtgaatgtt acttccctcc aaaatcgtca cattttttgc tggcaagtcc
    29401 agcatatcgc ggataaattg acaaatcatt agttgggctg atacccggat gtctgagaat
    29461 cctccttttc ctccttctat tatagcacat ttttaaatct aggtttgcta gattctatgc
    29521 ttctatctat ttattcggaa aagaaagcta atagcttatt tgaaattttc tcctctctgt
    29581 tggatagtcc cgtctatcta tgttataatg aaagaaggat ttaaaatcgg aaaaggagta
    29641 tttatgctaa aattaggtgt catcggaact ggcgctatca gccatcattt catagaagca
    29701 gcccatacca gtggagaata ccaactggtc gcaatctatt ctagaaaact agaaactgca
    29761 gcaacctttg cttctcgcta tcagaatatc caactattcg atcaattaga agtcttcttc
    29821 aaatcttctt ttgatttagt ctatatcgcc agtccaaact ccttacattt tgctcaggca
    29881 aaagctgcct tgtctgctgg taaacatgtt attctcgaaa agccagctgt cactcaacca
    29941 caagaatggt ttgatttgat tcaaacagct gaaaaaaata actgttttat ctttgaagca
    30001 gctcgtaatt accacgagaa agcctttact actatcaaaa actttttagc agataagcaa
    30061 gtgttaggag cagatttcaa ctatgccaaa tactcttcca agatgcctga cttgttgact
    30121 ggacaaacac caaatgtctt ttcaaaccgt tttgctggtg gagccctcat ggatttgggg
    30181 atttatcccc tctatgctgc tgttcgtctt tttggaaaag ccaatgacgc gacttaccat
    30241 gctcaacagc ttgacaatag cattgaccta aatggagatg gtatcctctt ctacccagac
    30301 tatcaagttc acatcaaggc tggaaaaaac atcacttcca atcttccttg cgagatttat
    30361 acaacagatg gaaccttgac tctcaacacg attgagcata ttcgctcagc tatttttacc
    30421 gaccaccaag gaaatcaagt ccagcttcct atccaacagg ctcctcatac gatgactgag
    30481 gaagtcgctg catttgcaca catgatccag caaccagatc tgaatctcta ccaaacttgg
    30541 ctgtatgatg caggctctgt tcatgagcta ctatatacca tgcgccagac tgctggcatt
    30601 agatttgagg cagaaaaatg aaaaccaaac taccgactga atggcaagaa ctgagtgacc
    30661 aactcggttt ccaagaattc acccccattc aaactcaact atttgagccc cttcttgctg
    30721 gagaaaacct cctaggagtg agccaaaccg gaactggtaa gaccctagcg tatctcctac
    30781 caagtcttct cagactacaa aagaaaaaag cccaacaact cttgattcta gcaccaaaca
    30841 cggaacttgc tggacagatt tttgatgtat gtaaaacgtg ggcagaagct atcggattga
    30901 ctgctcaact cttcctttca ggttcaagtc agaaacgaca aattgaacgc cttaaaaagg
    30961 ggccagaaat tctgattgga actcctggcc gtatctttga actcattaaa cttaaaaaaa
    31021 tcaagatgat gaatgtggaa accatcatcc tggatgaatt tgaccaatta ctagatgatt
    31081 ctcagattca ctttgtagaa aaaatcactc actacgcacc tcgtgaccac caactggtct
    31141 acatgagtgc cacgaccaag tttgaccaag aaaagattgt gccgaataca cgcactatta
    31201 atctctctga ccaaaaattg gacaatatcc agcatttcta catgcaggta gaccaacgtc
    31261 atcgagtgga tatgctacga aaactggctc atgtagagga tttccgtggt ctagtcttct
    31321 tcaatagcct gtcagacctt gggaatgccg aggaaaaact acagtatcgt gatatattga
    31381 ctgtttccct cgctagtgat gttaatgtta aatttagaaa aatcatctta gaaaagttta
    31441 aagataacca actaaccctg cttcttgcaa ctgacctttt ggctcgtgga attgatatcg
    31501 atagcctaga atgtgtcgta aactttgata ttcctagaga tagcgaaacc tacacacacc
    31561 gtgctggccg tactggccgt atgggcaagg aaggatatgt tattactctc gtcactcatc
    31621 cagaagaaat caaaaaactt aaaaagtttg caagtatccg agaaattgtc ctaaaaaacc
    31681 aagaactcta tatcaaataa agccccattg ctctatacac atccagtgta tggagctttt
    31741 ttataagcaa ttcaatttta tactcatctg ctttcaaaaa gcattctatt ccatctccga
    31801 ttaacgatgg actttatcac ctccttctcc agtccttgta tgacatcttg gagttgattc
    31861 atgacatctt ccaaagttcg aaaggcttta ttcttaaatc cacgtttacg aatctctttc
    31921 cacacttgtt caatgggttc atctctggtg tgtatggagg aataaaggta aaatcaatat
    31981 tagtcggaat ctttaaggta cttgatttat gccatatagc attgtccata acgagtaaaa
    32041 aataatcatc tggataagct tgtgaaagct cttctaaaaa gacgttcatc cactcagtat
    32101 tacatccacc agctattaag aaaaatgatt cgcctgtatg gggcatcaac agctccataa
    32161 caatagcgaa attctcgtat atagtgacta tggacatgtg gacctactcc tattggagac
    32221 caacaagatc ccagtttact gattctaccg aaaccagcct catcttggta catcaagcga
    32281 accttatgaa aaggtctact ggttttaaag cgctttcttg tcttcttgaa ttgagatttt
    32341 atttttagac gcgacaatgg tttgagcgtc tgctttccta ggatgttctg gacgtggcat
    32401 aatatttcgc caaccatggt gcttcaacag ttgatagaag gcatcacgtg tgtaggaacg
    32461 atataagcct gaaataaggt attgtaacaa attctcctgc ctctgtagcc ttcaaatggc
    32521 gggcaagaaa ggctttctct tcctcaactg tcatatatgc atggttacga ccaccacgtg
    32581 tttcttgaag gagagagtcg agtccgaact cctcatattt ttttacattt cgccaaatcg
    32641 ttgtttgatt acagtctaaa agatctataa tctctttata agatttgccc atcagacgaa
    32701 atagaacgat ttgaaggcgt ttatgatatt tagctgtacg aaagtctttt aaaagtgttt
    32761 tgatggtttg gatttcttct ttagttgatt tcatattact attgtataat gctttttgat
    32821 ttgagtctag tattatactc ttcgaaaatc tcttcaaacc gcgtcagctt cgccttgtcg
    32881 tatagaatgt tactgacttc gtcagttcta tctacaacct aaaaacagtg ttttgagcag
    32941 tctgcagcta actttctata acaaaatgaa ataagaacaa gacaaatcaa tcaagacaat
    33001 caaatcgatt tctaacaatg ttttagaagt agatgtgtac tattccagtt tcaatatatt
    33061 atagtttgct ctttgatttt cattgagtat tagttcaata tctaaaaaaa gag
//
LOCUS       DBEJAQ010000024        32779 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.024, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000024 DBEJAQ010000000
VERSION     DBEJAQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.024"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(139..1161)
                     /gene="cbpF"
                     /locus_tag="ACL297_001157"
     CDS             complement(139..1161)
                     /gene="cbpF"
                     /locus_tag="ACL297_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HGR0071427.1"
                     /translation="MKLLKKMMQVALATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRQEIAFRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWV
                     YDDGDWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQHLGNKWYYLRSSGAMATGWYQDGSTWYYLDAENGDMKTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(1180..2001)
                     /gene="cbpG"
                     /locus_tag="ACL297_001158"
     CDS             complement(1180..2001)
                     /gene="cbpG"
                     /locus_tag="ACL297_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HGR0071428.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHSYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2082..2321)
                     /locus_tag="ACL297_001159"
     CDS             complement(2082..2321)
                     /locus_tag="ACL297_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071429.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2471..3229)
                     /locus_tag="ACL297_001160"
                     /pseudo
     CDS             complement(2471..3229)
                     /locus_tag="ACL297_001160"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3230..3862)
                     /locus_tag="ACL297_001161"
     CDS             complement(3230..3862)
                     /locus_tag="ACL297_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGR0071430.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(3876..4871)
                     /locus_tag="ACL297_001162"
     CDS             complement(3876..4871)
                     /locus_tag="ACL297_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGR0071431.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4868..5566)
                     /gene="liaF"
                     /locus_tag="ACL297_001163"
     CDS             complement(4868..5566)
                     /gene="liaF"
                     /locus_tag="ACL297_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HGR0071432.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5643..6653)
                     /gene="fni"
                     /locus_tag="ACL297_001164"
     CDS             complement(5643..6653)
                     /gene="fni"
                     /locus_tag="ACL297_001164"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGR0071433.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6637..7644)
                     /locus_tag="ACL297_001165"
     CDS             complement(6637..7644)
                     /locus_tag="ACL297_001165"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HGR0071434.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDILIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSFEEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7631..8584)
                     /gene="mvaD"
                     /locus_tag="ACL297_001166"
     CDS             complement(7631..8584)
                     /gene="mvaD"
                     /locus_tag="ACL297_001166"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HGR0071435.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEYLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8566..9444)
                     /gene="mvk"
                     /locus_tag="ACL297_001167"
     CDS             complement(8566..9444)
                     /gene="mvk"
                     /locus_tag="ACL297_001167"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HGR0071436.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITETCVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9566..9790)
                     /locus_tag="ACL297_001168"
                     /pseudo
     CDS             complement(9566..9790)
                     /locus_tag="ACL297_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10506..11381
                     /locus_tag="ACL297_001169"
     CDS             10506..11381
                     /locus_tag="ACL297_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGR0071437.1"
                     /translation="MYPPELDSKILSIIKKAVTIRTTHTYEYQYSLLFGDAGYLWLLL
                     HLFSISKNQYYLQLANVTAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLK
                     IFIHNSIGEIYHYFLQRDTAKESILDYSFAHGYCGIAYALFAYSKVLEPSMFYNDLHT
                     FHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILYLCMYDCDGNKDIISKYQEFVFN
                     HHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILACSERDDHGLLMFQGDSGKAD
                     LFDFGIGSMGYIGVY"
     gene            11424..12656
                     /locus_tag="ACL297_001170"
     CDS             11424..12656
                     /locus_tag="ACL297_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGR0071438.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(12774..13772)
                     /gene="cbpJ"
                     /locus_tag="ACL297_001171"
     CDS             complement(12774..13772)
                     /gene="cbpJ"
                     /locus_tag="ACL297_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HGR0071439.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(13790..14812)
                     /gene="cbpC"
                     /locus_tag="ACL297_001172"
     CDS             complement(13790..14812)
                     /gene="cbpC"
                     /locus_tag="ACL297_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HGR0071440.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            complement(14910..15599)
                     /locus_tag="ACL297_001173"
     CDS             complement(14910..15599)
                     /locus_tag="ACL297_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGR0071441.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGIGYTMQE"
     gene            complement(15611..17035)
                     /gene="gndA"
                     /locus_tag="ACL297_001174"
     CDS             complement(15611..17035)
                     /gene="gndA"
                     /locus_tag="ACL297_001174"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGR0071442.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPYLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(17111..18505)
                     /gene="mapZ"
                     /locus_tag="ACL297_001175"
     CDS             complement(17111..18505)
                     /gene="mapZ"
                     /locus_tag="ACL297_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HGR0071443.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(18518..19675)
                     /locus_tag="ACL297_001176"
     CDS             complement(18518..19675)
                     /locus_tag="ACL297_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGR0071444.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREMRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLCSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(19760..20140)
                     /gene="rnpB"
                     /locus_tag="ACL297_001177"
     ncRNA           complement(19760..20140)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACL297_001177"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(20161..20490)
                     /gene="gpsB"
                     /locus_tag="ACL297_001178"
     CDS             complement(20161..20490)
                     /gene="gpsB"
                     /locus_tag="ACL297_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HGR0071445.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(20560..21087)
                     /locus_tag="ACL297_001179"
     CDS             complement(20560..21087)
                     /locus_tag="ACL297_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HGR0071446.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            21153..21749
                     /gene="recU"
                     /locus_tag="ACL297_001180"
     CDS             21153..21749
                     /gene="recU"
                     /locus_tag="ACL297_001180"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HGR0071447.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            21746..23905
                     /gene="pbp1a"
                     /locus_tag="ACL297_001181"
     CDS             21746..23905
                     /gene="pbp1a"
                     /locus_tag="ACL297_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HGR0071448.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(24072..29375)
                     /locus_tag="ACL297_001182"
     CDS             complement(24072..29375)
                     /locus_tag="ACL297_001182"
                     /EC_number="3.2.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpGH101 family
                     endo-alpha-N-acetylgalactosaminidase"
                     /protein_id="HGR0071449.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDERDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KQALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEITVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASVTVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(29676..31658)
                     /locus_tag="ACL297_001183"
     CDS             complement(29676..31658)
                     /locus_tag="ACL297_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0071450.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKVLDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            complement(31836..32636)
                     /locus_tag="ACL297_001184"
     CDS             complement(31836..32636)
                     /locus_tag="ACL297_001184"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGR0071451.1"
                     /translation="MIVNASDILGILYIFYSIIHFCIQKVAGASVRVPTMIFNLLNIY
                     KFPLGVSWYLYTLWSILIVYGLLSVVFKNRKSLLLVSVFAYIFTLFIQTDIFIVQRTL
                     VWGICFFLGSVLSEIHFDKINLKKFLFFFVLFDFIYMFAWFLFYEVGSKKDYVSYINP
                     GLWGIAFIVCVLVAFAIFPKMEKNFPKTFLYFTKYGKDSLGIYILHAPICSMIRILML
                     KVGINSVFLHVVVGIVLGWYLSILATYILKKIPFLNIVLLPQKYIKLK"
     gene            complement(32521..>32779)
                     /locus_tag="ACL297_001185"
     CDS             complement(32521..>32779)
                     /locus_tag="ACL297_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071452.1"
                     /translation="IPRKSSKLKPLIAEDKAYNHALSKDRSKVENILAKVKTFKMFST
                     TYRNHRKRFGYFRHTIYFLFNYSLLYSKGCGCLCPSTHYDI"
BASE COUNT         9503 a         7412 c         5777 g        10087 t
ORIGIN      
        1 ccttcggata aattaattgt ctaactgttt gagggcagta cagaaaaacc ttgatactat
       61 gcgttttttg attgtgaaga cttttatcca cctattatac aaagttaagt atccatcaca
      121 gtttacaatt agccttcatt acttaaccca ttcaccatta tagtttaagt agtaaccacc
      181 tactgtggta ttaacagcta aagcacctga atcataggca tagtaccagt taccattgac
      241 ttggaaccag cctgttttca tatctccatt acttgcattt agatagtacc aagtcgaacc
      301 ttcctgatac caaccagttg ccatagctcc tgatgaacgg agatagtacc atttgttccc
      361 aaggttttgc caaccagttt tcatatcgcc attttcagca tctaaatagt accaagttga
      421 accatcttga taccagccag ttgccatagc tcctgatgaa cggagatagt accatttatt
      481 accaagatgt tgccaacctg tttgcatgat gccagttgct ggatctagat agtaccatgg
      541 agctgctttt agcttctctt catcataggt aagagggtaa tccttaaccc aaccacgtgc
      601 tagctctcca accgtcaatc tgttgatgcg agaatcaaag ccaccatcct tctgcaaata
      661 ataccagtcc ccatcatcat aaacccaacc agtttttaaa gtatgataac tacgctgatc
      721 ttcaaaataa tagactcgtt tctctgctgg gctatcatat tcttccccat gatgtttgtt
      781 ggtatttgta gcagtttttg cttctaacac ttgcttgcca acaaattctt gtagtacccc
      841 atcttgacca aagtaaaacc aatctggtct aaaagcaatc tcttgtcttg gagaaggacc
      901 aatcgtaacc cccttgtgtg gagcaggtat atattgccag ccgacaacca tctctccgga
      961 taaaggatca aaataatagt atttcccatc tatcactctc cagtaggttt cttttagatc
     1021 cccctttttg tagtaggttc taccattttc ttggacaaac tgccatcctt cagaatcatc
     1081 tgcaaatact gtactggtcc ctagcaaacc gaagaaaaat gtggctagtg cgacttgcat
     1141 catttttttc aaaagcttca ttttatatat cctccaacat taaatccact caccagatga
     1201 agcgaagtta taaactttac catcgatagt ttggctacct gtaaccattg ctccatttga
     1261 attgagataa taccattttc catttacttt ttgccaatct gtaagcatct taccggaact
     1321 gtctaaataa taccaagtat catttatctc ctgccaaccc gtttgtttat catgttgtct
     1381 ataatggtac caactaccat ttattttctt ccatccttca agagagtaac ctttaagaac
     1441 cgaataaata aatggcaaat ttcgttcatt taatttaact gcactgttaa tttgattagc
     1501 tccatctcct aaagtatgca ctcctactac acggtgacta gcatcataaa ctgtagatcc
     1561 actagacccc tctaaagtat caacttggta atccaagaac atgccatcat cacttaaaac
     1621 ttgtttttta tctgtataca tttgatgaac tttaaaatta tatgatggat agcctgtgat
     1681 agtcacagtt attcctgtca aatttttttg actagtagga agacccaaag tccctaattt
     1741 tgcaccaatg ggctcttcta gaattaataa agccaagtca tattcccttg catccttaga
     1801 atttaaattt ctaaattcct tcaaataacg aacttccttt actttgatct ttccaaatgg
     1861 ttcttgactt ggactaacag ccggaagaac ataaatatca tccgcttctt tcccatagtc
     1921 atgtctgtag tagctgtgag ccgctgtaat taacacatta tctttaataa aattcgcact
     1981 tcccctccct tctacattca ttccatcggc tactccataa tacttagata aaaccatagc
     2041 tgaagtcgaa tacggatact gtaaagtatt atcaatttta atcaaatcat cattaccgat
     2101 aatacttctg attgcttttg gtagtatgaa ccatatgttg gtgaaatctc agataatgaa
     2161 gaatcattag atcctggacc tttttctagt gtctcactta cctcatattc ttcacccgta
     2221 ctagaaataa cactcaaagc agatactgtc gataactggc tagccaataa agtactcgca
     2281 ataattgaaa tacccaattt tttataaaca gttttcttca ttattgtatc ctcctaatgt
     2341 aattatagcg tactattcta aatttcttaa tctactatag aatcaagaaa tctaccacct
     2401 tctttaaata ccctccatta tcacataaac aggtaaactt ttcaattaat gactgcgctt
     2461 ttcaatcacg ctagaggtac ttacttgctt ctttgatact aagttcagcc attctttcct
     2521 tgtttttctc aataaagcat gttacccaag tgggattcgt tttggagtag tctcgcagag
     2581 tccagccaat ggctttattg ataaaaaatt ctgtttggtt caagttatga aggagaatct
     2641 tttccattaa ttgagtattg gtcttctctt ttcttaacaa ctggttgtca atagcgacac
     2701 gtctcagcca gatattatct gataggctcc attttatact caatgaaaat caaagagcaa
     2761 actaggaagc tagccgcaga ttgctcaaaa cactgttttg aggttgcaga tagagctgac
     2821 gtggtttgaa gagattttcg aagaatatta agattatttc ttctagttca ggatgtttat
     2881 acaccaaact ccctactact cgatctagga tatctaccgt gtcccaccag gattttgtca
     2941 cgactaactg ctctagctta ggcaaatcgg tttcctttag ataagactgc attgctttca
     3001 aatagttagc agccacatat tggtattttc taggctcctt ttcccagcaa gtgtctgcaa
     3061 aatcccaatc gataatcttt gtttttttcg cttctggaaa atatttttat agagtttatt
     3121 tctttcaacc tagaaaagaa aattgatggc acatataggc ttccatgggc cttgcttttt
     3181 tagagtcttt tgctgcttct agctcctcaa gtaaatctgc taaactcatc taaaactcct
     3241 catgccccac caaatggtgc tgaaaggcat agacagccgc ctgggtacga tcgctaactt
     3301 caagtttggc aagaatgttg gacacgtggg tcttgaccgt cttgagagag ataaagaggt
     3361 catctgcgat gcgctgattt tcgtagccct tggcgatgag ttggagaaca tctcgctcac
     3421 gcgcagtcaa ttcttcatga agttccatat gattgcggtg gtattcaacc ttcttgctaa
     3481 cctcttgctc aatggccagc tcgccagcag ctaccttact gacggcatga agcaattcat
     3541 ctgcactaga agtcttgagc atatagcctt tggcaccagc atctaagact ggcatgattt
     3601 tttcattgtc caaataagag gtcacaatca aaatcttggc ttcaggccat tctttaagga
     3661 ttgctaaggt cgcgtcaatc ccattcatct caggcatgac aatatccatg acaatgacat
     3721 ctggacgcag ttccaaggcc aagtcaatcc cttgagaccc gttggacgcc tcacccacaa
     3781 cttctacatc gtcttggagg tcaaagtagc ttttcaagcc caatcggacc atttcatggt
     3841 catctactag taaaattttc atctttactc ctttatcatt ccttatctaa caggggaata
     3901 cggatatcaa ccgccagccc ttgcttggga gctgtcaaga gttgaactgt tccagccata
     3961 tcttcaaccc gctccttgat atttcgcagt ccataactca agtcgtctaa gctccctaac
     4021 tggaaaccaa tcccattgtc caccaccttc agttgcaatt caacatctgt ctgatagagg
     4081 tagacatcta ggcaagatgc ctgggcatgg cggagggtat tgctaatcaa ctcttgcagg
     4141 atacggaaga tatgctcctc gattttctta ggcaatttcg tcatattctg cttgagacta
     4201 accctaagat cactcttgtc ctcaagctct tttaagagaa tctgaatccc ttctatcaag
     4261 ctcttctgct ccagttcaac tggtcgcaaa tgcaagagca aaacccgcaa atccttctgg
     4321 gctgtttcta aaatagctgt gacactctgc aactggatct gcatcttttc tctatccaat
     4381 ttcaaagcct gctgactgat acccgataaa atcatgtggg ccgcaaacaa ctcctgactg
     4441 actgtatcgt gcaaatcccg agcaattcgc ttccgttcct tctcgatgat ttcctcttcc
     4501 tgagcaaggc tctgattttc agctttttga agagcttctg tcaaaaggtt aagtttacct
     4561 gataaggact tgaaactggc atccaaatct ggatctgcaa cctgaaccac ttcttgccct
     4621 gccaataaac gcttgagatt agcctgcatt tttcttagag aaagctcttc gatccctcgc
     4681 caaaacaggg ctaagagaca ggttatggac atgctgaaaa ccaacaataa aaagacaaat
     4741 ttttctgttt tttcgacatc gtgcaaaaag atagaccagt caaaatcaag tatttccagc
     4801 aagctgtggg agaaaaaaaa gacaaacagg aaggaggtga gagcaataat gacataggct
     4861 tgttttttca tcctctaacc acctccacat caccaatcat agtggtcaag aaaatcttga
     4921 cactcttgtt actcttgaga tagtcttttg tttcttgatg atagtgttca ttgcggaggg
     4981 ctcgcttggg ctggttgaaa aaagtcaaat ccccatagag acagttaacg ctgagactga
     5041 cttccacatc tacaggtacg atgattttgg tcgttcctac catctttctg aggataatga
     5101 cattgtcatg attggttaag atgaccctct ccagatgaat agtgtccttg cccatgaagc
     5161 gaaagagatt gatatcatcg aattggcaag tctggtagct tgaaaaatga tgaagatttc
     5221 caaaccaacg atttttctcc ttcttaaccg tcacgacctc ttcaaaaacc aaattggtct
     5281 gctctttttc ctggttcatc atcgggtaaa gaagaaagag gctatagata accgcaacaa
     5341 aaatagctag aatcacaaaa ggattgagca taacgatgaa aaagaagaga atggttgccg
     5401 ctactaaaag aagattattt ccctctttac cagtgtagta gcgaatcaaa agcaaaaaga
     5461 ggaatagtat cagcagaaaa cgcgaaaaat gctctgatac catcaaaatc agagctcctg
     5521 tcagaagaca ggcttcgata aataaaaaga ttttaaattt tctcataggt tcatcctctc
     5581 ccttctattt tatcacaatt caaaaaagtc acctcagtct gaggatggaa aaaaggcgct
     5641 ggttacgcct ttttcatctg atcctttgct tcttttaatt ttccataaag aagatagtct
     5701 actttttgta gatctgctat ggtggcacag ttaagggaac acataatcaa gcgtaggtct
     5761 gctttccagc cttggacaat gccaatcact tcttcaactg tgtaggtttc aaccaattcc
     5821 agaacggttc gtgacaatcc cacagactta gcaccaaaaa ccaagcactt aatcatatcc
     5881 agcggattcc gaacccctcc actaaccaag agttcgacct tatctttcca ttcttgggca
     5941 ttgagaaggg cctgcatggt agactgaccc cattgattga ggtaatcacg ctggccacta
     6001 cgacggtttt cgatataggc aaagctggtg ccaccacgac ccgataggtc cactgtacga
     6061 acaccgaatt cataggctct ttcgattgtc ttggcatcca ttccaaagcc cacttccttg
     6121 aggacaatag gaacgggaat ttgctcgcta taatctgcta gatgcgattg ccagcttcta
     6181 aacttccttt ctccctcggg catgagtaat tcctgcatga cattgacatg cacttgcaat
     6241 agaacaggat tcatctcttc tacagtctga agtcctaact cgacaggctt gtccaatcca
     6301 atattggttc caaggaggag attgggatga ctagacttga cagaaaaaga atcatccgtt
     6361 ggatttttga gggctgcgct ataagaaccc gttacaaata aaataccaca ggattccgcc
     6421 acctgagcca gcttttgatt gatttctctt cccttattac ttccaccagt catggcattg
     6481 atataaaaag gaaagtccca ctttcgacca gcaaactctg tcgaaagatc gatttcatcc
     6541 agattgtaaa gaggcaagga agaatgaatc agctccacct catcaaagct attataggaa
     6601 cttttctgct caagggcata gaggatatgc tcgtccttac gatttgtcgt catgtcctat
     6661 cctttcttga tataagagct caatccccag atcggcccaa cgatttttta aggttttggt
     6721 tgattgcgca tcaaaactca gggcgatgcc acagtcacca ccaccagcac cactactctt
     6781 ggcaacggcc tgcaaatctt gactggcttc tttcaactgt ctaagcaaag gcgtgtaaat
     6841 atctgtactc aagccttcta aaagcttgct ggctacttct acttgctcga taatcttttc
     6901 tgatttcccc tgttccaagg cttcctcaaa agaaaccacc gtttcttttg aggaagttaa
     6961 aaaattttga ttgatatttt gcttgatttg ctggaccatg tgactcgata cagccacttc
     7021 cttggtccat cccactaaga aatcacattc taaagttggt ttcacttgtg aaattgaaaa
     7081 gccccaatca cgctccagaa ctgtcgccaa gttttcttct tctaaccaag cagccacctt
     7141 ctggcgatca aatgactggt agagaaccaa atcctctgcc acaatacagg caaggtcgcc
     7201 catggaacca ttgtctcctc gcttgagcaa gacagcgcta gtcagcttga acaagagctc
     7261 ctgatcaaca gaaacatcat acagagccag taaagccttg acaaccaaga caacgacgct
     7321 gccactagaa cctagaccaa actttttccc ttctcgttcc attttgccac agatttctag
     7381 agaaaaaggt cttaaattct gaccacgaac agcgaggaag tctcccatca aagcaatcgt
     7441 ttcttgaatc aagctgtagt caggattagg ccttaagtcc actgcgaaat caaacatatc
     7501 tgaatagata cggtagctgt cagaaaaagc aatctcagcc ctcatataga tgagaatatc
     7561 ctttatcaaa gctaactgcc ctggctctaa aatagcatat tcacctgccc aatagagttt
     7621 tccgcaagtt ttaacagcaa tcatcttgac tcaaatcctt tgtttttgac acaatcaagc
     7681 gataacgctg accgaaaatt tctgacaaat actccaagtc tttctcctga cagaagacct
     7741 taacattggg accagcatcc atggtaaagt agcaggcctc tcctttctca cgaagctggc
     7801 gaacaaagtc catagcctca taagaggcat ccgtcagata agaaaaggct ggactagcag
     7861 tctttgtcgt agcatgcata gccagggcat ttttctccgt taattctcca atcttggcaa
     7921 aatcattttc cttgagataa atcagcatat cctgatagtc cttctcagac tgacgaaccc
     7981 agtcgtcgaa agtcgtcgag gtttccacac aaagtttcat cccgtcacgg ctagagattg
     8041 gttttttctt gtcctctagc accaacataa tcatagctag tttcaagtct gtctctacag
     8101 ggtaaatttc tccactatcc ttatcccagg ctcctagtgg tccataaaaa ctccgagaag
     8161 aagagcctga ggcaaatttg gcttcctgtg ccaactgact tctatccaat ccaagcttga
     8221 aataagcatt acaagccttg accagggcgg acaaaccact agaacttgag gacaggcccg
     8281 ctgccgtagg catattgttt tgagtatcga tacggacaaa gccctcacca gctggacgat
     8341 aacggtcaat aatcttactc atcttggcat gctcgacctc attttgtagc tgaccattga
     8401 tgtaaaattc gtcagctgtt acattggctg gtaaaggcga caaggtcgtc tctgtataca
     8461 tattttccaa agttagagaa atactgctag tagcaggcac catctctttt tcttttttct
     8521 ttccccaata tttgataata gcaatatttg cgtaggaacg tactgttaca ggctctctat
     8581 ccatgtctga acagctcctt tctcttctaa tctttctgct agttcttgtg cgtgtgtcaa
     8641 attggttacc aaggctatga tacaacctcc tagcccacca ccgctcatct tggcacccag
     8701 agcaccatgg ctaagagtcg tttcaaccaa aaagtctgcc tcagggctac tgactccaat
     8761 ttcttttaaa tgtaaatgcg cttgactgag gatttgtccc agtccttcag catctttttg
     8821 tgaaatcgca acttctgctt gctgggttaa ttctcccaag gcatgcaaaa acggtagggc
     8881 atccttgccc ttattttgaa ccacttggat ggcttcacga gtatgaccat aaacacccgt
     8941 atcggcaatc accaaatagg cggataaatc catctcaagt tctgtaaatc ctacgttctt
     9001 gataaagcga ataggttggt cactgagaca ggtcttagca tccaaaccac taggattcat
     9061 atgagcaatc atctcagctc gattgaccaa gatttctagt acatcatgag gcagatcagc
     9121 ctgatagtag tcaaataccg cacgaatggc cgctatgctg atagccgctg acgaacccat
     9181 cccccgtttc tcagggatag ccgagtcaat ctcacaacga acgcaggttt ctgtgatatc
     9241 caaatactcc agtgaagcat aaaccgccat ggacaaggta tcctcctcat aaaggcgcca
     9301 aggactctct gcaggaacta ccttacaggt cacctccacc tccaaaagag gcagggaaat
     9361 ggcaggataa ccgtaaacga ccgcatgttc ccctattaaa attatcttac tatgtgcctg
     9421 accgacacca actttttttg tcattttttc cttttactag acgaaaaaac gtcttatttt
     9481 tcatacaagt attaattctt tcctatctat tttattatat tttcacaaaa aaagcgattg
     9541 tttccattca caatcgcttc tttcattatt gaacccattc gccattataa ttgacaaaat
     9601 agccatctac ggtcgtattc actgccaagg cacctgagct ataagcgtag taccatctgc
     9661 cattgacctg gaaccaacct gtcgtcatag aacgacgaaa gaaactccat accattaagt
     9721 aaagaggaaa gtcgtgaggg agcatgcgcc attgacaacc tgttttagtg acgtacaaag
     9781 tctcattaac aagtactcgt ttcggtcatt tataggtgcg gtgtttggag aaatagggtt
     9841 caatcttagc ccattcttga tcgtttaaat cagtatcata tactttgcgt gtcataactc
     9901 tagtttaaca ttttttgtga atacaggttc taaataatcg accacgaaaa tttcttaagt
     9961 ggaaaacgcc ttatgaagta tgctacggga aagttatgca cttaatttga caattcaaga
    10021 tgtaaaaata tatactatag tagattgaaa ctagaatagt acacctctac ttctaaaata
    10081 ttgttagaaa tcgatttgac tgtcctgatc gatttatcct gttattatct cattttacta
    10141 taatatttga taagttatcc taaaagtatt attatgttgt tgtgttatag attgattgaa
    10201 tctaactaaa ggatcctatt caattactag aactatcaca tactcaaggt cagctcacag
    10261 atgagcaact attttggtta caatgtctac taaatttaag tcaaacaaat aatttagtca
    10321 aaattaaaaa aatagaggaa cataaatatg attacaaaac agaatgtaat agtgttctac
    10381 aatttttact agataaaact gtaaattctg aaggaaggat cacttcttca acagaatttg
    10441 gaaatttcgt aagtaattta tcattccaac acggaatagc tggactactg tttcctctaa
    10501 ataaattgta ccccccagaa ctggattcta aaatactctc tatcatcaag aaggcagtga
    10561 caattagaac gacacacaca tatgaatatc aatactcact gctatttggt gatgcaggct
    10621 atctatggtt actcctacat ttattttcta tcagtaaaaa tcaatactat ctacaattag
    10681 caaacgtcac cgctaaaaaa ttaatagaga attatgatac tctagaggaa atagactttg
    10741 cattgggaaa atctggtgtc ctattatcat taataaaata ctatcaattt accaatgaca
    10801 atactcttaa aattttcatc cacaatagta taggggaaat ttatcattat ttcctacaaa
    10861 gagatacagc caaagaaagc attttagact atagctttgc tcatggatat tgtggaattg
    10921 catatgcttt atttgcctat tctaaagtct tagaaccttc tatgttctat aatgatctcc
    10981 atacattcca tactgaatta aaaaaattat tagaaaaagt tacttctaat actgaaaatt
    11041 taggaaattt acaactttct tggtgcaaag gaatttccgg aataatctta tatctttgta
    11101 tgtacgattg tgacggaaac aaagatatta ttagtaaata tcaagaattt gtttttaacc
    11161 atcatctaaa aatgatgaca ggatattgcc acggaataac tagcttacta caaaccactg
    11221 tctacaatca aaacaaatta ctgatgaaaa aaatccaaca ggtaatttta gcatgttctg
    11281 aacgagatga tcacggttta cttatgtttc aaggagatag tggtaaagca gatttgtttg
    11341 acttcggaat aggaagcatg gggtatattg gtgtctatta aataataaat tcccattcga
    11401 tgtgcagaca taaggagaaa agtatgaaat tattttggac aaacaacata tatagacagt
    11461 tgctgctaaa cagctgtttt tcatcattcg gcgacagtat tttctacctc gccattatca
    11521 attatgtggc tcagtacaat ttcgctccgc tagcgatttt actgatttcc atttcagaga
    11581 tggttcccct actatcgcaa ctctttctcg ggattctagg agattttcaa gaaaatagag
    11641 tcaaacacgc actctggatt gccaaaatca aaatcctgct ctacgctatt ttgacagtat
    11701 ttctcgtctt gtcgcccttt tcattagttt cagtcattat gattgtcatc atcaacctca
    11761 tctctgacac cttgagctac ctgtctgcct acatgatgaa cgccctctac atcagtgtaa
    11821 ttaaggacga cctgcatgat gccatggggt tcaggcagtc tctgatgagg gttgtccgta
    11881 ttgtcgccaa tctggctggc gcattcctta tcaatgttat aagtattcaa actatttccc
    11941 ttatcaacac tctgactttt gtcattgcct ttttgggcct gtatgttatt cgacatacct
    12001 tgtatgaggt tgaaaaaaga attgaaatgt cacatacagc actgagtttt aagaaatatt
    12061 ttcaacatct taaacagtcg ctggctgtgc tcctgaggtt aaaagatacc gtcatactac
    12121 tgtttctgac gaccagtatg attgccatct tggatgtgtc ccctcggctg attgccctcc
    12181 gcttcatcca acagacacta gcacaactga gcattgggca actcctcgcc ctgctctcca
    12241 tcatcatgtc ttgtggagct atccttggca atatgaccag cagtaatcta tttaaaaata
    12301 tccgtttcac gcacctcttg gttttctgtg agatttccct attgactcta ataactagta
    12361 tcctttgtca agcctatatc gtaattttca tgaccagttt catcagttct acgattatcg
    12421 gcattctcag ccctcgccta caagcagctg tctttgccca tatccccagt gacaagatgg
    12481 ggacggtggg ctctgctctg agcacagtgg acattctcgc cccgtccctg ctctccctat
    12541 tagccctatc catagcatcg ggcgtttcgg tgcagttagc attgatattt ttgtatctta
    12601 ttttaattgc tcttatcttt tgtcaatggt tagtcaagtt caacactcat aactaacgaa
    12661 aaagcatgtg tagatttcac atgcttttaa tctccccaat cgtcaggtca agtacaacaa
    12721 agtcacttct ttgattaagc gagtgttcta atataattat aagcgccctg tcattaccga
    12781 acccattcgc cattatagtt gacagaatag ccatctacgg tcgtattcac tgccaaagca
    12841 cctgagctat aagcatagta ccagttgcca ttgacctgga accaacctgt cttcatgtct
    12901 ccattacctg catttaggta gtaccaagtt gaaccatctt gataccaacc agttgccata
    12961 gctcctgatg aacggagata gtaccatttg ttcccaaggt tttgccaacc tgttttcata
    13021 tcgccatttg ggtggtctaa ataataccaa gtggtacctt cctgatacca gccagtggcc
    13081 attgctcctg aggaacggag gtagtaccac ttattaccta gatattgcca acctgtttgc
    13141 ataataccag ttgttggatc taggtagtac caagtcgaat catcgtttat ccaccccgca
    13201 cgtctttcac caccaaggta gttttctcca ttaatttccg tcttagctag ataataccag
    13261 ttagactgat cataaagcca acctgtctct aaagaatgat tttgattaaa gtaatagttc
    13321 gtataataac gcttctcttc tttatcttct gaatcttcac gtttttcccc gtactttctt
    13381 ccaacactgt ctttagtttt aatctctaat gttttccaac caacaaactc ttgtagcact
    13441 ccatttttat cgaagtagta ccactctgac tttggaaaac cttctaatct gataccattt
    13501 gggtaaggac caattgtact acctttagat ggaaacggga tatattgcca gccgacaacc
    13561 atctctccag atagagaatc aaaataatag tacttaccat caatcactcg ccagtaggtt
    13621 tctttgaggt cccccttttt gtagtaggtt cttccgtttt cttggacaaa ctgccatcct
    13681 tcagaatcat ctgcaaatac tgtactggtc cctagcaaac caaagaaaaa tactgtcagt
    13741 ccaacttgca tagttttttt caaaattttc atctatatac cctccaatat taaatccact
    13801 caccagatga ggcgaaatta taaactttac catcgatagt ttggctacct gtaaccattg
    13861 ctccatttga attgagataa taccattttc catttacttt tgtccaacct attttcatat
    13921 ctccattaga aggatctaaa tagtaccaag ttgaaccatc ttgataccag ccagttacca
    13981 tagctcctga tgaacggagg tagtaccact tgttaccaag gtattgccat cctgttttca
    14041 tatcaccatt tttattatct aagtaatacc atttgcctcg tacatatttc caacctgttg
    14101 ccatagaacc agaggagcca aaataatacc attttccgtt tactttttgc caatctgtaa
    14161 gcatcttacc tgaagcatct aggtagtacc atggagcagg tttgcttcta tcaatgtcaa
    14221 tagtaacatg aaaatcttga gtccaaccct tagcaacttc accaattggt agtggattgt
    14281 aagaatcatc gccaaaattt cctagcttat ttaaataata ccaatgaccc tcttcataaa
    14341 tccagcctgt ctttacagca taattattat caaaataata agttctcttt tcagcagata
    14401 gaggatattg ttcaccgtat acttttcctg tattttcaga cgtttttgcc tctagtactt
    14461 gtttatctgt ttgttctagc aaagcaccat cttgtccaaa atagtaccac ttctcctgaa
    14521 ggccaatttc attaactggt tggttatcga ataaattatc acgataacct gttccaggaa
    14581 tttctaagta ttgccaaccg acaaccatct ctccaatatc accaaaatag taagttttac
    14641 cgtctatttt atgccaatag attgctttat gatcatcttt tacataatat tttctattat
    14701 ccttatcaac aaatcggcca cctgtggtat tcgcaaatac cgtatttgta gctagcaaac
    14761 caaagaaaaa gactgctagt agaacttgca tcattttttt caaaagtttc atctatatat
    14821 cctcctaaaa cgattgtagc aaagacccga ctgcatgtca atatataaaa actcctcaaa
    14881 aaagcagtta cacaactgca actgcttttc tattcttgca tggtatatcc aataccacgc
    14941 acagttttaa tgtagctttt ttgtccttta acatcaagct tgctccgtag atagcggata
    15001 tagacatcta cgatatttgt ctcggtcgca ctttcatact tccaaacact ttccaacaat
    15061 tgctcacgag tcaatacttt cttgcttccc ataagtgtcg ccaaaaggtc atactcacgg
    15121 cgggtcagag caatcatctc ttcaccacga taaaccgtgt gatgttcaac atctatccta
    15181 agattgcggt aggtccttgg aactttcatc agactgcagt gttgatcaat gaagtcccga
    15241 cctcggaaga tcgccgaaat acgcgctacc agattttcga taaggactgg cttatagatg
    15301 tatgaaactg caaaacgctg aacaacttcc agctcttctt gcaagtcttc ccaatgatct
    15361 aaaatcatga tgactgaggc aggtttagtt cggctcaatt tttctgcaaa atcctgagcc
    15421 atcatatctc ccagattaac gttcaataac atcaaatcat agtctgtctg aagagccatg
    15481 gagagggctt tttgcccctc ctctaccaga tcaacccgat actgctcttt ctggagttcc
    15541 aaacttaaaa aatgagctag atttcgttct ttctcaagta ataaaatccg tttccccatg
    15601 gctgacctac ttatttttcg tcataccaag agtagtggaa ggttccttct ttgtctttac
    15661 gttggtaagt gtgagcacca aagtagtcac gttgtgcttg gatcaagtta gctggaaggt
    15721 cagctgaacg gtagctatca aagtaagtaa tagctgctga gaaagttggc actggcacac
    15781 cagcttgaac cgcaagagct acgatatcac gcactgcttg ttgatactta gcagtaacat
    15841 ccaagaagta ttcatccaaa agaaggttgg caaggtctgc atcacggttg taagcatctg
    15901 taatcttttg caagaaacga gaacggatga tacagccatc acgccagata gatgcgatat
    15961 ctgcaaatgg caagttccag ttgttttctt tagaggctac acgtaattga gcaaatcctt
    16021 gtgcgtatga aatgattttt gaaaagtaaa gggcttgacg gatcttttca atcaattcag
    16081 ccttgtctcc ttcaaagttg aaggcagctg gttttggaag caccttgcta gcatgtacac
    16141 gttcttcttt gtaagttgaa atgtagcgtg caaacactga ctcagtaatc agtgacaatg
    16201 gtacaccaag gtcaagagat gattggctag tccatttacc agttcccttg ttacctgcag
    16261 catcaaggat gtagtctacg attggtccat cttggccttc atcgtcttta cggctcaaaa
    16321 tatcagctgt gatttcgatc aaatagctgt ctaattcacc cttgttccac tcagtaaaga
    16381 tttcagccat atcctctgca gaaaggccta gcaagtgttg catcaagtca tagctttctg
    16441 cgatcaattg catatcaccg tactcaatac cattgtgaac cattttcaca tagtgaccag
    16501 ctccatcagg gccgatgtaa gtcacacatg gtttgccatc ttctggtgct ttagctgaga
    16561 tttcttcaag aacatccgca accaattcgt aggcctcttt ttgtccacca ggcatgatag
    16621 aaggaccttc aagggcacct ttttcaccac cagaaacccc agtaccgata aagttgatac
    16681 ctgagtttgc caattcttca ttacgacgga tggtatcttt gtagaaagta tttccaccgt
    16741 caatcaagat atcacccttg tcaaggtatg gaagaagggc ttggatagta gcatctgtac
    16801 caggtccagc ttgaaccatc agcatgatac gacgaggttt ttcgattgag tttacaaaac
    16861 tttcaacgtc atagcttggt acaaagttct tttcaggatg gcaagcaatc acatcttccg
    16921 ttttttcttt actacggttg tagatagcaa ctgtgtaacc acgtgattca atattaaggg
    16981 caaggttacg acccattacg gccataccta caacaccaaa gttagctttt gtcatttgac
    17041 actcctcttg tttcatttgt tttattttat catttttact tttgaaaaga aagaattttg
    17101 taacgactgc ttagtaatcc aagtcatccg catgaccagc gccatttccg acaaagtagc
    17161 ctgtcttaca gttaagggta aagagatagg ttccatctgg cttgtagagg ttgtaataac
    17221 cattgccgtt aacgatattg acacgttcaa ggatatattg gtctccagtg atatagccac
    17281 gtgaacgtga agttgctaga atttgttcca agacaccatc cgcaaaatcc caggcagagt
    17341 tattactatc atcaatagca gactgattat aggcaacacg actggcactt ctttgaacag
    17401 caacccctgc actcgagaga cccatattga cttcactgcg actacttcta gtctcatttg
    17461 aactacttgg ttttggctca ctagtcgtat ttgagcttgc ttgactagag cttgagctgc
    17521 tagtttgact tgaacttgag ctagaagtac ttgtttgctg gctcttacca agactgatag
    17581 ccttatctag cactttatca agctccgtat tcccagtttt aatatctgta aatttagcat
    17641 ccgatttggc tttggcattg gtatccaaca caccatccac aatagctggt ttttcaaatt
    17701 gtgcattgac atcttgaatg gccttaattt gcgttactag actatcatat ttagatttgg
    17761 caagcgtatg ttcacgacta ccttccagct tatcaagtaa agtcttgagt tgactcagtt
    17821 tatcaaactg gctatttttc aaagccgttt tattgctatc tgtgtaaaag gcgtcataaa
    17881 gtgtattaaa atcatccaca tccgattgat tggctgtagt tgattgagaa gtttcaattt
    17941 ccttagtcga acgagccact tgacggtaga cataataagc actgacacaa ataattactg
    18001 atacaagcgc caaaacggtc aaaataaagc cttttttact tttcttttct gagtcctctt
    18061 gatccagacg agaaaggttt tgctcctctt ccacttcttc agtcatttct gttggatttt
    18121 catcatctag caatagagga tccaagcctt gctcctcatc gtccagaggc aagggtagta
    18181 agatgtcttc agaagatgga acttcctccg aagaagcttc tgacttctct acagcctcac
    18241 gcatctcttg aattaaatca tccagactct gagtttcaac gaactcctct tttttgtatt
    18301 gacgagtcgc aaacttatcc gcctcaattt catctctgtg ttgcttaaca tacttgtcca
    18361 aaatggaatc ctcaggcaag actcctgctt ccacttcttc atttttacga atagcttgac
    18421 caactgttag ctcttttgct tcatcaaaat caaattgcgg ttcttgacct tcttttttat
    18481 gacgatttcg tcttttttta ctcatgagta tccctttcta ttttacctct tgccgtttga
    18541 cacgctgacc aaaatattga tatagatcca ctttcaaggt tccgttgtat aacttacgct
    18601 tcttatctgc ttggctacca tacttgcttt caaaagcttc atcactagtc aggataaatt
    18661 tgctccaagt tttcagcggt gcaaatactt gccccatctc agcatagagc ttggtcaccc
    18721 ctgcatcatc tgacaaacgt tcaccataag gcggattgga aatgattact ccattgattt
    18781 tatcggaaca taaatcctgc acgcgcatct gcttaaaagt aatgtctcct gcaacaccag
    18841 ctacctgagc attggcctta gcaatttcca ccatgcgagc atcaatatca cagcccatga
    18901 tatccagctc aagctcacgg tctacttttt tagccgcttc tgtgcgcact tcttgaatca
    18961 agcgatcgct gatccagttc cattcctcaa atgcaaaaga acgacgaaga cctggcgcca
    19021 tctttctagc aatcataact gcctcaatac agaaagtccc cgaaccacag gtcggatcaa
    19081 tcaaaggctt gtctggatac cagttagaaa gttgtaaaat ggctgctgcc atattttcct
    19141 tgataggagc gccacctttt tcggtacgat aaccacgttt aaagaggcta gacccggtcg
    19201 tatcaatcat gacagttgcc acatctttga gaatagagac ctcaatctta aactctggac
    19261 cattctccat cagaggaacc ccttctgggc gagcatagtg tttctgcaat ttcttgacaa
    19321 cagctttctt agaaatagcc tgaacactgg gctcattgtg aagtttggac ttaacacatt
    19381 tagcttttga aatcgggaac cgagctccaa gtggtaaata attttcccaa tctaaagcga
    19441 aaactccctg aaatagctct tcaaaagtct tagctgggaa cgttcctacg ataattttga
    19501 tacgatctgc tgcccgaagc caaaggttgg tttcgataat agctctcacg tctccttgaa
    19561 aacgaacacg tccattttca acctgacaat cgtagcccaa ctctcgcatt tcacgaccaa
    19621 cgacagcctc aagccctgct gccacagttg caattaaatt aaattctttt ttcatgttac
    19681 agtcttgcaa gacctttcta tatgtccact ttaaaaaatg aggttgagaa aatttctcag
    19741 ccccaaccta tatgcaattt tctataagcc atgttttgtt ccagaagtga ctaggaccac
    19801 ttccttcgat aatcatctgt ctaccactaa cagctctagc tgatagcagt cagaatacta
    19861 cgttcgtttc cgcgtactcc atgccccgac caaaatttgg gttgctagct tgaggggttt
    19921 accgcgttcc actctctctg tttccagaaa gactccgtca ctgtggcact ttcaagccta
    19981 ctctggccta tccaaggact tagccatttc aactgccgta acgatttctc gtccctaggc
    20041 ttatggtttc gcctagcaca aacactacag gcatcacagc ctgtgctagc atggactttc
    20101 ctcatgagaa gcaattctcc tcacgcgatt atccaaaaat tgcacatctc aaataactac
    20161 ttaaaaatct gagttatcta aaatttgttt accaaaaact tctttttcca atctattcag
    20221 gcgtttcaaa atatcaaaat tcgtcataga acttgtaatt gccgcttcaa ggggttctgc
    20281 ttgaactggt gaaggtttcg gtttacgagt taattcttcc ttcaaatccg caatttcctg
    20341 acgaagtgac ttgaccaagg cagcataggt ttcatagtcc ttgatgacat cgtctaaaaa
    20401 ctcgtcaact tctactttat tatagccacg gacttcacgt ccaaactctt gttcaaaaat
    20461 atctttcgct gaaaaaataa tacttgccat gtctctctcc attctcgctt gctagtatta
    20521 ttatataaaa aaaggcaaac aaaaatcaag gtcaaagctt caattttcgg aaaaattttc
    20581 agcaagttca tttaattcat caaatgttaa tctctttata aaatagtcct cttgattttt
    20641 catcttttgg taaaaataag ctagttttgt ttcattttct tcatcataaa aaagatagga
    20701 actagtcgtg ttttccagca aaaactgctg gtaatctctt aactgcccct tgtgttcata
    20761 gcgaggatag gcatatttga caaagtctac ctgcttaaag cgactcagtt tcatctgatt
    20821 gccttcattc caattttccc catgggtttc aaaagcaaaa atggtggcca actggaaacc
    20881 gtattcagtt ttcatctcct gagcaacctc taaaacccaa tattcaaaac ccaaactccc
    20941 tgtaaacaca agccaagaca ccccatctgc tgccatagcc tctaaatctt tacgtatcgc
    21001 ttttttaatc aatttaagac gaggatcctt atcagaaaat aaacccaaat caaaagcaga
    21061 atagcctaaa accaaagctg tagccatttt taaccctttc tgtgcaaatt atggtataat
    21121 aaagtgatgt tattgtacca ataaggagaa ttatggtcaa ctatccacat aaagtttcat
    21181 cacaaaaaag acaaacatct ctttctcaac ccaaaaattt cgcaaatcga ggaatgtctt
    21241 ttgaaaagat gatcaatgct accaacgact actatttgtc tcagggcttg gctgttatac
    21301 ataagaaacc aactcctatt caaatcgtac aagtggacta tccacaacga agtcgtgcca
    21361 agattgttga agcctatttt cgacaagctt caacgacgga ctattctggc gtttataatg
    21421 gatattacat cgactttgaa gtcaaggaaa caaaacaaaa acgtgcgatt ccgatgaaaa
    21481 attttcatcc acatcagatt cagcatatgg aacaagtcct tgcccaacaa ggaatctgct
    21541 ttgtccttct tcacttttct tctcagcaag aaacctactt attgccggca ttcgatttga
    21601 ttcgcttcta tcatcaagat aagggacaaa aatcaatgcc acttgaatat attcgagaat
    21661 atggatatga aatcaaggct ggtgccttcc ctcaaattcc ttatctcaat gttatcaaag
    21721 aacatttatt aggtggtaaa acaagatgaa caaaccaacg attctgcgcc taatcaagta
    21781 tctgagcatt agcttcttaa gcttggttat cgcagccatt gtcttaggcg gaggagtttt
    21841 tttctactac gttagcaagg ctcctagcct atccgagagt aaactagttg caacaacttc
    21901 tagtaaaatc tacgacaata aaaatcaact cattgctgac ttgggttctg aacgccgcgt
    21961 caatgcccaa gctaatgata ttcccacaga tttggttaag gcaatcgttt ctatcgaaga
    22021 ccatcgcttc ttcgaccaca gggggattga taccatccgt atcctaggag ctttcttgcg
    22081 caatctgcaa agcaattccc tccaaggtgg atcaactctc acccaacagt tgattaagtt
    22141 gacttacttt tcaacttcga cttccgacca gactatttct cgtaaggctc aggaagcttg
    22201 gttagcgatt cagttagaac aaaaagcaac caagcaagaa atcttgacct actatataaa
    22261 taaggtctac atgtctaatg ggaactatgg aatgcagaca gcagctcaaa actactatgg
    22321 taaagacctc aataatttaa gtttacctca gttagccttg ctggctggaa tgcctcaggc
    22381 accaaaccaa tatgacccct attcacatcc agaagcagcc caagaccgcc gaaacttggt
    22441 cttatctgaa atgaaaaatc aaggctacat ctctgctgaa cagtatgaga aagcagtcaa
    22501 tacaccaatt actgatggac tacaaagtct caaatcagca agtaattacc ctgcttacat
    22561 ggataattac ctcaaggaag tcatcaatca agttgaagaa gaaacaggct ataacctgct
    22621 cacaactggg atggatgtct acacaaatgt agaccaagaa gctcaaaaac atctgtggga
    22681 tatttacaat acagacgaat acgttgccta tccagacgat gaattgcaag tcgcttctac
    22741 cattgttgat gtttctaacg gtaaagtcat tgcccagcta ggagcacgcc atcagtcaag
    22801 taatgtttcc ttcggaatta accaagcagt agaaacaaac cgcgactggg gatcaactat
    22861 gaaaccgatc acagactatg ctcctgcctt ggagtacggt atctacgatt caactgctac
    22921 tatcgttcac gatgagccct ataactaccc tgggacaaat acccctgttt ataactggga
    22981 taggggctac tttggcaaca tcaccttgca atacgccctg caacaatcgc gaaacgtccc
    23041 agccgtggaa actctaaaca aggtcggact caaccgcgcc aagactttcc taaatggtct
    23101 cggaatcgac tacccaagta ttcactactc aaatgccatt tcaagtaaca caaccgaatc
    23161 agacaaaaaa tatggagcaa gtagtgaaaa gatggctgct gcttacgctg cctttgcaaa
    23221 tggtggaact tactataaac caatgtatat ccataaagtc gtctttagtg atgggagtga
    23281 aaaagagttc tctaatgtcg gaactcgtgc catgaaggaa acgacagcct atatgatgac
    23341 cgacatgatg aaaacagtct tgagttatgg aactggacga aatgcctatc ttgcttggct
    23401 ccctcaggct ggtaaaacag gaacctctaa ctatacagac gaggaaattg aaaaccacat
    23461 caagacctct caatttgtag cacctgatga actatttgct ggctatacgc gtaaatattc
    23521 aatggctgta tggacaggct attctaaccg tctgacacca cttgtaggca atggccttac
    23581 ggtcgctgcc aaagtttacc gctctatgat gacctatctg tctgaaggaa gcaatccaga
    23641 ggattggaat ataccagagg ggctctacag aaatggagaa ttcgtattta aaaatggtgc
    23701 tcgttctacg tggaactcac ctgctccaca acaaccccca tcaactgaaa gttcaagctc
    23761 atcatcagat agttcaactt cacagtctag ctcaaccact ccaagcacaa ataatagtac
    23821 gactaccaat cctaacaata atacgcaaca atcaaataca acccctgatc aacaaaatca
    23881 gaatcctcaa ccagcacaac cataacattt atcacccaga tttttctggg tgattttgtc
    23941 atagcactat tgtatttatg tgcttaacaa aagcctgaga agacatcttc tcaggctttt
    24001 tctgtactat aaacctcatt ggaaaagata gagactgctt ccgtaatctt gttaagaggt
    24061 tttactaaat actagtcttt cttcgttttt actacaaaga gagcagatag ggctagacta
    24121 acacttgcta ggaagagggc tgtgtcagat tgactctcac ctgttgctgg caagatttgt
    24181 tcaggtgttt ctggtctatc tatttcttgc ttataatctg gcgtcttacc ttgattggaa
    24241 actaaagagc taagtttaaa ctcaggtttc tctacagttg gagcatctgg tttcgtagca
    24301 gaatctttta attggttgag ataatctgca aagtattcca ccattctttc cgtcaagaga
    24361 tggttatccg tcgcatattt catgcttgcc tgaacgctag ctacttcctc ttgattgtct
    24421 ttgtctgtta atttctgagc cttagccaaa gctgcttcat agcctgacaa gtcaagaggt
    24481 gtttctgcta cctgtggacg agtaaagata agttccgctg cagattggta tttatcgcca
    24541 ccatctccgt aagtcttagt acctgtaagg acaattttct tagccttaat tgtcttacca
    24601 aaatcaatgt cttttggctt attgttatct ggccaatcag ttgcagtaaa ggtatgctcc
    24661 ttgccagact catctgtcac aacaagtttc acatcacgca agttaccatt tgaacctgat
    24721 ccacgtggaa cataacgaag tccagtgatt tcagttggtt ctttcaagac catggttgca
    24781 ggcttgccta catctccacc attccaagat gtatgccata gactagacac attgccatca
    24841 aaggcatttg caagaccttc ttgagcctga gcaggagctg taagacttgc aaagtcatct
    24901 gctaccaaag ccgttttctt ctgaaccaaa gcattcttca aagcttcaat cttggcaatc
    24961 tctgcacgcg cttcttccac actgatatca tcatcggcct gactgaggtt aaagaccgcc
    25021 tctttcaaag catccataga ctctttggtg tagttagtca tggcaaccgt tggcaagtag
    25081 ttcttcagag cattttctgt caacatctta cctgttaggg taatttcttc gatttgaaga
    25141 ttatccatca tgaagtcgtt ataaccacgg aagttggcat ttccaccaga atcaccacga
    25201 gtattacttg catttccagt tgagtagata cctacccaag tatcgcctgt ttctgcacct
    25261 gtcacaagga aggttgcctt cttggctttc ttagaatctg tccaagtatt tggcaattca
    25321 tgcatttcca agttgcttgc ttgagtacca cgacgacctg actggaattc tcccttaccg
    25381 actacaaaag cataggtatt gtctgatcct gcttcgtatt caaaggttac acggtaggtc
    25441 ttacctgctt caaaacggaa gttttgtggg atggtttggt aaaccaagtt acgacggctc
    25501 actagtccat ttgtcttgag tgaccaattt ccttcgataa catcatcgac tttcttacca
    25561 ttccaaccac gttgtgtata tggattgtgt ttttcagaca agtgagtgcg gttatcttca
    25621 acaccttcga caccacccac tacaaatggg aagataccct gagcaacatt ttcaaagtct
    25681 tgcttgaagg tgcctttacc tgtatcatgc ttgtctccgt acatgcttga attgttttca
    25741 aaggtacgaa tttcatcaaa gtaagttgct tgatcaccag cttcacgact caatgtcaga
    25801 gtaacatttg atacgtccga tccagttgta aagaaggcgt acatgttttg gaagtaactt
    25861 gtatcgtcaa ctgtagcatt gttacgacgt gtattgtggg cgtaggcttt tacatagttg
    25921 agggcgagag acttattggt ataagtagtc acttcttttt caccagtatt tacagtgacg
    25981 ctcgccttgg cattactacg gttatcgaca ccgacataaa cggcatactt ggtatttggt
    26041 ttcaagccag ttaatttctg agtgagacta actttttctt tgtttccttg aatacgaagc
    26101 atatcgtttg ccccttgaga cttgacaatt tctgccttag aagcatcgcc tgaaatggtc
    26161 caatgtttca aggtaccact gttaaatcct tggtcataga tgtgcatgcc ttcgctccat
    26221 gacatttcag gattggtttg tttcgaacga tagagaacgt atggttgatt tgctagaaga
    26281 tctagagtaa ttttaccatc ttttacagtt agttcttgct cttctgtctt accttggtca
    26341 gttagcttgt aaaggtaaac cttgctcttt gcccaatcgc ttggaagggt ccaagttgtt
    26401 gcaccggcct gcgtattgaa gtagtacatc ttttccttat cagtagaaag tttcttacca
    26461 tttgcatccc agttccaagg agtcaagtaa gctgaaccat cttggatgac acgtccgttg
    26521 agcgttactg tacgttcgcg atattgtgga ctattgacat catttgactt acgagttaca
    26581 actactttat tattgtcagc atctaccaat tccactcgca tttctggagt ccatttatag
    26641 gtgctaccgt tatcggtcat agtcaccggt gtaccatttt cccatttgct tacagtgaag
    26701 tgttggaaat acttggtcat gacgtcatgg gcaaataagt tggttacata gccattgtag
    26761 tcacttcttc cttgccagcc ttcaaagtct ttcatgctgt agccacctag cagtggatag
    26821 ttggctgcac caccataact tctgtagtcc ccaacccaag catctttttg gtggttacgg
    26881 ataaagcggg tgatggcact gttgatacct ttattggtgt agccaccgta ggtcaagtca
    26941 gctgcccagt gatggaaggt agagtcgtac tcaccaccat ggccccactc gatcgcaaag
    27001 cgccagcctt gtttgttaat ttctttagca agaacgtggg tagcccaggc accgttatca
    27061 cctgattgac cattacccca aacgtccaca tagataaagt cgagaccgtc accaagtttt
    27121 ttcttcaaat cttcccaacg tgccaaacga ccatgagcta ggtcataggc agcatcaatg
    27181 ttgatacctt gatctagcca gttccaacca tagctatagc ttccatctgg attcttacgg
    27241 agaatttttt cattgaagta tttagactca ggataagttt ctgaagcgtt aacgtggata
    27301 cctagatgag ctccatattt cttagccttc tcaattaggg tcttgaagtc ttcgacacca
    27361 ccgatacgct taccaatatc agcatagttc aagtgaccag agtcatggcc ttcgctacca
    27421 tatcctttaa ggagaacacc ttgcccaaga ccatctgtgt ggagattgat tttcttgata
    27481 ccatccaagg tcataaggaa tgggttttgt gcttgagaac caaagttcat cgcgatacgg
    27541 taagctgtga tatccttaac tttttcccaa ccttgaggat tgttcataat gctacgataa
    27601 gcaatggcac catcttgcca atcaactttc ttgtctgcat tggcatcttc agtgataaca
    27661 accttagcac ttggaagttc cttcgtgtat tctgggaaaa caatgccctt ataagctttt
    27721 tcccattgcc attcagagct atggattcct acatagttgg catttccgac tgtttcttta
    27781 taagctgtca aacgagtcca gtcattcgaa ccaccaccat agctattttg agagttactc
    27841 caaacaccag cagcaagctt atctgtagaa acaaatccat acatgtaacc cttagccaaa
    27901 tccttcattg gattggttac atcgatatga tcatctccgc tgacatgcgt attgtttgac
    27961 atggttgccc catcaaactt agcaccagtt tgatcactag aaacagagac taaagcattg
    28021 ccgaggaaac taatagaaga aagtagtttt ctttcgtcat caatcttttg acctggagtg
    28081 acttgattgt ggttgacaat cttggtcaca tcaaagtgca attgattgtc cacaacttgc
    28141 aagcgtactg ttatttccgc attgattaag tgagcatcat cgcgaatctt catcaagtac
    28201 tctgctgttg tctcattgat tttcttataa gtgacttcag gggtgattcg gtggttattg
    28261 ataaagactt ggttgaactg ttgcacctgt cctggcaaag tatgtccatt caagctgtat
    28321 tccttgacac gagggaaggc ttggtcaatc actgctttga ggaccttaga ctgaatcgtg
    28381 tcataagtca ccttgctatc atcaacttca ggacctgttt ctttttcagc aggggtatcc
    28441 tctgttttta ccccctcttg gttatccgtt ttaacgctaa caactgttcg ctcatcgtca
    28501 taagagcccg ccttgagaag aatcttcttc tcatttttaa gatggtcatt gaccgcagct
    28561 ggtagagtca ctgtgtcaaa gagattgaca tcgttattgc tggcatttag ctgaccgtct
    28621 gacttgagag tgatagagag acggtttgtt gatcctgttt caggagcagc aacacgacta
    28681 cctctatacc aagtgctagt tgttggagat ttatactccc agaaccagcc atccttgtca
    28741 taaccgacaa aaacattatt attggtatct ttaaatttca agaagacacc aaagcgtgat
    28801 ttgccctttt cagaatcttc tttgaaggtt aaatcaacag ttgcatttcc attggcatca
    28861 acggtcaagc ccttcttttc aaacagggct tgtttgcctg cgttatcatt ttgagcagtt
    28921 gaggatagtt ggttgtagcg gacacctttt tcttctcgga tagtgactgt tccttgttgt
    28981 tcttttttct ctaccgtttg ccattcaggg gttaccgtct taggtgtttc aggtttagca
    29041 gctgcaggtt tatcctcttt tggtttttct tcagccggtt tttctttttc aagtgctaat
    29101 agttcttctt ctgtcttagg tccatctgta acttctggag aaccttggtc ttctcccacc
    29161 ttaggcgctt caaaatcacg ctcatcattc tcttttgctg ttgcaagagc agtagctaaa
    29221 tcagctggta aattcgccgt agaaccagac tgcacgctat ctgcaagaac cggacttgtc
    29281 ccaaagaatg cagctccaat cataacagaa gcaacaccta atgaaaattt ccgaatacta
    29341 tatttacaac gtttttcaaa taatccttta ttcataaact tctaaatcct ttcatttttg
    29401 ggcaagcgtt tacatttcct ttttaaaaaa tactccttcc tctgaaagtg caatgcttaa
    29461 ccctgataaa taaaaaataa ttatactttt aatataacaa ataataactg ataaagcaat
    29521 aggaaacaag taaaattaac aaaaattttc tctattctga caaaaagagg ctccaacttt
    29581 tgggggtcag tacatttaga atctttcttt acaaatgtaa aaaaggattc agcattcaag
    29641 agaaatacta aatcctttct tatattttgc aacagttatt tcacatgttt tgcgagatct
    29701 tcttgagcct ttttattaga ctcttctttt tctttcatcc atttttcctg agctttttgg
    29761 tattcatcta cagtgactgc cttgtcttga agttccaagt atttatataa gattggttca
    29821 cttgtacctt tatttcctga caatgcaaat ggtattgtaa atggtaccat cttagacaag
    29881 attgggcgcc ctgtacgaga tgtagttgga ataatcaaag cactatctgt caaccaagct
    29941 tgggctgcag cgtatttatc atagcgttta gcaacatctg tagcctcatc accagcctca
    30001 gtaaccaatt tttcgtagtc atatagacct acttttttag cagctacatt atcttcccct
    30061 gagtcaaacc ctaaatatgt tttagtactt tctcctacag atggtttgat gatatcaagg
    30121 taagttgatg gatcggcaaa gtctggaccc caaccgacat tatctgataa atcccagtct
    30181 tcgccagcag cattttcagc aaaatatgta atattgttta cttcgtcttt ttgtagttgt
    30241 tggatatcaa taatgacatt atcagctcct aaagttgctt ccaaggattg tttcatagat
    30301 tggacgcgct gaacttttgt agttgctgtc tggtcaactg gcatatccaa atgaattggg
    30361 aatgtcacac cttctgcttg taaggctgat ttagctttag caaattcagc cttggctttt
    30421 tctggattgt aaagaccatc ctgagaatct gcaagattaa catccttcca ttcatcccca
    30481 taagtgacca atttctcttt gaccatatcg ccaaagtttt taccatctgc ttgaacaaat
    30541 gttggtggca caaagagatt acgcaagatt ttacttgctc cagtttgtcc attcaactga
    30601 gaggcatagg ctgtacgatc aaaaccaaag gcaatagcct gacggaaatc cttgtttaag
    30661 agagcctttt tagtcgatgc cttttgttcg tcgctggtct tagatgtgta tttataggac
    30721 tgacggtcaa tatttgtacc aactagatac gtaatagagt cttgttgagt atagacaata
    30781 ttgtccttca tactcttctc aagctctgcg aaacttgcac ttgttggata gagacgagct
    30841 gctgtaaggc taccatcttt aaagttttct gcaggtttgc tggtatcttg accatcccag
    30901 aatgacaatt taactttgtc aatatgcaca ttgtccttat cccagtagtt cggatttttc
    30961 gcaaattcaa cagaagattt ggtcacaatg gatttcaaca aataaggacc gttatacaag
    31021 agactacttg gatccgtagc tttggcaaaa tcatcccctt ttgaattcaa aaactcttca
    31081 ttaactggcg caagcacacc catggttgtc ttagaattcc agaagctttc tggtttgttc
    31141 aaagtgtact gaactgtctg ttcatccaga accttaattc ctacttgtga gaaatctttg
    31201 atttcccctt ttacataggc atccaaccct ttgattgatt cttgaacaag gtaaagagca
    31261 tctgattttt tatcagcagc atattttagt cctgttacaa agtcttgagc tttgactgcc
    31321 gcgtattctt ccccttcaga agtataccat tttgcatcct tacggatagt ataagtgtaa
    31381 gtcaatccat ccttggatac agaccaatcc tcagccatag acggcacaaa gttcccgtag
    31441 cgatcatttt ctagcaaacc atcaaccacg ttactggtaa tatttgctgt cgcagcctta
    31501 gcagttgtca aatagttgag gttatcaggg tctgtctcat aaatgtatga gaatgtcttc
    31561 tcacctttag tgcttgaacc tgatccagag catgcagcta aagtagtcgc cgccaataat
    31621 gtcacgcccg caagggcaaa tagttttgaa cttttcatca ttttctcctt taaaactttc
    31681 tctccattat aaactctttt cagaaagatg tcaacagaat tttcagaatt tttgtgttaa
    31741 tttggtatat tctgaaaaga caaaaaaaga gaagacagtg attgtcctct ccagatgtta
    31801 gttgacaaag agccacatta taaatctaaa ataatttatt ttaatttaat atacttttgt
    31861 ggtaataaaa caatattcaa aaatggaatt tttttcaata tataagttgc tagtatggat
    31921 aaataccagc ctagcacaat cccaacaaca acgtgaagaa aaactgagtt tattcccact
    31981 ttcaacatta gaatccgaat catgctacaa attggtgcat gaagaatata gatccctaaa
    32041 ctatctttcc cgtatttagt gaaatatagg aaagttttag gaaaattttt ctccatttta
    32101 ggaaaaatcg caaaagcaac taatacacag acaataaaag caatccccca caaacctggg
    32161 ttaatatagc ttacataatc cttcttagac cctacttcat aaaacaagaa ccaagcgaac
    32221 atataaataa aatcaaataa cacaaagaaa aaaagaaatt ttttcaaatt aattttatca
    32281 aagtgaattt cactcaatac actgccaaga aagaaacaaa tcccccaaac caacgtccgt
    32341 tgcacaataa aaatatcagt ttgaataaat agtgtgaaaa tataggcaaa aacactaact
    32401 aacaaaaggg acttacgatt tttgaaaaca acagatagta aaccataaac tatcaatatc
    32461 gaccataacg tatatagata ccatgaaact cccagaggaa atttatagat attcaatagg
    32521 ttaaatatca tagtgggtac tcggacagag gcacccgcaa ccttttgaat acaaaagtga
    32581 ataattgaat aaaaaatata tagtatgcct aaaatatccg aagcgtttac gatgatttcg
    32641 ataggttgtt gaaaacattt taaacgtttt tactttggca aggatgttct caaccttgct
    32701 tctatcctta gacagtgcat ggttataggc tttatcttca gctattagcg gcttgagttt
    32761 gctggattta cgtggaatt
//
LOCUS       DBEJAQ010000025        31861 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.025, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000025 DBEJAQ010000000
VERSION     DBEJAQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 31861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.025"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(21..956)
                     /gene="birA"
                     /locus_tag="ACL297_001186"
     CDS             complement(21..956)
                     /gene="birA"
                     /locus_tag="ACL297_001186"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGR0071453.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(953..1813)
                     /locus_tag="ACL297_001187"
     CDS             complement(953..1813)
                     /locus_tag="ACL297_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGR0071454.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1921..4083
                     /locus_tag="ACL297_001188"
     CDS             1921..4083
                     /locus_tag="ACL297_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGR0071455.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNVPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4175..5434
                     /locus_tag="ACL297_001189"
     CDS             4175..5434
                     /locus_tag="ACL297_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGR0071456.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5448..6314
                     /locus_tag="ACL297_001190"
     CDS             5448..6314
                     /locus_tag="ACL297_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGR0071457.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6328..7161
                     /locus_tag="ACL297_001191"
     CDS             6328..7161
                     /locus_tag="ACL297_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGR0071458.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7168..7335
                     /locus_tag="ACL297_001192"
     CDS             7168..7335
                     /locus_tag="ACL297_001192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071459.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKVKLTWFEEI
                     FEEYKKSSWNL"
     gene            7519..8961
                     /gene="gtfA"
                     /locus_tag="ACL297_001193"
     CDS             7519..8961
                     /gene="gtfA"
                     /locus_tag="ACL297_001193"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HGR0071460.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9099..10292
                     /locus_tag="ACL297_001194"
     CDS             9099..10292
                     /locus_tag="ACL297_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGR0071461.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10978..12957
                     /locus_tag="ACL297_001195"
     CDS             10978..12957
                     /locus_tag="ACL297_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0071462.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKTSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13024..14520
                     /locus_tag="ACL297_001196"
     CDS             13024..14520
                     /locus_tag="ACL297_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071463.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14520..15446
                     /gene="oppC"
                     /locus_tag="ACL297_001197"
     CDS             14520..15446
                     /gene="oppC"
                     /locus_tag="ACL297_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGR0071464.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15455..16522
                     /locus_tag="ACL297_001198"
     CDS             15455..16522
                     /locus_tag="ACL297_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071465.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16533..17459
                     /locus_tag="ACL297_001199"
     CDS             16533..17459
                     /locus_tag="ACL297_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0071466.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            17949..18886
                     /locus_tag="ACL297_001200"
                     /pseudo
     CDS             17949..18886
                     /locus_tag="ACL297_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            18898..19791
                     /locus_tag="ACL297_001201"
     CDS             18898..19791
                     /locus_tag="ACL297_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071467.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            19804..20607
                     /locus_tag="ACL297_001202"
     CDS             19804..20607
                     /locus_tag="ACL297_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071468.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(20640..21350)
                     /gene="treR"
                     /locus_tag="ACL297_001203"
     CDS             complement(20640..21350)
                     /gene="treR"
                     /locus_tag="ACL297_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HGR0071469.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIREQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21535..23502
                     /gene="treP"
                     /locus_tag="ACL297_001204"
     CDS             21535..23502
                     /gene="treP"
                     /locus_tag="ACL297_001204"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HGR0071470.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23680..25305
                     /gene="treC"
                     /locus_tag="ACL297_001205"
     CDS             23680..25305
                     /gene="treC"
                     /locus_tag="ACL297_001205"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGR0071471.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25561..25809
                     /locus_tag="ACL297_001206"
     CDS             25561..25809
                     /locus_tag="ACL297_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HGR0071472.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26016..26810
                     /gene="racE"
                     /locus_tag="ACL297_001207"
     CDS             26016..26810
                     /gene="racE"
                     /locus_tag="ACL297_001207"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGR0071473.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            26807..27778
                     /locus_tag="ACL297_001208"
     CDS             26807..27778
                     /locus_tag="ACL297_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HGR0071474.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27754..28275
                     /locus_tag="ACL297_001209"
     CDS             27754..28275
                     /locus_tag="ACL297_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGR0071475.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28272..28733
                     /gene="cbpB"
                     /locus_tag="ACL297_001210"
     CDS             28272..28733
                     /gene="cbpB"
                     /locus_tag="ACL297_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HGR0071476.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28730..29464
                     /gene="xerD"
                     /locus_tag="ACL297_001211"
     CDS             28730..29464
                     /gene="xerD"
                     /locus_tag="ACL297_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGR0071477.1"
                     /translation="MRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETSLK
                     IYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEILYL
                     DSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVTIP
                     TALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFILRQ
                     IENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29464..30192
                     /locus_tag="ACL297_001212"
     CDS             29464..30192
                     /locus_tag="ACL297_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGR0071478.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30211..30780
                     /gene="scpB"
                     /locus_tag="ACL297_001213"
     CDS             30211..30780
                     /gene="scpB"
                     /locus_tag="ACL297_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGR0071479.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            30767..31489
                     /locus_tag="ACL297_001214"
     CDS             30767..31489
                     /locus_tag="ACL297_001214"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGR0071480.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31489..31731
                     /gene="yidD"
                     /locus_tag="ACL297_001215"
     CDS             31489..31731
                     /gene="yidD"
                     /locus_tag="ACL297_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGR0071481.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
BASE COUNT         9236 a         5919 c         7050 g         9656 t
ORIGIN      
        1 tatattataa ttgtgttatt ttacttccaa ctattgagag aaatttcgcc actatttagc
       61 cagatttctt ttccgttatc acattgaact aaaagttttc cattttctga gatgtcttta
      121 gcaagtccct tgtagtcttt ttgctgtagt gtgaaagtga cttcttttcc tagaatgaat
      181 gactgttttt tgtataggta taatagctct tctgctggtg tttcgaagaa agcacgccag
      241 atttctatga tcaattcatt ccttgttata ggagctgtag ctttaaataa gctggcagct
      301 ttttctttta attcctgagg gaagtcttta atagtgaaat tgatacctac tccaataatg
      361 atatctgtga ctaagccagt ttctacagag gtcattgctt cagtaaggat tcctccaatt
      421 ttatgattgt ttagatagat atcattgacc cattttatgt cgacatctat taaagttagg
      481 ttcttaatag ctttgtagac agctccagct acaagtagtg tgtaggatgg taatttgtca
      541 taggggagat ttggtttaag atggagtgtc atataaatac caccttgtgg tgagtagaag
      601 gaacgttgaa aacgtcctcg gcctgctgtt tgataggaag ctagatagag ggtatttgct
      661 tcatggccta aatcaattgc ttcttttgca tctagttgtg ttgattttgt ttcgggttta
      721 aagctgactt taattggaag attttcttct agaatctctg gaagaataag gtcaccattc
      781 attagtttat atcctctatt tttgatacta tcaatttcaa tgccttcttg ttctagtcgc
      841 ttgatagctt tccaaattga tgttcggctt agggatagtt tttctgcgat tttttctccg
      901 ctgatatagt cggtttcttt agataggatt tggtagacag cttggtagga tttcataatg
      961 ttgcctttct tactagttgg tattgagagt attcttttct tgtatgactt ggagtctgat
     1021 taaagtattg tttataagct ttcgaaaaat ggagtggatc tgaaaaacct accgagtatg
     1081 caattacctt gatggactct tgggtatttt cgagaagttg tctagctcgg tgcattcgaa
     1141 cgtagagtag gtattctttg ggtgataagg tattaaattc tttgaatacg cttgataagt
     1201 agcttctgtg aacggatagt tcttttgcta aatcttgaat tgtaagtgat tgaggatagt
     1261 ggctatcaat taatcgtttg cattcaagat agagttggtg ggttgatgaa atattctttt
     1321 ttttctgatt gggagcaata gttcccagat gaaacatcag ttcatgaagt tgtcccatga
     1381 tatggagttg agctaattca cttgattttg taatctgagc gaagcggaca atgtctgaga
     1441 tgagttttgc agtagtctgg gtatgacaag tttcagattg gatgagatag gattgatcag
     1501 aaatttggga aagagcaaaa taatcagggg ctttccctcc agtgattcct aaccagtagt
     1561 aggcccaagg ttctttacta tctgcttgat aaaaggttag ttcctctggt tttaatagaa
     1621 agaaatctcc ttcttttaaa tcaacaattt tacccttgta atgaaatttt ccttgtcctt
     1681 tagtaatgta atgtagaacg tatgtatcac gaatggctgg accaaaagag taattaggtg
     1741 tgcattcctc atatccataa aagcttaggg caaggtcgat tgttccagtc tggtattctg
     1801 aaaaaactaa catgtgctac ctcctaccta acattttacc atattcttga gacatttttc
     1861 tattttggaa agcgatttca ggtgataaaa tataatcaat aaagtgatga ggtgaagtaa
     1921 atgggagtta ggatagagaa taatctattt tatgttgaga gtaaaaatct aagtttgatt
     1981 attgaaaatc gaaatggcta cttacttttg aaacatttag gaaagactat taagaactat
     2041 aaaggttcca atagtgttta tgaacgagac catgcctttt caggaaatcc aacggctact
     2101 aatcgaacct ttagtttaga tactcagcga cagatttttg gacaacatgg cttaggagat
     2161 tttaggaaac caactataca ggttcagcat agtgtaactg aagtaacaga ctttcgattt
     2221 gtagaagcaa agattttaaa aggtcagaat gttccacagg gcttaccttc tccacatagc
     2281 atggacgata cagagactct tgtcttaatg ttagaagatt ctaaggctca acttagtctg
     2341 actttgtatt atactacttt taataatgat gcgactattg ctagctacag taaattagat
     2401 aataatagta atcaggaagt tgtcatccat aaagattttt cttttatggc tgattttcca
     2461 gctgcagatt acgaaatagt aactctgcag ggtgcttatg ctcgtgaaaa gactgttaga
     2521 cgtcaacagg tagaacaagg aatcttttcg attagttcaa accgaggtgc ttctggtcat
     2581 gctcaaacac cagctcttct actatgcgaa caaggagtca cagaggatgc tgggaatgtg
     2641 tttgctattc aactaatgta tagtggcaac tttgaagctt ttgttcaaaa aaatcaattg
     2701 aatgaagttc gggtggctat tggcattaat ccagaaaact tttcttggaa gttagcccct
     2761 gaggaatact ttgaaacacc ggtagcttta gtgactcatt cagatcaggg attaactggt
     2821 attagtcatg aaagtcagaa ttttgtactg aagcacatta tgctaagtga attttctaaa
     2881 aaagaacgtc caattctaat caataactgg gaagctactt actttgactt tcagagagaa
     2941 aaactgttag agttagcaga tgaagctaag aaagttggca ttgaactttt tgtattagat
     3001 gatggttggt ttggcaatcg ttttgatgat aatcgtgctt taggtgattg ggttgttaat
     3061 gaggaaaaac tgggtggaag tctagaaagt ctgatttcag ctatccatga aagaggtttg
     3121 cagtttggac tttggttaga acccgaaatg atttctgtag atagtgattt gtatcgtcaa
     3181 catcctgact gggctattca ggttcctgat tatgagcata cttattctcg gaatcaatta
     3241 gtacttaatc ttgccaatcc tcaggtagta gaatacttga aaagtgtctt agatcaactc
     3301 ctatcttatc atgagattga ttacattaaa tgggatatga accgcaatat cactaagcta
     3361 gggaatggat taacttatct agagacacag atgcaatctc atcagtacat gctggggctt
     3421 tacgaactcg tttcttatct gacagagaag cacagccata ttctctttga gtcctgctct
     3481 ggtggtggtg gacgaaatga tcttggtatg atacgctatt tcccacaagt ctgggctagt
     3541 gataatactg atgccattgc acgtttacca attcaatacg gttcatccta tctctatcca
     3601 accatttcta tgggggctca tgtgtcagca gtaccgaatc atcagatggg acgaatgaca
     3661 ccattagaaa cacgtggcca tgtagcaatg atgggaaatt tgggctatga gcttgatttg
     3721 acaaatttat cagatgaaga gaaagctacg attgctaatc aggtgaactt gtataaagaa
     3781 ttacgaccag tagttcagtt aggacagcag tatagactaa ttaatcctga tactgcatcc
     3841 aatgaagcag ctgtacaatt taattacgga aatcaaacga ttgtaaccta cgttcgcgtt
     3901 ttatctgttg tagagaccat ggaaacaact ttaaaattaa aagatttgga tgaagaggga
     3961 ctatataaat tacaggaaaa tggcgaagtt tactcaggtg cagaactaat gtatgcgggc
     4021 ttaactgtta tcttatccca aggagatttt ttgagtagac agtatatttt tagaaaacta
     4081 tgatatgtac tgaccccaaa agttagattt attctgtcta acttttgggg tgcagttcaa
     4141 taagggtgta taaatttata aaggaggctt tctaatggaa tggtataaaa aaatcggact
     4201 tcttgcaact acaggtttag ctttgtttgg gctcggcgct tgctccaact atggtaaatc
     4261 tgcggatggc acagtgacca tcgagtattt caaccagaaa aaagaaatga ccaaaacctt
     4321 ggaagaaatc actcgtgatt ttgagaagga aaaccctaag atcaaggtca aagtcgtcaa
     4381 tgtaccaaat gctggtgaag tattgaagac acgcgttctc gcaggagatg tgcctgatgt
     4441 ggtcaatatt tacccacagt ccatcgaact gcaagaatgg gcaaaagcag gtgtttttga
     4501 agatttgagc aacaaagact acctgaaacg cgtgaaaaat gcctacgctg aaaaatatgc
     4561 tgtaaacgaa aaagtttata acgttccttt tacagctaat gcttatggaa tttactacaa
     4621 caaagataaa ttcgaagaac tgggcttgaa ggttcctgaa acctgggatg aatttgaaca
     4681 gttagtcaaa gatatcgttg ctaaaggaca aacaccattt ggaattgcag gtgcagatgc
     4741 ctggacactc aatggttaca atcaattagc ctttgcgaca gcaacaggtg gaggaaaaga
     4801 agcaaatcaa taccttcgtt attctcaacc aaatgccatt aaattgtcgg atccgattat
     4861 gaaagatgac atcaaggtca tggacatcct tcgcatcaat ggctctaagc aaaagaactg
     4921 ggaaggtgct ggctataccg atgttatcgg agccttcgca cgtggggatg tcctcatgac
     4981 accaaatggg tcttgggcga tcacagcgat taatgaacaa aaaccgaact ttaagattgg
     5041 gaccttcatg attccaggaa aagaaaaagg acaaagctta accgttggtg cgggagactt
     5101 ggcatggtct atctcagcca ccaccaaaca tccaaaagaa gccaatgcct ttgtggaata
     5161 tatgacccgt ccagaagtca tgcaaaaata ctacgatgtg gacggatctc caacagcgat
     5221 cgaaggggtc aaacaagcag gagaagattc accgcttgct ggtatgaccg aatatgcctt
     5281 tacggatcgt cacttggtct ggttgcaaca atactggacc agtgaagcag acttccatac
     5341 cttgaccatg aactatgtct tgaccggtga taaacaaggc atggtcaatg atttgaatgc
     5401 cttctttaac ccgatgaaag cggatgtgga ttaggagtag agagactatg aaaaaagtat
     5461 tacaaaaata ttgggcatgg gcttttgtgg tcatccccct cttgttacaa gcaattttct
     5521 tctatgtgcc gatgtttcaa ggagcctttt acagttttac caactggaca ggattgactt
     5581 acaactacaa atttgtcggc ttaaacaact ttaagctcct cttcatggat ccaaaattca
     5641 tgaatgcgat tggctttacc gcaatcatta cgattgccat ggtggttggt gagattgcac
     5701 tcgggatctt cattgcgcgt gtcttgaact ctaaaatcaa aggccaaacc ttcttccgtg
     5761 cttggttctt cttcccagct gttttatctg gtttgacagt ggctttgatc ttcaagcaag
     5821 tcttcaacta cggtcttcca gcgattggga atgcccttca tattgaattt ctccaaacca
     5881 gtcttttagg gactaagtgg ggagcaatct ttgcggctgt ctttgtcctt ctttggcaag
     5941 gggtggctat gcccatcatc atcttcctag ctggtttgca atctattcca actgagatta
     6001 cagaggcagc aaggattgat ggtgcgacta gcaagcaagt tttctggaac attgaattgc
     6061 cttacttgct accaagtgtc tctatggtct ttatcctagc cctaaaaggt gggctgactg
     6121 cctttgacca agtctttgcc atgaccggtg gtggtccaaa caatgccaca acctcacttg
     6181 ggctcttggt ttataactat gcctttaaaa acaaccaatt cggttatgcc aatgccattg
     6241 ccgtaatctt gttcctctta attgtagtga tttcgatcat ccaattgaga gtatctaaga
     6301 aatttgaaat ttaagaggag aagcatgatg aaacaagatg aaagaaaagc cctgattggc
     6361 aaatacattc tattgattct aggatcggtt ctgattttag tgccgctcct tgctaccctc
     6421 tttagttcct tcaaacccac taaggatatt gtagataatt tctttggctt tccaaccaac
     6481 ttcacatggg acaactttag ccgtctctta gctgatggga ttggaggcta ttattggaac
     6541 tctgtcgtca tcactgtctt gtctttactt gcagtaatga tctttatccc tatggcagcc
     6601 tactccatcg ctcgcaatat gagtaaaaga aaagtcttta ccatcatgta taccctctta
     6661 atcctcggaa tcttcgtacc tttccaagtc atcatgattc cgattacggt tatgatgagt
     6721 aaactcggtt tggctaatac ctttggtttg atcttgctct acttgaccta tgcgattcca
     6781 cagaccctct ttctctatgt tggctatatc aaaatctcga ttccagaaag tctggatgaa
     6841 gcagcagaga tcgatggggc taatcaattt acaacctatt tccgcatcat cttcccaatg
     6901 atgaaaccga tgcatgcgac aaccatgatc atcaatgccc tttggttctg gaatgacttc
     6961 atgttgccac tccttgtctt gaaccgggat tccaaaatgt ggactctgcc tttgttccaa
     7021 tacaactacg caggccaata tttcaacgac tacggaccaa gctttgcctc ttacgtggtc
     7081 ggcattatca gtatcaccat tgtctatctc ttcttccaac gccatatcat ttcaggaatg
     7141 agcaacgggg cagtgaagta atactcaatg aaaatcaaag agcaaactag gaagctagcc
     7201 gcaggttgct caaaacactg ttttgaggtt gtggatgaaa ctgacgaagt cagtaaccat
     7261 acctacggta aggtgaagct gacgtggttt gaagagattt tcgaagagta taagaagagc
     7321 tcatggaatc tatagtcttt gacacagagg aagagttcag cttgttgata attctatgta
     7381 gaaaacaaga aagtagtact ttgtttatca cagtctatat tagtacgagt ttgatgataa
     7441 atagagattt aaaatgaatt tgatttacga agccagatca tatgagactt ggcttcaatt
     7501 agaaaaagga gagtaatgat gccaattcaa aataaaacca tgttgattac ctattctgat
     7561 agccttggaa ataatcttaa agacttatat gataatttgg aagagcattt tggagatgct
     7621 attggaggag ttcacctttt accatttttc ccatcaacag gtgatcgtgg atttgcgcca
     7681 gttgactacg acgaagtgga ctcagctttt ggtgattggg aggatgttaa gcgtttaggt
     7741 gagaaatatt atcttatgtt tgattttatg attaatcata tttctcgtca atccaagtat
     7801 tataaggact atcaagaaaa acatgaagcc agtgaattta aagatctctt tttaaactgg
     7861 gataagtttt ggccagaaaa ccgtccgaca cagtctgatg tagatttaat ttacaagcgt
     7921 aaggatcgtg caccaaagca agagattgtg tttgaagatg gttcagtgga acatttgtgg
     7981 aatacctttg gtgaggagca gattgatctt gatgtgacca aagaagtaac tatggaattt
     8041 atccgtaaga ccattcagca cttggcaagt aatgggtgtg atttgattcg tctagatgcc
     8101 tttgcttatg cagtgaagaa attggatact aatgatttct ttgtggaacc agatatttgg
     8161 gatttattgg acaaagttcg agatatcgct gctgagtatg ggacagagct tttacctgag
     8221 attcatgaac actattcgat tcagtttaaa atagcagacc atgattacta tgtttatgat
     8281 tttgctcttc caatggtgac actttatact ctttacagtt ccagaacaga gcgtttggct
     8341 aagtggttaa agatgagccc gatgaagcaa tttacgacgc tagataccca tgatgggatt
     8401 ggagtagtag atgtcaagga tatcctgacc gatgaggaga ttgactatgc ttcaaatgaa
     8461 ctctataagg ttggagccaa tgtcaaacgt aagtactcta gtgccgagta taacaactta
     8521 gatatctacc aaatcaattc aacctactat tcagcgcttg gagatgatga tgtcaagtat
     8581 tttctcgctc gtctaattca agcttttgcc ccaggtattc ctcaggttta ctatgtgggt
     8641 ctattagcag gcaagaatga cttgaaatta ttagaagaaa ctaaagaagg tcgaaatatt
     8701 aatcgtcatt actatagcaa cgaggaaata gcaaaagaag tgcaacgacc tgttgtgaag
     8761 gcccttctca atctattttc tttccgtaac cgttcagaag cctttgatct agaagggact
     8821 actgagatag agacaccaac agctcacagc attgtaatca aacgtcaaaa taaagataag
     8881 tccgtaacag cagtagcaga aattgacttg caaaatcaga cttatcgggt aattgagaat
     8941 ggagttgaag taacattttg aagccttgat atggtggata aaatagggtt tttattttgg
     9001 aaaacgtttc ctttgttttc aaattgctaa aaaagtggta caatatagga tagcttacta
     9061 ttatctgaat cagctgattt ggagagaaag gattcatttt gaaatcaata ggctttattg
     9121 aaaagctgaa ggggttgtct agtaaagagc tgattttatt gggaattatc ctgagtatct
     9181 ttttaccctt ttatcttttt gtagttgtat tctgtttata tattatcagt ctgattttta
     9241 caggagacat gaaaagtatt cttcagaaaa tgggggagca ttcgatgctg cttctttttc
     9301 ttggctatag tactgttata tccattcttg cacaaaattg gatgggtctt gtggcttcag
     9361 taggaatgtt tctatttact attttctttt tgcactatca gtcgatttta tcccataaat
     9421 tctttcgatt gattttgcag ttcgtcttgt ttggtagtgt cttgtcagct gcttttgcca
     9481 gtttagaaca tttccaaatt gtgaagaaat ttaactatgc ttttctttcc cccaatatgc
     9541 aggtgtggca tcagaaccgg gcagaagtga ccttctttaa tcctaattat tatggaatta
     9601 tttgttgttt ctgtattatg attgctttct atctgtttac aacgaccaag ttgaattggt
     9661 tgaaagtatt ctgtgtgatt gcaggctttg ttaatctctt tggtttgaac tttactcaaa
     9721 atcgaactgc ctttcctgct attatcgctg gagcaattat ctatctcttt acgactatta
     9781 aaaactggaa ggccttttgg cttagtattg gggtcttcgc gattggtttg agtttcctct
     9841 tttctagtga tttgggagtt cgaatgggta ctttagactc ttctatggaa gaacgcattt
     9901 ctatctggga tgctgggatg gccttgttta agcaaaatcc tttttggggt gaagggccat
     9961 tgacctatat gcactcttat cctcggatac atgctcctta tcatgaacat gcccacagtc
    10021 tttatattga tacgattctg agttacggaa ttgtgggtac cattttatta gttctgtctt
    10081 ctgttgctcc tgttcgcttg atgatggata tgagtcagga gtcggggaaa cgtccgatta
    10141 tcggccttta tctatctttc cttacagtgg ttgctgtgca cggaattttt gacttggctc
    10201 tcttctggat tcagtcaggc tttattttct tgctagttat gtgcagtatt ccattggagc
    10261 atcgaatgtt ggtatcggac atgacggatt aagttttata agtttgaaat cttctacctt
    10321 gggtaggaga ttttttatac gatgggcatg tcgtgcatct gaggtgtaag tcctctgtag
    10381 ggatccacta ccggtgaata caatagcgct ccccaagcct gactatcgtg aggtagcgga
    10441 ttaaaatggt ctggggatag atcgttttaa gtctgacgca ggaaataaga attgtcagag
    10501 aaagcaatag tgaaatcgta ggctatgaat agaaacgtga tagcgaggag catatagtgg
    10561 ataaggtagc gagaaatttt aaagtataaa agatagtctt aatctatata tgtaaatcac
    10621 aaaagtaatt gaattcggaa taaggttggt gtgaaaagac atagtaaact gaatcttgaa
    10681 tactagaatg atacttctac aatattgtta taatttaatt tgaattccaa aatcacattg
    10741 ttccgtccgt atttcatttt actgtatgtc ttcctaaact ccaaagtttc tgcgtcaaat
    10801 ttcctatttt cactgtagtc tttgacactt cgaacgacaa tttgaatcat atattcaaaa
    10861 aaaaatgaaa aatttgtgcc aacaagccta aatattctga aaatttgttt gatttttaat
    10921 ggataatgtg atataatggt tgtcaatcta taattcacat aaacagagga gtagaaaatg
    10981 aaaaaaaatc gtgtatttgc tacagcaggt cttgttttat tagcagcagg tgtacttgca
    11041 gcatgcagtt cttcaaaaac atctgattca tcagccccta aagcttatgg ctatgtttat
    11101 acagcagacc cagaaacctt ggactacctg atttcaagta aaaatagtac aacagtagtg
    11161 acttcaaatg ggattgatgg tttattcact aacgataatt acggtaatct tgctcctgca
    11221 gttgcagagg attgggaagt ctctaaggat ggtttgacct acacttataa gattcgtaaa
    11281 ggggttaaat ggtttacctc tgatggagaa gaatatgcag aggtgacggc taaagatttc
    11341 gtgaacggtt taaaacacgc agcagataaa aaatcagaag ctatgtattt agctgaaaat
    11401 tcggttaaag gcttggcaga ttatctatca ggaacttcaa cagatttttc aacagttggt
    11461 gtcaaggcgg ttgatgatta tacgttacaa tacactttga accagcctga accgttctgg
    11521 aactctaagt tgacctattc tattttctgg cctctgaatg aagaattcga aacatcaaaa
    11581 ggaagcgatt ttgctaaacc aacagatccg acatccttgc tttataatgg tccattcttg
    11641 ttgaaagggt tgactgcaaa atcttctgta gagtttgtaa aaaatgagca atattgggat
    11701 aaagaaaatg tccacctaga tactatcaat ctagcttact atgatggatc agatcaggag
    11761 tcgctagagc gtaacttcac tagtggagct tatagttatg cccgtcttta ccctaccagc
    11821 tccaactatt ctaaggttgc agaagaatac aaggacaata tctattacac acaatcaggc
    11881 tctgggattg ctggtctggg tgtgaatatt gatcgccaaa gttacaacta tacttctaaa
    11941 actacagatt cagagaaagt agctactaag aaggcattgc ttaacaaaga tttccgtcaa
    12001 gccttgaatt ttgctcttga tcgctcagct tactcagctc aaatcaatgg taaagatgga
    12061 gcagctttag cagttcgtaa tttatttgta aaaccagact ttgtttcagc tggtgagaag
    12121 acctttggtg atttagtcgc tgctcaactt cctgcttatg gtgatgagtg gaaaggtgtg
    12181 aatttagctg atgggcagga tggtttattc aatgctgaca aggccaaggc agagtttgcg
    12241 aaagctaaga aagctttaga agcagacggc gttcagtttc ctattcatct ggacgttcca
    12301 gtagaccaag catcaaaaaa ctatatatct cgtattcagt cctttaaaca atctgtagaa
    12361 acagttcttg gtgttgaaaa tgtcgttgtt gatattcaac aaatgacaag tgatgaattc
    12421 cttaatatta cttactatgc tgccaatgct tcatctgagg attgggatgt atcgggagga
    12481 gtttcatggg ggccagacta tcaagaccca tctacttacc tggatatttt aaaaacaact
    12541 agcagtgaaa ctacaaaaac atatttagga tttgataatc caaatagccc ttcagtagtt
    12601 caagttggtt tgaaagaata cgataaatta gttgatgaag ctgccaaaga gacaagcgac
    12661 ttgaatgtcc gttatgaaaa atatgcagcg gctcaagcat ggttgacaga tagttcactc
    12721 tttattcctg ctatggcttc ttctggtgca gcaccagtgc tttcacgaat tgttccattt
    12781 actggagctt ctgcgcaaac aggctctaag gggtcagatg tttacttcaa atatttgaaa
    12841 ttacaagata aagcggtgac taaggaagag tatgaaaaag ctcgtgaaaa atggttgaaa
    12901 gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg caagtcatgt gaagtaaata
    12961 atttctaata gaactttctc tccatgagga gaaggttctt ttgggatttt taaaggaaat
    13021 aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat tggtttcgat tttcttagta
    13081 acgactttga cctacacgat tatctatacc ttggttcctc gaaaattgat tttcaagcag
    13141 gatcctaact ataataaaat tgcgacaacg gctgataaac gtgataacta tgaaaatact
    13201 gtgtttgagc gtatgggcta cattgagtat tacgatacta aagagttgca agaaaaggca
    13261 agtagcatgg attcttctgt aacagtagaa gcaaatgcga ccaataaagc tatttatgaa
    13321 aagtacatca atcaattagg tcatggttgg actttgggag aatttactga aagtggtcaa
    13381 ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt ttcacttcta tgctaacttg
    13441 attgacattg accatacaaa taaaatccaa gaccctgaaa atccagactt gaaacgctac
    13501 cttcgttttg aaaacgatcc agctatcgga tggtcattgg tcggttcagg aactaaacat
    13561 aaatatctct tgtactttaa cagtcagttc ccatttgttc atcaaaactt tgtgaacttg
    13621 aatttaggtg actcttaccc aacctatgct aatacaccag ttcttcaggt tattactcaa
    13681 ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa caggtaagaa aacgtcttct
    13741 gtaaatattt actcaagaac ctacaagtca cctagtcagg ctgactctcg tgaagtagct
    13801 agctatggga aagatgatcc ttatacagcg actgaaagta attaccaata tccatctatg
    13861 attgtcagct ctgctattac tggtttgatt ggtttggttc ttgcctatgc tcttgccgtg
    13921 ccacttggtt cagccatggc tcgtttcaag aacacttgga ttgatagcct ctcaacaggg
    13981 gctttgacct tcttgcttgc tcttccaacg attgccttgg tttacatcgt tcgattgatt
    14041 ggatcatcta tttcccttcc agattcattc cctatcttgg gagctggaga ttggcgttct
    14101 tacgttttac cagcagtcat ccttggtttg ttgggtgctc ctggtacagc catttggatt
    14161 cgtcgttaca tgattgactt gcaatctcaa gactttgttc gtttcgctcg tgcaaaaggt
    14221 ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa atgccatggt tccgctggtt
    14281 tcaggaattc ctggtgccat tattggggtt atcggtggtg caacccttac tgaaacagtc
    14341 ttcgccttcc caggtatggg taaaatgttg attgactctg taaaagcatc taataactct
    14401 atggtcgttg gtcttgtctt catctttaca tgtatttcta tcttctcacg tcttttggga
    14461 gatatttgga tgactattat tgacccacgt attaaattga ctgagaaagg aggcaaataa
    14521 tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg tgacgatttt gcctctgaaa
    14581 ctattgatgc gccagcatat tcttactgga aatcagtgtt taaacaattt atgaagaaaa
    14641 aatcaactgt agtcatgttg ggaatcttgg tagccatcat tttgataagt ttcatctacc
    14701 caatgttttc taagtttgat ttcaatgatg tcagcaaggt aaacgacttt agtgttcgtt
    14761 atatcaagcc aaatgcggag cattggttcg gtactgacag taacggtaaa tcgctctttg
    14821 acggtgtctg gttcggagct cgtaactcca tcctcatttc tgtgattgcg acagtgatta
    14881 acttggttat cggtgttttt gtcggtggta tttggggtat ttcaaaatca gttgaccgtg
    14941 tcatgatgga agtttacaac gtcatctcaa acatcccacc tcttttgatt gttattgtct
    15001 tgacttactc aatcggagct ggattctgga atctgatttt tgccatgagc gtaacaacat
    15061 ggattggtat tgccttcatg atccgtgtgc aaatcttgcg ctatcgtgac ttggaataca
    15121 acttggcgtc acgtactttg ggaacaccaa ccttgaagat tgttgccaaa aatatcatgc
    15181 ctcaattggt atctgttatt gtgacaacca tgactcaaat gcttccaagc tttatctcat
    15241 acgaagcctt cttgtctttc ttcggtcttg gattaccgat tacagtgcca agtttgggtc
    15301 gtttgatttc ggattattca caaaacgtaa caaccaatgc ttacttgttc tggattccat
    15361 tgacaaccct tgtcttggta tccttgtccc ttttcgtagt tggtcaaaac ttagcggatg
    15421 ctagtgatcc acgtacacat agataggagt agaaatgaca aaagaaaaaa atgtaatttt
    15481 gactgctcgc gatattgtcg tggaatttga cgttcgtgac aaagtattga cagccattcg
    15541 cggcgtttcc cttgaactag tcgaaggaga agtattagcc ttggtaggtg agtcaggatc
    15601 aggtaaatct gttttgacaa agaccttcac aggtatgctc gaagaaaatg gtcgcattgc
    15661 ccaaggtagt attgactacc gtggtcagga cttgacagct ttatcttctc acaaggattg
    15721 ggaacaaatt cgtggtgcta agattgcgac tatcttccag gacccaatga ctagtttgga
    15781 ccccattaaa acaattggta gtcagattac agaagttatt gtaaaacacc aaggaaaaac
    15841 agctaaagaa gcgaaagaat tggccattga ctacatgaat aaggttggca ttccagacgc
    15901 agatagacgt tttaatgaat acccattcca atattctgga ggaatgcgtc aacgtatcgt
    15961 tattgcgatt gcccttgcct gccgacctga tgtcttgatc tgtgatgagc caacaactgc
    16021 cttggatgta actattcaag ctcagattat tgatttgcta aaatctttac aaaacgagta
    16081 tcatttcaca acaatcttta ttacccacga ccttggtgtg gtggcaagta ttgcggataa
    16141 ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga acggttgagg aagtcttcta
    16201 tgaccctcgc catccatata catggagtct cttgtctagc ttgcctcagc ttgctgatga
    16261 taaaggggat ctttactcaa tcccaggaac acctccgtca ctttatactg acctgaaagg
    16321 ggatgctttt gctttgcgtt ctgactacgc aatgcagatt gacttcgaac aaaaagctcc
    16381 tcaattctca gtatcaaaga cacattgggc taaaacttgg cttcttcatg aggatgctcc
    16441 aaaagtagaa aaaccagctg tgattgcaaa tctccatgat aagatccgtg aaaaaatggg
    16501 atttgcccat ctggctgact aggaggaagg aaatgtctga aaaattagta gaaatcaaag
    16561 atttagaaat ttccttcggt gaaggaagta agaagtttgt cgcggttaaa aatgctaact
    16621 tctttatcaa caagggagaa actttctcgc ttgtaggtga gtccggtagt gggaaaacaa
    16681 ctattggtcg tgctatcatc ggtctaaatg atacaagtaa tggagatatc atttttgatg
    16741 gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc ggaattgatt cgtcgaatcc
    16801 agatgatttt ccaagaccct gccgcaagtt tgaatgaacg tgcgactgtt gattatatta
    16861 tttctgaagg tctttacaat caccgtttat ttaaggatga agaagaacgt aaagagaaag
    16921 ttcaaaatat tatccgtgaa gtaggtcttc ttgctgagca cttgactcgt taccctcatg
    16981 aattctcagg cggtcaacgt caacgtatcg gtattgcccg tgccttggtc atgcaaccag
    17041 actttgttat tgcagatgag ccaatttcag ccttggacgt ttctgtacgt gcccaagtct
    17101 tgaacttgct caaaaaattc caaaaagagc tcggcctgac ctatctcttc atcgcccatg
    17161 acttgtcggt tgttcgcttt atttcagatc gtatcgcagt tatttacaag ggtgttattg
    17221 tagaggttgc agaaacagaa gaattgttta acaatccaat tcacccatat actcaagcct
    17281 tgctttcagc ggtaccaatc ccagatccaa tcttggaacg taagaaggtc ttgaaggttt
    17341 acgacccaag tcaacacgac tatgagactg ataagccatc tatggtagaa atccgtccag
    17401 gtcactatgt ttgggcgaac caagccgaat tggcacgtta tcaaaaagga ctaaactagt
    17461 aatggtttta taatttccat gtcaattttt atggaaatta taaaactttt tttgttggac
    17521 tgatttgaaa aactttgaaa gcttcctgaa agaatttttg aaaaatttta aaaattatga
    17581 aatgttattg acaggtgatg aaaaaggtgg taggacagga agcatcaaaa aagaaagcag
    17641 aaaaagaaat gaataagaga caagccgtaa ctatgaatca aaacttttac tttagctact
    17701 ttagatagtg tttgtagaca tgtcaccatg gatgcaaaca gttcaagcaa tttgtttgta
    17761 tctatgtggc taagattttt gattgtggtc catatagatg aatatctaaa tggactagct
    17821 aagttgtaac ttgttaggtt atttcaagta tccgtttaga tagtatctag gcggtttttt
    17881 gttttatctt ttctgagaag gagtttcatt atgaaagttg ttcgaaaatc actagccccc
    17941 tcttggtagt ggggctcctc ttgacttctc tgatcagttt gcatcagttg aaggcagata
    18001 agaaaaagat gtctttcgta tcggtatttc gcaatttatt acccaccagt ccttatacgc
    18061 tactagagaa ggatttgtgg atgagctggc caaacaaggc tatgttgagg gggaaaatat
    18121 tgagattgat ttgcaaaatg cacagggaga acaaagaaat ctaaaaacga tttcccagca
    18181 actagcagaa tccagcgatg tcgttctagc catcgcaagg ccttctgctc agagcttggc
    18241 caatacgacg caaacgacac cagttatctt ttcagctgta acagacccag tcagtgccaa
    18301 gttggttgag tcaagagaac atcctggggg caatgtaact gggacaagcg atcagtcatc
    18361 agatgccatt tcaacccaaa tcaatttgat aaagaaagtg ttgccaaagg ctaaaacgat
    18421 tggaatcctc tatactcaga gcgagcccaa ttcggttgtt caaaaggatg aagctaagcg
    18481 acttctgaaa gaaaaaggct ttaccgttgt tgaaaaaaca atcttggaca gtaataacgt
    18541 caaggcggca gcagagagtt tgatggcaga ggtggatatg gtttttgtcc caactgataa
    18601 tatcatttcg ttaaccatgg aaacagttaa gcaggtttct attaaacaca aggttccagt
    18661 attcggtggt tcaacagaaa tgattgctgt tggtggcttg tataactacg gaaccaatta
    18721 tgaagaattg gtacgtcaga cagctcgcat gctgattcgt gttttaaaag gtgagaaacc
    18781 agaaaatata gcagttgagt tgcctgaaaa actggaattg cataccaatc aagaaatggc
    18841 agatgcattg ggaattgata ttagtaagtt agaaggcaag gaataaggag gattaagatg
    18901 aattttgtgt tatctagttt atcagaaggt ttgttgtggt cgattgtggc gattggggtc
    18961 tacttgactt tccgtatttt ggatattgcg gatatgactg ctgagggagc ctttcctctg
    19021 ggggcagcag tcgttgtatc acagatacag gcaggggcaa atccttggct tgcgacttta
    19081 cttgctttgc tggcaggtat ggtagcaggt cttgtatcag gaatgctcca caccaagatg
    19141 aaaatcccag ctctcttgac agggattgtg accttgacag ggctctattc aatcaatatt
    19201 aaaatcatgg gcagtgtacc caatctttcc ttgggagatt ctgcaaccgt ctttaaacag
    19261 ttggcgagct tgggtctgac aaatgaagga actgttttct cacttagttt ggcctgtttt
    19321 ttacttgttt gtttggtctt gacccttttg atgaaaacag agattggcct ggtcttgcgt
    19381 tcgactgggg ataatatccc gatgagtgag gccaatggag tcaatgtaga caccatgaag
    19441 attgttggtt acatgatttc aaacggtttg attgctctat gtggttcctt gtttgcccaa
    19501 aatgatggat tttcagatgt gacttctggg acaggaacca ttgttgttgg tttgagctcg
    19561 gtgattattg cggaagtctt gatacacgac ttgaccattg gaggccgctt attatccatc
    19621 ggaatcggtg ctattgttta ccgtttgatt attttaaata tctatgaaat tccaaatcta
    19681 gatcaaaact tggtacgtct ctttaatgca atcttgctcg ctttggtttt atttgcacca
    19741 gaattgcaaa agagattaaa gattcgtggt ctgaaattga gaaatgaata ggaggagaaa
    19801 gttatgacaa gtttgttaac acttgaaaat attcacaaga catttgaagc agggacggtc
    19861 aatgagaacc atgtcctcaa aggattagat ttagaggttg aagagggaga ttttatctct
    19921 gtgattggtg gaaatggagc aggaaaatcc actttgatga atatcttggc tggcaatcta
    19981 tcggtggacg aaggggacct tctactggca gggaaatcca ttaaaaatct gagtgtcaga
    20041 aagagagcca aggatattgc ccgtgttttt caagatccta agatgggaac agcttctcgt
    20101 ttgacgattg aggagaatat ggctattgcc ttgagacgcg ggcagaagag agggcttggt
    20161 tggggcgtga aggagaagga tagaatccag ttccaagagg ccttgaagga gttgaatatt
    20221 ggtcttgaaa accgcttaaa agtagatact caatatctct caggaggaca aagacaggct
    20281 ttgactctag tcatgacaga tctgatgaaa ccaaaacttt tacttttgga tgaacatact
    20341 gcggcacttg acccgaaaac cagtcaaatg gtgatggact tgacgcaaaa gattgtggag
    20401 caccatcagt ggacgacttt gatgattacc catgatatga atcatgcgat tgagtacggc
    20461 aatcgtctca ttatgctcta tcaaggcaag atagtggtgg atgtcaaggg agaagagaaa
    20521 aagcatctga cagttgaaga ccttatgcat ctcttccaga aaaatagtgg ccaaagtctg
    20581 gtcagtgatg aattagtttt gggataaaga aaaaggctga gatctggtgt ctcagtcttt
    20641 tatttcttgc gttttgcaaa atctacaaat ctaaatttct ctaacttatg gcgactttcg
    20701 gtaaactgaa attggcgtcc gtcttggaga tagacttttg atttaatcga aacgacataa
    20761 gggtctttgc caatgtccat gagaatcttg tctcggtcgc ttgagtggtc aatggtgatt
    20821 tccttctgag cataatcaat aaggagtttg aggccatttt ctatataaga atagatagac
    20881 tgctcagcaa tttggcgagt gagatttgga attagttcca tatccagata gtccgtatcc
    20941 agaacggata ccagatcatc caccacacgc tggcggacca ccttccaaac catccgaaac
    21001 tctgggaaac cggttatcag tgaggatttt ttatcaataa taatcttgtc cagactgacc
    21061 acgttggttt tagagcgcag tccaagttct ttaactagtt cttggtagct ggttaggttg
    21121 gatacaggga aattgacggt ttcttctttg acgacttgag aaccttgctc tcttatcttt
    21181 ttgatcaatc cttcctcttg gaggagagac agggcctttc ggacggtatc acgactcact
    21241 tgatattgct ccataaggtc gtgctcgcta ggaaggaaat ctccgacagc gtaagtctcg
    21301 ttttgaatgg tttcttggat ttgcttaaat aattgttggt atttcttcat tttgcctgct
    21361 ttaataggtt tttagtctag tttaatgaac acctgtatct attttaccat acaagtgcta
    21421 gaaaatttac aaaaaaacac cctttttata ccaaacaagt tgcagacaag tttggtatcg
    21481 tttacaatat acttatcaaa tcaaacttgc tttgacaagt ataaggagaa tcaaatggga
    21541 aaatttgaac aagaagccaa agatctgctt caggcaattg gaggcaaaga aaatgtgact
    21601 gccgtaactc actgtgcgac acggatgcgc tttgttttag gagatgataa gaaggctaat
    21661 gttaaagcta tcgagtcaat tccagctgtt aaaggaactt ttacaaatgc aggtcaattt
    21721 caggtaatca ttggaaatga cgtgcccatc ttttataatg attttacagc cgtttcaggt
    21781 attgagggtg tttccaaaga agcagccaag tctgcagcta agagtaatca aaacgtggtc
    21841 caacgtgtta tgaccactct ggcggagatt tttactccga ttattccagc cttgatagtc
    21901 ggaggattga tcctcggttt ccgtaatgtc ttggaaggtg tccattggtc gatgttggat
    21961 ggcaagacca tcacagaatc ctctcagttt tgggcaggtg tcaatcactt cctctggttg
    22021 cctggtgaag ctatcttcca gttcttacca gtagggatta cttggtctgt ttctcgtaag
    22081 atgggaacca gccaaatttt gggaattgtt ctcggaatct gtttggtatc gcctcagttg
    22141 ctcaatgcct atgcggttgc ttcaacgcca gcagctgata tcgcggcaaa ctgggtttgg
    22201 aattttggct attttactgt taatcgtatc ggttaccaag cccaagttat cccagccttg
    22261 cttgcaggtt tgagtctgtc ttatcttgaa atcttctggc gcaagcatat cccagaagtc
    22321 atttctatga tttttgtacc tttcttgtca ttgattccag ccttgatttt ggctcatact
    22381 gttttgggac caatcggttg gacaattgga caaggacttt catcagttgt cttggcaggt
    22441 ttaactggtc cagttaaagg gctcttcggt gcaatttttg gtgccctcta cgctccattt
    22501 gtcatcacag gtctgcacca tatgaccaat gccattgata cacaattgat tgcggatgct
    22561 ggtggcactg ccctatggcc aatgattgct ctttctaata ttgctcaagg ctcagccgtg
    22621 tttgcctatt atttcatgca tcgccatgat gagcgtgagg ctcaggtttc acttcctgca
    22681 accatttcag cctatctcgg tgttacagaa ccagctcttt ttggggttaa cgtaaaatat
    22741 atttatccat ttgttgctgg gatgactggt tcagcccttg caggcatgtt atccgttact
    22801 tttaatgtaa ctgcggcttc tattggtatc ggtggtttgc caggtattct ctctattcaa
    22861 cctcaataca tgctgccatt tgcaggaact atgctagttg cgattgttgt tccaatgctc
    22921 ttgactttct tcttccgcaa ggctggtctc tttacaaaaa cagagggcga tacgaacttg
    22981 caggcagaat tcgttgctca agaagaagca gaatttgtga accatgaacc agtagaactt
    23041 acttcggtag aaattatcag cccactaact ggccaagtga aagaattgag tcaagcgacg
    23101 gatcctattt ttgcatcagg tgtcatgggg caaggtctgg tcattgaacc aagccaaggt
    23161 gagttgacct ctccagttaa tgggacagtg acggttcttt tccctaccaa gcatgccatc
    23221 ggcattgtct ctgacgaggg agttgaattg ctcatccaca tcggtatgga tacagtaggt
    23281 cttgatggca aaggttttga aagtcttgta gtccaaggag atcacgttac agttggtcag
    23341 caactgattc gttttgatat ggatgtcatt aaggctgcag gtctggtgac agaaactcct
    23401 gttatcatca ccaaccaaga tgcttataca gcgactattc ccggaactta tccgacaacg
    23461 attcaagctg gagcatctct catggtcgct acacgaatct aatactcttc gaaaatctct
    23521 tcaaaccacg tcagctctat ctgcaacctc aaaacagtgt tttgagcaac ctgtggctag
    23581 cttcctagtt tgctctttga ttttcattga gtataattga aatagcacaa atagggacgg
    23641 aagactgctt cctccctttt atcagaaaag gagaaacaga tgacacttga taaaggaaaa
    23701 gtagtctatc aaatctatcc aaaatcttac aaagacacca ctgaaaacgg tattggggat
    23761 ttccgtggga ttatcgaaaa aattccctat ttagccaaac taggcgtgga tatggtctgg
    23821 ctcaatccat tctatccaag ccctcaacgg gataatggtt acgatatttc agattatatg
    23881 gcagtggatc ctctttttgg tgatatggct gattttgagg aaatggtgtg tgtcggtaaa
    23941 gagcacaaga ttgactttat gctggacatg gtacttaatc attgttcgac agagcatgaa
    24001 tggtttcaga aagccctagc tggtgataag tattatcaag actttttctt catccaagac
    24061 caaccaacag attggcagtc taagtttggt ggttctgcat gggcgccttt cggggatact
    24121 gggaaatact atcttcacct atttgatgag acccaggctg accttaactg gcgcaattcc
    24181 aatgtccgta aggagctttt caaggttgtt aatttctggc gcgacaaggg tgtcaaaggt
    24241 ttccgatttg atgtgatcaa tttgatcggt aaggacgagg tttcagtgga ctgtcctgaa
    24301 aatgaaggga agccagctta tacagacaaa cccatcgttc ataactattt gcgtatgatg
    24361 aaccaagcca cttttggatc tgacgatagc tttatgacag ttggggaaat gtcttctacc
    24421 accatggaaa actgtgtcct ctattcgtcg cctgatcgtc aggaattatc catgaccttt
    24481 aattttcatc acctcaaggt ggactataag gatggacaaa agtggacctt ggctcccttt
    24541 gattttgaag agttgaagag tctttaccat agttggggta aggaaatgag tgataaagac
    24601 ggttggagtg ccctcttttg gaacaatcac gatcaaccac gtgccttgaa ccgttttgtc
    24661 gatattcaaa acttccgcaa ggaaggagcc accatgctag cagccagcat tcacctgtca
    24721 cgtgggacac cttatatcta catgggtgag gaaatcggga tgattgaccc agactatgat
    24781 tctatggctg attatgtgga tgtcgaatcg cttaatgcct atcagatgct cttagaagaa
    24841 ggaaagagtc agcaagaagc cttccagatt attcaagcta agtcgcgtga taattcacgt
    24901 attcccatgc agtgggatac ttctgaaaat gcaggatttt caacaggcac tccatggttg
    24961 aaagtcggta aatcctataa agatatcaat gtagaaaatg aaatcaaagg tctgattttc
    25021 accttctatc aagacttgat tcgactgcgt aaggaaatgc caatcatttc agaaggaaat
    25081 tacaaaccag cctttgaaga tagcaaacaa gtctacgctt ttgaacgcca gtttgaggat
    25141 caaaagttac tagtgctcaa taacttttat gccaaagaag tggaaatcga cttgccagca
    25201 gtctaccaaa atggacagat tttgatttca aactatgaag atgcagaagt gtctgagaaa
    25261 attctactca aaccctatca aacactggct atttatgtaa attaatactc ttcgaaaatc
    25321 tcttcaaacc gtgtcagttt tatctgtaac ctcaaagcgg tgctttgagc aacctgtggc
    25381 tagcttccta gtttgctctt tgattttcat tgagtataaa cttagcaagg agacctcttt
    25441 ttcagaaaag cagggttctt tttgctgtct attccatcct tgaaagtgta gaacaaactg
    25501 gtcagggtgg ctagtttgtg gtataatgaa agggattcaa aaagaaacag gagaaaaatg
    25561 atggatttac ttttagcaat tgtattgatt gtgctagctt ttctaggagg agctcttgga
    25621 ggaatgtact tggttcgtaa gcaaatcgaa aaagaatttg ctgacaaccc acgtttgaat
    25681 gctgaagcag ttcgtactct tttgagtgca aatggtcaaa aaccaagcga agctaaggta
    25741 caacaagttt accaccaaat catccgccaa caaaaggcag cccttgctaa taataaaaag
    25801 aaaaaataaa taaggaaaag tctgggatga aagttccaga cttctcacta tgttggttat
    25861 ggtttaggga tgagactttt tccttgcatt ctattgatat ttgattatga ttaagagaga
    25921 ggtagttgac attctatcaa gctgtcggtc agttcgttca gtacaaggaa tcataatgaa
    25981 cgatcaatca gaaaaaagac tagaaagaag actgtatgga taatcgacca attggttttt
    26041 tggattcggg tgtcgggggc ttgaccgttg tgcgcgagct catgcgccag cttccccatg
    26101 aagaaatcgt ctatattgga gattcggcgc gggcgcccta tggcccccgt cctgctgagc
    26161 aaattcgtga atatacttgg cagctggtca actttctctt gaccaaggat gtcaaaatga
    26221 ttgtcattgc ttgtaacact gcgactgcgg tcgtctggga agaaatcaag gctcaactag
    26281 atattcctgt cttgggtgta attttgccag gagcttcggc agccatcaag tccagtcaag
    26341 gtgggaaaat cggagtgatt ggaacgccca tgacggtaca atcagacata taccgtcaga
    26401 aaatccatga tctggatccc gacttacagg tggagagctt ggcctgtccc aagtttgctc
    26461 ccttggttga gtcaggtgcc ctgtcaacca gtgttaccaa gaaggtggtc tatgaaaccc
    26521 tgcgtccctt ggttggaaag gtggatagcc tgattttggg ctgtactcat tatccactcc
    26581 ttcgccctat tatccaaaat gtgatggggc caaaggttca gctcatcgat agtggggcag
    26641 agtgcgtacg ggatatctca gtcttactca attattttga aatcaatcgt ggtcgcgatg
    26701 ctggaccact ccatcaccgt ttttacacaa cagccagtag ccaaagtttt gcacaaattg
    26761 gtgaagaatg gctggaaaaa gagattcatg tggagcatgt agaattatga caaataaaat
    26821 ttatgaatat aaggatgacc aggactggta tgttgggtct tatagtattt ttggtggcgt
    26881 taacagtttg agcgactata agacagattt tcctctgttt gaattctcca aaatatttgg
    26941 agatgaagag tatggtttcc cgctttcagt tactgtttta cgctatggtt ctacctaccg
    27001 tttgttctcc tttgtggtag acatgcttaa tcaagaaatg ggacgaaact tggaagttat
    27061 tcaacgtcat ggggccctgc tcttggttga aaatgggcaa ctcttgtatg tagaattgcc
    27121 taaagaaggg gtcaatgttc atgatttctt tgagacaagc aaggtcagag aaaccttgtt
    27181 gattgcgact cgtaacgaag gtaaaaccaa ggaattccga gctatctttg ataagttagg
    27241 ctacgatgtg gaaaatctta atgactaccc tgacctgcct gaagtagcag aaacaggtat
    27301 gacctttgaa gaaaatgccc gccttaaggc agaaaccatt tctcaattaa cgggcaagat
    27361 ggttttggca gatgattctg gtctcaaagt cgatgtcctt ggtggcttac caggcgtctg
    27421 gtcagctcgt ttcgcaggtg tgggagcaac tgaccgtgaa aataatgcca aactcttgca
    27481 cgaattggcc atggtctttg aactcaagga ccgctcggct cagttccaca caaccctagt
    27541 cgtagccagc ccaaataagg aaagtttagt tgttgaagca gactggtcag gttatattaa
    27601 ctttgaacct aagggtgaaa atggctttgg ctatgatccc ctcttccttg taggagagac
    27661 aggtgagtca tcagctgaat taaccctaga agaaaaaaat agtcaatctc accgtgcctt
    27721 agccgttaag aaacttttgg aggtatttcc atcatggcaa agcaaaccat cattgtaatg
    27781 agcgattccc atggcgatag cttgattgtg gaagaagtcc gtgatcgcta tgtgggcaaa
    27841 gtcgatgccg tttttcataa cggcgattct gaactacgtc cggattctcc actttgggag
    27901 ggcatccgcg ttgttaaagg gaacatggac ttctacgccg gctacccaga acgtctggtg
    27961 actgagcttg gttcgaccaa gattatccaa actcatggtc acttgtttga catcaatttc
    28021 aactttcaaa agttggacta ctgggctcag gaggaagagg ccgctatctg cctctatggt
    28081 cacttgcatg tgccaagtgc ttggttggaa ggcaagatcc tctttctaaa tccaggttct
    28141 atcagtcaac cacgaggtac catcagagaa tgtctctatg ctcgtgtgga gattgatgat
    28201 agttacttca aagtggactt tttgacacga gatcacgagg tgtatccagg tttgtccaag
    28261 gagtttagcc gatgattgcc aaggagtttg agactttctt gttggggcag gaggaaactt
    28321 ttttgacccc tgctaaaaat ctagctgtgt tgattgatac ccacaatgcg gatcatgcga
    28381 ccctcttgct cagtcagatg acctataccc gtgttcccgt tgtgacagat gaaaaacagt
    28441 ttgttgggac gattggactc agagatatta tggcttatca gatggagcat gacttgagcc
    28501 aagaaatcat ggcggatacg gatatcgttc atatgacaaa aacggacgta gcggttgttt
    28561 cgcctgattt caccattacg gaggtcttgc acaagctagt agatgagtcc ttcttaccgg
    28621 tcgtggatgc agagggtatt ttccaaggga ttattacgcg caagtccatc ctcaaggccg
    28681 ttaatgccct cttgcatgac tttagtaagg aatatgagat tcgatgccaa tgagagacag
    28741 gatttcagcc tttttagagg aaaagcaggg cttgtctgtc aattccaagc agtcctataa
    28801 gtatgatttg gagcaatttt tagacatggt aggtgagcgg atttctgaga ccagtctcaa
    28861 gatttaccaa gcccagctag ccaatctaaa aatcagcgcc cagaagcgaa agatttcggc
    28921 ctgtaaccaa tttctatact ttctctatca aaaaggagag gtggacagct tttaccgctt
    28981 ggaattagcc aaacaagctg aaaagaagac ggaaaagcca gagattctat acctagactc
    29041 tttttggcag gaaagcgacc atccagaggg ccgcttgcta gcgctcttaa tcctagaaat
    29101 ggggctcttg cccagtgaga ttttagccat caaggttgcg gacatcaatc tggattttca
    29161 ggtgctgcga atcagcaagg cttcccaaca gaggattgtc accattccca cggccttgct
    29221 ttcagaattg gaacccttga tggggcagac ctatcttttt gaaagaggag agaaacccta
    29281 ttctcgtcag tgggcctttc gtcagttaga atcttttgtc aaggagaaag gttttccatc
    29341 cttatcagct caagtcttac gtgaacagtt tattctaaga caaatagaaa acaaggtcga
    29401 tttgtacgaa attgcaaaaa aattaggatt aaaaacagtc ctgaccttag aaaaatatag
    29461 ataatggata ttaaattaaa agattttgaa ggacccctgg acttgctctt gcatctggtt
    29521 tctaagtacc agatggatat ctacgatgtg cccattacgg aagtcatcga acagtatcta
    29581 gcctatgtct caaccctgca ggccatgcgt ctggaagtga cgggtgagta catggtcatg
    29641 gctagtcagc tcatgctgat taagagtcgt aaactccttc cgaaggtagc agaagtgaca
    29701 gacttggggg atgacctgga gcaggacctc ctctctcaaa tcgaagaata tcgcaagttc
    29761 aagctcttgg gtgagcactt ggaagccaag caccaagaac gggcccagta ttattccaaa
    29821 gcgccgacag agttgattta cgaagatgcg gagcttgtgc atgacaagac gaccattgac
    29881 ctctttttgg ctttttcaaa tatcctagcc aagaaaaaag aggagtttgc acaaaatcac
    29941 acgacgatct tgcgggatga gtataagatt gaggacatga tgattatcgt gaaagagtcc
    30001 ttgattggac gagatcaatt gcgcttgcag gatttgttca aggaagccca gaatgtccaa
    30061 gaggtcatca ccctcttttt ggcaacccta gagttaatca aaacccagga gctgatcctc
    30121 gtgcaagagg agagttttgg agatatctat ctcatggaaa agaaggaaga aagtcaagtg
    30181 cctcaaagct agacttgata gagaggaaag atgagtactt tagcaaaaat agaagcgctc
    30241 ttgtttgtag cgggtgaaga tgggattcgg gtccgccagt tagctgaact cctctctctg
    30301 ccaccgacag gcatccagca aagtttagga aaattagccc agaagtatga aaaggaccca
    30361 gattccagtt tggctttgat tgagacaagt ggtgcttata gattggtgac caagcctcaa
    30421 tttgcagaga ttttgaagga atactctaag gcgcctatca accagagctt gtctcgggct
    30481 gcccttgaga ccttgtccat tattgcctac aaacagccga ttacgcggat agaaattgat
    30541 gccatccgtg gagttaactc gagtggagcc ttggcaaagt tgcaggcttt tgacctgata
    30601 aaggaagacg ggaaaaagga agtattgggg cgccccaacc tctatgtgac tacggattat
    30661 ttcctagatt acatggggat aaaccattta gaagaattac cagtgattga tgagcttgag
    30721 attcaagccc aagaaagcca attatttggt gaaaggatag aagaagatga gaatcaataa
    30781 gtatattgcc cacgcaggtg tggccagtag gagaaaagca gaagagctga ttaagcaagg
    30841 cttggtgacg gttaacggcc aagtggtgcg tgaactagca accactatca agtcaggcga
    30901 caaggtcgaa gttgaaggtc aacctatcta caacgaagaa aaggtctact atctgcttaa
    30961 caaaccacgc ggtgtgattt ccagtgtgac agatgataag ggtcgcaaga cggttgtcga
    31021 cctcttgccc aatgtcaaag agcgtattta ccctgtgggt cgtttggact gggatacatc
    31081 aggtgtcttg attttgacca atgatgggga ctttacagac gagatgattc accctcgtaa
    31141 tgagattgac aaggtttatg tcgcgcgtgt taaaggtgtg gccaataagg acaatctccg
    31201 ccccttgacc cgtggtcttg agattgatgg taagaaaacc aagccagctg tttatgaaat
    31261 tctcaaagtg gacccagtca aaaatcgctc tgtggtgcag ttgaccatcc atgaagggcg
    31321 taaccatcag gttaaaaaga tgtttgaagc tgttggtctc caagtagata agttgtctcg
    31381 gactcgtttc ggacacctag acttgacagg actccgtcca ggagaatccc gtcgtcttaa
    31441 taaaaaagaa atcagccaac tacacaccat ggctgtaact aagaaataat gaaacgaatt
    31501 ttaatagcgc ctgtgcgctt ttaccaacgt tttatctcac cagtctttcc accctcttgt
    31561 cgctttgagc tgacttgttc caactacatg attcaggcta ttgaaaaaca tggttttaag
    31621 ggggtattga tgggcttggc tcggatttta cgttgtcatc cctggtcgaa aacaggtaag
    31681 gaccccgttc cagaccactt ttcccttaaa cgaaatcaag aaggggaatg aggtggggta
    31741 aatagatttc aaaatgataa aaacgcatcc tatcaggttt gagtaaactt gataggatgc
    31801 gttttagaat gtcaaaattt tatactcttc gaaaatctct tcaaaccgcg tcagctttca
    31861 t
//
LOCUS       DBEJAQ010000026        29384 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.026, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000026 DBEJAQ010000000
VERSION     DBEJAQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 29384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.026"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..1099
                     /gene="phtD"
                     /locus_tag="ACL297_001216"
     CDS             <1..1099
                     /gene="phtD"
                     /locus_tag="ACL297_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HGR0071482.1"
                     /translation="LKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQV
                     AKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKE
                     KGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPH
                     YDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQR
                     NKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDSSIRQNAVETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESK"
     gene            1516..1896
                     /gene="dhaM"
                     /locus_tag="ACL297_001217"
                     /pseudo
     CDS             1516..1896
                     /gene="dhaM"
                     /locus_tag="ACL297_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
     gene            2354..3067
                     /locus_tag="ACL297_001218"
     CDS             2354..3067
                     /locus_tag="ACL297_001218"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGR0071483.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            3125..3529
                     /locus_tag="ACL297_001219"
     CDS             3125..3529
                     /locus_tag="ACL297_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071484.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQ"
     gene            3865..4518
                     /locus_tag="ACL297_001220"
     CDS             3865..4518
                     /locus_tag="ACL297_001220"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGR0071485.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            4528..4992
                     /locus_tag="ACL297_001221"
     CDS             4528..4992
                     /locus_tag="ACL297_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGR0071486.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            5005..6273
                     /locus_tag="ACL297_001222"
     CDS             5005..6273
                     /locus_tag="ACL297_001222"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGR0071487.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKFNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            6287..6547
                     /locus_tag="ACL297_001223"
     CDS             6287..6547
                     /locus_tag="ACL297_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071488.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            6558..7919
                     /gene="pdrM"
                     /locus_tag="ACL297_001224"
     CDS             6558..7919
                     /gene="pdrM"
                     /locus_tag="ACL297_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HGR0071489.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKY"
     gene            8073..9041
                     /locus_tag="ACL297_001225"
     CDS             8073..9041
                     /locus_tag="ACL297_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGR0071490.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            9057..10049
                     /locus_tag="ACL297_001226"
     CDS             9057..10049
                     /locus_tag="ACL297_001226"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGR0071491.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNSVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            10267..11310
                     /locus_tag="ACL297_001227"
     CDS             10267..11310
                     /locus_tag="ACL297_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HGR0071492.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            11356..13059
                     /gene="lpdA"
                     /locus_tag="ACL297_001228"
     CDS             11356..13059
                     /gene="lpdA"
                     /locus_tag="ACL297_001228"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGR0071493.1"
                     /translation="MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKV
                     SMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTS
                     NDDDKSDDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTY
                     LHNAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTV
                     HKGIGTITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNE
                     VPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGM
                     TILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCI
                     KVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIY
                     TLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGV
                     HIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
                     K"
     gene            13123..14112
                     /locus_tag="ACL297_001229"
     CDS             13123..14112
                     /locus_tag="ACL297_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGR0071494.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(14185..15255)
                     /gene="xerS"
                     /locus_tag="ACL297_001230"
     CDS             complement(14185..15255)
                     /gene="xerS"
                     /locus_tag="ACL297_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HGR0071495.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(15839..17389)
                     /locus_tag="ACL297_001231"
     CDS             complement(15839..17389)
                     /locus_tag="ACL297_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGR0071496.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(17405..18184)
                     /locus_tag="ACL297_001232"
     CDS             complement(17405..18184)
                     /locus_tag="ACL297_001232"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGR0071497.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(18171..19022)
                     /gene="ylqF"
                     /locus_tag="ACL297_001233"
     CDS             complement(18171..19022)
                     /gene="ylqF"
                     /locus_tag="ACL297_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGR0071498.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(19276..24489)
                     /gene="zmpD"
                     /locus_tag="ACL297_001234"
     CDS             complement(19276..24489)
                     /gene="zmpD"
                     /locus_tag="ACL297_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease D"
                     /protein_id="HGR0071499.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            complement(24533..>29384)
                     /locus_tag="ACL297_001235"
     CDS             complement(24533..>29384)
                     /locus_tag="ACL297_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGR0071500.1"
                     /translation="IVEPEKVEAPKEYTGVQAGAIVEPEKVEPSKEYTGVQAGAIVEP
                     EQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPSAEDTKPNNENTNTPEEMSIQK
                     KSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHNFINNSTDNTTGEFRGDKSKNV
                     YEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVIKLPETTGREHVELVKNYSKVN
                     LNDKLNVHGYPARKYTHLKDATVEMEQEYANNTYGVQYQGGNPGMSGGGIFNANGEVI
                     GVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVYDELYRHKDEKKDDAKDEKEVI
                     KKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENYFMKVKSENFKDVMLPVTSITN
                     DTKDNRDVYKIVASANSLIQHENNNVLENYTYYLPKTQQSETGVYTSFKNLVDAMNSN
                     PNGTFRLGATMDAREVELPDGQESYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNG
                     ATVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHADGAIAGERSIGGLVSQVNNST
                     ISNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGG
                     IVGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNV
                     TNGNAIAGYNFNGIKANGTYSNKNNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIKEK
                     KATFVSKNTIKTEDFNFSSRYVTDYKNLENADSSKEKVYKNIEKLLPFYNRETIVKYG
                     NLVETSSNLYNKELLSVVPMKDKEVISDINKNKSSINKLLLYYADNTSETLNVNYQTD
                     FSNVAEYRIGGTNLIYTPNTLLRNYQNILDEVLPALNSVEYKSEAIRKVLDVSKDVSL
                     TELYLEEQFNTTKTNLKDSLTKLLTADAAIAENNNKVIDNYVIEKIKNNKEALLLGLT
                     YLERWYDFKYRDTKAKDLVMYHLDFFGKSNSSALDNVIELGKSGYNNLLAKNNVITYN
                     VLLAKNYKTNNLFDALEKYRKAFVPDKTNNEWFKEQTKAYIVEEKSTIKEVSDKQSIA
                     GSPYSIGVYDRLTSPSWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPA
                     GTDLNDYVEKKAKEAAVRFRDHYDYWYRILDDKNKEKLYRSVLVYDAFRFGTDEKEDK
                     DTYQATFETNHPAIKHFFGPAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHE
                     MTHNSDREIYLGGYGRRNGLGPEFYAKGLLQAPDHPNDPTVTINSILKYDQSEESTRL
                     QVADPTQRFGSVDDLNKYMHNMFDVIYMLEILEGKAVAKLDTNQKYDLLRKIENEYKP
                     DPDGNSVYATNVVRRLKPEELTKLTTFNSLIEHDIITRRGYVDEATYKRNGYYTINLF
                     SPIYSALSSKIGTPGDLMGRRIAFELLAAKGYKDGMVPYISNQYEKEAKAQGKVITSY
                     GKQIGLVTDEIVLSKVFNNQYNSWIDFKKDMYKEREDKFGKLNKVSFIDPNGSWARQQ
                     KVTIDNINRLEKMIEDAVKFDAEDEVAKLYPETNSRVLKLKKAIFKAYLDQTGDFRSS
                     IFENKK"
BASE COUNT         8752 a         5562 c         5544 g         9526 t
ORIGIN      
        1 actcaaggat gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc
       61 tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct acactgatga
      121 tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt atatctttga
      181 tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata tgacccatag
      241 ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc aggcttatgc
      301 taaagagaaa ggtttgaccc ctccttcgac agaccatcag gattcaggaa atactgaggc
      361 aaaaggagca gaagctatct acaaccgcgt gaaagcagct aagaaggtgc cacttgatcg
      421 tatgccttac aatcttcaat atactgtaga agtcaaaaac ggtagtttaa tcatacctca
      481 ttatgaccat taccataaca tcaaatttga gtggtttgac gaaggccttt atgaggcacc
      541 taaggggtat actcttgagg atcttttggc gactgtcaag tactatgtcg aacatccaaa
      601 cgaacgtccg cattcagata atggttttgg taacgctagc gaccatgttc aaagaaacaa
      661 aaatggtcaa gctgatacca atcaaacgga aaaaccaaac gaggagaaac ctcagacaga
      721 aaaacctgag gaagataagg aacatgatga agtaagtgag ccaactcacc ctgaatctga
      781 tgaaaaagag aatcacgctg gtttaaatcc ttcagcagat aatctttata aaccaagcac
      841 tgatacggaa gagacagagg aagaagctga agatactaca gatgaggctg aaattcctca
      901 agtagagcat tctgttatta acgctaagat agcagatgcg gaggccttgc tagaaaaagt
      961 aacagattct agtattagac aaaatgctgt ggagacattg actggtctaa aaagtagtct
     1021 tcttctcgga acgaaagata ataacactat ttcagcagaa gtagatagtc tcttggcttt
     1081 attaaaggag agtaagtaaa ggtagcagca ttttctaact cctaaaaaca ggataggaga
     1141 acgggaaaac gaaaaatgag agcagaatgt gagttctagt tctcattttt ttcatgaaaa
     1201 tgtgcaaaat atagtagatt gaaactagaa tagtatacct ctacttctaa aacattgtta
     1261 gaaatcgatt tgactgtcct gttcttattt tactatatct taacagatag tgtaaataaa
     1321 gataaactat ttactggcta attaatcagt taaacactag ttaaggagta atgatgaaaa
     1381 aaagaacaat actattattg atgaccagtc tgttggctct tgtcttagga gcatatggtt
     1441 tcttggacat attgatcctg gatcattctc atcaggatta ctctttactg ctattttaga
     1501 aactggggtg gtttgatgga aagtattggt attggtcttg ttatcgtttc acattccaaa
     1561 cacattgcag aaggtgttgt tgaactgatt agtaaagtag ctaaagatgt tccgattact
     1621 tatgtaggag gaaccgaggg cggaggaatt ggaacgagtt ttgattaagt agatagggtt
     1681 gtttccgaaa atacagcaga tactttactt gccttttttg acctaggttc tgctaaaatg
     1741 aacttaaaaa tggtgactga tttcagtgat aaaagtatca tcatcaacag ggttccaatt
     1801 gtagaaggtg cctataatgc agctgctctt cttcaggctg gtgcagaact gtcagttatt
     1861 caaacacagt tagcggagct tgaaatcaat aaataaggaa ttttactata actcttttta
     1921 taggtaagct attgattatc tcaactataa aatgttaagt aaatgatttc aaaatcagaa
     1981 agggattgct ttatgcagtt cctttttatt ttaacaggag tgaaactata gtgtttctaa
     2041 attgtgaatc aatcaaaact gattgtgatg gggctattct agctttagaa accttcaaaa
     2101 attaaaattt aaggcaatca attacttgga agagtatgaa agcatttagt ttataggaat
     2161 tctaggtcta gaattacaca tatatttatg aatacggggt gctcgatagt tagtattgtt
     2221 ctattctgaa agatttgagc tgtcagttgt atagaaagtg ttcgaatttt tttaagtgat
     2281 taaattagtt aattgtatga ggtgctttat gatataatgt tcttaatgaa ttttcagaaa
     2341 ggaaaacctc aaattgttct acaaatttct actcttcgac ctcgaccaca ctcttcttga
     2401 ttttgatgct gctgaggatg tggctttgac ccaacttcta aaagaagaag gagttgcgga
     2461 tattcaggct tataaagatt attacgttcc tatgaacaag gctctctgga aagacttgga
     2521 gctgaagaaa atcagtaaac aagagctggt taacacgcgc ttttctcgtt tattttctca
     2581 ttttggacag gaaaaagacg gtagttttct tgcccagcgt taccaatttt atctcgctca
     2641 gcagggacaa acactatcgg gcgctcatga tctcttggac agcctcattg agcgtgatta
     2701 tgacttgtat gctgcgacaa atggcattac tgccattcag acaggacgtt tggctcaatc
     2761 tggtctagca ccttatttca atcaagtctt tatctcagaa cagttgcaaa ctcaaaagcc
     2821 ggatgctctt ttttatgaaa agattggcca gcaaattgct ggatttagta aagaaaagac
     2881 gctgatgatt ggagattctc taaccgccga cattcaaggt ggcaataatg cggggattga
     2941 cactatctgg tataatcctc atcacctcga aaatcacaca caagcccagc cgacttacga
     3001 agtctattct taccaagact tgctggattg tttagataaa aatattcttg aaaagatcac
     3061 attttaaagg agacgagcta atgactacaa aaaagctaat attactattg aagagtacat
     3121 tgaaatgtct gaagttgatt ttaatgaagc tgttaattat gaatttacat ctgacacttg
     3181 tcaattagca aatagtattt atcaatctct ttttaagttt tttgataaga aaaatttctc
     3241 tggcgattta atttttactt ggaaatctcc atcattagtc aaagaagggg attatattgg
     3301 gagaagggat tcacaagtag ataatcttag agtaatagga aatatatttc cgaattatct
     3361 tactaatcga aaatatagcc tcaatatgaa tcgtaatggc tgtatgggag attttcctca
     3421 tgactttttt gatatatacc tagatcatgt agcaaaatat gcctacgaac aaaaagttaa
     3481 taatattaaa gagtattatc ctttaaaaag agcgatttta caccaataga atgcattgta
     3541 ttttcgattt ttttctaatt ttgacgactt tttagaaaaa aattatttaa agactatatg
     3601 gcaagtttct aaagaaactc ctttttctga aatggatttt aatatgttta aaaatatttc
     3661 agaaaagata atcttcgaaa gaggtagtaa aatgcttaat gatttaaaaa gtaattataa
     3721 aaaatagcca gtctttataa actgtattca cgtattcaaa tcgtatatat agtcgattta
     3781 ttaaatcttt agataaactg taaaaagtgg tttccatagc cactttttgc tataatagag
     3841 gcagtaaaaa tgaaggagtc ggctatggaa cactcgtctt ggcatgcttt gattaaggcg
     3901 caattacctg agggttattt cgggaaaatc aatcagttta tggagcaggt ctattctcag
     3961 gggattattt atccacccaa ggaaaaggtt tttcaggctc tcttgacaac actgcttgaa
     4021 gaagttaagg tggtaattct agggcaagac ccctatcacg gaccaggtca agcgcagggc
     4081 ttgagttttt ctgtacctga ctctatccca gctccaccat ccttgcaaaa tatcttgaaa
     4141 gaattgtcag atgatatcgg agttaagaaa tctcatgatt tgacagcttg ggctgagcaa
     4201 ggagtcttgc ttcttaatgc ttgtttgact gttcctgctg gacaggccaa tggtcatgct
     4261 ggtcaaatct gggagccttt tactgatgct gtgattcagg tggtcaatca tctagataga
     4321 ccagtcgttt ttgtactctg gggagcttat gcacgtaaga agaaggcatt agttaccaat
     4381 cctcatcact tgattatcga atcagcccat ccaagtcctt tgtcggttta tagaggattt
     4441 tggggttcca agcccttttc caaggccaat gcattcttaa aagagacagg acaagagcca
     4501 atcgattggc ttagataagg agaaaatatg cctcagttag cgacgatttg ctacattgat
     4561 aatgggaaag aactgctcat gctccatcgt aataagaaac ccaatgatgt ccatgaaggg
     4621 aaatggattg gtgtgggtgg taagctagag agaggagaga cgccccagga atgcgcggcg
     4681 cgtgaaatcc ttgaagaaac agggctcaaa gccaagccag ttctaaaagg tgtcatcact
     4741 tttcctgaat ttacaccaga tttagactgg tacacctatg tttttaaggt gacggagttt
     4801 gagggcgact tgattgactg caatgagggg acgctagaat gggttcccta tgatgaggtt
     4861 ttgagcaagc cgacttggga aggtgaccac acctttgttg agtggctttt agaggataaa
     4921 cccttctttt cagccaagtt tgtttatgat ggggataaat tgttggatac ccaagttgat
     4981 ttctatgaat aaaggagaaa gcagatgcta ctaatcaaaa atggtcgtgt aatggatccc
     5041 aagtctggtt tggatcaagt ttgtgatgtc ttagttcaag atgggaaaat tatcaaaatt
     5101 gcgcctgaga tcacggaaga aggagcagaa acgattgatg ctactggtct tgtggttgct
     5161 cctggtttgg tcgatattca tgtgcatttc cgtgaacctg gtcaaacaca taaagaagac
     5221 attcatactg gtgccctggc agccgctgca ggtggtttta ctactgtcgt catgatggct
     5281 aataccagtc caaccatttc agacgtggag actttgcaag cagttctcca gtcagctgcc
     5341 aaagagaaga ttaatgtcaa gacagttgcg accattacta aaaactttaa tggtaaaaac
     5401 ttgactgact ttaaggcgct cttagaagct ggtgcggctg gtttctctga tgacggtatt
     5461 ccgcttgaga gcagtaagat tgtcaaggaa gccatggagg aagccaaaaa attcaatacc
     5521 tttatcagcc ttcatgagga agatccaggt ttgaacggtg ttcttggctt taatgaaaat
     5581 attgctagag aacatttcca tatctgcggt gctactggag tggctgagta cgctatgatg
     5641 gcgcgtgatg tcatgattgc ctatgcaact aaagcccatg ttcacatcca gcatttgtct
     5701 aaggaagaaa gtgttaaagt agtggagttt gctcaggggt taggtgcaga agtcacagca
     5761 gaagtagcgc cacagcattt ctctaagaca gaagcacttc ttttaacaca aggtagcaat
     5821 gctaagatga atccaccgct tcgtttggaa tcagaccgtc gtgccgttat cgaaggtctc
     5881 aaatcaggtg tcatcacagt tattgcgact gaccacgcgc ctcatcatgt agatgaaaaa
     5941 aatgttgagg atattaccaa agcgccatct ggtatgactg gcttagaaac atccctgtct
     6001 ctcagcttga cctatttagt agaagctggt gagttgagct tgatggaatt acttgaaaaa
     6061 atgacataca acccagccaa gctttacaac tttgaagcag gttacttggc tgagaatggt
     6121 ccagcagata tcactatttt tgatgccaag gctgaccgct ttgtggactc ccattttgct
     6181 tccaaagcag ctaattcacc attcatcggt gaaaccttaa aagggcaggt taaatatacc
     6241 atctgtaagg gacaaatcgt ctatcaagct tgataggagt tagaatatga attattttcg
     6301 aaaacgtagg gagagacaag cgaaatctaa tagtggaatt tactgtcccg cagctaatcg
     6361 ttccaaaatt caatatgaaa ctaaagaaaa agctgaccat gcggttcaat atgataacgg
     6421 aggtttagtt agaagctatt attgtcgtac atgtgcgtgt tggcatacta cttcaaaatc
     6481 taataaacct ccgctaagtt tgaaaaacta tggtctgaaa ctctttggtt tagataaatc
     6541 agaatagaaa gagaagtatg tataagacaa agtgtttacg agagaagtta gtattatttt
     6601 taaaaatttt cttcccaatc ctgatctacc aatttgccaa ttattctgcc tcttttgttg
     6661 atactgcaat gacaggtcaa tacaacacta tggacttggc tggtgtatct atggcaacca
     6721 gtatctggaa tcctttcttt acatttctaa cagggattgt gtcagccttg gtgcctatca
     6781 ttggtcacca tcttggtcga ggcaaaaagg aagaagttgc gtctgatttt taccaattta
     6841 tttatttggc cttgggccta tctgtggtct tgctggggat ggtacttttc ttggcaccaa
     6901 taatcttgaa tcatattggg ttagaagcag cagtagcggc agtagcggtt cgctatcttt
     6961 ggtttttatc tatcgggatt atccccttgt tgctctttag cgtcattcgt tccttgctgg
     7021 attcgctggg cttgaccaaa ctgtccatgt acctcatgct tttgttactc cctctcaata
     7081 gcggatttaa ctatctcttg atttacggtg cctttggtgt tccagaactg ggaggggctg
     7141 gtgctggttt aggaacatcc ttggcctact gggtcttgct tgggatttct gttctggttt
     7201 tatttaaaca ggagaagctc aaagccttac accttgagaa acgaattcca cttaatatgg
     7261 ataacattaa ggaaggagtt cgtttaggtc tgcctattgg gggaactgtc ttcgcggaag
     7321 tggctatctt ttcagtggtt ggcttgatta tggctaagtt ttcgtccttg attatagcta
     7381 gtcaccagtc agctatgaac ttttcaagtc ttatgtacgc ctttcctatg agtatctcat
     7441 cggctatggc tattgtcgtt tcctatgaag tgggagccaa gcgatttgat gatgcgaaaa
     7501 cctatattgg tctaggaaga tggactgctc tcatttttgc ggccttcacc ttaaccttcc
     7561 tttacatttt taggggaaat gtggccagtc tttatggtaa cgacccaaaa tttatcgatt
     7621 tgacagtgcg ttttttaact tatagtcttt tcttccagtt agcagatacc tttgcggcgc
     7681 cgcttcaggg aattttgcgg gggtataagg atacagttat tcctttttac cttggtttgc
     7741 ttggttattg gggcgtagca atccctgtgg ctacgctatt tgattcccta acagattttg
     7801 gagcttattc ttactggatc ggcttgatta ttagtttgat tgtgagcggg gcgctctacc
     7861 gttggcgttt aactgtgatt atgaagagat ttgaatcttt agcaaaatcc aaatactaaa
     7921 aagtttgtga aaaaatattc ttgacaagaa aatcccttgt tttcacgggg ttttcttttt
     7981 tattttgtga aatttctttt agaaaaactt tgacagtatt tgccaaaagc ggtaaaatag
     8041 taaggtaaga agaaagttag aaaggcaaga agatgtcaac tttagataaa aatcttttgc
     8101 tagaaatgtt ccgtaagatg gaagaaatcc gtcgcatgga cttaaaaatt gcacaattag
     8161 taaagaaagg gaaagtgcca ggaatgacgc acttttctgt tggtgaagag gcagctaacg
     8221 tgggggctat gttagctctc aatccagatg atctgattac ctcaaaccac cgtggacacg
     8281 ggcaagctat tgctaaaggg attgacctca acggaatgat ggctgaaatc cttggtaaat
     8341 acactggaac ctgtaaaggg aaaggtggat ctatgcatat cgctgacctt gatgctggga
     8401 accttggtgc caatggtatc gtaggtggtg gtatggggat cgctgtcggt gcagccctca
     8461 gtcagcaaat gcaaaatacc ggtaaaatcg ttgtctgctt ctttggagat ggtgcgacca
     8521 atgaaggtgt tttccacgaa gcagtgaaca tggcttctat ctggaacctg ccagtcattt
     8581 tctattgcat taacaacggt tacggtatct ctgcggatat caagaaaatg accaatatag
     8641 aacatatcca tcaacgtagc gccgcttatg gaattcctgg aatgttcatc gaagacggta
     8701 acaatgtcat cgatgtctat gaaggatttc agaaagctgt ggaccatgtt cgcagtggca
     8761 atggtccagt cttgattgaa agtgtaactt atcgctggct cggtcactca tcatctgacc
     8821 ctggtaaata tcgtacgcgt gaagaagtgg aattgtggaa acaaaaagat ccaatcgaaa
     8881 acctccgcaa ttaccttatt gaaaataaca ttgcaagtgc cgaagaattg gaagaaatcc
     8941 aagcgcaagt aaaggaagca gtagaagctt ctgttaaatt tgcagaggaa agcccattcc
     9001 caccgcttga atcagccttt gaagatattt acgcagacta aggagaaaga gataaaatgg
     9061 aaacaaaaac aatgtccttc cgtgacacca ttatccttgc tatgtctgag gaaatgcgtc
     9121 gcgatgaaaa tgtgttcttg atgggagaag acgtcggtgt cttcggagga gacttcggta
     9181 cttctgttgg aatgcttgaa gaatttggtc cagaacgtgt ccgtgactgt ccgatttctg
     9241 aagctgccat ctctggagca gcagcaggag cagccatgac aggacttcgt ccaatcgtcg
     9301 atatgacctt catggacttc tctgttattg ccatggacaa tatcgtcaac caagctgcta
     9361 aaacacgtta catgtttggt ggtaaaggtc aggttccaat gactgttcgt tgtgcagctg
     9421 gtaacggagt tggctctgcc gctcagcact cacaatctct agagtcttgg tttactcaca
     9481 ttccaggact taaggttgtg gcacctggaa cacctgcgga catgaaaggc ctgcttaagt
     9541 cttctatccg tgataacaac tcagttatta tacttgagta caagtcagaa tttaaccaaa
     9601 aaggggaagt gccagttgat ccagactaca caattccact tggggttggt gaaatcaaac
     9661 gccaaggaac ggatgtaaca gttgttactt atggaaaaat gcttcgccgt gtggttcaag
     9721 ctgctgaaga attagcagaa gaaggaattt cagttgaaat tgttgaccca cgtacccttg
     9781 ttccgcttga taaggatatc atcataaact cagtgaagaa aactggtaag gttgttctgg
     9841 tcaacgatgc ccacaaaaca agcggctata tcggtgaaat ttcagctatt atttcagaat
     9901 cagaagcatt tgactatcta gatgcaccaa tccgccgttg tgcaggagaa gatgtgccga
     9961 tgccttatgc acaaaaccta gaaaatgcaa tgattccaac agttgaaagt atcaaagatg
    10021 caatccgaaa aacttataac aaagaatagg gttacataag ataagttatg taaataaaat
    10081 aaagctgacg agaaactttg tatcttttag gtgcagagct cttcgtcatt aatatcttag
    10141 attagagcac gggctaaaag atcggaaaaa agataaatct ccttggcttc atcgaagcca
    10201 tcgtcgattt cctatttttc agtcgctttt gacgcccttt gtatcataaa tagaattgga
    10261 gaggtcatgg ctgatgacaa gctaagagcg actcctgcag ctagaaagtt agcggatgat
    10321 ttagggataa acctctacga cgtttctggc tcaggtgcaa acggtcgtgt ccacaaagaa
    10381 gacgtggaaa cttataaaga cacaaacgtg gttcgcattt cgccacttgc aaaacgaatt
    10441 gccctcgaac ataacattgc ttggcaggaa atccaaggaa ccggtcatcg tggtaaaatc
    10501 atgaagaagg atgttttggc cctgcttcct gaaaatatcg aaaacgatag catcaaatct
    10561 cctgctcaga ttgaaaaagt ggaagaagtc cctgataacg taacaccata tggtaaaatc
    10621 gagcgtattc caatgacacc aatgcgtaag gttattgccc aacgcatggt tgaatcttac
    10681 ttgactgcgc caaccttcac cctcaactat gaagttgata tgactgaaat gttggctctt
    10741 cgtaagaagg ttcttgagcc aatcatggaa gcaactggga agaagactac tgtaacagac
    10801 cttctttcgc ttgcagttgt taagactctt atgaaacacc catacatcaa cgcttcattg
    10861 acagaagatg gcaagactat tatcactcac aactatgtca atcttgccat ggcagttggg
    10921 atggataatg gattgatgac acctgttgtt tacaatgctg agaagatgag tctttcagaa
    10981 ctggttgtag cctttaagga tgttattggc cgtaccttgg atggtaaatt ggctccaagt
    11041 gagttacaaa attcaacatt cacaatcagt aatttgggaa tgtttggtgt tcagtccttt
    11101 ggtccgatta ttaaccaacc caactcagct atccttggtg tcagttcgac aatcgagaag
    11161 ccagttgtcg tcaatggtga aattgtgatt cgcccaatca tgagtttagg attaaccatt
    11221 gaccaccgtg tcgtagatgg tatggctggt gctaagttta tgaaggactt gaaagagttg
    11281 attgaaacgc caatctcaat gttaatttaa gagcaagagt attcattttt aagatataga
    11341 taaaggaagg aagacatggc cttagaagta attatgccaa aagccggcgt ggatatgaca
    11401 gaaggacaaa tcgtccaatg gaataaaaaa gtcggagaat ttgtaaaaga aggagaaatc
    11461 cttttggaaa tcatgactga taaagtcagc atggaattgg aagccgaaga agatgggtac
    11521 ttgattgcca ttctcaaagg agatggtgaa actgtccctg taacggaagt tatcggttac
    11581 cttggtgaag aaagggaaaa catcccaaca gctggagcag catcgccaga agctagctct
    11641 gtacctgtag caagtacctc aaacgatgat gataagagcg atgatgcttt tgatatcgtt
    11701 gtgattggtg gaggtcctgc tggttatgtt gcagccatta aagctgccca attcggcggt
    11761 aaggttgccc ttgttgagaa atctgaactt ggtggaacct gcttaaaccg tggatgtatt
    11821 ccaaccaaga cctaccttca taacgctgaa attattgaaa atatcggtca tgctgcaaat
    11881 cgtggtatcg tgattgaaaa tcctaacttc actgttgata tggaaaaact tttagaaact
    11941 aaatctaaag ttgttaatac tcttgttggt ggtgttgcag gacttcttcg tagctacgga
    12001 gttactgttc ataaaggaat tggtacaatc actaaagaca agaacgtctt ggtaaatggt
    12061 tctgaattgc ttgaaaccaa gaaaatcatt cttgctggtg gttcaaaagt cagcaagatc
    12121 aacgtccctg gtatggaatc tccacttgtc atgactagtg atgacattct tgaaatgaac
    12181 gaagtgccag aaagccttgt tatcatcggt ggtggagttg tcggtatcga actcggtcag
    12241 gccttcatga catttggttc aaaagtgact gttatcgaaa tgatggaccg tatagttcca
    12301 gctatggatg tggaagtttc taagaacctt cgcttgattc ttgagcgtaa aggaatgacc
    12361 atcttgactg gtactaaact gcaagaaatc attgaggaaa atggtcaact tcgtatcaag
    12421 gttgaaggaa aagacgatat catcgcaagc aaagctcttc tttcaattgg tcgtatgcca
    12481 gaccttgaag gtattggaga ggttgagttt gaattggatc gtggttgtat caaggtcaac
    12541 gaatacatgg aaacttcagt tccaggcatt tatgcaccag gtgacatcaa cggtactaag
    12601 atgttggctc acgcagcttt ccgcatgggt gaagtttccg ctgaaaatgc ccttaaagga
    12661 aatcatgcag ttgccaaatt gaatttgact cctgcagcca tctacactct ccctgaagta
    12721 gcagcagtag gtttgacaga agaacaagcc cgtgagaaat acgatgttgc catcggtaag
    12781 tttaactttg ctgctaacgg tcgtgctatt gcatctgacg cagctcaagg tttcgtaaaa
    12841 gttatcgctg ataagaaata cggagaaatc cttggtgtac acattattgg tcctgcagcc
    12901 gcagaattaa taaacgaagc atcaagcatc atcgaaatgg aaatcactgt tgaggaaatg
    12961 ctgaagacca tccacgggca cccaacttac tctgaagtga tgtacgaagc gtttgcagat
    13021 gttctaggaa tggccatcca ttcacctaag aaaaaataaa acgaagatag attagactgg
    13081 aaggttattt ccagtttcta tcatataaag taaagggata tcatgaaata cattatcaat
    13141 cattcaaatg acactgcttt taatattgcc ttggaagaat acgcctttaa acaccttttg
    13201 gatgaggatc aaatcttcct actttggatt aacaagccat ctatcattgt tggtcgtcac
    13261 cagaacacta tcgaagaaat caaccgtgat tatgttcgcg aaaatggtat tgaggtagtt
    13321 cgccgtatta gtggtggtgg agctgtttac cacgatttaa ataacctcaa ctacacgatc
    13381 atctccaaag aagatgaaaa taaggcattt gacttcaaga gcttctcaac tccggttatc
    13441 aataccctgg ctcaacttgg ggttaaagct gagttcacag gtcgtaatga ccttgagatt
    13501 gatggtaaga agttctgtgg caatgcccaa gcctatatca acggccgtat catgcaccac
    13561 ggttgcttgc tctttgacgt tgatttgtca gtcctcgcaa atgcccttaa ggtttcaaaa
    13621 gataaatttg aatcaaaagg tgtgaaatcc gtccgtgccc gtgtaaccaa tattatcaat
    13681 gaattaccaa aaaaaatcac agtcgaaaaa ttccgtgatt tactcttgga atacatgaaa
    13741 aaagagtacc cagagatgac tgaatacgtc ttttcagaag aagaattggc cgaaatcaat
    13801 cgcatcaagg atactaagtt tggaacttgg gactggaact atggtaaatc acctgaattt
    13861 aacgtccgtc gtggaatcaa attcactagt ggtaaggttg aagtctttgc taacgttact
    13921 gaatcaaaaa tccaagacat caagatttat ggtgacttct ttggtattga agacgttgct
    13981 gcagtagaag atgtccttcg tggggtgaaa tacgaacgcg aagatgttct taaggcgtta
    14041 aaaaccattg atatcacacg ctacttcgct ggtattagcc gtgaagaaat cgctgaagcg
    14101 gtagttggat aaatcaaaaa gaagttggcc aactaaccaa cttctttttt tgatatttat
    14161 ttacataatt taaaatacgt aaaatcataa actatccaga gcattctttt gttcatcatt
    14221 aacaatatgg gtatagaggt cagtgacttg tgtgctggca tgtcctagtt ggtgactgac
    14281 taaaacttgt gatttagtag catcatagag cctagttgct agtgtatggc gcagtttatg
    14341 gggtgttaca cgtactttaa aatcctctga gtatttagca accattttct caacgctaga
    14401 agcatcgata cgattaggaa cacctctgta gagagttaaa aaaagggctg tatctgtttt
    14461 ttccgtttta tagcgttgat tccgaatggc cagataattc tctaaataag gtttagcaaa
    14521 agcagcgaca ttgactgagt cacgtttgcc accttttcga gtaacatcaa taaccatcat
    14581 ttttagattg agatctctta gatctagatt aacagcttca gataagcgaa caccagatgc
    14641 caagagaagg gcaataatgg ctaaatctcg ttctttattt ttgttgaatg atgagagagc
    14701 tcgatttgaa agctgttgtg ggtgctcttg atcgatataa gttagaaaac cttctgtttc
    14761 atcacctaga aagagttttt gcttgatatt ttcagctctg gcagcaaggg tttctttctt
    14821 tttcttggtt gaaacttttt tcattacatt acgatagaaa taaggttccc cctgatcgtt
    14881 ttcaacctcc tcggttagat acttgtaaag actagaaagt gctgataagg ttcgattgat
    14941 agttgtctgt gaaacacctt gttttgttgt attagcattc agcaagggac gttcacgtag
    15001 ataaaggata aaggattcca tgtctttctt agacatattt tccaaaactg ataaaggaat
    15061 atcggatatt ttatcagcgt ttgaaatacc agactccaaa acccagctga aaaatcggtc
    15121 atattcctta aggtattcgt acagggttgt aaaactgtag ggcacagcca gtttagattg
    15181 gtagtattcc agaacatacc agggcatgag ttgttttagt ttgtcgattc gttccagtaa
    15241 aatctcacgt ttcatgtatt ttctccataa ttaagtatac tagtagtata gcatagactt
    15301 gtatattttt caagaaaatt atagtttttc agaaagatgt tataggagtt tttcaaagtg
    15361 ataaaaagac ttagacctac aaacaaaaca gaataaggaa aataataaaa atctcaccca
    15421 ctaaacttct gttaatacta cataactgaa gagctatata aggagctatg agatacaaaa
    15481 cacattgtat aatagattga aactagaata gtacatctgg acttctaaaa cattgctaga
    15541 aattgatttg attgtcctga ttgttttatc ctatttctat ttcatttctc tatataattg
    15601 ctattgtttt ctttttcatg attttaatct tactgtacag atttgataca gtaaactcct
    15661 ttctagcttt atactttatc atttctaact taattatagt cttattttta ataaagttca
    15721 attatttata tgtaaaattc ctcatcagta aaaaataacc gatctcattt ctgagaatcg
    15781 gttttctaaa taaatcaaac tgatcattta cataacataa attatgtaaa tgatcatact
    15841 acaacaacaa atctttgact tttccaattt cactttttgg aataaccagg tgaatcatat
    15901 cacccagata cattctggtt gagccgttaa ctgtttggct cttgccatta tggacttgag
    15961 ttgtgatgag gacgttgtgt ggtaagttga gttcatgaac ttgtttccca gcaattttat
    16021 cagaaactgg tatttcgata agtgtaactt ctccttcgct agatacttct tctggaagca
    16081 ttttttccag catggcttca tagactggcg tacctttgag caaatccatg ataatataag
    16141 aaacaagagt gacaagacca agtggcataa ggttgcgaat atctcctacc atctcagtta
    16201 cgaggatcat agcggttaag ggagcttttg atatggctcc aaaatagcca ctcattccta
    16261 gaatgacaaa tatagggaat tgctcttgac tgacaagtcc aagattgaca cagataacac
    16321 caactaaggc accaagcaaa gaaccaagag ctaaaatggg gaggaaaatt cctcctggca
    16381 gtccacttcc atagctaatc atactccaaa taaagcggat taaaaagtaa gctaataaaa
    16441 cttggaaact aaaattttgt tcagttaaag aaaggacaag ctgatttccg ccaccaatga
    16501 tttgaggtaa gaagattccg actggtatga taaggataaa agccaagatg ggataataag
    16561 ccctatccaa atggattttt tgaccaatca agtcataaac tcttccaacg tttaatacag
    16621 ctttctcata gagaaaacct gaaaatccaa ggaaaattcc catgacgaga tatatccaat
    16681 actgatctag ggtcatggga ggaatgttat ctggcatatc caatactggt gtcaaaccga
    16741 acatgagtag agacacaaag tttgctacga tgctggctgc tagagttgag acccagaaaa
    16801 agcgcgaaaa atggtgatag acttcttcta caacaaagag aagtgctgca ataggagcat
    16861 taaaggctgc ggctaaacct gctgcagctc cactggcaat caaggaacgt tcctctactg
    16921 gactggattt gagccacttg gcaattcctt taccaccaac tgctccaagt tgaatactgg
    16981 gtccctctcg acccagcatg agtccactgg caatagcaag aatacctagc acatattttt
    17041 tccaaagaat gccccaccag ttgagggaca tgaggccttt cagttcggct tcgacttgag
    17101 gaattcctga gcctttaata tctttttctg accgtgttag tttggcactg agccaacaga
    17161 tgagtatata aaacaaaacc agtacaaaaa gattgcgcac taagtaccct tgatcttgat
    17221 aaactccttg tatcaggtgg aagccctttt caattaagaa acgaaaggat ccgacgataa
    17281 ttccaacgat gaggccgaca atgattcctc gaccaacttg ggatagtata gtgctggatg
    17341 caaaggcgaa ttctttctta gaacggacta tttccgactg ttcctccatg gtatttcctt
    17401 tcaattaact ttctttttta cctaaaacca gtgatttaac gggttcaaag ctggttcggt
    17461 gaattggggt aactcctagt tttgtgaggc cttccagatg tttagctgtg ccatatcctg
    17521 cattagtagc gaaatcatag ccagggaact gctgatcgta ttccttcatc aattcatcac
    17581 gtgttacctt ggctactata gatgctgctg cgatagagag ggagttggca tctcctttga
    17641 taatggaggt ttgtgaaatg ggcaagtcca gtttcatggc atcaatcaaa aggtgctctg
    17701 gttgagggct gagctgggag attgcttctt gcatggctag tttggttgct tcatagatgt
    17761 tgacttggtc gatgacctga ttatctatga taccaattcc aatcgacaag gcttggtctt
    17821 gaacggcttg gaaaatctcc agatgtttct ttttaggaat tttcttgctg tcgttgagac
    17881 ctttaatctt acaattttta ggtaaaatga cggccgcagc gactacagga ccagcaagag
    17941 gaccacgacc aacctcatca atacctacaa ttaaggtcaa tccttgctta taaagttctt
    18001 tttcataaga aagcatggat tccaagcgca aattttcatc taattcagct tgaatggctc
    18061 ttttacgctt gctgatttcc ttttgaactc ctgagcgatt atccttttca agctctaaaa
    18121 aaatagggct ttctaactcc ttgactgtca caaggaattc tttgatttct ttaatcgttg
    18181 ccatcgaggt cttccaatgt atctaaggta tagttaccga gtttgccatc acggacttcc
    18241 ttcacgaaga gactgtaaaa acggtcatag tcatcacgga aaccgagggc gcgggtcata
    18301 tccataataa tcacaggcgc ttcttcttca attttcattt gtttgaagcg ttcagccagc
    18361 ttttctggat aatgttcttt gaaataattg ataccaaaaa tggtaacctc atccatagga
    18421 agcaactgat ccttgatagc tccagtcaat gccaacttaa gtgcaacagt ttcatcctca
    18481 aacttaggcc agagaatccc cggtgtatcc aagatttcca ggtctttatt ggttttaagc
    18541 cattgttgac cttttgtgac ccctggcttg tttccaacaa cagcaatctt tttaccagcc
    18601 aaacggttca tcagagttga tttaccagcg tttggaatcc cgataatcat agtacgcaag
    18661 gtttcaatct gaatcccacg ttctttctgg cgagcaatct tatcagccat gagcttcttg
    18721 gccgcatctg ttacaacttt tacagtcact tgctccttgg agttgatagc cagagtttgg
    18781 attccttgtg attcaaaata ctgacgccat tccttggtca ttgctggatc agccaaatcc
    18841 gccttattta aaatcaagag ttttggttta tcaccaacaa tcttggtcaa cataggattt
    18901 tgactagata gaggtaagcg tgcatccacc aaaatcgtta caaaatcaac aaattttaaa
    18961 ttttcctgca cctgtcgacg cgctttagac atgtgaccag gaaaccattg aatagtagcc
    19021 attgagtttt tttcctttcg tagcaagggt ttagagatcc tattttattt tactatcgtt
    19081 tgaacataaa gggttctcaa atgaatgatg ttcaaacagt tttttgtttt atggcataga
    19141 aatctacctt atattatatc atgccttctt tattttgtcc gatgatagcc tttcaagtac
    19201 cctatataga aaaaagccat caaatgatga cttttcagag ggagcaaaaa actgttcctt
    19261 ctatattcta aacatttaaa tgtcttcttt aaacagttta ttaaagaaac cgtctgtgtt
    19321 tttaagaagg gctttgaaga ccttagattt aagagcaaca gtgtgagaga agtcatctgg
    19381 tttcttcaaa tcttcttcaa ctgctttatc aaataaaggc cttaactcat catagttact
    19441 gatatggacg ccatcaatat cgatagcctc aaaacctttc ttagctttag caagaacttc
    19501 cccgtaccaa tgtctcttcc actcttcaag agtattaaag tttcctttcg atactttatt
    19561 aattattagc ttatcgctta gtaaaccgtg gttgttttta tctgcttctg ctttgtattt
    19621 atttgataca taactaataa agccattttc ataaccataa tagccccaca tacggaaagc
    19681 attgtgctta aatgataatg aaccaggagc accttgactc gtattcccac cgtaaattcc
    19741 tgccatcata ttgacattta cgtaagctga gtttggtgta aaatcttctg gtctataacg
    19801 tccattacct ggatttccat gttttgtcat gaagttatta tccactaaat cattgacgct
    19861 agataaagtc attacctttt cttcatcttt caaatcacgg atactatccc attggtttgg
    19921 acctaaaaga tcattatact gaatcttaga gcgcatttct ttgtcaatct tcttgaacca
    19981 cttactattg tctcctttaa ttttaggaag tacagcatca gcttctacat gatcaagcaa
    20041 catcatagct tcattgtagt ttctcatgta gcggtcaatg tcatcacgtg tctttaattt
    20101 gtcaggatct gggttgtacc attggttacc atcatttgga cgatggtaag ccatgttaat
    20161 tccaagtgcg ccgtattcac cattagtcgt tgagctgtca ggtgtttgca acataccttg
    20221 agcataggct tcaacgtctg taccttgacg atgcttatga ccaccgaagt aaagcattct
    20281 atcgtttacg tgtgttgttt cgtgagtaaa ggctgaaata ccaaatgggc taatcatatc
    20341 cgtcaccata aacaagacct tgtcatcatt atttggatta gggaatattg atgccatagc
    20401 gcccatacgc cagtctctat catggtgtcg ccatgttgga ccatataatt cacgaattgg
    20461 agctataggg ttacctttac tgtcaaatcc tcgacgacct acaggagaac cattgtaggt
    20521 ctggttatcc caaactggtg tcggtaccat attttgactc ttaagaagtc tatcacgaac
    20581 attaggcaga gctaatctgg accagaaatc taaataagtt tgttgagcat gtgcaacttc
    20641 atcaatctta gttttgaact cattacgagc ttcttctgta tgttttccat atttctcaaa
    20701 tgaactgtac gccatcgtgt tatatgttga aattaagaac atatgggcat ctttaagcgt
    20761 tagaagtggt aaaatcatac gaccatgaac accgttatct aaaccatcga acacacgatg
    20821 acgtttatct ttcaactctg tatttgttgt cttaggttct gaaacataaa cattcttagc
    20881 tgagtggata aaccaatcat tgatgtcttt atcctctgta aacagacgca tgttgtattc
    20941 taagaagtta cgcaaattac ttttaccagt attacctgcg atgatttcac ggtaagcatc
    21001 ctgagtccga tcccctttca agttcttttc acttgaaccg atgttgatga gtctgtcaat
    21061 aacacttggt gttttaccat aaaaatcagg tttgaacatc atgatgtttt ttatacttat
    21121 gtcaccgtat ttgatatcat agtaacgatt gagataagtc agagccatca tgattttagc
    21181 tttattggtt tccactttct tgagaagtga ttttatcgca gcttcatcac cattcaattg
    21241 atggtcttca ttttcgacca aggatttgac taacttatct agattttgct tcacttcggc
    21301 aaagctttct tctaagtata gatctttaat ataaccattc ttacgttcat ccgtattctc
    21361 tgcaggttta tcacgtctac tcattaaatc tcttacttct tgtgaaatca attccacact
    21421 tgataattga gatttaacat cagcaatcaa tttatcacga tttttaacaa ccatattcgg
    21481 tgtataaaca acatcaccaa gaccagatac actatattca cgcacttgtt ggactttaga
    21541 atcagtattc ttagtaacat ccatttcttc cttagtacca tcagcatagt gaaccataat
    21601 cttatcaata tctgataaat cggtcacgaa ttgaccatcc ttcatccccg taacagaaag
    21661 gacttctttc ttagctaagt ttgaatcttc tgcaagtttg ttaccttggt tgacaatcca
    21721 ttctttatta taaaatggtt gaagtttctc aatattacga taagctaaat cacgttcgga
    21781 tttgtagtct tgagttgact tatattcatc ttctcttctg gttaaacgat ttaagcgatt
    21841 gaccacaggt tcgttgattt cgtatttatc agcagtaagt ccaaaatttg caattctctt
    21901 attagcttca tcttgactaa tttctttaat tttgtcacga gaacgtttgt atgaatgctt
    21961 acctgaactg acacctttga caacaaagtt acgctctaac cagccatttg tgaaataacc
    22021 accatcgtag tccaaatcgg aagaaccaaa gaactcttca ccattttcaa ctctcatcat
    22081 tgaaatatta tcttcaagtt taccaaaagg aagattgcta ttgataaatc cacctgtttc
    22141 aaacaaaact ttagtcttaa tggttccttt ggtgactgat ttacgaattg aaccgtattt
    22201 accaattccc atagagtcgt taccattatc tgttttagca actaaaccac ctatacgggc
    22261 tctgttagca acaatatcag cctcgacata tgcttttgca agattacctc tccaaatctc
    22321 accggcaatg ccagccatat attgaccttt atcaccaaca cctgtaagat taccgatgaa
    22381 ggctacattt gtcaactgac caccactatc gattttattg acgatacccg caatattgtt
    22441 attagcaact acattaccag ttaccttaac atcttcaaca gttgcatttt tcaccatatt
    22501 ggcaacaggt gctattccgt cagcccaagg catattgata tccacattac ctaaattaat
    22561 atttttcaca gaaccatttt caacacggtc aaataaggga cgggcaatgt tatgaattgt
    22621 atattgttta ccatcaacac ttgatagatg tcccttaaac gtacctggaa catattgttt
    22681 gttaggtgtt ggtacattcg ttgcattcaa atctgctccg attttaaatg taccattttt
    22741 atcagcattc atggcatcta ctaactcttt aaagttataa taaacgtcat caacttttgg
    22801 aactggtttt tcaatataat gaacatactt gttacgaacc ttcgttgggt ctgtatgttg
    22861 aatcaaatct tgagcttcgg cagtgatttt ataaagtttc ttaccttctt cagtcacttc
    22921 ttcaatttta tcaactgcta aacgtgttac tttattatca cgactagtaa tttttaagta
    22981 cattttcttc acatcttcat ctgaaggttt ttcagtcata aaatcactag gtgtttcagt
    23041 accatcgtca tttaccttca ctaaattagt agatgcgata tttttaattt caactttttt
    23101 caaatccaag cgaagtggtt tctcttctaa tgttgcagtt tcttctccct tgccacggtc
    23161 ataggtcata gtggtctcta tcttatagtc cttgtagaat tttaaatctt ctacctgctt
    23221 atctaattgc tctgaagtca aatctactgt tttgacaatt tcatttccat ctttaattgt
    23281 cgcagtaatt ttattgattg tgactccatc tgtattaact aaatgataag aaagagttgc
    23341 ggatcggttt aatacatttg tatttgttct atctaaggtc aaaacaggag ccgttttttc
    23401 tttaggtttt tctttggtac cttttacaag aattttaggc ttcatttctt tcaaaatagt
    23461 tactgtttca actgggtcac tgattttttt gccatgtaac tcttcatagc tagtaacaat
    23521 ttgacgttcg ccatcctcac caacttgtct aacagaatca tcgcctataa atttgctagg
    23581 atcagattct tctatagtgg ttttaggaat agtctctgtc cgaatatcgg tatgtaaaga
    23641 aggcaactct tctctttctg atgcaacttc tgaaactgta gatattggtt cagtatactc
    23701 tggaatgtcg ctaacaggtg gctcaatcaa gtttccattt tcatccactc ctgttgtacc
    23761 tactggctca gtatattctg ggatgtcgtt aacaggtggt tcaatcaagt ttccattttc
    23821 atctactcct gttgtcccta ctggttcagt gtatgctagt ttctcatgaa cttcaggctc
    23881 aacaaggttt gcacctattg gttgcgtata gtcaggcttc tcatgaactt ccggttcgcc
    23941 tttctctgtt acaactttgg gttgctcaac tggcgaaaca gttgatgctg gagaaaccga
    24001 cgaaacagtt ttatcatcaa gctccaagta accaacatat ctgtaaccag aaatttgcaa
    24061 cacaccatca cgtccagact catggatgtt agcattcaaa ttaagtgctg aagcagttga
    24121 tagtgtaact aaacctgtcg cccctacaat caaaaatgta gctatttttt tatttctatt
    24181 atcttttgtg ataatcacaa taagactccc gatagctaac aaacccaatg cagtcataat
    24241 agattgagaa cttcctgtgt ttggtagtgc gtctttttca taatccaaag catatgactc
    24301 tttggattca tcaggtctac cttgtttgag ttgatcacgt tcagtttgtg tcaaactact
    24361 ataatctaag taatgataag tcgatgcacc aacaactgac ggtgcaaata aaagactccc
    24421 caagaatacc gaaccaacaa taccctttat tttccgaatg gaaaatttat ctttttttaa
    24481 taatgacaat tctaatcctc caccttcact ttttatttcc taatagtaaa cactattttt
    24541 tattctcaaa aattgaactt ctaaaatcac cggtttggtc aagataagct ttaaagattg
    24601 cttttttcag tttaagaact ctactatttg tctcaggata taatttggca acttcatcct
    24661 cagcatcaaa ttttactgca tcttcaatca ttttttcaag tctattaata ttatcaatag
    24721 tcactttctg ttgtcgagcc caagaaccgt ttggatcgat aaaactaact ttatttaact
    24781 ttccaaattt atcttctctt tctttataca tatctttctt gaaatctatc caagaattat
    24841 actggttatt aaatactttt gaaagaacaa tttcatcagt aactaaacct atttgtttac
    24901 catatgatgt aataactttt ccttgagctt tcgcttcttt ttcatactga ttagaaatat
    24961 atggaaccat accgtctttg taccccttgg ctgccaataa ctcaaatgca atacgtcgtc
    25021 ccattaaatc tcctggtgtt cctattttac tgctcaatgc tgaataaatt ggtgagaata
    25081 gattaattgt atagtagcca ttccgcttat aagttgcttc atctacataa cctcttcttg
    25141 ttataatatc atgttctatc aaactgttaa atgtagttaa tttagtaagt tcttctggtt
    25201 ttaatctacg tacaacattc gttgcataaa cagaatttcc atcaggatca ggtttatatt
    25261 cattttctat ttttcttaat aaatcatatt tttgatttgt atctaattta gctacagctt
    25321 taccttctag tatttcaagc atgtatatta catcaaacat attatgcata tacttattta
    25381 gatcatcaac acttccaaat cgttgagttg gatctgctac ttgaagcctt gttgattctt
    25441 ctgattgatc atattttaat atggaattaa tagtgactgt tggatcatta ggatgatcag
    25501 gagcttgtag cagtccttta gcataaaact ctggtccaag tccatttcgt cttccatagc
    25561 ctccaagata aatttctcta tctgagttat gtgtcatttc gtgtgtataa gttaccgcac
    25621 catctttatc aagcatgcga tatgccatat aatagaatgc atcccctgta gcatatgctc
    25681 cattagaatt atgaacaaca ttatttcccg ctggaccgaa gaaatgtttt atagctggat
    25741 gattagtttc aaaagttgct tgatatgtat ctttatcttc tttttcatct gtaccaaatc
    25801 taaatgcatc atagactaga acacttctat ataatttttc tttattttta tcatctagta
    25861 ttctatacca ataatcatag tgatctctaa atctaacagc cgcttcttta gcttttttct
    25921 caacatagtc atttaaatca gttcctgctg gatgttcttt acttctatat ctatcataag
    25981 caccgaaacc tatagtcgaa atgttggcta taataaacac cgatttttca ggtaatgtta
    26041 atagcggcaa caccatactt ggatatttcc aagatgggct agttaatcta tcatatactc
    26101 caattgaata tggactacca gctattgatt gcttatcact tacctcttta atagtagatt
    26161 tttcttctac aatatatgct ttagtttgtt ctttaaacca ctcattattt gttttatctg
    26221 gtacaaatgc ttttctatat ttctctaatg catcaaataa attattagtc ttataatttt
    26281 tcgctaataa aacattataa gttataacat tattttttgc tagtaagtta ttatatcccg
    26341 atttacctag ttcgataaca ttatctaacg ctgaactatt tgatttaccg aagaaatcca
    26401 agtggtacat gactaaatct ttcgcttttg tatcacgata tttgaagtcg taccaacgct
    26461 ctaagtatgt taatcctaga agtaaagctt ctttattatt tttaattttc tcgattacat
    26521 aattatctat taccttattg ttattttcag ctattgcagc atctgcagta agaagttttg
    26581 tcaaactatc tttcaaatta gtttttgtgg tattgaattg ttcttctaaa tataactccg
    26641 ttaaactaac atccttagaa acatctaata cttttctaat tgcctctgat ttatattcta
    26701 cactatttaa tgctggtaat acttcatcta aaatattttg ataattgcga agtaatgtat
    26761 tcggtgtata aattaagttt gtaccgccta ttctatactc tgctacattt gaaaaatcag
    26821 tttgataatt tacattaagt gtctctgatg tattatccgc gtaatatagt aatagtttat
    26881 ttatacttga tttatttttg ttaatatcac taattacttc tttatctttc atagggacta
    26941 ctgacaataa ttctttatta taaagattac tacttgtctc tactaaattt ccgtacttaa
    27001 caattgtttc tctattataa aacggtaata gtttttcaat atttttatat actttttctt
    27061 ttgatgaatc agcattctca agatttttat agtcagttac atatctagaa gagaaattaa
    27121 aatcttctgt tttaatagta tttttagata caaaagtagc ttttttctct tttatttgtg
    27181 catcttctaa tacttctcct ctttgaacag tagaatcctt agttaagatt tcatcatctt
    27241 cttgtactac gttcacaacc ttattatttt tattactgta tgtgccattt gcttttatac
    27301 cattaaaatt ataacctgca atagcattac cattcgttac attaacatcg cttagtacat
    27361 tagaaagtcg accggatttt tctaatcctc caaccggatc ccaaagatta ccaactactc
    27421 caccaacgtt tgcaaaacgt tgaacattat ttagatttcc ttcagcataa ctattactaa
    27481 taagcgcact attttctact gcaccaacta ttccaccaat agactggtct ccttgtgttg
    27541 catttgaaga taaatcgatt gatgcgaacg acttatctat taatcccctc ggaccagaaa
    27601 gtttgcctac taaacctccg atttgataac tagcaactgt tttatatgta ttagttattc
    27661 tacctgtata gctactgtta gaaatagtag aattattaac ttgtgatact aatcctccta
    27721 tgctacgttc cccagctatc gctccatcag catgaacgtt atcaatatga gtattgttat
    27781 ttgcttcttt cgcaagtgtt gcagtatcgt cctttgcgga aatattaaca tcttttagtg
    27841 aaagtttttc tactgtagca ccatttaatt ccccaaataa tggttttttc aaattataaa
    27901 tagcataata tttctcatta tttgttccta ctaatattcc atggaataca ttattcacat
    27961 aactttcttg accatctgga agttcaactt cacgagcatc catagttgct cctaaacgga
    28021 atgtgccatt aggattgcta ttcattgcat ctactaaatt tttaaatgat gtatataccc
    28081 cagtttcact ttgctgagtt tttggtaaat agtatgtata attttctagt acattattat
    28141 tttcatgttg tatcagacta tttgcacttg ctactatctt ataaacatct ctattatctt
    28201 tagtatcatt agtaatgctt gtaactggta gcatcacatc tttaaagttc tctgatttaa
    28261 ctttcataaa gtaattttct gcattacttg gtaaagaagc aagtgaagtt acatgtcgat
    28321 acttattacc atcttttgaa tatagttcta cagaagatat atttctaagt tctaatttct
    28381 taataacttc tttttcatct ttagcgtcat cttttttctc atctttatga cgataaagtt
    28441 catcgtatac tggtggaatt tcattacctg ctataatact cttaatccaa gctaattgtg
    28501 taggagataa aatcaatccg cctgagcgat tttgtgctcc attttgatgc acaccaataa
    28561 cttcaccatt agcattaaat attcctccac cactcatacc aggatttcca ccttgatatt
    28621 gaacaccgta ggtgttattc gcatattctt gttccatttc taccgtagca tcttttaagt
    28681 gcgtatattt cctagctgga tatccatgaa catttagttt gtcattaaga tttactttcg
    28741 aataattttt aactaattct acatgttctc tgccagttgt ttcaggtagc ttaatcactg
    28801 ctaaatcata gataaatcct tttggataat cttttttatt ataaaaatga atattgttag
    28861 cagtaaatgt tcctttttgt ccatcaggag tcacccactc atatacgttt ttagatttat
    28921 cacctctaaa ttcgccagta gtattatcag tagaattatt aatgaaatta tgtgctactg
    28981 ttaaaagtac atttggtgca ataaaagtag ctgatcctac tccagtaacc ttcgacgaat
    29041 ctgtaataaa gttcatatta attaatgcac tagatttttt ctgaatactc atttcttctg
    29101 gtgtattagt attttcatta tttggtttag tatcttctgc actaggttgt tcgattttac
    29161 cagtatattc tttcagtgct tctactttct ctggctcgac tatcgctccg gcttgcaccc
    29221 ctgtgtattc tggtagtggt gctatctgct ctggttcaac aatagcgcca gcctgcaccc
    29281 ctgtgtattc ttttgatggt tctacttttt ctggttctac tattgctccg gcttgcactc
    29341 ctgtgtattc cttcggtgct tctaccttct ctggttcgac tatc
//
LOCUS       DBEJAQ010000027        25947 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.027, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000027 DBEJAQ010000000
VERSION     DBEJAQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.027"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            120..857
                     /locus_tag="ACL297_001236"
     CDS             120..857
                     /locus_tag="ACL297_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGR0071501.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            854..2545
                     /locus_tag="ACL297_001237"
     CDS             854..2545
                     /locus_tag="ACL297_001237"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGR0071502.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            2661..3224
                     /locus_tag="ACL297_001238"
     CDS             2661..3224
                     /locus_tag="ACL297_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HGR0071503.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            3246..8915
                     /locus_tag="ACL297_001239"
     CDS             3246..8915
                     /locus_tag="ACL297_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGR0071504.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPNRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEALKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            8976..10697
                     /gene="pabB"
                     /locus_tag="ACL297_001240"
     CDS             8976..10697
                     /gene="pabB"
                     /locus_tag="ACL297_001240"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGR0071505.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            11228..12343
                     /locus_tag="ACL297_001241"
     CDS             11228..12343
                     /locus_tag="ACL297_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGR0071506.1"
                     /translation="MSKGDFIDPKTQFKHQYRGESPVFRIRNRKYVELGTTDKKLDER
                     RDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSVNSNLLNSFGEKAKKVLIRSKL
                     INVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDLTDGEYKYVAYESQQYTDPQTT
                     LTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPKPDEKPKPSAPQQEKTKPTVQS
                     GWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYVQNAWVGNYYLKSGGYMAKSEW
                     IYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAKSEWVYDSNYKSYYYLTSEGSY
                     ARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK"
     gene            12430..13349
                     /locus_tag="ACL297_001242"
                     /pseudo
     CDS             12430..13349
                     /locus_tag="ACL297_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
     gene            13484..14443
                     /locus_tag="ACL297_001243"
     CDS             13484..14443
                     /locus_tag="ACL297_001243"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HGR0071507.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            14540..15379
                     /locus_tag="ACL297_001244"
     CDS             14540..15379
                     /locus_tag="ACL297_001244"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGR0071508.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(15427..15597)
                     /locus_tag="ACL297_001245"
     CDS             complement(15427..15597)
                     /locus_tag="ACL297_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HGR0071509.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            15718..16602
                     /gene="miaA"
                     /locus_tag="ACL297_001246"
     CDS             15718..16602
                     /gene="miaA"
                     /locus_tag="ACL297_001246"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGR0071510.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            16595..17833
                     /gene="hflX"
                     /locus_tag="ACL297_001247"
     CDS             16595..17833
                     /gene="hflX"
                     /locus_tag="ACL297_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGR0071511.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            17826..18449
                     /locus_tag="ACL297_001248"
     CDS             17826..18449
                     /locus_tag="ACL297_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGR0071512.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            18464..19393
                     /gene="rnz"
                     /locus_tag="ACL297_001249"
     CDS             18464..19393
                     /gene="rnz"
                     /locus_tag="ACL297_001249"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGR0071513.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            19414..20169
                     /locus_tag="ACL297_001250"
     CDS             19414..20169
                     /locus_tag="ACL297_001250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGR0071514.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(20223..21131)
                     /locus_tag="ACL297_001251"
     CDS             complement(20223..21131)
                     /locus_tag="ACL297_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGR0071515.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            21195..21464
                     /locus_tag="ACL297_001252"
     CDS             21195..21464
                     /locus_tag="ACL297_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HGR0071516.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            21464..21844
                     /locus_tag="ACL297_001253"
     CDS             21464..21844
                     /locus_tag="ACL297_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGR0071517.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            21933..22160
                     /locus_tag="ACL297_001254"
                     /pseudo
     CDS             21933..22160
                     /locus_tag="ACL297_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22200..22925)
                     /locus_tag="ACL297_001255"
     CDS             complement(22200..22925)
                     /locus_tag="ACL297_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HGR0071518.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            23087..24928
                     /gene="typA"
                     /locus_tag="ACL297_001256"
     CDS             23087..24928
                     /gene="typA"
                     /locus_tag="ACL297_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HGR0071519.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            24948..25202
                     /locus_tag="ACL297_001257"
     CDS             24948..25202
                     /locus_tag="ACL297_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HGR0071520.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
     gene            25567..25866
                     /locus_tag="ACL297_001258"
                     /pseudo
     CDS             25567..25866
                     /locus_tag="ACL297_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         8175 a         4576 c         5756 g         7440 t
ORIGIN      
        1 aacctgtggc tatcttccta gtttactttt tgatttttat tgagtataag aggtctttcc
       61 taagcagtta gaggagaatt ttgctatact gatactagta agtggtaaag gagcagagca
      121 tgacctacac aatcttaatc gtagaagatg aatatctggt aagacaaggt ttgactaaac
      181 tggtcaatgt agcagcctac gatatggaaa tcatcggtca ggctgaaaat ggaaggcagg
      241 cttgggaatt gatccaaaag caggtgccag atatcatttt aaccgatatc aacatgcctc
      301 atctaaatgg catccagttg gccagtctgg tacgagaaac ctatcctcag gttcatttgg
      361 tctttttaac aggttacgat gattttgatt atgccttgtc tgctgtcaaa ctaggtgtgg
      421 acgactacct gctcaaaccc ttttctcgtc aggatattga ggaaatgttg gggaaaatca
      481 aacaaaaact agacaaggaa gagaaagaag agcagttaca agatttattg actaataggt
      541 ttgaaggaaa catggcccag aaaatccagt ctcatctggc tgatagccaa tttagtttaa
      601 agtctttagc cagtgactta ggttttagtc cgacctatct gagttccttg attaagaaag
      661 agttgggctt gccttttcag gattatctgg tgagagaacg tgttaaacaa gccaagctct
      721 tgcttttaac tacagatctg aagatttatg agatcgcaga gaaggttggt tttgaagata
      781 tgaactattt tacccaacgt tttaagcaga ttgcaggtgt gacacctcgt cagtttaaga
      841 agggagaaga ccgatgaagc gttcttctct tttagttaga atggttattt ccatctttct
      901 ggtctttctc attctcctag ctctggttgg aactttctac tatcaatcaa gttcttcagc
      961 cattgaggcc accattgagg gcaacagcca aacgaccatc agccagacta gccactttat
     1021 tcagtcttat atcaaaaaac tagaaaccac ctcgactggt ttgacccagc agacggatgt
     1081 tctggcctat gctgagaatc ccagtcaaga caaggtcgag ggaatccgag atttgttttt
     1141 gaccatcttg aagtcagata aggacttgaa aactgttgtg ctggtgacca aatctggtca
     1201 ggtcatttct acagatgaca gtgtgcagat gaaaacttcc tctgatatga tggctgagga
     1261 ttggtaccaa aaggccattc atcagggagc tatgcctgtt ttgactccag ctcgtaaatc
     1321 agatagtcag tgggtcattt ctgtcactca agaacttgtt gatgcaaagg gagccaatct
     1381 tggtgtgctt cgtttggata tttcttatga aactctggaa gcctatctca atcaactcca
     1441 gttggggcag cagggctttg ccttcattat caatgaaaac catgaatttg tctaccatcc
     1501 tcaacacaca gtttatagtt cgtctagcaa aatggaggct atgaaaccct acatcgatac
     1561 aggtcagggt tatactcctg gtcacaaatc ctacgtcagt caagagaaga ttgcaggaac
     1621 tgattggacg gtgcttggcg tgtcatcatt ggaaaagtta gaccaggttc ggagtcagct
     1681 cttgtggacc ttgcttgggg ccagtgtcac atctcttctt gtctgtctct gcttagtgtg
     1741 gttcagtctt aaacgctgga ttgctccttt gaaggatttg agagaaacca tgttggaaat
     1801 tgcttctggt gctcaaaatc ttcgtgccaa ggaagttggt gcctatgaac tgagagaagt
     1861 aactcgccaa tttaatgcta tgttggatca gattgatcag ttgatggtag ctattcgtag
     1921 ccaggaagaa acgacccgtc agtaccaact tcaagccctt tcgagccaga ttaatccaca
     1981 tttcctctat aacactttgg acaccatcat ctggatggct gaatttcatg atagtcagcg
     2041 agtggtgcag gtgaccaagt ccttggcaac ctatttccgc ttggcgctca atcaaggcaa
     2101 ggacttgatt tgtctctctg acgaaatcaa tcatgtccgc cagtatctct ttatccagaa
     2161 acaacgctat ggagataagc tggaatacga aattaatgaa aatgttgcct ttgataattt
     2221 agtcttaccc aagctggtcc tacaacccct tgtagaaaat gctctttacc atggcattaa
     2281 ggaaaaggaa ggtcagggcc atattaaact ttctgtccag aaacaggatt cgggattggt
     2341 catccgtatt gaggatgatg gcgttggctt ccaagatgct ggtgatagta gtcaaagtca
     2401 actcaaacgt gggggagttg gtcttcaaaa tgtcgatcaa cggctcaaac ttcattttgg
     2461 agccaattac catatgaaga ttgattctag accccaaaaa gggacgaaag ttgaaatata
     2521 tataaataga atagaaacta gctaactccc agtcaattct gggagtttta tgcgtaattg
     2581 ggcaaacttt ctaggttgtc tatcttgcta aaaacaagaa aaaacggtat gatagaagag
     2641 tgacaacaga ggtaacagag atgaacgaga cacagattca aagagaaaca cgtcaggtag
     2701 tggaagatgt tttagaaaag accaatttga agcagggggc cctttttgtt ttaggtctat
     2761 cttctagtga ggtgctaggt ggtcatattg ggaaagaatc cagtcaagag attggtgagc
     2821 tgatagtgga gactatcttg gatattctag gtagtagagg tatccattta gcagttcaag
     2881 gttgtgagca tgtcaatcgg gctctcgttg ttgaacgtca ggtggcagag cagtttggtc
     2941 tggaaattgt cagtgtccat cctactcttc atgcaggagg ttcggggcag ttggcagcct
     3001 ttaagtttat gcaggatcca gttgaggttg aatttatcaa ggctcatgct ggattggata
     3061 tcggagacac tgcaattggc atgcatgtca agcatgttca ggttccgatt cgccctattt
     3121 tgagagagat tggtcatgcc catgtaacgg cactggctag tcgtccaaaa ttaatcggag
     3181 gtgcgcgtgc gcactatccg caagatgcta ttagaaagac ctgagaatga ataaaggaga
     3241 taaaagtgtt taaaaaagac cgtttttcaa ttcgtaagat taagggagtt gtaggctctg
     3301 tatttcttgg aagccttttg atggctcctt ctgtagtgga cgcagccacc tatcactatg
     3361 taaataaaga gattatttca caagaagcta aagatttaat tcagacagga aagcctaaca
     3421 ggaatgaagt tgtatatggt ttggtgtatc aaaaagatca gttgcctcaa acagggacag
     3481 aagcatctgt tttgacagct tttggttatc tatcaggtga catcctcaaa acgcttggct
     3541 tggacactgt tttagaagaa acctcagcaa aacctggaga ggtgactgta gtcgaagttg
     3601 agactcctca atcaacaaca aatcaggagc aagctaggac agaaaaccaa gtagtagaga
     3661 cagaggaagc tctaaaagaa gaagcaccta aaacagaaga aagtccaaag gaagaaccaa
     3721 aatcggagat aaaacctact gacgacaccc ttcctaaagt agaagagggg aaagaagatt
     3781 cagcagaacc agctccagtt gaagaagtag gtggagaagt tgagtcaaaa ccagaggaaa
     3841 aagtagcagt taagccagaa agtcaaccat cagacaaacc aacagaggaa ccaaaagttg
     3901 aacaagtagg tgaaccagtc gagccaagtg aagacgaaca ggcaccaacg gcaccagttg
     3961 agccagaaaa gcaaccagaa gctcctgaag aagagaaggc tgtagaggaa acaccgaaac
     4021 cagaagataa aataaagggt attggtacta aagaaccagt tgataaaagt gagttaaata
     4081 atcaaattga taaagctagt tcagtttctc ctactgatta ttctacagca agttacaatg
     4141 atcttggacc tgttttagaa actgcaaaag gtgtctatgc ttcagagcct gtaaaacagc
     4201 ctgaggtaaa tagtgagaca aataaactta aaacggctat tgacgctcta aacgttgata
     4261 aaactgaatt aaacaatacg attgcagatg caaaaacaaa ggtaaaagaa cattacagtg
     4321 atagaagttg gcaaaacctc caaactgggg ttacagaagc tgaaaaagtt gcagctaata
     4381 cagatgctaa acaaagtgaa gtaaattctg agactgcaag tcttaaaaca gcaatttcta
     4441 gattaaatac agataaagtt gaactcgaaa atcaacttaa gattgcacag ggaaaaactg
     4501 aaactgattt cagcatggaa tcttggactg ttttgagtac agctaaaaat aaagctcagg
     4561 aagtaaaaga taacggaaca gcgacccaag aacaaataaa cgaagcagag aaaagtttga
     4621 aaacagccct tgctgactta tctgttgaca aaacagcttt gggaagcgct atcgatacag
     4681 ctactaagaa aaataaagag aattatacaa atcaaacatg ggcagagttg gaaacagcgc
     4741 ttacagcagc aaaatctgta aatactaacg agtctaaaca aagcgacgtt aacgaagctg
     4801 ctgaaaaatt aactgcaact atggaaaaat tggttgaatt gtctgaaaag ccacgtttga
     4861 ctctatctat tgaaaagaga gacatagatc gaaaagctac ggtcacgtat actttggaaa
     4921 atccagcaaa tactcaaata aaatcaataa ctgcaactct gaaaaaagga gaggaagttg
     4981 ttaaggattt tgttttaaca gaagaaaatc ttaaaactaa tcatttaact gctttgtttg
     5041 aaaagctaga ctactacaaa gaatacactc tatctacgga tatggtatat aacagaggaa
     5101 atgatgatga gacggagagt atttcagaag aactaattca gctgaatctt aagaaactag
     5161 aattgaaaga tatccagaca gtctctctga tgaaatttga aaatggtcaa gaaagtcagg
     5221 ttacacactt gtctgataaa ccgaccgatt tgagtaaatt atacttaaaa gttacatcat
     5281 caacgtctaa ggatgctgtg ttagccgtta gctctataga agaagagatt attgaaaata
     5341 aaaaaatctt taaaattcat gcagatacac cagaattagt agttcgtaag aaagatggca
     5401 gtctttctaa gggatttgac tattacatgg agagagttat ccctcatgat ggtgatattt
     5461 attatgattt taaagacttg atttcagcta tgacatctaa tcctacaggt acgtttattt
     5521 tagggagaga tatttcctcg cgtaatgtaa aaccggatgg aaatggaaaa tcttatatca
     5581 aaggtgaatt taaaggaaaa ctacttggta caaatgataa tgttagacac tcgatctttg
     5641 acttagaata tccattgttt gatactataa aatcaggagt cgtgaaagat attgatttca
     5701 agcatgtcaa tatggtattc ccagatagca atcaaggaga taacgtagca acaattgcaa
     5761 gagtaatcaa agataagaca aaaattgaga atgttaatgt tgaaggttat ctcgagggtc
     5821 gagatcatgt agctggttta gttaataatc ttgaaggtaa ttccgaaatt gaaaatgtat
     5881 cctttacagg aaagattaag tctaaaggtg gtaattctat tacagcaggt attgcaggta
     5941 gaaatatctt atctcgtgtt aaacgtgcct atgtaaatgc agatatagag gtacatcgat
     6001 catctaattc tagcatgctt gttgcagtaa atggaataaa cgcagatgca agtggtggtt
     6061 ggggaacctg gggtagactt actgagtcag ttgctaaagg aacattagaa accaagcaag
     6121 gagggcaagc tggaggagcc tcttctacag tctggcctta tggagcaatt gataatgttg
     6181 tcagctatgc aaaagtgacc aaagggaaag aattatttgg atcagatggt gatttaaatt
     6241 atgattggtt tatgaaaaaa attagtaata tttttggtgt tcaaggcatc agctcaggcg
     6301 attcgggtag tgattctaaa tttacgcgta tttctgagga agaagcaaat caaaaagttg
     6361 cttcttataa tattacagct cccaacttga tgagtgactc aagtcttttg gtagacagac
     6421 tcaatgaaag ttggaaaaat actgatcaat ttgaaagtat acaggattat caagcccaaa
     6481 accaattaat ttaccagaac ttgactaaat ttactcctta ttacaataaa gaatttattg
     6541 ttcatgaagg aaatgctctc actcctgaac aggaaatatt gaagactaag aaaatcaaat
     6601 cgattgtagg gttgaaagga acagagttcg ttgtagatgg cagtgatatt gatactatca
     6661 tgcttcattt tgaggatggt agtcagaagc gatataaggt aacctctact ggtaagtttt
     6721 cgataactaa tttaccggaa tatcaggttg aagatttaaa tgtggtgtat acatcagaac
     6781 atatagtaca tccactagat tcgtcactga taaataacct tgttgaagaa ttgaaaaaag
     6841 tagaattgta tacagagtct acttaccaag ttctaggtat tgataaagat aatgctaaca
     6901 aattgaatag aactaagaga ttattcttag atgagtcatt agatgctgtt aaaacacaat
     6961 tgcctacctt tgtaaaaaca atgtttgaaa atgagtggtt gcacatcaat ggagaatcta
     7021 gtggagctgt cgctgcttta cgacaaaaaa tcatggataa taagacggct atattgcttg
     7081 ctttgactta tatcaatcgt tattatgatg ttaaattctc agactataat attaaaaaac
     7141 tgatgctctt taaaccaact ttccatggtg aaaagattga tctattagat cgattaatcc
     7201 gactaggatc atctggagaa aatagattaa aaggttcaga aaatgcagaa acgttcaagc
     7261 agttatttgc tagcgaaacg aagcagaagg atttggtgac ttatttagac tacaatcgct
     7321 ctttgttgac taattatcaa acaacagggg aatggtttaa agaaacgact aaggattata
     7381 ttcaatttga ggaacgtcca tccttagtag aggagattaa agatgccaaa tatcgagttt
     7441 acgataattt aaccgcaccg tattatcaag gatatatcct acctctctta acgttgaaga
     7501 atactcattt agcaatttta tcgaactata gtactatgac atttgtcagc cgtgaaaaac
     7561 gccctaattg gaaaaatgaa gattttgata agtgggtgaa atatgtagca actgctcata
     7621 gaaaccatgt tgatacttgg tataagattt tacctgataa tatcaaaggt aaaatggtta
     7681 aagagaatgt tactgctgtt tgggaaggat tgagcatccc aggaagtgaa tgggtagacc
     7741 agaatgctgt agatagaaaa ggaagagatt acgccccagc tcgagaattc tttaatcttg
     7801 taggtggtcc tatgggaggt tggtatgctt accatggtta tggtgcgcat gctggaggaa
     7861 gaaatcgggt taattatgag gtgtttgatg tattaagtga gtatggtata tcggtcttta
     7921 ctcatgaatt gactcacgtt aatgatactt ggatttactt aggagggtat ggtagacgtg
     7981 aaaatatggg acctgaagct tatgctcagg gattattcca gtctcctgtt ccaggtcagc
     8041 cgggttgggg ggcgcttggt ctcaatatgg catttgaaag aaaaaatgat ggtgatctaa
     8101 tttataatgc ctccccaact caatttgaaa ataggaaaga gttagatagt tatatgaaaa
     8161 attataacga tactctcatg atggtggact acttggaagg agatgcagtt atctctaaag
     8221 gtaaagaagc tattactaaa tggtttaaaa aagttgaacc taaggttgtt agtcaaacag
     8281 ctcagtatga tacggttcgt cagttgactg ctgaagagaa agaaaaactt tctgtttcta
     8341 gtgttgatga tttagttgat caaggattaa tgtcagacag agctgtagga aataatacct
     8401 ataatccagc tgattttgaa acaagttata ttgcgataga ttatatgact ggtatttatg
     8461 gtggaggaaa gaatagtgta ggttcaccag gtgccttgat gtttaaacat aatactttcc
     8521 gtatgtgggg ctactatggt tttgaagaag gtgttctagg atatgcttct aataaattta
     8581 agcaagcatc tagagatgaa ggtcatgctg gacttagtga taactttatt atttctaaga
     8641 tttccaaagg ggaattttta actatggaag catttaaaaa gggatatttt aagaaagtag
     8701 tagaagagtt aaaaactaaa ggtattcgtc ctgttactat caatcaaaaa acatatagca
     8761 cctttgaaga attgcaagaa ggatttaagc aggctgttga aagagatttg aagaaaaatc
     8821 agttagatga gagggaaaca agaaacttca aattccaagt tttcagacaa cttcttcaac
     8881 aaacagatag ctttaaaact tctatcttta gataatcgaa atctctcatc tgctttgcgg
     8941 atgagggttt tcttatttta tgctatactt aaagtatgca tagaaaaaca gtgattgatt
     9001 ttaggacttt gggggagaga tataccttta cccagcctat taaagagttg aaaacgagaa
     9061 atgtagaaga agtggcagat ttgctggcac aagtggaaag ctaccaagag caaggttatt
     9121 atgtggtggg gtatgtcagc tacgaggctg cacctgcttt tgaggaaaaa ttagcagttc
     9181 acaaggatcc cctactggga gagtacctgc tttattttac tgttcatgat agcgtagaaa
     9241 cttcccctat tcctctgact tatgaggata ttgatttgcc ctcaaattgg caggaagtaa
     9301 cgtctgcagc agactatgaa aaggccattg cccaaattca ccatcattta cggcagggag
     9361 atacctatca ggtcaactac accgtccaac ttaagcaaaa gttaaatgcc aatccttttg
     9421 ccatctacaa tcgtatggtg gtagagcagg aggcgggcta caatgcctat gtggaacatg
     9481 acgagatggc agtgatttcc atgagcccag agctcttttt tgagcaaaat gatcgcgagt
     9541 tgacaacacg accaatgaag gggacgactc agcgtggggt aactgaccaa gaagatcttg
     9601 aacaggccag ttggttggaa caggatccta aaaatcgctc tgaaaatatg atgattgtgg
     9661 acctcttgcg caatgatatg aaccgtattt ctgaagttgg gagcgagcac gtggagcgtc
     9721 tgtgtcaggt agagcagtat tcaactgttt ggcagatgac ttcgaccatc aagagtcagt
     9781 tgcgagagga tgtggacctt gttgaaatct tccactcact ctttccttgt ggttccataa
     9841 cgggtgcacc gaaaattgcg acaatggaga ttatcaagga cttggagcct caaccgcgtg
     9901 gagtctactg tggaacgatt ggtctcttgc ttccaaatgg acgacggatt tttaatgtgg
     9961 ccattcgtac cattcaactt cacaaaggtc aagccattta tggagttggc ggagggatta
    10021 cttgggatag cacttgggaa tctgaatacc gtgaagttca tcaaaaggca gcagttctct
    10081 atcgtaaaca agctcgcttc caattaatta caactggaaa aatcagccaa aaacaactga
    10141 tgtttgaaga acaacatctg gaaaggctga gaaaagctag tcggtatttt gcttatcctt
    10201 ttgacccaga aattttgaga caaaagattg aagcagagtg tcagacttgt gatgctaatc
    10261 aagattaccg cttgcgaatt tctctcagca agtctggaga gatagaattc agtcgtcaaa
    10321 tcttaacacc tcttagtcca agtttttgtc aggccaaact ttgtctgcaa gaagcgaatt
    10381 tgaatcaatc ctttacctat tttaaaacga cttacagacc gcatttgagc atagaggaac
    10441 aagagaagat ttaccataat ggaaaaggag aacttcttga aacctctatc ggaaatttgg
    10501 ttctgaaaat cgatgggaaa ctctacacac cgcctatccg acttggaatc ttgccaggaa
    10561 tttatcacca gtatttacta gaaacaggac aggtagaaga gaaagtcttg accttggcag
    10621 attttgccca agcagaagct atttacggct gtaatgcagt gagaggcttg tatgagttgc
    10681 aagtgatagg attgtgagtt ttagctatta atagttttta atatatatta aaatatattg
    10741 gtgtttactt ttgatgagat atcatatttg tgttataatg taaccgtaaa caatagaaat
    10801 ttaaaggaga tttttgatga aatcaaaaat agttttggga gcttcgctag cgatcgcaac
    10861 acttagctta gtatctcttg tcgagattga aggactctca ccgtttttaa tagagaatgt
    10921 tagcgcaaat actcattctg caaataaagt tattaatcat aaagtaagta tttacttaga
    10981 aaatgctgat gaaggtaaag gtctaactgt aaacttttca acagacagtg ttagtccaaa
    11041 tttatttgat gagttcgaaa aaaagagtgg aattacaata actactatgt tagtgaatgc
    11101 taagacaggt gaagttgtag aaaaaagatt aacaccaagc gtttttttac gttccaatga
    11161 tttaaccagt gggaccatat cttcgtttat ttttagtgaa tatcctgatg gagaatataa
    11221 atatgttgtg tctaaaggag actttatcga tcctaagact cagtttaaac atcagtatag
    11281 aggagaatct ccagttttta gaatacgtaa tcgaaaatat gtggagttgg gaacaactga
    11341 taaaaaatta gatgaaagac gtgataatag cgtttataaa gatggggttg ttgaacacaa
    11401 ggtaaattta agtttaacaa gttatcaagg aggtaatgga gttactgcaa ttttttcaac
    11461 tgatagtgtt aactctaact tacttaattc ttttggtgaa aaggctaaaa aagttttgat
    11521 taggtctaag ttaattaatg ttaagactgg cgaggttatt gatgaaacgt tttctccaaa
    11581 agtaagttta acctctaaaa ttttgaaaag tggttctaca gcagtatttt attttataga
    11641 tttaactgat ggcgaatata aatatgttgc gtatgaaagc cagcaatata ctgatcctca
    11701 aacaacattg actcatcagt atagaggaga atctcctatt tttagtatca aagatggaaa
    11761 attttctggt ttagtatctg cctcaaaacc agacgaaaac ccgaagccaa cgccaaaacc
    11821 ggatgagaaa ccaaaacctt cagcaccaca acaggagaaa acaaaaccta cagttcagtc
    11881 tggttgggta ggttcatctt attatcaaaa tggtaaaaaa gtcactagta agtggatttt
    11941 tgataaaaaa tataattcat acttctatct agatgcatca ggaaattatg ttcaaaacgc
    12001 ttgggtaggt aactattatc tcaaatcagg cggatacatg gctaaaagtg agtggattta
    12061 tgataagaac tatggttcat actactatct aacatcagaa ggcagctacg ctcgtaacac
    12121 ttggtcaggc aattattatt tgaagtcaaa tggtaagatg gctaaaagtg agtgggtata
    12181 cgatagtaac tacaaatcat attactactt gacatcagaa ggaagttatg ctcgtaacac
    12241 ttgggtaggc aattactatc ttaaatcaaa cggtaaaatg gctgttaatg aacgtacacc
    12301 agatggttat cgtgtagatg gttcaggtaa atgggttaaa taagaaacta aaaaatagga
    12361 taataggtaa tatcagatga ataaaaaaaa ttattttcat gtgctttagc attttcaacg
    12421 attggaacat tgtcaacgat tgataaatca ggagttcttc ctttttcagc gcaaacggtg
    12481 caggctgaca tacaaacgct taccccaatg gcaaaatatt atattattct tccgaaggat
    12541 gcagaaatat ataaaacttg gagaggtact gtcaatattc ccattattga tgctacaaaa
    12601 actactccag aattatctta tttcaaagaa gatcatcgca actatatagc taatgagaat
    12661 aagtctggtg ccaattacat agagtggaag ggaacagtag aagagtttaa agaggccatt
    12721 aagaagttga ctgataaaaa atcaactact gcaacaccaa aaaaggatga aaagccaaca
    12781 ccaaaaccgg atgagaagcc aaaaccaaca cctacagttc agtctggttg ggtaggttca
    12841 tcctattatc aagatggtaa aaaagtcatt agtaagtgga tttttgataa aaaatataat
    12901 tcatacttct atctagatgc atcaggaaat tatgttcaaa acgcttgggt aggtaactat
    12961 tatcttaaat caggcggata catggctaaa ggtgaatggg tttatgatgc cacctatcaa
    13021 gcttggtatt atttgacatc agatggttct tatgcttaca gtacatggca aggaaattac
    13081 tatctaaaat cggatggtaa aatggctgtc aatgaatggg ttgatggtgg acgttattat
    13141 gttggcgctg acggagtttg gaaggaaggt caagcaagta cagcttcttc tagtaatgat
    13201 agcaatagtg aatattctgc tgctttagga aaggcaaaaa gttataattc gttattccac
    13261 atgtcaaaaa aaaacgtatg tatagacaat taacttctga ttttgataaa ttttcaaatg
    13321 atgcagctca atatgccatt gatcattaga tgattaacag atactggtag gcgaaaaaat
    13381 tccttataat tcatttacgc aaacctttgc ataagtgaat ggattttgat atactagtac
    13441 tgtaagcatt ttcaattact acacaatgaa aaaggagaat atgatgagtc aaaagattat
    13501 tgggattgac cttggtggaa cttctatcaa atttgcaatc ttaacaacag caggagaaat
    13561 ccaaggaaaa tggtcaatca agaccaacat tttggatgag ggaagtcata tcgttgatga
    13621 tatgattgag tctattcaac atcgtttgga cttgcttgga ttggcagcag cggacttcca
    13681 aggcattgga atgggatcac caggtgtggt tgaccgtgac aaagggactg ttatcggtgc
    13741 ctacaacttg aactggaaaa cccttcaacc aatcaaacaa aagattgaaa aagctttggg
    13801 cattccattt ttcatcgata atgatgccaa cgtagcagct cttggtgagc gctggatggg
    13861 tgctggagat aaccaaccag acgttgtctt tatgacactc ggtactggtg ttggtggcgg
    13921 tatcgtcgca gaaggcaaat tgcttcacgg tgttgctggt gcagcaggtg agcttggtca
    13981 catcactgtt gactttgacc agccaatctc atgtacttgc ggtaagaaag gctgccttga
    14041 gacagttgct tcagcaacag ggattgtcaa cttgactcgt cgctatgccg atgaatacga
    14101 aggcgatgca gccttgaaac gcttgattga taatggagaa gaagtaactg ctaagactgt
    14161 ctttgatctc gcaaaagaag gagacgacct tgctttgatt gtttaccgta acttctcacg
    14221 ttacttggga atcgcttgtg ctaacattgg ttcaatccta aacccatcaa caatcgtcat
    14281 cggtggtggt gtgtcagctg cgggagaatt ccttctacaa ggtgttcaaa aagtttacga
    14341 tgaaaatagt ttcccacaag tacgcacatc tactaaatta gctcttgcaa ctctaggaaa
    14401 tgacgctgga gttatcggag cagcatcact tgtattgcaa taagataatg agagaggaaa
    14461 atgctaacct agagttagta atgttcctct tttatttttt atgctatact agttaggaca
    14521 atgaatgagg tgagagtaaa tgacaaaagc agatacgatt tttaaagaga atattgaatg
    14581 cattctaaag gaaggtgttt tctcagagca agctcgtcct aagtacaagg atgggactgt
    14641 tgctaattcc aagtatgtaa ctggtgcctt ttccgagtac gacttatcta aaggagaatt
    14701 tcctatcaca accttgcgtc ccattgcaat caaatccgcc atcaaagaag ttctctggat
    14761 ttaccaagac cagtcaaata gcttagaagt gcttaatgac aagtacaatg ttcactactg
    14821 gaatgactgg gaagttggag acacgggaac cattggtgag cgctatggtg ccgttgttaa
    14881 gaaacacgac attatcaata agcttctcaa acagttggaa accaatcctt ggaatcgccg
    14941 caatattatt tcgctctggg attaccaagc tttcgaagaa acagatgggc tgctcccgtg
    15001 cgcctttcag accatgtttg atgtccggcg tgttgatggg gaaatctatc tggatgcgac
    15061 cttgacccag cgctccaatg atatgctggt ggcccaccac atcaacgcta tgcagtatgt
    15121 ggctttacag atgatgattg ccaaacattt tggttggaag gttgggaagt tcttctattt
    15181 catcaacaac ctccatatct atgataatca atttgaacaa gctcaggaat tgctccgtcg
    15241 ggagccgtca aactgccaac cacgcttggt tttaaatgtt cctgatggga ctaatttctt
    15301 tgatatcaaa gcagaagatt ttgagttggt ggattatgac cctgttaagc cacagttgaa
    15361 gtttgaccta gctatttaaa agaatagaaa aaagaagttg agaataatcc caacttcttt
    15421 tgtttcttaa cgtgatacgc ggcgacgagc tgctttttta cgattttctt cgatgaaagc
    15481 tgctttttgc tcttctggtt cgattacttt ctttttaaat gcgtatactg cacctgcaac
    15541 ggcagcgaca gttcctgcga cacctgttac aagaccttta gcgaatcctt tagccatgag
    15601 tcttcctcct ttatattctc aatcagccag cctcctcaag aggtcacatt tttctgactg
    15661 acctttttgt gttataataa tagtaacgaa aaaatgggaa tttttcaagg aaaaaagatg
    15721 aaaacaaaaa taattgtgat tgttggaccg actgctgttg gaaagacggc tctagctatt
    15781 gaagttgcaa agcgctttaa tggcgaagtg gttagtggag atagccagca agtctatcga
    15841 ggacttgata ttgggacggc caaggctagt ccagaagagc aggcagctgt tcctcatcat
    15901 ttaatcgatg ttagagagat aaccgagtct tactcggctt ttgattttgt ttcagaagct
    15961 aagatgacta ttgaggatat tcagagccgt ggcaagctag ccattatcgc cggtgggact
    16021 ggactttata tccagagctt gctagaaggt taccacctag gtggggagac tcctcatgag
    16081 gagattttag cttatcgagc tagtttggag ccatattcag atgaggaatt agcccatttg
    16141 gtggagcaag caggccttga gattccccag tttaatcgtc gtcgtgctat gcgtgcctta
    16201 gaaattgccc attttggtca ggatttggaa aatcaagaga tcttgtatga accgctgatt
    16261 atctgcttgg atgatgaacg tagtcaactt tatgagcgta tcaaccaccg agtggatttg
    16321 atgtttgagg ctgggctttt ggatgaggcc aagtggctgt ttgaccattc ccctaatgta
    16381 caggctgcta aaggaattgg ctataaggaa ctctttcctt atttccgtgg agagcagacc
    16441 tttgaggaag ctcgtgagag tcttaaacag gcgacccgtc gttttgccaa gcgtcagctg
    16501 acctggttcc gtaatcgcat gcaggtcacc ttttatcaga ttggagaatc tggtgtacaa
    16561 gaccgcattt taagccagat agaggagttt ttagatgatt gaaacggaga aaaaagagga
    16621 gcgagtcctg ctgattggtg tggaattgca gggtatggac agttttgacc tctccatgga
    16681 agaattggct agtttagcga aaacggcagg ggcagtcgtt gtagatagct acagacaaaa
    16741 acgtgaaaaa tatgattcca agaccttcgt cggctctggt aagttggaag agattgcgct
    16801 tatggtggat gcagaagaaa tcactactgt catcgtcaac aatcgtctga ccccaaggca
    16861 gaatgtcaat ctagaggaag ttctcggtgt taaggtcatt gaccgtatgc agttgatttt
    16921 ggatatcttt gccatgcggg ctcgaagcca tgaagggaag ctccaagtcc acctagccca
    16981 actcaaatac ctcttgcctc gcttggttgg tcaggggatt atgctcagcc gtcaggcagg
    17041 gggaattggt tcccgtggtc ctggtgaaag ccaactggag ctgaaccgtc gtagcgttcg
    17101 caatcaaatc acggatatcg agcgccagct taaggtggtt gagaaaaatc gtgcgactgt
    17161 cagagaaaaa cgtttggagt ctagcacttt taagattggt ttgattggtt atactaatgc
    17221 tgggaaatca actatcatga acatcttgac cagtaagacc cagtatgaag cagatgagct
    17281 ctttgcgact ctggatgcga caaccaagag tattcatctg ggaggcaatc tccaagtaac
    17341 tttgacagat accgttggct ttatccaaga tttgccgaca gagttggtgt ccagtttcaa
    17401 gtcaaccttg gaagaaagca agcatgtgga ccttctggtt catgttatcg atgctagcaa
    17461 tccttaccac gaggagcatg aaaaaacggt tctctccatc atgaaagacc tggacatgga
    17521 agatattcct cacttgacgc tttataataa agcggatttg gtggaggatt tcacgcctac
    17581 ccaaacgcca tataccctca tttctgccaa gtctgaggac agtcgtgaaa acttgcaagc
    17641 attattgcta gataagatta aggaaatttt tgaagcattt accctgcgag tgcctttttc
    17701 aaagtcctac aagattcatg atttagagag tgttgcaatt ctggaagaac gtgattatca
    17761 ggaagacggc gaagtgatta caggctacat ttccgagaaa aataaatgga ggttagaaga
    17821 attttatgac tgatattaaa actttggctc taaagtatgg aggttataca agtctggaca
    17881 aggtctatct ggatcagctt ctagctggta aaacagatca ggagcagtta acacttatta
    17941 cacctccgcc aagtgttgtt aatgcctact ttgcagaact ctatcagaaa aagagtcctc
    18001 aggctgcgac ggattatttt gcagaactca gtcaggaact gaatctttac aatactgagc
    18061 caagtttcac cctagaaaat aagcctttta ttcggcttaa tctgtctggt aaatcctttg
    18121 gtttttgtta tgagagtgac ggccttggtc ggattttctt tgaaaatgag gagaaaatct
    18181 cggatgactt gctctttgaa attgcgcaaa ttttccccca tcaattagtc tttgaagagt
    18241 ctggtaagat ttacatgaag cctgtcgagg acgaggaagt tatcagccta gaaagtctca
    18301 cagctttgac tgatttggaa agattggctg atggtcgaaa acgtctcaaa ggctacagcc
    18361 aagaggattt attgcaagaa gctgctgctt tttctggcaa acgctatttc cgatcggaaa
    18421 accgcacagc catgttatat attgattaat tagaaagtat cgaatggata ttcaattttt
    18481 aggaacgggg gctggtcagc cctctaaagc ccgcaacgtt tcaagtctcg ccctgaaact
    18541 tttggatgag attaacgaag tttggctctt tgactgtgga gaaggtacgc aaaatcgcat
    18601 tctggaaacc acaattcgac cacgtaaggt cagcaaaatc tttattaccc atctgcatgg
    18661 agaccacatt tttggtttgc caggtttcct ttctagccgt gcctttcagg ccaatgaaga
    18721 gcagacagat ttggaaatct acggacctca aggaatcaag tcatttgtct taaccagcct
    18781 tcgtgtgtca ggttctcgtc tgccctaccg cattcatttc catgagtttg accaagattc
    18841 tctgggtaaa attcttgaaa ccgataaatt cactgtgtat gcagaggagc tggaccacac
    18901 tattttctgt gttggctatc gtgtcatgca aaaggatcta gaagggacgc tggatgctga
    18961 aaaactcaag gctgctggtg ttccgttcgg cccgcttttt ggtaaaatca aaaacggcca
    19021 ggatcttgtt ttggaagacg gaactgaaat caaggcagca gactatatct cagcgccacg
    19081 tccaggtaag attatcacta ttttaggaga cactcgaaaa acgggtgcca gtgtgcgtct
    19141 ggctgtcaat gcagatgtcc tagttcatga gtccacttat ggcaagggtg atgaaaaaat
    19201 tgctcgtaac catggtcact caactaatat gcaagctgca caagtagcgg tagaagcagg
    19261 tgccaaacgc ctcctactca accatatcag tgcccgtttc ctctcaaaag atattagcaa
    19321 actcaagaaa gacgctgcca caatttttga aaatgtccat gtggtcaaag acttggaaga
    19381 agtggaaatc tagcagtcac agaaaggata agtatgccta ctattctcat taccggagct
    19441 agcggtggtc tagctcaaga aatggtcaaa ctcctgccca atgaccaact catcttgctt
    19501 ggtagaaata aggaaaaatt agcccaactc tacggaaatt attcccatgc agaattgatt
    19561 gaaattgata ttaccgacga ttcagcccta gaagctctgg taactgatct ttatctccgc
    19621 tatggcaaga ttgatgtctt gattaacaac gctggttacg ggatttttga gggatttgac
    19681 cagattgctg ataaagatat tcaccagatg tttgaggtca atacctttgc cctgatgaat
    19741 ctgtctcgtc accttgcggc tcgtatgaag gaaagcagca aagggcatat catcaacatc
    19801 gtcagcatgg caggtctaat agctactggc aagtctagtc tttactcagc gaccaagttt
    19861 gcggctattg gtttttcaaa tgctctgcga ctcgaactta tgccctatgg agtctatgtg
    19921 acaacagtca atccaggtcc aatccgaaca ggatttttcg accaagccga cccagatgga
    19981 acttatctta aatcggttga ccgcttcctg ctagaaccag atgcagtggc taaaaagatt
    20041 gtcaagatta taggaaaaaa caaacgagaa ctcaatctcc cgattttgtt gaacctagct
    20101 cataagtttt ataccctctt tcctaagcta gctgataagt tggcagggga aacttttaat
    20161 tataagtaaa aagaaccaat gtgcaggttg ttgctagcct acatattggt tcttatcatt
    20221 tctcaactat caaactgaac ttcttctagg agatagtcta taaagcgttc gcccatctta
    20281 gataggctgg ttttctcatg ctggatatag accagctcga tcgggtcatc aatatccagt
    20341 gggatagaaa cgatattgtc tccgtttagg ttgctgttca aaatccctgt cgcaatggta
    20401 taaccatcca aaccaatcaa gagattaaag agggtggcac ggtcactgac cacaatggat
    20461 ttcttgtggt gttcttgaga aagaatctct tctgaaaagt agaaggagtt gtgcgtccct
    20521 tggtcatagc tgaggtaagg gaaattctcc aaatcagaca gtttcacctt gtctttcttt
    20581 gccagagggt tggtcttgct gacaaagata tgtggttgcg ctgtgaagag atggtgggct
    20641 agcaggtgat tgtcatccag catcttggtt aaaacatcac ggttgtaact gtttaagaag
    20701 aggaccccga cctcactgcg gaagttcttg acgtcgtcga taatctccca agtccgagtt
    20761 tcacgaagga agagttcgta tttctccata tcgcttttct tgagcaaaga gacaaaggca
    20821 ttgaccacaa aggcatagtg ttgagacgaa acgctaaata gttcgcggtg ggcgacagga
    20881 tttttatagc gttcctccag aagctgggtc tgctcgacaa cctgacgggc ataagagaga
    20941 aactccatcc catcacgggt caaggtgatt cccttgggat tgcggataaa gatctcaatg
    21001 cccatttcat tttccaaatc tcgcactgca ttggagagac ttggctgagt gataaagagt
    21061 tgcttggctg cctcattcat ggagccagtt tcgacgattt tgataatata atgtaattgt
    21121 tgaattctca tgtttttatt gtaccatact tgccttagaa tcgctaaaga agataggaaa
    21181 ataaggagca ttgtgtgagt ctcttcttga aaaaaacaca aaattttgct agaatgagtc
    21241 ttatgaaaac attctatgat gtgcagcaat tcctcaaacg atttggtatt attgtttaca
    21301 tgggaaaacg cttatatgat attgaactga tgaagttgga actatctcgg atttacgatg
    21361 cagggttgat ggataaatta gactatctag aggcagaagc agttcttcgc agagagcaca
    21421 aggtagaatt ggattatatt gagaaaaatg gagaaaagaa ctaatggtta cttggatttt
    21481 gtgggcactt atactagcaa tgttggcgtg gatgggcttt aactatcttc gtattcgccg
    21541 tgcggctaaa attgtggaca atgaggagtt tgaagccttg attcgtacgg gtcaattgat
    21601 tgatttgcgc gacccagcag aattccacag aaaacatatc cttggtgcac gcaatattcc
    21661 ttcaagtcag ttgaaaacta gtcttgcagc ccttcgtaaa gataaacctg tccttctcta
    21721 cgagaaccaa cgtgcgcaac gagttacaaa tgcagctctt tacttgaaaa aacaaggttt
    21781 ttctgagatt tatatccttt cttatggctt ggattcttgg aaagggaaag tgaagactag
    21841 ctaaccttat ttccaaatta gtcgaatgat aggatgaagc tattgtttta tagtataatt
    21901 aatttgtata aaatttaagg agttcttaga acataaaaga aaagaagaaa aatccacttt
    21961 ttgtgggtat cttgtcgatt attcttggtt tgttatttcc aattgtaggc ctgattttgg
    22021 gtatcatagg attggtcttg gctatttcat accaaaaaga gtcataatta gactataaaa
    22081 tagaaaagat tcttaatatc ttaggaattg tgatttctgt agttaactgg attgtagcaa
    22141 tcgccttaat ttttcggtaa ataaaagaaa aaagctttag ttttaaagct ttttttgttt
    22201 tatcgactca tctctaaata gtttttaatg atttgtaact ctttttggtt caaagggcgg
    22261 tagttacttt ctgctaaatc tgggttcaat gtgaagtccc caaattggat tctcttgagg
    22321 atagtcacct taacaccaac cgagaggaac attttcttga tttgatgaaa ttttccttct
    22381 gaaatggtga tagaggcttg actgatggag ggacttgcag atagaatctc tagttttgcg
    22441 ggtttacaga cagtaccatc taaaaagaca attccctttt gaaaggcttg gatatggtca
    22501 ggtgttagaa gtccattaac ctcaacttga taagtcttat cgacatgata ttggggatgg
    22561 aggagttgga agcccaaggg accgttatcg gtcaagagga ggagtcccgt tgtatctcgg
    22621 tccagtcggc caacggcata gagcttgtca gactggatgt cagatggaag caggtccatg
    22681 acggtcggaa gttccttgtc tttgttggct gtaacgacac cagcaggttt atgaagcata
    22741 agataggtgt gttcatagcc ttgaatgatt cgatcctgaa aaaggagttc ttgtagtcct
    22801 gtatcgatat tttgagctag ggagcgggct gggcaaccat cgactagaat tttccctctg
    22861 aggagtgctt gcttcatggc ctttcggctg attttttctt gggctaataa attatctaaa
    22921 cgcatactgt gcctatctta tcataagtcg cttgctttga aaaggcttga cgtgaaaaga
    22981 aaagcggagt gaaaacattt acacttgctt gagttatgtt atttatttga aattatggta
    23041 taatcgttca gttagaaaat aaattttgaa tattatagag gaaatcatga caaaattaag
    23101 agaagatatc cgtaacattg cgattatcgc ccacgttgac cacggtaaaa caaccctggt
    23161 tgacgaatta ttgaaacaat cagaaacgct tgatgcacgt actgaattgg cagagcgtgc
    23221 tatggactca aacgatatcg aaaaagagcg tggaatcacc atccttgcta aaaatactgc
    23281 cgttgcttac aacggaactc gtatcaacat tatggacaca ccaggacacg cggacttcgg
    23341 tggagaagtt gagcgtatca tgaaaatggt tgacggtgtt gtcttggtcg tagatgccta
    23401 tgaaggaacc atgccacaaa ctcgtttcgt attgaaaaaa gccttggaac aagaccttgt
    23461 cccaatcgtg gttgttaaca aaatcgataa gccatcagct cgtccagcag aagtagtgga
    23521 tgaagtcttg gaacttttca tcgagcttgg tgcagatgac gaccagcttg atttcccagt
    23581 ggtttatgct tcagcgatca acggaacttc ttcattgtca gatgatccag ctgaccaaga
    23641 agcgactatg gcaccaatct ttgacacgat tatcgaccat atcccagctc cagtagataa
    23701 ctcagatgag cctttgcagt tccaagtgtc acttttggac tacaatgact tcgttggacg
    23761 tatcggtatc ggtcgtgtct tccgtggtac agttaaggtt ggggaccaag ttaccctttc
    23821 taaacttgac ggtacaacta aaaacttccg tgttacaaaa ctcttcggtt tctttggttt
    23881 ggaacgtcgt gaaatccaag aagccaaagc gggtgacttg attgccgttt caggtatgga
    23941 agacatcttt gtcggtgaaa ccatcactcc gacagatgca gtagaagctc ttccaatcct
    24001 acacatcgat gagccaactc ttcaaatgac tttcttggtc aacaactcac catttgctgg
    24061 taaagaaggt aaatgggtaa cttctcgtaa ggtggaagaa cgcttgcagg cggaattgca
    24121 aacagacgtt tcccttcgtg ttgacccaac tgattcacca gataaatgga ctgtttcagg
    24181 acgtggagaa ttgcacttgt caatccttat cgaaacaatg cgtcgtgagg gctatgaact
    24241 tcaagtatct cgtccagaag ttatcgtaaa agaaatcgac ggtgttaaat gtgaaccatt
    24301 tgaacgtgta caaatcgaca ctccagaaga ataccaaggg tctgttatcc aaagcctttc
    24361 tgaacgtaaa ggtgaaatgt tggatatgat ttcaactggt aatggtcaaa ctcgtttggt
    24421 cttccttgtt ccagcgcgtg gtttgattgg atactcaact gagttcttgt caatgactcg
    24481 tggttacggt atcatgaacc ataccttcga ccaatacttg ccattgattc caggggaaat
    24541 tggtggacgt caccgtggtg cccttgtttc tatcgatgct ggtaaggcta caacttactc
    24601 aatcatgtct atcgaagaac gtggtacgat ctttgtcaac ccaggtactg aggtttatga
    24661 aggaatgatc atcggtgaaa actctcgtga aaatgacttg acagttaaca tcacgaaggc
    24721 aaaacaaatg accaacgttc gttcagctac taaggaccaa acagctgtta tcaagacgcc
    24781 tcgtatcttg acacttgaag agtctcttga gttcttgaac gacgatgagt acatggaagt
    24841 aacgcctgag tctatccgtt tgcgtaaaca aatccttaac aaagcagagc gtgagaaagc
    24901 taacaagaag aaaaaatcag ctgaataaga gctagaaaga gagaaagatg gtctatttag
    24961 tcctaggaat tttactgctc ctactctatg tatttgcgac accagaaagc attaaaggga
    25021 cagtcaatat cgtcgctatg gtatgtattt tagtggcact cttgatttta ttggttctat
    25081 cttttctgaa aatttttcaa ttaccaacag aaatattcct agcaatagcc atgttgctcc
    25141 tagcttactt tagtgttaga gacattacac tcatgccagt caaaaaaagt aaaagaagat
    25201 aaaaaagaga gttttggctc tcttttttgc taggaatttt acaagattgc aatttatttt
    25261 tgagttatca ccatctcttt tgtttggata atatgaatgg gaaaaacttg aataaaattc
    25321 gttaatggaa tctattttat atttggttag tactggttgc atattatttt gaaataaaac
    25381 tactatataa tattattcat agaggtaata tagtaaaata tctaaaaaat tcgatttttt
    25441 ccttgacaac aaataataaa aatgttattc ttatcacata agaaagcggt ttcaataaaa
    25501 gagagaggag gatttatttt gttgcattgt tgctcaaggt gtgatttttt tgaaagaaaa
    25561 aataaaatga aaacgaaaaa acatagatta cttgctctag ctcttatttc aagttttaca
    25621 ttattgggag ctgcatcagc tgctgtataa tatccagatg gaggagtatg gacatatgga
    25681 gaaggttcag gaggtggtta ggctttttca aattactatc atggtaaaaa atatcattat
    25741 tcatccatcg tgagcagatg ggatggtcat tctgataaag gagaagctcc tgctggaaaa
    25801 acctcctatg catggatttg gactaaatgg ggagaacaag tagcatttta ctgtgattat
    25861 gactaatcgt tcattgattt aaatcgttcc ccttgtaaga aattacaggg ggaattttgt
    25921 ttacaaggag tctaccaagg agtctac
//
LOCUS       DBEJAQ010000028        25588 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.028, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000028 DBEJAQ010000000
VERSION     DBEJAQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.028"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            69..566
                     /locus_tag="ACL297_001259"
     CDS             69..566
                     /locus_tag="ACL297_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HGR0071521.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            566..2221
                     /gene="dnaX"
                     /locus_tag="ACL297_001260"
     CDS             566..2221
                     /gene="dnaX"
                     /locus_tag="ACL297_001260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGR0071522.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2249..2443
                     /locus_tag="ACL297_001261"
     CDS             2249..2443
                     /locus_tag="ACL297_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HGR0071523.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2593..3363
                     /gene="sufC"
                     /locus_tag="ACL297_001262"
     CDS             2593..3363
                     /gene="sufC"
                     /locus_tag="ACL297_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGR0071524.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEGDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3391..4653
                     /gene="sufD"
                     /locus_tag="ACL297_001263"
     CDS             3391..4653
                     /gene="sufD"
                     /locus_tag="ACL297_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGR0071525.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4664..5890
                     /locus_tag="ACL297_001264"
     CDS             4664..5890
                     /locus_tag="ACL297_001264"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGR0071526.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5877..6317
                     /gene="sufU"
                     /locus_tag="ACL297_001265"
     CDS             5877..6317
                     /gene="sufU"
                     /locus_tag="ACL297_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HGR0071527.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6371..7783
                     /gene="sufB"
                     /locus_tag="ACL297_001266"
     CDS             6371..7783
                     /gene="sufB"
                     /locus_tag="ACL297_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGR0071528.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(7982..9223)
                     /gene="pbp3"
                     /locus_tag="ACL297_001267"
     CDS             complement(7982..9223)
                     /gene="pbp3"
                     /locus_tag="ACL297_001267"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HGR0071529.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9326..10435
                     /gene="cozE"
                     /locus_tag="ACL297_001268"
     CDS             9326..10435
                     /gene="cozE"
                     /locus_tag="ACL297_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozE"
                     /protein_id="HGR0071530.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLTAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11081..11428
                     /gene="ssrA"
                     /locus_tag="ACL297_001269"
     tmRNA           11081..11428
                     /gene="ssrA"
                     /locus_tag="ACL297_001269"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11611..11698)
                     /locus_tag="ACL297_001270"
     tRNA            complement(11611..11698)
                     /locus_tag="ACL297_001270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11662..11664),aa:Ser,seq:gga)
     gene            11861..12601
                     /locus_tag="ACL297_001271"
     CDS             11861..12601
                     /locus_tag="ACL297_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGR0071531.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12598..13509
                     /gene="pfkB"
                     /locus_tag="ACL297_001272"
     CDS             12598..13509
                     /gene="pfkB"
                     /locus_tag="ACL297_001272"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGR0071532.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13506..15458
                     /locus_tag="ACL297_001273"
     CDS             13506..15458
                     /locus_tag="ACL297_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGR0071533.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHSYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQVP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15595..17847
                     /locus_tag="ACL297_001274"
     CDS             15595..17847
                     /locus_tag="ACL297_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGR0071534.1"
                     /translation="MERKEAIQRMLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGS
                     LAYLAIFGLLIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGT
                     MAQVVTDLTGFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWS
                     VYDIAEFFSRGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPP
                     VDMETGEILTEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAK
                     EALEYKLPSLQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVT
                     KYEVKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFR
                     ELWEQSQTKAENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASI
                     LMKARPDQVKFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYEL
                     FAKVGVRNIAGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRL
                     GQKARAAGIHMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLL
                     GRGDMLFKPIDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGE
                     FSDGDAGGDPLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAE
                     GTKPRKVLQQ"
     gene            complement(17998..18426)
                     /locus_tag="ACL297_001275"
     CDS             complement(17998..18426)
                     /locus_tag="ACL297_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HGR0071535.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            18564..19706
                     /locus_tag="ACL297_001276"
     CDS             18564..19706
                     /locus_tag="ACL297_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGR0071536.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19715..20929
                     /gene="thiI"
                     /locus_tag="ACL297_001277"
     CDS             19715..20929
                     /gene="thiI"
                     /locus_tag="ACL297_001277"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGR0071537.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21064..21888
                     /locus_tag="ACL297_001278"
     CDS             21064..21888
                     /locus_tag="ACL297_001278"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HGR0071538.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            21901..23067
                     /locus_tag="ACL297_001279"
     CDS             21901..23067
                     /locus_tag="ACL297_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGR0071539.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            23575..25068
                     /locus_tag="ACL297_001280"
     CDS             23575..25068
                     /locus_tag="ACL297_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGR0071540.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25081..>25588
                     /locus_tag="ACL297_001281"
     CDS             25081..>25588
                     /locus_tag="ACL297_001281"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGR0071541.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLL"
BASE COUNT         7641 a         4714 c         5606 g         7627 t
ORIGIN      
        1 aaaatccttt gtttactaaa caagggattt ttcttttgtc tctgctcctt ttttgatata
       61 atagttctat gttaaaatca gaaaaacaat cacgttatca aatgttaaat gaagaattgt
      121 ccttcctatt ggaaggcgaa accaatgttt tggctaatct ttccaacgcc agtgctctca
      181 taaaatcacg ttttcctaat accgtatttg caggctttta tttgttcgat ggaaaggaat
      241 tggttttagg ccccttccaa ggaggtgttt cctgcatccg tattgcacta ggcaagggtg
      301 tttgtggtga ggcagctcac tttcaggaaa ctgttattgt tggagatgtg acgacctatc
      361 tcaactatat ttcttgtgat agtctagcta aaagtgaaat tgtggtgccg atgatgaaga
      421 atggtcagtt acttggagtt ctggatctgg attcttcaga gattgaggat tacgatgcta
      481 tggatcgaga ttatttggaa caatttgtcg ctattttgct tgaaaagaca acatgggact
      541 ttacgatgtt tgaggaaaaa tcttaatgta tcaagcactt tatcgaaaat atagaagtca
      601 aaacttctcc cagttagttg gtcaagaagt tgtggctaag actcttaaac aagcggtgga
      661 gcaagagaaa ataagtcacg cttatctttt ttctggtcct cgtggaacgg gaaaaaccag
      721 tgttgctaaa atctttgcca aggctatgaa ctgtcccaat caagtgggtg gcgaaccttg
      781 caataactgc tatatttgtc aagcagtgac ggacggtagt ttagaagatg tcattgaaat
      841 ggatgcagct tctaataatg gggtagatga aattcgcgaa attcgtgata aatctaccta
      901 tgcgcctagc cttgctcgtt ataaggttta tatcatagat gaggttcaca tgctgtctac
      961 aggggctttt aatgccctcc taaagacgct ggaagaacca acacagaatg tagtctttat
     1021 tttggccact actgaattgc acaagattcc tgctactatt ctatcccgtg tgcaacgttt
     1081 tgagtttaaa tcaattaaga cacaggatat taaggaacat attcactata tcttagaaaa
     1141 agaaaatatc agttctgaac cagaggctgt ggaaatcatt gccaggcggg cggaaggtgg
     1201 aatgcgggac gccttgtcta ttttggatca agccctgagt ttgacacagg gaaatgagct
     1261 gacgactgct atctctgaag aaattactgg caccattagc ctatcagcct tggatgatta
     1321 tgtggcggcc ttgtctcaac aggatgttcc caaagctttg tcttgcttga atcttctttt
     1381 tgacaatggt aagagcatga ctcgttttgt gaccgatctt ttgcactatt taagagactt
     1441 gttaattgtt caaacagggg gagaaaatac tcatcatagt tcagtctttg tagaaaattt
     1501 ggcacttcct caaaaaaatc tgtttgaaat gattcgctta gcaacagtga gtttagcaga
     1561 tattaagtct agtttgcagc ccaagattta tgctgaaatg atgaccgtcc gtttggcgga
     1621 aatcaagccc gaaccagctc tatcaggagc ggttgaaaat gaaattgcta cgctgagaca
     1681 ggaagttgcc cgtctcaaac aagagctttc taatgtaggt gcggttccta aacaagttgc
     1741 accagctcct agtcgaccag ctacgggcaa aacagtctat cgtgtcgatc gcaataaagt
     1801 gcaatctatc ttacaagagg ccgtcgaaaa tcctgattta gcacgtcaaa atctaattcg
     1861 tttgcagaat gcctggggag aggtaattga aagtctaggt gggccggaca aggctctgct
     1921 agttggttct caaccggttg ctgccaatga acaccatgct attcttgctt ttgagtctaa
     1981 cttcaatgct ggtcaaacta tgaaacgaga caatctcaat accatgtttg gtaatatcct
     2041 cagtcaggcg gcaggttttt cacctgagat tttagctatt tccatggagg aatggaaaga
     2101 agttcgcgca gccttttcag ccaaagccaa atcttctcaa actgaaaaag aagtagaaga
     2161 aagcctgatt ccagaaggat ttgaattttt ggctgataaa gtgaaggtag aggaagacta
     2221 aagaaagatt tcatgataca ataagtttat gaataaacaa caatttatta ttatggcgct
     2281 gtttacagct gctgagacct attttttcaa tgaagcctgg atgactggcc gctatattat
     2341 ggcagccttt tgggcaattt tactctttag aaatttccga gtcagttatg tgatgggcaa
     2401 aatcgttgat gtcatcgatc agcattttaa taggaaagac tagccctcag cttccagaca
     2461 aaatcaaagc cttttaggct tttttttgtt atactagaaa agtatattta tagaattttt
     2521 gctctatttc tggggaaatc agacgttttt ctagtaagta ctgtaaaagt tttgaaaaag
     2581 aaaggaacta tcatgtcagt attagagatt aaagatcttc acgttgagat tgaaggaaaa
     2641 gaaattttaa aaggggttaa cctgaccctg aaaacaggag aaattgccgc tatcatggga
     2701 ccaaatggta caggtaaatc gactctttct gccgctatca tgggaaatcc aaactatgaa
     2761 gtaactaaag gtgaagtttt gtttgatggc gtaaacatcc ttgagttgga agtggatgag
     2821 cgtgcgcgta tgggactttt ccttgctatg caatacccat cagaaatccc tggaattacc
     2881 aatgctgagt ttcttcgtgc cgctatgaat gcgggtaaag aaggtgatga gaagatttca
     2941 gttcgtgagt ttattactaa gctagatgaa aaaatggaat tgctcaacat gaaagaagaa
     3001 atggcagagc gttacctcaa cgaaggcttc tctggtggtg agaaaaaacg caatgaaatt
     3061 cttcaacttt tgatgttgga gccaacattt gctcttttgg acgagattga ctcaggtctt
     3121 gatattgacg ctcttaaagt tgtgtctaaa ggtgtcaatg ccatgcgtgg tgaaggtttt
     3181 ggtgctatga tcatcactca ctaccaacgt cttttgaact atatcacacc tgatgtggta
     3241 cacgtgatga tggaaggtcg tgttgtcctt tctggtggtc cagaattggc tgcgcgtttg
     3301 gaacgtgaag gatacgcaaa attagctgaa gaacttggct acgactacaa ggaagaattg
     3361 taatttcctc gtatctttta ggagaagtaa atgactagag aaaatattaa acttttttca
     3421 gaaatgcacg ctgaaccaag ctggttggct gatctccgtc aaaaagcttt tgacaagatt
     3481 gagactttgg aattaccagt tattgagcgt gtcaaattcc accgttggaa tctgggtgat
     3541 ggaacgatta cagaaaatga gccatcagca aatgttccag atttcacagc tttagatcat
     3601 cacttgaagt tggtgcaagt aggaactcaa actgttttcg aacaaactcc agttgagtta
     3661 gctgaacagg gtgttgtctt cacagacttt cactcagctt tagaagaaat tccagagctg
     3721 atcgaagaat tcttcatgtc atctgttaag tatgatgatg acaagttggc ggcttaccac
     3781 acagcttact ttaacagtgg tgctgtactc tatattccag ataacgtaga aatcacagag
     3841 ccaattgaag gaattttcta ccaagatagc gatagcaatg tgccgtttaa caagcatatt
     3901 atgattatcg ctggtaaaaa ttctaagatt agttatctgg agcgtttaga gtcacgcggt
     3961 gaaggaagtg acaaagcaac tgccaatatc acagtggaag tgattgcacg ttctggtgcg
     4021 caagtcaagt ttgctgctat tgaccgtcta ggtgaaaacg tcactgccta cattagccgt
     4081 cgtggtaaat taggcaacga tgcaagtatt gactgggcta tcggtgtcat gaacgaagga
     4141 aatgtcgttg ctgattttga tagtgacttg attggtaatg gtagccatgc tgacctcaag
     4201 gttgtagctc tttcaagtgg tcgtcaggta caagggattg atactcgtgt aactaactat
     4261 ggctgcaact ctatcggaaa tatcctacaa catggggtta tccttgaaaa agcaactttg
     4321 actttcaatg gtatcggcca catcatcaag ggtgctaagg gagcagatgc ccaacaagag
     4381 agccgtgttc tcatgctttc agaccaagcg cgttcagatg ctaacccaat tcttttgatt
     4441 gatgaaaatg acgtcactgc aggacatgca gcctctattg gtcaggtaga tccagaagat
     4501 atgtactacc tcatgagtcg tggattggat aaggcaactg cagagcgttt ggttgttcgt
     4561 ggtttccttg gatctgttat cgtggagatt ccagtcaagg aagttcgtga tgaaatgatt
     4621 gcaactatcg aagagaaatt gtcaaaacgc taaggggaag cctatgttag atgtagaagc
     4681 gattcgcaag gattttccaa ttttagatca gattgtcaat gatgaacctc tggtctatct
     4741 ggacaatgct gcgacgacac aaaaaccact agtagttctg aaagctatta acagttacta
     4801 tgagcaggac aatgccaatg ttcaccgtgg tgtccatacc ttagcggaac gagcgacagc
     4861 ttcttatgaa gctgctcgtg aaaccattcg taagtttatt aatgcaggct ctacaaagga
     4921 agttctcttt accagaggaa cgacaaccag ccttaactgg gtggcacgct ttgctgagga
     4981 aattctcact gagggagacc aggtcttgat ttcagtaatg gaacaccatt ctaatatcat
     5041 tccatggcag gaagcttgtc gaaagactgg agcagagctt gtctatgtct atcttaaaga
     5101 cggtgccttg gatatggagg atttgcgagc taaattgact gataaggtta aatttgtttc
     5161 cctagctcat gcctccaatg ttcttggtgt ggtcaatccg atcaaggaaa tcactcaatt
     5221 agcccaccaa gttggggcaa tcatggtagt ggatggtgct caatctacac ctcatatgaa
     5281 gattgatgtc caggacttgg atctggactt tttcgccttt tcgggtcaca agatggctgg
     5341 tccgactggt atcggtgtcc tttacggcaa agaaaagtat cttgagcaaa tgtctccagt
     5401 agaatttggc ggcgagatga ttgattttgt ctacgagcaa tctgctagtt ggaaggaatt
     5461 gccttggaaa tttgaggctg gaacgccaaa tatggcagga gctattggac ttgcgactgc
     5521 agttgattat ctggaaaaga ttggtatgga tgccgttgaa gctcatgaac aggaattgat
     5581 tgcgtacgtc tatccaaaac tgcaggcaat tgagggattg accatttacg gttctcagga
     5641 tttggctcaa cgttcgggtg ttattgcctt taacctaggt gatctccatc ctcacgatct
     5701 tgcgacagct ctggattatg aaggagtggc tgttcgtgct ggtcaccatt gtgcgcaacc
     5761 cttgcttcag tatttggaag tcccagcaac agctcgtgca agtttttata tctataatac
     5821 caaggcagat tgcgacaaac tagtcgatgc cctacaaaag acaaaggagt ttttcaatgg
     5881 cactttctaa actagatagc ctttatatgg cagtggtagc agaccattcg aaaaatccac
     5941 atcaccaagg gaagttagaa gacgctgagc aaatcagtct caacaatccg acttgtggag
     6001 atgtcatcaa tctctctgtc aagtttgatg cagaggaccg tttggaagat attgcttttc
     6061 taaattcagg atgcacgatt tcaactgctt ctgctagtat gatgacagat gccgttttag
     6121 gaaaaaccaa acaagaaatt ttagaactgg cgactatttt ttctgaaatg gttcaagggc
     6181 aaaaagatga gcgtcaagac caacttggag acgcggcatt cttgtcaggt gttgccaaat
     6241 tccctcaaag aatcaagtgt gcaaccctag cttggaatgc ccttaagaaa acaattgaaa
     6301 atcaagaaaa acagtaagac aagtttcttt tgtcttatga attattagaa atgaagaaag
     6361 aaaggatatt atggctgaag aaagagtaga accaaaacca attgaccttg gtgaatataa
     6421 atttggtttc catgacgatg tagagcctgt cttatcgaca ggaaaaggac tcaacgaagg
     6481 tgttattcgt gaattatctg ctgctaaggg tgagcctgag tggatgttgg agttccgttt
     6541 gaagtcttat gaaaccttca aaaaaatgcc catgcaaact tggggagcag acttgtcaga
     6601 gattgacttt gatgacttaa tctactacca aaaaccatct gacaaaccag cccgttcttg
     6661 ggatgatgta cctgaaaaga ttaaagaaac ctttgaacgt atcgggattc cagaagctga
     6721 acgtgcttat ttagcagggg cttctgccca gtacgagtca gaagtggttt accacaacat
     6781 gaaggaagag ttccaaaaat taggtattat ctttacagat acagattccg cactcaagga
     6841 atacccagac ttatttaatc aatactttgc gaagttggta ccgccgacag ataacaagtt
     6901 ggcagccctc aactcagcag tatggtcggg tggaactttt atctacgtgc caaaaggtgt
     6961 caaggtagat attccacttc aaacttattt ccgtatcaat aacgaaaata taggtcagtt
     7021 cgaacgtacc ttgattatcg ttgatgaggg agcaagcgtc cactacgtag aaggatgtac
     7081 agcaccaaca tattcaagca atagcttaca cgctgccatt gtagaaattt ttgctttgga
     7141 cggagcttat atgcgttata caactatcca aaactggtct gataacgtct ataacttggt
     7201 aacaaagcgt gctaaggctc aaaaggatgc cactgttgag tggattgatg gaaacttggg
     7261 tgccaaaacg actatgaaat atccatctgt ttaccttgat ggagaaggag cgcgtggtac
     7321 catgctctct atcgcctttg ctaatgcagg gcaacaccaa gacacgggtg ctaagatgat
     7381 tcacaatgct ccacatacca gctcgtctat tgtgtctaaa tccatcgcta aaggtggagg
     7441 aaaggttgac taccgtggac aagtcacctt taacaagaac tctaagaaat ctgtttccca
     7501 cattgaatgt gataccatta tcatggatga cttgtcagca tcagatacta ttccatttaa
     7561 tgaaattcac aactcgcaag tggctttgga acacgaagcc aaagtatcta agatttcaga
     7621 agagcaattg tattatctca tgagccgtgg attgtcagaa tctgaggcaa ctgaaatgat
     7681 tgtcatggga tttgtagaac cctttacaaa agaacttcca atggaatacg cagttgagct
     7741 gaaccgcttg attagctatg aaatggaggg atcagttgga taaaatttga ttttatactc
     7801 ttcgaaaatc tcttcaaacc acgtcagcat cgccttaccg tatgtatggt tactgacttc
     7861 gtcagtttca tctacaacct caaaacagtg ttttgagcaa cctgcggcta gcttcctagt
     7921 ttgttctttg attttgaaga tgaacttgta tcaaaaaatc gcggtttaaa atcgcgattt
     7981 tttataattt ctcgttaaca aagcggacaa actgattcca ccaaactttt aagaagaagg
     8041 ctttttcaat tttcttgtct gctaccattt cgaaactagg gcgctctgtg gtgatgtaac
     8101 cttgaccaat caagtccttg tcttcataag tcaaatggcc aaccactgtt ccagcttcaa
     8161 gtggtgctgg gattgctttg gaatcaggtg tgaattgaac agattgggaa gattgattcc
     8221 caacacgttc tattagatag atatcctctg gagccactgc agttactgta tcttcttttc
     8281 catcttgtac aggggttttg ctatcttgat aggcatcgcc ttgttgaacg attttgcgaa
     8341 gtgtaaatgt agaagaaata taatccatta gggaagatgt agctgtaaat cgagcgtaag
     8401 gattattgtc ttgatgatct gcatttaaaa caactgtgat gactctcatg cctttttcga
     8461 cagtagtacc aacaaaagac tctccagcct tatctgttgt tcctgttttt agcccatcaa
     8521 aaccaccacg gtaagcaggc ataccttcta acatgtagtt ggttgaagtg attgtcatcc
     8581 cagcaaaagt agaagaaggt tttttggtga tttctaagac ttgtgggtat tttttgatga
     8641 ggttgcgagc aacgatagcg acatcataag cactaagctt attttcctca tcttttttag
     8701 aacctgggta aatgttatcc cctagagttt cattgttaag acctgtcgta ttgacaacag
     8761 tggcatcctg aattccccat tccaagagtt ttgcccgcat catatcgacg aaatcttttt
     8821 ctgagccagc aattttctca gctagggcaa tagcggcgct gttggcacta gataccagag
     8881 ttgcttcaag caactcttcg acagtataat tacgggcctc cataggaata ttactggctt
     8941 cagaatttgt cgtcaattga taaggataat cagaaatatc tacaggagtg gagagggtaa
     9001 tacttccgtt ttccaaagct tcatagacca gataaacagt aatcaatttt gttatggaag
     9061 caatttcgac aggttgcgtt gcatccttct catagagaat tttaccagta tttgcctcaa
     9121 cagcaatcgc atgtttagcg gcaatggtaa aatcttgagc aacagcagta gaagcacccc
     9181 ctaaaagaga gacagttaac aaagttaaaa atattttttt catagtagtc ttattctatc
     9241 ataaagaaaa aaaatattct tgctttaata attcatctgt taagcttttt gaaaatatgg
     9301 taaaataaag tgagggaggt aactcatgtt tcgtagaaat aaattatttt tttggaccac
     9361 agaaatttta ctcttaacca tcatctttta cctatggaga cagatgggat ctttgattaa
     9421 cccttttgtt agcgtgctta atacaattat gattccattt ttattagggg gctttcttta
     9481 ttatttgaca aaccctattg ttactttctt aaataaagtc tgtaaactca atcgtttgct
     9541 tggtatttta attaccttgt gtactttggt ctggggaatg gtcataggtg ttgtctatct
     9601 cttacctatt ttgattaatc agttatctag tttgattata tctagtcaaa ctatttatag
     9661 tcgagtacaa gacttaatca tagacttatc taattatcct gcgctccaga atttggatgt
     9721 agaagctaca attcagcagt taaacttatc ctatgttgat attcttcaaa atatcctaaa
     9781 tagcgtatca aatagtgtgg ggagcgtctt gtcagctctt atcagtactg ttttgatttt
     9841 gattatgact ccagtttttt tggtttattt cttattagat ggacataaat tcttgcccat
     9901 gcttgaaaga acgattctaa agagggatcg cttgcatatt gcaggcttat taaagaattt
     9961 aaatgcgacg attgctcgct atattagtgg agtttcgatt gacgcaatca ttataggttg
    10021 tttggcttat attggctata gtattattgg tttaaaatat gctttagttt ttgccatttt
    10081 ttctggtgta gccaatttaa ttccttatgt ggggccaagt attggtttga ttcctatgat
    10141 catcgcaaat atattcactg atccccatag actgctgact gcagtgattt atatgcttgt
    10201 tgttcagcag gtagatggca atatcttata tcctcgaatt gtaggaagtg ttatgaaggt
    10261 tcatccaatc acgattttag ttttactttt gttgtcaagc aatatctatg gtgtagttgg
    10321 aatgattgtc gcagtgccaa cctattctat cttgaaagaa atttctaagt tcttatcccg
    10381 tttgtatgaa aatcataaaa taatgaaaga acgagaaaga gaattagcta agtaaaagtc
    10441 aggagaaccc tgatttttct ttactggaag tggcctttag attagaagac tgaaaataag
    10501 ttaaagtctt aaactaattt tcacagctaa gaatagtaga agttaatctg ataaaaatcg
    10561 aaaaaaccag tggaattccg tgtcagggta agttccactg gttttcatag tctattaaag
    10621 ttcgaatgaa acctatttat aatatattga aactagaata gtacacctct aattctaaaa
    10681 cattgttaga aatcgatttg actgtcctga tctattcgta ctattcttat ttcattttac
    10741 tatattttgg tgcaataagt gaaaagtagt ccgaataata taaggattga ttttatagtt
    10801 tttaaactca aatgaattaa aataaagaga gtacgaaaat tctcatctga aagtatttta
    10861 gaataattct cttcgtgaat ttcttcaaaa cagatagctt catcttaggt atgtgttaat
    10921 tggatttcgt aagctctatt tacaatcgta aagttatgca ctgagaaagt tgtagttagt
    10981 cttccagttt gctaaatttc tcgaatagta tcttttttag gtatgtgatt tctttttgca
    11041 tttttgagtt agataaggta taatggtttt attgtctttt ggggtcgtta cggattcgac
    11101 aggcattatg aggcatattt tgcgactcgt gtggcgacgt aaacgctcag ttaaatataa
    11161 ctgcaaaaaa taacacttct tacgctctag ctgcctaaaa accagcaggc gtgacccgat
    11221 ttggattgct cgtgttcaat gacaggtctt attattagcg agatacgatt aagccttgtc
    11281 tagcggcttg ataagagatt gatagactcg cagtttctag acttgagtta tgtgtcgagg
    11341 ggttgttaaa ataatacata acctatggtt gtagacaaat atgttggcag gtgtttggac
    11401 gtgggttcga ctcccaccgg ctccattatt cctttgcatt cttttgcatt ccttggtaaa
    11461 acgttgttaa atcaacgttt tttattttta tctttggtat tcctttgcat tcttttgcta
    11521 aaaagggagt cacaaacaga ccctatttta aaaaaaggat agaaaaaagg atacaacatt
    11581 tgtcgcatcc tagaaataat cttttttcga cggaagacat gggattcgaa cccacgcacg
    11641 ctattacacg cctaccgcgt ttccaacacg gcctcttaag cctcttgagt aatcttccaa
    11701 tacttactca aatagtctac cataaaggct cttatcttgc aataaaaatt ctagaaataa
    11761 gaaaaaatga tagattttga aagaaaatga taaaaaatgc ttgacttcga aagaaagtat
    11821 gatagaatga atagtgtaaa cgataacagg aggtgattca gtgttaaaaa cagaacgtaa
    11881 acaactaatt ttagaggagt taaatcaaca tcatgtagtt tctctagaaa aattagttag
    11941 tttgctagaa acgtcagaat caacggttcg aagagacttg gatgagttgg aagcggaaaa
    12001 caagcttcgt cgtgtgcatg gtggagcaga actcccccac tccttacagg aagaagaaac
    12061 cattcaagaa aaatctgtca aaaaccttca agaaaagaag ttgctggctc agaaagcagc
    12121 ctctctcatt aaagaaaaag atgtcatctt tatcgatgct ggaacaacaa ctgctttttt
    12181 gattcatgaa ttggtcaata agaatgttac agttgtgacc aactccattc accatgccgc
    12241 tcagttggtt gaaaagcaga ttccaactgt catggttgga ggaaacgtca agacggcgac
    12301 agatgctagt atcgggggcg ttgctcttaa ccagattaac caattgcact ttgaccgtgc
    12361 ctttatcgga atgaatggtg ttgacgatgg ctattatacg actcctgata tggaggaagg
    12421 agctgtgaaa agagctattt tggagaatgc caagcagacc tacgtcttgg tggattcgtc
    12481 aaaaattgga caaacttgct ttgccaaggt agcaccactc aaacgcgcta tcgttatcac
    12541 tagtcaaggg catgagctct tgcaggttat taaggagaaa acggaggtaa tagaagtatg
    12601 atttatacag tcacactcaa tccatccatt gactatatcg ttcgtttgga ccaagtcaaa
    12661 gttggtagtg taaatcgtat ggacagtgat gataagtttg ctggtgggaa aggaatcaat
    12721 gtcagccgtg tcttgaaacg tttgaatata ccaaatacag cgacgggatt tatcggtggc
    12781 tttactggta aatttatcac agatacttta gcagaggaag aaatcgagac acgttttgtc
    12841 caggtggcag aagatactcg tatcaatgtt aaaatcaaag cagaccaaga aacagaaatc
    12901 aacggaacgg gtccaactgt tgaaccggtt cagctagaag aattgaaagc tattttatct
    12961 agtctgacag cagaagatac agttgtcttt gcaggttcaa gtgctaaaaa tctaggcaat
    13021 gttatctata aggatttgat ttccttgacg cgccagactg gtgcgcaagt ggtctgtgac
    13081 tttgaaggac agaccttaat tgatagtttg gactaccagc ctcttcttgt aaaaccaaac
    13141 aatcatgaac ttggagcgat ttttggggtt aaactcgaaa gtttagatga aattgagaaa
    13201 tacgctcgtg agttactggc taagggtgct caaaatgtta ttatctctat ggctggtgat
    13261 ggcgcccttc ttgtcacatc tgagggagct tacttcgcta aaccaatcaa aggaacagtc
    13321 aaaaattcag ttggagctgg tgattctatg gttgctggat tcacaggtga atttgtcaaa
    13381 tcaaaagacg cagtagaagc cttcaaatgg ggagtggctt gcggaacggc aactaccttc
    13441 tcagatgact tggcaacggc ggaatttatt aaagaaacat atggaaaagt tgaggtagaa
    13501 aaaagatgaa aattcaagac ctattgagaa aagatgtcat gttgctagat ttgcaggcaa
    13561 ctgaaaaaac agctgtcatc gacgagatga ttaaaaattt gacagagcac agttatgtaa
    13621 cagattttga aacatttaaa gaaggaattt tggcgcgtga agctttgact tctactggtt
    13681 tgggtgatgg aatcgcaatg cctcacagca aaaacgctgc tgtcaaagaa gcgacagttc
    13741 tatttgctaa gtcaaataag ggtgttgact acgagagctt ggatggacaa gcaactgacc
    13801 tcttcttcat gattgcagct ccagaaggtg ccaatgatac tcacttggca gccttggcag
    13861 aattgtctca atacttgatg aaagacggtt ttgcagacaa acttcgtcaa gcaacatctg
    13921 cagaccaagt tatcgaactt tttgaccaag cttcagaaaa aactgaggaa cttgttcaag
    13981 tacctgctaa tgactctggt gactttatcg tagctgttac agcttgtaca acaggtattg
    14041 cccacactta catggcccaa gaagcccttc aaaaagtagc tgctgaaatg ggggttggta
    14101 tcaaggtcga aaccaacggt gctagcggtg ttggaaatca actaactgca gaagatatcc
    14161 gtaaggctaa agctattatc attgcagcag acaaggccgt tgaaatggat cgatttgatg
    14221 gaaaaccatt gatcaatcgt ccagttgctg acggtatccg taagacagaa gagctaatta
    14281 acttggctct ttcaggagat actgaagtct accgtgccgc taatggtgca aaagctgcaa
    14341 cagcctctaa cgaaaaacaa agccttggtg gtgccttcta caaacacttg atgagtggtg
    14401 tatctcaaat gttaccattc gttatcggtg gtggtatcat gattgccctt gccttcttga
    14461 ttgacggtgc tttgggtgtt ccaaatgaaa accttggcaa tcttggttct taccatgagt
    14521 tagcttctat gttcatgaaa attggtggag ctgcctttgg tttgatgctt ccagtctttg
    14581 cgggttatgt tgcctactct attgctgaaa aaccaggttt ggtagcaggt ttcgtggctg
    14641 gtgctattgc caaagaaggt tttgcctttg gtaaaattcc ttatgccgca ggtggtgaag
    14701 caacttcaac tcttgcaggt gtctcatctg gtttcctagg tgcccttgtt ggtggattta
    14761 tcgcaggtgc cttggttctt gccatcaaga aatacgttaa agttcctcgt tcactcgaag
    14821 gtgctaaatc aatccttcta ttgccacttc ttggaacaat cttgacagga tttgttatgc
    14881 tagctgtgaa tatcccaatg gcagcaatca acactgctat gaatgacttc ctaggcggtc
    14941 ttggaggagg ttcagctgtc cttcttggta tcgtccttgg tggaatgatg gctgttgaca
    15001 tgggtggacc agttaataaa gcagcttatg tctttggtac aggtacgctt gcagcaactg
    15061 tttcttcagg tggttctgta gccatggcag cagttatggc tggaggaatg gtgccaccac
    15121 ttgcaatctt tgtcgcaact cttcttttca aagataaatt tactaaggaa gaacgtaact
    15181 ctggtttgac aaacatcatc atgggcttgt catttatcac tgagggagcg attccatttg
    15241 gtgccgctga cccagctcgt gcgattccaa gcttcatcct tggttcagca gtagcaggtg
    15301 gactcgttgg tcttactggt attaaactca tggcgccaca cggaggaatc ttcgttatcg
    15361 cccttacttc aaatgctctc ctttacctcg tttctgtctt ggtaggagca atcgtaagtg
    15421 gtgtggttta tggttaccta cgcaaaccac aagcataaaa aatagaaaaa tgaaaagatt
    15481 ggaccgtttg gtgcagtctt tttctctttc cgaaatgcct gtgaaatatg gtataataga
    15541 agaatggcaa acaagaatac aagtacaaca agacggagac cgtctaaagc agaactggaa
    15601 agaaaagaag cgattcaacg aatgttgatt tcgttaggaa ttgcgatttt attgattttc
    15661 gcagccttca aattaggggc tgcaggtata accctttata atttaattcg cttgctagtg
    15721 ggtagcctag cttatctggc gatattcggc ctattaatct atctcttttt tttcaagtgg
    15781 atacgaaaac aggaaggact cttatctggc tttttcacca tatttgctgg cttactcttg
    15841 atttttgaag cctacttggt ttggaaatat ggtttggaca agtccgttct aaaagggacc
    15901 atggctcagg ttgtgacaga tctgactggt tttcgaacga ctagttttgc tggagggggc
    15961 ttgatcgggg tcgctcttta tattccaaca gcttttctct tttcaaatat cggaacttac
    16021 tttattggtt ctatcttgat tttagtgggt tctctcctag tcagcccttg gtctgtttac
    16081 gatattgctg aatttttcag tagaggcttt gccaaatggt gggaagggca cgagcgtcga
    16141 aaagaggaac gctttgtcaa acaagaagaa aaagctcgcc aaaaggctga gaaagaggct
    16201 agattagaac aagaagagac tgaaaaagcc ttactcgatt tgcctcctgt tgatatggaa
    16261 acgggtgaaa ttctgacaga ggaagctgtt caaaatcttc cacctattcc agaagaaaag
    16321 tgggtggaac cagaaatcat cctgcctcaa gctgaactta aattccctga acaggaagat
    16381 gactcagatg acgaagatgt tcaggtcgat ttttcagcca aagaagccct tgaatacaaa
    16441 cttccaagct tacaactctt tgcaccagat aaaccaaaag atcagtctaa agagaagaaa
    16501 attgtcagag aaaatatcaa aatcttagaa gcaacctttg ctagctttgg tattaaggta
    16561 acagttgaac gggccgaaat tgggccatca gtgaccaagt atgaagtcaa gccggctgtt
    16621 ggtgtaaggg tcaaccgcat ttccaatcta tcagatgacc tcgctctagc cttggctgcc
    16681 aaagatgtcc ggattgaagc accaatccct gggaaatccc taatcggaat tgaagtgccc
    16741 aactccgata ttgccactgt atctttccga gaactatggg aacaatcgca aacgaaagca
    16801 gaaaatttct tggaaattcc tttagggaag gctgttaatg gaaccgcaag agcttttgac
    16861 ctttctaaaa tgccccactt gctagttgca ggttcaacgg gttcagggaa gtcagtagca
    16921 gttaacggca ttattgctag cattctcatg aaggcgagac cagatcaagt taaatttatg
    16981 atggtcgatc ccaagatggt tgagttatct gtttacaatg atattcccca cctcttgatt
    17041 ccagtcgtga ccaatccacg caaagccagc aaggctctgc aaaaggttgt ggatgaaatg
    17101 gaaaaccgtt atgaactctt tgccaaggtg ggagttcgga atattgcagg ttttaatgcc
    17161 aaggtagaag agttcaattc ccagtctgag tacaagcaaa ttccgctacc attcattgtc
    17221 gtgattgtgg atgagttggc tgacctcatg atggtggcca gcaaggaagt ggaagatgct
    17281 atcatccgtc ttgggcagaa ggcgcgtgct gcaggtatcc acatgattct tgcaactcag
    17341 cgtccatctg ttgatgtcat ctctggtttg attaaggcca atgttccatc tcgtgtagca
    17401 tttgcggttt catcaggaac agactcccgt acgattttgg atgaaaatgg agcagaaaaa
    17461 cttcttggtc gaggagacat gctctttaaa ccgattgatg aaaatcatcc agttcgtctc
    17521 caaggctcct ttatctcgga tgacgatgtt gagcgcattg tgaacttcat caagactcag
    17581 gcagatgcag actacgatga gagttttgat ccaggtgagg tttctgaaaa tgaaggagaa
    17641 ttttcggatg gagatgctgg tggtgatccg ctttttgaag aagctaagtc tttggttatc
    17701 gaaacacaga aagccagtgc atccatgatt cagcgtcgtt tgtcagttgg atttaaccgt
    17761 gcgacccgtc tcatggaaga gcttgagatg gcaggtgtca ttggtccagc tgaaggtacc
    17821 aagccaagaa aagtattgca gcaataaaaa atagcttctt tccaaggttg gaaggagcta
    17881 ttttactggc tattgattac ttttatactc ttcgcaaatc tcttcaaacc acgtcagctt
    17941 cgccttgccg tactcaagta ctgtctacgg ctagcttcct agtttgctct ttgattttca
    18001 ttgagtatta ttttctgaag ttggagtatt gggctgtttt tcattttcag tggcaggttt
    18061 acttgtagca ggtgtagtag agtcctgagt tgctgttttc tgatcttctt ttttctcttc
    18121 cttgacgcta gattttggcg tttcttcttg ttgtgttttt tcttgagaag tgttattttc
    18181 cttatttgga gtagtatttt cctgagggga ttctttttga atttctttga caaaggttgt
    18241 cgtctggctt gtcgtaggtt cttttttagt atttttatta ttattatcca aggcgttcat
    18301 gatcgtgata cccgcggtaa tcaaaccaag gatactaccg atagtagtaa tggttttttg
    18361 aaaaacagta aagccttttt tgatatgttc tgttttcggt tttgaagttc tacgatagtt
    18421 agacatacat tctctccttt gattatgatt tattatactc tgtttcttta aaaaaatctt
    18481 aaaaaaagag aaattgccct ttcttgtcgc aggctccgtt tcatgataca atagaaggag
    18541 tgattttaga aagcgtgaga aacatgattt actttgataa ttcggcgacg accaggccct
    18601 atccagaagc ccttgaaacc tatatgcagg tggcttcaaa aattttagga aatccatcta
    18661 gtctccatcg tttgggagac caggcaacac gaattttaga tgcttcccgt caacagattg
    18721 cagatttaat cggtaagaaa agtgatgaaa tcttctttac atctggtggg acagaagggg
    18781 ataactggat catcaaggga gtagcatttg aaaaagccca gtttggcaag cacatcatcg
    18841 tatcagccat tgaacatcca gcagttaagg aatcagccct ctggttgaaa gctcaaggtt
    18901 ttgaagtgga ttttgctcca gttgataaca aaggatttgt ggatgttgag gcgctagaag
    18961 atttgataag acctgatacg accctcgttt ccatcatggc tgtgaacaat gaaatcggct
    19021 ctatccaacc tattgaggct atttcagaat tcttggcaga caagccaact atttccttcc
    19081 acgttgatgc ggttcaggcg cttgccaaga ttccgactga aaagtatctg acagaacgag
    19141 tggatttcgc gactttctct ggtcacaagt tccacggaat ccgtggtgtt ggttttatct
    19201 atatcaagtc tggcaagaag attacgcctc ttttaacagg tggtggtcag gaacgtgatt
    19261 accgttcgac aactgaaaat gtggcaggaa ttgcagcgac cgctaaggct ctccgtttat
    19321 ctatggaaaa gctagatatc tttaggagca agactgggca gatgaaggca gtgattcgtc
    19381 aagcacttct ggactatccg gatatatttg tcttttcaga tgaggaagac ttcgcccctc
    19441 atattctgac ttttggaatc aagggtgttc gtggtgaggt cattgttcac gcctttgaag
    19501 actatgatat tttcatctca acgacctcgg cttgttcgtc caaggctggg aaaccagccg
    19561 gaaccctgat tgctatggga gtggacaagg ataaggctca gtcagctgtg cgtcttagtc
    19621 tagaccttga aaatgatatg agtcaggtcg agcagttctt gaccaagcta aaattaattt
    19681 acaatcaaac tagaaaagta agataggagc attcatgcag tattcagaaa ttatgattcg
    19741 ctacggtgag ctgtcaacca agggcaaaaa tcgtatgcgt ttcatcaata aacttcgcaa
    19801 taatatttca gacgttttgt ctatctatcc ccaagttaag gtaacagcag atcgcgaccg
    19861 tgcccacgct tacctcaatg gagcagatta cacagcagta gcagaatcgc tcaaacaagt
    19921 atttggaatt caaaactttt cccctgttta taaggttgaa aaatctgtag aagttctgaa
    19981 gtctgctgtc caagagatta tgcaggacat ctacaaggaa ggcatgacct ttaaaatctc
    20041 tagcaagcgt agcgaccaca cctttgaatt ggacagtcgt gaactcaacc aaacgcttgg
    20101 aggggctgta ttcgaagcca ttccaaacgt acaagctcaa atgaaaagtc ctgacatcaa
    20161 tcttcaggtg gagatacgtg aagaagcagc ctatctttct tatgaaaccg ttcgaggggc
    20221 aggtggatta ccagtgggaa cttctggtaa gggcatgctc atgttgtcag ggggaatcga
    20281 ttcccctgta gctggctatc tagcgcttaa acgaggggta gatatagagg cagttcactt
    20341 tgccagccca ccttacacga gtcctggtgc tcttaaaaaa gcccaagatt tgacccgtaa
    20401 attaaccaaa tttgggggaa atatccagtt tatcgaagtt ccttttacag agattcaaga
    20461 ggaaatcaaa gccaaagcgc cagaagccta ccttatgacc ttgacgcgtc gttttatgat
    20521 gcgtattact gaccgtattc gtgaggtacg aaatggttta gttatcatca atggggaaag
    20581 tctaggtcaa gtagccagcc aaacccttga aagtatgaag gctatcaatg ctgttaccaa
    20641 cactcccatc attcgtcctg tggttaccat ggacaagttg gaaatcattg acatcgccca
    20701 ggaaatcgat acctttgaca tttcaatcca accgtttgaa gactgttgta ctatttttgc
    20761 accagatcgt ccaaaaacaa atcctaaaat taagaatgcg gagcagtacg aagcgcgtat
    20821 ggatgttgaa ggcttggttg agcgagcagt ggctggaatc atgattactg aaatcacacc
    20881 tcaagccgaa aaagatgaag ttgatgactt gattgacaat ctgctctaat tcagaaaatc
    20941 caaaagaata gcgaaaatca gtaaaaaaag ttagtttttt ctctaaaaac aggtaaaaaa
    21001 ctaacttttt ttatttttat gatataatga tataaaattt tgaatataga gagttttctg
    21061 acaatgaatc aatcctactt ttatctaaaa atgaaagaac acaaactcaa ggttccttat
    21121 acaggtaagg agcgccgtgt acgtattctt cttcctaaag attatgagaa agatacagac
    21181 cgttcctatc ctgttgtata ctttcatgac gggcaaaatg tttttaatag caaagagtct
    21241 ttcattggac attcatggaa gattatccca gctatcaaac gaaatccgga tatcagtcgc
    21301 atgattgtcg ttgctattga caatgatggt atggggcgga tgaatgagta tgcggcttgg
    21361 aagttccaag aatctcctat cccagagcag cagtttggtg gtaagggtgt ggagtatgct
    21421 gagtttgtca tggaggtggt caagcctttt atcaagcaca aaacaggttg gtttgatggc
    21481 atgatgacga caggttgctc tatgggagcc tatcatgcac tcaatttctt cctccagcat
    21541 ccagatgtct ttaccaaagt gattgctctc agtggtgttt acgacgcacg tttctttgtc
    21601 ggcgattact acaacgatga tgctatttac caaaactcgc cagtagatta tatttggaac
    21661 caaaacgatg gctggtttat tgaccgttac cgtcaggcag agattgtgct gtgtacgggg
    21721 cttggagcct gggaacaaga tggtttacca tccttttaca agctcaaaga agcctttgac
    21781 cagaaacaaa ttccagcctg gtttgctgaa tggggacatg atgtcgccca cgactgggaa
    21841 tggtggcgta aacaaatgcc ttatttcctc ggtaatctct atttataaaa ggagttacct
    21901 atgaattacc ttgttatttc tccctactat ccacaaaact ttcaacagtt taccatcgaa
    21961 ctagctaata aaggcatcac agtcttggga attggtcaag agtcttacga gcaattggat
    22021 gagcccttgc gcaatagctt gaccgagtat tttcgtgttg ataatcttga gaacatagat
    22081 gaagtcaaac gtgcagttgc ttttctcttt tataaacatg gtccaattga ccgcatcgag
    22141 tctcacaatg aatactggct tgagctagac gcaacactca gagaacaatt caatgttttt
    22201 ggtgccaaac cagaggatct caaaaagacg aaatataagt ctgaaatgaa gaaacttttc
    22261 aaaaaagcag gtgttcctgt ggtacctgga gctgttatca agacggaagc agatgttgat
    22321 caagcagtga aagaaatcgg tcttccaatg attgccaaac ctgataatgg agtgggagca
    22381 gccgcaacct ttaaacttga gacagaagac gatatcaatc acttcaagca agaatgggac
    22441 cattcaaccc tttatttctt tgaaaaattt gtcacttcca gcgaaatctg tacctttgac
    22501 gggctcgtgg acaaggatgg aaagattgtc ttctcaacaa cctttgacta cgcctataca
    22561 ccgcttgacc tcatgattta taagatggac aattcttatt atgtgctcaa ggatatggat
    22621 cctaaactgc gcaagtatgg ggaagcaatt gtcaaagaat ttggtatgaa agaacggttt
    22681 ttccatattg agttcttccg tgagggggac gattatatta ccatcgagta caataaccgc
    22741 cctgcaggtg gttttaccat tgatgtttat aactttgctc attccttgga cctttatcgt
    22801 ggctatgcag ctattgtcgc aggagaggag ttcccggcgt cagactttga aactcagtat
    22861 tgtttggcta cttctcgccg tgcaaatgct cactatgttt attcagaaga ggatttgctt
    22921 gccaaatata gccagcagtt caaggttaaa aaagtcatgc cagctgcctt cgcggaactt
    22981 caaggagatt acctgtatat gctgacaact ccgagtcgac aagaaatgga tcagatgatt
    23041 gctgatttcg gacaacgtca agaataagaa ctatcggatt aaggaaattc actcccttaa
    23101 tccttttgtt ttgtctggta aaaaataaga gcatcctaac aaggtagcta tcataaaact
    23161 tgttcgataa ctatttgaag caggattagg tggctagaaa ttgaatttta acatttcaat
    23221 ttagtcatag tattgtgttt tcgtatcctt aaatcagcta aaaggatcca tgacgacacc
    23281 tatacgatat agttttcagg ataccaaacc agtctattaa tactcaatga aaatcaaaga
    23341 gcaaactagg aagctagccg caggttgctc aaaacactgt tttgaggttg tggatagaac
    23401 tgacagagtc agtatcatat acctacggca aggtgaagct gacgtggttt gaagagattt
    23461 tcgaagagta taaaatattc aggtgacgca tagatatagt taattgaagc tttgtttgaa
    23521 atctgataaa ataatgatat tactaagttt taaaaactaa agaaaaggga agatatgatt
    23581 acaggcgaat taaaaaataa aatcgatcag ctgtgggaaa ttctttggac agaaggaaac
    23641 gcaaatcctt taacaaatat tgaacagttg acttatctct tatttatgaa agatttggat
    23701 agtgtcgagc ttggacgtga aagtgatgct gaatttctag ggattcctta tgagggagtt
    23761 tttccaaaag ataaacctga ataccgttgg tcaactttta aaaatatagg agatgctcag
    23821 gaagtttatc gtttaatgac tcaggagatt tttccgttta ttaaaaatct caagggggat
    23881 acagatgata cagccttttc acgatatatg cgagaagcta tttttcaaat aaataaacct
    23941 gctacgcttc aaaaggcaat ttctatctta gatgtttttc caactagggg attagatgta
    24001 gattttgata atgacaaaca aagtattact gatatcggag atatctatga atatctgtta
    24061 tcaaaattgt cgaccgcagg taaaaatgga cagttccgta cacctcgtca catcatcgat
    24121 atgatggttg agttgatgca accgactatc aaagatatca tctcagatcc cgctatgggt
    24181 tctgctggct tcttagtatc tgctagccgt tacttaaagc gtaagaaaga tgaatgggaa
    24241 accaatacag ataatatcaa tcattttcat aatcagatgt ttcatggaaa tgatacggat
    24301 acgactatgt tgagacttgg ggcgatgaac atgatgctac atggagtaga aaatccacaa
    24361 atcagttacc ttgactcgct gtctcaagat aatgaagaag ccgataaata tactttggtt
    24421 ttagcaaatc ctccttttaa gggctcactt gactacaatt caacctctaa tgaccttctt
    24481 gcaaccgtaa aaaccaaaaa aacagaatta ctctttcttt ctcttttctt gcgaacttta
    24541 aaaccaggtg gacgagcagc agttatcgta cctgatggtg tcctttttgg ttcgtctaaa
    24601 gctcataaag gaattcgtca ggaaattgta gagaatcata agcttgatgc tgtaatctca
    24661 atgcctagtg gtgtgttcaa gccttatgct ggagtttcaa ctgccattct catctttaca
    24721 aaaactggta atggtggtac tgacaaagtc tggttttacg atatgaaagc ggatggttta
    24781 agtttggatg ataagcgaca accgattagc gacaatgata ttccagatat tatcgaacgc
    24841 tttcatcatc ttgaaaaaga agcagaacgt cagagaacgg atcaatcttt ctttgttcca
    24901 gttgctgaga taaaggaaaa tgattatgat ttgtctatca ataaatataa agagattgag
    24961 tatgaaaaag ttgagtatga accaacagag gtcatcttaa agaaaatcaa tgatttagaa
    25021 aaagaaattc aagctggctt ggctgaatta gaaaaattac tcaagtaggg aggtggctgt
    25081 atgaaaaaag tgaagttggg ggaagtctta tctctaaaaa aaggcaagaa agccactgta
    25141 cttgctgaac aaacaactct aagccaacgt tatattcaaa tagatgattt aagaaataat
    25201 aataatttaa aattcactga aagtttaaat atgactgaag cactcccaga tgatattctg
    25261 atagcatggg atggagctaa tgcaggaaca gttggttatg gattatcggg agctgttggt
    25321 agtacaatta cggtcttaaa aaagaatgag cgatacaaag aaaaaattat atcagattac
    25381 ttgggagtct ttttggaaag taaatcgcag tatttacgag aacattcaac aggtgcaaca
    25441 attcctcatt taaacaagaa tatattactt gatttacaat tagaattgct aggtatcgaa
    25501 gaacaagaga acattatctg tattcttaat acgattaaag ggcttattac taaaagaaaa
    25561 ttgcagttag atgaactaaa cttgctcg
//
LOCUS       DBEJAQ010000029        24752 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.029, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000029 DBEJAQ010000000
VERSION     DBEJAQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.029"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..1505)
                     /locus_tag="ACL297_001282"
     CDS             complement(<1..1505)
                     /locus_tag="ACL297_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HGR0071542.1"
                     /translation="MIYLTEGNTPLNEAYNDEIVHLGNNTYQLTFRFPTSDTKWELLK
                     EETFLTADDLHGEQDFYIFEVEKQQGYIQVYANQVISLLNNYIVSSIEVDRVSGTRVL
                     SAFAGSITRANPFSFFSDIDDRHTLNIKDKNAMEVLAKGKHSILGQWGGDMVRNGYNL
                     RLLKNGGSENESLFMYKKNLSSYQHKTSTKSLKTRITFKTTVKGEGENAVDHDYMVVI
                     DSPLLGNYSQIYEDVVEVNDQDVTDEASLIEYGKQYFRTSMCDMLEDNLEISVVGQSD
                     VAVQMFDVVSFYHEWYGLDVRKKITKYTYSPMAKLLKSIGFGTFQSSLANAIGGIVND
                     AVLNESRNLHQIFEERLKKEIANADRAFDAEFSKREKTITDAIELAKAKAEEVKQELS
                     DTINQRFNSFDNGPLKEAKRKAEEALRNAGASSSLAQESKRIGLDSVARLEAFKSQTT
                     SAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTKKELSGASTL"
     gene            complement(1502..2200)
                     /locus_tag="ACL297_001283"
     CDS             complement(1502..2200)
                     /locus_tag="ACL297_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGR0071543.1"
                     /translation="MFYMIINGFNTSTIPHCVVTDFGQVEAAKPRVVEDANLYGANGS
                     YRVLDGGYESYERTFSFYIPKLLDVSTIVEKFQPKDNVLEFSYQLGSVFYADFIGATY
                     SPHGMHAWKLEIKLNMQPFRYQKNVAPLIFTASGNINNPGSVYSEPVIEIEGDGDISL
                     TIGRTTMHLTVRRKVTIDCRHKKQNIYNADGAVQNTLRKRGGFFELAVGNNSLVFTGS
                     VRKVTVRPNWRYIL"
     gene            complement(2200..4665)
                     /locus_tag="ACL297_001284"
     CDS             complement(2200..4665)
                     /locus_tag="ACL297_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PblA"
                     /protein_id="HGR0071544.1"
                     /translation="MATELGKAYVQIIPSAKGISGMIQKEMGGEVASAGVSAGESLGS
                     KMMGAVSGVIAAAGIGKAIGASINEGAALQQSLGGVETLFKDSADKVKGFANEAYKTT
                     GLSANAYMENVTGFSASLLQSLGGDTDKAAETANMAMIDMSDNANKMGTSMESIQLAY
                     QGFAKQNYTMLDNLKLGYGGTKQEMQRLLADAEKLTGVKYDINNLSDVYSAIHTIQEN
                     LDITGTTAREAATTFTGSFESMKSAAQNVLGKLSLGEDIQPALQALMETTSTFLFGNL
                     IPMIGNILKQIPILILGGIKGVFSGIFGEGLGSIMGSIVTALGSAFLAFKAFSTVSGL
                     LSGIPAVLTTIKTAVTGLFTVMSANPIGIAIAAIAGLTAGLVYFFTQTETGRQIWSSF
                     VAWIKQAWQGIADFFVNLWSGISEGASTLWDGVVTAWNAVVTFFSDLWVRIQEAASVA
                     WTAITTAVMAIVQPFIDGFMNIWNNISDGLTQIWEGIKMIFQGVWEFIKSIFLGAILI
                     IINLVTGNFNQLGADLSLIWKGIKNSISMIWEGIKTYFSGVVDVIVGYGIVIFENFST
                     TLSTIWKGLSAAGKAIFDSFAQILSNIWNTIKSVASSAWEGLKSTVLGLIDGLVQGAK
                     NAWESMKQGVRDLVSNVTSIFDGIRNIDLWSAGKAILDGFLGGLKSSWGAVTDFVGGI
                     ASWIRDHKGPIEYDRKLLIPAGNAIMQGLDRGLQDRFKDVKKSVSGMAGEISNAFSND
                     DFGLSGTPTIAKNIEASLAMPSAQIEAKDSQTVSEIAILRASMEKILTAILEKPSDTY
                     LDADKISMSVYQRQGAIYAREGM"
     gene            complement(4665..5051)
                     /locus_tag="ACL297_001285"
     CDS             complement(4665..5051)
                     /locus_tag="ACL297_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5361 domain-containing protein"
                     /protein_id="HGR0071545.1"
                     /translation="MIALDEDAFICDLAETYQIYDYKQLPLNQVAVFAYGLRDDSRIK
                     QMMSDQIVPLETTLLASIVDRLSLSLWLKTKDGQKGVNRPASIAELLTKNNKEEGDER
                     DYLVFESGEDFENYRKALLAKTGGED"
     gene            complement(5063..5338)
                     /locus_tag="ACL297_001286"
     CDS             complement(5063..5338)
                     /locus_tag="ACL297_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071546.1"
                     /translation="MEEILKGKTESGFEYKIPKKRLRNFYLMREASKMEKGDFEAAEK
                     LLNLLFGKKQAEEFLSHLDDGDDFIDTEVLFADIKSIFESNKDLKKS"
     gene            complement(5344..5901)
                     /locus_tag="ACL297_001287"
     CDS             complement(5344..5901)
                     /locus_tag="ACL297_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGR0071547.1"
                     /translation="MTTASASNVTAAKPKIGGAVSTAPAGTTLPTNAKTDLDAAFETL
                     GYISDDGLTNANSPESKAIKAWGGQTVLSSQTEKKDTFKYKLIEGLNIEVLKEVYGPD
                     NVSGTLETGITVKANGKELPEHCLVIDTLLKNGYVKRVVIPRGKVSEIGEISYKDGEP
                     IGYELTITALPDNSENTHYEYIQGA"
     gene            complement(5968..6303)
                     /locus_tag="ACL297_001288"
     CDS             complement(5968..6303)
                     /locus_tag="ACL297_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071548.1"
                     /translation="MIELVIKKFLDGQLDVPSFFEHKPNMPESYVILEKTGSGGSDYV
                     HSATFAFQSYAPSLQKAAELNEKVKKVVEDLITVNEVSGVHHNSDYNFTDTETKQYRY
                     QAVYDINYF"
     gene            complement(6300..6536)
                     /locus_tag="ACL297_001289"
     CDS             complement(6300..6536)
                     /locus_tag="ACL297_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071549.1"
                     /translation="MSDMKFQLNSAGVSALLRSSEMQGILREKGQGIAERAGEGFELT
                     VSPGQKRANAKISTTDIKSMARNKKHNILLKAMR"
     gene            complement(6529..6867)
                     /locus_tag="ACL297_001290"
     CDS             complement(6529..6867)
                     /locus_tag="ACL297_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071550.1"
                     /translation="MTYLKGIPVILIDKVETGNDDFGHPIHRDVEIEVQNVLVVPTSS
                     EDVINQMNLTGKKAEYTLGIPKGDTNKWENREVKFFGRKWRTIGIPQEGIESMIPLSW
                     NRKVMVEVYE"
     gene            complement(6812..7252)
                     /locus_tag="ACL297_001291"
     CDS             complement(6812..7252)
                     /locus_tag="ACL297_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp19/Gp15/Gp42 family protein"
                     /protein_id="HGR0071551.1"
                     /translation="MGNFAKIEDLELLWRSLKVDERARAEALLKVVSNSLRVEAEKVG
                     KDLDDMVAESVSFASVAKSVTVDIVARTLMTSTDHEPMTQVAESALGYSFSGSYLVPG
                     GGLFIKDTELKRLGLKKKQRYGAIEIYDLPKRNPCHFNRQGGNW"
     gene            complement(7256..7468)
                     /locus_tag="ACL297_001292"
     CDS             complement(7256..7468)
                     /locus_tag="ACL297_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HeH/LEM domain-containing protein"
                     /protein_id="HGR0071552.1"
                     /translation="MTLYRDTKTGVIISAESILGGDWVPVEDKAPSGADLTVAELKSS
                     LDELGIDYDKSSKKSDLVALYEENKG"
     gene            complement(7473..8369)
                     /locus_tag="ACL297_001293"
     CDS             complement(7473..8369)
                     /locus_tag="ACL297_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGR0071553.1"
                     /translation="MADNAMKAGTLFKPELVTELISKVQGKSVLAKLSAQTPIPFNGV
                     EQFIFNLEGNAQIVGEGEQKLGNKAKLTSKIIKPLKFVYQARITDEFQYASEEKRMNF
                     LSAYMDGFAKKIAEAFDLAALHGLEPKTMTDASFRATNSFDGVISGSTVTYDEAKIDE
                     NIEDAVQQVIARGCEVTGIALSPTAGQALAKVKVKDVVQYPEFRFGQNPDSFYGMKSD
                     INKNLTVTGGSAETDHAIVGDFQNRFKWGYAENIPMEIIEYGDPDGAGRDLKAYNEIC
                     LRAEAFIGWGILDEDAFARVKA"
     gene            complement(8375..8839)
                     /locus_tag="ACL297_001294"
     CDS             complement(8375..8839)
                     /locus_tag="ACL297_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HGR0071554.1"
                     /translation="MSEFKTIETQEELDNIVKERIRREREKFGDYDELKKRVSELESE
                     NSALKSTVEDDKQTRAGLDAQITELQGQVSNYETASLRTRIALQNGLPYDLADRLQGA
                     DEEALKADAERLAGFMRPATPPAPLRDTEPAIGDDKTTQMKQMLRELQPKGE"
     gene            complement(8922..10334)
                     /locus_tag="ACL297_001295"
     CDS             complement(8922..10334)
                     /locus_tag="ACL297_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGR0071555.1"
                     /translation="MARKKLGNQNPTQSVILKYVKKNSKAKEAVEIYERTGLSCYAWQ
                     VNLLTSIMAVDKNGLWVHQKFGYSIPRRNGKSELLYLFELWGLHNGLNILHTAHRIST
                     SHSSFEKVKCYLEKMGYVDGEDFSSIRAKGQERIELFDGGGIVQFRTRTSNGGLGEGF
                     DLLVIDEAQEYTTEQESALKYTVTDSSNPITIMCGTPPTPVSNGTVFTNYRKNCLFGK
                     GKYSGWAEWSVSEEKEIDDVDAWYNSNPSMGYHLNERKIEAELGDDKLDHNVQRLGYW
                     PEYNQKSAISETEWNELCVDSMPDLSGKLFVGVKYGQDGANVALSIAVRTVDERIFVE
                     TIDCQSVRNGNDWILDFVKRADVATIVVDGASGQKILDEELKKERMKSVILPTVKEII
                     VANSMWEQGIYQKTLCHAGQPSLKKITTNCEKRNIGSNGGFGYRSHFADMDISLMDSA
                     LLAHWACVTTKPKKKQKISY"
     gene            complement(10385..10615)
                     /locus_tag="ACL297_001296"
     CDS             complement(10385..10615)
                     /locus_tag="ACL297_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071556.1"
                     /translation="MGNTIYFLEKKSSLERGASVKEILEENLEASHDYTSVLVVSLDK
                     DGEINLGYSWDSSLQALGMLDVAKNYILNVIN"
     gene            complement(10668..10907)
                     /locus_tag="ACL297_001297"
     CDS             complement(10668..10907)
                     /locus_tag="ACL297_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071557.1"
                     /translation="MKSDYEEVNWSEYCYKENDDDKTWWVDTSWYAKGLMLITFDKKK
                     FYNLFEDYPQNMTSEEIEIFDKENPFWEDFFSDRK"
     gene            complement(10897..12288)
                     /locus_tag="ACL297_001298"
     CDS             complement(10897..12288)
                     /locus_tag="ACL297_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071558.1"
                     /translation="MVEDIVPSLLKKIKSEFEGARLDSEVLKDLLSKLQHSKASYLDA
                     NQYAIEIGEILSKALGASLTNETLPDGKMYYNIAQRVLTDVLGRNYELVSDYTEQVQK
                     NLNSEAKIGLTAQVPELNQDRIDGLVNRLASEESFDDVRWLLEEPVVNFTQSIIDDSI
                     QKNAEFQHKSGLQPEIVRKSAYHCCEWCQEVQGTYKYPRVPKDIYRRHQRCRCTVDYD
                     PKSGKVQNVWSKAWSKSDKSDKIEARKNIGIQSEVSQVRKLALQIGITSNPITKSLKK
                     LTEEEIIQAISGGDKTKGSCSSLAFAYIGNKAGYTVLDFRGGKSCVFFGSIDKINMIG
                     SLPGVKMHVAKNTNDFKAVKELLEKAENGKEYYLATGRHAAIIRKNDNLVEYLELQSK
                     FVNGFKPFDDTVLKKRFKAKKSHAVRGHKYDVDSYLIDANSLKDNPEFHNILSFLNTA
                     ESKQMKGITGYEK"
     gene            complement(12281..13744)
                     /locus_tag="ACL297_001299"
     CDS             complement(12281..13744)
                     /locus_tag="ACL297_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071559.1"
                     /translation="MTLKGMGYLRKKLANYKMGVDTRYKQYAMQYNDIDVGITIPPQI
                     RQQYRAVLGWAAKGVDSLADRLVFREFANDEFGANEIFAQNNPDVFFDSAILSALIGS
                     CCFVYISQGDDDDAPRLQVIEASNATGVLDPITGLLTEGYAVLKRDDNGYAVLEAYFT
                     SDVTWFYPKDGKPFAIGNPTGVPLLVPVIHRPDAVRPFGRSRITRAGMYYQRYAKRTL
                     ERSDVTAEFYSFPQKYVLGLSQDAEAIDTWKATVSSLLTFTKDDEGDKPNVGQFTTSS
                     MSPFTEQLRTAAAGFAGEMGLTLDDLGFVSDNPSSVEAIKASHENLRLAGRKAQRSLG
                     SGLLNVAYVATCLRDEFPYLRKQFNKTVVKWEPLFEADANMLTLIGDGVIKLNQAVPG
                     YMDAETIRDLTGIKGDMNATPKIEEVEQKSTNSEDKQKNRIISTYEITSLLSNYQKGV
                     LSKENGILLLTSTGMSKQEAEAMINKTELIVKEAADG"
     gene            complement(13882..14226)
                     /locus_tag="ACL297_001300"
     CDS             complement(13882..14226)
                     /locus_tag="ACL297_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGR0071560.1"
                     /translation="MNTNPLRADRSGPHRVAFEKNKKIILKTRNTCGICGQPVDKDLR
                     YPHPLSPVIDHIVPVNKNGHPSDIANLQLAHWQCNRQKSDKLYADEKTNGTKVIGNRN
                     LPQSTDWFKYKG"
     gene            complement(14460..15026)
                     /locus_tag="ACL297_001301"
     CDS             complement(14460..15026)
                     /locus_tag="ACL297_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGR0071561.1"
                     /translation="MKKVEPIRDLDDIERIKDYLKNKSDRNYVLFMFGIYSGLRVSDI
                     VPLQVKQVIADRIELKEKKTGKIRYFPISPPLRKEINRYIKDNQLAEYDYLFPSKKKK
                     RTDGVRITHIGRVAVYQILQDAAKYVGLNHIGTHSMRKTFGYHHYKKNSNVAILQKIF
                     NHSTPDITLGYIGYSQDELDQSILSFDY"
     gene            complement(15316..15744)
                     /locus_tag="ACL297_001302"
     CDS             complement(15316..15744)
                     /locus_tag="ACL297_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HGR0071562.1"
                     /translation="MQIELLDIIDEKKTRKEAIKVLKKYSRLRRIAGEEYAPKITISY
                     SLEPRSSSGQTSKQVESMVVRRVSAQQDLELIAKAINNLSDMEYTRILIERYCRKKRR
                     EDYSIYSELGYSSSEYYRILNKALLEFAESYQASNLLVYK"
     gene            complement(15817..16050)
                     /locus_tag="ACL297_001303"
     CDS             complement(15817..16050)
                     /locus_tag="ACL297_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0071563.1"
                     /translation="MMNDKLNPRQASRFAFLLKQKRKERKLSQEKLAAKLGYSRYLIA
                     QWEKEESVPDIYNVEDICTYFAFPADIILGSRA"
     gene            complement(16040..16216)
                     /locus_tag="ACL297_001304"
     CDS             complement(16040..16216)
                     /locus_tag="ACL297_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071564.1"
                     /translation="MSEYALYQGDVFITLGTLAEISKETGIAERMLKYYTFASTQRRN
                     PNGRAVVKIEVDDE"
     gene            complement(16209..16442)
                     /locus_tag="ACL297_001305"
     CDS             complement(16209..16442)
                     /locus_tag="ACL297_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HGR0071565.1"
                     /translation="MNPEIIDNINKPSHYQGRFGMESIDTLRNFLTPEQLKGFYMGNS
                     LKYILRHQKKNGLEDLKKARKNLDWLIEEMEHE"
     gene            complement(16439..16891)
                     /locus_tag="ACL297_001306"
     CDS             complement(16439..16891)
                     /locus_tag="ACL297_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HGR0071566.1"
                     /translation="MWHHELGRLMSVKCMFFQDSEIEEFELNDTLMNDYITAYPDEIE
                     LMQSTGLFDRNGKEIFEGDILKFNDEWNEYCHEGYVDGSVEGVNYVEVVRSEICFEFG
                     KTKYPDSSLFILMEDEQLNFKDFIKSEDFEFEIIGNIYENPELLEDKE"
     gene            complement(16938..17276)
                     /locus_tag="ACL297_001307"
     CDS             complement(16938..17276)
                     /locus_tag="ACL297_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071567.1"
                     /translation="MSYDLEILAKIESGDYICIAEPKNSSPTYNLGRMFRVAMNWDFD
                     QGTIYNVADIFENIKRGITELERQPEKYVQYEPVNKWGTVNAALYVLRSLRDCILEQD
                     IDTKYLYVRW"
     gene            complement(17276..17563)
                     /locus_tag="ACL297_001308"
     CDS             complement(17276..17563)
                     /locus_tag="ACL297_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071568.1"
                     /translation="MTQTLEEGMKNQSKCIKIPMEIRPFDVGYRIVNKHGQALALKNG
                     ASIFALPFLAEKAIEKEFGKNDPDFDIGKHSVEEVAIVNLSKFHSYFEEVE"
     gene            complement(17563..18078)
                     /locus_tag="ACL297_001309"
     CDS             complement(17563..18078)
                     /locus_tag="ACL297_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HGR0071569.1"
                     /translation="MNIKALIKKYEELWNERSPFYEPVPYTSMVELFLKELKQLDEPE
                     KVKVPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLNGYE
                     VEKEKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEEAGLGWVF
                     DCEGIEIEEVE"
     gene            complement(18080..18397)
                     /locus_tag="ACL297_001310"
     CDS             complement(18080..18397)
                     /locus_tag="ACL297_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071570.1"
                     /translation="MMEELKQKVNAVYNWTVEDGKPQPPKQDLPQVVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWIGYAPSMAQVVIAVYLIY
                     GGN"
     gene            complement(18369..18578)
                     /locus_tag="ACL297_001311"
     CDS             complement(18369..18578)
                     /locus_tag="ACL297_001311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071571.1"
                     /translation="MKYDKQAEIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LRKKNKELRRKLEELEDDGRVKAKS"
     gene            complement(18565..18732)
                     /locus_tag="ACL297_001312"
     CDS             complement(18565..18732)
                     /locus_tag="ACL297_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071572.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            complement(18859..19086)
                     /locus_tag="ACL297_001313"
     CDS             complement(18859..19086)
                     /locus_tag="ACL297_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071573.1"
                     /translation="MLELYFVYNGHCKFFLGRFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            complement(19086..19280)
                     /locus_tag="ACL297_001314"
     CDS             complement(19086..19280)
                     /locus_tag="ACL297_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071574.1"
                     /translation="MFILKHGTREDKPFLRSAIISVTGLDISCSEEKKAMRFISRAAA
                     LQVSKALRGSFGNFYPVEVE"
     gene            complement(19295..20065)
                     /locus_tag="ACL297_001315"
     CDS             complement(19295..20065)
                     /locus_tag="ACL297_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGR0071575.1"
                     /translation="MVTDALEEMALSYHRNTEQQTEICEKHNIPFIKILRTESVVCRM
                     CESERIHEENQARVNELANAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LAFAKRICREWYEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            complement(20059..20217)
                     /locus_tag="ACL297_001316"
     CDS             complement(20059..20217)
                     /locus_tag="ACL297_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071576.1"
                     /translation="MGVLELIKQFEEEFYPISEEKKSLLIKQPLSTVITCLSDMASWK
                     ACGGKVSW"
     gene            complement(20205..21026)
                     /locus_tag="ACL297_001317"
     CDS             complement(20205..21026)
                     /locus_tag="ACL297_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HGR0071577.1"
                     /translation="MALKNKRYFWIQLAQDFFKSKEMKLLRKIAGGDTHTIIYLKMML
                     ISLEDGGHIYYDGLADNLAEEIALVIDENVEDIKITLIFLESKGLLTRNSDRDYFLEQ
                     VPEMVGSETASARRVRKFRENKLALQCNTDETKCNGDIDIDTDKEIDKDIDKDIEKES
                     NKTMVSSSLSENLKHSGIRINNKQHQQLLEYVGLDGMSFDMLNRAIEITSEVYRPSFK
                     YLRGVLENWKKKGFTTIEQVDDNDQKYKDSKNYNRSGRQRNDKKSEQEVKNEWGF"
     gene            complement(21017..21265)
                     /locus_tag="ACL297_001318"
     CDS             complement(21017..21265)
                     /locus_tag="ACL297_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071578.1"
                     /translation="MLSSDEEKSDFELYLEDENRKISLIKEYLTDYGKLAKDYRDVVS
                     ENKLLKLEKMELEGRRIHEDMRMKYRANRRKWGVRLWR"
     gene            complement(21329..21652)
                     /locus_tag="ACL297_001319"
     CDS             complement(21329..21652)
                     /locus_tag="ACL297_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071579.1"
                     /translation="MFEPPILDQLMGVGALLIGFAGLCRHIKLQEERKEKERREEQEF
                     ASMIIQGRNYAYERGREDKWQEIRKNIRREFKGFTYDNEPPVGLRPEPLALPEPKQSA
                     IRFLS"
     gene            complement(21738..21995)
                     /locus_tag="ACL297_001320"
     CDS             complement(21738..21995)
                     /locus_tag="ACL297_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGR0071580.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCE
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            complement(22008..22718)
                     /locus_tag="ACL297_001321"
     CDS             complement(22008..22718)
                     /locus_tag="ACL297_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HGR0071581.1"
                     /translation="MNEIFNFHGQEVRTVMFDDEPWFVGKDIAEILGYVNSRDALAKH
                     VDEDDKLTSQIATAGQMRNQTVINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRKQ
                     GGFIREDLDEDAFIALFTGQKKLREQQTSMLEDIDYLKSEQPIHPSYAQSLLKKRKAR
                     VVACLGGIDSPAYADKIFAQSVFRQAEIDFKDHFNISRYDLLPKKHADAALAYWMTWE
                     PSTNTKMKIMKLNSFDEG"
     gene            complement(22746..22961)
                     /locus_tag="ACL297_001322"
     CDS             complement(22746..22961)
                     /locus_tag="ACL297_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0071582.1"
                     /translation="MVNVSKLKGKIIERNTTQEALAKDIGIDKSTFYRKMKQNGSFSI
                     QEVNLIVSSLGLSKDEALSIFFSETVA"
     gene            23127..23861
                     /locus_tag="ACL297_001323"
     CDS             23127..23861
                     /locus_tag="ACL297_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0071583.1"
                     /translation="MNVGERIKQRRKALKMSADELAESVGVSRSTIFRYEKGDIEKVG
                     PEVLKKIADKLNVSPGDLMGWEDNQQELKIPTSPLVHKITEKVVKLSTPRKQKVLNYA
                     NEQLKEQNNKVITIEEKLFEYRVFEKLSAGTGFSYFNDGNYDTVFYDKDLDHDFASWV
                     FGDSMEPKYMNGEVVLIKETGFDYDGAIYAVDWDGQTYIKKVYKEKDGLRLVSINNKY
                     KDKFAPFEEDPRIIGKIVGNFMPIEN"
     gene            23874..>24752
                     /locus_tag="ACL297_001324"
     CDS             23874..>24752
                     /locus_tag="ACL297_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HGR0071584.1"
                     /translation="MKIGMRKPSLTRSLKARTTSKWKRQAKKALIPGYGKKGMGWVKN
                     PKKAMYNKVYHKTTFGLSDLFKSSKKSKKKVDTNKQQSILSSSGKKQHTPKEYKEAGL
                     VFMVLGAIFLFLFPPLGFFLFITGFITYIIGRLTAKREKKQKVENYSPQIDTIVFRDN
                     FLLMGTNYHQEEAEIAADFLSKGVHYFGKDNKSLKSYMLETYKPVYKYNKLKTVDVQL
                     LPEPSNPHDKNAVKVLVNNIFVGYLPAVIASQISSYIANPNYRYDAILTGRGGPYKTL
                     NIETERVVTRKKELTYY"
BASE COUNT         6967 a         5792 c         4107 g         7886 t
ORIGIN      
        1 agggtacttg cgccagacag ttcttttttg gtctgaacaa gtgcttcaac ttgcttggca
       61 atctcagctt caacctgtgc ttgcttcggt cgaatatcat tcacgatagt ccgtttcaga
      121 gcgtccaagt cacctgacag agccgtttgt gcgctcgtag tctgcgactt aaacgcttca
      181 agtctagcaa cagaatccag cccaatccgc ttggattcct gagcaagaga actacttgcg
      241 ccagcatttc gcaaagcttc ctcagccttg cgcttagctt ctttcaatgg cccgttgtca
      301 aagctattaa agcgctgatt gatagtgtca gacagttctt gcttgacttc ttccgctttg
      361 gccttggcaa gttcgatggc atccgtgatg gttttctcac gcttggaaaa ttcagcgtca
      421 aaggcacggt cagcgttggc gatttccttt ttcaaacgtt cttcaaaaat ctgatgcaga
      481 tttcggcttt cattcaaaac ggcatcattt acaatcccac cgatcgcatt tgcaagactg
      541 gactggaagg ttccaaaacc tattgatttt aggagttttg ccattggcga ataggtatat
      601 ttggtgattt tcttacgaac atcaagaccg taccactcat ggtagaaact gaccacatca
      661 aacatctgca ccgcaacatc actctgaccg acaaccgaga tttcaaggtt atcttctagc
      721 atgtcgcaca tacttgtccg aaaatactgc ttaccgtatt caatcaagct agcctcatct
      781 gtcacgtctt ggtcattgac ttccaccaca tcttcgtaga tttggctgta gttcccaagt
      841 aaggggctgt caatcaccac catataatca tggtcaaccg cattttctcc ctcgccctta
      901 acagtcgttt taaaggttat ccgagttttt aaagacttcg ttgaggtctt atgctggtag
      961 ctggacaagt ttttcttgta cataaaaagc gattcatttt cagaaccgcc attcttcaac
     1021 aagcgtaaat tgtagccgtt tcgcaccata tctccgcccc actgaccaag gatagaatgc
     1081 ttgcctttgg ccaagacctc catggcattc ttatccttga tattgagcgt atgcctatca
     1141 tcaatatcag agaagaaaga aaaaggattg gctctggtaa tactaccagc aaaagcactc
     1201 aacacccttg tcccactgac acggtcaacc tcgatagagc taacgatgta attatttaac
     1261 aaactgataa cctgattggc atagacttga atatatcctt gttgcttttc aacctcaaaa
     1321 atataaaaat cctgctcacc atgcaggtca tctgcagtca gaaaagtttc ctctttcagt
     1381 aattcccact tggtatccga tgtaggaaaa cgaaaggtca gttgataggt attgttcccc
     1441 aaatggacaa tttcatcatt gtaggcctca tttaaaggtg tattgccttc agtaagataa
     1501 atcataagat atacctccaa ttcggccgaa ccgtgacctt gcgaaccgaa ccagtaaaga
     1561 ccagactgtt attaccaact gccaactcaa agaagcctcc acgtttacgt aaagtatttt
     1621 gaaccgcgcc atctgcatta tagatattct gtttcttatg cctacaatca atggtcactt
     1681 ttcgtctaac ggtcaagtgc atagttgtcc ttccgatagt caaagaaata tcaccgtctc
     1741 cctcaatctc aatcacaggc tcgctataga cagagcctgg attgttgata tttccacttg
     1801 cggtaaagat aagaggagca acatttttct gataacggaa cggttgcatg ttcaacttga
     1861 tttctagttt ccaggcatgc atcccatgag ggctataggt tgcaccaata aaatccgcat
     1921 aaaatacaga ccccaactgg tagctaaact ctagcacatt gtccttaggc tgaaatttct
     1981 ccacgatagt agaaacatcc agtaacttag gaatatagaa cgaaaaagtc cgttcataac
     2041 tctcatagcc accatccagc actcgataac ttccgttggc tccgtagagg ttagcatctt
     2101 caaccaccct aggtttagct gcctccacct gaccaaaatc cgtcaccacg cagtgaggga
     2161 tagttgatgt attaaaccca ttgataatca tataaaacat tacattccct ccctagcata
     2221 aatcgcacct tgacgttggt agacgctcat tgaaatttta tcagcgtcta ggtaagtatc
     2281 tgacggcttt tcaaggatag cagtaaggat tttctccata cttgctctca gaatcgctat
     2341 ctcagacacg gtttgactgt cttttgcctc gatttgagcg cttggcatgg ccaaacttgc
     2401 ttcaatattt ttggcaatag tcggtgttcc actcaagcca aaatcatcgt ttgaaaatgc
     2461 gtttgaaatt tcgccagcca ttccactgac agatttctta acatctttga aacggtcctg
     2521 caaccctcta tccaaacctt gcataatcgc attaccagca ggaatcaaga gcttgcggtc
     2581 atactcaatc ggacctttgt ggtcacgaat ccagctagca ataccaccaa cgaagtcagt
     2641 tactgctccc caagaagact tcaaaccgcc taggaatcca tcaaggatag ccttacctgc
     2701 tgaccatagg tcaatgtttc gaatgccatc aaagatactc gtaacattac ttacaaggtc
     2761 acgaacacct tgtttcatgc tttcccatgc atttttagct ccttggacaa gtccgtcaat
     2821 cagacctaag acggttgatt tcaacccttc ccaagcactg cttgctacag atttgattgt
     2881 attccagatg ttagataata tctgagcaaa actatcaaag atagctttac ctgcagcaga
     2941 caaccctttc caaattgtac taagagtggt agaaaagttt tcaaaaataa caataccata
     3001 acctacgata acatccacga ctccagagaa gtatgtttta atcccttccc aaatcataga
     3061 gatactattt ttaatccctt tccagattag agaaagatca gcccccagct ggttaaagtt
     3121 ccctgtcaca aggttgatga tgatgagaat agcacccaag aaaatagact tgatgaattc
     3181 ccaaacgcct tggaaaatca tcttaatccc ttcccaaatt tgggtaagac catctgaaat
     3241 attgttccaa atattcatga atccatcaat gaacggttga acaatagcca tcactgctgt
     3301 tgtgatagct gtccatgcca cagatgcagc ttcttgaatc cttacccaca agtcagaaaa
     3361 gaatgttaca acagcattcc aggctgtaac aactccatcc cacaatgtgc tagcaccttc
     3421 agagatgcca gaccaaaggt ttacaaagaa atcagcaatc ccctgccaag cctgtttgat
     3481 ccaagctaca aaagatgacc agatttgtct acctgtttca gtttgagtga agaaataaac
     3541 caaacctgca gttagtcctg caatcgctgc gatggcaatt ccaataggat tggcactcat
     3601 tacagtaaat agacccgtga ctgctgtttt aattgtcgtt aagacagcag gtattccaga
     3661 tagcaatccc gagactgtcg aaaatgcttt aaacgctaaa aatgcagaac caagagcggt
     3721 aacgatacta cccatgatac ttcctagacc ttcaccaaag attccactga aaacaccctt
     3781 tatccctccc aaaataagga taggaatttg tttcagaata tttccaatca ttggaattag
     3841 gtttccgaaa agaaatgttg atgtcgtttc catcaaagct tgtagtgcag gttgaatatc
     3901 ttcacctaaa gacaactttc caagcacatt ctgagcagct gatttcatcg attcaaatga
     3961 acctgtaaat gtagttgctg cctctctagc tgtcgtacca gtgatgtcca aattctcttg
     4021 tatagtgtga atggcgctat aaacgtctga tagattattg atgtcatatt taacacctgt
     4081 caatttttca gcatccgcca aaagtcgttg catttcttgc ttcgtaccac cataacccaa
     4141 tttaaggttg tctagcatcg tataattttg cttggcaaaa ccttgataag ccagttgaat
     4201 actttccata gatgtcccca tcttattggc attatccgac atatcaatca tggccatgtt
     4261 agctgtttct gctgctttat ctgtatctcc accaagagat tgtagcaagc ttgctgagaa
     4321 acctgtaaca ttttccatat aggcattggc tgagagacct gttgtcttgt aagcctcgtt
     4381 cgcaaatcct ttgaccttat cagctgagtc tttaaataag gtttcaaccc ctccaagaga
     4441 ttgttgaagt gctgcccctt cgtttatcga tgctccgatt gctttaccaa ttccagctgc
     4501 agcaataact cctgaaacag cccccatcat tttagatccg agggattcgc ctgcgctaac
     4561 gccagctgag gcaacttcac cacccatttc cttttgaatc atgccactaa tgcctttagc
     4621 agacggaatg atttgtacat aggcttttcc taattcggtc gccactaatc ctcacctcct
     4681 gtttttgcaa gcaaagcctt gcgatagttt tcaaagtcct caccagattc aaagacgaga
     4741 taatcccttt catctccctc ttctttgtta ttctttgtaa gtaattcagc aattgatgca
     4801 ggacgattaa cacccttttg cccatctttt gttttcaacc acaaagaaag agacagtctg
     4861 tctacgatac ttgcaagtaa cgtcgtttca agagggacga tttggtcaga catcatctgc
     4921 tttatccgcg aatcatcacg caacccatac gcaaaaacag ccacctgatt taaaggtagc
     4981 tgtttatagt cgtatatttg gtaggtttcc gctaaatcac agataaaagc atcctcgtct
     5041 aaggcaatca tctgagcaag gactaagatt tttttaggtc tttgttggac tcaaagatac
     5101 tcttgatatc tgcgaacagt acctcagtat cgatgaagtc gtctccatca tctaaatgag
     5161 ataaaaactc ttccgcttgt tttttaccaa agagaagatt cagcaatttt tcagcagctt
     5221 caaaatcccc cttctccatt ttggaagctt cgcgcatgag ataaaagttt ctcaatcgtt
     5281 ttttagggat tttgtactca aaccctgact ctgtttttcc ttttaagatt tcttccattt
     5341 attttacgct ccttgaatgt attcgtagtg agtgttttca ctgttgtctg gtaatgcagt
     5401 gatcgtcaat tcatagccga taggttcgcc gtctttatag ctgatttcgc caatttcgct
     5461 aaccttacca cgaggaatga caacgcgttt cacatagcca tttttcagca aagtatcaat
     5521 aaccaagcaa tgttctggca actctttacc gttggctttt acagtgatac ctgtttcaag
     5581 cgttcctgag acgttatctg gtccatagac ttctttcaag acttcaatgt tcagaccttc
     5641 aattaatttg tatttgaagg tatctttctt ttcagtttga gaagacaaga ctgtttgtcc
     5701 accccacgct ttgattgctt tgctttctgg cgagttcgca ttggtcaatc catcatctga
     5761 aatgtaccct agcgtttcaa atgctgcatc caaatctgtt ttggcattcg ttggaagagt
     5821 tgttccagct ggagcagtag aaactgcccc tccaattttc ggcttagcag ccgttacatt
     5881 tgatgctgat gcagtcgtca tattctttcc tcctgttgat tctgtatttt gtcttcttgc
     5941 ttctgtcgtc aaaactacac ctccttttta aaaataatta atatcatata ccgcttgata
     6001 gcgatattgc ttcgtttccg tgtctgtaaa gttgtagtca ctattgtgat gcacaccgct
     6061 gacttcgttg accgtgatga gatcctcaac tactttcttg actttctcat tcagctcagc
     6121 agccttttga agtgatggtg cataactttg aaaagcgaat gtagcggaat gaacgtagtc
     6181 gcttccacca cttccagtct tttctaaaat gacataactc tcaggcatat tcggtttatg
     6241 ttcaaaaaaa gacggtacat ctaactgtcc gtccaaaaat ttctttataa ctaattcgat
     6301 catctcatag ccttcagtaa aatattatgt tttttatttc tagccatgct cttgatatca
     6361 gtcgtactaa tctttgcatt ggcacgcttt tgccctggcg atacagtcaa ttcaaacccc
     6421 tcaccagctc tttcagcaat cccttgccct ttctccctca aaataccctg catttcggaa
     6481 gaacgtagca aggcagacac cccagccgag ttcaattgaa atttcatatc actcataaac
     6541 ttcaaccata acctttctat tccacgataa tggaatcatt gactcaatcc cctcttgagg
     6601 gatgccaatc gtccgccatt tacgaccaaa aaacttaacc tcacggttct cccacttgtt
     6661 agtgtctcct ttaggaatac caagtgtata ttccgccttt ttcccagtca aattcatttg
     6721 attgatgacg tcctctgatg aagttgggac aaccaataca ttttgaacct caatctcaac
     6781 atcacgatgg attggatgac cgaaatcgtc attaccagtt tccaccttgt ctattaaaat
     6841 gacagggatt ccttttaggt aggtcataaa tttcaatcgc tccatatcgt tgttttttct
     6901 tcaaaccaag ccttttaagt tcggtgtctt tgataaagag accgccacca ggtacaaggt
     6961 aagaaccact aaacgaataa cccaaggcgc tttcagcaac ctgagtcatt ggttcatggt
     7021 ctgttgaggt catgagagtt cgtgccacga tatcgaccgt gacagacttg gcaacactag
     7081 cgaatgacac gctttctgcc accatatcgt caaggtcttt accgactttt tcagcttcca
     7141 ctcgcaaaga attagataca actttcaaca aagcctcagc ccttgcacgc tcatcaactt
     7201 tcaacgaacg ccacaataat tccaagtctt caatctttgc aaaatttccc atagcttaac
     7261 ccttgttttc ctcgtacaag gctaccaaat cggatttttt tgaactctta tcgtaatcaa
     7321 tgcctaattc atccaaacta gacttcaatt ccgctacggt caaatccgct ccgcttggtg
     7381 ccttatcttc cacaggcacc caatctccgc caagaatgct ctcagcagag ataatcacgc
     7441 ccgtttttgt atcacggtat aaagtcataa tcttacgctt tcacacgcgc aaaggcatct
     7501 tcatcaagga taccccaacc aataaaggct tccgcacgca aacagatttc attgtaggct
     7561 ttaaggtcac gaccagcacc gtctggatca ccgtattcaa tgatttccat tggaatattt
     7621 tcagcataac cccacttgaa gcggttttgg aaatccccga caatagcatg gtctgtctcg
     7681 gcactaccac cagtgacagt aaggttcttg ttgatatctg atttcattcc gtagaacgaa
     7741 tctggatttt gaccaaaacg gaactcagga tattgaacga catcctttac tttaactttc
     7801 gccaatgctt gacctgcagt tggtgacaag gcaatacctg ttacctcgca accacgggca
     7861 ataacctgtt gtactgcatc ttcaatattt tcatcaattt ttgcttcatc atacgtcacg
     7921 gtacttccgc taatcacacc atcaaatgag tttgttgcac ggaaagaggc atccgtcatt
     7981 gtttttggtt ccaaaccatg aagagcagca aggtcaaagg cttctgcaat cttcttagca
     8041 aaaccgtcca tataagctga caagaagttc attcgttttt cttctgaagc gtattgaaac
     8101 tcatctgtaa tacgggcttg ataaacaaat ttaagcggtt tgataatttt cgaagtcaac
     8161 ttcgctttat ttccaagttt ttgctcaccc tcaccaacga tttgcgcatt accttcaagg
     8221 ttgaagatga attgctccac cccattaaat ggaattgggg tttgtgccga caatttagcc
     8281 agtacagatt tcccctgtac cttgctaatc aattctgtta ctagttctgg tttaaaaagt
     8341 gttccagctt tcattgcatt atctgccata aattctattc tccttttggt tgtaattcac
     8401 gaagcatctg cttcatttgc gtcgttttgt catcaccgat agcaggctcc gtatctctta
     8461 gtggcgctgg aggtgttgct ggacgcataa agccagctag acgctcagcg tcagccttta
     8521 atgcctcttc gtcagcgcct tgaagacggt cagccaagtc ataaggcaag ccattttgta
     8581 aagcgatacg agttcgcaag ctagcagttt cataattgct cacttgcccc tgcaattcag
     8641 tgatttgagc atctaatcct gctctggttt gcttgtcatc ttcaacagta gacttcaaag
     8701 cactgttttc agattccagt tctgaaacac gtttcttgag ttcatcataa tcaccgaatt
     8761 tttcacgctc acgtctgata cgttccttca cgatgttatc tagctcttcc tgtgtttcaa
     8821 tcgttttaaa ttcagacatc ttcatgtctc ctttctcctg ctttcccggc agttcggtaa
     8881 ttttttaggc atcaaaaaaa gcagtctctc aaccgctcct cttaataact gattttttgc
     8941 tttttcttag gcttagttgt cacacaagcc caatgcgcaa gcaaggcgct atccatcaaa
     9001 gaaatatcca tatccgcaaa atgcgagcga tagccaaacc caccatttga accgatgttc
     9061 cgcttctcgc agttggttgt gattttcttc aaagacggtt gaccagcatg gcacaaggtc
     9121 ttttgataaa tcccttgttc ccacatcgag ttagccacga tgatttcctt aaccgtaggc
     9181 aatatcacgc tcttcatgcg ttcctttttc aactcttcat caaggatttt ctgaccgctt
     9241 gctccatcga ctacgatagt agccacatcg gcacgcttga caaaatccaa gatccagtca
     9301 ttcccgttac ggactgactg acagtcaatt gtctcaacga aaatccgttc atctacagta
     9361 cgaacagcaa tacttaatgc cacgtttgcg ccatcttgac catatttgac tccgacaaac
     9421 aacttacctg ataaatcagg catagagtca acacacaact cattccattc cgtttccgaa
     9481 atagcagatt tctggttgta ttcaggccaa taacccaaac gctgaacatt atggtctagc
     9541 ttatcatcac caagctcagc ttctatcttc cgctcattca aatggtaacc catagaggga
     9601 ttggaattat accaggcatc gacatcatcg atttcttttt cctcagaaac cgaccattct
     9661 gcccaaccag agtattttcc tttcccaaaa aggcaattct tacggtaatt tgtgaatacc
     9721 gtcccatttg aaacaggtgt aggaggtgtc ccacacatga ttgtaattgg attgctacta
     9781 tccgttaccg tatatttcaa agccgattcc tgctcagtcg tatattcctg agcctcatcg
     9841 ataacgagaa ggtcaaaacc ttcccccaaa ccaccattgg atgttctggt acgaaattgt
     9901 acaatcccac cgccgtcaaa cagttcaatc cgctcttgtc ccttggctcg tatagagcta
     9961 aagtcctcac cgtccacata tcccattttt tcaaggtaac atttcacctt ttcaaaagag
    10021 gaatgagagg tggatattct atgagccgtg tgtaggatgt ttagtccatt atgcaggccc
    10081 caaagttcaa aaagatacaa gagttcggac ttcccattac gacgaggaat agagtagcca
    10141 aatttttgat gcacccacaa accattcttg tcaaccgcca tgatagaggt caacaagttg
    10201 acttgccaag cgtagcaaga aagacccgtc cgctcgtaga tttctaccgc ttctttcgcc
    10261 ttagaatttt tcttgacgta ctttaaaatt accgattgag taggattctg attgccaagt
    10321 ttctttctag ccatccactg ctcctttcaa tcgtaccgca tgataaccct atcgctggga
    10381 tgatttaatt gattacgttt aaaatatagt ttttagcaac atctagcatt cccaatgcct
    10441 gcaaactact atcccagcta tagccaagat ttatctcacc atctttatcc aaagaaacta
    10501 ccaacaccga agtgtagtca tgactagcct caagattttc ctccaaaatt tctttcacgg
    10561 aagcaccgcg ctccagacta gactttttct ctaaaaaata aatcgtgttt cccattgtta
    10621 ctcctttcta agcataagaa aagcaccctt gcgagtgctc taacatatta ttttctatct
    10681 gaaaagaaat cttcccaaaa tggattttct ttatcaaaga tttcaatctc ctcagaagtc
    10741 atgttttgag gataatcttc aaaaaggtta tagaacttct ttttatcaaa tgtgataagc
    10801 atcaatcctt tggcatacca tgaagtatca acccaccaag ttttatcatc atcgttttcc
    10861 ttgtaacaat actctgacca attcacttct tcgtaatcac ttttcatatc ctgtaatacc
    10921 tttcatttgt ttagattcag ctgtgttaag gaaactcaat atgttatgaa attcagggtt
    10981 gtcttttaat gaatttgcat caataagata gctatctaca tcatatttat gtccccttac
    11041 agcatgagat ttcttagcct taaatctttt tttcaaaaca gtgtcatcaa atggcttaaa
    11101 cccatttaca aattttgatt gaagctccaa atattcaact agattatcat ttttcctaat
    11161 gattgcagca tgtctacctg ttgccaaata gtattctttg ccattttctg ccttttccaa
    11221 caattctttg accgctttga agtcatttgt gtttttggct acatgcatct taacacctgg
    11281 aaggcttcca atcatattga ttttatcaat actaccaaaa aaaacgcaac tctttccacc
    11341 tctaaaatct agaactgtat atcccgcttt atttcctatg taagcaaatg ctaaggatga
    11401 acaagaccct ttcgtcttat ctccaccact aatcgcttga ataatttctt cttcggttaa
    11461 ttttttaagg cttttcgtta tagggtttga agttatacct atttgaagag caagctttct
    11521 aacctgactg acttcagatt gtattccgat gttctttctt gcttctattt tatcactttt
    11581 atcactttta ctccacgcct tactccaaac attttggacc tttccacttt taggatcata
    11641 atcaacagtg catctacaac gttgatgtct tcgataaata tcctttggaa ctcttgggta
    11701 tttgtaagta ccttgaacct cctgacacca ttcacaacag tgataagctg attttcttac
    11761 aatctcaggt tgcaatccag atttatgttg aaactccgca tttttctgga tgctatcatc
    11821 aattattgat tgagtaaaat tcacaacagg ttcttctaat agccaacgaa catcatcaaa
    11881 actttcctca ctggctaaac gattaaccag accatcaatt cggtcttgat tcaattcagg
    11941 aacctgagca gttaacccaa ttttagcctc agagttcaaa ttcttctgaa cttgctcagt
    12001 ataatcactc acaagctcgt aatttcgccc cagaacgtcc gtcagcacac gttgagcgat
    12061 attgtaatac attttaccgt ctggtagcgt ttcgttcgtt agagaggctc ccagagcctt
    12121 agaaagtatc tccccaattt caatagcata ttgattagcg tccaaataac ttgccttgct
    12181 gtgttgtagt ttagacagca agtctttcaa gacctcgctg tctagcctag caccttcaaa
    12241 ctcagacttg attttcttga gcaggctcgg aacgatatcc tcaaccatct gcagcctcct
    12301 tcacaattaa ttcagtctta ttaatcatag cttccgcttc ttgcttactc attccagttg
    12361 aggttagtaa gagtattccg ttttcttttg aaagtacccc tttttgatag ttactcagta
    12421 gagaggtaat ctcatatgta gaaatgattc tatttttttg cttatcctct gagttagtac
    12481 ttttttgttc tacttcctca attttcggag tagcgttcat atcgcctttg attccagtca
    12541 agtcacggat ggtctcagca tccatatatc caggcaccgc ttgattcagt ttgataacac
    12601 catcaccaat caaggtcaac atgttagcgt ccgcctcaaa caaaggctcc cacttcacga
    12661 ccgttttatt gaactgtttc ctcaaatacg gaaactcatc acgtaaacaa gtagccacat
    12721 aagccacatt cagcaaacca gagcccaaag agcgctgagc cttccgacca gctaaccgca
    12781 agttctcatg actagccttg atagcttcaa cagatgacgg attgtcagaa acaaaaccaa
    12841 gatcatccaa ggtcaaaccc atctccccag caaaaccagc ggctgcagtc cgtaactgct
    12901 cagtaaaagg agacatgctg gatgtggtga attgccccac attcggcttg tccccttcat
    12961 catctttggt aaaagtcagc aagctagata cagtcgcttt ccaagtatca atcgcctcag
    13021 catcttgact caatcccaac acatacttct gagggaatga atagaactca gcagtcacat
    13081 ctgagcgctc aagcgttcgt ttagcatatc tctgatagta catcccagct ctagtaattc
    13141 gtgaccgacc aaacggacgg acagcatcag gtctatgaat gactggcacc agcaaaggaa
    13201 cacccgttgg atttccgatt gcaaacggct ttccatcttt aggatagaac caagtcacat
    13261 cactagtaaa ataagcctca agcacggcat aaccattgtc atcccttttc aaaacggcat
    13321 agccctctgt cagcaagcca gtgataggat ccagaacacc agttgcattg cttgcctcaa
    13381 taacctgcaa ccgaggagcg tcatcatcgt ccccttgcga gatgtagaca aaacaacacg
    13441 acccaatcaa tgctgaaagg atcgcgctat caaagaatac atctggattg ttctgagcaa
    13501 agatttcatt cgctccaaat tcgtcattgg caaactcacg aaataccaaa cgatctgcta
    13561 gactgtcaac acccttagca gcccaaccta agaccgcccg atattgttgc ctgatttgag
    13621 gtggtatcgt aataccaaca tctatgtcat tgtattgcat agcatactgt ttgtatctag
    13681 tatctacacc cattttgtaa ttggctagct tcttcctgag atagcccata cctttcaatg
    13741 tcattttata caactacctt tcatttcccg cgagaaaaaa tgtacagtga cggtgtgaag
    13801 ccctgaagca ccgaggggga gggggtcatc cccccacctt ggcaggaaca ctcgtccttt
    13861 tttttaatca cgtttatttt tttaaccctt atacttaaac caatctgtac tttgtggtaa
    13921 gttcctatta ccaatgacct ttgttccgtt tgtcttctca tcagcatata gcttgtcaga
    13981 cttctgtcta ttgcattgcc aatgcgccaa ctgcaagtta gcaatgtcag atggatgtcc
    14041 gttcttattt actggaacga tgtggtcaat gactggactt aatggatgag gatatctcag
    14101 gtctttgtct acaggctggc cacatatccc acaagtgttt cttgtcttta agataatctt
    14161 cttattcttc tcaaaggcta ctctatgggg tccactacgg tccgctcgga gggggttggt
    14221 attcatctag ggagggagtc ctttcttttt aatggagggg gttggtattc tcaaatgtac
    14281 ccctcgatat ctttcaaagt aggggtgttt ttagtgcacc caccccctct tgtatttaac
    14341 atatcttata ttctgttaaa taaaattaaa caacttcaaa gtcaagagta tcaaggcttc
    14401 taccatattt ttctaaaaac taatttacat tttctcatta tgtaaaatag ataggctatt
    14461 tagtaatcaa atgatagtat actctggtca agttcgtctt gactataacc gatatatcct
    14521 agtgtgatgt ctggtgtgga atgattgaat atcttttgta ggatagctac attactattc
    14581 tttttgtaat gatgatatcc aaatgtcttc ctcatcgaat gagttcctat atgattcaaa
    14641 cctacatatt tagctgcatc ttgaagtatt tgatagactg ctactcttcc aatgtgtgtg
    14701 atacgaacac catctgttct ctttttcttt ttacttggaa agagatagtc gtactctgct
    14761 aactgattat ctttaatgta tcgattgatt tcttttctga gaggggggct gattggaaaa
    14821 taccttatct taccagtctt cttctctttt agttcaatcc tatcagcaat tacttgcttg
    14881 acttgaagag gtactatgtc gctcactctt aaaccagagt agattccaaa catgaataaa
    14941 acatagtttc tatcactttt gtttttcaag taatctttga ttcgttctat atcgtctaaa
    15001 tcacgaattg gttctacttt cttcatgtac ctctcctttc ctacataaaa agccactggt
    15061 cgtggcattg aatatgacag tagctggaat tgaaccagct ggtctagcag taaaacgcac
    15121 gtttggtaaa agtttcacgg agacccaaac aacctgctaa cctgtcctta ctgtctaaga
    15181 ggctgaagcc tctatatttt taggagtcct catgactgta cgttgcccaa tcattggata
    15241 atactatttt agcacctttt tccgctccaa ttctcccaag attttcccag atttttccca
    15301 agattttccc agaaatcact tataaactaa aaggttgctc gcttgataag actccgcgaa
    15361 ctctagtaga gctttgttca atatccgata atactcacta gatgagtagc ctagttctga
    15421 ataaatgctg tagtcttccc tccttttctt cctgcaatat cgttcgatta ggatacgtgt
    15481 gtattccata tcagaaagat tgttgattgc tttagcgatt agttctaaat cctgctgagc
    15541 tgacactcta cgcacgacca tgctttctac ttgcttactt gtctgaccac ttgatgacct
    15601 tggttcaagt gagtaggata ttgttatttt gggggcgtat tcttctccag ctatccgtct
    15661 cagacgactg tattttttta gtactttgat agcttccttt ctggttttct tttcgtcgat
    15721 gatatccaat aactctattt gcacacgaac tcctcctcat gatataatag ttatgcgaaa
    15781 ccataccatg aaggtcagcc gtgtgctggc tttttttcat gccctactcc cgagaataat
    15841 atcagcagga aaagcaaagt aagtacagat atcttctaca ttataaatat ccggtacact
    15901 ttcctccttc tcccattgag caattaggta gcgactatag cctagtttag ctgctaactt
    15961 ttcttgtgac aacttccttt ccttacgttt ttgctttagc aagaaagcaa acctacttgc
    16021 ttgtcttgga tttaatttat cattcatcat ccacctcgat ctttacgaca gctctaccat
    16081 ttggatttct tctttgcgtg gatgcaaaag tgtaatactt taacatcctt tcagcaatac
    16141 ctgtttcttt actgatttcc gcaagagttc ccaatgttat gaacacgtcg ccttgatata
    16201 acgcgtattc actcatgttc catctcctca atcagccaat caaggttctt gcgcgctttc
    16261 ttcaggtctt caaggccgtt tttcttctga tgccgtagta tgtacttcaa gctgttaccc
    16321 atatagaacc ctttcagctg ttctggtgtc aggaagtttc ttagagtatc gatagattcc
    16381 attccaaatc taccttggta gtggcttggt ttgtttatgt tgtcaattat ttctgggttc
    16441 attccttatc ctccaaaagt tctgggtttt cgtagatgtt gccgatgatt tcaaattcaa
    16501 aatcttcact tttaataaaa tccttgaaat taagctgctc atcttccatg agaataaaca
    16561 aagaggagtc aggatattta gtttttccaa actcaaaaca aatttcactt ctcaccactt
    16621 caacgtaatt aacgccttcg actgagccgt ctacatagcc ctcgtggcaa tattcattcc
    16681 actcatcatt aaatttcagt atatccccct caaagatttc cttaccattt ctgtcaaaca
    16741 atcctgttga ttgcatgagt tcgatttcat caggataagc tgtaatgtaa tcattcatta
    16801 aagtatcgtt taactcaaat tcttcaatct cgctatcctg aaaaaacata catttgactg
    16861 acatcagtct acctaattca tgatgccaca ctctaaattt cggtttagtg tacggatatc
    16921 tgtttggtcg tttcaattta ccacctcaca tataaatatt tcgtatcaat atcttgttct
    16981 agaatacagt cccttaacga tctcaaaaca tataacgcag cattgaccgt tccccattta
    17041 tttacaggtt catattgtac atatttttca ggttgccttt ccaattcagt tatgccacgt
    17101 ttaatatttt caaaaatatc agcaacattg taaattgtgc cttggtcgaa atcccagttc
    17161 atagcaactc taaacattct tccaagattg tatgtcggag aactattttt aggttcagcg
    17221 atacaaatat aatctccgct ctctattttc gctaagattt ccaaatcata actcatcact
    17281 ccacctcctc aaaataacta tgaaatttac ttaaattgac aatagcgacc tcttcaacag
    17341 aatgctttcc gatgtcaaag tctggatcat tcttcccaaa ctctttttct atagcttttt
    17401 cagccagaaa aggtaaagcg aatatacttg ctccgttttt taaggcaagc gcttgaccgt
    17461 gtttatttac tattcgataa cccacatcaa acggtctgat ttccattggg atttttatgc
    17521 atttactttg attcttcatt ccttcttcaa gtgtttgtgt catcactcca cctcctcaat
    17581 ctcaattcct tcacaatcaa acacccatcc gaggccagct tcttctagtt ctttacgggt
    17641 gtgggaatat ataacatcgt ctaaactaaa gctttttgta aagaaatacc ttttcaaaag
    17701 ttctccataa accaacatat tttctttaat attcccttta atcttaacaa aataccgctt
    17761 ctctttttcg acctcgtagc cgttaagcca agcaagacag aatttttcga tgttattttc
    17821 gtaaaaccaa tcaggaactt tcttatcata atgatcttca attactctca ttgcaccgta
    17881 aacatgaaaa ttgtttttct ttttaaattc tatatattcc gccacacact gcggaacttt
    17941 gactttttct ggttcgtcta gttgtttcaa ctctttcaaa aaaagttcaa ccattgaagt
    18001 ataaggtaca ggttcataaa aagggctgcg ttcattccac aattcttcat acttcttaat
    18061 caatgcttta atattcatct tagtttcctc cataaatcaa ataaactgca ataactacct
    18121 gagccatgct tggcgcatag ccaatccaat catcaaactc cttagatttt ggcaaccaac
    18181 ccttagtagc tcccaaatca tagtctgtag gcttttcatc agcaaagatg cattccatcg
    18241 ctcccataaa cgtcatacca tcttctgcca tttcccaaaa atagtccgcc cggtctttca
    18301 ccacttgtgg taaatcttgc ttgggaggtt gcggcttccc gtcttctacc gtccagttgt
    18361 atactgcatt aactttttgc tttaactctt ccatcatctt ccaactcctc caatttccgt
    18421 ctcaattctt tattcttttt cctcaacaaa tcgcgctcca gcgctctaat ccgtctcttg
    18481 cgtgcatcgc acggcttcga atactcgatt atcttctctt cgttttgctc gatcgtgcgt
    18541 ttcagtccgt caatctcagc ctgtttatcg tacttcatct tctaaaaatc tttcaatagc
    18601 ttctctgtag gagacttcca ccagaccgtc taagtcgttc agggcttcaa tatagtctgg
    18661 acgaccttgc ccgtactgct ctttcaaaaa ttcaacaaag agatgaattt cctgataggt
    18721 tactccaacc atgtttctta cctcccacta aaaatgtaac gttattgatc atctttcttt
    18781 tcctttcttg ctgcacgttc cccgactaaa tagccgagaa ataaccacag aatagccatt
    18841 ccaaattctt taataagttc aatcattttt ttctcctcct gaaaaagtcg ctaaatagta
    18901 acaatcctta gaaccgtagt caaatcgtgt cgtccgctga ccaatgtgct tctgaaacct
    18961 tggatgagtg atagctgaga aagcccactg atggtcttcc atctgctcaa tgagatcatc
    19021 gacattgtca aacctcccaa ggaaaaactt acagtgcccg ttgtagacga aataaagctc
    19081 taacatcact ccacctcgac agggtagaag ttcccaaagg aacctctcaa tgccttgcta
    19141 acctgtaagg ctgccgcccg agaaataaac cgcatggctt tcttctcctc agaacacgaa
    19201 atatccaagc cagtcacact gataattgca gaccttagaa acggcttatc ctctcttgtc
    19261 ccatgcttta aaataaacat cagccacccc tattctaaaa atattgcttt cgcttgtttg
    19321 tcaaatcatt gaaaaccatc aaatggtctt tatctacacc cttcattagt ctggacatga
    19381 aaggtctgcc atatcttttt tgaatatcgg cagaaatcaa atttgtggta atgattgtgt
    19441 ttgaacgctt attcaagata ttgtagagaa tagtaaatga ccattcgcta tctttttcca
    19501 ttcctaaatc atccaaaacc aagaacttag cactagcaat tttattgacc agaaactctt
    19561 cctgactaaa atcagcttta atcttcatca gcaagtcagt tacgttaata aaaatagcaa
    19621 tctctttcgt gtactcagat agagccttaa ccatagcaaa agccaaatgg ctcttacctg
    19681 ttccagcttc tccttgtaac acgatgttgt tcctagcacc ctcataccac tcacgacaaa
    19741 tcctctttgc aaaagctagc ttttccgctt ctttttcggt tggtgtttca aaattgtcca
    19801 aagtagcatt tttcaaaact tcatcataaa gagagaactt ttcaagatag tatttcctct
    19861 ctcgctcatt ctcagcatta gccagttcat tcactcttgc ttgattctcc tcatggatcc
    19921 gctcagattc gcacattcgg catacaacac tctcagtccg caatatcttt ataaaaggaa
    19981 tgttatgctt ttcgcaaatt tcagtctgct gttcagtatt tctatggtaa gataaggcca
    20041 tctcctctag tgcatcagtt accatgatac tttaccccca caagctttcc agctagccat
    20101 atctgacaag caagtaatga cagtagaaag aggttgtttt ataagcaaag acttcttttc
    20161 ctcgcttata ggataaaatt cctcttcaaa ttgctttata agttctaaaa cccccattcg
    20221 ttttttacct cctgctctga ttttttatca tttcgttgtc ttcccgaacg gttatagttc
    20281 ttactatctt tatatttttg gtcattatca tctacctgct caatagttgt gaaacctttc
    20341 tttttccaat tttcaagaac tcctcttaaa tacttgaaac taggtcgata aacctccgaa
    20401 gttatctcaa ttgcacggtt taacatatca aaactcattc catcaagtcc tacatattca
    20461 agcaactgtt gatgttgttt attgtttatc cgaataccac tatgtttcaa attttcagat
    20521 aagctggaac taaccatcgt cttattactt tctttctcta tatctttatc tatatcttta
    20581 tctatctctt tatctgtatc tatatctata tctccgttac actttgtttc atcggtgtta
    20641 cattgtaacg ctaatttatt ctctcgaaac ttgcgaaccc ttctggcgct tgcagtttca
    20701 ctacctacca tctcaggaac ttgctctaaa aaataatcac ggtcagagtt tctagtcagc
    20761 aatcctttgc tttctaaaaa aatcaatgta attttaatat cttcaacatt ttcatcaatg
    20821 acaagagcga tttcctcagc tagattgtca gccaatccat cgtagtaaat atgccctcca
    20881 tcttccaagc taattaacat cattttgagg taaatgatgg tatgtgtatc cccaccagca
    20941 atcttacgaa gtagtttcat ctcctttgac ttaaagaagt cctgagcaag ctggatccag
    21001 aaataccgct tgttttttaa cgccataatc tcacacccca cttcctacgg ttagcgcggt
    21061 acttcatccg catatcctca tggatgcgcc tgccttccag ctccattttt tcaagcttta
    21121 gcagtttatt ttcagagacc acatcacgat agtccttggc tagttttcca tagtcggtta
    21181 ggtattcttt gataagtgaa attttcctgt tctcgtcctc taaatatagt tcaaaatcag
    21241 acttttcttc atcagacgat aacatttctt tattcactct ctcatgccac aatagccatt
    21301 caatcaattc ttccatttcc tgacctcctc atgataaaaa tctgattgca gactgtttag
    21361 gctctggcaa agctaatggt tcaggacgca aacctacagg cggttcgttg tcataggtga
    21421 atcctttgaa ctctctgcga atattcttgc gaatttcttg ccatttgtcc tctctaccac
    21481 gttcgtatgc atagtttcgc ccttggataa tcatagacgc aaattcttgc tcttctcgtc
    21541 tttctttttc cttacgctcc tcttgcaatt tgatatgacg gcaaagccct gcaaatccaa
    21601 tcagcaaagc tccaacaccc atcagttggt ctaaaatcgg tggttcaaac atttttatct
    21661 ccttatgctc tttttgtgtt ataatatagt caaataattt tgctaagacc ttgtccagaa
    21721 gccttttagt aaagttatta tatttgatta gagagccatt ctttgatggc tctttttgac
    21781 catttcttac caggtaattc ctttggaaat ccctttaagt aacgataatt atctgaaaat
    21841 gtggcatact taattcctag aaattcgcag gtagtgttca catccatcaa ctctggatag
    21901 tgatcactat ctttttctat ttcgactagc cttgtgattg tgtccttgat aatagattta
    21961 atccattcag atagtgaaag tagaacattg tccatcttct tcccctccta cccttcgtca
    22021 aatgagttca atttcatgat tttcatcttg gtattagtgc ttggctccca cgtcatccag
    22081 taagcaagag cggcatccgc atgcttcttg ggtagtaagt catagcgact gatattaaaa
    22141 tgatccttga aatcaatctc agcttgtcta aataccgact gagcgaaaat cttatcagca
    22201 taagcaggac tatcaatacc acctaagcaa gccacgacac gagctttgcg cttcttcagg
    22261 agcgactgag catagcttgg atgaatcggt tgctcgctct tgaggtagtc gatatcttcc
    22321 agcatgctag tctgttgctc ccgcaatttc ttctggccag taaaaagagc gataaaggca
    22381 tcctcgtcca agtcctcacg gataaaacca ccctgcttgc gaatagctgg caaaacctct
    22441 gatgtcaccc aacgcttgaa ctccttagct tgaggcaact tgcttgataa gataagagag
    22501 tagagacctg attcgttgat gactgtctga tttctcatct gacctgccgt cgcgatttgc
    22561 gacgttagct tatcatcttc atcaacgtgt tttgccagag catctctaga atttacatat
    22621 ccaagaatct ctgctatatc tttcccgaca aaccacggtt cgtcatcaaa catcactgta
    22681 cggacttcct gcccatgaaa gttaaaaatt tcgttcatag tattcctttc ttcaatttgt
    22741 cgcacttatg cgactgtttc gctaaaaaaa atagataaag cttcgtcttt tgaaagaccg
    22801 agggaagaaa caatcaagtt tacttcttgt atagagaaac taccattttg tttcattttt
    22861 ctatagaacg tactcttatc aatcccaata tcttttgcta aagcctcttg agtagtattg
    22921 cgttcgataa tttttccttt taatttcgat acatttacca tatctgctcc ttttcatttg
    22981 tcgcatttac gcgacttatt gttttaagta taactgataa aaaatgaaat gtcaataaga
    23041 aaatcgcatt tttgaaactt tttatattgc atttttgcga ctaatgttgt aaaattattg
    23101 tgtaatatat aataaggggt aaaaaaatga atgtcggaga aagaataaaa caacgtcgaa
    23161 aagcgttgaa aatgtctgcg gacgagcttg cagaaagtgt aggtgtctct cgttctacta
    23221 tttttaggta cgaaaaagga gatattgaaa aagttggacc tgaagtatta aaaaaaatcg
    23281 ctgacaaatt aaatgtatca cctggagact tgatgggatg ggaagacaat caacaagaat
    23341 tgaaaatccc aacctccccg ttggttcaca aaattactga aaaggttgtc aaactatcaa
    23401 ctccgagaaa acaaaaggtt cttaactacg ctaacgaaca attgaaagag caaaataata
    23461 aagtaatcac aattgaggaa aagctttttg aatatcgtgt ttttgaaaaa ctttcagctg
    23521 gtactggatt ctcatacttc aacgatggga actatgacac tgttttttac gacaaagacc
    23581 tagaccacga ttttgcttct tgggttttcg gagattccat ggagcctaag tacatgaatg
    23641 gagaggtcgt tctcatcaaa gaaacaggtt ttgactacga tggtgccatt tatgcagttg
    23701 actgggatgg tcaaacttat attaaaaaag tctataaaga aaaagacggt cttagactcg
    23761 tctctatcaa taacaaatat aaagataaat tcgcaccatt tgaagaagat ccaaggatta
    23821 ttgggaaaat agtcggaaac ttcatgccaa ttgaaaatta aaaggagaaa gttatgaaaa
    23881 taggaatgag aaaaccaagt ctgaccagaa gtttaaaagc tagaactact agtaaatgga
    23941 agagacaggc aaaaaaagcc cttatccctg gctatggtaa aaaagggatg ggatgggtta
    24001 aaaatccaaa gaaagctatg tataataagg tttatcataa gacaaccttt ggactttcgg
    24061 atttgttcaa atcgtccaaa aagagcaaga agaaagtaga caccaataaa caacaatcta
    24121 ttttgtcatc tagcggtaaa aagcaacaca ctccaaaaga atataaagaa gctggacttg
    24181 tctttatggt tttaggtgct atattcctat ttttatttcc acctctcggc ttcttcttgt
    24241 ttattactgg ttttataact tacattattg gtcgtttaac tgcaaagcga gagaaaaagc
    24301 agaaagttga aaattacagt ccacagattg atacaattgt tttccgagat aattttttat
    24361 taatgggaac aaattatcat caagaagaag ctgagattgc agctgatttt ctttccaagg
    24421 gtgtccatta ttttgggaaa gataataaga gtttgaaatc ttatatgctt gaaacatata
    24481 aacctgttta caaatacaat aaattgaaaa cagtagacgt tcaactatta ccagaacctt
    24541 caaatccgca tgataaaaat gctgtcaaag ttttagtgaa taatatcttt gttggatact
    24601 taccagctgt gatcgcatca caaatttcat catacatagc taatccaaat taccgatacg
    24661 atgcaatcct tactggtaga ggtggaccat ataaaaccct aaatattgaa accgagagag
    24721 ttgttactcg taaaaaagaa ttaacgtatt at
//
LOCUS       DBEJAQ010000030        24482 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.030, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000030 DBEJAQ010000000
VERSION     DBEJAQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.030"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="ACL297_001325"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="ACL297_001325"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGR0071585.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1319..2023
                     /gene="yycF"
                     /locus_tag="ACL297_001326"
     CDS             1319..2023
                     /gene="yycF"
                     /locus_tag="ACL297_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HGR0071586.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2016..3365
                     /gene="vicK"
                     /locus_tag="ACL297_001327"
     CDS             2016..3365
                     /gene="vicK"
                     /locus_tag="ACL297_001327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HGR0071587.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3367..4176
                     /locus_tag="ACL297_001328"
     CDS             3367..4176
                     /locus_tag="ACL297_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGR0071588.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4267..4509
                     /gene="relB"
                     /locus_tag="ACL297_001329"
     CDS             4267..4509
                     /gene="relB"
                     /locus_tag="ACL297_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HGR0071589.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4511..4765
                     /locus_tag="ACL297_001330"
     CDS             4511..4765
                     /locus_tag="ACL297_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGR0071590.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4812..5555
                     /locus_tag="ACL297_001331"
                     /pseudo
     CDS             4812..5555
                     /locus_tag="ACL297_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
     gene            complement(5856..6842)
                     /locus_tag="ACL297_001332"
     CDS             complement(5856..6842)
                     /locus_tag="ACL297_001332"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGR0071591.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7036..9504
                     /gene="gyrA"
                     /locus_tag="ACL297_001333"
     CDS             7036..9504
                     /gene="gyrA"
                     /locus_tag="ACL297_001333"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGR0071592.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9504..10247
                     /gene="srtA"
                     /locus_tag="ACL297_001334"
     CDS             9504..10247
                     /gene="srtA"
                     /locus_tag="ACL297_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HGR0071593.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            10491..11288
                     /locus_tag="ACL297_001335"
     CDS             10491..11288
                     /locus_tag="ACL297_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGR0071594.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            11436..12695
                     /locus_tag="ACL297_001336"
                     /pseudo
     CDS             11436..12695
                     /locus_tag="ACL297_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     gene            12791..14065
                     /locus_tag="ACL297_001337"
     CDS             12791..14065
                     /locus_tag="ACL297_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HGR0071595.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELCLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            14075..14953
                     /gene="truB"
                     /locus_tag="ACL297_001338"
     CDS             14075..14953
                     /gene="truB"
                     /locus_tag="ACL297_001338"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGR0071596.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            15194..15496
                     /locus_tag="ACL297_001339"
     CDS             15194..15496
                     /locus_tag="ACL297_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGR0071597.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            15604..16242
                     /gene="udk"
                     /locus_tag="ACL297_001340"
     CDS             15604..16242
                     /gene="udk"
                     /locus_tag="ACL297_001340"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGR0071598.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            16370..17710
                     /gene="xseA"
                     /locus_tag="ACL297_001341"
     CDS             16370..17710
                     /gene="xseA"
                     /locus_tag="ACL297_001341"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGR0071599.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            17688..17900
                     /locus_tag="ACL297_001342"
     CDS             17688..17900
                     /locus_tag="ACL297_001342"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGR0071600.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            17897..18772
                     /locus_tag="ACL297_001343"
     CDS             17897..18772
                     /locus_tag="ACL297_001343"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGR0071601.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            18765..19580
                     /locus_tag="ACL297_001344"
     CDS             18765..19580
                     /locus_tag="ACL297_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HGR0071602.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            19573..20004
                     /locus_tag="ACL297_001345"
     CDS             19573..20004
                     /locus_tag="ACL297_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGR0071603.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            20011..21678
                     /gene="recN"
                     /locus_tag="ACL297_001346"
     CDS             20011..21678
                     /gene="recN"
                     /locus_tag="ACL297_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGR0071604.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            21680..22408
                     /locus_tag="ACL297_001347"
     CDS             21680..22408
                     /locus_tag="ACL297_001347"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGR0071605.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            22487..24310
                     /gene="lepA"
                     /locus_tag="ACL297_001348"
     CDS             22487..24310
                     /gene="lepA"
                     /locus_tag="ACL297_001348"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGR0071606.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         7401 a         4324 c         5667 g         7090 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat tcctggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccgg atcgtccggg
      661 tgattttaat caagccttga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctttggcccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttg gtataatagt agagaaaaag gtgaacatat
     1321 gaaaaaaata ctaattgtag atgatgagaa accaatctcg gatattatca agtttaatat
     1381 gaccaaggaa ggttacgaag ttgtaactgc ttttaatggt cgtgaagcgc tagagcaatt
     1441 tgaagcagag caaccagata ttattattct ggatttgatg cttccagaaa ttgatggttt
     1501 agaagttgct aagaccattc gtaagacaag cagtgtgccc attcttatgc tttcagccaa
     1561 agatagtgaa tttgataagg ttatcggttt ggaacttggg gcagatgact atgtaacgaa
     1621 acccttctcc aatcgtgagt tgcaggcgcg tgttaaagct cttctgcgtc gttctcaacc
     1681 tatgccagta gatggtcagg aagcagatag taaacctcaa cctatccaaa ttggggattt
     1741 agaaattgtt ccagacgcct acgtggctaa aaaatatggc gaagaactag acttaaccca
     1801 tcgtgaattt gagcttttgt atcatttagc atcgcataca ggtcaagtca tcacgcgcga
     1861 acacttgctt gagactgtct ggggttatga ctattttggt gatgtccgta cagttgatgt
     1921 gactgtacga cgtctgcgtg agaagattga agatacgccc agccgaccag agtatatctt
     1981 gacgcgccgt ggtgtagggt attacatgag aaataatgct tgatttactg aaacaaacca
     2041 tttttaccag agattttatc tttatcctga ttttgttagg tttcatcctt gttgtgaccc
     2101 tcttattact ggaaaataga cgtgataata ttcagttgaa gcaaatcaat caaaaggtta
     2161 aagatttgat tgcaggagat tattccaagg ttcttgatat gcaaggtggg tctgaaatca
     2221 ccaatattac caataatttg aatgacttgt cggaggttat tcgtctcact caggaaaatc
     2281 tagaacaaga gagtaagagg ctaaatagta ttctgtttta tatgacagat ggggttcttg
     2341 cgaccaaccg tcggggtcag attatcatga ttaacgatac agccaagaag caactggggt
     2401 tggttaagga agatgttctg aatagaagca ttttggaatt gctcaagata gaagaaaact
     2461 atgaattgcg tgatttgatt acccaaagtc cagaattgtt gctagattcc caagatatca
     2521 atggcgaata tttgaacctt cgagttcgct ttgccttgat acgtcgagag tctggcttta
     2581 tttcaggttt ggtggctgtt ttgcatgata cgacggagca ggagaaggaa gaacgcgaac
     2641 gaagactctt tgtttccaat gttagccatg agttacggac tcctctgact agcgtaaaat
     2701 cctatcttga agccttggat gagggggctt tgtgtgaaac tgtagcacca gactttatca
     2761 aggtttctct tgatgagacc aaccgtatga tgcgcatggt gacggatctc ctccatcttt
     2821 cacgtattga taatgctacc agtcacctag atgtggaact gattaacttc actgctttta
     2881 ttacctttat cctcaatcgt tttgacaaga tgaaaggaca ggaaaaggag aaaaaatatg
     2941 agttggtgag agattatccc atcaattcta tctggatgga aattgataca gataagatga
     3001 cgcaggttgt cgacaatatt ttaaataatg ctattaagta ttcgccagat gggggtaaaa
     3061 tcactgtcag aatgaagaca actgaagacc agatgatttt atccatttct gaccacggtt
     3121 tggggattcc taagcaggat ttaccacgta tctttgaccg tttctatcgt gtggatcgtg
     3181 ctagaagtcg tgcacaaggt ggtacaggtc taggactgtc tatcgctaaa gaaattatca
     3241 aacaacataa gggctttatt tgggccaaga gtgaatacgg caagggttca acctttacca
     3301 ttgtgctccc ttatgataag gatgcagtga aagaagaagt atgggaggat gaagtagaag
     3361 actagaatga gtgaaatagg ctttaaatac agtattttag cgtcgggttc cagtggaaat
     3421 tctttttatc tggaaacctc aaaaaagaag cttttagtag atgcaggctt gtctggcaag
     3481 aaaattacca gtctgctagc tgaaattaac cgtaagccag aagacctgga tgccatcttg
     3541 attacccatg agcattcaga tcatatccat ggagtaggcg ttttggctcg caagtatggt
     3601 atggatcttt atgccaatga aaagacctgg caagctatgg aaaatagtaa atatcttggc
     3661 aaggtggatt cttcgcaaaa gcatattttt gaaatgggta aaaccaaaac ctttggagat
     3721 atcgacatcg agagttttgg tgtaagccat gatgcagtcg caccgcagtt ctatcgcttt
     3781 atgaaggatg ataagagttt tgtccttttg acagatacag gttatgtcag tgaccgtatg
     3841 gcgggcattg tcgaaaatgc ggatggctat cttatcgagg ccaaccatga tgtagagatt
     3901 ttgcgatcag gttcttacgc ttggcgactc aaacaacgaa tcctatctga ccttggtcac
     3961 ctttctaacg aggacggtgc tgaagctatg attcggacgc taggaaatcg tactaagaag
     4021 atttaccttg ggcatttatc taaggaaaat aatatcaagg aactggctca tatgaccatg
     4081 gtcaatcagc tggctcaagc tgatctggga gtcggagtag actttaaggt ttatgatacc
     4141 tcaccagata ccgcaacacc attgacagag atatagaaag aacgctgaga aggtgttctt
     4201 tttatattga ctgaacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca
     4261 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
     4321 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
     4381 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
     4441 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag
     4501 gatgaataat ttgtataaat tagttccaac aagacgtttt atcaagcaat tgaaaaaatt
     4561 ggaccgttat acgcagaagc taattacaaa ctatttacaa accaatgttt tggaaggtcc
     4621 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
     4681 taattatcga gttatcgtac aaattgtaga tgatgaatta gtcgttgcta ctctagaagt
     4741 tggtcatcgg agagatattt attgaattaa agctgagtaa taacaatata gaaagtaagg
     4801 agagtaaaaa tttgaagaaa aaatggttat ttgcagatta ttatgataca acaattattt
     4861 tacttgcttt aatctctgtt attctggtat tacttggatt tgctgagatg actgatttgg
     4921 acaatccgcc ttatagtatt attgatttag taatctgggg tgtttttgta attgattata
     4981 gttggcgctt ttttattact aaaagaaaat ggcgctttat tcttgaaaat atttttgatt
     5041 tattgaccat tctcccttta aatgctattt ttacagtgtt tcgattagga cgtatcttcc
     5101 gcttagcaag gctgacaaaa ttactgaaac tgactcgttt acttagaata attggactta
     5161 caggtaaact agaaagaaaa attagtagat tcttacgaac aaatggattg atttatatct
     5221 tgtgtgttaa tatctttatt gttctagtag ggagttcgat tttatccgtt gttgaagaaa
     5281 aatccttctc agatagtctt tggtgggctc ttgtgacagt aactactgtt ggttatggtg
     5341 atattgttcc agtttccctt tttgggaaat ggctagcagt tttactgatg cttgtcggta
     5401 ttggaacaat agggatgcta acaagtgctt tgacgaactt ttttgtaaag gataatccag
     5461 atgaacagat aaaacttgat aaactcaaag acgaattatc gagctaaaga atattaatag
     5521 agaaacaatc tgaaaaaatc gaagaactac attgaatgat aaaagactta gttgaaaaaa
     5581 tatcataaag gtgagacaaa ataaaacttg attcgaggag aaatcaagta agttttgaca
     5641 caatagaacg catattatca ggtataaaaa cttgatacta tgcgttttat agagaaataa
     5701 aataaaatct gaacaaatca attaggaatt taaagcattt tataacaacg ttttagagta
     5761 atggggtgct atttcaactt caacctacta taatacagaa aaaaatacag aaaaaaacaa
     5821 ctccctgatg attcaaggag ttgtctatag ttaaattagt ttttagaagc ttcttggaat
     5881 tctggatttt tccatgcttc gtcaataata gcttgcaatt ctttagcaga tgcttgcatt
     5941 ttttgagttt ctgcatcgtt caatggaatg tttactggac gaacgatacc atgtgcacca
     6001 acaacagctg gttgaccgat aaagacattc tcaactccgt attgaccttc ttggaatact
     6061 gaaagtggaa gtactgcgtt ttcatcgtca aggattgctt tagtgatacg agcaagggct
     6121 actgcgatac cgtagtatgt tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga
     6181 acaccttcga acaattcaat caattcagct tcttgaacat tttgagtgtc tttaaggaat
     6241 tcttcaaggt ttacaccagc gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg
     6301 tgttcaccca tgatgtaggc gtgcactgaa cgagcatcca catccaattt ttcagcaagt
     6361 gcttgacgga aacgagctga gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg
     6421 aaaccagaga atttccaagt tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg
     6481 aagatacctt tgaaaccaga ttcaacaact tgagttacga ttgatttgtt gatagcaagg
     6541 tttttaccta caaggtcaag acgagtttca cctggttttt gaggtgcacc tgcagtgatc
     6601 acaacaaggt cagcgtctgc acagtcagag tattgagctg catagatttt tttaggtgaa
     6661 gtgaaggcaa gggcgtgact aaggtcaagc gcatcaccaa cagctttttc atgcaattgt
     6721 ggaatttcga taattccaag ctcttgtgca attccttggt taacaagtgc aaaagcgtaa
     6781 gatgaaccta cagcaccatc accgacaagg ataacttttt tgtgttgttt agttgaagtc
     6841 attgttttaa acatctcctt aattttatta ggggattttc cctagacaac ttcattctat
     6901 cacttttaaa aaactttgtc acgaatatgc cttatagttc tcgatgtaaa cgttttagtg
     6961 gtttagaggc tgaaatagat gggaatttat ggtataatgt tgttacttac taattgtgaa
     7021 atgaggcatt tattaatgca ggataaaaat ttagtgaatg tcaatctgac aaaggagatg
     7081 aaggcaagtt ttatcgacta cgccatgagt gttatcgtag cgcgagctct tcctgatgtt
     7141 cgagatggct taaaacctgt tcaccgtcgc attctctacg gaatgaatga attgggtgtg
     7201 accccagaca aaccccataa aaaatctgct cgtattacag gggatgtcat gggtaaatat
     7261 cacccacacg gggattcctc tatttatgaa gccatggtcc gtatggctca atggtggagc
     7321 taccgttaca tgcttgtaga tggtcatggg aattttggtt ccatggatgg agatagtgct
     7381 gccgctcaac gttataccga ggcacgtatg agcaagattg ctctggaaat gcttcgtgat
     7441 atcaacaaaa atacagttga tttcgttgat aactatgatg ccaatgaacg ggaacccttg
     7501 gtcttgccag cgcgttttcc aaaccttttg gttaatggag caactggtat cgcggttggg
     7561 atggcaacca atattccacc tcataatctg ggtgaaacca ttgatgcagt gaagttggtc
     7621 atggataatc ctgaagtgac taccaaggac ttgatggaag tcttgcctgg accagatttt
     7681 ccaactggtg ctcttgtcat ggggaaatca ggtatccata aggcttatga aacaggtaaa
     7741 ggttcgattg tcctacgttc tcgtacagag attgaaacga ctaagactgg tcgtgagcgt
     7801 atcgttgtaa cagaatttcc ttacatggtc aataaaacca aggtgcatga gcatattgtt
     7861 cgcttggttc aggaaaaacg cattgagggt atcacagcag tacgtgatga gtcaaaccgt
     7921 gaaggtgttc gatttgttat tgaagtcaag cgcgacgcct cagccaatgt tattctcaat
     7981 aacctcttca aaatgaccca aatgcaaacc aattttggat tcaatatgct cgctatccaa
     8041 aatggtatac cgaaaatttt gtctcttcgt cagattttgg atgcttatat tgagcaccaa
     8101 aaagaagtgg ttgttcgtcg tacacgtttt gataaggaaa aagcggaagc gcgcgctcat
     8161 atcttagaag gtctcttgat tgcgctagac catatcgacg aagtgattcg tatcatccgt
     8221 gctagtgaaa cggatgcgga agctcaagct gagttgatga gcaagtttaa gctttctgaa
     8281 cgtcaaagtc aagctatcct tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat
     8341 aagattcaat ctgagtatga tgacctcttg gctctgattg cggatttagc agatattctt
     8401 gctaagcctg aacgtgtttc tcaaattatc aaagacgaat tggatgaagt taaacgtaaa
     8461 ttttctgata aacgccgtac agagttgatg gttggacaga tcttgagtct cgaggatgag
     8521 gacttgattg aagaatcgga tgtcttgatt accctttcta acagaggcta cattaagcgt
     8581 ttggatcagg acgagttcac tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga
     8641 gtgaaagatg atgactttgt tcgtgagtta gtgtcaacta gcacccatga tcatctgctc
     8701 ttcttcacaa acaagggacg tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt
     8761 cggactgcca aagggctacc agtagtcaat ctcttgaaat tggatgaaga tgaaagtatt
     8821 cagacggtta tcaatgttga gtctgatcgc agtgatgatg cttatctctt ctttacaacc
     8881 cgtcacggta ttgtgaagag aactagtgtt aaggagtttg ccaatattcg tcaaaatggt
     8941 ctcaaagcgc tgaatttaaa ggatgaagat gagttaatca atgtcttgtt gacagaagga
     9001 gatatggata ttatcattgg taccaagttt ggttatgcag ttcgctttaa tcaatcagcc
     9061 gttcgtggta tgagccgtat cgccactggt gtgaaaggtg ttaaccttcg tgaaggagac
     9121 acagttgttg gtgccagctt gattactgat caagatgagg ttcttattat cacagaaaaa
     9181 ggatatggta agcgtacagt cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga
     9241 atgcagacag ctaaaattac cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa
     9301 ggggatgagg atttgatgat tatcactgat acaggtgtca tgattcgaac caatcttgcc
     9361 aatatttcac aaacaggacg tgcaactatg ggagttaaag taatgcgcct ggatcaagat
     9421 gctcagatag tgactttcac aacggttgcg gtggcagaaa aagaagaagt tgggacagaa
     9481 aacgaaacag aaggtgaagc ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa
     9541 aaaatctgct gacaaatatc ctagcaggat ttctgatatt actgtcactg gctttgattt
     9601 ttaatactca aattcgaaat attttcatag tctggaatac caataagtat caagttagcc
     9661 aggtatcaaa agaaaaatta gaagaaaatc aggatacaga aggcaatttt gactttgatt
     9721 ctgtcaaagc tatctcttcg gaagctgttc taacttctca atgggatgct caaaaattac
     9781 cagttattgg gggaattgca attcctgaat tggaaatgaa tttgccgatt tttaaaggac
     9841 ttgataatgt taatctcttc tacggagctg gtacaatgaa acgcgagcaa gtaatgggag
     9901 aaggaaatta tagtctagct agtcaccata tctttggtgt tgataatgct aataaaatgt
     9961 tattttctcc tttagataat gctaaaaatg gcatgaagat ttatctaacc gataaaaata
    10021 aagtttatac ttatgaaata cgtgaagtca aacgtgtgac accggatcgt gttgatgaag
    10081 ttgatgatag agatggggtc aatgaaatca cattagtaac ctgtgaagac cttgctgcta
    10141 cagaacgtat tattgtcaaa ggtgatttga aagaaacaaa agattattca caaacatctg
    10201 atgaaattct aacagctttc aatcaaccat ataaacaatt ttattaatac aaatcagtga
    10261 aatcatggat ttcactgatt ttttaagatt ttcataaaaa tatagtaaaa tgaaataaga
    10321 ataggacgaa tcgttcagga cagtcaaatc gatttctaac aatattttag aagtagaggt
    10381 gtactattct agtttcaatc tactataaac ttttatgaaa tacagaaaac gcttcctaaa
    10441 acctaaagtt tgtgatataa ttatcaagaa aaaagattta ggagtttatt atggtttctt
    10501 cagaatttat ctcaaagatt gaatttgctt gcaataagaa agaaagtctt tatagtcaaa
    10561 gcaaatttaa gtatgcgatt cgttcgatgt tcgcaggtgc atttttaacc ttcagtactg
    10621 ctgcaggtgc agttggggct gacttgatta ataaaattgc accaggtagt ggacgcttcc
    10681 tctttccatt cgtttttgct tggggcttgg cctacattgt ttttttgaat gccgagttgg
    10741 tcacttcaaa catgatgttc ttgactgctg gtagtttctt aaaaaaaatc tcttggagaa
    10801 aaacagctga gattttacta tactgtacct tgttcaacct tatcggagcc ttgatagcag
    10861 ggtggggctt tgctcattcg gcagcctatg cgaatctgat acacgatagt ttcatctcag
    10921 gtgttgttga gatgaagtta ggccgctcaa atgaattggt cttgcttgag gcaattttgg
    10981 caaatatttt cgtaaatatt gcgattctgt catttatttt ggtcaaagat ggtggtgcca
    11041 aactttggct tgtgttgtca gctatttaca tgtttgtatt cttaacaaac gagcacattg
    11101 cggcgaactt tgcttctttc gcgattgtga aattcagtgt tgctgcggat tcaattgcca
    11161 acttcggtgt tggaaatatg cttcgccact ggggtgtgac tttcatcgga aactttatcg
    11221 gaggaggcct cttgatgggt cttccatatg ccttcctcaa taaaaacgaa gatacttatg
    11281 tagattaaga aaatgagcac gattgagtcg tgcttttttc attttcaaaa taaggtaata
    11341 gctatttctt atatcaaaat atagaaaact gatatttgta aactataact caaggtgcta
    11401 caatatcctt aataaaatga tatggaggtc accttatgac ttgtgatttt aaatctgaaa
    11461 ctctacaact acatgctggt caagttgtgg ctccagctac taagtctcgt gcagtgccga
    11521 tttatcaaac aacatttttt gtttttgatg acacgtagga aggtgccgat ctgtttgcct
    11581 tgaggaaacc agggaacatt tatactcgta tcaccaatcc tacaacagct gcccttgaag
    11641 gtggtgttga agcgctagca acagcatcag gtatgactgc agtgacttat acgattttgg
    11701 cgcttgccca tgctggtgac catgtagtgg ctgcttcgac tatttacggt ggaaccttca
    11761 atcttttgaa agaacccctt cctcgttatg gtatcacaac aacctttgtc gatgttgata
    11821 atttggagga agtagaagca gctatcaaag acaataccaa gcttgtcttg attgaaacct
    11881 tgggtaactc cttgattaat attccagacc tggaaaaact ggcagagatt gctcataaac
    11941 atcaaatccc acttgtgtca gacaatactt ttgcaatacc ttatttgatt aacgtcttct
    12001 ctcatggcgt tgacattgcc attcactctg cgactaagtt tatcggtggg catggtacaa
    12061 ctattggagg aataattgtc gatagtggtc gttttgactg gatggcttca gggaaattcc
    12121 ctcaatttgt tgacgagggt tcaagctgcc acaatttgag ctatactcgt gatgtgggtg
    12181 cagtagcctt tattatagct gttcgagttc aattgcttcg tgatacaggt gcagccttgt
    12241 caccattcaa tgctttcctc ttgctacaaa gacttgaaac ctcttcactt cgtgtggaac
    12301 gccatgtaca aaatgctgag acaattgttg attttcttgt caaccatcct aaggtagaaa
    12361 aggtaaatta tccaaaactt gcagatagtc cttatcatgc cttggctgag aaatacttgc
    12421 caaaagatgt cggttcaatc tttaccttcc atgtcaaagg tggcgaggaa gaagcacgca
    12481 aggtcattga tcatttagaa atcttttctg accttgcaaa cgcggcagat gctaaatcgc
    12541 ttgttgtcca tccagcaaca accactcacg gtcaattgtc agaaaaagac ctagaagcag
    12601 caggtgtcac accaaactaa attcgtttgt caatcggtct tgaaaatgta gaagatttga
    12661 ttgaagactt gcgcttggcc ttggaaaaaa tttaaagtaa aagaagataa acagtgggct
    12721 tcgactcact gtttttgatt ttccctcagg catgatataa tggttaaaga agtctagaaa
    12781 gaggaacgat atgaacgaaa tcaaatgtcc caactgtggg gaagtcttta cagtaaatga
    12841 gagtcagtat gccgaactct tgtcccaagt gagaacggca gagtttgata aggaactaca
    12901 cgataggatg aagcaggaac tggccttggc tgagcaaaag gccatgaatg agcaacagac
    12961 taaactggct cagaaggatc aagaaattgc gcaattacag agtcagatcc aaaactttga
    13021 tacagaaaaa gaattggcca agaaagaggt tgaacagaca agccatgagg ctctcttggc
    13081 taaggacaag gaagtacagc tcttagaaaa tcagttggct accttgcgtt tggagcatga
    13141 aaatcaactg caaaagaccc tttctgacct agaaaaagaa cgggatcagg ttaaaaacca
    13201 actacttttg caggaaaagg aaaatgaatt atgtttggct tctgttaagc aaaactacga
    13261 agcccagctc aaggcagcta gtgaacaagt cgagttttat aaggatttta aggctcaaca
    13321 atctacaaaa gcgattgggg aaagcctaga acagtatgca gagagtgagt ttaacaaggt
    13381 tcgtagtttc gcctttccaa atgcttactt tgagaaggat aacaaggtct cttcgcgtgg
    13441 gtctaaaggg gactttatct tccgtgagtg tgatgaaaat ggagttgaaa tcatttctat
    13501 catgtttgag atgaaaaacg aagcagacgg aacagagaag aagcacaaga atgcagattt
    13561 ttacaaggaa ttggacaagg accgtcggga gaagaactgt gagtatgccg ttttggtgac
    13621 catgcttgag gccgataatg actactttaa cacagggatt gttgacgtca gccacgagta
    13681 tgaaaaaatg tatgttgttc gtcctcaatt ctttatccag ttgattggtc tcttacgtaa
    13741 tgcggcgcta aattccctaa aatacaagca ggagttggcc ttggttcgcg agcaaaatat
    13801 tgacattacg cattttgagg aagacttgga tgcctttaag ctagcttttg ctaagaacta
    13861 taattcagct tcgactaact ttggaaaagc tattgatgaa atcgacaagg ccatcaaacg
    13921 catggaagag gttaagaaat tcctgactac atctgaaaac caactccgtc tagctaacaa
    13981 caaattggaa gatgtctctg ttaaaaaatt gacccggaaa aatccaacaa tgaaagcgaa
    14041 gttcgaagca ctgaaggggg agtagaaagc aaaaatgaac ggtattatca acttaaaaaa
    14101 agaagcagga atgacctcgc atgatgcggt ttttaaactg cgtaagattt tgggaaccaa
    14161 gaaaattggt catggtggaa ccttggatcc ggatgtggtg ggtgttttgc cgattgcggt
    14221 tggcaaggcg acacgcatgg tcgagtttat gcaggacgag ggtaagatct atgaggggga
    14281 aattactctg ggttattcca cgacgactga ggatgctagt ggggaagtga tcgcagaaac
    14341 ccctgttttg tctcccttgg atgaaaagct tgttgatgaa gcgattgcta gcttgactgg
    14401 gcctattacc cagattcccc ctatgtattc ggcagttaag gttaatggtc gcaagctcta
    14461 tgagtatgcg cgtgctggtc aggaagtgga gcgtccagaa cgtcaggtga tcatttatca
    14521 atttgagcga acaagtccga tttcttatga tggccaactt gcccgattca cttttcgtgt
    14581 aaaatgcagt aaagggactt acatccgtac tttgtcagtt gatttgggtg aaaagcttgg
    14641 ttatgcggct catatgtccc atttgactcg tactagtgct gctggcttac aattagaaga
    14701 cgctctttcc ttggaggaaa ttgctgaaaa agtagaggct gggcaattag attttctcca
    14761 tcctttagag attgggacag gtgaccttgt caaagttttc ctaactccag aagaggctac
    14821 agaagttcgc tttggtcgtt ttattgagct agaccaaacg gacaaagaac tggctgcctt
    14881 tgaagatgat aaattgttag ccattctaga aaaacggggc aatctctata agccaaggaa
    14941 ggtttttagc tagatcgttt aggaataaaa atcgggtgat agataacaat tgcttgataa
    15001 aaccccatac taatagtaga atggttttgg gaattataat attccaattg ttgcgagttg
    15061 taggtactca aataatctat atagaaattt agaggtgtga aatgaagcaa tttaaaattc
    15121 tttcagataa atatttagag tccattacag gttctggtgg gaacttaggc ccaggatttg
    15181 gtgtgataat tccatgatgc gaaatgagtt tcgagaaagg gtggagcaac ttcttcaaca
    15241 aaaagaaata aatgaaaata gtgagttgag tcacctgttt cgtcttgcta tacaaaattt
    15301 agacagaaat gaaaaatacc aatcggtcat ggccaatttg agtcaagggt tgtcacttta
    15361 cctcatgacg catcattacc aggcacctaa gtctgtcatt gattttggtt tatggattgc
    15421 caaagctcct agccaggaaa gagggagact ggctttcttg cagatgcttg ctcaaaccct
    15481 acagggcttt cgttaaaact gtcgaacatc agagggaaga ctctggtgtt ttttgctgat
    15541 gagactataa attttgtaaa aaatgtgata gaatagacga cggataaaat aacggaggat
    15601 agcatgcaaa atagaccaat cattattgga gtgacaggtg gttctggtgg tggtaaaacc
    15661 agtgtttcaa gagccatttt atcgcatttt cctgatgaaa agatttccat gattgagcat
    15721 gattcatact acaaggatca gtctcatttg acctttgaag agcgtgtcaa aaccaactat
    15781 gaccatcctt ttgcctttga tacagacttg atgatcgagc agattaagga attgttggca
    15841 gggcgtccgg tggacatccc gacctacgac tatacagagc atacacggag tagcaagacc
    15901 tatcgtcagg agcctcaaga tgtctttatc gttgagggca ttttggtctt ggaggacaag
    15961 cgtctgcgcg atttgatgga tatcaagatt tttgtggata cggatgacga tgtgcgtatt
    16021 attcgtcgta tcaagcgtga tatggaggaa cgtggccgta gccttgatag cgttattaac
    16081 cagtacttag gtgtggtcaa accaatgtac caccagttta tcgagtcaac taagcgttat
    16141 gctgatatcg tcattcctga aggggttagc aataccgtgg ctatcgacct gttgacgacc
    16201 aagattgcaa agattttgga agaagctcga aacagcaaat aatcacatgt ggaggccttg
    16261 cctccttttt ctatttttcc ctcataatgg tacataaatg gagattttta ggcatatttt
    16321 tggtataata atacccataa aagagcaagt aaatgaatag taggtggaga tggaaaagta
    16381 tttatcggta acaactttga ccaaatatct gaaaatgaaa ttcgataaag acccatactt
    16441 ggaacgggtc tatttaactg gtcaagtttc caactttcgt aaacgaccta ctcaccaata
    16501 tttctcccta aaggatgacc atgcagttat tcaagcgacc atctggtctg ggatttatca
    16561 gaaattaggg tttgacctgg aagaaggaat gaagatcaat gtgattgggc gtgtacaggt
    16621 ctatgaacca agtggtagct actccatcat cattgaaaaa gctgagcctg atggggttgg
    16681 ggcgcttgcg attcagtttg aacaacttaa gaaaaaattg acagaagaag gcctgtttca
    16741 agaacgcttc aagcaagctc taccccaatt ttctaagaga attggggtag taaccagccg
    16801 tagtggagcc gttattcgag atattatcac gaccgtcagc aggcgattcc caggtgttga
    16861 catccttctt tatcctacca aggttcaagg tgaaggggct gcggaggaaa ttgctagaaa
    16921 tattgcgcgt gctaatcaac gggacgattt ggatttgctc attattggtc gtggaggtgg
    16981 ttctattgag gatctctggg cctttaacga agaaattgtg gtacgagcta tttttgaatc
    17041 tcgtttgcca gttatctcta gtgtggggca tgagacggat gtgaccttgg cagattttgt
    17101 ggcagatcga cgcgctgcaa cgccaacagc ggcggctgaa ctggcaacac ctgtgaccaa
    17161 gttggatgta ttaactcatt tgcaaaatca ggaaaaacgg atggcaacgg cagtccgaaa
    17221 tgttctatct aagaaacaag aggctttgaa aaaatgcagt cagtctgtta tctttagaca
    17281 acctgagcgc ttgtatgacg gttatttgca acgcttggac caactgcaac tgcgtttgaa
    17341 acaaagtttg cgaactcgga tttctgataa caaacaatta gttcaagcaa gaactcatca
    17401 attagtacaa ttatcacctg ttaccaaaat ccaacgctat caagaccgtt taggacagtt
    17461 ggacaagctc ttaggtagcc aaatggcgtt agtttatgac gccaaggttg ctgaggccaa
    17521 gcgactttcg gaagctttgc tcatgttgga tactagccga atcgtggcgc gtggttatgc
    17581 tattgtcaaa aaagaagagt ccattgtaga ttcggttgag agtttgaaga aaaaagacca
    17641 agtaacgctt ttgatgcgag atggtcaagt agaattagag gttaaagatg tcaaaacaaa
    17701 agaaatttga ggaaaatcta gcagaactgg aaaccattgt ccaaagtttg gaaaatggtg
    17761 aaattgctct ggaagatgcg attactgcct ttcaaaaggg catggtcttg tcaaaagagc
    17821 tccaagctac gctggacaag gctgaaaaga ccttggtcaa ggtcatgcaa gaagacggaa
    17881 cagaaagtga ttttgaatga aaaagcaaga aaaattagct cttgtcgagt cggctttgga
    17941 agatttttat ggagaccagc agtttgcctc tagtttacgg gagtctgttc tctattctat
    18001 tcatgctggt ggcaagcgta ttcggccttt tctcttgtta gaagttctgg aagccttgca
    18061 ggttaccatc aaacctgctc acgcgcaggt agctactgcc ttggagatga ttcatacagg
    18121 gagcttgatt cacgatgacc ttcctgctat ggatgatgac gattatcgaa gagggcggtt
    18181 aaccaatcac aagaaattcg gtgaagctat ggccattttg gctggagatg ccttattctt
    18241 agacccatat gccttgattg cgcaggcaga tttgccaagt cagattaagg tggacttgat
    18301 tgccaactta tcccttgctt caggtagtct gggtatggtg gcagggcaag ttttggatat
    18361 ggagggcgaa caccagcact tgtctttgga agaacttcag attattcatg ccaataagac
    18421 tgggaagtta ctagcctatc ccttccaagc ggcagctatt atagctgaat tgtcacctga
    18481 aatgcaggtg aagctgaaaa ctgtgggtga attgattgga cttgcttttc aagtcagaga
    18541 tgatgtactg gatgtgacag ctagttttga ggaaatcggc aagacacctc aaaaggatct
    18601 gcaggcagaa aaatcaacct atcctgccct gttgggcttg gaagagtcca ttgccttttg
    18661 taaccaaacc ctggatcaag ctaatgaaaa attagaagaa attgcccagc agcttccctt
    18721 tgaaacagaa tcgattgtaa gtgtagtaga aagtttgaga atcaatggct aaggaaagag
    18781 tggatgtact agcttataaa caggggttgt ttgaaacgag agagcaggcc aagcgaggtg
    18841 taatggctgg cctagtcgta gcagtcctta atggagaacg gtttgacaag ccaggagaga
    18901 aaattccaga tgacaccgaa ttaaaactca agggagagaa actcaagtat gtcagccgtg
    18961 gtggtttgaa actggaaaag gccttgcagg tctttgattt gtcggtggat ggcgcgacta
    19021 cgattgatat cggggcctct actggaggtt ttaccgatgt catgctacag aatagtgcca
    19081 agttggtctt tgcagtcgat gttggtacca atcagttggc ttggaaatta cgccaagacc
    19141 cacgagttgt tagcatggag cagttcaatt tccgctatgc tgaaaagact gatttcgagc
    19201 aggagccgag ctttgccagt attgatgtga gtttcatttc ccttagtctg attttgccag
    19261 ccttgcatcg tgtcttggct gatcaaggtc aggtggtagc acttgtcaaa cctcagtttg
    19321 aggcaggacg tgagcagatt gggaaaaatg gaattattcg agatgctaag attcatcaga
    19381 atgtccttga atctgtaaca gctatggcag tagaggcagg tttttcagtc cttggcttgg
    19441 acttttctcc catccaaggt ggacatggaa atattgaatt tttagcgtat ttgaaaaaag
    19501 aaaagtcagc aagcaatcag atttttgctg agattaaaga agcagtagag agggcgcata
    19561 gtcaatttaa aaatgaataa aaaagagaga cttgaaaaaa ttagaagatt ggtgacagat
    19621 tatcaaatcg gcacgcaaga agaaattgta gaacatttga aagaagcagg tatcactgcc
    19681 actcaggcga cggtatcccg agatatcaaa gagttaggta ttgtcaaaat tcctttgaga
    19741 gacaacacct atgtctacga attaccaaaa tcaatcgtaa aaagtctgca actggctgaa
    19801 gacaatatcg aatcggctga attgatggat aagatgatca atctccaagt tattccagga
    19861 aatacggctt ttgtaaaagc tcagttaatc gatacttttg cagacaagat ttttagttgt
    19921 ttgactgatg atagctcgat tttagtcatt gccagaagtg gaagtctagc agaggaaatc
    19981 tttgaacaag taaaaaattg gtaggtctat atgttacttg aaatttcgat aaaaaacttt
    20041 gccattattg aggctatttc cctcaatttt gaaaagggga tgactgtcct gactggtgaa
    20101 acgggtgcag ggaagtcgat tatcattgat gccatgaata tgatgttggg agctcgtgcg
    20161 acgacagatg ttattcgtca tggtgcacca aaggcagaga ttgaggggct tttctcagtt
    20221 gagaatagtc gtcttttaca ggaaattttt gatgagcaag gtttagaatt aggtgatgaa
    20281 attatcatcc gtcgggaaat cttgcaaaat ggtcgtagta tcagccgtgt aaatggtcag
    20341 atggttaatc tgtctgtttt gcgagctatt gggcaacatc ttgtagatat tcatggtcag
    20401 catgaccatg aggagttaat gcgtccccaa ctgcatatcc agatgttgga tgaatttggt
    20461 gatgccgctt tttgggactt gaaagaaacc tatcaaacga gttttgatgc ctatcggaaa
    20521 atgcgcaagc aggttctgga agtcaagaaa aaccaacagg aacacaagga acgtatcgaa
    20581 atgttggaat ttcaaatggc agagattgag gcagcaaact tgcaggctgg agaagacttg
    20641 gctctcaatc aagagcgaga taaactcctc aaccacaaaa atattgcgga tacactgacc
    20701 aatgcctaca gtatgttgga caatgaagat ttttcaagtc tggccaatgt tcgttcagct
    20761 atgaatgaca tggaaagtgt cgaagagtat gatcctgaat accgtgaaat ttcaagctct
    20821 ctgtctgaga cctactatgt tttagaagac attagcaaac gtttggaagc tatcattgag
    20881 gaccttgatt ttgatggcaa tcgcctgatg caggttgaga atcgtttgga cctccttcat
    20941 actattactc gtaagtatgg tgggactgtt gatgatgttt tgctttattt tgccaagatt
    21001 acggaagaat acaatctctt gacaggcaat aatctttcgt ctgaggacat ggaagcagag
    21061 cttaagaagt tggaagtcaa tcttgtcaat ttggcaggtc aacttgcttc tgctcgtcat
    21121 aatttggctc agcaactcga agctgagatt aaacaagaac tgcaagatct ttatatggaa
    21181 aaagcccagt ttcaggttca ttttagtaag ggaaaattca gtcgtggggg aaatgaaatg
    21241 gttgagtttt atatttcaac caaccctgga gaagacttta aacccttggt taaggttgct
    21301 tctggagggg aattatctcg tctcatgtta gccattaagt ctgccttttc acgtaaagaa
    21361 ggcaagacta gcattgtctt tgatgaggtg gatacgggag tttcaggtcg tgttgctcaa
    21421 gctattgctc agaaaattca taaaattggt cagcatggtc aggttttggc tatctcccat
    21481 ttgccacaag taattgcgat tgcagattat caattcttta ttgagaagat tagtaatgac
    21541 cattcaacgg tttcgactgt tcgtctcttg acagtcgaag agcgagtgga ggaagttgcc
    21601 aagatgttgg caggtgatga tgtgacagaa gcagccctga cgcaagccag agaattgttg
    21661 agaaacaggg agaaataaga tgacagatta ttatgtaatt ggagatgttc acggaaaagc
    21721 tgggatgctg gaagaccttc ttaaaacctg ggatggtcag acccagttgc tctttctagg
    21781 tgatttgatt gacagaggtg aggatagtca ccgtgttctt gaaatggtca aggacttggt
    21841 ggacaatcaa ggggctatct gtttgtctgg aaaccacgag tatatgtttt tgacttggct
    21901 agatgaccca gaagaaagtt atgaccatta tcgtcgcaat ggtggagata caaccattaa
    21961 ctctatccta ggtcgtccct tggatgcacc agttgatgga gtagaagatg ccaagcgggt
    22021 tgccgctgaa gcgtcagact tggtcgaatt cattcgtcaa atgccatttg tagtagagac
    22081 agacaagtat atatttgttc acgcaggtat tgatttgact ttggacgact ggcatgaaac
    22141 aacagattat aaaaaagtat ggcttagaaa accattccac ggagctgaaa atcatactgg
    22201 aaaaaccatt gtcttcggtc atacaccggt ctatggtttg ttacagcaag aacgcggtac
    22261 atctgagctt tggactacag aagatggcaa gattggaatg gatggaggag ctgtttatgg
    22321 tggtgtcctt cacgggattg tctttacaga ccaaggaatg acagaacacc actttatcga
    22381 aaatgacggc tttgtcgctg aagattagta ctcctagcag ggtatggtct tgtcaaaata
    22441 tcaaaaacaa tttataataa atagataccc tgaaaggaag agaatcatga acttagaaga
    22501 attgaaaaaa cgacagggga agatccgtaa cttctctatt atcgcccata ttgaccacgg
    22561 gaaatcaact ctagcagacc gcattttgga aaaaacagag acggtttcaa gtcgtgaaat
    22621 gcaggcccag cttttggata gcatggatct agagcgggaa cgtggaatta ctattaagtt
    22681 gaatgccatc gagttgaatt acactgcaaa agatggggaa acttatattt tccacttgat
    22741 tgacacacca gggcacgttg actttaccta tgaagtttca cgttcgctag ctgcctgtga
    22801 gggtgctatt ttggtggtcg atgcggctca aggaattgag gctcaaactc ttgccaacgt
    22861 ttatctggct ttggataatg atttggaaat catgcctatc attaataaaa ttgacctgcc
    22921 ggctgcagat ccggagagcg tgcgtacaga gattgaagat gtgattggtt tggatgccag
    22981 tgaagcagtt ttggcttctg ccaaggctgg tattgggatt gaagaaatcc tcgagcaaat
    23041 tgtagaaaaa gtaccagcac caacgggtga tgtgacggcg ccacttaagg ccttgatttt
    23101 cgactctgtt tacgatgctt accgtggggt tatcctccaa gtgcgtgtca tggacggagt
    23161 ggtcaaacct ggtgataaga ttcagctcat gagcaatagt aagacctttg atgtggccga
    23221 agtcggtatt tttacaccaa aagcggttgg tcgtgatttc cttgcgactg gtgatgttgg
    23281 ttacattgcg gcgtctatca agacggttca ggatactcgt gtgggtgata ccgttacctt
    23341 ggcaaccaat cctgcggcag aaccattaca tggttataag cagatgaatc ctatggtctt
    23401 tgcgggtctc tacccaatcg aatcaaacaa gtacaatgac ctgcgtgaag cgcttgaaaa
    23461 attgcaactg aatgatgcta gtcttcagtt tgaaccagaa acatctcagg cacttggatt
    23521 tggtttccgt tgtggatttc ttggacttct ccatatggat gttatccagg aacgtttaga
    23581 gcgtgagttc aatattgatc tcatcatgac agctccgtct gttatttaca aagttaattt
    23641 gaccgacggt gagtctatgg atgtgtctaa cccatctgag tttccagacc caactaagat
    23701 tgcgaccatt gaagagcctt atgtcaaggc gcaaatcatg gtaccacagg agttcgtcgg
    23761 agcagtaatg gagctagctc agcgtaagcg tggggacttt gtgactatgg actatattga
    23821 tgataaccgt gtcaatgtta tctatcaaat tcctcttgct gaaatcgtct ttgacttctt
    23881 tgataaactt aagtcttcga cacgtggtta tgcaagtttt gactacgaat tgtcagaata
    23941 tcgcccatct aagctagtga aaatggatat ccttctcaac ggagacaagg tggatgccct
    24001 cagctttatc gttcataagg actttgctta tgaacgtggg aaactcatcg ttgataagct
    24061 caagaaaatc atccctcgcc aacaatttga ggttccaatc caagcggcta ttggacacaa
    24121 gattgtcgct cgtactgata tcaaggccct tcgtaagaac gtacttgcta aatgttatgg
    24181 tggtgacgtt tctcgtaagc gtaaactcct tgaaaaacaa aaagctggta agaaacgcat
    24241 gaaatccatc ggatcagttg aagttccgca agaagccttc ctcagcgtct tgagtatgga
    24301 tgaagaataa gttatcagaa atgcctttcc ttgttcacag gaaatttata taagaatcaa
    24361 tagtaaagca ggcttagatg gtctgctttt ttagttttta tactcttcga aaatctcttc
    24421 aaaccacgtc agcgtcgcct taccgtatgt atggttactg acttcgtcag tttcatctac
    24481 aa
//
LOCUS       DBEJAQ010000031        23980 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.031, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000031 DBEJAQ010000000
VERSION     DBEJAQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.031"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            80..365
                     /locus_tag="ACL297_001349"
                     /pseudo
     CDS             80..365
                     /locus_tag="ACL297_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654120.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
     gene            469..1257
                     /locus_tag="ACL297_001350"
     CDS             469..1257
                     /locus_tag="ACL297_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGR0071607.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPKMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            1288..1806
                     /gene="rimM"
                     /locus_tag="ACL297_001351"
     CDS             1288..1806
                     /gene="rimM"
                     /locus_tag="ACL297_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGR0071608.1"
                     /translation="MNYFNIGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            1796..2515
                     /gene="trmD"
                     /locus_tag="ACL297_001352"
     CDS             1796..2515
                     /gene="trmD"
                     /locus_tag="ACL297_001352"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGR0071609.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDDPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            2527..2865
                     /locus_tag="ACL297_001353"
     CDS             2527..2865
                     /locus_tag="ACL297_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HGR0071610.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(2896..3216)
                     /locus_tag="ACL297_001354"
     CDS             complement(2896..3216)
                     /locus_tag="ACL297_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071611.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            3314..3529
                     /locus_tag="ACL297_001355"
     CDS             3314..3529
                     /locus_tag="ACL297_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HGR0071612.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(3735..4271)
                     /locus_tag="ACL297_001356"
     CDS             complement(3735..4271)
                     /locus_tag="ACL297_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGR0071613.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            4697..5035
                     /locus_tag="ACL297_001357"
     CDS             4697..5035
                     /locus_tag="ACL297_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071614.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            5101..7083
                     /locus_tag="ACL297_001358"
     CDS             5101..7083
                     /locus_tag="ACL297_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071615.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVF
                     ILIYQYVQLFITLKQNEYVKKIILGLSKTYIAISSLKYFMMTITMVILFTFLMTGQIE
                     LLYIGAASLLVLMLSIIMSFRKLSESYTKILKGDES"
     gene            7080..7703
                     /locus_tag="ACL297_001359"
     CDS             7080..7703
                     /locus_tag="ACL297_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0071616.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPCKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            7700..8200
                     /locus_tag="ACL297_001360"
     CDS             7700..8200
                     /locus_tag="ACL297_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071617.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKELYKKIYES
                     FKEKNK"
     gene            complement(8366..9712)
                     /gene="gor"
                     /locus_tag="ACL297_001361"
     CDS             complement(8366..9712)
                     /gene="gor"
                     /locus_tag="ACL297_001361"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGR0071618.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            9977..11176
                     /locus_tag="ACL297_001362"
     CDS             9977..11176
                     /locus_tag="ACL297_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGR0071619.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            11160..11861
                     /locus_tag="ACL297_001363"
     CDS             11160..11861
                     /locus_tag="ACL297_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071620.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            11863..13122
                     /locus_tag="ACL297_001364"
     CDS             11863..13122
                     /locus_tag="ACL297_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071621.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            13246..15243
                     /gene="metG"
                     /locus_tag="ACL297_001365"
     CDS             13246..15243
                     /gene="metG"
                     /locus_tag="ACL297_001365"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGR0071622.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            15574..15894
                     /locus_tag="ACL297_001366"
     CDS             15574..15894
                     /locus_tag="ACL297_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGR0071623.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            15898..16713
                     /locus_tag="ACL297_001367"
     CDS             15898..16713
                     /locus_tag="ACL297_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071624.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGVFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            16788..17714
                     /locus_tag="ACL297_001368"
     CDS             16788..17714
                     /locus_tag="ACL297_001368"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGR0071625.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            17810..18046
                     /locus_tag="ACL297_001369"
     CDS             17810..18046
                     /locus_tag="ACL297_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGR0071626.1"
                     /translation="MTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTIEQNGLALEMT
                     PYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            18039..18737
                     /locus_tag="ACL297_001370"
     CDS             18039..18737
                     /locus_tag="ACL297_001370"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HGR0071627.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            18748..19227
                     /locus_tag="ACL297_001371"
     CDS             18748..19227
                     /locus_tag="ACL297_001371"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGR0071628.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            19235..19963
                     /locus_tag="ACL297_001372"
     CDS             19235..19963
                     /locus_tag="ACL297_001372"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0071629.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            20005..20874
                     /locus_tag="ACL297_001373"
     CDS             20005..20874
                     /locus_tag="ACL297_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HGR0071630.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            20849..21289
                     /locus_tag="ACL297_001374"
     CDS             20849..21289
                     /locus_tag="ACL297_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGR0071631.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            21412..23958
                     /locus_tag="ACL297_001375"
     CDS             21412..23958
                     /locus_tag="ACL297_001375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGR0071632.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
BASE COUNT         7585 a         4061 c         5168 g         7166 t
ORIGIN      
        1 ttttagaagt aaaggtgtac tattctagtt tcaatatact ataaaagatt gaacgtttag
       61 gcaattccaa gactacttaa tcgaacatta acagcagtct cggactgatg aaggtctctt
      121 tatgaagtta gtggaggaag tcggaaaagt agccgaggtt ttgaataggc gctctggtcg
      181 aaaagaaggg ataggattcg aacgaggaac ttgccagaga actggctgat atcatttact
      241 acaccgtcgc aatcgtagct attaatcata ttgaccttac caagactgtc tttgagaaag
      301 acaaaaccgc agccgttaat taccaacata aacataattt ggaaggatta ttgaagggaa
      361 agtagagatg ttgtcccctt cctccaactt gagaaaatcc ttggaacgtg ataaaataaa
      421 ggataatgat ttttaataat tcattatata gaatgagtgg atttctttat gaagaggttt
      481 gaagtatcta cagaaattgg tagcctctct gttacttatc aaaagcaaaa gaaagtgcta
      541 gtttgtctaa acggagcagg tttgctacca agttatgaaa atttttcact tatacttgaa
      601 aaacctcctc ctacaattgg ttatttgaca attgattttc cgaacacagg taggagtccg
      661 attcatgacc aagctggaaa aaatctagat aatcttgcag atgcggttta tgaagtactt
      721 gaagaattag gaatttctga atatatactt tgtgcacata gttggagtgg aattttagct
      781 tgcaaattac tcgagaaacc aattaagcgt cagactttag tagcaattga accgacaact
      841 aaaaaagtca tgtttgctga tttttcagaa aatccttatc caaaaatgga agagcagatg
      901 aggctgattg acgagtgtgg tcctgaactt tattttaaga acttaactca agcaacattt
      961 agtcctgaaa cgaataaaaa aatctgggaa ttaatgcaag aaaaaggctt agagttggaa
     1021 aatcaagatc cagaatttca gatatctgga gagattactg aggaagattt tgagaatttg
     1081 tcgatagaat ctcatgtccc tgtttttatt ttttgtcaga cttatagaga aaaagagtac
     1141 agagaatcag aatattggac ttccaatact aaactcattt taggagggaa tcaccattat
     1201 ttacaatggt cagaatcgga aaaaattgcg gctattattc gagaattgtc agaataagat
     1261 ggaaaaaagg agattacagg agacaagatg aactacttta atattgggaa aatcgttaat
     1321 acgcagggat tacagggtga gatgcgagtc ttgtctgtga cggattttgc agaagaacgg
     1381 tttaaaaaag gagctgagct ggctttgttt gatgaaaaag atcagtttgt ccaaacagtg
     1441 accatcgcta gccaccgtaa acagaagaac tttgacatta ttaaattcaa agatatgtac
     1501 catatcaata ctatcgaaaa gtacaaggga tacagtctca aggtcgctga ggaagatttg
     1561 aatgacctag acgatggtga attttactat cacgagatta tcggtttgga agtctatgag
     1621 ggtgatagct tggttggaac catcaaggaa atcctgcaac caggtgctaa tgatgtctgg
     1681 gtggtcaaac gaaaaggcaa acgtgatttg cttttacctt atatcccacc agtggttctc
     1741 aatgttgata ttccaaataa acgggtcgat gtggaaatct tagaagggtt agacgatgaa
     1801 gattgatatt ttaaccctct ttccagagat gttttctcca ctggagcact caatcgttgg
     1861 aaaggctcga gaaaaagggc tcttggatat ccagtatcat aattttcgag aaaatgctga
     1921 aaaggcccgt catgtagatg atgatcccta cggaggcggt cagggcatgt tgctcagagc
     1981 acaacctatt ttcgattcct ttgatgctat tgaaaagaaa aatccgcgcg ttattctcct
     2041 tgatcctgct ggaaagcagt ttgatcaggc ttatgctgaa gatttggctc aagaggaaga
     2101 gctaatcttt atctgtgggc actatgaggg ttatgatgag cgcattaaga ccttggtaac
     2161 agatgagatt tccctaggcg actatgtcct cactggtgga gaattggcag ctatgaccat
     2221 gattgatgct acagttcgcc tgattccaga agtgattggc aaggagtcta gccaccaaga
     2281 tgatagtttt tcttcaggtc ttttagaata tcctcagtac acacgtccct atgattatcg
     2341 aggcatggtc gtgccagatg tattgatgag tggccaccat gaaaagattc gtcagtggcg
     2401 attgtacgag agtttaaaga aaacctacga gcgcagaccg gatttacttg aacattatca
     2461 actgacagta gaagaagaaa aaatgctggc agaaatcaaa gaaaacaaag aataaaggag
     2521 aaacctatgc aagtaatcaa acgtaatggc gaaattgctg aatttaatcc agataagatt
     2581 taccaagcca tcttgaaggc agcccaaact gtttatgttt tgacagatga tttgcgtcaa
     2641 aatcttgctc aagtcactaa gaaggtagtt ttggatttac aagaagccaa ggtggaacgt
     2701 gcgactatca gtatgattca atctatggtt gaacatcgtt tattgggcgc aggttacatt
     2761 accattgcag aacactacat ttcctatcgt ttacaacgtg acttggaaag aagtggttat
     2821 ggagatcata tcgcagttca tttacatttt gaacaaattc gctaagaaaa aagagtggga
     2881 tgaagcaact acatctcact ctttcttaaa tctatttttt tgggctgttt ggacagttga
     2941 ggggacaaat cgcaggcgtt gaatatcgct tatacggata atccggctca catttttggc
     3001 aaaatttttc acgataattt gctggccttg ctgatcatat ttgatgatat cacctgtaaa
     3061 gcttgtctca gacaagataa ggtgaacagc ggtttgtttc tggatagcct cttcaatagt
     3121 ggctgtaagg gagttggttt cagtctggtc aggttggtca tgtccattta aaaaatcatg
     3181 tattttatct agaacttgct ggaatttatg tctcatattg gcctcccttc ttctatatca
     3241 atattatata aaattttcct aaaatctaca agattttacg aataaactaa tgaaactaaa
     3301 aaaggagaag aaaatggcag aatttacatt tgaaatcgaa gagcacttgt tgactctttc
     3361 tgaaaacgaa aaaggttgga ccaaggaaat taaccgtgtg agctttaatg gtgcccctgc
     3421 aaagtttgat attcgtgctt ggagtccaga ccatactaaa atgggcaaag ggattactct
     3481 ctcaaatgaa gaatttcaaa cgatggtgga tgcctttaaa ggcaactaat actcttcgaa
     3541 aatctcttca aaccgcatca gctttgcctt gccgtatgta tggttactga cttcgtcagt
     3601 ttcatccaca acctcaaaac agtgttttga gcagcctgcg gctagcttcc tagtttgctc
     3661 tttgattttc attgagtata agttttcaaa ataaaaaaaa aggatattga gtcatgataa
     3721 ctcgatatcc ttttttagtt agtaaagtaa gcctgatttt taaggcgttg aagtagggga
     3781 cggctaataa aactaatagc cagaagtttg ccaaggtctg gaatgataaa gggaagaacc
     3841 cccacaacaa gagctttttc aaatgccatt ccagctagga aatgcaagct gagaatcccg
     3901 ccgacaaaga caagggcatc acccaagagg tttgcaagaa aaatcttaac aacaccactc
     3961 ttgctgttgg ttagagagga agtaagtcca gagtaaacga gataaaacca aagatagcct
     4021 gcagtagggc caactaaagc ctgaaaacca gctccacctc ctgcaaagac aggaagaccg
     4081 atagcaccta gaagaagata gagtccagca gaaagtacag cctctctcgg tctaaagaca
     4141 gtagcaatca agccgattgc aaagttttgc agagtgaagg gaacaggtcc aattggaaga
     4201 ctgatttgtg ccaaaacagc aatgagagca gccccaatag cagggatagc ataaacgtga
     4261 gcttttttca aaatagttaa cctcttttct aatgtatagt aactattata ctcatatagg
     4321 agtcgatgtc aaccattttt taaaaacaag gtaacaaaaa ctgtaacagc cgattttcgt
     4381 gactagtcaa gttggataat ttttttgttg attcgaaaat tgtctgtata gataatccta
     4441 ttttattaat aaaaaatata ctagtaaata cttgacaaat taaaaaataa gtgttacatt
     4501 aaaaatgtaa acggatacat ttagatgtgt tcaattttaa aaggaggggt agttatgaga
     4561 tattttaacg actctagata ccataacggt gattaaaaac ctaaatataa tcaattcata
     4621 ttaaagtaat atggagggac taaaaagaga ctattattta atcttcatca aatatttaaa
     4681 ttcaaggaga agtaaaatga aaaaaagaat taataagaaa gcatctgttt ttctagttgc
     4741 aagtgttttc agttttagct ttgctactac agctagtgtc gttaacgcag taacggcaca
     4801 ccctgctcca agtcccatta ttgatgtatg ggaatatggt gtaaatgata actatggtta
     4861 ttcgaattac tttgttagtt ctccaagtaa tattggatca aaatcttcag taacgaattt
     4921 ttggggaaca gttaaagctc aggataaaaa agattatgga tgggcatatt caagtgcaac
     4981 aaaatcttgg aacgatgttc gcttaaatgc atattgggat tattatagat tttaaaaata
     5041 atttatgaaa acaagagacc caattataag attgggtctc ttgtaatatg gtaattgctt
     5101 atgaaattaa aattatgtat cattggattc ttcttttgtt tgattgctac aattggtctg
     5161 gtcactatta gtgataccga aatccccata cctttaccaa ttgatggagc tttttctatc
     5221 caaggaaaaa gtaatctttc taataacgaa atatacgaaa tggttcgtga cttatcaaaa
     5281 acggaaaagg ttaccattta caagccaatc gttcagagtt cgggtcagtt gaagtatgta
     5341 aattttgatg atgtaaataa tgaacaatta aagtcagcac caatagtagg gatgtattac
     5401 acacttggga aaatggatgt agacagtttg aaaccactta cgatgacggg actacaaaca
     5461 gtctatatgg cttatccttg gtatatagga ggaatattac aattcactgg aactttaaga
     5521 atactgttaa tgggttcaat ttatttaact ttattagttg ttttatttgt tgttaggacg
     5581 aggcagatca aagaaggagt gatacgacgt tctttgggct tgcctatata cgacttaaga
     5641 agagagtatg gcatttctct tatttttgaa ctgattatga tggctttatt gatgatttct
     5701 tacagttctt ttttgggtaa tggtttcttc acctatagtt ctaagttatt tttctctctg
     5761 cttttaacga attttatact atttcaaatc attgatctta ttacttttgt tttattttgg
     5821 ttgacgattc agattgaaaa gcctattgaa attattaaaa acaaggcaaa aaacaagctt
     5881 atttttgtcg tatggcttgc tattatttcg attatcatcc ttgtctcagg agttttttta
     5941 caagaaacaa agagtagtca atctagtatt aacatacaga tacagaattt agtaccatgg
     6001 gatacagtaa aagactggag aaggattgag ttccttggaa ttgaaagtaa ctctactaaa
     6061 aatagagaag ttaatgattc agatggtcag tatctacaga tagttgctgc cttaaaaaac
     6121 ttagatttct tatatataga acggtcttca gcatatgttc cagatttcat gaaaacttca
     6181 catgttatag aaaatttttc taaacaatta gaaaatgacg ggataacgaa tccagaaata
     6241 aataaagagt taatttatat caaccaaaca ggtgctaacc tacaaaataa agtgaatgga
     6301 acaaactatc atcttttaga taataagata gcaaccatct atattcctga gaagtggaaa
     6361 gaaaaccaga aatctattga gaatacagtt gtagcagaac aatttattgg aacaaactat
     6421 acaagagaac agcttgcagt tcaaataatt cctgatgggg aaaaaatttt ttattttaat
     6481 gaggatgctg ataataatct taaaatgaaa gatattttac cgctggcaaa tgtagcagat
     6541 agcaaagata atatagttgt tgtgttagat acggacaaaa tgatggaaaa taataagttt
     6601 tctttggcta gtaacatact atataaatct ctttttagtc ctgaagcggt aaaaaaaata
     6661 aatgagatga ctgtcctgtt gaatttctca atgaatccag tagatgttta ccaaattgtg
     6721 aaattaaaga ttcaatcctt agagcaccaa attttacttt cacaaatctt gcagaagata
     6781 atttacagta ttgtctttat actcatttat caatatgtcc aactttttat tactctaaaa
     6841 cagaatgaat atgtgaaaaa aatcattttg ggtctgtcaa aaacatatat agcaatttca
     6901 agtctcaaat attttatgat gactattaca atggttattc tatttacatt tcttatgaca
     6961 gggcaaatag agttgctata tattggagcg gcttctctgc tagttttgat gttgtcaatc
     7021 ataatgagtt ttagaaaatt atctgaaagc tacactaaaa ttttaaaagg agatgaatca
     7081 tgacaattga ccttttgaac gtttcaaaaa gtttcggctc aaagaagatc tttacagact
     7141 taaatttaat atttgaatct ggaaaaagct atgcattgat tggaggttct ggctccggta
     7201 aaagtaccct tcttaatatt ataggaagat tagaaaaaat tgacagtggg aatgttttag
     7261 ttgataaaca agatatttgg aagataaaag aaagaacttt ttttaaaaat actgttggat
     7321 atgtatttca gaattattct ttaatagata acaaaacagt gtatgataat ctgagcctta
     7381 tcactaaaga caaaaaaaca ataactgacg tacttgaaaa agtaggactg tcaagtgact
     7441 atttacacca aaaaatatat gaattgtctg gagggcaggc tcaacgtgta gctattgcca
     7501 gaatgttaat gaaaccatgt aaaattatct tagcagatga acctacagga gctttagatg
     7561 gtgagattgg aaaagaaatt attcgtttac tattgaatga aacagctgaa gataaatatg
     7621 tcattatagc gacacatgat ccagctgtct ataacgaagt tgatgtgata attgacatga
     7681 aagatatagg gtataaggta tgaaaaagaa aatttatatt gctttgatct ttgtaactgg
     7741 ggtactcgcc attttctttt ttggaaaaca aatgattaca aaagaaaata tcaataagcc
     7801 aacggtagag ttaacaatct atacgctttc ttctagtgat acagaaaaat ggaataaagt
     7861 gagacaagta gaaacggaag aagctatata cttcataact gtgaaagaag tatcatcttc
     7921 agaagaagta ttttcaaaca tcattgcaaa tggagctgct acggggttcg gagttcgtga
     7981 agaagaagtg aagaaattca ataatggttt aggagacacc atagaggact ctaagcataa
     8041 taaactaatt gaaatagaat tttttacttt ttctgatgat ggcgcgggat ttgtggtagc
     8101 aaactttgac tacggtaaag aagagttgaa ttctcagaaa aaagatataa aagaattata
     8161 taaaaaaata tatgaatctt ttaaagaaaa gaataaatga ttgtattata atccttgtat
     8221 tttgctatgg tttgttaatt gatttttgca tatcttactt caacatacta taaaataaaa
     8281 cataagaaaa cgagtatctc caactacgga aatactcgct ttttgatatt tagaagtgtt
     8341 ttttgctttg cttcttttgt ttcgattaac gcatggttac aaattcttca gatgcagttg
     8401 ggtgaattgc cacagttgca tcaaagtcag ccttggttgc tcccattttg atagcaacgg
     8461 caaatccttg aatcatttca tcaacgccgt agccaattcc atgaagtccg acaacttttt
     8521 cttctgaacc agctgttatg agtttgaaac gggattcttg acggttgcga gtgcaagcag
     8581 agtacataga tgcaaagctt gatttataaa ccttgatttg gtcttgaccg tattctttaa
     8641 tagcttgctc ttctgtcaaa ccaacagttc cgatagcagg gtgtgaaaag acaacagttg
     8701 gaatagttga gtaatccatt tttgcagtag tttttccgtt aaagagacgt tcagataggg
     8761 tacgtccggc cttgattgca actggagtca gttctttctc gcccgttaca tcacctagag
     8821 catagattcc ctcaacaaca gtattttggt attcatccac ttggataaag ccacgttcgt
     8881 tcagagtcac tccagctttt tcaagttgca agcccttaac gtttggacgg cgacctgtag
     8941 cccagataac ttggctagct gtgtgactag taccatcttc gaaatgaatg gtaatgccgt
     9001 cagtagtttt ttctaacttg acagggactt tgtgagtgtg aagtggtaag tttgttcttt
     9061 ccatttcctt gaccaaacct tcaacgatgt aggaatcaaa accacgtaaa ggacgatcgc
     9121 ggcgaacaaa gagatctgtc ttgacaccaa aagtgtggag tacgccagct aattcaacgg
     9181 cgatataacc agcgcctaga atggcaattg actctggaag ttcttcccag gcaaatacat
     9241 catcagaaga gccacctagc tcagcaccag gaatattagg aatacttgga tgggcacctg
     9301 tagcaatcac gatatgttta gcacgaatca gttcaccatt tacgcttaca gtatgagaat
     9361 ctacaaattc agcatgacct tcaatcaagt ctacaccgtt gcgtttaaaa ctaccatcat
     9421 aagaagaacg agcgcgatca atgtaggctt cacgattgcg acgtagggtt gcaaagtcaa
     9481 agttaagatc agtagtctta aagccgtagt cttctccaaa ttgatggaaa gtctcagcga
     9541 tttgcgcccc gtaccacatg atttttttag gaacacaacc gacgttgaca caggttccac
     9601 ctaatttctt ttcctcaata acggctgctt tggctccatg ttcaccagca cggttcatgg
     9661 tagcgattcc tccgctacct ccaccgatag caatgatatc atattctctc attgaaaact
     9721 cctttgtaat cttttaaata gtagagcgtc ttttttgata gttatatttt attctttttt
     9781 tgatttgatt gcaaaaatct gctacgcaca aaaaattgca aaaaagcttg ccaaataagt
     9841 caagatagtg tattatatat ctagtacaat agaaaagcaa aattccgaac gattgtttaa
     9901 tcctgaactg tattgtttgc tggagggaaa tttggtataa tagcaaagta ttgtaaaaaa
     9961 gaaaaggagt ttcattatga agaaaaagaa tggtaaagct aaaaagtggc aactgtatgc
    10021 agcaatcggt gctgcgagtg tagttgtatt gggtgctggg gggattttac tctttagaca
    10081 accttctcag actgctctaa aagatgagcc tactcatctt gttgttgcca aggaaggaag
    10141 cgtggcctcc tctgttttat tgtcagggac agtaacagca aaaaatgaac aatatgttta
    10201 ttttgatgct agtaagggtg atttagatga aatccttgtt tctgtgggcg ataaggtcag
    10261 cgaagggcag gctttagtca agtacagtag ttcagaagcg caggcggcct atgattcagc
    10321 tagtcgagca gtagctaagg cagatcgtca tatcaatgaa ctcaatcaag cacgaaatga
    10381 agccgcttca gctccggctc cacagttacc agcgccagta ggaggagaag atgcaacggt
    10441 gcaaagccca actccagtgg ctggaaattc tgttgcttct attgacgctc aattgggtga
    10501 tgcccgtgat gcgcgtgcag atgctgcagc gcaattaagc aaggctcaaa gtcaattgga
    10561 tgcaacaact gttctcagta ccctagaggg aactgtagtc gaagtcaata gcaatgtttc
    10621 taaatctcca acaggggcga gccaagttat ggttcatatt gtcagcaatg aaaatttaca
    10681 agtcaaggga gaattgtctg agtacaatct agccaacctt tctgtaggtc aagaagtaag
    10741 ctttacttct aaagtgtatc ctgataaaaa atggactggg aaattaagct atatttctga
    10801 ctatcctaaa aacaatggtg aagcagctag tccagcagcc gggaataata caggttctaa
    10861 atacccttat actattgatg tgacaggcga ggttggtgat ttgaaacagg gtttttctgt
    10921 caacattgag gttaaaagca aaactaaggc tattcttgtt cctgttagca gtctagtaat
    10981 ggatgatagt aaaaattatg tctggattgt ggatgaacaa caaaaggcta aaaaagttga
    11041 ggtttcattg ggaaatgctg acgcagaaaa tcaagaaatc acttctggtt taacgaatgg
    11101 tgctaaggtc atcagtaatc caacatcttc cttggaagaa ggaaaagagg tgaaggctga
    11161 tgaagcaact aattagtcta aaaaatatct tcagaagcta ccgtaatggt gaccaagaac
    11221 tgcaggttct caaaaatatc aatctagaag tgaatgaggg tgaatttgta gccatcatgg
    11281 gaccatctgg gtctggtaag tccactctga tgaatacgat tggcatgttg gatacaccaa
    11341 ccagtggaga atattatctt gaaggtcaag aagtggctgg gcttggtgaa aaacaactgg
    11401 ctaaggtccg taaccaacaa atcggttttg tctttcagca gttctttctt ctatcgaagc
    11461 tcaatgctct gcaaaatgta gaattgccct tgatttacgc aggagtttcg tcttcaaaac
    11521 gtcgcaagtt ggctgaggaa tatttagaca aggttgaatt gacagaacgt agtcaccatt
    11581 taccttcaga attatctggt ggtcaaaagc aacgtgtagc cattgcgcgt gccttggtaa
    11641 acaatccttc tattatccta gcggatgaac cgacaggagc cttggatacc aaaacaggta
    11701 accaaattat gcaattattg gttgatttga ataaagaagg aaaaaccatt atcatggtaa
    11761 cgcatgagcc tgagattgct gcctatgcta aacgtcagat tgtcattcgg gatggggtca
    11821 tttcgtctga cagtgctcag ttaggaaagg aggaaaacta agatgcagaa tctgaaattt
    11881 gccttttcat ctatcatggc tcacaagatg cgttctttgc ttactatgat tgggattatt
    11941 atcggtgttt catcagttgt tgtgattatg gctttgggtg attccctatc tcgtcaagtc
    12001 aataaagata tgactaaatc tcagaaaaat attagcgtct ttttctctcc taaaaaaagt
    12061 aaagatggtt cttttactca gaaacaatca tcttttacgg tttctggaaa ggaagaggaa
    12121 attcctgttg aaccgccaaa accgcaagaa tcctgggtcc aagaggcagc taaactgaag
    12181 ggagtggata gttactatgt aaccaattca acgaatgcca tcttgaccta tcaagataaa
    12241 aaggttgaga atgctaattt gacaggtgga aacagaactt acatggacgc tgttaagaat
    12301 gaaattattg caggtcgtag tctgagagag caagatttca aagagtttgc aagtgttatt
    12361 ttgctagatg aggaattgtc cattagttta tttgaatctc ctcaagaggc tattaacaag
    12421 gttgtagaag tcaatggatt tagttaccgg gtcattgggg tttatactag tccggaggct
    12481 aaaaggtcaa aaatatatgg gtttggtggc ttgcctatta ctaccaatat ctcccttgct
    12541 gcgaatttta atatagatga aatagctagt attgtctttc gagtgaatga taccagttta
    12601 accccaactc tgggtccaga actggcacga aaaatgacag agcttgcagg cttacaacag
    12661 ggagaatacc aggtggcaga tgagtccgtt gtatttgcag aaattcaaca atcgtttagt
    12721 tttatgacga cgattattag ttccatcgca gggatttctc tctttgttgg aggaactggt
    12781 gtcatgaaca tcatgctggt ttcggtgaca gagcgcactc gtgagattgg tcttcgtaag
    12841 gctttgggcg caacacgtgc caatatttta attcagtttt tgattgaatc catgattttg
    12901 accttgttag gtggcttaat tggcttgaca attgcaagtg gtttaactgc cttagcaggt
    12961 ttgttactgc aaggtttaat agaaggtata gaagttggag tatcaatccc agttgcccta
    13021 tttagtcttg cagtttcggc tagtgttggt atgatttttg gagtcttgcc agccaacaag
    13081 gcatcgaaac ttgatccaat tgaagccctt cgttatgaat gagatcaaca agatggacac
    13141 tcgtctatct tgtttttgta tggatttccc tatcgaaatt cattaaaaat gtgatataat
    13201 gaagtgttag aaaaacaggt ttcatttgcc tggaaaggaa aaattatgtc tgaaaaaaat
    13261 ttttatatta caacgccgat ttactatcca tctgggaaac ttcatatcgg ttctgcctac
    13321 acaactatcg catgtgatgt cctagcacgt tacaaacgcc tgatgggcta cgatgtattt
    13381 tatctgacag gtcttgatga acatggtcag aaaatccagc agaaagcgga agaagctggt
    13441 attacacctc aagcctatgt tgatggaatg gcggttggag ttaaagaact ctggcaatta
    13501 ctagatatct catacgataa atttatccgt acaactgatg actaccatga aaaagttgtc
    13561 gcacaagtct ttgaacgctt acttgctcaa gatgatatct acttgggtga atactctggt
    13621 tggtattcag tatcagacga agaattcttt acagaaagcc agctggcaga agttttccgt
    13681 gatgaagctg gaaatgtgac tggtggtatt gctccatcag gtcatgaggt tgaatgggtt
    13741 tctgaagaat catacttcct tcgccttagc aaataccaag accgtttggt cgaatttttc
    13801 aaagctcatc ctgaatttat cacgccagat ggtcgcctta atgaaatgct acgcaacttc
    13861 atcgagccag gtttggaaga tttggcggta tctcgtacaa cctttacatg gggagtgcct
    13921 gtcccatcaa atccaaaaca cgttgtctac gtttggattg atgcccttct taactatgcg
    13981 acagctcttg gctacgctca agacgaacat ggtaactttg acaagttctg gaatggaaca
    14041 gtcttccata tggtaggaaa agatatcctt cgcttccact ctatctactg gccaatcctt
    14101 cttatgatgt tggatgttaa attacctgat cgtttgattg cccatggttg gtttgtcatg
    14161 aaagacggaa aaatgtctaa gtcaaaaggg aatgtcgttt accctgaaat gttggtagag
    14221 cgttatggac tagatccact tcgttactac ctcatgcgta accttccagt tggttcagac
    14281 ggaaccttta ctcctgaaga ctatgtcggc cgtatcaact atgaattggc taatgacctt
    14341 gggaacctcc ttaaccgtac ggtttccatg attaataagt actttgatgg acaaatccct
    14401 gcctatgtag aaggtgtgac tgaatttgat catgttcttg ctgaggttgc agaacaatca
    14461 atcgcagact tccatacaca catggaagca gttgactatc cacgtgcgct tgaagcagtc
    14521 tggactctga tctctcgtac caataaatac atcgatgaga ctgcaccatg ggtcttggcc
    14581 aaggatgaag ctcttcgtga ccaattggca agtgtcatga gccacttggc agccagcatt
    14641 cgtgtagttg ctcacttgat tgaaccattt atgatggaaa ctagtcgtgc agttttgact
    14701 caacttggtt tggaagaagt ttctagtctt gaaaacttga gtttggctga cttcccagca
    14761 gatgtgactg tagttgccaa aggaacacct atctttccac gtctaaatat ggaagaagaa
    14821 atcgcctata tcaaggaaca aatggaaggc aataaaccag cagtcgaaaa agaatggaat
    14881 ccggacgaag ttgagctcaa actaaacaag gatgaaatca agtttgaaga ctttgacaag
    14941 gttgaaatcc gtgtcgcaga agtcaaagaa gtgtctaaag tagaaggttc agataagttg
    15001 cttcaattcc gcttggatgc tggtgatgga gaagatcgtc agattctttc aggaattgca
    15061 aaatactatc caaatgaaca agaattggtc ggcaagaaag ttcaaatcat tgctaacctc
    15121 aaaccacgta aaatgatgaa aaaatatgtc agccagggta tgattctctc agctgaacat
    15181 gatggcaaat taacccttct cacagttgat ccagctgtac caaatggaag tgtgattggg
    15241 taaaagtaga caggactagt tcaggctagt tctgtttttt tatttatact caatgaaaat
    15301 caaagagcaa actaggaagc tagccgcagg ttgctcaaaa cacagttttg aggttgtaga
    15361 tgaaactgac gaagtcagct caaaacacag ttttgaggtt gtagatgaaa ctgacgaagt
    15421 cagtaaccat acataaggta gggcgacgct gacgtggttt gaagagattt tcgaagagta
    15481 ttaactatta tgctttacaa actagtgtag aagtgatata ctataggtga agttactagt
    15541 aggtattaca aaatagatta gaagggaaaa gtgatgaaag aaactcagct attaaaaggt
    15601 gttcttgaag gttgtgtctt ggatatgatt ggtcaaaaag agcggtatgg ttatgagttg
    15661 gttcagactt tgcgagaggc tggatttgat actatcgttc caggaactat ttatcctttg
    15721 ttgcaaaagt tagaaaaaaa tcaatggata agaggcgaca tgcgcccgtc gccagatggt
    15781 ccagatcgga agtatttttc attaatgaaa gaaggagaag agcgtgtctc agtcttttgg
    15841 caacaatggg acgatttgag tcaaaaagta gaagggatta agaatggggg ttaaaccatg
    15901 aagaaaatga agtattacga agaaacaagc gctttgctac atgagttttc tgaggagaat
    15961 caaaagtatt ttgaggagtt gtgggaaagt tttaatcttg ctggatttct ctatgatgaa
    16021 gactatctca gagagcagat ctatttgatg atgctagatt tctcagaagc agaacgagat
    16081 ggcatgagtg cagaggatta tctaggtaag aatcctaaaa aaataatgaa agagattctc
    16141 aagggagcac ctcgcagttc tatcaaagag tcccttttga cgccaattct tgtcctggcg
    16201 gtattacgtt attatcaact actaagtgat ttttctaaag gtcctctatt aacagtcaat
    16261 ttgctcacat ttttagggca acttcttatt tttctgattg gatttggact tgtggccaca
    16321 attttacgaa gaagtttagt ccaagattct cctaaaatga aaattggcac ttacattgtt
    16381 gttgggacta tagttcttct agttgtttta ggatatgtag gaatggcaag cttcatacaa
    16441 gaaggagtct tttatattcc ggctccctgg gatagtttgt ctgtctttac gatttcgcta
    16501 gttatcggta tttggaattg gaaagaagcg gtctttcgtc catttgtcag tatgattatt
    16561 gcccatcttg tggtgggttc tctgctccgt tattatgagt ggatgggaat ttcaaatgtt
    16621 ttccttacaa aagttattcc tttagctgtc ctctttattg gaatctttgt cttgttccgt
    16681 gggtttaaga agataaaatg gagtgaagta tagtcaaaaa gccgttgcaa agcggttttt
    16741 tgttataatg aagggaagaa tgttagaaat taaaggagaa aaatttgatg agatacataa
    16801 ctcttggtca agatgacaaa gaattatcag aaattgttct cggaatgatg agaataaaag
    16861 ataaatctgt aaaagaagtt gaagagcttg tagaaacagc actttctgtt ggaatcaatg
    16921 ccttcgactt ggctgatatt tatggtcgtg gtcgttgtga agaactgtta ggtcttgtcc
    16981 taaaaaatcg tccagattta agagaaaaga tgtggattca gtccaaatgt ggcattcgca
    17041 ttgaagaatt tacctatttt gatttttcta aggactatat tataaaatca gtagacggta
    17101 ttttgcaaag attgaagatt gatcatctag atagcttgct ccttcatcga ccagatgctt
    17161 tgatggaatc tgaccaagta gcagaagcct ttgatctcct ttataaacaa ggtaaagttc
    17221 gagattttgg agtttctaat caaaatccta tgatgatgga gttgcttaaa aaagatgtca
    17281 agcagccgtt agctgttaat cagctacaat tgagtgcggc ttttactcca ggatttgaat
    17341 cagcttttca tgttaatatg gaagatagtc aagcagctat gcgagatggc agcatttttg
    17401 aatattgcca attacacgat gtggtcattc aagcatggtc tgtcttgcaa ttcgggtatt
    17461 ttaaagggaa ctttgttgga aatgagaaat ttcaagcttt aaatcaagta cttgatcgtt
    17521 tagctattaa atatggagta acctcttcaa ctattgccat ttcttggata ttgcgttatc
    17581 cagcaaaaat gcaggcagtc gtaggtacaa caaatcctaa gcacttgaga gaagttagcc
    17641 gagcggcaaa ctttagctta acaagaaaag aatggtatga aatttatctt gctgcaggga
    17701 ataatttgcc gtaagtagta gcaggtttga ataactcaca gtcaaaaagc cgtataaagt
    17761 ggttttttgc tataattaaa tgaagaatgt agaattaaag gagaaaaaca tgacatttga
    17821 agagatcctg cctgggttaa aggctaagag aaaatatgta cgaactggtt ggggaggtgc
    17881 ggaaaactat gtccaactct ttgataccat cgagcaaaat gggcttgcac ttgaaatgac
    17941 accttatttc ctaatcaacg tttctggaga aggagaaggt ttttccatgt ggagcccgac
    18001 agtttgtgat gttttggcaa cggattgggt agaagtgcat gactaaacgt gtactcatta
    18061 caggagtgag ttcagggatc ggattggctc aagctcgcct ctttttagag aagggctatc
    18121 aagtttatgg agttgaccaa ggtgaaaagc cactcttaga gggtgatttt cactttttac
    18181 agagagattt gaccttggac ttggaaccta tttttgactg gtgccctcag gtggatgttt
    18241 tgtgtaatac tgccggagtt ttggatgctt acaaaccact gttggaacaa acggcgcagg
    18301 acattcaaga gatttttgaa atcaactaca ttattccagt agagttgact cggtattatt
    18361 tgacacaaat gctggaaaat aaaaagggaa tcatcatcaa tatgtgttcc attgcttcta
    18421 gcctagcagg tggaggtggt cacgcctata cttcatccaa gcatgccttg gctggcttca
    18481 ccaagcagtt ggctctagac tatgctgaag ctaggattca ggtctttggt atcgctccag
    18541 gagcagtcaa gacagctatg accgctgcgg attttgagac aggtggctta gctgactggg
    18601 tggctagtga aaccccaatc aagcgctgga ttgaaccaga ggaagtggca gaagtcagtc
    18661 tctttttggc cagtggaaag gcatctgcca tgcagggaca aattctgaca atagatggtg
    18721 gctggtcttt gaagtaggag gagttggatg gaaatcaaaa atcactttgg ggtctactgt
    18781 gtttgctttg aaaatggaaa gttactttgc attgaaaaaa cgagaggccc ttatcaacat
    18841 cggtatgatc tacctggagg cagtcagcaa cttggtgaag gactgacgga aacgctgact
    18901 agagaagtta tggaagagac gggatttact gttagaagct actctaatcc tcgaatctac
    18961 gatgttttcg tcagggaaga gttaaaaaat tttatggttc accatgtcat agccttgtat
    19021 gatgttgaaa tgaatgagag tgcacctcaa gttacgattt cggaagctgt gtctgatggt
    19081 gcgaatgatt cacttggata tatttggatg gatattcaag agattacaga agaaaatgca
    19141 tcgccattag tcttgaaggt taagtctgaa ttattaggat tgccagaact ggacaagact
    19201 tcttacatga attggaaggt gaagtagagg gataatggaa ctatttaaaa catggaagaa
    19261 aaatatggtt ctctatggtc ttaaatctca aattggaact gtctaccgaa acaatgatag
    19321 gacaacaagc ttttatgatg ttgggaattt tctataccta gcaggggagt tggattccag
    19381 attttgggaa gattttgtta gaaaatatgg tttagattat aagattatta tttcagaaaa
    19441 tactaattgg caagattttc tgcatcgaaa agtggggcta aattctttta ctcgctattc
    19501 ttttaaagat aaggcaaatt ttcaagttga atttttaaat aatctagtta ctcatttaga
    19561 ggaaggttac aatattgtgc ctattgataa tcatatttat aactgctttt ctacggaaga
    19621 atggtcacag gatttacagg gggattttga gtcctatcag gattttgttt taaaaggtgg
    19681 atttggcttt gtgattctta aaaataatga actgattgct gggatttcct cagggttagt
    19741 ttaccgtaaa gcagttgaag tggaagttgc aactagacca aacgaacaag gaaatggatt
    19801 tgctaaaaag cttggtgctg caatgattct agagagttta aatagagata tgtttccact
    19861 ttgggatgct cataatgagg cttccaaaaa agtagcagaa tttttaggat atgagttatc
    19921 tgaaccttat gaagcttttg aactagagga aattttgata taattatata tgatattgta
    19981 tcttttaaga cgaggtgaat ggctatgaga aatcaacttg cccttagtgg cgaaaaaatt
    20041 cttgaaaaaa tttatcctca actatttcac cacgtcggca tgattcgtgg ggaatatttg
    20101 ttgagagagc ttaatcaaaa catcatatta gcaagttgtc agcaatttgt aaaagattat
    20161 ctagagacta tctgctccct gtactcagat gaagaagttt ggtatcgttt ttctgaatta
    20221 acgaatacag aagccaattg tttagtgggg actagagagt tttttgacga agggcatccc
    20281 ttgtttggct atagaggaac caggcgttta ctggcgtgtt tggatgaatt tcaggctgaa
    20341 gcacatgtcg ttacagaagt ttatcaaacc aatcccaatc tatctcttat ctttcctttt
    20401 gtcaatgatg ccgaccaatt aaaacaagct attacagtat tgcgtcagca gggttttact
    20461 gggaaagtcg gaacgatgat tgaattaccg tcagcgtatt ttgacttatc tagtatactg
    20521 gaaacgggca tttcaaagat tgtagttgga atgaatgatt tgacttcctt tgtttttgcg
    20581 actatgagaa acagtcaatg gcatgatatg gaaagtccaa taatgttaga tatgctaaga
    20641 gatatgcagg ataaagcaag aaagaacaag attgattttg ctgtagcagg ctatctgaat
    20701 acttctttca tacaaaaaat gaatcagttg ggtatcaagt gcattatcca ctacagttct
    20761 attccagaga tttttgattt agagattgac catccagatc atcttaaaca cataaaagaa
    20821 gaaagtaaaa aattacaaag gagtacccat gataccgcaa gaaatgtgga ataaatacaa
    20881 gcaaatcaat cctttgatcg gagatgaaat cgatgcttgg gcttttggag ttgaaccaga
    20941 tcttttagcg gatttggtgt ttaaaggaga aaagacagca acagcatcag cctatgacct
    21001 ctatgtacta gaagacgaac cacttcccca agtagggacc ttcgatatca ttttagatag
    21061 tcaaaatcag tctgtctgca ttgtcgaaat tacaaaggtt tctgttgaac tcttcaatca
    21121 agtttctgcg caacatgcct ttaaggaagg tgagggagac aaatcacttg cctattggcg
    21181 ccaggttcat gaggactttt tcacagactg tttgggtgaa gtagggctga cttttacacc
    21241 tgaaagcaag gttgttttag aagaatttcg caaggtctac ccactgtaga ctattagaag
    21301 gaagaaagtt ttggaaatcg ctgtccaatc cttttttttc aagcaaaata tgatataata
    21361 agtttgtttg aagaagagca gcagctctta aacttagaat aggagaaaac tatgcaagca
    21421 gttgaacatt ttattaagca atttgttcct gaacattatg atttattttt agatttgagt
    21481 cgtgagacca agactttttc tgggaaagtg accatcactg gtcaagcaca gagtgaccgc
    21541 atctccctcc accaaaaaga cttggaaatc acctctgtag aagttgcagg tcaagctcgt
    21601 ccatttacag ttgaccatga caatgaagcc cttcatatcg aattggctga ggctggtcaa
    21661 gttgaattgg ttcttgcctt ttctggtaaa attacagaca acatgacagg gatttaccct
    21721 tcttattata cagttgatgg agtcaagaag gaggtcttgt ctactcagtt cgagagccat
    21781 tttgcgcgcg aagctttccc atgtgtggat gagcctgaag ccaaagcaac ttttgacctc
    21841 tctcttcgct ttgaccaagc agaaggtgaa ttggccttgt caaacatgcc agaaatcgat
    21901 gttgaaaacc gtaaggaaac aggtatctgg aagtttgaga caacacctcg catgtcttct
    21961 tacttgttgg cctttgttgc tggtgatttg caaggggtga ccgctaaaac taaaaatggt
    22021 accctggtag gtgtctactc aaccaaagca catccacttt caaatcttga tttctcactg
    22081 gatatcgctg ttcgctctat cgagttttac gaagattact atggagttaa gtatccaatt
    22141 cctcaatctc tccacatcgc ccttcctgac ttctcagctg gtgctatgga aaactggggt
    22201 cttgtgacct accgtgaagt ttacttggtt gtcgatgaga actctacatt tgctagccgt
    22261 caacaagttg cccttgttgt ggcccatgaa ttggctcacc aatggtttgg gaacctcgtg
    22321 actatgaaat ggtgggatga cctttggctc aatgaaagtt tcgctaatat gatggaatac
    22381 gtctgtgtgg ataccatcga accaagctgg aatatctttg aagatttcca aacaggtgga
    22441 gtacctcttg ctcttgaacg tgacgctact gatggcgttc agtctgtcca cgtcgaagtt
    22501 aaacatccag atgaaatcaa tacactcttt gacggcgcta tcgtctatgc caaaggaagc
    22561 cgtctcatgc acatgcttcg ccgttggcta ggagatgctg attttgctaa aggtttgcat
    22621 gcctactttg aaaaacacca atacagcaac accattggta gtgacctttg ggatgccctt
    22681 ggtcaagcgt caggacgtga tgtcgcagcc ttcatggatt cttggttgga acagcctggt
    22741 tacccagttc ttactgtcaa agttgaaaat gatgtcttga agatttcaca aaaacaattc
    22801 ttcatcggtg agaacgaaga caagaaccgt ctctgggtgg tgcccctcaa tagcaactgg
    22861 aaaggcttgc ctgatacact cgaaactgaa agtatcgaaa tccctggcta cgcagctctt
    22921 cttgctgaaa atgaaggagc tcttcgtctc aacactgaaa atactgccca ctatattacc
    22981 gactatcaag gagacttgtt agaagctgtt cttgctgagc tagagacact tgataacaca
    23041 agcaaactgc aaattgttca agaacgtcgt ttgttggctg aggcagggca catttcttat
    23101 gcagacttgc ttccagttct tgataaactt gctaaggaag agtcttatct ggtggtttca
    23161 gctgtttctc aagtgatttc agcccttgag cgctttattg atgaaggaac ggatgctgaa
    23221 acagccttca aaggactggt tgctaaattg gctcgtcata actatgaccg tcttggtttt
    23281 gaagctaaag acggagaatc agatgaggat gaattggttc gtcagctggc tgtttcgatg
    23341 atgattcgct caaatgatgc agaagctcgt caagtcgcta gccaaatctt cgcaacacac
    23401 aaggagaatc ttgcagaact cccagcagct attcgttcac aagttctgat taatgagatg
    23461 aaacatcatg agactaaaga cttgttagca ctttatcttg atacttatac tcacgcaaca
    23521 gatgctgtct ttaaacgcca gttggcagca gctcttgcat acagtacaga tgcggataat
    23581 atccaaaact tgattacttc ttggaaggac aaatttgtgg tcaaaccaca ggacttgtct
    23641 gcttggtatt accaattcct agctcatcaa gcaactcaga aaacagcttg gtcttgggcg
    23701 cgtgaaaact gggcttggat taaggcagcc cttggaggag atatgagctt tgatagcttt
    23761 gttatccttc ctgctcatgt atttaagact cagcaacgct tggcggaata caaggagttc
    23821 tttgagccgc aactttctga ccttgctctt agccgtaaca tcggtatggg aatcaaggaa
    23881 attgcagcac gtgttgactt gattagccgt gaaaaagctg cagtcgaagc agttgttctt
    23941 caatacggaa atgcataaat aagcctaaaa taaaaagaaa
//
LOCUS       DBEJAQ010000032        23047 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.032, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000032 DBEJAQ010000000
VERSION     DBEJAQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.032"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            60..929
                     /locus_tag="ACL297_001376"
     CDS             60..929
                     /locus_tag="ACL297_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGR0071633.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            933..1889
                     /locus_tag="ACL297_001377"
     CDS             933..1889
                     /locus_tag="ACL297_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGR0071634.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1889..2653
                     /locus_tag="ACL297_001378"
     CDS             1889..2653
                     /locus_tag="ACL297_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071635.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2653..3363
                     /locus_tag="ACL297_001379"
     CDS             2653..3363
                     /locus_tag="ACL297_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071636.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3777..4433
                     /locus_tag="ACL297_001380"
     CDS             3777..4433
                     /locus_tag="ACL297_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGR0071637.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            4541..5636
                     /gene="prfB"
                     /locus_tag="ACL297_001381"
     CDS             join(4541..4612,4614..5636)
                     /gene="prfB"
                     /locus_tag="ACL297_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGR0071638.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5654..6346
                     /gene="ftsE"
                     /locus_tag="ACL297_001382"
     CDS             5654..6346
                     /gene="ftsE"
                     /locus_tag="ACL297_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGR0071639.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6339..7265
                     /gene="ftsX"
                     /locus_tag="ACL297_001383"
     CDS             6339..7265
                     /gene="ftsX"
                     /locus_tag="ACL297_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGR0071640.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            7551..9731
                     /locus_tag="ACL297_001384"
     CDS             7551..9731
                     /locus_tag="ACL297_001384"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HGR0071641.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            9783..10598
                     /locus_tag="ACL297_001385"
     CDS             9783..10598
                     /locus_tag="ACL297_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGR0071642.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            10738..12081
                     /locus_tag="ACL297_001386"
     CDS             10738..12081
                     /locus_tag="ACL297_001386"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGR0071643.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            12297..13487
                     /gene="metK"
                     /locus_tag="ACL297_001387"
     CDS             12297..13487
                     /gene="metK"
                     /locus_tag="ACL297_001387"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGR0071644.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSEEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIKK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            14040..14975
                     /locus_tag="ACL297_001388"
     CDS             14040..14975
                     /locus_tag="ACL297_001388"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HGR0071645.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            15009..16046
                     /gene="holA"
                     /locus_tag="ACL297_001389"
     CDS             15009..16046
                     /gene="holA"
                     /locus_tag="ACL297_001389"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGR0071646.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            16219..16824
                     /gene="sodA"
                     /locus_tag="ACL297_001390"
     CDS             16219..16824
                     /gene="sodA"
                     /locus_tag="ACL297_001390"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HGR0071647.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELTAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            16981..17511
                     /locus_tag="ACL297_001391"
     CDS             16981..17511
                     /locus_tag="ACL297_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HGR0071648.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            17532..18434
                     /gene="rlmN"
                     /locus_tag="ACL297_001392"
     CDS             17532..18434
                     /gene="rlmN"
                     /locus_tag="ACL297_001392"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGR0071649.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVSCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDID
                     AACGQLRFNTMKRDRQKAVAAVNP"
     gene            18434..18916
                     /locus_tag="ACL297_001393"
     CDS             18434..18916
                     /locus_tag="ACL297_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGR0071650.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQPL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            18918..20459
                     /locus_tag="ACL297_001394"
     CDS             18918..20459
                     /locus_tag="ACL297_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGR0071651.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(20515..21318)
                     /locus_tag="ACL297_001395"
     CDS             complement(20515..21318)
                     /locus_tag="ACL297_001395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGR0071652.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(21402..21615)
                     /locus_tag="ACL297_001396"
                     /pseudo
     CDS             complement(21402..21615)
                     /locus_tag="ACL297_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22094..22303)
                     /locus_tag="ACL297_001397"
     CDS             complement(22094..22303)
                     /locus_tag="ACL297_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071653.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            22473..22745
                     /gene="rpsP"
                     /locus_tag="ACL297_001398"
     CDS             22473..22745
                     /gene="rpsP"
                     /locus_tag="ACL297_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGR0071654.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            22765..23004
                     /gene="kphA"
                     /locus_tag="ACL297_001399"
     CDS             22765..23004
                     /gene="kphA"
                     /locus_tag="ACL297_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein KphA"
                     /protein_id="HGR0071655.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
BASE COUNT         6914 a         4162 c         5046 g         6925 t
ORIGIN      
        1 agaacctatc aaaaagtgag ggaaacccct cggaattata aatagaaaga gtgaatctta
       61 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
      121 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
      181 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
      241 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
      301 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
      361 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
      421 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
      481 ttttggctat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
      541 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
      601 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
      661 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
      721 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gcggctcttg
      781 gtggctttgt gattggtcta ttggaaacct ttgcgactgc ctttgggatg tcagatttcc
      841 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
      901 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
      961 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
     1021 tacttaatct attctatgta cagattttac agcaaattgg aattaatatt attttggctg
     1081 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
     1141 tggcgattgg tgcctatgca gcagctatta ttggttctaa attaccaacc tacggtgcct
     1201 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
     1261 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctgggt gtttctgaaa
     1321 ttatccgtat ctttatcatc aatggtggaa gccttacaaa tggtgcggca ggtatcttag
     1381 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
     1441 caaccttgaa cttcttgcgt agcccaattg gtcgttcaac cctctctgtt cgtgaagatg
     1501 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
     1561 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
     1621 taccgaaaga ttacaccttc atcaactcaa tcaacgtttt gattattgtt gtatttggtg
     1681 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttgaatatgc
     1741 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttggccttg gtattggtaa
     1801 tgattttcag accaggtgga ctccttggaa catgggaact gagcctatca cgtttcttta
     1861 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
     1921 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
     1981 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
     2041 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
     2101 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctctttaa
     2161 agatttaaca gttttagaca atgttttgat tgcttttgga aaccatcaca aacaacatgt
     2221 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
     2281 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
     2341 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
     2401 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
     2461 tgagttaatt cgtcgtatca aagatgaatt taagattaca atcatgttga ttgaacacga
     2521 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgaatatg gccgtttaat
     2581 tgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
     2641 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
     2701 caagcagtcc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
     2761 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgaccaagt
     2821 tcaggaaaga ttgaattttt aggtcaagaa atccaaaaaa tgccagctca gaaaattgtg
     2881 gcaggtggtc tttcacaagt tccagaagga cgccacgtct ttcctggctt gactgttatg
     2941 gaaaatcttg aaatgggagc tttcttaaag aaaaatcgtg aagaaaatca agctaacttg
     3001 aagaaggttt tctcacgctt tcctcgtctt gaagaacgta agaaccaaga tgcagctact
     3061 ctttcaggag gggaacaaca aatgcttgcc atgggacgcg ctcttatgtc aacaccaaaa
     3121 cttcttcttt tagatgaacc atcaatggga cttgccccaa tcttcatcca agagattttt
     3181 gatatcattc aagatattca gaagcaagga acaaccgtcc tcttgattga acaaaatgcc
     3241 aataaagcac ttgcaatctc tgaccgagga tatgtactgg aaacagggaa aatcgtccta
     3301 tcaggaacag gaaaagaact cgcctcatca gaagaagtca gaaaagcata tctaggtggc
     3361 taaaacaatc cagtggattg ttttagtcgg cagatgaaga ttacgaagta atcatcatta
     3421 tagtctggga gactagttta ggttgcagat aaagattgcg aagcaatcat caaatccagt
     3481 ggattgtttt agtcggcaga tggagattac gaagtaatca tcaatatagt ccgggggacc
     3541 tttttagtcg gtagattgag attgcaaaca aatctgcatc tacattgaaa gcttaatttc
     3601 taataattga aaaaatcgaa tgaaaaattt cttaccttca ttcacagagc tcgatttcag
     3661 agctcttttt gctagcttat tcatactttt ctgaatttcg aaaaagaaat gtaagcgttt
     3721 gatagattta caaaaagatt gtataatagg gataagaata gaaaaggaga agtctcatgg
     3781 cagttaaaga ttttatgacc cgcaaggtag tttatattag tccagataca acagtatctc
     3841 atgcagcaga tttgatgaga gagcaaggtt tgcaccgtct gcctgttatc gaaaatgatc
     3901 aattagttgg tttggtgact gagggaacca ttgcacaagc aagtccatct aaagcaacaa
     3961 gtctttctat ctatgagatg aattatcttc tgaataagac aaaagtaaaa gatgtcatga
     4021 ttcgcgatgt tgtcactgtc tcaggctatg ctagtctaga agatgcaact tatctgatgt
     4081 tgaaaaataa gattagtatt ctccctgtcg tagataacca tcaagtatac ggagttatta
     4141 ctgaccgtga cgttttccaa gcctttcttg aaattgcagg ttatggcgaa gaagggattc
     4201 gtgtacgctt tgttacagaa gatgaagttg gtgttcttgg aaaaattgtt tctttgattg
     4261 tagaagaaaa tttgaatatc tcccatacag tcaatattcc gcgtaaggat ggtaaggtga
     4321 ttatcgaagt gcaaatcgat ggatcaattg atttaccagc cttgaaagaa aaatttgaag
     4381 caaatggtat tcaagtggaa gaaatcgctc gcacttcagc aaaagtcttg taagaaggga
     4441 agcccaaagg cttctttttt catgaaaagg ggattagaga aaaagatgga aagaaatgat
     4501 aaaatatgct ataatgaaat aatgtaaaaa aggagtattt atggacattt cagtaattcg
     4561 tcagaaaatt gacgcaaatc gtgaaaaatt agcttctttc agggggtctc tttgacctcg
     4621 aagggctaga ggaggagatt gccatcttgg aaaacaagat gacagaacct gatttttgga
     4681 acgataatat tgcagcccaa aaaacgtcgc aagaattaaa tgaattaaaa aacacttaca
     4741 ataccttcca taagatggaa gagttgcagg atgaagtcga aattttattg gattttttgg
     4801 ctgaagacga gtcagtgcat gatgaactgg tagcgcagtt agccgaactt gataagataa
     4861 tgaccagcta cgagatgact ctactcttgt cagaacctta tgaccacaac aatgccatct
     4921 tggaaatcca tccaggttct ggtggtactg aggcgcagga ctggggtgat atgttgcttc
     4981 gtatgtatac tcgttatggt aatgctaaag gctttaaagt ggaagtgttg gattaccaag
     5041 caggtgatga ggctggtatt aagtcggtaa ctttatcatt tgaagggcct aatgcctatg
     5101 gtctcctcaa gtcagaaatg ggtgttcacc gcttagtgcg aatctcacca tttgactctg
     5161 ccaaacgtcg ccatacctct ttcacatctg tagaagtgat gccagaattg gatgatacta
     5221 ttgaagtgga aatccgtgaa gatgatatca agatggatac cttccgttca ggtggtgccg
     5281 gtggacaaaa cgtcaataag gtttcaacag gtgtacgttt aacccacatt ccaactggaa
     5341 ttgttgtcca atcaacagtg gatcgtaccc agtatggaaa tagagatcgt gccatgaaga
     5401 tgttgcaggc taagctctat caaatggagc aagagaagaa ggctgcagag gtagattctc
     5461 tcaaaggtga gaaaaaggag atcacttggg gaagccaaat ccgttcttat gtcttcacgc
     5521 cttatactat ggtaaaagat caccgaacta gctttgaggt tgctcaggta gataaggtta
     5581 tggatgggga cttagatggt tttatcgatg cttatctcaa gtggcgaatt agctaagata
     5641 gaaaggaact cacatgtcaa ttattgaaat gagagatgtc gttaaaaaat acgacaacgg
     5701 aacgactgct ctacgcggtg tttcggttag cgttcaaccg ggggaatttg cttacatcgt
     5761 aggaccttca ggagcaggga agtcaacttt tattcgttct ctgtatcgtg aagtaaaaat
     5821 cgataaagga agcctatcag ttgctggttt taatctggtt aagatcaaaa agaaagatat
     5881 cccgcttcta cgtcgtagtg ttggggttgt cttccaggat tataaattgt taccaaagaa
     5941 aactgtctat gaaaatattg cttacgctat ggaagtaatc ggggaaaatc gccgtaatat
     6001 caaaagacga gtgatggaag ttttggactt ggttggattg aagcataagg ttcgttcttt
     6061 cccaaatgaa ctctcaggtg gggagcaaca gcggattgcg attgcgcgtg caattgtaaa
     6121 taatcccaaa gtattgatag ctgatgagcc aacaggaaat ctggatccgg ataattcatg
     6181 ggaaattatg aatcttttgg aacggattaa cctacaagga acaactattt tgatggcgac
     6241 tcataatagc cagattgtaa ataccttgcg ccaccgtgtc attgccattg aaaatggccg
     6301 tgtcgttcgt gacgaatcaa aaggagagta tggatacgat gattagtaga ttttttcgcc
     6361 atttatttga agccttaaaa agtttgaaac gaaatggttg gatgacagta gctgctgtca
     6421 gttcagtcat gattactttg accttggtgg caatatttgc atctgttatt ttcaatacag
     6481 cgaaactagc tacagatatt gaaaataatg tccgtgtagt agtttatatc cgaaaggatg
     6541 tggaagataa tagtcagaca attgaaaaag aaggtcaaac tgttacaaat aatgactacc
     6601 acaaggtata tgattctttg aagaacatgt ctacggttaa aagtgttacc ttttcaagta
     6661 aagaagaaca atatgaaaaa ttaaccgaga taatgggaga taactggaaa atctttgaag
     6721 gagatgccaa tcctctctat gatgcctata ttgtagaggc aaacactcca aatgatgtaa
     6781 aaactatagc cgaagatgct aaaaaaattg aaggtgtctc tgaggttcaa gatggcggtg
     6841 ccaatacaga aagactcttc aagttagctt catttatccg tgtttgggga ctagggattg
     6901 ctgctttgtt aatttttatc gcagttttct tgatttcaaa taccattcgt attaccatta
     6961 tttcccgcag tcgcgaaatt caaatcatgc gcttggtcgg agctaaaaac agttatatcc
     7021 gtggaccgtt cttgttagaa ggagccttta tcggtttatt gggagctatc gcaccatctg
     7081 ttttggtctt tattgtttat caaattgttt accaatctgt caacaaatcg ttggtagggc
     7141 aaaatctatc catgattagt ccagatttat ttagtccgtt gatgattgcc ctactatttg
     7201 tgattggggt tttcattggt tcattgggat caggaatatc catgcgccga ttcttgaagg
     7261 tttaggtaaa atagctgctt ttatgaggag attgtaaaat ctcctttttt gctacaagag
     7321 tttttgaaaa gagatgcgca gaagaaaaga gcttccaaag aagtccccca gagaagactt
     7381 cttgatggct aatttgaact tgaaatttgg cgcactagaa attttttaga aaaaagtgta
     7441 atttttttat aaaagccttt acaaaaaaaa ttaaagtggt ataattaaat catagaaagt
     7501 gcaaacgttt tcgtaaaacg tttgcctaaa taaaaataaa ggagatttga atgatgaaag
     7561 atacattcaa aaatgtcttg tctttcgaat tttggcaaaa attcggtaag gctttgatgg
     7621 tagttatcgc ggttatgccg gctgctggtt tgatgatttc aatcggtaag tctatcgtga
     7681 tgattaaccc aacctttgca ccacttgtca tcacaggtgg aattcttgag caaatcggtt
     7741 ggggggttat cggtaacctt cacattttgt ttgccctagc cattggagga agctgggcta
     7801 aagaacgtgc tggtggtgct ttcgccgctg gtcttgcctt catcttgatt aaccgtatca
     7861 ctggtacaat ctttggtgta tcaggcgata tgttgaaaaa tccagatgct atggtaacta
     7921 ctttctttgg tggttcaatc aaagttgctg attactttat cagtgttctt gaagctccag
     7981 ccttgaacat gggggtattc gtagggatta tctcaggttt tgtaggggca actgcttaca
     8041 acaaatacta caacttccgt aaacttcctg atgcactttc attcttcaac gggaaacgtt
     8101 tcgtaccatt tgtagttatt cttcgttcag caatcgctgc aattctactt gctgctttct
     8161 ggccagtagt tcaaacaggt atcaataact tcggtatctg gattgccaac tcacaagaaa
     8221 ctgctccaat tcttgcacca ttcttgtatg gtactttgga acgtttgctc ttgccatttg
     8281 gtcttcacca catgttgact atcccaatga actacacagc tcttggtggt acttatgaca
     8341 ttttaactgg tgcagctaaa ggtactcaag tattcggtca agacccacta tggcttgcat
     8401 gggtaacaga ccttgtaaac cttaaaggta ctgatgctag tcaatatcaa cacttgttag
     8461 atacagtaca tccagctcgt ttcaaagttg gacaaatgat cggttcattc ggtatcttga
     8521 tgggtgtgat tgttgctatc taccgtaatg ttgatgctga caagaaacat aaatacaaag
     8581 gtatgatgat tgcaacagct cttgcaacat tcttgacagg ggttactgaa ccaatcgaat
     8641 acatgttcat gttcatcgca acacctatgt atcttgttta ctcacttgtt caaggtgctg
     8701 ccttcgctat ggctgacgtc gtaaacctac gtatgcactc attcggttca atcgagttct
     8761 tgactcgtac acctattgca atcagtgctg gtattggtat ggatatcgtt aacttcgttt
     8821 gggtaactgt tctctttgct gtaatcatgt actttatcgc aaacttcatg attcaaaaat
     8881 tcaactacgc aactccaggg cgcaacggaa actacgaaac tgctgaaggt tcagaagaaa
     8941 ccagcagcga agtgaaagtt gcagcaggct ctcaagctgt aaacattatc aaccttcttg
     9001 gtggacgtgt aaacatcgtt gatgttgatg catgtatgac tcgtcttcgt gtaactgtta
     9061 aagatgcaga taaagtagga aatgcagagc aatggaaagc agaaggagct atgggtcttg
     9121 tgatgaaagg acaaggggtt caagctatct acggtccaaa agctgacatt ttgaaatctg
     9181 atatccaaga tatccttgat tcaggtgaaa tcattcctga aactcttcca agccaaatga
     9241 ctgaagcaca acaaaacact gttcacttca aagatcttac tgaggaagtt tactcagtag
     9301 cagacggtca agttgttgct ttggaacaag taaaggatcc agtatttgct caaaaaatga
     9361 tgggtgatgg atttgcagta gaacctgcaa atggaaacat tgtatctcca gtttcaggta
     9421 ctgtgtcaag catcttccca acaaaacatg cttttggtat tgtgacggaa gcaggtcttg
     9481 aagtattggt tcacattggt ttggacacag taagtcttga aggtaaacca tttacagttc
     9541 atgttgctga aggacaaaaa gttgcagcag gagatctcct tgtcacagct gacttggatg
     9601 ctatccgtgc agcaggacgt gaaacttcaa cagtagttgt cttcacaaat ggtgatgcaa
     9661 ttaaatcagt taaattagaa aaaacaggtt ctcttgcagc taaaacagca gttgctaaag
     9721 tagaattgta atatacttga ggttggaagc tgtattccaa cctcttattt tgggagaaaa
     9781 gaatgaaatt tttaacactc aatactcaca gttggatgga gaaagaagca gaggaaaaat
     9841 tccagatttt gcttgaagat attcttgaaa aggactatga tttgatttgt ttccaagaaa
     9901 tcaatcagga gatgacctcg tcagaggtgg aggttaatga cctttatcaa gctttgccag
     9961 cagctgagcc tattcaccaa gaccattatg ttagactctt ggttgaaaag ttgttagagc
    10021 aagggaaaaa ttactactgg acctgggctt ataaccatat cggctatgac cgctaccacg
    10081 aaggtgtggc tatcttgtct aaaacaccta ttgaagccag agaaattttg gtttcagatg
    10141 tggatgatcc aacagactat catactcgcc gtgttgccct agctgaaact gtagtcgatg
    10201 gcaaggagct agcagttgcc agtgttcatc tctcttggtg ggataaaggt ttccaagaag
    10261 aatgggcacg atttgaggct gtcttgaaaa aattgaacaa gccactttta ctagctggag
    10321 attttaacaa cccagcaggt caggaaggtt accaagctat tttagctagt ccattaggct
    10381 tacaagacgc atttgaagtt gctcaagaga aaagtggtag ctatactgtt ccgcctgaaa
    10441 ttgatggctg gaaagggaac actgaacccc ttcgaatcga ttatgtcttt actaccaaag
    10501 agttagcggt ggaaaattta catgtcgtat ttgatggtaa caagagtcca caagtgagtg
    10561 atcactatgg cttgaatgct atattaaact ggaaataata actgaaaaga ggttggaact
    10621 ataaaattcc agccttttct tactagagaa gctactggaa atagcctaaa taagtgagac
    10681 tactgtaatg gaataaaata tggtataatt gataaggtag atagaatcga ggatgttatg
    10741 tcatttacga aatttcaatt taaaaactat attagagaag ccttgaagga gttaaaattt
    10801 acaactccaa cagaggtgca agacaagttg attcctattg ttttggcagg tcgtgaccta
    10861 gtaggagaat caaaaacagg ttcaggtaag actcatactt tcttgttacc gattttccag
    10921 caattagatg aagctagcga tagtgtacaa gcagtgatta ctgcaccgag tcgtgagttg
    10981 gctactcaaa tttaccaagt agcgcgtcag atttcagctc actcagatgt cgaagttcgt
    11041 gtggttaatt atgtgggtgg tacggataag gctcgccaga ttgagaaatt ggcaagcaat
    11101 cagcctcata ttgttattgg aacaccaggc cgtatctacg acttggttaa atctggtgat
    11161 ttagctattc ataaagccaa gacatttgtt gttgatgaag cagatatgac cttggatatg
    11221 ggattcttgg aaactgttga taagattgct ggcagtcttc caaaagactt gcaattcatg
    11281 gtcttctcag cgactatccc acaaaaactg caaccattct tgaaaaaata cttatcaaat
    11341 cctgttatgg agaaaattaa gaccaaaacg gttatttctg acaccattga taattggttg
    11401 atttcgacca agggacatga taagaatgct caaatttacc agttgactca gttgatgcag
    11461 ccgtatttgg caatgatttt tgttaacact aaaacacgtg ctgatgaatt gcattcatat
    11521 ctgactgctc aaggcttgaa ggttgcaaaa atccatggag atattgcccc tcgtgaacgc
    11581 aagcgaatca tgaatcaggt gcaaaatctg gattttgagt atattgtcgc aacagatttg
    11641 gcagcgcgtg ggattgacat tgaaggtgtc agccatgtca tcaatgatgc cattccgcaa
    11701 gacttatctt tttttgttca tcgtgttggt cgtactggac gaaatggcct accaggtaca
    11761 gctattaccc tttatcagcc aagtgatgac tcggatatcc gtgagttgga gaaattggga
    11821 atcaagttta gtcctaagat ggtcaaagac ggggaatttc aagataccta tgaccgtgat
    11881 cgtcgtgcca accgtgagaa aaaacaagat aaacttgata tcgaaatgat tggtttggtt
    11941 aaaaagaaaa agaaaaaagt caaaccgggt tataagaaga aaattcaatg ggcggttgat
    12001 gaaaagcgcc gtaaaaccaa gcgtgctgaa aatcgcgctc gcggtcgtgc agagcgtaaa
    12061 gctaaacgcc aaacatttta atagaaattg ttggagtatt gagctccaac ttttttattt
    12121 atgagaacga actatctaaa ccgaaacact acattaaaga ctgcaaattg cgattaaaaa
    12181 tggtataatg ataaagttat atagtcccga taagatggta ggtatttatt acgaagagtt
    12241 ttcctatcag tactttgtaa ctctataaca atatttttta agggggggac atttttatgt
    12301 cagagcgtaa attattcacg tctgaatctg tatctgaggg gcatccggat aagattgcag
    12361 accaaatttc agatgcgatt ttggatgcta ttttagcaaa ggatccagag gcgcacgttg
    12421 ctgctgaaac agctgtatat actggttctg tccacgtttt tggtgaaatt tctacaaatg
    12481 cctatgtgga tattaaccgt gtggttcgtg ataccattgc agagattggt tataccaata
    12541 cagaatatgg attttctgct gagacggtgg gagtacaccc atctttggtg gaacaatctc
    12601 ctgacatcgc tcaaggtgtt aacgaagcct tggaggttcg tggaaatgct gatcaagatc
    12661 cactggactt gattggagca ggtgaccaag ggctcatgtt tggatttgca gtagatgaaa
    12721 cagaagagct catgccattg ccaattgcac tcagtcataa attggttcgt cgtctggcag
    12781 aacttcgtaa gtctgaagaa attagctatc tccgtccaga tgcaaaatca caagttacag
    12841 ttgagtacga tgaaaatgac cgtccggtac gtgtagatac agtcgttatt tctactcagc
    12901 atgatccaga ggccactaat gaacaaatcc atcaagatgt gattaaaaag gtcatcaaag
    12961 aagttattcc atcttcttat cttgatgata agacaaaatt ctttatcaat ccgacaggtc
    13021 gttttgtaat cggtggtcct caaggggact caggtttgac tggtcgtaag attattgtag
    13081 atacttatgg tggctactct cgtcatggtg gtggtgcctt ctctggtaaa gatgcgacta
    13141 aggtggatcg ttcagcctct tatgcggctc gctatattgc caagaatatc gttgcagcag
    13201 accttgctaa gaaggcagaa gtgcagttgg cctatgctat cggtgttgcg caacctgttt
    13261 ctgttcgtat cgatactttc ggtacaggaa cagtagctga aagtcaactt gaaaaagcgg
    13321 ctcgtcaaat ctttgacctt cgccctgcag ggattatcca aatgctggac ctcaagcgtc
    13381 caatttaccg tcaaacatcg gcttacggtc acatgggacg tacagatatt gatcttccat
    13441 gggaacgttt ggataaggta gatgctttga aagaagcagt aaaataagat tttaagaggg
    13501 gaacgtcctc tcttttttat agtttttaac tatactggga tactgttctg aaaatccatt
    13561 ttgtgaaagt agagatttac atgtatagta gattgaaact agaatagtac acctcaactt
    13621 ctaaaacatt gttagaaatc gatttgactg tcctgattga tttctcctgt tcttgtttca
    13681 ttttactata tttctttaaa aatgataaag gttgagattt ctcctcgtaa tagataatca
    13741 tggggatatt tcaatccaaa gttttattcg ttatcacttg actattgcaa ggttttctag
    13801 agcaacagag tcatggaatg gactcatggt tgagatttct ccttgttgct tggacttcat
    13861 tcaaaagtct gttacccaag ccttgttcaa acttccaata cactagctgt ttccatagca
    13921 tgacttctgt actagacttt cttttccgaa taaatagata gaaccacaga atctagtaaa
    13981 cctagaatta aaattatggt ataatattag caataaaaga aatctggagg attagaatca
    14041 tggtatcaac gaaaacacaa attgctggtt ttgagtttga caattgcttg atgaatgcag
    14101 caggtgtggc ttgtatgacg atagaggagt tagaagaggt caaaaactca gcggcaggaa
    14161 cctttgttac taagacagcg accttggact tccgtcaggg gaatcctgag ccacgctacc
    14221 aagatgttcc acttggttcc atcaactcta tgggcttgcc aaataatggc ttagactatt
    14281 atttggatta tcttttagat ttgcaggaaa aagagtcgaa ccgaactttc ttcttatctc
    14341 tggtcggcat gtctccagag gaaacccata ctattttgaa aaaagtccaa gagagtgatt
    14401 ttcgtggtct gactgagcta aatctttcct gtccaaatgt tccaggtaaa cctcagattg
    14461 cctatgattt tgagacaaca gaccggattt tggcagaagt gtttgcttac ttcaccaaac
    14521 ctcttggaat taaattgcca ccttattttg atattgttca ctttgatcaa gcggcagcta
    14581 ttttcaacaa atatccgctc aagtttgtca actgcgttaa ctctatcgga aacggcctct
    14641 atatagaaga cgaatctgtc gttattcggc ctaagaatgg ttttggtgga attggtggag
    14701 aatacatcaa accgactgct ctagccaatg ttcacgcctt ttatcaacgt ttaaatcctc
    14761 aaatccaaat tatcggaaca ggtggcgttc tgactggtcg agatgccttt gaacacatcc
    14821 tctgtggagc aagtatggtg caggtgggaa cgacccttca caaagaaggt gtcagtgctt
    14881 ttgaccgcat taccaatgaa ctgaaagcaa tcatggtgga aaaaggctac gagagcttag
    14941 aagatttccg tgggaaattg cgctatattg actaaattaa atcgaaaaat ctgaagaaag
    15001 gagagacgat gctagccatt gaagaaagtc agaagttgac tttatcaaat ttaccgagcc
    15061 tgagcctatt tacagggaca gatcagggtc agtttgaagt gatgaagagt caagtgttga
    15121 aacagattgg gtatgattct gctgacctca actttgccta ctttgatatg aaagaagtag
    15181 tttacaagga tgtggaactg gagttggtca gccttccttt ctttgcggat gagaaaatcg
    15241 tgatattaga ttattttatg gatatcacga ctgctaagaa acgctttttg acagatgatg
    15301 agcttaagtc atttgaggaa taccttgaca atccttctcc aacaaccaag ttgataatct
    15361 ttgcagaagg aaagctggat agcaaaagac ggttagtcaa attacttaag cgtgatgcca
    15421 aggtcttcga tgcagtagaa gtaaaagaac aagaattgcg ccagtatttc caaaagtgga
    15481 gtcagaaaca aggtctgcag tttaccaatc attcttttga aaatctcctc atcaagtcgg
    15541 ggtttcaatt tagcgaaatc cagaaaaatc ttctcttttt acagtcctat aaggcgaatt
    15601 ctgttatcga ggaagaggat attgttaacg caattcccaa gaccttgcag gacaatattt
    15661 ttgatttaac tcagtttatt ctgactaaaa agatggatca ggcgcgcgat ttggtgagag
    15721 acttgacctt gcaaggggaa gatgaaatca aactgattgc agtcatgctg ggacaatttc
    15781 ggacttttac tcaggtgaag attttggcgg agtctggcca aacagaatcg cagattgcaa
    15841 gtagtttagg tagttatctg ggacgtaacc caaatcctta tcaaatcaag tttgcattaa
    15901 gagattcgag aggactttct ttgagctttc tgaagcaagc tatttcctat ttgattgaga
    15961 cagactatca gattaagaca ggtctttatg aaaaaggttt cctttttgaa aaggcactct
    16021 tacagattgc tagtcaggtc aattgacatt tgttgaaact acaacccacg tcaggattat
    16081 cttgtcgtgg gtttcttgct gatttgctta ttttattaga aagtatgtca gtgatttcct
    16141 tatctataag caaaaaactt gaaaaaagtg aatgtttgcg ttatcatttt catatagaaa
    16201 gaaaaagagg tattacagat ggctattatc ttaccagaac ttccatatgc atacgacgct
    16261 ttggaaccat acatcgatgc ggaaacaatg catttgcacc atgacaaaca ccatcaaact
    16321 tatgtcaaca atgccaatgc agctttagaa aaacaccctg aaatcggtga agaccttgaa
    16381 gccttgcttg ctgatgtaga atctatccca gctgatatcc gtcaagcact tatcaacaat
    16441 ggtggcggac acttgaacca cgctcttttc tgggaattga tgactcccga gaaaacagct
    16501 ccttcagcag aactgacagc agcaatcgat gcaacatttg gttcatttga agaattccaa
    16561 gcagccttca ctgcagcagc aacaactcgt tttggttcag gttgggcatg gttggttgtc
    16621 aacaaagaag ggaaacttga agtgacttca acagcaaacc aagacacacc aatctcagaa
    16681 ggtaaaaaac caatcttggg cttggacgtt tgggaacatg cttactacgt gaaataccgc
    16741 aacgtgcgtc ctgactatat caaagctttc ttttcagtaa tcaactggaa taaagtagat
    16801 gaattgtacg cagctgctaa ataatgatag ttggagggaa gaattgttct tctctttttt
    16861 aggttataag attctggtct gacaaaatcg tcagactttt ttcattttta tgagaaacgt
    16921 gctaactgct agaaattatg gtagaataaa gtattaagta atcgtggaaa aaggatatct
    16981 atgcgaaaag aaattgcacc tgaattatac aactataaca agtttcctgg tccggagttc
    17041 catttacacg gggacaaggt cgaaacggaa gggatagctt tttccttggt tgaaaatatc
    17101 aaggatgcct ttgatgtgac gacttttaat cagcgttttt cagaagtatt aaccaagttt
    17161 gattatatcg tgggggactg gagcaacgaa cagcttcgcc tacgaggttt ttacaaggat
    17221 gaccgaacag aagaaaaact tgaaaaaatc agtcgtttac aagactacct tttagagtat
    17281 tgtagttatg gttgtgccta ttttgtctta gaaaatgaag cccctaagcg agcatcattt
    17341 gacaagaaaa tgcgtaagac ggaagaagaa acaccttcta gaaaaggaaa gaaaccgact
    17401 caaactaaac gaaagtcgaa tgcagataag aaaaatagac gtcgtcagaa agaccaacat
    17461 tctcagaaag aggacaagga acaacgtcat tttgtcattc gtcagaagtg agtagagaat
    17521 aaggagaaga aatgaaaccc tcaattcata gtttggcaca tcaaaccatg caggaatggg
    17581 tattggagca gggagaaaag aaattccgtg cagatcaaat ctgggaatgg ctctaccgta
    17641 aacgtgtgca gtcatttgaa gaaatgacca acctttccaa ggatttgatt gctaagctca
    17701 atgaccagtt tgtggtcaat cccttgaaac aacgtattgt gcaagagtct gctgatggta
    17761 cagtcaaata tctctttgaa ttgcccgacg gtatgttaat tgagactgta ctcatgcgtc
    17821 aacactatgg tttatctgtc tgtgtgacca ctcaggtcag ctgtaatatc ggttgtacct
    17881 tctgtgcctc tggtttgatt aagaagcagc gtgacctcaa taacggtgaa atcgtagcgc
    17941 aaatcatgct ggttcagaaa tactttgctg atgaaggggt tcaggtcaat cttgccgtgt
    18001 ctcttcacgc acccaacaat gaacttcgtt caagtatcat gaaaattaac cgtgcctttc
    18061 cgattgaaaa actctttgct gctattgaat actacattga gacaacaaac cgtcgtgtaa
    18121 cctttgaata tatcatgctt aatgaagtca acgacggtgt agaacaagcc ttggaattgg
    18181 ctgaattgct caagaacatc aagaaattgt cttatgtaaa cttgattcct tataacccag
    18241 ttagtgagca tgaccaatat agccgtagtc ccaaagagcg cgtgttggct ttctatgata
    18301 cgcttaagaa aaaagggggc aactgtgtgg ttcgtcaaga gcatggtaca gatattgacg
    18361 cagcttgtgg acaattgcgc tttaacacaa tgaaacgtga ccgccagaag gcagtcgcag
    18421 cggtaaatcc ttaatgatga agaagactta taatcatatt ttggtctggg gagtcatttt
    18481 ctatagcatt tgcattgtct atttttgctt tactcctcaa gaacaatcta ccgtgggagt
    18541 gggaactcca gatattcagc ctcttggacg cctggttttt cttttgactc ctttcaattc
    18601 tctctggaaa ctgggcgaag tgagtgacat tggacaatta tgttggattt ttttacaaaa
    18661 tatcctcaat gtcttcttgt tttttcctct gattttccaa ctcctttatc tatttccaaa
    18721 tttgcggaaa acaaaaaagg tccttctttt tagttttctt gtgagtcttg gaatcgagtg
    18781 tacgcaatta atcttggact ttttctttga ttttaatcgc gtctttgaga ttgatgattt
    18841 gtggaccaac actttgggtg gctatctggc ttggctcctt tataaacgat tacataaaaa
    18901 caagataagg aattaaaatg agtattttag aagttaaaaa tctgagtcac ggttttggtg
    18961 accgtgcaat ttttgaagat gtgtccttcc gtctcctcaa gggagaacat atcggcctgg
    19021 tcggtgccaa tggtgaagga aaatcaacct ttatgagtat cgtgactggt aaaatgctgc
    19081 cagatgaagg aaaggttgag tggtccaaat atgtgacggc tggttacttg gatcagcact
    19141 ctgtccttgc tgaaagacag tcggtgcgtg atgttctccg tacggctttt gatgagcttt
    19201 tcaaagctga agctcgtatc aatgacctct atatggaaat ggctgaagac ggtgcggatg
    19261 ttgatgctct catggaagaa gtgggagaac ttcaagaccg tctggagagt cgtgatttct
    19321 ataccttgga tgctaagatt gacgaagtag cgcgtgctct tggtattatg gactttggca
    19381 tggatacgga tgtaacttct ttgtcaggtg ggcaaagaac caaggtgctt ttggcaaaac
    19441 ttctccttga aaagcctgat attttgctgt tggacgagcc gaccaactac ttggatgctg
    19501 agcatattga ttggctcaag cgctatctcc aaaactatga gaatgccttt gtcctcattt
    19561 cgcacgatat tccattcctc aatgacgtta ttaatattgt ctatcatgtg gaaaatcaac
    19621 agctgacgcg ttactctggt gactactacc agttccaaga agtttatgct atgaagaaat
    19681 ctcagctaga ggcagcctac gaacgccagc agaaagagat tgcagacctc aaggactttg
    19741 tggctcgtaa taaagcccgt gttgcaactc gtaatatggc tatgtctcgt caaaagaaat
    19801 tggataagat ggatattatc gaactccaaa gtgagaaacc aaaaccatcc tttgatttca
    19861 aaccagctcg tacaccaggg cgctttatct tccaagccaa gaacttgcaa attggttacg
    19921 accgtcctct tactaagcct ttaaatctta ccttcgaacg caatcaaaag gttgcgatta
    19981 ttggtgctaa tggtattgga aaaacaactc tcttgaagag tctcttaggc attatctcgc
    20041 caatcgctgg ggaagtggag cgtggagatt atttagaact tggttatttt gagcaggaag
    20101 tagaaggcgg taatcgccaa actcctcttg aagctgtctg gaatgccttt cctgccctta
    20161 atcaagcaga agtccgtgca gcccttgccc gttgtggttt gacaaccaaa catattgaaa
    20221 gccagattca agtattatca gggggagagc aagccaaggt tcgtttctgt ctcttgatga
    20281 atcgtgaaaa caacgtttta gtgctggacg agccgaccaa ccatttggat gtggatgcaa
    20341 aggatgagct caaacgcgct ctcaaagaat ataggggatc tatccttatg gtctgccacg
    20401 agccagactt ttatgaaggc tggatagacc aaatatggga ttttaataat ttaacttaag
    20461 aatgagagta aaagaaatac agtaccgaat gtggatactg tatttttttg gtttttaaga
    20521 tttaaaatcg tagtctttca ccacttcgat gctgtcgatt gtgatagcag tagttggctt
    20581 gtctttttca tctttttcgg ccttagcaat cttatccaca acatccatac cgtcaatcac
    20641 ttgaccaaag actgggtgtt tgccatctag actagggttt ccaccttctt tgtaggcttc
    20701 gataattttc tgtggatact tgcttgtagg gagtttagaa gaggtatctg tagagttttg
    20761 gttgatgaag aactggctgc cattggtgtt tggttgacca gtattagcca tagcaagagc
    20821 accacggatg ttatacaaat aaggagtaat ctcgttcttg aaaccagttc ctttgtcttt
    20881 agtcttatcc ttgtcatgcc agatggactg accacctgta ccgtcccctt ttggatctcc
    20941 agtttggacc ataaagccat cgatgacacg gtggaaggta ataccgttat agtagccttc
    21001 tttggcgtga gtgaggaaat tttcaaccgc tagaggagcg agtttaggga agagtttaat
    21061 gcgaatatca ccttggcttg tgtggagaat cacttcggct tcatcttcag caacttcctt
    21121 agatagttgt gggaaattgg cgttttcgtt tgttaaagca tcgtttaact ccttggcaga
    21181 ttgggcagct actttggaac tttcttcagc agcaagactg gaatcaacat aatcatcacc
    21241 acgcagactg cgttggacgc tgctacaccc agctagggct acagtagaca gtaaaagaag
    21301 ggttgctagt tttttcattc tgatcctctc aaaaattcat ttataaccat tgtaccctaa
    21361 aaggaagtgg atttcaaata ttagtagttt acagaactgg cttagataat agttgggatt
    21421 tcatcatcat cttgaaaatc ttcaacttct tccccagtaa atggtttctg aaaagaactt
    21481 agtataaaac gggcaattgg ttcaacaatt aggaaattta gaggaagagc tgcaataaag
    21541 tttacgaacc aggcgcttag ataggtattg ggtgtgatag caatgccatt gtaaaaaaag
    21601 tccatagaag gacatgaaag tgaccagcta attcttcttc tgaaaaatgc tcatgaaggg
    21661 cttgatccag gtcgcaagat ttcattttag aaagtccagt aactcccttc ttcttcagga
    21721 tattcttttt catggtagta tttagatggt caagtgagtc aaaagaagtt tcgacaagag
    21781 catacccttt ttctaccaga gtttttagat gtttaggggc atcaatacca taagtgtact
    21841 caaagtattt tggaaaccag atcttaagaa agctcgtaaa gctagccagt tctctaaacg
    21901 ctaagaacta gctacaacac agcatttaca acacttcatg gttcacacca tggggtgttt
    21961 tttaatgatt ttaagcaaaa ttcacactta attgaaagtt atttaaatta gatgaaaatt
    22021 tattttttga aaaagtggga aaacattgat tctaagcgaa ttttgaatca tattcgaatg
    22081 ggagttttta aaatcatgtt tcagtggtaa aagtaccaaa ctggattctc catagtaaaa
    22141 tgatatctcc tgctagtgaa tcatctacta ataagtccat atttcgcttg ggaacgggct
    22201 tgggacttag aagccaagta tccaactctc gttcaggcga tatgaagggt ttgacttggt
    22261 gttctttata aaatttttct aaaatagtat tcacggatag catctttcaa acatttattt
    22321 cctattctaa caaatttcta aatactttaa gaaatttata gtctgtcaag tttttttctt
    22381 gacacatgtc agaaatctgc tataatagaa catgtgctaa atagctcagc tatttcaccg
    22441 aaataaaaaa ttaaagaaat gagactttaa aaatggcagt taaaatccgt ttgactcgta
    22501 tgggttctaa gaaaaaacct ttctaccgta tcaacgtagc agattcacgt tcaccacgtg
    22561 acggacgttt catcgaaaca gttggaactt acaacccact tgttgctgaa aaccaagtaa
    22621 ctttgaaaga agaccgcgtt cttgcatggt tggctaatgg agctcaacct tcagacacag
    22681 tacgtaacat cctttcaaaa gaaggcgtat tgaaaaaatt ccacgattct aaattttcaa
    22741 aataagttta aagtaggttg acagatggat acgattgaaa atctcattat tgcgattgtg
    22801 aaacccttaa tttcacaacc agatgcctta actatcaaga ttgaggatac accagaattt
    22861 ttggaatatc atttgaatct tgatcaaagc gatgtgggtc gtgtaatcgg tcgtaagggt
    22921 cgcactattt ctgcgataag aacgattgtc tactctgtcc caactgaata caaaaaagta
    22981 agaatcgtta ttgacgaaaa ataagaaggg cgggacggat gtctcgcttt tttgtgtgga
    23041 atagaag
//
LOCUS       DBEJAQ010000033        22810 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.033, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000033 DBEJAQ010000000
VERSION     DBEJAQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.033"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            467..1588
                     /gene="mnmA"
                     /locus_tag="ACL297_001400"
     CDS             467..1588
                     /gene="mnmA"
                     /locus_tag="ACL297_001400"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGR0071656.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1729..2184
                     /locus_tag="ACL297_001401"
     CDS             1729..2184
                     /locus_tag="ACL297_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGR0071657.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQYGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2194..4107
                     /gene="mnmG"
                     /locus_tag="ACL297_001402"
     CDS             2194..4107
                     /gene="mnmG"
                     /locus_tag="ACL297_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGR0071658.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4460..6139)
                     /locus_tag="ACL297_001403"
     CDS             complement(4460..6139)
                     /locus_tag="ACL297_001403"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGR0071659.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6141..6374)
                     /locus_tag="ACL297_001404"
     CDS             complement(6141..6374)
                     /locus_tag="ACL297_001404"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HGR0071660.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6654..6869
                     /locus_tag="ACL297_001405"
     CDS             6654..6869
                     /locus_tag="ACL297_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071661.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(7192..7344)
                     /gene="cibB"
                     /locus_tag="ACL297_001406"
     CDS             complement(7192..7344)
                     /gene="cibB"
                     /locus_tag="ACL297_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HGR0071662.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7346..7531)
                     /gene="cibA"
                     /locus_tag="ACL297_001407"
     CDS             complement(7346..7531)
                     /gene="cibA"
                     /locus_tag="ACL297_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HGR0071663.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7709..8392
                     /gene="tsaB"
                     /locus_tag="ACL297_001408"
     CDS             7709..8392
                     /gene="tsaB"
                     /locus_tag="ACL297_001408"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGR0071664.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8389..8826
                     /gene="rimI"
                     /locus_tag="ACL297_001409"
     CDS             8389..8826
                     /gene="rimI"
                     /locus_tag="ACL297_001409"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGR0071665.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYVLAYDGAEVIGFLAVQENIFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8816..9826
                     /gene="tsaD"
                     /locus_tag="ACL297_001410"
     CDS             8816..9826
                     /gene="tsaD"
                     /locus_tag="ACL297_001410"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGR0071666.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9866..11172)
                     /locus_tag="ACL297_001411"
                     /pseudo
     CDS             complement(9866..11172)
                     /locus_tag="ACL297_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11508..12199)
                     /locus_tag="ACL297_001412"
                     /pseudo
     CDS             complement(11508..12199)
                     /locus_tag="ACL297_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12442..12996
                     /locus_tag="ACL297_001413"
     CDS             12442..12996
                     /locus_tag="ACL297_001413"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGR0071667.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIQLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWT
                     YSMGDAFICVSETMKKRLESIIKK"
     gene            13124..13648
                     /locus_tag="ACL297_001414"
     CDS             13124..13648
                     /locus_tag="ACL297_001414"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGR0071668.1"
                     /translation="MDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICH
                     LKCRDNFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLS
                     EIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVET
                     MRVYDELLEKSSHG"
     gene            13641..14564
                     /locus_tag="ACL297_001415"
     CDS             13641..14564
                     /locus_tag="ACL297_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGR0071669.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMSDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLFFEIYVIISFWFFLLYI"
     gene            14605..16221
                     /locus_tag="ACL297_001416"
     CDS             14605..16221
                     /locus_tag="ACL297_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0071670.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            17010..17759
                     /locus_tag="ACL297_001417"
     CDS             17010..17759
                     /locus_tag="ACL297_001417"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGR0071671.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            17772..18944
                     /locus_tag="ACL297_001418"
     CDS             17772..18944
                     /locus_tag="ACL297_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGR0071672.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPFGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNLSFGFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            19340..20203
                     /locus_tag="ACL297_001419"
     CDS             19340..20203
                     /locus_tag="ACL297_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGR0071673.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            20489..20689
                     /locus_tag="ACL297_001420"
     CDS             20489..20689
                     /locus_tag="ACL297_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HGR0071674.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            20756..21421
                     /locus_tag="ACL297_001421"
     CDS             20756..21421
                     /locus_tag="ACL297_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGR0071675.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            21493..22719
                     /locus_tag="ACL297_001422"
     CDS             21493..22719
                     /locus_tag="ACL297_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGR0071676.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLF
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         6968 a         3885 c         4837 g         7120 t
ORIGIN      
        1 aggtgccctt gcagtcaaca caactgtaga cggatatact gtaaatgaaa acggtgaatg
       61 ggtttaagcc gattaaatta aatcatgtta agaacatttg acattttaat tttgaaacaa
      121 agataaggtt cgattgaata gatttatgtt cgtattcttt aggtacctgt cttatgattt
      181 caggaaatgt cattaaaaaa cgactccttt tctctaactt gaaaaataga ttagagaaaa
      241 tgggttgttt tatttattat agttatttga atgaagataa gaagaaggta tactcacgtc
      301 attcacataa tctgtatatt gactataagt tttaaaaaac aatttttaag ctcttccttg
      361 tcttctctaa ccaagcgtgt tataatgaat actgctcaag cgaccttcaa tcgtgaagca
      421 cacacgacct tcaatcgtga ataaacgaat agatgggaga cttaccatga gtgataactc
      481 taaaacacgt gttgtcgtgg ggatgagtgg tggtgttgat tcgtcggtga cggctctttt
      541 gctcaaggag cagggctacg atgtgatcgg tatcttcatg aagaactggg atgacacaga
      601 tgaaaacggc gtctgtacgg cgaccgaaga ttacaaggat gtggttgcgg tggcagacca
      661 gattggcatt ccctactact ctgtcaattt tgaaaaagag tactgggacc gcgtttttga
      721 gtatttccta gcggaatacc gtgcagggcg cacgccaaat ccggacgtta tgtgcaacaa
      781 ggaaatcaag ttcaaggcct ttttggacta tgccataacc ttgggggcag actatgtagc
      841 gactgggcat tatgctcgag tggcgcgtga tgaggacggc actgttcaca tgcttcgtgg
      901 cgtggacaat ggcaaggacc agacctattt cctcagccaa ctttcgcaag aacaacttca
      961 aaaaaccatg ttcccactag gacatttgga aaagcctgaa gttcgaaaac tagcagaaga
     1021 agcaggcctt tcgactgcta agaagaaaga ctcgacaggg atttgcttta tcggagaaaa
     1081 gaactttaaa aacttcctca gcaactacct gccagctcag cctggtcgca tgatgactgt
     1141 ggatggtcgc gatatgggcg agcatgcagg tcttatgtac tatacaatcg gtcagcgtgg
     1201 cggactcggt atcggtgggc aacacggcgg tgacaatgcc ccttggttcg ttgtcggaaa
     1261 agatctaagc aagaatattc tctatgtagg acaaggattc taccatgatt cgctcatgtc
     1321 aactagccta gaagccagtc aagtccactt tactcgtgaa atgccagaag agtttacgct
     1381 agaatgtacg gctaaattcc gttaccgtca gcctgactct aaggtgaccg tccatgtcaa
     1441 aggagacaag gcagaggtca tctttgcgga accacaacgc gcgattacac caggacaggc
     1501 agttgtcttt tacgatggcg aagagtgtct aggtggtggt ttgattgaca atgcttaccg
     1561 cgatggacaa gtttgtcagt acatttagat tgacaaattt tctcaatttg ctacaataat
     1621 aacagcaata gaaatgatgg tcaaagctca tggatgttgc aggctttttt gtcctgcact
     1681 tctttgtagt tttgactgtt tttgtgtcgt ttaagggaaa ggacaagaat gactcaacaa
     1741 gactttcgga caaaagtaga caatacggtt tttggtgttc gggcgacagc cttgattgtc
     1801 caaaatcaca agcttctagt taccaaagac aagggcaagt attacactat tggaggtgcg
     1861 attcaagtca atgaaagcac ggaagacgcg gtagtccgtg aagtgaagga agaactgggt
     1921 gttaaagctc aagctgggca gctagctttt gtggttgaaa atcgttttga acaatacggt
     1981 gtttcctatc acaatatcga gtttcattat ctggtggatt tgcttgaaga tgccccattg
     2041 accatgcagg aagatgagaa aaggcagccc tgtgagtgga ttgacttgga taagctccag
     2101 aatatccagc tagttccagc ctttttaaaa acagccctac cagattggga aggccaacta
     2161 agacacattc atcttgagga ataggagaga aacatgactt atcattttac tgaagaatac
     2221 gatattattg taattggtgc gggacacgct ggggttgagg cttccttggc cgctagccgt
     2281 atgggctgta aggtcctgct tgcgaccatc aatattgaaa tgctggcttt catgccttgt
     2341 aatccctcta tcggtggttc tgccaagggg attgtcgtgc gtgaagtcga tgccctcggt
     2401 ggcgagatgg ccaaaaccat tgacaagact tacatccaga tgaagatgct aaacacaggg
     2461 aaggggccag ctgtccgtgc ccttcgtgcg caggctgaca aggaacttta ctctaaggag
     2521 atgcgcaaga cggttgaaaa ccaagaaaat ctgacccttc gtcaaaccat gattgatgag
     2581 attttggtgg aagatggcaa ggttgtcggt gtgcgtacag ccacccatca agaatatgct
     2641 gctaaggctg ttattgtgac gacagggact gctctccgtg gggaaattat catcggagac
     2701 ctcaagtact catcaggtcc taaccacagc ttggcttcta ttaacctagc tgacaatctc
     2761 aaggaactgg gtctcgaaat cggtcgtttc aagacaggaa cccctccacg cgtcaaggcc
     2821 tcttctatca actacgacgt gacagaaatc caaccaggag atgaagcgcc taatcacttc
     2881 tcatacactt cacgtgatga ggattatgtc aaggaccaag tgccatgctg gttgacctat
     2941 accaatggta ccagtcatga gattattcaa aacaacctcc accgtgcgcc tatgtttaca
     3001 ggtgtggtca agggagtggg gcctcgttac tgtccgtcga ttgaagacaa gattgtgcgc
     3061 tttgcggaca aggaacgtca ccaactcttc cttgagccag aagggcgcaa tactgaggaa
     3121 gtctatgttc aaggactttc aaccagtctg cctgaggatg tccagcgtga cttggttcat
     3181 tccatcaaag gtttggaaaa tgcagagatg atgcggacag gttatgctat tgagtatgat
     3241 atggtcttgc ctcatcagtt gcgtgcgact ttggaaacca agaaaatctc aggtctcttc
     3301 actgctggtc agacaaatgg aacatcaggt tacgaagaag ctgctggcca agggattatc
     3361 gcgggtatca atgcggctct gaaaatccaa ggcaagcctg aattgatttt gaagcgcagt
     3421 gatggttata tcggggtgat gatcgacgac ttggtgacca agggaaccat tgaaccctac
     3481 cgtctcttga ccagtcgtgc tgaataccgt ctcattcttc gtcatgacaa tgctgatatg
     3541 cgcttgactg agatgggacg cgagattggc cttgtggacg atgaacgctg ggctcgtttt
     3601 gaaatcaaga aaaatcaatt tgataatgag atgaagcgcc tagacagtat caaacttaag
     3661 ccagtcaagg aaaccaatgc caaggttgag gagatgggct tcaaaccctt gaccgatgca
     3721 gtgacagcca aggaattcct tcgccgtcca gaagtttctt accaagatgt ggtggccttc
     3781 atcggaccag ctgcagaaga cttggatgac aagattatcg aattgattga aacagaaatc
     3841 aagtatgaag gctatatttc caaagccatg gaccaggttg ccaagatgaa acgcatggaa
     3901 gaaaaacgca ttccggccaa tatcgactgg gatgacattg attctatcgc aaccgaagcc
     3961 cgtcagaagt tcaaactcat caatccagaa accatcggcc aagccagccg tatttcggga
     4021 gtaaacccag cagatatttc tattttgatg gtgtatctgg aaggtaaaaa tcgtagtatt
     4081 tctaaaactc ttcaaaaatc aaaatgatac gtcgtcggct tcttacgaat gagttcaaag
     4141 cttggctttg attcatctcc agcctcccat agtttcccga actatgggag ctaactcaag
     4201 ttatgctttc gcttagcctt ctagtctgat ttcgattttt attgatactc ttcgaaaatc
     4261 tcttcaaacc gcgtcaacgt cgccttgccg taggtatggc tactgacttc gtcagttcta
     4321 tctacaacct caaagcagtg ctttgagcaa cctacggcta gcttcctagt ttgctctttg
     4381 attttcattg agtatgagtt ttaatctcaa aagaaagcat agagaaaaac agctccgagg
     4441 acggggctgt tttcttgctt tattcttcat ctggtgtgag gatcatcggg atgatgatcg
     4501 gttcacgttc ggtattttca tagaggaagg ggcgaatagc gttgacaatg gcaccattga
     4561 cagattgcac gctggcatcc ttatttttca gtgcgatacg aatggcattg aagaggatac
     4621 gctggctttg gcgaatcaag tcgccagact ctctcatgta gacaaagcct cggctgagga
     4681 tgtctggacc agacagaatc atctgcgatt tgaagtcaac agttgcgact gccagaacga
     4741 caccgtcttc agatagatcg cgacgatctt tgaggacagc tgcgccaatt tcaccgatac
     4801 gatttccatc gacatagata tcttgggcgt tgaaatgacc tgcgatacga gctgagtcag
     4861 cagtaagggc aagcacatcg ccattgctca tgataaagat attgtccttc tcaacaccag
     4921 tatccactgc tagtccagcg tggacttttt gcatgcggta ttcaccgtgg acaggcatga
     4981 agtattttgg cttaatcaag cagagcatga gtttttgctc ttgctgacca ccgtgtccag
     5041 atgtatggat attgttcact ttaccgtgga taacttcgac accagcttca gaaatgatgt
     5101 taatcagctt gttgacgcta gtagtgtttc cagggatggg actagaagag aagataacgg
     5161 tatcacctgg ttgtaattgt acttgacggt gggttccgtt ggcgatacga gagagagctg
     5221 ccataggctc accctgacta cctgtacaga ggataagaac ttctcctgca ggataatctt
     5281 tgatttcatt tggctcgata aaggttccct taggagcttt gatgtagcca agatcgattc
     5341 cgttgacaat ggccttttcc atagaacgac caaagaccgc aatcttgcgt ccagtcttaa
     5401 cagcagcttc tgttgcctgc tggagacgga agatatttga ggcaaaggat gcaaagatga
     5461 tacgtccttc aataccttgg ataatcttca taatggactg accaacgact ttttcagagt
     5521 tggtaaaggt tggtacttcc gcatttgtcg agtcagacag gagacagagc acgccttctt
     5581 caccaagcgc agccatacga tgcaagtccg caggttctcc aactggagta aagtcgaact
     5641 taaagtcacc cgtacagacg attttccctt gaggagtatg aatgacaatc cccaaaggct
     5701 ctggaataga gtgagtcgtt ctaaagaaag ttgccttgag atttttaaag gtcaactcgg
     5761 tgttgtggtt gatttcgtaa agtttggcgt tgcgcaagag gccgtgttct tcgagtttcc
     5821 cacggatcaa agccaaggca agcggtccag cgtagatagg gacatttgct tgcttgagta
     5881 ggaacggaat cccaccaatg tggtcctcgt gtccgtgtgt gattaaaaca gccttgacgc
     5941 ggtcgatatt gtccacgatg taagagtagt caggaatgac atagtcgata ccaagcaagt
     6001 catcttctgg gaatttaatc ccagcatcga cgatgataat ctcgtcttgg tattcaattc
     6061 cgtaagtgtt tttcccgatt tctcccagac caccgatggc aaaaacgccg acttcttcag
     6121 gtttaagagt ataggccata ttagaactcc gtgatttcga aggcgccagt ttctttttcg
     6181 taatcgagca atttgtcaga caagagttcg atatattcga tattgtactc tgggcgattt
     6241 tcttcgacaa gttggcgagc agtgatgcga ccctcaagtt ctgagctggc atcgatgtct
     6301 aggtaaagtg cgcgtgttgt ttcacggcgt gggctacgtt cttttgtttc ttgataaaaa
     6361 actttgtaaa tcatatagtt cctttctatt tacaggtcag cttattccaa acttttagca
     6421 gatatttgct tgatttcatt ccattttgtg tctagattga ccttgattcg ttacaattat
     6481 ttcaattata ccacaaaatg aaaaattagt aaaagacgga aacgagaaag ttaggggacg
     6541 atttctttgg taagcgtttg caagaataaa gaaaatgtat tatgttgata acggaagtga
     6601 aatttattgc ttgtcataat tccatataga ttttaagtca aggaggttgc cctatgtata
     6661 acttatttgc ttttgctatc ggagctcgct tggtagcttt tattgttttt gttgcttgcg
     6721 tttatgcggg gaatcttcta tttgccaaac tggaacgacg acaaaccaag ttcttgtgga
     6781 aaatgacctt tgtgactctc tactcgcttt ttctcctcct cgtgacctat gtcgtttggt
     6841 ttgtatttta ctttgaatta aatgcttaga tgccctgctt atgctggggt tttttgtttg
     6901 gggatgtaga gatagtagat ttacgacacg aaaattccac cccatctatc aagatgaggt
     6961 ggtctttgca tttttctaac atgacatgac aatggaattt tcaaagctta ttatttttat
     7021 gggagtcatt gttctgatca aaaatagagt tgaagcataa aaataaaaca atacaaacag
     7081 ataaatcgaa tggtaaaggg atggtagtaa tgttcaaatc attgagaatg ctgagcaaac
     7141 tgtaagagat gaagacaata aaatttatcc ttctaaaaat gtgtttctta tctagcatga
     7201 cttaccaaac tttttacgaa ggtcttttcc aatggtataa cctgccataa ataaagcgat
     7261 accaacaccg acaccaaagc cacccttgat ttcttgcaat tccttattag aaatttcacg
     7321 ataattgttc aaatctttca tcatattaat ctcctcctaa aaaataacca gctgccaaac
     7381 cagaacctat aagtgctgtt ccacctgcaa tagccttcca agatactgct actccaaaga
     7441 taaccaaggg agcgagaccg ccatcaatat ctgataattc attttcagat aaggataaaa
     7501 attgattgtc aagaatgtca aaatttgtca ttgaactttc ctcctttttt cttgttcact
     7561 tttatattcg gaaaagaaaa ggaaaagtga gcattttttt aaattatttt tagttaagtg
     7621 agggcactct tgtctggggg caaagaaaat tcccatgtgc cagcttttgt agtataatag
     7681 taagcagaca aaaagatagg aatgtgttat gaaagtatta gcttttgata cgtccagcaa
     7741 ggctctttct ctggctattt tagaggataa gcaggttctt gccgagacga cgattaatat
     7801 taagaaaaat cacagtatta ctcttatgcc tgccatcgat tttttgatgg caagtttgga
     7861 ttggacaccc aaggatttgg accgaatcgt ggtagctgaa gggccgggta gctatacagg
     7921 cttgcgaatt gcggtagcaa ctgctaagac cttagcttac accctgaaca tcgagttggt
     7981 tggtatgtcg agtctcttgg ctctggtgcc ccaccaacaa gaaggtttgt ttgtcccctt
     8041 gatggatgcg cgtcgcaata atgtttatgc aggattttat gaaaatgcca aacctgtcat
     8101 ggcagaagcg cacctatctt ttgaagaggt gctagaaaaa gtcaagggta ctagtcaggt
     8161 aacctttgtc ggagaagttg gcccctttgt tgagcagatt caaaaacact tgccaaggac
     8221 tgattacaaa gaaacattgc ccaatgcagc caatcttgct cttttagctt gggacaagga
     8281 agcagactcc ttgcatgatt ttgtgccgaa ttacctcaaa cgagtcgagg ctgaggaaaa
     8341 ctggctcaag aaccataccg agtctggtga gtcctacatt aaacgcctat gatagaaatc
     8401 aagcgaattc aacaacagcc tgacctagct caagccatct acgctgttat ggcagctgtt
     8461 tacctagtca gtccttggac tctggagcaa atccaagcag acctgtccca agaccagact
     8521 tggtatgttc tggcttatga tggggcagaa gtgattggtt ttctagctgt tcaggaaaat
     8581 atctttgagg cagaagtctt gcaaatcgct gttaaaggag cttatcaggg tcagggaatt
     8641 gcgtcagcct tgtttgctca attgccgaca gataaggaga ttttcctcga agtcagaaag
     8701 tcaaatcaac gagcgcaagc attttacaag aaagaaaaaa tggcggtcat cgctgagcga
     8761 aaggcctact accatgaccc agtcgaggac gccattatca tgaagagaga aatagatgaa
     8821 ggatagatat attttagcat ttgagacatc ctgtgatgag accagtgtcg ccgtcttgaa
     8881 aaacgacgat gagctcttgt ccaatgtcat tgctagtcaa attgagagtc acaaacgttt
     8941 tggtggcgta gtgcccgaag tagccagtcg tcaccatgtc gaggtcatta cagcctgtat
     9001 cgaggaggca ttggcagaag cagggattac cgaagaggac gtgacagctg ttgcggttac
     9061 ctacggacca ggcttggtcg gagccttgct agttggtttg tcagctgcca aggcctttgc
     9121 ttgggctcac ggacttccac tgattcctgt taatcacatg gctgggcacc tcatggcagc
     9181 tcagagtgtg gagcctttgg agtttccctt gctagccctc ttggttagcg gtgggcacac
     9241 agagttggtc tatgtttcgg aggcaggaga ttacaagatt gttggggaga cacgagacga
     9301 tgcagttggg gaggcttatg acaaggtcgg tcgtgtcatg ggcttgacct atcctgcagg
     9361 tcgtgagatt gacgagctgg ctcatcaggg gcaggatatt tatgatttcc cccgtgccat
     9421 gattaaggaa gataatctgg agttctcctt ctcaggtttg aaatctgcct ttatcaatct
     9481 tcatcacaat gccgagcaaa agggagaaag cctgtctaca gaagatttgt gtgcttcctt
     9541 ccaagcagca gttatggaca ttctcatggc aaaaaccaag aaggctttgg agaaatatcc
     9601 tgttaaaacc ctagttgtgg caggtggtgt ggcagccaat aaaggtctca gagaacgcct
     9661 agcagccgaa atcacagatg tcaaggttat catcccccct ctgcgactct gcggagacaa
     9721 tgcaggtatg attgcctatg ccagcgtcag cgagtggaac aaagaaaact tcgcaggctg
     9781 ggacctcaat gccaaaccaa gtcttgcctt tgataccatg gaataaagag tggctctttg
     9841 tcaagtgtag tgggtaggcg aaaagctaca atctggagag tacgaaattc gtactctctt
     9901 ttttgatgtt caaagtcatc aaaatccgtt ttttaaaatt gttaaagttc cggaaaccaa
     9961 aggctttgcg cttgataacc ttaatcaaat tattggtcgc ttcaagttta gcgttggaat
    10021 aaggcagctt aagtgcgttg ataatcttat ctctatccct taaaaaagtc caaaagaccg
    10081 tttgaaaaag cggatgaacc gttggcagtt cttgctcaat caatccaaag aaatggtcgg
    10141 cattcttctc ttgaaaatga aagagcagga gttgatagag ttcgtagtga acctgtaatc
    10201 cctcagagta agaaagcagc ttatctcgta cttcccgatg ggttaagtgc attcgaaacg
    10261 tgggctgata gaatcgtttc tgacagagct tgaagctatc ctggtgtagt aaatcagtcc
    10321 agtggactga tttagcccga gcctagaaac gaaaaagcga gggttccaaa agcgcttgag
    10381 cgcccgatac tccaaggatt ttcggtcaaa ttggttcatg atttggattc gtactcggtt
    10441 catagctcgg ctcagatgtt ggacaatgtg gaagcggtca agaacaatct tcgcctttgg
    10501 aaagagttgc ttagcgagcc gataataagg gctgtacatg tccatggtga tgacctcgac
    10561 cagctcccga acctctctct gatagcgttg gaaatggttt cgaatcaccg catgagttcg
    10621 cccgtctaaa attgcgagga tggatttgga ctcaaaatct tgggcaatga agctcatttt
    10681 gctctttttg aagctatact catcccaact cataactttt ggcaacttgg tccaatcggt
    10741 ttcaaactta aattccctca gttttcggat gacggtggaa gttgagaccg ccaatctgtg
    10801 ggcaatctca gtcatagact gcttttcaag gaggagctga gcgatttttt gtctcaccat
    10861 gttggaaatc tggcaatttt tcttgacaat ggatgtttga gaaacgacca ccttaaggca
    10921 attcttgcac tgaaagcggc gctttttgag atgtagtacc gtgggtaaac cctgacagtc
    10981 gagaagcgga attttagagg ctttctggaa gtcgtatttg atcatcttcc cttgacaatg
    11041 aggacaagga ggagcggggg aatccaactt tgcctgaacg actagatggg tttggtattt
    11101 cagaacagac aagattttga tatttttgtc tttcaatccg agcaaatctg tggtattctt
    11161 aagttgttcc atatgaaccc tttctaatga gttggttaga cgcttttcat tataggtcat
    11221 atggggcttt ttttctacaa gaaacgaccc tataattcct ggggtgggat tacccactac
    11281 agaaattata gagccaaaac attccaaagt cttgtctgac tctggaatgt tttctgttca
    11341 cagttgtttt gtaactgcct aataagtaac cgcgctataa cgaggtatct accgagattg
    11401 tcaattctcc agtatactgt tataaaaaac aaaactggag gattttttta tgtcccccca
    11461 tcgtcccatc gactgttccg tagctgccca ataacggagt atagtcggct gaaacaagaa
    11521 taggacaaaa gtgcctcaag aaaagcatcg taatttgaca atattattct gagatgtttt
    11581 tgatgtaagc ccatattttc tcaatgggat tatactcggg tgaataggta ggaagtggta
    11641 acagtctatg ccctgctcct tacacatgtt cattctgtga aaccttgcat tgtccataat
    11701 gataagggat ggtgtatcta aagtaggtag taagaatttt tggaagcaag cttcgaaaaa
    11761 gtcactcgtc atcgtatctt tataggtcat cggagccata gtatattgaa acaaaaaagg
    11821 gcattagtac ttctataaca gtatcgcaat ctctggtgtg cgttgtctcg atcttaattc
    11881 aaataattat atcttcttcc agagagacta actgcctgct caaggctcga tcatattcga
    11941 gataaaaata ggtctcaacc cctgtctcat aaatataaat aggagtcagg tagcttaagt
    12001 gattcaattc tttaaggaac cgatttactt tttctgggtc ttgttcgcag taggtacagc
    12061 tcttttttag actatatccc atagccttta gagcgtaatg aatagttgtt ggaggacagt
    12121 tgaattcagc agctatttca gtcaaataag catcgggatg agtttcaata tagatcttta
    12181 atttatctct atcaactttt tcaattaata gtggaagttt tcttaaagtt agtgtgttga
    12241 aacgttttta tacagtgcta aaatttgaag tgtagtgcag atatatttgt aatagtatta
    12301 tagcaattct ttggagaagg tcgagacaag agaaaatagg tatctttgag atataagcat
    12361 gatttactat aaaattattg agttattcat aaaaatttct ctatcaaata tcataatgcg
    12421 tcgtttaagg agatggttac aatgcaatat agttgtggaa aaataaatat taatatccct
    12481 gatggatatg gagacattaa ggatatagtg ttttcggctc atattatagt taggtataat
    12541 aatggacatt gtggaggtat agatccgcat ataattggac tttgtaaaaa gcagatcaga
    12601 aggatgtctc tatatcctat tttgataatc gtatctagag attcaaaggt tattgatgat
    12661 tataaaaatt tagatattgc ctatgttgat tgtactcaat gttcaaataa ttttgaaact
    12721 gccttacacg ttaaaaatat tttaaaatta ttaaaaatcc aacttataca ctgtcatggc
    12781 tactcaacta attatttttt atatatgtta aaaaaacttg acaagaatgg atttggaaag
    12841 gttaagacag tgataacatg ccatggatgg gttgaatata acttaaaaaa gaaattcttg
    12901 acttattttg atttttggac atattctatg ggagatgctt ttatttgtgt atctgaaact
    12961 atgaaaaaaa gattggagag tataataaaa aaataagaag atagttgtaa taaataacgg
    13021 aattaatgta tcaaatagcg atttagatgt agtaggtgtt caagacttta aaaaagaatt
    13081 ttgtattcca aataacaaaa aaatcatttg ttatgttgga aggttggatc cagaaaaaag
    13141 gccggataga ttccttgaat ttgcagaaaa attattttta gtcagagaag atgtgatttt
    13201 tattatggct ggaaatggta gtatgtgggc tgctttgaaa gaaaaaattt gccatttgaa
    13261 atgtagagat aatttcaggt tacttggtga aatctatcca gctactatag tttaccaaat
    13321 atcggattta ttgtatattc cttctgatac agaaggaata cctatgtgtg ttttagagtc
    13381 aatgtcacaa ggaactccgg ttttagctag taatgttggc gggttaagtg aaattattga
    13441 acatagggtt gatggatttt tatttgagaa ggaagatgtt gagggagtgt gtgcttgtgc
    13501 taatttttta ctcaatgatt ctgagtattt gaaatatata ggtgagaata gtaaatcaaa
    13561 aataagaaaa catttttctg tgcaaaaaat gtttgtagaa accatgagag tatatgatga
    13621 attattagag aagagtagtc atggatagga atatagatca agaattagta agtattataa
    13681 taccaacaca taataggtat gaatctctga ttagagctgt gaaaagttgt ttacatcaaa
    13741 gttataaaaa tatcgaagtt ataattattg atgataatta tagtaatgta aatttaagaa
    13801 ataaaattat ccatcaattt ggctatacca atcatagaat taagttaatt ttaagtaatg
    13861 aagatttagg tgcaactaat gccagaaaca taggtatcaa aaattctaga ggtaagtata
    13921 tatcattttt agacgatgat gatgaatata tgtcagatcg aattttaaag ttgatggctt
    13981 gttttaaaaa gagtagaatg aagaatttag ctttagttta tagttatgga ataataattt
    14041 atccaaatgg tacacgagaa gaggagaaga ccgattttgt tggaaatccc ttgtttgttc
    14101 aaatggttca caatatagca ggtacgtcat tttggttgtg taaaaaagag gtgctagaat
    14161 taattaatgg ttttgagaaa atagattcac atcaggacgg tgttgtttta ttaaaactac
    14221 ttgctcaagg ataccaaatt gatatagtgc gagaattctt ggtgaattac tacgctcaca
    14281 gtaaagaaaa cggtatcact ggagtgacac aaaaaacaat taatgcagat gaagaatatt
    14341 ataattactg taggaaatat tttaatttat tgagtttcaa cgagagaata ttggttacaa
    14401 agaaatatta ttctttaaac ataaagcggt tactattaat aggagacaaa tgcaaggctt
    14461 taaaagtaat caagaaggca agagaagaaa aaatttttaa cgaatttctt ttttttgaaa
    14521 tatatgttat tatatcattt tggttttttc tattgtatat atgacaacta tgttcaatta
    14581 aaatttagaa agtgagaaac tattgtgtat actattataa aatcaaatat aaaaaaattt
    14641 agtttattaa cgatatttat tgttgctggt caattattgc taatttatgc agcaactatt
    14701 aatgctctgg tgttgaatga attaattgcg atgaatttag agcggttttt gaaattgtca
    14761 atctaccaaa tgattgtctg gtgtgggata atattccttg actgggtagt gaaaaattat
    14821 caggttgaag tgatccaaga gtttaatcta gagattcgaa atagagttgc cacagacatc
    14881 tctaactcta cctatcaaga atttcatagt aaatcatcag gaacatatct ttcgtggcta
    14941 aataatgatg ttcagacttt aaatgatcag gcttttaaac aacttttttt agtaataaaa
    15001 ggaatttctg gtactatatt tgcagttgtg actcttaatc actatcattg gtcattgact
    15061 gtagccacct tgttttcatt aatgattatg ctacttgtac caaaaatctt tgcatcgaaa
    15121 atgcgagaag ttagtctaaa tttaactaac caaaatgaag cttttttaaa atctagtgag
    15181 actatattga atggatttga tgtgttagca tccttgaatc ttttatatgt attgcctaag
    15241 aaaattaaag aagcaggaat tttattaaag atggttatac aaagaaagac aactgtagaa
    15301 acgttagcag gcgctattag cttctttctc aatatttttt ttcagatatc tctcgttttt
    15361 ttaacaggct atcttgcaat aaaaggaata gtgaaaattg gtactattga agcaatagga
    15421 gcactaacag gtgttatttt tacagcgcta ggtgaattag gaggtcaatt atcctctatt
    15481 attggtacga agcctatttt tttaaaattg tattcaatta atccaattga gtcaaataaa
    15541 atgaatgata tcgaaccaaa tgaggtgaat agagattttc cgttatatga agcaaaaaat
    15601 atttgctata agtatggaga taaagaaata ttaaaaaact taaatttttg ttttcaacgt
    15661 aatgaaaagt atttaatttt aggtgaaagt ggaagcggga aatctacatt attaaaatta
    15721 ttgaatggct ttttgagaga ttatagtgga gaattgcgat tctgcgggga tgatataaaa
    15781 aaaacctcct atttaaatat ggtttcgaat gttctatatg tagatcaaaa agcttatttg
    15841 tttgaaggta cgattagaga taatatttta ttggaagaaa attatactga tgaagaaata
    15901 ctacagtctt tagagcaagt tggtttgagt gtaaaagatt ttcctaataa cattttagat
    15961 tattatgttg gtgatgatgg gagattactg tcaggagggc agaaacaaaa aattacttta
    16021 gctagagggc taattagaaa taagaaaata gtattaattg acgagggaac ttctgctatc
    16081 gataggagaa cttcgttagc gattgaacgt aagatattag atagagagga tttgactgtc
    16141 attattgtta cccatgctcc gcatccggaa cttaaacaat attttactaa gatatatcaa
    16201 tttccaaagg attttattta agaatatagg ggagggaaac atgagattct attgtgaagc
    16261 ttatgaggta attactgaag agaaaaaagt atactttaat gatattgagt tagaagttaa
    16321 gtattcctct gtcgaagaat tgtttataat atgtgagaaa ttattagata agaaaaaagt
    16381 atcatttttt tatgtagatg agaaaccctt aaggtatctg ttatttgact atatatttct
    16441 actagttcta gctaaaaaaa tatcccaatc ttatgaactg caccccaaaa gttagattta
    16501 ttctgtctaa cttttggggt gcagttcatt ttgcatagtt ttggcattca tcctagatat
    16561 ccatataaaa tgtctgagat tgttgaaatg tctaaaaaaa tatcagatct aaacgataaa
    16621 aaagaatttc acaaaaagaa ctgtgagttg aggtttcaag atagtgttct tgataacata
    16681 aatagtgtcc cgagtacaac tcatttattt agttgtttaa atattcaaac attagtaaaa
    16741 attattttat atatttttcg atacaacagt tgtatgaaaa tatttaattt tagagataaa
    16801 gagcttatct tttttaataa gattgtaaat gggctgatac aaaatattga ggagaattta
    16861 gaggatgata ttgaaaggat attgaaatat ttatatatat gtttatttaa cgaaatattc
    16921 attattaaga ataaggtgaa tttttttgat gatgtggaat ttaatcagac acttagtgag
    16981 tttttagata aattgtagag ggaatggtta tgagtaacaa tactaagtat atctttttat
    17041 ctccacattt agatgacgca attttttcct gtggtgatta tatttcaaaa ttaacttcag
    17101 aaggagaaat tgtacttgtt attactatat tttctggtta ccctttgagc caacagctac
    17161 agccctctgc taagcaattt cataagttgt gtaaccttgg gaaatatccg attgaagaaa
    17221 ggaagaaaga agacaggtta gcttgtgaac gtctacagtg tgattttaga cacttatcgt
    17281 actatgaatg tctctatagg aaagatagaa atggcaattt tctataccgt catatctata
    17341 gtgaattaaa aaatgaggac acattaaaga atgatatcat aaaagagctg ttaatgcatt
    17401 tagacgacaa atgtgtcgta tattgtccgc tttcacttgg tgaccatgtt gatcatgttt
    17461 ttgtgaatag tattggaagg gcgcttgaat ttatgcgtta taaagtaata tattatgagg
    17521 acttcccata tgtaagtgat agtagtatgg ttagttatat gggaaaaact aaagaattaa
    17581 aaatgtatca agaggaactt gatgaaaaac attatattga tagaatatca tcaatattgt
    17641 gctataaatc tcagattttg ataatctgga aatcagttga aaaactttta aacaatatta
    17701 aagagttgta tttaaggaat ggggcagctt attctattag attttggatt aagaaataag
    17761 gaagtagttt tatgaatata gcagtaattg gtttggggca tgttgggctg gcctacgcgt
    17821 tactatttgc atctaaatat aaagttgttg catatgatat agattctgta aaaataaata
    17881 atttaaaaaa gggcattctt ccatctaaaa atgaagagct tatgaagttt ttttgcgaga
    17941 ataacttaaa tattactttt tttgatacat tttctgaaat taaaaataat attgattatt
    18001 atattattgc gcttccgaca gattatgatg agaaaattgg tagttttaat acatatgaaa
    18061 tcgaacaaac ggtatcgaag attctgaggg taaaacctaa tggaaagatt attttaaagt
    18121 caacagttcc gttcggcttt tcaaacaaat taaaaaggct gtttgataca aaaaatatca
    18181 tttttgtccc tgaatttttg agagaaggtt gttctatata tgataattta tatccaagtc
    18241 gcatagttgt tggagatgag acagttgaag gaagaaaaat tgcagagttg ttcctttcga
    18301 ttagtactca tagtactgcc aatattaaaa atgttatgtt agtttctcct actgaagcag
    18361 aagcaattaa gcttttttct aacacattct tagctctccg tgttgctttt tttaatgaac
    18421 tagattcttt tgctgagagg agaagtttaa atgctgaagt tgtaataaaa ggtgtttgtt
    18481 tagatccaag aattggaaat ttttataata atctttcttt tggatttgga ggatattgtc
    18541 ttcccaaaga tactaaacaa ttaaaaaaag aatttataga aataaatgcc ccagtgatag
    18601 aagcgattga tatttcaaac acaaatagaa aacagttcat agttaaacaa atattggaac
    18661 gaaagccaaa gatagtagga atatataaat tggggatgaa atataattca gataattaca
    18721 aagagtcagc tattttaagt ataattaatg aactattgat tgtcggtata aaaattttag
    18781 tatatgaacc aaatttaaac gttagtatag ataacgtaat ttttgaaaag aattttgaac
    18841 tatttacaaa acaaagtgat ctaattgttg caaatagatg ggataggggc cttgaagctt
    18901 ataaagataa agtttacaca agggggatct ggataagaga ttaactattg atattaaaaa
    18961 ggcaataatt tataaaattt gtacaaaaaa atgttaaatt ttcacatttt catagtatac
    19021 tgtttaatag aaagtaggtt ctttgtaacc gctccattat aaaatccttt cactcttccg
    19081 gtatactatt aggaaaaaca taactggagg atattttgag ctcacacctc atcgttccag
    19141 tggctgttcc ccaagctcgt gtactgccca aaaaagttag acattctatt tagaacgagg
    19201 attgagttct gtattgtaca aggctcggtc cttttagagt cagcttaagg ctggcttttt
    19261 tattttttct aactatcaga atattttgac aaatgaacac aaataatgat ataatatgca
    19321 aaagctagga ggtggtagga tgattgaaaa aatggaattg ggggaatttt acaaggaatt
    19381 acgtttggcg agaaagctca agcagtcaga tgtggcttgt gacgggctaa cagcttctca
    19441 gttgtccaag tttgagctag ggcagtccat gttgtctgct gataagctca tattagccat
    19501 ccaagggatc aatgtgacct ttgatgagtt tgggcacaag ctcaacaact atcaagaatc
    19561 tccacatatg cagattggta gaaaggttgt ggatcgcttt gctcatcaag atatagctgg
    19621 cttagagcaa ctgttggagg aagtcgagca agaacagatg gcagagactt atcgtcgttt
    19681 gaatgctatt gtaatcaaag atgccatcca ctcactagat aaaagctacc cactagcaga
    19741 ggaggatagc gagtttttga ccacctatct ctatgctatc gagtcttgga cctggtttga
    19801 actctatctc ttttgcaata ctctgccctt cttgagcaat caagatctga tctttttatc
    19861 aacctcctta ctcgaaaaat ccaaagaatt taaagagtta gtacacaatc gattgtatat
    19921 gaagcaagga ctcttaaata tcatctcaga gctcatggag cgcaaacttt tttcttatat
    19981 cccaatcttt gaagccgagc tggagagtat gctccgtcca tacgatgttt ttgagaaagt
    20041 gttgtggcag tttttaaaga agatgagcat tttccttcaa actaagggaa gcaatcaaaa
    20101 agagattgaa cactttatcc aatctctgca agtattagaa aatccacaat taatagccct
    20161 ttttgaattg cgtttgcagc aatacaaaaa gcttatcgat tagttagaaa tggcaaaata
    20221 ataataatct cagcaacgat tttcaataat gttaaaattt gtttcttcat atttctccac
    20281 tcctcattag tagtaacttt attataccag agaagtaatc aaattcacca aaatcgcaaa
    20341 tctgaaattt ctataagaaa aaatatcaaa tatgcgattt tctaaaataa gccaattttc
    20401 gtgttatact gtacttgtaa agcacttgaa gcaaatccta ggtcacagaa gtggtttaca
    20461 aatgaagata attgaaggag tgtaaaagat gttaaattta caatttgcag aaacaatgga
    20521 attgacagaa gctgagttgg agatagttta tggaggggaa tttgggaata atgctgttat
    20581 cccagccggt gcttggggag ggttcgggac accttggtca atcactaatt tctggaagaa
    20641 aaattttaac gatagacctg actttgattc tgaccgccgt cgttattaat atccaaagcc
    20701 cttgcctttt cttgggtagg ggctgtcttt atttcatagt ataggaggaa atagaatgaa
    20761 aaagataatc tcacaccgct actttattat agtttttcta ctagttattg ctgaccagaa
    20821 gttcagtgtt ctagttttac gcagcgacct tgttactggg ctatctgact ttgcctatta
    20881 tctgtcggat atgatgttga attttcttgt ggttttattt gctcttattg ctatgatttg
    20941 gtcggggaaa tggcagaaaa tcaatagtag aaagtttaaa ggatcctatc ttttctattc
    21001 cttcctagct ctgcttgctt ttgttgtttg gaatttcgtt accttttttc ttttcccacc
    21061 tactcgaaat gaaatttctt atcaacatgc tgctccgact tttacagggg ctacagcatt
    21121 tttgatgtat tttttctatc ctgtgattgc aggtcccatt tttgaagaga taatccatcg
    21181 gggattggtg atgacagctc tggaaaaagg aaagaaatgg gggctagatg tacttggctc
    21241 tgctatttta tttggggtct cgcacattag taatcacggt tgggtcttga cagactttgt
    21301 cttctatatg ggtggaggtc ttatatttgc agtcttattt agaatgacaa agtcaattta
    21361 ttggcctatt ggactgcata tagtctacaa tggtattggt cagcttttga tgttactgta
    21421 attttggttg ttagtagtat cgcggtctat tcttggggaa agattcccat ttgaacttaa
    21481 gaaggagttg taatgaaagt atttcttcaa aatagagatt ttaggcaatt aactatcaac
    21541 cagtggattt caacgcttgg ggatacgatt ttttatctgg ccttcctgaa ttatgtggca
    21601 gatgcgtctt ttgccccttt ggcgatttta cttatcacga tttcagaaac ccttccccaa
    21661 gttctacaaa tctttctggg agttttggcg gattttcaac atcatcgtgt cttgaaatac
    21721 acagtcatta gttttacaaa atttttgctt tactctatag tgtccctatc gctttcaggg
    21781 cagtcttttt ccttgtttct agtaaccttt atttgtatga ttaacctctt gtctgacaca
    21841 ttgagttatt tttcaggcgc catgctcact ccgattttca ttagaattat tgggcaagat
    21901 catctggcag aagctattgg atttaaacag tcaactgtta gtttagtgaa aacaatcagt
    21961 aatatcctag gaggagtctt actaggtatt ctatccatcc agtttatttc cttactgaac
    22021 gctcttacct ttttaatcgc atttttaggt atcctcttca taaaaactga cctcttgaaa
    22081 gtagaaaaaa cgattagcta tcaagaagga ctctctgtaa aatccttttg ccagcatttg
    22141 ctccaatcat cgaaattgat atggaatatg aataaggtgc tcttggtttt gtttattatc
    22201 tctactagtc aagcagtgat aaatgttaca gttcctgtct ctactctatt tttgaggaat
    22261 caaccctttt tgaatttaca aacaggtcaa tctcttgcct tgctatccac ttttgaactg
    22321 tcagccctga ttgtcggaag ccttgtaagt ggctatctgc aacataccat ttccataaaa
    22381 acagctctct atgcctcact tgtcatccag ttgcttcttc tagtaggatt tgccacagtt
    22441 cgttttgact ggattctcat ctttagtacc ttggatgcct ttttcgcagg tgttctctct
    22501 cctagattgc aagaactcgt ctttaaacaa atacctgagg agtcaatggg agcagttcaa
    22561 tcttctatca gtgccattac ggttgtttta cctagcttat ttacaatatc tttggtaacc
    22621 attgctacta gctttggaac tctggcagtt agctttgttt tattgctatt tcttctagtt
    22681 gcctttgtta tgcttctgaa tattcgtgaa agtatttagt ggagaatttc tataatatat
    22741 gtttgagact ggattgccag tctttttatg atggtcaaaa tcaacagact ttttctcttt
    22801 tctgaatgtg
//
LOCUS       DBEJAQ010000034        22629 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.034, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000034 DBEJAQ010000000
VERSION     DBEJAQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.034"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            43..753
                     /locus_tag="ACL297_001423"
     CDS             43..753
                     /locus_tag="ACL297_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071677.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISALYSE
                     LDYNKYWYILHTLIYMLMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIMSFLLFLPIAIFMQICCI
                     RFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVCIWIMG
                     FEFFLLPKLIKLKEQNLV"
     gene            1295..1516
                     /locus_tag="ACL297_001424"
     CDS             1295..1516
                     /locus_tag="ACL297_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0071678.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            1962..2286
                     /locus_tag="ACL297_001425"
                     /pseudo
     CDS             1962..2286
                     /locus_tag="ACL297_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            2414..3643
                     /locus_tag="ACL297_001426"
     CDS             2414..3643
                     /locus_tag="ACL297_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGR0071679.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3663..6359
                     /gene="ppc"
                     /locus_tag="ACL297_001427"
     CDS             3663..6359
                     /gene="ppc"
                     /locus_tag="ACL297_001427"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGR0071680.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    6442..6659
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6772..7776
                     /gene="trpX"
                     /locus_tag="ACL297_001428"
     CDS             6772..7776
                     /gene="trpX"
                     /locus_tag="ACL297_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0071681.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7845..8711
                     /locus_tag="ACL297_001429"
     CDS             7845..8711
                     /locus_tag="ACL297_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071682.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8708..9466
                     /locus_tag="ACL297_001430"
     CDS             8708..9466
                     /locus_tag="ACL297_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071683.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9556..11316
                     /gene="dnaG"
                     /locus_tag="ACL297_001431"
     CDS             9556..11316
                     /gene="dnaG"
                     /locus_tag="ACL297_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGR0071684.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            11319..12428
                     /gene="rpoD"
                     /locus_tag="ACL297_001432"
     CDS             11319..12428
                     /gene="rpoD"
                     /locus_tag="ACL297_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGR0071685.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            12442..12771
                     /locus_tag="ACL297_001433"
     CDS             12442..12771
                     /locus_tag="ACL297_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGR0071686.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12886..13929
                     /gene="cpoA"
                     /locus_tag="ACL297_001434"
     CDS             12886..13929
                     /gene="cpoA"
                     /locus_tag="ACL297_001434"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HGR0071687.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13945..15270
                     /locus_tag="ACL297_001435"
     CDS             13945..15270
                     /locus_tag="ACL297_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGR0071688.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            15301..15477
                     /locus_tag="ACL297_001436"
     CDS             15301..15477
                     /locus_tag="ACL297_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGR0071689.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15536..15670
                     /locus_tag="ACL297_001437"
     CDS             15536..15670
                     /locus_tag="ACL297_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HGR0071690.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15734..17044
                     /gene="obgE"
                     /locus_tag="ACL297_001438"
     CDS             15734..17044
                     /gene="obgE"
                     /locus_tag="ACL297_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGR0071691.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            17054..17212
                     /locus_tag="ACL297_001439"
     CDS             17054..17212
                     /locus_tag="ACL297_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071692.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            18299..19558
                     /locus_tag="ACL297_001440"
     CDS             18299..19558
                     /locus_tag="ACL297_001440"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGR0071693.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            19574..20125
                     /locus_tag="ACL297_001441"
     CDS             19574..20125
                     /locus_tag="ACL297_001441"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0071694.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            20118..21395
                     /gene="spxR"
                     /locus_tag="ACL297_001442"
     CDS             20118..21395
                     /gene="spxR"
                     /locus_tag="ACL297_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HGR0071695.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            21411..22271
                     /locus_tag="ACL297_001443"
     CDS             21411..22271
                     /locus_tag="ACL297_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGR0071696.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            22496..>22629
                     /locus_tag="ACL297_001444"
     CDS             22496..>22629
                     /locus_tag="ACL297_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGR0071697.1"
                     /translation="MSQSIVPVEIPQYCRFDSKKRNGILFNVRIANLKFTFFRLDFLR
                     "
BASE COUNT         6970 a         3834 c         4975 g         6850 t
ORIGIN      
        1 aacgaaagaa gttttgagat gaaactttaa taggtaataa aaatgaagtt acttaaaaat
       61 gaattgatta agagtaaaat atttttattt attattgttg acatctgtat tcagattcta
      121 gtcattctag ctataaaaac atatatttta gatatttcgg cactatactc agagcttgat
      181 tataataagt attggtatat tttacacact ttaatatata tgctaatgat ttttcctata
      241 caaattttgt atcaaaatct aagagaggca ttgattgaag ataataataa tggttggaat
      301 atcatggtga ttaacactaa taatctcgtg aaaatcatct atataaaagt aacgataaat
      361 atcgtgagat gttttatttg ttattttgtt tatacaattt tttctttaat tcaattaggt
      421 ggtatgggaa ctgatatgtt attaaccaat ataatatttc ccaatataat gtcatttctt
      481 ttatttttac ctatagctat ctttatgcaa atttgctgta taagatttga ttccatttta
      541 gcaaaggcct tacctaatat tttgctgata cttattgttt taatcacgtt tcagagtgat
      601 tggaatatat ttatacctgc aacttattat tatacagaaa ttcagtcaac tacaaactta
      661 ggtattaaat tattagtttg tatctggatt atgggatttg aatttttcct tcttccaaaa
      721 ctaataaaat tgaaagaaca aaatctagtt taaatgttgc tcgaaaatta aataattgaa
      781 tacgattaac aatgaaacaa tacaacgtga gtcatgactt caaagtaatt gattttatga
      841 ttctggtttc ttacgcttaa ttaagcaagt tgtattcact ttatcaaatt gttattactc
      901 taaaaagcgt ctaacagcta tagagtgtct gtttgcttct ctttactttt attttaaata
      961 attcattaac cttccgtaag tgcccaataa taaggtgtgt tgaagaagtc tgagataaaa
     1021 tagttctcag tcacaaaaaa gctatagatt cccattggtg aacagctccc tgtcaagtag
     1081 acagtaaaat aatataaaat cttaagcgac ctgattcctg aattccagtg gagtcaggtt
     1141 gttttttaac cgtctttctt aatattgtct tacctataat agatatgctt atgagatatg
     1201 tttaataaat taattttagt tcaaaatgtc cagttaggac actttgaatg atttcttagt
     1261 tttatatatt atacttttct caggagaaag ctagatgtac agacgtttga gagatttgag
     1321 ggaagatcac gatttgaccc aaaaacaaat agctaaaata ctttcgttta caaactcagc
     1381 ttatgctaaa attgaacggg gtgagcatac gttaacagca gatgtattgg taactctctc
     1441 aaacttttac gatgtcagta cagactacct attggaattg acagattttc ctgataaaat
     1501 tcgctttaga aaataatctc ctcaatttca tagagtgtga aaatgaggag attttttatt
     1561 tgttctttga aaattaaata gtccaaaatg gtactttcct catttgtgga gcaaatttga
     1621 atggctcgcc atgataagag cgattttaaa accatcaata aaatagagcg atactttata
     1681 tgccatgata caaatgatat acaatgatac ttctgaccgt tcaggctgcc atcgtaaaag
     1741 agcagcaagt gaaattctta tgatgacttc atcagtcatg ccacgttaaa tgtgtgagtt
     1801 tgttagagaa acgcaattaa tcctcaaaag gttccccgaa ccttttgagt tccacagacg
     1861 catcacgtgg agtgtgtaag cttgttgcta aaagcgtaaa aaccttggaa cgaaaggaat
     1921 aatagacttc ctgcgaaaca aaaatacaat acaataaaac tatggatgat gaagcaagta
     1981 aacaattaag cgatagccgt ttcaagagcc ttgtaggtgt tcagcgcacg agatgttagc
     2041 tgtgttaaaa acagcttatc aacgcaacgt aaacgcgcaa aaggtggacg aaaaaccaaa
     2101 ttaagcctag acgatctcct catggtaact attcaataca tgcgagaata gcgcacttat
     2161 gaacaaattg cggctgattt tggcattcac gaaagcaact taatccgtcg gagccaatgg
     2221 gttgaagtaa ctcttgttca aaatggtttt acgatttcaa attctgccct aattatgaaa
     2281 aacagtaaaa ttcgaaggat tgtaaggtaa gagttttttt ctttctgaaa aaatggtata
     2341 atagcaatca aaactagaaa ataaaacgga atttggaaca gatttgtctg tatcctagta
     2401 gagtggtgat gctatgaaga ttagtaagag gcacttatta aattattcca tcttgattcc
     2461 ctacttgctt ttatctattt tgggcttgat tgtggtctat tcgaccacca gtgctatttt
     2521 aattgaagaa ggcaagagcg ccttgcagtt ggttcgaaac caaggaatct tttggattgt
     2581 tagtttgata ctgattgcct taatttataa attgagacta gattttttga gaaatgagcg
     2641 actaatcatt ttagttatat taatagaaat gcttttattg ttcttggctc gttttattgg
     2701 tatttccgta aacggggcat acggttggat ttcggttgca ggagtaacta ttcagccagc
     2761 tgagtactta aaaatcatta ttatttggta tttagctcac cgattctcca aacagcaaga
     2821 agaaatagct acttatgatt ttcaagtttt gactcaaaat caatggcttc cccgtgcttt
     2881 taatgattgg cgattcgttc tcctagttct gattggaagt ttgggaattt tccctgattt
     2941 aggaaatgcg actattttag tcttggtttc cttgattatg tatacagtta gtggaatcgc
     3001 ttatcgctgg ttttcaacca ttctggcgct cgtatctgcc gcttctgtct ttgtcttgac
     3061 cactatcagc ctaatcggtg ttgagacctt ttcaaaaatt ccagtatttg gctatgtagc
     3121 caagcgcttt agtgcctttt ttaatccttt tgccgatcgt gctgatgcag gtcaccagtt
     3181 agctaattct tattttgcca tggtcaatgg tggttggttt ggtctaggtc ttggaaactc
     3241 gattgaaaaa cgaggttatt tgccagaagc tcatacagac tttgtctttt ctatcgtgat
     3301 tgaagaattt ggctttgctg gtgccagtct tattttagct ctcttgtttt tcatgatttt
     3361 gcggattatc ttggtcggta ttcgagcgga gaatcctttc aatgccatgg ttgcactcgg
     3421 tgtcggaggg atgatgttgg ttcaggtatt tgtcaatatc ggagggattt cgggcttgat
     3481 tccatctaca ggagtaacct tccccttctt atcccagggt ggaaatagtc ttctagtctt
     3541 atcagtggca gtagcctttg tcttaaatat tgatgccagt gaaaaacgcg ctaagttgta
     3601 ccgagaattg gaaaatcaac caatgaacct tctgttgaag taggataaag aaaggatagt
     3661 ttatgtctct tcaaaaatta gaaaattata gtaataaaag tgttgtgcaa gaagaagtct
     3721 tgattctaac agaattactg gaagatatta ctaaaaatat gcttgcccca gagacctttg
     3781 aaaaaataat acagttgaaa gaattatcaa cgcaggaaga ttatcaaggt ctaaaccgtc
     3841 tagtgactag cttatcaaat gatgaaatgg tctatatttc acgctatttc tctatcttac
     3901 ctcttttgat caatatttca gaggatgtgg atttagctta tgaaatcaat catcaaaata
     3961 atattgatca ggactattta ggtaaattat ctacaacgat taaattggta gcagaaaagg
     4021 aaaatgccgt tgagatccta gaacacttga atgttgtccc tgttttgaca gcccatccaa
     4081 cacaagtgca acgcaaaagt atgttggatt taacaaatca tattcatagt cttttgcgta
     4141 aataccgtga tgttaagttg gggttgatca ataaagataa atggtacaat gatttgcgtc
     4201 gttacatcga aattatcatg cagacagaca tgattcgtga gaaaaaatta aaagtgacta
     4261 acgaaatcac gaatgctatg gaatattata acagctcctt tttgaaagct gtacctcatt
     4321 tgacgacgga gtataagcgc ttagcgcaag cgcatggtct gaatttaaaa caggctaaac
     4381 caatcaccat gggtatgtgg ataggtggtg accgtgatgg aaatccattt gttacagcag
     4441 agaccttgaa gcagtctgca ctcactcaat gtgaagtcat catgaactac tatgataaaa
     4501 agatttacca actttatcgt gaattttctc tttcaactag cattgtcaac gtcagcaagc
     4561 aagtcagaga aatggctcgt caatccaagg ataactcgat ttaccgcgaa aaagagcttt
     4621 accgtcgtgc cttgtttgat attcaatcaa aaattcaggc aactaaaacc tatctgattg
     4681 aggatgaaga agttgggact cgttatgaaa ccgccaatga tttctacaag gatttgattg
     4741 ccattcgaga ttctctacta gaaaataagg gtgagtcctt gatttcaggt gattttgtgg
     4801 aattattgca ggcagtagag atatttggtt tttacctagc atcaattgat atgcgacaag
     4861 actctagcgt ctatgaagcc tgtgtggcag aactcttgaa atcagcagga attcattctc
     4921 gttatagcga gttgagcgaa gaagaaaagt gtgaccttct cttgaaagaa ttagaagaag
     4981 atccccgaat tctttctgcg actcacgcag aaaaatcaga attattagca aaagaattag
     5041 ctatttttaa gacggctcgt gttttgaaag ataagttggg agatgatgtc atccgtcaga
     5101 ccatcatttc acatgcaacc agcctttctg atatgctaga attagctatt ctgttaaaag
     5161 aagtaggact ggtggatacg gaaagggcgc gtgttcagat tgttcccctt tttgaaacaa
     5221 ttgaagactt ggatcattca gaagaaacaa tgagaaaata tctttctctt agccttgcca
     5281 aaaaatggat tgactcacga aataactacc aagaaatcat gcttggctac tctgacagta
     5341 ataaagatgg cggttacttg tcatcatgtt ggaccctcta caaggctcaa caacaattga
     5401 ctgctattgg agatgaattt ggcgttaagg ttaccttctt ccatggtcgt ggtggtactg
     5461 tcggtcgtgg tggtgggcca acctatgaag ccattacatc tcaaccgctc aagtctatca
     5521 aggatcgtat ccgcttgacg gagcagggtg aagtaattgg gaataaatac ggtaacaaag
     5581 acgccgctta ctataacctt gaaatgctag tatcggcagc tattaaccgt atgattactc
     5641 agaagaagag cgataccaat accccaaatc gttatgaagc cattatggat caagtagtgg
     5701 accgtagtta cgatatctac cgtgatttgg tctttggtaa tgagcatttc tatgattatt
     5761 tcttcgagtc aagtccaatc aaggctattt caagttttaa tattggttct cgtccagccg
     5821 ctcgtaagac tattactgaa atcggtggtt tgcgtgccat cccttgggta ttctcatggt
     5881 cacagagtcg tgttatgttc cctggatggt acggggttgg ttcaagcttc aaggaattta
     5941 tcaataaaaa tccagagaat attgctatct tacgagatat gtaccaaaat tggcctttct
     6001 tccaatcgct tctttcaaat gttgatatgg ttttgtcaaa atcaaatatg aatattgctt
     6061 ttgaatatgc taaactttgt gaagacgagc aagttaaggc catctatgag actattttaa
     6121 atgaatggca agttactaag aacgttatct tggctattga aggacatgac gaactcttag
     6181 ctgacaatcc atatctaaaa gctagtctgg attaccgtat gccttacttt aatattctca
     6241 actatattca gttagagttg attaaacgcc aacgtcgtgg agaattgtcc agtgatcaag
     6301 aacgattgat tcatatcacc atcaacggaa ttgcgacagg attgcgtaat tcaggttgat
     6361 aattttcaag agtgaatgct aaaagtgaat atcaaaaaaa ttctaataga ctattgacaa
     6421 gtagtttaaa aatgatataa tttaaccatt cagaaaagta atcatacaaa ctttttagag
     6481 agtctgtggt agctgaaaac agataagtgg cgatgatgaa aattgggctg aatgctattt
     6541 agaatttgaa attataaaaa ttcggtaagc acaccttaca gtgcatctcg ttattgcgag
     6601 actgagcgat agggaaattc cctataattg aggtggtacc gcgcatcgac gtcctcacac
     6661 aagttttttg tgtgaggatt tttttgatgg aggttagtat ggaaagaaaa cgatggcgtc
     6721 gcttgtttag ataagtgaaa tatgttaaag gaaataaaaa ggagaaacag aatgaaaaat
     6781 aaacgtttaa ttggaattat tgctgcatta gcagtcttag tagcaggaag cttgatttat
     6841 tcttcaatga ataaatcaga agctcagaat aataaggatg agaagaaaat aactaagatt
     6901 ggtgtgcttc aatttgtgag ccatccatcc cttgatttga tttataaagg gatccaagat
     6961 ggacttgcag aagaaggata taaagatgat caagttaaaa ttgattttat gaactcagaa
     7021 ggtgaccaaa gtaaggttgc gacaatgagt aaacaattgg ttgcaaatgg gaatgacctt
     7081 gtggttggta tcgcaacacc agcagcccaa gggttggcta gtgcaacaaa agacctaccg
     7141 gttatcatgg ccgctattac agacccaatt ggtgctaact tggttaaaga tttgaaaaaa
     7201 ccaggtggca acattacagg ggtatctgac cacaatccag ctcaacaaca agttgaactc
     7261 atcaaggctc tgacaccgaa tgtgaaaaca atcggagctc tttactcaag tagcgaagac
     7321 aattcaaaaa cacaggtcga agaatttaag gcttatgctg aaaaagcagg tctgacagtg
     7381 gaaacatttg cagttccttc aacaaatgaa attgcctcaa ctgtcactgt tatgactagc
     7441 aaggtagatg ctatttgggt tccaattgat aacaccattg catcaggatt tccaacggtt
     7501 gtctctagca atcaaagttc taagaaacca atttatccca gtgcgacagc tatggtagaa
     7561 gtaggtggtt tggcatcagt tgtaattgac caacatgacc ttggtgtggc aacaggtaaa
     7621 atgattgtgc aagtcttgaa aggtgcaaaa ccagccgata ccccagtcaa tgtcttttca
     7681 actggtaagt cagtcatcaa taaaaaaata gcacaagaac taggtattac tattcctgag
     7741 tctgttctca aagaagcagg acaagtcatc gaataaataa ggtagggagg aggagactct
     7801 tcccattttt taaaaagatg aatatagaaa ggattaaata aaatatgatt gtttccatta
     7861 tttctcaagg atttgtctgg gctattctag gtctgggaat ctttatgaca tttaggattt
     7921 taaactttcc agatatgacg acagaaggtt ccttccctct tgggggagct gttgctgtca
     7981 ctttgataac caaaggcgtg aacccatttt tagcgacact tgttgctgta ggagcaggtt
     8041 gtttggctgg aatggcagca ggccttcttt atacaaaagg gaagatccca accttgctat
     8101 cagggatttt ggtgatgact tcttgtcact caatcatgct cttgattatg ggacgtgcga
     8161 atttaggcct gcttggaacc aagcaaattc aggatgtttt gccttttgat tcggatttga
     8221 atcaactctt gacaggtctc atctttgtga gtattgttat tgctctcatg ctctttttct
     8281 tggacactaa actcggacaa gcctatattg ctacagggga taatcctgat atggctagaa
     8341 gtttcgggat tcatactgga cgcatggagc tcatgggctt ggtcttatca aatggtgtga
     8401 ttgcccttgc aggtgccctc attgctcagc aagaaggtta tgccgatgtg tctcgaggga
     8461 tcggggttat cgttgtgggg cttgcaagtt tgattattgg agaagttatt ttcaagagtt
     8521 tgagcttggc agagcgtttg gttactatcg ttgtaggttc tatcgcttat caatttttag
     8581 tgtgggcagt tatcgcactt ggctttaata caagttacct tcgtttatac agtgccttga
     8641 ttttagcagt ctgcctcatg attccaacat ttaagcaaac aatcttgaaa ggagccaagt
     8701 taagcaaatg acagcaattg tagaattaaa aaatgcaacc aaaatcgtta aaaatggctt
     8761 tgatgaagaa aagattattt taaatgatgt ttccttagaa atttttgaac gggactttat
     8821 cacgattttg ggcggaaatg gtgctggaaa atcaaccctc tttaacacta tagcagggac
     8881 cttatcacta actagtggaa ctatccgtat tttaggtgaa gaactcacta agttttcacc
     8941 cgagaagcgt gccaagtacc tgtctcgtgt cttccaagat ccaaagatgg ggacagctcc
     9001 ccgtatgacg gtcgctgaaa atcttttaat cgccaagttt cgtggtgaaa agcgtggatt
     9061 gttaccacga cgcttgacta gctataagga tgaatttcag gcaaccattg aaaaagtagg
     9121 aaatggtctt gagaaacact tgaatacacc gattgagttc ttatcaggtg gacaaagaca
     9181 ggctttgagt ctcttgatgg caaccttgaa gcgacctgaa ttactcctgt tagatgagca
     9241 tactgctgcc ctggatccaa agactagtgt tgctttgatg gaattgacag atgaatttgt
     9301 taagaaagat cagctaacag cccttatgat tactcatcat atggaagatg ctctcaaata
     9361 cggcaatcgc ttgattgtca tgaaagaagg acggattatc caagatttaa accaagaaga
     9421 aaaagcaaaa atgaaaatct ctgattatta tcaactcttt gaataaagta gggaaaatga
     9481 gtgaaatggt ttactttttt tctgaaataa agtatactat ataaagtaaa ctatgataac
     9541 atggaggtat tgtgtatggt tgacaaacaa gtcattgaag aaatcaaaaa caatgccaac
     9601 attgtggaag tcataggaga tgtgatttct ttacaaaagg caggacggaa ctatctaggg
     9661 ctctgtcctt ttcatggtga aaaaacacct tctttcaacg ttgtagagga taagcagttt
     9721 tatcactgtt tcggttgtgg tcgctcaggt gatgtcttta agttcatcga ggagtaccaa
     9781 ggggttccct ttatagaggc agtccaaatc ttaggtcagc gtgttggaat tgaggttgaa
     9841 aaaccgcttt atagtgagca gaagccagcc tcacctcacc aagctcttta tgatatgcac
     9901 gaagatgcag ccaaatttta ccatgcgatt ctcatgacaa cgactatggg cgaagaggcc
     9961 agaaattacc tttatcagcg gggtttgaca gatgaagtgc ttaaacattt ttggattggt
    10021 ttagcacctc cagaacgaaa ctatctctat caacgtttgt ctgatcagta tcgtgaagag
    10081 gatttactgg attcaggcct gttttatctc tcggatgcca atcaatttgt agacaccttt
    10141 cacaatcgca ttatgtttcc cctgacaaat gaccaaggaa aggtcattgc cttctcaggt
    10201 cgtatctggc aaaaaacgga ttcacaaact tctaagtata aaaacagccg atcgactgca
    10261 atttttaaca aaagttacga attatatcat atggataggg caaaaaaatc ttctggaaaa
    10321 gctagtgaga tttacttgat ggaaggattc atggatgtta ttgcagccta tcgggctgga
    10381 atcgaaagtg ctgtggcgtc gatgggaacg gccttgagtc gagagcatgt tgagcatctg
    10441 aaaaggttaa ccaagaaatt ggttcttgtt tacgatggag ataaggctgg gcaagccgcg
    10501 acattgaaag cattggatga aattggtgat atgcctgtgc aaatcgtcag catgcctgat
    10561 aacttggatc ctgatgagta tctacaaaaa aatggtccag aagacttggc ctatctatta
    10621 acgaaaactc gtattagtcc gattgagttc tacattcatc agtacaaacc tgaaaacagt
    10681 gaaaatctgc aggctcagat tgagtttctt gaaaaaatag ctcccttgat tgttcaagaa
    10741 aagtccatcg ctgctcaaaa cagctatatt catattttag ctgacagtct ggcgtccttt
    10801 gattataccc agattgagca gattgttaat gagagtcgtc aggtgcaaag gcagaatcgc
    10861 atggaaggaa tttccagacc gacgccaatc accatgccaa tcaccaagca gttatcggct
    10921 attatgaggg cagaagccca tctactctat cggatgatgg aatcccctct tgttttgaac
    10981 gattaccgtt tgcgagaaga ctttgcattt gctacacctg aatttcaggt cttatatgac
    11041 ttgcttggcc agtatggaaa tcttcctcca gaagttttag cagagcagac agaggaagtt
    11101 gaaagagctt ggtaccaagt tttagctcag gatttgcctg ctgaaatatc gccgcaggaa
    11161 cttagtgaag tagagatgac tcgaaacaag gctctcttga atcaggacaa tatgagaatc
    11221 aaaaagaagg tgcaggaagc tagccatgta ggagatacag atacagccct agaagaattg
    11281 gaacgtttaa tttcccaaaa gagaagaatg gagtaataat ggcaacaaaa caaaaagaag
    11341 taacaacatt tgacgtacag gtagcagaat ttatccgtaa tcataagcaa aaagggacag
    11401 caacagatga tgaaatcaat gcaagtctgg ttattccttt taccttggac gctgatggga
    11461 ttgaagatct cttgcaacgg attcaggatg caggaatttc tatcacagat aacgaaggaa
    11521 atccaagtgc gcgtgttctt agcaatgaag aagaaccaga actcagcgat gaggacttga
    11581 ttgggtcaac ttctgctaag gtcaatgacc ctgtccgtat gtacttgaaa gaaatagggg
    11641 tcgttcctct cttgaccaat gaagaggaga aagagttggc actggctgtt gaagctggtg
    11701 atatcgaagc caaacaacgt cttgcggaag ccaatcttcg tttggttgtt tccattgcca
    11761 aacgctatgt cggtcgtggc atgcagttcc ttgacttgat tcaagaagga aatatgggct
    11821 tgatgaaggc ggttgacaag tttgactatt ctaaagggtt caagttttca acttatgcaa
    11881 cttggtggat tcgtcaggct atcactcgtg ctattgcgga ccaagctcgt accatccgta
    11941 tcccagttca catggttgaa actatcaata aattggttcg tgaacagcgg aatctccttc
    12001 aagaattggg gcaagatccg acaccagaac agattgctga acgaatggat atgacacctg
    12061 ataaggttcg tgaaatcttg aagattgccc aagaaccagt atctcttgaa actcctatcg
    12121 gtgaagagga cgatagccac cttggagact ttatcgaaga tgaagtgatt gaaaatccag
    12181 tggattatac gactcgtatc gtcttgcgtg agcaattgga tgaaatctta gatactctta
    12241 cagaccgtga agaaaatgtt ctgcgtctac gttttggact agatgatgga aaaatgcgca
    12301 cacttgaaga tgtggggaaa gtctttaacg taactcgtga gcgtatccgt cagattgaag
    12361 caaaggcttt gagaaaacta cgccaaccaa gtcgtagcaa accgcttcgt gattttattg
    12421 aagactaaga gtgaggaaaa tatggcttat acagaagagc aaattgaaaa catcaaaaca
    12481 cggattttaa cagccttgga agaagtcatc gaccctgagt tgggaatcga tattgtcaat
    12541 cttggtttga tctatgagat tcgttttgac ggtgacacag ggcaaacaga gattgacatg
    12601 actttgacaa ctatgggttg tcccttagca gaccttttga cagaccagat ttatgatgct
    12661 atgatagagg ttccagaagt aacggatact gaggttaaat tagtctggta tccagcctgg
    12721 acagttgaaa aaatgagtcg ctatgcccgc attgccctag gcattaagta aacatataaa
    12781 agcatgtgag agactgttgg aaaagcgagg aaatttcccc tcttttcctc tagtctctcc
    12841 tttcttttgc tgattttatt caaagaaaat gatataatag tagttatgga gaaaaagaaa
    12901 ttacgcatca atatgttgag ttcaagtgag aaagtagcag gacagggagt ttcaggtgct
    12961 taccgtgaat tagttcgtct tcttcaccgt gctgccaagg accaattgat tgttacagaa
    13021 aatcttccaa tcgaggcaga tgtgactcac tttcatacga ttgattttcc ctattattta
    13081 tcaaccttcc aaaagaaacg ctcagggaga aagattggct atgtgcattt cttgccagct
    13141 acacttgagg gaagtttgaa aattccattt ttcttaaagg gaattgtgaa acgctatgta
    13201 ttttcttttt acaaccggat ggagcacttg gttgtggtca atcctatgtt tattgaggat
    13261 ttggtagcag ctggtattcc acgtgaaaaa gtgacctata ttcctaactt tgtcaacaag
    13321 gaaaaatggc atcctctacc acaagaagag gtagtcagac tgcgcacaga tcttggtctt
    13381 agtgacaatc agtttatcgt agtaggtgct gggcaagttc agaaacgtaa agggattgat
    13441 gactttatcc gtctggctga ggaattgcct cagattacct ttatctgggc tggtggcttc
    13501 tcttttggtg gtatgacaga tggttatgaa cactataaga aaattatgga aaatccccct
    13561 aaaaatttga tttttccagg cattgtatcg ccagagcgga tgcgcgaatt gtatgctcta
    13621 gcggatcttt tcttgttgcc tagttacaat gagctctttc ctatgactat tttagaagct
    13681 gcgagttgtg aggctcctat tatgttgcgt gatttagatc tctataaggt aattttggag
    13741 ggaaattatc gggcgacagc gggtagagaa gagatgaaag aggctatttt ggaatatcaa
    13801 gcaaatcctg ctgccttaaa agatctcaaa gaaaaggcta agaatatttc cagagagtat
    13861 tctgaagagc atctgttaca aatctggttg gacttttatg agaaacaagc cgctttaggg
    13921 agaaagtaaa aagtgaggta atctatgcga attggtttat ttacagatac ctattttcct
    13981 caggtttctg gtgttgcgac cagtattcga accttgaaaa cagaacttga aaagcaggga
    14041 catgctgttt ttatctttac gacgacagat aaggatgtca atcgctacga agattggcaa
    14101 attatccgca ttccaagtgt tcctttcttt gcttttaagg atcgtcgctt tgcctaccga
    14161 ggttttagca aggcacttga aattgctaaa cagtatcagc tagatattat ccatactcag
    14221 acagaatttt ctcttggcct gttggggatt tggattgcgc gtgaattgaa aattccagtc
    14281 atccatacct atcacaccca gtatgaagac tatgtccatt atattgctaa ggggatgttg
    14341 atccggccga gtatggtcaa gtatctggtt agaggtttcc tgcatgatgt ggatggggtt
    14401 atttgtccta gtgagattgt ccgtgacttg ctatctgatt ataaggtcaa ggttgaaaaa
    14461 cgggtcattc ctactgggat tgaattagcc aagtttgagc gtccggaaat caagcaggaa
    14521 aatttgaaag aactgcgtag taaactaggg attcaagatg gtgaaaagac gttgcttagt
    14581 ctttcgagaa tctcctatga aaaaaatatt caagcagttt tagcagcctt tgctgatgtt
    14641 ctgaaagagg aagacaaggt taaactggta gtagctgggg atggccctta tctgaatgac
    14701 ctcaaagagc aagctcagaa cctagagatt caagactcag tcatctttac agggatgatt
    14761 gctcctagtg agacggctct ttactataaa gcggcggatt tcttcatttc ggcatcgaca
    14821 agcgaaacgc aaggtttgac ctacttggaa agcttagcca gtggaacacc tgtcattgct
    14881 cacggaaatc cttatttgaa caacctcatc agtgataaaa tgtttggaac cttgtactat
    14941 ggagaacatg atttggctgg tgctattttg gaagccctga ttgcaacacc agacatgaac
    15001 gagcatacct tatcagagaa attgtatgag atttcagctg agaactttgg gaaacgagtg
    15061 catgagtttt atctggatgc cattatttca aataacttcc agaaagattt ggctaaagat
    15121 gatacggtca gtcagcgtat ctttaagaca gttttgtatc ttcagcaaca ggtggttgct
    15181 gtacctgtaa aaggatctag acgcatgttg aaggcttcaa aaacacagtt gatcagtatg
    15241 agagactatt ggaaagacca tgaagaatag aaagaggaac agctatgaaa aaaacaatta
    15301 atgagaagcg gtcgtgataa aaagattgcg ggtgtttgtg ctggggtggc ccattatctg
    15361 gatatggatc cgactatcgt tcaagtcatt tggggtgttc ttacttgctg ttacggagct
    15421 ggaattgtag cttacattat tttatggatt atcgcaccag tagcaactga ctattgaaaa
    15481 ctagctcaat tcatgctaag ataatagaaa atagaatgta acgaaggaga gaaaaatggc
    15541 atttggagat aatggaaatc gtaaaaaaac tatgtttgag aaaataacct tgtttatcgt
    15601 gattatcatg ctagtagcaa gtttattggg aatttttgca actgcaattg gtgccctcag
    15661 taatctataa aatagattca agaaaattta gtgactggga tttcccagcc cttttttaaa
    15721 gtgagaagaa ataatgagta tgtttttaga tacagctaag attaaggtca aggctggtaa
    15781 tggtggcgat ggtatggttg cctttcgtcg tgaaaaatat gtccctaatg gaggcccttg
    15841 gggtggtgat ggtggtcgtg gaggcaatgt ggtcttcgtt gtagacgaag gactacgtac
    15901 cttgatggat ttccgctaca atcgtcattt caaggctgat tctggtgaaa aagggatgac
    15961 caaagggatg catggtcgtg gtgctgagga ccttagagtt cgagtaccac aaggtacgac
    16021 tgttcgtgat gcggagactg gcaaggtttt aacagatttg attgaacatg ggcaagaatt
    16081 tatcgttgcc cacggtggtc gtggtggacg tggaaatatt cgtttcgcga caccaaaaaa
    16141 tcctgcaccg gaaatctctg aaaatggaga accaggtcag gaacgtgagt tacaattgga
    16201 actaaaaatc ttggcagatg tcggtttagt aggattccca tctgtaggga agtcaacact
    16261 tttaagtgtt attacctcag ctaagcctaa aattggtgcc taccacttta ccactattgt
    16321 accaaattta ggtatggttc gcacccaatc aggtgaatcc tttgcagtag ccgacttgcc
    16381 aggtttgatt gaaggggcta gtcaaggtgt tggtttggga actcagttcc tccgtcacat
    16441 cgagcgtaca cgtgttatcc ttcacatcat tgatatgtca gctagcgaag gccgtgatcc
    16501 atatgaggat tacctagcta tcaataaaga gctggagtct tacaatcttc gcctcatgga
    16561 gcgtccacag attattgtag ctaataagat ggacatgcct gagagtcagg aaaatcttga
    16621 agaatttaag aaaaaattgt ctgaaaatta tgatgaattt gaagagttac cagctatctt
    16681 cccaatttct ggattgacca agcaaggtct ggcaacactt ttagatgcta cagctgaatt
    16741 gttagacaag acaccagaat ttttgctcta cgacgagtcc gatatggaag aagaagctta
    16801 ctatggattt gacgaagaag aaaaagcctt tgaaattagt cgtgatgacg atgcgacatg
    16861 ggtactttct ggtgaaaaac tcatgaaact ctttaatatg accaactttg atcgtgatga
    16921 atctgtcatg aaatttgccc gtcagcttcg tggtatgggg gttgatgaag cccttcgtgc
    16981 gcgtggagct aaagatgggg atttggtccg cattggtaaa tttgagtttg aatttgtaga
    17041 ctaggagact ggtatgggag ataaaccgat atctttccga gatgcggatg gtaattttgt
    17101 ttccgccgca gacgtttgga atgaaaagaa attggaagaa ctatttaatc gtctcaatcc
    17161 aaatcgtgcc ttgagattgg cacgaactaa aaaggaaaat ccatctcagt aaagaagcta
    17221 aaaaatcccg tgcctcatca gacacgggat tttgtggtac gacaggcatg tatagcaaac
    17281 tgaatctgga atagcacagc atatcttcta aaatattgct ataaattgat ttgaatttca
    17341 aaattaaatt gttttattct tatttcaatt tgttatagta tatctgatgt caaagttctc
    17401 ggcgagtcaa atagtgattc ccaagcctga ctatcgtgag gtagcggatt aaaatggtct
    17461 ggggatagac cgttttaagt ctgatgctgg aaataagaat tgtcagaaga agggatagcg
    17521 aaatcgtggc tctacgaaca ggaacgtgat aataaggcgt atatagcgaa taagagggcc
    17581 tcaaactcta aagtccaaaa aggtagtcgt aacctatatg cgtaaatcat gagagtaatt
    17641 gaattcgtac taagattttc tatttttact gtaacctttt aacgtcctca tatcttgtat
    17701 acacgaggaa agatgtacga cttatcccgt gaggtctatc actataaaga gaaaacgaca
    17761 gatagaagtg atcctgagtc acggttatct ttaaaataga ttgtctgata ggatgctagc
    17821 ctgaaaacgt cttgttcatg aaatatagga cggtatgtat aaaacgcttc tgtgaactga
    17881 gagaaggggg agaagttctt gctaaaattt agttgaacag ccgtattccg atacttagat
    17941 aagagatcta gtcttagctc ctactcagtt tcaggggata aaaaaggggc aatagcgatt
    18001 cgagaaagat tatactcttc gaaaatctct tcaaatcacg tcaatatcgc cttgccgtat
    18061 gtgtaggata ctaactacgt cagttccatc tacaatctca aaacagtgtt ttgagcaacc
    18121 tgcggctagt ttcctagttt gatctttgat ttttattgag tattagtaat tcagttacta
    18181 actcgtcaac tctgatttat ccaataaaat tgaaaaggat ggaaaaaagg ataaatttat
    18241 gatatacttt attttgaaga ccttattaga aatcttgaaa gagtattgaa aacttagaat
    18301 gagaaaaatt gttatcaatg gtggattacc actgcaaggt gaaattacta ttagtggtgc
    18361 taaaaatagt gttgtggcct taattccagc tattatattg gctgatgatg tggtgacttt
    18421 ggattgtgtt ccagatattt cggatgtagc cagtcttgtc gaaatcatgg aattaatggg
    18481 agctactgtt aagcgttatg acgatgtctt ggagattgat ccaagaggtg ttcaaaatat
    18541 tccaatgcct tatggtaaaa ttaacagtct tcgtgcatct tactattttt atgggagcct
    18601 cttaggccgt tttggtgaag cgacagttgg tctaccggga ggatgtgatc ttggtcctcg
    18661 tccgattgac ttacacctta aggcgtttga agctatgggt gccactgcta gctacgaggg
    18721 agataacatg aagttatctg ctaaagatac aggacttcat ggtgcaagta tttacatgga
    18781 tacggttagt gtgggagcaa cgattaatac gatgattgct gcggttaaag caaatggtcg
    18841 tactattatt gaaaatgcag cccgtgaacc tgagattatt gatgtagcta ctctcttgaa
    18901 taatatgggt gcccatatcc gtggggcagg aactaatatc atcattattg atggtgttga
    18961 aagattacat gggacacgtc atcaggtgat tccagaccgc attgaagctg gaacatatat
    19021 atctttagct gctgcagttg gtaaaggaat tcgtataaat aatgttcttt acgaacacct
    19081 ggaagggttt attgctaagt tggaagaaat gggagtgaga atgactgtat ctgaagacag
    19141 catttttgtc gaggaacagt ctaatttgaa agcaatcaat attaagacag ctccttaccc
    19201 aggctttgca actgatttgc aacaaccgct tacccctctt ttactaagag cgaatggtcg
    19261 tggtacaatt gtcgatacga tttacgaaaa acgtgtaaat catgtttttg aactagcaaa
    19321 gatggatgcg gatatttcga caacaaatgg tcatattttg tacacgggtg gacgtgattt
    19381 acgtggggcc agtgttaaag cgaccgactt aagagctggg gctgcactag tcattgctgg
    19441 gcttatggct gaaggtaaaa ctgaaattac caatatcgag tttatcttac gtggttattc
    19501 tgatattatc gaaaaattac gtaatttagg agcggatatt agacttgttg aggattaaac
    19561 cgtagaggtg tttatgaata tttggaccaa attagcaatg ttttcttttt ttgaaacgga
    19621 tcgcttgtat ttgcgtcctt tcttttttag tgatagtcag gacttccgcg agatagcttc
    19681 aaatccagaa aatcttcaat ttattttccc aacgcaggca agtctggaag aaagtcaata
    19741 tgcactggcc aattacttta tgaagtcccc tttgggagtg tgggcaattt gtgaccagaa
    19801 aaatcaacaa atgattggtt ctattaaatt tgagaagtta gatgaaatca aaaaagaagc
    19861 tgagcttggc tattttttga gaaaagatgc ttggtcgcaa ggatttatga cagaggttgt
    19921 tagaaaaatt tgtcagcttt cttttgagga atttggctta aaacaattat ttatcattac
    19981 ccaccttgaa aatgaagcta gccaaagagt tgctcttaag tctggattta gtttgttccg
    20041 tcagtttaag ggaagtgatc gttacacaag aaaaatgcgg gattatcttg aatttcggta
    20101 tgtaaaagga gagttcaatg agtaagcatc aggaaattct aagctatttg gaggaattac
    20161 cagtaggtaa aagggtcagt gttcgtagca tttcgaatca tctaggagtt agtgatggaa
    20221 cagcctatcg agctattaaa gaagctgaaa accgtggaat tgtggagacc cgtcctagaa
    20281 gtggaacaat tcgtgttaaa tcccagaaag ttgctataga gagattaacg tttgctgaaa
    20341 ttgcagaagt gacttcttct gaggttctgg ctgggcaaga aggtttagag agagagttta
    20401 gtaagttttc aattggtgcc atgactgaac aaaatatctt gtcttacctt catgatgggg
    20461 ggctcttgat tgtcggagac cgaacccgta ttcagttgct agccttggaa aatgaaaatg
    20521 cagttctggt tacaggggga tttcaggttc atgatgatgt gcttaaactg gccaatcaaa
    20581 aagggattcc tgttctaaga agtaagcatg atacctttac cgtcgcgacc atgatcaata
    20641 aagccttgtc aaatgtccaa atcaagactg atattctgac agttgagaaa ctttatcgcc
    20701 ctagtcatga gtatggtttt ctgagagaga cagatacagt taaagattat ttggacttgg
    20761 ttcgtaagaa tcgtagcagc cgtttccctg ttatcaatca acatcaggtc gttgttggtg
    20821 ttgtaaccat gagagacgct ggtgataaat caccaagcac gacaattgat aaggttatgt
    20881 ctcgtagtct attttgggtt ggattatcga caaatattgc caatgtgagt caacggatga
    20941 tcgcagaaga ctttgaaatg gtaccagttg ttcgaagcaa tcaaactttg cttggcgttg
    21001 tgacgcgacg agatgtcatg gagaagatga gccgttccca agtttcggct ctaccaactt
    21061 tttctgagca gattggacaa aagctctctt atcaccatga tgaagtagtc attacagtgg
    21121 aaccctttat gctagaaaaa aatggagttt tggctaatgg tgtattggca gaaattctga
    21181 cccacatgac ccaagattta gttgttaata gtggtcgcaa tctcattatc gagcagatgc
    21241 tgatctactt tttgcaggct gttcagatag atgatatatt gcgcattcag gcacggatta
    21301 ttcatcatac gagacggtca gctataattg attacgatat ttatcatggt caccagattg
    21361 tttcaaaagc aaatgtgact gttaaaatta attagaaact aggagaaaag atgataacat
    21421 taaaatcagc tcgtgaaatc gaagctatgg acaaggctgg tgattttcta gcaagtattc
    21481 atataggctt acgtgatttg attaagccag gcgtagatat gtgggaagtt gaagaatatg
    21541 tccgccgtcg ttgtaaagaa gaaaatttcc ttccacttca gattggggtt gacggtgcca
    21601 tgatggacta tccttatgct acctgttgct ctcttaacga tgaagtggct cacgctttcc
    21661 ctcgtcatta tatcttgaaa gatggtgatt tgctcaaagt tgatatggtt ttgggaggtc
    21721 ccattgctaa atccgacctg aatgtctcaa aattaaactt caacaatgtt gaacaaatga
    21781 aaaaatacac tcagagctat tctggtggtt tagcagactc atgttgggct tatgctgttg
    21841 gtacaccgtc cgaagaagtc aaaaacttga tggatgtaac caaagaagct atgtacaagg
    21901 gtattgagca agctgttgtt ggaaatcgta tcggtgatat cggtgcggct attcaagaat
    21961 acgctgaaag tcgtggttac ggtgtagtgc gtgatttggt tggtcatggt gttggcccaa
    22021 ctatgcacga agaaccaatg gttcctaact atggtattgc aggtcgtgga ctccgtcttc
    22081 gtgaaggaat ggtcttaacc attgaaccaa tgatcaatac aggcgattgg gaaattgata
    22141 cagatatgaa aactggttgg gcgcataaga ccattgacgg tggattgtca tgtcagtatg
    22201 aacaccaatt tgtcattacg aaagatggac ctgttatctt gactagccaa ggtgaagaag
    22261 gaacttatta ataaaaagtg aaaagactac tggaagttta ttttgataaa aaatccagta
    22321 gatcttttca taataaaacg cattgtatca agtgttagga gctgatatca tgcgtttttc
    22381 tgcttttaag attttttcca actctgtttg taagcgcatc ataacaaagg gtctaggatt
    22441 cagggctctc ctcctatata ctattagtaa agtaaaacta agagaggata ttttagtgtc
    22501 gcagtctatt gttcctgtag agattccaca atattgtcgt tttgattcta aaaagagaaa
    22561 tggaattctg tttaacgttc gtattgccaa tcttaaattt acctttttta gattagactt
    22621 cctgcgaaa
//
LOCUS       DBEJAQ010000035        22507 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.035, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000035 DBEJAQ010000000
VERSION     DBEJAQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.035"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            632..2107
                     /locus_tag="ACL297_001445"
     CDS             632..2107
                     /locus_tag="ACL297_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGR0071698.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(2079..2204)
                     /locus_tag="ACL297_001446"
                     /pseudo
     CDS             complement(2079..2204)
                     /locus_tag="ACL297_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2184..2788)
                     /locus_tag="ACL297_001447"
                     /pseudo
     CDS             complement(2184..2788)
                     /locus_tag="ACL297_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846390.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3044..3904
                     /gene="speE"
                     /locus_tag="ACL297_001448"
     CDS             3044..3904
                     /gene="speE"
                     /locus_tag="ACL297_001448"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGR0071699.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            3901..5160
                     /locus_tag="ACL297_001449"
     CDS             3901..5160
                     /locus_tag="ACL297_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HGR0071700.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            5160..6287
                     /gene="nspC"
                     /locus_tag="ACL297_001450"
     CDS             5160..6287
                     /gene="nspC"
                     /locus_tag="ACL297_001450"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HGR0071701.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6284..7369
                     /gene="aguA"
                     /locus_tag="ACL297_001451"
     CDS             6284..7369
                     /gene="aguA"
                     /locus_tag="ACL297_001451"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HGR0071702.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7379..8254
                     /gene="aguB"
                     /locus_tag="ACL297_001452"
     CDS             7379..8254
                     /gene="aguB"
                     /locus_tag="ACL297_001452"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HGR0071703.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8435..9244)
                     /locus_tag="ACL297_001453"
     CDS             complement(8435..9244)
                     /locus_tag="ACL297_001453"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGR0071704.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            9516..9737
                     /locus_tag="ACL297_001454"
     CDS             9516..9737
                     /locus_tag="ACL297_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HGR0071705.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCSRGTYDLGYIGGGNHLCKGSAARF"
     gene            9791..10462
                     /locus_tag="ACL297_001455"
     CDS             9791..10462
                     /locus_tag="ACL297_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGR0071706.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(10514..10711)
                     /locus_tag="ACL297_001456"
     CDS             complement(10514..10711)
                     /locus_tag="ACL297_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGR0071707.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            10704..11612
                     /locus_tag="ACL297_001457"
     CDS             10704..11612
                     /locus_tag="ACL297_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGR0071708.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11609..12070
                     /gene="lspA"
                     /locus_tag="ACL297_001458"
     CDS             11609..12070
                     /gene="lspA"
                     /locus_tag="ACL297_001458"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGR0071709.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            12060..12947
                     /locus_tag="ACL297_001459"
     CDS             12060..12947
                     /locus_tag="ACL297_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGR0071710.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            12950..14833
                     /gene="cbpE"
                     /locus_tag="ACL297_001460"
                     /gene_synonym="pce"
     CDS             12950..14833
                     /gene="cbpE"
                     /locus_tag="ACL297_001460"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HGR0071711.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            14934..16043
                     /gene="proB"
                     /locus_tag="ACL297_001461"
     CDS             14934..16043
                     /gene="proB"
                     /locus_tag="ACL297_001461"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGR0071712.1"
                     /translation="MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHEL
                     ILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILL
                     TQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
                     ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQG
                     SIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGAS
                     ALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            16053..17315
                     /locus_tag="ACL297_001462"
     CDS             16053..17315
                     /locus_tag="ACL297_001462"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGR0071713.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            17319..18116
                     /gene="proC"
                     /locus_tag="ACL297_001463"
     CDS             17319..18116
                     /gene="proC"
                     /locus_tag="ACL297_001463"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGR0071714.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            18232..18870
                     /gene="tmk"
                     /locus_tag="ACL297_001464"
     CDS             18232..18870
                     /gene="tmk"
                     /locus_tag="ACL297_001464"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGR0071715.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTNAVLFDGMGLAK"
     gene            18867..19757
                     /locus_tag="ACL297_001465"
     CDS             18867..19757
                     /locus_tag="ACL297_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGR0071716.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            19797..20114
                     /gene="yabA"
                     /locus_tag="ACL297_001466"
     CDS             19797..20114
                     /gene="yabA"
                     /locus_tag="ACL297_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HGR0071717.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            20117..20986
                     /gene="rsmI"
                     /locus_tag="ACL297_001467"
     CDS             20117..20986
                     /gene="rsmI"
                     /locus_tag="ACL297_001467"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGR0071718.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            21048..21176
                     /locus_tag="ACL297_001468"
                     /pseudo
     CDS             21048..21176
                     /locus_tag="ACL297_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
     gene            21213..21731
                     /locus_tag="ACL297_001469"
     CDS             21213..21731
                     /locus_tag="ACL297_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HGR0071719.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            21840..22485
                     /locus_tag="ACL297_001470"
                     /pseudo
     CDS             21840..22485
                     /locus_tag="ACL297_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
BASE COUNT         6797 a         3985 c         5166 g         6559 t
ORIGIN      
        1 tcagctagtt gaaccatcta atttttagga gggctgggtg gctaacttca ttatagaact
       61 ttcatttacg aacatatagt aaaatgaaac aagaacagaa caaatcgatc aggacagtaa
      121 aatctatttc taacaatgtt ttagaagcag aggtgtacta ttctagtttc aatctactat
      181 atttttgttt tttatcaaaa aatactttac aagttcttaa aaacatgata tagtaataaa
      241 gcttagaaaa tgagatgatg ttttctagca aatataaacc cgagtaaaaa atgcctacgg
      301 acaggcaggg ttgaatgccg aagcgtggtt gaaaagccac attattgata gggttaaaag
      361 cctactttta taagttgatg ttaggacact tgtcctaatt cataaatttt tagtgtggtg
      421 aaagcacacg tcatcttgtg aaacgatcaa taaagtacgt aatatttgct actagagagt
      481 taggaaacat cgggaacaga catactcaac agaaaccaaa ataaacacgt cagaagattg
      541 cagagcaggt gaaaacctgc tcttttttca tgagtcaacc tttagttcct tagttttcat
      601 aaggtcctaa aaatattgaa aggagtatgt tttgaaagag ttagatcaaa accaagcccc
      661 aatttatgag gccttggtga agttacgcaa gaaaaggatt gttccctttg atgttccagg
      721 tcacaagcgt ggacggggaa atccagaact tgtcgaactc ttaggagaaa aatgtgtagg
      781 cattgatgtc aattcgatga aacccttgga taatttaggc catcctattt cgattattcg
      841 tgatgcagag gagctggctg cagatgcttt tggagctagc catgcctttc taatgattgg
      901 tggaacaact tcatcggtgc agactatgat tctggcaacc tgcaaggcag gagataagat
      961 tattctgcca cgaaatgtcc ataaatctgc tatcaatgcg ttggttctat gtggtgccat
     1021 tcccatctat atcgagatga gtgtagatcc taagattggt atcgctttag gtcttgaaaa
     1081 tgaccgagta gcacaggcca taaaggacca tccagatgct aaggctatcc taatcaacaa
     1141 tcctacttac tacggcatct gttcagacct aaaggggttg acagaaatgg ctcatgaagc
     1201 tggcatgatg gttttagtag atgaagccca tggagcgcat ttgcatttca ctggtaaact
     1261 tccaatttct gctatggatg caggagctga tatggcagca gtttccatgc ataagtctgg
     1321 tgggagtttg acacaaagtt cgcttctttt aatcggggag cagatgaatt ctgaatacgt
     1381 tcgtcagata attaacctga ctcagtctac atctgcctct tacttgttga tggctagttt
     1441 ggatatttca cgtcgcaact tggcccttcg tggtaaagag tcgtttgaga aagtcattga
     1501 gctatctgag tatgcccgcc gtgaaatcaa tgctatcggt ggctactatg cctactcaaa
     1561 agagttaata gacggtgttt cggtttgcga ttttgacgta actaagctgt cagtttacac
     1621 tcagggtatt ggcttaacag gtatcgaggt ttatgacctc ttgcgagacg aatacgacat
     1681 tcagatcgag tttggtgata tcggcaatat cttggcctat atttccatcg gcgaccgcat
     1741 ccaagacatc gagcgcttgg ttggtgctct ggctgatatt aagagactct attcaagaga
     1801 tggaaaagat ttgatagcag gagaatatat tcagcccgag ttggtgctgt ctccacaaga
     1861 agccttctat tcagagagaa aaagtttgac cttagatgag tctgttggac aggtctgtgg
     1921 agaatttgtc atgtgctatc ctccagggat tcctatcttg gctcctggtg aacgcattac
     1981 acgagaaatt gtagactata tccaattcgc caaggaacgt ggttgctccc tccaagggac
     2041 ggaagatcca gaggtcaatc atatcaacgt tattaagaga aagacaaact ataagaaaag
     2101 tcaatagttt tatctaaact atttcttatt tcaatttgat gatttggcga tgattttaga
     2161 gcacggcaaa aagcccttga attagaagcg gtcaatcgct taatttctat cagcttatca
     2221 aatcctgcct caatcctttt ctgaggatta gggtagcgtg tcaagagttg gtaggtatat
     2281 tctgaatgct ttccaacgat tttatccaac tcaggaaaga tgatatcaag acaacgagtg
     2341 tattgtactt tccaatcaga ctgtttttct tgagacgatg aatatgtcta gccagtattt
     2401 ttagttctac ttgccgatta tcgtgttgaa attgttcacg attggggtca gaaagaagtt
     2461 taagagcgat gccatgagcg tctttcttat ccgttttagt cttgtgaagt gataatgatt
     2521 tggcaaattc cttgatgagc aaaggattgt aggtgtaaac tttatatcct tgttcatgca
     2581 ggaagttcag tagattaaag gcataatgtc cggtattttc aagagcgatg agacagtctt
     2641 ggttgagttg tcgaagagac agatctaaga gttcaaaacc agctttatta tttgaaaaag
     2701 tgagtggttt aagaacagtt tttcctggaa cattcaaggc tgtaacatcg tgtttatttt
     2761 tagcgacatc aatgcccaca taaagcatgg gagtacctcc agatgtagta tttcaagtct
     2821 acttggttat ccacgaattt tttgccttgt taccttagac gagataaaac gtctatgcgt
     2881 tatcaaactc attaccaatt gaaacaaaaa actgtggtta gagcctttcg gaaatcgtca
     2941 agcgattgga gggaataaac taatccacag tggcttattc caagtatacc acttgggctt
     3001 tggcagtagc taactgcgct aaatataata taaggagaaa tagatggatt tatggttttc
     3061 tgaagttcat actccagatg tcaaattgtc tctgagaaca gccaagcaac tttacgctgg
     3121 aaaaagtgaa tggcaggata tcgaagtctt ggatacgcca gcttttggga aaatactgat
     3181 tttaaatggc catgtcttgt tctcagatgc ggatgatttc gtctacaatg aaatgaccgt
     3241 tcacgttccc atggctgtcc acccaaatcc aaagaaagta ttggttattg ggggtggtga
     3301 cggcggtgtt gcccaagtat taacactcta tcctgaactg gaacaaatcg atatcgtgga
     3361 accggatgag atgttggtcg aggtctgtcg tgagtatttc ccagactttg ctgcagggct
     3421 agatgatcct cgtgttacca tttactacca aaatgggcta cgctttttgc gaaactgcga
     3481 agatgattac gatattatca tcaacgatgc gacagatcca tttggccata cggaaggact
     3541 ctttaccaag gaattctacg gcaatagtta tcgagctctg aaggaagacg gcatcatgat
     3601 ttaccagcat gggagtccct tctttgacga ggatgagtcg gcctgccgaa gcatgcaccg
     3661 caaggtcaat caagcctttc caatcagtcg ggtttatcag gcccatattc caactagccc
     3721 agctggctat tggttgtttg gatttgcatc gaaaaaatac caccctgtca aagattttga
     3781 caaggaaggt tggaaaaaac gccagctttt cacagaatac tacactgcaa acttacacgt
     3841 gggatccttt atgttgccca agtatgttga ggacatttta gaagaagagg aaggaaaaaa
     3901 atgagtcgtt tactagttat tggttgtggg ggcgttgccc aagttgctat ttcaaagatt
     3961 tgtcaagata gcgaaacatt tacagagatt atgattgcta gccgtaccaa gtcaaaatgc
     4021 gatgacttga aagcgaagct agaaggcaaa acaagtacta aaattgaaac tgcagcactt
     4081 gatgctgaca aggttgaaga agtgattgcc ctgattgaaa gctacaaacc agaagctgtt
     4141 ttgaatgtag ctctgcctta tcaagattta accattatgg atgcttgttt ggcaacaggt
     4201 gttcactata tcgatacagc caactacgaa gcagaagaca cagaagaccc tgagtggcgt
     4261 gctatctacg aaaaacgttg taaggaactt ggttttacag cctactttga ctactcatgg
     4321 cagtgggctt atcaagagaa attcaaagaa gcaggcttga ctgctcttct tggttctggt
     4381 tttgacccag gtgtaactag tgtcttttca gcttatgccc tcaaacacta ttttgatgaa
     4441 atccattata tcgacatttt agactgtaat ggcggtgacc acggttatcc atttgcaacc
     4501 aactttaatc cagaaattaa tctccgtgag gtttctgcgc caggttctta ctgggaagat
     4561 gggaaatggg tcgaagtcga agctatgtct atcaagcgtg agtatgattt ccctcaagtt
     4621 ggacaaaaag atatgtatct ccttcaccat gaagaaatcg aatcattggc caagaacatt
     4681 ccaggtgtca aacgcattcg tttctttatg acttttggtc aatcttactt gacgcacatg
     4741 aaatgtcttg aaaatgttgg actccttcgt acggatacca ttaactttaa cggccaagaa
     4801 attgttccaa ttcaattttt gaaagccttg cttccagatc ctgctagcct tggcccacgt
     4861 actgtaggta aaaccaatat tggatgtatc tttacaggtg tcaaagatgg cgttgaaaag
     4921 actatctaca tctacaatgt ttgcgaccat caagagtgct atgcagaagt cgggtcacaa
     4981 gcaatttcat acacgacagg cgttccagcc atgattggga caaaattagt catgaacgga
     5041 acttggaaac aagctggagt gtataacctt gaggagttag atccagatcc attcatggaa
     5101 gctttgaatg agtatggttt gccatgggtt gtggttgaaa atccacaaat ggtggactaa
     5161 tgaagttaga acaagtacca acaccagcct atgttattga cttggccaag ttagaagcta
     5221 attgccgcat tctacaatat gtacaagaag aggccggttg caaggtcttg cttgcccaga
     5281 aggcatattc cctctacaaa acttatccct tgattagcca gtatctatca ggtacgacag
     5341 ctagtggact ctatgaggcc aaattggcaa gggaagaatt tcctggtgaa gtccatgttt
     5401 ttgcaccagc tttcaaggat gaggacatgg aggaattgct tgggataaca gaccatatcg
     5461 tctttaactc agagagacag ttgcgtaagc atggggctcg ttgtcgagag gctggtgtca
     5521 gtgttggttt gcgcctcaac cctcagtgtt caactcaagg agatcacgcg ctctatgacc
     5581 cttgtgcacc tggttctcgc tttggagtta ctatagacaa gattccgagt gatttgctag
     5641 atttggttga cggacttcat tttcataccc tttgcgagca gggagcagat gatttacaaa
     5701 caactttgaa agcagtagaa gaacagtttg gtccctactt acatgaggta aaatggctca
     5761 atatgggtgg tggtcatcat attacaagag aaggttacga tgtggatttg ctgatttcag
     5821 aaatcaagcg tatccgaaaa acttacaatc ttgaaatcta tatcgagcct ggtgaagcca
     5881 ttgcgcttaa tgcgggttat ttagcaactg aggtattaga tattgtagaa aacggtatgg
     5941 aaatcttggt tttagacgcc tctgcgacct gccatatgcc tgatgtactt gagatgccct
     6001 atcgtccacc tttgagaaat ggctttgagt cacaggaaaa agcccatacc tacagacttt
     6061 cttctaatac ctgtctgact ggcgatgtga ttggtgatta tagttttgaa aatccagtcc
     6121 aaatcggaga cagactttat tttcaagaca tggccattta ttcttttgtc aaaaataata
     6181 cctttaatgg tattggattg ccaagtctct atctcatgga cgaacaggga gactgtagct
     6241 tactcaaagc ttttggctat caagacttta aagggagatt atcatgatgg acagtccaaa
     6301 aaaattaggc tatcacatgc cagcagagta cgaaccccat catggtaccc tcatgatatg
     6361 gccgactcga ccaggatcat ggccttttca aggaaaggct gctaaaagag catttactca
     6421 gatcatcgag accatagcag aaggggaaag agtctatctt ttggtggagc aggcctatct
     6481 atctgaagcc caatcctatc ttggagacaa ggttgtttat ttagacattc ccaccaatga
     6541 tgcctgggcg cgtgatactg gcccaaccat tctcgtcaat gataaaggta agaaattagc
     6601 cgtggattgg gcctttaatg cttggggagg cacctatgat ggtctttatc aagattatga
     6661 agaggatgac caagtagcca gtcgttttgc tgaggccttg gaaaggcctg tctatgatgc
     6721 taaacctttt gtactggaag gaggcgcaat ccatagcgat ggtcaaggaa ctattctcgt
     6781 aactgaaagt tgcttgctta gtcctggtcg caatcctaac ctgactaaag aggagattga
     6841 aaacacatta ttagaaagtc ttggtgctga aaaagttatt tggcttcctt atggtattta
     6901 tcaggatgaa accaatgaac acgtcgataa tgttgttgcc tttgttggtc ctgctgaggt
     6961 tgttttggct tggacagatg acgaaaatga tccccagtat gccatgtcaa aagcagatct
     7021 cgaactctta gaacaggaaa cagatgcaaa aggttgtcac ttcaccattc ataaattgcc
     7081 tatccctgca gttcgacaag ttgtgacaga agaagatttg ccaggctaca tctatgaaga
     7141 aggagaagaa gagcgatacg caggtgaacg actagcagct tcctacgtaa acttttatat
     7201 cgccaacaag gctgtcttgg ttccacagtt tgaggatgta aacgaccaag tggccttaga
     7261 tatcctcagc aagtgtttcc cagaccgtaa agttgtcgga ataccagcca gagatattct
     7321 cttaggtggt ggcaatatcc actgtatcac ccaacaaatt ccagaatagg agaaaaagat
     7381 gagaaatgta agagttgcaa ccattcagat gcaatgcgct aaggatgtgg caacaaatat
     7441 ccaaaccgca gagcgtttag tacgtcaggc tgctgagcaa ggagcccaaa ttattctctt
     7501 gcccgagttg tttgaacatc cctatttctg tcaggaacgt cagtatgact actaccagta
     7561 tgcccaatct gtagcggaaa atactgccat tcagcatttt aaggtgattg ctaaggaact
     7621 acaagttgtt ttaccaatca gtttctatga aaaagatggt aatgtcttgt ataactctat
     7681 tgccgtcatt gatgcagatg ggggagtgct gggcgtttat cgaaagaccc atataccaga
     7741 tgaccattat tatcaagaaa aattctattt cacgcctggt aacactggtt tcaaggtctg
     7801 gaatactcgc tatgctaaga ttggtatcgg tatctgttgg gatcaatggt tccctgaaac
     7861 agcccgctgt cttgcattga atggtgctga attgctcttt tatcctacag ctatcggttc
     7921 agagccaatt ttggatacag atagttgtgg tcactggcaa cgtactatgc aagggcacgc
     7981 agcagcgaat attgttccag tcatcgcagc caatcgttat ggtttagagg aggttactcc
     8041 tagtgaggaa aatggcggac agagttccag tcttgacttc tacggttcct cctttatgac
     8101 ggatgaaaca ggagctattc tagaacgagc tgaaagacaa gaagaagctg ttctgttagc
     8161 tacttatgac ctagacaagg gagcaagtga acgtctaaac tggggcttgt ttcgagatag
     8221 aagaccagaa atgtatagac aaattacaga ttagtgtggg agaaatgaga gattcattct
     8281 gctagactaa cttcttatta gcaactataa gatactatgg catctagtaa atcgattttt
     8341 atgattcgct attcttgtct attgattagt ccgtatttta aaatattagc aaaaaagcaa
     8401 atagcagtaa cttctgtcta tttgcttttc ttttttatag aatatatttc tcaatagcac
     8461 gcgcaacgcc gtcttcttcg ttgcttgagg taacggcatc cgcaagagat ttgacataat
     8521 cgctggcatt tcccattgca atcccaagcc ctgcaaactg gagcatttcg atatcgttat
     8581 tagcatcgcc catggccata atctctgagg aatcaatctt caaaatctca gctagtcgtg
     8641 aaagagcagt agcctttgtc gttccaagcg gcattgcttc ataaatgaca ggctgcgaac
     8701 gaactccact gaatcgttgg caaagctctt cagcaaaacg ctgctcaaaa tcgtctgttt
     8761 gttcttttgt tcctaaaaac ataccttgga acatccggaa ctttccacta gtcgcttctt
     8821 caagagaaat ttcagtcagg tctgaaaata ctagtttagc atcattttca ataacttgat
     8881 tgggtttgcc accgagaaca aaataatgtg actcgtcaaa aagtgtcaac tgaacatcac
     8941 tcttttcagc aaggtcatag aggtattcga tgtcagctgg actcagttct ttccagtcaa
     9001 ctagactcca atcactggtc tggtgagttg aacaaccgtt gttaacaata atatattcgt
     9061 tctggaggtc aagctccagt tttttgtagt aggggaggac accgaaaagg gggcgacccg
     9121 tacagagaac cagtttgaca cctttttcaa tggctttgtg aatagcagta atgtgagctt
     9181 gtgggatttc cttggcttca ttgaggaggg tgccgtccat atccaaggct agtagtttaa
     9241 tcataggtct tccttcttta tcttttgcta ttattatagc atattttgga gaagaaattg
     9301 atagaaagct tgagactaat tgattttata gtttaagatg ttttgatgac aattcatgat
     9361 ttgaagagga tatttggcaa agatatgcta tactatgttt gtcaatgttg caactagaca
     9421 aattaaaaaa ccaacttaat ataatagttt ttttgtaagt aggtatgagt atcagattac
     9481 tcaactaatc tgaagaataa tggaggaaat atatcatgat tttaatgaca aaaaatataa
     9541 atctaacaaa tgaagaatta gagctgatac aaggtggagc agattcatat ggtaaaaatc
     9601 ctaatggtag gtacgattgg gaaatagaac cagtattaac tctgccggtt catggatttt
     9661 gttccagagg cacctatgat ttaggatata ttggaggagg caatcatctt tgcaaaggaa
     9721 gtgctgcgag attttaagta aaatttatta gtaatatgaa gaaacaagtg gagaaagcag
     9781 agaatttaat atgaaaaaac gggctattca aattttacta gcattgtcct taatttttta
     9841 caaatcaact tggttttgga gagttttcaa tcacctcgca aagccctatc taccagcaag
     9901 tcgtgaattt tttcagattc tgcttttgat ggagagcgga gttcttttct tagcggtcat
     9961 ctatctactg gtttttgcag gaaagaaaat ttttcatttc aagtggcagc tgaggtactt
    10021 catctacctt ttactgggct acatcatttc atatatgtct gacttcctct tttcgtattt
    10081 catatccctg tcttcaaatc agatttcttt gaatgaaacg atagaaatga tggggagaca
    10141 ggagttccct tatttcttgc tcatcgtttg cttcatcgcc cctattgctg aggaattgat
    10201 ttatcgaggt gtgcttatga caaccttttt caaaaactca ccttggtacg gagatgtttt
    10261 gctttctgct attattttcg gttatattca tatcaatttt gctttaactc ctcttgcttt
    10321 tttcatttat gctagtggag gtctgatttt agctctattg tatcgcatga ctaaaaatct
    10381 ctactatcca atactagttc atattctcat caatatcact gccttctggg atgtttggtt
    10441 actcctattt tcaggaagtt agcttactaa aaaaatgtcg gaattttccg gcattttctt
    10501 ttttcacaaa tagtcaacgt ttttctttcc gatactgaag tggtgtgtag ccacttattt
    10561 ttttgaattg attttgaaaa taagattggc gtgagaaagg cagatagtga agatagttaa
    10621 gaagaatagg atgttctttt ttcctttttg gaaaacttct aaaatatggt ataatgaaaa
    10681 gataaagaag ttgggggtag aagatgaaca ttcaacaatt acgctatgtt gtggctattg
    10741 ccaatagtgg tacttttcgt gaagctgctg aaaagatgta tgttagtcag ccgagtctgt
    10801 ctatttctgt tcgtgatttg gaaaaagagt tgggctttaa gattttccgt cggaccagct
    10861 cagggacttt cttgacccgt cgtgggatgg aattttatga aaaagcgcaa gaattggtta
    10921 aaggatttga tatttttcaa aatcagtatg ccaatcctga agaagaaaaa gatgaatttt
    10981 ccgttgctag ccagcactat gacttcttac caccaactat tacggccttt tcagagcgct
    11041 atcctgacta taagaacttc cgtatttttg aatcaactac tgttcaaata ttagatgaag
    11101 tggcgcaagg gcatagtgag attgggatta tctacctcaa caatcaaaat aaaaagggga
    11161 ttatgcaacg ggttgaaaag ttaggtctgg aggtcatcga attgattcct ttccataccc
    11221 atatttatct ctgtgagggt catcctttag cccagaaaga ggaattagtc atggaggatt
    11281 tagcggattt accaacggtt cgtttcactc aagagaaaga cgagtacctt tattattcag
    11341 agaactttgt cgataccagc gctagctcac agatgtttaa tgtgacagac cgtgccacct
    11401 tgaatggtat tttggagcgg acggacgcct atgcgacagg ttctggattt ttagatagtg
    11461 acagtgttaa tggcattaca gttattcgtc tcaaggataa cctagataac cgcatggtct
    11521 atgttaaacg tgaagaagtg gagcttagtc aagctgggac tctcttcgta gaagtcatgc
    11581 aagaatattt tgatcaaaag aggaaatcat gaaaaaaaga gcaatagtgg cagtcattgt
    11641 actgctttta attgggctgg atcagttggt caaatcttat atcgtccagc agattccact
    11701 gggtgaagtg cgctcctgga tccccaattt cgttagcttg acctacctgc aaaatcgagg
    11761 tgcagccttt tctatcttac aagatcagca gttgttattc gctgtcatta ctctggttgt
    11821 cgtgataggt gccatttggt atttacataa acacatggag gactcattct ggatggtctt
    11881 gggtttgact ctaataatcg cgggtggtct tggaaacttt attgacaggg tcagtcaggg
    11941 ctttgttgtg gatatgttcc accttgactt tatcaacttt gcaattttca atgtggcaga
    12001 tagctatctg acggttggag tgattatttt attgattgca atgctaaaag aggaaataaa
    12061 tggaaattaa aattgaaact ggtggtctgc gtttggataa ggctttgtca gatttgtcag
    12121 aattatcacg tagtctcgcg aatgaacaaa ttaaatcagg ccaggtcttg gtcaatggtc
    12181 aagtcaagaa agctaaatac acagtccaag agggtgatgt cgtcacttac catgtgccag
    12241 aaccagaggt attagagtat gtggctgagg atcttccgct agaaatagtc taccaagatg
    12301 aggatgtggc tgtcgttaac aaacctcagg gaatggttgt gcacccgagt gctggtcata
    12361 ccagtggaac cctagtaaat gccctcatgt atcatattaa ggacttgtcg ggtatcaatg
    12421 gggttctgcg tccagggatt gttcaccgta ttgataagga tacgtcaggt cttctcatga
    12481 ttgctaaaaa cgatgatgcg catctagtac ttgcccaaga actcaaagat aaaaagtctc
    12541 tccgcaaata ttgggcgatt gttcatggaa atctgcctaa tgatcgtggt gtaattgaag
    12601 cgccgattgg ccggagtgaa aaagaccgta agaaacaggc tgtaactgct aaagggaagc
    12661 ctgcagtgac gcgttttcac gtcttggaac gctttggcga ttatagctta gtagagttgc
    12721 aactggagac agggcgcact catcaaatcc gtgtccacat ggcttatatc ggccattcag
    12781 tcgctggtga tgaggtctat ggtcctcgca agactttgaa aggacatgga caatttcttc
    12841 atgccaagac tttaggtttt actcatccga gaacaggtaa gaccttggaa tttaaagcag
    12901 atatcccaga gatttttaag gaaaccttgg agagattgag aaagtaagaa tgaaaaagaa
    12961 attaactagt ttagcacttg taggcgcttt tttaggtttg tcatggtatg ggaatgttca
    13021 ggctcaagaa agttcaggaa ataaaatcca ctttatcaat gttcaagaag gtggcagtga
    13081 tgcgattatt cttgaaagca atggacattt tgccatggtg gatacaggag aagattatga
    13141 tttcccagat ggaagtgatt ctcgctatcc atggagagaa ggaattgaaa cgtcttataa
    13201 gcatgttcta acagaccgtg tctttcgtcg tttgaaggaa ttgggtgtcc aaaaacttga
    13261 ttttattttg gtgacccata cccacagtga tcatattgga aatgttgatg aattactgtc
    13321 tacctatcca gttgaccgag tctatcttaa gaaatatagt gatagtcgta ttactaattc
    13381 tgaacgtcta tgggataatc tgtatggcta tgataaggtt ttacagactg ctgcagaaaa
    13441 aggtgtttca gttattcaaa atatcacaca aggggatgct cattttcagt ttggggacat
    13501 ggatattcag ctctataatt atgaaaatga aactgattca tcgggtgaat taaagaaaat
    13561 ttgggatgac aattccaatt ccttgattag cgtggtgaaa gtcaatggca agaaaattta
    13621 ccttgggggc gatttagata atgttcatgg agcagaagac aagtatggtc ctctcattgg
    13681 aaaagttgat ttgatgaagt ttaatcatca ccatgatacc aacaaatcaa ataccaagga
    13741 tttcattaaa aatttgagtc cgagtttgat tgttcaaact tcggatagtc taccttggaa
    13801 aaatggtgtt gatagtgagt atgttaattg gctcaaagaa cgaggaattg agagaatcaa
    13861 tgcagccagc aaagactatg atgcaacagt ttttgatatt cgacaagacg gtttagtaaa
    13921 catttcaact tcctacaagc cgattccaag ttttcaagct ggttggcata agagttcata
    13981 tggaaactgg tggtatcaag cgcctgattc tacaggagaa tatgctgtcg gttggaatga
    14041 aattaagggt gaatggtatt actttaacca aacgggtatc ttattacaga atcaatggaa
    14101 aaaatggaac aatcgttggt tctatttgac agactctggt gttgctgcta aaaattggaa
    14161 gaaaatcgct ggaatctggt attattttaa caaagaaaac cagatggaaa ttggttggat
    14221 tcaagataaa gagcagtggt attatttgga tgttgatggc tctatgaaga caggttggct
    14281 tcaatataag gggcaatggt attactttgc tccatcaggg gaaatgaaaa cgggctgggt
    14341 aaaagataaa gaagcctggt actatatgga ttctactggt atcatgaaga caggtgagat
    14401 agaagttgct ggacatcatt actatctaga agaatcagga gctatgaagc aaggctggct
    14461 taaaaaggca aatgattggt atttctacaa gacagatggt tcacgagcta taggttggat
    14521 taaagacaag gataaatggt acttcttgaa agaaaatggt caattacttg tgaatggtaa
    14581 gacaccagaa ggctatactg tggattcaag tggtgcctgg ttagtggatg ttccgatcga
    14641 gaaatctgct acaattaaaa ctacaagtca ttcagaaata aaagaatcca aagaagtagt
    14701 gaaaaaggat cttgaaaata aagaaacgag tcaacatgaa agtgttacaa attcttcaac
    14761 tagtcaagat ttgacatcct caacttcaca aagctctgaa acgagtgtaa acaaatcgga
    14821 atcagaacag tagtagaaaa gaaggtttta gggccttctt tttcctatca actcttttct
    14881 atttcctgtt attcatgtta taatggataa atatgaataa tcggagtgag actatgaaat
    14941 acaaacggat tgtctttaag gtgggtactt cttctctgac aaatgaggat ggaagtttat
    15001 cacgtagtaa ggtaaaggat attacccagc agttggctat gctgcacgag gctggtcatg
    15061 agttgatttt ggtgtcttca ggtgccattg cggctggttt tggagcctta ggatttaaaa
    15121 agcgtccgac taaaattgct gataaacagg cttcagcagc ggtagggcag ggacttttgt
    15181 tggaagaata tacgaccaat cttctcttgc gtcaaatcgt ttctgcacaa atcttgctga
    15241 cccaagatga ctttgtggat aagcgtcgtt ataaaaatgc ccatcaggct ttgtcggttt
    15301 tgctcaaccg tggggcaatt cctatcatca atgagaatga tagtgtcgtt attgatgagc
    15361 tcaaggttgg ggacaatgac actctaagtg ctcaagtagc ggcgatggtc caagcagacc
    15421 ttttagtttt cttgacagat gtggacggtc tctatactgg aaatcctaat tcagatccaa
    15481 gagccaaacg cttggagaga atcgagacca tcaatcgtga gattattgat atggctggtg
    15541 gagctggttc gtcaaacgga actgggggta tgttaaccaa aatcaaggct gcaactatcg
    15601 cgacggaatc aggagttcct gtttatatct gctcatcctt gaaatcagat tccatgattg
    15661 aggcggcaga ggagaccgag gatggttctt actttgttgc tcaagagaag gggcttcgta
    15721 cccagaaaca atggcttgcc ttctatgctc agagtcaagg ttctatttgg gttgataaag
    15781 gggctgcgga agctctctct caacatggaa agagtcttct cttatctggt atcgttgaag
    15841 cagaaggagt cttttcttac ggtgatatcg tgacagtatt tgataaggaa agtggaaaat
    15901 cacttggaaa aggacgcgtg caatttggag catctgcttt ggaggatatg ttgcgttctc
    15961 aaaaagccaa gggtgtcttg atttaccgtg acgactggat ttccattact cctgaaatcc
    16021 aactactttt tacagaattt tagaggtaaa ctatggtgag tagacaagaa caatttgaac
    16081 aggtacaggc tgttaaaaaa tcgattaaca cagctagtga agaagtgaaa aaccaagcct
    16141 tgctagccat ggctgatcac ttagtggctg ctactgagga aattttagcg gctaatgccc
    16201 tcgatatggc agcggctaag gggaaaatct cagatgtgat gttggatcgt ctttatttgg
    16261 atgcagatcg tatagaagcg atggcaagag gaattcgtga agtggttgcc ttaccagatc
    16321 caatcggtga agttttagaa acaagtcagc ttgaaaatgg tttggttatc acaaaaaaac
    16381 gtgtagctat gggtgtcatc ggtattatct atgaaagccg tccaaatgtg acgtctgatg
    16441 cggctgcttt gactcttaag agtggaaatg cggttgttct tcgtagtggt aaggatgcct
    16501 atcaaacaac ccatgccatt gtcacagcct tgaagaaggg cttggagacg actactattc
    16561 atccaaatgt gattcaactg gtggaggata ctagccgtga aagtagttat gctatgatga
    16621 aggccaaggg ctatctagac cttctcattc ctcgtggagg agctggcttg atcaatgcag
    16681 tggttgagaa tgcgattgta cctgttatcg agacagggac tgggattgtc catgtctatg
    16741 tggataagga tgcagacgaa gacaaggcgc tgtctatcat caacaatgct aaaaccagtc
    16801 gtccttctgt ttgtaatgcc atggaggttc tgctggttca tgaaaacaag gcagcaagca
    16861 tccttcctcg cttggatcaa atgctggttg cagatcgaaa agaagctggg ttggaaccaa
    16921 ttcaattccg cctagatagc aaagcaagcc agtttgtttc aggtcaagct gctcaagcac
    16981 aagactttga taccgagttt ttagactata ttctagctgt taaggttgtg agcagtttag
    17041 aagaagcggt tgcgcatatt gaatcccaca gtacccatca ttcggatgct attgtgacgg
    17101 aaaatgctga agctgcagca tactttacag atcaagtgga ctctgcagcg gtgtatgtta
    17161 atgcctcaac tcgtttcaca gatggaggac aatttggtct tggttgtgaa atggggattt
    17221 ctactcagaa attgcacgcg cgtggtccaa tgggcttgaa agagttgacc agctacaagt
    17281 atgtggttgc tggtgatggg cagataaggg agtaagagat gaagattgga tttatcggtt
    17341 tggggaatat gggtgctagc ttggcaaaat ctgtcttgca gactaggacg tcagatgaga
    17401 ttctccttgc caatcgtagt caagctaagg tagatgcttt cattgcagac tttggtggtc
    17461 aggcttccag caatgaagaa atgtttgcag aagcagatgt gatttttcta ggagttaagc
    17521 ctgctcagtt ttctgaactg ctttctcaat atcagaccat ccttgaaaaa agagaaagtc
    17581 ttcttttgat ttcgatggca gctggattga ccttagaaaa actagcaagt cttatcccaa
    17641 gccaacaccg aattattcgt atgatgccta atacccctgc ttctatcggg caaggagtga
    17701 ttagttatgc cttgtctcct aattgcaggg ctgaggacag tgagatcttt tgtcagcttt
    17761 tagccaaggc tggtctcttg gttgaattag gagaaagctt aatcaatgca gcgacaggtc
    17821 ttgcaggttg tggaccagcc tttgtctatc tctttattga ggccttggca gatgcaggtg
    17881 ttcagacggg attaccacga gaaacagcct tgaaaatggc agcccaaact gtggtaggag
    17941 ctgggcaatt ggtcctagaa agccagcaac atcctggagt tttgaaagat caagtttgta
    18001 gtccaggcgg ttcgactatt gctggcgtag caagcttaga agcgcatgct tttcgaggca
    18061 tagtcatgga ggcagttcat caagcctata aacgaacaca agaactaggt aaataagagg
    18121 tagctttgac tgcctctttt atggtggctg aaatgagaag acacaaaaag attgtcacaa
    18181 actcctattt ttttgataga atagaagtag taaaaaagaa atgagttaga catgtcaaaa
    18241 ggatttttag tctctcttga gggaccagag ggagcaggca agaccagtgt tttagaggct
    18301 ctgctaccaa ttttagagga aaaaggagta gaggtgctga cgacccgtga acctggcgga
    18361 gtcttgattg gggagaagat tcgggaagtg attttggatc caagtcatac tcagatggat
    18421 gctaaaacag agctacttct ctatattgcc agtcgcagac agcatttggt ggaaaaagtt
    18481 cttccagccc ttgaagctgg caagttggtc atcatggatc gttttatcga tagttctgtt
    18541 gcctatcagg gatttggtcg tggcttagat attgaagcca ttgactggct caatcagttt
    18601 gcgacagatg gcctcaaacc cgatttgaca ctctattttg acatcgaggt ggaagaaggg
    18661 ctggctcgta ttgctgctaa tagtgaccgc gaggttaatc gtttggattt ggaagggttg
    18721 gacttgcata aaaaagttcg tcaaggctac ctttctcttc tggataaaga gggaaatcgc
    18781 attgtcaaga ttgatgctag tctccctttg gagcaagttg tggaaactac caatgctgtc
    18841 ttgtttgacg gaatgggctt ggccaaatga aacaagatca actaaaggct tggcaaccag
    18901 ctcagtttga ccgttttgtc cgtatcttag aacaagacca gctcaatcac gcctatctct
    18961 tttcaggttt ctttggaagc ttggaaatgg cgcaattttt agctaagagc cttttttgta
    19021 cggataaagt tggcgtctta ccatgtgaga aatgccgaag ttgcaagctg attgaacagg
    19081 aagagtttcc agatgtcacc ttgattaagc cagtcaatca ggtcatcaag acagaacgta
    19141 ttcgggaatt ggtgggacag ttttctcaag cagggattga aagccagcaa caggtcttta
    19201 tcatcgagca agcggataaa atgcatccca acgcagccaa ttctctgctc aaggtcatcg
    19261 aagaacccca gagtgaagtt tatattttct tcttgactag cgatgaggaa aagatgttac
    19321 cgacaatccg aagtcggact cagatcttcc actttaaaaa gcaagaagaa aaacttatct
    19381 tactcttaga acaaatggga cttgttaaga aaaaagcgac tcttttagct aagtttagtc
    19441 aatcgcgagc tgaagcagaa aagttggcac atcaggcaag tttttggacc ttggtcgatg
    19501 aaagtgaacg cctgctgact tggttagtag ctaagaaaaa agaaagttat ctacaggttg
    19561 ccaaattagc caacttggca gatgataagg aaaaacagga tcaggtttta cggattcttg
    19621 aagttctctg tgggcaggac ctcttgcagg taagagtaag agtgattcta caagatttac
    19681 tagaagctag aaaaatgtgg caagctaatg tcagctttca aaatgccatg gaatatctgg
    19741 tcttgaaaga aatataaact caaaaatgaa tgataaagaa aggaaagggc tgttttatgg
    19801 acaaaaaaga attatttgac gcgctggatg atttttccca acagttattg gtaaccttgg
    19861 ccgatgtgga agccatcaag aaaaatctca agagcctggt agaggaaaat acagctcttc
    19921 gtttggaaaa ttctaagttg cgagaacgct tgggtgaggt ggaagcagat gctcctgtca
    19981 aggccaagca tgttcgtgaa agtgtccgtc gcatttaccg tgatggattt cacgtatgta
    20041 atgattttta tggacaacgt cgagagcagg acgaggaatg tatgttttgt gacgagttgc
    20101 tatacaggga gtaggcatgc agattcaaaa aagttttaag gggcagtctc cctatggcaa
    20161 gctgtatcta gtggcaacgc cgattggcaa tctagatgat atgacctttc gagctatcca
    20221 gaccttgaaa gaagtagatt ggattgctgc tgaggatacg cgcaatacag gtcttttgct
    20281 caagcatttt gacatttcca ccaagcagat cagttttcat gagcacaatg ccaaggaaaa
    20341 aattcctgat ttgattggtt tcttgaaagc agggcaaagt attgctcagg tctctgatgc
    20401 cggtttgcct agcatttcag accctggtca tgatttagtt aaggcagcta ttgaggaaga
    20461 aattgcagtt gtgacagttc caggtgcctc tgcaggaatt tctgccttga ttgccagtgg
    20521 tttagcgcca cagccacata tcttttacgg ttttttaccg agaaaatcag gtcagcagaa
    20581 gcaatttttt ggcttgaaaa aagattatcc tgaaacacag attttttatg aatcacctca
    20641 tcgtgtagca gacacgttgg aaaatatgtt agaagtctac ggtgaccgct ccgttgtctt
    20701 ggtcagggaa ttgaccaaaa tctatgaaga ataccaacga ggtactatct ctgagttatt
    20761 agaaagcatt gctgaaacgc cactcaaggg cgaatgtctt ctcattgttg agggtgccag
    20821 tcagggtgtg gaggaaaagg acgaggaaga cttgttcgta gaaattcaaa cccgcatcca
    20881 gcaaggtatg aagaaaaatc aagctattaa ggaagtagct aagatttacc agtggaataa
    20941 gagtcaactc tacgctgcct accacgactg ggaagaaaaa caataaaggg agataggatg
    21001 taataattct gtctgtttct gtttaactta attagtgatg ataatataaa gatgtatcac
    21061 ttggtataga agctttggta ttaagttttt tattaagccc atacggaata ccgatggttg
    21121 gagcagcagt tatagcgttc ttagaaggta taaatagaaa aataaggtca ttttaaatca
    21181 aaggattgat aaatcagaaa gaaggtgatt ttttgcgaac atacgaaaat aaagaagaac
    21241 taaaagctga gatagagaaa acatttgaga aatatatttt agaatttgat aatattccag
    21301 aaaatttaaa agataagaga gctgatgaag ttgacagaac tccagcagaa aaccttgctt
    21361 atcaggttgg ttggaccaac ttggttctta aatgggaaga agatgaaaga aaggggcttc
    21421 aagtaaaaac accatcggat aaatttaaat ggaatcaact tggtgaatta tatcagtggt
    21481 tcacagatac ctacgctcat ttatctctgc aagagttgaa agcaaaatta aatgaaaata
    21541 ttaattctat ctctgcaatg attgattcgt tgagtgagga agaattattt gaaccgcata
    21601 tgagaaagtg ggctgatgaa gcgactaaaa cagcgacttg ggaagtgtat aagtttattc
    21661 atgtaaatac ggttgcacct tttggaactt tcagaactaa aatcagaaaa tggaagaaga
    21721 tagtattata aattatattt ttaactttaa aaaatttcat aaaaatggtt accaaaggcg
    21781 atagaagaaa aactatcgtc tttttctttg caaattttta agaagggagg tgatcttgca
    21841 tggactttga atatttttat aacagagaag cggaaagatt taacttctta aaagtaccgg
    21901 agatattagt tgatagagaa gaatttcggg gcttatcagc agaagcaatt atcctttatt
    21961 ccatacttct taaacggaca ggaatgtcat ttaagaataa ctggatagac aaggaaggca
    22021 gagtatttat ctattttact gtcgaagaaa ttatgaaaag aagaaatatc tcaaagccaa
    22081 ctgccataaa aacattagat gagcttgatg taaaaaaaag gaataggact gatcgaaaga
    22141 gtaaggcttg gacttggtaa gccgaacatc atttatgtta aagactttat gagtatattt
    22201 caggtaaaag aaaatgactt acaaaagtca aaaaacttaa cttcagaagt aaaagatttt
    22261 aacctcagaa gtaaagaaaa tgaacttcaa gaggttaaga accttgactc taactatata
    22321 gagaataata agagtaagta tagtaagaga gaatatagtt ttggtgaaaa cggacttgga
    22381 acatttcaaa atgtgttttt agctgttgaa gatatatcgg atttacaaat cataatgaac
    22441 tcacagcttg agaattacat tagacttcct gcaaaactag aatcctagtt catgattgat
    22501 aatacca
//
LOCUS       DBEJAQ010000036        22319 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.036, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000036 DBEJAQ010000000
VERSION     DBEJAQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.036"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..240
                     /locus_tag="ACL297_001471"
     CDS             <1..240
                     /locus_tag="ACL297_001471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071720.1"
                     /translation="NSPCCGWILFSLLILFETLFKPRQLHLAVYVLLTSSVLSTTSKQ
                     CFELTSSVLSATSKQCFEQPAASFLVCSLIFIEYY"
     gene            298..954
                     /locus_tag="ACL297_001472"
     CDS             298..954
                     /locus_tag="ACL297_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HGR0071721.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            944..1267
                     /locus_tag="ACL297_001473"
                     /pseudo
     CDS             944..1267
                     /locus_tag="ACL297_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
     gene            1371..2201
                     /locus_tag="ACL297_001474"
     CDS             1371..2201
                     /locus_tag="ACL297_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0071722.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2355..3209
                     /locus_tag="ACL297_001475"
     CDS             2355..3209
                     /locus_tag="ACL297_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGR0071723.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3309..4682
                     /locus_tag="ACL297_001476"
     CDS             3309..4682
                     /locus_tag="ACL297_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGR0071724.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            4675..5736
                     /locus_tag="ACL297_001477"
     CDS             4675..5736
                     /locus_tag="ACL297_001477"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGR0071725.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5738..6430
                     /locus_tag="ACL297_001478"
     CDS             5738..6430
                     /locus_tag="ACL297_001478"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGR0071726.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6459..7028)
                     /locus_tag="ACL297_001479"
     CDS             complement(6459..7028)
                     /locus_tag="ACL297_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGR0071727.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7163..7777
                     /locus_tag="ACL297_001480"
     CDS             7163..7777
                     /locus_tag="ACL297_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGR0071728.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7755..9425
                     /locus_tag="ACL297_001481"
     CDS             7755..9425
                     /locus_tag="ACL297_001481"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGR0071729.1"
                     /translation="MKNWRQNRMKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSR
                     EKEAQRVAQRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQ
                     LERKASPYISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDF
                     KPAGNSFAIPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIF
                     HIGETVDKESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLT
                     LRQTFANYQKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNI
                     HDIYQLELSQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLR
                     NNISDERETLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVE
                     NYFAHGVDHRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQ
                     ASYSDQRQSIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9437..10723
                     /locus_tag="ACL297_001482"
     CDS             9437..10723
                     /locus_tag="ACL297_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGR0071730.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10775..11308
                     /locus_tag="ACL297_001483"
     CDS             10775..11308
                     /locus_tag="ACL297_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071731.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11386..11856
                     /gene="nrdI"
                     /locus_tag="ACL297_001484"
     CDS             11386..11856
                     /gene="nrdI"
                     /locus_tag="ACL297_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGR0071732.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12149..13411)
                     /locus_tag="ACL297_001485"
     CDS             complement(12149..13411)
                     /locus_tag="ACL297_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGR0071733.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13721..14179)
                     /locus_tag="ACL297_001486"
     CDS             complement(13721..14179)
                     /locus_tag="ACL297_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HGR0071734.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(14176..14616)
                     /locus_tag="ACL297_001487"
     CDS             complement(14176..14616)
                     /locus_tag="ACL297_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGR0071735.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14972..16921
                     /gene="mutL"
                     /locus_tag="ACL297_001488"
     CDS             14972..16921
                     /gene="mutL"
                     /locus_tag="ACL297_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGR0071736.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17246..17713)
                     /gene="ribE"
                     /locus_tag="ACL297_001489"
     CDS             complement(17246..17713)
                     /gene="ribE"
                     /locus_tag="ACL297_001489"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGR0071737.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17714..18919)
                     /locus_tag="ACL297_001490"
     CDS             complement(17714..18919)
                     /locus_tag="ACL297_001490"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGR0071738.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18939..19574)
                     /gene="ribE"
                     /locus_tag="ACL297_001491"
     CDS             complement(18939..19574)
                     /gene="ribE"
                     /locus_tag="ACL297_001491"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGR0071739.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19559..20659)
                     /gene="ribD"
                     /locus_tag="ACL297_001492"
     CDS             complement(19559..20659)
                     /gene="ribD"
                     /locus_tag="ACL297_001492"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGR0071740.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20794..20908)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            21065..21658
                     /gene="ruvA"
                     /locus_tag="ACL297_001493"
     CDS             21065..21658
                     /gene="ruvA"
                     /locus_tag="ACL297_001493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGR0071741.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21668..22231
                     /locus_tag="ACL297_001494"
     CDS             21668..22231
                     /locus_tag="ACL297_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGR0071742.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            22228..>22319
                     /locus_tag="ACL297_001495"
     CDS             22228..>22319
                     /locus_tag="ACL297_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HGR0071743.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLAS"
BASE COUNT         6437 a         4268 c         4842 g         6772 t
ORIGIN      
        1 aatagtccat gctgtggctg gattcttttc agcctactta tactcttcga aactctcttc
       61 aaaccacgtc agcttcacct tgccgtatat gtgttactga cttcgtcagt tctatccaca
      121 acctcaaaac agtgttttga gctgacttca tcagttctat ctgcaacctc aaaacagtgt
      181 tttgagcaac ctgcggctag tttcctagtt tgctctttga ttttcattga gtattattaa
      241 gacaatatga ggagaaagat gaaagaaaaa ggattttagg agggggcgca ggcagccatg
      301 ccaacggctc ttggctatgt cagtatcggc ctggcctgtg gaattatcgg tgcgccctat
      361 gtgacacctg ttgagatggg cttgatgagt ctctttgttt atgctgggag tgcccagttt
      421 gccatgttgg cactgattgt ggttcaagct cctgtggcag ctattgctat gacggttttt
      481 ctaatcaatt tgcgtctctt tttgttgagc ttacacgcat cgacttattt ccgtcatacc
      541 agtctttggc acaatatcgg tatgtctagt atcttgacag atgagaccta tggcgttttg
      601 atgggcgaat tagcccatac agataaggtc aatccgatgt ggatgcacgg gaacaatctc
      661 aacagctatg tggcttggtt tgtgggaaca gtggtcggaa cggctctagg tgacctgttg
      721 ccaaatccag aaatctttgg tttagatttt gccctggttg ggatgtttat cgggattttt
      781 gcttcgcaat tccagatgat gcaaagacgg atttctgtcc gcaatctgct cattatctta
      841 gcagttgttg cagtgtcctt ctttttactt ttgacagtgg tgtctcagtc actagctgtt
      901 ctgtttgcga cgctacttgg ttgtagcatg gggatggttt tagatggtca gtaagtatct
      961 tttattagca gttattttct ctggtcttgt gacctggatt ccccgtatga ttcccttcat
     1021 cttggtcaag tataagggcc tgcctacgat tgttgagcgt tttttgaagt ttttgcctgt
     1081 ttccattatc tttgccttga ttctttcaag agtagtgaca ggtaaggttg ggaaccttcc
     1141 tcaaatcaaa tgactggact tcttagcagt ctttccaaca gcttgggtag cttttcgcta
     1201 ccgcaatcta gtcggtacgg ttctctttgg agtagtcttg attgccgtct tgcgtttggt
     1261 cttttaaata agtcataaag aaaacttatc acagagatag atatcatata atggcgtaat
     1321 tacttttttt ctgataagat tataaggtat tctattttgg aggaaatgac atgaaaaaaa
     1381 tcgttaaata ctcatctctt gcagcccttg ctcttgttgc tgcaggtgtg cttgcggctt
     1441 gctcaggtgg tgctaagaaa gaaggacaag cagctagcaa gaaagaaatc atcgttgcaa
     1501 ccaatggatc accaaggcca tttatctatg aagaaaatgg cgaattgact ggttacgaga
     1561 ttgaagtcgt tcgcgctatc tttaaagatt ctgacaaata tgatgtcaag tttgaaaaga
     1621 cagaatggtc aggtgtcttt gctggtcttg acgctgaccg ttacaatatg gctgtcaaca
     1681 atcttagcta cactaaagaa cgtgcggaga aatacctcta tgccgcacca attgcccaaa
     1741 atcctaatgt ccttgtcgtg aagaaagatg actctagtat caagtctctc gatgatatcg
     1801 gtggaaaatc gacggaagtc gttcaagcca ctacatcagc taagcagtta gaagcataca
     1861 acgctgaaca cacggacaac ccaactatcc ttaactatac taaggcagat ttccaacaaa
     1921 tcatggtacg tttgagcgat ggacaatttg actataagat ttttgataaa atcggtgttg
     1981 aaacagtgat caagaaccaa ggtttggaca acttgaaagt tatcgaactt ccaagcgacc
     2041 aacaaccgta cgtttaccca cttcttgctc agggtcaaga tgagttgaaa tcgtttgtag
     2101 acaaacgcat caaagaactt tataaagatg gaactcttga aaaattgtct aaacaattct
     2161 tcggagacac ttatctaccg gcagaagctg atattaaata atttcttgaa atcatccatt
     2221 ctgagtaaga tgggtggttt ctcagttttt agggatatag ttttaaacta tatatctgac
     2281 tatctaataa caatttgtga aatcaaacaa attgtgagat actagtacgg tattattttt
     2341 aaggagaaag aatcatgaaa atcaaaaaat ggcttggtct agcagccctt gctacagtcg
     2401 caggtttggc tcttgcagct tgcggaaact cagaaaagaa agcagacaat gcaacaacta
     2461 tcaaaatcgc aactgttaac cgtagcggtt ctgaagaaaa acgttgggac aaaatccaag
     2521 aattggttaa aaaagacgga attaccttgg aatttacaga gttcacagac tactcacaac
     2581 caaacaaagc aactgctgat ggcgaagtag atttgaacgc tttccaacac tataacttct
     2641 tgaataactg gaacaaagaa aacggaaaag atcttgtagc gattgcagat acttacatct
     2701 ctccaatccg cctttactca ggtttgaatg gaagtgccaa caagtacact aaagtagaag
     2761 acatcccagc aaacggagaa atcgctgtac caaatgacgc tacaaacgaa agccgtgcgc
     2821 tttacttgct tcaatcagct ggcttgatta aattggatgt ttctggaact gctcttgcaa
     2881 cagttgccaa catcaaagaa aatccaaaga acttgaaaat cactgaattg gacgctagcc
     2941 aaacagctcg ttcattgtca tcagttgacg ctgccgttgt aaacaatacc ttcgttacag
     3001 aagcaaaatt ggactacaag aaagcacttt tcaaagaaca agctgatgaa aactcaaaac
     3061 aatggtacaa catcattgtt gcgaaaaaag attgggaaac atcacctaag gctgatgcta
     3121 tcaagaaagt aatcgcagct taccacacag atgacgtgaa aaaagttatc gaagaatcat
     3181 cagatggttt ggatcaacca gtttggtaat aagaaacagg gaggtgggag agaaaattcc
     3241 acctcttgct tttgtataga gtatagattg taaagaagac tattcgttca tagaaaggta
     3301 gagagaatat ggtttttcct agcgaacaag aacagattga aaaatttgaa aaggatcatg
     3361 tagtccaaca ttattttgag gttttgcgta ccttgatttc taagaaatca gtctttgccc
     3421 agcaggttgg actcaaggaa gtcgcaaatt atctgggtga gattttcaag cgtgttggag
     3481 ctgaagtgga gattgatgag agctatacag cgccctttgt catggcacat ttcaagagtt
     3541 cgcgtccaga tgccaagacc ttgattttct ataaccacta tgacactgtg ccagcggatg
     3601 gggatcaggt ctggacagag gatcctttta cgctttcggt ccgcaatggc ttcatgtatg
     3661 ggcgtggggt tgatgacgac aagggtcata tcacagctcg cttgagtgct ttgagaaaat
     3721 atatgcagca ccatgatgat ttacctgtca atatcagctt tatcatggag ggagcggagg
     3781 aatcggcttc aacagaccta gataagtatt tggaaaagca tgcagacaaa ctccgtgggg
     3841 cggatttgtt ggtctgggaa caagggacca aaaatgcctt ggagcagctg gaaatttctg
     3901 gtggcaataa ggggattgtg acctttgatg ccaaggtaaa aagcgctgat gtggatatcc
     3961 actcgagtta tggtggtgtt gtggaatcag ctccttggta tctcctccaa gccttacagt
     4021 ctcttcgtgc tgcggatggc cgtatcttgg ttgaaggctt gtacgaagaa gtacaagagc
     4081 ccaatgaacg agaaatggcc ttgctagaaa cttatggtca acgaaaccca gaggaagtta
     4141 gtcggattta tggattggag ttgcctctct tacaggagga gcggatggcc tttctaaaac
     4201 gtttcttttt cgagccagcg cttaatatcg aaggaatcca gtctggttat caaggtcagg
     4261 gtgttaagac tattttacct gcagaagcca gtgccaagct agaggttcgt ctggttccgg
     4321 gcctagaacc gcatgatgtt ctggaaaaaa ttcggaaaca gctagacaaa aatggctttg
     4381 ataaggtaga attatactat accttgggag agatgagcta tcgaagcgat atgagcgcac
     4441 cagccattct caatgtgatc gagttggcca agaaattcta tccacagggc gtttcagtct
     4501 tgccgacgac agcggggaca ggacctatgc atacggtctt tgatgcccta gaggtaccaa
     4561 tggttgcatt cggtctagga aatgccaata gccgagacca cggtggagat gaaaatgtgc
     4621 gaatcgctga ttatcacacc catatcgaat tagtagagga gctgattaga agctatgagt
     4681 agagatatta tcaagttaga tcagatcgat gtgacttttc accaaaagaa gagaaccatt
     4741 acagcggtta aggatgtgac cattcacatc caagaagggg atatctacgg aatcgttgga
     4801 tattctggag caggaaaatc aacccttgta cgggtgatta atctcttgca aaaaccatct
     4861 gcagggaaaa ttaccattga cgacgatgtg atttttgacg gcaaggtgac cttgacggca
     4921 gagcagttgc gtcgtaaacg tcaagatatc ggaatgattt tccagcattt taacctgatg
     4981 agccaaaaga cagcagagga gaatgtagcc tttgccctta aacactctga actcagcaag
     5041 gaagaaaaga aggctaaagt agctaagttg ttggacttgg ttggtttggc agatcgtgct
     5101 gaaaactacc cttcacaact atctggaggg caaaaacagc gtgtggcaat tgcgcgtgcc
     5161 ttggccaatg atccaaaaat cttgatttca gacgagtcaa cttctgccct tgatccgaag
     5221 acaaccaagc agattttggc cttgttgcaa gatttgaacc aaaaattagg cttgactgtt
     5281 gtcttgatta cgcatgaaat gcagattgtc aaagacattg ccaaccgtgt ggcggttatg
     5341 caggatgggc atttgattga agagggtagt gtgcttgaaa tcttctcaga ccctaaacaa
     5401 cctttgactc aagactttat ctcaacagct acaggtattg acgaagccat ggtcaaaatc
     5461 gagaagcaag aaatcgtgga acacttgtct gaaaacagtc tcttggtgca actcaagtac
     5521 gctggatctt caacagacga gccacttttg aatgaattgt acaagcatta tcaagtaatg
     5581 gctaatattc tctatgggaa tatcgaaatc ctcgatggta ctcctgttgg agaattggtg
     5641 gtggtcttgt caggtgaaaa agcagcgttg gcaggtgccc aagaagccat tcgtcaagca
     5701 ggcgtacagt taaaagtatt gaagggagga cagtaagatg gaatcattga ttcaaaccta
     5761 tttgccaaat gtctataaga tgggttggtc tggtcaggca ggctggggaa cagctatcta
     5821 cctaaccctc tatatgacag ttctttcctt cattatcgga ggcttcttgg ggctagtggc
     5881 aggtctcttt ctcgtcttga cagcgccagg tggtgtcttg gagaataaag tcgtattctg
     5941 gattttagac aaaattacct caatttttcg tgcgattccc tttatcatcc tcttggcaat
     6001 cttgtcacca ctttctcact tgattgttaa aacaagtatc gggccaaatg cagcccttgt
     6061 cccactttct tttgcagtct ttgccttctt tgcccgtcag gtgcaggttg tcttggctga
     6121 attggatggc ggtgtcattg aggcggctca agcgagcgga gcgactttct gggacatcgt
     6181 gggtgtttac ctatcagaag gtcttccaga tttgatccgt gtgacgactg tgaccttgat
     6241 ttcccttgtt ggggaaacag ctatggccgg tgcggttgga gctggtggta tcggtaacgt
     6301 agccatcgct tatggattta accgctacaa tcacgatgtg accatcttgg caaccattat
     6361 tatcattttg attatctttg caatccaatt cttgggagat ttcttgacta agaaattgag
     6421 tcataaataa aaagagccgc gtggctcttt ttaactgatc agattttctg tgcaaatttt
     6481 ttactcaagg cttgtccaat caaggcaccc actagggctc cgatgacaat acttgcgata
     6541 aatagaagga cagttccagg gtttggagcg accatgatac ggtcgatata ttcttgggat
     6601 tttcctcttg ccagaagagt agccatatag gctttgggcg caatccacat aagcaagatt
     6661 ggtcctgttg tactaaaggc gaaaataatg aaagaaagga agttctttgt tttgtccttg
     6721 tattttccta aatgagctac ttcatctgct aggaggccac agataattcc aggaaggaag
     6781 gaaccagcac cgtgtttagt tcccaagaaa aagagggcaa tgacaaggcc gatagtggta
     6841 atggctccaa agcgcggaac ttttgcgact aggatcatat agacgctacc gccgacaagg
     6901 gcagtaaagg caggcgcata aaacatgttt ccagtttggt caaagagatt gcccaaaagg
     6961 acaccaatcc ccatgcagag gaagtaaaga actgcaaaaa gcagtgtagt taagatagat
     7021 ttcttcatga attgtctcct gataattttt tcacaattct cattgtacca cggtttggtg
     7081 ggattgtaaa tggatactag aagtttttga aaacgcttga aatatgatat aatggtttca
     7141 tagttatttt taggggaata tcatgggtag atttttagac tttgtcttta atcgtttctt
     7201 tttagggatg attgcgacag ccttcttttg gctattaact ttagcaggag ggattatcct
     7261 tggtctagcg ccggctagtg ccaccttgat gagcttatat gcagaacatg gttatagctt
     7321 tcgggaatac agtttgaagg aggcttggtc tctttacaag caaaattttg tctcaagcaa
     7381 cctgattttc tatagctttt taggtgtggg tctagttttg acctatggtt tgtatctctt
     7441 ggtgcaattg cctcatcaga ccattgttca tttgattgcg acccttttga atgtcctagt
     7501 agttgccctg atctttttgg cttatacagt atctttaaaa ttacaagttt attttgcctt
     7561 gtcctatcga aatagtctca aattatcctt gattggcatc tttatgagtc tagcagctgt
     7621 ggccaagatt ctccttggga ctgtgctact tgtagcaatt ggttactata tgcctgccct
     7681 gctatttttt gtaggaattg ggatgtggca tttctttatc agtgatatgt tggaacctgt
     7741 ctatgaaatc atccatgaaa aattggcgac aaaatagaat gaagcagttt tggctacata
     7801 cgcttctaag aacctatagt tcagtgatga tcattatcat tgcgagtttt gcaatcttac
     7861 tctcttacgc tgactgggat tcacgtgaaa aggaagccca gagagtagcc cagcgtgtaa
     7921 ctgctagaac agtgagtgaa attgaatatt accatagaga gtcaacccag atagctcagg
     7981 ctttagttga aaaccaagct cgtattgagg gaatctataa atactttagt cttagcatgc
     8041 cagactattt ttactggcaa ttagagcgga aagcttcgcc ttatatatca gtctctctgt
     8101 atgaaaatgt tgatgacctc tatgttcgaa atgattttgt aactggagtg gccattgctt
     8161 ttcaagatta caaggaagtc tatgtttcta ctaaagacaa acgtagtgga gaaaaaatca
     8221 gggctgagga tttcaaacca gcaggaaata gttttgccat tccggtgtca gatccagtgt
     8281 cagatcaaga tttaggagtg atttacatct ccttggatcc tgctgtttta taccatgcca
     8341 ttgataatac tagaggtcat actccgatgg cagtaacagt gacctcacct tttgatacgg
     8401 agatttttca tattggtgag acagttgata aggagagtga aaattggcta gttggcttaa
     8461 cttctcatgg atatcaggtt caggtggcag ttcctaaaaa ctttgtttta caaggaacag
     8521 tgactagctc tgctttgatt gtgggtttga gccttctctt tattgtcatt ctttatctga
     8581 ctttgaggca gacttttgct aattaccaaa agcaggtagt ggatttagta gaatccattc
     8641 aagtcattgc tcaaggcgaa gaggggcgtc ggattgacat ttccgagaaa gatcaggaat
     8701 tactcctaat cgcggagacg accaatgata tgttggatcg attggaaaag aatatccatg
     8761 atatttacca gttagagctt agtcaaaaag atgccaatat gcgagccttg caggcgcaaa
     8821 tcaatcctca ttttatgtat aatacgctgg agttcttgcg catgtatgca gttatgcaga
     8881 gtcaagatga gttggcagat atcatttatg aattcagtag tctcttgcgt aacaatattt
     8941 ccgacgaaag agagaccctc ctcaaacagg aattagaatt ttgccgtaaa tacagctatc
     9001 tctgcatggt tcgctatccc aagtccattg cctatggttt caagatagat ccagagttag
     9061 agaatatgaa gattcccaag tttaccttgc aaccgctggt agaaaactat ttcgcgcatg
     9121 gtgttgacca caggcggaca gataatgtga ttagcatcaa ggctcttaaa caggatggtt
     9181 ttgtggaaat tttggtggtc gataatggta gaggaatgtc ggctgaaaag ttggcaaata
     9241 tccgagaaaa attaagtcag agatattttg aacaccaagc cagctacagt gatcaaaggc
     9301 agtctatcgg gattgtcaat gtacacgagc gttttgtgct ctattttgga gaccgctatg
     9361 ccattactat agagtctgca gagcaagccg gtgttcagta tcgtattaca attcaagatg
     9421 agtagaaagg gagaaaatgt ataaagtatt attagtagat gatgagtaca tggtgacaga
     9481 aggtctgaag cgtttgattc cctttgataa gtgggatatg gaggttgtcg caacagccag
     9541 tcatgccgat gaagctctag aatatgttca ggaaaatcct gtcgatgtca tcatttccga
     9601 tgtcaatatg ccagacaaaa cagggcttga tatgattcgg gagatgaaag agatcttacc
     9661 agatgctgcc tatatcctgc tctcaggtta tcaggagttt gattatgtaa aaagagcaat
     9721 gaaccttagt gtggtggact atttggtcaa gcctgttgat aaggtagagc tgggaaatct
     9781 gctggagaag attgcaggtc agctcggcga gagagggaag aaaagtcaga ctcttagtca
     9841 agaattagac gaggctggat ttgttagtta tttaggggat aaggagaatt ggtggatagg
     9901 tctatccaag gaaaaacaag gttccttcac cattccctac tatgtcttgg gccaagactg
     9961 gcagattttc atttctggcc aacctctaga tggtttagtc gttacacctt ttgaagctcc
    10021 ttatcaagaa cactttgaac gctggaagct gaatgctgag aaaaccctct tttacggttc
    10081 tgtaaatttg cagcagtctg agagtctctt tgcctattac gaaccgattt atagggttat
    10141 cattcaggga aatctcaatc aaatcgtaga agagttaaat ctcttggaga aggtagttct
    10201 tgaaaatacg ccgcgtgttc cgattactaa acagcttttt attcagtttg tcatggatgt
    10261 tttccattta tttgaacatc tcaaagctga tgacctgaca gacatcgtca aaaccattca
    10321 tgctattcaa tccttcgatg aattggtttc ttatatcaag gaaactctga tcagcttttt
    10381 cggtcaatac cgtatgaatg aaaatgtggt cagtgtgctg gaagtcattg gtcgtgatta
    10441 ccaaaaagag ctttccctca aggatatcag taaggctctc tttatcaatc ctgtttatct
    10501 agggcagttg attaagcgtg aaaccgattc gacttttgca gagttactaa acaaacaacg
    10561 tattaaggct gcccaacaac ttttgctttc aactagtgac agcatcgaag atatttgtta
    10621 tgctgttggt tacagtaacc ttggatattt ctataaagtt ttccgaaaat tgtgcggaaa
    10681 atcgccaaaa gcctaccgaa aacaggtaga aactatacta taagatttgt attcctttac
    10741 aaaaggtgct ataatattat caggaggttc tatcatgaaa aagaaaccaa tttatctatg
    10801 ggtcttgcta atcttgtctg cccttatttc agttccgtct ctgtttggaa ttgtgagtcc
    10861 cttgcctagt aaagaagccc ttcgtgctgc tcagaaacaa gttgcagggg tcaatgctca
    10921 gcaattagaa gaccagctta attataccta tagagtagca gaagcatctc attctatttt
    10981 taatgttgct ttgattgtgc tatctactat tttagtggtc gtagcgattg tcttccttgt
    11041 tcgtaaaaat ttgcaatatg caaactatac ttacgttggc tatgttttgt tagctattat
    11101 tggttcgatt tatggctatg ttggtttaca agacgctgtg cagttggttc aagatgagag
    11161 catgcgtttg acagtgagta ttggttcaaa agctgttagc attttctata tcgtcatcaa
    11221 tgttctcttc ctagcccttg tcttctataa gatgtggcga caacagaaag ccttggcaga
    11281 agaagaggaa acagaagagc ttacctaagt attgacaaaa aagaactatc aagatacaat
    11341 cttggtagtt gttttttgat tatagataaa atcatggagg tacatatgaa gacaatttct
    11401 ttagtttata ttagtttgag tggcaacact gaaagttttg tgacgcgctt gaaagactat
    11461 ctcttgtccc agtacaaagg gattgaggtt caaaagattc atatcaagga tttggtcaag
    11521 gaaggcaaaa atttctatga aatggaccat ccctatgtcg cttttttgcc gacctatctc
    11581 gaaggtggga atggcgtgga taacggagat gttgagattt tgacgacacc ggtgggagat
    11641 tttattgcct atggtaacaa tgctagtaag tgttttggtg tggttgggtc aggaaatcgt
    11701 aactttaata accaatactg cctaacagcc aagcaataca gtcaacgctt tggcttccct
    11761 gtattggctg actttgaaat gcgaggcatg ctggaagata tcaaacatgt cgcagctatt
    11821 atcgcagatt tgtatgagtt ggaaaaggag aattaatact caatgaaaat caaagagcaa
    11881 actaggaagc tagtcgcagg ttgctcaaag cacagctttg aggttgcaga tagagctgac
    11941 gtggtttgaa gagattctcg aatagtataa agttcttata agatgtggtt gaagttgttc
    12001 aactgttttt tgagtataaa cagtctttaa gaacgtaaaa aagcataagt tcaggtcttc
    12061 aaaaccttgt tcttatgctt tttatcatag aaatatttgc tgaattttct cttgaaatga
    12121 aaccttatct tcatctcgcg cttgtccctt accctgcttt tccgattgcg agtgcgtaga
    12181 taaagaagat ggcgaagcca aagaggaaaa tcaatcccgc aatggcaagg tgtttgagcc
    12241 aagaaggaag atttttgttt tcacgtgtta ccaaggcgta attcaaaaga gcaaagaaag
    12301 gtgttgtcag gaaagagcca atcatggcaa agcggagcat ggttgaaacc tgaccagcga
    12361 agaacttgat aatgacgata ccgatgatag cagtgatggt catccagatg ttcaaagatt
    12421 tacgattgtc ctctttttga ctgattagca gtcggagaga ttcctgatta acgcgagaat
    12481 agccatcgat aacagttata actgttccaa agatacagag gaaggcaata aaggtaatca
    12541 agtaacggga ccattcgcca agaacagagg catacatgcc cacgaattga gagatgtatt
    12601 tggctgaagc agcttcaacc gcctgccctg taggatactg aatcagtgct cccagtgcca
    12661 caaagaagac ggctaggata gctgttccaa tataaccagt gttaaagtca aacagagcgt
    12721 cttctgtgtt aaagttgacg gtctttctct tttcagctga ccaaagtgaa ttgatggctg
    12781 aaatttcaat aggagccggc atccatccta ggagggagac gatgaagggc agagctgcca
    12841 tttgccaagg tgtcttctcg acaaaatcag aactgtattc tggatgcttg accgccgcaa
    12901 tgataactgc aagaacagtc gcaatggtta aggcagacat aatccatttg accatgccgt
    12961 ctaaaagttt gtagcctcca aagagtagca tagcccaaat gattgcaacg agaatgaggg
    13021 accactgagt aatgctaagt ccaatcattg ggaaggcact ggcgatgata gctgagcaca
    13081 gaatggcaac accagccgtg ttgaccatag ccgaaaagac attgagaata aagaaaatcc
    13141 agagatagag ttttcctttt tcggcataac cttcaaccaa agtctttcca gtatcagctg
    13201 tgtattcagc accaaaacgg aaaaatggat atttaaagac attggctaag atgaccaaga
    13261 gaagtagaga ccaaccgtaa gaaccgccag cttgagttga ggatacaatg tgggaacctc
    13321 caacagcggc agttgccatt aggattccag gtcccattgc ttttagcttg cttgcccagg
    13381 ttgattgatt taaggaaata gcttgcgaca tgataaatac tcctttttga attttcagaa
    13441 taatctgtat atgcaatcta tattctacaa aaaattttgg aaaaatgcaa gaatattttg
    13501 aaaaaagata gaaaatttac aaagaaaatc tgaaataact gttatgatat agcagattga
    13561 aactagaata gtacactttt gcttctaaaa cattgttaga aatcgatttg actgtcctga
    13621 ttgatttatc ctgttcttat ttcatttcac tatagtaagg tgcagcagga taaagcaaaa
    13681 ccgagaagtt tctttctcgg ttttgcttat tatgaggttc ctattttcta tgctccaaga
    13741 gttgattgat gtgatctact tttttagcct ctcttttata gagaatagcc cagatgatga
    13801 gatagacaat cgcaaactcg ataatgagct ggaggtagaa aatccagtgg aaggggaacc
    13861 aaccagcaag agttgctagt gggacaaagc cagccagcat aaggaagaaa tgagtcagag
    13921 tggcacgaag catgctccag tcacggctga ataagcggtt gccaaagttg aagagcatac
    13981 cgatggctgc ccagataagt gtgcagtaga gcaagaccag ggcaccgtga acctgatgct
    14041 gatccatcac ttggccgatg agagagtagg gatttagtgg tgcgtaggta tttggtgcat
    14101 aaatgagtga aaagaggatg gagaggatga ggccgataag aacaccggca gctgcatcgt
    14161 gaaatacttg ttttttcata gttctaattt ctccttgatg gtttttagat aacggcgtga
    14221 agagtaggtg aagctttcgt ttttcaagaa aatttctacc agaccgtttg gcgtgagctt
    14281 gaggtgagag atggaatcga tattgatgat ttctgattgg gaaatttgga taaaattgct
    14341 tggcaagagt ttaagaacct gatagagtcg caaatcaatg ctgtaggtct gactcgcggt
    14401 ttctgctaga accttccgat tctcgatata gaagcgctga atcttgccaa tctcaactag
    14461 atagacctga tcatcgattt ttcctttgat tttttctctt tggtccagat tttctgcgaa
    14521 ctcgatgact ttctggactt tttcggtttc ttgaggtgct tggacaatca gcttttcctc
    14581 ctcgtaagtc tcactaatct gtagttctac tttcataaat ttctctcctt gtttttcata
    14641 caaaattaat ctcacttctt gtacaccttt attaatactc ttcaaaaatc tcttcaaacc
    14701 acgtcagcgt cgtcttgccg taggatagtt gttgactttg tcagttttat ctacaacctc
    14761 aaaacagtgt tttgagctgt ctgcgtctag ctttctagtt tgctctttga tttttattga
    14821 gtataccact attttactcc ctctggcaag ggactttgtc tatgtggagg gattgggctc
    14881 ctatgtggtg gagtttttct gttctttctg aaatatggta taatagcact aattaatttc
    14941 taggaaaata gatacagaaa ggggctgaaa gatgtctcat attattgaat tgccagagat
    15001 gctggcaaac caaatcgcag ctggggaggt cattgaacgt cctgccagtg tggttaaaga
    15061 gttggtagaa aatgccattg acgcgggctc tagtcagatt atcattgaga ttgaggaagc
    15121 tggtctcaag aaggttcaaa tcacggataa cggtcatgga attgcccacg atgaggtgga
    15181 gttggccctg cgtcgccatg cgaccagtaa gataaaaaat caagcagatc tctttcggat
    15241 tcggacgctt ggttttcgtg gtgaagcctt gccttctatt gcgtctgtta gtgtcttgac
    15301 tctgttaacg gcggtggatg gtgctagtca tggaaccaag ttagtcgcgc gtgggggtga
    15361 agttgaggaa gtcatcccag cgactagtcc tgtgggaacc aaggtttgtg tggaggatct
    15421 ctttttcaac acgcctgccc gtctcaagta tatgaagagc cagcaagcgg agttgtctca
    15481 tatcattgat attgtcaacc gtctgggctt ggcccatcct gagatttctt ttagcttgat
    15541 tagtgatggc aaggaaatga cgcggacagc agggactggt caattgcgcc aagcaatcgc
    15601 agggatttac ggtttggtca gtgccaagaa gatgattgaa attgagaact ctgacctaga
    15661 tttcgaaatt tcaggttttg tgtctttacc tgagttgact cgggctaacc gcaattatat
    15721 cagcctcttc atcaatggcc gttatatcaa gaacttcctg ctcaatcgtg ctattttgga
    15781 tggtttcgga agcaagctta tggttggacg ttttccactg gctgtcattc acatccatat
    15841 cgacccttat ctagcggatg tcaatgtgca tccaactaag caagaggtgc ggatttccaa
    15901 ggaaaaagaa ctgatgaccc tggtttcaga agctattgca aatagtctca aggaacaaac
    15961 cttgattcca gatgccttgg aaaatcttgc caaatcgacc gtgcgcaatc gtgagaaggt
    16021 ggagcaaact attctcccac tcaaagaaaa tacgctctac tatgagaaaa ctgagccgtc
    16081 aagacctagt caaactgaag tagctgatta tcaggtagaa ttgactgatg aagggcagga
    16141 tttgaccctg tttgccaagg aaaccttgga ccgattgacc aagccagcaa aactgcattt
    16201 tgcagagaga aagcctgcta actacgacca gctagaccat ccagagttag atcttgctag
    16261 catcgataag gcttatgaca aactggagcg agaagaagca tccagcttcc cagagttgga
    16321 gtttttcgga caaatgcacg ggacttatct ctttgcccaa gggcgagatg gactttacat
    16381 catagatcag cacgctgctc aggaacgggt caagtacgag gagtaccgtg aaagcattgg
    16441 caatgttgac cagagtcagc agcaactcct agtgccctat atctttgaat ttcctgcgga
    16501 tgatgccctg cgtctcaagg aaagaatgcc tctcttagag gaagtgggcg tctttctagc
    16561 agagtacgga gaaaatcaat ttattctacg tgaacatcct atttggatgg cagaagaaga
    16621 gattgaatca ggcatctatg agatgtgcga catgctcctt ttgaccaagg aagtttctat
    16681 caagaaatac cgagcagagc tggctatcat gatgtcttgc aagcgatcta tcaaggccaa
    16741 tcatcgtatt gatgatcatt cagctagaca actcctctat cagctttctc aatgtgacaa
    16801 tccctataac tgtcctcacg gacgtcctgt tttggtgcat tttaccaagt cggatatgga
    16861 aaagatgttc cgacgtattc aggaaaatca caccagtctc cgtgagttgg ggaaatatta
    16921 aaagtataaa aaagtctggg aaaaattttc aaaatcagaa aaacgcataa aatcaggtgt
    16981 tcaaaaacct tgattttatg cgttttatca tggaaatagt tacttcatgt tttcctaatt
    17041 cttttcgaaa ctctttttaa accacgtcag ttttatcagt aatctcaaaa cagtgttttg
    17101 agctaatttt gccagttttg tctgtaacat cgaagttgtg ttttaccact ctgcgactgg
    17161 tttcctagtt tgctctatga ttttcacaga gtattaaatt gcgattttgc caagtttctt
    17221 tattcgtcta aaagtagagt ctgttctatg cgtctaatgt acgaattagg ttgaccattt
    17281 caatagctcc ttgtgcacac tcagaaccct tatttcctgc tttggtacca gctcgttcta
    17341 tggcttgttc aattgtatct gtcgttagca caccaaacat aacaggaatt tcgctattta
    17401 aactgatttg ggcgattccc ttagatacct cgctacatac ataatcataa tgacttgtat
    17461 tccctctaat gacagctccc aagcagataa ttgcatcata ttttttactt tttgccattt
    17521 ttgatgcaat cagtggtatt tcaaaagctc ctggaaccca ggctacctct atatctttct
    17581 cgtttacatt ctctcttttg agattatcta gtgctccaga taataatttt gaagttataa
    17641 attcattaaa tctcgctaca acaataccta ttttaatatt gtttgctact aaattacctt
    17701 cataagtgtt catttatttt tcctccatat ttaaaatgtg acccattcga tttttctttg
    17761 tttctaaata aaaactatcg taaggattgg cttctatttc gattgatatt ctactggaaa
    17821 tggtaattcc atatttttct aactgttcaa ccttgtcagg attatttgtc agtaaatgaa
    17881 gtgactgaag tcccagatct ttaagcattt gtgctccaat atgatattct cttaaatcac
    17941 cttcaaagcc taatgcaaga ttggcatcaa gcgtatccat gccttgatct tgtaaatgat
    18001 aggcttttaa tttattgata agtccaattc ctcgtccctc ctgtcgcaag taaagtaaga
    18061 cacccgaacc attctcaaca atcattttca tagctttatc gaattgctgt ccacaatcgc
    18121 aacgtaaaga gcctaaaaca tctcctgtta aacattcgga gtggacccga cataatacat
    18181 tggcttcatc ctctatgttt cccataataa gagcaagatg atgttcccca tttagtttat
    18241 ctatatagct aattgctttg aaattaccgt atctagtagg catattgaca gttgaaactc
    18301 gttctatcag ctgatcatat acttttctat attcttgtaa ttctttgatg gtaattagtg
    18361 gaatgttgtg tttcttcgag aactgaatta aatcatctgt tctcatcatt ttgccatcat
    18421 gattcattat ttcacaacat aggccacact cttttagtcc agctaatttt aataaatcaa
    18481 cagttgcttc tgtgtgtcca tttctttcta gaacaccacc tttttttgca attaaaggaa
    18541 acatgtgtcc tggcctgcga aaatcagagg gtgttatatc ttcagctaca cacatacgtg
    18601 cggtcagtcc tctttcctcg gcagaaatac ctgtggtcgt ttctttataa tcaattgaaa
    18661 ctgtaaaagc agtcttatga ttatctgtat tgttttcaac cataggtgaa agcattaatt
    18721 gattagctaa actttcgctc ataggcatac aaattaatcc tttggcataa gtagccataa
    18781 aattaacatt ttctgttgta gctgcttgtg cagaacaaat taagtctcct tcattttctc
    18841 tatccttgtc gtctataaca agaacaagtc gtcccttctg caatgcttct aatgtttctt
    18901 gtatttttcg atattccatt gactgattat cctttctgct aaaatccatt ttgatataat
    18961 agttccttag atatttctga ttttggagag ttatccatca gtttttgcac atatttacct
    19021 aagatatcat tttcaagatt tactgtactc ccgacttgtt tacttttaag aatggtttgt
    19081 ttcaaggtat gagggataac agatactgaa aagtttactt tggagacttt agcgacagtc
    19141 agactaatgc cgtcaattgt aatagatcct ttttcaacta ttaaatctaa aatttctttt
    19201 tgtgtgttga tttgatacca tacagcatta tcatcttttt ttattgacga gatttttcct
    19261 gtaccatcaa tgtgtcctgt aacgacgtga cccccaagtc gaccgttgat agataaggct
    19321 ctttctagat tcacctcact tccatgtttt aatagagtaa gagctgttcg actccatgtt
    19381 tcattcatta catcaactgt aaaggattga tgattgaaat gagtaactgt aagacagata
    19441 ccatttactg ctatactatc gcctaaatgg atatccgtta atatttttga ggctttaatt
    19501 gatagtttac aattacgaga gtctttctgt attctttcaa cttttccgat ttcttcaatt
    19561 attcctgtga acatggataa atcacttcac tttctatgag atagtcattt cctatttgag
    19621 aaaaagcata aggtttcaat ctaatagcgt catttggcaa agaaatgcct tcacctccga
    19681 caggaaactt ggcactgcct ccaaaaattt ttggtgcaat atatattttc agctcatcaa
    19741 caatttgttg ttccaaagca ctccaattca ttagactgcc cccttctaga actaggctat
    19801 caatctgcat gtttcctaga tgttgcatta aactcgataa gtctatatga ttgccttttt
    19861 tctttatgga aagtatttca cagccatgat tttgatatag cttcatttta tttttgtctt
    19921 cagaggaagt ggcaatgtaa gttttaatat catttgctgt ttttacgatt ttagaggtaa
    19981 gaggagttcg taaatgtgta tcgcatatga tacggatagg atttttccct tcctccaatc
    20041 tacatgtcag caaaggatcg tcttgaataa cagtattgac tcccaccata attgcactaa
    20101 catggtgtcg taactgatgc acatgctttc ttgcttcttc ttcagtaatc catttggatt
    20161 gatttgtttt agtggctatt tttccatcca ttgacattgc atatttcata aaaacatagg
    20221 gtacatgctg ggtaatatac tttctaaaac tttttattaa gttaagacac tcattttcta
    20281 aaattccaac agtaacttga agattatttt cctcaagtat ctttactcct tttccagata
    20341 caataggatt acagtctagg cttccaatga ctactcttgt aataccacta tcgattatag
    20401 catctataca gggaggtgtt ttcccgaagt gacaacaggg ttcaagtgtt acataaagcg
    20461 tcgctccgac aggggattct ctacagtttt taagagcatt tctctcagca tgtgggccac
    20521 caaaaaactc atgataacct tgtccgatga tgtgattatc ttttacaata actgcgccga
    20581 ccataggatt gggattgacg taaccagccc ctttttgtgc cagttttatt gctaatttca
    20641 tatattttga atcgctcatc tcactacctc caaaaaaaat ataccttgaa taggggacta
    20701 ctcaaggcat acaaaagaaa acttatgcga ttaacaaaaa tactctgaaa tgacaagtaa
    20761 tcatttcaga gcacgcaaaa agcacaaata tacttttatc ttctttcatc cagactatac
    20821 tgtcggcttt ggaatttcac caaatcatgc ctttcggctc gtgggctata ccaccggtag
    20881 ggaattacac cctgccctga agatagttat tcaattacag atgattatag tacttaattt
    20941 tgaatatgtc aacagataaa taccgattgt ttttgatata ctgtatttgt gataatcgat
    21001 tctcgctcct cggataaaga aaatatgata tactagataa acgaaataag agagaaggaa
    21061 tactatgtac gcatatttaa aaggaatcat taccaaaatt actgccaaat acattgttct
    21121 tgaaaccaat ggtattggtt atatcctgca tgtggccaat ccttatgcct attcaggtca
    21181 ggttaatcag gaggatcaga tttatgtgca tcaggttgtg cgtgaggacg cccatttgct
    21241 ttatggattt cgctcagagg atgagaaaaa gctctttctt agtctgattt cggtctctgg
    21301 gattggtcct gtatcagctc ttgctattat cgctgctgat gacaatgctg gcttggttca
    21361 agccattgaa accaagaaca tcacctactt gaccaagttc cctaaaattg gcaagaaaac
    21421 agcccagcag atggtgctgg acttggaagg caaggtagta gttgcaggag atgaccttcc
    21481 tgccaaggtc gcagtgcaag caagtgctga aaaccaagaa ttggaagaag ctatggaagc
    21541 catgttggct ctgggctaca aggcaacaga gctcaagaaa atcaagaaat tctttgaagg
    21601 aacgacagat acagctgaga actatatcaa gtcggccctt aaaatgttgg tcaaatagga
    21661 gcagagaatg acaaaacgtt gttcgtgggt caagatgaca aacccgctct acatcgccta
    21721 tcatgatgag gagtggggtc agcccctcca tgatgaccaa gcattgtttg agttgttgtg
    21781 tatggaaacc tatcaggcag gcctgtcttg ggaaacggta ctcaacaaac gccaagcttt
    21841 ccgagaagcc tttcatagct atcaaattca ctcagtcgca gagatgactg agactgaatt
    21901 ggaagccatg ctggagaatc cagctatcat tcgaaataga gccaagcttt ttgctacacg
    21961 cgctaacgcc caagtccttc tacagttaca ggcagagtac ggctcttttg atgcctatct
    22021 ttggtctttt gttgagggga aaattgtcgt taacgatgtt cctgattatc gccaagcgcc
    22081 agctaaaaca cccttatctg agaaattagc caaagatctc aaaaaacgag gcttcaagtt
    22141 cacaggccca gtcgccgtat tgtcttttct acaggctgca gggctagttg atgaccacga
    22201 gaatgattgt gagtggaaag gtcttaaatg atgtctaaca aaaataaggg aattctgatt
    22261 tttgcgattc tctatacagt cctctttgtg tttgatggcg ttaaattgct ggcttcttt
//
LOCUS       DBEJAQ010000037        20782 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.037, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000037 DBEJAQ010000000
VERSION     DBEJAQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.037"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(253..1173)
                     /gene="cysK"
                     /locus_tag="ACL297_001496"
     CDS             complement(253..1173)
                     /gene="cysK"
                     /locus_tag="ACL297_001496"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGR0071744.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYNGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1458..2093)
                     /locus_tag="ACL297_001497"
     CDS             complement(1458..2093)
                     /locus_tag="ACL297_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGR0071745.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDNGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2149..3447
                     /locus_tag="ACL297_001498"
     CDS             2149..3447
                     /locus_tag="ACL297_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGR0071746.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSNLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3444..4106
                     /locus_tag="ACL297_001499"
     CDS             3444..4106
                     /locus_tag="ACL297_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGR0071747.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREEGASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4186..4734
                     /gene="raiA"
                     /locus_tag="ACL297_001500"
     CDS             4186..4734
                     /gene="raiA"
                     /locus_tag="ACL297_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGR0071748.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4870..6843
                     /locus_tag="ACL297_001501"
     CDS             4870..6843
                     /locus_tag="ACL297_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGR0071749.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            6840..7292
                     /gene="rplI"
                     /locus_tag="ACL297_001502"
     CDS             6840..7292
                     /gene="rplI"
                     /locus_tag="ACL297_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGR0071750.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7336..8688
                     /gene="dnaB"
                     /locus_tag="ACL297_001503"
     CDS             7336..8688
                     /gene="dnaB"
                     /locus_tag="ACL297_001503"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGR0071751.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8690..8956
                     /locus_tag="ACL297_001504"
     CDS             8690..8956
                     /locus_tag="ACL297_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGR0071752.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9045..10163
                     /gene="cbpD"
                     /locus_tag="ACL297_001505"
     CDS             9045..10163
                     /gene="cbpD"
                     /locus_tag="ACL297_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HGR0071753.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNYQNQSGNISSYGSNNSSTV
                     GWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLKENGLWYYLGSSGAM
                     KTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10246..10348
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10419..10709
                     /locus_tag="ACL297_001506"
     CDS             10419..10709
                     /locus_tag="ACL297_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGR0071754.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10672..11430
                     /locus_tag="ACL297_001507"
     CDS             10672..11430
                     /locus_tag="ACL297_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071755.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11468..12475
                     /locus_tag="ACL297_001508"
     CDS             11468..12475
                     /locus_tag="ACL297_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGR0071756.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12475..13203
                     /locus_tag="ACL297_001509"
     CDS             12475..13203
                     /locus_tag="ACL297_001509"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071757.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13322..13780
                     /locus_tag="ACL297_001510"
     CDS             13322..13780
                     /locus_tag="ACL297_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGR0071758.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            13782..16214
                     /locus_tag="ACL297_001511"
     CDS             13782..16214
                     /locus_tag="ACL297_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGR0071759.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            16535..17188
                     /gene="rr06"
                     /locus_tag="ACL297_001512"
     CDS             16535..17188
                     /gene="rr06"
                     /locus_tag="ACL297_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HGR0071760.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17185..18525
                     /gene="hk06"
                     /locus_tag="ACL297_001513"
     CDS             17185..18525
                     /gene="hk06"
                     /locus_tag="ACL297_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HGR0071761.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSRLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            18608..19135
                     /locus_tag="ACL297_001514"
     CDS             18608..19135
                     /locus_tag="ACL297_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HGR0071762.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19214..>20782
                     /gene="pspC"
                     /locus_tag="ACL297_001515"
     CDS             19214..>20782
                     /gene="pspC"
                     /locus_tag="ACL297_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HGR0071763.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSEN
                     NPTVTSSGQDISKKYADEVKSHLEKILSEIQTNLDRSKHIKTVNLINKLQDIKRTYLY
                     ELNVLEDKSKAELPSKIKAELDAAFEQFKKDTLPTEPGKKVAEAKKKVEEAEKKAKAQ
                     KEEDYRNYPTITYKTLELEIAESDVKVKEAELELVKKEADESRNEGTINQAKAKVESE
                     QAEATRLKKIKTDREKAEEEAKRRADAKEQDESKRRKSRVKRGDLGEPATPDKKENDA
                     KSSDSSVGEETLPSPSLKPGKKVAEAEKKVEEAEKKAKDQKEEDHRNYPTITYKTLEL
                     EIAESDVEVKKAELELVKEEAKGSRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAE
                     EEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAEED
                     YARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQYNG
                     SWYYLNANGSMATGW"
BASE COUNT         6563 a         3427 c         4737 g         6055 t
ORIGIN      
        1 cgctttacca tcatgtccta atcgatttgt tcatatctta tttcaatcca ttataaatag
       61 tgagaaatat ctatcctatc ttctagaatg tcttccaaac gtggaaactc tcgtaaacaa
      121 agaggtttta gaggcttatt taccatggac taaaattgta caagaaaagt gcaaataaga
      181 aatctccaga ttaggaacta tccgtgagtt ctctagtctg gagatttttc aatatacttc
      241 gttattggac ggttacaatt cataaagtgc tgtagagaga taacgttcac cgttatctgg
      301 tgctagggca aggacttttt tacctgtacc taattttttg gcaacctcga tggctccgta
      361 gatagctgca gctgaggaaa tccctacaag gaagccttct tttccaccaa tttcacgtcc
      421 gagtgcaaga gcgtcatctg atgttacacg aacgatacca ttataggctt tagtatcaag
      481 tgtatcagga ataaatccag ctgagatacc ttgaattttg tgaggaccag gtttttcacc
      541 agatagaata gcagattcat ctgcttctac tgcaaaaact tgaatgttag aattttctga
      601 tttgagtgca tgagaaacac cagaaatcgt tccaccggta cctactccag caacaaaggc
      661 atctaatcca tctttaccga aagcagctag tatctcagct cctgttgttc tttcgtgtac
      721 ttctggatta gctggattgt caaattgaag aggaaggaaa ccatcacgtt cagcagcgat
      781 ttcttgagcc ttagcaatag cacctttcat tccctcgcta ccaggagtta ggacgagttc
      841 agcaccataa gcttggataa ttttacgtcg ttctacactc atagtttcag gcataacgat
      901 gacgacttta taccctttag cagcacctac ccatgaaagt ccaataccag tgtttccact
      961 tgttgcttca acaatagtag aaccaggttt cagaatacca tcttgttcag ctttttcaat
     1021 catgctaagg gcaatacggt cttttacaga tgaaccagga ttaaatgctt caagctttat
     1081 atagacgtct gcagcacctt ctggcacgat gttgttaagt ttaacaatcg gtgtttgacc
     1141 gattaattca gtaatgttgt tataaataga catatgaata cctctttgta taagatatct
     1201 ttagtataac gctatccata ccatttgtaa aatatagaaa tcctatcgaa gcgataggat
     1261 ttctatatat catagatcaa agtcattaat tttcaagtga ttgcgttcag ctaattctag
     1321 taaataggtg tgaaggaaaa cgctatcagg tttcgtttga attgcttcat tttctagtat
     1381 attttggtac gcatcttttt attttgctcg ctaaagtagt tgtgagaatg tcaacgaaac
     1441 gctgtattat tcattgttta cactaagttt acaggaactt caacctctcg taaaccttgg
     1501 tcagttaaag tgacttttcc attaaaaaac tccacaagtg cagctttaat agtttctttt
     1561 tcttctttat caacataaat catcgtatcg acttgatctg taaagtttgt atccagctcc
     1621 atgagatcat gttctttaag gaagttactg tactcttggt actgagcata agacatttga
     1681 atagcaatgc cagcctgttc ttttatttca ataataccaa tttctttgac agctaaggcg
     1741 acactgccgg cgtaagcacg aattagtcct ccagcgccta gtttaatacc accaaagtag
     1801 cgtgtcacga ccacacagac attggtgaga ttgtgatttt ctagtacccc aagcatggga
     1861 acaccagcag taccactagg ctcaccatta tcacttgtac gtttaatttc actacgttct
     1921 ccaataataa aggcagagca gttatgtgtc gctttgtagt gttctttttt gatggcagta
     1981 atgaagtcac gagcctcttc ttcgctataa acacgcttgg catggcagat aaagcgagat
     2041 ttttttattt cttcttggac ttggccgtcc tctttaattg tcctaaattc catgatttaa
     2101 ttgtactaaa aaataatcta aagtgctagt ttttacgaat agagaagtat gaaagtaaat
     2161 ttagattatc tcggtcgttt atttactgag aatgaattaa cagaagaaga acgtcagttg
     2221 gcggagaaac ttccagcaat gagaaaggag aaggggaaac ttttctgtca acgttgtaat
     2281 agtactattc tagaagaatg gtatttgccc atcggtgctt actattgtcg agagtgcttg
     2341 ctgatgaagc gagtcagaag tgatcaaact ttatactatt ttccgcagga ggattttccg
     2401 aagcaagatg ttctcaaatg gcgcggccaa ttaactcctt ttcaagagaa ggtgtcagag
     2461 ggactgcttc aagcagtaga caagcaaaag ccaaccttag ttcatgcggt aacaggagct
     2521 ggaaagacag aaatgattta tcaagtagtg gctaaagtga tcaatgcggg tggtgcagtg
     2581 tgtttggcta gtcctcgcat agatgtttgt ttggagctgt acaagcgcct gcaacaggat
     2641 ttttcttgcg ggatagcttt gctacatgga gaatcggaac cttattttcg aacaccacta
     2701 gttgttgcaa caacccatca gttattgaag ttttatcaag cttttgattt gctgatagtg
     2761 gatgaagtag atgcttttcc ttatgttgat aatcccacgc tttaccacgc tgtcaagaat
     2821 agtgtaaagg agaatggatt gagaatcttt ttaacagcga cttcgaccaa tgagttagat
     2881 aaaaaggtcc gtttaggaga actaaaaaga ctgagtttac cgagacggtt tcatggaaat
     2941 ccgttgatta ttccaaaacc aatttggtta tcggatttta atcgctactt agacaagaat
     3001 cgtttgtcac caaagttaaa gtcctatatt gagaagcaga gaaagacagc ttatccgtta
     3061 ctcatttttg cttcagaaat taagaaaggg gagcagttag aagaaatctt acaggagcaa
     3121 tttccaaatg agaaaattgg ctttgtatct tctgtaacag aggatcgatt agagcaagta
     3181 caagcttttc gagatggaga actgacaata cttatcagta cgacaatctt ggagcgtgga
     3241 gttaccttcc cttgtgtgga tgttttcgta gtagaggcca atcatcgttt gtttaccaag
     3301 tctaatttga ttcagattgg tggacgagtt ggacgaagca tggatagacc gacaggagat
     3361 ttgcttttct tccatgatgg gttaaatgct tcaatcaaga aggcgattaa ggaaattcag
     3421 atgatgaata aggaggctgg tctatgaagt gcttgttatg tgggcagact atgaagactg
     3481 ttttaacttt tagtagtctc ttacttctga ggaatgatga ctcttgtctt tgttcagact
     3541 gtgattctac ttttgaaaga attggggaag agaactgtcc aaattgtatg aaaacagagt
     3601 tgtcaacaaa gtgtcaagat tgtcaacttt ggtgtaaaga aggagttgaa gtcagtcata
     3661 gagcgatttt tacttacaat caagctatga aggatttttt cagtcggtat aagtttgatg
     3721 gagacttcct gttaagaaaa gttttcgctt catttttaag tgaggagttg aaaaagtaca
     3781 aagagtatca atttgttgta attcccctaa gtcctgatag atatgctaat agaggattta
     3841 atcaggttga gggcttggta gaggcagcag gctttgagta tctggattta ttagagaaaa
     3901 gagaagaggg agccagttct tctaaaaatc gttcagagcg cttggggaca gaacttcctt
     3961 tctttattaa aagtggagtc actattccta aaaaaatcct acttatagat gatatctata
     4021 ctacaggagc aactataaat cgtgttaaga aactgttgga agaagctggt gctaaggatg
     4081 taaaaacatt ttcccttgta agatgaggaa aaaatttgca aaaatagaat gaaagcgtta
     4141 taataaaatc ataaaaacaa aaatgtttag aaagaaggta ctcatatgat taaatatagt
     4201 atccgtggtg aaaacctaga agtaacagaa gcaattcgtg attatgtagt ttctaaactc
     4261 gaaaagatcg aaaagtactt ccaaccagaa caagagttgg atgcccgaat taacttaaaa
     4321 gtttatcgtg aaaaaacggc taaagtggaa gtaacgattc cgcttggatc tattactctc
     4381 cgcgcagaag atgtatctca agatatgtat ggttcaattg accttgtaac tgataaaatt
     4441 gaacgtcaga ttcgtaaaaa taaaacaaaa atcgagcgta aaaataaaaa taaggtagca
     4501 actggtcaat tatttacaga tgctttggtg gaagattcaa atgttgtcca gtctaaagtt
     4561 gttcgttcaa aacaaattga tttaaaacca atggatttgg aagaagcaat tctacaaatg
     4621 gatttattgg ggcatgattt ctttatctat gtggatgttg aagatcaaac aaccaatgtg
     4681 atttatcgtc gtgaggatgg cgaaattggt ttgttagagg ttaaagaatc ttaatttcaa
     4741 tatgtgtaaa ggtaggttta ctgaattgta aactaccttt ttcttataat tgatagaatt
     4801 actgattaca cttttaaaaa gtcgcttttt gaggggtatt atggtataat gggtgccaag
     4861 aggttttgaa tgaaaaaatt ttatgtaagt ccaatttttc ctattctagt aggattgatt
     4921 gcgtttggag tcttatccac tttcattatt tttgttaata ataatctgtt gacggtttta
     4981 attttgtttc tttttgtagg aggctatgtt tttttattta agaaactgag agtgcattat
     5041 acaaggagtg atgtagaaca gatacagtat gtaaaccacc aagcggaaga aagtttgaca
     5101 gctctattgg aacagatgcc tgtaggtgtt atgaaattga atttatcttc tggagaggtt
     5161 gagtggttta atccctatgc tgaattgatt ttgaccaagg aagatggtga ttttgattta
     5221 gaagctgttc aaacgattat caaggcttca gtaggaaatc cgtctactta tgccaagctt
     5281 ggtgagaagc gttatgctgt tcatatggat gcttcttccg gtgttttgta ttttgtagat
     5341 gtatccaggg aacaagccat aacagatgaa ttggtaacaa gtcgaccagt gattgggatt
     5401 gtctctgtgg ataattatga tgatttggag gatgcaactt ctgagtcaga tattagtcaa
     5461 atcaatagtt ttgtagctaa ttttatatca gagttttcag aaaaatacat gatgttttct
     5521 cgtcgggtaa gtatggatcg attttatcta tttactgact acacggtgct tgagggcttg
     5581 atgaatgata aattttctgt tattgatgct ttcagagaag agtcgaaaca gagacagttg
     5641 cccttgacct taagtatggg attttcttat ggcgatggaa atcatgatga gatagggaaa
     5701 gttgctttgc tcaatttgaa cttggctgaa gtacgtggtg gcgaccaggt ggttgttaag
     5761 gaaaacgacg aaacgaaaaa tccagtttat tttggtggtg ggtctgctgc ttcaatcaag
     5821 cgtacacgga ctcgtacgcg cgctatgatg acagctattt cagataagat tcggagtgta
     5881 gatcaggttt ttgtagtcgg tcacaaaaat ttagacatgg atgctttggg ctctgctgta
     5941 ggtatgcagt tgttcgccag caatgtgatt gaaaatagct atgctcttta tgatgaagaa
     6001 caaatgtctc cagatattga acgagctgtt tcattcatag aaaaagaagg agttacgaag
     6061 ttgttgtctg ttaaggatgc aatggggatg gtgaccaatc gttctttgtt gattcttgta
     6121 gaccattcaa agacagcctt aacattatca aaagaatttt atgatttatt tacccaaacc
     6181 attgttattg accaccatag aagggatcag gattttccag ataatgcggt cattacttat
     6241 atcgaaagtg gtgcaagtag tgccagtgag ttggtaacgg aattgattca gttccagaat
     6301 tctaagaaaa atcgtttgag tcgtatgcaa gcaagtgttt tgatggctgg tatgatgttg
     6361 gatactaaaa atttcacctc gcgagtaact agtcggacat ttgatgttgc tagctatctc
     6421 agaacgcgcg gaagtgatag tattgctatc caggaaatcg ctgcgacaga ttttgaagaa
     6481 tatcgtgagg tcaatgaact tattttacag gggcgtaaat taggttcaga tgtactaata
     6541 gcagaggcta aggacacgaa atgctatgat acagttgtta ttagtaaggc agcagatgcc
     6601 atgttagcca tgtcaggtat tgaagcgagt tttgttcttg cgaagaatac acaaggattt
     6661 atctctatct cagctcgaag tcgtagtaaa ctgaatgtac aacggattat ggaagagtta
     6721 ggcggtggag gccactttaa tttggcagca gctcaaatta aagatgtaac cttgtcagaa
     6781 gcaggtgaaa aactgacaga aattgtatta aatgaaataa aggaaaagga gaaagaagaa
     6841 tgaaagtaat ctttttagca gatgttaaag gaaaaggtaa aaaaggcgaa attaaggaag
     6901 taccaacagg gtatgcgcaa aactttctta tcaaaaagaa tctagccaaa gaagcgactg
     6961 ctcaagctgt aggtgaactt cgtggtaaac aaaaatcgga agaaaaagct cacgctgaga
     7021 tgattgcaga aggaaaagca attaaagcac aacttgaagc agaagaaact gttgtagaat
     7081 ttgttgaaaa agttggtcca gatggtcgta cctttggttc tattaccaat aagaagattg
     7141 cagaagaatt gcaaaagcaa tttggaatta agattgataa acgtcatatt caagtacaag
     7201 ctccgattcg agcggttggt ttgattgatg tgccagtgaa aatctatcaa gatatcacaa
     7261 gtgtaatcaa tcttcgtgtg aaagaaggat aagtttacac cttcttgaca agattgtaaa
     7321 aggaagggaa gtctgatggc agaagtagaa gagttacgag tacaacctca agatatctta
     7381 gctgagcaat ccgttttagg ggctatcttt attgatgaga gtaaacttgt ttttgtgcga
     7441 gaatacattg agtctcggga cttttttaag tatgcccatc gtttgatttt ccaagccatg
     7501 gtcgatttat ccgatcgtgg tgatgccata gatgcaacaa cggttcgtac tatccttgat
     7561 aatcaaggtg atttacagaa tattggtggc ttgtcttact tggttgagat tgttaattct
     7621 gtgccaactt ctgctaatgc ggagtattat gctaagattg ttgcagaaaa agcaatgcta
     7681 cgtcgtttaa ttgccaagtt gacagagtct gtcaaccaag cttacgaagc gtcacaacca
     7741 gctgatgaaa ttattgctca ggcagaaaaa gggctgattg atgtcagtga aaatgcaaat
     7801 cgaagcgggt ttaagaacat tcgagatgtg ttgaatctca actttggaaa tctggaagct
     7861 cgctcgcaac aaacgaccga tattacaggt attgcgacag gttatcgtga tttggatcat
     7921 atgacaacag gacttcatga ggaggagttg attatcttag cagctcgtcc agcagttggt
     7981 aagacagcat ttgccttgaa tatcgctcag aacattggga ctaagttgga caaaacggtt
     8041 gctatttttt cactcgaaat gggtgcggaa agcttggtag atcgtatgtt agctgcagaa
     8101 ggcttggtgg agtcacattc tatccgtaca gggcaattga cagatgagga gtggcaaaaa
     8161 tatactattg ctcagggtaa tctagctaac gccagtatct atatcgatga tacgccaggt
     8221 attcggatta cagagattcg ttctcgttct cgtaaattgg ctcaagaaac tggaaatctt
     8281 ggtttgattt tgatagacta tttacaactt atcacgggaa ctggtcgaga aaatcgtcaa
     8341 caagaagttt ctgaaatttc tcgtcagttg aaaatactag ccaaggaatt gaaggttcca
     8401 gtaatcgctc tgagtcagct ttctcgtggt gtagaacaac gtcaggacaa gagaccggtc
     8461 ttgtctgata ttcgtgaatc tgggtctatt gagcaggacg ctgatatcgt agcttttctc
     8521 tatcgcgatg actactatga acgtggtggt gaagaagagg agggtatccc aaataataag
     8581 gtggaagtta ttatcgagaa aaaccgtagt ggagctcgtg gaacagtgga attgattttc
     8641 caaaaagaat acaataaatt ttcaagtatc tcaaagaggg aggcataaga tgtcagatgc
     8701 atttacagat gtagccaaga tgaaaaaaat caaagaagaa atcaaggcac atgagggaca
     8761 agtcgtagaa atgactttgg agaatggtcg taagcgccaa aaaaatagat tgggtaagct
     8821 aattgaagtt tatccatccc tatttattgt ggagtttggg gatgtggaag gagataaaca
     8881 agttaatgtt tacgttgaat cctttactta ctcagatatt cttacagaaa agaatttgat
     8941 tcattatctt gactaaagtg agaaattttc tcactttttc ttttttctcc gaataattta
     9001 ggtgaaggca atcatcgctt tatattattt ttcaaggagg aagaatgaaa attttaccgt
     9061 ttatagcaag aggaacaagt tattacttga agatgtcagt taaaaagctt gttccttttt
     9121 tagtagtagg attgatgcta gcagctggtg atagtgtcta tgcctattcc ggaggaaatg
     9181 gatcgattgc gcgtggggat gattatcctg cttattataa aaatgggagc caggagattg
     9241 atcagtggcg catgtattct cgtcagtgta cttcttttgt agcctttcgt ttgagtaatg
     9301 tcaatggttt tgaaattccg gcagcttatg gaaatgcgaa tgaatgggga catcgtgctc
     9361 gtcgggaagg ttatcgtgta gataatacac cgacgattgg ttccattact tggtctactg
     9421 caggaactta tggtcatgtt gcctgggtgt caaatgtaat gggagatcag attgagattg
     9481 aggaatataa ctatggttat acagaatcct ataataaacg aattataaaa gcaaacacga
     9541 tgacaggatt tattcatttt aaagatttgg atggtggcag tgttgggaat agtcaatcct
     9601 caacttcaac aggcggaact cattatttta agaccaagtc tgctattaaa actgaacccc
     9661 tagttagtgc aactgtgatt gattactatt atcctggaga gaaggttcat tatgatcaaa
     9721 ttctcgaaaa agacggctac aagtggttga gttatacggc ttataacgga agtcgtcgct
     9781 atatacagct agagggagtg acttcttcac aaaattatca gaatcaatca ggaaatatct
     9841 ctagctatgg atccaataat agttcaactg tcggttggaa gaaaataaat ggtagttggt
     9901 atcatttcaa atcaaatggt tctaaatcaa caggatggct gaaagacggt tctagctggt
     9961 attatttgaa attatctggt gaaatgcaga caggatggtt aaaggaaaat ggtttgtggt
    10021 attatctggg tagttcaggg gcaatgaaaa caggctggta ccaggtctct ggtaagtggt
    10081 attattctta ctcttcaggc gccttagctg ttaatacgac ggtggatggc tacagagtaa
    10141 acagtgatgg agaacgagta tagaaaattg gagtaggaga aatttcctgc tcttttctgt
    10201 aagcagtttc cttgacattt ttcttatttt gcgctatcat atatacatat aatatatggg
    10261 agtctgtgta cagtctgaga ggaagtgtta aacttcgacc gcacctgatc tgggtaatgc
    10321 cagcggaggg aacgatactt agtctaattt tgcacctttt ccatgtatgg taaaggtttt
    10381 tctttttttt aaaaggaaaa cgagaagagg aggttcttat gaaagcaagc attgccttgc
    10441 aagttttacc cctagcacag gggattgatc ggatagctgt tattgatcag gtcattgctt
    10501 atctgcaaac tcaagaagtg acgatggtag tgacaccatt tgaaacggtc ttggaagggg
    10561 agtttgatga gcttatgcgc attctaaaag aagcgctgga agtggcaggg caggaggcag
    10621 acaatgtctt tgccaatgtc aaaataaatg taggagagat tttaagtatt gatgagaaac
    10681 ttgaaaagta tactgagacg acacattagt ctattgggct ttctcggagt attgtcaatc
    10741 tggcagttag caggttttct taaacttctc cccaagttta tcctgccgac acctcttgaa
    10801 attctccagc cctttgttcg tgacagagaa tttctctggc accatagctg ggcgaccttg
    10861 agagtggctt tactggggct gattttggga gttttgattg cctgtcttat ggctgtgctc
    10921 atggatagtt tgacttggct caatgacctg atttacccta tgatggtggt cattcagacc
    10981 attccgacca ttgccatagc tcctatcctg gttttgtggc tgggttatgg gattttgccc
    11041 aagattgtct tgattatctt aacaacaacc tttcccatca tcgttagtat tttggacggt
    11101 tttaggcatt gcgacaagga tatgctgact ttgtttagtc tgatgcgagc aaacccttgg
    11161 caaatcctgt ggcattttaa aatcccggtt agcctgcctt acttttatgc aggtctgagg
    11221 gtcagtgtct cctacgcctt tatcacaact gtggtatctg agtggttggg aggttttgaa
    11281 ggtcttggtg tttatatgat tcagtctaaa aaactgtttc agtatgatac catgtttgcc
    11341 attattattc tggtgtcgat tatcagtctt ttgggtatga agctggtcga tatcagtgaa
    11401 aaatatgtga ttaaatggaa acgtacttaa aatagagcgt ttttgaaaaa gaaaagagga
    11461 aatcaaaatg aagaaaacat ggaaagtgtt tttaacgctt gtaacagctc ttgtagctgt
    11521 tgtgcttgtg gcctgtggtc aaggaactgc ttctaaagac aacaaagagg cagaacttaa
    11581 gaaggttgac tttatcctag actggacacc aaataccaac cacacagggc tttatgttgc
    11641 caaggaaaaa ggttatttca aagaagctgg agtggatgtt gatttgaaat tgccaccaga
    11701 agaaagttct tctgacttgg ttatcaacgg aaaggcacca tttgcagtgt atttccaaga
    11761 ctacatggct aagaaattgg aaaaaggagc aggaatcact gccgttgcag ctattgttga
    11821 acacaataca tcaggaatca tctctcgtaa atctgataat gtaagcagtc caaaagactt
    11881 ggttggtaag aaatatggga catggaatga cccaactgaa cttgctatgt tgaaaacctt
    11941 ggtagaatct caaggtggag actttgagaa ggttgaaaaa gtaccaaata acgactcaaa
    12001 ctcaatcaca ccgattgcca atggcgtctt tgatactgct tggatttact acggttggga
    12061 tggtatcctt gctaaatctc aaggtgtaga tgctaacttc atgtacttga aagactatgt
    12121 caaggagttt gactactatt caccagttat catcgcaaac aacgactatc tgaaagataa
    12181 caaagaagaa gctcgcaaag tcatccaagc catcaaaaaa ggctaccaat atgccatgga
    12241 acatccagaa gaagctgcag atattctcat caagaatgca cctgaactca aggaaaaacg
    12301 tgactttgtc atcgaatctc aaaaatactt gtcaaaagaa tacgcaagcg acaaggaaaa
    12361 atggggtcaa tttgacgcag ctcgctggaa tgctttctac aaatgggata aagaaaatgg
    12421 tatccttaaa gaagacttga cagacaaagg cttcaccaac gaatttgtga aataatgaca
    12481 gaaattagac tagagcacgt cagttatgcc tatggtcagg agaggatttt agaggatatc
    12541 aacctacagg tgacttcagg cgaagtggtt tccatcctag gcccaagtgg tgttggaaag
    12601 accaccctct ttaatctaat cgctgggatt ttagaagttc agtcagggag aattgtcctt
    12661 gatggtgaag aaaatcccaa ggggcgcgtg agttatatgt tgcaaaagga tctgctcttg
    12721 gagcacaaga cggtgcttgg aaatatcatt ctgcccctct tgattcaaaa ggtggataag
    12781 gcagaagcta tttcccgagc ggataaaatt cttgcgacct tccagctgac agctgtaaga
    12841 gacaagtatc ctcatgaact tagcggtggg atgcgccagc gtgtagcctt actccggacc
    12901 tacctttttg ggcacaagct ctttctctta gatgaggcct ttagcgcctt ggatgagatg
    12961 acaaagatgg aactccacgc ttggtatctt gagattcaca agcagttgca gctaacaacc
    13021 ctgatcatca cgcatagtat tgaggaggcc ctcaatctca gcgaccgcat ctatatcttg
    13081 aaaaatcgcc ctgggcagat tgtttcagaa attaaactag attggtctga agatgaggac
    13141 aaggaagtcc aaaagattgc ctacaaacgt caaatcttgg cagaattagg cttagataag
    13201 tagaaaaata gggagttggt gaagattatc ctttaccagc gccctttttc ttttaaaaat
    13261 gagaaaattt cggtataata gtcaaacaag gtcaaggttt aaagagagag gtgggtttgt
    13321 tatgagattt aaaaatacat cggatcatat tgaggcctac atcaaggcga ttttagatca
    13381 atctggtatc gtggagttgc aacggagtca gttggcagat acctttcagg ttgttcctag
    13441 tcagattaac tacgtgatca agacacgctt tacggaaagt agaggctact tggttgaaag
    13501 taagcgtggt ggcggaggct acattcgtat aggacggatt gagttttcta gtcatcatga
    13561 aatgctccgg gagctgcttt actcgattgg tgagcgagtc agtcaagaaa tttatgagga
    13621 tattctccag cttttggttg agcaggaatt gatgaccaag caggagatga atttgctaga
    13681 atcagtagct ttggatcgcg ttttaggaga agaagctcca gttgttcgag caaacatgct
    13741 acgtcagatc atacaagagg tagatagaaa agggaagtaa gatgaactat tcaaaagcat
    13801 tgaatgaatg tatcgaaagt gcctacatgg ttgctggaca ttttggagct cgttatctag
    13861 agtcttggca cttgttgatt gccatgtcta atcacagtta tagtgtagca ggggcaactt
    13921 taaatgatta tccgtatgag atggaccgtt tagaagaggt ggctttggaa ctgactgaaa
    13981 cggactatag ccaggatgaa acctttacgg aattgccgtt ctcccgtcgt ttgcaggttc
    14041 tttttgacga agcagagtat gtagcgtcag tggtccatgc taaggtgcta gggacagagc
    14101 acgtcctcta tgcgattttg catgatggca atgccttggc gactcgtatc ttggagaggg
    14161 ctggattttc ttatgaagac aagaaagatc aggtcaagat tgctgctctt cgtcgaaatt
    14221 tagaagaacg ggcaggctgg actcgtgaag atctcaaggc tttacgccaa cgccatcgta
    14281 cagtagctga caagcaaaat tctatggcca atatgatggg catgccgcag actcctagtg
    14341 gtggtctcga ggactatacg catgatttga cagagcaagc gcgttctggc aagttagaac
    14401 cagtcatcgg tcgggacaag gaaatctcac gtatgattca aatcttgagc cggaagacta
    14461 agaacaaccc tgtcttggtt ggggatgctg gtgtcgggaa aacagctctg gcgcttggtc
    14521 ttgcccagcg tattgctagt ggtgacgtgc ctgcggaaat ggctaagatg cgcgtgttag
    14581 aacttgattt gatgaatgtc gttgcaggga cacgcttccg tggtgacttt gaagaacgca
    14641 tgaataatat catcaaggat attgaagaag atggccaagt catcctcttt atcgatgaac
    14701 tccacaccat catgggttct ggtagcggaa ttgattcgac tctggatgcg gccaatatct
    14761 tgaaaccagc cttggcgcgt ggaactttga gaacggttgg tgccaccact caggaagaat
    14821 atcaaaaaca tatcgaaaaa gatgcggcac tttctcgtcg tttcgctaaa gtgacgattg
    14881 aagaaccaag tgtggcagat agtatgacta ttttacaagg tttgaaggcg acttatgaga
    14941 aacatcaccg tgtacaaatc acagatgaag cggttgaaac agcggttaag atggctcatc
    15001 gttatttaac cagtcgtcac ttgccagact ctgctatcga tctcttggat gaggcggcag
    15061 caacagtgca aaataaatca aagcatgtaa aagcagacga ttctgacttg agtccagctg
    15121 acaaggcctt gatggatggc aagtggaaac aggcagccca gctaatcgca aaagaagagg
    15181 aagtgcctgt ctataaagac ttggtgacag agtctgatat tttgaccacc ttgagtcgct
    15241 tgtcaggtat ccccgttcaa aaactgactc agactgatgc caagaaatac ttaaacttgg
    15301 aagctgaact gcacaaacgt gtcatcggtc aagaccaagc tgtttcaagt attagtcgtg
    15361 ccattcgccg caatcagtca ggaattcgca gtcacaagcg tccgatcggt tcctttatgt
    15421 tcctaggacc cacaggtgtc ggtaagaccg agttggccaa ggctttggca gaagttctct
    15481 ttgatgacga atcagccctt atccgctttg atatgagtga gtatatggag aaattcgcag
    15541 ccagccgtct aaatggagct cctccaggct atgtgggtta cgaagaaggt ggggagttga
    15601 cagagaaggt tcgcaataaa ccctattccg ttctcctctt tgatgaggta gagaaggccc
    15661 atccagatat ctttaatgtt ctcttgcagg ttctggatga cggtgtcttg acagatagca
    15721 agggacgcaa ggtcgatttt tcaaatacca ttatcattat gacatcgaac ttaggtgcga
    15781 ctgcccttcg tgatgataag actgttggtt ttggggctaa ggatattcgt tttgaccagg
    15841 aaaatatgga aaaacgcatg tttgaagaac tgaaaaaagc ttatagaccg gaattcatca
    15901 accgtattga tgagaaggtg gtcttccata gcctatctag tgaccatatg caggaagtgg
    15961 tgaagattat ggtcaagcct ttagtggcaa gtttggctga aaaaggcatt gacttgaaat
    16021 tacaagcttc agctctgaaa ttgttagcaa atcaaggata tgacccagag atgggagctc
    16081 gcccacttcg cagaaccctg caaacagaag tggaggacaa gttggcagaa cttcttctca
    16141 agggagattt agtggcaggc agcacactta agattggtgt caaagcaggc cagttaaaat
    16201 ttgatattgc ataaaagaat aaaagtacca gcatctgacc ataagtcaca gtggagtgaa
    16261 actcaatgaa aatcaaagag caaactaggc agctagccgc aggttgctca aaacactgtt
    16321 ttgaggttgc agatagagct gacgtggttt gaagagattt ttgaagagta taagctgcaa
    16381 gatgaatgat tttcttgtat tgacgttgtt gttgacaaaa agtagcggat aaatgaaatc
    16441 cattccatta tcatagatga taggctggta ggaaattttc aaatagcata cagaaaatag
    16501 atgtatggag ttctggtagt agaaagggag agagatgaac attttagttg cagatgacga
    16561 ggaaatgatt agagaaggaa ttgcagcatt tctgacagaa gagggttatc atgtcattat
    16621 ggctaaggat ggacaagagg tcttggaaaa atttcaagat ctccctatcc atctcatggt
    16681 actggattta atgatgccta ggaagagtgg ttttgaagtg ttaaaagaaa tcaatcaaaa
    16741 gcacgatatt cctgtcatcg tcttgagtgc tctgggagat gaaactactc agtcacaggt
    16801 atttgatctc tatgctgatg atcatgtgac aaaacctttt tctttggtac tgcttgtcaa
    16861 gcgtattaag gcgcttatca gacgttacta cgtcatagag gatctttggc gatatcagga
    16921 tgtaacagtg gattttacct cttacaaagc acattataaa aatgaagaaa ttgatctcaa
    16981 accaaaggaa ttactggtac taaagtgttt gattcagcat aaaaatcaag ttttaagtag
    17041 agagcagata ttggaagaaa tttcaaaaga tgtagctgat ttaccttgtg atagggtcgt
    17101 tgatgtctat attcgtactc ttcgcaaaaa attagcttta gattgtatcg tgactgtgaa
    17161 aaatgttggg tataagatta gcttatgata aaaaatccta aattattaac caagtctttt
    17221 ttaagaagtt ttgcaattct aggtggtgtt ggtctagtca ttcatatagc tatttatttg
    17281 acctttcctt tttattatat tcaactggag ggggaaaagt ttaatgagag cgcaagagtg
    17341 tttacggagt atttaaagac taagacatct gatgaaattc caagcttact ccagtcttat
    17401 tcaaagtcct tgaccatatc tgctcacctt aaaagagata ttgtagataa gcggctccct
    17461 cttgtgcatg acttggatat taaagatgga aagctatcaa attatatcgt gatgttagat
    17521 atgtctgtta gtacagcaga tggtaaacag gtaaccgtgc aatttgttca cggggtggat
    17581 gtctacaaag aagcaaagaa tattttgctt ttgtatctcc catatacatt tttggttaca
    17641 attgcttttt cctttgtttt ttcttatttt tatactaaac gcttgctcaa tcctcttttt
    17701 tacatttcag aagtgactag taaaatgcaa gatttggatg acaatattcg ttttgatgaa
    17761 agtaggaaag atgaagttgg tgaagttggt gaagttggaa aacagattaa tggtatgtat
    17821 gagcacttgt tgaaggttat tcatgagttg gaaagtcgta atgagcaaat tgtaaaattg
    17881 caaaatcaaa aggtttcctt tgtccgtgga gcatcacatg agttgaaaac ccctttagcc
    17941 agtcttagaa ttatcctaga gaatatgcag cataatattg gagattacaa agatcatcca
    18001 aaatatattg caaagagtat aaataagatt gaccagatga gccgcttatt ggaagaagta
    18061 ctggagtctt ctaaattcca agagtggaca gagtgtcgtg agaccttgac tgttaagcca
    18121 gttttagtag atattttatc acgttatcaa gaattagctc attcaatagg tgttacaatt
    18181 gaaaatcaat tgacagatgc taccagggtc gtcatgagtc ttagggcatt ggataaggtt
    18241 ttgacaaacc tgattagtaa tgcaattaaa tattcagata aaaatgggcg cgtaatcata
    18301 tccgagcaag atggctatct ctctatcaaa aatacatgtg cgcctctaag tgatcaagaa
    18361 ctagaacatt tatttgatat attctatcat tctcaaatcg tgacagataa ggatgaaagt
    18421 tctggtttgg gtctttacat tgtgaataat attttagaaa gctatcaaat ggactatagt
    18481 tttctccctt atgaacacgg tatggaattt aagattagct tgtagacaga ttagtttttt
    18541 attaaagttc atatagggtt aacataagtg tgttattctt tgtgtagata aaagaaagga
    18601 tactaatatg gtattagcga ttattttagt aacattcttt attcgattga tttttttaaa
    18661 gcgttcgata gagaatgaga aacgaatcct tagcaatggc gggaaagaat ttggagttga
    18721 gaatacaaaa cgattaacta tggctcatat tgttttttat ctctcttgct tggttgaggc
    18781 aatggtgcac aagacaattt ttgatggcat gggcatggtt ggtttggtct tgcttatttt
    18841 ttctatgctg atgttgatgt tggtgattca cttgttggga gatatttgga cagtgaagct
    18901 tatgcttgtc aataatcaca aatatgtaga tcatatcttg tttaggacag taaaacaccc
    18961 taattacttt ttaaatattt tacctgagtt gattggcttg accttgttga gtcatgctta
    19021 tgtgactttt gttttagttt ttccagttta tgcagttatt ttgtatcgac gaatagctga
    19081 agaggaaaag ctattacatg aagttataat cccaaatgga agcataaaga gataaataca
    19141 aaattcgatt tatatacagt tcatattgaa gtgatatagt aaggttaaag aaaaaatata
    19201 gaaggaaata aacatgtttg catcaaaaag cgaaagaaaa gtacattatt caattcgtaa
    19261 atttagtatt ggagtagcta gtgtagtagt tgctagcttg ttcttaggag gagtagtcca
    19321 tgcagaaggg gttagaagtg agaataaccc cacggttaca tctagtgggc aagatatatc
    19381 gaagaagtat gctgatgaag tcaagtcaca tctagaaaaa atattgagtg agatccaaac
    19441 aaatttagat agaagtaaac atatcaaaac tgtaaatcta attaacaaat tgcaagacat
    19501 taagagaacg tatttgtatg aattaaatgt tttagaagat aagtcgaaag ctgagttgcc
    19561 gtcaaaaata aaagcagagt tagacgcagc ttttgagcag tttaaaaaag atacattacc
    19621 aacagaacca ggaaaaaagg tagcagaagc taagaagaag gttgaagaag ctgagaaaaa
    19681 agccaaggct caaaaagaag aagattaccg taactaccca accattactt acaaaacgct
    19741 tgaacttgaa attgctgagt ccgatgtgaa agttaaagaa gcggagcttg aactagtaaa
    19801 aaaggaagct gacgaatctc gaaacgaggg cacaattaac caagcaaaag cgaaagttga
    19861 gagtgaacaa gctgaggcta caaggttaaa aaaaatcaag acagatcgtg aaaaagcaga
    19921 agaagaagct aaacgaagag cagatgctaa agagcaagat gaatcaaaga ggcgaaagag
    19981 tcgggtaaaa cgaggagatc ttggagagcc agcaacacct gataaaaaag aaaatgatgc
    20041 gaagtcttca gattctagcg taggtgaaga aactcttcca agcccatccc tgaaaccagg
    20101 aaaaaaggta gcagaagctg agaagaaggt tgaagaagct gagaaaaaag ccaaggatca
    20161 aaaagaagaa gatcaccgta actacccaac cattacttac aaaacgcttg aacttgaaat
    20221 tgctgagtcc gatgtggaag ttaaaaaagc ggagcttgaa ctagtaaaag aggaagctaa
    20281 gggatctcga aacgaggaaa aagttaagca agcaaaagcg gaagttgaga gtaaaaaagc
    20341 tgaggctaca aggttagaaa aaatcaagac agatcgtaaa aaagcagaag aagaagctaa
    20401 acgaaaagca gcagaagaag ataaagttaa agaaaaacca gctgaacaac cacaaccagc
    20461 gccggctcct caaccagaaa aaccagctcc agctccaaaa ccagagaatc cagctgaaca
    20521 accaaaagca gaaaaaccag ctgatcaaca agctgaagaa gactatgctc gtagatcaga
    20581 agaagaatat aatcgcttga ctcaacagca accgccaaaa actgaaaaac cagcacaacc
    20641 atctactcca aaaacaggct ggaaacaaga aaacggtatg tggtacttct acaatactga
    20701 tggttcaatg gcgacaggat ggctccaata caatggttca tggtactacc tcaacgctaa
    20761 tggttcaatg gcgacaggat gg
//
LOCUS       DBEJAQ010000038        19264 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.038, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000038 DBEJAQ010000000
VERSION     DBEJAQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.038"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            87..3437
                     /locus_tag="ACL297_001516"
     CDS             87..3437
                     /locus_tag="ACL297_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGR0071764.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3943..4413)
                     /locus_tag="ACL297_001517"
     CDS             complement(3943..4413)
                     /locus_tag="ACL297_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGR0071765.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4430..6703)
                     /locus_tag="ACL297_001518"
     CDS             complement(4430..6703)
                     /locus_tag="ACL297_001518"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HGR0071766.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            7050..10151
                     /locus_tag="ACL297_001519"
     CDS             7050..10151
                     /locus_tag="ACL297_001519"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGR0071767.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVSYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            10234..11241
                     /gene="pfkA"
                     /locus_tag="ACL297_001520"
     CDS             10234..11241
                     /gene="pfkA"
                     /locus_tag="ACL297_001520"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGR0071768.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            11300..12805
                     /gene="pyk"
                     /locus_tag="ACL297_001521"
     CDS             11300..12805
                     /gene="pyk"
                     /locus_tag="ACL297_001521"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGR0071769.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(13550..13765)
                     /locus_tag="ACL297_001522"
     CDS             complement(13550..13765)
                     /locus_tag="ACL297_001522"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGR0071770.1"
                     /translation="MEPKIRNSDYLFIKNTPQIDYNTIGIFQVDGTNYVKKLRQGYLK
                     KTQSRILRHPPRRKQPYSYHRKSCQAI"
     gene            14613..15485
                     /locus_tag="ACL297_001523"
     CDS             14613..15485
                     /locus_tag="ACL297_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGR0071771.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(15631..15975)
                     /locus_tag="ACL297_001524"
                     /pseudo
     CDS             complement(15631..15975)
                     /locus_tag="ACL297_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            16118..16430
                     /locus_tag="ACL297_001525"
                     /pseudo
     CDS             16118..16430
                     /locus_tag="ACL297_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16434..17150
                     /locus_tag="ACL297_001526"
                     /pseudo
     CDS             16434..17150
                     /locus_tag="ACL297_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(17280..18095)
                     /locus_tag="ACL297_001527"
     CDS             complement(17280..18095)
                     /locus_tag="ACL297_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HGR0071772.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(18092..18997)
                     /locus_tag="ACL297_001528"
     CDS             complement(18092..18997)
                     /locus_tag="ACL297_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGR0071773.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(18998..19129)
                     /locus_tag="ACL297_001529"
     CDS             complement(18998..19129)
                     /locus_tag="ACL297_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HGR0071774.1"
                     /translation="MAVKFTKRDDLDKMFEEFAKLPDLKQVTFPDDKEKKVKAEKKN"
BASE COUNT         6001 a         3302 c         4109 g         5852 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtagaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatggcttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cacttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggctgggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgc atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt tcactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttgttttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctctgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gtagatggaa
     3781 gctgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
     3841 catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct
     3901 accaggtttt ttaaaagcat aattgttagt tgtagtcatt tattattctt caaagaaaaa
     3961 tggtggggcg aattttttca gttcttcaaa gcacttttga gcagtatctg catcttcaca
     4021 gatgataaga cagacatcat taccacaaag ggtagcgata gcgtcaggga agctcaaagt
     4081 atcaatgata gaaccaaagg attgagccag tccaggaagg gtttttagta ggacttggtg
     4141 ttgaactggg cgcatccaga caagggcgtc ttccatgtag agttcgagac gtttttccca
     4201 ttttgagatg gaaccattgt taagaacata ataagcgcta tcttcttcgc ggacttttga
     4261 taggttcata tttttgatgt cgcgtgagag ggttgcctgg gttacttgaa tgtcgttctc
     4321 agcaagaagg gcttgcaact cagcctgtgt atgaatcttg ttttttgtga taagagcgcg
     4381 tataagttgg tggcggtgtt ctgatttatt cataataatg taactccttt tagcaaggta
     4441 aggtaagcat ggactgagcg aggtcgacag tcaagtggta gtctgtattg tcacggatgg
     4501 tgatttcaaa gtcagtagta tagaggacta aacggagagt gtctccttct tttagcttgt
     4561 aaatagttgg ctgcagttca aattgaacgt ccatccattc atctgcagta atatcctcta
     4621 ctaacagtaa atcatttcta ttttgtaaat taaggtaacc ttttgtcacg actcgttgtg
     4681 cctctggtct aaatggcaat tcacagagat tttccaacat gtgatagcga ccgttgtcaa
     4741 tggttctagc acttaaaata gctggataag gttgtaggta tttcttttgc ccaaattcta
     4801 gcagttgggc agataagagc cccttgtttg tactggattt gatacgaaga ttgagctgag
     4861 cgcgaccgtt taggtggaga tctttagtca caggaaggtt aatagtaatc tgattggctt
     4921 tcccttgata gagctctgta ttgaaggttt ggtatgtctt accatagcgc tcaaaatcct
     4981 tatctgggta ctggttttga atagcttgct cttcttgacc aagtgagaag gtttcacagt
     5041 tttcttgccc accgaagtta tcaagtgata accaagtctg tggagctgta ttgtcctgcc
     5101 agataacagt aggaagttga aagtctgttt cctgtcctag taatttcttg gtcaataagg
     5161 catttatgga ctcacggaag tcaattgatt gccaattgtt catgtaaaca tgggcaccat
     5221 tatggaaaaa gagatgcttg tgtatatgag taggaagagc atggaacatc tggtaaacat
     5281 gaagtggttt gacattccaa tcctgagaac catgagtaaa gacaacctct gcctttactt
     5341 tatgggcatt gagcagataa ttgcggtcat gccaaaactg attgtagtcc ccagtttttc
     5401 ggtctagctg agctttcact ttttctaagt cagcttggtg agcttcattg ccacggatat
     5461 agtcgccagc taagagatta cgagaatagg ttaactcagc aagggagtca aagtcctcac
     5521 ctggataacc acctgggcta gtcaccagac cgttttcacg gtagtagttg taccatgatg
     5581 aaattcctgc ctcggcaatg ataacttcta aaccatcgac tcctgtagtc gcaagaccat
     5641 tggacatggt acctagatag gaaagtcctg ttgtagcaac ttttccgttt gaccaatcag
     5701 ccttgacttg acgctggcgc gtgtgatcag taaaggcacg gcaacggccg ttaagccaat
     5761 cgatgacatt tttataagcc tcgatttgct ggtagtctcc attagtcatg aaacctgtcg
     5821 agtctttggt accaacacct gagacataga gattggcaaa gcctctcgga aggaagtagt
     5881 cgtttagtgt atagctagag ttgatgtgag ttagcttttc ctcagcctct gctataagct
     5941 cagctttacc ttggggttgg acgagattta gttgaggttt ctctagctca atcttgtgag
     6001 gaagtttaac ctcaagctcg ccctccatct tgtagagagc cttgtcacta gccttgtcat
     6061 tggttccctg atgataaggg ctggctgtca tgatggcagg gatttttcca tcaaaacgag
     6121 ggcgaataat gctaaccttt actaggtctg atagcccttt ttggtcagta tcgacacgag
     6181 actcaacgta aacgacttca cgaatgacat cctggttaga aaaagtagcc aaactcttgc
     6241 cgttaaagta gtggtagtca ttatcctccg gaataagacc atcactaaca agttggtcga
     6301 taagagtatt tccttttttg gtgcgagtat tgagtaactg atagagattt tcaatcaagt
     6361 caccatatat aatgggaaat ccagtttctt tacgaaaaac gtcactatct tcgaagtcaa
     6421 ccaaataaga aaagcctaaa agttgaaaag caacagtata aaaaatatct gctgtcagtt
     6481 catcttctga ttgaaaaaat gtcagcaggt ctgttttttt atcagctgct aggatagaaa
     6541 gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg taaaaaggtt tcaagtgagt
     6601 ctttgtgatt ggctgtattt tgtaaatcaa agccacattt ttttagttca gataagacat
     6661 tttcttttgg aaaattgata taactatatt gattaaaacg catagaacct ccatatagaa
     6721 tgacagttaa ggttattata tcaaaaaaaa agcagaaagg gaattgttaa cttcaaaagg
     6781 aaataatcca ataaaaatga ataaagtact aaattcaata tagagaacag agtaacaata
     6841 agaataaata gatagggtat aaaagttcta ggagatttat attatatgct ttctattttt
     6901 atatacaata tagtataaat ataaaaatga tgacaaaaat acaaatgaat agaaaataaa
     6961 ttagtaagct gatgaaattt ttctcaagag aagccattta taggtgaaaa tggtataata
     7021 tagtgagaag gatagaggag aagtgtaaat tgatcgcaca actagataca aaaacagtct
     7081 atagttttat ggaaagcgtc atttcgatcg aaaagtatgt gagagcagct aaagaatacg
     7141 gctacactca tttggctatg atggatattg acaatcttta tggcgctttc gactttctag
     7201 agattacaaa aaaatacggc attcatcctt tgctagggct tgaaatgaca gtgtttgtag
     7261 atgatcagga agtgaatttg cgctttttag ctctatctag tgtgggctat cagcagttga
     7321 tgaagctttc gacagccaag atgcaggggg agaaaacttg gtcagtcctg tcccagtacc
     7381 tggaggatat cgcggtcatt gtgtcttatt ttgatagagt tgagtcgtta gaactaggct
     7441 gtgattacta tataggggtt tatccagaaa cactagcaag cgaatttcat catcctatct
     7501 tacctcttta tcgggtcaac gcttttgaaa gcagggatag agaagttctt caagttttaa
     7561 cagcgattaa agaaaatcta ccgctcagag aagttccctt gcgttcgaga caagatgtct
     7621 ttatatcagc aagttcttta gagaaactat tccaagagcg ttttccgcaa gctttggaca
     7681 atttagaaaa gcttatttca ggcatttctt acgacttgga tactagtctg aaactgcctc
     7741 gttttaatcc agctagacca gcagtagagg agttgagaga gcgtgctgaa ctggggcttg
     7801 ttcagaaggg gttgactagt aaagaatatc aagatagact agaccaagaa ttgtctgtta
     7861 ttcatgatat gggctttgat gattatttct tggttgtttg ggatttgttg cgttttggac
     7921 gatcgaatgg ctattatatg ggaatgggaa ggggttctgc agtaggcagt ttggtttctt
     7981 atgccttaga catcacgggg attgacccag tagagaaaaa tctgattttt gaacgctttc
     8041 ttaatcgtga acgctatacc atgcctgata ttgatattga tatcccagat atttatcgtc
     8101 cagattttat cagatatgtt ggtaataaat atggtagtaa acatgcggca caaatcgtta
     8161 ctttttcaac ctttggagcc aagcaagctc ttcgagatgt cttgaaacgc tttggtgtgc
     8221 cagagtatga attatctgca attactaaga aaatcagttt tcgtgacaat cttaagtcgg
     8281 cctatgaggg caatctccag tttcgtcagc aaatcaatag taagttagaa taccaaaaag
     8341 cttttgagat tgcttgcaag atagagggct atccaaggca aacctctgtc catgcggctg
     8401 gtgttgtaat tagtgaccaa gatttaacca actacattcc tctaaagtat ggtgatgaaa
     8461 ttccactgac tcagtatgat gctcatggag ttgaggctag cggacttttg aagatggact
     8521 ttctgggact acgaaatttg acctttgtcc agaagatgca agagttgctt gctgaaacag
     8581 aaggtattca tctgaaaatt gaagaaatcg atttagaaga caaagaaacg ttagctttat
     8641 ttgcctctgg taatacaaaa ggtatctttc aatttgagca accaggtgcc attcgtctgc
     8701 ttaagcgtgt gcaaccagtc tgttttgaag atgtcgtcgc gactacttct ctaaatcgac
     8761 caggtgcaag tgactacatc aataattttg tggcaaggaa gcatgggcag gaagaagtga
     8821 ctgttctaga ttcagctctg gaggatattt tggctccaac ttatggcatt atgctctatc
     8881 aggagcaggt tatgcaggtt gctcagcgat ttgctggatt tagtcttggg aaggccgata
     8941 ttttgcgtcg agctatgggt aaaaaggatg cctctgctat gcatgagatg agggcttcct
     9001 ttattcaagg ctccatagaa gcaggccata ctgcggaaaa agcagagcag gtttttgatg
     9061 ttatggagaa gtttgcaggt tatggattta atagatctca tgcctacgcc tactcagcct
     9121 tggccttcca gttggcttat ttcaaaacgc attatccagc cattttttat caggtcatgt
     9181 taaattattc caacagtgat tacttaatag atgcacttga agcaggtttt gaagtagcct
     9241 ctctatccat caatactatt ccctatcacg ataaaattgc caacaagtct atctatatag
     9301 gtttgaaatc gattaagggg ctcagcaagg acttggcgct ttggattatt gaacatagac
     9361 cttattctaa cattgaagat tttatagcta aattacctga gaattatctg aaacttcctc
     9421 tgctagaacc tttggtaaaa gttggtcttt tcgattcatt tgaaaaaaat cgtcaaaaag
     9481 tatttaataa cttagctaat ctatttgaat ttgtgaaaga gttgggaagt ttgtttggag
     9541 atgctattta tagttggcag gaatcggaag attggacgga acaagaaaaa ttttatatgg
     9601 aacaagagct tttagggata ggtgtcagca aacatccact acaagctatt gcaagtaagg
     9661 ctatttaccc gattacccca atcggaaatt tgtcagaaaa tagctatgct attattttgg
     9721 ttgaagttca gaaaataaaa gtgattcgta ccaaaaaggg tgaaaatatg gccttcttac
     9781 aggcagatga tagtaagaaa aaattggatg tcactctctt ttcagactta tatcgtcagg
     9841 ttggacagga aataaaagag ggagccttct actatgtaaa aggaaaaata caatcacgtg
     9901 atggccgtct gcaaatgatt gcacaagaaa taagagaagc agttgctgaa cgcttttgga
     9961 tacaggtgaa aaatcatgaa tcggatcaag aaatttcacg cattttagaa caatttaaag
    10021 gcccaatccc agtcatcatc cggtatgaag aggaacagaa aaccatcgtt tctccccatc
    10081 attttgtagc taaatccaat gaattagagg agaaattgaa tgaaatcgtt atgaaaacga
    10141 tttatcgcta aaaatacgga aaatagaaga attttaaaat caaatgtgat ataatcaaga
    10201 agaatgttaa aagaaaaagg agcataaacc aatatgaaac gtattgctgt tttgactagt
    10261 ggcggagacg cacctggtat gaacgctgcc atccgtgcag ttgttcgtca agcaatttca
    10321 gaaggaatgg aagtgtttgg tatctatgac ggatatgctg gtatggttgc cggtgaaatt
    10381 catcccctag atgcagcttc agtaggggac atcatttctc gtggtggtac ttttcttcac
    10441 tcagctcgtt atccagagtt cgctcaactt gaagggcaac ttaaagggat tgagcaattg
    10501 aaaaaacacg gaattgaagg tgtagttgtt atcggtggtg acggatctta ccacggcgct
    10561 atgcgtttga ctgaacatgg cttcccagct attggtcttc caggtacaat cgataacgat
    10621 atcgttggta ctgactttac aatcggtttt gacacagcgg ttactactgc catggacgct
    10681 atcgataaga ttcgtgatac atcatcaagt caccgtcgta cttttgtaat cgaagttatg
    10741 ggacgtaacg ctggtgatat cgctctttgg gctggtattg caactggtgc tgatgaaatc
    10801 atcatccctg aagcaggctt caagatggaa gatatcgtag caagcatcaa agctggttat
    10861 gaatgtggta aaaaacacaa tatcatcgtc ttagctgaag gtgtgatgtc agcggctgaa
    10921 tttggtcaaa aacttaaaga agctggagat acaagcgacc ttcgtgtaac agaacttgga
    10981 catattcaac gtggtggttc tccaactgcg cgtgaccgtg ttttggcgtc acgtatgggt
    11041 gcacatgctg ttaaacttct taaagaaggt atcggtggtg ttgcggttgg tattcgtaac
    11101 gaaaaaatgg ttgaaaatcc aattcttggt actgcagaag aaggggcatt gtttagcctt
    11161 actgcagaag gtaagattgt ggttaacaac ccgcacaaag ccgatattga gctatctagc
    11221 ttgaataaga gcttgtcata atttacaatt tagttaataa gaccaaaaag gtcatatata
    11281 taaaggagtc acaaaaatca tgaacaaacg tgtaaagatc gttgcaactt tgggtcctgc
    11341 ggtagaaatc cgtggtggta aaaaattcgg tgaggacgga tactggggtg aaaaacttga
    11401 tgttgaagct tcagctaaaa acattgctaa attgattgaa gctggtgcta acacattccg
    11461 attcaacttc tcacacggcg accaccaaga acaaggtgag cgtatggcaa ctgttaaact
    11521 tgcggaaaaa attgcaggta aaaaagttgg tttccttctt gatacaaaag gacctgaaat
    11581 ccgtacagaa ttgttcgaag gtgaagctaa agaatattca tacaaaactg gtgaaaaaat
    11641 tcgtgttgca actaaacaag gaatcaaatc aactcgtgaa gtgattgcgt tgaacgttgc
    11701 tggtgctctt gatatctatg atgatgttga agttggtcgt caagttttgg ttgacgatgg
    11761 taaacttggt cttcgtgtgg ttgctaaaga tgatgcaact cgtgaatttg aagttgaagt
    11821 tgaaaacgat ggtatcatcg ctaaacaaaa aggtgtgaac atccctaaca ctaaaattcc
    11881 tttcccagct cttgctgaac gcgataacga cgatatccgt ttcggtcttg aacaaggtat
    11941 caacttcatc gcaatttcat tcgtacgtac tgcaaaagat gtgaacgaag ttcgtgcaat
    12001 ctgtgaagaa actggaaacg gacatgttca attgttcgct aaaatcgaaa accaacaagg
    12061 tatcgataac ttagatgaaa tcatcgaagc agctgatggt attatgattg ctcgtggtga
    12121 tatgggtatc gaagtaccgt tcgaaatggt tccagtttat caaaaaatga ttatcaagaa
    12181 agtcaatgct gcaggtaaag ttgttatcac tgcaacaaac atgcttgaaa caatgactga
    12241 aaaaccacgt gcaactcgtt cagaagtatc agacgtattc aacgctgtta tcgacggaac
    12301 tgacgctaca atgttgtcag gcgagtctgc aaacggtaaa tacccactcg agtcagtaac
    12361 tacaatggct acaatcgaca agaacgctca agctcttctt aatgaatacg gacgtcttga
    12421 ttcagattca tttgagcgta actctaagac agaagtaatg gcttctgctg ttaaagatgc
    12481 tactagctca atggatatca aattggttgt aactcttact aagacaggtc atactgcacg
    12541 tttgatttct aaataccgtc caaatgctga catcttagca ttgacatttg acgaattgac
    12601 agaacgtggc ttgatgttga actggggtgt tatcccaatg ttgacagatg ctccatcttc
    12661 aactgacgat atgttcgaaa tcgctgaacg taaagcggta gaagcaggtc tcgttgagtc
    12721 aggcgatgat atcgttatcg ttgctggtgt gccagtagga gaagctgttc gcacaaacac
    12781 aatgcgtatc cgcacagtac gttaagaaaa atataaaaac ctatcatatc cagctttcta
    12841 gcctgtatga tgggctcttt ttttgtccag ggggcaaaaa gggggcaaaa ctttcataat
    12901 ggattgatac tcttcgaaaa tcaaattcaa accacgtcag cgtcgcctta ccgtatatat
    12961 gttactgact tcgtcagttc tatctacaac ctcaaagcag tgctttgagc aacctgcggc
    13021 tagcttccta gtttgctctt tgattttcat tgagtatgaa ataagatatg cataaattga
    13081 ttagaaagtc aaatgaattt ctacaaatgt tttagcaatc gcaatctact tgtctagatt
    13141 cgatctgata tattttcgat ttaatgatat ggtatttaaa acctccaaag tagcttgctc
    13201 cattctttta cctacgtgag tctagatgtt atttactgtt ttagcgtttt tgtgttctac
    13261 tctaaccatt atagcattct tctcagctag tgtactaagg agtgtgtgcc tgaaaatatg
    13321 ggaactaagg ggctggttta tcggtttctc tagtttagta tttgcctttt gcaaagtgat
    13381 cttaaatgcc tttctctaaa tttacatatc actattgttt aacaaaatct aatctatttt
    13441 aggtcactta ttcttttttt gaaatgtaga atgaactttt tcaaagtttt tcgaatcttt
    13501 taaaatctgt ttgttttata tcgccattct cccccttttt taattctccc tatatagcct
    13561 gacaactttt ccgatggtac gaatatggtt gctttcgtct aggtggatgt cggagtattc
    13621 gggattgagt ttttttgagg tagccttggc ggagtttctt gacatagtta gtgccgtcta
    13681 cttggaagat gccgatggta ttatagtcaa tctgtggggt attcttgata aataggtagt
    13741 cgctgtttct tatctttggc tccatggact tgctgacgac ataagcgatt gggtcgtagt
    13801 cgtctgggat aatggaaact ccataactaa atcgtcgtcc tgcatcaagc ggctacctgc
    13861 agagataaac tacctaacac gagagtaagt agtctgtctg tagtcgtcca gtctgatgat
    13921 ttttacgata cttcgttttt ctgatcatac agttgcctct cggcataggt cagaacttta
    13981 ccttgtctgg gtggtgcccg ttggtcgtag atagattgga tatcgctagg agaatccttt
    14041 tgaactggag gaaagagggc atcaatcaag ctactgaata ctttaactaa gtcaaatata
    14101 gtatttttct tagtagacct aacccttttt tcataatttc taatggtgtt tttacttata
    14161 cctatcttag tacccaattc ttattgagtc caaccattac tagtctatat tgttttatag
    14221 ttgattgagt ttggaatagt acgctgtagc tgctaaaaca tttctaggaa ttaatttgcc
    14281 tttcctaata gagttgttca tatcttattt caatctacta tatttttcac ctctaacaat
    14341 cgcaatctct tctttatcca tgaacgaaaa cgctttctat ctttgtaagt aaagcatcac
    14401 acgaaatcca cgaaaatgaa aacctttgtt gtgttttcgt aaaaaatttg ttgacagagc
    14461 acgaaacgcc tactataatt aaatcaagtt taaggaaata acaaaaaaac aaatcggagg
    14521 gaaagacgat gaacacatta aatgagaaag taatcaatat ctgtaaagca gtagttaaag
    14581 aaactttaat ccaagacatt taggagaaag aaatgaagaa atcaagaaaa ttagccacat
    14641 taggaatctg ttcagccttg tttttaggct tggctgcttg tcaacaacaa catgctactt
    14701 ctgaggggac gaatcaaagg caaagcagtt cagcgaaagt tccatggaaa gcttcataca
    14761 ccaacctaaa caaccaggta agtacagaag aggtcaaatc tctcttatca gctcacttgg
    14821 atccaaatag tgttgatgca ttttttaatc tcgttaatga ctataatacc attgtcggct
    14881 caactggctt atcaggagat ttcacttcct ttactcacac cgaatacgat gttgagaaaa
    14941 tcagtcatct ctggaatcaa aagaagggcg atttcgttgg gaccaactgc cgtatcaata
    15001 gttattgtct tttgaaaaat tcagtcacca ttccaaagct tgaaaagaat gaccagttgc
    15061 ttttcctaga taatgatgcg attgataaag gaaaggtctt tgattcacaa gataaggaag
    15121 agtttgatat tctattttcg agagttccaa ctgaggcaac tacagatgtc aaggttcacg
    15181 ctgaaaagat ggaaacattc ttctcacaat ttcaattcaa tgaaaaagct cgaatgctgt
    15241 ctgtagtctt gcacgacaat ctggatggcg agtatctgtt tgtaggccac gttggggtct
    15301 tagtacctgc tgatgacggt ttcttatttg tagagaaatt gactttcgaa gagccctacc
    15361 aagcgattaa atttgctagt aaggaagatt gctacaagta tttaggcacc aagtatgcgg
    15421 attatacagg cgagggactg gctaagcctt ttatcatgga taatgataag tgggttaaac
    15481 tttaatagtg ttatgatatg acagtaatct taggattttt atttctattt ttgatgagtt
    15541 ggtgggttat ttcaatcgtt aatagccaga aaccacgcaa aaatgaaaca tttattggct
    15601 atatccaaag atacgatatt gatgggaatg gctatcaatc attaccgtgt cctcagaact
    15661 gagaggagtt cttgaaatcg taaaactact ttgaacaaga gttacttcaa cccattggct
    15721 ccgacggatt aagttgcttt cgtgaacacc aaaatcagcc gcaatttctt cataagtgcg
    15781 gtattctcgc acatattgaa gagtggccat aagaaggtct tctaggctta atttaggttt
    15841 tcgtccccct tttgtgtgtt taagttgata agcagttttt aatacagcta acatctcttc
    15901 aaaagtggtg cgctgaacac caacaagaca tttaaatcgt gcatcagtta gttgcttact
    15961 tgcttcataa ttcatagaac tactatacca tattttgttt cgcaggaagt ctaatgctaa
    16021 aatcttatca gtgagaagat ttcttaaagt ttattctttg tagtttcatc tgtattagta
    16081 cataatttaa cttagagaaa ataggaggac taatcgaatg gaattaaaag attttacaga
    16141 aaaagaacag gaaatgatta agaaaaggct tacaatgtct aatattagtg acaaagaaac
    16201 tactgagaag attctggcgc tagtaccaca agacttgatt aagcgaatcc cgttttttgt
    16261 cagaaaacat gctacaacac gtacgattaa acgcatttca attgaacacc ctgaactcta
    16321 cgctgtagct caaacaagtg gtgagattcc agaaaaaaaa acgcgaagaa ttgcgtcaga
    16381 ttatcacaac tatctttgaa caaaagatga ataagcatag tattaagtag agaatgaaaa
    16441 aatattttat tggcggtttg ggaagcaatg cctatcatag caaggatttt ctttaagaac
    16501 tagattcgca ggtctatttt ctaaatccat atgaaaagca tcttcgagat gaaacagaat
    16561 tgaaatcatg gtttaaaaat gagattgtag aggaagaatc tatctgtctg ataggtcatt
    16621 ctattggagg cgatttagct cgttatttcg catcggaatt tgaagaagta aagaaactga
    16681 ttcttttgga tggtggctat ctagatttag ataagatttt acctttggat acagagttag
    16741 aggaaactaa aaattatatc aaatctcaaa ttatttcgga cttagatgtt cttacttcta
    16801 aagaaaaatc tgaagcaaag cattggtcag aaaatatgga gaaagctgta agacagtcct
    16861 atcactggaa tgttgagtat aatagatatg agttggctat aaattatgaa aatatagaag
    16921 cgatactccg cctacggaga aaaatacaag cttttaagag agaagtggga gataccttgt
    16981 ttattagtcc tcgctatcct aatgaagcta catggagaga ggaagcccta aaagaattgc
    17041 cagactattt tgatactatt tttttagaaa actttggcca tgagctttat actcaagcac
    17101 ctaaagaaat cgctagtcta atgaatgagt ggctcgctta ttttctatga gctacttgta
    17161 ttgctggaaa tggtataaga tgaaaatcta caagtcacta attttcatat aaccaaaaaa
    17221 cgaacaggct ctttttctgt tattcagaag agaacttgtt cgtttttatc ttgattttct
    17281 taaaaagctc tatatacata gggattttct ggtttatcga ctttggtaaa gctgtcgatt
    17341 tccaaggttg gaaggttttg tttgagttct ttatggtagt gattgaccag ttttcctttg
    17401 gctgaaatcc aagtgttgtt ttcatactgc cgggtattgc ccttggtcag caatccatag
    17461 acaccagaat ctgcgataca gtggataatg ccgaatcgga acagaaattg actattggca
    17521 tgactggggt cgttatagac aaagcctgta aactggattg tcttgccctc aaactcatct
    17581 ggatagtcgt agatagcctc catgacttcc atatagtttt cattagtgat ctgaatgctg
    17641 tcttgggata agtatttatc cgccgccgtt cgcatttcct tttcataggc tgattttgaa
    17701 aaataagaac tagtatctgg tttcaaatat tggcttgtag tcccttcact tgtctgaata
    17761 gccagattcg ttccttccga tagagggaaa tgatagcctt tagctgaaac agtctgagaa
    17821 tccaagctaa cagttgggaa agttaagccg atgacaagtg gaatagccag aagagaaata
    17881 ctcgtcatct tggccaatcg gctgttcaga tgactgtggg ttttgacttg cttcatccag
    17941 atatacaatt gaacgatagc caagataaaa gaaagcacca tggagatata ggccagatag
    18001 gaatagtgca tgttgatgta ctggtttagt ttgcccgaca gatggaggta aatagtcagt
    18061 tcaaaatagc cagctaaaac taaaaatcga atcataggat gactccaatc aagagagaat
    18121 agactaagac gacaagagtt acaattgtta tgaagtgact gataaatcgt gctttcaagt
    18181 aatttttcat catgagaata tttttgatat ccagcattgg accaatgacg agaaaggcca
    18241 gaactggtgc caaaccgaaa ctcgagagaa gagaagcacc tataaaggca tccgcctcac
    18301 tacagagcga aagaagaaag gctaaaatca tcaagagcag gatggcaaaa agaggggtcg
    18361 cactgataga ggtcagaatc cgagtcggaa cgtagacctg tattatagaa gcaaagaggc
    18421 agccaaatac caaataacgc cccgtatcaa aaaattcatc aatggcctgc acaaagactt
    18481 gaaaaacttt ttttgcagaa ctcaagtaag aaaaatcatg ctcatgacaa gccagacgat
    18541 tttctttctg aatcggttct tgccagaaaa atcctagaaa aattcctagt attacagcca
    18601 caagaatgga acccagagct cgtaataggg cgacatggaa ggagttgcca aaggcagaat
    18661 aggtcgcaaa aagaacaatg ggattgataa caggtgctgt cacaagaaaa ggaacggccg
    18721 tgtaacttgg aacctttttt tccagaaaac gattgatgat ggggacgatt ccacattcac
    18781 aagaagggaa aagtataccg acaaaggtcc caaaaaagat tctcccccaa cgatttcgag
    18841 ggagaaaatg ataaaccttg tcaggtgtga tataaacttc aatcagccct gagacaatgc
    18901 ttcctatcag aacgaaggga agggcttcaa tgataatgga gagaaaaatg gctccagttt
    18961 gaagtacact agatgggagt tgttgaaaag cagtcatcta gtttttcttt tctgctttga
    19021 cttttttctc tttgtcatca gggaaagtaa cttgtttcaa atcagggagt ttagcaaact
    19081 cttcaaacat cttgtccaag tcgtctcgtt ttgtaaattt gacagccata ggtgtacctc
    19141 gattctaaat tctacctcta ttatactatt attttgcgga aaagggagtt ttttaatttg
    19201 ttttcgatag ttgatactct tcgaaaatct cttcaaacca cgtcagcttc gccttaccgt
    19261 agta
//
LOCUS       DBEJAQ010000039        18971 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.039, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000039 DBEJAQ010000000
VERSION     DBEJAQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.039"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(104..430)
                     /locus_tag="ACL297_001530"
     CDS             complement(104..430)
                     /locus_tag="ACL297_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071775.1"
                     /translation="MILWGFDFANDHAHAFFMDNVEWSHADSYFLSFVSDDVEERYTE
                     NVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDG
                     FDYEEW"
     gene            complement(844..2724)
                     /locus_tag="ACL297_001531"
     CDS             complement(844..2724)
                     /locus_tag="ACL297_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGR0071776.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVMGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2718..3587)
                     /locus_tag="ACL297_001532"
     CDS             complement(2718..3587)
                     /locus_tag="ACL297_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGR0071777.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLYRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3671..6316)
                     /locus_tag="ACL297_001533"
     CDS             complement(3671..6316)
                     /locus_tag="ACL297_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGR0071778.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEVTIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6407..7687)
                     /locus_tag="ACL297_001534"
     CDS             complement(6407..7687)
                     /locus_tag="ACL297_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HGR0071779.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7858..9942
                     /locus_tag="ACL297_001535"
     CDS             7858..9942
                     /locus_tag="ACL297_001535"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HGR0071780.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(9982..11661)
                     /locus_tag="ACL297_001536"
     CDS             complement(9982..11661)
                     /locus_tag="ACL297_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HGR0071781.1"
                     /translation="MKKIKPHRPLPSQTQLAYLGDELAAFIHFGPNTFYNQEWGTGQE
                     DPERFNPTQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFDCRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12417..13646
                     /gene="arcA"
                     /locus_tag="ACL297_001537"
     CDS             12417..13646
                     /gene="arcA"
                     /locus_tag="ACL297_001537"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HGR0071782.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13704..14720
                     /gene="argF"
                     /locus_tag="ACL297_001538"
     CDS             13704..14720
                     /gene="argF"
                     /locus_tag="ACL297_001538"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGR0071783.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14885..15832
                     /gene="arcC"
                     /locus_tag="ACL297_001539"
     CDS             14885..15832
                     /gene="arcC"
                     /locus_tag="ACL297_001539"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGR0071784.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVTENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            16041..17552
                     /locus_tag="ACL297_001540"
     CDS             16041..17552
                     /locus_tag="ACL297_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGR0071785.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17574..18905
                     /locus_tag="ACL297_001541"
     CDS             17574..18905
                     /locus_tag="ACL297_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGR0071786.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         5312 a         4310 c         3907 g         5442 t
ORIGIN      
        1 tgctggtatt atcaatcatg aattaggatt ctagttttgc aggaagtcta ttgaaatact
       61 aagcctacac agactgattc caattgtttt ttagatttca attttaccat tcttcatagt
      121 caaaaccatc ataatctgga tattgttctg gcgacgttcc aaccgaacgt acgagataag
      181 cttcttcgtc ctctgtctcg atttctctca gtacttggca ttcaaaacgc cattcatcac
      241 cgaagtcgaa aataaactta aatttttgtt tgacacttag gctatccaga tagacatttt
      301 ctgtgtaacg ttcttcaaca tcgtcactaa caaagctaag aaagtaagaa tctgcatgac
      361 tccactcaac attatccatg aaaaatgcgt gagcatgatc atttgcaaaa tcaaaacccc
      421 ataaaatcac atctgctagt tccgctaacg tttttgtttt tggaatctat atatgtcggt
      481 agcaaccttt tttaaatttg acggaaatga tccaagcttc tacagatgct ggcttttcag
      541 cttctatttc ttgttttact aattttaagt gtagcataat acctccaatt gctcatcgat
      601 agaattgctc ttaggaaaca ttcactttct gctattttat cataagtttg tctaactgta
      661 gcatttgaaa ccagataatt cttctgtttc acgcgaaaca ccctgcgcca agcaaggtgt
      721 tcttgttttt atactcaatg aaaatcaaag agcaaactag gaagctagct gcaggctgct
      781 caaaacactg ttttgaggtt gtagatggaa gctgacgtgg tttgaagaga ttttcgaaga
      841 gtattaagtc gtataaatcg tcgaagctgt cgcaatggta tgccactggt tagctgtagt
      901 tgctgcgaaa tccttgtctg cgtagaagtc agtaaatggt aggatttcaa cttccagctc
      961 gtcgatgcgg tcaagctggc cagccagata aacctcgata cggctttctg ctcgtttgat
     1021 gcgttgcaag agtccgccca tacggatgtc aactgtatcc aaaccaaaga ccttgttttc
     1081 tttcaaccat tggtgactaa agagggcatg gaagtcttca atttggcttc taagttctgg
     1141 taattcttgt ctggcgattt gttgtaaact ttctttatca tccgcttggt aggcctgacg
     1201 aatgcgtcgt cccacatcta ctttgctact taaaatagca ttcaactggg cctgagtttc
     1261 aaagagataa gcatagtttc cagctttttc tttaatgtca gcaatagttc ccgccgcctg
     1321 agcgaagtgt ggcttgtcct gttcaggtgt catgtgtcgg tcaagtatcg gacagagaac
     1381 atcctgataa aagacatagc ggttgggatt gatgccactg agattacctg gtaggtctgg
     1441 taagaggttg gcaaggtcaa tctgcataaa atcctcaacc gttagaccag tattggtctt
     1501 gaaatgcgca gacaaactat ctaggtcatt gcgatagctg agttctgccc agatttgcaa
     1561 gcttggtagg atagagaact gggcagtttc accaccattg tctccccaac ccattacgat
     1621 gacttcttta atctgattgg cacggcaggc tttattagcc tcgatagcca ctagacggct
     1681 aaaatggttg tgaggtgtaa agccaatcca cttccaagct ccccctgcaa atgcaaggtc
     1741 atggctaatc ttgtgatgat tgcggaaatt acggttgtat ttttcctcgc tatcctgata
     1801 ataatcccag taaaccagag tcacacggtc tttgagacgg tctaggtaga cacgagtttc
     1861 ctctggaatt tccacatcac gatcgtactg gccatccgct gacatgagtt tgaagaacat
     1921 atcactccac atctggcagt ggaaaccata tttgtcagca atatccagca cgcgctccaa
     1981 gtgttggcac atgaggagac tacgatccac aacaccgttc aaaatcaggt agcgtcccaa
     2041 accaaccaag tgggcttcgt ccatcccgat attgaccttg cgagtcttca gtttagacaa
     2101 cgtggcaaac atgccatcaa tcaggtcata aaccttttct tcgccaatga gaagaatgtc
     2161 ctctacatca cggagctcct gcacttcctt gacaccccat ttgacaaagg ccgacaagtg
     2221 ggccaaggtc tggatgcatg gtacaaaggt catgtcaaac tgttgagcat aggcttcgat
     2281 ttcctgcaac tcctctgctg aataagctcc acggaaatag ccaaagtaag gctgtccttc
     2341 aatctggtaa gtgtcttcca tgtaaagctc aaaggttgag tagcccatga gagccaagac
     2401 ctcaatcatc tgcttggcag aagccacatt cagcaccgca tttcgagaac agtcaaccat
     2461 gtaagccaaa tcttcgtaag ctgcttgttc ctcaatctct actttatcag cttctgctag
     2521 aactgttacc aacaaggata aggcacgata aagttggtga ggtttgcggt aggtcaattg
     2581 atagtgacct tcctcaccct cgatagagat agaggcttgg tcagactgag tgacagccac
     2641 ttccacatct ggtagagaaa tgtgaccttt taaaacctca atagcttgcg tttgtttggg
     2701 actaagtcct gtaaatctta ccattgcttt tcctcctgta gccagttgac aagggcaccg
     2761 tagagattgg catctgcgtg ataggtgcag gcctggataa ctggtgcgac cgtgtattct
     2821 tcgtaggcat cgacaaagtc ttcaacagcc ttcttaacac cttggataaa atctggattt
     2881 tgactgatag agccacccag actgatgaca tctggatcga tcagatactg gatattgagc
     2941 aagccttgcg tcagattgcg gttcatgcgc tcaatggctt cttgacaaag agcattacca
     3001 gctgcggcct cttggtaaat cttgcgaccg tcccaatcag tatgaccaga tttttcaatc
     3061 acgtatcgta ccatattccc agttgatgct agttgcgacc agttattaag tttttcagca
     3121 ggggcaaggg ttgtcatgta gccaaattct ccacccagac cgtggcgacc tcgataaagt
     3181 ctaccattga taatcatggc tccgccaatc cctgtcccaa tcacgacaca ggctgcattt
     3241 tcaagctctg gatgagctag tagttcactg agtccaacgc agttggcatc attttctaaa
     3301 tggacaggta gctgataaga gctaagcgcc tcataccaag aaaagccatg gatgtagggc
     3361 accgcactga agccatcaat cacacctgtc tcttgattga ccgcacctgg aacgctcata
     3421 gcaatcccac tgtaatcctg ttctgacaag cgttgatcta gccacgctag taaatcctcc
     3481 aagttttcag gcgttgaaat acttgtctta tccagtattt tcccatcagg agtcagactg
     3541 gcaaacttaa tcccagtccc tccgatatca atcgttgcaa tggtcattgt ttttaccata
     3601 aaaaggggcg aatcagcagt tagttcttac tttttcgccc ctcctttctt ttgatgttta
     3661 ctttttgaaa ttaaatttct tcttttttga tgaattctgt acgaatctct tgtggagcca
     3721 atagtcctga atgaactgga tatggtcgct caagtaagtc aagaatggtt tgttgatgtt
     3781 ctgatatgcg cacattttct tgactcatat tatagtaacg gaggacatag ccttcttcat
     3841 tttccgccac tttaaaggct gttggacaaa cttgcggtat gctgagaaca gaatggctca
     3901 agaggctacc agtcgcaacc acgcttcctt cctgtctagc aagctgaagg ctggtaaacg
     3961 gtgtctgcaa ggctttggca cgacgatagg ctgagaagcg ttcttgggct tggtggcatt
     4021 caatcgcaaa ttcgacttca aactcacgca agcattgtgc ttctggcgtt gggaagtagc
     4081 cccagtcacc tagctcacct gatgcacgca aaatggtcac ggcaatggtg ttatccccaa
     4141 ggatttcgta ttcattcaat cccttgttgg atacagtcac ccctttttca tcgtcataca
     4201 gactgacaaa agcttgttgg tgttgaggat tttcaggatt ttcccatgaa gcagctggtt
     4261 tgtttggtcg tgtcaccacc tcatagatac tttcagaatc attgcttgga cgcgtgttat
     4321 gagtcttgac caagagacgg atacggtgat ccttggcagt gttagtaaag cgagtcttga
     4381 agcggatttg tggattgtca acaaagacaa tcaactcagt ttccagagga atgtttgtca
     4441 attcttctga ccgtccagcc tcacgcttca taaactcgat gataccttgt tgctcttctt
     4501 ctagcttttc atccgcactg acaggcacgg tcaattcatg tttgagcaag attttagcat
     4561 agcgatctgt gttttccaag acctcgtggc ccttaagctc tgcaaagatt ggctctgttc
     4621 cttttggttg gaaatagata tactcgtttc cgatgtcacc ccggtcttcg aagcggataa
     4681 agtcttcata ggcttcgtga gttgtcttgt catagactgt gatgttgtca tccacactca
     4741 ccgttacgaa tggtgtatca atcactccgt tttggtaaat accctcacgg tgttcttgtt
     4801 ctccttccag caattggaag gttgtccaag aaagcggtgc taggtgaact ggaatggtca
     4861 cgcgcacttg tcgagcaata cgagcttggc ggaacttatc ttttggtaaa tcgtattcaa
     4921 aattagctcc gaggtcttcg attgtaacct ctacaggacg accatccaag tcctccacac
     4981 ggtaacttgg caagataaga gcagccatct ttttgtagcc ttctgttggg tgcaattcct
     5041 tgaaatcaca agtcgccaca tcaatcactg tgctgacagt atcgacctta tcatgcaagc
     5101 ctgtgttaat gacagtaaag agatagtcac tttgagcctt atccgtagca attttaccct
     5161 tccactcgtt gagcaaatta cttttaacaa agtttcctac ttggttgacc ttggcaaaac
     5221 gcgtttccat ctcgcggtga acttcgtcca cgctacagcc acagatacta tcatgtggcg
     5281 cattctgcaa aagtgttttc caagcatagg tcaactggtc cttgtggttg tgtccaccag
     5341 tgataatagt caagggttct accacttgct ctaggaggtt gctattttct tggaaggctt
     5401 gttttaggta aatgcgggat gaagaagtgt tggcaagtgt gtaccagcca tctgtttcct
     5461 gactggtcaa ctcgcctgta acagttgata agtgttcagg aagcgcacct tctacagctt
     5521 gaacatattc atcaaaagaa ctatgaacaa agattacatc cgggaagagt tcatttgcca
     5581 cacgaatggc ttcgctcaga tttttctgta caggctggtg gtcacagccg ttcatcatca
     5641 accattggtt ggtcgaagcg taggcacgca catctgacaa tttttgtttc cagaaggtca
     5701 aggcctcatc tttgtcaact gggatttcat tcccattact gtaccagttg gcaaagagaa
     5761 tacctaaaac acgactacca tccacaccct gccagtacat ttctgaaaac tgggatgtaa
     5821 accgctcatc ttcaaggact tggttgtcaa atccaatcgg cttcacacca cgaccaaagg
     5881 ctgccacgtg aatacctgat ttttgaagga tttgaggagc ttgtcccata tttccaaagg
     5941 tatctggaaa gtagccaatc tgggttgatt taccccattt ggcagcttct tgttgaccaa
     6001 tcaaggtatt gcggacattg gcttcactgg agatcaagta gtcatcctgc aagatgtaaa
     6061 agggaccaat tttaagtttg ccctcgtcaa tgtagcgttg gaccttgtcg cgattttcag
     6121 ggcgaatttg taagtagtca tcaaggacaa tagtttgtcc atccaagtgg aaactcttga
     6181 actcagggtc attttcaaag agatcaaaga gattgtcaaa caattccacc aactgcatac
     6241 gatggctttc aaaaggcaag taccactcac gatcccagtg actatgtgag ataatatgta
     6301 caacaacatt ttccatgaag taaaacctca ttctaaattt aaattttaac gttttaaatg
     6361 taaacttgtg attctgataa gaatcggttg agctaaagcg agccccttag cggatatcca
     6421 agtaatccaa gaccaactca cagaacatca tgttagccca ggagaaccat tcacgagagt
     6481 agagggtcgg atcatctacg tggaagcttt cgtgcatgac acctgtacca ccatcgcagg
     6541 caaccagctg atccagcaag aattttttct ctgccttatc tcttgttgtc aatccttgga
     6601 tagaaagggc gattggccag atatagcgat agaaggtatg agaacttccg agaccgctag
     6661 cgtattctcc ttggtagaag tatggatttt cagagctcag aatggtacgg cgagtggctt
     6721 gatacacttc atcatcgatc gaacagtagc ccagataggg cgcagctagt agacttggta
     6781 catttggatc atccatgatg ctggcatttc ctaggccatc tacttcaaaa gcgtaaatct
     6841 tttcgccctt gctgttggtg gtgtaagcgt agttttcgat tccttcttgg atttcatcct
     6901 gaagacgctt ggcatcagca ataacactct ggctatcagc taggtttaat gctgcgaaga
     6961 tttcttgcac ataacccaag actactacag caaacatatt tgacggaatc aagtaactat
     7021 actggcaaca gtcatcactc ggacgaaagg ctgaccaagt catacctgtc actgcaaagt
     7081 caggtccaaa gccatcattt accaaggtgt cttccttacg gtccgtatct cggacaaaac
     7141 gataaggaga gttcttgtgg tcttgttcaa ctgtccatag atggagaatt tccttagtcg
     7201 ctgcgacaaa agtttcatca aactgactag tctcgccagt ctctttccag aggagataag
     7261 ccaactgcaa aggatagcaa agcgaatcca cctcatactt gcgctcccag atccagccgt
     7321 taaggtctgt gtggtcagtc tcgtggtgcc ctttccagtt ctcctcaatg ttgaaggagt
     7381 tagcataggg atccttgagt accaaggtca tctgacgttt gaccaaacct gcaatggtct
     7441 gacgcaggag ggcatctctt ttagctacat gaaggtaggg tctgagttgg gctgtcgaat
     7501 ctcgaagcca catggcagga atatccccag tcaagacaaa agttgaacca tcttctaaga
     7561 tttcaaccgt attgtccaag gtatcggtgt agcaacgctc gaaaacatcc acccactctg
     7621 gatggtcctt agcccgctct gctacttcat ctagccattc tctaacaatt tctttcgaat
     7681 aaatcatcgt gcaatttcct ctttcaaaca agtttcattt tcagtatata gcttttgaga
     7741 cagaaaatac atacacaaat gatagtcatt tttctacaaa atagttattt ttgaaactcc
     7801 ttttctaaag gccctctttt tctgatataa tgtagaaaaa cagctaaagg aaagactatg
     7861 aaaccactac ttgaaaccat cgatacccgc tttggtactg ctagcaaaca tgccttctct
     7921 cggggaaata ccctgccata cacaggcgtg ccttttggga tgaattattt tgtgccccag
     7981 accagtgacc aggatggatc ttggtttttt gatccgcatc tgcctatctt tcaggggatt
     8041 cgattaactc accagcccag tccttggatt ggggactact cttggctcct tctgacacct
     8101 gtcaccagtc aactgggggg agacagcctc tttcaccgcc agtcttctta tgatatagat
     8161 aaagcttgct tccagcctca ctatttgaag ctcttttccc tgcgctatca aattgaaacc
     8221 cagctcacac cgacttgcta tggtgcttct attcgtttga agcaaaagca aggcaaagcc
     8281 ctctccctct atcttcacgc agcagatgaa ctgaccgtag agcaagtaga taagcggact
     8341 cttgccctgc gacaagaagg taaaactgaa actaacaaaa attcgctaac gatgttcact
     8401 gccctgcaaa tgaacacgga tattcttgct atcagccaag aagctgggga ctggcgaatt
     8461 gacttagcaa gtagtcaaac cgagatgcaa ctagccactt ctttcatctc tccttctcaa
     8521 gctctaatca atctacctca agaagatttt gatagctgta aaacaagtgc ccaagcggat
     8581 tgggaaaatc tcctccatcg ttttgacatt atagagacag gagaggctga ccgaaccttc
     8641 tttgaccact gcctctacag actcttcctc ttcccacaga ctttttatga ggttgatgag
     8701 tcagggaaag ccatccacat ggatctggct actggtactg tcaagcctgg tgtcctcttt
     8761 agcaacaatg gtttctggga taccttccgc accaccttcc ccctctttgc ccttatcata
     8821 ccggagcact atcaacgctt tttagaaggt ttcctcaata gctaccgcga tactggtttc
     8881 cttccaaaat ggctggctcc agatgaacgg ggtatgatgc caggtacact gctagatggc
     8941 attatcgcag atagcgcctg taaggacatg gcccccgacc tagaaggaga actcttccaa
     9001 gccatgctcg aaacagccag caaagccgac cctctcggca tcaatggccg ccacggacta
     9061 gcccaatacc aagaactagg ttacctctct accgaccacc acgaaagtgt tagccatact
     9121 ctagactatg cctatagtga cttttgtatc gccagctgtg ccaaaaaact agagaacata
     9181 gaaatcgctg aaacctacaa ggctgcttca caaaattacc gccagctatt tgacgctgag
     9241 acaggttaca tgcgagcacg agacaatcaa ggaaactttc accctgactt ctctccttat
     9301 agttgggggc gagactacgc tgaatgctct gccattcaag ctactttagg cgttctccac
     9361 gacatccctg gcttaatcca actgatgggt ggaaaagaaa cctttagcaa ctatcttttg
     9421 aaagcctgtc aagatgctcc cctctttgaa acaacaggct atggttacga aattcacgaa
     9481 atgagcgaga tggctactgc tccttttgga caaatagcca tttccaacca gcctagtttc
     9541 cacattcctt atctcttccg ctacagcgat taccctgact acactgccct tcttatcaag
     9601 acgctccgtc agaaagcttt tcacccaagt tgggaagcct atcctggaga tgaagacaat
     9661 ggtagtctct ctgcttggta catctggtca gctctcggat tttatccaac ctgtccagga
     9721 aaaccaagct atgatctcgg aatccctctc tttgaccatc tacgagtcta cctagctaaa
     9781 gaaaataaat ggctagatat tcatactgag caaaaccaca gccattttaa ctttgtcaaa
     9841 gaatgccgac tggacaaaac actcgtatca actattcaac accaagacct cctaaaagct
     9901 gaacaactaa ctttcaccct cagctggcta ccaaatcact aataaccaaa agaacctttg
     9961 catcgcgcaa aggttcttct tttatgaaac ttggacttga agttgatcca caagctgacc
    10021 atccaccgtt aggttcagat agaaatccaa ggttttatct cctgcgaaat acaaggttgg
    10081 tagcgtcagc cttttttcag tctcaccagc ttgaaacgca aggacataaa tctcgtcctg
    10141 ataggcgaca ccatggacac ctgtccccgg ttgaatcgaa atcttagcct ctaaagagga
    10201 gctactttca ctacgttcaa tcctaaaatg aagagtctct ccctttgcca cagctaggct
    10261 tttttctgca aatgctagtt cctgaacaac ttcttttttt gataacccag gagttttata
    10321 aagggaaatc ttggtcaata cagggataga ttgtgcctcg gtaatcatca cgcgcacctt
    10381 ttgtgcctct actagcggac ctcgcaagag gcgcttatga ccaactgtga agcccctgcc
    10441 aaactcctgc cagacaccat ccacctctac ttgcacatga aaagcagcga ttcgttgccc
    10501 tagcttcaaa tcttctctta actcaattac atcaaaagtt ttaggagaac ctaaatcgag
    10561 ttctaactgg atgggcaagt ctgcatcgct tgcccaagag ctggtctcaa ggccgtctgt
    10621 caaatggcga caatcaaagt ctgcggaaag agctggacca gatacctcag ctcccagagc
    10681 caaatcttct ttatagagct cattgcgata ggtcgcaaat tcataaagtc gttcaatatc
    10741 ctttgcatca aagagcccag cttgattcgg cggaatatta agcaagagtg gagttcctcg
    10801 ccctactgag tgaaagtaga tttcgaccaa ctcctcgaga gacttaggat cctgatcctc
    10861 atggtagaac cagcctggac ggatggaaac atctgcctct ccgattgaaa aaatcgtgcc
    10921 cgagggatcc ccgtgctgaa gatagttcag ctctgcttct gttcctagtt tatcaagatt
    10981 caccttttgc cacagtggat cacctgcata ccctcgttca ttgccaatcc agcggatact
    11041 ggtgccttct gttgaaaaaa tcaagcaatc gccctgcagg tcacgaatgg tttcaaacca
    11101 tttttcaaat tcataattaa ccttttgcgc gccctctcct ctggcaccat ccatccaaac
    11161 ctcagcgaac ttaccagcat tcccatagtt aggatttgat aagatttcct tcagctgagc
    11221 cagataatag gcattgtagt ccgcttctcg gtccacatga tagaggggac tatgggcatc
    11281 ccacggtgac aggtagaccc ccatatccat atcaaactct gtggcagctt gggatacttc
    11341 aaggagcaag tcgccctttc ctctcctcca aggactgacc ttaaccgaat aatctgtgtg
    11401 agctgtcgga taaaggacaa agccatcgtg gtgcttgacc accaaaatca actttttgaa
    11461 gcccgtttcc ttgagcacgc gaacccactc acgtgcgtct aactgtgtcg ggttaaagcg
    11521 ttcaggatcc tcctgacctg ttccccattc ctgattataa aaagtattag gtccaaaatg
    11581 gataaaggct gctagttcat ctcccagata agctagctga gtctgacttg gtaacggtct
    11641 atgcggtttg attttcttca tcgttatata tctccttctt ttgcaacttc tcttgttcac
    11701 aaggttatcc acaggttgtg gataataaac catgatctaa cagccagctc tttccaactc
    11761 caactggtag gctcaggatt tttctcctca atattccctc ttcaaacagc tctgtcatat
    11821 ggttctgcta acacctctca gaatgctact ctccataatt attctcaggc ttctttcagg
    11881 tcattgtgtg aagaccattc actctccaga cttttccaca gattgtgaat aagtgattat
    11941 tctaaactat cacctcgtcg actataacaa aaaataaaaa gagggtcaat aagataatta
    12001 tctcttccta aatactcaga aaagattcta taatttgtac aattgaacaa gaaatgcttc
    12061 tccaaaatct atcttcaaaa gaatgttgca tctttttatc ctttcacaca aacatttctc
    12121 tttatacaga tccaagtgat aaccagatga taattggaca ctttttagtc atttgtatat
    12181 tttttgaaaa attttttaaa aaaattttat atcctcttta atagaagttt atgcatttgt
    12241 atctatgtat tctatcctat gttatgtata ataacataaa attgtgctaa acatcataat
    12301 ttatattatt ttccgagaaa ttatagccat tgacattacc taggaattgc gttataacat
    12361 acatgtaagc ggtacccaaa atggtgccaa gtcaaaattt ttaaggagga aaatacatgt
    12421 cttctcatcc aattcaggtc ttctcagaaa ttgggaaact gaaaaaagtt atgttgcacc
    12481 gtccaggcaa ggagttagaa aacttgttgc cggactatct tgaaaggctt ctttttgatg
    12541 atattccttt cttggaagat gctcaaaaag aacatgatgc atttgcccaa gctcttcgcg
    12601 atgaaggaat tgaggttctc tacctagaac aactcgctgc tgaatcattg acctctccag
    12661 aaatccgcga tcaatttatc gaggaatact tagacgaagc caacatccgt gatcgtcaaa
    12721 ccaaggttgc tattcgtgaa ttgcttcacg gcatcaagga caaccaagaa ttggttgaaa
    12781 aaacaatggc tgggattcaa aaagttgaat tgccagaaat tcctgacgaa gctaaagatc
    12841 taactgactt agttgaatca gattatccat ttgcaattga cccgatgcca aacctctatt
    12901 tcactcgcga cccatttgca acaattggaa acgccgtatc gcttaaccac atgtttgcag
    12961 acactcgtaa ccgtgaaaca ctctacggta agtatatctt caaataccac ccaatctatg
    13021 gcggaaaagt ggatttggtc tacaaccgtg aagaagatac gcgtatcgaa ggtggagacg
    13081 agttagttct ttctaaagac gtccttgcag taggtatctc tcaacgtaca gacgcagctt
    13141 ctatcgaaaa acttttggtc aacatcttca agaaaaatgt tggcttcaag aaagttttgg
    13201 cctttgaatt tgctaacaac cgtaaattca tgcacttgga tactgtcttc actatggtag
    13261 actatgacaa gttcactatt cacccagaaa tcgaaggcga ccttcacgtt tactcagtta
    13321 cttacgaaaa cgaaaaactt aaaattgttg aagagaaagg tgacttagct gaacttcttg
    13381 ctcaaaacct tggtgtagaa aaagttcatt tgattcgttg cggtggtggc aatatcgtag
    13441 cagctgcgcg tgaacaatgg aacgacggtt ctaacacttt gaccatcgca cctggtgtgg
    13501 tagttgttta tgaccgcaat accgtgacca ataagatttt ggaagaatac gggcttcgct
    13561 tgattaagat tcgcggaagt gaattggttc ggggccgtgg tggacctcgt tgtatgtcta
    13621 tgccatttga acgtgaagaa gtgtaatcgc tgttcggcat tcgtcaatag aaaatgtaaa
    13681 aaatagaaag aggaaataat aaaatgacaa attcagtatt ccaaggacgc agcttcttag
    13741 cagaaaaaga ctttacccgt gcagagttag aataccttat tggtctttca gctcacttga
    13801 aagatttgaa aaaacgcaat attcaacacc actaccttgc tggcaagaat atcgctctcc
    13861 tatttgaaaa aacatctact cgtactcgtg cagcctttac aactgcggct atcgaccttg
    13921 gtgctcaccc agaatacctc ggagcaaatg atattcagtt gggtaaaaaa gaatctactg
    13981 aagatactgc taaagtattg ggacgtatgt ttgacgggat tgaattccgc ggattcagcc
    14041 aacgtatggt tgaagaattg gcagaattct caggcgttcc agtatggaac ggtctaactg
    14101 acgaatggca cccaactcaa atgctcgctg actacttgac tgttcaagaa aacttcggtc
    14161 gcttggaagg cttgacattg gtatactgtg gtgatggacg taacaacgtt gccaacagct
    14221 tgctcgtaac aggtgctatc cttggtgtca atgttcacat cttctcacca aaagaactct
    14281 tcccagaaaa agaaatcgtt gaattggcag aaggatttgc taaagaaagt ggcgcacatg
    14341 ttctcatcac tgaagatgct gatgaagcag ttaaagatgc agacgttctt tacacagacg
    14401 tttgggtatc aatgggtgaa gaagacaaat tcgcagaacg tgtagctctt cttaaacctt
    14461 accaagtcaa tatggactta gttaaaaaag caggcaatga aaacttgatc ttcctacact
    14521 gcttgccagc attccacgat actcacactg tttatggtaa agacgttgct gaaaaatttg
    14581 gtgtagaaga aatggaagta acagacgaag tcttccgcag caagtacgct cgccacttcg
    14641 atcaagcaga aaaccgtatg cacactatca aagctgttat ggctgctaca cttggtaacc
    14701 tttatattcc taaagtataa ttttagataa taaaccgtct accaacagct atgagggctg
    14761 cgactaatag ctttagtccg gtcctctttt atgtaatggt aatctattat ttcttataaa
    14821 atatgtgaaa aatcattaaa ttgaaatcta aacgcattct attgagtgtg ataaaggaga
    14881 atttatggca aatcgtaaaa ttgtagtagc tttgggagga aatgcgattc tttcttctga
    14941 cccatcagca aaggctcaac aagaagcttt agttgaaaca gctaagcatc ttgtaaaatt
    15001 gattaaaaat ggagatgatc tgattatcac tcacggtaat ggacctcaag ttgggaatct
    15061 cttgctccaa catttggcat cagactctga aaagaaccct gccttcccac tcgactcact
    15121 tgtcgctatg acagaaggta gcatcggttt ctggttgaaa aatgctttgc aaaatgctct
    15181 cttggatgaa ggcatcgaaa aaaatgttgc ctctgttgta acgcaagttg tcgtagataa
    15241 aaatgatcca gcttttgtta acttgagtaa accaatcggt cctttctatt cagaagaaga
    15301 agcaaaagca gaagccgaaa agagcggagc gactttcaag gaagatgctg gccgtggctg
    15361 gcgtaaggtc gttgcctcac caaaacctgt tgacatcaaa gaaattgaaa ccatccgtac
    15421 tcttttaaat aatggtcaag tcgtcgtagc tgcaggtggt ggcggtattc ccgtcgtcac
    15481 agaaaacaat ggacatttga ctggtgtcga agcggttatt gataaagact tcgcttccca
    15541 acgtttggca gaattggttg atgcagacct cttcatcgtt ttgacaggtg tagattatgt
    15601 atttgttaac tacaacaagc caaaccagga aaaattggaa catgtgaatg ttgcccagct
    15661 ggaagaatat atcaaacaag atcagtttgc accaggtagc atgcttccaa aagtagaagc
    15721 agctatcgct tttgtcaatg gtcgtccaga aggaaaagca gttattactt cccttgaaaa
    15781 tctaggcgcc ttgattgaat ctgaaagcgg aacaattatt gaaaaaggat aagttgtttt
    15841 actaataaga tgtattctat ttctagtatc tttatatcaa attagaaatt attcttgaaa
    15901 acatgtacaa tatttcaaaa gatactagtt ttagacttta atatggtaaa acaaatataa
    15961 atagaaagcg ttttcttgaa tgtttattta agaaagtagt tggtttttta cactttgtta
    16021 gacatcagga ggaaaaacaa atgagtgaaa aagctaaaaa agggtttaag atgccttcat
    16081 cttacaccgt attattgata atcattgcta ttatggcagt gctaacttgg tttatccctg
    16141 cgggggcctt tatagaaggt atttacgaga ctcagcctca aaatccacaa gggatttggg
    16201 atgtcctcat ggcaccgatt cgggctatgc taggtactca tccagaggaa ggttcgctca
    16261 ttaaagaaac gagcgcagcg attgatgtag ccttcttcat ccttatggtt ggtggtttcc
    16321 ttggcattgt caacaaaact ggtgctcttg acgtagggat tgcctctatc gtgaagaagt
    16381 ataagggccg cgaaaaaatg ttaattttgg tactgatgcc tttgtttgcc ctcggtggta
    16441 caacttatgg tatgggtgaa gaaacaatgg ccttctatcc actccttgtg ccagttatga
    16501 tggccgttgg ttttgatagc ctgactggtg ttgcaattat tttgctcggt tctcaaatcg
    16561 gctgtttggc atctactctg aatccatttg cgacaggtat tgcttcagcg actgcgggag
    16621 ttggtacagg ggacggtatc gtacttcgtc tgatcttctg ggttaccttg actgctctta
    16681 gtacttggtt tgtttaccgt tatgcggata agattcaaaa agatccgact aagtcactgg
    16741 tttatagtac tcgcaaagaa gatttgaaac actttaacgt agaagaatct tcatctgtag
    16801 aatctacact tagcagcaaa caaaaatcag ttctcttctt atttgtgttg acattcatct
    16861 tgatggtatt gagcttcatt ccatggacag accttggcgt taccattttt gatgacttta
    16921 atacttggtt gactggtctt ccagttattg gtaatattgt cggttcatct acttctgcac
    16981 taggtacttg gtacttccca gaaggcgcaa tgctctttgc ctttatgggt atcctgattg
    17041 gtgttattta tggtcttaaa gaagataaga ttatctcttc cttcatgaat ggtgctgctg
    17101 acttgctcag tgttgccttg atcgtagcga ttgctcgtgg tattcaagtt atcatgaacg
    17161 acggtatgat taccgataca atcctcaact ggggtaaaga aggcttgagc ggtctatctt
    17221 cacaagtctt tatcgttgta acttatatct tctatctacc tatgtcattc ttgatcccat
    17281 cttcatctgg tcttgccagc gcaactatgg gtatcatggc tccacttgga gaatttgtaa
    17341 atgtccgtcc tagcttgatt atcactgctt accaatctgc ttcaggtgtc ttgaacttga
    17401 ttgcaccaac atctggtatt gtgatgggag ctcttgcact tggacgtatc aacattggta
    17461 cttggtggaa attcatgggc aaactcgtag tcgctattat tgtagtgacc atcgcccttc
    17521 ttctccttgg aaccttcctt ccattcctat aaaatagtga gtgaggtgat tccatgaaaa
    17581 tagatataac aaatcaagtt aaagatgaat ttcttatatc attaaaaacc ttgatttcct
    17641 atccttcagt actcaatgaa ggagaaaatg gaacaccttt tggacaagca atccaagatg
    17701 tcctagaaaa aactttagag atttgtcgag acataggttt cactacctat cttgacccta
    17761 aaggttatta cggatatgca gaaatcggtc agggagcaga gcttctggcc attctctgtc
    17821 atttggatgt tgttccatca ggtgatgaag cagattggca gacaccgcca tttgaagcaa
    17881 ctatcaaaga cggctgggta ttcggacgtg gtgtccaaga tgataaaggc ccttcgctcg
    17941 cagctctcta tgcagtaaaa agcttgctgg accaaggtat tcagttcaaa aagcgcgtac
    18001 gctttatctt tggtaccgat gaggaaaccc tctggcgctg catggcacgc tacaatacca
    18061 tcgaagaaca ggccagtatg ggctttgcac ctgactcatc ttttcctctg acctatgctg
    18121 aaaaagggct tctacaggtc aaacttcatg gcccgggatc ggatcaacta gagcttgaag
    18181 taggaggcgc ctttaacgtt gtaccagaca aggccaacta ccaaggtctc ctctatgaac
    18241 aggtttgtaa cggtctcaaa gaagctggtt atgattacca aaccactgaa caaaccgtaa
    18301 cggttctcgg agtgccaaag catgctaagg atgctagtca aggtatcaat gctgtcatcc
    18361 gactagctac cattcttgct cctctccaag aacaccctgc tctcagtttt cttgcaacac
    18421 aagcaggtca agacggcaca ggaagacaaa tctttggtga tatagcagat gaaccttctg
    18481 gtcacctatc ctttaatgtc gcaggtctca tgatcaatca tgaacgttct gaaatccgta
    18541 ttgacattcg gactcctgtc ttagctgaca aggaagaact agtagagttg cttacaagat
    18601 gtgcacaaaa ctaccaactc cgctacgaag agtttgacta tctagcgcct ctatacgtcg
    18661 caaaagacag taaactcgtt agcacactga tgcaaatcta ccaagaaaag actggcgata
    18721 acagtcctgc tatttcatcc ggtggtgcca cttttgctcg caccatgcca aattgtgtag
    18781 ccttcggcgc cttattccca ggagcgaagc agacagaaca tcaggcaaat gaatgtgccg
    18841 ttctagaaga tttgtaccgt gctatggata tttatgccga agccgtctat cgacttgcaa
    18901 cttaatcagg caactgtttc tacaaaaaaa atcgaccgat taatgaactg caccccaaaa
    18961 gttagacaga a
//
LOCUS       DBEJAQ010000040        17022 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.040, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000040 DBEJAQ010000000
VERSION     DBEJAQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.040"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            168..857
                     /locus_tag="ACL297_001542"
     CDS             168..857
                     /locus_tag="ACL297_001542"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGR0071787.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPK
                     EIAQRFINVIK"
     gene            882..1799
                     /locus_tag="ACL297_001543"
     CDS             882..1799
                     /locus_tag="ACL297_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGR0071788.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            1827..3368
                     /locus_tag="ACL297_001544"
     CDS             1827..3368
                     /locus_tag="ACL297_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HGR0071789.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAIAIVYSYTGGLKSVLWTDFIQGVVLISGVVVALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLAIATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGVTGL
                     ILAAIYAAAQSTVSTGLNSVATSWTLDIQDVISKNMSEDRRTKIAQFVSLAVGLFSIV
                     VSIIMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTENKVSAPKYTTIHDISEIKADSS
                     WEVRH"
     gene            3394..3852
                     /locus_tag="ACL297_001545"
     CDS             3394..3852
                     /locus_tag="ACL297_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGR0071790.1"
                     /translation="MIISEQSDFRRYASVNKHFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            3854..6076
                     /locus_tag="ACL297_001546"
     CDS             3854..6076
                     /locus_tag="ACL297_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase domain-containing protein"
                     /protein_id="HGR0071791.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTSISEVQHKSGNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVTRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPAPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIREHGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTQVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQISIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6116..7225
                     /locus_tag="ACL297_001547"
     CDS             6116..7225
                     /locus_tag="ACL297_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGR0071792.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            7382..8272
                     /locus_tag="ACL297_001548"
     CDS             7382..8272
                     /locus_tag="ACL297_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGR0071793.1"
                     /translation="MKIIGIDIGGTTIKADLYDDFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYVLNYSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTSEIEKRF
                     GLSTFVENDVNCAALGELWEGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLSSGDKVAKETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNVVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            8297..8620
                     /locus_tag="ACL297_001549"
     CDS             8297..8620
                     /locus_tag="ACL297_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071794.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELISIESNLEMKQQNLIDEVNKTIKENDAKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            8610..10601
                     /locus_tag="ACL297_001550"
     CDS             8610..10601
                     /locus_tag="ACL297_001550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HGR0071795.1"
                     /translation="MAISQMKRISLLFTKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFSTIQISHTSNSHHGVIEGNDALNYLMNKQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERNIQSNNEEIERLIKW
                     EKLEIIPAHLEQFSFCRGKVGTIPRTVDNRLYNNFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGIMLALKIFHLPSVTKKFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ITTTSDVMTILVVSVVFGFITVFVGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDIRPLFVLGKWVSIFNAVGILIASIIQAKSLSGIGSGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            10617..11093
                     /locus_tag="ACL297_001551"
     CDS             10617..11093
                     /locus_tag="ACL297_001551"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HGR0071796.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            11127..11708
                     /locus_tag="ACL297_001552"
     CDS             11127..11708
                     /locus_tag="ACL297_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071797.1"
                     /translation="MSNLENLRESVIEQAHEKGSMKLLDSKKKIDDEFEMQKSVIVKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            11773..12780
                     /locus_tag="ACL297_001553"
     CDS             11773..12780
                     /locus_tag="ACL297_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HGR0071798.1"
                     /translation="MDTNLFSKINTTISVKENDFITEEKFQKIIQSKDTETLAFILEA
                     TPYHLSIDVLENPSQTEISLMTKLVNDYRWAYAESPSDKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELGEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKTLDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYG
                     S"
     gene            12770..13090
                     /locus_tag="ACL297_001554"
     CDS             12770..13090
                     /locus_tag="ACL297_001554"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HGR0071799.1"
                     /translation="MEANKYKIGIIGSHDIILPFSMIGFDIFSAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            13153..14928
                     /locus_tag="ACL297_001555"
     CDS             13153..14928
                     /locus_tag="ACL297_001555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HGR0071800.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            14929..16314
                     /locus_tag="ACL297_001556"
     CDS             14929..16314
                     /locus_tag="ACL297_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HGR0071801.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            16337..16948
                     /locus_tag="ACL297_001557"
     CDS             16337..16948
                     /locus_tag="ACL297_001557"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HGR0071802.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         5706 a         2420 c         3425 g         5471 t
ORIGIN      
        1 gtgtattgaa tctataacag tacgccttaa atgctaaaat atttctataa attaatttaa
       61 ctttcctgat agggctgttc atatcttatt tcaattcact atataaataa aattgtagta
      121 gatgttattg aattagtaaa aaaaataaaa aaacggaggt tttctgaatg gaaactaaaa
      181 aaattaaaaa tttaaaaggt caaataattg tttcctgcca agcgttagaa ggagagccac
      241 tttatactcc aaatggagga gtgatgccgt tattagcaaa agccgccttt caagcaggcg
      301 ctaaaggtat tcgtgctaat tctgtccgcg atatttcaga aataaaagaa gaagttgact
      361 taccaattat aggtatcata aaaagagatt acgatggctt tgaacctttt atttcagcaa
      421 ctatgaaaga gattgatgaa ttagttagtg aaggagtaga cattttagct ttggattgta
      481 ctaatcgttc aagaccaggt tatgataata ttactgactt tattcatgat attaaagtca
      541 agtatccaaa ccaattatta atggctgata tttctacttt tgaagaaggg aaagtagctg
      601 ctgaatcggg agtagatttt gtgggtacga cattatctgg ttatacacca tatagcccta
      661 aaaaagataa tcctgatttt gagttagttg aaagattggt aaaagagctt gacgtaccag
      721 ttattgctga aggaagaata agtactccgg aacaagctag aaaaatgtta gatttaggtg
      781 cttacgcagt ggttgttgga ggagctatca ctcgtccaaa agaaattgct cagcgattta
      841 ttaatgtcat caaataaaaa acaatataaa ggagaaatct gatgaaagat ttaactaaat
      901 acaaaggcgt tattcctgca ttttatgctt gctatgatga aaatggtgaa attagccaag
      961 aacgtgtaaa atctctggtg caatatttca ttgacaaagg tgtaaaaggt atctatgtaa
     1021 atggttcttc aggtgaatgt atttaccaaa gtgtagaaga tcgtaaacaa attattgaag
     1081 ctgttatgga agttgctaaa ggtaaattaa cagttatcaa ccatgttgca tgtaataaca
     1141 cgaaagatag tattgaattg gcaaaacatt cagaaagtgt tggagtcgat gctattgctg
     1201 ctatcccacc tatttacttc aaattgccag aatattcaat tgcagcatat tggaatgcaa
     1261 tgagtgaagc tgcgccaaat acagatttta tcatttataa tattccacaa ttggcagggg
     1321 ttgcgttgac tggtagtttg tatgcaacaa tgcgtcaaaa tcctcgtgtg attggagtta
     1381 aaaattcttc tatgcctgta caagatattc aaatgtttgt agctgcaggt ggagaagatt
     1441 acattgtatt caatggtcca gatgaacaat atcttggtgg tcgcttgatg ggagcagaag
     1501 ctggtattgg tggtacttat ggcgttatgc cagatttgtt cttgaaattg gaaagtttga
     1561 ttcaagaacg agatttagat actgctaaaa aacttcaata tgctatcaat gaagttatct
     1621 ataagatgat atcaggtaag gcaaatatgt atgctgtagc aaaagaggtt ttgcgtctaa
     1681 atgaaaaact tgatttaggt tctgttcgtc aacccttaga agcattggca gaaggtgact
     1741 tggaagttgc aaaacaagca gcagaactta ttcaacaagc acgaaaagaa tttttataat
     1801 aaataaaatc aatatggaga tatgttatgg gcacaacagg atttacaata attgacttaa
     1861 ttatcttgat tgtttattta cttgcggtgt tggttgcagg tatctatttc tctaaaaaag
     1921 agatgaaagg aaaagagttc tttaaaggag atggttcggt tccttggtat gttacttcgg
     1981 tatccatttt tgccacaatg ctcagtccga tttccttctt gggactcgct ggtaactctt
     2041 atgcaggtag ctggatttta tggtttgctc aattagggat ggttgtagct attccactga
     2101 caattcgttt tatcttacct atctttgcac ggatagacat cgatacggca tatgattact
     2161 tggataaacg ttttaattct aaagcacttc gtattatttc agcactcttg tttattattt
     2221 atcaattggg acgtatgtct atcattatgt acctcccatc agctggttta tcagtattga
     2281 caggaattga catcaatatt ttgattattt tgatgggtgc aattgcaatt gtttattctt
     2341 atactggtgg tctaaaatcc gtattatgga cagactttat tcaaggtgtg gttctgatta
     2401 gtggtgtcgt tgtagcttta tttgtactga tggctaatat taaaggtggc tttggtgcag
     2461 tagcagaaac attagcaaac gggaaattcc ttgctgcaaa tgaaaaactt ttcgatccta
     2521 acttgctttc aaactccatc ttcttaattg tgatgggttc aggctttaca atcttgtctt
     2581 cctatgcttc atctcaagat ttggttcaac gttttactac aacacaaaat attaagaaac
     2641 ttaataaaat gttgttcaca aatggtgtct tgtcacttgc aattgcaaca gtcttttact
     2701 tgatcggtac aggcttgtac gtattctatc aagtacaaaa tgcaggtagt gcagctagca
     2761 atatccctca agaccaaatc tttatgtact ttattgcata ccagttacca gtaggtgtca
     2821 caggtttgat cttggcagcg atttatgcag cagctcaatc aactgtttca acaggtttga
     2881 actctgttgc aacttcatgg acattggata ttcaagatgt catttctaaa aatatgtcag
     2941 aggatcgtcg tacgaaaatt gcacaattcg tatctctagc agtaggttta ttctcaattg
     3001 ttgtttccat tatcatggct cattcagata ttaaatctgc atacgaatgg ttcaatagtt
     3061 tcatgggact tgtacttggt ctacttggtg gtgtattcat tcttggtttt gtttctaaaa
     3121 aagcaaataa acaaggtgct tatgcagcgc tgattgtatc aaccatcgtt atggtattta
     3181 ttaaatactt ccttcctcca acagctgtta gctactgggc atattcattg atttcaattt
     3241 ctgtatcagt agtttcaggt tatattgtat ctgttcttac tgaaaataaa gtatctgcac
     3301 ctaaatatac aacgattcat gatatttcag aaattaaagc ggattcaagt tgggaagttc
     3361 gtcactagtc actaatagaa aagagaaaat aatatgatta tttctgaaca atctgatttt
     3421 agacgatatg cttctgtcaa taaacatttt tcaaaagtct gtgatttttt agaaaataca
     3481 aatttgacag atttagttga tggaaaaatt gatattgacg gggaaaatgt ttttgcaaat
     3541 tgtatgactt atctagctga tggagttcca ggagatattt ttgaaactca taagaaatat
     3601 ttagatattc atattgttgt tgaaaataca gaaaaaatgg cagtaacttc cccagttcgt
     3661 gctcagtcac gtgtaccttt tagcgaagag aaggatattg ctttttacga tagcaaagac
     3721 tatcaaattg ttgaattgct tccagggaat atgttggtga cttttgaaga agatcttcac
     3781 caaccaaaga ttcattgcaa tgatgaaact gttaaaaaac ttgttatcaa agttttaaac
     3841 gaggaaaaat aaaatgaaaa aaaatattaa acaatatgta accttaggta ctgtagtggt
     3901 attatcagca tttgttgcta actcagttgc agctcaggag actgaaactt ctgaagtatc
     3961 aacaccagag ttggtgcaac ctgttgcacc aacgacttcg atttcggaag tacaacataa
     4021 atcgggtaac tcttcggaag ttactgtaca acctcgaaca gttgaaacta ctgttaagga
     4081 tccatcttct acagcggaag aaactcctgt cttagaaaaa aataatgtta ctttaacagg
     4141 gggcggagaa aatgttacta atgagttaaa ggataaattt actagcggtg actttactgt
     4201 agtgattaag tacaatcagt ctagtgagaa aggtttacaa gctctgtttg gaatatctaa
     4261 ttctaaaccc ggtcaacaaa atagttatgt agatgtgttc cttagagaca atggtgaatt
     4321 gggaatggaa gcgcgtgata cttcttccaa taaaaatcac ctagtaacca gacctgcttc
     4381 agtttggggt aagtacaaac aagaggccgt gacgaacact gttgcagtag tagcagattc
     4441 agtcaaaaaa acgtattctt tatatgcaaa tggtacaaaa gtagtagaaa agaaagtgga
     4501 taacttccta aacatcaagg atattaaagg tattgattac tatatgcttg ggggagtgaa
     4561 acgtgcagga aaaacggcgt ttggttttaa cggaacacta gaaaatatca aattctttaa
     4621 tagtgcattg gatgaagaaa ctgttaaaaa gatgacaaca aacgctgtta ctggacattt
     4681 aatttatacg gctaatgata caacaggttc taactatttc cgtattccag ttctgtatac
     4741 ttttagcaat ggtcgggttt tttcaagcat tgacgctcgt tacggtggaa ctcatgattt
     4801 cttgaataaa attaatattg ctacaagtta tagtgatgat aatggtaaga catggactaa
     4861 accaaaatta acattagcat tcgatgattt tgcgccagca ccattagaat ggcctcgtga
     4921 tgttggtgga cgtgacttac aaatcagcgg tggtgcaacc tatattgact ctgttattgt
     4981 tgaaaaaaat aacaaacaag tactcatgtt tgctgatgtt atgcctgctg gagtaagttt
     5041 tagagaagct actagaaaag attcaggtta taaacaaatt gatggtaatt attaccttaa
     5101 attaaagaaa caaggtgata ctgattataa ttatactatt cgtgagcatg gtactgtata
     5161 cgacgatcgt accaacagac caactgaatt ttcagtagat aaaaatttcg gtattaaaca
     5221 aaatggtaat tatttgacgg tagagcaata ttcggtttca tttgaaaata ataaaaagac
     5281 tgaatatcgt aatgggactc aagtccatat gaatatcttc tacaaagatg cattattcaa
     5341 agtagtgcca acgaactata ttgcatatat ttctagcaat gatcatggcg aatcttggtc
     5401 tgcaccaact ttattacctc ctataatggg acttaatcgg aatgcgcctt atttgggtcc
     5461 tggacgtgga atcattgaaa gctcaactgg acgtattctt attccgtctt acactggtaa
     5521 agagtctgca ttcatttata gtgacgataa tggagcgtct tggaaagtta aagttgtgcc
     5581 acttccttct agttggtcag cagaagcaca atttgtagaa ttgagtccag gagtaattca
     5641 agcatacatg cgtacaaata atggtaaaat tgcatattta acaagtaaag atgcaggtac
     5701 tacttggagt gcgccggaat atttgaaatt tgtttcaaat ccaagttatg gaacacaatt
     5761 atcaatcatc aattatagtc aattgattga tggtaaaaag gctgtcattt taagtactcc
     5821 aaactccaca aatggtcgta aacacggaca aatttcgatt ggtctaatta atgatgataa
     5881 tacaattgat tggcgttatc atcacgatgt tgattatagt aactatggat actcatattc
     5941 aacattgaca gagttaccaa atcatgaaat tggattgatg tttgaaaaat ttgattcatg
     6001 gtctcgtaat gaacttcata tgaaaaatgt tgtaccatat ataacattta agattgaaga
     6061 tttgaaaaag aattaaagct gaaatttaaa aatatataga aagaggataa aaattatggt
     6121 aaattacggt attgttggag ctggatattt tggagctgat ttggctcgct caatgaacaa
     6181 aattgaagat gcaaaagtgg ttgcggtatt tgacccaaat catggagaag aagttgctca
     6241 agagttggga tcagatgttt gtgcaagttt agatgaactt gtagcacgtg aagatattga
     6301 ttgtgtgatc gtagcttcac ctagctacct tcaccgtgaa cctgttgtga aagctgctca
     6361 acatggcaaa cacgtatttt gtgaaaagcc aattgcattg tcttatgaag attgtaaagc
     6421 catggttgac gcatgtaaag aaaataatgt tatctttatg gctggtcaca tcatgaactt
     6481 ctttaatggt gtacaccatg ctaaagaatt gattactcaa ggtaaaatcg gtaaagttct
     6541 ttattgccat gccgctcgta caggttggga agaacaacaa ccaactgtat catggaagaa
     6601 acttcgttct caatctggag gacatttgta ccaccatatt catgaattag attgcattca
     6661 gttcatcatg ggaggacttc ctgaaaaagc gacaatggta ggaggcaatg tatatcataa
     6721 aggtgaaaac tttggtgatg aagatgatat gctcattgta aacctagaat actctgatga
     6781 tcgttatgct gttttggaat atggtaatgc tttccgttgg ggtgaacact acgtcttgat
     6841 tcaaggaact gaaggagcta tcaaacttga cttgttcaat actggaggta ctcttcgtgt
     6901 taaaggtgaa ggagaatcac acttcttagt acatgaaact caagaggaag atgatgaccg
     6961 tacagctatc tataccggtc gtggtatgga tggagcaatt gcgtacggta aaccaggagt
     7021 acgttgccca ttatggttac aaacatgtat tgataaagaa atggaatatc tacatgacat
     7081 cattaaaggt ggagaaatta cagaagaata tgaaaaactt ctcaatggtg tagctgcttt
     7141 agaatcaatc gctaccgctg atgcatgtac tttatcagtt aaagaagatc gaaaagtaag
     7201 tctttcagaa atcacaaatg cttaactatt gtaaaacaga atagtaaatt cttgtcatta
     7261 tataatttct aaagttctgt gatacaactc attgaataaa gaaatagaga tgggactggg
     7321 ataatgccca gtcccatttt ttatcaaaaa gtaatgagat caaaaatgtg ggagtgatga
     7381 aatgaagatt atagggatcg atattggcgg aacaacaatt aaggcagatt tatacgatga
     7441 ttttggaacg agtttaaatc agttcaaaga gatagaaaca attattgact atgatttggg
     7501 aacgaaccag atattaaatc aggtctgtga tttaattggt gagtatgttt taaattattc
     7561 aattgatggt gttgggattt ccactgctgg agttgttaat gctaatacag gagaaatcat
     7621 ctatgcaggc tatacaatac cagggtatat cggagtaaac tttacttccg aaatagaaaa
     7681 acgttttggg ttgtctactt ttgttgaaaa tgatgttaat tgtgctgcat taggtgaatt
     7741 gtgggaggga caagccaaag ataagaaaaa tgtagtaatg gttactattg gaacaggtat
     7801 aggaggcagt attattgtca atggacaaat tgttaacgga tttaactata ctgctggtga
     7861 agtaggctat attcctgtag gtaattcgga ttggcaaagc aaagcctcaa caaccgcatt
     7921 gattcattta tatcaaaaga agagcttgaa aactaatcaa actggacgta ctttctttac
     7981 tgatttaagt tctggagata aagtcgctaa agaaactttt gaaatttttg tagaaaatct
     8041 aacaaaaggt ttattaacta tttcttatct acttaatcca gaaattctca tattaggagg
     8101 tgggattctg gctaaaaagg atattttgtt acctgaaatt caaagttctt tagctaaaaa
     8161 tgcaatggat aataggtttt tgcctaaaaa tgttgtggca gctacattag gaaatgaagc
     8221 tggtcgtata ggagctgtaa aaaatttctt agatagaatt tctaataaat agtgtgaaag
     8281 ataaggaggt gtcacaatga ctaactctgt attttcgaca atgcaagata ttgagaatgt
     8341 tgcaaccgat attataaaat catatgataa tgagatttat acgtataaag ctgtctccca
     8401 agaagaattg gaaaaactag aaaaaagtta tgatgaaaaa agtcacgaag aattaatttc
     8461 aatagaaagc aatttagaaa tgaaacaaca gaacctcatt gatgaggtta ataaaacaat
     8521 caaggaaaat gatgcaaaga ttcagtatat ttcatcaagt aggagaggag aatttgtaga
     8581 aaaaattatt ggtagggtgg tagaaaaata tggctattag tcagatgaaa agaatttctc
     8641 tactatttac taagagtagt cttgatgatg ttttaaaaac tattcaagaa ctagagtcag
     8701 tgcagttccg tgatttaaag gttcaggata actggtcaga agcgctagaa aaagatgagg
     8761 ttgtattttc aactattcaa atttctcaca cttctaattc ccatcatggg gttattgaag
     8821 gaaatgatgc cttgaattat ttgatgaata aacaacaaca tttggaagca actgtagaga
     8881 aattacaaga atacctaccg aaagaaaaca cgtttaagtt attacagcaa cctccgataa
     8941 caacctctta tgaagaatta gaaaaatttg gtaaagctaa tgttgctgag ggtgttctta
     9001 aaaaagtgaa tcatcaaata aacagagttc atgaattaga aagaaacatt caaagtaata
     9061 atgaggaaat agagagatta ataaagtggg aaaaattaga aattattcct gcgcatttag
     9121 aacaattttc tttctgtaga ggaaaagtcg gaacaattcc aaggactgta gataatcgct
     9181 tatacaataa ttttttagaa aacaatattg aagttcaaga aatattttct aatgatagag
     9241 agtacggtgt tgttgttttc tatcagtcca gttgctctat tgattttgat gaatacttat
     9301 ttgaaccatt tgattattct agaaaggaat taccgaagca gcgagtagta gatttggatc
     9361 aagaaaacat gcagttaata actgaaaaag agaatattat cgcatcgttg caagattcaa
     9421 agaaaaattt gatagattta caatggcaaa ttgactatat tttatctatc tatgctcgtc
     9481 aaatctctaa gaataacctt ttgagcactc cgcatctagt tgcattagaa ggatggatag
     9541 aagaaactcg tattttatat tttataaaag ttatggatga gcgttttgga cattctattt
     9601 atatttatga atcggaaaca ttgtcggata atcaagatga aatacctatc aaattaacga
     9661 atcactcttt aattgaacca tttgaattat tgacagaaat gtatgctctg cccaaatatt
     9721 atgagaaaga tcctacacct gtattagcac cattttactt tacatttttt ggaatgatgg
     9781 ttgctgattt aggctatggt ttactattgt ttttaggaat aatgttagca ttaaaaattt
     9841 ttcatctacc ttcagtaact aagaaatttt taaaattctt taatatatta ggggtagccg
     9901 ttgcaatttg gggtggaatc tatggctcat tttttggata tgagttgcca tttcatctga
     9961 taactacaac ctcggatgtc atgactatat tagtagtgtc agttgtgttt ggatttatta
    10021 cagtatttgt aggtttgtta gcttcaggac tacaaaaagt aagaatgaag aaatatgcag
    10081 aagcatataa ttcaggattt gcgtggtgtg ttattctgct tggcttgtta tttattgctg
    10141 tcggaatgtt gatgcctgat attagaccgt tatttgtatt agggaaatgg gtatctattt
    10201 ttaatgctgt ggggattttg attgcttcta ttattcaagc caaaagcttg tcaggtattg
    10261 gctcaggatt gtttaatcta tataacattt catcttatat aggtgattta gttagtttca
    10321 ctcgattgat ggcattagga ttatctggag caagtatagc atcagctttc aatttaattg
    10381 ttggtttgtt tccggggaca ttggctaaac tgacaattgg attagtatta ttcattcttt
    10441 tacatgcgat caatattttt ctatcgttac tatcaggata tgttcatgga gcgcgtctga
    10501 tatttgttga attttttggt aagttttatg agggtggagg aaaaccattt caacctttga
    10561 aggcttctga gaaatatatt aaggttatta caaagaatta atggaggata tatataatgg
    10621 aacatttagc aacttatttt tcaacctatg gaggagcttt cttcgctgca ttgggaattg
    10681 tattggcggt tggattaagc ggtatggggt ctgcttatgg agttggtaag gctgggcaat
    10741 ctgccgcagc tttactgaaa gaacagccag aaaaatttgc ctcagctttg atattgcaac
    10801 tattgcccgg aacacaagga ttatatggtt ttgttattgg aattttgatt tggttgcaat
    10861 taactccaga acttcctttg gaaaaaggcg ttgcttattt ctttgtagct cttccaattg
    10921 ctattgtagg atacttttcg gctaagcatc aaggaaatgt agcagtagcg ggaatgcaaa
    10981 tcttggctaa aagaccaaaa gaattcatga agggagcaat tttggctgcc atggtagaaa
    11041 cctatgcaat tcttgccttt gtcgtatcat tcattttgac ccttcgtgta taagaaatta
    11101 atttgctatt caaaggaggt gtctaaatga gcaatttaga aaacttacga gaatctgtta
    11161 ttgaacaagc tcatgaaaaa gggagtatga aattattgga ttccaaaaag aagattgatg
    11221 atgaatttga aatgcaaaag tcggtcattg taaagaaaaa agaagctgaa catgaacgaa
    11281 agttaaaaga attacaacag aaatatcaaa taatttttca acaattaaaa aataaggaac
    11341 gccaatcaac gttagtatca aaacagaaaa tattaaaaga actttttcaa tctgctttaa
    11401 tagaaatgga atcttggagt gcagataaag aaatggagtt catctatcga attctggaac
    11461 gatattcaca acaagaggtc atagtaactt ttggggagcg gactttagct aaattcaatt
    11521 tggaacaatt agagaaattg aaattctctt ttccaaatca tttatttagt gaacagccta
    11581 tctcaaatga atcaggctta cttatttcaa taggtaaaat tgatgataac tatttgtata
    11641 aaacgttaat tggatcgatt tctaaggaag aaagttcaag tatcgcaaat caaattttta
    11701 tcaattaagg atgaaattgg ttaatccttc ttagaaattt ggagtattcc aataaaatta
    11761 gaaaggtatt ttatggatac taatcttttt tcaaaaataa atacgacgat ttcggtaaaa
    11821 gaaaacgatt ttattacaga agaaaaattt caaaaaatta tacaatccaa agatacggag
    11881 acattggcat ttatcttaga agcaactccc tatcatttat cgattgacgt tttagaaaat
    11941 cctagtcaga cagagatttc gctaatgaca aaattagtca atgattatag atgggcctat
    12001 gctgaaagtc cgtctgataa aattgtgact ttatttgctt tacgatatgt ttatcataat
    12061 atcaaagttt tattaaaatc taaggcggca attaagaaag atttttctaa attattaatt
    12121 ccaataggga tttttgatat agaaagttta aaacatttag tttcttcctt acattcagat
    12181 acacttcctg attttatggt tcatgaagta gaatcaattt ggaatgagta tgaaactttt
    12241 aataatattc gtgtacttga tgtcggagct gatctagcat attttaaaca tctgaaactt
    12301 ttatctaatg agttaggtga ggtgctgtct caggttattg ttgcaatgat tgacttttat
    12361 aatattatta ctgtaaaacg tggtttatct caaaataaga gtcatgggga tattttacaa
    12421 ttactttcag atgaaggaag tatttctgct aaagaattta tatacattgt agaaaatcaa
    12481 gaaatatttg tgtggttcaa taaaataaat ccaagcttag attcaatctt ttcaacttac
    12541 gaattgaaga tgcaggacgc aacaatttca tcttctgagt tagaattttt atgtgattta
    12601 ctattgtata aaactttaga tcaaggaagg tataatgtag atgggccgtt agttcttgct
    12661 agatatttat tgggatgtga gtttgaagta aagaatctta gaatgatcat atcagctctt
    12721 caaaatacaa ttccttttga atcaataaaa gaaaggatac gcccgcatta tggaagctaa
    12781 taagtataaa attggcataa ttggtagcca tgatattatt ttacccttta gcatgattgg
    12841 gtttgatata ttttctgcct atcaagaaca agaagctata aatactctaa gaaaattagc
    12901 tcaatctgat tatggtgtca tttatatcac tgaagacatt gcttcaatga tattagatac
    12961 aattcgccat tatgattctc aagttgtgcc tgctattatt ttattaccga ctcataaaca
    13021 aggtttaaat ttaggattaa aacgtataga ggataatgta gagaaagcag taggacacaa
    13081 tattttataa taatgtacaa aattgtctgt aatattattc tataattttt ggacttagta
    13141 aggagaataa ctttgactca agggaagatt ataaaagtat cgggacctct agttattgca
    13201 tcaggtatgc aggaggctaa tattcaagat atttgccgtg taggtaagct agggttaatc
    13261 ggtgaaatta ttgaaatgag aagagatcag gcatctatcc aagtctatga agaaacatct
    13321 ggtcttggtc cgggagaacc tgttgttaca actggagaac ctctctcggt tgaattaggg
    13381 ccaggattga tttctcaaat gtttgatggc atacaacgcc cattagatcg atttaaattg
    13441 gctactcata atgattttct agttcgtggg gtagaagttc caagtttgga tagagatatt
    13501 aagtggcatt ttgattccac tatagcaatt ggtcaaaaag tgagtacggg tgatattctt
    13561 ggaactgtca aggaaaccga ggtagttaat cataaaatta tggttcctta tggagtatct
    13621 ggagaagtcg tttctattgc atctggcgat tttacaattg atgaagttgt atatgaaata
    13681 aaaaaattgg acggtagttt ctataaagga acgcttatgc aaaaatggcc tgtccgcaag
    13741 gcgcgtcctg tttctaaacg tttaattcca gaagaaccat taatcacagg tcaacgagtt
    13801 attgatgcat tctttccagt aaccaaaggg ggagctgcag cagttcctgg accgtttgga
    13861 gcaggaaaga cagttgtaca acaccaagta gctaaatttg ccaatgttga tattgttatt
    13921 tatgtcggtt gtggagaacg tggaaatgaa atgacggatg tactgaatga gtttcctgag
    13981 ttgattgacc ctaataccgg acaatcaatt atgcaacgga cagttctgat tgctaatact
    14041 tcaaatatgc ctgttgctgc tcgtgaggct tcaatttata caggaattac catggctgag
    14101 tattttcgtg atatgggcta ctctgtcgcc attatggctg attcaacttc acgttgggca
    14161 gaagcgctac gtgaaatgtc aggacgtcta gaagaaatgc ctggtgatga gggttatcct
    14221 gcttatctgg gaagtcgtat cgctgaatat tatgaaagag caggacgttc tcaggttcta
    14281 gggcttccag aacgtgaagg aacgattact gctattggag ctgtatcgcc acctggtgga
    14341 gatatttcag aaccagttac tcaaaacact ttacggattg tgaaagtttt ttgggggctt
    14401 gatgctccgt tggcacagcg acgtcatttt cctgcaatta actggcttac atcttattca
    14461 ctatataaag acagtgtggg cacttatata gatggtaaag agaagacaga ttggaatagt
    14521 aaaataactc gtgcgatgaa ctacttacaa cgggaatcta gtttagagga aattgttcgt
    14581 cttgttggaa ttgattctct gtctgataat gaacgactaa cgatggaaat tgctaaacaa
    14641 attcgagaag attatttgca acagaacgct tttgattcgg tagatacatt cacttcgttt
    14701 gcaaaacaag aagcaatgct aagtaatatt ctcacttttg ctgatcaggc aaatcatgct
    14761 ttagagttgg gttcttactt tacagagatt atggaaggta ccgtggcagt tcgagaccgt
    14821 atggcgagaa gtaaatatgt ttcagaagat agattagatg aaatcaaaat tatatcaaat
    14881 gagattacac atcaaattca tttgatatta gaaacaggag gtctataaat gagtgttata
    14941 aaagaataca gaactgctag tgaagttgtt gggcctctta tgattgttga acaagtaaat
    15001 aatgtgtctt acaatgagtt agttgaaatt caacttcata atggagaaat tcgtcgtgga
    15061 caagttttag agatccacga agataaagca atggttcagc tttttgaagg atctagtgga
    15121 ataaatttag aaaagtctaa aattcgtttt gctggtcatg cattagaatt ggctgtatct
    15181 gaggatatgg ttggtcgtat ttttaatggg atgggaaaac caattgatgg tggaccagat
    15241 ttaattccag agaaatattt agatattgat ggtcaagcta ttaatcctgt atctagagat
    15301 tatccagatg aatttattca gacagggatc tcctctattg atcatttgaa tactcttgta
    15361 cgtggtcaaa aattaccagt attttcaggt tcgggcttac ctcataatga attagctgct
    15421 cagatagcaa gacaagcgac tgttttaaat tctgatgaaa attttgcggt tgtatttgca
    15481 gcaatgggta ttacttttga agaagctgag ttttttatgg aagaactcag aaaaacagga
    15541 gcgatcgatc gttcggtttt atttatgaac ttggcaaatg atcctgcaat tgagcgtatt
    15601 gcaactcccc gcattgcttt aactgcggca gagtatctag cttttgaaaa agatatgcac
    15661 gttctagtta tcatgacgga tatgactaac tattgtgaag cgttacgtga agtctcggca
    15721 gctcgccgtg aagttccagg gagacgaggc tatccgggat atttatatac aaatttatca
    15781 actctatacg aaagggctgg tcgcttagtt ggtaaaaaag gttcggtgac acagattcct
    15841 attttaacaa tgccagaaga tgacataaca catccaattc ctgatttaac tggatacatt
    15901 actgaagggc aaattatttt gtcgcatgag ttgtataatc aaggttatcg tccaccaatc
    15961 aatgttttac cttctctctc tcgattaaaa gataagggat ctggagaagg taaaactcgt
    16021 ggagatcatg ctccaactat gaatcaactg tttgcagcct atgcccaagg gaaaaaggtt
    16081 gaagagttag cagtagtatt aggagaatcg gctttatctg atgtagataa attgtatgtg
    16141 aggtttacaa agcgttttga agaagagtac ataaaccaag gattttataa aaatcgaaat
    16201 atagaagata cgttgaatct tgggtgggaa ttactatcaa ttcttcctag aacagagtta
    16261 aaacgtatca aagatgattt gcttgataaa tacttacctt tggtagaagt ttaatccgga
    16321 aatggagtga ttatctatgg tacgtttgaa tgtaaaacca actcgtatgg aattgaataa
    16381 cttaaaggaa cgtttgacaa cagctgaacg tggacataag ttattaaagg ataaaagaga
    16441 tgaattgatg aggcgattta tttctttgat tcgtgagaat aatcaacttc ggaaagaagt
    16501 ggaaagttat ctaattgata atctaaaatc ctttgcagtt gctaaatcat taaagaattc
    16561 tcaaatggtg gaggaattat tttcaattcc atcgaaagaa attgaattat ttgttgagaa
    16621 agaaaatatc atgagtgtaa cagttcctag aatgcatatg aatattactt ctcaaaatga
    16681 gaacagtgaa tacagctatt tatcttctaa tagtgaaatg gatgatgtat ttgctacaat
    16741 gaatagttta atttataaat tactaagact ggcagaagtt gaaaaaacgt gtcagttaat
    16801 ggctgatgaa atagaaaaaa cacgtagacg tgtaaatggt ttagaatact cgattattcc
    16861 aaacttgtcg gaaactattc attatataga attgaaacta gaggaggcag aaagagccaa
    16921 tttagttcgt attatgaaag tgaagtagat cctttattta gattattaat tagatgaaca
    16981 aatatcagct tggataaggc tttaagcctt tctaagcttt tt
//
LOCUS       DBEJAQ010000041        17003 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.041, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000041 DBEJAQ010000000
VERSION     DBEJAQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.041"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="ACL297_001558"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="ACL297_001558"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGR0071803.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="ACL297_001559"
     CDS             1377..1886
                     /locus_tag="ACL297_001559"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0071804.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4365
                     /gene="pheT"
                     /locus_tag="ACL297_001560"
     CDS             1963..4365
                     /gene="pheT"
                     /locus_tag="ACL297_001560"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGR0071805.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4433..5434)
                     /locus_tag="ACL297_001561"
     CDS             complement(4433..5434)
                     /locus_tag="ACL297_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGR0071806.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5590..6036
                     /locus_tag="ACL297_001562"
                     /pseudo
     CDS             5590..6036
                     /locus_tag="ACL297_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6162..6428
                     /locus_tag="ACL297_001563"
     CDS             6162..6428
                     /locus_tag="ACL297_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0071807.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6689..8938
                     /gene="metE"
                     /locus_tag="ACL297_001564"
     CDS             6689..8938
                     /gene="metE"
                     /locus_tag="ACL297_001564"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGR0071808.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDATFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNSITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9002..9868
                     /gene="metF"
                     /locus_tag="ACL297_001565"
     CDS             9002..9868
                     /gene="metF"
                     /locus_tag="ACL297_001565"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HGR0071809.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10553..12766
                     /gene="pnp"
                     /locus_tag="ACL297_001566"
     CDS             10553..12766
                     /gene="pnp"
                     /locus_tag="ACL297_001566"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGR0071810.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDLEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            12782..13399
                     /gene="cysE"
                     /locus_tag="ACL297_001567"
     CDS             12782..13399
                     /gene="cysE"
                     /locus_tag="ACL297_001567"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGR0071811.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13411..14295
                     /locus_tag="ACL297_001568"
     CDS             13411..14295
                     /locus_tag="ACL297_001568"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0071812.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14382..15725
                     /gene="cysS"
                     /locus_tag="ACL297_001569"
     CDS             14382..15725
                     /gene="cysS"
                     /locus_tag="ACL297_001569"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGR0071813.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15718..16104
                     /locus_tag="ACL297_001570"
     CDS             15718..16104
                     /locus_tag="ACL297_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HGR0071814.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16108..16992
                     /locus_tag="ACL297_001571"
     CDS             16108..16992
                     /locus_tag="ACL297_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0071815.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         5050 a         3321 c         3891 g         4741 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagtg gtaattgaat agaaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctgac tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga cagctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaagt gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggagccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggtt ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataatcgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag aggattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttat atcttataaa
     1981 tggttaaaag aattggtgga cattgatgtg ccatcacaag agttggctga aaaaatgtca
     2041 actacaggaa tcgaggtaga gggtgtcgaa tcaccagctg ctggtctctc aaaaattgtc
     2101 gtcggtgagg tcttgtcttg cgaagatgtg ccagagactc acctccatgt ttgtcaggtt
     2161 aacgttggcg aagaagagcg tcagatcgtt tgtggtgccc caaatgtgcg tgctgggatc
     2221 aaggtcatgg tggctcttcc aggagctcgt atcgctgata actacaaaat caaaaaagga
     2281 aaaatccgtg gtttggagtc acttggaatg atctgttcac ttggtgaatt gggaatttct
     2341 gactcagttg tgcctaagga attcgcagat ggcatccaaa tcttgcctga agatgccgtg
     2401 ccaggtgagg aagtcttttc ttacctagac ttggatgatg aaatcatcga actttccatc
     2461 acaccaaacc gtgcagatgc cctttctatg tgtggagtgg ctcacgaagt ggcagccatc
     2521 tatgacaagg cagtcaactt taaagaattt actctaacag aaactaatga agctgcggca
     2581 gatgcccttt ctgtcagcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     2641 gacaatgtga ccatcgcacc aagtccacaa tggttgcaaa atcttctcat gaacgaagga
     2701 atccgtccca tcaataacgt agtggacgtg accaactaca tcctgctcta ttttggtcaa
     2761 ccaatgcatg cctttgactt ggataacttt gaagggactg acatccgtgt gcgtgaagcg
     2821 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     2881 ctagtcatca ctgtcgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     2941 acagaaatct ctgaaaaatc tagtcgtgtt gtccttgaag ctgctgtttt caatggcaaa
     3001 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     3061 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     3121 gaacttgcag gtgcgacggt gcgtaagggc atcgtttcag cgggtgagct tgatacttca
     3181 gatgtagaag tttcttcaac ccttgctgat gttaatcgtg tcctcggaac tgagctgtct
     3241 tatgctgatg tagaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     3301 agctttacag tcagagtccc acgtcgtcgt tgggatatca caatcgaagc tgacctcttt
     3361 gaagaaattg ctcgtatcta tggttatgac cgcttgccaa ctagtctacc aaaagacgat
     3421 ggtacagcag gtgaattgac agccacacaa aaactccgcc gtcaagttcg tactattgct
     3481 gaaggagcag gtttgacaga aatcatcacc tatactctaa caactcctga aaaagcagtt
     3541 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     3601 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     3661 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     3721 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     3781 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taagggaatc
     3841 cttgaagccc tatttactcg tttgggactc caagtaacct atacagcaac atctgaaatc
     3901 gctagccttc atccaggtcg tacagccgtg atttcattcg gtgaccaagt tcttggtttc
     3961 cttggccaag tgcatccagt cactgccaag gcttacgata ttccagaaac gtatgtggct
     4021 gagcttaacc tttcagctat cgaagctgcg cttcagccag cgactccatt tgtagaaatc
     4081 accaaattcc cggcagtcag ccgtgacgtt gcccttctcc tcaaggcaga agtgactcat
     4141 caagaagttg tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     4201 tttgacgtct tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     4261 accttccaaa atccagaaga tagcttaacg gacgaagaag tcgcacgcta tatggaaaaa
     4321 atccaagcat cgctcgaaga aaaagtcaat gcagaagtgc gttaaacgat agtatgatac
     4381 aaattccccc tctagtttat gaacactaga ggggtgattt tatataaaat aattaaaatg
     4441 tgatttctgt aaaaatacat ctatgatcag agttttgaaa acttacaatg tctaaatctt
     4501 taacatagta gtggttttta ggcaataaaa tatgatctat tgttgcatta aaaagttttg
     4561 gactttggct attccaagtt cctctttcaa aaggtggcag tacatttaat gcgtccctat
     4621 gagagcttat ttttgcaagt gctccatgac gcatagttgc attaaaatca cctgcaataa
     4681 tagcctttgg atattttgaa gccaattgat tatgaatgat gtttaagtct tgcttccaga
     4741 tttccattaa acctggcaga ggaggagcag tatgcaaggc aatgatatct ggtatatttt
     4801 gttttctcga atgtaataca attgtcccga accgtgttgt atgaaaagtt ttagcttctg
     4861 tatagaaacc ataacttttc ttgacaatca cagtcacagg agctattcca ctattggtag
     4921 gtggagaagt gaaaatatca tagttggcca tagaaagtcc aacttgatga aacaatagtt
     4981 tgattctctg gttttcttgc tcacctctga tattggtagc tagttcaggg aatatagcca
     5041 tatcggcgtc aaaatggcta aaaattcgct caatatgttg tgcttctatt tgattagcga
     5101 cgttccaagt tactaatttt aaggttttag tcttatttga tgcagaagaa agggttttat
     5161 ctgttccgaa taatagtaat atgagcgata gtaagagcaa aatgccagag taaaaccgta
     5221 tttgtttatt tttcttgtaa aaagataggg aaaaaaatac gaaacttaaa atagcagcca
     5281 tagttgcagt agctaagggg aaggcataaa tctgtgccaa gataggttgg gtagatagcc
     5341 aaatgttaag attagaagat gggaaaaaag aataagttat aatagggata gaaaagagaa
     5401 tgaccgaaag ggcagagaga aatgttttaa tcattgtgtg agtacctcaa tttgcaatgg
     5461 ttgctcggag ctaatgtgac tttgtgactt tacttgattg atattctttt tcttgtcttc
     5521 ctcatagatt aagtacaacc caagtaaaaa aagtccaatt taagattgaa atgattaaaa
     5581 ggcacagaaa tgcaagacaa ttatactaca aaaggcaaac atttgacaat cgatagccgt
     5641 cgcttaatcg aacgatggaa aaaagaagga aaatcaaata gagaaattgc ctctctactt
     5701 ggaaaagctc ctcaaactat ccatactgaa atcaagtatg ggacagtccg acaatgtctt
     5761 ggaaaagggc gcttcaaaga gatttattct gccgactacg ctcaacagtc ttatgaaaat
     5821 aatcgcaagc actcggtcaa gaaatcaagc ttgactaaga aactaaagga aaagattctc
     5881 cactatcata accaaaaatt ttcgcctgaa atgatggtga tggctaaagg ggttaacgtg
     5941 ggaatttcaa ccatttacta ttggattcat cgtggaaaat tggggttaag caagcaggat
     6001 ctgctttatc ctagaaaagg aaaagcgctt aagaaatagg ctagcaccaa ctttaaaccc
     6061 gctggtcaat ctatcgaatc ataattcgct cattttttta aaaatacata gacgtaaaaa
     6121 aaagagggtt aagtgatgaa gaatagtgct attgggagta attggaagga tgtccatctg
     6181 agctctttag caaggaggaa atccttgaaa gtgatatgcg agtggctatc atgagcgagt
     6241 tgattgaggc taggtatgaa caaggaatca gtcagaaaaa gctggaagaa gtcagtggaa
     6301 taagccagcc tgtcatagct aggatggaga caggagagag cagtcttcag ttggatacgg
     6361 tcttaaaagt tctaaccagt ctaggaaaga cactagcagt cgttccactt gaacagggga
     6421 aaagttgact agaatcagtt atagtctttt ctaataacag gtcatagtca cttataagaa
     6481 ttactaataa catgttttag tattctaatt ggaatatagc tgtaaagtaa gaattacaaa
     6541 ggatttgaga cgtttgcttc aagtcttttt cttttgtccg aaacagatat atagtttcaa
     6601 actatatcaa accctaattt tttacaatta tacagacgct ttcttttctg ttaagatagt
     6661 ttcaacaaca aattttggag gacacatcat gtcaactaca atcatcggtt tccctcgttt
     6721 gggcgaattc cgtgaattaa aatttacaac tgaaaaatac tttagaaaag aaatctcaga
     6781 agaagaactt ttagcagcag caaaagactt gcgtgctaaa cactggaaca ttgtcaaaga
     6841 aaaaggcatc actgaaattc catcaaatga cttttctcac tatgataact tcctagatgc
     6901 gactttcctt ttcaacgtgg tgcctgcatc cgttcaaaac ttggacttgt ctgaccttga
     6961 gcgctacttc gctttgggac gtggttacca gggagaaaaa ggggacgttc gcgcccttcc
     7021 aatgaagaaa tggttcaaca ctaactacca ctacatcgtt cctaaatttg aaaaagacac
     7081 tcaagtaaaa ttggctggtc acaagatttt cgatgagttc caagaagcaa aagaacttgg
     7141 attgaacact cgtcctgttc ttgtaggtcc attcactttc cttcaattgt cagactttga
     7201 agaaggcgtg aaagcagacg acttcgtaga tagcttagtg gttgcttacc aagaagtttt
     7261 tgctaaattg gctgaacttg gtgcgactcg catccaattg gatgaagcgg ctcttgtaaa
     7321 agacttgaca gctgaagaaa aagctctctt cttgaacctt tacaacaaac tcttggctga
     7381 caaaaaaggt cttgaagtct tgcttcaaac ttatttcggt gatgttcgtg acgtatacgc
     7441 tgaccttgta aacttgccag tagatgctat cggtcttgac tttgttgaag gtaagaaaac
     7501 tcttgaactc gttaaaggtg gcttcccagc tgacaagact ctctatgtag gtattgtcaa
     7561 tggtaaaaac atctggcgca acaactacga aaagagcttg gctgttcttg agcaaatccc
     7621 agctgaaaac atcgttttga caagctcttg ctcacttctt catgtgccat ttacaactgc
     7681 taatgaagaa tttgaaccag cactcttgaa ccactttgcc tttgcagttg aaaaattgga
     7741 tgaaatccgt gacttggatg ctatccgcaa tggtcaaggt tcagaagctc ttgcagccaa
     7801 caaagaactc tttgcgactg agcgtgttgg tgaaaatgct gaacttcgtg cgcgtatcgc
     7861 tggcttgaca gacgcagact acactcgttt gccagccttt gcagaacgtg aagctatcca
     7921 agaagaagct ttcaaacttc cagctcttcc aacaacaacg attggttcat tccctcaaac
     7981 aaaagaagtt cgtgccaaac gtttggctta ccgtaaaggt gaattgtctc aaaaagagta
     8041 cgatgctttc cttgctgaaa cgattgacga atggatcaag tggcaagaag atattgactt
     8101 tgatgtcctt gttcacggtg aatttgagcg taatgacatg gttgagtact tcggtcaaaa
     8161 cttgtcagga tacctcttct ctaaaaatgg atgggtacaa tcatacggta tgcgtggggt
     8221 aaaaccacca atcatctggg gtgatgtcac tcgtcttaac tctatcactg ttaaatggtc
     8281 tagctatgca caaagtcgta caaacaaacc tgttaaaggt atgttgactg gacctgttac
     8341 catccttaac tggtcattcc cacgtgaaga catctctatc aaggattcaa ctcttcaaat
     8401 cgcccttgct atcaaggatg aagtacttga ccttgaagct gctggtgtga aaatcatcca
     8461 aatcgacgag gctgctcttc gtgaaaaatt gccactccgc cgtagcgact ggtacgaaga
     8521 ctaccttgac tgggctattc ctgccttccg cttggtacac tcaacagtag cgccagacac
     8581 acaaatccac actcacatgt gttactcaga atttacagat atcatcccag ctatcgataa
     8641 catggatgca gacgttattt cctttgaagc tagccgttca aaccttgaaa tcttggacga
     8701 actcaaagcg aaaaacttcc aaacagaagt gggacctggg gtttacgata tccactcacc
     8761 tcgtgtacca aatgaaggcg aaatcgacaa cacaatcgaa gccatccttg ctaaagtgcc
     8821 aagcaagaaa gtttggatca accctgactg tggtttgaaa acacgtggta tcccagaaac
     8881 aaaagaaagc ttgatccgcc ttgttgaagc agctaaagct gcgcgtgaga aattgtaaga
     8941 ttggatttct ctccatacag tggttgttca aaaagaaatc aactagaaaa ggttattaca
     9001 tatgtcacgc caaacaccgt cactctcatt tgaagtgttc cctccaaacc cagccgtggg
     9061 taatggtaac attatttcag ccttgcagga tatgcaggag ttggctcccc attttatcag
     9121 tgtaactgcc agcaataata aatttaatat caaggaaacg acggttcgtt tggctgactt
     9181 tatccaaaat gacttggcga ttccgaccat tgcccacttg ccagctattt atctaactaa
     9241 ggacaaggtt gctgaaacta ttgctgactt ggacaaagtt ggggtgcaga aaatcttggc
     9301 tcttcgtggg gatattattc cagacgtaga accacaaaag gatttccgct atgcaaccga
     9361 cttgattgag tttatcaagg aacaagcccc tcactttgat attattggag cttgctatcc
     9421 agaagggcat ccagattcgc caaatcagat ttcagatatt caaaatctta agaagaaagt
     9481 ggatgcaggc tgttcgagcc tcgtaactca gctcttcttt gataatgagc gcttctatga
     9541 ttttcaggac aagtgtattt tggctgggat tgatgttccc attcatgcag gaattatgcc
     9601 aattctgaat cgaaatcagg ctctccgact cttgaagact tgtgagaata tccatcttcc
     9661 acgcaaattt aaagccatct tagacaagta tgagcatgac cctgagtcgc tcagagcagc
     9721 aggacttgcc tatgcagtgg accaaatcgt ggacttggta actcaggatg ttgccggtgt
     9781 gcatctctat acgatgaata atgctgatac agcaaaatac atccatcaag caacccatgc
     9841 cttgtttaat caccagtctc taggataata aaagcaaacc attcttctca ggtgagggga
     9901 atggttcctt tttaatggta aagacctcac tttttaagaa aaatatgata aaataaattc
     9961 tgtacgtact tgatacaaag atgaaggtat taaataatac aatgcattta aactgtaaga
    10021 tttaacgaca cgagtgagtc aaatcgatgt tatttgacga gagagttagt aaagcattta
    10081 gctaatcgcc cgatagcgat tagcgtgaaa gctttagata ctttagtatt aaacctttct
    10141 aatgatgtat cttgaaaact gaacttgcct acaactctgt ggaaaaagat aaatctccct
    10201 aggagcagtc gctccgtcgt tcgatttcct attttccttt gagttgcttg acggcttggt
    10261 atcttgatga cttgaatttt ggtctcattt cttaggaaaa aagataagct tcctagcact
    10321 cgtcgagtgc gacgtcacct tcctattttt ctacagaaat gttcggcaag ccgaaccgtc
    10381 caaaatatct tgtgtaattg aacacggccg aaaagctgtg taaaaaagat aaactgtctt
    10441 gtcttcatcg aagacttcgt cagtttccta tttttacttt gcttttgacg tccttggtat
    10501 cttgatcttt gtaggcaagg cgtataattt catcaatcca aaggggatta aaatggcaaa
    10561 acaagtgttt caaacgactt ttgcgggtcg tgagttaatt gtagaaactg gtcaggttgc
    10621 taagcaagca aatggctctg ttgtcgtacg ttacggtgag tcaactgtct tgactgctgc
    10681 cgttatgtct aagaaaatgg caactgggga tttcttccca ctccaagtca actacgaaga
    10741 aaaaatgtat gcggctggga agtttcctgg tggctttatg aaacgtgaag gacgtccttc
    10801 aacagatgcg accttgacag cgcgtttgat tgaccgtccg attcgtccta tgtttgcgga
    10861 aggtttccgt aatgaagtcc aagtcatcaa tacagtgctt tcttatgatg aaaatgcgtc
    10921 tgcaccaatg gctgctatgt ttggttcatc tttggcactg tctatttcag atattccatt
    10981 tgacggacca attgctgggg tacaagtggg atatgtagat ggccaaatca tcatcaaccc
    11041 aagtcaagaa caagcagagc aatctcttct tgaattgaca gtagctggaa ccaagcacgc
    11101 tatcaacatg gtagagtctg gtgctaaaga attgtcagaa gaaatcatgt tggaagcgct
    11161 ccttaaaggg cacgaagctg tcaaagaatt gattgccttc caagaagaaa tcgttgctgc
    11221 tgtcggtaaa gaaaaagcag aagtggaatt gcttcacgtg gatgctgaat tgcaagctga
    11281 aatcattgca gcctacaaca gtgacctcca aaaggcagtt caagtagaag agaaattggc
    11341 ccgtgaagct gcgactcaag tagtgaaaga ccaagtgact gccgtttacg aagaaaaata
    11401 tgcggaccac gaagaatttg accgtattat gcgtgatgtg gctgaaatct tggaacaaat
    11461 ggaacacgca gaagtgcgac gtttaattac agaagacaag gtgcgtcctg atggtcgtaa
    11521 ggtcgatgaa atccgtcctt tggatgcggt tgtcgacttc cttcctcgtg tgcacggttc
    11581 aggtctcttc actcgtggac aaacgcaagc cctttctgtc ttgaccttgg ctccgatggg
    11641 agaaactcaa atcattgatg gtttggatct ggagtacaag aaacgcttta tgcaccacta
    11701 taacttccct caatattctg taggggaaac aggtcgttac ggtgcgccag gtcgtcgtga
    11761 aatcggtcac ggtgcccttg gtgagcgtgc tcttgctcaa gtcttgccaa gcttggaaga
    11821 attcccatac gctatccgtc tagtagcaga agttttggaa tcaaacggtt cttcatctca
    11881 agcttctatc tgtgcgggaa ctcttgccct tatggctggt ggtgtgccaa tcaaggcgcc
    11941 agtagctggt attgctatgg gacttatctc agatggaaat aactacacag tattgacaga
    12001 tatccaaggt ttggaagatc actttggaga tatggacttc aaggttgcag gtactcgtga
    12061 tgggattaca gcccttcaaa tggatatcaa gattcaaggg attactgcag aaatcttgac
    12121 ggaggctctt gctcaagcca agaaagcgcg ttttgaaatc cttgatgtca ttgaagcaac
    12181 cattccagaa gttcgtccag aattggctcc aactgctccg aaaattgata cgatcaagat
    12241 tgatgtggac aagattaaga ttgtcatcgg taagggtgga gaaaccatcg acaagattat
    12301 cgctgaaaca ggtgttaaga ttgatataga cgaagaagga aatgtgtcta tctactctag
    12361 tgaccaagat gctattaacc gtgccaaaga aattattgct ggtttggttc gtgaagccaa
    12421 agtggatgaa gtttaccgtg ctaaagtcgt tcgtatcgag aaatttggtg cctttgttaa
    12481 cctctttgat aagacagatg cccttgttca tatctctgag atggcttgga ctcgtaccaa
    12541 tcgtgtagag gatttggtag aaatcgggga tgaagttgat gttaaggtta tcaaaattga
    12601 tgaaaaaggc cgtatcgatg cctctatgaa ggctcttcta cctcgtccgc caaaacctga
    12661 gcatgatgaa aaaggtgaaa agtctgagcg ccctcaccgc ccacgtcatc aaaaggatca
    12721 caaacctaag aaagaattta cagaaacacc aaaagattca gaataagaaa aggagaaatg
    12781 tatggggtgg tggcgcgaaa ccattgatat tgtaaaagaa aatgatccag cggcccgcac
    12841 cactttggag gttttgctga cttatccagg tgtcaaggcc ttggcggccc accgtctctc
    12901 gcattttctc tggaagcacg gcttcaaact attagctcgt atgtacagtc agttttggcg
    12961 cttttggact cagattgaga ttcatccagg agcccagatt gattcaggtg tttttattga
    13021 ccatggttct ggtctggtga ttggagagac agcgattgtt gaaaaaggcg ttcttctcta
    13081 tcacggagtg actctcgggg gaacagggaa agactgtggc aaacgccatc cgactgtacg
    13141 aaagggagcc ctcatatcag cccatgccca agttatcggg cctgtggaaa ttggtgaaaa
    13201 tgccaaggtc ggtgctgcag cagttgtcgt agcagatgta cctagtgatg tgacggttgt
    13261 cggtattccg gccaagattg tccgtcttca tggtaagaaa gatgagcctg ttattcacga
    13321 agtcgaagaa aaacgggagt attatgtcaa taaactcgag caggctaaag atgccagtca
    13381 cagatcgtct ggtttgtaga ggatacctat atgattcaag caagaaacaa gttaagccaa
    13441 gaggagctat ctgaggcgaa aaaagtaatt aactgttgcc aaaactatga cggtacctat
    13501 cgcgatccct atctctctaa catgcttaat tttgacccaa acatgcccgc ctttttcctt
    13561 tattatgaaa aaggcaaact tgttggttta ttaactgtct atgcagatga ccaagatgta
    13621 gaagtgacga tattggttca tccaggtcat cgccgtcagg ggattgcgcg tgcattgttt
    13681 actagttttg agagagaaac agcttctttc cccattcggt cagtcacttt tcagacagaa
    13741 cgtatttttt tagagaatca tcctgacttt gccagtaact ggggactaat cgaggatgaa
    13801 gagacagaaa cttggttagg taaggataga agaccttatc cgttagcaaa tgtttcccat
    13861 cttgaagttt tgttagcaga tagttcgtat caggatcaaa ttagtcaatt aaaatttcag
    13921 gcattttcag aggaacatga atcgagagaa gttgtggata gatatgtcgc tgaagctctg
    13981 aaggatccag aaagtcgctt atatattttg ttaaaagacg gtcaggttat tgggacttgc
    14041 acggtagatt tatcgactaa tacgaattac ttctacggtt tagcaatatc ggaacctgaa
    14101 cgtgggaaag gctatggaag ctacttagca aaatccctcg tcaaccaact aattgagcaa
    14161 aatgacaagg aatttcagat tgcagtggaa gatagcaatg taggtgccaa acgtttgtat
    14221 gaaaaaattg gctttgtcaa acagacacag gtggtttatc tgaatgagaa aggagcaagg
    14281 gattccgaag tgtagagaca ttcggactga aatttacttg agctcttagt ttagtgatga
    14341 aactaacagt tcttggattt tagctttcct gattatgatt tatgattaaa atctatgaca
    14401 ccatgtctcg tgatttgcga gaatttgtcc cgattgagga cggcaagatc aagatgtatg
    14461 tttgtgggcc aaccgtgtac aactatattc acgtgggaaa cgcccgttcg acggtagctt
    14521 ttgatacgat tcgtcgctat tttgagtacc gtgggtataa ggttgcctat atttccaatt
    14581 ttacagatgt ggatgataag attatcaacc gtgccaggga agaaggcatc acacctcagg
    14641 aggttgcgga taagtacatc gctgcctttc gtgaggatgt gacggcctta ggcgtgaaac
    14701 ctgcgactcg ccatccgcgt gtagtggagt ttatggcaga catcatccgt tttgtggaag
    14761 acttgatcga gaaaggcttt gcctatgaga gtcaagggga tgtctatttc cgtgtagaaa
    14821 aatcccacaa ctatgctaaa ttggctaata aaaccttgga agatttggag ctaggtgctt
    14881 caggtcgtac cgatgaagaa acggctcgta aggaaaatcc tgtagacttt gctctatgga
    14941 aatcttccaa accaggtgag atttcttggg atagtccttg gggacctgga cgtccgggtt
    15001 ggcatattga gtgttcagtc atgtcgacag agattttagg tgataccatt gatatccacg
    15061 gtggtggagc tgacctagag tttccacacc acaccaatga aattgcccaa tctgaagcta
    15121 aaacaggcaa ggcttttgct aactactgga tgcacaatgg ctttgtcaat atcgacaatg
    15181 tcaaaatgtc taagtccttg ggtaacttca ttacggtaca cgatgccctt aaaactcttg
    15241 atgggcaagt acttcgtttc ttctttgcga cccagcacta ccgtaagcct atcaacttca
    15301 cggaaaaggc agttcgcgat gccgagacca atctcaagta tctgaagaat acttacgaac
    15361 aaccatttac aggaaacgtg gatgcccaag agttacaaaa ctttaaagat aagtttgtag
    15421 cagctatgga tgaagatttt aatgctgcca acggtatcac agttgtcttt gaaatggcca
    15481 aatggatcaa ctcagggaac tatgatgcaa gtgtcaagca agctcttgca gatatgttag
    15541 aaatttttgg aattgtcttt gttgaggaag ttttggatgc agagattgaa gacttgattc
    15601 aaaaacgcca agaggcgcgt gccaatcgtg actttgagac agcagaccaa atccgtgacc
    15661 aattggttac tcaaggaatt aagctccttg ataccaagga tggagtgagg tggacacgtg
    15721 attgatgtca atctcattaa cgggattgcg ctagcctttg agggagatgc ggtgtattct
    15781 atgtatattc gccgccacct catcctcaaa ggtatgacca agcccaataa actccaccaa
    15841 gaggcgacca agtacgtgtc agccaaggct caggctcgtc tgattgctct catgttggag
    15901 gagcagatcc taacggaaaa agaagaagaa atctacaaac gtggccgcaa taccaatagt
    15961 cacacaaagg ctaaaaatgc agatgtcgtg acttatcgta tgtccacggg atttgaagca
    16021 gttatgggct atctccatat gactgagaat ctggaacgtc ttgagagttt ggtttcatgg
    16081 tgcatccaaa aagtggaggg ctagaacatg agggcaaaag aactacaaga ctggtttcct
    16141 gaggctcgga tttcagacca accagtagag aaagagggct atctcacgct tcctttagct
    16201 tctcagcagt ggattttgct ggaggaagct gggctcagcg agcgtgaaaa gcagttggtt
    16261 gcccttttga cccagcagga gcaggctcgt tcgctaaacc cttggtattc ctatctggtt
    16321 gagggcaagg gacaggcacc gcaagttttt aaaaagattc agttggttta ttgccatctt
    16381 tcttattttc agcaggaaaa tctgacttct tggctagata tgatgcggac tctttttccg
    16441 aattgtcaga cagtgctaca ggtcggagct caggattatg ttttcgtgct tcaacaagac
    16501 aaatacactt ctgtaagaga tattttgagt gatacgattg aagcggttga gtatgacttt
    16561 ggacttcgtc tttctatcat gttgggtcag gtttggtctc agacaggaca tcaagcccta
    16621 tcagacttaa tcaaagctga gcgggatttg ttcaagacat ggtggcgtca gggtcaccaa
    16681 ggtgttcata ctttttctca gctctatctt tggagtatgg gagaaagact cgtggacttg
    16741 aagccaatta aagaatgtct acaccagatg attttggatc aagatcagat tcaggaaatc
    16801 attctctctc tttgggaaaa tagtgctgtt ctcactaaaa cagcccagca actctatctg
    16861 caccgcaatt ctctccaata caagattgat aaatgggaag agttgacagg gcttcagttg
    16921 aaagagttga ccgacctgac cttgtgttat caattgattt taggttcttt gtcaactata
    16981 gttggtttgt aaagaagtta ata
//
LOCUS       DBEJAQ010000042        16183 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.042, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000042 DBEJAQ010000000
VERSION     DBEJAQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.042"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            145..6846
                     /gene="bgaA"
                     /locus_tag="ACL297_001572"
     CDS             145..6846
                     /gene="bgaA"
                     /locus_tag="ACL297_001572"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adhesin/beta-galactosidase BgaA"
                     /protein_id="HGR0071816.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPDTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7094..7540)
                     /locus_tag="ACL297_001573"
     CDS             complement(7094..7540)
                     /locus_tag="ACL297_001573"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGR0071817.1"
                     /translation="MFDKEQKRGYKQSAKNLANWHYNDKEDSYIHPDGWCYRFHHIKY
                     QKTQTDFQQEIKVYYADEPESAPQKGLYMNERYQNLKAKECQALLSPQDRQIFAQRKI
                     DVEPVFGQIKACLGYKRCNLRGKRQVRIDMGLVLMANNLLKHSEMK"
     gene            complement(7705..7950)
                     /locus_tag="ACL297_001574"
     CDS             complement(7705..7950)
                     /locus_tag="ACL297_001574"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGR0071818.1"
                     /translation="MHIHYNTNQTTLPLEISSFLPQDHLVFTIEKVVNTLEERHFYTS
                     YHAFGRPSYHPKMLVSTLLFAYSQGIFSGRKIEKWKS"
     gene            complement(8161..8520)
                     /locus_tag="ACL297_001575"
     CDS             complement(8161..8520)
                     /locus_tag="ACL297_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HGR0071819.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            8612..9568
                     /locus_tag="ACL297_001576"
                     /pseudo
     CDS             8612..9568
                     /locus_tag="ACL297_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9552..10109
                     /locus_tag="ACL297_001577"
     CDS             9552..10109
                     /locus_tag="ACL297_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGR0071820.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10102..10356
                     /locus_tag="ACL297_001578"
     CDS             10102..10356
                     /locus_tag="ACL297_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071821.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10368..12422
                     /locus_tag="ACL297_001579"
     CDS             10368..12422
                     /locus_tag="ACL297_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGR0071822.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12601..13461
                     /locus_tag="ACL297_001580"
     CDS             12601..13461
                     /locus_tag="ACL297_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HGR0071823.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            13672..14382
                     /gene="ccdA2"
                     /locus_tag="ACL297_001581"
     CDS             13672..14382
                     /gene="ccdA2"
                     /locus_tag="ACL297_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGR0071824.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14402..14968
                     /locus_tag="ACL297_001582"
     CDS             14402..14968
                     /locus_tag="ACL297_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HGR0071825.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14979..16091
                     /gene="msrB"
                     /locus_tag="ACL297_001583"
     CDS             14979..16091
                     /gene="msrB"
                     /locus_tag="ACL297_001583"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGR0071826.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         4909 a         3067 c         3577 g         4630 t
ORIGIN      
        1 catatctacg gcaaggcgac gctgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa atgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggca ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 aatttcgatc atgaatctcc tgcacaaaat gaaggtggac aactcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc tatccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac gtggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggcaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcatcagc aacccgtaca
     2101 cgaggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt cagattatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccagat accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc acccttactg ctcactctga tctcttgaaa
     3061 tctagtcaag tcactgtctt tactggtaag aaagaaggac aagaaaagac tgttttgggg
     3121 acagaggtgc caaaagtaca gaccattatt ggagaggcac ctgaaatgcc taccactgtt
     3181 ccgtttctat acagtgatgg tagtcgtgca gaacgtcctg taacctggtc ttcagtagat
     3241 gtgagcaagc ctggtattgt aacggtgaaa ggtatggctg acggacgaga agtagaagct
     3301 cgtgtagaag tgattgctct taaatcagag ctaccagttg tgaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcctatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaagca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ttgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcaag catatttgtt cagcctaaag atggtggccc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagctt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagtgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtcca
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag actcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgtcc tattcggaga ttcaggtatt
     4741 ttgagcaaac gttcagttga taacttaagc gttggcttcc atgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtga gagcagacaa caaactagga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa gcaaggacaa
     5101 acaagaatcc aagttgacgg taaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttacg gcagtcacag caagtgttag caacaatggc
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccgg ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaaaac
     5461 ctgacagttg aatgggaaga agttccagcg gaaaatctga taaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgct
     5821 caagccaaac ttcacttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagcagat aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac caagtgaatt gcctcaagaa agcacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgaga agacagttgc tgctgtacaa
     6421 gaagatcttc caaaactcga atttgttgaa aaagatttgg cctacaagac agttgagaaa
     6481 aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa aactggtaaa
     6541 gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg
     6601 gtagaagctc cgacagaccg catcgtcttg gttggaacca aaccagtagc tcaagaagct
     6661 aaaaaaccac aagtgtcaga aaaagcagat acaaaaccaa ttgattcaag tgaagctagt
     6721 caaactaata aagcccagtt accaaataca ggtagtgcgg caagccaagc agcagtagca
     6781 gcaggtttag ctcttctagg tttgagtgca ggattagtag ttactaaagg taaaaaagaa
     6841 gactagaaat tttgatacct tctttatcat taaagaccaa agaaaaactc ttgtcctaaa
     6901 tagaagataa agagtctgga acaaaatagt tctcaaccac aaaaaagcta gatatttcca
     6961 attgcggaac tctagctttt taattttgag tcgttctctt attgtatagt agattgaaac
     7021 tagagtagta cacttctact tctaaaacat tgttagaaat cgatttgact gtccttatcg
     7081 atttgtcctg ttcttatttc atttcactat gttttaggag gttattggcc ataagtacca
     7141 atcccatgtc aattctcact tgacgcttgc ctctcagatt acatctcttg taacccaaac
     7201 aagcctttat ctgcccaaag acaggttcta catcaatctt gcgttgagcg aaaatctgtc
     7261 tatcttgggg agataaaagc gcctgacatt ctttagcttt caagttttga tagcgttcgt
     7321 tcatatacaa tcccttttga ggggctgatt caggttcatc agcgtagtaa accttgattt
     7381 cctgttgaaa gtctgtctgt gttttctggt acttgatatg gtgaaaacga tagcaccagc
     7441 catcaggatg tatgtagcta tcctccttgt cattatagtg ccaattcgct aagtttttag
     7501 ctgactgttt ataccctctc ttttgttcct tatcaaacat ggcatattta atcagaaggg
     7561 gagtgatttc ttcttgaaaa tagacttgta tctgttcttg aagtttggcg gagaatttct
     7621 ctgtcgtttt cttccacaca aaactatact tgttggcatt ggattcaatc ttagttccgt
     7681 caataaacaa acaatctaag gtcactaact cttccatttt tcaatttttc gaccagagaa
     7741 aatcccttgt gaataggcaa atagaagagt agatacaagc attttagggt gataagacgg
     7801 gcgaccaaag gcatgatagg aggtgtagaa gtgacgttcc tccaaggtat tcaccacttt
     7861 ttcaatagta aagacgagat ggtcttgtgg taagaaggaa ctgatttcta gtggtaaagt
     7921 tgtttgattt gtgttatagt gaatatgcat gggatagcct ttctaaatgg tttattaagc
     7981 aattctattt taccaagact atcgtaacca tgcaaaaaag caacggcaaa tccgttgcct
     8041 tttttggata taagagacta ttgtcccaga ctcttttctt tgtataaaat tgtatagtgt
     8101 tattgtctat actattttgt agagaaagca aaaatcccta gaaaatgctt agggattcga
     8161 ctatttcatt aagaacaatt caacaatcat ctctatatac atgataaggg ttaataaaaa
     8221 tcctgtacgc cagaagaatt tgatgaattt aggatagtaa aagctgcgtt tttttaaaag
     8281 gaagaaaaag acgagaataa tggctaggaa cgagagggct agccccagtc tagggaggaa
     8341 attatgggta aaggttttag ctgtaatcaa gtaatactca aataccaaga ctggaaaagc
     8401 caaatccgca aagtttcgtc ctagtttttt taatctaaaa agtttggtta ggataatgca
     8461 gacgactaag gtcagtatca ataataaaat agatgctaat ttcattaaaa tcatacccat
     8521 attgtatcat aaaaaaaggc taatggaaac gagaaacaga ggaatttata ggaaagtcag
     8581 gcttttgaga ttgtgggtga tttttgttat aatgaaagtt ataaaatctt atgatacctt
     8641 gaatgattat tatcgaaaac tctttggaga aaagactttt aaagtcccta ttgatgcggg
     8701 atttgactgt cccaatcgtg atggaactgt ggctcatgga ggctgtactt tttgtacggt
     8761 ttccggttct ggagatgcca ttgtagcccc agatccgcct atccgtgagc aattttataa
     8821 ggaaattgac tttattcatc gtaagtggcc agatgttcag aagtatctgg tttattttca
     8881 aaattttacc aacacccatg aaaaggtgga agttattcga gagcgttatg aacaggctat
     8941 caatgagcca ggtgtggtag gaatcaatat tggaacgcgc ccagactgtt taccagacga
     9001 aaccatcgaa tatttggctg agttatcgga gtgcatgcat gtgacggttg aattgggctt
     9061 gcagactact tatgaagcaa cctctgacct gattaaccgt gcgcattctt atgaattgta
     9121 ggtggaaacg gtcaagcgtt tgagaaagta tccaaaaatt gagattgttt cccatttgat
     9181 caatggtttg cctggtgaaa cccatgagat gatggttgaa aatgtccgcc gttgtgtcac
     9241 ggataacgat attcaaggga ttaaactgca tttgctccat cttatgacaa atactcgtat
     9301 gcaacgagat taccatgagg gacgtttaca attgatgagt caggacgaat atgtcagggt
     9361 catctgtgac caactggaaa ttattcccaa acatatcgtc atccatcgaa ttacaggaga
     9421 tgcgcctagg gatatgctga ttggtcctat gtggagcctc aataaatggg aagtcctcaa
     9481 cagcattgag atggagatga gacgtcgtgg aagtgttcaa ggatgcaagg ctgtaaaaca
     9541 ggagtttgaa aatgaaaaga ccacttgaga tggcacatga ttttttggct gaggtcgtga
     9601 caaaagagga tgtcgtagtg gatgcgacta tgggaaatgg tcatgacacg ctttttttag
     9661 ccaagctagc caagcaagtc tatgcctttg atattcagaa gcaagccttg gaaaagaccc
     9721 aagagcgttt gcatcaggct ggcttgacaa atgcccagtt aatcttgcaa ggccatgaga
     9781 cactggacca gtttgtgaca aaagctaagg cagggatttt taatttgggc tatttgccgt
     9841 cagctgataa gtctgtcatc acccgaccgc agacaacgat tgaggcatta gaaaagctat
     9901 gtggcttact tgtcaaaggt ggacgaattg ctattatgat ttactatggt catgaaggag
     9961 gcgacctcga gagagatgct gtcttggatt ttgtgatcca gttgaaccaa caagagtaca
    10021 cagctgccat ttaccgaact ttaaaccaag tcaacaaccc gccgttttta gtgatgattg
    10081 aaaaattaga gagatacaga catggataaa caatacctac acgaaaagct ggatgccatg
    10141 cgccagaatt ttgttgaatc aacctaccac gaacgagcgg tgggcgtgct agaccaagcg
    10201 catatgagca aaaaaatgct taaaatcaag aaaaaattag ttgctcttga aatggaacgg
    10261 tgccagagaa aaattgagca caaagactgt tccaagattg accaaaaaat caaagagcag
    10321 aaggagatat ttgaatcctg ttgtaaaaaa gattaaggag gacgtgcgtg gaattactga
    10381 tttacctcat cctattttta ctggtcttga ttgtctcaag tacaaccaat aagctcctac
    10441 cctttttgcc tctccctttg gtgcagattc ttttgggaat tgtgattggt ctctttttac
    10501 ccaatactga ctttcacctt aatacggagt tgtttttggc actggttatc ggccccttgc
    10561 ttttccgaga ggctgaagaa gcagatgtta cggctatttt aaaacactgg cgaatcattg
    10621 tttatctcat atttccagtg atttttatct cgaccctgag tttgggtggc ttggcccatc
    10681 ttctttggtt cagccttccc ttggcagctt gcttggctgt tggggcagcc cttggtccta
    10741 cggacttggt ggcctttgcc tctctttcgg agcgttttag ctttcctaag cgcgtgtcca
    10801 atattcttaa gggcgaagga ctcttgaatg atgcttctgg tttggtggct tttcaggtag
    10861 ctttgacagc ttggacaact ggagcttttt ctctggggca agctagcagt tcactcatct
    10921 tttcaatcct aggcggtttt ttaattggat ttttaacagc catgaccaac cgcttcctcc
    10981 ataccttctt gctaagtgtg cgtgcaacgg atattgccag tgaactttta ttagaattga
    11041 gtttgcctct tgtgaccttc tttctggcag aagaagttca tgtttcaggt attattgccg
    11101 tcgtagttgc tggaatttta aaggcaagtc gtttcaagaa aatcacgctc ctcgaagccc
    11161 aagtggatac ggtgaccgag acggtctggc atacagtgac ctttatgctc aacggttctg
    11221 tctttgtgat tttagggatg gagctggaaa tgatagcaga acctatcttg accaatccaa
    11281 tctataatcc tctactttta ttgctatctc ttatcgccct tacctttgtc ctctttgtca
    11341 ttcgttttat tatgatctat ggctattatg cctatagaac ccgacgcctc aagaaaaagc
    11401 taaataagta tatgaaggac atgtttctct tgaccttttc aggtgttaag ggaacggtgt
    11461 cgattgctac gattctcttg ataccaagta atctagaaca ggagtatcct ctcttgcttt
    11521 tccttgttgc aggtgtgacg cttgttagct ttttaacagg tctcttggtc ttgcctcatc
    11581 tttctgatga agaggaagaa agcaaggatt atctcatgca tatcgccatt ttgaatgaag
    11641 taacgctaga gttggaaaaa gagttggaag acaccagaaa taaactttcc ctctatgcgg
    11701 ctattgacaa ttatcatgga cgtattgaaa atctcatttt aagccaagaa aaccaggatg
    11761 atcaagaaga ctgggctgct ttgaaactct tgattcttag tattgaaagt gatggtttgg
    11821 aacaggccta tgaagagggg aatattagca atcgtgctta ccgagtttac caacgttatc
    11881 tgaaaaatat agaacaagga atcaatcgta aacttgcctc aagactgacc tattattttc
    11941 ttgtttcctt gaggatttta cgttttctcc ttcatgaagt ttttactctt ggaaagacct
    12001 tccgtagctg gaaggacaag gagcaaagcc gtctccgtgc tcttgattat gaccaaattg
    12061 cagagctcta tcttgccaat acagagatga ttattgaaag tttggaaaac ctgaagggag
    12121 tctacagacg ctctttgatt agttttatgc aggagtctcg tcttcgagaa acagctatta
    12181 tcagcagtgg tgcctttgtc gaacgggtta tcaatcgtgt caaacccaac aatatcgatg
    12241 aaatgctgag aggctattat ctggagcgca agttgatttt cgaatacgaa gaaaaacgat
    12301 taattacaac taagtatgcc aagaaattac gacaaaatgt aaataactta gagaactatt
    12361 ccttgaagga agctgccaat accctgccgt atgatatggt ggaattggta agaagaaatt
    12421 agttaatact cttcgaaaat ctcttcaaac cacgtcagcg tcgccttgga ttatatatgt
    12481 gactgacttc gtcagtttca tctacaacct caaagcaggg ctttgagcaa cctgcggcta
    12541 gcttcctagt ttgctctttg attttcattg agtataagat tataaatgaa ggagtatgac
    12601 atgaaaaagt ggtggaaaga gctgatagac aagcctttat taaaagcttt tttgcattat
    12661 tatcaagcat cagatagtga gttgaccagt gtcgcagtag cctactattg gttgatttcg
    12721 attttccccc tgcttttggt ggtggtcaat atcctcccct attttcagat tcctgtggga
    12781 gaatttctgt gctttatgaa agacgttctg cccccaagcc tctatgaagg tgtggaaaaa
    12841 atagccagag aagttttgac gcaaccttca acaggtttat tgagtttttc tgttttatcg
    12901 gcgttatgga gtttttcaaa atctatgaat ttcttgcaaa aagcttttaa caaggcttat
    12961 ggcgtcgaaa agagtcgtgg acttatttcc catcagatgc taagtctcct agtcagtttt
    13021 ggcttgcagc tcctctttgc gtttgccttg tttttgatct tatttggtca gatgattttg
    13081 gctttacttg ctcattattg gacaaaagat ggcattatct atcaggcttt acaagggttg
    13141 gcaggtcctc tgatttacgc cctcctcttt gctatcttgg tcatgcttta ttattttctt
    13201 cccaatatat ctaacaggaa aattagctat accctaccag gcagtgcctt tgttctctta
    13261 gtgattttag gtttactaac tctgttctct agttatctca attactatgt ccatcatttg
    13321 gtggatgtcc gaattttagg ttccgttctt cttgttgtta tgatgttttg gtttatcttg
    13381 attgctaaaa ttgttatcct aggcgcggtt atcaatgcca gtatgcagag tttgagagat
    13441 ccggtcttta aaaaagacta actatttatc cattacaaaa gcgcatagta tcaaaacttt
    13501 atagaacctt gatactatgc gcttttgtgc tttgacagtt ttttgtatag tttttgtcca
    13561 agatttctaa atcatctgat taaattccta ttttccccta tcattgtccc tgttttttcg
    13621 aaaatggaag gagtataata aatctatcaa taaaatgata ggaggaactt tatgggtcat
    13681 attttctttt ttctaagtgt ctttttggca gggattctat ccttcttttc tccttgtatc
    13741 ttacctttgt taccggtcta tacaggagtg ttactagatg ataaggatgg tgctcaggct
    13801 tctagcggca aattttcaat ctcagttact agtttattac gaactctggc ctttatagca
    13861 ggaatttcct ttatatttat tttgttgggc tatggagctg gttttttagg cgatttgctt
    13921 tatgcttctt ggttccaata tcttactggg gcaattatta tccttcttgg tttgcaccaa
    13981 atggagattc tacactttaa ggggctttat aaggaaaaga ggctacaact gcaaggacag
    14041 gggcaaaatg gtaagggcta tagtcaggca tttttattgg gcttgacctt tagttttgct
    14101 tggacgcctt gcgtggggcc ggttctgggg tctgttttgg ccttggcggc ttcaggtggt
    14161 tcaggagctt ggcagggagc tggtctcatg ttggtgtata cgctgggctt ggcgctacca
    14221 ttcttgcttc tagctctgac ctctagttat gttttgaaac atttccgaaa acttcatccc
    14281 tatctcggaa tcctcaaaaa agtgggtggt tttctcatta ttgtgatggg actcttggtt
    14341 ctgtttggaa atgcttcaat tttaagtcaa ttatttgaat aaaatggaaa ggaatatcaa
    14401 tatgaaaaaa tggcaaacat gtgttcttgg agcaggttcg ctcctttgtt tgacggcttg
    14461 ttcaggcaag tccgtgacta gtgaacacca aacgaaagat gaaatgaaga cggagcagac
    14521 agctagtaaa acaagcgcag ctaaagggaa agaggtggct gattttgaat tgatgggagt
    14581 agatggcaag acctaccgtt tatctgatta caagggcaag aaagtctatc tcaaattctg
    14641 ggcttcttgg tgttccatct gtctggctag tcttccagat acggatgaga ttgctaaaga
    14701 agctggtgat gactatgtgg tcttgacagt agtgtcacca ggacataagg gagagcaatc
    14761 tgaagcggac tttaagaatt ggtataaggg attggattat aaaaatctcc cagtcctagt
    14821 tgacccatca ggcaaacttt tggaaactta tggtgtccgt tcttacccaa cccaagcctt
    14881 tatagacaaa gaaggcaagc tggtcaaaac acatccagga ttcatggaaa aagatgcaat
    14941 tttgcaaact ttgaaggaat tagcctagga ggtgtcttat gaatgataag ttaaaaatct
    15001 tcttgttgct aggagtattt tttctagcca taaccggttt ctatgttcta ttgatacgaa
    15061 atgcagggca gacagatgcc tcgcaaattg aaaaggcggc agttagccaa ggaggaaaag
    15121 cagtgaaaaa aacagaaatt agtgaagacg cagacttgca cgaaatttat ctagctggag
    15181 gttgtttctg gggagtggag gaatatttct cacgcgttcc cggggtgacg gatgccgttt
    15241 caggctatgc aaatggtaga ggagaaacaa ccaagtacga attaattaac caaacaggtc
    15301 atgcagaaac cgtccatgtc acctatgatg ccaagcaaat ttctctcaag gaaatcctgc
    15361 ttcactattt ccgcattatc aatccaacca gcaaaaataa acaaggaaat gatgtgggga
    15421 cccagtaccg tactggtgtt tattacacag atgacaagga tttggaagtg attaaccaag
    15481 tctttgatga ggtggctaag aaatacgatc aacctctagc agttgaaaag gaaaacttga
    15541 agaattttgt ggtggctgag gattaccatc aagactatct caagaaaaat ccaaatggct
    15601 actgccatat caatgttaat caggcggcct atcctgtcat tgatgccagc aaatatccaa
    15661 aaccaagtga tgaggaattg aaaaagaccc tgtcacctga ggagtatgca gttacccagg
    15721 aaaatcaaac agaacgagct ttctcaaacc gttactggga taaatttgaa tccggtatct
    15781 atgtggatat agcaactggg gaacctctct tttcatcaaa agacaaattt gagtctggtt
    15841 gtggctggcc tagttttacc caacccatca gtccagatgt tgtcacctac aaggaagata
    15901 agtcctacaa tatgacgcgt atggaagtgc ggagccgagt aggagattct caccttgggc
    15961 atgtctttac ggatggtcca caggacaagg gcggcttacg ttactgtatc aatagcctct
    16021 ctatccgctt tattcccaaa gaccaaatgg aagaaaaagg ctacgcttat ttactagatt
    16081 atgttgatta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtatg
    16141 tagggttact gacttcgtca gttttatcta caacctcaaa gct
//
LOCUS       DBEJAQ010000043        14894 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.043, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000043 DBEJAQ010000000
VERSION     DBEJAQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.043"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..165
                     /locus_tag="ACL297_001584"
     CDS             <1..165
                     /locus_tag="ACL297_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGR0071827.1"
                     /translation="QLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKK
                     ERTKFVLSRA"
     gene            complement(264..1886)
                     /gene="groL"
                     /locus_tag="ACL297_001585"
     CDS             complement(264..1886)
                     /gene="groL"
                     /locus_tag="ACL297_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGR0071828.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1902..2186)
                     /gene="groES"
                     /locus_tag="ACL297_001586"
     CDS             complement(1902..2186)
                     /gene="groES"
                     /locus_tag="ACL297_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGR0071829.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2342..2737)
                     /locus_tag="ACL297_001587"
     CDS             complement(2342..2737)
                     /locus_tag="ACL297_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGR0071830.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2815..3576)
                     /locus_tag="ACL297_001588"
     CDS             complement(2815..3576)
                     /locus_tag="ACL297_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGR0071831.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3618..4244)
                     /gene="ytpR"
                     /locus_tag="ACL297_001589"
     CDS             complement(3618..4244)
                     /gene="ytpR"
                     /locus_tag="ACL297_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HGR0071832.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(4260..4577)
                     /locus_tag="ACL297_001590"
     CDS             complement(4260..4577)
                     /locus_tag="ACL297_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HGR0071833.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(4574..4873)
                     /locus_tag="ACL297_001591"
     CDS             complement(4574..4873)
                     /locus_tag="ACL297_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HGR0071834.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5071..5151)
                     /locus_tag="ACL297_001592"
     CDS             complement(5071..5151)
                     /locus_tag="ACL297_001592"
                     /inference="COORDINATES: protein motif:HMM:NF039231.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071835.1"
                     /translation="MASSGKMLRNTYTPDGYYVGNLGAWQ"
     gene            complement(5158..5349)
                     /locus_tag="ACL297_001593"
     CDS             complement(5158..5349)
                     /locus_tag="ACL297_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HGR0071836.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5510..6184)
                     /locus_tag="ACL297_001594"
     CDS             complement(5510..6184)
                     /locus_tag="ACL297_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071837.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6190..6637)
                     /locus_tag="ACL297_001595"
                     /pseudo
     CDS             complement(6190..6637)
                     /locus_tag="ACL297_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(6751..7254)
                     /locus_tag="ACL297_001596"
     CDS             complement(6751..7254)
                     /locus_tag="ACL297_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGR0071838.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7251..7613)
                     /locus_tag="ACL297_001597"
     CDS             complement(7251..7613)
                     /locus_tag="ACL297_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HGR0071839.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7679..7798)
                     /locus_tag="ACL297_001598"
     CDS             complement(7679..7798)
                     /locus_tag="ACL297_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGR0071840.1"
                     /translation="MVMKDGNIIEQGNYEELMAQGGFYADLYNSQFTEDEAEE"
     gene            complement(7856..9580)
                     /locus_tag="ACL297_001599"
                     /pseudo
     CDS             complement(7856..9580)
                     /locus_tag="ACL297_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9555..10026)
                     /locus_tag="ACL297_001600"
                     /pseudo
     CDS             complement(9555..10026)
                     /locus_tag="ACL297_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            complement(10150..10866)
                     /locus_tag="ACL297_001601"
     CDS             complement(10150..10866)
                     /locus_tag="ACL297_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGR0071841.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11747..13162)
                     /gene="ply"
                     /locus_tag="ACL297_001602"
     CDS             complement(11747..13162)
                     /gene="ply"
                     /locus_tag="ACL297_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HGR0071842.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13173..13583)
                     /locus_tag="ACL297_001603"
     CDS             complement(13173..13583)
                     /locus_tag="ACL297_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071843.1"
                     /translation="MIKRGDVVALYLPFPTISSDLAVKNHMYICIDNSMTKNKELVKN
                     QTFKPALLTRRLVKNFMIEEPDLARNPFTRPTLIDLDKVFMLDNTGIPTSYLARRRRN
                     VSEELYEEILDYLVQPRLISLNKSEFMQLNPGTY"
     gene            complement(13576..14184)
                     /locus_tag="ACL297_001604"
     CDS             complement(13576..14184)
                     /locus_tag="ACL297_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071844.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14197..14655)
                     /locus_tag="ACL297_001605"
     CDS             complement(14197..14655)
                     /locus_tag="ACL297_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HGR0071845.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            14780..>14894
                     /locus_tag="ACL297_001606"
     CDS             14780..>14894
                     /locus_tag="ACL297_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGR0071846.1"
                     /translation="MNYEASKQLTDARFKRLVSVQRTTFKEMLAVLKTAYQK"
BASE COUNT         4344 a         3446 c         2687 g         4417 t
ORIGIN      
        1 caactacact attctaatgc caaactggaa gcgaccaata atctcatcaa acttatcaag
       61 cgcaatgcct ttggttttcg gaactttgaa aacttcaaaa aacggatttt tatcgccctg
      121 aatatcaaaa aagaaaggac gaaatttgtc ctttctcgag cttagctgac ttcaacccac
      181 tacagttgac aaagagccta taaaaagaag ggttagtcat tccctccttt tgtgtttttt
      241 ataagttgtt ttctatagaa agcttacatc atcccgccca tcatacttgg atccattgct
      301 ggagctgggg ctactggttc tggtttattg gctacgactg cttctgttgt caaaatcaag
      361 ctggctacag atgctgcatt ttgtagggct gaacgactca ctttaactgg atcaatgata
      421 ccttgatcaa tcatgttaac ccactcgcca gttgctgcgt taaatcctat accaagctca
      481 gcatttttca aacgatcgat aacgatagat ccttcaaatc ctgcattgtg agcaatttga
      541 cgaacgggtt cttccaaagc acggagaaca atattacgtc ctgttgcttc atctcctgtc
      601 aattccaagg tagcaacagc tggaatcaca ttggcaagag ctgttccacc gcctgcaaca
      661 ataccttctt caacagctgc acgagtagcg ttgagggcat cttcaatgcg gagtttcatt
      721 tctttcaact cagtttcagt tgcggctccg accttaataa ccgctacacc acctgacaat
      781 ttggccaagc gttcttgcaa tttttcacgg tcaaattcag aagttgtagt ttcgatttga
      841 gacttgataa ccgcaacacg gtgagaaatc gcttcaggat ttcctgcacc ttctacaata
      901 accgtgctat ctttgtccac ggtcactctc gctgcttgac caagagcttc aattgtcgca
      961 tctttcaact caagaccaag gtcttctgtg ataactgttc cgcctgttaa gatggcgata
     1021 tcttcaagca tggctttgcg acggtcacca aaaccaggtg ccttgactgc tactacgttg
     1081 aaggttccac gaatcttgtt caaaacaaga gttggaagag cctcgccatc cacatcatcc
     1141 gcaataatca agagtggacg attgctttgg agaatgcttt ccaaaagtgg caagatctct
     1201 tggatattgg aaattttctt gtctgtaatc aaaatgtacg gattttcaag gtcagccacc
     1261 attttttcgc tatctgtcac catgtactgt gaaaggtaac cacggtcaaa ctgcattcct
     1321 tctacgactt caagctctgt ttccatacca cgtgactctt cgatggtgat gacaccgtct
     1381 ttgccaactt tttccattgc ttcagagatg tactcaccaa ctttttcaga acgagaagat
     1441 acggctgcaa cttgagcgat agcttcttta ttggcaacag ggatggcgtt gtttttcaaa
     1501 gcttcaactg ctgcggcaac tgctgtttca atcccacgac gaataccgat tggatttgca
     1561 cctgctgtga cgtttttgat tccttcacgg acgatagctt gggtcaagac tgttgcagtc
     1621 gtagtcccgt cacctgcgat atcattggtt ttagaagcta cttctgatac caatttggca
     1681 cccatatttt caaaatggtc ttctaattca atttctttgg caatagtcac accgtcattg
     1741 gtaatcaatg gtgagccaaa tgatttttca agaacgacat tgcgaccttt tggtcccaag
     1801 gttactttaa cagtatctgc aaggatatcg acaccacgga ccatagctga acgggcatct
     1861 gatgaaaatt taatttcttt tgacatactt actttctcct tctattcttc aatgattgcc
     1921 aagatgttag cttcgcctac gatgatatac ttttcatcgc catctttgac atcaagacct
     1981 gcgtgggctt caactaagac acggtctcca gttttaacac ttggagcaac caagtcaccg
     2041 ttcaaggtac gaacaccttg tccagtagcc acaacttggg ctgttttggt tttttcttgg
     2101 gctgaacctg cgagaacaaa gcctccaaca gtttgttctt tttcttctac ttttaagacc
     2161 aaacggtccc ctaatggttt caacatctga tttcctccat aatgagatag atattattag
     2221 cactctttat acctgagtgc taattcatag ttctattgta tcacttagtc agaaataatc
     2281 aagaaaaaag tctgactttc tcaagataaa aagcctgaga ccaactcaga ctttttaatt
     2341 cttaaaatgg caattcttcc tcttccaaga ccaaatctgc caaatcttgg cctgcattat
     2401 tttcacgcat agcacgttgg gcacgacttt ccaagagttg gaatcctgtc acaaggactt
     2461 cggttacata attcatctga ccatttttct caaagcgacg ggtacgcaat tctccatcaa
     2521 cggaaatgag actacctttg gttgcgtagc ttgccaaagt ttctgctagt ctgccccata
     2581 ggaccatatt gacaaaatca acttcacgtt caccgttttg gtctttgtaa cgacggttga
     2641 cagcgatagt tgctcgcgct actgacttgt cattgttggt tttgtgcaat tctggtgtag
     2701 acgttaaacg tccaatcaag ataactttat tatacatatt ttcttcctcc tacttatcta
     2761 ttcgtaggaa atcaaaaaaa gttacagaaa tttgtaactt ttcgagaaaa ttttttattt
     2821 tttatgaacc atgaaacctg tcgcctgttg attggccata atggtcatat ctgtaatctg
     2881 aacacgacga ggttgactag tcacatagac tactgtatct gcaatatcct gagcttgcaa
     2941 agcttctatt ccttggtaaa cggacgcagc tcgttcttta tcaccatgaa aacgcactgt
     3001 agaaaaatct gtttcgacaa ttccaggctg aatggttgtc accttgatat ccgttgcgat
     3061 ggtatcaatt cgcagtccat ccgaaaaggt cttaaccgca gccttggtag ctgagtaaac
     3121 agcggcacca gcataggcgt agattcctgc ggttgacccc atattgataa tatgaccttg
     3181 attgactttt atcattgctg gcaagaaaca gcgagtaacc gccatcagac ctttaacatt
     3241 ggtatccaac atggtcaaca tatccaactc ctcatagtct tgatagggag ccaagtcaag
     3301 agccagtccg gcgttgttga ccaagatatc aatcctccct atcgtttcta gaatatcaga
     3361 gcaaacagtc ttcaccatga ccatatccgt cacatctagt ggaaaagtcc agacggtttg
     3421 atttggaaag cttactgcaa actccgactt gaggatttct aatctgtcta tccgtcgtcc
     3481 tgttagaacg acatcctcac cctgctccag ataagcacgc gcaatcgctt caccgattcc
     3541 tgaggttgct cccgtaatca caacattttt tgccatctta tttccttcta gctggtctat
     3601 cagatattaa caacttctta ggcagtccag tgtttagctg agtcaaatgg tgttccgaca
     3661 acttggtctt ctgataattc aagcacccca cgtttttgtg gagcatttgg cagatgcaat
     3721 tcacgaggac tgcacatcat accaaaactc ttttcaccac gaagttcacc tgggaaaatg
     3781 agattccctt ttggcatcat agctccagga agcgcgacaa tggttttcaa ccccacacgc
     3841 gcattgggag ctcctgcaac gatttgtaca gtcttatcac ttgcgactgc aacttggcag
     3901 atgttgaggt ggtcactatc tggatgggct accatctcaa caatttcacc gacaacaaac
     3961 ttaggttcct tatcattaac aatttcttct gtaaaacctt ccgcctgcaa ctcttggttc
     4021 aaacgagcga cttgctcatc tgtcaaaaag acttgaccgc gctctgcagt ttcaaataaa
     4081 cttgaaactt cgaaaatatt ccaagccact gtttccccat tttctttgag gaaaacacgg
     4141 gctaccttgc ctttgcgttc cacattcagt ttggcatctc cgctattttt cacgatgacc
     4201 ataaggacat caccgacatg ttctttgtta tacgtaaaaa tcattgtttc ctttttctcc
     4261 tatttcaatc ctgctaaaaa atcgttaatt tgctccttac ttttacggtc gcgattgaca
     4321 aaacgaccga tttccttgtc cttttctaga acaacaaggc taggaattcc gtaaacatcc
     4381 cagagtttgg ccaaatccat atactgatct cggtccattc gaataaaggt gaactctgga
     4441 ttggtctctt caatctctgg taaggcagga taaatataac gacaatcgcc acaccagtct
     4501 gccacaaaaa ggaagacctt cttgcccgct ttttccacta aagatgctaa ttcttctaaa
     4561 cttgctggct gtatcataag acttcctcct catagactag gtcttcattt tcatagacaa
     4621 aggtataatg acggccatcc tcaaaaatga cgccaccaac caagctctcc agactgcttt
     4681 cgtaaacttg aacataaagg gtcgcaattt cccccatgtc ggaaaaatgg tctcgcacaa
     4741 tctctgtcaa ctcttcctga gtcttcatga gcttacggtc atctgcaact tttttcgtag
     4801 caagggcaag gcttccgata cctagcagag ccaagcctgc catccacatt tttttagctt
     4861 tcataccatt cattttaaca caaaaaaggc ttcaggacaa atgaggaagc agcagaaaag
     4921 caagtaaaaa gcctcttcct ttaaggaaaa ggacttctta tactaagagc aaactagaaa
     4981 gctagccgca ggctgctcaa aacacggttt tgaggttgta gataaggcga cgttgacgtg
     5041 gtttgaagag attttcaaag agtattattc ttattgccag gcacctaagt tgccaacgta
     5101 gtaaccatca ggtgtgtagg tattgcgaag catcttacct gatgaagcca gataatacta
     5161 cttgccattg tctttgaccc aatcattcgc aatcatggaa ccagaagaac ttacataata
     5221 ccattctccc ttgtcataaa cccaagtact gactttcatg gttcctgagc aattaaaggc
     5281 aaaaaaactg tccaatatca ttcgtttttt aaaagcattc gacactacat catctacgct
     5341 gtagtacata gatacatcaa acgtaaaagc caaaatatcg aaaaatatac aacttttaaa
     5401 ggcagcattt cattttttaa agtctaacaa ccatagaact gaaatcagag caaggcatca
     5461 atttcctact ctatagtacg cctattttga tggttgaagt gaagattttt tatttaacgt
     5521 agacaatttc caacttccca aaagccacat ctccacggat aatcaaggtt ttgctggttg
     5581 gggctacagg agcgtcagcc ttagctgcac caaaggaggt ttctactttc aaatctacac
     5641 gccagtgttg tggaacatag acggttgcat ttccgaaggc tacttcaata tttaaagttg
     5701 caaaatcacc tagcatctct gcattatcat agtagatttt agcatcccca aaagcgactt
     5761 ccacttggtc atctacgaga tcttgatctt gcttatagaa ggtcccgcta ccaaaagcga
     5821 cttccttatc cgtgacgact gtttttttcc cattgtacca ccattttttt tcattccaga
     5881 acttacttga atgcgtcaga taaccaacac caagtaccac caagatgcta gcccaaataa
     5941 gagaatgatt ggtaactggt aacaagtcat aagcgtaatt tgcaatgatg agcgccagta
     6001 aacctgtaaa aactgccgaa gtgagatgac gtctaaggat ggactcaatg gacttataag
     6061 caaaaaaaac aatacctagt aaaggccata ttttaccatc caaagaagga attccaaaat
     6121 tcccttgcag caagatccaa gctgctaaaa ccaataaaac aataccaaat gctttctttt
     6181 tcatgttttt tacctcatgt ttcttagatt ttcttttagg agggattggt aatgccgtga
     6241 gacatgaacc tccttgtgcg tctgataaaa ggaaatggtg cccgttcctg aaaaggactt
     6301 gtccactgag taaaatctga cggatgtttg cgatcgttga cttggaaact cgattaaaat
     6361 agcgaggcaa gatagactcc aactcataga gcttgaggcg aacctcataa gcttccttct
     6421 ggttatgagc gtagatcttg ctcccttctg tttcaaagaa gagaatttct gacaggtcta
     6481 gataatattc acctgtcccc ttgtaaaaag tcaacctcgg agcctttgac tcttgcaaga
     6541 gccgttgcaa gtctgcaatt tcatctgtca aagtgggggt cttgatgaca atttcagtct
     6601 cctctaaatt gccgtcaatc tcaattcgta acttcatcac atctcctttc tgactctact
     6661 atatcaaagc taagttagga atacaagggc aaaaaagcaa gtggtcaatt tgaagcagta
     6721 agtggttgta ggactagctt aacttacatg tcagaatagg aagaaaagga atccccacac
     6781 caaaccacaa gcatagccaa ccaagacatc cttgggataa tgaacacccc ctaaaaccct
     6841 caccaacccc agcaaagctg aaaagatcaa gcatactaag ccaataggta gactagcatg
     6901 cagacaggcc atggagatga tagttgccga aaagacatgg cgactgggca tcgaccgtcc
     6961 cgaactatcc ttatcaagaa ggggacagat atcccaagtt tcataaggtc tcgggtgatt
     7021 gattttctta cgaaacaggg acaaggtcac aaaacctgat gcaggaataa gcacataaac
     7081 cccaacttgc tgaccaagtc ctagttgcag gtaagtggtg actagcaaga tcagatagac
     7141 caggggcatg gcgactgtca tcaaacgatt gaaactgcgt aataggaata aaagaattgg
     7201 acgactagtt agtctggagc tgatatttcg ataccattct tgataatttt tcatttatgc
     7261 ttctcatact tttctagaac ttgttgagat gttttcccct tggcaagcca atccactagc
     7321 ttgtccaagc gacgtatttc ctgcatcaga ggatcatcaa tttcctcaat acggacaccg
     7381 caaatcttcc ctgtaattgc aattcgttca gggacataat atggtgcttg tctaaaaaaa
     7441 tctccgtaag taacatccct atccagacaa gcaagaacat cactcacttc ataccctgtc
     7501 aaccaactgg tcacttggtg aactgactct gcctttccac cctttcgctc aacttttgca
     7561 atcaaggcta agtagacagc agcaaatttc atattgtata atttctgact catacttctc
     7621 tctttctatg tgtactagta taaataagaa aaagaaggcc gtcaagcctt cttttgattt
     7681 attcttctgc ttcatcttct gtaaattgac tattgtacaa gtcagcgtag aagccacctt
     7741 gcgccatcag ttcctcatag ttgccttgct cgatgatatt tccatctttc atgaccaaga
     7801 tcaagtctgc atttcggatg gttgacaagc ggtgggcaat gacaaaggat gtgcgtcatt
     7861 ccatcaaacg gtccatggct ttttggatca attcctctgt ccgtgtgtca acagaagaag
     7921 tcgcctcatc caaaatcaaa agcggtgcat ccttaagaag ggcacgagca atagtcaata
     7981 gttgtttttg tcctacagac aaggtcacgg tgtcatccaa gatggtatca tagccatctg
     8041 gcaaggtcat aataaagtgg tgaattccca cagccttact agcttccatc attcgttcat
     8101 cactaatccc tatttgatta tagatgagat tgtctcgaat agttccttca aagagccagg
     8161 tatcctgcaa gaccattgaa aaggcatcat gcacttctga acgcgtcata gccttggtat
     8221 ccacaccatc aatgtgaata cttcccttat caatctcata gaatttcatc aaaagattga
     8281 caatggttgt cttaccagtc ccagtcggcc caacaatggc aaccttttga ccagcatgag
     8341 ctgtcgcaga gaagtcatag tcttgaacat tgacaccgtc caccagaatt tctcctgctg
     8401 acacgtcgta gaaacgtgga atcagattga ccagagttga tttaccagaa cctgttgacc
     8461 caataaaggc cactgtttga ccagtttctg ctttaaagct aacatgttca ataactgcct
     8521 ccgaatttgc cgcatagcgg aaggtcacat ccttaaactc gacctgacct ttgaagtttt
     8581 catcagtcag ctgcacttga acagggtttt ggatagaaga atgcaaatct aaaacttgat
     8641 taatccgctt agcagagacc atagttcggg gaagaacgat gaagagtgct cccatgagaa
     8701 ggaagcccat gacaacctac atggcataag acatgaaaac aatcatgtca ctaaagagag
     8761 gcagacgcgc tatcgaagca gcgtcgttaa tcacataggc cccaatccag taaatcgcta
     8821 cactcaaacc acttgaaatc cccatcatga taggattcaa aatagccata agacggttga
     8881 caaacaaatt caaacgggtc aattcatcat ttactgctgc aaatttttca ttttgataat
     8941 cctctgcatt gtaggcacga acgacacgaa tacctgttaa actctcacga gtgatactgt
     9001 tcagtttatc tgtcaacccc tgaatcaagg actgttttgg aaaggctagc gtcatcaaaa
     9061 cggtcgtcat caggacgttg acaatcacta ccacaagtac ggcccagagt cagtattctg
     9121 aatgacctaa aatcttccca atagcccaga tagccataat tggaccacgc gttaccactt
     9181 gcaagcccat agtaatcaac atttgaactt gagtaatgtc attggtagta cgcgtcaaga
     9241 ggctaggaat tgaaaatttc ttaatctctg tctgcgagta atccaaaact cggttaaaaa
     9301 tatcacttct cagcctacta gtataagaag ccgccactcg ggatgcaaaa aatccaactg
     9361 caactacgga caagaaggca agaaagagca ttcccatcat catgcttgcc ggctgccaca
     9421 actcatctaa attagtttct tgactaccta gcaaatccgt aattttcgag atataggtcg
     9481 gcacttccaa ctctagatag accgaaaagc aagtaaagaa aatggctagt aaaatcatcc
     9541 cccattcttt tctactaatt cttttggtta atttctttat tctctcctcc tattcccttg
     9601 atattttgcc tgtagttgac cgagaacttt ctcaaaaatc agtaattcat cttcatcaat
     9661 gtcttccatc aactgcttgt ctatgcgttc aaaaaaagcc ttaacctgtt gcatctgaga
     9721 acgtgctttg tccgtcagac gaacaaactt agcccgctta tcaacaggac tcgcctccaa
     9781 ttccaccaaa ccattttgca ctatacgctt aaccagatta ctagcaacag acttggtaat
     9841 attgagttcc tgctcgatat ctttaatcaa gaccaagtct tggtttttct agcgattatc
     9901 caaaaaacgc acaacctgac cttgcggccc acccataaat tcaatgccgc aacgtttggc
     9961 ttccttttgc accatcaggt gaatttgatg accaaaacgc ttaaagacta acatcggttt
    10021 atccataatc tcccccttct aaataaaaat agttctctgg agaataatta aatttctatg
    10081 agaactattt tcttgattaa aaaaatccca agtgattttc tcacttagga tcatgttcta
    10141 taggttaaat taaaacccat ctacgttcgt ataaatcttt tggacgtctt cgtcgtcttc
    10201 aagaacgctg taaagttttt caaaggtttc aaggtcttcg cctgacaatt ccacttctga
    10261 ctgaggaatc atttccaatt cagtcacttg gaattcttca ataccagact cacggagagc
    10321 aacgatagcc ttgtgaaggt cagttggcgc tgtgtaaact gtgattgtac cttcttgtgc
    10381 ttctacgtca tccacatcca catccgcttc gagcaattgc tcaaagactg cgtctgcatc
    10441 ttcacctcca aatacaataa cacctttgtt gtcaaagagg taagaaacag aacctgaagc
    10501 gcccatgttt ccgccgtttt taccaaaggc tgcacggaca ttggctgctg tacggttgac
    10561 gttagaagtc aaagtatcca caattagcat agagccattt ggcccaaaac cttcgtaacg
    10621 tccttctgta aaggtttcgt ctgtgtttcc tttggcttta tcaatcgctt tatcgataat
    10681 gtgttttggc acttgggctt gtttagcacg gtcgataacg aatttcaaag ctgagtttga
    10741 ttctggatct ggatcacctt ttttagctgc tacatagatt tctacaccaa attttgcata
    10801 taccttagag ttagctccat ctttagccgt tttcttggct acgatattgg cccatttacg
    10861 tcccattagg aatctccttt tttcacattt taatctttat tattataaca caagtttttt
    10921 tgattttcac tagaggaaat ggattttatt agcaaatcaa gctaggatag cactttacct
    10981 gctaagatgg tcttaccttt ctatctttat caacagacac tcatccacat tcaaaaaaca
    11041 aactagacca ttatctgcaa atagaaagtt tcagccaagt ttgacaaagt cagctcaaat
    11101 tactgtttga agtttgtaga tataagcgac aaaaacaatc atactgcacc ttttgttgac
    11161 agtctactcc agacatatca tagttcaagt aaatactttg aaattcaaca gttcttatag
    11221 gcgctattgt attctaagaa atcaatagaa gagtttctaa gcaaacctct aatactcaat
    11281 aaaaatcaaa gagcaaacca gaaagctagc cgcaggttgc tcaaaacact gttttgaggt
    11341 tgcggatggg gctgacatgg tttgaagaga ttttcgaaga gtataattta cgtgttccca
    11401 agatggagaa gttagactag tacactggca cttctaaacc attgctagca attgatttgt
    11461 tcatatttaa tttcattttt tccataaatg ggtattagat ataaacagca aaatatttcc
    11521 gatacgtgtc gttcttgaat ttccaatcat ctaaaacaag taaaggataa tcaatcccct
    11581 gtatatcaag gaattggcta ccctttttac ttttttacac attctgtttg atagattcat
    11641 tttaacatca cgagcatact ccaatggaaa tcgctaggca agagataaac tttcagatat
    11701 ccgcagagag atcatcgcct ctttttgtcg caagcattct cctctcctag tcattttcta
    11761 ccttatcttc tacctgagga tagagagttg ttccccaaat agaaatcgtc cgcttacgca
    11821 ctagtggcaa atcggttttt tcataaaccg tacgccacca ttcccaggca agcccggtac
    11881 actctctaat tttgacagag agattacgaa cattcccttt taaaggaata ctagtggtaa
    11941 agtgagccgt taaatcctgc ccatttctgt cccaagcctt aggagtcaag acttccttac
    12001 cttgatgatc ataggataat tcattccaag taatataata ttgggcaaca taggcaccac
    12061 tatgatccag cagtaaatct ccgtttctgt aagctgtaac cttagtctca acatagtctg
    12121 tactattttg aaaggtcgca actacattgt cacgtaaaaa agaagttgta taggaaatcg
    12181 gcaagcctgg atgatctgct gtaaagcgac tgccttcttg aatcaagtcc tctaccatat
    12241 ccaccttgcc tgttacaact cgggcacccg aacttgggtc gccccctaaa ataaccgcct
    12301 tcacttctgt attgtccaaa atctgtttcc actctgtctg aggagctacc ttgactcctt
    12361 ttatcaaagc ttcaaaagca gcctctactt catcactctt actcgtggtt tccaacttga
    12421 gatagacttg gcgcccataa gcaacactcg aaatatagac caaaggacgc tctgcagaaa
    12481 ttcctctctg ttttaaatcc tctaccgtta cagtatcttg aaacacatct cctggatttt
    12541 taacagcatc tacgctgact gtataataaa tctgcttaaa attaacaatc tgaatctgct
    12601 tttcgcccga atggacagag ttaaaatcaa tatcaagaga attccctgtc ttttcaaagt
    12661 cagaaccaaa cttgaccttg agttgttcca tgctgtgagc cgtgattttt tcatactgca
    12721 ttctagctgg gacattattg acctgaccat aatcttgatg ccacttagcc aacaaatcgt
    12781 ttaccgctcc gcgaacactt gaattgctgg ggtcttccac ttggagaaag ctatcgctac
    12841 ttgccaaacc aggcaaatca atactataag tcatcggagc acgatcgacc gcaagaagag
    12901 tgggattatt ctctaacaag gtctcatcca ctacgagaag tgctccagga tagaggcgac
    12961 tgtcgttggt agctgttaca gaaatatcac ttgtatttgt cgacaagctc cgcttctttc
    13021 tttcgataac aacaaactca tcgggtagct gattaccctc tttgatgaaa cgattttcaa
    13081 tactttctcc ctgatgggtc aagagtttct ttttatcgta attcatagct agtataaagt
    13141 catttactgc tttatttgcc atcttctacc tcctaataag ttcctggatt gagttgcata
    13201 aactcagact tgttcagcga aatcagccgt ggttggacta agtaatccaa aatttcctcg
    13261 tacaattctt ctgagacatt gcgtcgccgt ctggctagat aagaagtcgg aatgcccgta
    13321 ttatccaaca taaatacctt atctaagtca atcaaggttg gtcttgtaaa aggattacga
    13381 gctagatccg gctcttctat cataaagttc ttgaccaaac gtctggtcaa gagagctggt
    13441 ttgaaggtct gatttttaac caactctttg tttttagtca tgctgttgtc aatacagata
    13501 tacatatgat tcttcacagc caaatcgcta ctaatagtcg gaaaaggcaa ataaagagct
    13561 acaacatctc ctctcttaat caagcaagag cacccccttt tctcctaatg taacatagac
    13621 aggattgacc aagtcttctg attgactcag aatttccaaa gtttgagttt ggcgcgctgt
    13681 caatttagta gcatcttgtc tcttcaatac aaaatgcttg tcgccaataa ccttgacaat
    13741 ataatccttc tccaaagctg actggtaaat ccacatcaga tgttgtctgt cctgagaact
    13801 caagagagaa ggattttcaa gcctcccgat agtctgataa aaatcaaaaa caggagctaa
    13861 ctcctgccaa tctgattggc tagttgtcaa ggctagaaaa agggctttgc gagctgatac
    13921 ttcttggtta gccttgagag ttactttccc ctccaagttt tttagaaatc gggaaactcc
    13981 agaaagcaaa tttttctcta actgcgagaa ataaaaacct ttcgttccca gacataagtc
    14041 tttcatgtcg ctttctctag caaataagag ctcaaacatt tgatagtaaa agaaaaatat
    14101 ctggcactgg gtcgcgctca tcttttcctt atcggcttct ttttttaacc agagcaaggg
    14161 cgacaggtaa ctggattgag acatttcctc tacctcctac tcttttttaa ctggagcatc
    14221 tgcactagct gccacttctt ttgactggat actttcccac tggttaatct cctctgagat
    14281 aagaccttcg catgtcttga caaatagggc aaaagccttg gtctttcctg catatttctc
    14341 cgtttggcat tgatagagga atttttcttt ctccaggagt tgcgcagttt tttggtaaga
    14401 aatccaattt tcctttgcat tatacaaatt gataatcccc tcacacagca agccgagact
    14461 ggataaggca accgaaatca aacggtagcg atcacctggc ataggaatag cacaaaagac
    14521 agctatgagg aaacctgcca cgatttctgt tatttttaat accttatagc gcctacgatg
    14581 ttgaacgctt ttctttaaaa aatgagctat ctgtacgtct aatcgctctg tcaggtacat
    14641 ttcttctggc gtcatattcg taactccttt catttacttt gataaccata gtataacata
    14701 tttgaatatc aaagtatagt aaaaactgga ctataactta ttagacttcc tgcgaaacaa
    14761 aatatggtat agtagttcta tgaattatga agcaagtaaa caactaactg atgcacgatt
    14821 taagcgtctt gttagtgttc agcgtaccac ttttaaagag atgttagctg tattaaaaac
    14881 agcttatcaa aaaa
//
LOCUS       DBEJAQ010000044        13902 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.044, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000044 DBEJAQ010000000
VERSION     DBEJAQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.044"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(1..229)
                     /locus_tag="ACL297_001607"
                     /pseudo
     CDS             complement(1..229)
                     /locus_tag="ACL297_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(291..740)
                     /locus_tag="ACL297_001608"
     CDS             complement(291..740)
                     /locus_tag="ACL297_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGR0071847.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(742..1500)
                     /locus_tag="ACL297_001609"
     CDS             complement(742..1500)
                     /locus_tag="ACL297_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGR0071848.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1615..3594)
                     /gene="stkP"
                     /locus_tag="ACL297_001610"
     CDS             complement(1615..3594)
                     /gene="stkP"
                     /locus_tag="ACL297_001610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase StkP"
                     /protein_id="HGR0071849.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3591..4331)
                     /locus_tag="ACL297_001611"
     CDS             complement(3591..4331)
                     /locus_tag="ACL297_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGR0071850.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(4346..5659)
                     /gene="rsmB"
                     /locus_tag="ACL297_001612"
     CDS             complement(4346..5659)
                     /gene="rsmB"
                     /locus_tag="ACL297_001612"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGR0071851.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5652..6587)
                     /gene="fmt"
                     /locus_tag="ACL297_001613"
     CDS             complement(5652..6587)
                     /gene="fmt"
                     /locus_tag="ACL297_001613"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGR0071852.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6600..8996)
                     /locus_tag="ACL297_001614"
     CDS             complement(6600..8996)
                     /locus_tag="ACL297_001614"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGR0071853.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHARDVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9062..9376)
                     /gene="rpoZ"
                     /locus_tag="ACL297_001615"
     CDS             complement(9062..9376)
                     /gene="rpoZ"
                     /locus_tag="ACL297_001615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGR0071854.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9401..10027)
                     /gene="gmk"
                     /locus_tag="ACL297_001616"
     CDS             complement(9401..10027)
                     /gene="gmk"
                     /locus_tag="ACL297_001616"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGR0071855.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10157..11770)
                     /locus_tag="ACL297_001617"
     CDS             complement(10157..11770)
                     /locus_tag="ACL297_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGR0071856.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11852..12121)
                     /locus_tag="ACL297_001618"
                     /pseudo
     CDS             complement(11852..12121)
                     /locus_tag="ACL297_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004027196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(12253..13011)
                     /locus_tag="ACL297_001619"
     CDS             complement(12253..13011)
                     /locus_tag="ACL297_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071857.1"
                     /translation="MKFKNYLFDLYPYFPKGFSLDNREDPDVCSKALYDDLCKMFFDD
                     DSKEKLKITSVCNKCQNYGNRDYYTLFIDKDKYLLSSDYIGASIYWAQEAGLNDRIIL
                     DHLSISRTIGGHILFPRGGKLETVNQARGGEKGYYDRFDLTLYAIKEWFVENKNTKIG
                     YAIENYHEWFELFSGDDNCKNGFENFVEFFKLEGFIYEQNKIIDLIKSDLENNQVVFL
                     DKEDILIASTEEEYIRYMKNLNIIILERTKKILL"
     gene            complement(13027..13167)
                     /locus_tag="ACL297_001620"
     CDS             complement(13027..13167)
                     /locus_tag="ACL297_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071858.1"
                     /translation="MLFYLTKELNSGVKKIGTISTTLVVVAGVSAGMLGGKFDFKFPK
                     KI"
     gene            complement(13121..13624)
                     /locus_tag="ACL297_001621"
                     /pseudo
     CDS             complement(13121..13624)
                     /locus_tag="ACL297_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370715.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
BASE COUNT         3821 a         3240 c         2587 g         4254 t
ORIGIN      
        1 aatttctttt taccagaatc attcgctaat tgttttttta ggacaattga tttgtacttc
       61 cgtcgcatca atcattaccg tgtcttcaga actgagagga gttcttgaaa tcgtaacacc
      121 acctttgaac aagagttact tcaacccatt ggctccgacg gagtaagttg ctttcgtgga
      181 taccaaaatc agccgcaatt tcttcataag tgcggtattc tcgcacatat agaaagcgtt
      241 gttaatttat ttatctcatt tttcagaaaa ttcttttatt tctgtaaagt ctacgatact
      301 cgatgtgttt ttatataatg atacagtctg agaatcactg ttccgctagc cattccaata
      361 gagattacca aagccaacat gacaaccaag gtcgcacttg ccagtgcttt attatagtcc
      421 cctgtcacaa aaaaggcagt tgttcggtag gagagataac ctggaaccag cggtgccaaa
      481 atggccaaga taaagaccac agcaggtgtc ttataaagaa tacttaaaat ctggctgaca
      541 caagaaccaa taatggctgc aatgaaggta gctacaatga cattggtcgg ttccttgagc
      601 aagagataga ttagccagac agtcatgccc aaaatccctc caggtaagag catagaccgt
      661 tgcacattga gtacgattaa aaaagtgata atggcaagaa aacttgctac tgcttgtaat
      721 aaaaaggttg ttagtgtcat attagttcat caataccaag gcgacagaag ttcctgcccc
      781 taaagcgagg gtaatgagca gggattcaaa catcttactc ataccagagt ttatgtggtt
      841 ggtcataata tcacggaccg cattggtcaa ggcaatacct ggtacaaacg gcatgaccgc
      901 accagctata atcaaatctg ccgttgaagg aaaacctgtg tagcgagccc aaaactgggc
      961 aattatccca aagacaaagg ctccagcaaa ggctgtcaca aagggaattc ggataaattt
     1021 ttccacatag agggaaaagg caaaaccaaa taaggtcgcc actcctgccc caagtgcgtc
     1081 gtagatattt ccgctaaaca taactgaaaa gaaaggagca ctaaaggtcg cagccagagt
     1141 tacctgcaac ttagtatagg gaaggggttt ggcttgcaag gccgtcaatt gcttaaaggc
     1201 tgtttctaag tcaatctgcc ccccaactag ctgacgagaa atctggttca catcgcagac
     1261 tttttcgatg ttataagaag aggaggtcac gcgcttcatg cgcgaaatat tggtattttc
     1321 aatagagaaa aagatagcgg caggcatggc aaggacattg caatccacaa tcccctgcga
     1381 atgcgcgatt cgaatcatgg tatcttctac acgatggatt tctgagccac ttttaaggag
     1441 aatagtcccc gctagcataa tcacatcaat gacggcattt aattcttttg attcttccat
     1501 gctttcctcc ttttatcaac tccctctatt ctatcacaaa tccggactca aaaaaaatct
     1561 ttgccatgaa atcatgacaa agattgatta ctcattttga ttatccatct gcttttaagg
     1621 agtagctgaa gttgttttag gtttgtagat tgaaatcttg actctagtct tatttaggtc
     1681 taccttttca cctgctctag gactttgttc aacaaccatg ccttctacac tacctgcagg
     1741 cgctgtcgtc acttctacaa cttctatatt agcttcctta atcccaacaa tttgaatcaa
     1801 attgttctta gtaaactcca agctggaacc aatgtaactc ggcatggcaa cacttgtaac
     1861 ttttttagct actgtcaaga caatttgagt aggtttactc acatcataag tcgttccggc
     1921 acctggactt tgtttcataa tcgttcctgg ttcgctttcg ctggactctt cttcctctat
     1981 cttaatcaaa ttctcaggaa ccttcttctg cttgagttct gagattactt ctgtagagtt
     2041 ccgtccaata tagttcccta attgaatcgt cgtagctttt ttagctactg tcaaaacaat
     2101 ttgagttgcc ttgctcaagt cataggtcgt accttctggt agactttgct tcaagaccgt
     2161 tccagcctca ctctcattgg actcttcttc ctcaatttta atcaaattat ctggaacttt
     2221 tttctctttt aattccgcga taacatcaga agatttccgg ccgacataat tactaatttg
     2281 gaaggattgt ttgcctgatg agacaaccag attgattttc gttccttctt ttcgaccagt
     2341 tccagcgcca ggatctgtac ggataatccg cccttcttcc accttttcac tagcctctgt
     2401 cttctcctca ccaatctcaa aattggcttt tttgagcgtt gccttggcct ctgcaactgt
     2461 ctgacctgcc acatccggaa tggcaatggt tgcaggagtt ctggataaga tccaaataag
     2521 agaagctgcc accaatacaa ggctggccaa caaaatcagg taacgcatct taaatctatg
     2581 ttttttcggt gcttgtggtt ggtaagtttc ctctgtcaca gcctggcttg ggtttttgat
     2641 tgatttgtgt tctgtctgcg cttgaacctt aggaatagat gtcaaggtac tctgagaaac
     2701 cttcggcaag gtcttggtat ctgccttgct cgtttcatca aagattaact tactttcatt
     2761 tctacgattg taggacaagc tactagacaa gtccacatac atctctgaaa ccgatcggta
     2821 gcgattggtc aactttttag cagttgcctt gataataaca ttttctaaag cctgaggtac
     2881 agatggattt tctgcaataa cggacggcag gggtttctgg aaatgctgga gggcaatggt
     2941 caccgcgcta tccccgtcat aagggatatg gcctgtcaac atctcataga aaataatccc
     3001 catggcatag atatcactct gcacagtcgc cttagaacca cgcgcctgct ctggtgacaa
     3061 gtaatgaact gagcccaaca tcgagttagt ctgggtcaga cttgtctctg caaaggctac
     3121 agcaatccca aagtctgtga ccttggcagt cccatctggt gtcaaaagga tattttgagg
     3181 tttcaagtcc ctgtgaacaa ttcctcgagt atgggccaag cgcatagcca agagaatttg
     3241 tcccatgata cggacggctt cttcattaga aagaggataa tgttccttga tatagcgttt
     3301 gaggtctagt ccagcaacat actccattgc aagatactgt tgaccgtctt cctcaccaat
     3361 atctgttatc cgaacgatat gaggatggtc tagatctgcc atagctctcg cttcacgctg
     3421 aaaacgagct acagctatcg ggtccgtctg gtagttggtc ctcagaacct tcactgccac
     3481 ttcttcccca tctaagatta agtctttggc taggtaaaca tccgccatgc ctcctcgacc
     3541 aatctgtttg acaatccgat agcgtccggc aaaaatcttg ccgatttgga tcattcttca
     3601 tcctcctcgt tcatagaaac aagggcaacc gtaatgttgt ctaaacctcc tgcattgtta
     3661 gcaaaacgaa caagtgtctc cgttttatct gctaaaggaa tatcactggt tacaatatca
     3721 cgaatctcac tgcctgaaat catgttggtc aagccgtcac tattgagcaa gagatagtca
     3781 cctgactcaa ggataactgt cccaaaatca ggctgaattt catctttttg cccaatagac
     3841 tgggtgataa tatttttttg cggatgagct tctgcctctt ctggtgtcaa ttgaccagcc
     3901 ttgagcaatt cattaaccaa ggaatgatcg ctcgtcaact gatggtattc ttctccacga
     3961 atcaagccga tacgcgaatc accaatatga gcatagatag cctgattatc aataatagca
     4021 aggacttcca aagtagttcc catgcctctg taagcttcat cctgaccaag ctggtgaatc
     4081 ttttgatttt caatttctag gtaatgggcg aaccattcac gcacttcatt gactgtatcg
     4141 atctgggtat caacccaagc tacacccagg tctgtgaccg ccatttcact agcgatattc
     4201 cctgcacgat gacctcccat cccatcagct aaaataatca tggtacgtcc agctctattg
     4261 acatagtggt tgacatagtc ttggttattt gttcgtttct gaccaacatc tgttaataat
     4321 gaaatttcca tgtgtcagtt ccttcctaat ccgatatctt gcgaaattga ctgatgaaga
     4381 atccatcact tccatacaat tcaggtgtaa tgaggataca gccgtctttc atgatatcct
     4441 tacattcatg ttctagtttt acctgctcga actcgggatg actctctaaa aaggccttaa
     4501 cgacttgaaa attctcctct gagacgatag tgcaggtgct ataagttatt ataccacctt
     4561 tgcctagtat ttgacaaaca ctacctaata tttctaactg aatttcctgc aaggacgcga
     4621 aatctgccgt ttctttattg tatttgatat ctggttttcg gcgcaaaaga ccgattcctg
     4681 aacaaggagc atccaccaaa atcttatcaa aggaatcctg gtcaaaaaac tcatgcacct
     4741 ttctggcatc caatttttga gtttgaaccc gatcttcaac tcccagacgt tgggcatttt
     4801 cttgaattaa atccaacttg tggtcgtaca agtccagagc agtaacctga cctgtcgtaa
     4861 gataagaggc tatatgggct gttttcccac ctggagccgc acaggcatca agcactcgct
     4921 catcaccttg taaatcaagc gtcggagcaa ccagctgact ggactcgtct tggatggtaa
     4981 tggctccatc cgcaaacaaa ttatgccctg caaaatgccc ctgctcctta accagaccag
     5041 tggttgctaa aagggaatca ttcgcctcca acaaggcttg gatttcctct tttcgactta
     5101 ggtctgttac acgaatactg gctttgtttc gcactaacag gctttcaaag atggcttttg
     5161 ctctctcctc tccgtattct tccttgagtt tggcaactag ccaaactggg agagaatagg
     5221 caatggagtc acgcttgttt tttcgcttga tgctagcaat atctggccag ccttcacgca
     5281 agatacggcg aaggagggca ttgaccaatt tttcactgcc ttttttacga agtttggcca
     5341 attccactgc ttcattgacc acagcatgat ctggaatctt gtccaaatag cggagttggt
     5401 aggcactcat gagaagaagg acatagagcc agctgtctaa ctggtctctg tcttcgataa
     5461 agtgggatag gtaccattcc agagtcagtt tacgggctac cgttccatag accagctcgg
     5521 tcactaagcc cttgtctgct gccaaaagtt gactcccctt tagatgctta tttaaggcga
     5581 tatttgagta tgcttggttc acaaaaacat cctctagcac tgctagagct aaacttctag
     5641 ccgtttctac tttagtcacc aaatcgttct cctacagtca atgtacgtcc aactccgttg
     5701 aggaaggaag caatgtccat cttaggctta ccagctggct gcacttgttt gagggataga
     5761 gccccttcag ccgttgcgac aatcaattct ttcttgccga tagagagaat ctcacctgga
     5821 tttccctgac cttctactgg tagggcttca taaatcttaa agcggtcgcc cttaaggaaa
     5881 gcatgggcaa caggccaggg gttcattcca cgaatttggt taaagagttg acgattggtt
     5941 ttgttccagt ccagtttttc ttcctctggc tttatatttg gagagaaggt aacctgactc
     6001 gtatcctgcg gttcaggttt gatatcacca gcaatatagg caggcagagt gtccaaaagc
     6061 aaatcacgac caactagcgc caatttttca aacaaggtgc caacattgtc ctcatctgtg
     6121 atcggaatgc tgcgacgaga aatcatatct cctgcatcca tttccttaac catttccatg
     6181 atggtcacac cagcttcctc atccccttga atcaaggcat aatggatagg cgcaccacca
     6241 cggtgtctag gaaggaggga ggcatgaacg ttgacagcaa agtccatgct atcaaggagt
     6301 ttgcttggga gaaactgccc aaaagcagca gtcacaattc catctgctcc tagcttcata
     6361 agttcttcca tctctggact tccagataat ttttcaggtt ggtagataga tagtcctgct
     6421 tccttggcag cctgcttgac tggggtttct tggataactt ttttacgacc aacagcacgg
     6481 tctggctggg tcacaacggc tagaatttcg taacggtcat ctgtcaaaag tccttttaag
     6541 actgttgctg aaaagtcggg ggtccccata aagattagtt ttgtcatatc ttctccttct
     6601 tataaaaatt gctgcggctc atggtcaatg ctgagacgga gctcactatt ttcccgttct
     6661 tgagtcaagg ccaagacctg gttgagggtc ggccccagct catcttctaa acggtattta
     6721 attaaaatct ggtaatgata gaggttgtgg gtacgggcaa tcggttttgg cgttggcccc
     6781 agaatgggac tggtctctga caagcctgac cgcaaaatgt tcatgacttc ataggcacgt
     6841 ttgaaaacct cttcttcttt cttgtgagaa agggtaatac caatcgtgaa atagtaaggt
     6901 ggatagccga gttgtcgtct gattcccatt tcataggcat aaaagccttc gtaatcttga
     6961 tccttggcaa atcgaatagc atagtgctgc gaattgtagg actgtatcaa gacttgacct
     7021 gctttttcag cccgacctgc tcgacctgcc acctgagtca agagctggaa ggttctctca
     7081 gaagaacgga aatcaggcag attcaaggcc gtatccgcat ttagaactcc gactagggta
     7141 acattgggaa aatccaaacc ctttgcaatc atctgagtac caagtaaaat atccgcttcc
     7201 cctcgcccaa actggtcaag caaggcttgg tgactgcctt tctttcgagt cgtatccaca
     7261 tccatcctca aaatgcgagc ttggggaaag agttctgcta gctcatcata agccttctga
     7321 gttcccgtcc catagtaacg aatactgcgg ctcttacagt taggacagac ctgaggaata
     7381 tccttcgaga aaccacaata atggcagttc atagtcttgg tatccatatg caaggtcaga
     7441 gaaatatcgc agttgggaca agtatccacc gtcccacact cccgacacat gacaaagcta
     7501 gaataaccac ggcgattgag catgagaacc acctgctctt ttttaaccag acggtcttgg
     7561 atagcctcta gcaaaggagg cgtaaagttt gacgtctcat tttgtccgat atagtctcga
     7621 aagtcaatca cttgaacctc agggattgta gccaaaggat tagcacgttg ggttagacgt
     7681 aagtgttgat agacgccttt gccagcacgt gcccggctct ctaagctcgg cgttgcagat
     7741 ccaagtacca gagttgcttg attatactga gcccgtaaaa tagctacatc tctggcatgg
     7801 taacggggat tgctgtcctg cttataagcc acttcatgct cttcatcaat aatcatgaca
     7861 cccagatttt tcagaggagc aaagatagca gatctggcac caacaacaac ttgggcatcg
     7921 ccacgctcca ccttgcgcca ttcatcatac ttttcaccat tggataatcc tgagtgaaga
     7981 atggctacct tgtccccaaa acgtgctata aaacgctcgg tcatctgagg agtcaaggaa
     8041 atctcaggta ccagcaaaat agctgtcttg cccttatcca gggcaccttg gataatctgc
     8101 aagtaaacct cggtcttccc acttcctgta atcccttgaa gtagaaaggg aggttgagaa
     8161 ctgccaatag aactcacaac cgcatcacgc gcctgtcttt gttctggatt taactccaaa
     8221 ggtctacttg cttcaattcc ttcaaaataa gcagccgagc gttgaacttc cttttggact
     8281 atggtaacag caccttgatc cacaaagaag ttgacttgct ctcgcgagta ggactctaac
     8341 aagctagcca aggaagcgct ctctggatga gacagcagat aatctctcag ttccaacttt
     8401 ttcttggcac gtgtagaaat ctcaacacct tctaattgag catggtcaac ctcataccaa
     8461 gactgggtct tgaccttctt ttgatcgact gcctgatatt ccagaccaag caggcctttt
     8521 ctagtcaaac gcatcatttc agcttgcttg gcaaggtcta gtgaagaaaa ggctagcgaa
     8581 tcttctgaac caaacaggcg cactcgttct tcctgactca agccttccag aggatagaga
     8641 atcttgtcat agctagaatt cagaaatcct ggaagcatgg ccttgaggat agaaattttg
     8701 taggagaaga cagatttgcg taactcctca gccagccaga gttgttctgg cgtgagaaca
     8761 ggagaaaaat ccagcacctc tgcaatatct tttaaatctt gctccatctc ttctccatct
     8821 gattgggact ccaaaccaag aacaatccct tgaatcaggc gattcccctt accaaaaggc
     8881 acatgaaccc gcatcccaac ttccagcatt ccctcaaatt cctccggaat cctgtaacta
     8941 tagggctggt ccgtctgcat caagggcaca tctacgataa tcttagctag ggccatcgtc
     9001 tcacctcctc cttgtcagta cattcttgca atagaaaaaa taagattgag tccccccaac
     9061 cttaaatttt ttcaccatct tctttttctt tagcaatttg ctctttgatt ttcttttctt
     9121 cttcttcttt gcggcgtttt tcttcttcga tacggcgacg cactgcttca cgttttcctt
     9181 ctggatctgg gtgaattgta acgtttcctg attcgatttc ttctaaagcg cgaagagttg
     9241 atttttcaga cttaaaacct tgagttgctg gggcacctgc ttccaattcg tgggcacgtt
     9301 ttgcttccaa gattacgagt gaatattttg aaggaacctt gtcgagcaag gtatcaatag
     9361 agggttttaa catcatttgc ttgtacctat tttctaaatt ttatcgggta gttggagatt
     9421 ttggtaacat ctcctgatag tgaccaatga cacgatccac acagaagtgt tctgcttcaa
     9481 tcacacattt gacacgttca gcagctaggg gtacctgatc gttgacaatc gcataatcat
     9541 actcacgcat gagggcaatt tcttccttgg ccttttcgat tcgttgggca atcacttctg
     9601 cactatctgt tccacgacct accaagcgat cttgcaattc atccaaatct ggtggtgtca
     9661 ggaagataaa gacagcatct ggaacctttt tcttgacctg aagagcaccc tgaacttcaa
     9721 tttcaaggaa aacatcgatt cccttgtcca aggtttcatt gacataggtc agaggagttc
     9781 catagtagtt gccgacatat tctgcgtatt ccaacatctg tccttgacga atcagctctt
     9841 caaattcttc acgagtacgg aagaaatagt caacaccgtc cacttctcca ggacgttgtg
     9901 cgcgtgtcgt catcgataca gaatattgaa attggttttc agaactctca aaaatctctc
     9961 ttctaaccgt tccttttcca acccctgaag gaccagaaaa aacgattagt aagcctcggt
    10021 ctgccattgt gtctcctttt agtcaatctg tgaaataaca tttctctaga ataatggcaa
    10081 aaagccagat tatcctttac agtctttcta tctagtgtaa caaaaaagca gtaatttttc
    10141 aactgctctt tcttatttat ttagcataat ctactgcacg aagctcgcga atcacggtta
    10201 ccttgatatt tcctggataa tcgagattgt tttcaatttt cttacgaact ttgtgagcca
    10261 agattgtgac tttgtcgtcc ttgatttttc ctggattgac catgatacga atttcacgtc
    10321 ctgcttgaag ggcaaagcta gtttgcactc cttcaaagcc gttagcaatt tcttccaaat
    10381 catggagacg cttgatgtag ctttcaagag actcactacg agcacctgga cgggctgcgc
    10441 tcaaggcatc tgctgcagcg acgataactg ctatcacgct ctcagcttca acatctccgt
    10501 ggtgactagc aatcgtattc accacaactg ggggttcctt gtacttacgg gccaattcca
    10561 taccgatttc aacgtggcta ccttcaacct catggtcaat ggctttcccg atatcgtgaa
    10621 ggaatccagc acgacgggca agagccgcat tttcaccaag ttcgctcgcc atgataccag
    10681 ccaacttagc aacctcaatc gaatggcgca aaacattttg tccatatgaa gtacggaact
    10741 gcaaacgtcc cataatcttc atcaagtctg gatgaaggtt tggcgcacca atttcatagg
    10801 cagcagcctc accgtattca cgaatcttat tgtcaatctc ttgacggttt ttctcaacca
    10861 actcttcgat acgagctgga tgtatacgac catctttgag caacatttcc atagtcatac
    10921 gggcaatctc acgacgaatc ggatcaaatc ctgacaaggt caccacttct ggtgtatcgt
    10981 cgataatcac atcgacccct gtcaaacttt caaaggtacg aatgttacga ccttcacgac
    11041 caataatgcg tcccttcata gtatcgtctg gcagatgaac tgttgagttt gttgactccg
    11101 ctacatattc accagcgata cgttgcatag cttgaaccaa gatgtccttg gccattttgt
    11161 cagaacgttc cttgacctct tgctcagctt cgcgaatgcg actggcaatc tccctggtca
    11221 agttttcctc tgtctgagcc aagataatat ctcgtgcttc tgcctgagac agagcaccaa
    11281 tacgctctag ttctgcttct ttttgtcttt cgacttcctc taattgctct tcacgcgcat
    11341 caaggttttt cgctctatca gaaatacttt gttctttttg ttcaagtgtt tgttctttac
    11401 tcgtcaaatt gtcgtcctta cggtcaaggc tagtagctct ctctgtcaaa cgactttcga
    11461 tttgtttgag ttcttgacgt tctgatttga attcagcgtc cacttcttca cggtattttc
    11521 tggcttcttc tttggcctcc aatagtgctt cttttttaag agacttgctt tcacgtttgg
    11581 cttcattaac aagtaaatcc gcttcacgct cagcttgtcc acgtaaatta gttgcttctt
    11641 gttcagcatt taaaagcatc aactctgcag cttcctgaga tgatttcatc ttagctgaga
    11701 tgctgacata tccaatgact aaaccaatga tgacggcaaa aacagcaatc gcaagcgaca
    11761 tgatttccat gtttttacct cattttattg ttattccgaa tgacatacat tcttttacat
    11821 tctaccataa aaaaagaatt taaacaaacc ttaaatggat atcgctaaca tccgcattat
    11881 cactatcata gttatcattt acagatataa agcgaatacc tttttcagga aatatctttt
    11941 gtaagtactt tcctgactcg atataatctc tgccgaaacg agataaatct tttaccataa
    12001 tgatagaaaa ctttccttta ttcaggtcat caatcatatt cttaaaattt ggtcgctcaa
    12061 aattagaacc actatatcca tcatctacat attcatagga taattcaata gatttctctt
    12121 ctacctccaa aaagcaaaaa ggataaattt cccctacact ttaatagtga aaatttatcc
    12181 ctcttggtaa gctcttttat taattttttt gataaagaaa acgcacattt tatttaactg
    12241 gtgctgaaat ctttataata aaattttttt ggttctttct agaataatga tattaagatt
    12301 tttcatatat cttatatatt cttcttctgt gctagcaatc aaaatatctt ctttatctaa
    12361 aaagacaacc tgattgttct ctaaatctga tttaattaaa tctataattt tattttgctc
    12421 atatataaat ccttctaact tgaaaaactc gacaaagttc tcaaatccat ttttacaatt
    12481 atcatcacca gaaaatagtt caaaccattc atggtaattc tctatagcat aacctatttt
    12541 tgtattttta ttttccacaa accattcttt tatagcatat agtgttaaat caaaacgatc
    12601 ataatatcct ttttctccac ctctagcctg attaacggtt tctaattttc cacctctagg
    12661 gaaaagaatg tgtccaccta ttgttcttga aatactcaaa tgatctaaaa ttattctgtc
    12721 gtttaatcct gcctcttgtg cccaataaat agaggctcct atataatcac ttgataataa
    12781 atacttatcc ttatcaataa ataaagtata ataatctcta tttccatagt tttggcattt
    12841 attacataca gatgttattt taagtttttc ttttgaatca tcatcaaaaa acatcttaca
    12901 taaatcatcg tacaacgcct tactacatac atcaggatcc tctctattat ctaatgaaaa
    12961 acctttggga aaataaggat ataaatcaaa tagataattt ttaaacttca tatttattat
    13021 tctccattaa atctttttag gaaatttaaa gtcaaatttt cctccaagca tacccgcact
    13081 tactcccgca actactacaa gtgttgtact aattgttcct attttcttca cgccactgtt
    13141 tagctctttt gttaaataaa acaacattga tcatatctgc ttcacttaca tacttgtaag
    13201 acaactgttc gttggtaaga tcttttaaga gatattcttt gattgcatct gtatgaattt
    13261 tgtagttaat cttagaaatt tctctattca aattccagcc taatgatagc ttagaatttt
    13321 aatctgactt aagtcttttg taatcctgaa ttacatacat cttaaattca gcagaaatac
    13381 ataaagcaaa ttcaaaggca atatctgtat gtgcaaatgc accaccatat cttccacctt
    13441 ttgagataat tccgattgct ccagttttct ctacccattt cttaggagac aatgtaaatg
    13501 aattatatcc agcttccgat ttaattcggt cgaattcgac cgaattgaaa ttcatgttat
    13561 ttaagctttt ccataaacca agaaactcaa ttgtatcttt tcttctcatc caatttttaa
    13621 ttacatcact cgatacttat ccgaacgata agctccacgt aaggtgttcg caatcgacca
    13681 aactgcattg actacatttg aaatctgaac atcgttcgac tggaatatcg gtagtttttc
    13741 tttaaaatta gacatttgct tcccctgttt ttatgattta caattctctt cattatacca
    13801 caaaataggc gctttgtcaa ctgtagtggg ttgaagtcag ctaagctcga gaaaggacaa
    13861 atttcgtcct ttcttttttg atgttcaaag cgataaaaat cc
//
LOCUS       DBEJAQ010000045        13578 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.045, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000045 DBEJAQ010000000
VERSION     DBEJAQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.045"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            72..1331
                     /locus_tag="ACL297_001622"
     CDS             72..1331
                     /locus_tag="ACL297_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HGR0071859.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1407..1542
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1581..2081
                     /gene="rplJ"
                     /locus_tag="ACL297_001623"
     CDS             1581..2081
                     /gene="rplJ"
                     /locus_tag="ACL297_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGR0071860.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2157..2525
                     /gene="rplL"
                     /locus_tag="ACL297_001624"
     CDS             2157..2525
                     /gene="rplL"
                     /locus_tag="ACL297_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGR0071861.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            2712..2882
                     /locus_tag="ACL297_001625"
     CDS             2712..2882
                     /locus_tag="ACL297_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071862.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2882..3130
                     /locus_tag="ACL297_001626"
                     /pseudo
     CDS             2882..3130
                     /locus_tag="ACL297_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3144..3484
                     /locus_tag="ACL297_001627"
                     /pseudo
     CDS             3144..3484
                     /locus_tag="ACL297_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3477..3857
                     /locus_tag="ACL297_001628"
     CDS             3477..3857
                     /locus_tag="ACL297_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGR0071863.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            3870..4103
                     /locus_tag="ACL297_001629"
                     /pseudo
     CDS             3870..4103
                     /locus_tag="ACL297_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            4106..4693
                     /locus_tag="ACL297_001630"
     CDS             4106..4693
                     /locus_tag="ACL297_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGR0071864.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            4895..5032
                     /locus_tag="ACL297_001631"
     CDS             4895..5032
                     /locus_tag="ACL297_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HGR0071865.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            5211..7820
                     /locus_tag="ACL297_001632"
     CDS             5211..7820
                     /locus_tag="ACL297_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGR0071866.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKSMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            7853..9793
                     /locus_tag="ACL297_001633"
     CDS             7853..9793
                     /locus_tag="ACL297_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HGR0071867.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGISSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRAPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAADHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            9790..11397
                     /locus_tag="ACL297_001634"
     CDS             9790..11397
                     /locus_tag="ACL297_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0071868.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            11412..12152
                     /locus_tag="ACL297_001635"
     CDS             11412..12152
                     /locus_tag="ACL297_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071869.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            12155..13474
                     /locus_tag="ACL297_001636"
     CDS             12155..13474
                     /locus_tag="ACL297_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071870.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDILIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
BASE COUNT         4550 a         1895 c         2666 g         4467 t
ORIGIN      
        1 acacctacgg caaagtgaat ctgaagtggt ttgaagagag tacaacttgt cttttaaaaa
       61 aggagtctat aatgaaagtc tttcagcatg taaatatcgt gacttgtgat caaaatttcc
      121 atgtttatct tgatggaatc ttagcagtca aggattctca aatcgtctat gtcggtcaag
      181 ataagccagc gtttttagaa caagctgagc agattataga ctatcaggga gcttggatta
      241 tgcctggttt ggtcaattgt cacacccatt ctgcaatgac aggtctgaga gggatccgag
      301 atgacagcaa tctccatgaa tggctcaatg actatatctg gccagcagaa tctgagttta
      361 ctcccgacat gactaccaat gcggtcaaag aagccctgac agagatgctc cagtcaggaa
      421 caacaacctt taacgatatg tataatccca atggtgtgga tatccagcaa atttatcagg
      481 tggtgaaaac ttccaagatg cgttgttatt tttctccgac tctcttttct tcagagacag
      541 aaacaactgc tgagactata agcagaactc gatccatcat agacgaaatc ttaaaatata
      601 aaaatccaaa tttcaaggtt atggtagcac ctcattctcc gtatagctgc agtagagact
      661 tgctggaagc gagtttggaa atggcaaaag agctaaatat tccgctccat gtccatgtgg
      721 cggagaccaa ggaggagtca ggaattatcc tcaaacgata cggcaaacgc ccccttgctt
      781 ttcttgaaga actgggttat ttagatcatc cgtccgtatt tgctcacggg gtcgaattaa
      841 acgagagaga aattgaacgc ttggcatctt ctcaagtggc tatcgcccac aatcctatca
      901 gtaacctcaa actggcatca ggaattgctc caattatcca gctccaaaaa gcgggagtag
      961 cagtcggaat tgcgactgac tcggttgctt ccaataacaa tctagatatg tttgaggaag
     1021 gaaggactgc agctcttctt cagaagatga aaagtgggga tgccagccag tttccaatcg
     1081 aaacagctct caaggtactg acaatcgaag gggctaaggt ccttggaatg gaaaatcaga
     1141 taggaagtct ggaagtcggc aagcaagcag attttctggt cattcaacca caagggaaaa
     1201 ttcatctcca acctcaggaa aatatgctgt ctcacctggt ttatgcagtt aaatctagtg
     1261 atgtagatga tgtttatatc gccggagaac aggttgttaa gcaaggtcaa gtcctgacag
     1321 tagaacttta aaagaaaaat cacgaaaaat tttaaaaaaa gtttgcaaaa atcttgcatt
     1381 ctttttttga ctatgctata cttatatacg gtttaaaaaa actgcctaag acagtagggg
     1441 agatcgactc ataaatatcc taccgaggac aaaacgtatc atgtaaaaag aagcgtattg
     1501 tactttcgtg tctaggtttg ggcgcgtttt tctttttgaa aaattcccca agcaaaataa
     1561 ttacggaggt gaacacacta atgagtgaag caattattgc taaaaaagcg gaactagttg
     1621 acgtagtagc tgaaaaaatg aaagctgctg catctatcgt cgttgtagac gctcgtggtt
     1681 tgacagttga gcaagataca gttcttcgtc gtgagcttcg tggaagcgaa gttgagtata
     1741 aagttattaa aaactcaatc ttgcgtcgtg cagctgaaaa agctggtctt gaagatcttg
     1801 catctgtatt tgttggacca tctgcagtag cattttctaa tgaagatgtt atcgcaccag
     1861 cgaaaatctt gaacgacttt tctaaaaacg ctgaagcact tgaaattaaa ggtggtgcaa
     1921 tcgaaggcgc tgtcgcatct aaagaagaga ttcttgcact tgcaactctt ccaaaccgcg
     1981 aaggacttct ttctatgctc ctttctgtac ttcaagcgcc agtgcgcaac gttgctcttg
     2041 cagtcaaagc ggttgcagaa agcaaagaag acgcggctta atcttaagct acatagcgta
     2101 gcctagctac gaaaaaaact attataaaat ttaaaactta tttggaggaa ataacaatgg
     2161 cattgaacat tgaaaacatt attgctgaaa ttaaagaagc ttcaatcctt gaattgaacg
     2221 accttgtaaa agctatcgaa gaagaatttg gtgtaactgc agctgctcct gtagctgttg
     2281 ctgcagctga tgcagctgat gctggtgctg ctaaagattc attcgacgtt gaattgacat
     2341 ctgcaggcga caaaaaagtt ggcgttatca aagttgtacg tgaaatcact ggtcttggtc
     2401 ttaaagaagc taaagaactt gttgacggtg caccagcact tgttaaagaa ggcgttgcaa
     2461 ctgcagaagc tgaagaaatc aaagctaaat tggaagaagc tggagcttca gttactctta
     2521 aataatagag tatcgcaatt tgattgcgga atccctattt accagtcttt gagagcgata
     2581 agcgattgat agaaactgat aaattgattt ggtttcctgc caaaaattct ttggcaggat
     2641 ttttgtttta ataaactttc gggggtagac actagattgt ctaccccttt ttttgtgttt
     2701 cggaggtaat tatgcaaaag gaaggacagt catcacgtca attcgtgaag agtcatctca
     2761 tgactatcat gggagttgat attgtaaaag caactcagtt agttgatgaa atggaacaag
     2821 taggtttaat ccgatttgat gaatttggaa atgttggaat tttagtcttg gagggacaat
     2881 aatgaagcgt attaccgcta atcaatatca gacatcagag cgttattata agctccccaa
     2941 acttttattt gagagtgaac ggtataagaa tatgaaactg gaggttaagg tggtttactc
     3001 agttttaaaa gatcggttgg agttgtcttt gagcaaaggt tggattgatg aggatggggc
     3061 tatttatttg atttattcca attcaaattt gatggcactt ttaggctgtt caaagtcaaa
     3121 attactctcc atgtgagttt gaagtgacat ttttagatga ttaccataaa aaacataact
     3181 acccactatt ttacgaatcc tatcttcaaa acgttatgga attccttgaa agtcaagaca
     3241 taaagaatgg ggttgatgcc tttgtagatg atcatcaaaa tctcgttttt gttttatatg
     3301 gacaaggcta tcgagccgag ggaaaagagg gaatacttac aacccaagta actgtaaaag
     3361 cttatgatga agacaagaaa ccgattaact tcgcaaattt attagattcc ttaatttatt
     3421 agattcctta atcgtgtcag aatatcaaat ggaaccgaat ctttgggagg tctcccatga
     3481 ttgatctcta tctaagtaaa aatagacaaa gaaatcaact tcttttagat ttcttccaaa
     3541 actatggcat agaggtatct tgtcattcag tttctgaaat gacaaaggac aaattaattg
     3601 agatgatgag ctattcttca gattgttttg agtttttatc tcccaattta ttacgattta
     3661 agaaccgtga caatctaaga ttaacggatt tcgttgagat aattttaaaa aatcctgaac
     3721 taaccatcag acttcctctt gcggtttcaa ataagcgagt ttatccaagt ctgaatttgg
     3781 aagaggctag agctctattg ccaagagata cgaaacaatt gatttacatg gcgcaaacac
     3841 attatttatc taactaaagg agaagcaata tggctgaacg attttgggag aacttgtcca
     3901 ttattttggc tgaaagaaat atcagctgga ttgagttaac cagaaaaatg tttgcgggag
     3961 agtttcacta tccaagtgaa ttgaatcgtt tgtatcaaaa gattcgccac tagaagatgg
     4021 aacaatgaat gcctcaaagt ccatgggtag aaaggattgt gcaggtatta gatttagatt
     4081 atgaagattt atttcggagg taactatgaa aagaataatt ccagtttata tattccaaca
     4141 agtaaatgtc ctattggtat ctctatactt attgaaattt ctttgtatcg gtgagttaac
     4201 tatactacag attctctatg gttcgtcact catttctttt ttatggatgt atggccaacg
     4261 caaacaagcg cataaggtca atatgaaatc taggatgaaa tggcttggtg ttgaatttgt
     4321 tagcctactg attataagtc tatgttttag tctgattcat gctcaaggaa gcacgaatca
     4381 agcaaactta attggccttc aacatcaaat tccttggttt tcatttttat tgttcttaat
     4441 caatgcgagt atggttgaag aatttctgta tcgagaaatt ttatggaatt tggtcagaaa
     4501 attagatatc agagttgctt tgacaagtgt tttatttgcc ttagcacatc atacaggaac
     4561 cattctagct tggtgtttgt atgtttcact tgggatgttt ttaggtatgg tgcgctataa
     4621 atctgactta tggggcagta tgggactaca tttggtgtgg aacctactag tttatagttt
     4681 gctgcttttt taaaataggt gacctgattt ttaggtaact tctaaaacta acttccagtt
     4741 ttgggagaga gatggaagtt actttgaaaa gttacggaaa cattattttc tatgtgacaa
     4801 cattgtataa ttgcttgcat tttactggtg gagagagtat aatgagggtg gataaagtac
     4861 ttaattccaa aaatttaaat aggaggagct agctatggca gaagaagtat taaatcttca
     4921 attagtatct gttcaagttg acgaaacaga tgaagttgat ggaatgcgat ttagtacatt
     4981 cagtacaaat cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta
     5041 aattgtaaac tttagagatt ctctggagtt atggatgtcg ttccaatatg tgcacgttgg
     5101 aatgttagtg cttatatggg gagaatccaa aggattctcc ctttttattt aaataagtct
     5161 gtctagctat agatatatta agtttgataa aatttcttgg agattttgtt atggattata
     5221 atttcaattt ggagcatcct ttctttttca ctaataatga ttatagtaca gatactagca
     5281 taaagtacca agtaagtctg ccttttaatt ggcacgaagt aatgaataat gatgagtggg
     5341 tttatcaata ccctattgga aaatttgttg aaagacaagg gtggaaaata cacattagtt
     5401 cagagtataa cagttcacat gaattgttac aagatgtagc aaaaatttgc catgaaatgc
     5461 gaattccatt taaacatctt agtacagaag acaaatttat catgcgaaac gggaaattgg
     5521 ttagtcgagg attttctggt aaatttatta catgttatcc aaatcaaaat gaattagagt
     5581 cggttttgca aaggttagaa agtgccttaa agcaatataa tgggccatat attttaagcg
     5641 ataaacgttg ggatgaagca ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg
     5701 atgatgaaaa gaaggttgct attgatgaat taattgttgg cgatgaagtt gttaaagatg
     5761 aacgtttacc agtatttaaa attccaaaag gtatagtgcc ccctgacttt ttgaataaat
     5821 ggcttgacaa gaaagataaa aaacagggag actttccctt tattatagat aatgcaatta
     5881 gattttcaaa cagtggtggg atatataatg ctaggctaaa agaagatggg aaaaaaataa
     5941 ttttaaaaga ggctagaccg tatacaggtc taggatttga tggtacttat tcttcagaga
     6001 aattagcttc ggagtgcaaa gcactaaaaa ttctaaatga atggagtgag acgcctaaaa
     6061 tatactggca cggtaagatc tgggagcaca ccttcttagg aattgaacat atgaagggcg
     6121 ttccactaaa tagatgggtt accaataatt ttcctttata tgaagttgtt gataagacca
     6181 aagattatct attgagagtt tcaaagatag tagaaaaact catcgattta acgaataagt
     6241 ttcattctga aaatgtatat catcaagatt tacatttagg aaatatcttg gttaaggatg
     6301 aagatgagat tagtataata gactgggaac aggctgtctt ttcaaatgat gaaaaagttg
     6361 tacataaagt tgcagcccct ggttttagag catggcgaga aacacttccc agtgagatag
     6421 attggtatgg tattagacaa atagctcatt atttatacat gccactagta actacatcgg
     6481 atttgacata caactatgta agtcaaacca ggatagaagg aaaaaaacta tttgagtctt
     6541 tgggttatac aagagaacat atagattatg tagaaagttt attgtcatat ctagattcta
     6601 aatgtccaca gattgagaat atttccagga aaaaagtttt aaaatccatg catgagatta
     6661 gaactatcga gagtgagcaa gatatacaag attttattat aaaattattg agagggttta
     6721 cgctaactta tggacagtgg cgtaaagaat ttcaatcaag attcttccca gttcattatt
     6781 atggtctgaa ttttaatcaa ggaattgcgt tttcagattt agctatcctt tggagttatc
     6841 aacaactagc taagaaagta aaaaatttca agtttgatga ctattatgag attcgaacac
     6901 aggttattaa tgaggcagta aataatttta agaaatcgtc cctttctggt ctttttgatg
     6961 gtaaaattgg tacaatctgg ctcatttatg aatttgggga gatagatcgg gcagttgaac
     7021 tttttacaac tcattttata gagatatttg agaattcaca aaataaaaat ttatatagtg
     7081 ggcaggctgg aattttactg gttggactgt attttcttag taaaggggaa attgataata
     7141 aactaggaga agaaatacta attaggctta gagaatatac tttaaattat atagaaaatc
     7201 cagaaacttt ttgtaaagtt ggcgctagtg atgttcagag taatgatcct tatgagaatt
     7261 ttggtggatt actatatggt cacgcagggg tagcttggct cttcggtgaa gcttataaat
     7321 taactggtga aagtatctat aaaaatggtc tagaattggc tgtagataaa gaattagttg
     7381 cttataaagt agatagtaat aattcattgc agtacagcca aggtcataga ttactaccat
     7441 atctagcaac tggaagtgct ggcctgcttt tacttattaa tagaaataaa gaaatcttgt
     7501 caagcaaata cttaaaatat ttaacttctt tagagagggc aactgatgta gttttttgtg
     7561 tattaccagg tttatttaac ggattttgtg gtcttgaagt tgctaataat atatatagtg
     7621 atatcgatga taacttttca ggacaaaaga aattaattga acaactttat agatatttgt
     7681 gtgtaattga agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata
     7741 ttgcttcggg atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga
     7801 cgattcttcc tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca
     7861 gagtaatgaa atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc
     7921 ttcatcattt caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg
     7981 gtaccaattt gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga
     8041 agataatgac tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa
     8101 aattgtcttt gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag
     8161 gatttcatct gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt
     8221 aattggaata aaaaaatata ctatggaaga gttatttatc attgaagata tttcagtaga
     8281 aagttcgttc tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag
     8341 gccaagtaag ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga
     8401 aaacgaatgc tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa
     8461 gtcagtagac tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt
     8521 tagacactct tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac
     8581 agaaaaagca cagtactttc ttgggatctc ttcaactgaa gaaaagaaag atcaactaat
     8641 tctaagggaa acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc
     8701 tagaagagtt cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc
     8761 cgtcttttat cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat
     8821 aacatttgat gaaaagacaa cactttatga aaattcattt tctgatcgtg ttcttctgtt
     8881 tttagagagg agaacttttt acgaaaaaat acttagtttt gatttaatag aaaatacatt
     8941 aaaaactgaa tttgaaagac ccatcataaa atcgaataat actaaatatt tttctaaggt
     9001 gatttggact aaaaaagaca gttatgatgt aagcataccg atttctttat tttggaaaag
     9061 tgaagatacg gatgagctcc caagaagaaa aaaatgtatt ctaagtgtct atggggctta
     9121 tggtaagaat gataattctg atttagatga aattatgtta tctataatag atgcaggttt
     9181 tatttatgct atagttcatg taagaggtgg aggctatttg ggtggtgaat ggtatcgctc
     9241 aggaaaggcg ttaaacaaat ggaattctat cagagatttt attgagggag taaattattt
     9301 aagggaaaat gatgtaattg acagtaagcg attaggttta ataacttcta gtgcaggtgg
     9361 aataattgct ggtgcggtgt tgaacgagga aaaaaattta ttgcaaagca ttctcttatt
     9421 ttcgccattt ataaatcctt atgatacact tcagaatcca aatgatcctc tttctaagac
     9481 cgaaatagca gagtggggag atattagagc ccctgaagta aaggcttata taaagtcata
     9541 ttcgcctatg caaaatattg aaaaggcacg agactcaaat actgttatag ttaatatttt
     9601 gggtgagaaa gacccatata ttaataataa cgaagtgata gagtggtcaa agaaattaaa
     9661 ttctattgga gttaaaagtt tgttgtattt aaacaaagca gctgatcatg gaggttttac
     9721 tccatcagat gttctcttaa tgattgatac tttaaattat ttctttgaag aagtgggaag
     9781 gaataactta tgaattattt aaaatttata aaaaagacta aacttatatt aatggggatt
     9841 ttaatatttc tatcttcgtt taatggtgtc ttgctctcag gaattattgt atatgcaggt
     9901 agtttaaatc agacttcctc attttctgac gttctaagat ttggtgctat aagtattctg
     9961 ggttggtcag caatctatat atcaaattac tatttagaag taacggaagc atcaataacg
    10021 aaagatataa atgtaaaaat taaacaagga tattttagag aacagtatct ttcttctgaa
    10081 atggttaaag attactcatc tattatttca gttttgtcaa atgatttgag attaatcgaa
    10141 gaaaactatt ttagacaaat ttttgaaata atttcttcaa tattgttgtt catcgtctct
    10201 ctaagtttta tgctttattt aaacttccta gtttcaataa tatttattgt attatcagca
    10261 ttgccgatta tagtacctgt ctttatgaag aagatgttgt ctaattcagc taatgagtac
    10321 tctaacagca atgcagagta tactcacata ataaaagaaa tttttaatgg ttttaagaca
    10381 ttaaaatctt attctgttac taaagaaata attagtttgt cggataaaaa gttggataaa
    10441 ctagaagatt ctacttttaa tttgaaacga tcagaggttc tttcaaaatt ggttgcagta
    10501 ctaatttcag gtttttgctt tctagttccc ttggttgtag gatgttattt tgtaatttat
    10561 cataaaagcc tttcttttag tgaactgata ggtattttct tagcaaacga taaagtttta
    10621 gggccgatac aatcaattgc ctattcatta aataagataa atacaaccaa agatttaagg
    10681 aaaccgtttt taaaatactt aagtggagag aagaatttta tagacgctga acatgataat
    10741 aacggactgt atacttcatc aatagatgag atacacatga aagatgttgt atattctatt
    10801 acaccagaaa ataaattaag tattgacttc tcatttaagt caccatttag ggtattatta
    10861 acaggaactt ctggtagtgg gaaaacaacg attttaaatt taattaatgg ttctttaaag
    10921 ccacaaaaag gttatgtaaa tttgttatca catgggaaaa agagttcaga ttcaatacca
    10981 acagttgatc agacaccata tatttttgac actactattc gtgagaacgt aactttattt
    11041 caaaatgaat atttttcaga tgatcagata attgaggtgt taaaaaaggt aaatctatat
    11101 gaagaattag aaaagataga tatactaaat tatcaatgtg gtgaaaatgg tagtaatttg
    11161 tctggaggtc aaaaacaaaa aatagcttta gctagagctc tgattagaaa taataaagtg
    11221 tacttatttg acgaaatatc agctaattta gataatgata attcaaattc catacatgat
    11281 attctgttca atttaggtat ttcatttatt gaagtttcac atcattatga cttaaatgac
    11341 aagagataca ctgatatata taaattggaa aatggaacac ttttcaaaat taagtgattg
    11401 gaggcaagaa tatggataaa gattatatat taaaagtgaa agggctgtat catcaatttt
    11461 tactaggaaa taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct gcttcgagag
    11521 gagaatttat aagtattcta ggaattagtg gttctggaaa gtcaacttta ttaaaatgta
    11581 tttctagttt gcttgaacca acaagtgggg aagtaatttt aaatggaatc aacccctata
    11641 aaatcagaaa tgcaaaattg tcaagtataa gacgtaacga agtatctttt atatttcaag
    11701 catacaattt aataccttcc ctgccggtaa tagaaaatat agcacttcct ttgcgattat
    11761 cacaaaaaaa attaactatt aaaaatgtag aaaacttact caaaagaatg aagtttaatg
    11821 ctggcttaaa cgattttgtt ggaactctgt ctggtggaga acaacagaag gttgctatag
    11881 ctagagcggt tattgctgat agtgatataa tatttgctga tgagccaact ggggctttag
    11941 acagcgtttc tcgtgaagta atttttgaat tattgagaga gttagtaggg gcgggtaagt
    12001 gtgtaattat ggtaacgcac gatatagaat tggcctcgaa aactgatcgt gcattaatat
    12061 tgaaagatgg aaaaattttc aaagaacttc atagacctag cggggaagag ttgtataaaa
    12121 tcttagaggt acaatcaact acggaggaat agagatgctc agattagttt actatcaatt
    12181 tttacataat aagaaacaat ggttaggcgt ttcaccagta atttttgttt cgagtttagt
    12241 tatgggattg gctgttaatg gggtaattaa tgttgagaat aattcgcaag tatttgtcgg
    12301 acttccagat cctaaaccaa tttttatgtt tccgatagta ttcggaggag taacattatt
    12361 ctttgtgctc tctaacataa taaatatgtt ggttgaaatt tttagagatg attacgaact
    12421 tctggaggtg ttgggagcaa gtctccttca actatcattt cttgtggggg gacagatttt
    12481 tatcatttcc tcgattatta gttttattgc atatctatgc tctatatttg ttacgtcaaa
    12541 ttattattat tttttacaat actttttcgg ggaaaatata ttacctgata tccaatttca
    12601 aacgagtgct gttggatgta tcattacagt agtgttgatt tcatttttag cctttctaag
    12661 tggttgcttt tatacgttca agaagattag aaataggaag agttctaaaa taaggcatgt
    12721 tctttcaata gtaaaaagaa tactgctatt ggctggtttt agtgttatct ggttgctttc
    12781 tttgcaacaa attttccaag atagcacaat tttagcgaag gcccagataa tattcaatat
    12841 tgtaatctta gatattgtga ttatttatca attatctcct tttatacagt catgttttat
    12901 caaactactt tctataatta tatttagaaa taattttatg ttcattgttt cgaagtggaa
    12961 tttactatat cgtaagcctt atataaaatc tatcagtgct gcaattacag gagctatatt
    13021 actaatttct agttttcaaa tgatttctca gaatatacta tctcaatttc aagatgacag
    13081 tgatttagag ctaaaagtgg catttattgt ctatgtagga gcaccaattt tgatagtact
    13141 tgcaaatatt atttcaattg catttttgtc atcgcatcaa gaaaggattg agattcagca
    13201 atttgaaatt ttaggaactt ctaattacca gatggttaag attaaagttg gtgaggctat
    13261 atttttgaca tttgttactt cattaattgc atttttactt aacattaaaa ttatggcttt
    13321 aatatactac tctttagagg atattttaat agatgatatg aatcttttgg ggttaatact
    13381 tccaaatttt atagtgtcta ttattttatt tattttaata tttattacta aaagttccta
    13441 ttttatcttt aagaatgcaa aaattatttc ttaaaaattt gtaagttttc gctatataaa
    13501 atagttaaat ctattttgta tcattgcttg cattttactg gtggagagaa tataatgagg
    13561 gtggataaag tacttaat
//
LOCUS       DBEJAQ010000046        13401 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.046, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000046 DBEJAQ010000000
VERSION     DBEJAQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.046"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(409..1278)
                     /gene="gla"
                     /locus_tag="ACL297_001637"
     CDS             complement(409..1278)
                     /gene="gla"
                     /locus_tag="ACL297_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HGR0071871.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1502..2023)
                     /locus_tag="ACL297_001638"
     CDS             complement(1502..2023)
                     /locus_tag="ACL297_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGR0071872.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(1981..3087)
                     /locus_tag="ACL297_001639"
     CDS             complement(1981..3087)
                     /locus_tag="ACL297_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HGR0071873.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3198..3452)
                     /locus_tag="ACL297_001640"
     CDS             complement(3198..3452)
                     /locus_tag="ACL297_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071874.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(3792..4637)
                     /locus_tag="ACL297_001641"
                     /pseudo
     CDS             complement(3792..4637)
                     /locus_tag="ACL297_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078372828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            4871..6589
                     /locus_tag="ACL297_001642"
     CDS             4871..6589
                     /locus_tag="ACL297_001642"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGR0071875.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            6700..7047
                     /locus_tag="ACL297_001643"
     CDS             6700..7047
                     /locus_tag="ACL297_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGR0071876.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7326..8162)
                     /locus_tag="ACL297_001644"
     CDS             complement(7326..8162)
                     /locus_tag="ACL297_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0071877.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8175..8804)
                     /locus_tag="ACL297_001645"
     CDS             complement(8175..8804)
                     /locus_tag="ACL297_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGR0071878.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(8814..9455)
                     /locus_tag="ACL297_001646"
     CDS             complement(8814..9455)
                     /locus_tag="ACL297_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGR0071879.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(9601..10830)
                     /locus_tag="ACL297_001647"
     CDS             complement(9601..10830)
                     /locus_tag="ACL297_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGR0071880.1"
                     /translation="MNNSQQMLQALEEQDLTKAEYYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLMLGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(10820..11986)
                     /locus_tag="ACL297_001648"
     CDS             complement(10820..11986)
                     /locus_tag="ACL297_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGR0071881.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVIFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12130..13143
                     /locus_tag="ACL297_001649"
     CDS             12130..13143
                     /locus_tag="ACL297_001649"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HGR0071882.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNEFHVPEAVCIR
                     FAP"
BASE COUNT         4085 a         2886 c         2363 g         4067 t
ORIGIN      
        1 atagcaaaat gaaatagaaa ctgaacattt tgttgaggaa ggtaaatcaa ttcctcacaa
       61 tgtttgagaa agaagggagg actattccaa cttcaaccca ctataaagca gacttacaag
      121 atagggaacc catctccgat aatatatagt agaatgaaaa ctgcacaatt tgattggaga
      181 attcaaacca atttctcaca atgttttaga aacaagggtc tattatttca actgcaacct
      241 actatagtcc attgaagctg gaatagccca ctgagacttc taaaccactg ctggagagga
      301 gtttgtttag agtttctttc atttccctaa aaaatcaaaa agagagaacc agtctgatcc
      361 tctcttattt atagatggtg ctcactcaaa tgttaaagga gctatttctt agagataaag
      421 gaatttgaat acagctactg ccgcaattgc tgctacgata ggtgctacta ctggtaccca
      481 agaataccac cattttgaat cgcctttatg ctcaccaaga actgattttg gaaggaaagc
      541 atgaaggaga cgtggtccca agtcacgggc tgggttcaag gcaggtcctg taggtcctcc
      601 aagtgatgtt accaaagcca taacgaggaa tccaagtgcc aagtgagcca cagaaagtcc
      661 tgaagcagtg tgtggagcca cctgtgcttt aatagccaag tcagaaaaat caactgtttg
      721 tcctgcttct attgcctttt gtttcatgaa ttgaagcact tcagcaccaa agaagttttt
      781 agtcaaacca agagctgcaa agaacaaaac aaatgaacca acaaactcat taatcaaacc
      841 attgacagtt gctgcatagc gactttcttt tgtaccatgg tcaatacttg aaatagttga
      901 gaaagttccc aagatgttat ttgggttttc agttttcaag tagaatggac ggtatgttgc
      961 cacaactaag gcttggccaa agatagctcc caagacttgc gcgataatat aaggtaccac
     1021 ttgtgcccaa gggaaaagac cgctaactgc aagccctaga gtgaaagcag ggttgatgtg
     1081 attcccagat acgttaccaa acatcaaggc tgggatcata acccccatac cataaccaac
     1141 agcgatgacg atccagccac tttggtgacc tttcgtacct ttaagttcaa cgttggcaac
     1201 tgcaccattc ccaagaatga tcaaaatggc agttcccaaa aattcagtgg catacttcag
     1261 tgcccatgtg aaatccattt gctagattct ccttaaaatt ttttagatat tccttatttt
     1321 atcaattttt accctattta gcaactgatt tctggaaata tttttaaaaa aataggattc
     1381 atagtttccc agttcaacgg acttgtaagc gtttttattc ttcacgaaag acctcgttct
     1441 caaaaccagg aaaaaagccc acatctgtga gcttttcctg gttcctagat tctaaaatct
     1501 attatttttc atcttctttt ttcttgaaga atcctaatcc tagactagct aacaaggtca
     1561 tgataccagc actagccaag gtagcattag cttctgtacc tgtgtttggc aattcctctc
     1621 tcttacctgt ctcataagtc ggaacttctg ggtctggatt cactggagtt tcagtttttg
     1681 gagtacctgg gtctggagtt ggtttatctg gtgttgataa acggtcatac cttaccgtta
     1741 tttctttatc actagagtct gacgtaactt cttgtgattc aactgttgga atatctggat
     1801 ctttgtactt gtcaatctta ccagatacaa cctcgtccca gtttcctgtt gtccattcac
     1861 cgtaggttac aactcccgtg accttgttct cagtttttgt acggcttaag gttacaggtt
     1921 gaacaacatc ttcttttaca ttttggttcg taactttatc aacgtaatga atgatacgcg
     1981 ttatagtctt cgtctcagta gaggttgctg ttttgggaac cactgtttcc tcaacattct
     2041 cacggtagta atagtcaact gttgcaccgt cttctggtac gcatttgcag gagttgctac
     2101 caaggtgtat gttgttttcc ttgtgataac tcggtcttct ttgtcctcag ttgttgtttt
     2161 cccttcaata gtttttgatt ctgtggtata ctcagaacct atcgctaaat cagcttttat
     2221 aacagactct gccaacttct cttggctacc ttctttatag taattggctg ttactgtagc
     2281 agtggttggc gcttcgcttt ctctataaac taaggtcact gttctacctt cgcttacaat
     2341 attcccagtt aaacttgcag aatttgtatc tgcttcttta aaagtataat attttccgtc
     2401 agtagtagtc atgctactga gttttttatc tgtgacattg tagctggtac caatcagttg
     2461 ttttttattg gtaatgtagg ttccgtcact ttctttttct ccaattccag tatcattttt
     2521 caatgatagc aaacgccctt gttcatcaac atagcgaact ttcacatttt ctgagattag
     2581 ttctgccaat tctgaggttt ttttcttttt cttgatttct tcggttattt tccctttctc
     2641 ttcttcggga atatttagtt ttggaatgat tttttcaaca acggttcgtg atggttccac
     2701 agtatcttgg atgactgaaa agtcagctag aattgggaga ttatactgaa cacggtgact
     2761 ttgagtgttt actcctactc tttcattatt ctctgaaaat acttgtacgg tataagaaac
     2821 aacatctttt cctaatagaa catccccagt agagaaatag ccgccttttc ctagtttgct
     2881 atctccagag cccacttctt tcctaatctt atcagatagt tttttaccag tcagtacatt
     2941 cgttcgcaca atccctttgt ctacccctac aaagtgggag aactttttga actcttcaga
     3001 accagatcta gcccaaccat tattaagggc atttgctttt gtatttgtat tctctctcaa
     3061 aagtttggcg attagaatta tattcatcgg cacttagagt tgctgctata tctgactctt
     3121 gaataccaac ttccttacta ccatttctag cggcagtata tgtgtattta tttgtttcta
     3181 cttctagctg attatcctta acatgatagg taattttctc ctggtataag agtcttcagt
     3241 agtttatatg ctctgtcatc atctttagct ttagaagcag ctgagtttag ccaagttggg
     3301 gtattaggag tatctttatc agcctgtttc aagacattat tcaagtcatt tgctagccta
     3361 ttggttgtcc tcactgtgaa agttttcttc cagtctgaac cacttttatt agacttagat
     3421 atgctcatga aatcctggtc aacattcaac acaatatcgc cttgtttttt agcatcttcc
     3481 gttgcttctg cttcaaattg atcatggagt ttttgttgct ctggtgtata gtttttttat
     3541 ttacacgatt taatgtacta ttttttattt tagtattaag aactccatta atacgatttt
     3601 taaactctgc ttcaaaaaat cccttaattt gttggtgcgc aagttcaaca ttcaaacctt
     3661 tcttatacaa gtcttcattc taaacgattt cggctacacg gtgaatggtt tgattgtata
     3721 ccgtcttctc ttaaccagca gtatccttag ttttaatgac tactcgatag gttcctttag
     3781 aaccgttata gtcatctagt tttaaaaaag cattccaaac attcaaaaat agttggaact
     3841 tcccttaata aatccgtcac tttctttttc aagatagccc aagcttgctc aataggattc
     3901 aaatccggtg aataaggtgg tagaggtaag aaaaagtgtt tatcttggat gcagagttca
     3961 tccaaaatgt tcttgggatg aaaactagca ttgcccatga caataacatg aggtgtcttc
     4021 aaagcaggta ggagttgcgt tttgaaccac tccacaaaga aatcgcttgt catgcttttc
     4081 ttgtaaatca tgggagctat aaactctccg tctacttgtc ctgcaacaat tgaagttcgc
     4141 tcaaaacgac gtccgctaat ctttttatag actttctccc ctctaggagc ccgtgcataa
     4201 ggacgataga ggtagcggtc gattcctgtt tcatcaatat atacgattgg taaatctttt
     4261 aggttatcca aaatatcaag aaactcagcg actttctctg gctcttgttc cttaaagctc
     4321 gtcatctttt tttaaagtga ccttaatata ctttaaagct gcccatacgg agggcagagc
     4381 acaatcaaaa tgtgccgcaa tttcccgtaa aaaagcatct ggatgagcct ctacaaaggc
     4441 tttcaattcc tctaaaggaa tctttcggtt tttgacgact cgctttttcc tctctatgtg
     4501 tccttgttca catctttctt ttcccacgtg aagagagttc tgacgccaac accaaaaaac
     4561 ttggctgcct cgacatggct gtgcccctct ttgatgtaat ctaatgctcg ctgtttaaaa
     4621 tcagtactat atgccatagc tttattataa tatgcttgtt taaaactaag caactatatc
     4681 aatgttttgt tattagaaga cttacttttt ttactgaaat cgaatttttc ctaacctcaa
     4741 catcatataa ccccaaaatt caatctgacc tggtttgaag agatgttcga agcagataag
     4801 gttttactaa aactaaaaaa aatgatataa tcaaactgat taaatgtttt aaaaataaag
     4861 gagaatttga atgtcttacc aagaaaatta ccagaaatgg gttgattttg tggagcttcc
     4921 tgactacctt cgtcaagatt tggaaaatat ggacgaaaaa actaaggaag atgccttcta
     4981 tacaaatctt gaatttggta ctgcaggtat gcgtggcttg gttggtgctg gtacaaaccg
     5041 tatcaacatc tacgttgttc gccaagctac tgaaggattg gctcgtttga ttgagtcaaa
     5101 aggtggaaac gagaaagaac gcggtgtagc aattgcctac gatagccgtc acttctcacc
     5161 tgagtttgcc tttgaatctg cggcagttct tgctaaacac ggtatcaaat cttacgtatt
     5221 tgaaagcctt cgtccgactc cagaactatc atttgcagtt cgtcatctca actgtttcgc
     5281 aggtatcatg gtcacagcca gccacaaccc tgctccattt aacggttaca aggtttacgg
     5341 tgaagacggt ggacaaatgc ctccacacga tgcggacgct ttgactactt atatccgtgc
     5401 aatcgaaaac ccatttgcag ttgaagttgc tgatgtggaa actgaaaaag cttctggctt
     5461 gattgaagtt atcggcgaag ctgttgacgt agaatacctt aaagaggtta aggacgtaaa
     5521 catcaaccca gccttgattg aagaatttgg taaagacatg aagattgtct acacaccact
     5581 tcatggtact ggtgaaatgt tggctcgtcg tgctcttgcc caagcaggat ttgactctgt
     5641 tcaagttgtt gaagcgcaag caactgctga cccagacttc tcaactgtaa catctccaaa
     5701 cccagaaagc caagcagcct ttgcccttgc tgaagaactt ggtcgtcaag ttggtgcaga
     5761 tgttcttgtc gcaactgacc cagacgctga ccgtgttggt gttgaagttc ttcaaaaaga
     5821 tggtagctac ctcaaccttt caggtaacca aatcggtgct atcatggcta aatacatctt
     5881 ggaagctcac aaaaacgctg gaactcttcc tgaaaatgcc gctctctgca aatctatcgt
     5941 ttcaactgac ttggtaacga agattgctga aagctacggc gcaactatgt tcaacgtctt
     6001 gacaggtttc aaatttatcg ctgaaaaaat ccaagaattc gaagaaaaac acaaccacac
     6061 ttacatgatg ggatttgaag aaagcttcgg ttacttgatt aaaccatttg tacgtgataa
     6121 agatgccatc caagccgttc ttgtcgttgc tgaacttgct gcctactacc gttctcgtgg
     6181 tttgacactt gctgacggta tcgaagaaat ctataaagaa tatggctact acgcagaaaa
     6241 aacaatctct gttactcttt caggtgtcga tggtgctgaa caaatcaaag cgattatggc
     6301 taaattccgc aacaatgctc caacagaatg gaacgcaaca gctatcactg tcgtagaaga
     6361 cttcaaggca caaactgcta ctgttgctga cggtactgtt acaaacttga caactcctcc
     6421 aagtgatgtg ttgaaataca cacttgctga cggttcatgg attgccgttc gcccttcagg
     6481 tacagaacca aaaatcaagt tctacattgc agttgtaggg gaaaccaacg aagaatcaca
     6541 agctaagatt actaacatcg aagcagaaat caatgcattt gtaaaataaa gacctataaa
     6601 agatgagacc aaccaacctc atcttttttc gtatcaaatc taagcaattt tagaaacttt
     6661 atggcatact agacttatca atgaaagcga ggtaatctca tggaacaatt tttagataac
     6721 atcaaagacc ttgaagtcac tacagttgtg cgtgcgcaag aagctcttga taaaaaagaa
     6781 actgcaacct tctttatcgg tcgcaaaact tgcccttact gccgtaaatt tgcaggtaca
     6841 ttgtcaggtg tcgtagctga aaccaaagct cacatttact tcatcaatag tgaagaacca
     6901 agccaactca atgatttgca agcattccgc tcacgttatg gaatcccaac tgtaccaggt
     6961 tttattcata ttacagatgg acaaatcaac gtccgttgcg actcttcaat gtcagcacaa
     7021 gaaatcaaag atttcgcagg attgtaaaaa atcagctaac aagtcttaaa atcacaaaaa
     7081 cgcataatgt aaggtgcaaa aaccttgata ttatgcgttt tattgttcct aaaacagtta
     7141 catcagcatt tcattacaga acattttaat tttggaagtc aaatcaattt ttaacaatgt
     7201 tttagaaatc acagtgggct attccatatt caattatcca gattcaatcg ctattatcgt
     7261 tttcctagat tacaaaaaaa actacgtttt gctaatcaaa acgtagtctc agccactatt
     7321 ttatcttact gtccttcttt tacttctttg gttgctacat cttctccaaa ccatttttgg
     7381 ctgatttctt ggaacttgcc gtccttgtaa agactagaaa aagcttcatt tatcttctta
     7441 accaaggttg tatcttcctt acgggctcca accgcaaaag cttctgtttc tagtccaact
     7501 gtaaagacat tataatcgtt taaaacacct tctgcttcta aataatagtt tgcatagaca
     7561 cggtcaatca atagaccatc aattcgatcg tttttcaaat caatcaaggc ttcattaaag
     7621 gtttggtatt gattcgcttc cttattagcg acaatattct tcaaaatttc tggatttgct
     7681 tcaaagtccg cataaccaga tgaaccagct tgagctccta atgtctttcc agtcatatcc
     7741 tttgcagtcg tgataccaga tgattttttc gtaaccaata cctgctcatt cttcatatat
     7801 gagttactga aagccacctt ttcacggcgt tcgtctgtag cggaatagcc attccaaatc
     7861 agatcaatcg ttccttttgt caattcagct tctttcaaat cccaatcaat cggttgccaa
     7921 tttaccgtga ttccgtattt ttcaaaaaca gctgtagcta aatcaatatc aaatcctgca
     7981 taagaaccat ctttctgagc aaatcccatt ggaacaaaag tactatcaaa tccaatagta
     8041 atagacttgt tagactggta ctttgaccaa ttatctccac tagtttcgct agaatttttc
     8101 ccacaagcta ctaagaacaa agcagtcatc agactgacta atacaagcat ccattttttc
     8161 atccattttt cctcctattt aggttctact ttcaataata catctgcgat attttcagca
     8221 aactgcaaat catgggtaac cacaatctgg gtcatcccaa gttccctatt ttgcaagatt
     8281 agcttctcca cttccaaacg taattctgga tccagggcag aagttggttc atcgtagcca
     8341 atgatttctg ggtcaatcat catagcacgc gccaaagcca cccgctgctt ttgcccacca
     8401 gatagtgaga aaggataggc ctctgcgtgt cctcctagtc ctaactgttc caagagtcca
     8461 ctcgccttct tctcagcctc ttcctgcttc attcccatgg tcttcacagg cgataaagtc
     8521 aaattttcca gaactgatag atgaggaaat agttgaaaat cttggaagac aaatcctagt
     8581 aggttgcgct tctgcaattc atccagctct aaaggttgtc cattataaaa gatttgccct
     8641 gaatcaatgg tttcaagacc cgcaagcata cgtaaaagag tcgtcttacc tccaccagaa
     8701 ggtccaacga tagccaggat ttgcttttca ggaatactta gactgaaatt agacaggatt
     8761 tgtttgtctc caaagacttt attgatattt cgtaattcta acatggcagc ctcctatcta
     8821 taataactat acttcttctc aacttttttg gaaataattg tcacaatccc aatcaaaatc
     8881 aaataaatgg ctcctgccaa gaacatagga actagactag catcgcggtt agcagctgtt
     8941 cgactagcca agataaggtc tgaaattccg agagcataga ccaaagaagt atccttgacc
     9001 aaactcataa cttcattaaa gacactagga agaacgatct tggtcacttg gggcaagata
     9061 atatagcgca ctctgtcaaa agggctaaac ttcaagacct tggcggcctc atactgtcct
     9121 cttggaatag tgtcaattcc cccacggaaa atttctgcaa agtaagctgc atagttgaga
     9181 acaaaggcaa taatagctgc aggaaggcgg tctaaacgaa tcccaatact tgggagcaca
     9241 taatagataa aaattagttg caagagtaag ggggttcctc gcataaccca gatgtaaatg
     9301 ttaatcagat aatggagggg cttccaatgg acttgcaagg caaaagcaat caaaacgccc
     9361 aagggaatcg aaaatagcaa gaccagtgca aagacctgta cagtcgtgct cgcaccattc
     9421 agtaaactcg gtaatatctc aaataaataa gacatactgc acctcctaaa agtaattgtt
     9481 cctattatag catgaataaa caagattacc aagtattttt gtaaaaagat tctaccatat
     9541 acaaataaaa accgtagcta tcaaaagaga taaactacag ttctatatgt ttaaacattc
     9601 ttacaatctc tcaaacagtt cttgcatttg cacatcatct ggaaccagtt ttaagtaagt
     9661 gtgagcatgg acttttgctt cttcaaaatg tcccaattca cgcaagagat agatatagtg
     9721 ttccagaaat tctggattgt ccttcaaatc tcctgtcaac tcttgataat gctcataagc
     9781 agtatccaaa tcgtccattt cttgataaga acgagcaatc atccacttgg tcaaaagatt
     9841 ttctggctcc tcactctgca agtctagaat atcctcataa cgctcctgct ccagataaat
     9901 agtggctaaa cgaagcaaga tttcttctgt atcctcagcg tcttcttttg cagcaagaag
     9961 ataattttct gcaccactag catcatgcaa ttcataagaa aattgtgaag cagctagcaa
    10021 gaggcgagtt tcaaagggat ttttctctaa tccttgctta gcgatacgca gggcttcttg
    10081 aacttgatgt tccttatgta aagcctgact gtacccatac tcatagcctt caaagtcagg
    10141 agaaatggta tcaagctgct taaagtagag ggtggctttt tgatattctt cttgatcaaa
    10201 ataaagactg gccaactcaa aagctgttaa gtcatcgtat tctaactcca gggctttttc
    10261 taaaaactca gtagccgttt caaatttccc taactgagca taggcaaagc caattcgttg
    10321 ataggtggaa atgcccgttt gctcataaat cgagcgatta tctaactggg cataggcttg
    10381 aatagccgct tggtaatttt ccaactcact atccaactct gccaaaccca acatcaagag
    10441 agaatcctct gagtaggtca aggcctccaa taatttctca cgtgccacat ctgtcaaacc
    10501 ttccagctgg taaaggtctg ccttcaaaac caaagacgag acataccagt cactgtcagc
    10561 ttggatttcc tcaagatagg taaaggcttc ttctatttga ccatcctcgc tagcaattgc
    10621 agctagatta agatgaacct ctggaaaatc ctctacaatt ttcaggtaaa tttccttggc
    10681 ctgaggatag aaaccaatcc cttcaagata agttgccaac tcatacagaa gatcacttga
    10741 atcattttct aaagctttgg cgaaataata ctcagcctta gttaaatctt gctcctccaa
    10801 agcctgtaac atctgttgac tattgttcac tcttgacttc ctcaccctct aattcatata
    10861 gaccactgac taccttatac cattcgaaaa tggctgaaat gacaacctta gcagaggcat
    10921 aaaccggaat accaagtaaa actccccaga taccaaacat agatcctgaa gttaacaaaa
    10981 caaagagaac attaataggg tggatgttta attgacttcc caaaatcaat ggagagacaa
    11041 aacggccttc aatagtttgt tctacgataa agacaatcac tactttcaaa agcatgactg
    11101 gaccagcaat caaacccaat actagagcag gaagcatggc tagaaagcta ccaagataag
    11161 ggaccagatt taaaatacca gcagtaaccc ccagcgtaac cgcatagcgt agaccaataa
    11221 tcttgaagaa gatgataaat attactgcta caataatagc cactgtcact tgccctcgaa
    11281 catagttgga caactgttga ttcacatctg ataaaacttg tccaacaggt tccttcaatt
    11341 ttcttggaat gaattgggtc aaatagttac gcaagccttt cccatcacgc aagagataaa
    11401 agagcatgaa aggaacgata atcaaggcaa caatcacttg agaagctcca ctaataaagg
    11461 cactcaccca gttgactgcc tgagatgaaa ccttacttgc caaaactgta gcctggctag
    11521 aaaaattggt caaaacttgc tctaattgag gtctgaaatc atctggcagg tgctgggcta
    11581 ccaatccatt aacaatccta tctatatctt ctaagtaaac aggaacgttt cttgcaaagg
    11641 tcaaaacctg acgttgcaga tttggaatgg cgactgccaa gccccaaatg ataaagagag
    11701 cgatgataac aaagacaata gtgatagcta taacacgatt aaccttatgc ttctccatcc
    11761 aatcaacaat aggattcaac aaataatata acaaaccaga caaaatgact ggcaacatca
    11821 caactgctaa aaaatctaaa acaggtgaaa atagaaaact aatcttactt aaaataaaaa
    11881 gattcaatcc caataataag gttactaaaa ataccgtaat tgccttgtta tctaaaaacc
    11941 acttgaaaaa ccaagatagg ctaaaatgtt tctctttttg ctccataaat acttcctttc
    12001 tattgttctc tcattatacc atttttgaaa caaaataact cttttttaaa gtgcttacat
    12061 ctaattgaat gtctttaatt tgcttgtttt tgtgatataa tagtcttatc tttacacaga
    12121 ggtatagtaa tgaaagaaac tgtttatttt ggaacttata cacgtcgtac ttctcaaggg
    12181 atttacaagg cagactttga tacagaaact ggtcagcttt caaatctaga actttttgca
    12241 gctgagccaa gtccaaccta ccttgccttt gaccagcacc aacatttata cactgttggt
    12301 agccaagacg ataagggggg aattgcagcc tatcaaactg acgggactgt gttaaatcat
    12361 gttgttgaag aaggagctcc ccactgttat gttgctgtcg atgaaaagcg tgatttggtt
    12421 tacgcagcca actatcacaa gggacaagtc cttgtttata aacgccagga agatggtagt
    12481 cttctactta gtgatatgga tcaacacagt ggccaaggtc cacatgaaaa tcaagcttcc
    12541 ccccatgttc actatacaga tttaacacct gaccactatc tagtgacctg cgacttggga
    12601 actgaccaag tcatcaccta tgacctcgat caagaaggaa aattatctaa gctctatacc
    12661 tatcacagca agccaggagc aggctcacgc catatcattt tccataacca ctataaaatc
    12721 gcttatctca tttgtgaact caatagtact atcgaagttt taatctacga tggcgttggc
    12781 gaatttgtac gtatgcaggt tatttcaact ttaccagaag cttacgaagg ctttaatgga
    12841 accgctgcta ttcatctctc taaagacggt aaatacctct acgcttctaa ccgtggccat
    12901 gattctatcg cagtatatac catccttgcg gacggtagct tagagttatt agaaatcgtt
    12961 ccaacgcatg gtcagactcc acgtgatttt gatttgacac ccgaccaaaa atttctcatt
    13021 gttgtccatc aagactctga caatgcaact gtctttaaac gtaattgtga caatggtcgt
    13081 ctagcagaac tctccaacga attccatgtt cccgaagcag tctgcatccg ttttgctcct
    13141 taaaacacca taaaaaatgg ctctataata tttgtagtgg gtaaatcccc tatggatatt
    13201 atggagccta tttttgtgta gaaaaaaagt cccataagat ctataatgaa aagcgatcaa
    13261 acaactcatt agaaagattc atatggaaca attacatttt atcacaaaac tactcgatat
    13321 caaagacaca aatatccaaa ttatagatgt cgtcaatagg gattcacaca aagaaatcat
    13381 cgccaaactg gactacgacg c
//
LOCUS       DBEJAQ010000047        13037 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.047, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000047 DBEJAQ010000000
VERSION     DBEJAQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.047"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            173..1456
                     /locus_tag="ACL297_001650"
     CDS             173..1456
                     /locus_tag="ACL297_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGR0071883.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(1651..3222)
                     /gene="ffh"
                     /locus_tag="ACL297_001651"
     CDS             complement(1651..3222)
                     /gene="ffh"
                     /locus_tag="ACL297_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGR0071884.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(3234..3566)
                     /locus_tag="ACL297_001652"
     CDS             complement(3234..3566)
                     /locus_tag="ACL297_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HGR0071885.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            complement(3657..4034)
                     /locus_tag="ACL297_001653"
     CDS             complement(3657..4034)
                     /locus_tag="ACL297_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGR0071886.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            4106..5410
                     /locus_tag="ACL297_001654"
     CDS             4106..5410
                     /locus_tag="ACL297_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGR0071887.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            5423..6229
                     /gene="yidA"
                     /locus_tag="ACL297_001655"
     CDS             5423..6229
                     /gene="yidA"
                     /locus_tag="ACL297_001655"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGR0071888.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            6272..7270
                     /locus_tag="ACL297_001656"
     CDS             6272..7270
                     /locus_tag="ACL297_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003712 - transcription coregulator
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HGR0071889.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNDLECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(7436..7507)
                     /locus_tag="ACL297_001657"
     tRNA            complement(7436..7507)
                     /locus_tag="ACL297_001657"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7472..7474),aa:Arg,seq:cct)
     gene            complement(7549..7896)
                     /gene="rplS"
                     /locus_tag="ACL297_001658"
     CDS             complement(7549..7896)
                     /gene="rplS"
                     /locus_tag="ACL297_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGR0071890.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(8015..8344)
                     /gene="crcB"
                     /locus_tag="ACL297_001659"
     CDS             complement(8015..8344)
                     /gene="crcB"
                     /locus_tag="ACL297_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGR0071891.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(8338..8712)
                     /gene="crcB"
                     /locus_tag="ACL297_001660"
     CDS             complement(8338..8712)
                     /gene="crcB"
                     /locus_tag="ACL297_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGR0071892.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(8709..8975)
                     /locus_tag="ACL297_001661"
     CDS             complement(8709..8975)
                     /locus_tag="ACL297_001661"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HGR0071893.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(9091..9534)
                     /locus_tag="ACL297_001662"
     CDS             complement(9091..9534)
                     /locus_tag="ACL297_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGR0071894.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            9638..10573
                     /locus_tag="ACL297_001663"
     CDS             9638..10573
                     /locus_tag="ACL297_001663"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HGR0071895.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            10669..10911
                     /locus_tag="ACL297_001664"
     CDS             10669..10911
                     /locus_tag="ACL297_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGR0071896.1"
                     /translation="MKKDIHPEYRPVVFMDTTTGYQFLSGSTKRSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(10968..11546)
                     /locus_tag="ACL297_001665"
     CDS             complement(10968..11546)
                     /locus_tag="ACL297_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HGR0071897.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKT
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSTAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(11521..12534)
                     /gene="efeB"
                     /locus_tag="ACL297_001666"
                     /pseudo
     CDS             complement(11521..12534)
                     /gene="efeB"
                     /locus_tag="ACL297_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
     gene            complement(12537..12996)
                     /locus_tag="ACL297_001667"
                     /pseudo
     CDS             complement(12537..12996)
                     /locus_tag="ACL297_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         3916 a         2913 c         2331 g         3877 t
ORIGIN      
        1 ttttttctgc tatactatcc taagcaaagg tttttaatgt catcccgtga ggtgacgaag
       61 acgcagaaat atttgaaact ctttaaagtc tagattttag agaagtctta ctctgagggc
      121 ctattgctgt aaaataatgg gctcttttct gatgcccaaa agtgaggttt atatgaaaca
      181 agaatcaact gttgatttgt tactagacgt tgaccaacgt ccttcggatg gaaaaggaat
      241 tctccttagc ttccaacacg ttttcgccat gtttggtgcg accatcttgg taccattgat
      301 tttgggaatg cctgtatctg ttgccctttt tgcttcaggt gttggaacac tcatctacat
      361 gattgctact ggttttaaag ttccagttta tctaggttct tcatttgcct ttatcacagc
      421 tatgtcactg gctatgaaag aaatgggggg ggatgtatct gctgcccaaa caggggttat
      481 cttgactggt ttggtctatg tccttgttgc taccagcatc cgatttgtag gaacaaaatg
      541 gattgataaa ctcttgccac caatcattat cggtcctatg atcatcgtta tcggtcttgg
      601 acttgcaggt tcagctgtta ccaatgcagg tcttgtagca gacggaaatt ggaaaaatgc
      661 tctggtagcc gttgttactt tcctaattgc tgcctttatc aatacaaaag gaaaaggctt
      721 cctacgaatc attccattcc tctttgccat tatcggtggt taccttttcg cactaactct
      781 tggcttggtt gactttacac cagttcttaa agccaactgg ttcgaaattc ctggtttcta
      841 cttgccattt agcacaggtg gtgcctttaa agagtacaat ctttactttg gtccagaagc
      901 catcgctatc ttgccaatcg ctatcgtaac aatttctgaa catatcggag accatactgt
      961 tttgggtcaa atctgtggcc gtcaattctt aaaagaacca ggtcttcacc gtactcttat
     1021 tggtgacggt atcgcaactt ctgtttctgc cttccttggt ggaccagcca atacaactta
     1081 cggagaaaat acaggggtta tcggtatgac tcgtatcgct tctgtctcag ttatccgtaa
     1141 cgctgccttc atcgcgattg ccctcagctt ccttggtaaa ttcactgcct tgatttcaac
     1201 tattccaaac gctgtacttg gtggtatgtc aatccttctc tatggggtta tcgccagcaa
     1261 tggtttgaaa gtcttgatta aagaacgtgt tgatttcgct caaatgcgaa acctcatcat
     1321 cgcaagtgct atgttggttc ttggacttgg aggagctatc cttaaacttg gtccagttac
     1381 actttcaggt actgcccttt cagccatgac aggaatcatc ttgaacttga tcttgccata
     1441 cgaaaataaa gactaagagt ctaaatacac ctaatccact cagacagctg agtggatttt
     1501 tcgtatatca taataaaagt gtcttaacaa aattattaaa atcaaaaaac gtataatatc
     1561 agatattcta aaaccttgat actgtacgtt ttatcataga aatttttact ttattttctc
     1621 atcaaatgag atttgcatca atctcttgtc ttacttgcgt ttcttcttcg ctttcttcat
     1681 tttgttagcc atacgtttca tggactgttt catggcaaat tcaccaattt tacctttcaa
     1741 accgccacca aacatctggc tcatatctgg cattcctgct cctccgagag ctgataagtc
     1801 aggcataccg ccttgtccca tcattccttc aagggcagac atatccattc ctcccatatt
     1861 tggcatattt ttaggaaggt tatttggatt aatccccatt tgcttcatca ttttattcat
     1921 atccccagac ataacaccct gcatgagctg tttagcctgg ttaaagtcct tgataaattt
     1981 attgacttcg acgaatgtat ttccagaacc agcagcaata cgacggcgac ggcttggatt
     2041 taacaaatct gggttttcac gttcttcagg tgtcatcgaa gacacaatgg cacgtttacg
     2101 agcaatctgg cgttcatcca ccttcatgtt ttgaagtgct ggattgttgg ccatacctgg
     2161 aatcatcttg agcaagtctt ccatcggccc catattttgc acctgatcta attgatcgat
     2221 gaaatcatta aaatcaaagg tgttttcgcg catcttctca gccatttcaa gggctttttg
     2281 ttcatcgtat tcctgagaag ctttctcaat caaagtgagc atatccccca tgccaaggat
     2341 acggctagac atacggtctg ggtggaaggt ttcgatatct gtaatttttt cacctgtacc
     2401 agtgaacttg attggttttc cagtgatgtg acgaaccgat agagccgcac caccacgagt
     2461 atcgccatca atcttggtaa ggatgacccc agtcacttcc aactgagcat taaactcacg
     2521 cgcaacattg gctgcttcct gaccaatcat ggcatcaacg acgaggagaa tttcatttgg
     2581 ttgagccaat gctttcacat cacgaagctc attcatgagg agctcatcaa tttgcaaacg
     2641 acccgcagta tcaatcaaga catagtcgtt atgattagtt tgggcttgct ccaaaccttg
     2701 acgtacaatc tcaacagctg gtacttctgt tccaagtgca aagacaggca catcaatctg
     2761 ttgtcccaaa gtcttaagct ggtcaatggc agctggacga taaatatccg ccgcaatcat
     2821 caaaggacga gcattttctt ctttcttgag tttgttggcc aatttaccag caaaggttgt
     2881 tttaccagcc ccttgtaaac caaccatcat gatgatggtt ggaatcttag gtgacttgat
     2941 aatttctgcc gtatcagaac ctaaaacggc tgtcagttcc tcatcaacga ttttaataat
     3001 ctgttgcgca ggattaagtg tatcaatgac ctcatgcccg actgcacgct cacgaacttt
     3061 cttgataaag tcctttacaa caggcaaagc aacgtcggcc tcaagcaagg ccaagcgaat
     3121 ttctttggtt gcctcttgga catcagattc agagattttt ccttttttac gtagactttt
     3181 aaagacgttc tgcaaacgtt ctgttaaact ttcaaatgcc atttttcttc ctcttattct
     3241 ctattatcaa tgcttgttaa aatttctatc tgctcctgca gaaagttatc cttgggatag
     3301 cgctccaaaa tctgatcaaa aatctgactg cggacaatat agtccgagta catgtgcaat
     3361 ttcatctcat aatcttccag aatcttttct gttcgcttga tattgtcata gacagcctga
     3421 cgactgacac cgaactcctc ggcaatttca gcaaggctgt aatcatcagc gtagtagagc
     3481 tcgatataat tcatttgctt atctgtcaaa agcgccgcat aaaattcaaa gagcgcattc
     3541 atacgattgg ttttttcgat ttccataact tttattatac caaaaattag cctaatctac
     3601 cacactagga agccgatcca agaagataga tagctaaatt tgaaaaagac ataagcctag
     3661 ccccaagtaa tttccaattg atagctggca aagggatgtc cctcttgatt ttgtagttga
     3721 taatctagtt caatcttttg cccatccact tgataacgat tcgtttggat aataaactcc
     3781 tgcataccca taggtgtggg aatataggct aaactatcac tatcctttag aaagcgcata
     3841 atggtcttgg gattggaaaa tcgactcatc acaagttctt gaccatgaaa tttaatcact
     3901 actttttcct tttcctcatt atagaaaagc aggtagctat aatctccttt ttcatgcact
     3961 tccacatcat aaagctggtc aatcacttcc aactgctcat caaactgaat tgtatttcgc
     4021 atccgaatct tcacatcagg ccctctttct tgtctcttgt cctactattt taccaaaaag
     4081 agcaggattt tgctataatg gtcatatgaa cgaaaaagta ttccgtgacc ctgttcacaa
     4141 ctacatccat gtcaataatc aaatcatcta tgacttgatt aatacaaaag aatttcagcg
     4201 tttgcgccgg atcaaacaac tgggaacttc cagttatacc ttccacggtg gagaacacag
     4261 tcgcttctct cactgtctag gagtctatga aattgcacga cgcatcacag agattttcga
     4321 agaaaaatat cctgaggaat ggaatcctgc cgagtctctc ttgaccatga ccgctgctct
     4381 cctacacgac cttgggcatg gtgcctactc ccatactttt gaaaatctct ttgatacaga
     4441 ccatgaagcc attactcagg agattattca aaatcctgag acagagattc accaagtcct
     4501 gctacaagtg gcacctgatt tcccagaaaa ggtggccagt gtcattgacc atacctatcc
     4561 taataagcag gtcgtgcagc tcatttctag tcagattgac gcagatcgca tggactatct
     4621 cttgcgcgac tcctatttta caggagcatc ctatggggaa tttgacctga ctcgaatcct
     4681 ccgagtcatt cgtcctatcg aaaatggtat cgcctttcag cgcaatggca tgcacgccat
     4741 cgaagactac gtcctcagtc gctaccagat gtacatgcag gtttatttcc accccgcaac
     4801 acgcgccatg gaagttctcc tacagaatct tctcaaacgc gccaaggaac tctatcctga
     4861 ggacaaggat ttctttgccc gaacttctcc acacctcctg cctttcttcg aaaaaaatgt
     4921 gaccttgact gactatctgg ctctggatga tggcgtgatg aatacctact tccagctttg
     4981 gatgaccagt cctgacaaga ttcttgcaga tttatcgcat cgctttgtca accgcaaggt
     5041 ctttaaatcc attacctttt cacaagaaga ccaagaccaa ctcgctagca tgagaaaatt
     5101 ggttgaggac atcggctttg atcccgacta ctatactgcc attcataaga actttgacct
     5161 cccttatgat atttaccgtc ccgaatctga aaatccaagg acacagattg agattttaca
     5221 aaaaaatgga gaactggcag aactctctag cctgtcccct atcgtccaat cccttgctgg
     5281 aagtcgccac ggagataatc gcttttattt cccgaaagaa atgttagacc aaaacagcat
     5341 cttcgcaagc atcacccagc aatttttaca cttgattgag aacgatcatt ttaccccaaa
     5401 taaaaactaa aagaggaaat ttatgagtat taaactaatc gccgtcgata ttgacggaac
     5461 cctagtcaac agccaaaagg aaatcactcc tgaagtcttt tctgccatcc aagatgccaa
     5521 agaagctggt gtcaaagtcg tgattgcaac tggccgcccc atcgcaggtg ttgccaaact
     5581 tctggacgac ttgcagttga gagaccaagg tgactatgtg gtgaccttca atggtgccct
     5641 tgtccaagaa actgctactg gccatgagat tatcagcgaa tccttgacct atgaggatta
     5701 tctggatatg gaattcctca gtcgcaagct cggtgtccac atgcatgcca ttaccaagga
     5761 cggtatctat actgcaaatc gcaatatcgg aacatacact gtacacgaat caaccctcgt
     5821 cagcatgcct atcttctacc gtacccctga agaaatggct ggcaaggaaa ttgtcaagtg
     5881 tatgttcatc gatgaaccag aaattctcga tgctgcgatt gaaaaaatac cagccgaatt
     5941 ttacgagcac tactctatca acaaatctgc tcctttctac ctcgaactcc ttaaaaagaa
     6001 tgtagacaag ggttcagcta ttactcactt ggctgaaaaa ctcggattga ccaaagatga
     6061 aaccatggca atcggtgacg aagaaaatga ccgtgccatg ctggaagtcg ttggaaaccc
     6121 cgttgtcatg gaaaatggaa atccagaaat caaaaaaatc gccaaataca tcactaaaac
     6181 aaatgacgaa tccggcgttg cccacgccat ccgaacatgg gtactgtaaa agtatcattt
     6241 ttcaataaga attgattagc aataaaatcc aatgaatttt tttagcaaac tatttaattt
     6301 aaaacaaaat aatcataata gagacacaaa ttctgattgt aacaattttt acctaaacga
     6361 tttagaatgt ggccttactc ctgggcaact catactcata gattggactc aaaaaacagg
     6421 gagaaattat aatttcccaa gatattttaa atactctctt caaattgacc ctgaatctac
     6481 acacaatcaa ttatacaaat taggatactt cactaaaaat aagactttat catatcttac
     6541 agtagtagaa ttaaaaacta tattatctaa acataattta gctacttctg gaaaaaaagc
     6601 agaattaatt acaagaataa ttaataatgt taacattgac aatttagata ttccgttcga
     6661 atttaaacta acaaaagaag cacaaaatct tattatcgaa catagtgact atatcaaagc
     6721 atactatgat aaagacataa ctatggaaga ttattgtaaa gaaaaaaaca atatctcttt
     6781 taaagcaact tttggtgata taaaatggag tctcttaaat aaacaagctc ataggaatac
     6841 tgtatcagga gattttggat gcttatctaa cacacgaaag gctcagggaa gacatttgga
     6901 acaagaaggt aatattaaac atgctttaat atattacata gaatctttga taattactat
     6961 ttcaggatta gaaaacaatt tttcagccac tgattatcca gtatattatc ccgattcgat
     7021 acctgactac tcactaaaac atattcaaac attaatggaa tcattatctg atgacgatta
     7081 tgattttgct tttgatgaag cattatttcg cttctcaatt ttgaatgcaa atcatttttt
     7141 atctaaggaa gatattgact atttaagagt taatttacct cgttccactg ctgaagaaat
     7201 aaacaattac ttaaagaaat atgaatgtta tagtccttta aataatttag aacttgacga
     7261 ttttgaataa attgactata caaacattta tatactcgat atagtctcaa ttttatctga
     7321 tgattgccca aatttttcaa taataaaacg cataatatta tggagacaat cccctatatt
     7381 atgcgttctt ttaatatcaa agactttttg acaaacttct tcgatatcta attactgccc
     7441 cctgcaggaa tcgaacctgc aactactcct taggagggag ttgttatatc cattgaacta
     7501 agggagctag ataaaaactc tgctgaaaag cagagttttt tagtcgaatt aacgacggat
     7561 ttctttgata cgagctgcct taccttgaag agcacgcaag tagtacaatt tcgcacgacg
     7621 tactttaccg taacgaacaa cttcgatttt ttcaacacgt ggagtgtgga ttgggaagat
     7681 acgctcaaca cctacaccgt tagagatttt acgaactgtg tagttttctg agattccagc
     7741 acctttacgt gcgataacaa caccttcaaa aatctggata cgttcacggt taccttcgac
     7801 aactttcgca tgtacacgaa cagtgtcacc aggacggaat gatgggatat ctgtacgaag
     7861 ttgaccttca gtcaagcttt ggattaatgg attcatttta ttctcctatc tttgtcaatc
     7921 ttgaggaatc ttcctcagcg gataaactgt atttttgtgc gtccattaca cacaagatat
     7981 agtttaccaa atttctaaaa aaaagtaaag aaatttatag caaaattcct aaaaaaatcg
     8041 caaccaaacc acctatgtaa gtcaaagtca ggtaagaata aaagaccttc ttatcactta
     8101 gcagtctttg aagttcgtca ttcaaggtcg aaaaagttgt taaaccgcca caaaatcctg
     8161 ttgctagaat agcatatact tccttagact ctacatgatt gtagaatact ccaattaaaa
     8221 aacaacctaa aagattagct atgagcgttc cgagtggcaa tttagaagct tgattatagc
     8281 gagaaaagaa ataccgtacg agtgctccca agccacaggc gattgcaaga tagacgatta
     8341 ccatttcttc ctccccaaat agtaagctaa aagcaggcct ccaccgatac tcaaaagcaa
     8401 atacagtatc aaactaagat aacgccctgt atcaagcagc ttcacagtat caagcattag
     8461 gctggaaaaa gttgttaaac ctccgcaaaa acccgtcccc agtgctaaaa tcaagccctt
     8521 actagtcccc ttatagacca gatagccctt gacaagataa atcaagcaga aaattcccag
     8581 atagttgaca aagagagtgc cccaagggaa atctggactg gctggtaacc aagtggaaac
     8641 taggtagcgg acaagtccgc ccaacatagc agctagaaaa atccctagcg gataaaattg
     8701 ttcttttttc atttgatatt ttgatcctga taatcacgcg aacgtttgag tatgtccgaa
     8761 aaagtcgcga caatagtctc ctgatagcgc ttgtcctcta cacgacttct aaccttttca
     8821 aaaataactg cttctctctt agtatctaaa atcggtttac ctgaagcttt cttataagca
     8881 accaccccct caactaaatg cattcgttct tctaacagtt tgacaatttg gtcgtcgatt
     8941 tgatcgattt cttgacgaat aatatctaaa tccatacagt ctcctccttt atttgaatta
     9001 ttgtatcaaa aagccatcaa ttaggctagt aaaatcccaa ctctcgataa gaaaaatgca
     9061 ctgattgatt acatcagcgc acttttatgt ttatcctact ttagcagcca attcttctgc
     9121 gaattgttcc aagcgttcaa tatcttcttc ctcggcagaa agatcaactt taacacactc
     9181 tgaacctttt tctgctcctg ttgacacaaa gacacggtca aagtcatcaa cagccttaca
     9241 gaattcatcg tagaaggtat ctcctgagcc gaccactccg tagattttgc cattcaagtt
     9301 gagatctgct aggtcttcgt agaagtccat catctcatct ggcaattcac catcaccata
     9361 agtataggtc gcaacgatag cgatgtctgc ttccaagaag tctgaagcgt caacagttgt
     9421 acattcatca acatcaacat ccaagcccaa gtcacgtaat ttgtctgcta caatatctgc
     9481 aatttcttcg gtattaccgg tcatactggc aaatacaatt tttgctaatg ccatatcgtc
     9541 ctcctcaatt tatctttctc cattatatca catctttttc attttaaaaa taaaaattct
     9601 tgtgctacaa tgaaatgaga aagaattgag gttatttatg gagatttgcc aacaaatttt
     9661 agaaaaaatt aaagaatacg acaccattat cattcatcgt catatgaaac cagaccctga
     9721 tgccttggga agtcaggtgg gattgaaagc cttgctggaa catcatttcc cagaaaaaac
     9781 catcaaagcc gtcggttttg atgaaccaac tcttacttgg atggctgaga tggatcttgt
     9841 tgaagataga gcctaccaag gcgcacttgt catcgtctgt gatacagcta atactgctcg
     9901 tatcgatgat aagcgctata gtcaaggtga ttttctcatt aagattgacc accatccaaa
     9961 tgatgatgta tacggtgacc tgtcttgggt cgatactagt tcaagtagcg ctagcgagat
    10021 gattacccta tttgcccaaa caacccaact agccttggca gatcgcgatg ctgagttgct
    10081 ctttgcagga attgtcggtg atacaggtcg cttcctctac ccttctacca ctgcacggac
    10141 tcttcgcctg gctgcttatt tgagagaaca taactttgac tttgcggctc tcactcgcaa
    10201 aatggacact atgagctaca aaattgctaa actgcaaggc tacatctacg accatctgga
    10261 agtggatgaa aatggtgctg ctcgcgttat cctgagtcaa gaagtcttga aacaatacaa
    10321 tgtaaccgat gctgaaactg cagccattgt cggtgcacct ggacgcattg acagagtgag
    10381 tctctgggga atttttgtcg aacaggctga tggccactac cgagttcgct tacgcagtaa
    10441 aatccatcct atcaatgaaa tcgccaagga gcatgatggt ggaggccacc ctctagcaag
    10501 tggtgctaat tcctatagcc tagaagaaaa cgaaatcatc taccaaaagt taaaaaactt
    10561 gcttaaaaac tgataaaata cttgccaaac ttttcagaat ctgatagact agtatggtaa
    10621 caatctatgg ctcgcaaaga gaccatggca gaaaggaaat attgcaaaat gaaaaaagat
    10681 atccatccag aatatcgccc agttgtcttc atggacacaa ctactggtta ccaattcctt
    10741 agcggttcaa caaaacgctc taacgaaaca gttgagttcg aaggcgaaac ttacccattg
    10801 atccgtgtgg aaatttcatc agactcacac ccattctaca ctggacgtca aaagttcact
    10861 caagcagatg gacgcgtgga tcgtttcaac aaaaaatacg gtctcaaata atgataagag
    10921 aacagtttcg gctgttcttt tttgtttctt gaaatcaact gctgttttca tgttccagac
    10981 tcatctgtag gttcggtttc catgctacta ggcaggaagg aaatagctgt ttcaacacgt
    11041 ccataatgag ctgtactatt gtcacgaacc acactttcat tgatggtcca agtggaattc
    11101 attttcttaa aagcttctcg gactttttcc aaatctttgg aggcaatggc ctgttctaag
    11161 gtttcaaaac gaggacttag acggtttacc aatttttcct tttccgcatc taggtcaaca
    11221 ggattttgtt ctttttcaaa agctaataga gctgaggaaa tttcggtgag tttggcttca
    11281 gtaatatctc cagatagagc taacttttcc ttaacaatct taccaacctc agaatcctta
    11341 ttttctactc tttcgaagtc tgatgccatc ttcgtaacga gtttctgggt ctcagtctga
    11401 ccaccatttt taaccgtttt ggatgcatct gtgattttca caaagaaaaa actcaagctt
    11461 tcttgggcac ctgctgggga aagaattaaa aatgacaaag ctaaaactag taaccagctt
    11521 ttagccttcc aataatttct gaccaatgta gcctcctttc tccacaccac caaagcaagt
    11581 aaaaagtcca ctacctatgt gagtgatgta ttcattcatc ttatcagtag cacctagatt
    11641 agtttgaacc ttaacaaagt tatcaggatc cttctggaaa gaaatgaaga gcaatcccgt
    11701 atcaaactga ccagtctttt catcaatgcc atctgagtaa gaataagaac gacgtaaaag
    11761 tggcttatcc acttctttgg ccaaacaaac atgcgaatca tcaggtaaga gactcaaatc
    11821 cacttcatca aactcatttt tcttaccaaa gggggcaccg ctttccttgt aacgaccaaa
    11881 ggtattttct tgttcttcga gactagtgcg gtcccaggtc tctaaaaaca tctgaatccg
    11941 acgaactgcc atataagaac cattttccat ccagtcctta ctatcagccc agataacgcg
    12001 gtcaaagtct tgctccttgg ttggatttgc tgttccatcc ttaaaaccaa agaggttcca
    12061 cggagtctcc attcgatccc cgatagctgc aaatccagac tgactccaac gaaggggcac
    12121 cgcatttctc cccttacgga tgagattgcg aatagcgtga aaggcaattt gctcatcatc
    12181 tgcacaagca tgaatcacaa tatctccacc agtatatttt tcacgtaatt gctccttagg
    12241 aaatagaggt aaatccctga acagtcgagg gcgcttgttt tccaagttca tccttttcaa
    12301 gaaacttgca gatacaccaa aagtcagcgt caaacgatga ggattgagcc caactgtctc
    12361 accagtatcg ctcggaggaa agagagcatt ctgtccatct tttttaacca attccccctc
    12421 gaccaacttg gcactataat ccgtccaatc cttgaatagc tggataattt tatctctatc
    12481 cgtcgtatgc aggtccaaga caacaaaata gatattcttt tgcatgggcg tactatttgg
    12541 tacatcaagc gaaccttacg aaaacgtcta ctatatttaa accgctttct tgtcttcttg
    12601 aattgagatt ttatttttag acgcgacaat ggtttgagcg tctgctttct taggatgttc
    12661 tggacgtggc ataatatttc gccaaccatg gcgcttcaac agttgataga aggcgtcacg
    12721 tgtgtaggaa cgacctaact cctttttata agcctgaaat aaggcatcaa ttgtaacaaa
    12781 ttctcctgac tctgcagcct tcaaatgacg ggcaagaaag gctttctctt cctcaactgt
    12841 catatatgca tggttacgac cactacgtgt ttcttgaagg agagagtcga gtccgaactc
    12901 ctcatatttt tttacatttc gctaaatcgt tgtttgatta cagtctaaaa gttctataat
    12961 ctctttataa gatttgccca tcagacgaaa tataataaat tgaaactaga atagtacacc
    13021 tctacttcta aaatatt
//
LOCUS       DBEJAQ010000048        12908 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.048, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000048 DBEJAQ010000000
VERSION     DBEJAQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.048"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(98..610)
                     /locus_tag="ACL297_001668"
     CDS             complement(98..610)
                     /locus_tag="ACL297_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin C-terminal
                     domain-containing protein"
                     /protein_id="HGR0071898.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(635..1210)
                     /locus_tag="ACL297_001669"
     CDS             complement(635..1210)
                     /locus_tag="ACL297_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HGR0071899.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            complement(1220..1615)
                     /locus_tag="ACL297_001670"
     CDS             complement(1220..1615)
                     /locus_tag="ACL297_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HGR0071900.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            complement(2091..2900)
                     /locus_tag="ACL297_001671"
     CDS             complement(2091..2900)
                     /locus_tag="ACL297_001671"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGR0071901.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(2897..3457)
                     /locus_tag="ACL297_001672"
                     /pseudo
     CDS             complement(2897..3457)
                     /locus_tag="ACL297_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            complement(3459..4670)
                     /locus_tag="ACL297_001673"
     CDS             complement(3459..4670)
                     /locus_tag="ACL297_001673"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGR0071902.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(4684..5367)
                     /gene="rpiA"
                     /locus_tag="ACL297_001674"
     CDS             complement(4684..5367)
                     /gene="rpiA"
                     /locus_tag="ACL297_001674"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGR0071903.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(5665..6504)
                     /locus_tag="ACL297_001675"
     CDS             complement(5665..6504)
                     /locus_tag="ACL297_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HGR0071904.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(6518..7339)
                     /locus_tag="ACL297_001676"
     CDS             complement(6518..7339)
                     /locus_tag="ACL297_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HGR0071905.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(7476..8333)
                     /locus_tag="ACL297_001677"
     CDS             complement(7476..8333)
                     /locus_tag="ACL297_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HGR0071906.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(8487..9221)
                     /locus_tag="ACL297_001678"
     CDS             complement(8487..9221)
                     /locus_tag="ACL297_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGR0071907.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(9221..9904)
                     /locus_tag="ACL297_001679"
     CDS             complement(9221..9904)
                     /locus_tag="ACL297_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGR0071908.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            10094..10324
                     /locus_tag="ACL297_001680"
     CDS             10094..10324
                     /locus_tag="ACL297_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HGR0071909.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            10553..12811
                     /locus_tag="ACL297_001681"
     CDS             10553..12811
                     /locus_tag="ACL297_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGR0071910.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         3865 a         2915 c         2318 g         3810 t
ORIGIN      
        1 aattttgttc tttcacagag agctacctga gttctattca agctcagata gtactttctt
       61 ataaactaga caaactaact gtcattgtac catcagatta caagacatca tcgtcactca
      121 ccttggaatt caatgtcgta ccccaatggg taattttacg gtggggttga gctaaaattg
      181 gtctgttttc atagattgtt tgccatctat tccatagtag gcccgtcttt ttctcaatct
      241 taactcgcag atttctcata ttttctttga ttgggaagtt gaggacaaaa cctgcagtct
      301 ggttgcaacc gtttccttcc caagaatgac tacgaacaac ttggtttcca tctttatcta
      361 ctggaacttc ttcccaagtt atggagtagc gggcaatgta agctccactg tgttgaattg
      421 tcaatgtttt atctttcaca ggagtctgac tgattggttg aactggctta gaaacttgtg
      481 tcgccgtttc agcattcgta gctataaagc ggaaaacttc aacttctgca agactgaggg
      541 cattatagcc ttctagccca atccgaacat agcgcgcttt tttatggtta atcgtaattt
      601 gtgctgacac atctttagga gatattatac gttttcactc aatttcttta ccagaactgt
      661 ctaaaagaat gacatcaaag tttgccaatc tatcctgggc agtgtctgtt cggttgtaaa
      721 tattgatttg gcgaatggtt tcttctttag ccaaatctac ttgccaccaa ggcttagatt
      781 ggaagtttgt atgagtgaca gaattgtgac catagttacc atcgactttg ccatccacag
      841 ctcttctagc atctcctccg aaagctgtcg aactttgagt aacctgtttt cctaaggcaa
      901 tattttccat tggttcagcg cttggttgac tagaagattg aggtagagcc tgtttcttaa
      961 agaatcgcac tttatccaat ttcggatcac gataaccatg tttatccaag gtagcttgtt
     1021 taccaatatt aaagttagga atatcattca ttggactttc aaaatagcca cgagaacggt
     1081 gtttaaagcg tgaattacct gtaaatgtaa caatatcacg attattatga atcaagggag
     1141 tcacaacatt gtcattatca accacataat gcttaatttt tccactaaca gctaatttac
     1201 gactttccaa accaacatct cagaaaccat tcttagcatc ccaaacagtt ctggaagtaa
     1261 tcactttagg agcactgaaa gttgtcactt tcctcaatac atggttataa aaatcaggat
     1321 atttttggta atcttcaacc gctgcaatct gagctaggta gcctccaaga gaatgacctg
     1381 tcatatacaa cttagtaata cttatatcct tggctaattc cccaacaacc ttgcggatgt
     1441 catccgcttg agctggctct gttatctcta cttttgactc ttcccttttg ttttcaagaa
     1501 cttcacttgc ttccggaagc gtttgaggag ctagtcttgg ctcctctcta agaggagttt
     1561 ctagttttgg agcatcattt tctgactctt tcaattcctc cttagaatct agcacaccta
     1621 ttaccacttc agttgttaca tcttgcttta cttcattggc tgaaattcca gctggagcta
     1681 gaaatcaaag ccgactacaa cagatacaac tccgatactt aattttttat gccaaagcgc
     1741 ataattcgtt gaccaatttt cattatttct tatctttctt ttcgtttatt tacaagcaga
     1801 ctcaagcgaa atctcattgt gctatatatg ataagactga gaaattcaga aagtcaattc
     1861 attctcatct ctccttatca tactagtctg cttggctagg aagctaatgc tagtatccta
     1921 gaatcttgtc tttcacaatg ctggctatca gatcagacac acctattctt ccgcttcttt
     1981 cagttataag agcaggaaga agctcatagt attcctctgt ttcttcttta atacggatag
     2041 gcagtcaatc ctgaaaagac agaggcactc ccccttttaa atcttttttc ttacaattca
     2101 gcaagaatcg ctttaagcaa gcctttaaaa tcacctttaa cacgttcagt cacttctaca
     2161 acttcttcgt gattgagttc ttcttggaaa ccagccgcaa agttagtgat acatgaaatt
     2221 cccagaactt tcaagccaga gtgggctgcc acgataactt caggaaccgt agacatacca
     2281 actgcatctg ctcccagtgt cttataggaa cgaatttctg ctggtgtttc ataagtcgga
     2341 ccagtaactc cgatatagac accttcatca agcttgatat taagtttttt agccacttca
     2401 tgggcagtag cacggtattc tggtgtgtag gccctagaca tatctgggaa acgtgggcca
     2461 aagtcatcca agttttcacc catcaatgga ttttgccccg tcatgttgat atggtctgag
     2521 atagccatca aggtaccagg accaaatccg ataccgccag ctgcattggt tacaataaca
     2581 ccttcacatc caagaacttt catcacacga actgggaaag tcaccacttc cagaggattc
     2641 ccttcataga aatggaaacg cccttgaaga gccaagacct tgcgacctgc cagttcacca
     2701 tataccaatt taccagcatg accgactact gttgaacggc cccagtttgg aatctcagca
     2761 tagtctacta caactggatt ttcgatttct tctgccaatt ctccaagtcc tgacccaagg
     2821 attagaccga actcaggggc tgcaattccc ttttctttca ggaaagtagc agtttcatgg
     2881 attttgttta aaaatgtcat tgtgtgtctc ctttattatt tttttagcat ttgaccaatt
     2941 ttgtcaaagg ttttagcatt acgaatagta atgtggcggt agaaaggcac ctcagcaagt
     3001 acttatgata ggcagtctta gaataggatt cttcagaaaa tttcccctag aaaatcccaa
     3061 gttttccaaa ataaagcact tcatctttca gctctaaact ttcaactgtc gcgatgactt
     3121 ggtccacatc caaaccctca gtgtaaaaga gaaaatcttt tcgtgccaaa tctctgctcc
     3181 accaagctgg tagattttca agttccgcct caaagtcctc tagactcagt aaagaaaagc
     3241 tctgaataaa tggataatgg actgcaaaga aagtctctag cttttcaacc aattgggctt
     3301 tggaatctat cgaagtaaag aaaatattgc cactgttgat gtagctctca accttttcca
     3361 gtcccaagtt tgtcaattct tgacgaagct ccgccatgac gaccttattc ttaccaccaa
     3421 cattgatacc tctcaccagc aaagcatagc gcgtcatctt ataccaattt atctaagaaa
     3481 ctttccccaa tcatagcagt ttccacacca aagttatcgg caacagtcgc tgaaatatct
     3541 gcaaaatgtc ctactggaat gagaccattt cctttaaagg cagggctata ggccaacaat
     3601 ggaatatatt cccgagtgtg atccgttcct gcatacgttg ggtcatttcc atggtccgca
     3661 gtaatcaaga gaaggtcatt ctctctcata gctgcgataa tttcaggtaa gcgttcatca
     3721 aactcatgca agcaatcacg gtaaccgtga gcattacgac gatggccgta aagggcatca
     3781 aagtcaacta ggtttgtgaa tgagaatcct ttttcaaact cagcaagtcc catagtcttc
     3841 aatagtgtat caattccatg actatttgac ttgttgtgac ccatgtcatg gttgatacca
     3901 gcaccgttaa agatatcgtt gattttaccc acagcataag tatcgatacc agcctcattc
     3961 aatttatcca aaacagttgg ggaaaatgga gatacagcca agtcacgacg gtttgccgta
     4021 cgagtgaagt tacctggttc acctacataa gggcgagcaa tgatgcgacc aagaagggca
     4081 ggacgctcaa gggtaatcga acgagcgtat tcacagatac ggtacaattc atccaaagga
     4141 ataatgtctt cgtgggcagc aatctgcaaa acagggtcag ctgaagtata gataatcaac
     4201 tctccagttt ccatctgacg tggtccaaaa tcatcgataa cagccgttcc tgaataaggt
     4261 ttgttggctt cacgaataac cttgcgtcct gagaattctt cgatttttgt caggatttct
     4321 tctgggaatc cgttccagaa agtatcgaaa ggctcagtaa tgttgagtcc catgatttcc
     4381 cagtgtccag tcatagtatc cttaccaaga gatacttcct ctaattttgt tgcatatcca
     4441 gttggattgc tttcagctgc tacagtctta agaggagttt cacgaggaat atttccaaga
     4501 cctattttag ccatgtttgg gacattcaaa ccaactgttt ttgaaatgtg tcccagtgtg
     4561 tcagaagctc catctggaac ccctgcattg acaaagttat tagcatctgg tgctgcaccg
     4621 attcctacag aatccagtac caccaaatga atacgattaa atttagacat cttatgcccc
     4681 cttctatttt cctttttttg aagttgaaat ctgaactcca tcccgtccag caacgatgac
     4741 cttatccacc atttggttga ataaaccatg ctccacaaca ccaacgacat ggtccaattc
     4801 ttgtccaaaa gcaattggat tttcaatgac atccaaggcg aggtcaatga taaaattctg
     4861 catatccgtc acaaaacgtt ggccgtcttt ttcacggaaa cttggtttgt agccagctcg
     4921 ttcaaaacga cgaaagacct gttctgcacc atactgaacc acttctactg gcaatttaaa
     4981 agcacctagt ttttcgacca gcttgctttc atccaccacc caaatgtatt cttttgatgg
     5041 tgtcgcaaca accttctcca tcagaagagc accaccacca cctttgattc cattaaactg
     5101 actatccact tcatctgcgc catcaactgt cacatcgaca aagtctactt ggtcaataga
     5161 tttgagtggg atattaaggc cttctgcctg tttagtggtc acactagaag tcgttacagc
     5221 tataatctgc aatccttctt ccttgatacg acgaccgatt tcttcgacaa aataataggc
     5281 agtcgagcct gttccaagtc caacgaccat tccatccttg acaaactcag cagccttgat
     5341 acctgccatt ttcttcagat tttccactca aacacctcca ttaaagagct acctctatta
     5401 taccatgtaa aggcttttat ttcatctaaa agtaccttta gaatactgct tatttatcct
     5461 tgaaattaaa tttttttaat aaaagcagaa ctctgacaaa tcactttaag tacggatgta
     5521 aaccaaatca actctaacaa aaatagattt acaagagtaa aaaaacgaac aaatttagtt
     5581 tcttagcgag aagaatacta aacgttcgtt ttttgtgaat taagaataat gatacaaatt
     5641 atcaatattt tttgtctaac tattttagac atatcttttc attactttag gaagacaatt
     5701 actaatctcc gttggcaaaa ccacatactg ttcttgagct agttcttggg ctatggctga
     5761 atgaagatgg gtcgctactg ccacacgttc gtaaagacta gcttgcttaa actggcctgc
     5821 aaatcctgca atcatcccag caagcgtatc tcccattccc ccagtcgcct gatagggacc
     5881 gccaacctgt aactggtaat aatctgattg gccagcttgc caaatacgag tagctgaacc
     5941 tttctccacc aaaattgttc cttgagggaa ggaagttagg gcactagccg ttgcatcttc
     6001 cttttgcttt tcaatcgtaa ttccagataa tttttcccat tctttttggt gaggagttag
     6061 gataagctgg cttgaggaaa atgacaaact tgtcctagca agaatggtca aggcacctcc
     6121 gtctacaatc aaaatctgat tttgtcttaa gccagcaaag acctgtttaa ctagatcttc
     6181 tccaaaagca tcgtctcgta aacccggccc cagcaagaca acttctgcct tctccaattg
     6241 ctcttttaac aactgctggt cttgaagaga aaaggccata gcctcaggta aatggctgtg
     6301 cagggctgga atattttccc tatctgttcc aacggtcacc aatcctgcac cgctttttac
     6361 agctgccaaa gcagccatga tgatggcacc accataagga taagttccac caagaaacag
     6421 cagacggcca tagtctcctt tatgactagt acgagaacgt tcaataataa ctttttctag
     6481 taaggcttga tcaatcactt tcatcgttct tcctcaacta tcctttatct gattgtttta
     6541 aaacaacttg atagactttc ttagtccact cttctaagtc ttctccagca tagtcaagat
     6601 aatgcaccct atcttgtatt ttagttggaa taatattttc aaattggctc ttattatgag
     6661 ttggaataat acaaatatac aactcactaa ataaattcaa catgtcctta atttttgcaa
     6721 catggtagcc atcaaaaata taaccagtaa ctttaatcaa atctgtatag ataaaatgaa
     6781 aactcactcc aggaataaac ttgtcttgta attctttctc tgaaggagct cgacctaata
     6841 aacgctccat cgctaatcga taaccagaac ttgtatttga ccaccctaac atgacataat
     6901 caaaataatc tatcggatcc gatgcagcat ttctactatc caaaaccaac tcttcagcat
     6961 ctcgcctaaa ggctttcaca gcagataaga gctgacctgt tttaaagata gaaatcgctg
     7021 catctatagt cgtagtatga ctacagtagt ctgaagtcac taacctctta tgtagatcat
     7081 aagtcgaggc tactctttta tctgtagggg taaatggtgg cagaaaggga tgctgacaga
     7141 tgtaatctaa aatttctttt tttaaacaag aatcagcaca ctcaatactt gtttgacgtt
     7201 tatgaagttt ttcgtaagct ataaatttag caattttttc taattcccac tcttgaatat
     7261 ttggataatc aattagtgct aggtagtata ttccatccca ttgtaaacta tattccctat
     7321 tataaatttt taaaatcatc taatcacctc actccattat acaacaaaaa ggaggcgcag
     7381 acctcctttt gtaatcttat atctaaaatt taatattcat ttctgccatt ttaaatatag
     7441 ctatagaaaa tacactctct tcagcgattt ttctcttatt ttctatccaa tgtccgaagt
     7501 gctgcttgat aagtttgctc catcagcata gtaatggtca tagggccgac acctccaggg
     7561 actggcgtaa tatagctagc aagtggggca actgcctcat aatccacatc cccacagagc
     7621 ttaccatttt catcacgatt catcccaacg tcaatgacta ccgcacctgg tttgacaaag
     7681 tcagcagtca caaacttggc acgaccgatt gcgaccacca gaatatctgc tttagcagcc
     7741 accttagcaa gattatgggt gcgtgagtgg gtcaaagtca ctgttgcatt cttggccaaa
     7801 agaagctgag ccatgggttt tccaacgata ttagaacgac cgatgacaac cgcattttta
     7861 ccttctaatt caatcccata ttcatgaaac atttccataa ttcctgcagg tgttgatgga
     7921 atcatgactg gatgtccaga ccaaaggcgt cccatattta gaggatggaa accatccaca
     7981 tccttttctg ggtcaatagc caatagaacc gcctcttcat caatgtgttt tggtaatggc
     8041 aactgaacca aaatcccatg ccaagctgga tcctgattgt atttagcaat caggtctaac
     8101 aattcctctt gagtaatggt ctctggaact cgcactactt cgctacggga accagccgca
     8161 agagctgacc tctccttgtt gcgaacgtag acttggctgg ctggattatc cccaaccaaa
     8221 atcactacca aaccaggcac tagagctgtt tcttccttta attttgcagt cttttcagcc
     8281 aactgcccct gcaatttggc cgctaaagct ttcccatcaa taatctgtgt catatcactt
     8341 ctcttttctt tcaaatatct tccattatac caaaaactgc tagtgccctc taacacttct
     8401 ttcctaggtt atcctatagt ttcaaactat aagaaaaagc tctggttgaa cttttcacta
     8461 atcttgtctt gaaattttaa aatagattat aaaaccttac tcaagaaatc tttagtccgt
     8521 tgttcttggg tttgttcaaa aatctgctca ggtgtcccat cttcaacaac cacaccgtct
     8581 gccataaaga taacacgatc tgccacctca cgggcaaatc ccatctcatg tgttacgata
     8641 accatagtca tccctgactt ggcaaggtct tgcataacag ccaatacctc acctaccatt
     8701 tcaggatcca aggctgaagt tggttcgtca aagagcaaaa catctggttc catagccaac
     8761 ccacgcgcga tggcaatccg ttgttgctgc ccacccgaca gactctgtgg ataagcgtct
     8821 gccttatctg gcaaaccaac tttttccaaa agctcttggg ctctcttctc agcaacttcc
     8881 ctgctttcac ctttagtctt gataggtgac aaggtaatat tttccatcac agtcatatta
     8941 ggaaagaggt taaattgttg gaaaaccatg cccatcttct cacgcatggc aaacaggtca
     9001 ttcttcttgt ccgtaatatc gactccctca aagataagct tccccttggt tgcttcctcc
     9061 aacaaattca tagagcgaag caaggtagat ttcccactcc ctgaaggacc gatgataacg
     9121 acaacttctc ctcttttaat ctcgaggttg atgcccttca atacttcatt ctttccaaaa
     9181 gatttatgta aattttcaat ttttatcaag gtttctgtca ttatttctta tctccttgtc
     9241 ccatatgttt ttcaaaagct ttcaaggcta ctgtcagaat agaggtcata atcaagtagt
     9301 aaaatgctgc aaataaaagt ggtgttaaag gtagataggt tgttgtagaa actgttgtag
     9361 ccccattcca caactccatg accccaatag ctgataagag ggagctgtcc ttgataatgg
     9421 tgataaattc gttccccaat gctggcaaga tatttttgac tgcttgtggc aaaatcacat
     9481 aacgcatcgc atttttaaga cgaatcccta gcgaataagc cgcctctagc tgaccttttg
     9541 gaaccgcatt gattccggca cgaacagtct ccgaaacata agcaccacta ttcatagaga
     9601 taatcaaaat ccctggaatc agacgcgaaa aatcaacacc taaaattcca atctgaatag
     9661 tcggagcatt gatatgcata agagcaaagg caatcataat ttgaaccatc atcggtgtcc
     9721 cacggaaaat ccaaacgtac aagttggcca accaaacaag cggtttaaac tttgaacgtt
     9781 gcccaaaagc caagacaaca cccaaaatag ttcccaaaaa gataacacag atagaaataa
     9841 gaatcgtcac aacagcccca tagttaaaat aaggtaaata cttaggtaaa aaagaaaaat
     9901 tcatagtcac actgctttct aaaatagaat actgtatgat tataacatgt tttgaattaa
     9961 aatttaaatt ttttatccaa tttatttaaa aatctttaaa ctagcagaat tagcgagaaa
    10021 tcttagtttt ttctagtcaa gttgccctcc tttatggtaa aatagtaacg ataagaaatg
    10081 aaggagaatc aaaatggaat cacatttggt tagaatcatc aaccgccttg aagcaatggc
    10141 aaacgatggc ggaaatttaa aacgtaattt tgaacgcgaa ggagttgttg ttgcagaagt
    10201 tgcgtacagc cacgacgaag aaaacggccc aatctttacc cttcgtgacg tagaagatcg
    10261 cgaaacgtat acgtttgata gcattgactt aatcgcaatg gaaatttacg aacttctcta
    10321 ctaatctaaa acaagttggg attgtccctg catgtctaag taaaatcctt tttgtctcac
    10381 aaataggatt tttttatagt ccaaattcac aaaaattttc attgtaacaa cttctcaaag
    10441 cttcaatctt tttacttgct ttacacccca atcctgttat aatgagagta tagaatttga
    10501 ctatttttga cctttaaaca agtcaggcta aagaaagaaa tgaggtagat gtatgctttg
    10561 tcaaaactgt aaaattaacg actcaacaat tcatctttac accaatctca atggaaaaca
    10621 aaaacaaatt gacctctgtc aaaactgcta taagattatc aaaacagatc ctaacaatag
    10681 cctcttcaaa ggtatgacgg atctgaacaa tcgtgacttc gatccctttg gtgatttctt
    10741 caatgatcta aacaatttca gaccttctag caatactcct cctattcccc caacccaatc
    10801 aggtggaggt tacggtggaa acggcggtta tggttcccaa aatcgtggat ctgctcaaac
    10861 tccgccacct agccaagaaa aaggcctgct ggaagaattt ggtattaatg taactgaaat
    10921 tgcccgtcgt ggagacattg accccgttat tgggcgcgac gatgagatta tccgtgtcat
    10981 cgagattctc aatcgtagaa ccaagaataa tcctgtcctt atcggtgaac ctggtgtcgg
    11041 aaaaacggcc gttgtcgaag gtctagctca gaaaattgtc gatggcgatg tgccacataa
    11101 actccaaggt aaacaagtca tccgtctgga tgtggttagc ttagttcaag gaacggggat
    11161 tcgaggacaa tttgaagaac gcatgcaaaa actcatggaa gaaattcgca aacgtgaaga
    11221 catcatcctc tttatcgatg aaatccatga aattgttggt gctggttctg cgagtgatgg
    11281 taatatggac gcaggaaata tcctcaagcc agcccttgct cgtggagaac tgcaactagt
    11341 cggtgctact accctcaatg aataccgtat cattgaaaag gatgctgccc tcgagcgtcg
    11401 tatgcagcct gttaaagtcg atgaaccaac ggtggatgaa acaatcacta ttctcaaagg
    11461 gattcaaaag aaatacgaag attaccacca cgttcaatat accgatgctg cgattgaagc
    11521 agctgcaact ctttccaatc gctacatcca agatcgcttc ttgcctgaca aggccattga
    11581 cctcctagat gaagctggtt ctaagatgaa cttgaccttg aattttgtgg atcctaaagt
    11641 aattgatcag cgcttgattg aggctgaaaa tctcaagtct caagctacac gagaagaaga
    11701 ttttgagaag gcggcctact tccgcgacca gattgccaag tataaggaaa tgcaaaagaa
    11761 aaagatcaca gaccaggata ctcctatcat cagcgagaaa actattgagc acattatcga
    11821 gcagaaaacc aatatccctg ttggtgattt gaaagagaaa gaacaatctc aactcatcca
    11881 tctagccgaa gatctcaagt ctcatgttat tggccaagat gatgcagtcg ataagattgc
    11941 caaggctatt cgccgtaatc gtgtcggact tggtacccct aaccgcccaa tcggaagctt
    12001 cctcttcgtt ggaccaactg gtgtcggtaa gacagaactt tccaaacaac tggctatcga
    12061 actttttggt tctgctgata gtatgattcg ctttgatatg agtgaataca tggaaaaaca
    12121 tagtgtggct aagttggtcg gcgctcctcc aggttatgtt ggctatgatg aggctggtca
    12181 attaactgaa aaagttcgcc acaatccgta ttctctcatc cttctcgatg aagtggaaaa
    12241 agctcaccca gatgttatgc acatgtttct tcaagtcttg gacgatggtc gtttgacaga
    12301 cgggcaagga cgcaccgtta gcttcaagga tgccatcatt atcatgacct caaatgcagg
    12361 tacaggaaag accgaagcta gcgttggatt tggtgctgct agagaaggac gtaccaattc
    12421 tgtcctcggt gaactcggta acttctttag cccagagttt atgaaccgtt ttgatggcat
    12481 tatcgaattt aaggctctca gcaaggataa cctccttcag attgtcgagc tcatgctagc
    12541 agatgttaac aagcgcctct ctagcaacaa cattcgtttg gatgtaactg acaaggtcaa
    12601 ggaaaagttg gttgacctag gttatgatcc aaaaatggga gcacgcccac ttcgtcggac
    12661 tattcaagac tatattgagg acacaatcac tgactactac cttgaaaatc caagcgaaaa
    12721 agatctcaaa gcagttatga ctagcaaggg aaacattcag attaaatctg ccaaaaaagc
    12781 tgaagttaaa agttctgaaa aagaaaaata aatcctatag aaaaggagta gaaaatgaaa
    12841 tttttctgct tcttttttta ctaaaataac tgtaatttct tgacagcttg ccctttgtcc
    12901 attatgat
//
LOCUS       DBEJAQ010000049        12821 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.049, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000049 DBEJAQ010000000
VERSION     DBEJAQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12821
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.049"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(448..2175)
                     /gene="ezrA"
                     /locus_tag="ACL297_001682"
     CDS             complement(448..2175)
                     /gene="ezrA"
                     /locus_tag="ACL297_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HGR0071911.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2257..4203)
                     /gene="gyrB"
                     /locus_tag="ACL297_001683"
     CDS             complement(2257..4203)
                     /gene="gyrB"
                     /locus_tag="ACL297_001683"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGR0071912.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4218..4781)
                     /locus_tag="ACL297_001684"
     CDS             complement(4218..4781)
                     /locus_tag="ACL297_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGR0071913.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4866..5420)
                     /locus_tag="ACL297_001685"
     CDS             complement(4866..5420)
                     /locus_tag="ACL297_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HGR0071914.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5431..6654)
                     /locus_tag="ACL297_001686"
     CDS             complement(5431..6654)
                     /locus_tag="ACL297_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGR0071915.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6691..9141)
                     /locus_tag="ACL297_001687"
     CDS             complement(6691..9141)
                     /locus_tag="ACL297_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HGR0071916.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMVIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9341..10270)
                     /locus_tag="ACL297_001688"
     CDS             complement(9341..10270)
                     /locus_tag="ACL297_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGR0071917.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVVVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10410..10697
                     /locus_tag="ACL297_001689"
     CDS             10410..10697
                     /locus_tag="ACL297_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HGR0071918.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10731..12065)
                     /gene="ciaH"
                     /locus_tag="ACL297_001690"
     CDS             complement(10731..12065)
                     /gene="ciaH"
                     /locus_tag="ACL297_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HGR0071919.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12055..12729)
                     /gene="ciaR"
                     /locus_tag="ACL297_001691"
     CDS             complement(12055..12729)
                     /gene="ciaR"
                     /locus_tag="ACL297_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HGR0071920.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3898 a         2738 c         2282 g         3903 t
ORIGIN      
        1 aaaactctcg taaacaaaga ggttttagag gcctatttac cgtggactaa agttgtacaa
       61 gaaaagtgca aataagaaat ctccagatta ggaactatcc gtgagttctc tagtctggag
      121 atttttcaat agacttcgtt attggacggt taccaagtta ctcaaagtac agatttgaag
      181 ctgcagataa gactgacgaa atcactttaa tactaaatga aaatcaaaaa gcaaactaag
      241 aagctagcct caggctgtac ttgagtacgg taaggcgacg ctgacgtgat ttgaagagat
      301 tttcgaagag tataaaacac tgttttgagg tggtggataa atcggctggg taaaaactca
      361 atctcaggag aaaatgaagt aaatctcccc acaactatag aaaaagattt gattctgctc
      421 ctcacacaat aaaatctttt tcttttatta aaaacgaatc gtttcacgtg ttttctcata
      481 tgaggtaaca aagcgattgg ttacaccagg ctctgccact tccaatgctt gagaaatctt
      541 gtcaaatgaa gcgtgataat caaattcttt ttcaaaaata tctaaagctt cgttaaatgc
      601 ttcttgaatg cgttcatcaa atgagcgata gcggttagaa tattgcaaga gttgctctgt
      661 caaagttgca tattgtacaa tattataagt ttccgtttct aaagcttcca tatcattcgt
      721 tgcaatttca agaactcggg taacagattc aatgttaatc attttttgtt ctaactcaac
      781 cattaaatcc tcggtattat tgcttgccgt aaagaataac ttcaagaaag tttgtggaat
      841 acctggcaga ttgcgttttt ccatgtatcg cttgatagta tggagacgat tgacataaac
      901 attggccttt tgacgtgcat taatatcatc tttctcaatt tgtgtcaggc gctcactaac
      961 tgaaatttgc tcatcttcaa tatcttttag ttgagtttgt aaatcctcaa gattttcttc
     1021 aagaactgaa taagcttggg ttggttcttc ttgatttgaa gttacctcaa caatagctgc
     1081 ctcaaaactc tctaattctg tctgaatacg acgaacatgg cttgcagctg tctcaggaag
     1141 taaataggtc ttgttcaaac gtgcaatatc ttctcccaat aaagtattat tctctttcat
     1201 atgttgaagg taagttggaa gagttgcaag tagattttcc actactttct gagcagcaat
     1261 ttctcgagta aaaatatcat acaaggcatt gatttcctct tgtgcctgtc cattctcata
     1321 ttcggcatta tccaattcca actgacgaat attctcttgg tttttcttga atgcttcata
     1381 aagcaagtgg aaacgcgctt caatatccgt ttcaacaaaa tgataattag catcaattag
     1441 tttacgataa ccggattcca aatcctgtaa ttgatctggc aattctgtag aaagcgtcgt
     1501 aaccaaggct ggaacacgat ccacaatatg acttaaggcc aaaatgtgat tttctgtatt
     1561 atccaaaatc actgcggctt ccacagggtc acccgatgaa ttcaaggtta caaattgtga
     1621 aaattcagat tggatatttt ctaattgttt ttcaatttca tccaaggctt gaccatactg
     1681 ttctgaattt tcagcaactc tatgctgaag ttcctcaaat aaatccaaag catgaagaac
     1741 acgaccacta tttttagatt cttgcttctc taagtctgcc aaagcattgc gaattgccgc
     1801 aatatcttcg tcaatcaaag taatttgact ctcaatttgg tcaatttgat gactggcctt
     1861 gagaaaacga aatgaatggt tatagccttc tgcttcaaag agattatttt caatatcggc
     1921 aaaagagttg agagataaat cgacccattt ttgattccat tcacggaaag ccacttgact
     1981 ttgtccaatc aagtgcatat tttttacagc ttctacttca tcatttactg gaagattgta
     2041 tagttcttct tttctttctt ctagcgcctc taatctcccc tcgtttcgct tacgtagaaa
     2101 gattgccact acataagcca gaactaaaat gactgcaatt gcaaccatta aataaattag
     2161 ttgtccatta gacatatcaa actccttttt tacttgaaac aatcgtaacg attatatcat
     2221 attttttaga ccaagggaac tacttctccc tattttttag acatcaagtg tactatagac
     2281 agcattttct tcgataaact cacgacgagg ctctactcga tcccccatca acatatcaaa
     2341 gattttatct gcttctgcag catcatctac agaaactcta gccatcaagc gatgttcggg
     2401 atccatggtt gtttcccaca gctgatgatc gtccatttca cctagcccct tataacgctg
     2461 aatagtcggt ttggtacgac cttcactata acgggctaaa gcttcttgga gtttgatttc
     2521 ttgatctgca cccggctgga tatattcttt aatctcgctt ccaaccttga caccatagat
     2581 tggtggttgg gcaatataaa cataaccagc ttctaggatt ggtttcatat aacgataaat
     2641 caaggttaaa agaagggtac gaatgtgggc tccatcgaca tcggcatcgg tcatcaaaac
     2701 gagtttttgg taacgggctt tcgaaacatc aaattctgcg ccaaatcctg ttcccatggc
     2761 tgtgaaaaga ctacgaattt cttcgttagc tagaatctta tccatacttg ctttttcaac
     2821 gttcaaaatc ttaccgcgaa ttggaaggat agcctgaaac tcacggttac gaccagattt
     2881 ggctgatcca ccagctgagt ctccttcgac gatgaagagt tctgtttcag cagggttatt
     2941 agaagaacag tctgctagtt tccctggaag gttggaaatt tccaaaccag attttttacg
     3001 tgtgacttca cgcgcacgct tggcagccac acgagccttg gcagctaaaa tccctttttc
     3061 cacgatacgt ttggcaatct gtggattttc catgaggaaa tcggagaagg cttcactgaa
     3121 gaggcgattg gtaatcttga ccacttcgct atttcccaat ttggtcttgg tttgtccttc
     3181 aaactgtgga tttgggtgtt taactgagat aactgcagtt aatccttcgc gaacatcttc
     3241 ccctgttaaa ttatcttcat tgtctttcag taacttattc ttacgagcat aatcattgat
     3301 aacacgagtc agtgccgtac ggaaaccttg ttcatgcgtt ccaccttcat gggtatgaat
     3361 attattggcg aaactcatga cattttcatg gtaacctgtt gtgtactgca tggctacctc
     3421 aactgtgata tcatccatct caccgtctgt atagattggt gtatcaaaga ttacatcctt
     3481 gttctcgttg atatattcaa cgtaactagc aatcccacct tcataatgat aatgcttggt
     3541 ttgttccaaa ccttggcgct tatctgtaat tgaaatttga agaccgcgat ttagaaaggc
     3601 caactcttga atccgtttat ttaatttatc aaaatcaaag attgttgttt cagtgaaaat
     3661 ttctgggtcc ggtgtgaagt gaaccgttgt tccagttcta tctgtatccc caaccacctc
     3721 aaggtctgca acaacatgac cacgacggta ttcttggtaa tgaatcttac cgtttttatg
     3781 gacatgaacg tctaattgag tggaaagggc attaactact gacgacccca ccccgtgaag
     3841 accacctgaa accttgtatc caccaccgcc gaactttcct ccagcatgaa ggactgtaaa
     3901 gacggtctca acagcaggac ggcctgtttt ttcctgaata tcgactggga taccacgccc
     3961 atcatccaca acagtaatcg aatcatctgg ctcaataaaa acttgaatat ggctggcaaa
     4021 tcctgccaag gcctcgtcaa ttgagttatc aacaatttcc cagactagat ggtgaagacc
     4081 ttcttttgag gttgatccaa tgtacatccc tggacgcata cgaacagcct ctaagccctc
     4141 taaaacttga atttgactgg catcataatc ctgtgcctgc agatttttga tttcttctgt
     4201 catcttattc ctttttctta tatgtctaat acttgtctta aattcacgat actggtaaac
     4261 aagtgggtat caagtcctgc agcttgacct gcttcgatat caatcggccg atcaccaatg
     4321 acaagaccag agctaatctg atacttttct cttaaataaa gcatggattc gggatttggc
     4381 tttctcttaa agcctgagct agaagtcacc acttctgtaa aataagctgc tatagaggtt
     4441 ttttctaaaa tttccaaaac ctgatcattt cgatgagaga ccaaaaaatg acggccacct
     4501 tgatttaaaa tgtcttccaa taggtcagaa actccttcaa ataaaatcgg gtgttcaagc
     4561 tctctggctt cattttcctt gtacttttct aaaaaattct ctaaattggg agcgaatgtc
     4621 tcaatcgcaa aaggagtaga aacctttaaa gcttgataga cactgtcatg gtcttgtgtg
     4681 ataccataca gtgccaatgt ttcaacaaat gcagctgttg aagtttcata attatccagt
     4741 aaagttccac ctaaatccca gatgtaatcg tgatatttca tacccttcat tatagcattt
     4801 ttttatgaaa aaagcgatga tttccctgag ttttccacat aaaaaacgag agaataactc
     4861 ccgttttact gatttttacc aaagacatct cgatagagca ttcctgttcg taaactctct
     4921 gcgtcccctc ctagttgctc caccaactcg taggcaaagg caagggctgt tgaaggaccc
     4981 cgactggttg tcaactgacc atctaccact actgtttcct tgacgtagtg accatcaagg
     5041 atttgctctt gaacgccgtc ataacaagtg tatcgcttat ttttcaatat ctctgcttga
     5101 ttgagggcaa ttggtgccgc acaaatggct gctagtttct tcccttcttg ctcgaagctt
     5161 tgcaattctt gaatcaaggt ctgattatca cgtaaatgtg cagaaccagg catacctcca
     5221 ggaagaacaa tcatatcata gtctgataaa tctccatcaa agacatgatc tgctcttact
     5281 tggattgcat gcgaacccgt tacttgctct tcaaaaccaa ccatatcaca tgtgatattg
     5341 gctcgacgca agacatctac aactgtcaag gcttcaattt cttcaaagcc ctgagctaac
     5401 ataactgcta cttttaccat gattttctcc ttatttaatt tgttttaata caaccttttt
     5461 ccgtttgaat gggacttttc cgctcttttc ttcagctaaa ttagtctggt aactcatcga
     5521 taaaagcaaa ccaacaccaa tcaaattact gataatagct gatccccctt gcgaaatgaa
     5581 aggcaaggga atccccgtca aaggaagtag tccagtcaca gcaccgatat tctcaaagat
     5641 gtggaagagc aacatcataa tcaaacctgt ggaaatataa gtgtagaact ggttatttga
     5701 tttaagagta atcttcaaca tacggtaaat caacatgaga tagagggcaa taaccaggac
     5761 agagccaata aagccaaaat cttctgcaat aaccgtaaaa atcatatctg actctcgaac
     5821 tgggataagc agattcgaag cattaaatcc ctgaccaaat aagccaccac tcccaatggc
     5881 aatctgccct tgagcctgct ggtaagtcgt tgtttgggca aactcaaagg gattgagcca
     5941 agccaaaatc cgattaattt ggtaggtcgg cattccaatc tggtgaagaa aagctcgtcc
     6001 gtccttgcta ataaagatag ctaagaaacc agcaactcct gttacagcag tcacaaatac
     6061 tgggataata attttccaag aaacccctga taataaaacg attcctgaga aaatggctac
     6121 aaaaaccaaa gccgtcccca agtcactttg aagtgctaaa agaactagga ctggaatggt
     6181 aaagagaatc atccagaaaa ttaacaaaaa gtccagcgga accgtgcgtc tccattcctt
     6241 atgtttcttt gtaaattgga caatgacacg agccaacatg aggatatagg atatcttcat
     6301 aaattctgac ggttgaaata gggtaattcc atttattgat acccagtttt tggcacccgt
     6361 tgatgcaact aagcttggat tataaaatac aatcggcaag atcataagtc ccaagcctaa
     6421 aatatataga aagggggtca ccttccaaag aaattctgta ttaaagagca tgaccacaaa
     6481 accaatcaca agccccaagg caatccaggc gacctgctgc cctaaaatgg gcagaatatt
     6541 attgggataa tcatgactaa cggctatata gatagccacc acaccgatga ccagtagaaa
     6601 aaatactggc aagagcaaac tataatcgac tctagagtcg agagaacgtt tcatatgtac
     6661 taaccttata ctttcataca atactattta tcaaagttca ttaaaaaatc tatcaatagc
     6721 ctcgtcaact tcggatcgag agatggtttt aacagtcgct tcttctgcta gagatgctac
     6781 tatttgtttg ccgtatcgtt ttccgacgat tctcctatcc aaaataagag ttaaggaacg
     6841 ttggtattca cgtctcatac ttcttcccaa agcctgtttt aaacgaataa tgaccattgg
     6901 caattgataa tcataaaagg catttttccc ttcttgattc agttcttgat taatcttttt
     6961 cgtcaagggt tcttgaggat tttggaaagg aagcctcggt acaacttgaa tcacagaagg
     7021 atggcttgaa aaatcaactc cctcccagaa acttgctgca ccaagcaaga tttgttgttc
     7081 acctttttca aagcgtttct ttagctgatg aacatcccca tttttatact gggccaagtg
     7141 gctaactgta agtaaatccg atactgctag aagcatgtct ttagcggtaa agagaaccaa
     7201 aatcggttgc tggaaagctt gaatttccac tagtaaagca gctacctctc tggcatagac
     7261 ttctaaggag gtttctgtta ccagagggaa atctttgacc ataaccactt cttgttcctg
     7321 ttttccccga gattcaatct tgacaaattt ggcttcagga tagcctaaaa ggtctgccaa
     7381 agaaaccctc tgactaatct caagagtagc cgatactccc aagacttggc aactctcagg
     7441 aagtaaagag gacagaagag tacggcctga ttttgtagaa gaaatctgaa ttttcttttg
     7501 actcgtttca gttacttcaa gccagtaatc accttcagct gtaaaatagc gaaccagctc
     7561 atcaaagcct tccacttcta attctgaaaa atactgatgg agattgtcca gagaatctaa
     7621 gatatttttc ctagatttac cagactgaaa ttgttctatc aagtagagac actcaaagcg
     7681 aatactttct agtatccgtt gttgaaccct gttttcttct cctactaaag ccttatcaat
     7741 taaatcgata atagattgta tatcgtaggt ctcttgaagc agattttcta gagctaacaa
     7801 aatcttttgg acttcatcaa taatcagtaa acggtcactg acaaattcag gattatcttc
     7861 aagtctggtc acgagatagg catgattagt cactaaaagc ttgcaagtct ctgccctttc
     7921 ttgactacgt ttccaaaaat cttccgtcac aaataagctc tgggatgata aattcccatc
     7981 atgacgaagg tctgctagaa aatgttggta acggtagagt tgcccgattt catccaaatc
     8041 tcctgtctct gtctcagtaa gccagaccaa gacttgcatt ttaaagcgtc taaataagcg
     8101 attttcatca ttttcctgca aggaatgata aaaggcatcc aacttcagat aattttgtgg
     8161 tccctttaag ctatgaatat ctgtatggaa cacttccttg agacgtttac cttcttcttc
     8221 catgatttga ttttgaagaa tctttgtcgg aacactaaga acaatttgtc gctctttgga
     8281 ttgagataaa gcgggtaaga gatagccata ggttttccca atccctgtcg gcgcttgaat
     8341 cagagagaaa ggttcatctt tcaatagcaa gccaacctct ttagcaaaac tttcttgttc
     8401 ctccctcact tcaaggttca acagagaaat atttttagaa aagtcttgag atagttttcg
     8461 tagctccaga gaagctgccg ttttcttaaa atacagacct tgaacttgga ccaagtctgg
     8521 agaactcagg atagattggt tgcgataagt ttcctcaata accaggtaag actcatatag
     8581 gagagcgtca gccatttcca gcaagcgttc caagagacct ttaggaagct gggtcatctt
     8641 ttcccgtaaa aaaagaagta attctgctgt agcttgggca tctgaaaggg ctgtgtgtgc
     8701 gtgtttaaga ggaattccta attctcgaca caaaatcggc aagctatatt tttccagttc
     8761 agggaaaaag acctgggcca attcgaccgt atcaacacga gggtttctta gctcatagcc
     8821 ttcaaaaaat aaattttccg ccaagagatt agcatcaaac tgaacattat gggctacaaa
     8881 aatcccatcc tccaccaagt caaagatttt tctggcaact tgcgaaaaat caggtgcttg
     8941 cgccagacgt tggtctgtca atcctgtcag ttctttgata tgagcatcca agggttcatg
     9001 tggattgaca tccgtcgtat agtgatcgac gatttctcca tcctcaatca cgacaattcc
     9061 cacttggata attttagcct tactacctgt gctagttgcc tctaaatcca ccacaacata
     9121 gcgattacca atcgttttca ttataaaaaa ttataccaaa aaaagggcca tttggcacct
     9181 gcaaacatag gataattttc aaactcaaga acactacttt tcttagaaat cccatcgaat
     9241 agagcattct agcctgctga tttttttaaa atagtgaaga aatacgaaaa attgagagaa
     9301 gaaatgtttt catcttcctt ctctcaacga atcatgtatt ttatttaacc atgtcaggat
     9361 cgtagtcata aaatcctgta tcaaggaaac ggtttttagg gtgaagttta cgaacttctt
     9421 catccagcaa gaaggcttgg tcatggctaa agttgccctc ttgaatcaag ctacgcgctt
     9481 ggataaagag ttttgcaatc tcaacaaagt tctgaccagg agtgtagagc ccttctagtt
     9541 tccagcgagt aaagccatgc tccaccaatt ctgttaattt gatcatcaaa tcaaggtcat
     9601 tgttggcaaa gatatgggtc ccatgattat cttcaaaaat ggaatagtgg ctctctggat
     9661 cacttggctc agccaagaag aggtcacgtt tatgcgtctt ttcatcatcg atatgtgtaa
     9721 agttatagta gttttgcaag agtggacgtt tagaatgatg gatgacgcta gcaccgtaaa
     9781 ccaaaacttc agcaggaatt tccaaaatct ctggcatttt gaaaagttca gctgatggaa
     9841 tttcacgcgc caaaacagcc tcagatgcgc cagccttttg tccccagaag ttaatctgac
     9901 gactgctagt taccatggtt gaagcatcgt agatggtctt aaatgaataa ccatcgcggt
     9961 taactacgta aaagacgcct gcatccccaa tcgtaatata gtctgtcttg atttcttcca
    10021 agaagtctaa gaaaggcttg atacggtcca tcatatcttg gtgcatgaga gcattgacca
    10081 caacgatcaa ttccttacca gcatcatgaa ccaacttagc gatttcacgt aattggtcat
    10141 aactaaaggt cgttggcaga cgaaggccaa aatctttctc accgacatag atacggtcta
    10201 cgccagcttc gagtagttgt tcaacttgtt caatactttc agcagttgct gtaatgacaa
    10261 tcttttccat aagaaaaatt ataccacatt tctccaaaat cagccatgct ttgaaaatga
    10321 aaagggactt tattcttttt gtaacctttc tgtaataatt ctctgccgaa aaaatgataa
    10381 aataggtatg tactgttaag gagagaatca tgcccgtaag aaaattacaa tcctatgagg
    10441 tagactatca agaagaatta aaccagcagc ttcctcatta ccaagcttat acacctgaag
    10501 cgcaatctga tgccaatctc aaggaaatcc tattttttat taatatcgct gttttttgta
    10561 tctgtattgc tatctttagt tttatctttt tagcattaaa attatcaact gctcttgcct
    10621 ttgccgcagc aatcggattc agcttacttg ttttaaaagt tcaacggtct attatcaaac
    10681 gaaaacgtag aagataaatc caaaatatcg ccccaattgg agcgatattt ttattttttc
    10741 tttttagatg gtgtctgaat ggcaatcttc acttcaaaga ttgttccctt gggtttatta
    10801 tctttgacag taacagttcc ttttagagca tctacaattt gcttggctag ggataatcct
    10861 aaaccaaaac cacctttttg ccgggttcta gctttgtcta ctcgataaaa acggtcaaaa
    10921 attttctttt tatcttctgt cgaaataccg attccattat cagaaacaag taaataaaga
    10981 ttgcgatcgg tcgccgagat aagaaaatca atttcaccat cctcctcagt atacttgacg
    11041 gcattatcga aaagaatggt catcagttgt ttcagaagaa gctgatctgt gacaattgtt
    11101 cgatggatac gattttcaaa acggaagaca cgattatttt ccgaagcaat catctcatag
    11161 tttgtgaaag ttgtattaaa aaagctagtt ggaacttctg caagctccgg cttaatccca
    11221 tcatctctcc gagctaagtt cagcaagctt gtcgttaaaa aacgcatatt tcggacttct
    11281 tccaaactcg atgcaatgct ttcgctcaca tccataatgg tagcttctgg cttacgaaaa
    11341 agggtctcta agcgattttg caaaactgcg agtggagttc gtaactcatg actggcattt
    11401 tccacaaaag actgttgctt ctgcatactc tcaagcaggg gcctaacact gaccctagct
    11461 agatagagac tggcaagtaa agacaaaatc cagaaactag ccatcacgac cacaatcaat
    11521 tgctcatgct tttgactagc ctgctccaac tgactggtat taatcaagac agcagcatac
    11581 ttgatattgg ttgaaaccga actaatattg gtctccatca aaatcacacg atagatttct
    11641 tcctgcccat agctattaaa aacctgaatc tggtagatat gtcctagttc tttcttttct
    11701 aacttaatct tatccaagcc caaaaatcga tttccagaaa gaagttgagt aaagtcttta
    11761 tcaaagagaa tgacttccgt attggaactg acattaggtt ttatttctac tttactagcg
    11821 tccgccctag cattttctaa atctttaatc tcttctgttg ccctatttat agccagctga
    11881 ataactgctt gaggattttc actcagtcca tgaagcttat cgtccaccga agtataaaga
    11941 ctcgaatgca tgacttgtaa aataatcaga gtcattgtag aaaaaatcag ggtgaagaca
    12001 ccgaagttgc ggataaaata actaaagtca tccgcatacc atgttttttt aagtttactg
    12061 aacatctttt aaaagatacc caacactacg caaggtttgc aaattctctg caaaagtggt
    12121 tccctttaat ttcttacgga cttttgaaac atagacttcg acaaccgaaa tcgttgtatc
    12181 actatcaaat ccccatagac ggtcaaaaat ctgcgtctta ggcaaaatca cattttgatt
    12241 ttgaaggaaa taaactagta aatcgaactc tttccccagc aattcgacag gagtatcttc
    12301 aactttaacg gtattggttg ataaattaac cacgatattc ccataagtca aggtgttttc
    12361 attaaacttc cctgaacgtt tgagaagggc ctgaattcgc attttaagtt cttctaggta
    12421 gaaaggtttg gtcagataat catccgctcc cagttcaaat ccatgtccct tgtcatccaa
    12481 actttccttg gcagtcataa tcagaactgg tgtcgtaatt cccttttcac gcaattcttt
    12541 taagacttgg aaaccatttt tttctggcaa catcaaatcc agcaaaatca agtcatagac
    12601 accactctca gcttcgtaga gaccttcttc tccatcaaat acctgcataa catccgcaaa
    12661 atcgtctaaa aagtcaaata ctgaatttga cagacctagg tcatcctcaa ccaataagat
    12721 ttttatcatg agaaactcct ccttattaaa actattatac caaatttgcc ttaaaaaaaa
    12781 ctcaactctc tgcattttac atgagatagc tgagttttct t
//
LOCUS       DBEJAQ010000050        12641 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.050, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000050 DBEJAQ010000000
VERSION     DBEJAQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.050"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(118..1215)
                     /locus_tag="ACL297_001692"
     CDS             complement(118..1215)
                     /locus_tag="ACL297_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGR0071921.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILAQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNLSIAQQNFGRVPIRIETN"
     gene            complement(1225..1965)
                     /locus_tag="ACL297_001693"
     CDS             complement(1225..1965)
                     /locus_tag="ACL297_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGR0071922.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(2285..2638)
                     /gene="rsfS"
                     /locus_tag="ACL297_001694"
     CDS             complement(2285..2638)
                     /gene="rsfS"
                     /locus_tag="ACL297_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGR0071923.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(2651..3151)
                     /locus_tag="ACL297_001695"
     CDS             complement(2651..3151)
                     /locus_tag="ACL297_001695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGR0071924.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(3152..3745)
                     /gene="yqeK"
                     /locus_tag="ACL297_001696"
     CDS             complement(3152..3745)
                     /gene="yqeK"
                     /locus_tag="ACL297_001696"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGR0071925.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(3745..4374)
                     /locus_tag="ACL297_001697"
     CDS             complement(3745..4374)
                     /locus_tag="ACL297_001697"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGR0071926.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(4426..4737)
                     /gene="yhbY"
                     /locus_tag="ACL297_001698"
     CDS             complement(4426..4737)
                     /gene="yhbY"
                     /locus_tag="ACL297_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGR0071927.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(4951..6057)
                     /gene="yqeH"
                     /locus_tag="ACL297_001699"
     CDS             complement(4951..6057)
                     /gene="yqeH"
                     /locus_tag="ACL297_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HGR0071928.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFITGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(6060..6587)
                     /locus_tag="ACL297_001700"
     CDS             complement(6060..6587)
                     /locus_tag="ACL297_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HGR0071929.1"
                     /translation="MAIENYIPDFAVEAVYDLTVPSLQAQGIKAVLVDLDNTLIAWNN
                     PDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVEKFGIDYVYWALKPFTFGIDRA
                     MKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKPLVQHDSIKTQINRTRERRVMR
                     KITEKYGPITYKKGI"
     gene            complement(6602..7510)
                     /locus_tag="ACL297_001701"
     CDS             complement(6602..7510)
                     /locus_tag="ACL297_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGR0071930.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDKPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(7567..8127)
                     /locus_tag="ACL297_001702"
     CDS             complement(7567..8127)
                     /locus_tag="ACL297_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGR0071931.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            8266..9475
                     /gene="sstT"
                     /locus_tag="ACL297_001703"
                     /pseudo
     CDS             8266..9475
                     /gene="sstT"
                     /locus_tag="ACL297_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            9543..10496
                     /locus_tag="ACL297_001704"
     CDS             9543..10496
                     /locus_tag="ACL297_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGR0071932.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            10639..11019
                     /locus_tag="ACL297_001705"
     CDS             10639..11019
                     /locus_tag="ACL297_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGR0071933.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETVAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKILASLQKTVEESLEAGLDFLI"
     gene            11118..11339
                     /locus_tag="ACL297_001706"
     CDS             11118..11339
                     /locus_tag="ACL297_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGR0071934.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            11356..11670
                     /gene="trxA"
                     /locus_tag="ACL297_001707"
     CDS             11356..11670
                     /gene="trxA"
                     /locus_tag="ACL297_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGR0071935.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            11975..12508
                     /gene="queF"
                     /locus_tag="ACL297_001708"
     CDS             11975..12508
                     /gene="queF"
                     /locus_tag="ACL297_001708"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HGR0071936.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEDLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         3516 a         2873 c         2501 g         3751 t
ORIGIN      
        1 aggaaactag ccgcaggctg tacttgagta cggcaaggcg acgttgacgc gatttgaatt
       61 tgattttcga agagtattat tcgtaaaaaa tctcaaaaag tccacctttc ggtagactta
      121 gtttgtttct attctaatcg gcactcttcc aaaattctgt tgtgctatac ttagatttcc
      181 tagttggtaa atctggtctg ctttttgggt catagcatcc caaggttctt tgccaattcg
      241 gctgactaga ctgacctgac ctttcaaaga cttgagatgt tgcctgcctt tttcagtaaa
      301 tccaaggaca tgaatggctt ctggcaagac attttctcta gcctgcgcca agatatagga
      361 caagaggcgt ctgacacgcg ccttggtgta acgtttggta gtcactaact cgaccaattc
      421 ttccacagac tgggctgttt taatagcttc tttaatgcgc acagccattt cttgattgac
      481 ctgatagatg gtggttaggt cgggatttga caagatttga tagcggagca agggaaaata
      541 gtcttcccag atcaccttac tggcctgctc aaatagggca acagaaggca taaaacgttc
      601 taagaaatct tggtccttct gatgttgacg gagggctgtc gccgaggcaa agtccacatc
      661 tttattcaca gaatggtaac ctgccccctg acgctgaatc ggatgcagct tgatattccg
      721 tcctgcaacc gccttggcat aggccagagc aagaacatga ttgggggtat tacctgaaaa
      781 atcaagacca gcaaattcct tccacatagc ttgggttttc tggggatagg aaagggaatc
      841 aggcagattt tccacaaatt tctccatctc agtacctttc tctgtgtata agtcagcgat
      901 tttctgataa tctcgaactt cctctgtccc aaaaactaga ctatcaatgc ccaagcgatc
      961 caagatatcc acagctcctt gaccaaagaa atcagctgcc tgaacactga ctaaaaaggg
     1021 caattccact accaagtctg cgccattttc cagcgccatc tgggcccttg tccacttatc
     1081 cacaatagca ggctctccac gctgcatgaa attccctgac atggcaacaa ttttcagtcc
     1141 ctctgcctga tccagcaggt atttatggcc attatgaaaa ggattgaact ctgcgataat
     1201 acctgtgatg gtcatgatgt tctcctactt ctgcgccaca aaaaaccaac gggtgctagt
     1261 ttctgttggc tccttatcct caaagtctgc atagagtttg aaggacttaa atccagcctg
     1321 ttccagcaag atatcatagg tcaagatctc ataggtcctc tcctcatgta cttcatcgtg
     1381 gcgactaaag gaaccgtcag cctccttgat aaagaaagtc agctcatgca cgatggagtg
     1441 aggagcttcg tcctcatatg tatcccaaag catggcaaaa tcttccgcat tttcatggta
     1501 ggaatagcca gggaatactt catctgtctg gtaggtcgag tgcacgtcaa agatgaaaac
     1561 tccctcttca ttcagcgcat tgtacacgtc cttaaagacg tcccctactt ccacctcatc
     1621 ctgcatatag cagatagagt ccgaataaca cgtgacaaaa tcgtatttcc ctgccttgga
     1681 caaattcagc atattacctt ctataaaagc aatcttttgc ttggctgaag ttgctctctt
     1741 ctccgcaatc ttcaacatat ccgcactcaa gtcaagtcca gtcacatcaa aaccagcctg
     1801 agagaagcgc actgattgaa ttcctgtccc acaagccaat tccaagagtt tctttctctc
     1861 cttggtctta ggcaaatgac gcagagaaaa attcgtccat ttgtcgtata aactatcgtc
     1921 catgaccgca tcatagaccg ccgcaaaggt ttcataagtc gccataaaac atgataccaa
     1981 gtccgcccgt aattcgatag aaaattagga gccttacgat ggagccgagc tccaaggaag
     2041 gctatctatt ttccgagaat tacaggacgg gttcaaatcc tttctaagat acaaagagcc
     2101 ttaaaagcaa agagtacata ggaaactttc ttccctatcg tcagatacaa agaaagttac
     2161 tttttatctc agcttttagc tcgtgttcag tttcgaaatg aaacaaacac gaggcccttt
     2221 acctttctat caatttttct aaaataagca aagaaaccca gttgactaca actgagttca
     2281 tcttctaagc aagagcttct gaaatatcta ctgaattcgc ctcatgccat agcttctcta
     2341 gattataatg ggcacgcatt tcttctgaaa agatatgcac gacaacagca ccgaggtcca
     2401 gtaagaccca gcctccagct gcatcgcctt cgacatggct acctttaaag cctgcttgag
     2461 ctactttttc acggatatta gctgcgatag cgtccaactg acggctattc attgagctag
     2521 tgatgacaaa gtagtccgtc acactagtca aatcttgtac atcaagtgcg aggatatcct
     2581 ccgcacgttt ctcatcagcc gctttcacga ctagttctag taattctttt tcgttcattt
     2641 aatcctcttt ttagaaaata tgtttgtaat ctagaacatc tgcaaagcgc tcctcccaaa
     2701 tgtcctcata aaattcatta atcgtttctg ctggaatgtc atccccatca aaggttgtga
     2761 cagcaccttc tggaacaata agctgatagc catattcaaa ggcaaccttg acagaggtat
     2821 ccacacaata ttctgtctgc ataccacata aaactaattt ttcaatcccc tgcttatcca
     2881 agtattcttt taagccagtt tctttgaaaa tactgttata cttcttctga aagacctttt
     2941 cagcaggttt tcgatttaaa agcgcagata actgccaatc ttctgatgtt tgagcttcag
     3001 agttctcaat atgttgaaca tagataattt caatattctt gcttctagcc tggttttgta
     3061 aataagaaat tttttccaat agactttttg tctggaaccc agtttctact aaaacattct
     3121 gaacatcaat gattataaaa gccgtcttca tttagtcctc tttcaaatag tgcacaaagg
     3181 cgttataggt ttcaagggtt tggggataga tggggaatcc ctgatgagct agatactcca
     3241 cggtacgagc tgtttcgtag gccaccgcct tattgagcga tagacttgca atctcacgcg
     3301 ccacatccac tccaggaaat gcccgattgt gctcgatata gtctgcgacg tagatgacct
     3361 tatctaggtc tgtcatttga ccagctccga ctgtatgaat ttcaatggct cgcagaattt
     3421 cagaatcatg caaatccaaa tcttcctgaa ttttgtaaat tccgaccata ccatgccaga
     3481 cattgttgcc ccagtttttg aggtcagggt ctagctggta acggtcaatc aaggctagaa
     3541 attcctgatc tgacaacttt ttagcatagt catgaagaag acctgctaga cttgctttct
     3601 caacatctac accaaagcgc tgagctaatt ccatagcggc acgctccaca cctagacaat
     3661 gggttagacg tttctcaggt agaagctctg ccattttttc caacaaggcc tcacgggagc
     3721 agttgatata gtcttgatag gacatcagta gagcccctcc ttctcgatgt agtctagcac
     3781 tggctgaggt aggagaaagt tgggtttccg accttgggca aggaagtccc gcaccatgct
     3841 ggacgagata tccatgagcg gtacgtccac ccagataact ggataggaag tccctacctt
     3901 gtagcgtgga cgctgaaccc ccacaaactg aaccatgtca accagttcat caattcggta
     3961 ccacttaggc agatagtcaa ccatgtcggc accgatgata aagtaataat ccgtatctgg
     4021 attcttctct gtcaaaatct tcatggtgtc gtaggtgtag gaaataccct tgcgctccaa
     4081 ctcaatggtt tcaatgacta ggccgtcaat tccctcaatt gccaactcaa gcatcttgag
     4141 acgatggtgt tcagggatgg tttccttttt atcaacgtga ggaggttggt attcaggcat
     4201 gagcagaact tgatccagtc ccaactgttg ccgtacttga tccgcaacaa taagatgggc
     4261 attgtgaaca gggttaaaat tcccccctaa aatcccaact tgtttgcgtt ttttctcctt
     4321 gatttctggc tccaactcta ccttggtaaa gggagtcaat agttcgattg tcataggcta
     4381 gtccccttta tttctctatg aaaacagtta tttggagttt cggtcttaga tttctttgac
     4441 tttcttagaa atcttgcgat tttctttctt gctagattgt ttaaacaaaa tcaagatgcg
     4501 tcctattttt tggactgtat ccacaccgat ttcttcttcc aaaatttcag ctacttcgtg
     4561 gatgttttca tctgtgtttt gtaagagagt aaccttgatt aattcacgcg catcaagagc
     4621 ttgacggacg ctggttttga tttggtcgtt gagtccattt ttcccgattt ggatgatagg
     4681 tttgagggtg tgtgcctggc tgttgaggaa ggcacgttgt tttgatgtta atgacataat
     4741 tttcttcctt gttttttgat agttatttta ggtggtgagg gacggtcgga actaattttc
     4801 caaagatttc atctttgaaa aatggatttc ctgacctcac aattgagcct tggtctcaat
     4861 tgtgcccctt gccaatctac agattggcaa tcacaccacg gcaataacta tcgctatatg
     4921 agctcacttt gttcgctcag cgattttgtt ttaaataatt gcttttcgtg tgacgacggc
     4981 gacgccttct ggtgcccaga cggcgacttt tgctgtgcct gttacacgaa tccagcctag
     5041 gcctgagatg actaggtctg tcttatcttt aatggtaaag acatgctgga ctagccttgg
     5101 gaaatcttct ttttccttgc tatttggtgg tgtcagaaga gttcccaggt gcttatcgta
     5161 gaaagcacta gctccttcaa gcttgctacg atggagtttg agttcattgt caaagaaagc
     5221 agtaaatcct tgcttttctc ctgttataaa gtcaaagcgt cccaaaccac ctaaaaatag
     5281 ggtttgctca ggattaagct gataggtctt aggcttgatt tcctttttag gactgacata
     5341 cttgaggttt ttggccgtca aatagtgagc catctggtgg cggtggataa ttcccggcgt
     5401 atcgtaaata taagatccgt cgtcaagcgg aatctctatt ttgtccaagg ttgtccctgg
     5461 gaagcgtgaa gtagtgatga cattctgatc acccgtgatt tcttggataa tagcattgat
     5521 tagagttgat tttccaacgt tggtcacacc gaccacatag acatcgcggc ccttacggta
     5581 gtgttcaatc ttgtcaatga cttccttaat ggcatgttta ttttgtgctg aagttaggac
     5641 cacatcgact ggacgaagac cttcttcatg ggcacgtttc atgagccact ggctaatctt
     5701 accagactta actgacttag gaaggatatc ttttttattt cctaccaaga ggacatcatt
     5761 gcccgagacg aaacgtggta aacctgggat gacagatcca ttaaaatcaa agatatcaat
     5821 gacattgacc actaaagcat cactgtctcc cacctcgtgc aagagcttga ggaaatcatc
     5881 gttcgtcaac tggacatctg tgatttcatt gtagtggcgg agacggaaac agcgttggca
     5941 atagacttcg ccagtctcca aacctttttc aagtgccgac tggggggtaa aaccaagacc
     6001 agctttatct gtcgtctgaa tggttgctcc acaaccaata cagagaattt cttccatagt
     6061 taaattcctt ttttatatgt aatcggtccg tacttttcag tgatttttct cataacacga
     6121 cgctcacgag ttcggttaat ctgcgttttg attgagtcat gttggaccaa gggtttgact
     6181 aaaattgacc gaatccctgc acggtgggct gctcgtatat ctgtcatgag ctggtcacca
     6241 accatgacca cttccttttt gtcatagtgg aattccttca tggcacggtc aataccaaat
     6301 gtgaagggct tcaaggccca gtaaacgtaa tcaatcccaa atttctcaac tgctcgttga
     6361 acgcgttttt tggtgttatt tgacactacg ataatgccaa tacccgcgtc ccgaaggtca
     6421 tgtagccatt gcttcatctc tggcgttcca tcagggttgt tccaagcaat gagggtatta
     6481 tccaaatcga ccaaaacagc cttgattccc tgcgcctgca ggcttgggac tgtcagatca
     6541 tagactgctt ccacagcaaa atctggtata taattttcaa tcgccattct ggctcctttt
     6601 cttaactttt tttcgcaatt ttccttagta acaaggataa aacaatccac aaacctgcac
     6661 tagccaaact gatatagagc caagcatggg gcttatctgt taaaggtaag ggaacattca
     6721 ttccgaaaaa gcctgtcacg actgccaaaa cagctagcaa gactgaaatg atagtcaagg
     6781 ttgtcaaatt gtcattcaga ttattgttta gaatattgtt gtaagaggct gaaagctgct
     6841 gtaaaatctg agagattagg tctgtcatgg ataccagctg atgagcctca atcatggcat
     6901 catcaaactg ttctctctca atctcatcaa aactacgata caaggcatga ccttgaatat
     6961 gctctaacaa aatccgattt tgtttggcag ctgccgtcag ataaaccata ccagtctcca
     7021 aatcagacag ggcaaagagg tttttcttgg tagttcgctg gcgcaagagg tcattgacct
     7081 catccctact cttgtccatc tgctcaatga caggatagta ggcattgctg atgatttcca
     7141 gactggcaaa gagaaactta taaatcgaaa gcgtgtcatg gttctccaga taacgagtca
     7201 tctgttcaat gacataggcg ttcttggtat tactaatggt aatcaggcga cgatgctcga
     7261 caataaaggt catgggaaaa gtctcatagt aggccttgtc cttttttacg tctaggacat
     7321 tatagataaa ggtaaccgtc ccactttcac ggtggtagtc catgtgggcg cgctcgtttc
     7381 tatccagtgc gtattcaatg gtttccttgt ccaaaccgta aatttcagaa aggtcttcta
     7441 gtttattcaa ctttcctagg tctaggtcta tccaggtaca accattgccc aactgctttt
     7501 ctaaaaccat cttttctcct tattaaactc cctctattgt accacaaatt acttaaaata
     7561 acaggtctag tctgtgcgtg agaaattgct aacaactgtg atatttcggt tagggacaaa
     7621 gtgaagggct tgctcctcgc tacgaagctg tgtcgtacga attcccacac tgacaacctt
     7681 acccgataca gtaatcggtc catttgtcag aacgacctca tctcccacat ccagttgacg
     7741 ttcaaagagg atgaaaaagc cattgatgac atcagacaga aagccttggg ctcccatacc
     7801 aatcgctacc ccagcaatac cagctccagc cagcaaacta gaaactggca aacctaaaat
     7861 cgacaaaatg cagtagagta aaaagaaata tagcgtataa ttaaacacat tttctagtaa
     7921 acgtgagatg gttttttggc gtccgacatc atgacgagac atttttagag aaggtttgac
     7981 aattctctgc accatggtat gaagcatttt tttagcaata taaaatacaa tcaaaagcag
     8041 taaaagagaa atgaccttgg ttagaatatt ctcgataatg gttgtcacat ctagcttttc
     8101 aatataagcc tgaataaatt tttgcatata aaactccttt atttcattat accatatttt
     8161 catcagtctc attctccata aatattcaaa aaatagacag aaaattcttg attttgtcta
     8221 tcatttatac tataatcata atcactacac aaaaaggaga ttattatgaa aagaatcatt
     8281 catgcttgga ataaggcaag ccttgtcaag cgtatcttga ttggtatgct ttgtggggaa
     8341 tcctaggact gacccttcct aatctctcag ggattgggct actcggggat ttattcgttg
     8401 gaggtctgaa agctgttgct cctatcctag tctttgccct cgttgccaat gccctttccc
     8461 aacatcaaaa gggacaagat agcaatatga aaactgttgt tttcttgtat atcctatgat
     8521 agggactttc gccgctgctc ttgtagctgt actagcaagt ttcatcgtcc ctattgaaat
     8581 taccctaaat agtgccaata ctgaaattgc accaccagat gggattgggc aggtcctcag
     8641 caacctcttg ctcaaactgg ttgacagccc agtcaacgcc ctgcttactg ctaactatat
     8701 tggaatctta tcttgggcag tcatttttgg aatcgctatg agagaagcta gtaaaaatag
     8761 taaagaattg ctaaaaacta tcgctgacgt gacttctaaa attgtcgaat ggatcatcaa
     8821 tctggctcca tttggaatcc ttggtcttgt ttttaaagcc atttctgaca agggagtcgg
     8881 aagccttgcc aactacggta ttttattggt tccattagta acgactatgc tttttgttgc
     8941 ccctgtggtc aaccctttga ttgccttctt ctttatgaga cgcaatcctt actctctagt
     9001 ttggaactgc ctccgtgtca gcggtgtgac agcctttttc actcgtagtt ctgcgactaa
     9061 cattcctgtc aacatgaaac tctgccatga ccttggactc aacccagata cctattctgt
     9121 ttctatccca ctcggttcta ctatcaatat ggctggagta gcgattacca ttaacctttt
     9181 gacccttgct gcagttaaca ctcttggaat tcctgttgac tttgccacag cctttgtcct
     9241 cagtgtggta gcagctatct catcctgtga tgcttcaggt attgccggag gttccctcct
     9301 tcttatccca gttgcttgta gccttttcgg tatttctaac gatattgcca tacaaattgt
     9361 tggggttggt tttgtgattg gtgtcatcca agactcatgt gaaacagccc ttaactcttc
     9421 tacagatgtc ctctttaccg ccgttgccga atacgcagca acccgtaaaa aataactcat
     9481 caaggcaagc ctgctcatgt cttgtctttt acgcttttat tctaacttat taggaaattc
     9541 ttatgtctat tagccaacgt acgaccaagc tcatcttagc tacctgtctt gcctgcctgc
     9601 ttgcttattt tctcaatctt tcgtcagcag tttcggctgg aattatcgct ctcttgagcc
     9661 tatctgatac gcgtagaagt actttaaaac tggctcgcaa tcgtcttttt tctatgcttc
     9721 tagctctggc tatcggtgtt ctagcttttc acttgagcgg atttcatatc tggagtctcg
     9781 gcctctatct ggccttctac gttcctttag cctacaagat gggctgggaa attggcatca
     9841 caccaagcac tgttttggtt agccatctct tggttcaaga gtcaacctct ccagaccttc
     9901 tagtcaatga attccttctc tttgctattg gtacaggatt tgccttgctt gttaatctct
     9961 atatgccttc acgagaagag gaaatccagc actaccacac gctggtggaa gaaaagttaa
    10021 aagatatcct ccagcgcttc aaatactatt tatccagagg agacggacgc aaccgagcac
    10081 agctggtagc agaattagac acgcttttga aagaagccct cagactggtc tatttggatc
    10141 actctgacca cctctttcac cagaccgact accatatcca ctactttgag atgagacagc
    10201 gacaaagtcg tatcctgaga aacatggccc aacagattaa cacttgtcac cttgccgcca
    10261 gtgaaagcct gatcttagcg caactctttt caaaaattgc aggtcaactg agccagacca
    10321 atcctgcttc tgatttgcta gatgaaattg aacgttatct ggaagtcttc cggaaccgca
    10381 gtctgcccaa gacaagagaa gaatttgaaa cccgcgccac ccttcttcaa ctcctacgtg
    10441 aagccaaaac cttcatccaa gtaaaagttg atttttacca aaaatataga cagtaaaaaa
    10501 taaaaccact aaaaaacaga gcttgccagc attcttgaaa agtagtataa ttattgcatg
    10561 cgcaatcatc ttgtgacgca gagactgccc gttaatggac tttcttctat ttatgactct
    10621 aaaaagaaag gagacactat gacctatttg gaaaaatggt ttgacttcaa tcggcgtcag
    10681 aaagaaattg aaagtctctt ggaagagacc gttgcccaac agagcgagca aagtctgacc
    10741 ttgaaagagt tctacctgct ctactatctg gacttggctg aagaaaaatc tctacgccag
    10801 attgacctgc cagataagct tcatctgagc ccgagcgcag tttctaggat ggtggctcgc
    10861 ttggaagcta aaaattgcgg tctactcagt cgcatgtgtt gccatcaaga tagacgctct
    10921 agttttatct gcctgacaaa tgatgggcaa aagatactgg cctcgctaca aaagactgtc
    10981 gaagaaagct tggaagcagg tttggatttc ttgatttaag atgattttag aaaaagttgc
    11041 gtgcgcaatc atttttcttg acattctctt ttataaggag taaaataaag ttagcattaa
    11101 acaaaggagt tttaaatatg attgaaatta cctatataga tgccagcaag aacgaaagaa
    11161 ctgtgacttt cgagtcttat gaagactttg aacgttcgca acaagcttgc cttatcggcg
    11221 tcgcagacta ctaccctgtc caaaaattaa cttacaaggg tcataatttg gactaccatg
    11281 ggacttatgg agatatcttc ttctatctca tgaaacaaga tttaagccaa tataactaaa
    11341 aaaaggagaa atacaatggc aaaagcaatt acagatgcaa cattcgaaca agaaacaaaa
    11401 gacggtttgg tcttagtaga cttctgggca acttggtgtg gtccatgtcg tatgcaaggt
    11461 ccaatcttgg acaaattgtc tgaagaactt tcagaagatg tcttgaaaat cgttaaaatg
    11521 gacgttgatg aaaatccaaa cacagctcgt gcttttggaa tcatgtctat tccaactctt
    11581 ctcttcaaaa aagacggcca agttgtcaaa caagttgcag gtgttcacac agcagaacaa
    11641 atcaaggcca tcattgctga attgagctaa tcaaacgaga gaccaagtac tttctttggt
    11701 ctcttttttc ttgccctttg ccatttttca aaaaatatgc tagactatag tagattgaaa
    11761 ctagaatagt acacctttac ttctaaaaca ttgttagaaa tcgatttaac tgtcctgatc
    11821 gatttgtcct gttcttattt cattttacta tataagtaga atattacgat gtttgggata
    11881 tgccatcgct aaaaaaactc gatttggtat tttttagctc cctaatagga gctttttgca
    11941 tgctctgaac atttcccatt ttggaaggag tactatgaaa cgtcaatcag ccttggtcgt
    12001 ctttagtggt ggtcaagatt caacaacctg ccttttctgg gttatgcaac actatgaaac
    12061 agtcgaagct gtcacctttg cctacggcca acgtcatcac ctcgaaattc aaattgctag
    12121 agaaatcgct aaggaacagg gcattcgtca ccatatcctc gatatgtctc tgctgggaca
    12181 aatcactgct cagccagact ttgcgacgat tcatatttcc tacattcctg acaagctctg
    12241 tgtcgagtca aaatccctca aactatatct atttagctac cgaaatcacg gagatttcca
    12301 cgaaaactgt atcaacacca tcgggaaaga cttggtcaac ttgctagacc ctcgctattt
    12361 agaagtctgg ggaaaattca ctccgcgcgg tggcatttca atcgacccct actacaacta
    12421 cggtaagcaa ggaactaagt atgaggactt ggcagaacaa cgcctcttcc aacacgacct
    12481 ttatccagag aaaattgaca accgctaaag tcatactcaa tgaaaatcaa agagcaaact
    12541 aggaagttag ccgcaggttg ctcaaagcag tgtttgaggt tgtagataaa actgacgaag
    12601 tcagctcaaa acactgtttt gaggttgcag atagaactga c
//
LOCUS       DBEJAQ010000051        12365 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.051, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000051 DBEJAQ010000000
VERSION     DBEJAQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.051"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(9..1223)
                     /locus_tag="ACL297_001709"
     CDS             complement(9..1223)
                     /locus_tag="ACL297_001709"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGR0071937.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1379..1673)
                     /locus_tag="ACL297_001710"
                     /pseudo
     CDS             complement(1379..1673)
                     /locus_tag="ACL297_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1835..2287
                     /locus_tag="ACL297_001711"
     CDS             1835..2287
                     /locus_tag="ACL297_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGR0071938.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2322..3710)
                     /locus_tag="ACL297_001712"
     CDS             complement(2322..3710)
                     /locus_tag="ACL297_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGR0071939.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3776..4738
                     /locus_tag="ACL297_001713"
     CDS             3776..4738
                     /locus_tag="ACL297_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGR0071940.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4944..5954)
                     /gene="ccpA"
                     /locus_tag="ACL297_001714"
     CDS             complement(4944..5954)
                     /gene="ccpA"
                     /locus_tag="ACL297_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HGR0071941.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLVKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6378..6977)
                     /locus_tag="ACL297_001715"
     CDS             complement(6378..6977)
                     /locus_tag="ACL297_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGR0071942.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLAFQADVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(6978..8075)
                     /locus_tag="ACL297_001716"
     CDS             complement(6978..8075)
                     /locus_tag="ACL297_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGR0071943.1"
                     /translation="MLERLKSIDYMYWASLIFMIFPILPVVTGEIPSWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERAEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVSPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8075..8812)
                     /locus_tag="ACL297_001717"
     CDS             complement(8075..8812)
                     /locus_tag="ACL297_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071944.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAMFSFPAVLHTDKINNWQKTLRHTPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHFVRGVDMPLGSWIGAAFLLIVGSVAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGGINL
                     SATVYILVFSAVLFGLTIYLQGHKENA"
     gene            complement(8814..9698)
                     /locus_tag="ACL297_001718"
     CDS             complement(8814..9698)
                     /locus_tag="ACL297_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071945.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9685..9879)
                     /locus_tag="ACL297_001719"
     CDS             complement(9685..9879)
                     /locus_tag="ACL297_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071946.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(9876..10082)
                     /locus_tag="ACL297_001720"
                     /pseudo
     CDS             complement(9876..10082)
                     /locus_tag="ACL297_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10671..10744)
                     /locus_tag="ACL297_001721"
     tRNA            complement(10671..10744)
                     /locus_tag="ACL297_001721"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10708..10710),aa:Pro,seq:tgg)
     gene            complement(10760..10833)
                     /locus_tag="ACL297_001722"
     tRNA            complement(10760..10833)
                     /locus_tag="ACL297_001722"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10797..10799),aa:Arg,seq:acg)
     gene            complement(10843..10928)
                     /locus_tag="ACL297_001723"
     tRNA            complement(10843..10928)
                     /locus_tag="ACL297_001723"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10892..10894),aa:Leu,seq:taa)
     gene            complement(10936..11007)
                     /locus_tag="ACL297_001724"
     tRNA            complement(10936..11007)
                     /locus_tag="ACL297_001724"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10973..10975),aa:Gly,seq:gcc)
     gene            complement(11030..11102)
                     /locus_tag="ACL297_001725"
     tRNA            complement(11030..11102)
                     /locus_tag="ACL297_001725"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11067..11069),aa:Thr,seq:tgt)
     gene            complement(11121..11202)
                     /locus_tag="ACL297_001726"
     tRNA            complement(11121..11202)
                     /locus_tag="ACL297_001726"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11166..11168),aa:Leu,seq:tag)
     gene            complement(11208..11280)
                     /locus_tag="ACL297_001727"
     tRNA            complement(11208..11280)
                     /locus_tag="ACL297_001727"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11245..11247),aa:Lys,seq:ttt)
     gene            complement(11305..11377)
                     /locus_tag="ACL297_001728"
     tRNA            complement(11305..11377)
                     /locus_tag="ACL297_001728"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11342..11344),aa:Asp,seq:gtc)
     gene            complement(11380..11452)
                     /locus_tag="ACL297_001729"
     tRNA            complement(11380..11452)
                     /locus_tag="ACL297_001729"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11417..11419),aa:Val,seq:tac)
     gene            complement(11457..11572)
                     /gene="rrf"
                     /locus_tag="ACL297_001730"
     rRNA            complement(11457..11572)
                     /gene="rrf"
                     /locus_tag="ACL297_001730"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(11649..>12365)
                     /locus_tag="ACL297_001731"
     rRNA            complement(11649..>12365)
                     /locus_tag="ACL297_001731"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3669 a         2934 c         2287 g         3475 t
ORIGIN      
        1 tgcaagccct atctacgata ctgtttcaag aaacgagtca tcttttcttg gatttgggct
       61 aactcatcaa cacgaggaag gtaaacgata cggaagtggt ctggttcctg ccagttaaag
      121 cctcgaccat gaaccaagag aaccttttcc tgcttcaaga aatcaaggac aaactgctca
      181 tcatcatcga tacggtacat attgcggtcg atttttggga agatatagag ccccgccttg
      241 ggtttaacgg cagacaaacc tggaatatct tgaatggcat tatagatgaa atttctttgc
      301 tcgtagattc gtccaccagg aagaagcaat tcatcgactg attggtgacc ccccaaggaa
      361 gtttgtacga cttgttgggc caaaacgtta gagcaaaggc gcatattgga cagcatattg
      421 agcccttcga tatagccctt aacatgagtc ttagggccag acaagaccat ccaacccaca
      481 cggaaacctg ctatgcggtg ggattttgac agaccattca tgctgacaca gaagacatct
      541 ggtgccaagc tcgccacagg cgtatgcaca tgtccgtcca ttaccatgcg gtcatagatt
      601 tcatccgcaa agataatcaa atcgttttga cgggcaatct caataatctc cagcaagagt
      661 tccttaggat aaagggctcc agttgggtta tttggattga taaggacaat tgccttggta
      721 ttggaagtga tttttgactt gatatcgtca atatctgggt accattccac agcttcatca
      781 cagatatagt gaacggcatt tcctccagct aggctgacag ccgccgtcca tagaggatag
      841 tctggcattg gcactaagac ctcatcccca ttgtccaaaa gcccctgcat ggacatgaca
      901 atcaactcac tgacaccatt tccaaggtaa atatcatcaa tatctacgtt tgggaatttc
      961 ttcagctggc aatactgcat gatggcctta cgggctgaga aaatcccttt ggagtcagaa
     1021 tagccttcac tatcacgcgc attcataatc aagtcatgaa tgacctcgtc tggcgctgta
     1081 aagccaaatt ctgctggatt tcctgtattc agacgtaaaa tcttttctcc attcgctcgc
     1141 atccgcatgg cttcttccaa tacaggacca cggatatcat aagcaacatg ctctaactta
     1201 ctagacttgt tatattcttt catcttgttt cctcaattca tcaggatagt aacattatac
     1261 cactagaaag agaatgcgac aagtttactg aaatgtgtta ctagtttcgt atagaagaaa
     1321 aaccttgcta aaggcgaggt taagaacgta gagatagcag gtattttgca aagacacatc
     1381 acataccaaa agataaacct agaaaaaagc aagcctagaa aggagtcata aagaagatta
     1441 aacatgagga aaagccccca tatcatcaag taatcccaac gcccacttcg ttttgcaact
     1501 agaaataaga ttagatggta gagtacatga aaaactaaaa atccaataaa acttggataa
     1561 agaagcggta ccagactctc taattgacag atcatgatca cttctaccaa gactgaaact
     1621 aggataattc cataataaag gaaatttgat ttttgaaaag agtttttcat cattctccct
     1681 tccttcactt ctttcctgct atcctttctt ttattttatc atatatttat atagagttta
     1741 agccctaata ttcttttgaa aaatactttt tttgctttac ttctatagtg aaaaggttta
     1801 caattatagt aagaaaattt caggaggttt cattatggca caacgttacc aaaatatcat
     1861 ggtcgcaatc gatggttcta aggaagcgga cttggctttt gtcaagggag ttcattctgc
     1921 tctacgaaac gacgctaaac tcaccatcgc acatgtcatt gacacacgcg ctctccaaag
     1981 cgtatccacc tttgatgctg aagtttacga agaactccaa gtcgacgctg aaagtctgat
     2041 gaaagagtac gaaaaacgtg ctaaagatgc tggggtagca gatgttcata tcgtcattga
     2101 aatgggaaat ccaaagaccc tgctagcacg tactattcca gatgccgagg aagtggacct
     2161 catcctcgtt ggcgcaactg gtctcaacgc ctttgaacgc ctcttggtcg gctcttcatc
     2221 tgaatacata ctccgccatg ctaaggtcga tttgctggtt gtgagagaac aagaaaaaac
     2281 cttataatca caaagaaaag gagcccctag ctcctttttg tttacgattt atttctctct
     2341 ttatggcgtt cgtaagcctt gagctggcgc tgcagttcct ttttaatagc aggttctgga
     2401 gcatattttt cttcccaatt atctggtttt aagattttat gggtcactgg atcaaaatga
     2461 gccttgccat ctggaaaaat tttccccata ttggcctgat ggacaatatc aaaaatacgt
     2521 tctgggtcca cccccatcaa gacaaaactg ccgtaggtga agtaaagcgt gtcaatcaag
     2581 gcatccactt gccctatcaa atcttgctga gcaggtgtct tcttggctac tttacctgct
     2641 gccttatcaa gggcctgatg aagttgcgat acagcttgac caaaatcttc ttcagaagga
     2701 ctggctgatc gaacaaactc caccaattct tctattttaa aaccagccct atgggttgca
     2761 ccctctaaat cccaagctcg aggttcttct tgggttcgtt catccatcat gtggtggaag
     2821 gtcttgacct tattgaaatg atagtcacgg ctgacaaaaa ctttttctga agacaaaaca
     2881 ccaaaatgtt ccaaggcctt gtggatccca tcttgttgat tgctagtggt aatgtgcttg
     2941 gcagcttcct tgacactgct actaccattt cccatagcga ccgacatacc gacgccagcc
     3001 agcatttcaa ggtcattatc tgagtcacca aaagccatga cttggttgag gtcaaagcca
     3061 tattctttcc caactcggcg aatgccttct aatttagaat ttccctgatt gatgacatcc
     3121 gatgcaaaag gattgctacg tgtcaatttc aagtcttcaa aatcagctgc cgccttctca
     3181 gattcttctg gtgtcatcag catcaaaact tggtaaatag gttgattcat caggtgaagc
     3241 aggtcctctt ccttttgggg aacaaccttg ctgaccatgc gattaaaaga ctgactcacc
     3301 gtccgagtta aaacagaggg aacgaagcga ctaattcgtt gggaaaaaga acccagacca
     3361 aaggacatga ttttagaacc caacatggca tccttggtcc ctaaggcaat ctccgtgccc
     3421 tcttttttag catagctaat tagatggcgc aaatgtaact tggaaatagg gctcgtgaac
     3481 aagactctgt ctttactaaa gatatactgg ccattatagg ttaccgcaaa atccagatcc
     3541 aaatcgtcca tcaattcctt aacaaaaaaa ggtcctcgcc ctgtcgctac gccaactagt
     3601 accccttgtt ctttgacaat cttaatcgca tccttagtgg atttcaaaac actcttgcga
     3661 ttgttgacca aggttccatc gatatcaaaa aaaacagctt tgacttccat cctatcccaa
     3721 tctccccttt tgtgatacaa tgattatacc acatttcaga aagagtgagt aaatcatgcc
     3781 taagaaaatc cttgttttac atacgggtgg aactatttcc atgcaggccg atgcttctgg
     3841 cgctgttgtg acgagttcag ataatcccat gaaccatgtg tccaacccac ttgaaggaat
     3901 ccaagtccac gccttggact tttttaacct tccaagtccc catatcaaac ccaaacatat
     3961 gctggtcctc taccagaaaa ttaaagagga agcagataac tacgatggag tggtgatcac
     4021 acacggaacc gatactttag aggaaacagc ctatttcctt gataccatgg aagttcccca
     4081 tatgcctatc gttctaacag gagccatgcg tagctccaat gaactcggta gtgatggtgt
     4141 ttataattac ctaagtgctt tacgagtggc cagcgatgac agggctgctg acaaaggagt
     4201 tttggtcgtt atgaacgatg aaatccacgc tgccaagtat gtcaccaaaa cacatacgac
     4261 taatgtcagc accttccaga ctccaacaca tggccccctt ggtctcatca tgaaacagga
     4321 aatcctctac ttcaaaacag ctgaacctcg tgttcgcttt gaccttgatc acatacaagg
     4381 tttagtccct atcatctcgg cttatgctgg tatgacagat gagctgattg atatgctgga
     4441 tttagaacac ttggacggtt tgattatcca agccttcgga gctggtaata ttcccaaaga
     4501 aacggctcaa aaattagaaa gccttctgca aaaaggaatt ccagtcgctc tggtatcacg
     4561 atgctttaac ggtattgccg agcctgttta tgcctaccag ggtgggggcg tacagttgca
     4621 aaaagcaggc gttttctttg ttaaagaact caacgcccaa aaagctcgct tgaaactcct
     4681 catcgccctc aatgccggac taacaggaca ggctttgaaa gactatatgg aaggctaata
     4741 ctcttcgaaa atctctgcaa accacgtcac gtcgccttac cgtatgtatg gttactgact
     4801 tcgtcagttt catctacaac ctcaaaacag tgtgttgcgc agcctttaac cagccgccta
     4861 gtccgctcta tggtattcat taagtcaaca tctcttgttt aagagcacaa aatcaggaaa
     4921 tcttctcgat tccctgattt tttctattta cgttttcgtg ttgagctacg ttctgtcaaa
     4981 ccatgaggta agagaacttc acgttcttcc aactcttcct tatgcataat cttggtcaac
     5041 atacgcatac taatggcacc aaggtcataa agaggttggg caatcgttgt caagtttgga
     5101 cgggtaaagc gtgagatttg tgaatcatca ctagtaataa tttcaaaatc ttctggcaca
     5161 gaaacaccct tatcagccaa accgttcaag actcctgctg ccaactcatc acctgtcaca
     5221 actgctgcag ttgcatttga tgaaatcaaa cgctctgcta aggcgtaacc atcatcatag
     5281 ctatatttag attcaaatac caaaccctca ctataagtga ttcctgcttt tttcaaggtt
     5341 tccttgtagc caactaaacg aaccttacca ttgatgtcat ccactagcgg accgctaacg
     5401 aaagcaatac gctcattttc tttaacaagg taactcactg catcaattgt tgcttgctta
     5461 tagtcaatat tgacacttgg caactggtgc tcaacatcga cagttcctgc gagaacaatc
     5521 ggagtacgtg aacgcgaaaa ttctgagcga attttatctg tcaagtgata acccatatag
     5581 ataatgccat ctacctgctt tgaaaagagg gtattgacaa cagaaacttc tttctcgtta
     5641 tcttcatcgc tattagctag gacaatattg tacttgtaca tttctgcaat atcatcaatc
     5701 cccttagcca aactcgaaaa ataaccattg gtaatatttg gaatcacgac accgacagtg
     5761 gttgtctttt tacttgcaag accacgcgca actgcatttg gacgataatc caaacgatca
     5821 attacctcta gcactttttt acgggtattc tcttttacat ttttattgcc attgaccaca
     5881 cggctgaccg tcgccatgga aacacctgct tcacgagcga catcataaat ggttactgta
     5941 tcatctgcat tcattccttt tcctgtcctt tctatctcac acattctttt acaagtagag
     6001 gtgctgattg aagctctata tctacttaca aaagtgaaga tgtgaaaatt tcgttttcat
     6061 atttctactt attccattct atcactaatt gtaaacactt tcaagtgttt tttgaagatt
     6121 gattgaaaaa atttcataga aaacctaggt ttagctcctt gctaccacct tagactaaac
     6181 aaaaaggagg aaactaagcc ctcctaaagt tatagtaaaa tgaaataaga acaggataaa
     6241 tcgatcagga cagtcaaatc gatttctaac aatgttttag aagtagaggt gtactattct
     6301 agtttcaatc tactataggt attgttccat tcactaccgt caattttagc acatagtctt
     6361 cataaaatat tatatcatca taaccaacca gattcttttg cgatattagc tgcctctgtt
     6421 cgattaccag catccagttt tgaaagaata ttggtgacat agtttcgaat agttccgttt
     6481 gagaggtaaa gctgatctgc gatttcttgg ttggataagc cccgggcgat tccctttaag
     6541 acagcaatct cttgttctgt caacggattg ggacgggtca tcaccacttc catcaattca
     6601 ggcgaatact ccttgcgtcc ttcgaggacg gtgtgcaagg tttgcatgag gtctgcaatg
     6661 cttcgttcct ttaatacata agcatctact ccagccttga ccgcacgttc aaaataccct
     6721 gggcgcttga aggtcgtcac tacaaccacc tttgtttcga gcttttctgc tcgtatccac
     6781 tccaagactt cgagtcctgt cttaacaggc atttctacgt caaggatggc gatatctaca
     6841 gactcctttt ctaatagttg aattgcttct tgaccattct tggcttgtaa gacagactct
     6901 acatctgctt gaaaggcaag caactggcac atggcatctc gcaacatact ttgatcttct
     6961 gcgactaata ctttcatcta ctttctctcc ttataaggta gtcgaacttg cacttccgtt
     7021 ggatgtttct gactgattac acttacttct cctgaaaatg gagaaacacg atctcggact
     7081 gtatggagct catctccttt tagaaaagca aagccgcagc catcatcact tactgttaaa
     7141 atgagttctt tctctgcccg ttctaatttc aggtaggcct tcgacgcttt ggcatgtttg
     7201 ataatattgg tcaccaactc aagcaaaatc atggaagccg ttgactccaa ttcttgagta
     7261 agactagcag tatctagttg gttatccgtt tccacctcaa ttccagcaat ttctaacatc
     7321 tttttcacag tctctagttc ggatgtcaaa gttctagact taagattttc cacaatggtt
     7381 cgcacttcat tcatggaatc cttactgatc tggtgaattt cttttaattc cttttccacc
     7441 tgtggataag cctccatctg aaataactgc aaggctaaat ctgtcttgac actcaacata
     7501 gcaaaggtat gtcccaagct atcatgcaaa tcctgaccga tacgactgcg ttcattttca
     7561 gcaagcaata gatttatctg ggcattttgc ttgacctgag cttctttcaa atcctccaca
     7621 atacgaatcc gaaccaagcc aaaagtcatt aaatcgacaa aagcaagaat tacaagtaga
     7681 tagaatagaa actcaacttc gattctctga aaaatcaaca gttggcccac aacaaggact
     7741 tgagcaagaa gaaaagtcca gacatgtaaa gactttaaac cacctacgct gaaatgataa
     7801 cttaagagat tggataggaa aaagaaaaac cagatataat taacagcaac aaaggcagta
     7861 ttcccaacta cataagtcag catgaggccc caatatagcc aagataggcg ctggctctta
     7921 gttgttaaaa cacccaaata cgccactaca aatagaatat caatcaataa atgccagcta
     7981 ggaatctccc cagtcactac aggtaggatg gggaaaatca taaaaattaa actggcccaa
     8041 tacatatagt ctatgctttt tagtctttca agcattaagc attctcctta tgaccttgaa
     8101 gataaatggt caaaccaaac aaaactgctg aaaaaacaag tatataaact gtggctgata
     8161 gattgatgcc accctcgttt aagaaggttt tgagcaactc catcaactga taagttggta
     8221 ggcacttccc gattgcttgc atccagtctg gaaataaaga gatgggcatc cagagtccgc
     8281 ctaaaacagc caagcctaga tagagaagat tgcccacgac agacatcaac tgactagaag
     8341 gcaagagtgt caaggtcaag ccaagcgcta caaaggcaac acttcctact attagcaaga
     8401 acgcagcccc aatccagctt cctagaggca tatccacacc tcttacaaaa tgcccaactg
     8461 agaaaacaac cagaattgat accaaataat caaccatcat acttgttatc tttgatagat
     8521 aatattctac catatttaca ggggtatggc gcaatgtttt ctgccagttg ttgattttat
     8581 cagtatgtaa gacagctggg aatgaaaaca tagctgtcga catcatggaa aaggctgtca
     8641 tggagatgag gtaatcacgc ataaaattcg cgagttcacc tggtgtgtcc tgatagatac
     8701 cagaaaaaaa taaatagaaa gccgtcggca tccctaccga caatagataa tagattaatt
     8761 gtcgtttggt caataaaaat tctatcttac taagtgctat ccatcgtttc atcttagtta
     8821 tctccctttt gtgtttcttc aaagattgta ttcaacaaac tacgattatt aacttcgatt
     8881 tcttgcatcc tacatcctgc ttgggctaat agttcccaga aagcatctgc ttctcgagtg
     8941 actacttgta gagcatcctg tttttgtgac cagttttcaa ccaagttaga ctgctcaatg
     9001 acttccttgt atgccagagg aaggataaaa tgcttttcaa ttccctcact acgcatagct
     9061 agaggcgtcg tatcacgaat caactctccc ttatttaaaa ccaaaatccg gtcagccgta
     9121 tgctctacct cttcaatata atgagacgaa tagagaatcg tgactccttg cgcttttagg
     9181 tcccgaacga tttcccaaaa gcgttgacga gttgaagtat ccatggcagc agttggttca
     9241 tctaaaaaga caagctttgg tcgcccaatc aaggtcaaga caaaagagaa gagacgcttt
     9301 tgcccgcctg acaatttttc tgcgaattgc tctttttgtt gctggtcaaa ctgcaatagt
     9361 tgatcgattt cctgatcgct caaggaattt ggatagatac gttgaaagaa agcaatcaac
     9421 tctttgacct ttaatttctg aacgatgaca ttttcttgag gcagataacc tctaatatag
     9481 tctaactgag aactcgtcac tgacaagcct tggatggata cttgaccgct tgtgaccagt
     9541 ttatctccaa gcagacagtc caagagtgtg gtcttcccag caccattggg cccaatcaag
     9601 gcgacgcatt caccttcagc tacctcaaag gaaataccct tcaaaatagc cttgcccttg
     9661 atgtttttat ttaggctttc taccttaatc atattcatga tattctcctt tcaaccactc
     9721 cattctcata aggaaaacga cgaaaatcat aaatccaaac cccaaagcac cacgaatgaa
     9781 ttggcgaagc aaggtttggt caaaccaacc tgtaaacatt tccactaacc ataccaagag
     9841 tgacaggccg ataaagaaat agatgatccc tctcttcatt cctcaaactc ctttttcaca
     9901 tctccgacta atttcaaacc ttctctaaca agccaagaca tcattccaaa gccagcaaag
     9961 agctcccaag gaaaatgata gaaactctca tccaatcccg aaaacatgag ttaggtcata
    10021 actcctgcta ctactaaact cactgcgata atcattttat ttctcatctc ttcttcctcc
    10081 atttcatact acaattatag tcttttgaaa tcagaggaga cagaagcttc tgtcactaga
    10141 aaatatgaca aatgtcataa aaaattctgt tcaaaacaag caagatacac tatacaataa
    10201 aacacaatca gaaaaatcta aggcaacttc ctcaaaagag atatcaaacc caattcacac
    10261 cataatgtaa actaatactt atttaaaatc aaaaagagta gaaattttta tcagacaaac
    10321 acatatatag tgtattgaat ctataacagt agggcttaaa tactaacata tttctataaa
    10381 ttaatttaac tttcctgata gagctgttca tatcttattt caattctcta tattatacgt
    10441 tgaacaaaac ccttctattg ctttcttaaa aatttataag agttataaaa tctgttaaat
    10501 ttcaatgtgt atacctaaac tacggtattt attgaaaaga ctggggacaa aaagtatacg
    10561 ctgccaaaat gaattactga aaatcaaaaa agagagaacc aaactgattc cctcttaatg
    10621 tatataatat ctagttttaa aaatacacac tcacatatct ctgtaatgaa tcgggaagac
    10681 aggattcgaa cctgcgacac cttggtccca aaccaagtac tctaccaagc tgagctactt
    10741 cccgagttaa atagaaaaat gcaccctaga ggagtcgaac ctctaaccgc ctgattcgta
    10801 gtcaggtact ctatccagtt gagctaaggg tgctccatat tatgccgagg accggaatcg
    10861 aaccggtacg atcgttacca atcgcaggat tttaagtcct gtgcgtctgc cagttccgcc
    10921 accccggcct ctctaagcga acgacgggat tcgaacccgc gacccccacc ttggcaaggt
    10981 ggtgttctac cactgaacta cgttcgcact gttttcttct atctaaaaat gccggctaca
    11041 tgacttgaac acgcgaccct ctgattacaa atcagatgct ctaccaactg agctaagccg
    11101 gctcatttgt tatatcttaa tgcgggttaa gggacttgaa cccccacgcc gttaagcgcc
    11161 agatcctaaa tctggtgcgt ctgccaattc cgccaaaccc gcatatatga cccgtactgg
    11221 gctcgaacca gtgacccatt gattaaaagt caattgctct accaactgag ctaacgagtc
    11281 taaaataact tgcgttatct taaacggtcc cgacgggaat cgaacccgcg atcttcgccg
    11341 tgacagggcg acgtgataac cgctacacta cgggaccttt gggagttaac gggatcgaac
    11401 cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaaactc cctaaagcta
    11461 agcgacttcc ctatctcaca gggggcaacc cccaactact tccggcgttc tagggcttaa
    11521 cttctgtgtt cggcatgggt acaggtgtat ctcctaggct atcgtcactt aactctgagt
    11581 aatacctact caaaattgaa tatctattca acttaagaaa accgttcgct ttcatattct
    11641 cagttacttt ggataagtcc tcgagctatt agtattagtc cgctacatgt gtcgccacac
    11701 ttccacttct aacctatcta cctgatcatc tctcagggct cttactgata tataatcatg
    11761 ggaaatctca tcttgaggtg ggtttcacac ttagatgctt tcagcgttta tcccttccct
    11821 acatagctac ccagcgatgc ctttggcaag acaactggta caccagcggt aagtccactc
    11881 tggtcctctc gtactaggag cagatcctct caaatttcct acgcccgcga cggataggga
    11941 ccgaactgtc tcacgacgtt ctgaacccag ctcgcgtgcc gctttaatgg gcgaacagcc
    12001 caacccttgg gaccgactac agccccagga tgcgacgagc cgacatcgag gtgccaaacc
    12061 tccccgtcga tgtgaactct tgggggagat aagcctgtta tccccagggt agcttttatc
    12121 cgttgagcga tggcccttcc atacggaacc accggatcac taagcccgac tttcgtccct
    12181 gctcgagttg tagctctcgc agtcaagctc ccttatacct ttacactctg cgaatgattt
    12241 ccaaccattc tgagggaacc tttgggcgcc tccgttacct tttaggaggc gaccgcccca
    12301 gtcaaactgc ccgtcagaca ctgtctccga tagggatcac ctatctgggt tagagtggcc
    12361 ataac
//
LOCUS       DBEJAQ010000052        12049 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.052, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000052 DBEJAQ010000000
VERSION     DBEJAQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.052"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(92..1294)
                     /gene="rpsA"
                     /locus_tag="ACL297_001732"
     CDS             complement(92..1294)
                     /gene="rpsA"
                     /locus_tag="ACL297_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGR0071947.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1456..1527)
                     /locus_tag="ACL297_001733"
     tRNA            complement(1456..1527)
                     /locus_tag="ACL297_001733"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1493..1495),aa:Gln,seq:ttg)
     gene            complement(1537..1617)
                     /locus_tag="ACL297_001734"
     tRNA            complement(1537..1617)
                     /locus_tag="ACL297_001734"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1581..1583),aa:Tyr,seq:gta)
     gene            complement(1667..1897)
                     /locus_tag="ACL297_001735"
     CDS             complement(1667..1897)
                     /locus_tag="ACL297_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HGR0071948.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2065..2709)
                     /gene="pcp"
                     /locus_tag="ACL297_001736"
     CDS             complement(2065..2709)
                     /gene="pcp"
                     /locus_tag="ACL297_001736"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGR0071949.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2724..3647)
                     /locus_tag="ACL297_001737"
     CDS             complement(2724..3647)
                     /locus_tag="ACL297_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0859 family membrane protein"
                     /protein_id="HGR0071950.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMSNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3644..4333)
                     /locus_tag="ACL297_001738"
     CDS             complement(3644..4333)
                     /locus_tag="ACL297_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0858 family membrane protein"
                     /protein_id="HGR0071951.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4398..5102)
                     /locus_tag="ACL297_001739"
     CDS             complement(4398..5102)
                     /locus_tag="ACL297_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGR0071952.1"
                     /translation="MVLSTSAILVACGKTDKEADEPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(5158..6180)
                     /locus_tag="ACL297_001740"
     CDS             complement(5158..6180)
                     /locus_tag="ACL297_001740"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HGR0071953.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6312..8783)
                     /gene="parC"
                     /locus_tag="ACL297_001741"
     CDS             complement(6312..8783)
                     /gene="parC"
                     /locus_tag="ACL297_001741"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGR0071954.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(8984..9188)
                     /locus_tag="ACL297_001742"
                     /pseudo
     CDS             complement(8984..9188)
                     /locus_tag="ACL297_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(9203..11146)
                     /gene="parE"
                     /locus_tag="ACL297_001743"
     CDS             complement(9203..11146)
                     /gene="parE"
                     /locus_tag="ACL297_001743"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGR0071955.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGFKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAVVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11282..11923
                     /gene="plsY"
                     /locus_tag="ACL297_001744"
     CDS             11282..11923
                     /gene="plsY"
                     /locus_tag="ACL297_001744"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGR0071956.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(11966..>12049)
                     /locus_tag="ACL297_001745"
     CDS             complement(11966..>12049)
                     /locus_tag="ACL297_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGR0071957.1"
                     /translation="IDYYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT         3472 a         2735 c         2111 g         3731 t
ORIGIN      
        1 accttagctg acttcaaccc actacagttg acaaagagcc agaaaaatcc cttgtttagt
       61 aaacaaagga ttttgtgaat tttcaatttg attaaagttc gatatcacca aacaaatcag
      121 ccattgaaaa tcctgtttgt gtttctggaa gttcgaaatc acgcttttct tgacgtcttg
      181 gacgacgtgg acgagcagca cgtttttctt ctttttgtcc ttcttcttgg gctggacgtt
      241 cttcaagagc tttaatagaa agtgatacgc gttctgcatc tgcgttaact tcaagaactt
      301 taacttgaac ttcttgacca actttaagag cttcttttgg attttcaatc cgtttgtgtg
      361 aaatttgtga tacgtgaaca agtccatcga tacctggcaa tacttcaaca aatgcaccga
      421 agtcagtcaa acgtttaact gttccttcta ctacatcacc tttagccaat ttttgctcaa
      481 cgccatccca tggtcctggt actgttgctt taagtgaaag tgatacacgt ccttcttctt
      541 cgttaagatc aaggattttc acttcaattt cttcaccaac agttacaact gattttggtg
      601 atacgttacg ttcatgtgac aattcagtca agtgaaccaa tccgtcaaca ccaccaaggt
      661 cgatgaaagc gccgaagctt gtgatacgag caactttacc agttacaaca tcaccaacag
      721 ccaatttacc gaatacttca gcgcgagctg ctgcagtagc tgcttcaaca acttcacgac
      781 gtgaaaggat gaagcggttt tctttagcgt taacttcttt gattttagta tcaaattctt
      841 gacctacaaa acgctcagcg ttacgtacga aacgagtatc caacattgaa gctgggataa
      901 atccacgaac accttcaaat tctactgaaa gtccaccttt aacggcacgc gttcctttaa
      961 cagtaacaac ttcttcttcg cgaccaacaa gtttgtccca tgctttgcga gcttcaaggc
     1021 gttttttaga tacaaggtat gtaactgtat cagtatcttt accaactact tgacgaagta
     1081 caagaacatc caatacttct cctactttaa caaagtcatt gatatctgca tcacgatcgt
     1141 ttgtcaattc gcgaagagtc aagacacctt caacaccagt tccagagatt gcaacgttag
     1201 cttgagtcgc atcaactgtc aatacttcag cactaacaac atcaccagtc tcaacttgac
     1261 taacgctatt tagcaaatct tcaaattcgt tcatctaaaa aatcctccaa caatcaagtt
     1321 tttcttaaac ttgacatact tataattttt ttcctaagca cccgcaagga catgttcata
     1381 tcattcacgt ctttcaatta taaaaataaa ataccctaag atattttgct ttttatcttg
     1441 attattacct aagaactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
     1501 cttgccttac cgcttggcta taccccatta atgaaatgga gagagaggga ttcgaacccc
     1561 cgaacccgaa ggagcggatt tacagtccgc cgcgtttagc ctcttcgcta tctctcctac
     1621 aatcaacatg gactattata tcatgaaaat ttcaaaaaaa caagacttat tttgctaaat
     1681 tataattttc aatcaaaatt tccacagctt tttccaattt tttataaaaa ctatcgacat
     1741 tcccattctt tataatggca aattgtccgt ctaattcggc aatttctccg ataacttttt
     1801 taccgatagt caatgtataa ccttcaaaac tgtctttacc aacattaact ttggcatccg
     1861 ctacttgaat ttcgattttt ttatctttct tgctcatttc atacctctct tcactttttc
     1921 tattttacaa gaaaatccta aaactagcaa gaaaaaactc tatatcaggc ttctctgata
     1981 tagagtttta tgtaaacaac ctcaatcgat ttagcatagc taatagttag taaacaagca
     2041 taattgacta tcctttttag gttatcagtg agtctcaccg cctactaact gcaaatcctt
     2101 atctttgtag tctacaatag cttcaatagc tacctctatg cctcttacaa tatcacatag
     2161 actcattcct gcagtattcg gcttatttac tacttgttcc atcatatatg gaatatgcat
     2221 gaaacctgct ctcatattag ggaatttttt atccactaaa taaagagctt ggtacatcaa
     2281 atgattgcaa acaaaggttc ctgcactatt ggatacaact gccggaagtc cctgtttttt
     2341 gatagcttgt accatcgctt tgataggtaa actactaaaa taggccgatg ctccatcaat
     2401 acgaatcggt gtatcaattg gttgattgcc ttcgttatca ggtatgcgag catcatcttg
     2461 attaatagcc actcgttcag gtgttaagcc ggtcctgccg cctgcttgtc caatacaaag
     2521 tacagcatct ggttgatatc gtaatatttc tgcctctaaa acttctgacg acttataaaa
     2581 aaccgttgga atttctaccc agcgaacttc agccccatta atctcagatg gtaataattt
     2641 tacagcctcc aaagctggat taatcttttc acctccaaaa ggattaaaac ctgtaaccaa
     2701 tattttcatt ttattttcct ttactaaaat gcgagaaagt acattaagaa tatgtgaata
     2761 acaatcatta ctagagcaac acctgcttga gcctttataa cgccattctg atctttcata
     2821 tccatcaatg ctgctggtag agcgttaaaa ttagcagcca ttggggtcaa taaggtccca
     2881 caataacctg ctgtcatggc aagagcacca gccacaattg gattagctcc cagagcaaat
     2941 acaaagggaa ctccaacacc tgctgtaata acggtgaatg ctgcaaaagc atttcccata
     3001 atcattgtga atagaaccat tccaagaaca taggccaaaa ctcctataaa gcgactatct
     3061 gaaggaacaa taccgctaat cagatgagag ataacatcac caacacctgc tacagtaaaa
     3121 atagccccca aagcccctaa taattgagga acaataccac ttgttgaaac ttgctgagtc
     3181 attcgattat tttctgataa cagactctta gggtgactat tggtaatcac aagaacagaa
     3241 attgtagcaa acaaggcggc aaggctaatc gaaatcttgc taaattctgg aatcatttgc
     3301 gctaagacca acgcaagtat tgccatcagc ataactggaa taaaaatttt atttttcaac
     3361 ctgttagatt caatattggc tttcatttca tctaaggatg gcaaggttcc gatacggact
     3421 tgcttaaaca atgttaacag cgataatagg attacaataa taccaatact catatttgac
     3481 atataggaac cacctataaa cgtaatagac aatagagtcc aaaatgcaga tgtcccaagt
     3541 cgaactgggt ttgttttatc tttataacta caataggctg tatggagaaa ttgacaacca
     3601 atcacaatat aggtcaactc taatagttgc tttgccaact ctgtcatttt tgttctcctc
     3661 ccctagtctt ttttgatatc aattttttat caaataaata attgtaaatc cccactacaa
     3721 taagtgttat aacagcaaca ataatagatg tagaagcaat ccctgcataa ttgctttcat
     3781 agcctaactg atctaatgtt ccccctatca agaggactcc cccagcacct acaaacgtat
     3841 tttgagcaaa gaaatttcca aaattttcat tcgcagccgc acgcgctttt attgtctcat
     3901 cttcaacctc tgttaacttt ctacctaatt gagactctgc agctgcttct cccataggtt
     3961 gaaccaaagg tctgacaaac tgagggtgtc ctcctagacg aattgaaaag aaaccagcta
     4021 actctcgaat aaaaaaataa actgtataga agtttccaac tgtcagacct ttaatctttc
     4081 gaatcaaatc gattgatcgt tgcttgagtc caaaggtttc tgacagcccc acaagaggca
     4141 aggtaaccat aaaaatcgtg agcactcgct gattgctaaa ttcttttccc aaaatctcca
     4201 aaaattcaac gagagaaaca cctgaaacta aagctgtaac caaaccagct aagactactg
     4261 ttgcaattgt atcaaatttt aaaataaaac ccacaacaat gattgctatt cctattaatc
     4321 taatccactc catatcaaac tcctttatat tcaaaatgac agtattttaa aaattttatc
     4381 aagatcaata ccattcctta tttaatgtat ttttctagtt ctttttggta tttgctattg
     4441 gattccaatt tttctttttg ccatttttta aaaacctcgt tatattcttt tgttgtaaca
     4501 atatcttttt gcaatttcat tcctttaaag atatatggtt cccccttaat accaacttgt
     4561 gagtatggtt ttgagaatgg tactacgtta cttacaactg gagaaccacc agatgaagct
     4621 gttggcatca ataatgaact atctgtcgac caagcttgag ctttggcata tttttcatat
     4681 ctcttctcta ggtcagtggt ctcagaaaca gcatcttcta acaatttctt atatttatcc
     4741 aaaccaggtt tagctacaac atccttatct tttcctttcg taataccaag gtgtttcatg
     4801 gcagaaccag attttggatc cataatattc aagtaagacg ctggatcttg atagcttgga
     4861 gcccatcctg tactattcaa atcatagtct ttttgagaag gagcaacatt gccgtattta
     4921 tcattttcca tcaaaccatc aataacattt ccaataacgt ctgtcctcga tgttcgagtc
     4981 gctatactgt agcccaatga tgctggatct actgcataga cataagaaaa tgttgtcggt
     5041 tcatctgctt ctttatcagt ttttccacaa gccactaaaa tagctgacgt gctcaggacc
     5101 actcctgctg ttaagagcca ctttttctat ttcataaaaa atctcctttg gtttatttta
     5161 atctactttt acaatccaac cttctggcgc ttcaatatcg ccaaactgaa tacccgtcaa
     5221 ttcattatat aatttacgcg tcacaggacc tacttctgtt tcactataga atacatggaa
     5281 atcatcacca tgttgaatac ctccaattgg agaaataacc gctgctgtac cacaggcacc
     5341 tgcctctaca aaacggtcaa gattatcaat tggaacatca ccctcaatag gagttaatcc
     5401 caagcgatgt tctgccaaat aaagcaagga atacttggta atagatggca agatagatgg
     5461 actcaatggt gttacaaatt cattatcagc tgtaattcca aagaagttag ctgatccgac
     5521 ttcttcaatc tttgtatgag ttgatgggtc cagatagata acatctgaga aatgacgtga
     5581 cttggccatt tttcctggta agagacttgc agcatagttt ccaccaacct tagccgcacc
     5641 tgtaccattt ggtgctgcac ggtcgtactc atcctgaatc aagaagttgg ttgggaccaa
     5701 accaccttta aagtaatttc caactggcat agcaaagatg gtgaaaatgt actcttctgc
     5761 cggttttacc ccgataatat ctccgacacc aatcaaaaga gggcgaagat ataaggttcc
     5821 acctgttccg tatggtggta cgtattcttc attcgcacgg acaactgctt tacaagcttc
     5881 tacaaacatg tctgtcggaa cttgtggcat caagagacgg tcacatgtac gttgcagacg
     5941 tttagcattt tcatcaggac ggaacagttg aacactgcca tccttagtac gataagcttt
     6001 caaaccttca aatgcttgtt gtccatagtg aagacttgga gaagactctg aaatatgcaa
     6061 agttgcatcc tctgtaagct ctccttgatc ccattgtcca tttttgaaat gagcaagata
     6121 gcgataaggt aatttcatat aggaaaaacc gaggttttcc caatcaatcg ttactgtcat
     6181 attccactcc ttattctaaa aacctatttc ttatattcta cactattttt ctaaaatagc
     6241 aagtatattt tgtaattttc agaaaatttc tccaataaaa accagctctt agaactgatt
     6301 cttcatttca cttatttatc ttcagtaact acttcctgaa gataagcgtc aaaaacttct
     6361 tcatctgaaa tcgtgtcaga aatgaagctt ccattgctag tgcgttctga caagttcaag
     6421 tcttgcaatc ggctttcata gattgttcct ttattggatt ggacaagcag agtttggtcg
     6481 ttcacatcca cttccgtact gaagaaatcg ccaacaaatc cttgctctgc aactgctcct
     6541 gccaagaaga cacgatgcgg tttgtttttc aactcacgca agacttgtaa tcctcgtttg
     6601 gcacggctgg ttgctagaat ttcctcaatg gaaacacgtt tcaagcttcc acgctgggtc
     6661 aagaggtaga aggacgaagt attacagata aagccagatt ggaggacatc atcttctttc
     6721 aaattcatag ccttgacacc tgctgcctta gcaccgacaa ccggaacctc ttcgatattg
     6781 aaacgcaggg cataaccatt ttgactaacc aagacaacat catctagttt aatcggagcc
     6841 actgctacaa tctgatctgt atcgtctttg agcttagcat acttgacaga cttagatcta
     6901 taggtccgcc atggagtgaa ttcttttcgc tctacccgtt tgatttgacc aaggcgagtc
     6961 actgcaaagt aggttgtcgc atcgtcaaac tgatccagta cttccacata aaggatttct
     7021 tcattcgttt caaagtttgt gatggtttgg ctcagatgct ctccgatgtc cttccaacga
     7081 atatctgcca actcatggat tggtctgtag atgacatttc caagacttgt gaacatcaag
     7141 aggtgctggg ttgtcttggc agattgaaca aaaatcaaac ggtcatcatc acgcttgcca
     7201 atttcttcca aggtggaagc cgcaaaggaa cgtggactgg tacgcttgat gtaacctgcc
     7261 ttggtcacgc tgacgtaggt atcttcctca gcgataagac tagctgtatc aatctcaatt
     7321 gctttcgcag tgtcttctaa agaactcaaa cgaggagttg caaatttctt cttgacctca
     7381 cgaagttctt tcttcatgag attgtacata gtcctttcat caccgataat agccgccagc
     7441 atagcaatct tctcacgaag ctctgcttct tcttcctgca agacaaccac atcggtattg
     7501 gtcaaacggt acagttgcaa agttacgata gcctcagcct gttcttccgt aaaatcatag
     7561 ctaactttga ggttttcctt ggcgtccgcc ttattctcag aagcacggat aagagcaatg
     7621 acttcatcca aaatcgaaat cacacgaatc aaaccttcga cgatatggag acgtttctca
     7681 gccttttctt tgtcaaagcg tgaacgcgcc aaaatcactt ctcgacggtg agcgatgtag
     7741 ctagacagga ttggaacaat cccaacctga cgaggtgtga aattgtcaat cgccaccata
     7801 ttaaagttgt agttgatttg taggtcggtg tacttaaata agtagttgag aacaagctca
     7861 gtattagcgt ctttcttaag ttcgatagcg atacgaagac catcacggtc agactcatca
     7921 cgaacctcag caatcccagc taccttgtta ttaacacgaa catcatcgat tttcttgact
     7981 agattggcct tattgatttc ataaggaatc tcagtaataa cgatttgttc cttaccacct
     8041 tttagctttt caatttcagt cttggaacga acaaccacgc gccctttccc agtttcataa
     8101 gctttcttga tttcatcacg accctgaata atagcccctg tagggaagtc tggtccaggc
     8161 aagaattcca tgagtttatc aatctttgca gttgggtggt caatcatgta aactgcagca
     8221 tctatgacct cagctaaatt atggggagga atgtctgtgg cataaccagc cgaaatccca
     8281 gtcgaaccat tgaccaagag gtttggaaag gctgctggca agaccgttgg ttctttctcc
     8341 gtatcgtcaa agttccatgc aaaaggaact gtatttttct cgatatcctg aagaaggtaa
     8401 cctgcaattt cagacaaacg tgcctcagta taacgcatag ccgcaggagg atctccgtcc
     8461 atagaaccgt tattaccgtg catttcaact agaatctcac gatttttcca gttctgtgac
     8521 atacgaacca tggcatcata gatagaagaa tccccgtgtg ggtggaaatt ccccatgatg
     8581 ttcccgactg acttggccga cttacggtag ctcttgtcaa aagtattgct atccttattc
     8641 atagaataaa gaatacggcg ctgaaccggc ttcaacccat cacgaatatc tggcaaagcc
     8701 cggtcttgaa taatgtactt ggagtagcga ccaaagcgct ctcccatgat gtcctccagg
     8761 gacatgtttt gaatgttaga cataagatac aaagcccata aaataccaag tgaaaataga
     8821 aaattcttga agtaagcaaa ctcacaagag aatttatctt tttcacacag tatctagggc
     8881 gtgttcaact cctttcaaag aatgtagagt agttttttat gcagtaaaag atattttacg
     8941 ggaattcctc ccgtgttcag ttacgataag taaccaaact atcctgtttg tatttttcaa
     9001 tatgaaaatc tggttttcca aaattagtct tagtttgtgt cttagccgct cccttaagcg
     9061 cctctttgag ataagcactc atagcagatt cttcattaat aatcctgcaa ttttttcaaa
     9121 ccaagatttt caaactgctt tttcacatag tcattcacat ccgactctaa tttccagttt
     9181 actaacatat tattttcttt cattaaaaca ctgtcgcttc ttctagcgta aacttgacat
     9241 tatcttcaat ccatttacgg cgtggttcta ccttatctcc catgagaaca ttgacgcggc
     9301 gttcggcgcg cgctaaatct tcaattgtga cacggatgag ggtacgtgtt tctgggttca
     9361 tggttgtttc ccagagctgg tccgcattca tctcaccaag tcctttgtat cgttggaggg
     9421 tagcgccttt accgaactgt ttacggagtt cttctagttc tccgtccgtc caagcgtagg
     9481 ccacttcttc tttcttgcct ttacctttgg acatcttata aagaggtggg agggcaatat
     9541 agacatgacc tgcctcgact agcggacgca tgtaacggta gaaaaatgtc aagagcaagg
     9601 tctggatatg ggcaccgtcg gtatccgcat cggtcatgat aatgatctta tcatagttgg
     9661 catcttcaat agagaagtct gctccaacac ccgcaccaat ggtataaatc atggtattaa
     9721 tctcttcatt tttgaggata tccgccatct tggccttggc tgtattgaca accttaccac
     9781 gaagaggtag aatagcctgg aacttgcggt cacgaccttg tttggcagaa ccaccggcag
     9841 agtccccctc aactagatag agttcattct tagcaggatt cttagattgg gctggggtca
     9901 atttcccaga caacaagccc ttatctttct tgtttttctt cccatttcgg ctctcatcac
     9961 gcgccttacg tgctgcttca cgagcatcac gggccttgat agccttgcgg atgaggttag
    10021 aagctaattc cccattttcc ataaggaaaa aggtcaactt atcagccact attccatcca
    10081 caactgggcg agctaggggg cttcctagtt tatccttggt ctgtccttca aactgcaagt
    10141 gttcttcagg aactaagata gaaagaacga ccgctagtcc ctcacgatag tctgaacctt
    10201 caaggttttt atctttttcc ttgagaagac ctgttttacg tgcatagtca ttcatgacct
    10261 tggtaatggc agacttgaat cctgtctcgt gcgttccacc gtccttggtg cgaacgttat
    10321 tgacaaagga tagaatgtta tctgagaatc cgtcattgta ctggagggct acttccactt
    10381 gaaaaccatt gtcttcccct tcaaagtaaa gaactggcgt caagatttcc ttatcttcgt
    10441 tgagataaga aacaaaatct tgtactccat tctcatagtg gaactcaatc gcttcatctg
    10501 ttcgcttgtc cgttaaagac aaggtcacat ttttcaagag aaaggctgat tcattaaggc
    10561 gctctgaaat ggtattgtac ttgaaatctg tcgtagaaaa gatagtcgcg tcaggcataa
    10621 aagtaacttt ggtgcctgtt ttagacttgg gtgctgtacc gattttcttc aaagtcgtga
    10681 caggttttcc accattttca aaacgttgct tgtaaactgc gccatcacgg gtaatttcaa
    10741 cttctaacca gctagaaagg gcgttaacaa cggaagaacc cactccgtga agtccacctg
    10801 atgtcttata gccaccttga ccgaatttcc ctccggcatg aagaatggta aagataacct
    10861 caacagttgg aattcccata gcgtgcatac ctgtcggcat cccacgtcca tggtcttgaa
    10921 ccgttagact accgtcttta ttgatagtta catcaatacg atcaccaaac ccagacaagg
    10981 cttcatcgac tgcattatca acgatttccc aaactaggtg atgaagacca gcgccatcgg
    11041 tcgatccaat atacatccct ggacgttttc ggaccgcatc caacccttct agcacctgaa
    11101 tagcatcatc attataattg ttaatattga tttccttttt tgacacaagg aacctcctat
    11161 tcgttcatct ttactattct acaggttttc caaggatttt gcaaaatttt tctttctccg
    11221 atgtgacaat ttcagcagag attctctgct tttctttccc aattcatgat ataataggag
    11281 tatgattaca atagttttat taatcctagc ctatctgctg ggttcgattc catctggtct
    11341 ctggattgga caagtattct ttcaaatcaa tctacgcgag catggttctg gtaacactgg
    11401 aacgaccaac accttccgca ttttaggtaa gaaagctggt atggcaacct ttgtgattga
    11461 ctttttcaaa ggaaccctag caacgctgct tccgattatt tttcatctac aaggcgtttc
    11521 tcctctcatc tttggacttt tggctgttat cggccatacc ttccctatct ttgcaggatt
    11581 taaaggtggt aaggctgtcg caaccagtgc tggagtgatt ttcggatttg cgcctatctt
    11641 ctgtctctac cttgcgatta tcttctttgg agctctctat cttggcagta tgatttcact
    11701 gtctagtgtc acagcatcga tcgcggctgt tatcggggtt ctgctctttc cactttttgg
    11761 ttttatcctg agtaactatg actctctctt catcgctatt atcttagcac ttgctagttt
    11821 gattatcatt cgtcataagg acaatatagc tcgtatcaaa aataaaactg aaaatttggt
    11881 cccttgggga ttgaacctaa cccatcaaga tcctaaaaaa taaaacgcca gttctgtact
    11941 gaccccaaaa agttagacaa ttaatttatc cgaaggattt agttctgtat tgcacagggc
    12001 taagtccttt tagttttacc ttaattcgtt tattgttgta gtaatcaat
//
LOCUS       DBEJAQ010000053        12048 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.053, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000053 DBEJAQ010000000
VERSION     DBEJAQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.053"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            94..1095
                     /locus_tag="ACL297_001746"
     CDS             94..1095
                     /locus_tag="ACL297_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGR0071958.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    1307..1536
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1702..1953
                     /locus_tag="ACL297_001747"
     CDS             1702..1953
                     /locus_tag="ACL297_001747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071959.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSSKYCFEVVDRTDEVS
                     SKHCFEVADRTDEVSNHTYGKATLTRFEEIFEEYKGVSK"
     gene            2326..3687
                     /gene="trpE"
                     /locus_tag="ACL297_001748"
     CDS             2326..3687
                     /gene="trpE"
                     /locus_tag="ACL297_001748"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGR0071960.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNSFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGVTDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3684..4250
                     /locus_tag="ACL297_001749"
     CDS             3684..4250
                     /locus_tag="ACL297_001749"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGR0071961.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4261..5265
                     /gene="trpD"
                     /locus_tag="ACL297_001750"
     CDS             4261..5265
                     /gene="trpD"
                     /locus_tag="ACL297_001750"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HGR0071962.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDVRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5262..6029
                     /gene="trpC"
                     /locus_tag="ACL297_001751"
     CDS             5262..6029
                     /gene="trpC"
                     /locus_tag="ACL297_001751"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HGR0071963.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLNYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            6016..6615
                     /locus_tag="ACL297_001752"
     CDS             6016..6615
                     /locus_tag="ACL297_001752"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HGR0071964.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6593..7816
                     /gene="trpB"
                     /locus_tag="ACL297_001753"
     CDS             6593..7816
                     /gene="trpB"
                     /locus_tag="ACL297_001753"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGR0071965.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7809..8585
                     /gene="trpA"
                     /locus_tag="ACL297_001754"
     CDS             7809..8585
                     /gene="trpA"
                     /locus_tag="ACL297_001754"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGR0071966.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8557..8658)
                     /locus_tag="ACL297_001755"
                     /pseudo
     CDS             complement(8557..8658)
                     /locus_tag="ACL297_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            8887..9618
                     /locus_tag="ACL297_001756"
     CDS             8887..9618
                     /locus_tag="ACL297_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071967.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            9633..10784
                     /locus_tag="ACL297_001757"
     CDS             9633..10784
                     /locus_tag="ACL297_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0071968.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(10836..11495)
                     /locus_tag="ACL297_001758"
     CDS             complement(10836..11495)
                     /locus_tag="ACL297_001758"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGR0071969.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWKLLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTCHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            11565..12011
                     /locus_tag="ACL297_001759"
     CDS             11565..12011
                     /locus_tag="ACL297_001759"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGR0071970.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE"
BASE COUNT         3820 a         2102 c         2755 g         3371 t
ORIGIN      
        1 atttgtggat cttaaataat ttactaaatt ctcttgttaa aaaaaattaa atattgtata
       61 gtaatattag aatgttcgta atggaggact gaaatgaaaa atataaatgg gaaaaaggta
      121 actatttatg atattgcacg tttatcaggt ttttctccaa aaacagtttc acgagttatt
      181 aatggtggag taaatgttaa agaagaaact tatcaagcga ttcaaaaagt tatagaggaa
      241 ttgtcgtata ttccaaatgc gtatgcaaaa aatttaacta aaaaagaagc tataaatata
      301 ttgatttctg taaaaaaaat cgactctttt cctttaattt ggtttcatac gctgttagat
      361 aaagtgttac ggacgtgtaa agaatttggt gttaatgcta tagttgagta ctttggagag
      421 gaggatacaa ttagtaattc aattatatca agcacaggta gcctagtaga tggtgttatt
      481 gttttttatg aaagtgtaga tgatatcaga attcagtatt taaagaagaa ccatatgcct
      541 tttcttgttt tcggtgaatc tcaaacgtct ggagtagtct atgtatctaa taataatttt
      601 caagctactt atgatatgat gaaagctgta actgaagaaa aatttaaaaa tatgtggttg
      661 cttatgggag gagaatccca tgttaataag gatcgtgaaa gaggtgtgcg ttcttttctg
      721 aatgataaaa attactttat ggatttaaaa gtcatttatg gcttatctac aattgattca
      781 gtttattctt atgcaatgca gcacttgact acccaaaatt atccggacat tatttttgtt
      841 tcaggagatg aaaaggttca gggcttgatt cgtgcgtgct atgagaaagg aattcttatt
      901 ccagatgata tttctatcat tggatttgat aatattccta tttctcagta ttatactcca
      961 gctttgtcta caattgctcc caattatgtt aaattggcta aagaaatgat agaaggtgtt
     1021 ttagcaatta ttaaagggga aagtgtcaca tctgttgaag tttctcctaa atttgttagg
     1081 agacagagtt tctagtattg tttaattctt gaaaatccta gtggaaaaga tggagtgttc
     1141 taaaataagt tctgtttagc ctccattcag tttctattga gttggatcca atttctaaca
     1201 agtgggacat gttcatgttc gatctaagga agacatggtc acattcgaat gatattttca
     1261 aaaaaatcgt aaaaaagtct tgacaaagaa aaaacaaagt attaaaataa gttcaacaaa
     1321 tcagaaaagt aactattttt gatcttcagg gagcctgtgg tggttgtgaa taggtggttg
     1381 gaagtagtga aagtgggctg attttgaaaa tgaatttgaa acaatgaaaa ttcggtgcgc
     1441 acaccttaca gtgcagcttg ttgttaaaca aggcagagat gtaaagggat agtcccttta
     1501 taattgaggt ggcaccgcgt taccaacgcc ctcacacgga agtatatttt gtgtgtgggc
     1561 ttttttctat ccgtcgtttg gtttatcttt tattagggcc ttaagtcgct ttgatgaact
     1621 tgagttctat ctacagctcc ttgcctacta ctaaaagcaa acaaaaagct ggactaatac
     1681 gaaaaaagct agtaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt
     1741 tgctcaaaac actgttttga ggttgtggat agaactgacg aagtcagctc aaaatactgt
     1801 tttgaggttg tggatagaac tgacgaagtc agctcaaaac actgttttga ggttgcagat
     1861 agaactgacg aagtcagtaa ccatacctac ggcaaggcga cgttgacgcg gtttgaagag
     1921 attttcgaag agtataaagg tgtatcgaaa taaaaatctc ctcatcaata tctctggtcg
     1981 tggaggcaag gacctagctg cgattgcaga ttacctagaa gctaaaaaat aatagatgga
     2041 aaaattacag tcttttctct cacagagagc ttgtggttgc tgaaaacaag cagagaaagc
     2101 tgtaaagttg ggttcatctg aaacgagaga agaaaacatc attctcaagg gagtgccctt
     2161 tatcgcacag cctgttacag gaggtatctg agacaggaat gagagatagg agaaatccta
     2221 taattgaggt ggcaccgcga atttcgtcct cacgcaagtt attttgcgtg gggatttttc
     2281 atacagtcac cacggactag aagtagaact gaaagggaaa ttacaatgga acgaatcatt
     2341 catggagatg tcttatcacc aatcttggct tatatgcgct taaaggggca acacaaggtt
     2401 atcttagaga gtattccgag agacaaggaa acagctcgtt tttctatctt agcctataat
     2461 cctgtttttg agattaagtt tgaaaatgga gttctttatc aaaatggtca agtgattgat
     2521 cgagatccct tggatttcct ttatgaagtg attcataaga gtcagcacca ctcagagcta
     2581 ccttttggtg gtggagccat tggttttgtg ggttacgata tgatttcgct ttatgaagaa
     2641 attggtcaaa tccctgagga tacaattggg acgccagaca tgcatttctt tgtctatgag
     2701 agctatatgg tctttgacca caagaaggag aagatacatg tcatagagga tgctctctat
     2761 agtgagcgca gtcaagaggc cttggaaaaa tccttgaacc aagtgcttga ggaattacgc
     2821 attcctgctc caaatgaatt tgaagacttg gatttatcac cacttgactt caaaccgcat
     2881 atcgctccac ataagtttga ggtaatggta gaaacagctc gtgacttgat tcgtaatggt
     2941 gatatgttcc aatgtgtact cagccagcgt ttctcagcag aagttactgg aaattcattt
     3001 gacttctaca gaaatctccg cgtgaccaat ccatccaatt acctctattt ctatgatttt
     3061 ggggattatc aaatcatcgg agctagtcca gaaagtttag tttctgtcaa aaatggcatc
     3121 gtgacaacca atccgattgc agggacgcga ccaagagggg ttacggatga agaagacaag
     3181 accttggcga ctgacctcct ctctgatgag aaggaaacag cagaacatcg aatgttggta
     3241 gacttggggc gtaacgatat tggccgcatc tctgaaacgg ccagtgtcca agttactaag
     3301 tatatgaagg tggagctctt ccgctacgtc atgcatttga ccagcgtggt caaggggcgt
     3361 ttgcttgcag aactcactgc catggatgcc ttgaaagcaa cgcttcctgc tggaaccgtt
     3421 tctggagcac caaagattcg ggcgatgaga cgcatctatg aactggaaac ggaaaaacgg
     3481 ggcgtatatg caggagcaat cggctacttg tctgcgacgg gtgatatgga tttggccatt
     3541 gccatccgaa ccatgattct caaaaatcaa agagcctatg tgcaggctgg ggcagggatt
     3601 gtctacgact ctatcgccca aaacgaatac caagaaacca ttaacaaagc aaaatctatg
     3661 actagaattg gagaactaag accatgattt tattgattga caactatgat tcttttacct
     3721 ataacttggc ccaatacatt gggaattttg cagaagttca ggttctgaga aatgatgatt
     3781 ccaagctgta tgaagaagct gaaaaagcag atggtctggt cttttctcct ggtcctggtt
     3841 ggccagttga tgctggaaag atggaagaca tgattcgtga ttttgcaggc aagaagccga
     3901 ttctagggat ttgtttgggc caccaagcca ttgcagaagt ctttggtggt aagctaggtt
     3961 tggctccaaa agtcatgcat gggaaacaga gcaatatcaa ctttgaagcg ccatctgttt
     4021 tgtatcaagg tattgaggat ggacgtgcgg tcatgcgtta tcacagtatt ttgattgagg
     4081 aaatgccaga agactttgaa gtgacagctc gttcgactga tgaccaagcc atcatgggga
     4141 ttcaacataa aaacctaccg atttatggct tccagtacca tccagagagc attggaacgc
     4201 cagacggctt gtcttctatt cggaatttta tcgagaaggt tgtaaagtga ggaaactagg
     4261 atgaaagaga ttattgaaaa actagcaaaa tttgaaaatt tatcaggtgt ggaaatgacg
     4321 gatgtcattg agcgtatcgt aactgggcgt gtaactgaag cgcagattgc ttctctcctc
     4381 ttagctctta agatgaaggg ggaaacacct gaagaacgca cagccattgc ccaagtcatg
     4441 agaggacatg cccagcatat tccaactgag attcatgatg ccatggacaa ctgtggtaca
     4501 ggtggggaca agtctttcag ttttaatatt tccacaactg cagcctttgt cttggctggt
     4561 ggcggtattc acatggccaa gcatggtaac cgctcgattt cttctaaatc tggttccgca
     4621 gatgtcctcg aagccttggg aatcaatctt gacctcaaac cagctgaact aggtaaggtc
     4681 tttgataaaa ctggaatcgt ctttctcttc gctaaaaata tgcacccagc tatgaaatac
     4741 atcatgccag ctcgtttgga attgggaatt ccaacgatta tgaacttgac tggtccactg
     4801 attcatccaa tggctttaga aacacagctt cttggaatta gtcgtccaga actcctagaa
     4861 agtacagctc aggttttgaa aaatatgggt cgcaaacgtg ccatcgtggt tgctggacca
     4921 gaagggttgg atgaagctgg cttgaacgga acaaccaaga ttgcacttct tgaaaatggc
     4981 gaaatcagct tgtcaagctt tactccagag gatttgggaa tggaaggcta tgctatggaa
     5041 gatgttcgtg gtgggaatgc tcaggaaaat gcagaaattt tgcttagcgt tctgaaaaac
     5101 gaagcaagtc cattcttgga aacgacagtc ttgaatgctg gtcttggttt ctatgctaat
     5161 ggtaagattg atagcatcaa ggaaggagtt gccttggccc gtcaagtgat tgctagaggc
     5221 aaggcccttg aaaaactcag actgttacag gagtatcaaa aatgagtcag gaatttttag
     5281 cacgaatctt agagcagaag gcgcgtgagg tggagcaaat gaagctggag caaatccagc
     5341 ctctgcgcca gacctatcgc ttggcagaat ttttgaagaa tcatcaggac cgcttgcagg
     5401 taatcgctga ggtcaagaaa gctagcccta gtttgggaga tatcaatctc gatgtggata
     5461 ttgtgcaaca ggcccagact tatgaagaaa acggagcagt gatgatttcg gtgttgacag
     5521 atgaggtttt ctttaaaggg catttgaatt atctacggga aatttccagt caggtagaga
     5581 ttccgacgct caacaaagac tttatcatag atgaaaagca aatcatccgc gctcgcaatg
     5641 caggtgcgac agttatcttg cttattgtgg cagccttgtc cgaagaacgc ctcaaggaac
     5701 tgtatgacta cgcgacagag cttggtctgg aagtcttagt ggagactcac aatctagctg
     5761 aactagaggt agcccacaga cttggtgctg agattatcgg ggtcaacaac cgcaacttga
     5821 ctacctttga ggtcgacttg cagaccagtg tagatttagc cccttacttt gaggaaggtc
     5881 gctattacat ttctgaatct gccattttca cagggcagga tgcggaacga ctagccccat
     5941 actttaacgg aattttggta gggacagctc ttatgcaggc agaaaatgtg gcccagagaa
     6001 tcaaggagtt gcagattgac aaaggttaaa atttgtggac tatcgaccaa agaagcggtg
     6061 gaaacagccg tttcagcagg agccgactat atcggttttg tctttgcacc tagtaaaaga
     6121 caggtgactt tagaagaggc agctgagttg gcaaagctta ttcctgcaga tgtgaaaaag
     6181 gttggagtat ttgtttcacc aagtcgggta gaactgctgg aagcgattga caaagttggc
     6241 ttggacttgg ttcaagttca cggtcaggtg gcagctgatt tatttgagaa tttgccttgt
     6301 gccagtattc aggctgtgca ggtagatgga aatgggcatg tccccaattc tcaggcagat
     6361 tatctactct ttgatgcccc tgtggcagga agtggtcagt cctttgattg gggtcaactg
     6421 gatacgactg gactagcaca gcccttcttt atcgcaggtg gccttaatga agataatgta
     6481 gtaaaagcaa ttcaacattt tactccctat gcagtagatg tatcgagcgg agtggagaca
     6541 gatggacaaa aagatcatga aaagattaga agatttatag agagggtaaa gcatggcata
     6601 tcaggaacca aataaagatg gattttacgg aaaattcggc ggacgttttg tcccagaaac
     6661 attgatgaca gcagttttgg agttggagaa ggcctaccgt gaaagtcagg cagacccaag
     6721 tttccaagag gaattaaacc agcttttgcg ccagtacgta ggacgtgaaa ctcctcttta
     6781 ttacgcaaaa aacttgaccc agcatatcgg cggagccaag atttatctca aacgggaaga
     6841 ccttaaccat acaggagccc acaagattaa caatgccttg ggacaagttt ggcttgccaa
     6901 acgcatgggt aaaaagaaaa ttatcgcaga aactggtgct ggtcagcacg gtgtggcaac
     6961 tgcaactgct gcggccctct ttaacatgga atgtaccatc tacatgggtg aggaagatgt
     7021 caaacgccaa gccctcaatg tcttccgtat ggagcttttg ggagctaagg tcgaggccgt
     7081 gacagatggt tcgcgcgtgc tcaaggatgc ggtcaatgca gcccttcgtt catgggtggc
     7141 taatatagat gatacccact atatccttgg ttctgccttg gggcctcatc catttccaga
     7201 aattgttcgt gacttccaaa gtgtcattgg tcgagaggct aaacaacagt accgtgactt
     7261 gacaggacaa aatctgccag atgccctagt agcctgtgtt ggtggtgggt cgaatgccat
     7321 cggtctcttc catccctttg tagaagatga gtcagtagcc atgtatggag ctgaagcggc
     7381 tggatttggt gtggatacgg agcaccacgc agctaccttg accaagggtc gtccaggtgt
     7441 ccttcacggt tccctcatgg atgtgctcca agatgcccat ggtcaaattc ttgaagcctt
     7501 ctctatctca gcaggtttgg actatcctgg tatcggtcca gagcattctc actaccacga
     7561 tattaaacgt gccagctatg tccctgtgac agacgaagaa gccttggaag gattccaact
     7621 cttgtctcgt gtggaaggga ttatcccagc tttggaatct agccatgcta tcgcctttgc
     7681 ggtgaaattg gccaaagaac ttggaccaga aaagtctatg attgtatgcc tatcaggtcg
     7741 tggggacaag gatgtggttc aagtaaaaga ccgcttggaa gcagatgcag caaagaaggg
     7801 agaagctcat gcctaagaca ctaacaaaaa aattaaatgc tattaaagca gctggaaaag
     7861 gaatttttgt tccctatatc atggctggtg accacgaaaa aggtttggat ggtcttgctg
     7921 aaacaattca ctttttagaa gatttgggtg tctctgcaat tgaagtgggt attccctttt
     7981 cagaccctgt tgcagatgga cctgttatcg aagaagctgg cttgcgcagt ttagcccacg
     8041 ggacctctac ccaggcttta gttgaaacct tgaaaaccat tgaaacagag attccactgg
     8101 tcatcatgac ctacttcaac cccctctttc agtacggtgt ggagaacttt gtcaaagatc
     8161 tggctgatac agctgtgaag ggcttgatta tcccagacct gcctcatgaa catgccaact
     8221 ttgtagaacc atttttggca gatacagata tcgccttgat tcctttagta agcttgacca
     8281 caggaattga gcgccagaaa gagttgattg aaggggcgga gggattcgtc tatgccgttg
     8341 ccatcaatgg ggtaacaggg aaatcaggca attaccgtgc agatttggac aagcacttgg
     8401 cgcaacttca tcaagtggcc gacatcccag tcttgacagg ttttggtgta tctagtcaag
     8461 ccgatctgga acgcttcaat gcggtgtcag atggtgttat cgtcggttcg aaaatagtaa
     8521 aagctctcca ccaaggagag ccgattcagg actttatcaa acaagcagta gcttaccaaa
     8581 aataattaaa caagtagcaa gtaagaggaa aggcacgaaa ggaagtcttt cctttttctt
     8641 ttgcaggaga aaggctagtt ttatagtgaa ttgaatctgg aatattacac tacggttcct
     8701 aaaatatttt aagaaagtga tttgaatttc ccaataaatt tgttcagttt ttaattgatt
     8761 ttactataga agatataatt ttctaaatta ctctgacaca gaactttaag aaaaagctaa
     8821 ttattgtagt aatttcttta agaaaatgat ataattcatt aaaaagtatt tggaggagga
     8881 agaattatgt tgaatcagga tctctttgat tcgcttgagg cacaaaaaat tgtagatact
     8941 ttgatgaaag ggcaaaaaga ttatgtagat gaacgtctag aaaaaagaga gacaatgata
     9001 gtgtcgaatg gttatgcatg gacgcgacct aatcatattg atactgcatt tgcatcagca
     9061 gatttgtttg agtataaatt acaattagca ggacagactt ggggatattt agaatttgaa
     9121 acaaatacag aaaaatatgg gaaagtattg ttaattataa agggtaagaa gcgacttacg
     9181 aaccaatttc ctttggtaca aaaaaataag agtggctact tatttgaata tgctcaaatg
     9241 aatacacttt atcttaatca acattcttcc tacaaaaatg atgaagatag tcattccttt
     9301 ccaattcaga tggagttagt ttctgatgaa atgattcaag aaattgaaca agctaccaaa
     9361 aattcgaata tcgaaaaatt tatgatttta acttatgagg cggactcaga aaacaatatt
     9421 atatctgtag atgttgttat gcctgatgca cgaactggtc agttacactt gattcaagat
     9481 ttgtctgagt atattcaatc aagttcgtac catttcgaag aagctaaata ccaagatatt
     9541 cctaattttt cagaattatc ggaaacagaa gattttgaaa ttattccaag aatagaaaaa
     9601 caagaaggtc aaaagtagta aggagtatgt aaatgtttaa tggtcgggta ttgaaagaat
     9661 tacggctgtt aaatggttta agtagagcag aattagctca gagaattaat ttaacggaac
     9721 aagccatttg gcagtttgag tccaacgaaa cgaaacctaa attatcaacc aaaatgcatt
     9781 tggccaacca atttcatgtt gatttaactt attttgaaca ggaagaagag agcattcgat
     9841 ttgattcttc tgtaattgcc tttagaaatg cagacctagc aacacggaaa acaatagata
     9901 ttcaaactat gtatttacat aaggtagata gtttgattga ttattttgaa agttttgtaa
     9961 ttatacctaa tattacaatt catgacctaa gtaatgtagt gagtgaatct tatcataagg
    10021 gagaatccat tgaggaattg gctctttatg ccagggaaaa attaggtatt tcaaaagata
    10081 atcatgattt gctttataaa ttagaacgtt caggcatcta tatcgtggaa cgattaatta
    10141 atggccaagc tgatgcttat agcgcatggt caaaattggg aagaccttat attgtgttgg
    10201 gaacgaataa atcatctgta cgtcgaaatt ttgacttagc tcatgagcta ggacatattc
    10261 ttttacataa atataaagat atgaatgaag atggcgatcg tttggagcaa gaagcaaatt
    10321 attttgcatc atgtttttta ttgccaaaag aagagttttt agtcaaattt gaagagaggg
    10381 ttggcaagcg tgtcagcaat cctgatagtt atattttatt gaagtcggat ttgaatgttt
    10441 cgatacaggc tttagagtat cgagctttta agttaggatt attgactcca aagcaacatt
    10501 cttactttta tcgtcaaatt gcgcaaaaag gttacaaaat gattgaacct ttggatgatc
    10561 aaatttttgt taaaaaacca agcaaagtaa agagtattct ggacgtcgtt ttgagtaatc
    10621 atctagtcag tctagcgact ataatgtcta aacaaagtat tcgtttacag tttataagcg
    10681 aaatattttc agtcgaaatg aaattttttg atcagtatca agaagataga agaacagatc
    10741 gatttgataa catcatccct ttgtacaaaa gaaataattt ataatttaac tgtgaaataa
    10801 tttcatgaag gacatatggc agaaattgga ttttattaga caagcagtag cttaccaaaa
    10861 ataatcaaac aagtagcaag taagaggaaa ggcacgaaag gaagtctttc ctttttcttt
    10921 tgcaggagaa aggccaggat acccgtcgca gaagcgaact gaatcaagat cagtaactcc
    10981 gttacgctaa agacgagagc acaagaagct aaaaaaagga aatcccctgc acccatgcgg
    11041 atatcgataa aatgagccaa aattccaagt atgaggaagg agaccatgac cagattccag
    11101 ccagaggaag ctattaggat taggtggcaa gtcatccaga ccagtaaggg atattcctga
    11161 tggtgaaagt cgtagatacc caaggtcaaa ccagcggtga ttaggacgac ttgccccaag
    11221 gaaagcaatt tccaagagta aagcagaaag aggagtccta agactaattc aaagagggca
    11281 taccagacag gatagcgaac tttgcagtag cgacagcgaa agcgattgaa gacctgtgag
    11341 agaatcggaa tcaaatctaa gggacgcaag cgagtctgac aggaatcgca gtgactggct
    11401 gaactgataa tggattgctc tggaaaacgg tcaatgacca aaccaagaaa ggaagcgaga
    11461 atgctcccga cgagaaaaaa ataaaaatca atcatactta tctattcgta aaaaatggga
    11521 gaaatagtat aatggagaaa catagataag atggtgagga aaagatgaca attcgttttg
    11581 aagaaaaggt gagtatagaa aacgctcaat ttgtatgcca gtggtccaac tccctaggca
    11641 aatcctttca agaacaatgg atgggaccaa ggattccttt tctactgacc cttcaagcct
    11701 tggaaggagt cttttcaatc tttgatgaac aagagtttgt gggatttatc cagaaaatca
    11761 ggctagaaga cagcaatctt catatcggga gattttttat caacccccag aaacaggagc
    11821 aaggcttagg tagccaggct ttaaggaaat ttgttagttt ggcctttgaa aatgaagata
    11881 tagatagtat ttctctaaat gtcttcgagg caaatcaaag agctcagaat ctttaccaaa
    11941 aagaaggatt tgaaatcgtt caaatggttg aagcacctgt acgaaaatat agtatattga
    12001 aactagaata gtacacctct acttctaaaa cattgttaga aatcgatt
//
LOCUS       DBEJAQ010000054        11777 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.054, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000054 DBEJAQ010000000
VERSION     DBEJAQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.054"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            225..2516
                     /gene="pcrA"
                     /locus_tag="ACL297_001760"
     CDS             225..2516
                     /gene="pcrA"
                     /locus_tag="ACL297_001760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HGR0071971.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2559..3239
                     /gene="radC"
                     /locus_tag="ACL297_001761"
     CDS             2559..3239
                     /gene="radC"
                     /locus_tag="ACL297_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGR0071972.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3236..3925)
                     /locus_tag="ACL297_001762"
     CDS             complement(3236..3925)
                     /locus_tag="ACL297_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGR0071973.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3943..4584)
                     /locus_tag="ACL297_001763"
     CDS             complement(3943..4584)
                     /locus_tag="ACL297_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGR0071974.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4667..4882)
                     /locus_tag="ACL297_001764"
     CDS             complement(4667..4882)
                     /locus_tag="ACL297_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGR0071975.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4890..5237)
                     /locus_tag="ACL297_001765"
     CDS             complement(4890..5237)
                     /locus_tag="ACL297_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HGR0071976.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5242..6357)
                     /locus_tag="ACL297_001766"
     CDS             complement(5242..6357)
                     /locus_tag="ACL297_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGR0071977.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6367..7326)
                     /locus_tag="ACL297_001767"
     CDS             complement(6367..7326)
                     /locus_tag="ACL297_001767"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGR0071978.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7439..8008)
                     /locus_tag="ACL297_001768"
     CDS             complement(7439..8008)
                     /locus_tag="ACL297_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HGR0071979.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8136..8807
                     /locus_tag="ACL297_001769"
     CDS             8136..8807
                     /locus_tag="ACL297_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGR0071980.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8791..9609
                     /locus_tag="ACL297_001770"
     CDS             8791..9609
                     /locus_tag="ACL297_001770"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HGR0071981.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9606..10502
                     /locus_tag="ACL297_001771"
     CDS             9606..10502
                     /locus_tag="ACL297_001771"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGR0071982.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10546..11520
                     /gene="pta"
                     /locus_tag="ACL297_001772"
     CDS             10546..11520
                     /gene="pta"
                     /locus_tag="ACL297_001772"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGR0071983.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3423 a         2259 c         2542 g         3553 t
ORIGIN      
        1 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ttgaggtatt
       61 gagctagttt atgaaaaaat tgggtgaaaa gtcgagtgtt ttagaaaccc acagtgtagt
      121 attctagttt caatccacta tattttgcta ctccccgtaa agtttctatt ttccctgatt
      181 tctgatataa tagaaatatt gacttcaaga gtaaggaaga gaagatgaac gcattattaa
      241 atggaatgaa tgaccgtcag gctgaggcgg tgcaaacgac agaaggtccc ttgctaatca
      301 tggcaggggc tggttctgga aagactcgtg ttttaaccca ccgtatcgct tatttgattg
      361 atgaaaagct ggtcaatcct tggaatatct tggccattac ctttaccaac aaggctgcgc
      421 gtgagatgaa agagcgtgct tatagcctca atccagctac tcaggactgt ctgattgcga
      481 ccttccactc catgtgtgtg cgtattttgc gtcgcgatgc ggaccatatt ggctacaatc
      541 gtaattttac aattgtggat cctggtgaac agcgaacgct catgaaacgt attctcaaac
      601 agttgaactt ggatcctaaa aaatggaatg aacgaactat tttggggacc atttccaatg
      661 ctaagaataa tttgattgat gatgttgctt atgctgccca agctggcgat atgtatacgc
      721 aaattgtggc ccagtgttat acagcctatc aaaaagaact tcgtcagtct gaatccgttg
      781 actttgatga tttgattatg ctgaccttgc gtctctttga tcaaaatcct gatgttttga
      841 cctactacca gcaaaaattc caatacatcc acgttgatga gtaccaagat accaaccacg
      901 ctcagtacca attggtcaaa ctcttggctt cccgttttaa aaatatctgt gtggttgggg
      961 atgcggacca gtctatctac ggttggcgtg gtgctgatat gcagaatatc ttggactttg
     1021 aaaaggatta ccccaaagcc aaggttgtct tgctggagga aaattaccgc tcaaccaaaa
     1081 ccattctcca agcggccaac gaggttatta aaaataataa aaatcgccgt cctaaaaatc
     1141 tctggactca aaacgctgat ggggagcaaa tcgtttacta tcgtgccgat gatgagctgg
     1201 atgaggctgt atttgtagcc agaaccatcg atgaacttag tcgcagtcaa aacttccttc
     1261 ataaggattt tgcagttctc tatcggacta atgcccagtc ccgtacaatt gaggaagccc
     1321 tgctcaagtc taacattcct tataccatgg ttggcggaac caaattctac agccgtaagg
     1381 aaattcgcga tattattgct tatctcaacc ttattgctaa tttgagtgac aatattagtt
     1441 ttgagcgtat tatcaacgag cctaaacgtg gaattggtct aggtacagtt gagaaaatcc
     1501 gtgattttgc aaatttgcaa aatatgtcta tgctggatgc ttctgctaat attatgttgt
     1561 ctggtatcaa gggtaaggca gcccaatcta tctgggattt tgccaatatg atgcttgatt
     1621 tgcgggagca gctagaccac ttaagcatta cagagttggt tgagtccgtc ctagaaaaaa
     1681 caggttatgt cgatattctt aacgcccaag cgactctaga aagcaaggca cgggttgaaa
     1741 atatcgaaga gtttctttct gttacgaaga actttgatga caccacggat gtgacagaag
     1801 aggaaactgg tctggacaaa ctgagtcgtt tcttaaatga cttggctttg attgccgaca
     1861 cagattcagg tagtcaggag acatcagaag tgaccttgat gaccctgcat gctgccaaag
     1921 gtctcgaatt tccagttgtc tttttgattg ggatggaaga aaatgtcttt ccacttagtc
     1981 gtgcgactga agatccagat gaattagaag aagagcgccg tctagcctat gtaggtatca
     2041 cgcgtgcaga gaaaattctc tatctgacca atgccaactc acgcttgctt tttggtcgta
     2101 ccaattataa ccgtccgact cgttttatta acgaaatcag ttcagacttg cttgagtatc
     2161 aaggtctggc tcgtcctgca aatacaagct ttaaggcatc atatagcagt ggtagtattt
     2221 cctttggtca aggtatgagt ttggctcagg ctcttcaaga ccgtaaacgc ggtgctgccc
     2281 caaaatcaat ccagtcaagc ggtcttccat ttggtcaatt tacagctggc gcaaaaccag
     2341 catctagcga ggcaaattgg tccattggtg atattgctct ccacaagaaa tggggagagg
     2401 gaaccgttct ggaagtttca ggtagcggtg ctaggcagga attgaaaatc aatttcccag
     2461 aagtaggttt gaaaaaactt ttagccagtg tggctccaat tgagaaaaaa atctaatttt
     2521 ccatccttct cacgaataat aaagtgagga ggatttttat gtacagtatt tcattccaag
     2581 aagattcact attaccaaga gaaaggctgg ccaaggaagg agttgaagcg cttagtaacc
     2641 aagagttgct agctatttta ctcaggacag gaacacgtca agttagcgtt tttgaaattg
     2701 cccaaaaagt cttgaacaat ctttcaagcc taacggattt gaaaaaaatg accctgcagg
     2761 aattgcagag tttgtctggt attgggcgtg ttaaggccat agaattacaa gctatgattg
     2821 aactggggca tcgtattcac aaacacgaga ctcttgaaat ggaaagtatt ctcagcagtc
     2881 aaaagttggc caagaagatg cagcaggaat taggggataa aaaacaagag cacctggtgg
     2941 cactctatct caatactcaa aatcaaatca tccatcagca gaccattttt atcgggtctg
     3001 taactcgtag tatcgctgaa ccgcgagaga ttcttcacta tgcaatcaag catatggcga
     3061 cttctcttat cttggtccac aatcatcctt caggagcggt agcgcctagc caaaatgatg
     3121 atcatgtcac taaacttgtt aaagaagcct gcgaattgat ggggattgtt ctcttggacc
     3181 atttgattgt ctctcattct aattacttta gttatcgtga aaagacagat ttaatctaaa
     3241 gttcattaac gacatagtca aagagttttt tatctttggg acgattttca aaaagaagtt
     3301 ctggatgcca ttggacaccg agaaaggcga catcatccgt actcatgaca gcctcaatga
     3361 taccatcttt aggatcatga gccacaactt ttaaatttgg tgctaagtcc ttgatgctct
     3421 ggtggtggaa ggagttgata tgagagattt ctccatagat ttcttggaga acggtatctg
     3481 gttctgttac caagcgttga gttgtgtact caacagaaga atcctgccaa tggtcttcga
     3541 tatcttggta caaagttcca cccatggcaa cgttaaagag ttgggtacca cggcagacag
     3601 agaaaatggg ctttttctgt ttaatagctt ccttgatgag ggtcagttcg aagatatctc
     3661 tttgaaggtg atagtcatcg ctatcaatgg ttttgggttc gccataaaat tttggatcga
     3721 cattttgccc acctgtcaag atgagcttgt caatcaaact gatatagtgg caggccattt
     3781 cttgatcacc aatcggtagg atgatgggaa tccctccagc atctttaacg ccttcaacaa
     3841 agccttttgc tgcgtagctc atcatgatgt catcatctgg atgagctttt tcgtttcctg
     3901 taatcccaat aactggtttt ttcataaaat gattttcgct ttctaatcct cttttcgcat
     3961 gaagtagagg agggtttgga gttcacttgt caaatcgaca tactgaacga ccacgtcttt
     4021 tggtaaatgc agatggactg gtgaaaaact gagaattcct ttcacaccag catcaaccaa
     4081 gagattagca acctcttgtg acttgacgct gggaacagtt aggatagcag tcttcacatc
     4141 agcatccttg attttatcct tgatctgaga aatcccgtaa atgggaatcc cgtcaggagt
     4201 ttgggtaccg acttcaggat ggtcgtctag gtcaaaggcc atgataatct tcatcttgtt
     4261 acgttcgtgg aagcggtagt ggagaagggc atggcccata tttccaatac caaccagcat
     4321 gacattggta atagagttgt cattgagcaa atcggcaaaa aatgtcatta gttttttgac
     4381 atcatagcca aaaccacgac gaccaagttc accaaaatag gaaaaatcac gacgtacggt
     4441 cgctgaatca ataccgatag cctctgcaat ttgcttagag ttggcacgtt caatcttttc
     4501 tgcatgaaat ctcttaaaaa ttcgatagta gagagagagt ctttttgctg tagcttttgg
     4561 aatagcaaac tgtttatctt tcacaaaatc acaacctttc tattcttcta ttttatagaa
     4621 acattgtgaa aaaatcaaca aaaataagaa aaaactaaga aaaatcttag ttttgatgta
     4681 aaaaatctgc atgagataga aaacggtaga ggtctccgac cagaccctga taaacttttt
     4741 ggcccctaaa agtcagagaa gtcacataaa gtgtatctgg taaggttaca catcctgaca
     4801 aagtcaacat gagagcctca tgatcctcat acttgagagt acgctctaca tgatagcagt
     4861 ccttataggt cagttcaaac attttggctc tatctttccg attttgtaaa gacaccacgt
     4921 tctaccaagc tatccatgag gaagtagaat ttttcctgat gaatatggtg gtcttctgat
     4981 ttgaaaatat caactagacg aaggccaaac ttgtcagtga tattgatttt agcccctgta
     5041 agttccttgt taatgatgat tttgagttgg aagccttcac cgctgtttgg cactttttcc
     5101 aaaaggcgag tcagttcata gttaccaacc ttagtttcaa aaaaggtgtt atctttgagg
     5161 gtgaattttt taacagaagg gctaagagtg taatcgtaac gacaattttt taactgaatg
     5221 attttttcaa atgccatatg gctaacctcc gataatttct tttaaggttt ttgcgagggt
     5281 ttgtaggtct tcaacggtat tttgtggcga caaactgatg cgaagggatt ccttcaagcg
     5341 ttctgaattt gcgccataca tggcttcaag aacatggctg gattggacaa cgcctgcagt
     5401 acaggctgag ccagtagaga ttgaaattcc agctaaatct agccgaagga gtaagaggtc
     5461 atttttctga ccaggaaatc caatattgag aacataaggg agatgatgtt ttcctctatt
     5521 caagtaatac tgaatgccct ccagctctgc cagaaaggca gtttctagat tttgtacatg
     5581 ttgaaaatgt tcttcttgtt tttctaggtc ttcttttagg gctgcaacca tgcctacgat
     5641 agcaggcaga ttttcagttc ctgcacgttt tttctgttcc tggtctccgc catgtagata
     5701 ggaatcaaag tccatgctag atgcgtagag aaaaccgatt cccttaggac catggaattt
     5761 gtgggcagaa gcagtgagaa aatcaatgcc caattcttct gaatgaattg ggattttacc
     5821 aatagcctga actgcatcaa catgataggc agcagggtgt tgcttgagta tttggccaat
     5881 ttcagcgatg ggcagtaggt ttcctgtctc attattggca aacatggtag aaaccaaaat
     5941 cgtatcgtca cgtaaagcct tttgaatttg ctgggctgtg atttcttgat tttctggctg
     6001 gataatggtt gcttcaaacc caaagtgttg aaccaagtaa tcaattgttt caaggacagc
     6061 atggtgctcg atggcagttg tgatgatatg ttttccttgt tcttggtgac gaagacagta
     6121 gccaatgatg gtagtattat tgccttcagt cccaccagaa gtgaaaaaga tatgttgagg
     6181 ttttgtcctt agtaactggg ctagttcctg acgggcttct cgcaagagtt tgccagcttg
     6241 acgaccatga ccatgaatac tagaaggatt tccgtgggtt tcttgcataa ccttggtcat
     6301 agctgaaata gcaactgctg acataggagt cgttgcagca ttgtccaaat aaatcaaaga
     6361 atcaccttat ttctttttat tgtaggcaaa gagtgggctg actggttttc tttcgtgaat
     6421 acggacgata gcatcaccaa ttaactcact agcagtgatg tagcatacat ttttaggagt
     6481 tttttctttt gttgctactg aatcagtcac aagaatttct ttaatattag tattgtcaag
     6541 aagctcagca gctccctcga cgaagagacc gtggctagaa acagcataaa tttctgtagc
     6601 tccttcacgt tcaacgattt tagaagcttc agagaaggta cgtcctgtat ttaaaatatc
     6661 atcaatcaag atagctttct taccttcaac atcaccaata atataacctt cgttacgagt
     6721 tgcatcgtct tgagggtagt cgataatggc gataggagca tcaagatatt cagccaggct
     6781 acgcgcacgt ttgacacctg aatttttagg gctaacgaca acaacatctg aaccaagcaa
     6841 tcctttatcg cagtaatgtt ttgcgaatag gggaacagtg aaaagattat ccactggaat
     6901 atcaaagaaa ccttgaacct gaacggcatg caaatcaaga gtcaggatac gatcaactcc
     6961 agccttaacc agcatattgg caactagttt tgctgtaagt ggctcacgag gacaagcaat
     7021 gcggtcttga cgtgcatagc caaaatatgg aaggacaacg ttgatactgt gggcacttgc
     7081 acgcacacaa gcatcgacca tgattaacaa ttccattagg tggttgttga cagggaaact
     7141 tgttgattgg atgatgtaaa catcataacc acggacactt tcttcgatat ttacttggat
     7201 ttctccgtct gaaaattgac gtgatgatag ttttccaagt gggacaccaa cagcttgggc
     7261 aattttttgt gcaatctctt ggttagagtt gagtgcgaaa agtttcatgt tttttctatc
     7321 tgacattata gaccgtcctc tgtaaacttt ataaatccta gttatattta ccttacatat
     7381 atgaactggg atttgtgtat ttttatcttt tctattttac caaaaaatgg agattatttc
     7441 agctattttt catacttttg acaaatcgaa ccaattttga aggagctttt tggtaggaaa
     7501 tctgattttt ctctaaaaat tgtcggaaat cctgtttgcc ttgctcatga ttttccactt
     7561 caagctccaa ttcgtaatct gttatatcaa agtatcggct ctgatccagt gccatgagac
     7621 caatagctgt tttcatttca tagcgaagcg ttgttagaca accaagaacc tgccagttct
     7681 tactttggat accatgtttc gccaattcat ccagtactag cccttgagga agttcttcct
     7741 tactcagata gttctcagca tcttttagtt gcaatttttg gttgtattcc atgtttccaa
     7801 cactctgcgg gactttgagt gtcaactcag cccagtcttc aaaggttcga atgcgcatag
     7861 cgactttctt ttctcgcagt tcaaaatcag gcgtgtcgat gtagtaattt gtttgaagaa
     7921 caggagtgac acctgtgaac tggtctttta gacgattgta ttcatctttt ttcaatagtg
     7981 ttttcaattc aatttctaaa tgtttcattt ttcttacctt tttttatcgt tgaaagcgga
     8041 tttatggtat aataagcatt gtatttattg tatatgaatc tggagaaaaa atcaaagata
     8101 tttttgacgg ataatatgag aacaagggag aatatatgac cttagaatgg gaagaatttc
     8161 tagatcctta cattcaagct gttggtgagt taaagattaa acttcgtggt attcgtaagc
     8221 aatatcgtaa gcaaaataag cattctccaa ttgagtttgt gaccggtcga gttaagccaa
     8281 ttgagagcat caaagaaaaa atggctcgtc gtggcattac ttatgcgacc ttggaacacg
     8341 atttgcagga tattgctggc ttacgtgtga tggttcagtt tgtagatgac gtcaaggaag
     8401 tagtggatat tttgcacaag cgtcaggata tgcgaatcat acaggagcga gattacatta
     8461 ctcatagaaa agcatcaggc tatcgttcct atcatgtggt agtagaatat acggttgata
     8521 ccatcaatgg agttaagact attttggcag aaattcaaat tcgtactttg gccatgaatt
     8581 tctgggcaac gatagaacat tctctcaact acaagtacca aggggatttc ccagatgaga
     8641 ttaagaagcg actggaaatt acagctagaa tcgcccatca gttggatgaa gaaatgggtg
     8701 aaattcgtga tgatatccaa gaagctcagg cactttttga tcctttgagt agaaaattaa
     8761 atgacggtgt aggaaacagt gacgatacag atgaagaata caggtaaacg aattgatctg
     8821 atagccaata gaaaaccgca gagtcaaagg gttttgtatg aattgcgaga tcgtttgaag
     8881 agaaatcagt ttatactcaa tgataccaat ccggatattg tcatttccat tggcggggat
     8941 ggtatgctct tgtcggcctt tcataagtac gaaaatcagc ttgagaaggt ccgctttatc
     9001 ggtcttcaca ctggacattt gggcttctat acagattatc gtgattttga gttggacaag
     9061 ctagtgacta atttgcagct agatactggg gcaagggttt cttaccctgt tctgaatgtg
     9121 aaggtctttc ttgaaaatgg tgaagttaag attttcagag cactcaacga agccagcatc
     9181 cgcaggtctg atcgaaccat ggtggcagat attgtaataa atggtgttcc ctttgaacgt
     9241 tttcgtggag acgggctaac agtttcgaca ccgactggta gtactgccta taacaagtct
     9301 cttggcggtg ctgttttaca ccctaccatt gaagctttgc aattaacgga aattgccagc
     9361 cttaataatc gtgtctatcg aacactgggc tcttccatta ttgtgcctaa gaaggataag
     9421 attgaactta ttccaacaag aaacgattat catactattt cggttgacaa tagcgtttat
     9481 tctttccgta atattgagcg tattgagtat caaatcgccc atcataagat tcactttgtc
     9541 gcgactccta gccataccag tttctggaac cgtgttaagg atgcctttat cggtgaggtg
     9601 gatgaatgag gtttgaattt atcgcagatg aacatgtcaa ggttaagacc ttcttaaaaa
     9661 agcacgaggt ttctaaggga ttgctggcca agattaagtt tcgaggtgga gctattctgg
     9721 tcaataatca accgcaaaat gcaacgtatc tattggacgt tggagactac gttaccattg
     9781 acattcccgc tgagaaaggc tttgaaacct tggaggctat tgagcttcca ttagatattc
     9841 tctatgagga tgaccacttt ctagtcttga ataaacccta tggagtggct tctattccta
     9901 gtgttaatca ctctaatacc attgccaatt ttatcaaggg ttactatgtc aagcaaaatt
     9961 atgaaaatca gcaggttcac attgttacca gactagatag ggacacttct ggcttgatgc
    10021 tctttgccaa gcacggttat gcccatgcac gattagacaa gcagttgcag aagaaatcta
    10081 tcgagaaacg ctactttgct ttggttaagg gagatggaca tttggaacca gaaggggaaa
    10141 ttattgctcc gattgcgcgt gatgaagatt ccattattac cagacgagtg gctaaaggcg
    10201 gaaagtatgc ccatacttca tacaagattg tagcttctta tggaaatatt cacttggtct
    10261 atattcacct gcacactggt cgaacccatc aaatccgagt ccatttttct catatcggtt
    10321 ttcctttgct gggagatgat ttgtatggtg gtagtctgga agatggtatt caacgtcagg
    10381 ctctgcattg ccattaccta tccttttatc atccattttt agagcaagac ttgcagttag
    10441 aaagtccctt gccggatgat ttcagtaacc ttattaccca gttatcaact aatactctat
    10501 aaaaactgtc tcagagtata attattatct taaaggagaa aactcatgga agtttttgaa
    10561 agtctcaaag ccaaccttgt tggtaaaaat gctcgtatcg ttctccctga aggggaagag
    10621 cctcgtattc ttcaagcaac aaaacgctta gtaaaagaaa cagaagtgat tcctgttttg
    10681 cttggaaatc ctgaaaaaat taaaatttat cttgaaattg aaggaatcat ggatggttat
    10741 gaggtcatcg accctcaaca ttatcctcaa tttgaagaaa tggtttctgc cttggtggag
    10801 cgtcgcaagg gcaaaatgac tgaagaagat gtacgcaagg ttttggttga agatgtcaac
    10861 tactttggtg tgatgttggt ttacttgggc ttggttgatg gaatggtgtc aggagcgatt
    10921 cactcaacag cttcaacagt tcgcccagct ctacaaatca tcaaaactcg tccaaatgta
    10981 actcgtactt caggagcctt cctcatggtt cgtggtacgg aacgttacct atttggagac
    11041 tgtgccatta atatcaatcc agatgcagaa gccttggctg aaattgccat caactcagca
    11101 atcacagcta agatgtttgg catcgaacct aaaattgcca tgttgagcta ttctactaaa
    11161 ggttcagggt ttggtgaaag cgttgataag gtcgttgaag caactaaaat tgctcacgac
    11221 ttgcgtcctg accttgaaat cgatggtgag ttgcaatttg atgcggcctt tgttcccgaa
    11281 actgcagctc tgaaagctcc gggaagtaca gtagctggtc aagcaaatgt cttcatcttc
    11341 ccaggtatcg aggcaggaaa tatcggttac aagatggctg aacgcctggg tggctttgcg
    11401 gctgtaggac ctgttttgca aggtttaaac aagccagtta atgatctttc tcgtggatgt
    11461 aatgcagatg atgtttacaa gttgaccctc atcacagcag ctcaagcagt tcatcaatag
    11521 tgaaaactat aaagtgatat actatgctat actgtagtta tgaaactatg tacgaaaagc
    11581 actgccatta attcctgaga actaaattac tgattggtgt caaaaaggaa aacttccaag
    11641 cgatgatatc ctgtctatac acgacctata gaaatctgta atatacatat ccgtaaaacg
    11701 ataaattccc tttttgattt taaatgagta tgaaaagaga attttttggc tctttgtcaa
    11761 ctgtagtggg ttgaagt
//
LOCUS       DBEJAQ010000055        11674 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.055, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000055 DBEJAQ010000000
VERSION     DBEJAQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.055"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            485..1195
                     /gene="deoD"
                     /locus_tag="ACL297_001773"
     CDS             485..1195
                     /gene="deoD"
                     /locus_tag="ACL297_001773"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGR0071984.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1218..1742)
                     /locus_tag="ACL297_001774"
     CDS             complement(1218..1742)
                     /locus_tag="ACL297_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HGR0071985.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1795..2031)
                     /gene="rpsT"
                     /locus_tag="ACL297_001775"
     CDS             complement(1795..2031)
                     /gene="rpsT"
                     /locus_tag="ACL297_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGR0071986.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(2099..3019)
                     /gene="coaA"
                     /locus_tag="ACL297_001776"
     CDS             complement(2099..3019)
                     /gene="coaA"
                     /locus_tag="ACL297_001776"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGR0071987.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3209..3612
                     /locus_tag="ACL297_001777"
                     /pseudo
     CDS             3209..3612
                     /locus_tag="ACL297_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3819..4409
                     /locus_tag="ACL297_001778"
     CDS             3819..4409
                     /locus_tag="ACL297_001778"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGR0071988.1"
                     /translation="MSKMYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDF
                     GSQLLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEK
                     NNAKATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQ
                     KKQGAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4406..5683
                     /locus_tag="ACL297_001779"
     CDS             4406..5683
                     /locus_tag="ACL297_001779"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGR0071989.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5701..6363
                     /gene="deoC"
                     /locus_tag="ACL297_001780"
     CDS             5701..6363
                     /gene="deoC"
                     /locus_tag="ACL297_001780"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGR0071990.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6350..6739
                     /locus_tag="ACL297_001781"
     CDS             6350..6739
                     /locus_tag="ACL297_001781"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGR0071991.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6830..7882
                     /locus_tag="ACL297_001782"
     CDS             6830..7882
                     /locus_tag="ACL297_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HGR0071992.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            8026..9561
                     /locus_tag="ACL297_001783"
     CDS             8026..9561
                     /locus_tag="ACL297_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0071993.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGAINADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            9554..10612
                     /locus_tag="ACL297_001784"
     CDS             9554..10612
                     /locus_tag="ACL297_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071994.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10615..11571
                     /locus_tag="ACL297_001785"
     CDS             10615..11571
                     /locus_tag="ACL297_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0071995.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3332 a         2089 c         2669 g         3584 t
ORIGIN      
        1 taagactgac gaagtcagtc acatatataa tccaaggcga cgttgacgtg gtttgaagag
       61 attttcgaag agtataaaca gaaaggtaga gcgcgtgttc taatttgaac acgagtagaa
      121 aacttttcta aaaacaaaaa cgaaaggatg ggtaaactgt attcgctgaa ctgaatacgg
      181 gcgactctcc tctaaatcaa aattaagaaa gaaaggaatt gaccccaccc taaaagcagt
      241 gggaaaaaga tagttggtct agcgagcatc gctcactgcg cccaactcct attttccctt
      301 cgctttttga cgggtttggt atctttctca atataaaata taaaataaag aaaggtagag
      361 cgtgtgtttt gatttgaaca cgagcggaaa actcggaaaa tagataatct gactgaaaaa
      421 tcaggatttc tcgtcggctt cctaattttc agtcgttttc ttctcgctct ttgtatcata
      481 aattatgtct atccatattg ctgctcagca gggtgaaatt gctgataaaa ttcttcttcc
      541 tggggatcct cttcgtgcta agtttattgc ggagaatttc cttgatgatg ctgtttgttt
      601 taacgaagtg cgtaacatgt ttggttacac tggtacttac aagggtcacc gtgtatctgt
      661 catgggaact gggatgggaa tgccatctat ttcgatttat gcgcgtgagt taatcgtaga
      721 ctacggtgtg aagaaattga ttcgtgtggg aactgcaggt tctttgaatg aagaggttca
      781 tgttcgtgaa ttagttttgg cgcaggcggc tgcaaccaac tcaaacatcg ttcgtaatga
      841 ctggccacag tacgattttc cacaaattgc tagctttgat ttgcttgata aagcctacca
      901 tatcgccaaa gaacttggta tgactactca cgttgggaac gttttgtcat ctgatgtctt
      961 ttactcaaat tactttgaaa agaatatcga gcttggtaaa tggggagtca aggctgtgga
     1021 aatggaagca gcagctcttt actatcttgc tgcccaatac catgttgatg cgctagctat
     1081 catgaccatc tctgatagct tggtcaatcc agacgaagac acaactgcag aagaacgtca
     1141 aaataccttc actgatatga tgaaggttgg tttggaaacc ttgattgcag aataattata
     1201 gccaaaaagg aagcccttta tgaattgtag gacttccttt tcttatccag aaattgatct
     1261 agctctctct gatttcgaag aatagtgact ttatgtgaat attcttggca aagtttttgg
     1321 taattttctt tttgagtttt gctacgccca tcccaaagaa tccatctgat aaactcccac
     1381 tcaaagcgtt cagggcaatc tgccgccata ctttctctga cttttccacg gtatttaaga
     1441 taacgcttaa aggctctaaa gagacaggtc aatggcaaaa aattgagaaa gatgatttgg
     1501 tcagcttctt gcattcgttc ttggtagtag caccaagaat aattaccatc gatgacccaa
     1561 gctttatgct tggtgagaaa gttttttatc tcggttaaca tccattcgca gtcactgtct
     1621 tgccaaccag gttgaaattg gagtgtgtcc atgtgcagtt ttggaatgga gtagtagtta
     1681 gataactttt ctgctagagt tgacttacca gaaccagaat atccgataat tgtgattttc
     1741 attttctacc ttttcctatt tggagacaaa aaaacagcct ctatggactg tttcttattt
     1801 agcaagttta gttgaaagac gagctttatc gcggcttgct ttgtttttgt gaatcaaacc
     1861 tttagtttct gctttatcga tagctgagct agcagcacgg aaaagttctt cagatgggtt
     1921 tgcttcgaaa gcttttatag cagtacgcat agctgatttt tgagctgagt tcttttcgtt
     1981 ttgtttaacg ttcaattcag cgcgtttgat agctgattta atgtttgcca atgttcttac
     2041 ctccatattt actaactata ccattatata tgaaaactta cgttttgaca agggaaaatt
     2101 acttttttaa gtaaatttca tcgatttcat ggttcttgct tttatgaaga atcacttctg
     2161 cacgatttct ggttggttca atataatttt gcagatttgt gagattgata ctgatccaga
     2221 cctgatgggc aaaggactcc acttccccaa tcggcatctg agtaaaacga taatagtagc
     2281 tatcagggtc gttttgggct aggctcagca tcttcaagaa acggtccaga taccaacttt
     2341 caatatcatc tactccagca tctacataga tggaaaagtc aaagaagtca gtgatataga
     2401 gacgatcgtt ttgtggattt tgaaagacat taattccctc aacaattaca aaatcagcag
     2461 ctttgacact ttgtttcttt tcgggtacga tgtcgtaaac ttcatgagaa tagacaggaa
     2521 tatctacatc ttgtccattt ttgatgcggt ccaagaagtt gagaagagct tccatatcat
     2581 agctttcagg aaatccttta cgatttaaaa tcccctgctc aatcaaggtt tgattgggat
     2641 agagaaaacc atcagttgta accaactcaa ccgtagcatc tgtaaacgta cgggacagta
     2701 ggatttgaag taggcgactg gttgtggatt ttccaacggc aacactccca gaaaccccaa
     2761 taataaaagg ttgagattta ctttcacgtt ggaggaaaat tccttttgaa aaggctaaat
     2821 cttccttagt tcgcttgtaa atctgaatca gatgagccaa ggggagatag atatctgtaa
     2881 cgtcttggag actgatttgg tcattaaaac tcttgataga ttccaattct tcttctgtca
     2941 aaggaggtgt tgtctttcga tgtaaagatt gccaagtctg gcggctgatt ttttcaaaat
     3001 gtaaaaattc gttggtcatt tgttttcccc tagatatttt gtctatcata ccataaaaag
     3061 ctaaaaaaat aaagcggttt cttgaatgtg gtaagtcaat ggtatatagt ataagtgatc
     3121 aatttattga tgaaatagga gatagattct actgatttat aagcttataa agggggcttt
     3181 ttagttcaca acttatagga ggtgtactat gaaaatctta aaacgttaca tattggaact
     3241 ctgttttatt ttaagttttg ctttaccttt tataaaagga accaatgcag ataatggtag
     3301 atgctttgtg gaaacctatt acggttttac ttttttgatg gaacatgcta ttgtaacagc
     3361 tgtctttatc tgttcgttct taattgcttt cttactaaaa aacgatggac gaaatggatt
     3421 gctgcgggta gttattgctt tttagtccta cggattgcga cagagggtta tttcttccac
     3481 atgtcactag aagatttgat acgactttgg acaagtttgg aaatcctgac acaaacgtat
     3541 cagttgggct tttatctaaa cattttgtta ggaattttgt tgataataaa gtattttaag
     3601 gttaagcaat agaaataata atctgcctga ttatagatga taaacttttt gtttttatct
     3661 ggactgtgat atttatagtt ctaaatggtt tattatttat aagtgttgaa agagattgaa
     3721 gaaaatggct tcgtgacttg ctttcttcgg tctttttcgt tttattatca atttgtaaac
     3781 gatttacaat ttgagtcaaa ttatggtaaa atagtgtcat gagtaaaatg tactatgcag
     3841 aaaatcctga cgctgctcac gacattcatg agttgagagt ggacttgttg ggagaaaaaa
     3901 tgactttttt gacagatgcg ggtgttttta gcaagaaaat ggttgacttt ggaagtcaac
     3961 tcttgctcaa gtgtctggat gtcaaccaag gagagacagt cctagatgta ggctgtggtt
     4021 atggtccatt aggtttgtcc ttggtcaagg tttacggagt tcaggcgacc atggttgata
     4081 ttaacactcg tgccctggat ttagctcaga gaaatgctga aaaaaataat gcaaaagcga
     4141 cgatatttca atctaatatt tatgcaagag ttcaagggcg atttgaccat gtcatttcca
     4201 atcctcctat ccgtgcgggc aagcaagtcg ttcatgaaat catcgagaaa agtaaagatt
     4261 tcttggaaat tggtggagat ttgaccatcg tcatccagaa aaaacaagga gctccaagtg
     4321 ccaaatctaa gatggaagat gtgtttggca attgtgaaat ccttaaaaaa gacaagggat
     4381 actatatcct tagaagtgtg aaagaatgag agcagttgat ttaatccaaa aaaaacgaga
     4441 cggtcaagaa ctgacttcaa gtgaaattga atggctgata gaaggctatg tatcaggaac
     4501 tgttcctgat tatcagatgt ctgcctttgc tatggctgtt tattttaaag gaatgacgac
     4561 tcgagaaatc tctgatttaa cgatgaatat ggttaagact gggcaagagt ttgacctgtc
     4621 agccattgat ggtgtgaaag ttgacaagca ttctactggt ggtgtcggcg ataaggtaac
     4681 cttgatttta gctcctcttg ttgctagctt cggtgtgcct gtagcaaaaa tgagtggtcg
     4741 tggtcttggc catacaggcg gaacaattga taaattggag tccattaggg gctatcaagt
     4801 agaacgtagt caagaggatt ttattcgtca ggttcaggat attggagtat ctgtcattgg
     4861 gcaatcagac cagctggtca aagcagataa gcttctctat gcccttcgtg atgtgaccgc
     4921 aactgtcgac acgattcctt tgattgcgag ttcggtgatg agcaagaaaa ttgcggcagg
     4981 ggcggatgct attttgctag acgtgactgt cggtgagggt gccttcatga agacggttga
     5041 tgaggcgcgt gagttggctc aaactatggt agatcttggt aaggttgttg gacggaagac
     5101 agtagcagtg attacggata tgagccagcc tttggggcga gcgattggaa atcgtctgga
     5161 aatccttgaa gcattggaga ttttgcaagg tcaaggacgt caggatatta cccactttat
     5221 ttgtgaattg gctcaaatta tgcttggttt ggcaaatgtt aacaaaactg ttgaagaagt
     5281 tcgccaacat cttgaaaatg gtcaagcact ggctaagttt gaggaaatgg tgcaagccca
     5341 aggtggagac ttggaagacc tctatcgtcc tgtaaatgta gctcatgtgg tggaaatccc
     5401 tgctcaggaa acgggtgtca tttcagctct tccagctatg gactttggtc tttatgccat
     5461 gaggctgggt gctggtcgtg cagttaagtc tgatgtcttg gactatgaaa caggaattgt
     5521 ttttgaaaag aagattggag actccgttca aaagggtgaa attgttgcaa aagtatacac
     5581 aaatgaaaaa attccacctc aactagttac agatttccaa aaatgtgtta aaataagtga
     5641 tgaagtgaaa aaaatccgtg aaattgtaga aatcatctcc taaatggagg gaaaaccgaa
     5701 atgaaattaa ataaatatat agatcatacg cttttaaaac aagatgcaaa gaaaaaacaa
     5761 attgatagtt tgttgtctga ggctagagag tatggctttg ccagtgtttg cgttaatccg
     5821 acctgggttg aacatgctaa aaaaggactt gaaggcacag atgttaaggt ttgcacagta
     5881 gtaggtttcc ccttgggagc aacaacttca actgtgaaag cttttgaaac aaaagaagct
     5941 atccaaaatg gggctgatga gattgacatg gtaatcaatg ttggggctct caagtcaggt
     6001 gatttggcct tggttgagtc ggatattcgt gcagtagtgg aagcaagtgg ggacaagtta
     6061 gtgaaagtta ttattgaagc ttgccttctg acagaccaag aaaaagtttt ggcttgccaa
     6121 ttagcccaaa aagcaggagc tgactttgtc aaaacatcta ctggcttttc aactggtggt
     6181 gctacgatag cagatgttag attaatgcgt gaaacagttg gacctgatat gggtgtcaag
     6241 gccgccggtg gagctcgttc ttatgcagat gctcttacct ttgtcgaagc aggtgcgacc
     6301 cgtatcggaa cgtcagctgg ggtagctatt ttaaaaggag aattggcaga tggcgactac
     6361 tgagttgatt gaactggcaa ttgaaaccag caaacatgcc tatgtcccct attctcactt
     6421 tcctatcgga gcagttttag tagccaaaga cggaagtgtt tacacgggag tgaatatcga
     6481 aaatgctagc tatcctttga ccaattgcgg tgagagaaca gctattttta aggctatatc
     6541 tgaaggccaa agagaatttt cagaattgat tgtctatggt caaactgaaa aaccgatttc
     6601 accatgtggt gcttgtcgcc aagtaatggt cgaatttttt gaacaagatt taaaagtgac
     6661 cctagtcgca aaagataaat cgacggtcga gatgacggtc ggggagttac ttccatactc
     6721 ttttacagac ttaaattagt ctgagtcgct ctttgagtgg caagggtctt tgtgaccaat
     6781 caatccatac ttgcaacatc gttgcacatc ttatttagga ggttcagtaa tgaacaagaa
     6841 acaatggcta ggtcttggcc tagttgcagt ggcagcagtt ggacttgctg catgtggtaa
     6901 ccgctcttct cgtaacgcag cttcatcttc tgatgtgaag acaaaagcag caatcgtcac
     6961 tgatactggt ggtgttgatg acaaatcatt caaccaatca gcttgggaag gtttgcaaga
     7021 ctggggtaaa gaacacaatc tttcaaaaga taacggtttc acttacttcc aatcaacaag
     7081 tgaagctgac tacgctaaca acttgcaaca agcggctgga agttacaacc taatctttgg
     7141 tgttggtttt gcccttcaca atgcagttga agaagcagct aaagaacaca ctgacttgaa
     7201 ctatgtcttg attgatgatg tgattaaaga ccaaaaaaat gttgcgagcg taactttcgc
     7261 tgataatgag tcaggttacc ttgcaggtgt ggctgcagca aaaacaacta agacaaaaca
     7321 agttggtttt gtaggtggta tcgaatctga agttatctct cgttttgagg caggattcaa
     7381 ggctggtgtt gcgtcagtag acccatctat caaagtacaa gttgactacg ctggttcatt
     7441 tggtgatgcg gctaaaggta aaacaattgc agccgcacaa tacgcagccg gtgcagatat
     7501 tgtttaccaa gtagctggtg gtacaggtgc aggtgtcttt gcagaggcaa aatctctcaa
     7561 cgaaagccgt cctgaaaatg aaaaagtttg ggttatcggt gttgaccgtg accaagaagc
     7621 agaaggtaaa tacacttcta aagatggcaa agaatcaaac tttgttcttg tatctacttt
     7681 gaaacaagtt ggtacaactg taaaagatat ttctaacaag gcagaaaaag gagaattccc
     7741 tggcggtcaa gtgatcgttt actcattgaa ggataaaggg gttgacttgg cagtaacaaa
     7801 cctttcagaa gaaggtaaaa aagctgtcga agatgcaaaa gctaaaatcc ttgatggaag
     7861 cgtaaaagtt cctgaaaaat aatggatgga agtcatttct taggaagcgg ccaagggccg
     7921 cttttttaag agttgagaca aagtcatttt gtctcagtaa agcttgctac tagacaaact
     7981 agcaagtttt attgaaataa aataagactc tgaaaggaag agcacatggc acacgaaaat
     8041 gtcattgaga tgcgtgatat taccaaggtg tttggtggat ttgttgccaa cgacaaaatc
     8101 aacttgcacc tacgaaaagg tgaaattcat gcacttttag gagaaaatgg ggctggtaag
     8161 tccacgctaa tgaacatgtt agcaggcctt cttgaaccaa ccagtggtga aatcgcggtc
     8221 aacggtcaag ttgtcaatct cgactcccca tctaaagcag ctagcttggg aatcgggatg
     8281 gttcaccagc actttatgtt ggttgaagcc ttcacagtgg ctgaaaacat cattttaggt
     8341 agtgaattga ctaaaaatgg tgtgctagat atcgctggag ctagcaaaga aatcaaggct
     8401 ctttctgaac gttatggctt agctgttgac ccttctgcca aggtagcaga tatctcagtt
     8461 ggagcccaac aacgtgtaga aattttaaaa acactttatc ggggggctga tatccttatc
     8521 tttgacgaac caacggctgt tttgactcca tcagaaattg atgagttgat ggctattatg
     8581 aaaaatcttg tcaaagaagg aaaatcaatt atcttgatta cccacaaatt ggatgaaatt
     8641 cgcgcagttt ctgaccgtgt tacagttatc cgtcgtggga aatcaattga aaccgttgaa
     8701 attgcagggg ctatcaatgc tgatttggcg gaaatgatgg taggacgttc tgtttccttt
     8761 aaaacagaga agcaagcctc taaaccaaaa gaagtggttt tgtctatcaa agatttggtg
     8821 gtcaatgaaa accgtggtgt tccagctgtt aaaaatctgt ccttggatgt tcgtgctgga
     8881 gagattgttg gtattgcggg gattgatgga aatggtcagt ctgaactgat tcaagccatt
     8941 acaggtcttc gtaaggttga atctggtagc attgagctaa aaggagattc aattgtaggc
     9001 ttgcacccac gtcagattac agaactaagt gttgggcacg ttccagaaga ccgtcaccgt
     9061 gatggcttga ttttggaaat gatgatatct gaaaatattg cccttcaaac ctactataaa
     9121 gaaccacata gtaaaaatgg aattttgaat tattcaaata ttacttctta tgctaaaaag
     9181 ctgatggaag agtttgatgt tcgcgctgcc agtgaattag ttcctgcagc tgcactctca
     9241 ggaggaaatc aacaaaaagc aattattgct cgtgaaattg atcgagatcc tgatctcctt
     9301 atcgttagcc agccaactcg tgggttggat gtcggtgcca ttgagtatat ccacaaacgc
     9361 ttgattgaag agcgtgataa tggcaaggct gtccttgttg tcagctttga attggatgag
     9421 attttaaacg tctcagaccg tattgccgtt atccacgatg gtaagattca aggtattgta
     9481 tcaccagaaa caaccaataa acaaggactt ggtgtcttga tggctggtgg aaacttggga
     9541 aaggagaaga gtgatgtcta aaaaattaca acaaatttcg gttcccttga tttctgtatt
     9601 cctaggaatt ttactcggag ccattgtcat gtggatcttc ggttatgatg ctatttgggg
     9661 ctacgaagaa ttgttctata cagcctttgg cagtctgcgt gggattggag aaatcttccg
     9721 tgctatgggt cctctggtct tgattggtct tggttttgcg gttgccagtc gagctggttt
     9781 ctttaacgtc ggacttcctg gtcaggcttt ggcaggttgg attctcagtg gttggtttgc
     9841 cctgtcgcat ccagatatgc cccgtccctt gatgattcta gcaaccatcg tgattgcctt
     9901 gattgctggt gggattgtcg gagcgattcc aggtattctt agggcctatc tagggacgtc
     9961 agaggttatt gtaaccatca tgatgaacta cattgtcttg tatgtaggga atgcctttat
    10021 ccatgctttc cctaaagact tcatgcaaag tacagattcg accattcgtg ttggggctaa
    10081 tgcaacctat cagacacctt ggttagctga gttgactggt aactcacgga tgaatattgg
    10141 tattttcttt gccatcattg ccgttgcagt tatttggttc atgctcaaga aaacaactct
    10201 tggttttgaa atccgtgcag ttggtcttaa tccacatgct tcagaatatg ctggtatttc
    10261 tgccaagcgg actattatcc tatctatgat tatttcaggt gccttggcag gtcttggtgg
    10321 agctgttgaa ggtttgggaa ccttccagaa cgtctatgtt caaggttcgt cattagctat
    10381 cggatttaac ggaatggcgg ttagtttgct tgcggccaac tcaccaattg gtatactctt
    10441 tgcagccttc ctatttggcg ttctccaagt tggggctcct ggtatgaatg cggcgcaggt
    10501 accatctgag ctcgtcagca ttgtaacagc gtctattatc ttctttgtca gtgttcatta
    10561 ccttatcgaa cgctttgtca aaccgaaaaa acaagttaaa ggaggtaagt aaagatgtct
    10621 attataacct tgctccccct cttggtgtct tctatgctga tttactcagc acctctcatc
    10681 tttacaagta tcggtggtgt tttctctgaa cgtggtggtg tggtaaacgt tggccttgaa
    10741 ggaattatgg ttatgggtgc cttttctgga gttgtcttta accttgaatt tgcagaacaa
    10801 tttggagcag caactccatg gctatccttg cttgtagcag gattggttgg tagtgttttc
    10861 tctatcatcc acgcagcagc gacggttcat ttccgtgcag accatgttgt cagcggtacg
    10921 gtattgaact tgatggcgcc tgccttggct gttttcttag ttaaagttct ttataacaaa
    10981 ggacaaaccg acaacctaag tcaaactttt ggacgctttg atttcccagt cttggcaaat
    11041 atcccagtga ttggtgatat cttcttcaag tcaactagtc tacttggtta tctggcgatt
    11101 gccttctcat tccttgcttg gtttattctc tttaagactc aatttggtct tcgtctccgc
    11161 tctgtcggtg aacatcctca agcagcggat accttgggaa tcaacgtcta caagatgaga
    11221 tatttagggg ttattatttc aggttttcta ggtggaattg gcggagcgat ttatgcgcaa
    11281 tcaatctcag ttaacttctc agtgacaact attgttggac ctggatttat cgcccttgct
    11341 gcgatgatct ttgggaaatg gaatccaatc ggtgctatgc tttctagtct cttctttgga
    11401 ctttcacaaa gtttggctgt tatcggttct caattgccgt tcctacaagg agtgccagcg
    11461 gtttatcttc aaattgcacc ttatgttttg acaattcttg tcttagcagc cttctttgga
    11521 aaagcagtcg caccaaaagc agatggtatc aactacatca aatcaaaata agcatacaaa
    11581 aaaacgtcag tccaatgact gaactgcccc ccaaaagtta gacagaaaaa atctaacttt
    11641 tggggtgttt ttattatgaa attaacttat gatg
//
LOCUS       DBEJAQ010000056        11613 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.056, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000056 DBEJAQ010000000
VERSION     DBEJAQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.056"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(143..994)
                     /gene="rgg"
                     /locus_tag="ACL297_001786"
     CDS             complement(143..994)
                     /gene="rgg"
                     /locus_tag="ACL297_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HGR0071996.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNVAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFYTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            1118..1459
                     /locus_tag="ACL297_001787"
     CDS             1118..1459
                     /locus_tag="ACL297_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGR0071997.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            1523..1687
                     /locus_tag="ACL297_001788"
     CDS             1523..1687
                     /locus_tag="ACL297_001788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0071998.1"
                     /translation="MKIKGQTRKLAAGCSKHCFEVMDRTDEVSPKHCFEVVDRTDEVS
                     NHTYGKATLT"
     gene            complement(1713..3416)
                     /gene="ilvD"
                     /locus_tag="ACL297_001789"
     CDS             complement(1713..3416)
                     /gene="ilvD"
                     /locus_tag="ACL297_001789"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGR0071999.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(3649..4581)
                     /locus_tag="ACL297_001790"
     CDS             complement(3649..4581)
                     /locus_tag="ACL297_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGR0072000.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(4578..5435)
                     /locus_tag="ACL297_001791"
     CDS             complement(4578..5435)
                     /locus_tag="ACL297_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGR0072001.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(5439..6785)
                     /locus_tag="ACL297_001792"
     CDS             complement(5439..6785)
                     /locus_tag="ACL297_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGR0072002.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(6798..7082)
                     /locus_tag="ACL297_001793"
     CDS             complement(6798..7082)
                     /locus_tag="ACL297_001793"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0072003.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(7086..9116)
                     /locus_tag="ACL297_001794"
     CDS             complement(7086..9116)
                     /locus_tag="ACL297_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGR0072004.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEYYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            9307..10314
                     /locus_tag="ACL297_001795"
     CDS             9307..10314
                     /locus_tag="ACL297_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGR0072005.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            10317..10517
                     /locus_tag="ACL297_001796"
     CDS             10317..10517
                     /locus_tag="ACL297_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGR0072006.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            10669..10851
                     /gene="rpmF"
                     /locus_tag="ACL297_001797"
     CDS             10669..10851
                     /gene="rpmF"
                     /locus_tag="ACL297_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGR0072007.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            10867..11016
                     /gene="rpmG"
                     /locus_tag="ACL297_001798"
     CDS             10867..11016
                     /gene="rpmG"
                     /locus_tag="ACL297_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGR0072008.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(11508..>11613)
                     /locus_tag="ACL297_001799"
     CDS             complement(11508..>11613)
                     /locus_tag="ACL297_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGR0072009.1"
                     /translation="VSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3758 a         2443 c         1911 g         3501 t
ORIGIN      
        1 tatagcactt atgaatttgc gaaacgattc ttttgaatat atgcaacaaa caaaatgagc
       61 aaaaaaacga tagaactttt gtaatgacct gagagagggc aacagttcta tcgcttcaaa
      121 ttttttcttg gtttgcagat actcaacatt cggtcgtatt tctatagtat tcggcagatt
      181 tattacagcc aagcatctca aaaatacgga cagcatcctc catctttttc tgaccttcct
      241 tgactctacc ttgcttgcta tcaaggagac cttctgccca cagataaaca attcggaaat
      301 aggtctcatt ttccttgtag aaatgctctt cgataacacg tttaaaataa taggcattgg
      361 taaattcttc acactcaata ctagctaaaa agccattcaa tagtatagta taaaaaaggt
      421 ttcgattgcc agacatttcc attagaaaat cggatttacg taccatttct cgtacatatc
      481 tagtaaaaag agaaacagat aaaaatggag aactgactga aaataaattg agttcataga
      541 ttccccagat ctcggtagaa aacaaataat catgaaggac ttttccttcc tctgctgtta
      601 actctaccct ttcatccatg ctcttcatat aagacttgat cataatggca tttagaatat
      661 gtttctgttt gttgtgagaa tgggcatgct tttgatactc cctgcgatat aagtcctcaa
      721 gaggtgctac attctttggt tccaagacat ctgtaatttc ttttctcaac tcagaatctg
      781 tatcatactg gaaacctctc gccagaaaga ggatctcctc cacactggca gatatatttt
      841 ccagagcaaa tagaaacttt tccaccgaaa gctcactctg acctgtttca aaacgggaca
      901 acatagacgg cgaaaattgt cctccggttg cttgtctcag tgagatattt cttgactctc
      961 gtaattgtct aaagactttt ccaatctgct ccatagactt ccccttgatt ccgtattttc
     1021 ttcattttat catatttttc agaaaattca tcaaaaactt gccaaattgt cagaattatg
     1081 agaaaataga ggatatttat cacgtggagg gactgctatg agagacgata tcaaaatcaa
     1141 tgaccgtgct ttggccttgc aagaccaaat catcgaaaaa ctagagaaag tttttgatac
     1201 agatgtggaa ttggatgttt acaatctagg tctgatttat gaaatcaatc tggatgaaac
     1261 ggggctctgc aagattgtca tgaccttcac cgatactgcc tgtgattgcg ccgaaagcct
     1321 gcctattgaa atcgtggcag gtctgaaaca aatcgagggt atcaaagata tcaaggttga
     1381 agttacctgg tcgcctgctt ggaaaatcac acgaatcagt cgctatggcc gtattgccct
     1441 tggactacca cctcgttaag caggccaatc atttttaaag atgaaaaaag caagccaaaa
     1501 atggcttgct ttttgatact caatgaaaat caaagggcaa actagaaaac tagccgcagg
     1561 ttgctcaaaa cactgttttg aagttatgga tagaactgac gaagtcagcc caaaacactg
     1621 ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat acctacggca aggcgacgtt
     1681 gacgtgattt gaagagattt tcaagtatga gtttattttt tacctgactt gtccatattc
     1741 cagaagtctg tcacggctcc gcgtgaagca gatgatacga tatgggcata tttaccgagg
     1801 acaccacggc tgtaaagtgg tggcaaggtt gtttctgcct tgcgtttttc aagttcttct
     1861 tcggatacgg ccatggaaat ttctttggta tcttggtcaa ccgtaacgat atcgccggta
     1921 cggagatagg caattggtcc accatcctga gcttcaggag cgatatgtcc aacaaccaga
     1981 ccataagtac caccagagaa acgtccgtcc gtcaagaggg ccaccttatc tccctgacct
     2041 ttaccaacaa tcattgaaga aagtgacagc atctcaggca taccaggacc acctttaggt
     2101 ccaacaaaac gaacaacgac tacatcgcca tcaacgattt catctgtcag aacggcctga
     2161 atcgcatctt cttctgagtc aaagacctta gctggcccaa cgtgacgacg cactttaaca
     2221 cctgatacct tggcaactgc accgtcagga gcaaggttcc cgttcaagat gataagcgga
     2281 ccatccgcac gttttggatt ttcaagtggc atgataactt tttggcctgg agtcaagtct
     2341 gcaaagtcag ccaagttttc agctacagtc ttaccagtac atgtgatgcg atctccgtga
     2401 aggaaaccat ttgccaacaa gtacttcata accgcaggga caccaccgac ttcgtagagg
     2461 tcttggaaga catactgacc agatggtttc aagtcggcca agtgaggcac acgttcttga
     2521 atcgtattga agtcctcaag tgacaagtca acatttgcgg catgggcaat ggcgagcaag
     2581 tgaagagtgg cgtttgtaga accaccgaga gccatcgtta cagtaatagc atcttcaaag
     2641 gcttcacgag tcaagatatc tgatggtttg agaccaagtt ccaacatctt aacaacagca
     2701 cgtcctgctg cttcgatatc ttctttctta tcagctgatt cagctgggtg agaggatgac
     2761 cctggcaaac tcatccctag aacttcgata gcagttgcca tggtattagc agtatacata
     2821 ccaccacaac caccagggcc agggcaggca ttacattcaa gacgtttcac gtcctcagct
     2881 gtcatgtcac cgtggttcca ttttccgata ccttcaaaga cagaaaccaa gtcgatatct
     2941 ttaccatcaa gatttcccgg tgcaatagtt ccaccatagg cgaaaatagc tgggatatcc
     3001 atattagcaa tagcaatcat agatccaggc atgttcttgt cacagccacc gatagcgacg
     3061 aaggcatcca cgttgtgacc actcatagcc gcctcgatgg agtccgcgat gatgtcacga
     3121 gatgttagag agaaacgcat accaggcgtt cccatagcga tcccgtccgc tacggtaatg
     3181 gttccaaact gtacaggcca agcgcctgca gatttgacac cttctttagc cagtttcccg
     3241 aaatcatgca agtgaatgtt acatggtgta ttttccgccc aagtcgaaat cactcccaca
     3301 atcgatgttt caaagtcctt atctgtcata ccagtcgcac gaagcatagc acggttaggt
     3361 gatttaacca tgctgtcata gatgctactg cggtgacgtt tatctaattc agtcatctta
     3421 tccctcccat ttcagttttt actattatag cacaatttaa aggcaatgaa cagaataaaa
     3481 ttcttgaatt ttcagaaaat tctatacgca tgtgaaatat ttaaaattaa aaacaacaaa
     3541 gcggattagt gcactttctg atgaccagaa tatgcttttt aatccgcttt ctttaaataa
     3601 cgtactgtaa tttttacaga aattctttca gataagtgta tttaacatct atcttgcatt
     3661 ataaatttct agaaccttct cttttatatt cgattcactc aaaccatact cattaagaag
     3721 ataatccatt ttccctactt gaccgaatct ttcttgaaca cccatccgat gaatttttgt
     3781 tattccatca tcagagaata attcacataa agcactgcca attccaccta tctgattgtg
     3841 gttttctaca gtaaatatag tttttccact taacattgtt tttatctgtt ctggtatcgg
     3901 tttgattcta aataaatcta tcacacttac tgaataacct aatttagaca gttcatctgc
     3961 aactcgaata cttggagcaa ccattatacc agaagcaacg attacaagat cttcaccatg
     4021 ccttaactca atgtagcctt tagaaaaatc ttctccacct tgatacacag gaactggagc
     4081 ttttctaatt gttcgaatat attttagtcc ttttaagtct aatgtctggt tcaatatttc
     4141 acgaaattgg atatcatcag ttgcttcaaa aatgatcgat ttaggaatta aacgtaacaa
     4201 tccaatttct tcaaatggca tatgtgtccc accattcatt tctgcagtta ctcctgcatc
     4261 tgatccaatc acagtggcat ccaattgtgc gtatccaaga gaaataaata attgatcaaa
     4321 tactcttcgt gaagcaaaag gaccaaatgt atgaagataa ggtctaaacc cctgaataga
     4381 caagcctgct gcaaggccga ccatttctgc ttccataatc ccaacattca cataacggtc
     4441 tccaaagtcc ttttcaagat tatgagtagc catcgaactt gacaaatcgg cttctaagac
     4501 tactatatca gaatcacttt gattagcctc taaaaggaag tctctatata catgccgtaa
     4561 ttctttcgtg cttctcatca ttctgtttcc tccaattcct gacttaatct ttctacaact
     4621 gaggttaaca tttgtctctc ctctacagta gggcgaagat gatgattgga tttcatttct
     4681 tccagctctt gaaccccttg acctttaata gtatctaata caatgcactt aggtgatgaa
     4741 ttatttgact gttttaattg gacaatccct tcataaattt ctctaatatc tgaacccttg
     4801 accctaatgg attcaaatcc aaatgctgaa aatttttcta cgaaatcacc tggattacaa
     4861 atatcctttg taaaaccatc taattgtttt ttgttatcat caacaaatac aattaagttg
     4921 gataactgtt gatgagaagc aaactgtata gcctcccaac attgtccctc atttaactca
     4981 ccatctccaa caatagcata agtataaaag ggactctttc ttattctctg accatatgca
     5041 agtccagttg caacactaat tccttgtcct aaagagcccg ttgtcatatc tatgcctggc
     5101 gttagatttc tatcaggatg agacggtaat ttggttccat ttgtatttaa agaatataag
     5161 aattctttgt caaagaaacc attcaaatag agtgtactgt atagagctgg tcctccatga
     5221 ccttttgata atatgaaata atctctatct cgtgctgcaa atatttctgg agtcattggc
     5281 attatttcac cataaagcac tgctaaaact tctacgatag acagacttcc tccgtaatgt
     5341 ccgaatccaa gatgattcaa tgttctaaga gtatttaatc ggatgttagt cgcaaatttt
     5401 cttaacccat cttctctatt tttacttaaa atcatccctt attcctcctt tgcagatggc
     5461 tttttaataa aggatactcc aaacataact gctagaataa gaacaagacc aatcacaatg
     5521 cctgcttgtg agccaaattg atttaacatt cctaaaataa ttcctgatag accaaaatct
     5581 gcatctgaga aagttgatcc ttggaaacca agtcctccca aaactggcat taaaaagact
     5641 ggaagaaaac tgattaaaat accttgtaaa aatgctccaa tagtggctcc acgaacacca
     5701 ccagatgcat tcccaatgac acctgcagtc gctccacaga agaaatgagg cacaacacct
     5761 ggtaagataa caaccgttcc tgaagcaatc ataattgcca tacttactaa accaccaata
     5821 aaactagaga taaatccaat tagaactgca ttgggtgcat aagtataaac aatcggacaa
     5881 tccaaagcag gttttgaatt aggtacaaga cgctctgaaa tacctttaaa ggctggaaca
     5941 atttcgccca aaataaggcg aacacctgct aaaataacaa atacccctgc tgcaaattga
     6001 cctgctaatt gtaaagcata aactagacca cttgtaccac tactgatttc tttttctata
     6061 tattctgacc ctgcaaagat agctacaata atgtaaataa ctgccatgga taaagtaata
     6121 ctaacagtac tatcacgtaa aaaagctaaa ctctttggaa atttaatgtc ctctgttgat
     6181 tttgatttgt caccgataag gctaccagta aaaccactca accaatatcc caaagaactg
     6241 aaatgaccta aagccacctt gtcatttcca gttaattgaa ccatatattt ttgaacaaat
     6301 gctggggaaa tactcataat aataccgagt gctaatcctc ctagtaagat aagaggcaag
     6361 ctagtaaagc cagcaactga taaaatgacc gcaatcatac atgccatata tagagtgtgg
     6421 tgccctgtta aaaaaatata tttaaatcga gtaaaacgag cgattaagat attgaacacc
     6481 atgcctgcaa acataatcag tgcagtagct gagccatatg ttgttaaagc tacagctaca
     6541 attgcttcat tattcggcac aacgccagat aaatgaaaag catgctcaaa catggtacca
     6601 aatggattta aagaattttg tacaattcct gcaccaccag atacaactaa gaaaccaaca
     6661 aaggtcttaa ttccaccttt aataatatca ggtaatttct tcttctgaag aactaatcct
     6721 aaaattgcaa ttaaagctac taaaatagct ggtgtactaa caatatccaa tatgaacttc
     6781 atcatgacgc tagcctccta tataagtcct ttttcttcac aaagtttagt aattaattct
     6841 cgtagttcat ccatatcaat aatactattt aagatacgaa catctccaag atgactagct
     6901 gaatcagcta gatcacgacc aacaatccaa atatcagctg catttggatc tgctccacct
     6961 aaatcataat gttcaacttc tacatctgaa acattcaaat cactcaatac agattcaata
     7021 ttcatctgta ccataaaact tgaacctaat cctgaaccac aagctgtacc aatttttaac
     7081 attatctaat cctcctgttt aattatcatt ttaatgtcat catagttttt tgatgatatt
     7141 aaagtttgaa catgattttt atctcttaaa attgttgtta aatgtgacaa agcctttaaa
     7201 tgactctcat tatcaatggc tgcaatacaa atcaacaatc ttacctcttg ttctggatta
     7261 tccaataaat aaatcggttc ttccaaaact aacattgaca ttcctatttc attcacacct
     7321 tcatctggcc gagcgtgagg aattgctact cccttcccta aattaataaa aggcccaaac
     7381 tcttctactt tttgaatcat tgcctcaggg tagttctcag ttatcttatc ttgatccaaa
     7441 agcggtttag ctgctaaacg aatcgcctcc ttccatccta atttttgcga actaacctga
     7501 taggtttctt tggtaatgag ttgttctagc actggtacaa tttcctttct atcatttttt
     7561 tggtaaagat aattctttaa cgccaatctt aattccaatt cttgtgtaat aattccatat
     7621 cttttgacaa tattcagaat ttgttcaatc tcaaaatccc catactctaa attcggaaaa
     7681 tcttttaaca ctagttctac tagttgtatt gcttgctctt cagtcatcat aaccgaaact
     7741 agataatttg gcttttctgt ctccaccttt atggtagaaa aaaccatatc atagtcacta
     7801 ctagctttca cctgtaaatc atcaatcttt gaggttccta taaactcaat ttgaggaaat
     7861 aatgctaata gattctcttt taacatcaat gaagaactaa caccattagg acaaatgatt
     7921 gctgctttat accatttttg aggcaaagta tctgctttct ttaaataacc tccgaaatgg
     7981 ataacaaaat atgctgtttc actatcaggt atgggattgt caatagcgtc catcaagggc
     8041 atcaaagaat ctttgactaa ttcaaataaa tcaggataat attctttaac atgcaataca
     8101 tattcatttg aactaggtag gccgaacttt aatctatagt aagccggtat aaggtggcgg
     8161 cgaagatttt ctctcaatcc ttccctttgt ttaaaatgta acaaagaaat atcttccatt
     8221 ctacttataa tagcctctgt taattgatta aagtaaaccg gagcaacatc tacttcacct
     8281 tcaaagcaac ttgataataa aactgtgata tagcgataat catcctcaga aaacaccgta
     8341 tctataattc ccaaatcaac cactgtatcc aataaaatag tggttatatc ttgaataaca
     8401 ggagatacta atgtctctga aagacatact ctttcaacat ccctttgata cctacacaga
     8461 atgaatacta aaccgaaaag gcaaactttt aattgattaa caataggtac tagttgtagc
     8521 ttctcataat aatctttaac tacctgattt atcaaatcat aagttaatga atacccccaa
     8581 ctggataaaa cataatccaa accccaaatc cctatggagg attccagcaa ctcactaacc
     8641 atttgaaaag ctaagcggtg cttattccac tctgaaccgt gtaaagtata accttttgct
     8701 ctactgtacc ctagctccaa atcattatct aacataatct ttcttaatga ttgaatatca
     8761 gataaggttg tattcttact tactttcaaa aagtcttggt aatgattatt cgatataaaa
     8821 tctaatcggc aaaaagtgta aagatagatt aaagctaagc gagtcgactt tggtaaaacc
     8881 aattcatccg acttaataat atctgtcaaa gactgcttcg tacgatttga taaactatag
     8941 cgaccttgtt ttttatccag cactatccct ttattagcta gataaggcac taaataatct
     9001 attccttctt tgacttcctt tataggtaag ctcaccttaa cagataattc atataatgat
     9061 agctcacaat gatccatcaa agtcatcaaa ataactagtg ctctataatc aaacatttct
     9121 cttcctttct aactacaatt atagtgtaaa aattataaga ataaaagagt atttgagcac
     9181 aatataatct caccttttaa aagaaattat tgggctgaaa gtttttagtg attattttca
     9241 actttctatt gacttttaaa aacaaagtga tatcatttag atatcaaata atgggaggtc
     9301 attcttatga ctgaaaaact aatcaattca aaaccaaatg gtgtattcgc attgattctc
     9361 attgagttga caatcgtact tggtgtcttt atatttataa tgggtgttgg ttcggaaaac
     9421 atttttggaa ttattatcgg acctttacta atcgtaattg caggtctagc tcatgctggt
     9481 ttaaaagttg tcaaacctca ggaagctctg gttctgacac tctttggtaa ctatacaggt
     9541 accatcaaag aacctggttt ttactttgtc aatcccttca gcgtagcagt caaccctgca
     9601 aaccacactc gacttggaca aagtggtgat gttagcacaa aatctccttt tttaggagct
     9661 aaatcatcaa atgacaatga tgtaaatctt gaaattggca agaaacagat ttccctcaaa
     9721 gtcatgacct tgagcaattc tcgtcaaaaa atcaatgatt gcttaggaaa ccctgtagaa
     9781 atcggtatcg cggtaacttg gagagttgtc gataccgcta aggcagtctt caacgttgat
     9841 aactacaaag aatatctttc attgcaatgt gatagcgccc tccgtaatat tgtccgcatc
     9901 tatccttacg atgtgtctcc taatgtggat actacgggtg acgggcaagc agatgaaggt
     9961 agtctccgtg gctctagcga aattgttgct aaccgtattc gtgaagaaat ccaaagtcgt
    10021 gttgaggatg ctggcttgga aatccttgaa gcacgtatca cttacctagc ttatgctcca
    10081 gaaattgctg ccgttatgct tcaacgccaa caagcatctg ccattattga tgcacggaag
    10141 atgattgtag atggtgctgt aggaatggtt gaaatggcac tagaacggct caatgaaggg
    10201 gaattggtag aacttgacga agaacgaaaa gctgccatgg tttcaaatct ccttgtcgtt
    10261 ctttgtggca atcatgatgc acaaccaatt gtcaacacag gaagtcttta ctaaagatgg
    10321 ctgaagctaa aaaaaagcag atcccccttc gactctcaac aaagttatac gctgcactcg
    10381 catcatgggc agaagacgac tttcgttcag tcaatgggca aatcgaatat ctcctcacag
    10441 agtgtgtcaa gcaacggaag aaagacggaa aatacgtatc agaaaccatt gacgaaccat
    10501 ttgagattga tatttaacat cttctcctgt ctgacaagtc ggacaggaga attttttcat
    10561 ctttttttgt caagtaactt tactttacaa aaaaaatgtg ttatcctagt atggttgatg
    10621 aaaatcagta gattgaatcg aatataaata cctaaaggag aaatcaaaat ggcagtacct
    10681 gcacgtcgca cttcaaaagc gaagaaaaac aaacgtcgta cacactacaa agtaacagct
    10741 ccatctgtaa actttgacga aactactgga gattactcac gttctcaccg tgtatcactt
    10801 aaaggatact acaaaggacg taaaatcgct aaagctgcat cagctgaata atagaaggga
    10861 gataccatgc gcgtaaatat tacacttgaa cacaaagaat ctggtgaacg cttgtacctt
    10921 acttctaaaa acaaacgtaa cactccagac cgccttcaat tgaagaaata ctcaccaaaa
    10981 cttcgcaaac acgttgtgtt tacagaagtt aagtaagcat tcaatacgaa tcaatacaga
    11041 agaaaaacgc ttgatcagcg ttttttcttt ttgttaattt cactatattg caagggaaat
    11101 cattcgaatg actacatttt tcactacctt ttttgaaata agaatacgga ttcggacgat
    11161 taaaagtaag agaaactcaa atgtaagttg gggttccttt tttgtatatt tttcaaataa
    11221 cgcctctaca ctatttgtag caaattcacc aactacagtt gtatcttagt taaaataagt
    11281 tagaatatgt aagtgagtac cagatatacc aagacatcgt caccatctaa ggtatattca
    11341 aaatacaaaa gttgaccaac tagatttctg aatatcctta tatatccatt cttaaaattg
    11401 gtttaaatag cgtagtcttt taaactagtt ttgagaatcc aaaaaatctt cctacatatg
    11461 taagaagatt ttttagttca gaatgattag atttagctaa tggataccta tcctacccac
    11521 tcaccgttag cattgactcg atagccatcg ggtgtagtcg tgtttactaa taaatctcct
    11581 gaattatagg tatagtacca tttaccagaa act
//
LOCUS       DBEJAQ010000057        11337 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.057, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000057 DBEJAQ010000000
VERSION     DBEJAQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.057"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..782)
                     /locus_tag="ACL297_001800"
     CDS             complement(<1..782)
                     /locus_tag="ACL297_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGR0072010.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIH"
     gene            1379..1963
                     /locus_tag="ACL297_001801"
                     /pseudo
     CDS             1379..1963
                     /locus_tag="ACL297_001801"
                     /inference="COORDINATES: protein motif:HMM:NF021048.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            1960..3150
                     /locus_tag="ACL297_001802"
     CDS             1960..3150
                     /locus_tag="ACL297_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGR0072011.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(3665..4657)
                     /locus_tag="ACL297_001803"
     CDS             complement(3665..4657)
                     /locus_tag="ACL297_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0072012.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            4817..6571
                     /locus_tag="ACL297_001804"
     CDS             4817..6571
                     /locus_tag="ACL297_001804"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0072013.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            6555..8276
                     /locus_tag="ACL297_001805"
     CDS             6555..8276
                     /locus_tag="ACL297_001805"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0072014.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            8287..8871
                     /locus_tag="ACL297_001806"
     CDS             8287..8871
                     /locus_tag="ACL297_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGR0072015.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            8871..9566
                     /locus_tag="ACL297_001807"
     CDS             8871..9566
                     /locus_tag="ACL297_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGR0072016.1"
                     /translation="MSTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            9554..10918
                     /locus_tag="ACL297_001808"
     CDS             9554..10918
                     /locus_tag="ACL297_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0072017.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSINVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFIFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         4148 a         1604 c         1918 g         3667 t
ORIGIN      
        1 tagtgaatag acttcatacg tccttcgatt tttagactgt ccacaccatt ttcaatcata
       61 tctggaatgt ggtcaatcat agacatgtca acggctgaca ttgaaaattc ttctggaatc
      121 tcaccctgca aactcttacg ttctttccca aatggcatat cgtaaaggtc gtatttccaa
      181 cggcatgact gagaacatcc accacggttg gcatcacgca tactcatgtg gtttgaaaga
      241 gtacaacgtc cagagtatga aatacacata gctccatgga caaaggcttc aatttcaaca
      301 tctgtacgtt tgcggatctc agctaattct tccattgaaa cctcacgcgc taaaacgaca
      361 cgagtcaagc ctagctcttt ccagaactca agggtttcat agttagtggc actggcttgg
      421 gtagaaaggt ggatttcaag gcctggtgct tcagtcgctg caatcataat caaggctggg
      481 tcagatacga taactgctgc aatcccgata tcacgcagtt tacggaacca ctcaccagca
      541 ccagcttcat ttccttcgtg cataaccata ttagccgcta catagacctt ggcaccatac
      601 ttggccgcaa actgcacgcc ttcttccatc tgttcgaaag taaagtttcc cgcacggcta
      661 cgaagaccat aggcctgacc accgataaag acagcatctg ctccatactg aacagctacc
      721 tttagcttct ctaaagtccc tgcaggtgat aaaacctcag gacgttttaa tgtttttgtc
      781 atgatttctc ctatttgcaa tataaaagtt tgacattttt ccttctcatt ttatagtaaa
      841 taaaggataa aatcaagcct agacggattg ttttgacaat tctaatcttt tagactttgc
      901 ataataactg ttaaacagtc gaaaattacc ccagttttaa caagtagtca aaaagtctaa
      961 aaaagtaaaa accagtatcc caagatactg actcgtaaaa atacataaat tagtccttag
     1021 atgactactc ccactctgtt gctcctataa tggaaaagga ttgatacagt ctagaatatc
     1081 gcttttttat ttattattct gatataaaac tgttagaatt gattttttgt tttataccca
     1141 aatatgaata gagacgtagc taataagttg aataacatat ttggaaaaat gagaaatagc
     1201 aatattacta gaacttttag aaacaatcaa tatttaaaaa gaaaaaagct caaagaattt
     1261 attaaaccat gttataatag atatagctat aattttattt ataacaagag gatagaaatg
     1321 accgaattag aaagaaaaaa tcgaaaaatt agctaagaaa tattctgata acttaaacat
     1381 caaagttcaa gagagagttc gtgaaatggc aaatgataat aagagccatt atttgatata
     1441 cagagtttta ggtatttcat ttgaagaagg agaaaatatc gatttgtatc aaaataaagg
     1501 tcgtttttta tacaaatatg ctggttcatt tttagaagaa gctgcagtac tatcctttaa
     1561 cgaaaaattt ggtacagaaa atacttaaaa agttaacatt cctaattctg aaaatacaaa
     1621 acctaagact tttgaaattg attgtttagt cggagaaaaa cacgcatacg aaataaaatg
     1681 gtgggatgca actacagatg gagaccatat aactaaagaa cacactagaa taaaagttat
     1741 tcataacaaa ggatatatac caattcggtt aatgttctac tatccaaata gaactcaagc
     1801 tataaaaatt cagcaaactt tagaaacatt gtataacggt attggaggga aatattatta
     1861 tggagattct gcctgggaac atttaagagc agtgaccggt attgatttac ttagtattct
     1921 aacagatatt gcaaataaaa aaacaggggt aaaatcaaaa tgacagtatt aaaaggagat
     1981 aacttagaaa tattaaaaac tattgaatcc tcaagtattg atttaatcta tatggaccct
     2041 cctttcttta cacagaaaac ccaaaaatta tctaataaca aaaatattat gtattcattc
     2101 gaagatacgt ggacttcgat tgaggattac aaagaatttt tgtctgtaag attagaagaa
     2161 tgcaaaagag tgctaaaaaa tagtggcagt attttcgttc attgtgataa aattgcaaat
     2221 catcatatta gattaatttt agataatatc tttggagcag atatgtttca aagcgaaatt
     2281 atatggaact ataaacggtg gtctaattca aaaaagggat tattgaacaa tcatcaaaac
     2341 atttactttt attcaaagtc aaaagatttt aaatttaata caatttttac agagtattct
     2401 tctactacaa atatcgacca aatactagtg gaacgaaaac gagatggaaa ctctaaaact
     2461 atatataagg ttgataataa tggtaactat attctagcaa aagagaaaaa tggagttccc
     2521 ctttcagatg tttggaatat accatttctt aatccaaaag ctaaagaaag agtaggttat
     2581 cctacacaaa aacctattct gttattagaa caaattataa agattgctac tgataaaaat
     2641 gatatagttt tagacccgtt ctgtggaagt ggaactactt tagtagcctc caagattttg
     2701 aatagaaatt atatggggat tgatttatct gaggaagcta tcaatataac tcagcaacgt
     2761 ctggaaaatg ttataaaaac aagttcaaat ttattgaata aaggaatcga agcatataga
     2821 accaaaactg aggaagagga aaacattctt aaattattac aggcaaaaat tgttcaaaga
     2881 aataaaggaa ttgatggttt tttacctaaa cattttcaaa aaaaaccgat acctataaaa
     2941 attcaaaaaa ataatgaatg tctgaatgag agtatctctt tattacagaa tgctataaac
     3001 tccaaaaaac ttgattttgg agtagttata aaaactcatt cagataattt attatttgat
     3061 tttgatacga ttcctgaaaa tataatagta gttgaccatt ttgaacttac aatcgaaaaa
     3121 tggttaagta aaagtcaaca gttactatag tttgcaaatt atttacagaa agaacaaaaa
     3181 atgctctgaa acactgattt caaggcattt tttgttagtt taaaattact cccattcttc
     3241 ttttccaaac gtacaatata tccaaaacca ttcaaaatac tagattctat tttttataat
     3301 atcactaaat ccacctaatt ataggacgtt ttcagatttt tagtcccgtc ccagtcccag
     3361 taccggagaa atattgtttt aatataatat ctctttttgt cttctaagct cttaaaagca
     3421 aaagaacaag taaagagtca agacaaggat aaaaagtcca tattagggca aataaaaagc
     3481 tttaagacag atgacaaatc taagtcaaat aagaaagacc atagcaaagg tgcagagaga
     3541 taaatattgg cggtcttcgg actgccttta tttttttatc catttttcaa atcaaattta
     3601 ttcagactat atatgcacat atacacttaa attcatataa aaacatggct tgtaaaaaat
     3661 tactttaatc acaataatcg catttaaaat tgtgatgttt gcaagctaaa ttacggactt
     3721 cacttggaag ttttcccttg tatcttttat aatagataga aaatttgctg gcagatgaat
     3781 atccaacaga ttctgctatc tcttttatag gtagttcagt gtttaaaaga agagtttcag
     3841 ctacattcat tctttttctt tgagtgtact ctgtaatgct ttgacaatat ttttccttaa
     3901 ataaattttt taatttagta ccactcattt tagatatttt ttcaagcgtg ccttgattta
     3961 cattcgttgc aaaatgatca tctaagaatc ttgctacatc ttcaagtgct ttatcatcat
     4021 caatttcaat tttatatttt tttctattta agtatgtgtc aattactata cttatccatt
     4081 catttgcctt tgctttaaag aaaaaatcag cggcaggagc gtccatctta caatttaata
     4141 tttccattgc cactctttct aaggcctttg taagtattat ttgattcggt tgaagcaagg
     4201 ttgaataaaa agattctgga ttaatgttaa tagatgctaa atgtttttct attagctctt
     4261 ttttaaaacc aatggaaaca gcaagataac aacaattctc gtgtaataaa aaaacaaaat
     4321 catcttttat attatcaaaa tcaaaagtac ataaagagtt tgcggtaata gtttgatacg
     4381 gattaaactt ttctccgttt gcactgacaa tgtaacttga ataaattgaa acatagtctg
     4441 acatactata agtgctattt tgaactactt cctctttgat ataaaaatca tgtatatcga
     4501 taatgaagct gcctccttca taaaaccaat ataggccttc tgcataagtt gaattctctt
     4561 ttttccaaca aaatgtatga cctgcacttg aatacttctc attgttttta cattcatcta
     4621 ctttcatata atcttttaca aaatcataat atgacataac acactatccc ttttagacaa
     4681 tattccaatt agccttatta attcaaaact attgtattag taattataac agatgtataa
     4741 tagaaaagca atgatagata ttatcaatta agcgaattta tatctaaaag ggatattaaa
     4801 gaaaggagat atgcttatga agatttacaa aaaactattt gcttatgtcc aagataagaa
     4861 atatcttggg gttttggcca taattttttc tgctatatct gctctactta cagtatatgg
     4921 atattattta atctacaaat ttctagataa gttaataatt aattcaaact tatccggtgc
     4981 agagagtata gcattaaaat ctgttattac actaacaagt ggagcgatat tttattttgt
     5041 ctcaggaatg ttttcacata tcttgggatt taggcttgaa acaaatttaa gaaaaagggg
     5101 aatcgatggt ctggaaaaag caagttttag gttctttgac ttaaatccat ctggtcaaat
     5161 aagaaagatc atagatgata atgcagcaca aactcatcag gtggtagcac acatgattcc
     5221 cgatagttct caggcaataa tcacacccgt acttgtactt gcacttggct ttatagtaag
     5281 tataagggtt ggcataattt tgcttgctct tactataatt ggtggcttaa ttttaggggc
     5341 aatgatgggc gagcaagaat ttatgaagat ataccaagaa tccctatcta aactaagtgc
     5401 tgaaactgtt gagtacgtga gaggaatgca agttgtaaaa atatttaaag caaatgtaga
     5461 gtcttttaaa agcttttata aggcgataaa agattactca aagtatgctt atgattattc
     5521 cctatcttgt aaaaggcctt atgttttgta tcaatggtta ttttttggac tgattgcaat
     5581 tttaattatt cctatagttt attttatgac tagcttagct agcgcaaagg tgattttact
     5641 tgagcttatc atgattttat ttttatcagg agttctcttt gtttcattca tgagaatgat
     5701 gtggtactcc atgtatattt ctcaaggaaa ttatgcagta gatactttag aggcgcttta
     5761 cgaagatatg caaaaagaca aattagtgca tggtaatgtc aataatttta aaaactataa
     5821 tatagaattt gagaatgtta gctttgctta taatgataaa gctgtcattg aaaatttatc
     5881 ctttaattta gaagaaggaa agtcctacgc acttgtcggt tcatctggat caggcaaatc
     5941 aacagtagca aaacttatat caggttttta caatgttaat aaaggaagca taaagatagg
     6001 cgggatagca ataagtgaat attctgacga agccttaatt aaagccattt cctttgtttt
     6061 tcaagattca aaattattca agaagagcat ttatgataat gtagctttag ctaataaaga
     6121 tgcgacgaaa gatgacgtta tgagagcctt aaaattagca ggatgcgatt taatattaga
     6181 caaattccca gaaagagaaa atacaatcat aggctcaaaa ggtgtttatt tatccggtgg
     6241 agaaaaacaa agaattgcaa ttgctagagc aattttaaag gattccaaaa ttattattat
     6301 ggatgaagca tcagcatcta ttgacccaga taacgagttt gaattgcaaa aagcttttaa
     6361 aaatcttatg aaggataaaa cagttatcat gattgcacac aggctatcta caattaaaga
     6421 ccttgatgaa attattgtca tggatagtgg aaaaattata gaaagagggt ctgacaaaga
     6481 attaatgtca aaagatacaa ggtataagag cctgcaagag atgtttaaca gtgcgaatga
     6541 atggagggtt tcaaatgaaa gagttttata aaaaaagatt tgctcttaca gatggaggag
     6601 caagaaattt aagtaaagca acactggctt catttttcgt ttattgtata aacatgcttc
     6661 ctgccatatt acttatgatt tttgctcagg aagttttgga aaatatgggc aaaagcaatg
     6721 gcttttatat agtattctca gttttgactt tgatagcaat gtatattttg ctttctatcg
     6781 aatacgataa attatataac acaacctatc aagaaagtgc agatttaaga ataaggacag
     6841 cggagaattt atcaaaatta cctctatctt acttttctaa acatgacatt tccgacattt
     6901 cacaaacaat catggctgat attgaaggca tagagcatgc aatgagccac tcaataccaa
     6961 aggtgggcgg catggtactg tttttcccat taatatctgt aatgatgcta gcgggcaatg
     7021 tcaagatggg tttagctgta attattccat ctattttaag ctttatattt atacctttat
     7081 ctaaaaaata tcaggttaat ggacagaata gatattatga tgtcttaaga aaaaactcag
     7141 aaagctttca agaaaatatc gaaatgcaaa tggagattaa agcatataat ttatcgaagg
     7201 atattaaaga tgacttatat aaaaaaatgg aagatagtga gagagtacac ttaaaggcag
     7261 aagtaactac aattttaact ttgtctatat cttcaatatt tagctttata tctcttgctg
     7321 ttgtgatatt tgtcggcgta aatctaatta ttaataaaga gataaattct ctctacctta
     7381 taggatattt actagctgct atgaagataa aagactcttt agatgcatct aaagagggct
     7441 tgatggaaat attttattta tcgcccaaaa tagaaagatt aaaagaaatt caaaatcaag
     7501 atttacaaga aggcgatgac tatagcttaa aaaaatttga tattgatcta aaagatgttg
     7561 agtttgccta caataaagac gcaaaagttt taaatggtgt aagttttaaa gctaagcagg
     7621 gagaggtcac tgctttggta ggtgcaagtg gctgcggtaa aacaactatc ttgaaactta
     7681 tatcaagact ttatgattat gacaagggac aaatcttaat cgatggcaaa gatataaaag
     7741 aaatatcaac agaatctctt tttgataagg tctctatagt tttccaagat gtggttctct
     7801 ttaatcaaag cattatggaa aatattagaa tcggaaagca agatgcaagt gacgaagagg
     7861 ttaaaagagc agcaaaactt gcaaattgca cagattttat agaaaaaatg gataaaggtt
     7921 tcgatacagt tattggtgag aacggagctg agctatcagg aggagaaaga caaagattat
     7981 caatagccag agccttctta aaagatgcgc cgatattgat cttagatgag ataacagcaa
     8041 gccttgatgt taacaacgag aaaaagattc aagagtcttt aaataattta gttaaagata
     8101 aaactgttgt aatcatttca catagaatga aatccataga aaatgcagac aagatagtag
     8161 ttcttcaaaa cggaagagta gaaagcgaag gtaagcatga agagctttta caaaaatcaa
     8221 aaatttacaa aaatttaata gaaaagacaa aaatggcaga agaatttatt tattaggagg
     8281 actacaatgg ataataaaaa attaaaagta aaagatttag taagtatcgg tgtttttggc
     8341 gtaatttatt ttgccttcat gtttggagtt ggtatgatgg gcttgattcc aatattgttc
     8401 ttaatatacc cgacagtatt agccatagtt gcaggaactg ttgttatgtt atttatggct
     8461 aaggttcaaa agccatgggc actatttata tttggtatga tatcaccact tgtgatgttt
     8521 gcagctggtc atacctacgt agttgtggtt ttatcactta tagtaatgat aatagcagaa
     8581 ttaattagaa agattggtaa ttataattca tttaaataca atatgctttc ttatgcaatc
     8641 ttcagcacat ggatatgtag ctctttaatg caaatgcttt tagcaaaaga aaaatatatg
     8701 gagtggtctt tgatgacgat gggaaaagat tatgttgatg tattagaaaa gttaataact
     8761 tatcctcaca tggctttagt agccttaggt gctttcttag gaggaattct tggagcatat
     8821 ataggcaagg ctctattgaa aaaacacttt gaaaaagcag gcattgtata atgtctactt
     8881 tttcttcaaa tgctcccttt aatcccaatc cgatctctaa gatattagtc gtatttatca
     8941 caggcttaac tgttatgcat agcataaaca tccgttttga gctggcaatt gtttgtctta
     9001 ttggtatttt gctttatttg aatggataca aaaaaacact tttcaaatgg attatattgt
     9061 gtgggatact ttactccttg cctaatttta tggtgttatc tgaattaaac cctatagtta
     9121 agatgttttt gagtatacct attgttatta gaatgtttat tttaccattt atggcagcaa
     9181 gctttatgat aaagacctcg gatgtaggcg caataatttc atcgatggat aagcttaaga
     9241 tttcaaagaa tgtatccata cctattgcgg ttatgtttag attcttccca tcttttaagg
     9301 aggagaagaa aaacatcaaa atggctatga gagtaagagg gataagtttt aaaaacccag
     9361 tcaaatatct tgaatatgtt tctatgccac tactcattat atcatctaat atatcagatg
     9421 acattgcaaa agcggcagaa acaaaggcaa tagaaaatcc aattgccaag accagataca
     9481 ttcgcgtaaa gatacagcta attgattttg tttatgtttt agcggttgct ggacttattg
     9541 tgggaggctt aatatggttg aaataaaaaa tttaagtctt gattatggtg aagagcatat
     9601 attagatgat atatcactat ccatagccga gggagagtgc gtgctattta caggaaaaag
     9661 tggaaatggt aagtcatctt taataaattc aatcaatgga ctagctgtaa ggtatgataa
     9721 cgcaaagaca aagggcgaaa taattattga tggtaagaat ataaaaaatt tggaacttta
     9781 tcaaatctca atgcttgttt caactgtttt tcaaaatcct aagacatatt tttttaatgt
     9841 caatacgaca ttagaattat tattttattt ggaaaatatc ggtcttgcaa gagaagagat
     9901 ggacaggcgt ttgaaggata tgcttaagat attcccgata aaaaatcttt tgaacagaaa
     9961 tatatttaat ctatccggcg gtgaaaaaca aattctttgc attgcagctt cttatatagc
    10021 aggtacaaag attatagtta tggatgagcc ttcatcgaat ttagatatta aaagcataaa
    10081 tgttttggca aagatgctaa agatattaaa agagaaaggc ataagcataa ttgttgcaga
    10141 gcatagaatt tattatttga tggacatagt tgaccgtgta tttttaatag ataaaggaaa
    10201 gcttaaaaaa acttatacta gaagtgaatt tttaaagcta gataaaaatg aattaaatgc
    10261 tttaagttta agagataaag aattaagtaa attaaaagtt ccttatttaa aagaaggtgg
    10321 agagtatcag ataaaaaatc ttagctacaa atttactgat gatgagtgtt taagcttaaa
    10381 agatatttca ttcaagcttg ggaaaattta tggcataata ggatccaacg gacgaggaaa
    10441 atcaacgctt ttaagatgtt taataggtct tgagaaaaaa tcaaaagaag aaatttattt
    10501 taagggagag aagctatcta aaaaagaaag actcaaaaac tcttcacttg ttatgcaaga
    10561 tgtaaatcat caattattca cagatgaagt attcaacgag cttagattag gagtaaagaa
    10621 ttttgatgaa gaaaaggcga aaatcatttt aaaagattta ggcctggacg aatttattga
    10681 aaggcatccg atgagtttat caggagggca aaagcaaagg cttgcaatag catctgttat
    10741 gtgcaagaat tctccattta tcttttttga cgaaccttca agtggtatgg attattccaa
    10801 tatgataaaa atatctgaac tgattaataa gtataaaacc atggataaaa taatttttat
    10861 tgtttcccat gatatagaat ttttaaatga agtggcagat gaaatttttg aattgtaaaa
    10921 tagagtctgt taaacatgtc tatagaacta gtaaattgtc taaaaattta aaagatacta
    10981 acacatattg atatttatag aaaattaact tcaggatttt tacagtattt ataaacttta
    11041 taatatgtat ttatagtatt aataatttcc atatctttag ggaaattatt tatttactta
    11101 ccataattaa agagatgaga gataaatatc ggcagtcttc ggactgcctt tattttttta
    11161 cttaatttaa tgcagtaaat catacgattg aaaggaatct gcgttatatg ctataattag
    11221 gtagttaatc tatacattta gaaaggtaaa atatagaatt ggagaatgat atgggatttt
    11281 catataataa attgtaaccg cccaataacg aagtctattg aaaaatctcc agattag
//
LOCUS       DBEJAQ010000058        10979 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.058, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000058 DBEJAQ010000000
VERSION     DBEJAQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.058"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(178..981)
                     /locus_tag="ACL297_001809"
     CDS             complement(178..981)
                     /locus_tag="ACL297_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGR0072018.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1264..2019)
                     /locus_tag="ACL297_001810"
     CDS             complement(1264..2019)
                     /locus_tag="ACL297_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HGR0072019.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2089..4740)
                     /locus_tag="ACL297_001811"
     CDS             complement(2089..4740)
                     /locus_tag="ACL297_001811"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGR0072020.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(4752..5714)
                     /locus_tag="ACL297_001812"
     CDS             complement(4752..5714)
                     /locus_tag="ACL297_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HGR0072021.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESLKLKHSRYYISK
                     ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
     gene            complement(5716..6273)
                     /locus_tag="ACL297_001813"
     CDS             complement(5716..6273)
                     /locus_tag="ACL297_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGR0072022.1"
                     /translation="MARTELPDQIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDSLIYGLLKSEWEE"
     gene            complement(6260..6709)
                     /locus_tag="ACL297_001814"
     CDS             complement(6260..6709)
                     /locus_tag="ACL297_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HGR0072023.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(7122..7517)
                     /locus_tag="ACL297_001815"
     CDS             complement(7122..7517)
                     /locus_tag="ACL297_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGR0072024.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7613..7867)
                     /locus_tag="ACL297_001816"
     CDS             complement(7613..7867)
                     /locus_tag="ACL297_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HGR0072025.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(7877..9211)
                     /locus_tag="ACL297_001817"
     CDS             complement(7877..9211)
                     /locus_tag="ACL297_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HGR0072026.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9211..9444)
                     /locus_tag="ACL297_001818"
     CDS             complement(9211..9444)
                     /locus_tag="ACL297_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HGR0072027.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(9860..10042)
                     /locus_tag="ACL297_001819"
     CDS             complement(9860..10042)
                     /locus_tag="ACL297_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072028.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(10044..10823)
                     /locus_tag="ACL297_001820"
     CDS             complement(10044..10823)
                     /locus_tag="ACL297_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072029.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT         3218 a         2440 c         1978 g         3343 t
ORIGIN      
        1 cggattttta tcgctctgaa catcaaaaaa gaaaggacga aatttgtcct ttctcgaact
       61 tagcttttct tcaacccact acaattgaca aagagccaaa ctttgatgat atacgttttc
      121 ttgtgagaat atttacttca ttttttccta aaattcaatg tttactcagt atttggatta
      181 tgaaaaatcg aggtctaaat ctagatacat tttttctgaa gacaaatcat tttgaccacc
      241 gagcaagaga ttttcaaaaa aagctgttaa aaactcagga cgtcgctgta aaatctttgc
      301 attatctaat accaaggcat cacgaaaata cttggaatgt tgctgaaatg gtatattatc
      361 aatatcaaaa ccaaactcac gaagatactg aatcaaaaag accgttactg tccgagtgtt
      421 tccttcgcga aatggatgaa tctgccagat tcctgaaata aaatgctgga tttgtttaac
      481 cacatccgcc tgagttagtg tcgcatatgc aacttgtttt tcctgattaa aatcataatc
      541 taaggtcatt tgaatcatgg agtaatcaga gtacacaaca ctttcaccat tcaaaacagg
      601 ttcattcttt gtgatattgg tctgacgaaa ttgccccacc ggaatagagg gttcaaatat
      661 atcttgaaac aactccttat gaatagcaag taaggtcgca ggactaaagc taaagcctct
      721 tcgagacaat agttctacaa tacgtagaga aaccaagtct gcctcttccg tacttgcatc
      781 aatagtatgg tgataagccg ttgcatcctc ataaacctgc tcataagtca gttctccccg
      841 ggactgtttc tcagccaaag attccatata cgctgatggc actagattgt caactttctg
      901 cagaccaaaa cctatccgcc ataaatcacg cttcgcttca taagacaagt ttggattgtc
      961 aatgttgtaa gttggttgca tagaaatatc ctttcaattg tttttcttca ttaccagtaa
     1021 atcacgaaat tggttggaaa ctggcttaaa ctataaaaag tcctcacaat aggttctatg
     1081 atatatatat atatatatac ttcatgtaaa taataaaaga gttataatca gcactactga
     1141 taaattcaat accgatttcg ccatcttaaa acttagtcaa acgaataccg ttgatttcca
     1201 gttcattaag tcgttgacct gtcaagactt ctcgttcctt caactggaaa agttaagggg
     1261 gaactaatac aaatcctcta cgcttctata ttcaatgatt tcatttttga taatattctt
     1321 tttaaactca aaatgtttta aaggattatc gattaacact aactttggaa tatcatctag
     1381 atttgtgacc aaggataaaa taaaatccga ttgaaactcc ttgacttttt caaattcttt
     1441 tgcagttaaa cgcacacgcc ccttagattt tcggatacct tttacttcgg caaggtaaga
     1501 atactcttgg acatctacct gaaagtcata tccatcacca taaagacggg catcggttaa
     1561 ctgtccacct tggaattttt cttctttatc aaaatgaagg ataaaataat tctcagcctc
     1621 cattcccgtt tcctgtagac gtttgaattt agttcgaact attggttttt caacgatggt
     1681 tatgcctgtt ttcttccctt cactggcaag taacatttta actatttcag cataactatg
     1741 aacatcttca tttccaaaca tcatatcaat taaatcttta cgaaaacgat aaacttcagc
     1801 tttctgccac caaccttttc gattattgtc aaaataagga tcaaataaat ccattctatt
     1861 ttttacaact ccagttgttt cagcaattcc cagagaaacc acatagcgat aaaactccga
     1921 cttgctaaag cattgaaatt cttttataaa aagcttgtca aacttggcaa gaccgtagcc
     1981 aattaaattt aacaattcat aatgagggtg tttggacatc actttctcca tcaatagata
     2041 ttgatttcat tataccaaaa aacacggctc atcaccgtgt ttctgtgttt atttcaccaa
     2101 cttcttcatc tcatcaatac gtgctacggt cacatcatac ttggcttggt agtcggcttg
     2161 tttgtctttt tctttttgga caacttctgg tttagcgttg gcgacgaagc gttcgttaga
     2221 gagtttctta ccaaccatgt ccagttcttt ttgccattta gcgagttctt tgtcgagacg
     2281 agctagttct tcttcaacat tgaggagatc ggccagtggc aagaagattt cggctcctgt
     2341 aataacgctt gacatagcca gttcaggtgc agggatggtt gatgcgattt ccaagtgttc
     2401 tggatttgta aagcgtttga tatagttgac attgctgtta aagaaggctt ccaagtcact
     2461 atcgcttgtc ttaacaagga tggtgatagg cttgcttggt gctacattta cttccgcacg
     2521 cgcattccga acagcacgaa ttaagtcttt gagactttcc acaccagtgt gagccgcaag
     2581 gtcttcaaag gctagattaa cagttgggta tgcagctgtc acgatagaac cttctgagat
     2641 ttgtccaaag atttcctctg tcacgaatgg catgattggg tgaaggagac gaaggatctt
     2701 gtccaaagtg tatagtagaa cagaacgtgt gatgactttc tcttcttcgt tatcgctata
     2761 aaggacttcc ttggtcaact caacatacca gttggcaaat tcttcccaaa tgaagttgta
     2821 aaggatatga ccagccacac caaactcgaa cttatcaaag ttttcagtaa cttttgcaat
     2881 ggtttcgttg agattgtgga gaatccagcg gtccgtcaca ttaccagcct cacctgttgc
     2941 aacttttgtg acattgtcat gcgccacatc cagcgtcaaa cctccattgt tcatgaggat
     3001 atagcgagaa atgttccaaa tcttgttaat aaagttccat gaagcatcca ttttctcata
     3061 agagaaacgc acgtcttgtc ctggcgcaga accgtttgaa aggaaccatc gaagggcatc
     3121 ggcaccgtat ttctcgataa catccattgg gtcaatcccg ttaccgagag acttagacat
     3181 cttgcgtcct tgctcgtcac gaatgagacc gtggataagg acgttttgga atggttgacg
     3241 gcctgtgaat tccaatgact ggaagatcat acgagacacc cagaagaaga tgatgtcgta
     3301 acctgttacc aaggttgaag ttgggaaata acgtttaaag tcttctgagt cgacttcagg
     3361 ccagcccatg gttgaaaatg gccagagggc agaactgaac caagtatcca agacgtcttc
     3421 gtcctgagtc catccgtcac cttctggagc ttcttcgccg acatacattt caccatcagc
     3481 attgtaccag gcagggattt ggtgacccca ccaaagctga cgagagataa cccagtcgtg
     3541 gacattttcc atccattgaa ggaaggtatc gttgaaacga ggtgggtaga attcgacctt
     3601 gtcctctgtg tcttggttgg caatggcgtt cttagccaat tggtccatct tgacgaacca
     3661 ttgagtagac aagcgtggct caactacgac acctgtccgt tctgagtgac cgactgaatg
     3721 gacacgtttt tcgattttga caagggcacc gatttcttcc aacttagcaa cgactgcctt
     3781 acgagcttca aaacgatcca tgcctgaaaa ttcaaaggca agctcattca tagttccgtc
     3841 gtcgttcatg acgttgactt gtggcaagtt atgacgttgg ccaaccaaga agtcatttgg
     3901 atcgtgggca ggtgtgattt tcacgacacc agtaccaaac tcaggatctg cgtgctcatc
     3961 tccaacgatt gggatgagtt tattagcgat tggaaggatg acgtttttac caatcaagtc
     4021 cttgtagcgc gggtcttctg gattaaccgc aaccgcaacg tccccaaaca tagtctcagg
     4081 acgagttgta gcaacttcaa gggcgcgtga accatcttcc agcatgtaat tcatgtggta
     4141 gaaggcacct tctacatcct tgtgaatcac ctcaatatca gaaagggctg tgcgagctgc
     4201 tgggtcccag ttgatgataa actcaccacg atagatccag cctttcttgt aaaggtccac
     4261 aaagacctta cgaacagctt ttgacaaacc ttcgtcaaga gtgaaacgct cacgagaata
     4321 gtctacagaa agccccatct tgccccattg ttccttgatg gtagtggcat attcgtcttt
     4381 ccattcccag accttcgtca agaaagactc acgacctagg tcataacgcg taataccctc
     4441 accacgtaag cgctcctcaa ccttagcctg agtcgcaata ccagcgtggt ccatacctgg
     4501 aagccaaagg gtatcaaagc cctgcatgcg tttttgacgg atgatgatat cctgcaaagt
     4561 cgtatcccaa gcgtgaccaa ggtgaagttt cccagttacg tttggtggtg gaatcacgat
     4621 tgaataaggc ttagcctttt gatcgcctga aggcttgaaa acatccgcat caagccattt
     4681 ttggtaacga ccagcctcaa cctcggctgg attgtattta gatgaaagtt ctttagacat
     4741 gtgtgttctc cttaattact tttattgaca attttaacca aaccaatttt tgaaagttca
     4801 atataaatct cttcaatttc agcaaatgct gcaagtggtg acatagtata atttccagga
     4861 ttatatttaa gatcatactt gcttatcttt gaaatataat acctagagtg tttcaatttt
     4921 aatgattcca actttgattt aatatctact aatgtattat ggtttaactt taaaaacaac
     4981 ttattataat taccatagag ctctgtaaga gcattaaagt attctttcac tagagctata
     5041 gcgtcaatct tagatgtttc ttggtttagt tttatcaact cccctttcaa ttttttccat
     5101 ttaaagttgg aatttaaaag ataatctaaa tcaatataga aatctattat aacagtctca
     5161 tcatctgggc taacagcttt tacctcattt attggtaagt cagtatgttg cgaataattt
     5221 ctcaaagaat ggcaaaatcg ataagcaaaa ttggtatcat actgatagct tgcaaaacca
     5281 tgtatacaat caaactcctc actatctttg gaatattttt gtttaatttg gttttcgtta
     5341 aaatcgacaa acaattttcc actggataat aaatgaatca tgaaacgatt tacatctgcc
     5401 tcttcaattt ctaattcctt tgactcaagt ttatttttaa tacttaaaaa atccgagaca
     5461 tctgaaataa acaattcgaa catattcttg attttttgaa aattcttttg aatctcaaaa
     5521 atttggtccg agactgacat tatttccttg gttggttcaa tatcagagat gacaatacgt
     5581 ttttcattgt tgttaatttc taaataagct aatcgctcga taatatcact atcatttaat
     5641 tcagcatact caacatcaat ttggcgtaac tgcttctcag atagagagct tacttcctct
     5701 tttacatcgc tcatcttatt cctcccactc gctcttcagc aagccatata tcagactgtc
     5761 acagcagttg ccttgagcat ctttgcggtc tcttatgcga gcttcgaggg taaagccaag
     5821 cttttccgcg actcgtttac tttgaaggtt atatccaaag caagttagtt caatcttgtg
     5881 aagacccaaa tctttaaagg ctaagtcaat caaggcacgc gcagcttctg gcacataacc
     5941 tcgcccccaa tagtctgggt gtaaggtata gccgatttcc agcacatcat cttcatggcg
     6001 atggttgaaa tcgacagagc caacgatctt atccgttcct ttgacgacaa tcccgtagcc
     6061 tgctgggaga ttttcctttt gattacgctc cggaagaatg tgctccagat aataaatctc
     6121 atcttccaag gtcttgactg gaggaaaacc tgctggatag gcgacctctg gcaaactagc
     6181 atagtcaaag atatcctcag catccgccac agtacggact cgtaaaacga gacgttctgt
     6241 ttcgatttga tctggcagct cagttcttgc catccttctt cctcgctttt ttgatgaaac
     6301 tgcccttcat atctacacgc ttatccagat agcgataaac gcgctgatat ccatctccca
     6361 tgaaataggt tggggcaaac agttgatttt taaaatgtcc cttttcatcc aggagttctg
     6421 gggcaacaag tcgctcaaga atcttggcaa agatgtggca aataccgtct tcctcaacaa
     6481 tcctatctac cctacaatct aaaacaagtg gacaggcgtc taaaatagga gtctgagttc
     6541 gttcagaaat ttcataatgc actcccaaac gttccaattt ctcctgatga ctgataaaac
     6601 cagcctgctc catcgcaagc atagaagttt catcagaaat attcacagta aatttttgat
     6661 actgtttgat ctgctctgcg gcattctctc tcgcaacgac tccaatcaca acccaatctc
     6721 ctagactata agaggaacta caggtcgtga tgttatagcc aaaattctaa tcttgatatc
     6781 ctaaaataaa aacaggaaaa ccataatata gtttacttgt gttaaaagat tgcttcataa
     6841 caaccccctt tgactaagac gtaaaagaaa agccctgcca tctacatgac agggacgaat
     6901 gtgtttatcc gcggtaccac ccaatttcgg gcagttgccc gcaactctcg tctttaaagc
     6961 aaaaagacac ttttatttca ttttttcagt cattagcaac cagttttgac acatttgtgg
     7021 acttctcagc gccgccactt tctgtaaaat gtgggattca aaacacctct aatggctcta
     7081 ttgtagcaag tttttctcgg aaatgcaagg ctcaagaaaa attacttgaa cttgatgcca
     7141 ttttctttca atttcttgat ggtagctggg ccgattcctt tcaaggcaag gagttctttt
     7201 tcagtccagt ttttgaaatc tgaagcagac ttgattcctt catcatagaa agtcttggca
     7261 cgatcaagtg gaagtccacc caagtttgct gcaaaatctt ctacagaatt agctactttt
     7321 tgagcttgct ctttggtagc ttctactgct tgttcaactt ttttagaaac agccttacca
     7381 gctgcgcttg ctgactgttc tgcacgcttc acgacttttt ttgtctcatc tacaacctta
     7441 gtcacagtac ttgaaaaggc acctgcgcga cggaggccat ttcgtaattg ttttttacga
     7501 ttgagtttct ttgacatgat aaaatccttt caataaaaaa tccctgctct gacaaggatt
     7561 taacataaac attctatcaa gattttagtg aaaaatcaag aatctttctc gtttaataat
     7621 ttcgctcacc aaacaagcct tctggcaaat catggaatcc cttgcctgct ttgaagtttt
     7681 caaacttacc aagcaagaac tgataagcat ccaagcgact ctcgtagcga atcatggcat
     7741 ctttggcacg ctcgtcctgg tcttcttcgt aaactttttg actttccttg tgcgccatgt
     7801 cgttaatcat gatttgggta ttgacccaag cttcaaattc cttcataaat tcttgttcgt
     7861 aactcatttt ttctccttat tctaatccac gaaaatagtc cgtatcaatc tcacggtagg
     7921 gctggatatc ctgaacatac tccaacactc cttggaattc tccgtcttca tcgtgtactg
     7981 cagcataggt gatgtggaca aacttacctc gcgactcaga cttgaaccac atttcatact
     8041 tgtccttggt cccctcacga agccccttca tgatagtttt gaccttgtcc aagtacttag
     8101 gcggatggca gagttcgaca ttgcgcccga cttgagacgg cgtccgtttg aaaatcatct
     8161 catcagctgg cgtattgtca ttgtaatact ggaaaatatc atctttattg acaaaggtaa
     8221 tctccatagg gaggtgattg aggatgagat tggcctgctc gactgaaaga tagccattac
     8281 caaaagcctg ttgactatgg cggtccagca cagcttcctt ttccttaggg gtaaaggtaa
     8341 tggtaaaatg gccttctggc gtatctatga cttgttgaac ttgaccttct gccgtatcta
     8401 gctgtacagg ctcctctgca atcttttcct caataaagct ctgtcgttct ggcacccatt
     8461 tctctgacgg acggatgatg gcatagccat aggcatcgct ctcctccgca atctgaagcc
     8521 agtcatcctg agtaaaagac tcaaggagaa tcatgaggag gatggactct tccttgaaaa
     8581 tcatactttc aaactctgtc gcaaaagctt caaaagcttc ctttacactg ctaattgaca
     8641 cttctggtag tgacttggct atcgttagag ctgtttgaaa gagttcccta atctgatcat
     8701 ccactcccca cataactttg ggaggtgaat cgtgtccata gcgctccatg ataggaaaga
     8761 agagttcttc cttacgttgg taatggatgt caaattgacc cacaagtccc atctgacgca
     8821 ccaaaccctt acgcatctcc gccagcattt cctcgtcttc catagactca taggtatcta
     8881 acaatctacg aatgcgaatc aaggccgcac ggagagccag attttcttct ttgaagacac
     8941 gaactgggtg acctggatgc tcagtatctg aaacttcgac acctttgata gcatttttaa
     9001 aaagattggc atggacatca cagagttcca taacatcttc aaaggtgaca cctgagtctg
     9061 agttcatcag ctcgtgctcc ataagggaaa tctcaatggc tgagacaccc gcaaaggtcg
     9121 catcaaaacg ctcctgaacc gactcaggag aggcgccatt atgcaattct aataaaatat
     9181 cccgtaggac atgaatccgt tcatctgcca ttagtctaat ccaatcactt cgtagccatt
     9241 cgcttccagt gtgcgtacaa tcttgtccat aggagttcct gctagcttag aaccttgttt
     9301 aagtgatact ttacgaccaa ctgtattgcg catcaaggga ttggcaaggg gtttaaaacc
     9361 cagctccact agaatttcca agacttctgg atgcttgtcc accacttctg caacaggaat
     9421 tgacacatcg atgatattgt ccatgacgac ctccattata tgttctaata ctcaatgaaa
     9481 atcaaagagc aaactagaaa gctagccgca ggttgctcaa aacaccgttt tgaggttgtg
     9541 gatagaactg acgaagtcag taacatatat actgcaaggt gaagctgacg tggtttgaag
     9601 agattttcga agagtataaa atgattttac tgcttatatt ataccatatg gagagagatt
     9661 aaaaaactag cagtggagtt cctgctagct ctctgcgact tggattacca tctagataag
     9721 tttaaagagc caatccaata tcttaaataa tatcttgtaa aacagcaatt gaactgcaaa
     9781 ggaaatgatc atccaaaaga atttcacaat cccaataatc ggtttgataa aaagaatagc
     9841 aagaagacca aaaaaaattt cattctctct cctctggcct aatgagccac cgagccgtat
     9901 ccattagctt atctgcaata aaaagttttc ccagaccaac aacgacatgg tcagctttct
     9961 ttatcgtttt catcactttt acaccttgca cggtcaaaaa gtaattacca taaattttat
    10021 caaaagcttt gataggattc atattactct cctttaatga tttcagcctc ttttcggatt
    10081 gtttcaacat cttcttgata ttgaacttca ctatagttga ctagcttgga taattctttc
    10141 tgcaagcttt cccccattgg tttcatagtt gcaaaggtta aaccacctgc aatgatacca
    10201 cccaagatag gaataaattt ccctattcct ttggccagcc ctcccttggt aagattcaca
    10261 ccaaatattt ttaagacttt tttcaaaata gggtaccaaa gcgtctttgt taaagcttta
    10321 ttaggcactg ttttcattac ctgtttggca attgttatac caccagcaca tagcaaagca
    10381 gcggttccat tcacccctaa cattacgcct agataaagca agagggtatt ttgagcatct
    10441 tcactcaact cctcgcgtga agcccaaaga tcctcataac cataaatata acctaattct
    10501 tgagccaatt tcagagagaa agcataaaat tgagccacat cagctggaat ggtaattgcc
    10561 atagcaagcc ctccagctaa tcctgccaaa acagaagtcc cactcgctaa taatacattg
    10621 tcccgaatac aagcattagc tacacgatct aatatttctt gagatagaag agtcgttgta
    10681 ccttgttcca ataaggtagg aatatctttt ggatccaatt ctttggaaaa cttatccact
    10741 aaaaatttcg aacgatcaac cttaacaaca ggtagtttca ccacttgttg caatacttgc
    10801 atagctaagt cttgttcagc catatacaaa ctccttattt cttttttaca ccttatcata
    10861 ttatatttcc tacaaaaaaa gcattttcta attagataac attaaaaaat acagaacccc
    10921 ttttttatag taaaatgaaa caagaacagg acaaatcgat caggacagtc aaatcgatt
//
LOCUS       DBEJAQ010000059        10796 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.059, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000059 DBEJAQ010000000
VERSION     DBEJAQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10796
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.059"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(155..1579)
                     /locus_tag="ACL297_001821"
     CDS             complement(155..1579)
                     /locus_tag="ACL297_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HGR0072030.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2124..4403)
                     /gene="pulA"
                     /locus_tag="ACL297_001822"
     CDS             complement(2124..4403)
                     /gene="pulA"
                     /locus_tag="ACL297_001822"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HGR0072031.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4515..6473)
                     /gene="ligA"
                     /locus_tag="ACL297_001823"
     CDS             complement(4515..6473)
                     /gene="ligA"
                     /locus_tag="ACL297_001823"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGR0072032.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6566..7744)
                     /locus_tag="ACL297_001824"
     CDS             complement(6566..7744)
                     /locus_tag="ACL297_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGR0072033.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7824..8687)
                     /locus_tag="ACL297_001825"
     CDS             complement(7824..8687)
                     /locus_tag="ACL297_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGR0072034.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8690..10591)
                     /locus_tag="ACL297_001826"
     CDS             complement(8690..10591)
                     /locus_tag="ACL297_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGR0072035.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3249 a         2372 c         1918 g         3257 t
ORIGIN      
        1 agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattctg gtttcaatct
       61 attataatac caaaaagttc gtattttttc tataattatt attattttat agcaaaaaaa
      121 taaaaccagg aaaacaattt cctggtttta cacgttattt cacatcaaaa acaatggatt
      181 tgacatttgt catcgcttca atgctatatt taattccttg cactccagct ccagaacctt
      241 tgacaccaag gaatgggaaa ttatctggac cacgctgggt tttattatta atgtggactg
      301 tacctacttc aagtttttca gcaatttcaa aggctttttt gaaatcattt gtaaagactg
      361 atgattgaag gccgaattca gattcgttgg caaaggcaat agcttcctct acactagcca
      421 cacgaatgat tggtaaaaca ggaccaaatg gctcttccca tgccactttc atatcttttg
      481 taacttggtc aaaaagcact ggccagagaa gattgccctc acgtttgatt ggtgtaagag
      541 cctgagcttc tttttcttgt gcatcctcaa tcaagcccca aatgaagtcg gctgaagcat
      601 tgtcaataac aggtgtaata tcagcattgt caaatggatc accaactgtt aatttagaaa
      661 cttcttcctg aagcaaagta gctaatttat ctgctacact ttcgagaaca atgacacgtt
      721 taatggccgt gcaacgttgt cctgagtagc taaaggctcc cgcaacaatt tgcttggcag
      781 catgttccaa atctgcatct tctagtacaa gagctgcatc tttcccacca agttccaaca
      841 tgataggacg cataccagct aaacgaccaa tacgttctcc aataggagtt gaacctgtaa
      901 agttgatgaa gttgacttct ttgtgctcaa tgatataatc cccaatttct gaaccacgac
      961 ctgtaatggt gttgaaaaca cctgccggaa tccctgcttc ttcaaatgct ttagccaaca
     1021 agagtccaga aatggaacct tgtgttggtg gcttaaacat gaccacattc cctgcaatca
     1081 aggcaggtgc aattttagaa gcagataaat taactggata attaaaggga gcaatcgcta
     1141 gcacgatacc aactggttca cgacggacaa cagccagttt gtttttactt gctgcctcaa
     1201 aaccaccacc ttccattgct tgtccagtga tacggagacc ttcctcagca gcataacgaa
     1261 tcaagtctgc tgtacgcact acttctccaa ttgctgcttt aatccctttt gctacttctt
     1321 tggcaaggat agtaccaatt tcttccttat cgcgttctaa aatagctgct gttttatgta
     1381 aataagccgc acgttcaact gctgataaag ctcgccatgc tggcagggct gcacgcgcag
     1441 cttgcatagc ctcatcagct tcagtctgag tcatggctgg aactgtaccc aattcttctt
     1501 gattgattgg tgaataaatc gtaatttctt gttcagatga tttccatttt ccatttacta
     1561 aattctgata tcttgtcaaa ttcttgtcct cctgaaaatg attaagatat attttaccaa
     1621 attttcagaa aatcacaacc ctttttgcaa aatttttgag attattttca caaacttgat
     1681 ttttcaaagt atactcaata aaaataaaaa aaatccacta cgtcaaggcg aggctaatgt
     1741 ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa taaaaaaact
     1801 gaacaatttg gttggggaca gccaaaccaa tttctcacaa tgtttcagaa acaagggtgt
     1861 gctactccaa tttcagccta ctataactgt catagattgc tgaaacaaag tctaggtaaa
     1921 agtcttcata ataaaaagac ctcctatcaa gtgttcaaaa actttgatag gaggtcttgt
     1981 tttgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg ttctttctat
     2041 tctttcaatt tcaatgaatg gattttttac taatactcat aactgggaat ttgtctgtgt
     2101 aaaaatagcg agatagatgg tatttataaa acactcaaga cagctagact aatatcattt
     2161 aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt tcctgcattt
     2221 tcaaattgat agggttctga tttagcattc acaaccacca agaggtgttc tttgccgtga
     2281 acttcataga taaggtagcc gctatgttca atcgcagaat gcacaaagac atgatggtaa
     2341 atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg gatgacttga
     2401 cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac ttggttcaca
     2461 ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt caactcacca
     2521 ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc cataaaggcc
     2581 atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat gatttgctct
     2641 gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt gtcatgggct
     2701 tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg acttcctagg
     2761 attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga cttgatagca
     2821 ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa accaatattt
     2881 ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc tgttcccata
     2941 tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct ttgacgaatc
     3001 atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc gtcaatatta
     3061 tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat ttcgtgttca
     3121 ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg attcatacga
     3181 taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa ggtatggtta
     3241 tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac catcaccttc
     3301 aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt ttctggcgcg
     3361 ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc atcgtattct
     3421 ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt cttgatgtaa
     3481 tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc acccaagaaa
     3541 gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc acgaatatgc
     3601 atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc cgaaccgtgc
     3661 ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga acgtttgcca
     3721 tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg agggaattgg
     3781 acttgatact gataagcctt acctaccaaa tcttcctcaa catccaaact ccagacaccg
     3841 attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc aaaagtcttc
     3901 caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc tgtcgctgta
     3961 ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacccaa ttctccttgg
     4021 taaccccaat gatggtcaaa actagcactg ttaatggcct tatcaaaggc aaaaggattt
     4081 tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat cctatcatcg
     4141 ccttccaaaa tccagacctc tgttaatagg ggatagtgat taaaacggat agaatattct
     4201 ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg tgaacttggg
     4261 tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa atcaattcgt
     4321 tgatcctgac tttttacaaa ggagcaagtg tcatattctc cattcttacg atggtaatga
     4381 atgcgcatag ggtagttata cattttttat ttttcctttt tactttgttt ctatttcact
     4441 aataaatttt tgtcaatctc gtctcaatta acagacatag tcatattctc taaactctgt
     4501 ttttaaacga tccattacaa actttctagc catgcctcat ctctgacctg gataccaagt
     4561 tcttgtgctt tttgcagttt acttccagcg tctgcaccta ccacgacgag gtcggtcttt
     4621 ttagaaatac tacctgtcac tttggcaccc agactttcga gtttactttt agcttctgag
     4681 cgcttgagtc gttccaattt tcctgtcaat accacggtca aacctgacaa ggccgcatcc
     4741 gctactaccg tctgtccttt atagtccaga ttgaccccag tttctttcaa ttctctgagc
     4801 agaatttcag agccttctgt cgcaaaataa gtctgaagac ttttggcaat cacgccacct
     4861 agactttcaa tactagccac ttcctctgaa tctgcctgag acagattttc aattgaatgg
     4921 aaatattgaa gtaaaagctg actaaccttg cttccgacat gacgaattcc caaaccaaat
     4981 aagagcttct cggcagaatt ttcctttgat gcttggatag cctgatacag tttagcagcg
     5041 gacttttcct taaccccctc taaaaggagg aaatcctctt cttgcaaacg ataaatatcc
     5101 gccacatcct tgactaaatt agcagcaaaa agcttctcaa caatagatgg accaaggcct
     5161 gtaatattca tagcatcacg agaagcaaag tgaatcaagc cttccatgat ttgagcaggg
     5221 caacgcggat tgatacaacg tagggccact tcatcttcaa agtgcaacaa gtcagagtta
     5281 caacttggac agtttgtagg gatatctagt ttttcttcag aaacccgttt ggactctacc
     5341 acacgtaaaa cggcagggat gatgtcacca gccttatata caatgaccgt atcgtctttt
     5401 cggatatctt tttcagcaat ataatctaca ttgtgcaggg tcgcacggct aacagtcgta
     5461 ccggcaagtt gtactggtgt tagattagca gttggagtta caacaccggt acggccaact
     5521 gtccagtcaa ctgataagag ttgagcttct ttttcttcgg cagggaactt gtaggctact
     5581 gcccactttg gagccttaac tgtaaaacca agttcttctt gacttgctag gtcgttgacc
     5641 ttgattacca ctccatcaat atcgtaaggc agattttccc gttcctgtcc tacttcttgg
     5701 ataaaattcc agatttcatc tatgttttca gccaagattc gcttaggatt gaccacaaaa
     5761 cctagttgtt ctaggtactt caaacccttt tcttggctat cacgagttga agggctggct
     5821 tcttgataga gaaacgttgc aagattacgc ttggcaacta ctgctgtatc caactgacgc
     5881 agagttcctg ctgccgcatt acgaggatta gcaaattcag gctctccatt ttcttggcgc
     5941 acttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac attccccacg aactgtgata
     6001 tctagttctt ctggcaaagt caaagggatg tccttaacac gcttgaggtt ttctgtgata
     6061 ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa ccaaaatccc cttttcataa
     6121 gtcagcgaga tagataagcc atcgattttc agctcacaaa tataggtcgg atgagccact
     6181 tccttacgaa cacgcgcatc aaaagcatct agctcctcac gtgaaaaagc atcctgcaaa
     6241 ctataaagag gatactgatg actgtatttt tcaaaaccat ctaaaacctt gccaccaaca
     6301 cgatgagtcg gactgtctgc tagcacttgc tctggataag cagtttctaa ctcgaccaac
     6361 tcacggtaaa ggcggtcata ctcactgtct gcaaccgagg gattatcgct ggtatagtac
     6421 tcagtcgcat agcgattgag caaagcgact aactcattca ttcttttatt cataagacca
     6481 ttttaccata aaacaagccc tcctcacaaa cgagaagggc ggaaaaaaca cttagtttga
     6541 aattattttt gaaactcaag caaccttata tcaatttttc aaaatgagtt cgaacataaa
     6601 taaacgatat acaagacaag atgataacac cacttccaat tatcaggaaa gaagagagat
     6661 gtacacttgg caagactgtc ataaatcctt ttgcaatagg cataaataga atagctaagg
     6721 taaaaattgt actcagtact cttccaagaa attcgctctc aaccttggtt tgtacttgag
     6781 taaaaaagtg aatattaaaa atcgtcataa acaattcaca aactaaattt ccagaaaagg
     6841 aaagaaaagt tggaagtggt aatcccatca taaaaactcc gacacctgtc aaagccagta
     6901 aaatcaaaag attataaata ttagctttaa ttttactagc tagaagagcc ccaatgatgg
     6961 aaccaatagc ccccatagtt aaaatacttg cataggctcc ttctgacccg taaagctgat
     7021 tcgaaaaggg aagtagaaat tcaaaagctg caaaaaagaa attaacgctg gaagctacca
     7081 gcaaaaggaa gaaaatttct tgctgatgcc agatatagtg taacccatcc ttgatatcta
     7141 caaaaatatc tctcccagta aaagcctttt tctcttgaac ttttgcttcc tcttttggaa
     7201 ggaaagccac tagaacaaaa gcaatgaaaa aagtcagcga gtctagcagt agcgtcatat
     7261 tgagacttgc aaactgtaaa acaaggaagg aaagaacagg agagctaaca cctacaacct
     7321 gcaaaaccag ctctaagcga gaattataga tcacaatctc atctttctcc accacttcag
     7381 ttatgatagc tttattggct gtgcgagaaa aggcaaaagc aatagcctgc acaatgttag
     7441 caacaatcaa agcgccaatc atccagctat cattccttat gaaagaaata gccagacaaa
     7501 gaatcccaca aacaagatct gccgtcatta aaatcttacg acgagaaaaa cggtctgaaa
     7561 taactccgcc aaagggattg acgagaatag atgtgacgag ctcagaaatc tgatacattc
     7621 ctaaaactgt ctgtcctata gtccccatag aagccaacca gacactattt ccataatcat
     7681 agagcatatt tcccatttta ttgatagccc cacggctaat caactgcact gcatagcgat
     7741 tcatattaaa gctcctctca aattttgaaa ctattgtatc aaaaccgaaa ggagcttttt
     7801 attttttccc ttatttggga aaattaactt ttgacaaatt tttcgtagtg ttcctgataa
     7861 taggctactt gctctggaag acctaacaca tcaaaaatat gcatggcctt ttgcatctgc
     7921 ttacagcctt ctttacactg tcctttttga tataaggcaa aaccttttaa ataatggaaa
     7981 acattacgct cataaagctt aatacctttg tcaataatct tctctgtata agcctcaaaa
     8041 tagttggcat tataaaaaga agaatgctct aaacaatgct ggtaacaatt gagggccaaa
     8101 atcaaaacta atctcttatg gcgactaatc tcttggtaaa attcctccct ctccataact
     8161 tctctaccaa tccgagtgac atagtctaca tcgtagaaac tatagaggtt accgaaaaga
     8221 atcaactcat acatggtcca ttcttctgtt ttgaagagat aatctgctac cttatccaaa
     8281 tcatcctgct tcatatcata agtcgcatct ctttgacaaa tcagaccttg tagcaaaatc
     8341 cagttcagct caaaataaag ggaagtcgtc gaactcttag acttttcaag ttgttctctt
     8401 tgaagctttt gaaaacctgc aatatcgttt gaatagtaaa gtgggataat ctgtgccatc
     8461 atagacacat gttcatgatt atgaaaattc cttgccttat ccatgaaatt ttcgattgtt
     8521 acatgaatgt tatccaaaat ctcaaagaaa cgggagactg ccaggtcaga ctccccaagc
     8581 tcaaagcgag ataactgaga ggtagagcag gattcgcctg ctgcttcctt taaagaataa
     8641 tttccacttg ttcgaaattc acgaaatact tttccaagat gttccatctt tacacctgat
     8701 ctgataattc ttcccactca agcatagctt cttcctgacg atggctgatt ttgtccagct
     8761 cagcctgtaa ttccatgagt ttgtcggcat cgtttgtttc caacatttgt tcagaaatgg
     8821 cttggctttg actttctagc tcttcaattt cagcttctag actttcgatt tgtcgcatga
     8881 gtttgcgaac ttctttttga ctttctttct gggcctgata gtcattgact ggacttgctt
     8941 cctttgcttg attgctagtt gaagcttcct cagtctgact catttctgct gttgctttct
     9001 tctcaacata gtagtcgtaa tctccaaggt agagagttga accattctca gacaattcca
     9061 aaacatgagt tgccacacga ttgataaagt aacgatcatg actgacaaaa agcaaggtac
     9121 catcaaagtc aatcaaggca ttttctagca cttccttact atcaatatcc aagtggttgg
     9181 tcggctcatc cagaatcaaa aagttattgt tttccataga caatttagct aaaagcaaac
     9241 gagctttttc gccaccagat agcatgccga ctgatttttt aacatcatct cctgagaaaa
     9301 ggaaggcccc aagacggttg cggatttcaa cttctggtgt cagtttgaaa tcattccaga
     9361 gttcatccag caccgtatta cttggtgtca gcttgctttg ggtttggtca tagtaaccaa
     9421 cctcaacatt agcgccaaag cgcttttcac ctttgataaa aggaatctgg tccacaatag
     9481 acttgataaa ggttgacttg ccgataccat ttggaccaac gatagcgaca gcattcatct
     9541 tacgaagatc taggttgata ggttgtgaca aaacttcccc gtcataacca atagcagtat
     9601 tttcaacagt caaaacaaca ttgcccgacg ttttttcaga ctggaaggtc atgttggctg
     9661 ctttcttgcc agcttcaggc ttgtccaaac gctccatttt ttctagttgt ttacggcgag
     9721 attgagcacg tttggtcgtt gaagcacgaa ctagattgcg attgacaaag tcttccagag
     9781 cagcgatttc cttctgttgc ttttcatagt tttttgcctc agtagctagc ttttgctcct
     9841 tcaactcaac aaaacgagag taattcccca catagcgatc caaggaatac ttggtcaaat
     9901 ctagcgtaat tgtcgcaacc ttgtccaaga aataacggtc gtggctgacg ataatgaggg
     9961 caccgctata gtttaccaag taattctcta gccaggagat ggtttcaata tccaagtggt
    10021 tagttggctc gtccaagacc aagagattgg gcttttcaag gagcatttta gctagagcca
    10081 aacgagtatt ttgaccacca gaaagctcag caattttcat ctgccacata gactcgtcaa
    10141 acttgaatcc attcaaaatc gctcgaatat cagcttcata ggtaaagcca cctgcttggc
    10201 gaaaattttc agataagcgg tcataatctg acatcagttt atccaaatcc tcaccagact
    10261 tttcacccat ctccagctcc atctgacgca gttgtttatc tgtccgacgt aagtcatcaa
    10321 agacatgaag catctcatca tagatggtat tttcagactc aaaacggcta tcttgagcta
    10381 ggtaagacag agaaatatct tttttcttat tgatttctcc actagttggc tcctcttctc
    10441 caactaaaat cttcaaaaga gtagacttac ctgcaccatt tttcccaaca agggcaatcc
    10501 gatctcgttc atcaacctgc aggttgatat tatcgaaaag aacctctcct gcaaaagaac
    10561 gttcaatttt attagcttgt aaaataatca tacaagtagt atagcatgtt tccctaaggc
    10621 attcaagata atcgtaagtc ttttagtaca acttttataa cataaaataa actaaattat
    10681 gtatatttta tattagattc cttcactatc ttgttggatt ttctaaccag ctaatcttgt
    10741 ttcaaatagt tatcgcacaa gcctattatt taattctttt cattatttac gtacgc
//
LOCUS       DBEJAQ010000060        10586 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.060, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000060 DBEJAQ010000000
VERSION     DBEJAQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.060"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            122..277
                     /locus_tag="ACL297_001827"
                     /pseudo
     CDS             122..277
                     /locus_tag="ACL297_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="ACL297_001828"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="ACL297_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HGR0072036.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="ACL297_001829"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="ACL297_001829"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGR0072037.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKANFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7614..8792
                     /locus_tag="ACL297_001830"
     CDS             7614..8792
                     /locus_tag="ACL297_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072038.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            9001..>10586
                     /gene="iga"
                     /locus_tag="ACL297_001831"
     CDS             9001..>10586
                     /gene="iga"
                     /locus_tag="ACL297_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HGR0072039.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPE"
BASE COUNT         3376 a         2139 c         2241 g         2830 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct accagtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cggacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 atcatgagat gaccaaatcc cagatgagtt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaaggtc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc accttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgttga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcctgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagtat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga tttgtatact gacctcagtc
     1681 agtctgatat tgatgctttt gagcaatata tccgctatct tggtatcaat ggcttgccag
     1741 cctttcagca aatcttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttctg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggcaaaacc taaaccaagc gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggaa gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaaattgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg cagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggtcgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc ggcagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtacttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgtgctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag atagcgattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagagaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatccccaa gaaggcaagc tcattctcct caaggcaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac aagcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg aattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgcct tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaagtca agcaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     7501 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag ctgattgcgg
     7561 ctagctttct agtttgctct ttgattttta ttgagtatta gaaaggagac ctcatgacac
     7621 ttccagttag aaaatccctg cacgatgcag ttttacaggc ttcgaaagcg gatacttggg
     7681 aacaagctac caaggaatgg aatgaagttt ccttgatttt taacggtatc ggccgtagca
     7741 attgtgtctg tggaaatgcc atcaaatacg cttatgaact ctttaacggt gtcacaggcc
     7801 aacgcctctt tccaattggt agcgactgtg ttcgtcattt tcatcgattg tcccttgacc
     7861 agcaattgga agaggaagaa aaactgctca gaaaggttga aaatctaacc agaaaagctc
     7921 agaaaaagga aaaaatcaag gtcaataaaa gtgactttga cgagcgactt ctaaaatggc
     7981 tctgggaaaa aggtgttttc aaacccaatc gtggcaatca atttgcgcct gagagagact
     8041 accagctttt cctagaagtc tttcagggag gaagttggac caaggcggaa ccaaagaaaa
     8101 aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt gggaaatcgg
     8161 atgaccaact ctaccttgtc aagctaggca aggagaaaat tgactacgaa caagaactcc
     8221 gtatccaggc agagaaagaa cgcaagaaga gggataaaat cgccaagcaa tatgcagaca
     8281 atctggttct agccatggga cccgcagagc gtgcttatca agattacttt ggctttacag
     8341 agaccctgac tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa aatagagcag
     8401 aacgggctat caaggccaaa caataccaaa aagagctgga aaaagaccaa cgaattgcca
     8461 gtcaggatcc aattgaaaga aaacagaagc agacttggct cctcaattcc tattttcgtg
     8521 agcttcctga agaaaaagct cgattttcta ggctcttatt agaatatcga aaaagtggtg
     8581 aagtaccctt ttcaactgaa tacctgtccg atcatctcat cgactttttc tataaaatga
     8641 aagcctttga atttgaaatc gcaccagaac aagtccgaga ttttctaaaa aaaagcctgc
     8701 aggaagacca tcgctcatca gcacaaggaa gctggataga aggaattctc ctcaactgcc
     8761 tcaaaccatt tttagaacga ttagtgatat gagtaacacc taccgtgtaa atttcatggt
     8821 aggtgttatt gtacccccaa ataatataaa acaaagtatg aaagcgcatc tcaattttaa
     8881 gacttttttg taaattaaga gtataattaa ggtatactgc cttttctata gataatagat
     8941 aatacactaa tataataaag gtctttgtaa catatataaa ttttatttca aggaggaata
     9001 atggaaaagt attttggtga aaaacaagag cgtttttcat ttagaaaatt atcagtagga
     9061 cttgtatctg caacgatttc aagtttattt tttatgtctg tattagctag ttcatctgtg
     9121 gatgctcaag aaactgcggg agttcactat aaatatgtgg cagattcaga gctatcatca
     9181 gaagaaaaga agcagcttgt ctatgatatt ccgacatacg tggagaatga tgatgaaact
     9241 tattatcttg tttataagtt aaattctcaa aatcaactgg cggaattacc aaatactgga
     9301 agcaagaatg agaggcaagc cctagttgct ggtgctagct tagctgctct gggaatttta
     9361 atttttgctg tttccaagaa aaaggttaag aataaaacgg tattacattt agtattggtt
     9421 gcgggaatgg gaaatggtgt cttagtttca gtccatgctt tagaaaatca tcttttgcta
     9481 aattacaata cggactatga attgacctct ggagaaaaat tacctcttcc taaagagatt
     9541 tcaggttaca cttatattgg atatatcaaa gagggaaaaa cgacttctga ttttgaagta
     9601 agtaatcaag aaaaatcagc agccactcct acaaaacaac aaaaggtgga ttataatgtt
     9661 acaccaaatt ttgtagacca tccatcaaca gtacaagcta ttcaggaaca aacacctgtt
     9721 tcttcaacta agccgacaga agttcaagta gttgaaaaac ctttctctac tgaattaatc
     9781 aatccaagaa aagaagagaa acaatcttca gattctcaag aacaattagc cgaacataag
     9841 aatctagaaa cgaagaaaga ggagaagatt tctccaaagg aaaagactgg ggtaaataca
     9901 ttaaatccac aggatgaagt gctatcaggt cagttgaaca aaccggaact cttatatcgt
     9961 gacgaaacta tagaaacaaa aatagatttt caagaagaaa ttcaagaaaa tcctgattta
    10021 gcagaaggaa ctgtaagagt aaaacaagaa ggtgcattag gtaagaaagt tgaaatcgtc
    10081 agaatatttt ctgtaaacaa tgaagaagtt tcacgagaaa ttatttcaac ttcaacgact
    10141 gcgcctgttt caagaatagt cgaaaaaggt actaaaaaag ctcaagttat aaaggaacaa
    10201 gctgagactg gtgcagaaca taaggaagta cagtctggag ctattgttga acccgcaatt
    10261 cagcctgaac taccagcagc tgtattaacc gacaaaggtg agtcagcagt ccaaccagag
    10321 ttgccagaag ctgtagtaag tgataaaggc gtaccagaag ttcaaccagc gttaccagag
    10381 gcagtcatga ctgacaaggg tgatccagag caggtagaac cgctaccaga atatacagga
    10441 gtacaagctg gagctattgt tgaacctgaa aaagttgaac cggaatatgc tggagtacaa
    10501 gctggcgcta ttgttgaacc agagcaggta gcaccactac cagaatacac aggggtgcaa
    10561 gccggagcga tagtcgagcc agagaa
//
LOCUS       DBEJAQ010000061         9710 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.061, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000061 DBEJAQ010000000
VERSION     DBEJAQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.061"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..496
                     /locus_tag="ACL297_001832"
     CDS             <1..496
                     /locus_tag="ACL297_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HGR0072040.1"
                     /translation="KETSKVRPYFIGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPR
                     PFPETGMLDFQDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKV
                     HFAEFPNLIIWSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFE
                     IEVL"
     gene            653..1078
                     /gene="lacA"
                     /locus_tag="ACL297_001833"
     CDS             653..1078
                     /gene="lacA"
                     /locus_tag="ACL297_001833"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HGR0072041.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1109..1624
                     /gene="lacB"
                     /locus_tag="ACL297_001834"
     CDS             1109..1624
                     /gene="lacB"
                     /locus_tag="ACL297_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HGR0072042.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1635..2564
                     /locus_tag="ACL297_001835"
     CDS             1635..2564
                     /locus_tag="ACL297_001835"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HGR0072043.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2566..3546
                     /gene="lacD"
                     /locus_tag="ACL297_001836"
     CDS             2566..3546
                     /gene="lacD"
                     /locus_tag="ACL297_001836"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HGR0072044.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3780..4616
                     /locus_tag="ACL297_001837"
     CDS             3780..4616
                     /locus_tag="ACL297_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGR0072045.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4652..4969
                     /locus_tag="ACL297_001838"
     CDS             4652..4969
                     /locus_tag="ACL297_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGR0072046.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            4971..6665
                     /locus_tag="ACL297_001839"
     CDS             4971..6665
                     /locus_tag="ACL297_001839"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HGR0072047.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            6880..8286
                     /gene="lacG"
                     /locus_tag="ACL297_001840"
     CDS             6880..8286
                     /gene="lacG"
                     /locus_tag="ACL297_001840"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGR0072048.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8409..8588
                     /locus_tag="ACL297_001841"
     CDS             8409..8588
                     /locus_tag="ACL297_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HGR0072049.1"
                     /translation="MTSEELKSLGKWYVSTGKEWICHSDDELEEFKNLFLNFINPEEW
                     DTISFDSDFMPFQQS"
     gene            complement(8867..9628)
                     /locus_tag="ACL297_001842"
     CDS             complement(8867..9628)
                     /locus_tag="ACL297_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGR0072050.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT         3117 a         1651 c         1953 g         2989 t
ORIGIN      
        1 taaagaaact agcaaggtca ggccttactt tattggtgga catccaggat ttaattgtcc
       61 tctacttgat gatgaagtct atgaagatta ttatctagag tttgagaaag aagagacttg
      121 ctctgttcca cgtcctttcc cagaaacagg tatgttggat ttccaagata gaagtccatg
      181 gctagaggga caaaaagaaa tagatcttag ttatgatctt ttcagcaaag atgcagtgac
      241 tttggatgaa ttgcaatctc gaacaattgc ccttcgttct cttaaacatg ataggggatt
      301 gaaagtgcat tttgcagaat ttccaaacct catcatctgg tcaactttga acaagggacc
      361 tttcattacc tttgaaccat ggtctggctt gtcaacattc cttgaagaag gagatcattt
      421 agaagataag aagaatgttt gtctcttaga agccaatcag gttgaagaat tagggtttga
      481 gatagaagtt ttataaaaaa gtaaaaaata aataacaaaa acaaacacaa actgttgact
      541 tcttcaaaca atagtagtat attatgtttg tgaggactaa ttgactgttt tttaaagaca
      601 gccaatacac tgctggaatc cagcatagaa aaaataaaaa ggagtataca atatgtctat
      661 tgttatcggt gcagatgctg caggtttgag attgaaagaa gttgtgaaag acttcttaga
      721 aaaagaaaac ttccaccttg tggatgttac agctgaaggt caagactttg ttgatgtgac
      781 tctcgctgtt gctgcagaag taaacaaaga agaacaaaac cttggtatcg tgattgatgc
      841 ttatggagct ggtccattta tggttgcaac taagatcaaa ggaatggttg ctgcagaagt
      901 ttctgatgaa cgttcagctt atatgactcg tggccacaac aactcacgta tgatcactat
      961 gggtgcacaa cttgttggtg atgaattggc taaaaacatc gctaaaggat ttgttaatgg
     1021 taaatacgac ggtggtcgtc accaaatccg ggttgacatg ttgaacaaaa tgggctaatt
     1081 agattatagt aagaaaggta agttaaaaat gagaattgca attggatgtg accacatcgt
     1141 aacagatgaa aaaatggcgg tttcagaatt tttgaaatca aaaggatatg aagtcattga
     1201 ctttggtaca tatgaccata cacgtactca ctacccaatc tttggtaaaa aagtagggga
     1261 agctgtaact agcggtcaag ctgatcttgg agtatgtatc tgtggtactg gtgttggtat
     1321 caacaacgct gtaaataaag ttccaggtgt tcgttctgcc ttggttcgtg atatgacaac
     1381 agccctttat gctaaagaac aattgaacgc taacgttatt ggttttggtg gtaaaattac
     1441 tggtgaattg cttatgtgtg atatcatcga agctttcatc catgctgaat acaaaccaac
     1501 tgaagaaaac aaaaaattga ttgcgaaaat tgaacatgtt gaaagtcaca atgctcaaca
     1561 aacagacgca aacttcttca ctgaattcct tgaaaaatgg gatcgcggag aataccacga
     1621 ctaagaggta acctatgatt ttaacagtta caatgaaccc atccattgat atttcctatc
     1681 ccttggatga gttgaagatt gatactgtca atcgtgtggt ggatgtaacc aaaacggctg
     1741 gtggtaaggg actcaatgtt acccgagtac tttcagaatt tggcgattct gttcttgcta
     1801 ctggtttagt gggtggcaaa cttggtgagt ttttggttga acatatcgat aatcaagtaa
     1861 agaaagattt cttctcaatt cagggagaaa ctcgtaactg tatcgctatt ctccacggag
     1921 acaaccaaac agaagttctt gaaaaaggtc ctgaagtatt ggaacaggaa ggtcaagact
     1981 ttttggaaca tttcaaaaaa ctcttggagt cagttgaagt agtggccatc tcaggtagtc
     2041 tgccagctgg ccttccagtt gattactatg cgagcttggt agaacttgct aatcaagctg
     2101 gcaagcctgt agtcttggac tgctcaggtg cagcacttca ggctgttctt gaatcacccc
     2161 ataaaccaac agtcatcaaa ccaaataatg aagaattgtc tcagcttctt ggaagagaag
     2221 tttctgagga tttggatgaa ttaaaagaag tacttcaaga acctttgttt gcagggattg
     2281 aatggattat cgtttcactt ggtgccaacg gtacttttgc caaacatggt gacactttct
     2341 acaaggtaga tattcctaga attcaggtgg taaatcctgt tggatctgga gactctactg
     2401 tggcaggaat ttcttcagga cttcttcaca aagaatcgga tgcagaatta ctcatcaagg
     2461 caaatgtcct tggtatgctc aatgctcaag aaaaaatgac tggtcatgtc aacatggcca
     2521 actatcaagt tctatatgat caattaatag taaaagaggt ataaaatggc tttaacagaa
     2581 caaaaacgtg tacgcttaga aaaactttct gatgaaaatg gtatcatctc agctcttgca
     2641 tttgaccaac gtggtgcttt gaaacgcctc atggctcaac accaaacaga agaaccaact
     2701 gtggcccaaa tggaagaact taaagtcttg gtagcagatg aattgactaa atacgcttca
     2761 tctatgcttc ttgaccctga gtatggactt ccagcaacta aagctcttga tgaaaaagct
     2821 ggtcttctcc ttgcttatga aaaaacaggt tatgacacaa caagcacaaa acgcttgcca
     2881 gactgcttgg atgtctggtc tgcaaaacgt attaaagaag aaggtgcaga tgcagttaaa
     2941 ttcttgcttt actatgatgt agatagctca gacgaactca atcaagaaaa acaagcctac
     3001 atcgaacgca tcggttctga gtgtgtggct gaagatatcc cattcttcct tgaaattctt
     3061 gcttacgatg aaaaaattgc ggatgcaggt tctgtagaat acgcgaaagt aaaaccacac
     3121 aaagttatcg gcgctatgaa agtcttttca gacccacgct ttaacattga tgtcttgaaa
     3181 gttgaagttc ctgttaacat taaatatgtt gaaggcttcg ctgaaggtga agtagtttat
     3241 acacgtgaag aagcagcagc cttcttcaaa gcgcaagatg aagcaacgaa cttgccatac
     3301 atctacttga gtgctggtgt atcagctaaa ctcttccaag atactcttgt atttgctcat
     3361 gaatcaggtg caaactttaa cggagttctt tgtggccgtg caacatgggc tggatcagtt
     3421 gaagcataca tcaaagatgg tgaaacagca gctcgcgaat ggcttcgcac aactggattt
     3481 gaaaacattg atgagctcaa caaagttctt caaacaacag caacttcatg gaaagaacgt
     3541 gtgtaagaaa gtcctcctag tttaggaaca tgaatctaaa aaaatttaaa aaaagttgta
     3601 tgtaaaggtt tacaaaataa cttacttgtg ctatacttaa atcacaagtt aatacaaggt
     3661 gagtgttact aagtaatatt aggcatgatc acaggtgaat tagaaatcag ctgattttct
     3721 agttcatttg tggtcatttt ttgtactcat atacctttaa gatataaaag gaggttgaca
     3781 tgtatcgaat tctaaatcca atgaatcaca atgtctcgct tgtcagaaat gataagggag
     3841 aagaggtgat tgtaattggt aaggggattg cattcggaaa gaagaagggg gatttgattg
     3901 ctgaaaatca tgttgagaaa atctttcgga tgaagaccga agagtccaga gaaaacttta
     3961 tggctcttct caaagatgtt ccgcttgatt ttatcacagt gacctatgaa atcattgata
     4021 agctatcaaa gaaatatcat tatccgattc aagagtatct ctatgtaacc ttgacagatc
     4081 atatttactg ttcttatcaa gctctaactc aaggaaggta caaggatagt aatctgccag
     4141 atatttctgc taagtatcct gtcgcttttc aaatcgcaaa tgaagctttt gaaatttact
     4201 gtcagaagct agcagatcat tttcctgagg acgaaattat tcggattgct tatcatttca
     4261 ttaatgctga aggtgaaaat gaagtggaac ttgtggagtc gattgataag aggaaagaaa
     4321 ttctcaggaa tgttgaagaa gttttaacgg actatgcaat tcaacgaact aaaaagaata
     4381 accatttcta tgatcgcttt atgatccatt tgaattattt cttggattat ttagacagat
     4441 ctagagatga taaccaatca cttctggata tggaagatca tattaaacaa tcctatccaa
     4501 aagccttcga gattggttcc aagatctatg atgtgattac gcaacatacg ggtcttgatt
     4561 tgtataaaag tgaacgagtt tatctagttc tacatatcca acgtttattg tcataaaaat
     4621 ttatttaaaa ctatataagg agaattctat catgaataga gaagaagtaa cattgttagg
     4681 ttttgaaatc gtagcctatg ctggcgatgc tcgttcaaaa ctattggaag ccttgaaggc
     4741 tgctgaagct ggtgattttg aaaaagcgga cgctctggta gaggaagctg gtagctgtat
     4801 tgcagaggct caccacgcgc aaacaagtct attgactaag gaagcttctg gtgaggactt
     4861 agcgtatagt gtgaccatga tgcatggtca agatcacttg atgacaacta tcttgttaaa
     4921 agacctaatg caccatttaa ttgaacttta caagagagga gttaaataaa atggataagt
     4981 taataatttt tattgagaaa ggaaagcctt tctttgagaa attatcgaga aatatatact
     5041 taagagcgat aaaagatggt tttatttcca gtatgccagc agtattattc tcaagtattt
     5101 ttatactaat agctgctgtg cccaatattt ttgggtttaa atggtctgat gaacagttag
     5161 cttttatttt gaaaccttac aactactcaa tggggatttt agctctctta gtagctggga
     5221 caactgcaaa atcgttaaca gattcagtta atacacgtag tatggagaaa accaaccaaa
     5281 tcaactacat gtcaactttt ttagcagctg ttgttggatt gttaatactg gcagctgatc
     5341 caattgaagg tggatttgct aatggtcttt taggtactag aggattactt actgcatttt
     5401 tggcagcttt tattacagta aatatctata aagtatgtat taaaaataat gtaaccatta
     5461 gacttcctga agaggttcca ccaaatattg cacaagtttt taaagatgtt attccgtttg
     5521 ccttatctgt tctttcaata tatggattgg atttaattgt tagaaatatt tttggtacaa
     5581 atgtagcaga atctgttggt aaaatattag cccctctatt ttcagctact gatggttata
     5641 tcggtttagc tattgtattt ggtgcttatg ctttcttttg gtttgttgga attcatgggc
     5701 catctgttgt agaaccttta attgtagcta tcagttatgc aaatatagaa gctaatgttc
     5761 agcttgtgca agctggtatg cacgcagata aaattttaaa tccagttact caaacttttg
     5821 ttgtaacaat gggtggtact ggtgcaacac tagtagttcc atttatgttt atgtggttat
     5881 gtaaatcaaa aagaaataga attgtcggac gtgcgtctgt agttcctact ttctttggcg
     5941 taaatgaacc aatcctattt ggtgctccaa ttgttttaaa tcctatcttc tttattccgt
     6001 ttgttactgc tcctattatc aatgtttgga ttatgaaatt ttttgtagat gttctacaaa
     6061 tgaatagttt cagtattatt ttaccttgga caacacctgc tccaattggt attgttatgg
     6121 gaacggcgct ggctccattg tcattcgtct tggctataac tttaatcatt atcgacactt
     6181 taatctatta tccatttgtt aaagtttatg accatcaaat tttagaagaa gaacggaaag
     6241 gaaactcttc atctgaattg aaagaaaaag ttgctgcaaa cttcaacact gcaaaagcgg
     6301 atgctattct tgaaaaagca ggtgtcgatg cagcacaaaa taccatcact gaagaaacaa
     6361 atgtcctcgt tctctgtgct ggtggaggta caagtggact ccttgcaaat gctttgaata
     6421 aggcagcagc agaatacaat gtccctgtga aagcagcagc aggcggctat ggtgctcacc
     6481 gtgaaatgtt accagagttt gatttggtta tccttgctcc tcaagttgct tcaaactttg
     6541 aagatatgaa ggcagaaaca gataagctcg gtattaaact agcgaaaaca gaaggcgctc
     6601 aatacatcaa attaactcgt gatggaaaag gtgccctagc attcgtacaa gaacaattcg
     6661 attaaggcta ggaactgtga aacagtctcc tatcgttacg gaaatcgcta tggcgaattt
     6721 cctaatcgcc ttattaattc gtcggtaaaa agatatcgtt tttacctcct catgtcacaa
     6781 ttcggtgact tggtacaaga agtgagatgg agaaggatgg ctcactgact cctctcctct
     6841 cacttttact ttatttaaat caagaaatag gtgaaaaaaa tgacaaaaac acttccaaaa
     6901 gactttattt ttggtggcgc aacagctgcc tatcaagcag aaggtgctac acatactgat
     6961 ggtaaaggac cagtagcgtg ggataaatat cttgaagata actactggta cactgcagaa
     7021 ccagccagtg atttttacaa tcgatatcca gttgatctca agctagcaga agagtatggt
     7081 gtcaatggca ttcgaatttc tattgcctgg tcacgtattt tcccgactgg ttatggtcaa
     7141 gtaaatgcta aaggtgttga gttttatcat aatttatttg cagagtgtca caaacgtcat
     7201 gttgagccct ttgtaactct tcatcacttt gacacgccag aagctctcca ctcaaatgga
     7261 gacttcttaa accgtgaaaa tatcgaacat tttgtagact acgctgcctt ctgttttgaa
     7321 gaatttccag aagtaaacta ttggacaacc ttcaatgaaa ttggaccaat cggtgatggt
     7381 caatatttgg ttgggaaatt ccctccaggt atccagtacg accttgccaa agtctttcaa
     7441 tcacaccaca atatgatggt gtctcatgca tgcgcggtaa aattgtacaa agagaaaggc
     7501 tataaagggg aaattggtgt tgttcacgcc ctgcctacta aatatcctct agatcctgaa
     7561 aatccagcag atgttcgtgc agcagagttg gaagatatca tccacaataa attcatctta
     7621 gacgcaactt atctaggtcg ctattcagct gaaaccatgg aaggtgtcaa ccatatctta
     7681 tcagtcaatg gtggtagttt ggatcttcgt gaagaagatt ttacagcatt agaagctgca
     7741 aaagacttga atgatttcct aggaatcaac tactatatga gtgactggat ggaagccttt
     7801 gatggagaaa ctgaaattat ccataatggt aaaggtgaaa aaggaagctc taagtatcaa
     7861 atcaaaggta ttggtcgtcg tgtagctcct gactatgtat cacgcacgga ttgggattgg
     7921 attatctacc ctcaaggttt gtatgaccaa atcatgcgtg tgaagaaaga ttatcctaac
     7981 tacaagaaga tttacatcac tgaaaatggt ctcggctata aagatgagtt cgttgataac
     8041 actgtttacg atgatggtcg tattgattac gtgaaacaac acttggagat cttgtctgat
     8101 gcgattgcag atggagctaa tgtaaaaggt tacttcattt ggtcattaat ggatgtcttc
     8161 tcatggtcaa acggttatga gaaacgttat ggtctcttct acgtagattt tgaaactcaa
     8221 gaacgttatc ctaagaaatc agctcactgg tacaagaaag tagcggaaac tcagattata
     8281 gactagtaga attagtcatt agatatagaa ttttagtgag tcaaaaagat gttcaaagat
     8341 tttatccaat ctatttatga aaaaaagttt atattataaa tttcgaaaaa tgctctcaaa
     8401 taccgtgttt gacgagtgaa gaattgaaaa gtcttggaaa atggtatgtc tcgactggta
     8461 aagaatggat ttgtcattca gatgatgagc tggaagaatt taaaaatcta tttttaaatt
     8521 ttatcaatcc tgaagaatgg gatactatct cctttgattc agattttatg ccgtttcaac
     8581 aatcgtaacc aatttctcaa aaaagttaaa tcttatattt agtactctgt aaaactctta
     8641 tctaatcacg ttgcttatac tcaatgaaaa tcaaagagca actttaaact aggaagcgag
     8701 tcgcagattt ctcaatgcat agctttgagg aattgggcaa aaagtctttg atatagaaaa
     8761 aagcatagta tcaggtgttg aaacacctga tactatgctt tttattgtgg gaagatttac
     8821 tttttttctt ctgaaattga gttgttaccc aggctctttc agtttattaa ggcttgatga
     8881 ctttaatgtg tttagatagc ttaaaaagga ttgaatcact tagtttagaa tctgaaacaa
     8941 tagtatcaag atttgatata ttataaaaag tataaaaatc aaacttattg aacttactat
     9001 gatctgcgag taaatatttt ttattagaat tatttaaagc gatgcgttga gcctctccct
     9061 cttcctcgct aaaagtagct agagctccgt tttgaatacc attacagcta acgaaagctt
     9121 tagaaaattg gagattagag agattttgta gggtcaatgt gccaacaaaa gcacctgtaa
     9181 tatcgcgata atttccacct atcaaaatca aatctgttaa ttttcgttcg cttaaaatca
     9241 gaaaaacagg tagactgttg gttacgacgc ggatattgtc aataggcaac tcacgcgcaa
     9301 aaaactctaa tgttgttcct ggtccaatga aaatagtttc tctttcttct actagactgc
     9361 ctgcaaaatg ggctatttct tgtttttctg ccgtttggag ggcttgtttt tcaatatttg
     9421 atcgctcatt agtcaaaagg gagttggttc gaagtttttc agctccacca tgcacacgaa
     9481 tcagcaaatc tttatcagct aattcctgta aatagcgcct tgcagtcata tctgaaacgg
     9541 ctatttcgtc cataatctgt ttaactgtta tggttccttt actatttact atctctaaaa
     9601 ttttggttaa tttttcttgt ttgagcatat tatcacctcg tttcctacta ctatcttacc
     9661 ataaacaaac tcatcattca aatacaaaaa caacaaaatg aaacaaaaac
//
LOCUS       DBEJAQ010000062         9530 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.062, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000062 DBEJAQ010000000
VERSION     DBEJAQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.062"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..463
                     /locus_tag="ACL297_001843"
     CDS             <1..463
                     /locus_tag="ACL297_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="HGR0072051.1"
                     /translation="RLPTFAFIFAKLSEKKLEYMMLGDCVMLVNEMEITDHRVDNLFE
                     KGKNEIKDPIGTNSVLNKKIILQKIRKLSNQPSGYWIGSLDERFLDHAIINQIDVTSE
                     QIVLMSDGFYEFYQNNQNKTFEELIKMRFNSSAIDPIYGKKDDASIVVIDV"
     gene            521..1162
                     /locus_tag="ACL297_001844"
     CDS             521..1162
                     /locus_tag="ACL297_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HGR0072052.1"
                     /translation="MLRLYLENEIIELSNNLETIWVSVLDKYEKLFEYLSNEERIELI
                     KEDLIINESVLNVDKKGYELICLQHPVSYDLRRIISVIKISTDIERIGDRIVEILKNL
                     QIIQNNEILKKIISEIKILHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELST
                     NIEKKIMNYIFEDDGNVSEVIGALDIIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            1175..2650
                     /gene="galT"
                     /locus_tag="ACL297_001845"
     CDS             1175..2650
                     /gene="galT"
                     /locus_tag="ACL297_001845"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGR0072053.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            2663..3643
                     /gene="galE"
                     /locus_tag="ACL297_001846"
     CDS             2663..3643
                     /gene="galE"
                     /locus_tag="ACL297_001846"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGR0072054.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGLPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
     gene            3853..4512
                     /locus_tag="ACL297_001847"
     CDS             3853..4512
                     /locus_tag="ACL297_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HGR0072055.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            4537..5604
                     /locus_tag="ACL297_001848"
     CDS             4537..5604
                     /locus_tag="ACL297_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGR0072056.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELASKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWGVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            5707..6717
                     /locus_tag="ACL297_001849"
     CDS             5707..6717
                     /locus_tag="ACL297_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0072057.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            6729..8420
                     /locus_tag="ACL297_001850"
     CDS             6729..8420
                     /locus_tag="ACL297_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0072058.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            8423..9133
                     /locus_tag="ACL297_001851"
     CDS             8423..9133
                     /locus_tag="ACL297_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGR0072059.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            9229..>9530
                     /locus_tag="ACL297_001852"
     CDS             9229..>9530
                     /locus_tag="ACL297_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072060.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVG"
BASE COUNT         3283 a         1265 c         1837 g         3145 t
ORIGIN      
        1 tcgcttacca acatttgctt ttatatttgc taaactttca gaaaaaaagt tggagtatat
       61 gatgctaggt gactgcgtca tgttagtaaa cgaaatggaa ataacggatc atagagtaga
      121 taacttattt gaaaaaggga aaaatgagat aaaagatcca attggtacaa atagtgtttt
      181 aaataaaaaa attattttac aaaaaatcag aaaattatct aatcaaccga gtgggtactg
      241 gataggttcg ttggatgaga gatttttaga ccatgctatt attaatcaaa tagatgttac
      301 tagtgaacaa attgttttaa tgagcgatgg cttttatgaa ttctatcaaa ataatcaaaa
      361 taaaactttt gaagagttaa ttaagatgag atttaattct tctgctattg atccaatcta
      421 tggaaagaaa gatgatgcaa gtattgtagt tattgatgtt tgatatattg tttaaaatat
      481 taattgttac tattgaactt tgttaagaag aggtacctaa atgttaaggt tatatttaga
      541 aaatgaaatt atagaattat caaataattt agagaccatt tgggtatctg tgcttgataa
      601 atatgagaag ttatttgaat atttatcaaa tgaagaacga atagaattga ttaaagaaga
      661 tttaatcata aatgagtcag ttctaaatgt tgataaaaaa ggatatgagt taatttgttt
      721 acaacatccc gtttcatatg acttaagaag aattattagt gtaataaaaa tatcaactga
      781 cattgaacgt attggggata gaattgttga aattttaaaa aatctacaga ttattcaaaa
      841 taatgaaatt ttgaagaaaa ttatttcaga aataaaaata cttcatgaag ttatcggact
      901 gcatatgaat agggctatat cttgttatag agaggaacaa tctggatgtt tagatatggt
      961 tgttatacaa aaacagaatg agatagaaga gcttagtaca aatattgaaa agaaaataat
     1021 gaattacata tttgaagatg atgggaatgt ttctgaagta attggtgctt tagatattat
     1081 tcatcacctt gataaaattg ctcatactac gcaatcaatt tataaatgga taatgtatag
     1141 aaaatatggg aacataaatt agaaagaaaa atatatgaaa acaattattg ataattttgt
     1201 tgatagagta attgaatacg gaatgtataa tgaaattgac aaaatatatg tgaaaaatag
     1261 agtactagcg ttaataggtg aggagggaac tgatcgcatt tccgatgaaa atgacctgaa
     1321 acaaataaaa gattacttag tggaaatcgc cttaaaaaac ggtaaaatta aagatttaat
     1381 cgaggaaaag gaatgtttag gagcagagtt aatgaatttc atagttcctt tacctagtcg
     1441 attaaatgat attttttgga gttcatatga tatatcacct caagaagcag ttgaagagtt
     1501 ttataagtta agtaaagata gtgattatat taaaacttct gctattgcta agaatattga
     1561 gtttcgagca tcaactaaat acggagagct agagattacg ataaatttgt caaaacccga
     1621 aaaagatcca aaaacaattg ctgctgaaaa attagtaaaa gccactaatt atcccaaatg
     1681 tttactatgt atggaaaatg aaggatatca ggggcgaata aattatccag cacgttcgaa
     1741 tcatagaatt attcgattga aacttggtga cgaagtatgg ggattccaat attctcccta
     1801 cttatacttt aatgagcatg cgatattttt aaatagtcaa catgtaccaa tggctattac
     1861 ctctaaaaca tttgaacaat tattggagat tgttgatatt ttgccaggat attttgctgg
     1921 ttcaaattct gatcttccta tctcaggagg ctccattctt agtcataacc actaccaggg
     1981 agggaaacat attttcccaa tggaaaaagc aaaatttgag agtgaatttc gttttaaaga
     2041 ctttgaggat gttaatgctg gtattgtaaa atggccaatg tcagtaatta ggttacaaag
     2101 tgaaaataaa aatcgcttgt tagatttagc tactaaaatt ctaaataagt ggagagaata
     2161 ttcagattta gaagtagaca tcattgctat gactgaagat gtcccacacc atacggtgac
     2221 tcctattgct cgtaaagtgg acgggagata tgaattagat attgttctaa gagataatca
     2281 tactactgaa cagtacccag atggtgtttt ccatcctcat caagatgtac aacatattaa
     2341 aaaggaaaat atcgggttaa tcgaagtgat gggacttgcg atattaccac ctcgtttgaa
     2401 accagagtta gaagaagttg gaaaatatct tttaggagag gataacgcaa ttgcggatta
     2461 tcatttagaa tgggccgacc aattgaaaga aaaatatcct cgcattaata aagaagaggt
     2521 taatagcgta gttcaacacg aagcaggaca agtgtttgcg agagttctgg aagatgcagg
     2581 agtttataag aatacaccgt ctgggcatga agcgtttatg agatttgtta agtcggttgg
     2641 aattaattag gaggaaacaa aaatggcaat attggtaaca ggcggagctg gttatattgg
     2701 tagccatacc gtagtagaat tactaaattt aggaaaggaa gtcatcattg tcgacaacct
     2761 ttcgaactct agcatcttgg tgctagaccg tattgaagca attacaggaa tacgtcccgt
     2821 gttttacgaa ttagatgttt gtgataaacc agcattgaga aaggtatttg aacaagaatc
     2881 gattgatgct gcaattcatt ttgcaggtta taaagctgtc ggagaatccg tgcaaaagcc
     2941 tgtgatgtac tacaaaaata atattatgag tacattggca cttgttgaag tgatgtcaga
     3001 atttaatgtt aaaaagattg tcttttcttc aagtgcgact gtatatggaa ttaacaatca
     3061 gtcacctcta attgagacga tgcaaacaag tgcgacaaat ccttatgggt atacgaaagt
     3121 gatgcttgag caaattttaa aagatgttca tgtggcagat tcagaatgga gtattgcgtt
     3181 gcttcgttat ttcaatccaa ttggtgctca tgagtctggt ttgattgggg aagatccctc
     3241 aggacttcct aacaacttga tgccttatat tgcacaagta gcggtaggta agctatcaga
     3301 gctaagtgtg ttcggaaatg attatgatac gcttgatggt actggtgtgc gtgattatat
     3361 ccatgtagta gatttagcaa taggacatat aaaagcttta gaaaaagtat ctgaaaaaac
     3421 agatgtttat atctataacc taggctcggg agaaggcact agtgtattac aacttgtaaa
     3481 tacatttgaa agtgttaata agatccctat tccttataaa atagttccaa gacgttcagg
     3541 agacgttgca acttgttatg caaatgcaga caaagcatat aaggaattaa attggaggac
     3601 aacaaaatcg attgaagaca tgtgtagaga tacatggaat tagcaatcaa aaaatcccaa
     3661 tggctataat tgatgatact gtctatatga gtgctgtatt ttggaaaaat tacttaacct
     3721 tttaatgaaa tgcatattta atgaaagata aattaaaaaa aatgtttgac agcgcttgac
     3781 aaaggaaacg gtttcattgt ataatgtaaa catagtgaca ccgatgtcaa atataaaaaa
     3841 gaaggtcttt atatgaaaaa cttaacaaaa attaagttca aagaaaatgg ggaatttaat
     3901 catttccctg ggaatacagt tgtagcaaat ctttatacta aacaagattt gatggaagtt
     3961 gttgatatta ttcaatcacg ttatagagaa ttaccattta tcgataagtt tactttaact
     4021 ccaaggaatt ctattcacat gactgtaatt gaattgttgt gccatgaaaa tcgtgaaacg
     4081 gagttttgga gcagtaatct tcctctagat acacctttac aggaaataca tgattacttt
     4141 gctaaacaac ttgaaatttt tccattgttg gatgaagaaa ttcatatgcg tataactgaa
     4201 atgggaaaac aaaacatact agttgaacct gcagatgaag cttctgcaaa gagattagaa
     4261 gaaattcgta cttatgtctc agaaaaagca ggtgtttgtt tccctaatca tgatagatat
     4321 caattccata tttcaattgg gtatcttcgg attcctctaa ccgaagagga ggaagaagag
     4381 tttactaaag tcagagcaga attaactgaa attttattag agaagattcc aactattact
     4441 gtaaaccgta ttgactatac tgtatttgaa gatatgagac aatttgttcc atatcacgaa
     4501 aaatttaaat aaaataataa aaaaggagaa cttatcatga aaaaaatgaa agtttggtct
     4561 actgtacttg caacgggagt tgctcttact acacttgctg cttgctctgg aggttcaaat
     4621 tctacgactg cttcttcatc tgaagaaaaa gctgataaaa gtcaagaatt agttatctat
     4681 tcgaactcag tctcaaatgg tcgtggtgat tggttaactg ctaaagcaaa agaagctggt
     4741 tttaatataa aaatggttga tatcgctggc gctcaattag cagaccgtgt tattgctgag
     4801 aagaataatg cagttgcaga tatggtattt ggaattggtg ctgttgactc aaataaaatt
     4861 agagatcaaa aattactagt acagtacaag cctaaatggt tagataaaat tgatcaatct
     4921 ttatcagata aagataatta ttataatcct gtgattgttc aaccattagt tttaattggg
     4981 gcgcctgatg taaaagaaat gcctaaagac tggacagagt tagctagtaa gtataaaggt
     5041 aaatattcaa tttatggtct tcaaggtggt acaggacggg caattctagc aagtatctta
     5101 gttcgttacc ttgatgaaaa aggagattta ggcgtatcag aaaaaggatg gggagttgca
     5161 aaagaatact ttgcaaatgc ctatactctt caaaaaggtg aaagctcagt tgttaaagcg
     5221 ttagataaag aaagcccaat tcaatacggt atgatctggg gttctggagc gttaatcgga
     5281 caaaaagaac aaaatgttga attcaaagtt atgactcctg agattggtgt accatttgta
     5341 actgaacaaa ctatggtttt aagcactagt aaaaaacaag cgttagctaa agaatttatt
     5401 gattggtttg gtcaatcaga aattcaagta gaatatagta aaaaatttgg atctattcct
     5461 gcaaataaag atgccctaaa agagttacct gaagatacga aaaaatttgt tgatcaagtg
     5521 aaaccacaaa atattgactg ggaagctgtt ggaaagcatt tggatgaatg ggtagaaaaa
     5581 gctgaattag aatacgtaaa ataagaaata agagttattt tttatgtcca agtctctcta
     5641 ttatttctaa atttaaggaa gaaataaaga gaggcttttg gtttagtaat agagggagtt
     5701 agtgagatga ttaaatttga taatattcaa attaaatatg gtgattttgt tgcaattgat
     5761 aatctgaatt tagatataca tgaaggggaa ttctttacat ttcttgggcc ttcaggatgt
     5821 ggtaaatcaa ctactttgag agcattggta ggttttttag atccatcatc aggaagtatt
     5881 gaagttaatg gaacagatgt cactcatttg gaacctgaaa agcgtggaat tggtattgta
     5941 tttcaatctt atgcgctatt tccaactatg actgtttttg ataatattgc atttggttta
     6001 aaagttaaga aggtagctcc agatgttatt aaagctaaag tatcagcagt ggcagcaaaa
     6061 attaagatct ctgatcaaca gttacagcgt aatgtatcag aattatctgg gggtcaacaa
     6121 caaagggtag cattggctcg tgctctggtt cttgaaccta aaattctttg tctagatgaa
     6181 ccattgtcaa accttgacgc aaaattacgt gtagatttga gaaaagagtt gaaaagactt
     6241 caaaaagagt taggtattac tactttatat gttactcatg atcaagagga agccttgact
     6301 ttatctgata gaattgcagt ctttaacaat ggatacatcg aacaggtcgg tacaccagta
     6361 gagatttatc ataattctca aactgaattt gtatgtgatt ttattggaga tattaatgtt
     6421 ttgaccgatg aaacagtcca cgaagtatta ctgaaaaata caagcgtttt cttagaggat
     6481 aaaaaaggtt acattcgatt agagaaagtt cgattcaatc gtgaaactga acaagatttt
     6541 attctaaaag ggacaattat tgatgttgag ttttctggag ttacaattca ctatacaata
     6601 aaagtttctg aaagtcagat tcttaatgta acaagtattg atagtcaggc tgctattaga
     6661 tctgtcggag aaagtgtgga attatttatc acaccatcag acgttctgca attttaagga
     6721 ggtgcagtat gcgtcataaa ttaaatttaa aagattggct tattcgttta gggttaatct
     6781 ggttcttagt aacatttatt atttatccaa actttgatct agtagtgaat gtatttgtaa
     6841 aaggaggaga attttccctt gatgctgtac atcgtgttct aaaatctcag agggcgcttc
     6901 agagtattat gaacagtttt aagttagcat tttcactcat tattacagtt aatgtcgtag
     6961 gtattctttg tgttctattt acagagtact ttgatattaa aggtgctaaa attttaaaat
     7021 taggttatat gacctcttta atttatggag gagtggtttt agcgactgga tataaatttg
     7081 tctatggtcc ttatggattg attacaaaat ttttacaaaa tgttatccct tctttagact
     7141 ctaactggtt tattgggtat ggtgcagtct tattcattat gacattttca ggaactgcta
     7201 atcatacatt gtttttaaca aatacaattc gaagcgttga ctatcacact attgaggctg
     7261 ctcgaaatat gggagcaaaa ccatttactg ttttccgaaa agtagtgtta ccaaccttaa
     7321 ttccaactct atttgcactt actattatgg tttttcttag tggtttatct gcagtagcag
     7381 cacccatgat tgttggtggt aaagaatttc aaactataaa tccaatgatt attacatttg
     7441 cagggatggg gaattctcgt gatttagctg ccttacttgc aattatttta ggtattgcaa
     7501 ctacaatttt gcttactatc atgaataaga tagaaaaagg tggaaattat atttctatct
     7561 ctaagactaa agcgcctctt aaaaaacaaa aaattgcgtc taagccttgg aatatcattg
     7621 ctcacattgt agcatatgga ttgttcacag ttttcatgct tccactaatt tttatagtat
     7681 tatactcatt tacagatcca gttgcaattc aaacaggtaa cttaacatta tcaaacttta
     7741 ctttagaaaa ttatcgctta ttctttagta atagtgcggc attctctcca ttcttggtca
     7801 gctttattta ttctattatt gctgcgacaa cagcaacaat tctcgcagtt gtatttgctc
     7861 gtgttgtcag aaaacataaa tctcgttttg atttcttatt tgaatatggt gctctacttc
     7921 cttggttact accaagtaca cttttagcag taagtttatt atttactttt aatcagccac
     7981 aatttcttgt cttgaatcag attttggtag gtagtttggt aattctactt attgcatata
     8041 tagttgtaaa aatcccattt tcttatagaa tggtacgtgc tattttattt agtgttgatg
     8101 atgagatgga agatgcagca agaagtatgg gtgcttcacc tttttatact atgatgaagg
     8161 ttatcattcc atttatttta ccggttgttc tctctgttat tgctttaaac tttaactctt
     8221 tattaactga cttcgactta tctgtattcc tttaccatcc cctagctcaa ccattaggta
     8281 ttacgattcg atctgcaggt gatgaaacag caacatctaa tgcacaagct ctggtatttg
     8341 tttatacaat tgttctgatg atcatttctg gaacggtatt atacttcaca caaagaccgg
     8401 ggcgtaaagt aaggaaataa tcatgacagc cactagtctt gggttatcaa atattgaaat
     8461 agttgtcagg attgttttat cagtagtcat tggtagtata attggtttag agagagggag
     8521 caaatcccag cctgcaggca tccgaactta tagtattgtt tgtctagctg catgtttgat
     8581 tatgatgacg aatgaatacg tatcttataa atttgggaca ggagatccta cacgattagg
     8641 agctcaagtt atatcaggtg tgggttttct aggcgctgga acgattctta ttacagataa
     8701 aaagaaaatt acaggtctga caactgcagc aggcatttgg gcttcggcag gaattggatt
     8761 agctattgga gtaggttttt atgagggagc tcttttagta gccatttctg tttggggtgt
     8821 gatatccatg ttccaaccac taaaaaaata tctgcaaaat cgttctaaaa tgattgaatt
     8881 gtatatagtg gttaaatcta cagaagccta taatcgtgta ttggtttatt gtgcagaaaa
     8941 tggtatcaga atgaccgatt caagaacagc gtttggagat gttaattctg acagaattga
     9001 atactttgat gttccggaca aaaaaatagc gtcatttatt actctggaat tatcaggtag
     9061 atttgaacat cttcgactga tggaagaaat tgctaatatt gtaggtgtta tttatgtcga
     9121 agaaattagc tgaaatttag taaacaaagg gacagatgtt tttgtccttt tgttttttag
     9181 atgttttaat catctaattt ttgagtagat ttttgctcag aggtttttat gaaagattat
     9241 aaaatcaatt ttgatttagg taaaatagaa tattttgata ataattgttt gatacaagta
     9301 tacaaattta tttcttttta tgatatttgt gaaatggtgt ttgcctttca tttacctcct
     9361 gatgagttga taacaaatgt tatctttaaa gaaaaaatta actctatgtt aaaatgttac
     9421 attgacaggc tattatatgt ttttataaat cctacacatt ttactgaaaa agttaatcta
     9481 caattttatg gtagtttttt ctcatatgaa tttatttgtc gtgaagttgg
//
LOCUS       DBEJAQ010000063         9050 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.063, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000063 DBEJAQ010000000
VERSION     DBEJAQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.063"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            1..213
                     /locus_tag="ACL297_001853"
                     /pseudo
     CDS             1..213
                     /locus_tag="ACL297_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            299..511
                     /locus_tag="ACL297_001854"
                     /pseudo
     CDS             299..511
                     /locus_tag="ACL297_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            411..857
                     /locus_tag="ACL297_001855"
     CDS             411..857
                     /locus_tag="ACL297_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGR0072061.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(995..2299)
                     /gene="eno"
                     /locus_tag="ACL297_001856"
     CDS             complement(995..2299)
                     /gene="eno"
                     /locus_tag="ACL297_001856"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HGR0072062.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2463..2909
                     /locus_tag="ACL297_001857"
     CDS             2463..2909
                     /locus_tag="ACL297_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HGR0072063.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2906..3709)
                     /locus_tag="ACL297_001858"
     CDS             complement(2906..3709)
                     /locus_tag="ACL297_001858"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGR0072064.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3718..4047)
                     /locus_tag="ACL297_001859"
     CDS             complement(3718..4047)
                     /locus_tag="ACL297_001859"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HGR0072065.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(3992..4363)
                     /locus_tag="ACL297_001860"
     CDS             complement(3992..4363)
                     /locus_tag="ACL297_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HGR0072066.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4422..5855)
                     /gene="glgA"
                     /locus_tag="ACL297_001861"
     CDS             complement(4422..5855)
                     /gene="glgA"
                     /locus_tag="ACL297_001861"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGR0072067.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5852..6991)
                     /gene="glgD"
                     /locus_tag="ACL297_001862"
     CDS             complement(5852..6991)
                     /gene="glgD"
                     /locus_tag="ACL297_001862"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGR0072068.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6981..8123)
                     /locus_tag="ACL297_001863"
     CDS             complement(6981..8123)
                     /locus_tag="ACL297_001863"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGR0072069.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8113..>9050)
                     /locus_tag="ACL297_001864"
     CDS             complement(8113..>9050)
                     /locus_tag="ACL297_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HGR0072070.1"
                     /translation="GYYFLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFD
                     YKWNMGWMNDILRFYEEDPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMM
                     HKMWGDRYNQFAGLRNLYTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMN
                     AKMKYFTSQLNQFYKDHRCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCI
                     FNMVPVERKDFTIGLPVAGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLT
                     FTLPAMGASVWKIKRRLKSTKTVTNKNQKGVENEK"
BASE COUNT         2757 a         1980 c         1607 g         2706 t
ORIGIN      
        1 tactatgaac aagacccaga aaaagtagcc ttatttctta agaattttaa tagtttaaag
       61 cacctagcac aggtctaaca aatgaatggt gagttaatcg ctccaatgac ttacgaagag
      121 acgatgacga gcgacttttt tgaagcttgg tttcagaaat ttctcttacc aacattaacc
      181 gcaccattgg ttattatatt aaaacgaagt aagaatagga caaatcgatc aggacagtaa
      241 aatctaattc taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatctactat
      301 attaatggac aatgcaagat tccatagaat ggggaagcta gaactcttgt gtgaagagtt
      361 tgggcataaa cttttacctc ttcctcccta ctcgcctgag tacaatctta ttgagaaaac
      421 atgggctcat atcaaaaagc acctcaaaag ggtattacca agttgcaata ccttttatga
      481 ggctcttttg tcttgttctt gtttcaattg actatataaa ttgtctaagc gaaacaaccg
      541 ataagaattg gcacaaaagc gaccgtattt ttgttaccaa tacaggaaaa ccagttcata
      601 gttctatctt gagcaagtct ctccagcgag caaacgaacg ccttaaaaaa ccaattccca
      661 aacatctgtc ccctcacatc ttcagacaca ccactattag catcttatca gaaaataaaa
      721 ttcctttaaa aacaatcacg gacagggttg gtcattccga ctctgaagtc actacttcca
      781 tctacaccca cgtcacaaag aacatgaaag atgaagcaat caatgtactg gataaagtta
      841 tgaaaaagat tttttaaaaa gttttgtccc ttttttgtcc tctaaataca aaaatagccc
      901 ttcggataaa atccgagggg ctagaaacgt tgttaaatca acggccgaac ttttgaattt
      961 catggttcgg gataaaatag ttcactgaac tattttattt tttaaggttg tagaatgatt
     1021 tcaatccacg atattcagct acttcaccaa gttggtcttc gatacgaagc aattggttgt
     1081 atttagcgat gcggtctgta cgtgaaagtg aaccagtctt gatttgtcct gcgttagttg
     1141 caactgcaat atcagcgatt gttgaatctt cagtttcacc tgaacggtgt gatacaacag
     1201 cagtgtaacc agcttcttta gccatttcga tagcttcaaa agtttcagta agagtaccga
     1261 tttggttaac tttgataagg attgagttag cagcaccttc ttggatacca cgtgcaaggt
     1321 agtcagtgtt tgttacgaag aagtcgtcac caacaagttg tactttctta ccaagacgtt
     1381 cagtaagagc tttccaacca tcccagtcgt tttcatccat accatcttca atagtgatga
     1441 ttgggtattt gttaaccaat tcttcaaggt agtcgatttg ttctgcagat gtacgaacag
     1501 cagcaccttc accttcaaat ttagtgtagt cgtaaacttt acgttcttta tcgtagaatt
     1561 ctgatgaagc acagtcaaat ccgataaata cgtctttacc tggtacatat ccagcagctt
     1621 caatcgcagc aaggatagtt tcaacaccat cttcagttcc ttcgaaacga ggagcgaatc
     1681 caccttcgtc acctacggca gtttccaaac cacgtgattt aaggattttc ttaagagcgt
     1741 ggaagatttc agcaccgtaa cgaagggctt ctttaaatgt tggcgcacca actggcaaga
     1801 tcatgaactc ttggaaagcg attggagcgt cagagtgaga accaccgttg atgatgttca
     1861 tcattggagt tggaagaact ttagtgttga atccaccaag gtagctgtaa agtgggattt
     1921 caaggtagtc agcagcagca cgagctacag cgatagacac accgaggatt gcattcgcac
     1981 ccaatttacc tttgttagga gtaccgtcaa gtgcaatcat agcacggtca atagcttgtt
     2041 gatcacgtac atcgtagcca atgatagctt cagcaatgat gttgtttacg ttgtcaacag
     2101 ctttttgtgt accaagacca ccgtaacgag atttgtcacc gtcgcgaagt tcaactgctt
     2161 cgtgttcacc agtagaagct cctgatggaa ccataccacg tccgaaagca cctgattcag
     2221 tgtaaacttc tacttcaagt gttgggttac cgcgtgagtc taggacttcg cgagcgtaaa
     2281 catcagtaat aattgacatt ttttactctc cttatgagtt aaatttttta cacctctata
     2341 ataccttaaa acccctcctt tttcaagaaa aaacgttatc tttgtgcaac ttttccttaa
     2401 ctttataaag taatcgcttt cttttgtctg ttttattcta acttttatga tatactgttt
     2461 tcatgacaga tttatcaaaa caattacttg aaaaagctca tggtgggtta aaaataaatc
     2521 cggatgagca aagacgctat cttggtactt ttgaggaaag agttcttgga tatgtagata
     2581 ttgacacagc aaatagccct cagttagaaa aaggcttttt atttatttta gaaaaccttc
     2641 aggaaaaagc aaagccacta tttgtgaaga tttcaccaac tatcgaattt gataagcaag
     2701 ttttctactt aaaagaagca aaagaaactg atagtcaagc caccatagta tctgaagagc
     2761 atattacttc tccttttggc ctggttattc atagcaatgc accagttcaa gtagaagaaa
     2821 aagaccttcg acttgctttt ccaaaacttt gggaagttaa aaaggaagaa ccagccaaaa
     2881 catccttatg gaagaaatgg tttagctaaa tcttgcacat atttaataag tgcccaatat
     2941 tggcagccgt gcgctccaga tagagactgg catttttcaa actatcttct aaaggttcac
     3001 ttttctccaa aatagaaaag acagcttgga tattttcaaa tggtagggga ggtaaatctt
     3061 cagcaagact accgcaaata gcaataacag gaactccaac aggggttctt tttgcaacac
     3121 ctataggcgc tttcccagca aagctttgac tatcaagtct tccttctcca acaacaacca
     3181 agtcagcatc tgaaactttc ttatcaaagt tgattaagtc caagcaggta tcaattccag
     3241 acacgatact tgcctgagca aaggcacaca aaccagcagc aatccccagt cctccatcat
     3301 tactggccgt gccaccaaca ccgatataaa tatctttaat ccctttagaa atgagatgga
     3361 gaatcaactc tccaatacca caagtttgga tttgaagtgg atttcgtttc tctagcggaa
     3421 tttttccaag accaaccaag tcagctactt caaatagtgc cagttcccct ttttgaaaat
     3481 agcgcatggc ttctttttgt ccaaaagggt ctgtcacttg gatccatttt tcttttaggt
     3541 caagagaatg tcggatagca tctacagtac cttctccccc atcaccaaca gggcagagga
     3601 gacattctac atctgctatc gattgttgga agcctctttt tattgcttca gctacctgtt
     3661 gagctgtcaa gctttcctta aacgaatccg gtgcaattac aatcttcata ttttccctca
     3721 ttctaaacag tcaatcaaag gaagaacttc taaaaaatcc ctcttgtcaa catgatgtgg
     3781 tatttctttt ttgagcattt ctttggaaca aaaggcgatt cctaactccg ccgacttcaa
     3841 cattaataga ttattaaccc catcaccgat tgccaccgtt ctttctttag aaagttttag
     3901 tttctttctc catttttcca gagtttcttt tttgacctgg ggacttataa tttgtccaac
     3961 taattttcct gttaaaagac cttctttgac ttcaagctgg ttggcagtga aataggcaat
     4021 accaagggat tttgctaatc tctccaacta ttggtgtaaa tccaccagac accagaccaa
     4081 ctaggatgcc attcttttgg agaatagaga tgaactctgg gacatttagc gatagatgaa
     4141 ttgagttgaa gacgttatca aagaccaaaa taggaagacc ttccaacaag gacactcttt
     4201 ttcttaaact gctttcaaag accaactctc ctcgcattgc tcgacttgta atctgcgaaa
     4261 tttccgcctc atgacctgcc tctctcccta aaagatcaat cacttcttct aggattaagg
     4321 ttccatctac atccaaaaca cacaagcctt ttacttgaga catcagttct cctctctaaa
     4381 cagcctaaaa atcgtatgaa gtcatcatac gattttatct attaattaac taaactatgg
     4441 tacaagtcaa ggtatgactt gcaggctgta tcccatgaga agtcactctc catagcttgt
     4501 ttttgtaggt ttctccaaat gtctggatgg tttctataca agtccaatgc tgtttggaaa
     4561 gtccaattta accaataagg agatagattg tcaaagctaa agccagtacc gcttccttcg
     4621 attggattga aagcgcgaac tgtatctcgc aagcctccaa cttcatggac caatggcaag
     4681 gttccataac gcatagccat catttgagac aagccacacg gttcaaaacg acttggcatg
     4741 aggaagaggt cacaagcagc gtagatttcc tgagcaagtt tgacatcaaa agtgatattt
     4801 gctgatagct tgtctgggta aatctgagca aaccatgaga aagctccttc aaaggctgga
     4861 tcgccagttc ccaaaagaac aatctgaaca tcttcttgca agatatggtg aagactttcg
     4921 accaccacat caaaaccttt ttgacgtgtc aaacgagaaa caattcccac cagtggaacg
     4981 tctgctctaa caggcaagcc aactctttct tgcaattttg ccttattttt ggctttccca
     5041 gacaaatctt cctgattgaa atgatagtct aaaagagcat ccgtctgagg attataaaga
     5101 tcagcatcaa tcccattcac gataccagat actttaccag actccatttt aagaatctga
     5161 tccaaattac atccaaactg actagtcata atttcatgag catagctagg tgaaacggtt
     5221 gaaacacggt tcgcatagag aatacctgcc ttcatccagt tcagacagtt gttccatcga
     5281 agggtgccat cagcgtaacg ttcaaggcca actccaaaca aatcacccaa cattccttct
     5341 gaaaattgtc cttggaattc taaattatga atggttaaaa ctgtttcaat gtcctcatag
     5401 gcttgaatcc aacagtattt ttccttcaac aagaaaggaa tcatagctgt atggtagtca
     5461 tgaacatgga gaagatcagg aataaagtca atcctttcca tagcctcaat ggcagccagt
     5521 tggaaaaagg caaagcgttc tccgtcatca aaatcaccgt aaacatgacc acggaagaaa
     5581 taatattgat tgtcaataaa gtagaaggtt acaccattta atactgtttt cttaattcca
     5641 caatactgtc tacgccaacc aacgctcacc tcaaaatgaa gcacatcttc aatctgattt
     5701 ccaaatttag cctctaccat atcatagtag ggtaaaatca ctgcaacttc gtgcccagct
     5761 tttaccagtg attttggaag agcgccaatg acgtctccca aaccacctgt ttttgaaaag
     5821 ggtgcaccct ctgctgctac aaataaaatt ttcatgaatg aatatcctct gttactttag
     5881 cacctttctt aaccacaact ggatgttctg cagttcctcg aatcacaaca ccaagctcaa
     5941 cttcaacccc tttgtccaag atagcatatt cgacctgagc cccttctcca ataacaacac
     6001 gagggaagag caggctatct ttaaccaagc tatccttatg gacatgaata ttacgtgata
     6061 gaacagaatt agctacttga ccttcaataa tactaccaga ggcaaactga gaagtgctta
     6121 ccttagatgt attagcatag taagttggct cttcgttttt gacctttgta taaatctttt
     6181 ggtttggtga gaaaagagaa tagaattttt gtgattcaag catatcgata ttcgcttgat
     6241 aataagattt aacagagtga atattggcta gatagcccgt gtactcgtag gcaaaagctc
     6301 cctcttttac agccaaatcc cgtaaaacat agcgcaattt ctctggatgt tcttttttag
     6361 cttcttcttc caagtgttca atcaaccaag gtgtatcaac gacaaagata tctgtagaca
     6421 tattgaacgt ttcagctgtt gacttgctat caaagagttt atgagaaaga acatggtctg
     6481 tttcatctac atccaagatt gcatttactt ctgaaatatc tttcttagct agttttttat
     6541 aaactacagt gataggctct tttgttgtac tatgtaggtg gaaaacttgg ttcaaatcaa
     6601 tgttaataag aacatcgcag ttgagggcaa ccgtttggtt tgagccagaa cgtttcaaat
     6661 aagtaagaag ctgttggtag tattcttttc caactgtact actttctaca cgggtattgt
     6721 aaattcctag atagtaatgg ctaagaaggg ttgataagcc ccactcgcgc cctgaacgaa
     6781 tatggtcaaa gactgagctg atattatcct gctggaaaat accaaagaca ctacgaacac
     6841 ctgcattagc aaggcttgaa agtgggaagt caatcaaacg atatttccca ccaaatggca
     6901 aacttgctac tggacggtgg tccgtcaatg tcgacatatc gtgaaaacca actgtatttc
     6961 ctaaaatggc agaatattta tcaatcttca tctgttgcta cccccactac ttcattatat
     7021 cctacaactt gtacttcatc tgttccatca atttcgacac cgtcagaaat aatcgcacct
     7081 tcaccaataa tggcacgttt aatcttagct ccttgaccaa tgatagctcc actcatgata
     7141 actgaatcaa ggacttccgc tccttcgcga acttgcgcgc ctgttgaaag gatagaatgt
     7201 ttaacagttc catcaacgaa acatccgtct acaactaatg agtcttccac atgagcattt
     7261 gccccgagga agtttggtgg tgaaatcaag tttcttgagt aaatcttcca ttgacggtta
     7321 cgactatcca aggcattttc tggagaaata tactccatgt tcgcttccca aagtgactca
     7381 atagtaccaa catctttcca ataaccacta aattcataag cataaacact ttcacctgac
     7441 tcaaggtaat ttggaatgac atttttacca aagtctgaca tgccaacctt gctcttttca
     7501 gcagcgacta acatattacg aaggcgttgc caatcaaaaa tgtagattcc catagaagct
     7561 tttgtagatt taggttgagc tggtttttct tcaaattcaa caatacgatt gttagcatct
     7621 gtgttcatga taccaaaacg gcttgcttct ttaagaggga cgtctaaaac tgctactgtc
     7681 aagctggcat tattatcctt atgagactgg agcatatcat catagtccat tttgtagatg
     7741 tgatccccag acaaaatcaa gacatactca ggattgacac tgtcgatata gtcgatattt
     7801 tggtaaatag cgtgactagt cccctcaaac caacgatttc cttcacttgc agaataaggt
     7861 tgaagaatag agacacctga attaataccg tctagtcccc agcttgaacc attcccaata
     7921 tggttgttga gagcaagtgg ttgatactgt gtaacgaccc caacattatg aatccctgag
     7981 ttggcacagt ttgatagggc aaagtcaatg atacggtagc gcccaccaaa ttgcacagct
     8041 ggttttgcga tgctttgagt gagtttaccg agacgagttc cttgcccacc agcaagaatc
     8101 aaagctaaca tttcattttt cattttctac tcctttttgg tttttatttg tgacggtttt
     8161 agtagatttc aagcgacgtt tgattttcca tacacttgct cccatagccg gtagggtaaa
     8221 ggttaaggtc tgctcataat ctttccatag tccttcttgc gtttgaacag tttgattatg
     8281 ttctttccaa acgcctcccc actcttccaa ctcagtattc catacttctt cgtaaattcc
     8341 tgcaacgggt agtccgattg taaaatcttt ccgctcaaca ggtaccatgt taaagataca
     8401 gactaacatt tctccctttt tacccttacg aataaaggaa agaacactct ggtctcgatt
     8461 atccgcatca atgatttcaa taccatcata gctggtatcg atttcccaca gacagcgatg
     8521 atctttgtaa aactggttta gctgagaagt gaaatacttc atcttagcat tcattgggtc
     8581 ttctaggtta gaccattcca actgttcttc agatttccat tctaggaatt gaccgtattc
     8641 gctacccatg aagagcaatt tcttaccagg atgacaaatt tggtacgtat agagattgcg
     8701 caagcctgcg aattgattgt aacgatctcc ccacatctta tgcatcatac tcttcttgcc
     8761 atgaaccact tcatcgtgcg agaatggcaa gagataattc tccttgaaaa catacataaa
     8821 gctgaaagtc accaggttaa agtcatattt acgatagatc ggatcttctt cgtagaaacg
     8881 gaggatatca ttcatccagc ccatgttcca tttgtagtca aatcctagac caccaatctc
     8941 tttcattccc gtaatcttgg tcgcagacga actttcttct gcaatcatca tcacatctgg
     9001 atattctaac ttaataacct cattcaagcg ctgaaggaaa taataacctt
//
LOCUS       DBEJAQ010000064         8813 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.064, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000064 DBEJAQ010000000
VERSION     DBEJAQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.064"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            158..1177
                     /gene="galE"
                     /locus_tag="ACL297_001865"
     CDS             158..1177
                     /gene="galE"
                     /locus_tag="ACL297_001865"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGR0072071.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1179..2132
                     /locus_tag="ACL297_001866"
     CDS             1179..2132
                     /locus_tag="ACL297_001866"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGR0072072.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(2194..2400)
                     /locus_tag="ACL297_001867"
     CDS             complement(2194..2400)
                     /locus_tag="ACL297_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGR0072073.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            2452..2928
                     /locus_tag="ACL297_001868"
     CDS             2452..2928
                     /locus_tag="ACL297_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HGR0072074.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            2937..3608
                     /gene="cmk"
                     /locus_tag="ACL297_001869"
     CDS             2937..3608
                     /gene="cmk"
                     /locus_tag="ACL297_001869"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGR0072075.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            3739..4077
                     /locus_tag="ACL297_001870"
     CDS             3739..4077
                     /locus_tag="ACL297_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGR0072076.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            4080..4709
                     /locus_tag="ACL297_001871"
     CDS             4080..4709
                     /locus_tag="ACL297_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HGR0072077.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            4949..6100
                     /locus_tag="ACL297_001872"
     CDS             4949..6100
                     /locus_tag="ACL297_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGR0072078.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            6413..7162
                     /gene="truA"
                     /locus_tag="ACL297_001873"
     CDS             6413..7162
                     /gene="truA"
                     /locus_tag="ACL297_001873"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGR0072079.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            7152..7925
                     /locus_tag="ACL297_001874"
     CDS             7152..7925
                     /locus_tag="ACL297_001874"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGR0072080.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            7903..8364
                     /locus_tag="ACL297_001875"
     CDS             7903..8364
                     /locus_tag="ACL297_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGR0072081.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            8591..8800
                     /locus_tag="ACL297_001876"
                     /pseudo
     CDS             8591..8800
                     /locus_tag="ACL297_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2507 a         1632 c         1974 g         2700 t
ORIGIN      
        1 tacccatggc aaggcgacgt tgacgtggtt tgaattggat tttcgaagag tataagcatt
       61 ttactgaaat tttatcaggt agtttaggat ggcaaatgac attccctgtc aaaaatggta
      121 aaataagaaa aaataatctg agaatcgagg aaaaaagatg caagaaaaga ttttggtaac
      181 tggtggtgcc ggttttattg gaacccacac tgttattgaa ttgatccaag caggccatca
      241 agttgttgtg gtggataatc ttgtcaatag caatcgtaag agtttagaag ttgttgaaag
      301 aatcacagga gttgagattc ctttctatga ggcagatatt cgtgacacag ctaccctcag
      361 agatattttc aagcaggaag aaccgactgg tgtcattcac tttgctggtt tgaaggctgt
      421 tggcgaatca acacgtatcc ctcttgccta ctatgacaac aatatcgctg gaactgtcag
      481 ccttttgaaa gccatggaag aaaacaactg taaaaacatc atcttcagtt cttctgcgac
      541 agtttacggg gatccgcaca cagtgcccat cttggaagat ttcccacttt cagtgaccaa
      601 cccatacggt cgtactaagc tcatgctaga ggaaattttg actgatattt acaaagcaga
      661 ctcagaatgg aatgttgtct tgcttcgtta ctttaaccca atcggagccc atgagagtgg
      721 tgatttggga gaaaatccaa acggtattcc aaacaatctc ttgccatatg tgactcaagt
      781 agccgttgga aaattagagc aagtgcaagt gtttggagac gattacgata cggaagatgg
      841 aacaggtgtt cgtgactata tccacgttgt cgatttggct aagggtcacg ttgcagcttt
      901 gaaaaaaatc caaaaaggtt caggactaaa cgtttataac cttggaactg gtaaaggtta
      961 ctcagttctt gaaattatcc aaaacatgga aaaagcggtg ggccgtccta ttccttaccg
     1021 catcgtagaa cgtcgcccag gtgatatcgc tgcctgctac tcagacccgg caaaagctaa
     1081 agcagaactc ggttgggaag cagaactcga catcacccaa atgtgtgaag acgcatggcg
     1141 ttggcaaagc aagcatccaa atggatttga agactaagat gatgatttca atcatcgtcc
     1201 cttgtttaaa cgaagaggaa gtacttcctc ttttttatca ggctctggaa gctttacttc
     1261 cagatttgga aacagaaatc gagtatgtct ttgtcgatga tggatcaagt gatgggacct
     1321 tggaactctt aaaggcctat cgggagcaaa atccggcagt ccattatatt tctttctctc
     1381 gaaattttgg caaagaagca gccctttatg caggcttgca atatgcgaca ggagatttgg
     1441 tggtggtgat ggatgcagac ctccaagatc ctcctagtat gttgtttgag atgaaaaatg
     1501 tactagacaa aaatgtagac ttggactgcg ttgggacacg gagaactagt cgggagggag
     1561 aacccttctt tcgcagtttc tgtgctgttc tcttttatcg cctcatgcaa aaaatcagcc
     1621 cagtagctct gccgtcgggt gtccgtgatt ttcgtatgat gagaaggtct gtagtcgatg
     1681 ccattttaag cttgaccgag tccaatcgtt tttcaaaggg gctcttcgcc tgggtgggtt
     1741 tcaaaaccta ctatttggac tatccaaatg tcgaaaggca ggctggcaag accagttgga
     1801 gttttaggca actctttttt tactccattg aagggattgt taatttttca gatttccctt
     1861 tgactatagc ctttgtagct ggtctcctat cttgttttct ttctctgctg atgacctttt
     1921 ttgttgtggt tcggaccctc attttgggca atccgacatc tggttggacc tctctgatgg
     1981 ctgttattct ctttcttgga ggcattcaac tcttgaccat tgggattctc ggtaagtata
     2041 tcagtaagat ttatttagag actaaaaaaa gaccacttta tcttatcaaa gaaaaaagtg
     2101 accttcctga ttttacagaa aaaataaagt gaaaagacta taattttaca tggaaatgtg
     2161 ctaaactaga gggagtggat tatcgccatt gatttaggct tcctggtttc caatcagggc
     2221 gcgagttggg caatttttaa cagcctctaa gacatcctga ctaggagaaa tttctttttc
     2281 cagttggtca gggtcatcgt aaaaacgcac gattccatta tcgtggtaat caaataaatc
     2341 agaataagtt tggcaaagtc cacaggcgat acatcgttca ggtataagtg tgattttcat
     2401 gttcatattt atattgtaat aagaaaagta aaaaaaaaca aggagtaagg tatggcaaaa
     2461 gaaccgtggc aagaagatat ttatgatcaa gaagaatcaa gagcagggcg tcggcatcga
     2521 aaccacggag gggctgatag gatggctaat cgtattttga cgatcctagc tagtattttc
     2581 tttgtaattg tggtggtgat ggtcatcgtt ctcatctatc tatcatcggg ggggagtaat
     2641 cgcacagcag ccttaaaaga ctttcatgat tctgatgcaa gtgtagtaca aatctcatct
     2701 tcaagcagtt ctcagcctga gcagagttca gagccagaat ctacttctag tagttcagaa
     2761 gaagctgcta atcctgaagg aacgattaaa gttctcgcag gagaagggga agcagctatt
     2821 gccgctcgtg caggaatctc cattgctcag ttagaggcct tgaatcctgg gcacatggct
     2881 acaggatctt ggtttgctaa tccaggtgat gttataaaaa taaaatagga gccgatatga
     2941 aaacaattca aattgctatt gatggtcctg cttccagcgg taagagtacg gtcgcaaaga
     3001 ttattgctaa ggattttggg ttcacctacc ttgatacagg agctatgtat cgtgcagcga
     3061 cctatatggc tcttaagaac caattaggag ttgaagaagt cgaagccctt ctagccttgt
     3121 tggaccagca tccaatcagc tttggacgtt cagaaactgg agaccagctt gtttttgtag
     3181 gagatgtgga tattacccat cctatccgtg aaaatgaagt gaccaatcat gtttctgcta
     3241 ttgcagcaat tcctgaagtg cgtgagaaac tggtttctct ccaacaagaa attgcccagc
     3301 aaggcgggat tgtcatggat ggtcgcgata ttggaactgt tgtattgcca caagcagaat
     3361 tgaaaatttt cctagtagct tctgttgatg agagagcaga gcgtcgttac aaggaaaata
     3421 ttgccaaggg aattgaaaca gaccttgaaa ccctaaaaaa ggaaattgct gcgcgtgact
     3481 acaaggatag tcatcgtgag acttctcctc tcaaacaagc agaggatgct gtctaccttg
     3541 atacaactgg tttgaacatt caagaagtag ttgaaaaaat caaagcagaa gctgaaaaaa
     3601 gaatgtaaat gtagaaaagc cgatagattg atatcggttt ttttctaaat ttgtcaaaat
     3661 gctgatttta gggtataata gttgctgttc gagaaatttt gattttcaaa gaatagtgaa
     3721 tgatgataag gagaaaccat gaacaattta ccaaattgtc caaaatgtaa ctcagagtat
     3781 gtctacgaag acggtgccct actggtttgc ccagagtgtg ctcatgagtg gaatcctgct
     3841 gaagttgcag aagtagaaga aggtcttgtc gctatcgatg ccaacggaaa taaattgact
     3901 gatggtgata cagtaactct catcaaggac ttgaaagtaa aaggtgcgcc aaaagatttg
     3961 aaacaaggga cgcgcgtgaa aaatatccgc atcgtagaag gcgatcacaa tatcgactgt
     4021 aagattgatg gttttggtgc catgaaactt aaatcagagt ttgtgaggaa gatttaagca
     4081 tgtcaaaaca ctataaactt gtattttata gccgtatctt cttgtttcta gcggctttta
     4141 cgggagttta tcttgaaatt actaggcatg gtggttttgg gatgcttctc tattacacgg
     4201 ttctgtccaa cctcttggta actattttta ccctttatct tctaaaggtt atgagccgtg
     4261 taggtgaaaa ttggcaaagg ccaagtctct tgcgcttaaa aggtggggtc accatgagta
     4321 tcatgattac ctgtgtgatt taccatttcc tcttggcgcc cattgcgact aatttctata
     4381 ccctagaaaa tttcctttgc cactatatcg ttcccatctg gtttttagcg gataccctct
     4441 tttttgacaa acagggtcaa tacaagattt gggatccagc agtgtggacc attttaccct
     4501 ttctgtatat gatgtttgct ctttttaatg gcttggttct aaaactcaat attccaaatg
     4561 ccaaggataa tcctttccct tacttctttt tgaatgtgaa caagggttgg aatgttgtgt
     4621 ttaagtggtg tctgattatc tttgttgcct atatggtagc aggatttatt ttctacttta
     4681 tcaagcaaat caagagaaag tcatcctaat actcttcgaa aatctcttca aaccacgtta
     4741 gtttccatct gcaacctcaa aacactgttt tgaacaacct gcggctagct ttctagtttg
     4801 ctctttgatt ttcattgagt atgagataca agggattcat tttcgaattt aagaaggagt
     4861 ctacaaacca atgaggcttc ttcttttctt gcttatttga taactatcaa aaaagtagaa
     4921 gattaagaga aatatagaaa gagatttcat gaaacaattt ttagaacggg ccagcatttt
     4981 ggctctctcc ctcgttttga ttacctcctt ttccatttcg agtgccctac cagccatgtt
     5041 tgactattat cagggttatt ctaaggaaca aattgagctc ttggtgagct tgccttcctt
     5101 tggaatcatg atgatgttac tgctaaatgg tttcttagaa aaaatatttc ctgagcgctt
     5161 acagattagt ttgggcttgc tgattttatc attgagcggt acagctccct tctggtacca
     5221 agcctatccc tttgtctttg gaacacggct tctctttggt ttgggtcttg ggatgatcaa
     5281 tgccaaggcc atttctatta tcagtgaacg ctaccaagga aaaaggcgaa ttcagatgtt
     5341 agggctacgc gcttctgcag aggtcgttgg agcttctctc attaccttgg ccgtcggtca
     5401 gttgttggcc tttggttgga cagctatctt tctagcctat agtgctggat ttttggtgat
     5461 gcccctttat ctgctctttg tcccttatgg aaaatcaaag aaagaagtca agaaaagagc
     5521 gaaggaagca agtcgtttaa ctcgagaaat gaaaggcttg atttttacct tagctatcga
     5581 agcggcagtt gtagtttgta ccaatacagc tattaccatc cgtattccaa gtttgatggt
     5641 ggaaagagga ttgggggatg cccagttatc tagttttgtt cttagtatca tgcagttgat
     5701 cgggattgtg gctggggtga gtttttcttt cttgatttct atctttaaag agaaactgct
     5761 cctctggtct ggtattacct ttggcttggg gcaaatcgtg attgccttgt cttcatcctt
     5821 gtgggtggta gtagcaggaa gtgttctggc tggatttgcc tatagtgtag tcttgacgac
     5881 ggtctttcaa cttgtctctg aacgaattcc agctaaactc ctcaatcaag caacttcatt
     5941 tgctgtatta ggctgtagtt tcggagcctt tacgacccca tttgttctag gtgcaattgg
     6001 cttactaact cacaatggga tgttggtctt tagtatctta ggaggttggt tgattgtaat
     6061 ctctatcttt gtcatgtacc tacttcagaa gagagcttag gattgattcc taagtttttc
     6121 ttttgatact caatgaaaat caaagagcat agttgattga gtttggaata gtacgctgta
     6181 gctgctaaaa tatttctaga aattaatttg acttttctaa tagagttatt catatcttat
     6241 ttcaatctac tatagaaagc tagccgcagg ttgctcaaaa tagtgttttg aggttgcaga
     6301 tggaaactga cgtggtttga agagattttc gaagagtatg agatttttat acccatacaa
     6361 tatttgtttt aggtccttgt ttacagacta atttcttgat aaaatagagg ttatgacgag
     6421 atataaagca actatttcct atgatggtta tgcctttgct ggctttcagc gccagtctca
     6481 tgcgcgtagc gttcaggaag aaattgaaaa aaccttgacc agattaaata aagggcaaac
     6541 cattactgtt cacggtgctg gtaggacaga tagtggggtt catgccctgg gacaggtcat
     6601 tcattttgac ctgccttatc agatggatga ggagaaactc cgttttgcct tggataccca
     6661 gtctcctgaa gatattgatg tgatttcgat tgagcttgtg gcagatgatt ttcattgccg
     6721 ttatgccaaa catagcaaga cctatgagtt tattgtggat agaggacgtc ccaaaaatcc
     6781 tatgcgccgt cactatgcca cccactttcc ctacccactc gatgtggaac gaatgcagat
     6841 tgcaatcaaa aagctagagg gaacccatga ttttaccggt tttacagcct ctgggactag
     6901 tgtagaggat aaggttcgca ccatcacaga agctagttta atagtcgatg agacaggaca
     6961 atttttgacc tttacctttt caggaaatgg tttcttgtat aaacagattc gcaatatggt
     7021 ggggactctt ctcaaaatcg gtaacaaccg catgccagta gagcagattg atctcatctt
     7081 ggagaagaag gacaggcaac ttgcaggtcc cactgcagca ccaaatggtt tgtatttaaa
     7141 ggagattcgt tatgaagaat aatcgtattt tagcactttc tggaaatgat atttttagtg
     7201 gtggtggact gtcagctgat ttggctacct ataccttgaa cggcttgcat gggtttgtag
     7261 cagtgacttg tttgacagcc ttgacagaaa aaggatttga agtctttcca actgatgata
     7321 ccatttttca acatgaatta gatagcttac gtgatgtgga atttggggga attaagattg
     7381 gtcttctccc tactgtcagt gtggctgaga aggccttgga ctttatcaaa caacgcccag
     7441 gagtacctgt ggtgttggat cctgtcttgg tctgcaagga aacgcatgat gtagctgtca
     7501 gtgagctctg ccaagagttg attcgctttt tcccttatgt cagtgtgatt acgcctaatc
     7561 tcccagaagc agaattatta tccggtcagg aaattaaaac cttggaagac atgaaaactg
     7621 cagcgcagaa attgcatgat ttaggagcgc cagcagtcat tatcaaggga ggcaatcgtc
     7681 ttagtcagga caaggctgtg gatgtctttt atgatggaca gacctttact atcctagaaa
     7741 atccagttat ccaaggccaa aatgctggtg caggttgtac ctttgcctct agcattgcca
     7801 gtcacttgat taaaggtgat aaacttttgc cagcagtaga aagctctaag gctttcgttt
     7861 atcgtgctat tgcacaagca gatcagtatg gagtaagaca atatgaagca aacaaaaaca
     7921 actaaaatcg cccttgtatc cctattaacc gccctttctg tggttctagg ttatttctta
     7981 aaaatcccaa cacctacagg aattctaact cttttagatg ctggtgtctt ctttgcggcc
     8041 ttttactttg gtagtcgtga aggagcggta gtcggaggac tagcaagttt cttgattgac
     8101 ctcttatcag gctaccctca gtggatgttc tttagcttgg tcaaccatgg cttgcaggga
     8161 tttttcgcag gatttaaagg aaaaagtcag tggttaggcc ttattttagc aactattgcc
     8221 atggtaggag gctacgcctt gggttctact ttgatgaatg gctgggcagc agccctccca
     8281 gaaattctac caaatttcat gcaaaatatg gtagggatga ttgtaggatt tattcttagt
     8341 caaagtatca agaagattaa gtaatactct tcgaaaatct cttcaaacca cgtcagcttc
     8401 catctgcaac ctcaaaacac tgttttgagc agcctgaggc tagctttcta gtttgcactt
     8461 tgattttcat tgagtataaa gaggttgagt aaaaagtatt tcaaataaga aaaacgcata
     8521 gtatcaggtg ttgaatgaac tgccccccaa aagttagaca gaaaaaatct aacttttggg
     8581 gtgttttatt atgaaactaa cttatgatga taaagttcag atctatgaac ttagaaaaca
     8641 aggatatagc ttagagaagc tttcaaataa atttgggata aacaattcta atcttaggta
     8701 catgattaaa ttgattgatc gttacggaat agagttcgtc aaaaaaggga aaaatcgtta
     8761 ctattctcct gatttaaaac aagaaatgat tcataaagtc tgacatgaag gct
//
LOCUS       DBEJAQ010000065         8165 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.065, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000065 DBEJAQ010000000
VERSION     DBEJAQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.065"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(173..1588)
                     /locus_tag="ACL297_001877"
     CDS             complement(173..1588)
                     /locus_tag="ACL297_001877"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGR0072082.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1601..3439)
                     /locus_tag="ACL297_001878"
     CDS             complement(1601..3439)
                     /locus_tag="ACL297_001878"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGR0072083.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3457..4296)
                     /gene="licT"
                     /locus_tag="ACL297_001879"
     CDS             complement(3457..4296)
                     /gene="licT"
                     /locus_tag="ACL297_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGR0072084.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4596..7154)
                     /locus_tag="ACL297_001880"
     CDS             complement(4596..7154)
                     /locus_tag="ACL297_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGR0072085.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
     gene            7702..8165
                     /locus_tag="ACL297_001881"
                     /pseudo
     CDS             7702..8165
                     /locus_tag="ACL297_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2698 a         1684 c         1151 g         2632 t
ORIGIN      
        1 ccaatctatc ctaagttaac tatttataat cggctctctg taacaaaatt aaattaatta
       61 atccccctat aaaataaaga ggtttagaat ttgatgtctt ccaaacctct ttatttcata
      121 tttaatgaga tgctgcctta accgtgataa tatctaatca tcaataaaca aactatttga
      181 ataaggattc tccatttgat tcaatcactc ctttatacca agtaaaggac attttcttat
      241 atcgattgaa tgtgccattt ccatcatcat ttcgatcaac ataaatgaga ccgtacctct
      301 tagaaagctg tgcagtggac atagaaacgc agtcaataca tccccaagac gtatagccca
      361 taatttccac accatcttgt agagcttcag ctacttgcaa taaatgttct ttcatatact
      421 gaattctata atcatcttgg actgttaagt tattaaattc atcttttatt agttgatctt
      481 tagcacctaa tccgttttcc actataaata atggaatttg ataacggtca taatatctat
      541 ttaaaattat acgtagtcca attggatcaa tttgccatcc ccactcagaa gactctaaat
      601 aaggatttac taaaccacca ataatattcc cttctcctga attatactgt gttggaagag
      661 cagattgagt cacactcatg taatagctaa aggataaaaa atctacagta taatttttta
      721 ataactctgc atcttcagct gcaaactcta tgttaatgtc attttcctta aaatatcttt
      781 ttgcataatt cggataataa cctctaacat gcacatctga aaatagataa tttagattct
      841 catactcatg agtcgcccat acatcttttg gatttggagt cattggataa gctggcatag
      901 ctaataccat acaccccacc ttaaactctg aattaatctc acgagcaatt tttgtaacca
      961 aactcgaggc gactaattca tgatgtatag cttgatataa ttcttgtttc gaaagattct
     1021 ccttaggtat atctattcct ccactagtaa acggtaattc caaaacagag tttacttcgt
     1081 taaatgtaag ccaatattta actttatctt tatacctttc taaaactgtt cgagcaaatt
     1141 tttcataaaa atgaatcatt ctcctatcaa tccatccatg atattttctt gctaaatata
     1201 atggagtctc atagtgtgaa agagttacaa gtggttctat cccgtgagca tgtagttcat
     1261 caaacaattc atcataatat ttcaacccag cttcgttagg ttcttcctca tctccttttg
     1321 gaaaaattct actccatgca atagaagtac gaaaaacatt aaagcccatt tcagaaaaca
     1381 aggatatatc ttccttatat ttatgataaa aatcaatacc tatcaatttt aagttatctt
     1441 ctgtaggatt ttctgttgct tctcctaatc cacctttggg taacacatcc tgaactgata
     1501 agcccttacc atcttcatta tatgctccct ctacttgatt agctgcaaca gctccacccc
     1561 aaagaaaatc atctggaaaa atggtcataa ctttcctcca ttataatatt accagtaatt
     1621 ccttagaatg ctcgattgtc tgattattag gtaatactaa tacatctaga aaatcattgg
     1681 tattcgttac aattactggt gtaactgttt cgtagccttt agtcttgatt aaattcaagt
     1741 ccatttcaaa aatcaactga tttttgaaaa ctctgtctcc ttcttctaca tgactaataa
     1801 aaccttgacc ttttagctca acagtatcta atccaatatg aattagtaac tcaacaccct
     1861 catcactctt caatccaatt gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa
     1921 atggtgcata aaccttacct tcacttggaa taatcgctac tccgtctcca attagtttat
     1981 ctgaaaatgt tttatcctgg acatcgctta acggaatgat ttctcctgat ataggagaaa
     2041 atatcatttt tttatttgaa actccagctt taacttctaa attgctagaa ctctcttctt
     2101 catcgattcc aaatatataa gctaatacaa aggtaataac aaccgaaata accgccacaa
     2161 ttaaagcatt tacaatattt gatggcacat cagaataaat aaattgaggc aacgctatca
     2221 aagatgggac agcaaataga tacgctttaa cactagtaag acctgcaaat aatcccgcta
     2281 atccaccacc aatcatagct gcataaagcg gttttttata ttttaaagtc acaccatata
     2341 atgcaggttc ggtaatccct gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt
     2401 ttgtattatt atttttactc tttaatgcaa cagccatcga agcagcccct tgagctaagt
     2461 ttgaccctaa cattgctgga agaattaata cgtctggagt agcaatagat gccgccaaaa
     2521 aaataggtgc aaaagcccaa tgcattccag tcataacaat aaatggcata atagcaccaa
     2581 gaatagctaa tgtaagccat ccagctacac catacatttg cccaactaga tttgataatc
     2641 cttcaccaac aattactcca ataggcccga ctacaactaa ggcaatacag cttgatacta
     2701 ataatactag cgtaggttgc aaaaaactct tagtaacagc tggtgttaat ttagcaatca
     2761 ttttttcaat atatttcatc aaccaaacca taataagaat tggaacgact gacgaaccat
     2821 aactagctgg tgtcacaggt acaccaaata aactaagagg attccctgat tgcaccattt
     2881 gaacaaaatt tggatggaga agtacacctg ctacagacat agctaatgta gatgttactt
     2941 ttaatttttg tgatgcagaa taagctaata acagcggtaa gaaataatat ggagcatccc
     3001 caaaaaatgt caaaaaagca atagtctgag aatctgattg caatatacca agcattggta
     3061 aaataattac caagactttc agcatacctc cccctaacat tgctggaatg attggagtca
     3121 tggaaccagc gatatactca atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa
     3181 ctgaatcgga ttcaaaattg ccaagtttaa cgaattcttt ataataatta gctacatcat
     3241 taccaagtat aatttgatat tgtccattct ttttcataat acctattaca cctggtatct
     3301 tcttcacatc atcatcattg actaaatttt catcttttaa ttctaatctt aaacgtgtta
     3361 cacaatgggt aactctattg acattttttt cacctccaat tacatcgagg attttttgta
     3421 ccgtatcttt ataactcatg gtattctcct attctattaa tctaaatttt ttgttaagcg
     3481 acgaatatga gccatcaaat aaactaattc actagaagtc agcaaataat tgtactccgt
     3541 ttgtataaac attgccacct gttcaccaca ttcatattct ctaggatatt tatttttcat
     3601 taatgctaac aagtcttcat catcatcgtc acaataagtt gtattttcaa ccaagcgatg
     3661 ggcaaataat tttatatgag ttatgaatct ataataatca atagaatctt ggttaaactc
     3721 agtacaatag aaatcttgaa tgattttctc aattcccata attatttcag tgattttata
     3781 cgactcttga aaattatttt ctaaattagc attaacaaaa tgtagcgcta tgaatgcagc
     3841 ttcgtcaatt ggaagttcaa tacctaactc tttttttatt atttgtaaag cccttttccc
     3901 aagttcaaat tcttctttat aatatttcga tatttcccag cgtaggggat tcgaaataat
     3961 aatcccttct ttataacgtt ctatgctcga agaaatatga tccgttaaat taatatagat
     4021 aatttcatca aaattattac ccaatgttat tttacctagg ttaattattt tttcggtgat
     4081 gtacaccaac tccaaaggaa cgttaataaa catctctgta aaacgggtca tattatctct
     4141 atttttgaga aaaaaacttt tctcaatatc aattggattt actttatctc cagtttttct
     4201 tccaaaagct attccccttc caaaaagaag aatatcgata tcttttttac tttgcgcaat
     4261 tacggcattg tggtttagta ttcgttttat gatcataaaa tttccctttt aaaataaaag
     4321 gaacctactt aaaaacatca agaaacttct tctgatgtga ttaaataggt tcctgctcac
     4381 ttgtattagt gataacatcc ttttatttca atttttaatg gtgacatgaa gcttaaataa
     4441 ttaataactc tgtaaccgtt tttattttat gatatttcaa tctatttgtc aagtacttat
     4501 aaaaattttt ttattttgtg tgattcgaat gtgataatgg caacagtctc actaaagaaa
     4561 tgaaaatcat acatagaaat ttttaaaaat tttctctatc gtaaacctgc cactagttca
     4621 gaaactatac tagctgattc caaacgtaat atggtcatct catctacctt ccctaataat
     4681 ggcattgcac cataccaaac aatagtctta ttcaccaaaa gaataggcgt tactattttt
     4741 gattgagtaa aagacacttt aattttctgg ctcttcaact ggtctagcca tgctttattt
     4801 gcaaccgtct ctggtataca tatctcaatt tgactaatcc taaattcttt tagaaaattc
     4861 atcagttttg tctggtgcac ataaggtaaa ataagcagac attcctgtct ttcccctgct
     4921 aagtcctctc tcaacacctt ctcatatcta ctatcaacat aaacgaattg tttctcctca
     4981 ccctcgatga cacgataatc catctttcga taagctactt gtcgtttctg aaacatcttt
     5041 tctaaataag gaacatgaat atccacataa tcgaaaatac gcaccaaaga cttatccttg
     5101 tagtttctat gaatccgacc tgcatactga attaaattat ttttccaaga aaagggtgct
     5161 gccaagataa gcgtgtccaa ctgaggtaag tcaaaacctt cgccaatgta ttttccagta
     5221 gacaacaaaa caaacccttt atctaactgt tctaacgtct ccagtaaact cgttctttct
     5281 cggactttgg tttttccgct aataatgtaa cagccatcaa cctctttctc tttcaataac
     5341 ttttcaaaga catctatctg ttgaattcga ttaactaaaa ccaagatatt ccgtccttct
     5401 gccacttggg ctagaatatc cttgagaatc aactgattcc tcgctgagtc tgtagcaatc
     5461 caatcactga gctgtataaa attacttgct ttggtctttt caatctccaa atgaccaaaa
     5521 gaagtgaatc ttaattgcaa ttgccgttta aaatccgttt ccctcttatc agcagtatgg
     5581 agtatctcac caattctctg aaaaacaata ggctcatgac cattcttacg ctcaggcgta
     5641 gccgtcaaac cgtaaagata cttccctcta aactgagcaa caactttttc aaacatcaag
     5701 gcagagacat gatgacactc atccacaatc atcatctcat actcatccaa aagactttga
     5761 ctattttcca acttaaatag agattgaatc ataacgacat caaccagttt actcagccat
     5821 ttcttagtcc caccgtactg tccaacatag ccgattacct tttcacgacc tgatgccgta
     5881 taacggatag cctcctcctc ttcgaaagtc aaaaagcagt ttaagcgatc tagccattgg
     5941 tctaagagtt gcttattatg gactagaata attgtttttg ttttccgttc agagataaga
     6001 gcagcaccta aaacggtctt cccaaaacca gtctccgcat gaagtaaacc attttcttta
     6061 gaagtcatat ctgacagggc taactcttgc tcaaaagtga gttctccctt aaattccacg
     6121 ctaatagacc tttgtacctt tctcctatct tccacagata cctgcttaaa tttatcttgc
     6181 aatggataca gcaaacctct tggcaaccat aaataatgat cggattctcc aaataaatac
     6241 attctctcag gaatttgata ggttggctgt cgcatagcct gctttaaata aaattcggga
     6301 ttagaaaatg aagccatatt tttcaagaaa aacagtgtct tgggtgtcac agacgatttt
     6361 tcaagttgga tcatattgga taagacgatc ttcaactcct tgtccaattc ttgcttgcct
     6421 aactcctctt ggattaacag tgccacttta gcagttgaaa tcttctgaat ttcttgtaga
     6481 tacctccatt gatcttcata aggctgaaac tgttcatcca caaagaccgt tcgcccttga
     6541 tgataagctt ctccttgaaa aggaagggaa attaaattat caaatccacc tttaggaaga
     6601 acatcctgat ttggaaacat gcgatcaaaa gaatcaaagg acagttgcat actttcctgc
     6661 atagccaatt ctaatagttt ctttccaaac aggcgagcct ttcgactcag aatcgcttcc
     6721 tcaaagaaga accaaatatg gagtccgtaa cccgaacgag aaatctctaa atgggcttcc
     6781 atctggcgtt ccctggctat tcttcgaatg gttaaaccag cttctttcca atctccttca
     6841 tccaaatcca gtaccaaaaa ataacagcta tcatctaagt gcataggaaa gataccgata
     6901 gctgcctccc cacgaaaatg agatttcaaa acggagttcg tcaatgtctg gaaactccgt
     6961 ttttcaggtg gcaactgctt ccaaccataa tcataggaag gaaaatactg aattttccct
     7021 tgttcattga taaaattctt agcataaaca tcataacggc cagcaaacac agaggtaaac
     7081 aattgtaatt tttcttgcgt cgtcaagcga ctactttgaa gttggaacat ctcctcgaac
     7141 tgagagcgtt ccataacaaa ctgactcttg tctttaagac gtttatagtg gaatgaaata
     7201 agatatgaac aaattgatta ggaaaatcaa attaatttat agaaatcttt tagcagtcat
     7261 ggcgtcctat tccagattca aaatgctata cacaagaaaa actcactatc cccattaaaa
     7321 gaagaaaaga catcaaggac ttctaccaca attccatcca aagacacaaa aacagccata
     7381 agagtcacct ccttgattcc tataggctga ttataacaag actggctgaa attgtacatg
     7441 aaaataaaat cctaatagta ctcattttgt atgtgactaa tattccgtct cgctccagaa
     7501 ggtacgaagt aaatagagtt taaacgagca gtctataaaa ttcaaaaaac gcatattatc
     7561 aagcgaccta aaactttggg ctctataata tttgtagtgg gtaaattccc tatggatatt
     7621 atggagccta ttttgggtag aaaaaaagtc ccataagatc tataatgaaa agcgacaaaa
     7681 caactcatta gaaagaatca tatggaacaa ttacatttta tcacaaaatt actagacatt
     7741 aaagacccta taaaattcct taccttgaaa cgactggtat gcctactaga attctcctta
     7801 gaaaacgtcg ttttaagtgc tatcattgct tgaaaatgat ggtcgctgaa acttctatcg
     7861 tcaagaaaaa tcaccaaatt cctcgtatta tcaaccaaaa gattgcgcaa aagttaattg
     7921 aaaagatttc tatgaccgat attgcccatc agctggccat ttcaacttca actgtcattc
     7981 gaaagctcaa tgacttccac tttgagtgta attttagaaa tctgcctaag attatgtctt
     8041 gggacgttga aacagtccgg ggagtgactg tttcaatcgg gagatggaga tgagctttat
     8101 tgcgcaagat tttgaaaagc tcgatatcat cactgttctt gagggtagaa cacaagctgt
     8161 catcc
//
LOCUS       DBEJAQ010000066         7999 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.066, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000066 DBEJAQ010000000
VERSION     DBEJAQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.066"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(1..304)
                     /locus_tag="ACL297_001882"
                     /pseudo
     CDS             complement(1..304)
                     /locus_tag="ACL297_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
     gene            473..1015
                     /locus_tag="ACL297_001883"
     CDS             473..1015
                     /locus_tag="ACL297_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGR0072086.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            1015..1644
                     /gene="nth"
                     /locus_tag="ACL297_001884"
     CDS             1015..1644
                     /gene="nth"
                     /locus_tag="ACL297_001884"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGR0072087.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            1855..2376
                     /gene="pyrR"
                     /locus_tag="ACL297_001885"
     CDS             1855..2376
                     /gene="pyrR"
                     /locus_tag="ACL297_001885"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGR0072088.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            2395..3318
                     /locus_tag="ACL297_001886"
     CDS             2395..3318
                     /locus_tag="ACL297_001886"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HGR0072089.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            3368..4447
                     /locus_tag="ACL297_001887"
     CDS             3368..4447
                     /locus_tag="ACL297_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HGR0072090.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            4759..7935
                     /gene="carB"
                     /locus_tag="ACL297_001888"
     CDS             4759..7935
                     /gene="carB"
                     /locus_tag="ACL297_001888"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGR0072091.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         2322 a         1617 c         1802 g         2258 t
ORIGIN      
        1 acaggtccat agagacttag tacgtgtttg acatactcgt aatggatttt atcatagttt
       61 cgccaagcag tgcgtgcttg attgtttagt tttaggctac ccagtccaat cagaggactg
      121 gtgggttggt aaaatttctc aagattaatt aagatactgt tgttcaactt ttctgttttt
      181 tgagctcttg catagtagtg tttagcagct cttgtagtcg aatatcatct gccgtaacct
      241 gtttacagtt ttggtagctt ttatttaact tggataggag ttgaatgagt ttcttgtctt
      301 ccatgtgagc atcctccttg atctgatgta ctctttccct atagtatagc actaaaaaat
      361 aaaactatca tagtaaatat gttaaaattt gaattttaat tacaaagttt ggtgtgctga
      421 tttgtagcct tttcatggta taatcaagag agtaaatctt tatggaggaa gtatgaaatt
      481 aaatattcaa gaaattcgta agcagtctga aggtttgaac tttgaacaaa cgttagattt
      541 agttgatgac ctgcgtgcac gtaatcaaga aattttagat gtaaaagata tccttgcagt
      601 tgggaaagta caatatgaag accgtatgta tttcttagat tatcaactat cttataccat
      661 tgttcttgct tcgagtcgca gtatggagcc agttgagtta gttgaatctt atccagtcac
      721 ggaagttttc atggaaggcg caactaacca gctagatcaa gaagttttag atgatgactt
      781 ggtcttgccc atcgaaaatg gggcgcttga ccttgctgag agtgtatcag acaatatcct
      841 gctaaacatt cctatcaagg tcttgacggc tgaagaagaa gctggtcaag gatttgtctc
      901 aggaaatgac tggcaaatca tgacagagga agaataccaa gctcaacaag cagtaaagaa
      961 agaagaaaac agtccttttg ctggcttaca aggactattt gacggagatg aataatggtc
     1021 ttgtcaaaaa aacgagcacg aaaggtgcta gaagaaatca ttgccctctt cccagatgcc
     1081 aagcctagtc ttgattttac caatcatttt gaactcctgg ttgcggtcat gttatcagcc
     1141 cagacgacag atgcagcggt aaataaggcc acaccaggtc tctttgttgc ctttccaaca
     1201 ccccaagcta tgtctgtagc gacagagagt gagattgctt cacatatttc tcgcttggga
     1261 ttgtatcgga ataaagctaa attccttaaa aaatgtgccc aacagttact agacgatttt
     1321 gatggtcaag tccctcagac acgtgaagaa ttggagagtt tggcaggtgt tggtcgcaag
     1381 acagccaatg ttgtcatgag tgtaggattt gggattccag cctttgcagt ggatactcat
     1441 gtggagcgta tttgcaaaca ccacgatatt gtcaaaaaat cagcgacgcc acttgaggtg
     1501 gaaaagcggg tcatggatat cttgccgcct gagcagtggt tagctgccca tcaggccatg
     1561 atttattttg gaagagccat ttgccatcca aaaaatccag agtgtgacca gtacccacaa
     1621 ttatatgatt ttagcaattt gtaagatgct acattgaaac tttttgcttg attttaagca
     1681 tgctttgtgc tatagtaaat gataactaaa tattccttta aacctgtccc gtgaggcagg
     1741 caaggagcgc gataaagtag aagggtgaag tctacactgt gtgcagtagg gctcgcttgg
     1801 ctataaattt caagtctcct tgtttaagga gacttttctt tttggaggtt tgtcatgaaa
     1861 acaaaagaag ttgtagacga attgaccgtc aaacgagcga ttacgcgtat tacttatgag
     1921 attatcgaac gcaacaaaga tttgaataag atcgtcttgg ctggtattaa aactcgtggt
     1981 gtctttattg cccaccgaat ccaagaacgt ttgaagcagt tagaaaatct ttcagttcct
     2041 gttgtggaat tggatactaa acctttccgt gatgatgtta aaagtggaga agatacttct
     2101 ttggtttctg tcgatgtgac agaccgcgaa gttatcttgg tggatgatgt gctctataca
     2161 ggtcgtacca tccgtgctgc tattgataat attgtcggtc atggtcgtcc tgcgcgtgtg
     2221 agtttagcag ttctagtcga tcgtggacat agagaattgc caatccgtcc agattacgtt
     2281 ggaaaaaata tcccaaccag tcgttctgaa gaaatcatcg tagagatgac agaacttgat
     2341 gaccaagaca gagttctgat tactgaagaa gcttagaaag ttaaaggagt agccatgtca
     2401 gaaaatcaac aagcattgaa ccatgtggtg tccatggaag acctcactgt cgatcaagta
     2461 atgaaattga tcaagcgagg aattgagttt aaaaatggag cccagcttcc ctatgaagac
     2521 catccgattg tttccaatct cttctttgag gattctacac ggacacataa gtcctttgaa
     2581 gtcgcagaga ttaaacttgg attggaacga cttgactttg atgtgaagac tagttcggtt
     2641 aataagggtg agacacttta tgacaccatt ttgactctgt ctgctttagg agtggatgtc
     2701 tgtgtgattc gccatccaga ggtcgactac tatagagaat tgattgctag tccaacgatt
     2761 acgacttcca tcatcaatgg tggagatggt tcgggccaac accctagcca gagcttgctt
     2821 gatttgatga ccatttatga ggaatttggc cactttgagg gtcttaaagt tgctattgca
     2881 ggtgacttgg atcactcacg cgttgccaaa tccaatatgc agattttgaa acgcttggga
     2941 gctgaactct ttttcgctgg acctgaggaa tggagaagtc aagagtttgc agactatgga
     3001 cagtttgtaa ccattgatga aatcattgat caggttgatg tcatgatgtt tctccgtgtg
     3061 caacacgaac gccatgatag tggagctgta ttttcaaaag aagactacca tgcccaacat
     3121 ggcttgactc aagaacgtta cgatcgtttg aaagaaacag caatcctcat gcacccagct
     3181 ccaatcaatc gtgatgtaga aatagcagac cacttggttg aagcaccaaa atcacggatt
     3241 gtccaacaaa tgaccaatgg tgtctttgtt cgaatggcaa tcttagaatc cgtactagcg
     3301 agtagaaacg ccaactaaaa cacaagacgt taaaagacgc cagtgagcgt ccacgagagg
     3361 tgaaaatatg acaaaaagaa ttctagtatt agaagatggc acagtttttg aaggcaaggc
     3421 cttcggagca gatattgatg taacaggcga aatcgtcttt aatacaggga tgaccggcta
     3481 ccaagaatcc attacagacc agtcttataa tggacaaatc ttgaccttta cttatccttt
     3541 ggtaggaaat tatggtatta atcgtgatga ttatgaatcc attattccaa cttgtaaggg
     3601 agtcgttgtt tttgaagaag cgcgtagagc tagcaactgg cgcaaccaaa tgaccttgga
     3661 tgaatttttg aaagccaaga aaattccagg gatttcagga attgatacgc gtgctcttac
     3721 caagattatc cgtaagcatg gtactatgcg tgcaaccttg acccatgttg gggacagtat
     3781 ggaccatgtg acggaccagc tccaagcaac agttttaccg acagacaata tcaaacaggt
     3841 ttctactaaa acgtcttatc cagctccagg agttggtttg agcgtggtgc tagtggactt
     3901 tggtctcaag cactcaatcc tacgtgaact ttctaaacgc aactgtaacg tgacagttgt
     3961 tccttattcg acaacggctg aagaaattct ccatctcaat cctgatggag ttatgttgtc
     4021 aaatggtcca ggtaacccag aagacgttcc acaagcactc gacatgattc gcggtgtaca
     4081 aggaaaaatt ccaatctttg gtatttgtat gggacaccaa ctctttgcaa tggcaaacgg
     4141 ggctaagacc tataagatga agtttggcca tcgtggattc aaccatgcgg tacgtgaaat
     4201 cgcaacagga cgagtagact tcaccagcca gaaccatggt tatgcagtca gccgcgagga
     4261 tttgccagag cacttgatta ttacccacga agaaatcaat gacaagtcag tggaaggtgt
     4321 gcgccacaga taccaacctg ctttctctgt tcaataccac ccagatgcag ctcctgggcc
     4381 acacgacgct agctacctat ttgacgagtt tatcgagatg atggaagttt ttaaacaatc
     4441 aaactaagaa cgagtaaaag ctcgtcggag aatttatgta actgaacacg gacggaaagc
     4501 tcagaattta gagaaaccaa cttggattgt caatccttcc ttgatttctt aaaattcaat
     4561 cgctttctat tcgtcctttg tatcttattt aatgtcgccc tgagaggcga cgaatagaaa
     4621 ggagaagata cattgttcgc ggaagtgaac acgccctaag aactgtgtga aaaagataaa
     4681 catcgtcttg acgctgaagg cgtctgcgcc gagtttccta ttttcactgt gttctttacg
     4741 ggctttgtat cataaactat gcctaaacgt actgatattc aaaaaattat ggtgattggt
     4801 tctggtccga ttattattgg tcaggctgct gagtttgact acgctgggac ccaggcttgc
     4861 ttgtcgttga aagaggaagg ttatgaggtt gttttggtta actcaaatcc tgcaaccatc
     4921 atgacggaca aggaaattgc tgacaaggtt tacattgaac cgattacact tgagtttgtg
     4981 acacgtattc ttcgtaagga acgtccagat gccttgctac caacactcgg tggtcagaca
     5041 ggtctcaaca tggccatgga attatctaaa aatggtatcc tagacgagct tggcgttgaa
     5101 cttctgggta ccaaattatc tgccattgac caagcggagg accgtgacct ctttaaacaa
     5161 ttgatggaag agcttgagca acccattcca gaatctgaaa ttgttaacac agtagaagaa
     5221 gcagttgcct ttgcagcgac aattggctac ccagtcatcg ttcgtccagc ctttaccctt
     5281 ggtggtactg gtggtggtat gtgtgccaac gaaaaagaat tgcgtgaaat cactgaaaat
     5341 ggtttgaaat tgtcacctgt tacccaatgt ttgattgagc gttcgattgc cggttttaag
     5401 gaaattgaat acgaagtgat gcgtgactca gctgacaatg ccttggttgt ttgtaacatg
     5461 gaaaactttg acccagttgg gattcacaca ggggattcca ttgtatttgc ccctgcgcaa
     5521 accatgtcag actatgaaaa ccaaatgctt cgtgatgcga gcttgagcat tattcgcgcc
     5581 ctcaagattg aaggtggatg taatgttcag ctagctcttg atcctaacag cttcaagtat
     5641 tatgttatcg aagtaaaccc tcgtgtatca cgttcgtcag cccttgcttc taaagcgaca
     5701 ggatatccga ttgctaagtt ggctgccaag attgcagtag gtttgacctt ggatgaggtt
     5761 ataaacccag ttacaggttc aacctatgcc atgtttgaac cagcccttga ctacgtagtt
     5821 gctaagattc cacgtttccc atttgacaag tttgaaaagg gtgaacgccg tctcggtaca
     5881 caaatgaagg cgactggaga agtcatggca atcggtcgta acatcgagga atctcttctc
     5941 aaggcctgcc gctcccttga aattggggtg caccacaatg aaatacctga acttgcagca
     6001 gtttctgatg atgccttgat tgaaaaggtt gtcaaagccc aagatgaccg tctcttctac
     6061 gtatcagaag ccattcgtcg tggctacaca cctgaagaaa ttgcagagct tacaaaaatt
     6121 gatatcttct atcttgataa actcttgcat atctttgaaa ttgagcaaga attgggtgct
     6181 catccacaag atctagaagt tttgaaaaca gccaaactta atggtttctc agaccgtaag
     6241 attgctgaac tctggggaac gactgatgat caagttcgcc aacttcgctt ggaaaacaag
     6301 attgttccag tttataagat ggtagatact tgtgcggcag agttcgactc tgaaacgcca
     6361 tatttctatt caacctatgg ttgggaaaat gaatctatca gatctgataa agaatctgta
     6421 cttgtcctag gttcaggtcc aatccgtatc ggtcaagggg ttgagtttga ctacgcaacc
     6481 gttcactctg ttaaggctat ccaggcagct ggttatgaag ctatcatcat gaactcaaac
     6541 ccagagaccg tttcaacaga cttctctgta tcagataagc tttactttga gccattgaca
     6601 ttcgaagatg ttatgaacgt cattgacttg gaacaaccaa agggagttat cgttcagttt
     6661 ggtggtcaaa cagccatcaa ccttgcggag ccattggcaa aagcaggtgt gaccatcctt
     6721 ggtacacagg ttgctgacct agaccgtgcc gaagaccgtg acctcttcga gcaagctctc
     6781 aaagaattgg atattccaca gccaccagga caaacggcta ccaatgaaga agaagcagct
     6841 cttgcagctc gtaagattgg cttcccagtt cttgtccgcc catcttatgt acttggtgga
     6901 cgtgccatgg aaatcgttga aaacgaagaa gaccttcgtt cttacatgcg aactgctgtt
     6961 aaggctagtc cagatcaccc agttcttgtc gactcttata tcgttgggca agagtgcgaa
     7021 gttgatgcca tttcagatgg taaagatgtg ctcattcctg gtatcatgga gcatatcgaa
     7081 cgtgctggtg tccactcagg tgactcaatg gctgtttacc caccacaaac cttgtcgcaa
     7141 aaggttcagg agaccatcgc agactacacc aaacgcttag caatcggtct tcactgcctt
     7201 ggaatgatga acatccagtt tgttatcaag gatgaaaaag tctacgttat tgaggtcaat
     7261 ccacgtgcca gccgtactgt tccattccta tctaaggtaa ccaatattcc tatggctcaa
     7321 gtagcgacca agctcattct tggtcaaagt ttatcagaac ttggctacca aaatggactt
     7381 taccctgaaa gcacccgtgt tcatatcaag gcacctgtct tctcctttac caaactagct
     7441 aaggtagaca gcttgctcgg tcctgaaatg aagtcaacag gtgaagttat gggttctgat
     7501 gctactttgg aaaaagctct ctataaagcc tttgaagctt cttatctaca cttgccaact
     7561 tttggtaatg tcgtctttac catcgctgat gatgccaaag aagaagcctt gaacttggct
     7621 cgtcgtttcc aaaacattgg ttatggaatc cttgcgacag aagggacagc agccttcttt
     7681 gctagtcatg gcttgcaagc ccaacctgtt ggtaagattg gtgatgacga taaggatatc
     7741 ccaagttttg tacgcaaagg aagaattcaa gctatcatta acacagtagg aacaaaacga
     7801 actgctgacg aagatggtga gcaaattcgt cgttcagcca ttgaacacgg ggtgcccctc
     7861 ttcacagcgc tagatacagc taatgccatg cttaaggtac ttgaaagtcg tagctttgtt
     7921 acagaagcga tttaaataga aaaataagat atagcagact gaatttagaa cagtacacag
     7981 gaatactaaa atatttcta
//
LOCUS       DBEJAQ010000067         7582 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.067, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000067 DBEJAQ010000000
VERSION     DBEJAQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.067"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..103
                     /locus_tag="ACL297_001889"
     CDS             <1..103
                     /locus_tag="ACL297_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGR0072092.1"
                     /translation="NYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            99..773
                     /locus_tag="ACL297_001890"
     CDS             99..773
                     /locus_tag="ACL297_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HGR0072093.1"
                     /translation="MDSNFAGRLNILRAGVLDANDGIISIAGVVIGVASATTNIWIIF
                     LSGFTAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQN
                     GEFKTSAQLLTNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPML
                     SVTIFPSDYRIPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLL
                     EQLFSI"
     gene            complement(815..2803)
                     /locus_tag="ACL297_001891"
     CDS             complement(815..2803)
                     /locus_tag="ACL297_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0072094.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFTKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2805..3563)
                     /locus_tag="ACL297_001892"
     CDS             complement(2805..3563)
                     /locus_tag="ACL297_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0072095.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3991..4182
                     /locus_tag="ACL297_001893"
     CDS             3991..4182
                     /locus_tag="ACL297_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072096.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(4536..4808)
                     /locus_tag="ACL297_001894"
     CDS             complement(4536..4808)
                     /locus_tag="ACL297_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGR0072097.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(4867..5316)
                     /locus_tag="ACL297_001895"
     CDS             complement(4867..5316)
                     /locus_tag="ACL297_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HGR0072098.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            complement(5303..7432)
                     /locus_tag="ACL297_001896"
     CDS             complement(5303..7432)
                     /locus_tag="ACL297_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGR0072099.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2261 a         1597 c         1482 g         2242 t
ORIGIN      
        1 caactatcct aaaaaatgct tggactacaa gtcacccaga gaagacttct ggatggctaa
       61 cttgaacttg aaatttagcg tcagaaacaa atcacgaaat tgattcaaat tttgcaggtc
      121 gtttaaatat cctgcgtgcg ggtgttcttg atgctaacga tggaattatt tccattgctg
      181 gtgtggttat cggagttgcc agtgccacga ccaatatctg gattatcttt ttatcaggtt
      241 ttacggctat cttagctggt gccttttcaa tggctggtgg agaatatgta tccgtttcaa
      301 ctccaaaaga taccgaggaa gctgccgttt cgcgagaaaa actcttgcta gaccaagata
      361 gggaactagc caaaaaatcc ctctatgctg cttatatcca aaatggagaa ttcaaaactt
      421 ctgcccaact cttgaccaat aagatctttc ttaaaaatcc actcaaggct ctggtagagg
      481 aaaaatatgg gattgagtat gaagaattta ccaatccttg gcacgctgcc atttctagct
      541 tcgttgcctt tttccttaga agtttgcctc caatgctgtc agtgaccatt tttccaagtg
      601 attaccgcat ccctgctacc gtccttattg tcggtgtggc ccttcttctc actggttaca
      661 caagtgctag acttggaaaa gccccaacca aaacagctat gattcggaac cttgctattg
      721 gtctcttgac catgggagtt accttcctgc tcgaacaact tttcagcatt tagaatacaa
      781 gaaatacctc gattttgaag tcgaggtatc ttttttacat ttgcacaatc ttgcgataac
      841 ttcttgaagt aatcatgaaa atcagcacat aggcgatgag gaagatagcg cagatagaca
      901 aggtcacaat caacatcata gtcgtatcca gtacaccaat cacttttaaa atcaggctaa
      961 gcatatggta ggcaaaggcg agatgtatga aggcaaagag caaaggaagg aagaaaacag
     1021 ttaaaacctg tttgttgatg gtttgcttga tttgcttttg gtccaaaccg actttctgca
     1081 agataataaa gcgttcacgg tcttcgtagc cttcagaaat ttgtttgtag tagatgacca
     1141 gaacagttcc gaccataaag ataatggata ggaaaatacc gataaagaag acaccaccaa
     1201 agagggcact catctgagaa ctagcatctg ctagattgct accataaaca tagctacctt
     1261 ctgtgtctaa ttgagcatta aattgattga ggtagttttc atactcctca gcgaccttga
     1321 gttgttcttc ttcgctggca tctacattca taccaccgta aaactgatta tagatagccg
     1381 aatctgggaa ttgatccaaa aaggcttgta aatcaggaac aacaaggtaa ttgtaatcag
     1441 tagtcaagat attaaactta tttggaacat ggttgacaat aaaatcttta gtaaattctt
     1501 ctttgacaga aaattgatga tcatttagag ttagagcttt ctgtcctttc agtccgtcat
     1561 ttttggcaaa gagaccgacc tcatttcctg atagagacag tttttgacca gtcatatttt
     1621 cataatcttt ttggtcaaat accatgaaaa ctgtttttgg ttggacacgg ttttgtcctt
     1681 tttcaaaaat agttaacttg gttccttctt gattcgcaac agcaaagtaa gtgtaacgaa
     1741 atacttcttt ttctttaatc ttataacctt tgtcacttgc aaactggctc aagagtttgt
     1801 ccaagtcttc tttttcaaca ttttgccctg aaaccccaaa atcatgagga tttagaactt
     1861 ttttaaagct ttctgaggaa ttgaaaatgc ttgtcgctgc tgacatggtt accaaaacca
     1921 ttgttgacaa gatagcgatg gtggctagtc caactgcatt tttcttcata cggaaaatca
     1981 agttagaaac agatatgagg ttatttggtt ggtaatagta tttcttattt ttctttaaga
     2041 tttggaggaa aacggtaatc cctgcattaa acaagagata agtcccaaag ataaccagta
     2101 aaacagctat gaagaaggtt gttaaggctg taagaggatc ttttaccgta agggcaagat
     2161 aatagccaat tcctaaactt atggaaccaa gaatggtttg gagaggaagg aagcgacctt
     2221 ttttctctcc actagctttc tcacgagaga gctggagggc attcatacgg gcgattcgaa
     2281 gggcattcag gaacatgagg cctaggaaaa tcaaaccgaa gacaacaagc actgtaatga
     2341 caactttcgt ctggaaggta gcaaccagct caaccttcaa tttcattagt ttgagcagga
     2401 aagcgaaaat taacttgtca aacaaggctc caataccgat acccgctcca acagttagaa
     2461 tcccaaatac cactaactcc ttaaaggtca tactgataag atgacgcttc tccaagccca
     2521 acatgccata aattcctagt tccttggaac ggttcttcat gacaaaacta ttggcataga
     2581 gaacgataat ggctgacgca agggtgacga caaacatacc aaatccaagt gtagcctgaa
     2641 tggttgttcc tccacggatt tccgcaatct taggattgaa ggttagagag taaaagagat
     2701 aggtgacagt gactgccaag agaacagcca gcgcaaaagg atagtagagt ttgcggtttt
     2761 taatcaagtt cgataccgct aacttattgg ttaatcgaaa catactaatt cacctcgctt
     2821 gccatgacag tcaaggtatc agagatttct tggaacatct gacgttctgt cttctctcca
     2881 cggtagattt gattgtaaag aatgccgtct ttgataaaga gtacacgttt agctctgcta
     2941 gctgctgctg ttgagtgggt taccatgaga atggtttggc cgcgctcatt gatttcatca
     3001 aagacatcaa gtaaggctgc agatgacttg gagtcaagag ctcctgtcgg ttcgtccgca
     3061 aggagaattt caggttctgt gatgatggcg cgggctactg ctacacgctg tttctgacca
     3121 ccagaaatct cgtaagggta cttctcttgt aattggttta ttcccagatt ctcagctgtc
     3181 accaccaatt tcttcatcat ctctgtaata ggtcttcttg acaagacaag cggaagcaag
     3241 atattgtcct ttacagatag agtatctagc aagttaaagt cttggaagac aaagcccaac
     3301 ttttcacgac ggaagctaga ggcctgtgaa tttttaatgg ttgcggtgtc agttccattc
     3361 aagtaaacct gaccacgact tggtttatcc agcatagcta gaatattgag aagagttgat
     3421 ttaccagaac cagactcacc catgatggca acgtagtcac ccttttcgac ggtaaagtga
     3481 atatccttga gggcttctac ttggttgccc tgaaaacgtg ttttataaat tttttgaacg
     3541 tgttttacat ctaaaagtgt catgagaatc tcctttctta atatcccatc aaatgaaatg
     3601 tagcttgcat catagtgcat cccagctgaa cgcgctcaat ttctttgtga cttggttttc
     3661 tagttttctt atgtaagatt tgtttcatga tgttactcct ttgttcttta tgagtctagt
     3721 ttacatcaaa aaaagaccgc ttgccataac ctaaccttac aaaagagact ttaatcttac
     3781 atttttgtaa gattgggtga aatataagca aaattactta gaaaatgatt tgatttccta
     3841 tttttaaagt tgtaaaatat aagtgtgaaa aggcttactt tgaaaaggag gaagcgggaa
     3901 ctaacatttt agtaaggtta cctagataac ataccattca atttaacact tgcaagtcgt
     3961 tcaacctata aggaaagaag gtgcgcttta atgaaacaca aagaacatat actcatcgga
     4021 cttctctatc tgctctctcc attcatcggt cagctcttag ttgaacacac acactttatc
     4081 ggtacagaat ttaccggcac tgcttatgtt atttgctggt tatcagttgt tatcagcatc
     4141 caccattttt ccaaaaatgt attgtctcag cagcaaaaat agattaaaaa actgagttta
     4201 aattactgct tttataataa aatgaaataa gaacaggaca aatcgatcag gatagtcaaa
     4261 tcgatttcta acaatgtttt agaagtagag gtgtcctatt ctagttccaa tccactatag
     4321 tttgtggatg aagctggcga aatattatta atactattca aaaatcaaag tgcaaactat
     4381 gtcaacttca cattgccgac taaattacag cctgtggctg gcttcctagt ttgcactttg
     4441 atttttattg agtataaaag taaaaaagct ttaaatcaag cagtttagac tattgattta
     4501 aagctttttg cttattccaa atcttctgta gaacctcacc aaaaccagcc ttcattgtcg
     4561 tcaatggctt gggcttcttt gcgtttacgt gggcctgttc cgtacatttc tgcttcgatt
     4621 tcttccttgg taggcatgat agaagctagg atgatataga taatcacgcc aagtccaaag
     4681 tttgcaactg taaaaatagc aaacaagaaa cgaactaggg taacatcaaa attccacttg
     4741 tctgacagac ctgccagaac tcctgaaatc atgcgattgc gtctcatttt ataaaattta
     4801 ctgttcatga tatttcctct ttctgctagg ttagacatag tatatcacgg ggaggctcgg
     4861 ctttcatcag tcctcaggct gatttatcag aagcaattta cctcgacaaa gtccacagcg
     4921 atagcgtttg gtatcaatcc tacgcttgcg ctgatacctt tgctggcagg attgacaacg
     4981 ataaagcttg agtggtttag agttactatt gggcaaggat ggtacaaacc gtaatccatc
     5041 cactgctttc aaaagttcct taaaatcccg atccttgtgt tgatagccct tcccttggaa
     5101 ataaaggtga taatgacaga gttcatgtcg gacaattttc ctaaaaacct ccatacccag
     5161 tttctgataa accttgggat taaaatccag atgcccatct ttggggaaaa atcgcccacc
     5221 tgtcgaacgt agacgcctat tccactggac atgatggata aaagctctcc cgaagtcttc
     5281 tagtgaaacc tgcttgacat aatcagtcag tttcatttgg agcgaggagc gacagattaa
     5341 ctttttcacg ttcagtatca attttcttga cccaaacggt caccaaatct ccaactgcca
     5401 ccacttgact agggtgtttg ataaacttgc gactcatatg agaaatatga atcaagccgt
     5461 cctcgtgaat cccgatatca acgaaggcac cgaagtcaac aacgttacgc acaacacctt
     5521 ctagcttctg acctaccact aggtctttga tatctaggac atcttggcga agcacaggtg
     5581 cgtcaaagga atcacggaaa tctcgacctg gtttgagaag atctgcaatg atatctttaa
     5641 gggtttctgg accgaggtct agctcttgag ccatttcctt gactgaaagc gacttgagtt
     5701 tgctttgggc ctcttcattc agatccttaa tgtccaagca tttgaagagt tccttaacgg
     5761 cagcgtaatt ttctgggtga actcctgtat tatcaaggat attgctactt tcagggatac
     5821 ggaggaaacc agcagcctgt tcaaaagcct tggcccccaa acgaggaact ttcttgattt
     5881 gggagcgtga agtgattttt ccctcttcct cacggtattt gacgatattt tcagaaatgg
     5941 ttttattgag tccagctacg tgtgaaagaa gagctgggct agctgtattg acattgacac
     6001 caacttggtt aaccactgta tcgacaacaa agtccagact ctcagatagt ttcttctgac
     6061 tgacatcgtg ttggtattga ccgacaccaa ttgacttagg atcgattttg accaattccg
     6121 caagaggatc ttgcaaacga cgggcgatag aaatggcaga gcgtttttca acggtcaagt
     6181 ctggaaactc ctgacgagca agttcgctgg cagaatagac agaagcacca ctttcattaa
     6241 cgataacata gctgacttca gggaaatctt tcagaacttc cgctacaaaa gcttcacttt
     6301 cacgactggc cgttccattt ccaatggcaa taatctctac accgtattga ccaattaaat
     6361 ctgctaaatc tttcttggct tcttcgattt gacgagctga tgctggttta acaggataaa
     6421 taacctgagt tgtcagcatt tttcctgttg catccacgac agctagcttg gcacctgtac
     6481 gaaaggctgg gtcaaaccca agaaccacgc gccctttcag tggagcaacc aagaggagat
     6541 tgcgcagatt gtcagaaaaa agttggatag ctccctcttc agctttctca gttaattctg
     6601 tccgaatacg acgctcaata gcaggcaaga cctttttctt aacggattgc tgaacaactt
     6661 catcaatata agcatttttc accttgaaac gagtagcaaa gaaggcaaga atacggtccg
     6721 tcgcatgttc aaaaccgatc ttcaagacac caagtttctc cccacgattg agagccaagg
     6781 tacgatagcc ttgcatagtt ccaactgtct ctgaaaaatc ataataaatc tgaaaaacct
     6841 gcttttcatc aagactttca tccttggctt gagaagtgag tttagagtgt ctcagcactt
     6901 cctgataagt catagaacgc aaggtcacat cttccgataa ggcttcgacc aaaatatcaa
     6961 ctgcaccggt caaggcttcc ttgccagtcg caaatccttc acagacgaac ttttcagctt
     7021 ctttctctaa gtcaactata ttctgcaaaa tcaaacgagc aagaggaaag agtccagctt
     7081 cacgggcaat ggttgccttg gtacgacgct tttccttata aggaagatag agttcttcaa
     7141 cgtctgctaa tttttcggca actaagatag cttcttccaa ttccttggtc aacttacctt
     7201 gttcttgaat cttagctaag acagcttcct tacggtcatt gagatttgtc agacttttat
     7261 ccaaatcaat aatagcctta atcgccacct catccagact accagtcatg tccttgcgat
     7321 aacgcgcgat aaagggaata gtcgcccctt cagctgtcaa acttagaacg gtatcaattt
     7381 gctttaacgt cactcccaaa tcctgagaga ttttttcata ttttttatcc ataaatctat
     7441 tataccacaa gctggaaggc tttctccaat ccttttcata ctcaataaaa atcaaaaagc
     7501 aaactaggaa gctagccgta ggctgctcaa aatactgttt tgaggttgtg gataagactg
     7561 acgaagtcag ctcaaaacac tg
//
LOCUS       DBEJAQ010000068         7235 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.068, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000068 DBEJAQ010000000
VERSION     DBEJAQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.068"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            226..6660
                     /locus_tag="ACL297_001897"
     CDS             226..6660
                     /locus_tag="ACL297_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGR0072100.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         2850 a          979 c         1386 g         2020 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tagagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 ttagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcaaaggata ctgaagaaaa gaaaacaaca gttattgagg aaaaagaagt tgttagtaaa
      481 aatcctgtga tagacactaa aactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagataatgc acactcgtct gcaaataaag gcacagaaaa tccgaaaaaa
      601 gaagatagac ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 acatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgagg atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccagatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgca gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctattcgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcag gctgatgaaa aggggatgat ttcctattat
     4201 ctagtagatc ctgatcaaga ttcatctaaa tatcaaaagc ttggcgagat tgcagaatct
     4261 aaatttaaaa atttaggaaa tggaaaagag ggtagtctaa aaaaagatac aactggggta
     4321 gaacatcatc atcaagaaaa tgaagagtct attaaagaaa aatctagttt tactattgat
     4381 agaaatattt caacaattag agactttgaa aataaagact taaagaaact cattaaaaag
     4441 aaatttagag aagttgatga ttttacaagt gaaactggta agagaataga ggaatacgat
     4501 tataaatacg atgataaggg aaatattatt gcttatgacg atggtagtgc cttacaatat
     4561 gaaactgaaa aacttgacga aataaaatca aaaatttatg gcgttctaag cccatctaaa
     4621 gatggacact ttgaaattct tggaaagata agcaatgttt ctaaaaatgc caaggtatat
     4681 tatggaaata gctataaatc gatagaaatc aaagcgacta agtatgactc acattcaaaa
     4741 acgatgacat ttgatttata cgctaatatt aatgatattg tggatggatt agcttttgct
     4801 ggagatatgc ggttttttgt taaagatgat aatcgaataa aagctgaaac taaaattaga
     4861 atgcctgaaa aaaataagga aactaaagca gaatatccct atgtatcaag ttatgggaat
     4921 gtaatcgaat taggagaagg agatctttca aagaacaaac cagacaattt aactaaaatg
     4981 gaatctggta aaatctattc tgattcagaa aaacaacaat atctgttaaa ggataacatc
     5041 attctaagaa aaggctatgc actaaaagtg actacctata atcctggaaa aacggatatg
     5101 ttagaaggaa atggagtcta tagcaaggaa gatatagcaa aaatacaaaa ggccaatcct
     5161 aatctaagag tcctttcaga aacaacaatt tatgctgata gtagaaatgt tgaagatgga
     5221 agaagtaccc aagctgtatt aatgtcggct ttggacggct ttaacattat aaggtatcaa
     5281 gtgtttacat ttaaaatgaa cgataaaggg gaagctatcg ataaagacgg aaatcttgtg
     5341 acagattctt ctaaacttgt attatttggt aaggatgata aagaatacac tggagaggat
     5401 aagtccaatg tagaagctat aaaagaagat ggctccatgt tatttattga taccaaacca
     5461 gtaaaccttt caatggataa gaactacttt aatccatcta aatctaataa aatttatgta
     5521 cgaaatccag aattttattt aagaggtaag atttctgata agggtggttt taactgggaa
     5581 ttgagagtta atgaatcggt tgtagataat tatttaatct acggagattt acacattgat
     5641 aacactagag attttaatat taagctgaat gttaaagacg gtgacatcat ggactgggga
     5701 atgaaagact ataaagcaaa cggatttcca gataaggtaa cagatatgga tggaaatgtt
     5761 tatcttcaaa ctggctatag cgatttgaat gctaaagcag ttggagtcca ctatcagttt
     5821 ttatatgata atgttaaacc cgaagtaaac attgatccta agggaaatac tagtatcgaa
     5881 tatgctgatg gaaaatctgt agtctttaac atcaatgata aaagaaataa tggattcgat
     5941 ggtgagattc aagaacaaca tatttatgta aatggaaaag aatatacatc atttgatgat
     6001 attaaacaaa taacagacaa gacactaaac attaagattg ttgtaaaaga ttttgcaaga
     6061 aatacaaccg taaaagaatt cattttaaat aaagatacgg gagaggtaag tgaattaaaa
     6121 cctcataggg taactgtgac cattcaaaat ggaaaagaaa tgagttcaac gatagtgtcg
     6181 gaagaagatt ttattttacc tgtttataag ggtgaattag aaaaaggata ccaatttgat
     6241 ggttgggaaa tttctggttt cgaaggtaaa aaagacgctg gctatgttat taatctatca
     6301 aaagatacct ttataaaacc tgtattcaag aaaatagagg agaaaaagga ggaagaaaat
     6361 aaacctactt ttgatgtatc gaaaaagaaa gataacccac aagtaaacca tagtcaatta
     6421 aatgaaagtc acagaaaaga ggatttacaa agagaagatc attcacaaaa atctgattca
     6481 actaaggatg ttacagctac agttcttgat aaaaacaata tcagtagtaa atcaactact
     6541 aacaatccta ataagttgcc aaaaactgga acagcaagcg gagcccagac actattagct
     6601 gccggaataa tgtttatagt aggaattttt cttggattga agaaaaaaaa tcaagattaa
     6661 gacaaaagct atagaaaaaa atggtttatg tactgagatt agatagtgag gtgatgacat
     6721 agttttgtga aaacggccat ttataactca attatttagt ttacttcact tcactattga
     6781 tactatttgg agttattaat ggacttagtt tagataacta atgaattgat tgaaagggtt
     6841 agtattgaca atattggtca tattgattag aaaatagagt ctatcaaaat ttaaaggcta
     6901 atagaggtga tgagacaatt tcaaggaatt aagtagcgtt tggttgctta attccttttt
     6961 atttggaagg aagtttttcg taaccgccca ataacgaagt ctattggacg gttacgatat
     7021 gatcactgac ttcgtcagtc ttatctacaa cctcaaaact gtgttttgag caacctgcgg
     7081 ctagcttcct agtttactct ttgattttca ttgagtatta gaacagaaaa aatgcttgga
     7141 gtatttgttt gtgtgtttat ttttatataa caaactataa acaaaacaaa aatataaaaa
     7201 aagagacaaa aaagaacaaa aagtaattga caacg
//
LOCUS       DBEJAQ010000069         7198 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.069, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000069 DBEJAQ010000000
VERSION     DBEJAQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.069"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(212..1174)
                     /gene="nrdF"
                     /locus_tag="ACL297_001898"
     CDS             complement(212..1174)
                     /gene="nrdF"
                     /locus_tag="ACL297_001898"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGR0072101.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1362..3521)
                     /gene="nrdE"
                     /locus_tag="ACL297_001899"
     CDS             complement(1362..3521)
                     /gene="nrdE"
                     /locus_tag="ACL297_001899"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGR0072102.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(3602..3820)
                     /gene="nrdH"
                     /locus_tag="ACL297_001900"
     CDS             complement(3602..3820)
                     /gene="nrdH"
                     /locus_tag="ACL297_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGR0072103.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            4171..4434
                     /locus_tag="ACL297_001901"
     CDS             4171..4434
                     /locus_tag="ACL297_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HGR0072104.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            4440..6173
                     /gene="ptsP"
                     /locus_tag="ACL297_001902"
     CDS             4440..6173
                     /gene="ptsP"
                     /locus_tag="ACL297_001902"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGR0072105.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            6428..>7198
                     /gene="phtA"
                     /locus_tag="ACL297_001903"
     CDS             6428..>7198
                     /gene="phtA"
                     /locus_tag="ACL297_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HGR0072106.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNW"
BASE COUNT         2215 a         1321 c         1551 g         2111 t
ORIGIN      
        1 aaaaacaaaa atatcgaagt ttgttttcaa aactttcgat atttttgttg ggttattatt
       61 ttgttgtttc tagtttactt tttgatggtt aagagtggtg gagaattata ctcaatgaaa
      121 atcaaagagc aaactaggaa gctagccgca ggctgtactt gagtacggca aggcgaagct
      181 gacgtggttt gaatttgatt ttcgaagagt attagtccaa accgtagttg tagtcatcat
      241 cttgcatggc ttcaacttcg ccaagaaggt aaccatttcc gacttgagag aagaagtcat
      301 ggttggaagt tcctgttgaa ataccgttca taacgattgg gttgacatct tcagctgaat
      361 ctgggaaaag tggatcttgt cccatgttca tgagagcttt attggcattg tagcgaagga
      421 aggttttaac ctcttcagtc caaccaacac cgtcataaag actctctgtg tagccttctt
      481 cattttcata aagagtatag agtaggtcgt acatccattc tttgagtttt tcttgctctt
      541 cttcaggtaa ttcattgaaa ccaagttgga atttgtaacc aatgtaggtt ccgtgaacag
      601 actcgtcacg aataatcaat ttaatgattt ctgcaacgtt ggcaagtttg ttgttaccga
      661 gatagtagag gggagtgaag aaaccagagt agaagaggaa ggtttcgagg aagacgctgg
      721 caactttctt ttcaagtggg ctgccgttta ggtagatttc gttgacaatc tcagccttct
      781 tttgtaggta aggattggta ttggtccatt cgaaaatttc ttcaatctca gccttagtat
      841 tcaaggtaga aaagattgat gagtaagatt tagcgtggac agattccata aattggatgt
      901 tattgaaaac agcttcctca tgtggtgtac ggatgtctgc gcgaagggct tgaaccccag
      961 tttcagattg catagtgtca agaagggtta aaccaccaaa aacttttccg accaagtctt
     1021 tctctttgtt agatagcttt ctccagtcat ccaagtcgtt tgataaggga atacgtgtat
     1081 cgagccaaaa ttgctccgtc agtttttccc aagttgattt gtcgatgaca tcttcgatgg
     1141 cattccagtt aatggctttg tagtaagttt ccatttaaaa tctctttctg tgtttagtat
     1201 tgcgaactca caattatttc tactttacca taattctata ggagtatcgc acaaaaagtc
     1261 ggaagcccga cttttaaaat gttacataaa ttatgttatg acatagtaga tttgatttta
     1321 tcagtgctgc ttagggaaaa atagtgtttc tatgctagaa actaaatcac acagctttca
     1381 cattggttgg cgccgacttc tccaccgtca tctgtaaagg tacggacgta gtagatagac
     1441 ttgattccct tgttaaaggc atagttacga aggatggaca agtcacgtgt cgtttgttta
     1501 ttttccctct tccattcgta aaggcctttt ggaatgtcac tacgcatgaa gagggtgagt
     1561 gaaagtcctt gatccacgtg ttcagtcgca gcagcgtaaa catcgatgac tttacgcata
     1621 tccatatcgt aggcagaagt gtagtaagga atggtttctg tagacaagcc agcagcaggg
     1681 tagtagattt taccaatttt cttttcctga cgttcttcaa tacgttgcgt aatcgggtgg
     1741 atagaagcag aaacatcgtt gatgtagctg atagaaccat ttggcgctac agcaaggcga
     1801 ttttggtggt aaagaccatc ctcttgaacc ttgtcgcgaa gttcagtcca atcagcaaca
     1861 ccagggataa aaacattttt gaagagttct ttaacacggt ctgatgttgg aacaaattca
     1921 ccagttacat acttgtcaaa gtaacttccg ttagcatagt ctgatttttc aaagttgtgg
     1981 aaggtaatac cacgttcacg cgcaatattg tttgactcta ccaaggtcca gtagttcata
     2041 agcataaagt agatgcttgt aaattcaaca gactcaggtg aaccatattc aatgagttgt
     2101 tgggcaaggt agctgtgcag tcccatggca ccgagaccaa aggtgtgggc ttggctattt
     2161 ccatggtcaa tcgttggtac agctacgata tgtgaactat ctgtaacgaa agtaagggca
     2221 cgaaccatag cacggataga acgaccaaaa tcaggtgaag tcatcatgtt aaccacgttg
     2281 gttgaaccca ggttacatga aacatctgtt cccatttgaa ggaattcttg agcatcgttg
     2341 atcaagcttg gttcttgaac ttgaagaatc tcagaacaca agttactcat gataatcttt
     2401 ccatcgacag gatttgcacg gttagccgta tctatgttga ctacataagg atagccagac
     2461 tcttgttgca atttagagat ttcagtttcc aaatcccgcg ccttgatttt tgtcttgcga
     2521 atatttggat ttgcgaccaa ttcatcgtat ttttcagtaa tgtcgatgta attgaatggc
     2581 acaccgtatt ctttttctac agagtaaggg ctgaagaggt acatttcttc atttttacga
     2641 gccaattcgt agaatttatc aggtactaca acaccaagtg atagagtctt gacacgtact
     2701 ttttcatcag cgttttcttt cttagttgaa aggaaagcga tgatatctgg gtgaaagacg
     2761 ttgaggtaga caacaccggc accttgacgt tgccccaatt ggttggagta agagaagctg
     2821 tcttcaaaaa gcttcataac aggaacgaca cctgaagcag ctccttcata gcctttgata
     2881 ggtgcaccag cttcacgaag gttgctgagg gtaattccca caccaccacc aatacgtgaa
     2941 agttgaagag ctgagttgat agaacgtccg atagagttca tatcatccgt cacttggatt
     3001 aggaaacaag ataccaactc cccacgacga gcacgtccag cattcaagaa ggaaggagta
     3061 gcaggttggt agcgttggtg gatgatttca ttggcaatat cgattgcaac agcttcattc
     3121 ccatcagcga aataaagggc gttgaagaag acacggtctt ccatattttc aagatagtat
     3181 tcaccgtcat tagtctttaa ggcatattga ttgtaaaatt tataagctgc catgaatgac
     3241 ttgaattgga agttttggtc tttgataaat tgagccaatt cttccaagaa ctctggacgg
     3301 tatttcttga taaaggctgt ttcgatgtag ttgtgttcaa tgaggtaatt gattttgtct
     3361 ttgattgaat caaaaaccat agtgtttgga actacatttt ctttaaagaa agcatccaag
     3421 gcttccttat ctttatgaag catgatttgt ccattaacag gacggttaat ttcgttatta
     3481 agacggaagt aagtcacgtc ttcaagatgt tttaatccca taaaatttcc cttatctaat
     3541 tacaaaagaa aggcttctaa gttagcccta aaagcagttt cttctggatg atgtactaag
     3601 attatgctaa ttgtttcagt ttttctggtt ggaaacctga aaagacttca gttggtgttt
     3661 ggataacagg agctgcgcta aaaccgagct ctttaacttg atcgacgtac tcaggttgct
     3721 catcaagatt gatttcacga taagagacat tattactgtc caagaaacgc ttggtcattt
     3781 tacattggac acaattgttt ttagaataaa cggttaccat tgtgtaactc ctcttcaaaa
     3841 tttaatacta tcttagtata tcagaaaata aaattttgtc aatgatttaa actaaatata
     3901 gtggtgttct tttgttgttt ttatatataa acacaatata tagtgtttgt attgtgaaat
     3961 agcaatgatt atcctataaa ttgtttttga agaaactata tcctgtgttt tgttatctag
     4021 gatagaagat tttcagcaag ttatctgaaa ggaaattgaa ggaaaatcca gaaaatgctt
     4081 gaaaaaaatc ctagaagatg gtataatact aaattgtaag ggttatcaca tataactcaa
     4141 aaaaagaaag aacaaaagga gagtcaaact atggcttcta aagatttcca cgtagtggca
     4201 gaaacaggta ttcacgcacg tccagcaaca ttgttggtac aaactgctag caaatttgct
     4261 tcagatatca ctcttgagta caaaggtaaa tcagttaacc ttaaatcaat tatgggtgtt
     4321 atgagtcttg gtgttggtca aggtgctgac gtaactatct cagctgaagg tgcagatgca
     4381 gatgacgcta tcgctgcaat ctcagaaaca atggaaaaag aaggattggc ataagggaaa
     4441 tgacagaaat gcttaaagga atcgcagcat ctgacggtgt tgcagttgca aaagcatatc
     4501 tactcgttca gccggatttg tcatttgaga ctattacagt cgaagataca aacgcagaag
     4561 aagctcgcct tgatgccgct ctacaggcat cacaagacga gctttctgtt attcgcgaga
     4621 aagcagtagg tacgctcggt gaagaagcag ctcaagtttt tgatgctcac ttaatggttc
     4681 ttgctgaccc agaaatgatc agccaaatca aggaaactat ccgtgcgaag aaagtgaatg
     4741 cagaagcagg tctgaaagaa gttacagata tgtttatcac tatctttgaa ggcatggaag
     4801 acaacccata catgcaagaa cgcgcagcgg atatccgcga cgtgacaaaa cgtgtattgg
     4861 caaaccttct tggtaaaaaa ttgccaaacc cagcttctat caatgaagaa gtgattgtga
     4921 ttgcgcatga cttgactcct tcagatacag ctcaattgga caaaaacttt gtaaaagctt
     4981 ttgtaaccaa cattggtgga cgtacaagcc actcagctat catggcacgt acacttgaaa
     5041 ttgctgctgt attaggtaca aacaacatca ctgaaatcgt taaagacggt gacatccttg
     5101 ctgttaacgg gatcactgga gaagtgatta tcaacccgac agatgaacaa gcggcagaat
     5161 ttaaagcagc tggtgaagcc tatgcgaaac aaaaagctga atgggcactt ttgaaagatg
     5221 ctcaaacagt gactgctgac ggtaaacact tcgagttggc tgccaatatt ggtactccaa
     5281 aagacgttga aggtgttaac aacaacggtg cagaagctgt tggactttac cgtacagagt
     5341 tcttgtacat ggattctcaa gacttcccaa ctgaagatga gcagtatgaa gcatacaagg
     5401 ctgttcttga aggaatgaac ggtaaacctg ttgtcgttcg tacaatggat atcggtggag
     5461 ataaggaact tccttacttc gatatgcctc acgaaatgaa cccattcctt ggattccgtg
     5521 ctcttcgtat ctctatctct gagactggag atgctatgtt ccgcacacaa atccgtgctc
     5581 ttcttcgtgc gtctgttcac ggtcaattgc gtatcatgtt cccaatggtt gcgctcttga
     5641 aagaattccg tgcagcgaaa gcagtctttg atgaagaaaa agcaaacctt cttgctgaag
     5701 gtgttgcagt tgcggataac atccaagttg gtatcatgat cgagattcct gcagcggcta
     5761 tgcttgcaga ccaatttgct aaagaagttg acttcttctc aattggtaca aacgacttga
     5821 tccaatatac aatggcagca gaccgtatga acgaacaagt ttcatacctt taccaaccat
     5881 acaacccatc aatcctacgc ttgattaaca atgtgatcaa agcagctcac gctgaaggta
     5941 aatgggctgg tatgtgtggt gagatggctg gtgaccaaca agctgttcca cttcttgtcg
     6001 gaatgggctt ggatgagttc tctatgtcag caacatctgt acttcgtaca cgcagcttga
     6061 tgaagaaact cgacacagct aagatggaag agtacgcaaa ccgtgccctt acagaatgct
     6121 caacaatgga agaagttctt gaacttcaaa aagaatacgt taattttgat taatcgaaaa
     6181 gtccctgcaa ctcagttaca gggacttttt ttgatatttt aaaaagaatt ttcaagaaaa
     6241 tctttcttat aaaaagtcca accttgaaaa agtagtggtc agaacaaaaa atacttaaat
     6301 ggttcataaa attcttgaca agttggagat ttaggagcaa actattaacc agttaagtaa
     6361 tagagaggag tttctgcaat ttagaaatga attgcaacta gaaatatcaa atagaaagag
     6421 agtttcgatg aaaattaata agaaatacct tgttggttct gcggcagctt tgattttaag
     6481 tgtttgttct tacgagttgg gactgtatca agctagaacg gttaaggaaa ataatcgtgt
     6541 ttcctatata gatggaaaac aagcgacgca aaaaacggag aatttgactc ctgatgaggt
     6601 tagcaagcgt gaaggaatca atgctgagca aatcgtcatc aagataacag accaaggcta
     6661 tgtcacttca catggcgacc actatcatta ttacaatggt aaggttcctt atgatgcgat
     6721 tttcagtgaa gaattactca tgaaagatcc aaactataag ctaaaagatg aggatattgt
     6781 taatgaggtc aagggtggat atgttatcaa ggtagatgga aaatactatg tttaccttaa
     6841 ggatgctgcc cacgcggata acgtccgtac aaaagaggaa atcaatcgac aaaaacaaga
     6901 acatagtcaa catcgtgaag gtgggactcc aagaaacgat ggtgctgttg ccttggcacg
     6961 ttcgcaagga cgctatacta cagatgatgg ttatatcttt aatgcttctg atatcataga
     7021 ggatactggt gatgcttata tcgttcctca tggagatcat taccattaca ttcctaagaa
     7081 tgagttatca gctagcgagt tggctgctgc agaagccttc ctatctggtc ggggaaatct
     7141 gtcaaattca agaacctatc gccgacaaaa tagcgataac acttcaagaa caaactgg
//
LOCUS       DBEJAQ010000070         6915 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.070, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000070 DBEJAQ010000000
VERSION     DBEJAQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.070"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(112..387)
                     /locus_tag="ACL297_001904"
     CDS             complement(112..387)
                     /locus_tag="ACL297_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGR0072107.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(494..1333)
                     /locus_tag="ACL297_001905"
     CDS             complement(494..1333)
                     /locus_tag="ACL297_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGR0072108.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1475..2323
                     /locus_tag="ACL297_001906"
     CDS             1475..2323
                     /locus_tag="ACL297_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGR0072109.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2416..3333)
                     /locus_tag="ACL297_001907"
     CDS             complement(2416..3333)
                     /locus_tag="ACL297_001907"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGR0072110.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3812..3960
                     /locus_tag="ACL297_001908"
                     /pseudo
     CDS             3812..3960
                     /locus_tag="ACL297_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
     gene            complement(4286..4579)
                     /gene="rpmA"
                     /locus_tag="ACL297_001909"
     CDS             complement(4286..4579)
                     /gene="rpmA"
                     /locus_tag="ACL297_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGR0072111.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4596..4940)
                     /locus_tag="ACL297_001910"
     CDS             complement(4596..4940)
                     /locus_tag="ACL297_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGR0072112.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4956..5270)
                     /gene="rplU"
                     /locus_tag="ACL297_001911"
     CDS             complement(4956..5270)
                     /gene="rplU"
                     /locus_tag="ACL297_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGR0072113.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5306..5393)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5452..6156)
                     /locus_tag="ACL297_001912"
     CDS             complement(5452..6156)
                     /locus_tag="ACL297_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HGR0072114.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6558..6857
                     /locus_tag="ACL297_001913"
     CDS             6558..6857
                     /locus_tag="ACL297_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGR0072115.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2177 a         1405 c         1151 g         2182 t
ORIGIN      
        1 aatattttcg gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa
       61 gcctactgac caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac
      121 agcgtcttta agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat
      181 ttctttacca gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt
      241 accaaaaccg atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa
      301 tacagcttca actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt
      361 agcgatcaaa tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac
      421 aaatataatc atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat
      481 tttatttatt tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt
      541 gagtttgaat aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta
      601 agctttcttt catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg
      661 ctacagcacg ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag
      721 ttccacgacc tttaaccatt ggctgcaatt catggtcttt catttgcatg acaacacgga
      781 tattgagaag agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat
      841 tttccaaagt tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag
      901 ataaaattgc ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga
      961 atttcagggc ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc
     1021 tagcaccttg gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa
     1081 gaatctggcc accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg
     1141 gctgacttgt tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt
     1201 ctttcaaatc cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg
     1261 taatatctaa ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct
     1321 taatttttgt catagtatca atctttctat tttaggattc agattggttt ccttacttct
     1381 aattatatca aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt
     1441 tgatataatc aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt
     1501 taccatttta ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca
     1561 tcatctcttt gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat
     1621 ccctgtttcc ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaagat
     1681 ctttggagcc aaatccctct attctagttt actaggaacc ttagctttgt ccgcctggtt
     1741 agcttttttt gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc
     1801 ccttatagcg ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac
     1861 aactggcgga actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg
     1921 aaaactgctc tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga
     1981 tttgagattg gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt
     2041 gattggtgaa ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca
     2101 acttgctaag gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg
     2161 ctactatagt aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt
     2221 gaaaacgaag gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc
     2281 ccatgaaatc ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg
     2341 acctcaaaag ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt
     2401 tatttactca agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc
     2461 cactaattgg tcaactgagt caaatttggt catatcacga atgcgatcaa gccaataaac
     2521 catgacggtt tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc
     2581 ttcttctcca tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct
     2641 ttgaatctca acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag
     2701 cactaaattc gctgtcggat aaccaattgt acgaccacga gcattaccat gaaccaccat
     2761 acctcttgat ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa
     2821 aatagcttga cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac
     2881 aatgataact tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc
     2941 aaatgtataa tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc
     3001 aaagaattct tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc
     3061 ttctacacct tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa
     3121 caaatctgga tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac
     3181 gataggcaat aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc
     3241 cttatgtatg ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc
     3301 tttttggttt tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta
     3361 tttctggaac agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa
     3421 aagcacttta ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg
     3481 taggacatct tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc
     3541 atactatgtt atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat
     3601 tggtgccaaa aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa
     3661 tgagacaact tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc
     3721 actttccctg atgacaaaga aaaaagccaa aacagcaaag aaaaactaga tgactgcttt
     3781 ccaacaactc ccatctagtg tgcttcagac tgggctattt ttctctccat ctgttagctt
     3841 ggattctcag accgtttcag ctaaagaata tcttttccct tatcagaagg aacggctcaa
     3901 accattcaga caagtgaagg gacgacaagc caatatttga aaccagatag cagttcttat
     3961 agtcaattga aataaaatct gaagaaatcg agtaggaaac tcatatcaat ttttaacagt
     4021 gttctattcc agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag
     4081 gctgtacttg agtacggcaa ggtgaaattg acgtggtttg aatttgattt tcgaagagta
     4141 tcaatccact atacatatta aaaagtatgg gtatcaaaat aaaaaaacag gctctccgaa
     4201 aactcggaaa gccttatttt aagctgtttc tagcttcctc acctttacat ttcaaggttc
     4261 gggataaaag gttcaatgaa cctttttatt ttgcgattgg gtaaacagaa acttgttttt
     4321 tatcgcgtcc tttacgttca aagcgtacta cgccttcaac tttagcgaac aaagtatcgt
     4381 ctccaccacg accaacgttt acacctggat agatgtgtgt accacgttga cggtaaagga
     4441 ttgatccacc tgttacagtt tgtccgtcag ctgctttagc tccaagacgt tttgcttgtg
     4501 aatcacgtcc gtttgatgta gaacctccac cttttttgtg ggcgaaaagt tgcaagttgt
     4561 taagagtcat ttttaacata atgttttcct ccgtgttagt tttctgtgat aactctggtt
     4621 tggacgaact cagaatagtt ctccgataag tttgccatac ctaagaaaaa tgattcaaag
     4681 aataactggg tcatttctct ctggtgtgaa ggaagatctt ttggtatttc aaccatcaga
     4741 tagccacctt catcttcgtt taattctagg attggttcat agcctgcaaa tttctcaata
     4801 gaattgataa agttaatggc aagcgtagaa accgatgcac acacgacatc taagccgtat
     4861 tcgccactct cggcgtgtcc agtaatttcc gcactcctca gctcgccatc ttcggctctc
     4921 tcaaagactg cctgtatcat gtgttctcct taaaattaag cgttgattgc gttgatgaca
     4981 acttttgtat atggttgacg gtgaccttgt ttacggtggc tacctttttt aggtttgtac
     5041 ttgtaagtaa ccactttctt ttgttttcct tgtttttcaa cagttccaac tacagtagct
     5101 ccagcaacaa gtggagttcc gacaacagtg ttttcaccac caacaagaac aacttcgtta
     5161 aaagtaactt cttgaccagc ttcaacgttc aatttttcaa cgtaaactgc ttgaccaact
     5221 tcaactttaa cttgttttcc gccagttttg ataattgcgt atgtgctcat tatgcacctc
     5281 ctatgatttt tatgggtttc cccgattttt tgtgaagact cgcctagcat cgtgggacga
     5341 accacttaaa cttgaataat caagcaacga tgttcgtgcg gttgcacagg atcgtgcata
     5401 gtcaactctt caagtatagc atatctccta ttttcttaca agtaataaca cctaaaatga
     5461 agctttttct tttacttttt tctgccaaga ggcaaaaagc atgctgaggt aaaaaacgct
     5521 catcataata ggaacaccaa gaatggtctt ttcatgatag aaaatcgtca aataggctga
     5581 aaagacaacg ccaaggacaa aactactaag caggctaaca aatatgaatc cttcacgcaa
     5641 aaaaggagtg tgcttggttc ggaaataatc tccaaaagcc agcatggtcc gtttgatatt
     5701 ccctgtcata aaagcgttat tataggcaat acccgacact tctccaaaag cagttgtcac
     5761 cagtcccata cagaaggcca agggcggcac tagatagata ttatccacag tttgcggcac
     5821 aaaagcaata atgattgata agattgccaa gggaatcaag gacagaatag gttttttcac
     5881 aattctcaat ttttccttat aaatcgttaa taaaaagact cccatcataa acgctagcaa
     5941 ggtgagaacc ttgtccctaa catccgaaac attattttta attaattcta ctgaaagaaa
     6001 gacaacattt ccagtttgtc cagctacaag ggtattcccg cgaacaataa aagtgtaagc
     6061 atccacatat ccagcacaaa acgtcaaaaa aagtgctaac cttttagact gacgtgatat
     6121 ttttcttata ggtaataacc tcattttacc tcccattgta ttttctctta gaaatattgt
     6181 accattttct ttctaaaaaa tcgtaggcta ccatttagat tttactatta gcataaaaat
     6241 aataatagac aactatttat ccaaaaatag atagatgtaa catgtttgca aacaaagcat
     6301 acgaaccttt agtaaaatca tttccatgaa actagaatag agccctctta gcaaaaatca
     6361 ttattttaat ttatttctaa tcactccttg acataaataa ctctcaccaa taaaagacta
     6421 tgtcttaaaa aaatggtata ataaaatcaa tacttgggct tgatggctat gctactaata
     6481 acaattagga gagaaaatca ggcacttgtt aacaacaagg attatcccct tgagatgaaa
     6541 ggaactttag aaatcttatg atgaacatgc aaaacatgat gcgtcaagca caaaaacttc
     6601 aaaaacaaat ggaacaaagc caagctgaac ttgctgctat gcaatttgtt ggcaaatctg
     6661 ctcaagatct tgtccaagcg accttaactg gcgataagaa agttgtcagc attgatttca
     6721 atccagctgt cgttgaccca gaggaccttg agactctttc tgatatgacc gttcaagcca
     6781 tcaactctgc tcttgaacaa atcgatgaaa ctaccaagaa aaaactgggt gctttcgctg
     6841 ggaaattacc tttctaaaaa caaggagcta gaacaatgct tgtcgataac aaaggctaag
     6901 aaaggtgcaa aaatg
//
LOCUS       DBEJAQ010000071         6746 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.071, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000071 DBEJAQ010000000
VERSION     DBEJAQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.071"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            1..168
                     /locus_tag="ACL297_001914"
                     /pseudo
     CDS             1..168
                     /locus_tag="ACL297_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            335..535
                     /locus_tag="ACL297_001915"
     CDS             335..535
                     /locus_tag="ACL297_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072116.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            577..982
                     /locus_tag="ACL297_001916"
                     /pseudo
     CDS             577..982
                     /locus_tag="ACL297_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
     gene            1213..1920
                     /gene="ccdA2"
                     /locus_tag="ACL297_001917"
     CDS             1213..1920
                     /gene="ccdA2"
                     /locus_tag="ACL297_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGR0072117.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1931..2488
                     /gene="sdbB"
                     /locus_tag="ACL297_001918"
     CDS             1931..2488
                     /gene="sdbB"
                     /locus_tag="ACL297_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HGR0072118.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2862..4253
                     /locus_tag="ACL297_001919"
     CDS             2862..4253
                     /locus_tag="ACL297_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGR0072119.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4396..5313
                     /gene="adcAII"
                     /locus_tag="ACL297_001920"
     CDS             4396..5313
                     /gene="adcAII"
                     /locus_tag="ACL297_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HGR0072120.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5321..>6746
                     /gene="phtD"
                     /locus_tag="ACL297_001921"
     CDS             5321..>6746
                     /gene="phtD"
                     /locus_tag="ACL297_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HGR0072121.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPVQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVRDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEKNGVSRYIPAKNLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFE"
BASE COUNT         2079 a         1243 c         1421 g         2003 t
ORIGIN      
        1 aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac aaatggtgaa
       61 ttaatcgctc caatgactta cgaagagatg atgacgagcg acttttttga agtatggttt
      121 cagaagtttc tcttaccaac attaaccaca ccatcggtta ttatagtaaa atgaaataag
      181 aacaggacaa atcgatcagg acagtaaaat cgaattctaa caatgtttta gaagtccata
      241 tgtactattc tagtttcaat ataccataac tgttcctagt caacctgcaa gccctgtccc
      301 agctccaagc tcagctccga ctcaagatcc tagagtggta gcaagcgctt cggctagctc
      361 aacgagcaca caagctcaag agcaagttga caagtctgaa cttcgtgcct tgagtcaaga
      421 gttagaccaa cgcttgaaag ctttggcaac agtatctgat ccgaaaatcg atgcaaccaa
      481 ggctgtcctc ctagatgctc aaaaagctcc ggaagatagt gccttgacag agtaaggtct
      541 gcatactgca gtagagtctg tgaaggctgg gctgccttgg actctctcaa agatgtgaaa
      601 gcaaatgcta gcgacagcaa gcctacacag gacaagaagg atgcaaaaca aggaacggaa
      661 gatagtaagg attcagataa gatgactgaa acaaactcag ttccagcagg agtgattgtg
      721 gtcagtctac ttgccctcct aggcgtgatt gccttctggc taattcgccg taagaaagag
      781 tcagaaatcc agcaattaag cacggaattg accaaggttc taggacagct agatgcagaa
      841 aaaagcggat aaaaaagtcc ttgccaaagc ccaaaacctt ctccaagaaa cccttgattt
      901 cgtgaaagaa gaaaatggct cagcagagac agaaactaaa ctagtagagg agcttaaagc
      961 aatccttgac aaactcaagt aaaacatgat ttatgaaatg tcagacctaa aagacatttt
     1021 cgttcacaaa agcagcgacg agtaatcctt gtcgttgctt tttggctatt ttgggggata
     1081 agatccgcct tgactagcaa aagaaaagat tggtccaata gtgcccgata cgattttgcg
     1141 tagcagaaag cttacaaata aaattgaata tagtaaaata ggattagaat tattaagaaa
     1201 gttggttctt tattggaaac gatagtattt ttaatctctg tttttctagc aggtgtttta
     1261 tccttttttt ctccttgtat ttttcctctt ctgccagtct atgctgggat tttattggat
     1321 gatcaagaaa gtgcaaaaag cttttctttg tttgggagaa aggttctctg gtcaggcttg
     1381 attcgaacac tttgctttat cgctggtatc tctctcattt tctttattct aggctttggt
     1441 gctggttact ttggtcatat tctctatgca aattggtttc gatatggcat gggagctatt
     1501 attatcattt tgggtcttca ccagatggaa atttttcatt tgaagaaatt agaagttcaa
     1561 aaaagtttta cctttaaaaa atcagactct aatcgttatt ggtcagcttt tttacttggt
     1621 attaccttta gctttggttg gacaccttgt attggtccag ttttaagttc tgttttagca
     1681 cttgcggctt ctggaggcaa tggcgcttgg caaggagcga tttatactct catttacact
     1741 ctgggcatgg cccttccttt cttggtattg gcactagctt caggtctagt catgccatat
     1801 tttagtaaaa tcaagcgtca tatgatgcta ctaaagaaaa ttggtggttt cctcattgtt
     1861 ttaatgggaa ttttgttact attaggacaa gtaaatgttc tagctggaat ttttgaataa
     1921 aggaggacaa atgaaaaaag taatgtttgc tggcttaagt ctcttgtcat tagttgtatt
     1981 gatggcctgt ggtgaggaag aaactaaaaa gactcaagca gcacaacagc caaaacaaca
     2041 aacgactgta caacaaattg ctgttggaaa agatgctcca aacttcacat tgcaatccat
     2101 ggatggcaaa gaagttaagt tatctgattt taagggtaaa aaggtttact tgaagttttg
     2161 ggcttcatgg tgtggtccat gcaagaaaag tatgccagag ttgatggaac tagcggcgaa
     2221 accagatcgt gatttcgaaa ttcttactgt cattgcacca ggaattcaag gtgaaaaaat
     2281 tgttgagcaa ttcccacaat ggttccagga acaaggatat aaggatatcc cagttcttta
     2341 tgataccaaa gcaaccacct tccaagctta tcaaattcga agcattccta cagaatattt
     2401 aattgatagc caaggaaaga ttggaaagat tcaatttggt gctatcagta atgcggatgc
     2461 agaagcagca tttaaagaaa tgaactagaa tcgataaaat ctgattacaa gatgtagtca
     2521 gattttggta acttctaaaa ctaacttcca gtttcccaca acctagcttt tagtatgaga
     2581 aaaacgcgtg acatcagtgg aaatccgtct tagactaact tccactggtt ttctttttct
     2641 tgatatataa gggttcaaaa gcgaaaagac tcagaaaaag tgcacgacaa atagccctaa
     2701 aacagagtcg tcttagagta acttccagtt ttgggagaga gatggaagtt actttgagaa
     2761 gttacggtag tcagattttt ttagaaaaac attttataaa tattcacata tctcctatat
     2821 ttatggtaaa atagaatcat cagtttattt tggagtcaaa gatgaatata tttagaacaa
     2881 agaatgttag tttagataaa acagagatgc ataggcattt gaagttatgg gatttgattt
     2941 tgctgggtat cggagccatg gtagggacag gcgtctttac aatcacaggt actgcagctg
     3001 caacacttgc tggcccagcc ctagtgattt caatcgttat ttctgccttg tgtgtgggat
     3061 tatcagccct cttttttgca gaatttgcct cgcgagtacc cgctacagga ggtgcctata
     3121 gttacctcta tgctatctta ggagaattcc ctgcctggtt ggctggttgg ttaaccatga
     3181 tggagttcat gacagccata tcaggcgtag cttcgggttg ggcagcttat tttaaaggct
     3241 tgctctctca atacgggata gcctttcctc aggctttaaa tggtaccttt aatcctcaag
     3301 cagggacatt tgttgattta ttgcctattc ttgtcttggt cttggtgact tcgcttgttt
     3361 tactaaatgc caaggcagcc ttacgcttta attcgattct agttattttg aaattttccg
     3421 ctttagctct ctttgtttta gtaggaattt ggaatattaa gtttgataat tggagcaatt
     3481 ttgctcctta cggttttgga caaatctatg gagctagcac tggtattatg gctggtgcgt
     3541 ccttgatgtt cttcggtttt ttgggatttg aatccatctc tatggctgta gatgaagtca
     3601 agactcctca aaaaaatatt cctagaggga ttgtcttatc gctttctatc gtaaccattc
     3661 tctatgcctt ggtgaccctt gttttgactg gtgtggttca ctatagtcat ttaaatgtcg
     3721 atgatgccgt tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc aactatgtgt
     3781 cattagtggc tatcttgacc ttgattacag tttgtatttc gatgacctat gccctatcgc
     3841 gtatgattta cagtttagca cgtgacggct tagtgcctgc tgcctttaag gaactgacga
     3901 agactagcaa gataccaaag aatgctacta tcttgacagg tctagcttca gcagtagcag
     3961 caggaatgtt cccactagcc agtattgcag cctttttaaa tatttgtacc ttagcctact
     4021 tgatcatgtt ggcttatggt ctgattcgct tacggaaaga aaaaggaatg cccaaagctg
     4081 gggaatttaa aacaccattg gtacctctgt taccaatttt atcgatcatc atttgtttat
     4141 ccttcatgtt acagtataat atgaatacct ggattgcttt cctagttgcg ttgttagtag
     4201 gaagtattat ttacttcact tatggctata agcattctac catagaagaa taaagtgaaa
     4261 gtctattgag ttgttgacac tcagcagatt tttatatgtg aaagaaattt caaatttttt
     4321 ctaaaaatat cttgacagat taaattttca ggagtagaat atttactagt taattaaatg
     4381 gttaaggagt tgttcatgaa gaaacaaaat ttatttttag tcctgttaag tgtctttctt
     4441 ttgtgcttgg gggcttgtgg tcaaaaggaa agtcagacag gaaaggggat gaaaattgtg
     4501 accagttttt atcctatcta cgctatggtt aaggaagtat ctggtgactt gaatgatgtt
     4561 cggatgattc agtcaagtag tggtattcac tcctttgaac cttcggcaaa tgatatcgca
     4621 gccatctatg atgcagatgt ctttgtttac cattctcata cactcgaatc ttgggcagga
     4681 agtctggatc caaatctaaa aaaatccaaa gtgaaggtct tagaggcttc tgagggaatg
     4741 accttggaac gtgtccctgg actagaggat gtggaagcag gggatggagt tgatgaaaaa
     4801 acgctctatg accctcacac atggctagat cctgaaaaag ctggagaaga agcccaaatt
     4861 atcgctgata aactttcaga ggtggatagt gagcataaag agacttatca aaaaaatgcg
     4921 caagccttta tcaaaaaagc tcaggaattg actaagaaat tccaaccaaa atttgaaaaa
     4981 gcgactcaga aaacatttgt aacacaacat acagcctttt cttatctagc gaagagattt
     5041 gggcttaatc aacttggtat tgcaggtatc tctcctgaac aagaaccaag tccacgacaa
     5101 ctaacagaaa ttcaggaatt tgttaagacc tataaggtta aaacgatttt tacagaaagt
     5161 aacgcttctt caaaagtagc tgaaactctt gtcaaatcaa caggtgtggg tcttaaaact
     5221 ctgaatcctt tagagtcaga cccacaaaat gacaagacct atttagaaaa tcttgaagaa
     5281 aatatgagta ttctagcaga agaattaaag tgaggaaaga atgaaaatca ataaaaaata
     5341 tctagcaggt tcagtggcag tccttgccct aagtgtttgt tcctatgaac ttggtcgtca
     5401 ccaagctggt caggttaaga aagagtctaa tcgagtttct tatatagatg gtgatcaggc
     5461 tggtcaaaag gcagaaaatt tgacaccaga tgaagtcagt aagagagagg ggatcaacgc
     5521 cgaacaaatt gttatcaaga ttacggatca aggttatgtg acctctcatg gagaccatta
     5581 tcattactat aatggcaagg ttccttatga tgccatcatc agtgaagaac ttctcatgaa
     5641 agatccgaat tatcagttga aggattcaga cattgtcaat gaaatcaagg gtggctatgt
     5701 gattaaggta gacggaaaat actatgttta ccttaaagat gcggcccatg cggacaatat
     5761 tcggacaaaa gaagagatta aacgtcagaa gcaggaacgc agtcataatc ataactcaag
     5821 agcagataat gctgttgctg cagccagagc ccagggacgc tacaccacag atgatggtta
     5881 tatctttaat gcttctgata tcattgagga cacgggtgat gcttatatcg ttcctcacgg
     5941 cgaccattac cattacattc ctaagaatga gttatcagct agcgagttag ctgctgcaga
     6001 agcctattgg aatgggaagc agggatctcg tccttcttca agttctagtt ataatgcaaa
     6061 tccagttcaa ccaagattgt cagagaacca caatctgact gtcactccaa cttatcatca
     6121 aaatcaaggg gaaaacattt caagcctttt acgtgaattg tatgctaaac ccttatcaga
     6181 acgccatgtg gaatctgatg gccttatttt cgacccagcg caaatcacaa gtcgaaccgc
     6241 cagaggtgta gctgtccctc atggtaacca ttaccacttt atcccttatg aacaaatgtc
     6301 tgaattggaa aaacgaattg ctcgtattat tccccttcgt tatcgttcaa accattgggt
     6361 acgagattca agaccagaac aaccaagtcc acaatcgact ccagaaccta gtccaagtcc
     6421 gcaacctgca ccaaatcctc aaccagctcc aagcaatcca attgatgaga aattggtcaa
     6481 agaagctgtt cgaaaagtag gcgatggtta tgtctttgag aagaatggag tttctcgtta
     6541 tatcccagcc aagaatcttt cagcagaaac agcagcaggc attgatagca aactggccaa
     6601 gcaggaaagt ttatctcata agctaggagc taagaaaact gacctcccat ctagtgatag
     6661 agaattttac aataaggctt atgacttact agcaagaatt caccaagatt tacttgataa
     6721 taaaggtcga caagttgatt ttgagg
//
LOCUS       DBEJAQ010000072         6620 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.072, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000072 DBEJAQ010000000
VERSION     DBEJAQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.072"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(1..192)
                     /locus_tag="ACL297_001922"
                     /pseudo
     CDS             complement(1..192)
                     /locus_tag="ACL297_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            390..2156
                     /gene="patB"
                     /locus_tag="ACL297_001923"
     CDS             390..2156
                     /gene="patB"
                     /locus_tag="ACL297_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HGR0072122.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2295..2984
                     /locus_tag="ACL297_001924"
     CDS             2295..2984
                     /locus_tag="ACL297_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGR0072123.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            2988..3167
                     /locus_tag="ACL297_001925"
     CDS             2988..3167
                     /locus_tag="ACL297_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072124.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3154..4503
                     /locus_tag="ACL297_001926"
     CDS             3154..4503
                     /locus_tag="ACL297_001926"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGR0072125.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4627..6087
                     /gene="gltX"
                     /locus_tag="ACL297_001927"
     CDS             4627..6087
                     /gene="gltX"
                     /locus_tag="ACL297_001927"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGR0072126.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            6101..6532
                     /locus_tag="ACL297_001928"
     CDS             6101..6532
                     /locus_tag="ACL297_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGR0072127.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT         2086 a         1267 c         1419 g         1848 t
ORIGIN      
        1 agaatccagt ccagcgaaag cttgtaattg agcaggatta tcaaaggcat gaatatttcg
       61 aatctcggct aaaatgaccg cccctaaacg acccccaatc ccagtaaccg tcgtgatgac
      121 cgagttgaac tcagccatca agtcattgac acattttttc gccttgtcaa tgagcctctt
      181 gtaatgtttg atgttttcat tacaagagat aaaacgttta tgcgttatca aactcattac
      241 caattgaaac aaaaagctgt ggttagatcc tttcggaaat tgtcaagcga ttggaggaaa
      301 tgaactaatc cacagcggct tattccaagt ataccacttg ggctttggca gtagctaact
      361 gcgctaaata taatataagg aggagtaaaa tgaagacagt tcaatttttt tggcattatt
      421 ttaaggtcta caagttctca tttgtagttg tcatcctgat gattgttctg gcgacttttg
      481 cccaagccct ctttccagtc ttttctggac aagcggtgac gcagctagcc aatttagttc
      541 aagcttatca aaatggcaat ccagaacttg tatggcaaag cctatcagga atcatggtca
      601 atcttggcct gctggttttg gttctattta tctctagtgt aatatacatg tgtctcatga
      661 cgcgcgtgat tgcagaatcg accaacgaga tgcgcaaagg cctctttggt aagcttgctc
      721 agttgacggt ttctttcttt gaccgtcgac aagatggcga tatcctgtct cattttacca
      781 gtgatttgga taatatcctc caagccttta acgaaagctt gattcaggtc atgagcaata
      841 ttgttttata cattggtctg attcttgtca tgttttcgag aaatgtgacg ctggctctca
      901 tcaccattgc cagcacccca ttggctttcc ttatgctgat tttcatcgtg aaaatggcac
      961 gtaaatacac caacctccag cagaaagagg tagggaagct caacgcctat atggatgaga
     1021 gcatctcagg ccaaaaagcc gtgattgtgc aaggaattca agaggatatg atggcaggat
     1081 ttcttgaaca aaatgagcgc gtgcgcaagg caacctttaa aggaagaatg ttctcaggaa
     1141 ttcttttccc tgtcatgaat gggatgagcc tgattaatac agccatcgtc atctttgctg
     1201 gttcggctgt acttttgaat gataagtcta ttgaaacaag tacagcccta ggtttgattg
     1261 ttatgtttgc acaattttca cagcagtact accagcctat tatccaagtt gcagcgagtt
     1321 ggggaagcct tcagttggcc tttactggag ctgaacgaat tcaggaaatg tttgatgcag
     1381 aggaggaaat ccgacctgaa aaggctccaa ccttcactaa gttgcaagaa agtgttgaaa
     1441 tcagtcatat cgatttttca tacttgcctg ataaacctat tttgaaagat gtcagcattt
     1501 ctgcccctaa aggccagatg acagcagttg ttgggcctac aggttcagga aaaacgacta
     1561 ttatgaacct catcaatcgc ttttatgatg ttgatgctgg tggtatttat tttgatggta
     1621 aagacattcg tggctatgac ttagatagtc ttagaagcaa ggtgggaatt gtattgcaag
     1681 attcggtctt gtttagcgga acgattcgag acaatatccg atttggtgtg ccagatgcta
     1741 atcaggaaat ggttgaggta gcagcaaaag caacccacat tcacgactat atcgaaagtt
     1801 tgcctgataa gtacgatact cttattgatg atgaccagag catcttttca acagggcaga
     1861 agcaattgat ttcaatcgct cgaaccctga tgacagatcc agaagttctc attctcgatg
     1921 aagcaacttc aaacgtagat acggtgacgg aaagcaagat tcagcatgcc atggaggtgg
     1981 ttgtagcagg tagaactagt ttcgtcattg cccaccgctt gaaaaccatt ctcaatgcag
     2041 atcagattat tgtccttaaa gatggagaag tcattgaacg tggtaaccac catgaacttt
     2101 tgaagctagg tggcttttat tcagaactct atcacaatca atttgttttc gaataagaaa
     2161 gaagttgtcc tatgtgggca gctttttctt gtccataaaa aatgtttatc acagccttaa
     2221 aaaaaacata ttagacgaaa gtcattttga gtgatatgat aggactatcg ttagtattcg
     2281 aaaggagagg catcatggct agaacggttg taggagttgc tgcaaatcta tgtcccgtag
     2341 acgcagaagg caaaatcatt cattcatctg tatcttgtag attcgcagag atcattcgtc
     2401 aagtcggtgg tctcccttta gtcattcctg ttggtgatga gtcagttgta cgtgattatg
     2461 tggaaatgat tgacaaactc attttgacag gaggccaaaa tgttcatcct cagttttatg
     2521 gagagaaaaa gaccgtcgaa agcgatgatt acaatctggt ccgtgacgaa tttgaattgg
     2581 cactcttgaa ggaagcgctt cgtcagaata aaccaattat ggcaatctgt cgcggtgtcc
     2641 aacttgtcaa tgttgccttt ggtggaaccc tcaatcaaga aatcgaaggt cactggcaag
     2701 gactaccttt cgggacatct cactctattg aaacagtaga aggaagcgtg gtggctaagc
     2761 tatttgggaa agaaagtcag gtcaactcag tccatcgtca aagcattaaa gatttggcac
     2821 ctaatttccg tgtaactgct attgattcaa gagaccaaac tattgaagcg attgagtcta
     2881 tcgacgagca ccgcattatc ggtttacagt ggcatccaga gtttctggtt aatgaagaag
     2941 atggcaattt agaattattt gagtatttat tgaatgaact gtaacgattg gaacatctgg
     3001 tcgttctttc ttttattttt tcaaaatttt tagaatgtgg cattttacgc aaacgtttga
     3061 attctgataa aaattggaga aattcgacaa aaaaacttga aaaaaacgaa ggtaagcgtt
     3121 atgatagaaa agaagaaata ttggaggaag aacatgtcac atattaaatt tgattattca
     3181 aaagttttag acaaatttgt tgcaccacat gaagtggaat acatgcaatc acaagtaaca
     3241 gcagcagatg aattgatccg taaaggaact ggtgctggta gcgacttctt gggatggttg
     3301 gaccttcctg aaaaatacga ccgcgaagaa tttgaccgca tcttgaaagc tgctgagcaa
     3361 atcaagtcag acagcgatgt tttggttgtg atcggtattg gtggatctta ccttggtgca
     3421 aaagcagcaa tcgacttctt gaatcaccat tttgctaact tgcaaacaaa agaagaacgc
     3481 aaagctccac aaatccttta cgcaggaaac tcaatctcat ctacttacct tgctgacttg
     3541 gtagagtatg ttgcagacaa agacttctca gtaaacgtaa tttctaaatc aggtacaaca
     3601 actgaaccag cgattgcttt ccgtgtcttt aaagaactct tggttaagaa atacggtcaa
     3661 gaagaagcta acaaacgtat ctatgcaaca actgaccgcc aaaagggtgc tgttaaggtt
     3721 gaagcagacg ctaacggttg gggaacattt gttgttccag atgatatcgg tggacgcttc
     3781 tcagtattga cagccgttgg tttgcttcca atcgcagcat caggagctga catcaaagct
     3841 cttatggaag gtgcgaatgc agctcgcaaa gactacactt cagacaaaat ctctgaaaac
     3901 gaagcttacc aatacgcagt cgttcgtaac atcctttatc gtaaaggcta tgcaactgag
     3961 atcttggtaa actatgagcc atcacttcaa tacttctcag aatggtggaa acaattggct
     4021 ggtgaatcag aaggaaaaga ccaaaaaggt atctacccaa cttcagccaa cttctcaact
     4081 gacttgcact cacttggtca atttatccaa gaaggaactc gtatcatgtt tgaaacagtt
     4141 gtccgtgttg acaaacctcg taaaaacgtg cttattccta ctttggaaga agaccttgac
     4201 ggacttggtt accttcaagg aaaagacgtt gactttgtaa acaaaaaagc aactgacggt
     4261 gttcttcttg cccacacaga tggtgatgta ccaaacatgt atgtgactct tccagagcaa
     4321 gacgctttca ctcttggtta cactatctac ttcttcgaat tggcaattgc cctttcaggt
     4381 tacttgaatg ctatcaaccc atttgaccaa ccaggtgttg aagcttataa acgtaacatg
     4441 tttgcccttc ttggaaaacc aggatttgaa gaattgagca aagaacttaa cgcacgtcta
     4501 taatagaaga aaagagtggt ttgcccactc tttttactct ctttatccat agaaattgga
     4561 ctcagccaag acttgtgata taatatagaa agcaaaaagg cagacgccta gataatagga
     4621 gaaactatgt caaaagatat ccgcgtacgt tacgcaccaa gtccaacagg actactacac
     4681 atcggaaatg cccgtacagc attgtttaac tacttgtatg cgcgccatca tggtggaaca
     4741 tttctcatcc gtatcgaaga tactgaccgt aaacgccatg tcgaggatgg tgaacgttca
     4801 caacttgaaa accttcgctg gttaggcatg gattgggatg aaagtccaga atcacatgag
     4861 aattatcgcc agtctgagcg tttggacttg tatcaaaaat acattgacca actattagct
     4921 gaaggaaaag cctataaatc ttacgttaca gaagaagagt tggcagctga acgtgaacgt
     4981 caagaagtag ctggcgaaac accacgctac atcaatgaat accttggtat gagtgaagaa
     5041 gaaaaagcgg cttatatcgc agaacgtgaa gcagctggta tcataccaac agttcgtttg
     5101 gctgtcaatg agtcaggtat ctacaagtgg catgatatgg tcaaaggtga tatcgaattt
     5161 gaaggtggca atatcggcgg tgactgggtt atccaaaaga aagacggtta cccaacttac
     5221 aactttgccg ttgttatcga tgaccacgat atgcaaatct ctcatgttat ccgtggagat
     5281 gaccatattg ctaatacacc aaaacagctt atggtctatg aagctcttgg ttgggaagct
     5341 ccagagtttg gtcacatgac cttgattatc aactctgaga ctggtaagaa gttatctaaa
     5401 cgtgatacta atacacttca atttatcgaa gattaccgaa agaaaggtta ccttccagaa
     5461 gcagtcttta actttattgc tcttcttggt tggaacccag gtggtgaaga tgagattttc
     5521 tctcgtgaag aactcattaa actttttgat gaaaaccgcc tcagcaagtc accagcagcc
     5581 tttgatcaga aaaaattaga ctggatgagc aatgattata tcaagaatgc agaccttgaa
     5641 accatctttg aaatggcaaa accattctta gaggaagcag gccgtttgac tgacaaggct
     5701 gaaaaattag ttgagctcta taaaccacaa atgaaatcag tagatgagat tatcccattg
     5761 acagatcttt tcttctcaga tttcccagaa ttgacagaag cagagcgcga agtcatgacg
     5821 ggtgaaacag ttccaacagt tcttgaagca ttcaaagcaa aacttgaagc gatgacagat
     5881 gatgaatttg tgacagaaaa tatcttccca caaattaaag cagtccaaaa agaaacaggt
     5941 attaaaggga aaaatctttt catgcctatt cgtatcgcag tttcaggcga aatgcatggg
     6001 ccagaattac cagatacaat tttcttgctt ggacgtgaaa aatcaattca gcatatcgaa
     6061 aacattctaa aagaaatctc taaataaaga ggatacaata atggacatct gggaaaagat
     6121 gtacgaagaa gcacagaaac tatacaatcc acatgaagta tcagactttg tttatgccaa
     6181 tcatgttgta gctgcagtag aagcaggaga tggacaaata tttacaggat tctgtatgga
     6241 gggaacctgt ggtgttttcc atctctgcgc agaacgggcg accctcttca atatgtacca
     6301 attttcagga caaactaagg ttaagaaagt attagccttt cgagacaaac caccttatgg
     6361 tggaagttca gccatgcctt gcggtgcttg tagagaattc cttttagagc ttaatgctga
     6421 aaataaagat gcagaattca tgatggacta taatataaga aaaacagtta aagtcgcaga
     6481 actaatccct tattggtggg gagaagaacg tgcttctaag tttaatgagt gatagaagag
     6541 tcagtataat tctggctctt tttactataa attgaaaaaa taaaaaaaat tagaaaaagt
     6601 agttgacaaa atttgaaaag
//
LOCUS       DBEJAQ010000073         6476 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.073, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000073 DBEJAQ010000000
VERSION     DBEJAQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.073"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            110..310
                     /locus_tag="ACL297_001929"
     CDS             110..310
                     /locus_tag="ACL297_001929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGR0072128.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            345..1061
                     /gene="atpB"
                     /locus_tag="ACL297_001930"
     CDS             345..1061
                     /gene="atpB"
                     /locus_tag="ACL297_001930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGR0072129.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            1075..1569
                     /gene="atpF"
                     /locus_tag="ACL297_001931"
     CDS             1075..1569
                     /gene="atpF"
                     /locus_tag="ACL297_001931"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGR0072130.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            1569..2105
                     /locus_tag="ACL297_001932"
     CDS             1569..2105
                     /locus_tag="ACL297_001932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGR0072131.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            2120..3625
                     /gene="atpA"
                     /locus_tag="ACL297_001933"
     CDS             2120..3625
                     /gene="atpA"
                     /locus_tag="ACL297_001933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGR0072132.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            3641..4519
                     /locus_tag="ACL297_001934"
     CDS             3641..4519
                     /locus_tag="ACL297_001934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGR0072133.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            4605..6011
                     /gene="atpD"
                     /locus_tag="ACL297_001935"
     CDS             4605..6011
                     /gene="atpD"
                     /locus_tag="ACL297_001935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGR0072134.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            6022..6441
                     /locus_tag="ACL297_001936"
     CDS             6022..6441
                     /locus_tag="ACL297_001936"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGR0072135.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
BASE COUNT         1924 a         1213 c         1431 g         1908 t
ORIGIN      
        1 aaatggaaat aatttttatt aaacgcttcc aatagcggtt aaaagttgac aaatttaaat
       61 tttaggacta cactaagtaa gtaaatttta ttttaaagga gaatttgtta tgaatttaac
      121 atttttaggc ttatgtattg cctgtatggg cgtatctgtc ggtgaaggtt tattgatgaa
      181 tggactgttt aaatcagtag cacgccaacc agatatgctt tctgagtttc gtagtttgat
      241 gtttttaggt gttgccttta ttgaaggaac tttctttgta actcttgtct tctcatttat
      301 tatcaaataa atacatggaa cgagaagaaa agggaggatt ttagatggaa gaaagtatta
      361 atccaatcat ctctattggt cctgttatct tcaatctgac tatgttagcc atgactttgt
      421 tgattgtggg agttattttt gtctttattt attgggcaag ccgcaatatg accttgaaac
      481 ccaaaggaaa gcaaaatgta cttgagtatg tctatgactt tgttattgga tttacagaac
      541 ctaacattgg ttcgcgctac atgaaagatt actcactctt tttcctttgt ttattccttt
      601 tcatggtgat tgccaataac cttggcttaa tgacaaagct tcaaacgatc gatgggacta
      661 actggtggag ttcgccaacc gctaatttac agtatgactt aaccttatct tttcttgtca
      721 ttttgttgac acatatagaa agcgttcgtc gtcgtggatt taaaaaaagt ataaaatctt
      781 ttatgagtcc tgtttttgtc ataccgatga atatcttgga agaatttaca aacttcttat
      841 ctttggcttt gcggattttt gggaatatct ttgcaggaga ggtcatgacg agtttgttac
      901 ttcttctttc ccaccaagct atttattggt atccagtagc ctttggagct aatttggctt
      961 ggactgcatt ttctgtcttt atttcctgca tccaagctta tgtttttact cttttgacat
     1021 ctgtgtattt agggaataag attaatattg aagaggaata gaaaggagta actgatgcac
     1081 gtaacagtag gtgaattaat tggtaatttt attttaatca ctggctcttt tattcttttg
     1141 ctagtcttga ttaaaaaatt tgcatggtct aatattacag gcattttcga agaaagagct
     1201 gaaaaaattg cttcagatat tgacagagct gaagaagccc gtcaaaaagc agaagtattg
     1261 gctcaaaaac gcgaagatga attggctggt agccgtaaag aagctaagac aatcattgaa
     1321 aatgcaaagg aaacagctga gcaaagtaag gctaatatct tagcagatgc taaactagaa
     1381 gcaggacact taaaagaaaa agccaatcaa gaaattgctc aaaataaagt agaagcttta
     1441 cagagtgtta agggtgaggt cgcagatttg accatcagct tagctggtaa aatcatctca
     1501 caaaaccttg acagtcatgc ccataaagca ctcattgatc agtatatcga tcagctagga
     1561 gaagcttaat ggacaagaaa acagtaaagg taattgaaaa atacagcatg ccttttgtcc
     1621 aattggtact tgaaaaagga gaagaagacc gtatcttttc agacttgact caaatcaagc
     1681 aagttgttga aaaaacaggt ctgccttctt ttttaaaaca agtggcagta gacgagtcgg
     1741 ataaggaaaa aacaattgct tttttccaag attctgtgtc gcctttatta caaaacttta
     1801 tccaggttct ggcctacaat cacagagcaa atctttttta tgatgtgctt gtagattgct
     1861 tgaaccgact tgaaaaagaa acaaatcgat ttgaagtgac gattacgtct gctcatcctc
     1921 taactgatga acagaagact cgtttgctcc ctttgattga gaaaaaaatg tctctgaaag
     1981 taaggagtgt aaaagaacaa atcgatgaaa gtctcattgg tggttttgtc atttttgcca
     2041 atcacaagac aattgatgtg agtattaaac aacaacttaa agttgttaaa gaaaatttga
     2101 aatagaaagt ggtgttcttt tggcaattaa cgcacaagaa atcagcgctt taattaagca
     2161 acaaattgaa aatttcaaac ccaattttga tgtgactgaa acaggtgttg taacctatat
     2221 cggggacggt atcgcgcgtg ctcatggcct tgaaaatgtc atgagtggag agttgttgaa
     2281 ttttgaaaac ggctcttatg gtatggctca aaacttggag tcaacagacg ttggtattat
     2341 catcctaggt gactttacag atatccgtga aggcgataca atccgccgta cagggaaaat
     2401 catggaagtc cctgtaggtg aaagtctgat tggtcgtgtt gtggatccgc ttggtcgtcc
     2461 agttgacggt cttggagaaa tccacactga taaaactcgt ccagtagaag caccagctcc
     2521 tggtgttatg caacgtaagt ctgtttcaga accattgcaa actggtttga aagctattga
     2581 cgcccttgta ccgattggtc gtggtcaacg tgagttgatt atcggtgacc gtcagacagg
     2641 gaaaacaacc attgcgattg atacaatctt gaaccaaaaa gatcaagata tgatctgtat
     2701 ctacgtcgcg attggacaaa aagaatcaac agttcgtacg caagtagaaa cacttcgtca
     2761 gtacggtgcc ttggactaca caatcgttgt gacagcctct gcttcacaac catctccatt
     2821 gctcttccta gctccttata ctggggttgc tatggcggaa gaatttatgt atcaaggtaa
     2881 gcatgttttg attgtatacg atgatctatc aaaacaagcg gtagcttatc gtgaactatc
     2941 gctcttgctt cgtcgtcctc caggtcgtga agccttccca ggggatgttt tctatctcca
     3001 cagccgtttg cttgagcgct cagctaaagt ttctgatgaa cttggtggtg gatcaattac
     3061 agccctacca tttatcgaga cacaagcagg agatatctca gcctatatcg caaccaacgt
     3121 gatttctatc actgatggac aaatcttcct tggcgatggc ctcttcaatg caggtattcg
     3181 tccagccatc gatgcgggtt catctgtatc tcgtgtaggt ggttctgcac aaatcaaagc
     3241 catgaagaag gttgctggta cacttcgtat cgaccttgct tcataccgtg agttggaagc
     3301 ctttactaag tttggttctg acttggacgc agcaacacag gctaagttga accgtggacg
     3361 tcgtaccgtt gaggtcttga aacaacctgt tcacaaacca ttacctgttg agaaacaagt
     3421 aaccattctt tatgctttga cacatggttt cttggatact gttccagtag atgatattgt
     3481 tcgtttcgag gaagagttcc atgccttctt tgatgctcaa catccagaga ttttggaaac
     3541 cattcgtgat acaaaagact tgccagaaga agcagtcttg gatgctgcga ttacagagtt
     3601 tctcaatcaa tctagcttcc aataagaata gaggtgtcag atggcagtgt ctctaaatga
     3661 tattaaaaca aaaatcgcct caacaaaaaa tacgagtcaa atcactaatg ccatgcaaat
     3721 ggtatcggct gctaagttag gtcgctctga agaagctgct cgcaacttcc aagtttacgc
     3781 tcagaaagtg cgtaaacttt tgacagatat ccttcatggt aatggagctg gtgcttcaac
     3841 taatccgatg ttgattagcc gttctgtgaa gaagacaggc tatatcgtta tcacttcaga
     3901 ccgcggtttg gttggaggtt ataattcctc tattttgaaa gctgttatgg agttgaaaga
     3961 agaataccac ccagacggta aaggttttga aatgatctgt atcggtggga tgggagctga
     4021 tttctttaag gctcgcggta ttcaaccact ttatgaatta cgtggcttgg cagaccaacc
     4081 tagctttgat caagttcgta agattatttc aaaaactgtt gaaatgtacc aaaatgaact
     4141 ctttgatgag ctttatgttt gctacaacca ccatgtcaat acgctaacca gtcaaatgcg
     4201 tgtggaacaa atgcttccga ttgttgactt ggatccaaat gaagcggatg aagagtacag
     4261 cttgactttt gaattggaaa ccagccgaga agaaattctg gagcagttgt tgcctcagtt
     4321 tgcagaaagt atgatttacg gtgccattat cgatgccaag acagctgaga atgctgcggg
     4381 tatgacagcc atgcaaacag cgacagataa tgctaagaaa gtcatcaatg atttgacaat
     4441 tcagtataac cgtgccagac aggcggcgat tacacaagaa attacagaaa tcgtagcagg
     4501 tgctagtgcc ttagaatagg ctctagtcca gctcgtatga aaatgaactt aggacctagt
     4561 tgagctagga accgacagta tcttatatag aataggagaa ggagatgagt tcaggtaaaa
     4621 ttgctcaggt tatcggtccc gttgtagacg ttttgtttgc agcaggggaa aaacttcctg
     4681 agattaacaa tgcacttgtc gtctacaaaa atgacgaaag aaaaacaaaa atcgtccttg
     4741 aagtagcctt ggagttagga gatggtatgg ttcgtactat cgccatggaa tcaacagatg
     4801 ggttgactcg tggaatggaa gtattggaca caggtcgtcc aatctctgta ccagtaggta
     4861 aagaaacttt gggacgtgtc ttcaacgttt tgggagatac cattgatttg gaagctcctt
     4921 ttacagaaga cgcagagcgt cagccaattc ataaaaaagc tccaactttt gatgagttgt
     4981 ctacctcttc tgaaatcctt gaaacaggga tcaaggttat tgaccttctt gccccttacc
     5041 ttaaaggtgg taaagttgga cttttcggtg gtgccggagt tggtaaaacc gtcttaatcc
     5101 aagaattgat tcacaacatt gcccaagagc acggtggtat ttcagtattt actggtgttg
     5161 gggaacgtac tcgtgagggg aatgaccttt actgggaaat gaaagaatca ggcgttatcg
     5221 agaaaacagc catggtcttt ggtcagatga atgagccacc aggagcacgt atgcgtgttg
     5281 cccttactgg tttgacaatc gctgaatact tccgtgatgt ggaaggccaa gacgtgcttc
     5341 tctttatcga taatatcttc cgtttcactc aggctggttc agaagtatct gcccttttgg
     5401 gtcgtatgcc atcagccgtt ggttaccaac caacacttgc tacggaaatg ggtcaattgc
     5461 aagaacgtat cacatcaacc aagaagggtt ctgtaacctc tatccaggct atctatgtgc
     5521 cagcggatga ctatactgac ccagcgccag caacagcctt cgctcacttg gattcaacaa
     5581 caaacttgga acgtaaattg gtacaattgg gtatctaccc agccgttgac ccacttgctt
     5641 caagctcacg tgccttggca cctgaaatcg ttggagaaga gcactatgca gttgctgctg
     5701 aagtaaaacg tgtccttcaa cgttaccatg aattgcaaga tatcattgct atccttggta
     5761 tggatgagct ttctgatgaa gaaaagacct tggttgctcg cgcccgtcgt atccagttct
     5821 tcttgtcaca aaacttcaac gttgcggaac aatttactgg tcagccaggt tcttatgttc
     5881 cagttgctga aactgtacgt ggctttaagg aaatccttga tggtaaatac gaccacttgc
     5941 cagaagatgc cttccgtggt gtaggttcta tcgaagatgt gattgcaaaa gctgaaaaaa
     6001 tgggatttta agaggtgacc tatggctcag ttaactgtcc agatcgtgac accagatggt
     6061 ctcgtctatg atcaccatgc cagctatgta tcggttcgaa ctctggatgg tgagatgggg
     6121 atcttgccac gacatgaaaa tatgattgcg gttttagcag ttgatgaagt aaaggtaaaa
     6181 cgtatcgatg ataaagatca cgtgaactgg attgcagtaa acggtggcgt tattgaaatt
     6241 gccaatgata tgatcacaat cgtcgctgac tctgcagaac gtgctcgtga tatcgatatt
     6301 agccgtgcag aacgtgccaa acttcgtgca gaacgtgcaa ttgaagaagc acaagacaaa
     6361 catttgattg accaagaacg tcgtgctaag attgctttgc aacgtgccat taaccgtatt
     6421 aatgtcggaa atagactata agaaaaaatg aacttgaaaa taccaagttc attttt
//
LOCUS       DBEJAQ010000074         6452 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.074, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000074 DBEJAQ010000000
VERSION     DBEJAQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.074"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(33..212)
                     /locus_tag="ACL297_001937"
     CDS             complement(33..212)
                     /locus_tag="ACL297_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072136.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(354..503)
                     /locus_tag="ACL297_001938"
     CDS             complement(354..503)
                     /locus_tag="ACL297_001938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072137.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            784..1251
                     /gene="tadA"
                     /locus_tag="ACL297_001939"
     CDS             784..1251
                     /gene="tadA"
                     /locus_tag="ACL297_001939"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGR0072138.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            1284..1377
                     /gene="ffs"
                     /locus_tag="ACL297_001940"
     ncRNA           1284..1377
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACL297_001940"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1460..2176)
                     /locus_tag="ACL297_001941"
     CDS             complement(1460..2176)
                     /locus_tag="ACL297_001941"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGR0072139.1"
                     /translation="MKFAIRMNYIDTNEMLFVETPRKVITSDELRKKNTKYLDQKEFK
                     LFIQNLKDEALCDYRITKYIRIAKVLFLTGMRYGELAALNYKEDIDFSKKTIHIKHTY
                     DFRQKERTTPKTIKSDRVITAPQKVLDIIKEQIIENATNGFDTDFIFINTLGEPITNA
                     RVICALKRHGQKIGIEKNITTHTFRHSHISLLAELGIPLTAIMDRVGHSDSKTTLEIY
                     SHVTQKMVSDISSKLDKIKF"
     gene            complement(2248..2595)
                     /locus_tag="ACL297_001942"
     CDS             complement(2248..2595)
                     /locus_tag="ACL297_001942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072140.1"
                     /translation="MEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAAIL
                     LQEKIKQKLGEKQHSVSNITFEKLYEEFEENWKHGVKNSTVYASKNVKKEILKQIEGD
                     YLVRNLIDVYYKK"
     gene            complement(2656..3726)
                     /locus_tag="ACL297_001943"
     CDS             complement(2656..3726)
                     /locus_tag="ACL297_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HGR0072141.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            complement(3743..4123)
                     /locus_tag="ACL297_001944"
     CDS             complement(3743..4123)
                     /locus_tag="ACL297_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGR0072142.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            complement(4136..4399)
                     /locus_tag="ACL297_001945"
     CDS             complement(4136..4399)
                     /locus_tag="ACL297_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGR0072143.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(4399..4632)
                     /locus_tag="ACL297_001946"
     CDS             complement(4399..4632)
                     /locus_tag="ACL297_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072144.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(4632..5000)
                     /locus_tag="ACL297_001947"
     CDS             complement(4632..5000)
                     /locus_tag="ACL297_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0072145.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            5572..5763
                     /locus_tag="ACL297_001948"
     CDS             5572..5763
                     /locus_tag="ACL297_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGR0072146.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            5786..5989
                     /locus_tag="ACL297_001949"
     CDS             5786..5989
                     /locus_tag="ACL297_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072147.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(6144..6311)
                     /locus_tag="ACL297_001950"
     CDS             complement(6144..6311)
                     /locus_tag="ACL297_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HGR0072148.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            6331..6452
                     /locus_tag="ACL297_001951"
                     /pseudo
     CDS             6331..6452
                     /locus_tag="ACL297_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
BASE COUNT         1802 a         1075 c         1305 g         2270 t
ORIGIN      
        1 tgttttgtca aaaataaaaa cagtgaccga aatcactgct tatcagttgt agcaaattca
       61 taaagttttt ctgctgtgag aagtgccatt ttgtccatgc ttgtttttcc ttttctgagg
      121 tcagaaacag tagtccatgg aactccagca ccttgggaaa tagcagatgt agacatcgaa
      181 ctgtctaaca attcttgaat aacttctctc atcttatttg tcctttttat tttttagata
      241 aatatataca ttgattgcaa ttataaaaat agctattgca ctaaccattg catttcctct
      301 ttccatttga taaaatagag gtgtaagggg ctttcgcccc aacctcttag cgtttacctt
      361 tttctttgac gggatttcgg tttacgcttt ttgttttgcc ttgcgaccgt tattgcggtc
      421 actagacttg cgatagcagt taccgtttca ggaatattgt ctattgcctt ttcaagtaac
      481 ctgagccaat cttctttgtt caacttcatc accccctttc cttatcttga ttatattata
      541 tcacggtaca ccgagaaagt caagcgtttt gatgaagttt ttttaatttt ttcaaaaaaa
      601 aatagacctt gtccagaggt cggggagttg gaggggacgc cctccaagag tgttgattta
      661 ataagatttt attttacctt tttcataata atctccctat agagtcaccg cattcggtgg
      721 cttcttttgt gttgggattc atgatataat aataaaatcg ataagtagga aaagagaaaa
      781 gagatgtatt atacgcttga agaaaaagaa gtctttatga gggaggcttt gagagaggct
      841 gagattgctc ttgaacacga tgaaattcca attggttgtg tgattgtcaa agatggggaa
      901 atcattggtc gtgggcataa tgcgcgtgag gaattacagc gagcggttat gcatgcggaa
      961 attatggcta tagaggatgc gaacttgagt gaggagagct ggcgcttgct ggattgcaca
     1021 ctttttgtga ccattgaacc ttgtgtcatg tgtagtgggg cgattgggct tgcccgcatt
     1081 ccaaatgtgg tctatggggc taaaaaccag aaatttggcg ctgctgggag tttgtacgat
     1141 atcttgacag atgagcgtct caatcatcgt gtggaggttg aaataggaat tttggaagat
     1201 gaatgcgcag ctatcatgca ggattttttt agaaatagac ggaaaaaata attttgcttt
     1261 taaaatgaat aggaatgtga tataataaat agtggagcaa cagttctgcg tgaagcgggt
     1321 caggggagga atccagcagc cctaagcgat ttgaattgtg tgctcttttt tcgtgcactt
     1381 taaaaaccct ttaaaatcaa cactttaagg ggtttttgtt tgtcttgtat aagaaaaagg
     1441 ggcagacgag gggcacaatt taaaatttta tcttgtctaa cttgctagat atgtctgata
     1501 ccattttttg agtaacgtga gaataaatct ctagtgtggt ctttgagtca ctatgcccta
     1561 ctctgtccat gatggcagtc aagggaatgc ccagctcagc aagtagggat atgtgagaat
     1621 gtctaaatgt atgtgtagtt atgttttttt ctatgccgat tttttgacca tgtcttttca
     1681 atgcacaaat aaccctggca tttgttattg gttctcctag agtattgatg aaaataaaat
     1741 ctgtatcaaa tccatttgtc gcattctcta ttatttgctc tttgataata tctaacactt
     1801 tttgaggtgc tgttataacc ctatcggact tgattgtctt tggtgtagtt ctctcttttt
     1861 gtctgaaatc gtatgtatgc ttgatgtgaa tagtcttttt agaaaaatct atatcttcct
     1921 tgtagtttaa ggctgccagc tctccatacc tcatgccagt aagaaaaaga actttagcta
     1981 ttcggatata ctttgtaatt cgataatcac atagggcctc gtcttttaaa ttttggatga
     2041 ataacttaaa ctctttttgg tctaagtatt ttgtattttt ctttctgagt tcgtcggatg
     2101 taattacttt tctaggcgtt tcaacaaata gcatttcatt tgtatcaata taattcattc
     2161 tgatagcaaa tttcattatc tgattgagct tgaatttgat tttagaaaca tagttatgag
     2221 atctcccatc ttgtaatagt tgatctatta ctttttgtaa taaacgtcta tcaaatttct
     2281 aactaggtag tcgccctcta tctgctttaa aatctctttt tttacattct ttgaagcgta
     2341 gacggttgag ttttttacgc cgtgtttcca attctcctca aattcttcat atagtttttc
     2401 aaaagttata ttagaaacag aatgttgttt ttctcctaac ttttgtttta tcttttcctg
     2461 aagtaaaatc gcagcttgat ttcttgcttg gggagttttt ttctccatcg ttactgagac
     2521 tttttttaat ttctcagtat atggatcttt gtatctctca aaaaatttat atttgccgtt
     2581 tggcaattct tccatccaca ttgattttac ctcacttttt tgataaaatg ggtatagtaa
     2641 agagggcttt ttttattatg agaatttctt gattacttct aaaatttcat tggcataatt
     2701 tgcgacttcg agaggggtag acaccgggaa gatgccttta tcacggattt cgatagtgct
     2761 tttttttgaa tttgaacgat aacgtaatac ccattttttg atgttatcgt caaccaaaac
     2821 attgaaatag ctacggttat ctctgtaaaa tacacgttct ggagaaacta catccctagc
     2881 aagcatttta acaacagtat aaacttctaa ttcagcagga gttgtgataa tttcgtcagt
     2941 tacttcgaca atctcttcag cttcagcttc aacttttgga atatcggttg ttactttggt
     3001 ttcaacgctt gtattaagtg cagcacttaa tttttcatta actcgctctg tgatgaattg
     3061 attaaaccct ttcacaataa tgggagaaaa tgtcgttaag atattttgag taacacgccc
     3121 ttcatagatt tcagaagtta gatatctgag gaagctatct gacggtgtag tgatattttc
     3181 agtaaggaac gctttaagat tgttgaggta tttcaattca gaagctgatg aaacaatatt
     3241 atcaatatca aaattctctt tgtggaattt gatgatttca gtaaattgat tttctttgat
     3301 atcagtcaca tcgattgtta agaatggagt tgtgtccatt ttatttggct catctaaatc
     3361 agtaaagaat ttatattctc taccatttgt caagataccg aattttgatt tagtagttac
     3421 gaaatatctg aatagttgag agtcgtgctt agtaaggttt tctgtaattg atttacattc
     3481 aattaggatt tgaggctcgc catccaaaat gatcgcatag tcgacttttt cgcctttttt
     3541 aatgcccacg tcggcagtaa attccgggac aaattcaagt ggattgaaga tatcatatcc
     3601 aagcgcttgg aagaacggca taatgaaggc attctttgtt tgttcttcat tcgtaatgct
     3661 ttggctgagg tctgctacac gtttgccgac ttgttttaaa tcggctttta ctttatcaat
     3721 ttccatattg atactccttt ttttaattta ctaatgctaa atactcttct ttaaccatga
     3781 tttcatttgt catggttttt agattgtagt aagacatgaa tttgaggtaa tcaaactctg
     3841 tggggtcgtc taagttttct agagcgtctt ttacgagatg atggatcata ttcctatcag
     3901 cttcgttttc acagcgtaga cgggcgttct ggtgctctga gcgtgtgtgg tctttgtgtc
     3961 caagttcgtg tagggcgact tggatttgtt gttcaggagt caagttgtgg tcaatagcga
     4021 gcacgtttgt gtctggattg tagaaaccgc tattgtgcca gtttgaaccg tcgaagagac
     4081 aaagttctac ttgatattct tcgcagagtt tagcgagtgt catagttctc cttcgttatt
     4141 tatagacatc tctgcggtgg gcgatttcca cggctaggac gactagttta tcgtcttgga
     4201 tatcacagat gatgcggtaa ttctctactc tgtatcgcca gtaacctgca aggttggctt
     4261 ttagtgcttt tccgtgttgt cgtggattgg tcgtgttttc aatgttttta gcaagccagg
     4321 ataggatttg ttttctggtt ggggtatcta gttttttaag ttgcttgaga gcttttttat
     4381 caatatctag ccgatacatt aagcaatatc ctctcgagtt agtcctagtt catccaagac
     4441 ctcatccatg gtataagtag ctgggtcggc taaatactcc atataggctt ggtcggctgc
     4501 tcgtgcgtct tcgatatctt ccatgagtgc catgaagtcg tcaaaatcca tggtcgttgt
     4561 gtcgataccg tgtttgttta ggtagtccgt aatgtaggaa tttttttctg tgaagttgat
     4621 agtgatagtc attagcgttc tcctttgctt ttgaagtggg cggataggac ggatgtaatg
     4681 aagtcgatat catcttcatt tagtggtttc ccgtcaaata acatggtgtt ggctgctgct
     4741 ttgcgtaagt ctatgatttg tccgtttact tgggcaaatt catcactccc agcgatagca
     4801 gggttatctg ttcgtccgag caggtagtcg gtggatacgt tgaaataatt agcaatttct
     4861 gcgatacgtt cagcgtttgg cgtagaattt tttatcttat agagtgtatt tctaccataa
     4921 cctaagtctt cttctacttg tccaagagct tttccacgct tttttgccaa ttcttttatt
     4981 ttttcaaatg tctcaaacat tgttaaatca acctttctaa gacatcacaa aaaaaattta
     5041 acaaatttgg tgtaaaaagg ttgactaatt atcccaaaag gtgtaaaatg ttttttgtaa
     5101 gtgataaaca actaaaaaaa caactaagaa ataaattata aaaatgtttt ggcgaacggt
     5161 atttatagat ttattggtgt ttttattatg ctttaatttt agccgatttg gtgtaagttg
     5221 tcaagtataa tgcagaaaaa tagttacatt tttagttgtt tcttatttac aaataagtaa
     5281 agaggaggaa cgtgctgatg gtaagtattc ttaaaaattt agaacaagaa aaagaccacc
     5341 ttgaaaaagt cattaaggta gtcagcgctg gtggtaaatt tctgagattg ccatatcaaa
     5401 aaagtcacgc tcgattagtg agaatctgaa attgatttct caaaatcttt accatcatcg
     5461 tagatgtcgc gtttgcttgc tttgagtaaa tattctttaa aagtcataag gttaactcct
     5521 ttctgcttat attatagcag aaaaagaaga aagaaaaaga aaggagaaaa tatgccagat
     5581 atcgcaaacg gtcgcgaaag agttattgct ttcttgaaag agaaaggcat taaaaaagca
     5641 actctagcgg ttgcttacgg ctttaaacga caggaagtga caaacattct aagtggaacg
     5701 actaaaggtc cacgagcgaa cagtttcatt cttcaggtta ttgaagatta tgggattgag
     5761 taggaaagat ttgaggagta ggaaaatgag accaaaacga tatccgtata gtggacaaaa
     5821 agagtccacc tttgtaaagg cagaccctga gttagttgaa aaacttttaa gaaacactag
     5881 ttttcttgag tgtttacaaa aaaagcctat caattttcag atagactcag aagaatttaa
     5941 gcgtcttagc tatgaagcca ttcatgatac ttctcaagta actcaatagg aggaaggaat
     6001 aaaatgattc accattatat aactcactat gccagcaatg ggaaagatta cgccgaagca
     6061 tggattcaaa ttgatttttt gggaatgtgc ttttgtgtat ggaaaaagcg tacaaccatt
     6121 gaacgattgt acgcaaacga agattagact tttttccaac cgttgccttt agcagatgtc
     6181 ggagggagcc gatcaccttt tccgatagtt gcggtatgac cattagtaac ttttccgcca
     6241 cgaggtccta cctctacata gcgaccaggt ttctgattat ctgttccagg ttttattgga
     6301 gtatttgcca tactatctcc tcctttctat aagtttgaga aaatcaacgg tatctggtat
     6361 tatttcgatg gctcaggcta tatgctttca gaccgctgga agaagcacac agacggtaat
     6421 tggtactact ttgaccaatc aggcgaaatg gc
//
LOCUS       DBEJAQ010000075         6329 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.075, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000075 DBEJAQ010000000
VERSION     DBEJAQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.075"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(14..223)
                     /locus_tag="ACL297_001952"
                     /pseudo
     CDS             complement(14..223)
                     /locus_tag="ACL297_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            432..2201
                     /locus_tag="ACL297_001953"
     CDS             432..2201
                     /locus_tag="ACL297_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HGR0072149.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            2225..4180
                     /locus_tag="ACL297_001954"
     CDS             2225..4180
                     /locus_tag="ACL297_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGR0072150.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEIMKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            4182..4619
                     /locus_tag="ACL297_001955"
     CDS             4182..4619
                     /locus_tag="ACL297_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGR0072151.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4681..5817
                     /locus_tag="ACL297_001956"
     CDS             4681..5817
                     /locus_tag="ACL297_001956"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGR0072152.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNDFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPTQSHEDSLFVMVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1943 a         1068 c         1263 g         2055 t
ORIGIN      
        1 agccttcatg tcagacttta ttactcattt cttgttttaa atcaggagaa tagtaacgat
       61 tttttccttt tttgacgaac tctattccgt aacgatcaat caatttaatc atgtacctaa
      121 tattagaatt gcttatccca aatttatttg aaagcttctc taagctatat ccttgttttc
      181 taagttcata gatctgaact ttatcatcat aagttagttt cataataaaa acaccccaaa
      241 agttagattt tttctgtcta acttttgggg tgtagttcat gtacacctga tatgatgcgt
      301 tttataattt ttaagccttt ttgcccagcc tcgtcaaaag taatgttttg acacaaaatc
      361 tgtgacaaaa ctttagtttt aaaggttttt aactttgtat atactagttt taagaaaagg
      421 aggatgatct aatggaagaa aaagtatcat tgaaagtcag ggttcaaaaa ctagggacat
      481 cgctttcaaa tatggttatg cccaatattg gagcatttat tgcttgggga gtattgactg
      541 ccctctttat cgctgatggc tatctgccaa atgaacagtt agctactgtt gttggtccta
      601 tgttaacgta tttattgcca atcctgattg gttacacagg tggatatatg atccatggcc
      661 aacgtggtgc cgttgtagga gctattgcta ctgttggtgc aatcacaggt tctagtgttc
      721 ctatgtttat cggagctatg gtaatgggcc cactgggagg atggactatc aagaaatttg
      781 atgagaagtt ccaagaaaaa attcgtcccg gatttgaaat gttagttaat aacttctcag
      841 ctggtctcgt tggttttgca ttattgcttt tggctttcta cgcaatcggt ccagtcgtat
      901 cgactcttac tggagctgtt gggaatggtg ttgaggctat tgtcaatgct cgcctccttc
      961 ctatggctaa tattatcatc gaaccggcta aagtcctttt cctcaataat gccctcaatc
     1021 atggcatttt tactcctctg ggagtagaac aggtagctca agctggtaag tcaattctct
     1081 tcctattgga agctaatcct ggaccaggcc tgggaattct attagcttat gctgtattcg
     1141 gtaaaggttc tgctaaatct tcttcttggg gggcaatggt tattcatttc ttcggaggga
     1201 ttcatgaaat ttactttcct tatgttatga tgaagcctac tctattttta gctgctatgg
     1261 caggaggtat ctctggaact tttacttttc aactcttaga cgctggtctt aaatctccag
     1321 cttcaccagg ttctattatt gcgattatgg ctacggcgcc aaaaggtgtt tggccccatc
     1381 taaatgttct tctaggtgtt ttagtagcag cagttgtttc tttccttgta gcagccctta
     1441 ttcttcatgc agacaagtca actgaggatt cgctcgaagc tgctcaggcg gctacccaag
     1501 cagctaaggc tcagtctaaa ggtcagttag tatcaacttc tgttgatgca gttgtttcga
     1561 cagactcagt ggaaaaaatc attttcgcct gcgatgctgg tatgggaagc tctgctatgg
     1621 gagctagtat tcttcgagat aaggttaaaa aagcaggtct agagattcca gtatctaatc
     1681 aggcaatctc aaatttgctt gatacaccaa aaacattaat tgttactcag gaagaactga
     1741 caccaagagc taaagacaag agtccaagtg ctattcatgt ttctgttgat aatttcttag
     1801 cgtcccctcg ttatgatgaa attgtagctt cattaacagg agcttctcca atagcagaaa
     1861 ttgaaggaga tataccaact tcagcaccag tagatagtca ggaaattgac cttaaccata
     1921 ttgatgctgt agtagttgct tatggtaaag cacagggaac tgcaactatg ggctgtgaaa
     1981 cgattcgggc tatctttaga aacaagaata ttcgtattcc agtttctact gccaaaattt
     2041 cagaattagg tgaatttaat tctaaaaaca taatgattgt aacaactatt tctttacagg
     2101 cagaagtgca gcaagcagca ccgaattctc aatttcttat tgtggatagt ttagtaacaa
     2161 caccagaata tgacaaaatg gctgctagaa tgtacaaata gaactagagg tttctaaatt
     2221 acgaatgcta ttaaccaaac gagaagaaca attattgaag gctttcctac atgtagggaa
     2281 gctttcaatg caagatatga ctgaaatctt acaggtttca tctagaacaa tttatcgaac
     2341 tttatcagat ttgacagata gcatggagca atatggaatc gaaataatga agcatgggaa
     2401 atactatatt ttgactggag agttggatga tttgccgaca gaacttgaag tgttagttga
     2461 gtatagtccc caagaaagac aagagttgat tacctatcgc cttctgactg agagcggttt
     2521 tgtcaccaat gaagcattgc aagagtgcac gaaagtcagt aatgtaacta ttattcagga
     2581 tatttcagat attgataagc gtcttttaga ctttgatctg aaaattgaac gacaaaaagg
     2641 ttatcggatt tctggtgatt cagttggtaa gagaagattt ttggctattt tactgacaaa
     2701 ctgtatctca gtagcagatt tttcaaccgg taattttggg agctttgata ttttagaagc
     2761 agatagaact aggctggcca gtcagattgt taataagcaa ctgtcaggtt ttccagatat
     2821 ggatgctagg atgaagatgt tttttgcgat cttgttatct cttataggtc aggagcaaaa
     2881 cattgaaaat tcacctaata ctagtaagca ggctttggaa atttctcaaa aaatttttca
     2941 aacttactct aagcagactg cacaatttta tagtattcag gaaattatct attttgcgag
     3001 catcttggat gaattaatca ttaaacgtca ggacaatccg ctctttacgg agaaatttga
     3061 tggtgaattt ttctacaata tttcaaatct gattgatacg gtttccatgt ataccaagat
     3121 tgactttttt aaggacaagg ttttattcaa ttttcttttc catcatattc ggctcagttt
     3181 aggcgtccct atcctttttc agggtgaaaa tttgccagaa tctgtccaga ttttagttga
     3241 aaggaataaa tttctttata cagtcatcag tcttttagtg aatgatattt ttccgaaata
     3301 tcttcataca gagtatgagt atggcatgat tgccctacat tttatctcta gcttaggccg
     3361 tagtccagag atttatccag tccgtgtttt gcttttaacg gatgaacgtc gggtcactag
     3421 agatttatta gtcagtaaaa ttaagagtgt tgctcctttt gtagagttga tagatattca
     3481 gtctctagta gattaccaca gtattgatct cagtcagtat gattatattt tatctaccaa
     3541 gccgctgact aatcaggaaa tcgatgtaat ttctagtttt ccaaccgtca aagaattgct
     3601 tgaattacag gaacgacttc agtatgtaca ggcacatcgt acaattgtcg cgcgtgatgc
     3661 tatcgctcca gagaaaagtt atgacttgca agattattta atatctagta gtcagctttt
     3721 gagtcaattc gagttggttc aattggagaa taatcaatca tttgagcaca cggtagaaca
     3781 aatcatccaa tatcagaaga atgtgagtga cagagattac ctaacaagaa aattgttatc
     3841 tcacttccag aatagtccta tggctattcc taatactggt ctggtgcttt tacatagtca
     3901 gtctagcaaa gtaacaacaa atagttttac tatgtttgaa ctcaaactac ctatctccgc
     3961 attgtcaatg aaacgagagg aagaagaggt caaaaggtgt ctgctaatgc taatgtctaa
     4021 agaagctagc gaggaagcga gagatttaat gacagctatc agtcagtcga ttattgaaaa
     4081 tcatctttat acagagattt acaagacggg aaatcaatcc attatttatc agatgctaaa
     4141 tactattttt aacgaaaaaa ttaagaaatt ggagaactaa tatgaaactt gaaaaacatt
     4201 tgattaagct taataaacaa ttttctaaca aggaggaagc tatttgttat tgtgggcaag
     4261 ttctttatga gggtggatat gttaatgaag actatattga agccatgatt gagcgagata
     4321 aagagctatc tgtttacatg ggtaacttta tcgccatacc gcatggaaca gatgcagcaa
     4381 aaaatgatgt cctcaagtct ggtattacag tcgttcaagt ccctagaggg gttgattttg
     4441 ggaatgtatc taaccctcaa gtggcaacgg ttctttttgg tattgctggt attggtaatg
     4501 aacacttaga aattattcag aaaatttcta tcttctgtgc agatgtagat aatgttctta
     4561 aactagcaga tgctcagtca aaagaggaag tattgcgctt atttgatgct gttgaataat
     4621 tgaatttcgt catttgtcat ctagtatata tgtccctcaa ataggaaaag gagaaattga
     4681 atgaaacatt ctgttcattt tggtgccggt aatatcggtc gtggttttat aggtgaaatt
     4741 ctatttaaaa atgatttcca tattgatttt gtggatgtca ataatcagat aattcatgct
     4801 ctaaatgaaa agggcaagta tgaaattgaa attgcacaga aaggacagtc tcgtatagaa
     4861 gtaactaatg tggctggaat taatagcaaa gaacatcctg agcaagtcat tgaagcgatt
     4921 caaaagacgg atattattac tactgcaatc ggacctaata tactcccttt tatcgctgaa
     4981 cttctagcca aaggaatcga agctcgccga gttgcaggaa atacacaggc attggatgtt
     5041 atggcctgtg aaaatatgat tggcgggtct caatttcttt atcaagaagt caagaaatat
     5101 ttaagtccgg aaggtttgac atttgctgat aactacatag gttttccaaa tgctgcagta
     5161 gacaggattg ttccaacaca aagtcacgaa gattcccttt ttgttatggt cgagcccttt
     5221 aatgaatggg tcgtggaaac caagcgtctt aaaaatccag atttacgtct agaagatgtg
     5281 cattatgaag aagatttaga accctttatt gagcgaaaac ttttttcagt caattctgga
     5341 catgcaactt cagcttacat tggtgcgcat tatggtgcca agacaatttt ggaagctctt
     5401 caaaatccta atatcaaatc tcggattgaa tctgtattag ctgaaattcg gagtctcttg
     5461 attgccaaat ggaactttga taaaaaagaa ttggagaatt atcacaaagt cattatagaa
     5521 cgattcgaaa accctttcat agtggacgag gttagtcgcg tagctcgtac tccaatccga
     5581 aaattaggct ataatgaacg attcatccgg ccgatacgtg aattgaaaga actcagtttg
     5641 tcatataaaa acctacttaa aacagttggc tatgcctttg actatcgcga tgtaaatgat
     5701 gaagaaagta ttcgattagg tgaattgttg gctaaacaat cagtcaaaga tgttgttata
     5761 caagttacag gtttagacga ccaagaattg attgagcaaa ttgtagagta tatttaatct
     5821 ttttcgaaaa tctcttcaaa tcaggttagc atccatctgc aacctcaaaa cagtattttg
     5881 agctgacttc gtcagttcta tctacaacct caaagcagtg ctttgagctg actccgtcag
     5941 tcttatctgc aacctcaaaa cactgtttga gttatctgcg gtaatctttc tagcttgtct
     6001 ttgatttttg ttgttattta taaggtaaaa gaagctggac aaaaagtctt caaaatcgga
     6061 aaaaggcagc ctatcgggtg ttcaaaaatc ttgataggat gtcctttatt atggaaagtc
     6121 ttattggatt ttctcctcag attgagtttt tgatcagctt tatgagatag gttttgctag
     6181 agatgtagcc catcatgtta tttttatgga cagtgggaaa attgttgaaa aaaataatgc
     6241 ccatcaattc tttagtcgtc caagagaaga acgaaccaag caattttgga acgaattctt
     6301 tcgaatgtga tctatatagt aaaatgaaa
//
LOCUS       DBEJAQ010000076         6270 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.076, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000076 DBEJAQ010000000
VERSION     DBEJAQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.076"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..4211
                     /locus_tag="ACL297_001957"
     CDS             <1..4211
                     /locus_tag="ACL297_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HGR0072153.1"
                     /translation="WIWMPKFEISDVDTSYSEAPEDIEGQISTVESNFKQRADSLEAG
                     VSRLTEGLRTKADISALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQE
                     ILNATKNKASKSELTQTAEELSSKIASVQVGGINLLRNTASLLIGDRSKGCWMSASGG
                     NGRAISVEVLDPPKKMIKNMIRVIENTNGGNKDLTQLVRLRIGEKYTISCYARIASDS
                     PNANVNLLFRSWANNTDLNRKFQKSISHKNWQKYSFTFTADAIENSIQFGQSGAGIIE
                     ICAPKIESGTLATDYSEAPEDIEGHISTVESTFKQRANSLDAGVSRLTEGLRTKADIS
                     ALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRASGIRQEILNATKDKADKTLVT
                     AEAGKLREELTSLSVGENLFVNSDFKNLRDNGQRYTANGNTYQNMIAPYWYNPYNAGI
                     PNAQNIQHGYFDTETFSDTVFAFNESDGSRHWKALSTDFKIGVISAGEYYFSADLYAT
                     DLGTHIKFGFYYHNSTGKLNFYAGRTKIEVTEKGRWTRLGIDLKVNDDIDLTKKVQFY
                     IYGYNFARNSILYLKKPKVSKGRLKSDWSPALEDTEGLITEAKATFERTAQGLRTDLS
                     AIQEYVNKDGQRQEALQRYTREESTRQATAVRELVNRDFVGKATYQEDVKGINQRIEA
                     VKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGGQISGLSNRLTSSEQGTTT
                     QISNLSNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRISDQVSANKANADSQFAN
                     VTNQLVRKVETTDFQRVKETSKLYERILGNTENGIADKVARMALTNQLFQVEVGKYSV
                     SGPNLIKNSDFKNATNEWGSTQNLGRLVKHSFYHNGQKDLMRLSNATKNENFLYSHRF
                     NLERNTDYVLNFRGFNNSALASYDVYILGRRAGESDGFTIVKKVVSSKKLSTSRCEDV
                     SVTFNSGEMDNAYIRFDNNGSSSGTADLYITEVDLYKGYKPRTWQPHPEDAVADANKK
                     LEATQTKMTQLAGSWVVENINSAGDIISGINLGANGHNRLVGKLTHITGETLIDRAVI
                     KSAMVDKLKTANFEAGSVTTTILEAEAVTAEKLKVDDALIRKLTAKDAFIDRLTSKRI
                     FSTKVESVISSSTFLEAYQGRIGGFTLGQFDQGGGRWISGVNQFSVGMGNGAGYGVRT
                     AFWANWGNNWNYAGPKAWNVNTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTTFSRSP
                     VFSNGIELGSKDVLGDGWNPKGGRNAVVWWNQVGSGSLKYWMEQKSDRRLKENITDTA
                     VKALDKINRLRMVAFDFIENKKHEEIGLIAQEAETIVPRIVSRDPENPDGYLHIDYTA
                     LVPYLIKAIQELNQKIEKMEKTIA"
     gene            4208..4324
                     /locus_tag="ACL297_001958"
     CDS             4208..4324
                     /locus_tag="ACL297_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HGR0072154.1"
                     /translation="MNNNMLTNIALKAIQDLALENRKRAHRLENLENEHRTA"
     gene            4305..4508
                     /locus_tag="ACL297_001959"
     CDS             4305..4508
                     /locus_tag="ACL297_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072155.1"
                     /translation="MNTEQLNQALRMTISEISTTSTNSMIESNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            4511..4861
                     /locus_tag="ACL297_001960"
     CDS             4511..4861
                     /locus_tag="ACL297_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072156.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            4870..5286
                     /locus_tag="ACL297_001961"
     CDS             4870..5286
                     /locus_tag="ACL297_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGR0072157.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFVFLHSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            5290..5622
                     /locus_tag="ACL297_001962"
     CDS             5290..5622
                     /locus_tag="ACL297_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGR0072158.1"
                     /translation="MQQITEIIIAFATSFLTVAVGGIVKAVKDYLLRKGGEKAVIIAE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            5626..>6270
                     /locus_tag="ACL297_001963"
     CDS             5626..>6270
                     /locus_tag="ACL297_001963"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGR0072159.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLMIETGWQKNNTGYWYVHSDGSYPKDKFEKVNDTWYYFDSSGYM"
BASE COUNT         2058 a         1163 c         1453 g         1596 t
ORIGIN      
        1 tttggatttg gatgccgaag tttgaaataa gcgatgtaga tacttcttat tcagaagctc
       61 ctgaagatat agaaggtcag atttcaacag ttgaatccaa ctttaaacag cgtgctgatt
      121 cactcgaagc tggtgtaagc cgtctgactg aagggcttag aactaaagcc gatatcagcg
      181 cactcaacgt gactgctgag aatattaggc aatctgtgaa gagtcttgag acagacacgc
      241 agaacaagct aaatcagaag ttgagtcagg ctgaatttga ggtgcgagct ggctctatcc
      301 gtcaggaaat cctgaacgca accaagaata aagccagcaa gtcagaactc acgcagacag
      361 ctgaggagct ctctagtaag atagcgagtg tgcaggtcgg gggaatcaac ctcttgcgta
      421 atactgcgag tttgttgatt ggcgatcgtt caaaaggatg ttggatgagt gcaagcggag
      481 gaaatgggcg agcgattagc gtagaagttt tggatcctcc caaaaaaatg ataaaaaaca
      541 tgattcgtgt tattgaaaat acgaatggtg gaaataaaga tttaactcaa cttgttagat
      601 tgcgaattgg tgaaaagtac acgatttctt gttatgcaag gattgctagc gatagcccaa
      661 atgcaaacgt gaacttgcta tttcgttcgt gggcaaataa taccgattta aatcgcaaat
      721 ttcagaaatc catctctcat aaaaattggc aaaaatattc atttacattt actgctgatg
      781 caattgaaaa ttcaattcaa tttggacaat ctggcgcagg aattatcgaa atatgcgctc
      841 cgaaaatcga atcaggaacg ttagcgactg attacagcga agctcctgaa gatatagaag
      901 gtcatatttc aacagttgaa tcgaccttca aacaacgagc caactcgctc gacgctggtg
      961 taagccgtct gactgaaggc cttagaacca aagccgatat cagcgcactc aacgtgactg
     1021 ctgaaaatat ccggcaatct gtgaagagcc ttgagacaga cacgcagaac aagctaaatc
     1081 agaagttgag tcaggctgaa tttgaggtgc gagcaagcgg aatccgtcag gaaatcctga
     1141 atgcaaccaa ggacaaggct gacaagacct tggtaacagc ggaggctggg aagttgagag
     1201 aggagctgac aagtctgtcg gttggcgaga atctgtttgt aaactcagat tttaaaaatc
     1261 tgagggataa cggacagcgt tatacagcta atggcaatac ctatcaaaac atgattgcgc
     1321 cttactggta caatccgtac aatgctggta ttccaaatgc tcaaaatatc cagcatgggt
     1381 attttgacac ggaaacgttt agtgatactg tctttgcctt taacgagagc gacggctccc
     1441 gacactggaa agcattgtca actgatttta aaatcggagt catctcagct ggagagtatt
     1501 acttttcagc tgatttgtat gcgacagatt taggcactca tatcaaattt ggattttact
     1561 atcacaactc gactggcaaa ctcaattttt acgcaggtcg aacaaaaatt gaagttactg
     1621 aaaaaggccg ttggactagg ctaggaattg acttaaaggt caatgatgac attgacctga
     1681 caaaaaaagt ccaattttac atttacggct ataattttgc tagaaactca attttgtacc
     1741 ttaaaaagcc taaagtctcg aaaggtcgct taaaaagcga ttggagccca gctctggaag
     1801 acaccgaagg cctcatcact gaggctaagg caacctttga gcggacagct cagggcttgc
     1861 gaaccgactt atcagctatt caggaatatg taaataaaga cggtcagcga caggaagccc
     1921 tacagcgcta tactcgtgag gagagcacga gacaagcgac agcagtccgt gagctggtca
     1981 atcgtgattt cgttggtaag gctacttatc aagaagatgt taagggtatc aatcagagga
     2041 ttgaagctgt taaaactagt gcgaataaag acatcgctag tcaaatcgct agctatcgcc
     2101 aatctgtaga tggtaagttc acggatattt caagtcagat aactacttat aagcaagatg
     2161 tgggcggtca aatcagtggt ctatcaaata gacttacaag cagtgagcaa ggaaccacta
     2221 ctcagatttc aaatctttca aatcggataa acagtaataa gcaaggcaca gataatcaga
     2281 tttcaaattt aaagactcag gtcgctacaa acaaggataa tgctgaacga caaatgggta
     2341 gaatatctga tcaggtttct gcaaacaaag cgaatgctga tagtcaattt gcgaatgtga
     2401 ccaatcaact agtgcgaaaa gtagagacta ctgacttcca gcgtgttaag gaaaccagta
     2461 aactttacga gcggattttg ggcaatactg aaaatggaat tgcggataag gttgctcgca
     2521 tggctctgac caatcaacta tttcaggttg aggttgggaa atatagtgta agcggcccta
     2581 acctcattaa gaatagtgat tttaaaaatg ctacgaatga atggggctca actcaaaatt
     2641 taggaagatt ggttaagcat agcttttatc acaacgggca gaaagacctt atgcgtttaa
     2701 gtaatgcaac taaaaacgaa aactttttgt atagtcaccg ttttaatctt gaacgaaata
     2761 ctgactatgt actgaatttt agaggattta acaacagtgc tctcgcaagc tatgatgttt
     2821 atattttggg acgaagagca ggcgagagcg atggattcac aatcgttaag aaagttgtta
     2881 gcagcaagaa actatctacc tctagatgcg aagatgtctc agtaactttt aattccggag
     2941 aaatggataa tgcttacatt cgttttgata acaatggctc atcatcagga acagctgatt
     3001 tgtatattac agaagttgac ttgtacaaag gttataaacc tagaacatgg caaccacatc
     3061 cagaagatgc agtcgcagat gcgaataaga agcttgaagc aacgcaaaca aaaatgactc
     3121 aactagctgg ctcatgggta gttgaaaaca tcaactcggc tggagatatc atctctggaa
     3181 tcaatcttgg cgccaatgga cataaccgct tagttgggaa attgacccac atcactggag
     3241 agaccctgat tgacagagca gtcatcaagt ctgccatggt tgataagctc aaaacggcca
     3301 attttgaagc tggttcggtc acgactacga tattagaagc tgaagcggta actgctgaga
     3361 agttgaaagt tgacgatgcg cttattagaa aattaactgc aaaagatgct tttattgacc
     3421 gactgacatc taaacgtatc ttctctacta aggttgagtc cgtcatttct agctcaacct
     3481 tcctagaagc ctatcaaggc cgaatcggtg gattcacact tggtcaattt gaccagggtg
     3541 gcggtcgctg gatttcaggt gtcaatcagt tctctgttgg tatggggaat ggtgccgggt
     3601 atggagtccg gacagccttc tgggcgaact ggggaaataa ttggaactat gccggaccta
     3661 aagcatggaa cgtcaatact gatgggaaaa tgtactgtag gaatgaagtc ggtttttatg
     3721 atcaagtgga tttttcgaat tcatcgagag caaacttcta tgggaatact actttttctc
     3781 gttctcctgt gttttcaaat ggtatcgaac ttggaagtaa agatgtgctt ggtgatggtt
     3841 ggaatcccaa aggcggaagg aatgcggttg tttggtggaa tcaggtcggt agcggtagct
     3901 tgaagtattg gatggaacaa aaatcagata gacgcttaaa agagaacatc acagatacag
     3961 ctgtgaaagc cttggataaa atcaacagat taagaatggt tgcatttgat ttcatcgaaa
     4021 ataagaaaca tgaggagatt ggtctaatag ctcaagaggc tgaaaccatc gttccaagaa
     4081 ttgtctcacg agatcctgag aatccagatg gctatctaca tatcgactat accgctttag
     4141 ttccttactt aatcaaggct attcaagaat taaatcaaaa aatagaaaaa atggagaaaa
     4201 caatagcatg aataacaaca tgttgaccaa tatcgcactt aaagcaattc aggatcttgc
     4261 tcttgaaaat agaaaacgag cacacagatt ggagaactta gaaaatgaac acagaacagc
     4321 ttaaccaagc cttacgaatg acaattagtg aaatatcaac aacttcaaca aattcgatga
     4381 ttgaaagtaa tatcttgagt attcagttgg atgagcaaag ggaagagaat caaagacttc
     4441 aagcacgagt ggatgagctg gaagctctgc ttgatgaaca aactaaacca gcagacaaag
     4501 gagaatagac atggcagaaa caattcaaaa cacagataac ttactagacc ttacaaaaat
     4561 cacagaacca tttgatcttg cgagtgcttt gcgctacatg aaagaaaatg gagagttcat
     4621 tcgttgcaag aatgtaagcg atgacttcta tatgtatcgt gacgttcaaa aacgtcctgt
     4681 gatcgtaaat ggccgtcgcc aattcaagga tgttgaaacc gtttgggcgt tcaatcagtg
     4741 gggtggtaca atcgcaacaa tcaacgtagc cgttctgttg aatcatgaat tctatatcat
     4801 gaaatttgat gcagagggca atcctgactg gacggttcca acggtagaac ctaaagaata
     4861 ggaggttgta tgcaaattga atttttcaat tttctaagaa gtgtcgtaca gactgaagat
     4921 ggtttggttt tgtacgctct agcactgatt gtctcaatgg aaatcattga ttttgtgaca
     4981 gggacgattg cggcgattat caatcctgac atcgagtaca agagcaaaat cggcattaac
     5041 gggctccttc gtaagatttc aggggttctc ttactgatga tcctcattcc ggcgtccgtt
     5101 ttgttgcctg aaaagacagg ttttgtattc ttgcactcaa tctatctcgg gtacatcgca
     5161 tttacttttc aatctctcat tgaaaattac cgcaaattaa aaggaaatgt tactcttttt
     5221 cagccgattg taaaagtatt tcagcgatta cttgaaaaag atgatgacac gaaaaaagga
     5281 gaataacaaa tgcagcaaat tactgaaatc atcattgctt ttgcgacaag ctttttaaca
     5341 gtagcagtag gcggtattgt aaaagcagta aaagattatc ttttgcgtaa aggcggagag
     5401 aaagcggtga tcatcgctga aattctagct aaaaatgcag taaatgccgt ggagcaggta
     5461 gctgctgaaa ctggctacaa gggagatgaa aaactggcac aggctcgcgc taaagtccgt
     5521 gctgagctta caaaatacaa tattagcatg actgacaaag acttagacac cttcgtagag
     5581 tcagcagtga agcagatgaa tgacgcatgg aaaggacgat agggaatgga tatcgataga
     5641 aacagactac gtacaggctt gccacaggtt ggggtgcagc cttatcgaca agtacatgct
     5701 cactcaacag gtaaccgcaa ctcaaccgta cagaatgaag cggattatca ctatagaaag
     5761 gaccctgaac tagggttctt ttcacatgtt gtcggaaacg gccgtgtcat gcaagtaggt
     5821 cctgtgaaca acggaagttg ggatgttggg ggcggttgga atactgagag ttacgcagcg
     5881 gttgaactga ttgaaagcca ttcaactaaa gaagagttca tgacggacta ccgcctctat
     5941 atcgaattgc tacgcaatct agcagatgaa gcaggtttgc cgaaaacgct tgatacaggg
     6001 agtttagctg gaattaaaac gcacgagtat tgcacgaata accaaccaaa caaccactca
     6061 gaccatgtgg atccataccc ttacttggca aaatggggca ttagccgtga gcagtttaag
     6121 catgatattg agaacggctt gatgattgaa acaggctggc agaagaataa cactggctac
     6181 tggtacgtac attcagatgg ctcttatcca aaagacaagt ttgagaaggt caacgacact
     6241 tggtactact ttgacagttc aggctatatg
//
LOCUS       DBEJAQ010000077         5831 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.077, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000077 DBEJAQ010000000
VERSION     DBEJAQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.077"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(209..1177)
                     /locus_tag="ACL297_001964"
     CDS             complement(209..1177)
                     /locus_tag="ACL297_001964"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGR0072160.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1322..2105)
                     /locus_tag="ACL297_001965"
                     /pseudo
     CDS             complement(1322..2105)
                     /locus_tag="ACL297_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
     gene            complement(2130..2627)
                     /locus_tag="ACL297_001966"
     CDS             complement(2130..2627)
                     /locus_tag="ACL297_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGR0072161.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2700..4061)
                     /gene="radA"
                     /locus_tag="ACL297_001967"
     CDS             complement(2700..4061)
                     /gene="radA"
                     /locus_tag="ACL297_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGR0072162.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4075..4590)
                     /locus_tag="ACL297_001968"
     CDS             complement(4075..4590)
                     /locus_tag="ACL297_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGR0072163.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4592..5035)
                     /locus_tag="ACL297_001969"
     CDS             complement(4592..5035)
                     /locus_tag="ACL297_001969"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGR0072164.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            5340..5489
                     /locus_tag="ACL297_001970"
     CDS             5340..5489
                     /locus_tag="ACL297_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072165.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            5631..5810
                     /locus_tag="ACL297_001971"
     CDS             5631..5810
                     /locus_tag="ACL297_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072166.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
BASE COUNT         1829 a         1383 c         1032 g         1587 t
ORIGIN      
        1 cctactagaa ttctccttag aaagcgtcga ttcaagtgct atcactgttc aaaaatgatg
       61 gtcgctgaaa cttctgatga cgtatagtca tatttcttct ctttttatta tatcacagtt
      121 ttaaatctag ctttactaga ttcaccgcta ctatccattt attcggaaaa aagacgaaaa
      181 aacctgagaa tcatctcagg cttggtcatt aaattttttt ctcaatatcg aaaagtggag
      241 aaagtggtcg tttttcatga atacgtacga tagcatcccc taggagatga gcgattgaaa
      301 tctgctcaat cttatcaatc aaacgctctt ctggcagata gatggtatcc aaaacaacca
      361 atttcttaat agctgatttt tggatattgt ccgtagcagg accagaaaga actgggtgcg
      421 tacagcttgc atagactgca acagcaccag cttccgcaag agcatctgcc gcatgacaaa
      481 tcgttccagc ggtatcaatc atatcatcaa tcaagataca agtcttgcct tcaaccttac
      541 cgatgatgtt cataacttca ctagtattca tcttatcaac gctacgacgt ttatcaataa
      601 tagcgataga tgttttcaaa aattctgcca acttacgagc acgagtcacc cctccatggt
      661 ccgggctgac aaccacatag tcagaaccaa ccataccacg ccgctcaaaa taatctgcaa
      721 ttagaggagc acccatcaaa tgatccacag gaatatcaaa gaatccttga atttgcgcag
      781 catgcaagtc gatggtcaat aaacgatcca ctccagctac ttcaagcata tttgcgacaa
      841 gttttgaagt gattggctca cgcgctctcg cctttctatc ctgacgtgca tacccatagt
      901 aaggcatgac aacattgaca gactctgcac tcgcacgctt caaagcatct accataatca
      961 aaatttcaag cagattgtca tttacaggcg aactagttga ttgtaagata aagacgtgtt
     1021 tcccacggat tgattcttca atgttgacct gaatctctcc atctgaaaat tggcgaacac
     1081 ttgatttccc caactctatc ccaatctcct gcgccacacg ttctgccaat tctttattag
     1141 aagaaagggc aaacagcttt aaatcagaaa aagacatgat ttcctccggt atatatgtat
     1201 cgcttgtgct tttcacaaga ttttccatct accattgtag cgctttttgc actatttttc
     1261 aatcaaaaat aaaagaaggg caccatattt gtacccttgc atcattcttt tgaaaaatat
     1321 tctaggtcat caactcattg tgtttctcaa caaagcaata agcatgataa taaccataga
     1381 gagcaatagc cgtaaccact ggaatcgcta aaggcaactc tgtttccaac tccacaaaag
     1441 gagagttaaa caataagacc ctgtttcttg ccaaccttct gtcctttata ggctctgtaa
     1501 agcaagtaaa cacctactac agctagacct gaaaaagtcc agtgagaggc aattcctgag
     1561 atgatacgct ctaaaattcg cgaaatagta aagtcaaagc cctctggcaa atccgtacga
     1621 atataaccaa tatcctcaat catttggaat cccaaaccgg aggcaattcc aagtaaaaac
     1681 aaaaatttta attttcgcac aggaatcaaa gccaaaacaa aaacaagtgg caataatttc
     1741 aagggttctt ctaccaaagg agccacaata gcactttcaa aggcatttaa aaatggacta
     1801 tctgggaaaa gaacccccag taaatcatgt atataagtat tagcaaaact agacaaccag
     1861 cctgaaagga acatccctcc caataaagac agaatcaaaa ccttctttgg caattcccat
     1921 tttttcccaa tacggaagag aaaataaaga gccggaatca tgtaaaagag agctagaaag
     1981 atagaaactc ccattagtcc atattccgca cctgacctcg aaccgtccgt atagtagatg
     2041 gtttcatact gtaaaccaat acatagcaat aaaataaaaa taaataaaat actgcttttc
     2101 ttcatacact ttctttctaa atgaagtatt tataattcta cgactgtcat acttcctgta
     2161 tcaacattgt aaatagcacc agagataatg acatcgtctg gtattagggg agactcgata
     2221 agcagttgca tatcctcgcg tacactctct tctatatctt ggaagggcaa gaagtcctgg
     2281 tctgacacat ctacacctaa ttcctctttt aaatactcct gaaaaggttc attttcaaag
     2341 gtctgagcac cacagtctgt atggtgcaat accacaatct ctcttgtccc catttgttgc
     2401 tgggaaataa ctagcgaacg aatcatgtct tcagtcactc gaccacctgc attccgcaag
     2461 atatgagcat ccccaagtgc caagcccaga gcttgcgcaa cgtgcaaacg tgagtccata
     2521 caggtcacaa tggctactct ggttttaggt ttaagtggca gatttaactg cccatgtagg
     2581 gcaacataag cctgattggc ttgcataaac tgttcaaaat acgacacgat tccctccttg
     2641 aaaatttgat agtcaaatat ttctcctatc ttatcatttt taagagaatt tgtcacggat
     2701 tatgcaaaga cctttttcaa gacttcctga atcgttgtca cgccaatgac ctgaatttcc
     2761 ttaggcagag tgattcctgt caaggaattc tgaggtacat aaatcttagt aaagcccagt
     2821 ttagcagctt cgttgatgcg ttgctcaata cgattcacgc gccgaatctc tcctgtcaag
     2881 cccagttctc cgacaaaaca ttcctgagga ttagttggct tgtctttgta gctcgaagca
     2941 atagcaactg caacagccaa gtcaatggca ggttcatcca atttaacacc accagcagat
     3001 ttgagatagg catcctgatt ttgcaagaga agccctgccc gtttttccaa aacagccata
     3061 atcaagctag cacggttaaa atcaagtcct gtcgtagtac gcttggcatt tccaaacatg
     3121 gtcggtgtta ccaaagcctg aacctccgcc aaaatcggac gcgtcccttc catggttaca
     3181 acgatggagg aaccagtcgc cccatccaaa cgctcttcta ggaaaacttg actcggattg
     3241 agtacctcaa ccaagccgcc cgactgcatc tcaaaaatcc caatctcatt agtggaacca
     3301 aaacgatttt tgaccgctct caaaatacga aaggtgtggt gacgctcccc ttcaaagtaa
     3361 agcaccgtat ccaccatatg ctccaacata cgaggcccag ccaaggttcc ttctttggtc
     3421 acatgaccta cgataaagat ggcaatgtta ttggtcttgg ccaactgcat gagttcagcg
     3481 gtcacttcac gtacctgaga aacagacccc tgcacccctg aaatctcagg agacatgatg
     3541 gtctggatgg aatcaataat gagaaagtct ggctggatac gctccacttc tgcacgaaca
     3601 ctctgcatat tggtctctgc atagagataa aactcactat caatatcacc taagcgctct
     3661 gcacgtagtt taatctgctg ggcagactcc tccccactga catagagaac tgtccccact
     3721 tgggacaact gggttgagac ttgtaggaga agagttgatt tcccaatccc aggatcccca
     3781 ccgatgagga cgagacttcc tggtaccact ccgcctccaa gcacacggtt gaattcctcc
     3841 atctccgtct tggttcgatt gacattgatg gaagtcacct cagctagttt catgggcttg
     3901 gttttctcac ctgtcaagga cacacgcgca ttcttgacct cggcaacctc aacctcttcc
     3961 acaaaagaag accaagaccc acagttgggg caacgtccca gatatttagg ggaattatac
     4021 ccacaatttt gacatacaaa tgtcgctttt ttctttgcga tgacaaacct ctttctatat
     4081 ctctaactca cactcaatca cttggcaaaa atcaatcttc tcatttggca caaactggcg
     4141 catgagcatt cgatgagcaa caactaccac agtctgatgt tctcgatact tagacataca
     4201 ttctagaaac cgagacttca tttccgtagc tgtctcatat tgaataggac tattaggaag
     4261 caactccccc ttgttttcta aaaacagtct tctagctgtt tcaaagtttt ctattcctgt
     4321 tttatagacc tgccattcat gtaataaagg ctctactctt aaaggaagac ccgtagcaca
     4381 gaccacatac gaagccgttt ctaaagctct tgtgactgca gaagatacga ttatttcagc
     4441 tgacgagagt aaaggatttt tgctcaattt ctggacttgc tgccgtccca tctcagacaa
     4501 gggtgccaaa tctatcccaa atcctatata agaacgctcc tctaactcac ggtaatctgg
     4561 ctccccatga cgtacaaaga taatcttcat tctagtgccc tgtcgatcca aatccaccag
     4621 ttcgaacgcc atcagctgca tctccatctg caattaagaa agtagcaaaa acagcctgga
     4681 caatacgttc cccaacttca agaacaacct cttgatctgt gatattcttc atctgcgcaa
     4741 aaatatgccc ttcatttcca ggatttccat aataatcccc atcaatgacc ccaactgagt
     4801 taattaaaac caagcccttc ttacgaggat ttgaagaacg atcatagagg tagagaacct
     4861 cagtcggctg catataagcc ttaacccctg tcggaaccaa gacaatctct cctggcgcaa
     4921 caactgtacg cacagcaacc tttaagtcgt aaccagccgc atgcgctgtc tcacgcttgg
     4981 gcaataaatt ttcatctgta aaactcgaaa ccaattcaaa accacgaatt ttcataattt
     5041 tctcttttct attatcattt attctagatt attctatctt atttattcgg aaaaagcacg
     5101 aaaaaaagag cacacaacaa ttataggcga tacgataatt tacactgttt cacaatacgt
     5161 tgaaatttag agctttaaag cgagggcaaa gttgattttt tacatcgttt tacagaggtt
     5221 tacgacactt ttgccccttt tttgcccctt tgtttttcaa aaaaacttca tcaaatcgct
     5281 tgactttcac ggtttaccgt gatataatat aatcaagata aggaaaggag gtgaggaagt
     5341 tgaacaaaga agattggctt aggttactgg aaaaggcgat agataatatc cctgaaacgg
     5401 taactgctat agcaagtctg gtgactgcaa taacggtcgc aaggcaaaac aaaaagcgta
     5461 aaccgaaatc ccgtcaaaga aaaaggtgaa cgctaagagg ttggggcgaa agcccctcac
     5521 acctctattt tatcaaatga aaagaggaaa agcaatggtt agtgcaatag ctatttttat
     5581 aattgcggtc aatgtatata tttatctaaa aaataaaaag gacaaataag atgagagaag
     5641 ttattcaaga attgttagac agttcgatgt ctacatctgc tatttcccaa ggtgctggag
     5701 ttccatggac tactgtttct gacctcagaa aaggaaaaac aagcatggac aaaatggcac
     5761 ttctcacagc agaaaaactt tatgaatttg ctacaactga taagcagtga tttcggtcac
     5821 tgtttttatt t
//
LOCUS       DBEJAQ010000078         5377 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.078, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000078 DBEJAQ010000000
VERSION     DBEJAQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.078"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            168..1175
                     /gene="gap"
                     /locus_tag="ACL297_001972"
     CDS             168..1175
                     /gene="gap"
                     /locus_tag="ACL297_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGR0072167.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(1243..2118)
                     /locus_tag="ACL297_001973"
     CDS             complement(1243..2118)
                     /locus_tag="ACL297_001973"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGR0072168.1"
                     /translation="MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQ
                     EEVRWNEIVNPGDVCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
                     EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLA
                     HCKLKTGRTHQIRVHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTF
                     TTLSNTFEKELKKNG"
     gene            2205..4400
                     /gene="pbp2a"
                     /locus_tag="ACL297_001974"
     CDS             2205..4400
                     /gene="pbp2a"
                     /locus_tag="ACL297_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HGR0072169.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYNSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            4453..4605
                     /gene="rpmG"
                     /locus_tag="ACL297_001975"
     CDS             4453..4605
                     /gene="rpmG"
                     /locus_tag="ACL297_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGR0072170.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            4615..4791
                     /gene="secE"
                     /locus_tag="ACL297_001976"
     CDS             4615..4791
                     /gene="secE"
                     /locus_tag="ACL297_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGR0072171.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            4846..>5377
                     /gene="nusG"
                     /locus_tag="ACL297_001977"
     CDS             4846..>5377
                     /gene="nusG"
                     /locus_tag="ACL297_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGR0072172.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         1669 a          956 c         1169 g         1583 t
ORIGIN      
        1 ccttgattta cagtcttaaa aagaaactca catagaaaaa caatggccat acttgcaatt
       61 caactcaaat agttgtataa taggtttgtt gaaaggtgat ttgtagtgat tcaagttact
      121 cttttcacaa taaaaatttc atgattttca taaggaggaa atcactaatg gtagttaaag
      181 ttggtattaa cggtttcgga cgtatcggtc gtcttgcttt ccgtcgtatc caaaacgtag
      241 aaggtgttga agttacacgc atcaacgacc ttacagatcc agttatgctt gcacacttgt
      301 tgaaatacga cacaactcaa ggtcgtttcg acggtactgt tgaagttaaa gaaggtggat
      361 tcgaagttaa cggtaaattc gttaaagttt ctgctgaacg tgatccagag caaatcgact
      421 gggctactga cggtgtagaa atcgttcttg aagctactgg tttctttgct aagaaagaag
      481 cagctgaaaa acaccttaaa ggtggagcta aaaaagttgt tatcactgct cctggtggaa
      541 acgacgttaa aacagttgta ttcaacacta accacgacgt tcttgacggt actgaaacag
      601 ttatctcagg tgcttcatgt actacaaact gcttggctcc aatggctaaa gctcttcaag
      661 ataactttgg tgttgttgaa ggattgatga ctactatcca cgcttacact ggtgaccaaa
      721 tgatccttga cggaccacac cgtggtggtg accttcgccg tgctcgcgct ggtgctgcaa
      781 acatcgttcc taactcaact ggtgctgcaa aagctatcgg tcttgtaatc ccagaattga
      841 atggtaaact tgatggatct gcacaacgcg ttccaactcc aactggatca gttactgaat
      901 tggtagcagt tcttgaaaag aacgttactg ttgatgaagt gaacgcagct atgaaagcag
      961 cttcaaacga atcatacggt tacacagaag atccaatcgt atcttcagat atcgtaggta
     1021 tgtcttacgg ttcattgttt gacgcaactc aaactaaagt tcttgacgtt gacggtaaac
     1081 aattggttaa agttgtatca tggtacgaca acgaaatgtc atacactgca caacttgttc
     1141 gtactcttga atacttcgca aagattgcta aataattctt gagttgatag aaagcaaggc
     1201 tttgtggtct tgctttttta tatggaaaaa tggatgacac gatcatccat tcttttttaa
     1261 ttctttttca aatgtatttg aaagggtagt gaaagttagc ctctctaaag taagtgggtg
     1321 ggtaaaggaa agtcggaagg catgaagcat aagccggctt gtctttgatt tactattata
     1381 gagagggtct cccaggatag gaagattatg atgcaaaagg tgcacacgaa tctgatgggt
     1441 tcgccctgtc tttagcttgc aatgagccaa ggaagtcttg tttgagaatt gctttaatct
     1501 gcttacatgc gtttcagcat atttcccatt ttttgcatca actattcttt ttctacgatc
     1561 atggcgatca cgtccaattt tgtctctgaa aacaagttct tttctgttga tatttccatc
     1621 aactagagcc caatattctc tagaaatctc ttttttctcc aataagcgat tgagaatggg
     1681 caggataaaa ggatttttgg caaagagaac taagccactg gtttccatgt ccagacgatg
     1741 aacgacatag caggtttggc caacataggt actgacatgg ttaagaaggg caatttcgtt
     1801 tggttgatta ccatgcgttt tcatcccctc tggtttgttt acaataatca agtgttgatc
     1861 ttgataaact tcctgcacta agtctgggtt gccccaaggg atcgtctttt ggggataatc
     1921 ttcctcgtca aaagtcaact ggcaaacatc tccaggattt acgatttcgt tccagcggac
     1981 ttcttcttga tttatcaaaa tatgtttctt gattctcaaa aaatgacgga tttttctagg
     2041 gatgaggagt tgttcctcaa gtaattgctt taccgtcatt tgaggtagag aggcgggtaa
     2101 tgtaaatgtg aattgcatac agatattgta acaaaaaaag ccctatttgg ataggaaata
     2161 gctaaattct tgtcttccta tgatgaagat gataaaataa acgcatgaaa ttagataaat
     2221 tatttgagaa atttctttct ctttttaaaa aagaaacaag tgaactagag gactctgatt
     2281 ctactatctt acgtcgctct cgtagtgatc gaaaaaaatt agcccaagta ggtccgattc
     2341 gaaaattctg gcgtcgttat catctaacaa agattatcct tatactaggt ttgagtgcag
     2401 gcttgctagt tggaatctat ttgtttgctg tagccaagtc gaccaatgtc aatgatttgc
     2461 aaaatgcctt gaaaactcgg actcttattt ttgaccgtga agaaaaagag gctggtgcct
     2521 tgtctggtca aaagggaacc tatgttgagc tgactgacat cagtaaaaac ttgcagaatg
     2581 ctgttattgc gacagaagac cgttctttct ataaaaatga cgggattaac tatggccgtt
     2641 tcttcttggc tattgtcact gctggacgtt caggtggtgg ctctaccatt acccaacagc
     2701 tggctaaaaa cgcctattta tcgcaggatc aaactgttga gagaaaagcg aaagaatttt
     2761 tccttgcctt agaattaagc aaaaaatata gtaaggagca aattctaacc atgtacctta
     2821 acaacgctta ttttggaaat ggtgtgtggg gtgtagaaga tgcgagtaag aaatactttg
     2881 gagtttctgc atcagaagtg agtctggatc aagctgcgac tctggcaggg atgctcaagg
     2941 ggccggaact gtataatccc ttgaattccg tagaagattc tactaatcgg cgcgatactg
     3001 tcttgaagaa tatggttgca gcaggatata ttgataaaaa ccaagaaacc gaagctgctg
     3061 aagttgatat gacttcgcaa ttgcacgata agtatgaagg aaaaatctca gattaccgtt
     3121 acccctctta ttttgatgcg gtggttaatg aagctgtttc caagtataat ctaacagagg
     3181 aagagattgt caataatggc taccgcattt acacagagct ggaccaaaac taccaagcaa
     3241 atatgcagat tgtttatgaa aacacatcgc tatttccgag ggcagaggat ggaacgtttg
     3301 ctcaatcagg aagtgtagct ctcgaaccga aaacaggggg agttcgtgga gttgtcggtc
     3361 aagttgctga caatgataaa actggattcc ggaatttcaa ctatgcaacc caatcaaagc
     3421 gtagtcctgg ttctacaatt aagcctttag ttgtttatac gccagcagtt gaagcaggct
     3481 gggctttgaa taagcagttg gataaccata ccatgcagta taacagctat aaggttgata
     3541 actatgcagg gatcaaaacg agtcgagaag ttcctatgta tcaagccttg gcagaatcgc
     3601 ttaatctacc tgctgttgcc actgttaatg atttgggtgt tgacaaggct tttgaggcag
     3661 gcgaaaaatt cggactcaac atggaaaagg tcgaccgtgt tcttggtgtc gccttgggaa
     3721 gcggtgttga aaccaaccct cttcaaatgg ctcaagcata cgctgccttt gcaaatgaag
     3781 gtttaatgcc tgaagctcat tttattagta gaattgaaaa tgctagtgga caagttattg
     3841 cgagtcataa aaattcacaa aaacgggtga ttgataagtc tgtagctgac aagatgacca
     3901 gtatgatgtt ggggactttc accaacggta ccggtattag ttcatcgcct gcagactata
     3961 ttatggcagg gaaaactgga acaactgaag cagttttcaa tccggagtac acaagtgacc
     4021 agtgggtaat tggttatact ccggatgtag tgattagcca ctggcttggc tttcctacca
     4081 ctgatgaaaa tcactatcta gctggctcta cttcaaacgg tgcagctcat gtctttagaa
     4141 acattgccaa taccatttta ccttatacgc caggaagtac ctttacggtt gaaaatgctt
     4201 ataagcaaaa tggaattgca ccagccaata caaaaagaca agtacaaacc aatgataata
     4261 gccagacaga tgataatttg tctgatattc gagggcgtgc gcaaagtcta gtagatgagg
     4321 ctagccgggc tatctcagat gcgaagatta aggaaaaggc tcaaacaata tgggattcga
     4381 tagtcaatct atttcgttaa gatgcttgtc aaagcctagc tttcttgtta taatggataa
     4441 gatggaggcg ttatggcact aaaaaaagca agcctagctt gtgcggtttg tggttcgaga
     4501 aactattcaa tcaagatcag cggaaacccc aagcctacac gactagaagt aaataaattt
     4561 tgtaagcatt gtggcaagta cactacacac agagaaacga gataggagag agcgatgcgt
     4621 tttattggag atatttttag acttcttaaa gacacaacat ggccaactcg caaggaaagc
     4681 tggagagatt ttcgttctat catggaatac acagctttct ttgtagtaat tatttacatt
     4741 tttgaccagt tgattgtttc aggtttgatt cgatttatta acatttttta gaagattagt
     4801 ggagttaatt acactagaaa tcttctattt atgaaaggaa atatcatgga tagttttgat
     4861 aaaggatggt ttgttttaca aacttattct ggttatgaaa ataaggtgaa agaaaatcta
     4921 ttacaacgtg cacaaaccta caatatgttg gataatattc tacgcgttga aattccaaca
     4981 caaacagtgc aagttgaaaa aaatggaaag agaaaagaag tagaagaaaa tcgctttcca
     5041 ggttatgttc ttgtagaaat ggtcatgaca gatgaagctt ggtttgttgt tcgaaacaca
     5101 ccaaatgtta caggatttgt cggatcacac gggaacagat caaaaccaac tccattattg
     5161 gaacaagaaa ttcgtgacat tttggtatct atgggacaaa ctgttcaaga atttgatttc
     5221 gatgttgaga ttggtcaaac tgtacgtatt attgatggtg cttttgcaga ctacactggt
     5281 aagattacag aaattgataa taacaaagtg aaaatgatta tctctatgtt tggtaatgac
     5341 acagttgcag aagtaaacct aaaccaaatc gcagaat
//
LOCUS       DBEJAQ010000079         5302 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.079, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000079 DBEJAQ010000000
VERSION     DBEJAQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.079"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(32..262)
                     /locus_tag="ACL297_001978"
     CDS             complement(32..262)
                     /locus_tag="ACL297_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072173.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(280..921)
                     /locus_tag="ACL297_001979"
     CDS             complement(280..921)
                     /locus_tag="ACL297_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGR0072174.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(933..1655)
                     /locus_tag="ACL297_001980"
     CDS             complement(933..1655)
                     /locus_tag="ACL297_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HGR0072175.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1658..1855)
                     /locus_tag="ACL297_001981"
     CDS             complement(1658..1855)
                     /locus_tag="ACL297_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072176.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNLYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(1855..2061)
                     /locus_tag="ACL297_001982"
     CDS             complement(1855..2061)
                     /locus_tag="ACL297_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0072177.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2071..2535)
                     /locus_tag="ACL297_001983"
     CDS             complement(2071..2535)
                     /locus_tag="ACL297_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072178.1"
                     /translation="MIDKVVRNLLLTFFLCKMTKIINFLTTILVKKKKMCYDVSKLRE
                     KKKGAMMWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRLFQEMIGKE
                     PIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFDGE"
     gene            complement(2640..3929)
                     /gene="hisS"
                     /locus_tag="ACL297_001984"
     CDS             complement(2640..3929)
                     /gene="hisS"
                     /locus_tag="ACL297_001984"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGR0072179.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETISMAAYFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(4007..5254)
                     /locus_tag="ACL297_001985"
     CDS             complement(4007..5254)
                     /locus_tag="ACL297_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGR0072180.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
BASE COUNT         1737 a         1120 c          975 g         1470 t
ORIGIN      
        1 acaaaaatcc gatgaacttc accggactct tttattttga atttttgcct gctttacgct
       61 tttcagcgat ttcggctgcc tttcgaggca agacaatttc cgttatgtaa gccgtcccaa
      121 aacgcagtac acctgcaata ggagcaaaga caactgctag atagttatag aagaaatcgc
      181 ctttgaaggc ataagctagc gctccaatga tgaaaaatag aacgactgcc tgaatcactg
      241 ctaataaaat tactcgtttc atgtgacctc ctgactctat tatagcatga gaatcatcaa
      301 aaagccgact aaattattca aagcgtgaag agaaatactg tagaccagac cttttctgct
      361 aacgtaagcc aaacccaaac taaaaccaag gctaaaatag acaaaaaatt gttgcacatc
      421 acctggaaaa tgaatcaagg caaatagaag actagatacc agaagaaaaa tcagggttcg
      481 tttactattg tcctgcttag gaaagagata gcgtgctaac atccctctaa aaacaatctc
      541 ttccgtcaaa ggagcaaaaa taaccacagc aaagaatgag aaaagtggtt gagacaaggt
      601 caagtctgtc gctatttgct gatttactga aggatcatct ggcaagaaga attgaacgac
      661 cagagataag aaccaaacca aggcaggaag ccaaataaat cggttaaagc cgctcttctc
      721 aatataaaca ggagctttct gataccattt gtaaatgccg tacacatata ctccagccaa
      781 gaccacatag agtagagtaa cagcataggg tgaagcgcct aaagcaagcg acgcagtcgc
      841 gagcccctga ataaagccat agataaagaa aaaggataga agggctagaa gaatccagcc
      901 aaggttttta agtaatttca tagataactc ctttatttga aataacgttt taccataggt
      961 aactgcatca cattgatata aacatggatg gctcctacaa gcaagaaagc tagtaactga
     1021 atctctcctg tcaagaaaga aatgataata agaaaaatat ataaggctgg taagacatat
     1081 tggtgtaatt ggaataaaat tcgaaaactc tgttccaaat tagcctgacg ctccccttca
     1141 tcataagaat ttatatagtt caagacatcc tttggtgtag cgaaaaattc caaatcaaac
     1201 tgacgaacaa tcgcaatggt tttaaaaaga gatttttgag cgactaagaa taccacaaag
     1261 agtaagaaag aaaggaaaaa tgtttgaggg tttgtatgca atataatcac ctcacttaat
     1321 gaaataaaaa tagccaatgg aatcgctaca cctgtaatat taaaagcaat ggttccaaac
     1381 tcaagattcc gatacatttg cacataatag gtttcattca gatcgtcatc catttcctct
     1441 tgatacaagg aatgaaattt tctgcttttc tttaagaaat tgaaagtcaa aaacatacta
     1501 atgaaaccta tcagtaaaca aatagctgat atccatggca tcaaggcttt tacatctaaa
     1561 ataatttcgt gggattcgac acgtgcctta aacatcccta caaacatgcc caagacaata
     1621 gacatcaaaa ataacaatct acgtttcttt ttcatattca ttctcctttt tcacttgcta
     1681 gatttttgga tttcttttca atccattcaa ttactgggat gagagcaaag tagacccaaa
     1741 caaattggtc gctttgatag agattaaacc agcttaggtc catcccaatc agtagaaata
     1801 cactgactaa taaagctatg accactacat aataaatcac tttatacttg ttcatcactc
     1861 gtcctcctcc aaacgaaata ccgattcgac tgtttcgttg aaaatttgag atattttcag
     1921 ggcaatgata atggatgggg tgtactcatc ccgttctagt aggctaatgg tctgtctgga
     1981 aacccctgcc agtttggcta ggtcggtttg attgagacca tcgcgagctc taagctcttt
     2041 tagacgattt ttaagttgca tgttacacac ctactctccg tcaaattcaa cggtttggat
     2101 atcctcaatg cgttgcaact tgaatttttc ttttcccgta ttatctacac gtcgtagctt
     2161 tacccattcc tcatcaacat ccacaacttc ccagttatct ggcccaatat acactcccgt
     2221 tataattggt tcctttccaa tcatttcttg aaataatctc gacatttctg cgtttccttt
     2281 ctcttttcgc tcaagttttt tgattttatt ctctagtttc ttgatttttt tagaattatt
     2341 agaataaaag aaaatcataa atagtataaa tcctagtacc cacatcatag ctcctttctt
     2401 cttttctctt aacttgctta catcgtaaca catctttttc tttttgacaa gtatagttgt
     2461 caaaaagttt atgatttttg tcattttgca aaggaaaaag gtcaggagta agttcctgac
     2521 cactttatct atcattaata ctcttctaaa atctcttcaa accacgtcag cttcatctac
     2581 aacctcaaaa ccatgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
     2641 cattgagtat aaaatcctag tttttcaaag atttctgaga agttttggct gattgtctca
     2701 agtgacactt gcacttcttc tcgggtttgg ttgttcttga ccgtcacttg tccgctttcg
     2761 acttcgctct ctcctagggt gatgagggtc ttagccgcaa agacatctgc tgacttgaac
     2821 tgagctttga gcttacggtt gaggtaatca cgctctgctt tgaaaccttg ttggcgaaga
     2881 gcttggacta gttctagggc tttgacattt gctccatcac ccaagactgc gatatagaca
     2941 tctagggcgt tttcgatagg gagggccacg ccttgttttt caaggatgag aagcaggcgc
     3001 tctacaccaa gcccaaaacc aaatccagca gtttcagggc ctccaaagta agcaaccaaa
     3061 ccatcgtagc gaccacccgc acagactgtc aagtcattgc cctcaatctc tgtgataaac
     3121 tcgaaaatgg tgtggttgta gtagtccaga ccacgcacca tattggtatc gatgatgtaa
     3181 tctactccaa gattttccaa catctgacgc acagcatcaa aatgagtttg gctttcttca
     3241 tcaagaaagt ccaagataga cggcgcattc tctactgcca ccttgtcttc tttttcctta
     3301 gagtccaaga cacgaagagg attttcctcc aagcgacgtt ggctatcctt agacaaggtc
     3361 tccttgagcg gtgtcaaata gtcaatcaag gcttggcggt aggctgcacg gctctcagga
     3421 tttccaagag tgttgaggtg caatttgaca ccttgaatac cgatttcctt caaaaaatag
     3481 gctgccatag agattgtttc cacatcggta gctggattgc tagagccaaa acactcaaca
     3541 ccaatctggt ggaattggcg caagcgccct gcctgtggac gctcataacg gaacataggt
     3601 cccatatagt agaacttgct tggcttttgc acttctgggg cgaagagttt attttccaca
     3661 taggaacgga caacgggcgc agttccttct ggacggaggg taatatgacg gtcacccttg
     3721 tcataaaaat cgtacatttc cttggttacg atatccgttg tatctccgac agagcgactg
     3781 ataacctcgt aatgctcaaa aataggcgtg cgcacttctg catagttata gcgcttgaaa
     3841 atctcacggg caaagccctc aacgtactgc cacttagcag attcagcagg taaaatatcc
     3901 tgcgttcctt ttggtttttg taatttcata gggaatcctc tttaaactta atagtcttat
     3961 tttaccacaa ataaagggat taaaacagta agaaaaaaat taggatttag atatcatttt
     4021 tgagattaag aattgtcaaa aaaatagcta gcaaggaaag accaacaaat agcatccaag
     4081 tcaactgtat attccatacg gctactagtg aaaaacaagc tgttcccaca ggtatggata
     4141 aggtaaacaa tagacctaaa aaattactag tacgagctag aacctctgga gctagatttt
     4201 tcatgagcat ggcactaatc tttggttgaa ctttaccaga cacatacagg gtaaagaaga
     4261 gaaatagcaa accaagcacg acttgattga ataaattagc caaaccaact agactaagtc
     4321 ctacggtcgc ccacatcatc aatctaggca aggactgctt cccaaaataa tcattgcccg
     4381 taaggctact gatgatgatt gctactaaaa cacagaattg attgataaat agtgcctctg
     4441 tataagaaaa attcaagaga gaatggctca aaaagaagat attataaatt ccacccaaag
     4501 cgccacccaa ggaattaata agcaagacag caaagagcat aaaaccaaag tttttctgcc
     4561 cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt taggaactgg tctttgatag
     4621 aaagcttctc attttttaag ttttcaccat cagcagatga cattgacagg ctcaatttgc
     4681 tttttcctaa aaagaggata gtggctgata ctaggaaaaa gcaggcattg attcccgcaa
     4741 cgagagaaaa attgttgacc gatagagcta agagccagac tccgaaagct tgaccaccaa
     4801 tagctgaaat ataggtgatg aactgtgaaa aagaataagc ctccatcaga tcatcttcag
     4861 ctactttttc cttaataaga ggcatgcgca agccacctgc aaaatcactg atgacatcac
     4921 taatgacatt gatcaaacac aggctagaaa aggcaaagag actagcttgc tgaacaacta
     4981 gggctgctag gaaaaataga accgcctgaa acaaaccgct atagaccatc catttgacct
     5041 tgtccctcgt gtaatctgcc cgaatcccta caaaaactgt aaagagggtc ggaagaatca
     5101 tgacaatatt cgccatagca acagcaaaag atgcttgtga caaggtcgat gcatagacga
     5161 taaagaccag gttgaaaatc gaagcaccaa aagcattgaa gaagcgtgat aaagtcaaaa
     5221 ttctataagc tggatttcta aacaatagtt tcatagataa tctcccttct tgttctaagt
     5281 tctacatcaa gaatagcttt ag
//
LOCUS       DBEJAQ010000080         4844 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.080, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000080 DBEJAQ010000000
VERSION     DBEJAQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.080"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            21..383
                     /locus_tag="ACL297_001986"
     CDS             21..383
                     /locus_tag="ACL297_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGR0072181.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            782..1027
                     /locus_tag="ACL297_001987"
     CDS             782..1027
                     /locus_tag="ACL297_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072182.1"
                     /translation="MKAFYITIEKFIRDDESLKCYFTKVISSRSKVTSLDQIEADKTI
                     QRKYSSELKKFIGFYNEIICEENSFLHVRKRWSSWFR"
     gene            1428..2420
                     /locus_tag="ACL297_001988"
     CDS             1428..2420
                     /locus_tag="ACL297_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0072183.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2422..3240
                     /locus_tag="ACL297_001989"
     CDS             2422..3240
                     /locus_tag="ACL297_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0072184.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3242..4027
                     /locus_tag="ACL297_001990"
     CDS             3242..4027
                     /locus_tag="ACL297_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0072185.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4125..4343
                     /locus_tag="ACL297_001991"
     CDS             4125..4343
                     /locus_tag="ACL297_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGR0072186.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4328..4489
                     /locus_tag="ACL297_001992"
     CDS             4328..4489
                     /locus_tag="ACL297_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGR0072187.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
BASE COUNT         1397 a          792 c         1017 g         1638 t
ORIGIN      
        1 ttttcattga gtataacaca ttgttagaag ttggtttaaa tttcctaatc agtttgttca
       61 catttacctt cgatatatta tatcccatag ttaaggttgg tcatacagat gattatagcc
      121 atggagccgt aaaacttagt gtttctttag ttgacaaaga tgccatgaaa aaaatatctg
      181 taactgtaat aggatatttt gaaataaata tagatgaaaa tatcaccgat attctatacg
      241 taaatggtac tgctattctt tatccttatt tacgttctat tgtttcaata gtttcggcaa
      301 ttgatagcag tgaagcaatg ttgctaccta ccattaatgt tttagagtta ctagataaat
      361 ctcaaccttt tgaagaagaa taatttatta gctcactaaa ttgagggtaa ggaaaagtaa
      421 aagcagtaag aaaaatgtct tgcattatac agcaactttt tgggaatgag tagatggatt
      481 gaataaaatt tgattaagag tggatgattt atctgtagat tattattgga cagttagtct
      541 tgaagtagtc taagaattag gttataatca gtagaagcct tgctaataat gaggaggtta
      601 gtttatgtat agtagactga atctaaaata gtacgaaaca attgctaaaa catttataga
      661 aattaatttt actttcccaa tcgatttgtt ctcatcttat ttcaatccgc tatatattat
      721 ggtatcgaat cttcatcaga atgataaaat taatcaatta atatctgatt acaaacagaa
      781 tatgaaagct ttttatatca ctattgaaaa atttatacga gatgatgaaa gccttaagtg
      841 ttattttaca aaggttattt caagtcgttc caaggtaaca agtctagatc agattgaagc
      901 tgataaaacg atacaaagaa aatattcaag tgagctaaaa aaatttattg gattttataa
      961 tgagattatt tgtgaggaaa atagtttcct acatgtacga aagaggtggt cgagttggtt
     1021 taggtagtcg gtgcgtgagt tgataattct cagggtatgg acttcttttt catgaatgag
     1081 gtaaaagagc aggtattgtt tagagacaat cattctgagc atattttctg gatagaggga
     1141 gtatccgatt ttatgatcaa agttaatacc gccctctggt gagaagatga gtaggttggt
     1201 aatttaaact attaaacaga atttttgatt aaaagtatta tttcatgaga gaaatcctaa
     1261 tttcacaatc tataggcaaa cgcttgcatt tcgtttttta ttggactaca ataggttggt
     1321 ataaagcctt ctgtagtaat aaaatgtaga aggtgtagaa agtaaggatt tagaatattt
     1381 gtagttaaaa acacaatgtt gctattcctt acgataggga gatagatatg gcaatgatag
     1441 aagtggaaca tcttcagaaa cattttgtga agattgttaa ggaaccgggc ttgaaggggg
     1501 ctttgcgctc ctttattcat cctgaaaagc agacctttga agcggtcaag gatttgacct
     1561 ttgaggttcc aaaagggcag attttaggat ttatcggggc aaatggtgct gggaagtcga
     1621 caaccattaa aatgctgaca ggaattttga aaccaacatc tggtttttgt cggattaacg
     1681 gcaagattcc ccaggacaat cggcaagatt atgtcaaaga tattggcgta gtctttggac
     1741 aacgcaccca gctatggtgg gatttggctc tgcaagagac ctacactgtc ttaaaagaga
     1801 tttatgatgt gccaaactcg ctctttcata agcgtatgga ctttttgaat gaagtcttgg
     1861 atttgaagga ctttatcaag gatcccgtgc ggactctttc actgggacaa cggatgcgag
     1921 cggatattgc ggcctccttg ctccacaatc ccaaggttct ttttttagat gagccgacca
     1981 ttggtttgga cgtttcggtt aaggataata ttcgtcgggc aattactcag atcaatcaag
     2041 aggaagaaac taccattctt ttgaccactc acgatttgag tgatattgag caactttgtg
     2101 atcggatttt catgattgac aaggggcaaa agatttttga tggaacggtg agccaactca
     2161 aggagacctt tggtaagatg aagactctct cttttgaact gctaccaggt caaagtcatc
     2221 tcgtctctca ctatgaaggt ctgtctgata tgaccattga tagacaagga aacagcctca
     2281 acattgaatt tgatagttct cgctaccagt cagctgacat tatcaagcaa accctatctg
     2341 attttgaaat ccgcgatttg aagatggtgg atacggatat tgaggatatt atccgtcgct
     2401 tctaccgaaa ggagctctag gatgatcaaa ttgtggagac gttataaacc ctttatcaat
     2461 gcaggggttc aggagttgat tacttaccga gtcaacttta ttctctatcg gattggcgat
     2521 gtcatggggg cttttgtggc cttttatctc tggaaggctg tctttgattc ttcgcaagag
     2581 tctttgattc agggcttcag tatggcggat atcaccctct acatcatcat gagttttgtg
     2641 accaatcttc tgactagatc cgattcgtcc tttatgattg gggaggaggt caaggatggc
     2701 tccattatca tgcgtttgtt gcgaccagtg cattttgcgg cctcctatct tttcaccgag
     2761 cttggttcca agtggttgat ttttatcagc gttggccttc catttttaag tgtcattgtc
     2821 ttgatgaaaa tcatatcggg tcaaggtatt gtagaggtgc taggattaac tgtcctttat
     2881 ctttttagct taacgctcgc ctatctgatt aactttttct ttaatatttg ctttggattt
     2941 tcagcctttg tgtttaaaaa tctttggggt tccaacctac ttaagacttc catagtggct
     3001 tttatgtcgg ggagtttgat tcccttggca ttctttccaa aggttgtttc agatattctc
     3061 tcctttttgc ctttttcgtc cttgatttat actccagtta tgatcattgt tggaaaatac
     3121 gatgccagtc agattcttca ggcactcctt ttgcagttct tctggctctt agtgatggtg
     3181 ggattgtctc agttaatttg gaaacgggtc cagtccttta tcaccattca aggaggttag
     3241 tatgaaaaaa tatcaacgaa tgcatctgat ttttatcaga caatacatca aacaaatcat
     3301 ggaatataag gtggattttg tggttggtgt cttgggagtc tttctggctc aaggcttgaa
     3361 tctcttgttt ctcaatgtca tctttcaaca tattccattc ctagaaggct ggacctttca
     3421 agagatagct ttcatttatg gattttcctt gattcccaag ggaatggacc atctcttttt
     3481 tgacaatctc tgggcactag ggcaacgcct agtccgaaaa ggagagtttg acaagtatct
     3541 gactcgtccc atcaatcctc tctttcacat cctagttgaa acctttcaga ttgatgcctt
     3601 gggtgaactc ttagtcggtg gtattttatt gggaacaaca gtgaccagca ttgtttggac
     3661 tcttccaaaa ttcctgcttt tcctagtttg tattcctttt gcgaccttga tttatacttc
     3721 tcttaaaatc gcaacagcca gtatcgcctt ttggactaag cagtcaggcg ccatgattta
     3781 catcttctat atgttcaatg actttgctaa gtatccgatt tctatttaca attctcttct
     3841 tcgttggttg attagcttta tcgtgccttt cgcctttaca gcctactatc cagctagcta
     3901 tttcttacag gaaaaggatg tgttctttaa cgtaggaggt ttgatgttga tttctctggt
     3961 tttctttgtt atttccctta aactttggga taagggctta gattcctacg aaagtgcggg
     4021 ttcgtaaaag ctaaagtaag actaaaatca agaaaggaac ttatgatgtt tgtaattgaa
     4081 gaagtcaagg atgaaaatca aaaaaggcag ttgtcgttga ggttttgaag gatttgccag
     4141 aatggtttgg aatcccagaa agcacacaag cctatataga aggaaccacg acactgcaag
     4201 tttggaccgc ctatcaggag agtgatttga ctggatttgt aaggttatcc tattcgagtg
     4261 aagattgtgc agagattgat tgtctcggcg taaaaaagct tatcaaggta gaaaaattgg
     4321 gagccaattg cttgctactt tagagagtga agctcgtaaa aaagttggtt atctgcaggt
     4381 caaaacagtg gcagaaggtt ctaataaaga ttatgatcga acaaatgact tttatcgagg
     4441 tcttggcttt aaaaagttag agattttttc tcagctatgg aatccgtaaa atccttgtca
     4501 gattttgatt aaaaagcttg aataatatta cttgacatct attctcagag tgccatactg
     4561 taagtgtaat cgccgattta gctcagttgg tagagcaagg cactcgtaaa gcctaggtta
     4621 taggtagata aacgactgag gagttgaaaa aatagatagg tagaagataa ccgttaagcc
     4681 ttattcttag cggttattta tattgtttaa tagcgctgat atttcatcaa ttatgcctgt
     4741 tttcgtgttt ctggtagttg ttcaagttta ttgctactat ttttgatggt atgaatgtgc
     4801 ttataatgta tcccggttaa cgaaagtttt ggacttatac tcat
//
LOCUS       DBEJAQ010000081         4815 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.081, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000081 DBEJAQ010000000
VERSION     DBEJAQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.081"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..220
                     /locus_tag="ACL297_001993"
     CDS             <1..220
                     /locus_tag="ACL297_001993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072188.1"
                     /translation="RFNNMAPSPKIIKKPHNLPRQSLIVKLSLIRNQPLKRPIFYTLF
                     YTMKGVMSIFSMFRPCPEADKQGEYNEI"
     gene            210..1358
                     /locus_tag="ACL297_001994"
     CDS             210..1358
                     /locus_tag="ACL297_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGR0072189.1"
                     /translation="MKYNKTKYPNIFYYETAKGKRYYVRRSFFFRGKKREKSKSGLTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIQAKFGSTLLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATELHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(1600..2886)
                     /locus_tag="ACL297_001995"
     CDS             complement(1600..2886)
                     /locus_tag="ACL297_001995"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGR0072190.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(3117..3353)
                     /gene="comW"
                     /locus_tag="ACL297_001996"
     CDS             complement(3117..3353)
                     /gene="comW"
                     /locus_tag="ACL297_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma(X)-activator ComW"
                     /protein_id="HGR0072191.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            3619..4416
                     /locus_tag="ACL297_001997"
                     /pseudo
     CDS             3619..4416
                     /locus_tag="ACL297_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4451..>4815)
                     /locus_tag="ACL297_001998"
     CDS             complement(4451..>4815)
                     /locus_tag="ACL297_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGR0072192.1"
                     /translation="GRRYQRISLVAGLTNGELIAPMTYEEMMTSDFFEAWFQKFLLPT
                     LTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAYIKKNLKKV
                     LPSCNTFYEALFSCSCFN"
BASE COUNT         1680 a         1027 c          767 g         1341 t
ORIGIN      
        1 tagatttaac aatatggcgc ctagcccaaa aataataaaa aagccccaca atctccctcg
       61 ccaaagtttg attgtgaagc ttagccttat aagaaatcag ccattaaaaa ggcctatttt
      121 ctatacccta ttttacacca tgaaaggggt gatgtcaata ttctcaatgt ttagaccttg
      181 tccagaagcc gataaacaag gagaatacaa tgaaatataa taaaacaaaa tacccaaata
      241 tcttttacta tgagactgcc aaaggcaagc gatactatgt cagacgttct tttttcttcc
      301 gaggtaaaaa aagagaaaaa agtaaaagtg gtctcacaac ccttccacaa gctcgtgcag
      361 ccttagtaga gcttgagcaa caaatccaag aacaagaatt aggtatcaat acgaatctga
      421 cacttgatca atattgggat atttattctg aaaagagatt gtcaacaggg cgctggaatg
      481 acacttccta ctacctcaat gataatctct acaagaacca tatccaggca aagtttggtt
      541 ctaccctgct taaaaatttg gatagaaatg agtatgaact ctttatcgct gaaaagttgc
      601 agaaccatac cagatacact gttcaaaccc tcaattccag cttcatggca ttgctgaatg
      661 atgccgtcaa aaatggaaat ctgctctcaa atcgcttgaa aggtgttttc atcggccaga
      721 gtgatatccc tgctgcaaac aagaaagtga ctctcaaaga gttcaagact tggatagcaa
      781 aggcagaaga gattatgcca aaacaattct acgctctgac ctatctgaca atttttggat
      841 tgagaagagg agaagtcttt ggattgcgtc caatggacat cactcagaac gacagcggac
      901 gggctatact gcatcttaga gacagtcgaa gcaaccagac cttaaaaggg aaaggagggc
      961 ttaaaacgaa ggattcagag cgatatgtct gccttgatga tatcggaaca gacctgatct
     1021 attatctgat agctgaagct tctaagatta agcgaaagtt aggaattatc aaggaacagc
     1081 acaaggatta tataactatc aacgagaaag gtggtctcat caatccaaat cagctaaata
     1141 gaaacttcaa tctagtgaat gaagcaacag aattgcatgt aacacctcac atgatgcgcc
     1201 acttcttcac gactcaaagc attattgcag gggttccgct tgaacaatta agccaggcgc
     1261 tgggccatac aaaagtctat atgaccgatc gttacaatca agtagaggac gaacttgctg
     1321 aagcgacaac agacctattt cttagtcata ttcgctaaaa aaatccccgc caattccccg
     1381 accaaaatcc gaaaaatacc gaaaaatatc gaaaaattat ttttagaata gtccccaaaa
     1441 gcctgaaata aagctaaaaa actccacctg attgggtgga gttaagggag attattatga
     1501 aaaagaaaag tttaggatat ttgttacaac aagttaggag gtcttcttgt aactgtctat
     1561 agtatacccg acctatctta aacaaatctt aaaaatctct taggaccaaa cactttctaa
     1621 aatatttgtt tgttcacgac caggacctac tgagaaagta gaaatacgaa cgccaaccaa
     1681 ttcactcaca cgacgaacat agttacgcgc attctcagga agatcttcca aattgcgaac
     1741 tccggtaata tcttctgacc aacctggcaa ctcttcatag ataggcttgc aacgtttcaa
     1801 ttgctcaaga ctagctggat agtagtcaat acgttgaccg tcaagatcat aggccacaca
     1861 gattttcaca gtatccaaac cgctcaaaac atcaatagag ttcaaagaaa ggttagtaat
     1921 accagaaaca cgacggctat gacgcatcac aactgagtca aaccaaccta cacgacgtgg
     1981 acgaccagtt gttgtaccat actcatgacc cacttcacgg atacgttctc ccacttcatc
     2041 aaacaactca gttgggaaag gaccatctcc tacacgactc gtataagctt tacatacacc
     2101 tacaaccttg tcaatcttgc ttggaccgac accagaacca attgtcacac caccagctac
     2161 agggtttgat gacgtaacaa atggataagt accttggtcg atatctagca taacaccttg
     2221 tgcaccttca aaaagcacac gtttgccatt atcaagcgca tcattcaaga taacagatgt
     2281 atctatcacg tatttcttga tttgttgacc atattcgtaa tattcttcaa aaatatcatc
     2341 gaaaacaatc gctttactgt catacaattt ttcaaaaaga cgattctttt cagcaaggtt
     2401 acgttctaaa cgctcacgga aaatatcttt atctaaaaga tctgcaatac gaattccaac
     2461 acgagcagcc ttgtccatat aagctggacc aattccctta attgtcgtac caatcttatt
     2521 gtcgccctta gcttcttctt gcaagcgatc caactcgata tgataaggca aaataacatg
     2581 cgcacgatca gaaatacgca agttatcagt tgttacacct tcctcatgaa gatagctcaa
     2641 ctcttttaca agagatttag gatttacaac cataccattc ccaatgacag atattttttc
     2701 agggaagaaa atcccagatg gaatcaagtg caacttaaat ttcttaccgt caatcacaat
     2761 cgtgtgacca gcattatcac caccttggta acgtgcaatc acttctgcat tcgctgaaag
     2821 gaagtctgta atcttccctt taccttcatc accccattgg gtacctacaa caacaactga
     2881 agtcataatt ttgtctgagc cctcaggctc ttcctttctc acatacatgg caggactctc
     2941 acctgcaatt atatcttaca atttattata ataaaaaatc gcctttttat caagaagaaa
     3001 caatagaaag atttgctatt tccaactatt aaaaaatgat ttagaaaaat tactagctat
     3061 ttactattat ctttacataa aagagtaaat tagttcggaa atttactaaa attacctcaa
     3121 caagaaataa aaccccgatt cattaccaat ttttcaagat acaaacgata agcaacacga
     3181 taatggtaaa cgataaaatc cctatgacaa ccaatgccat atctcactaa ataataaatt
     3241 aaaaatttaa aatgaacatg ttcccagtca aaattatcac caaatgtagg accatactct
     3301 tcttcaatac tatcatagaa attagccatc tgctcataaa ttttttgtaa cataatcaaa
     3361 tactcctttt cttttttata aacttattct aacaaaaaat tttacacatt cactatcaat
     3421 tcctgaattg ttaaaatacc agcctcaaca agataaaaat aggaaaagtt gacaaaatag
     3481 aaacaaattt gctacctaaa tttcaaacaa ttaaagttct aaaaagaccg ccttaaaact
     3541 tttgggtaaa tcctattaga actataatat ttttatatag gtattatggt tctgattttc
     3601 tagtataaaa aaattcaaac tagaattctt cttagaaaag gccgttttaa gtgctatcat
     3661 tgctcaaaaa tggagctcgc tgagacttct atcgtcaaga agaatcatca aattccttgt
     3721 atcatcaacc aaaagattgc tcaaaagtta attgaaaaga tttctatgac cgacattgat
     3781 catcagttgt ctatttcaac ttcaactgtc attcgcaaga tcaataattt tcactttgag
     3841 catgattttt cgcgtcttcc tgagattatg tcctaggacg ttgaaacagt ccggggagtg
     3901 actgtttcaa tcgggagatg gagatgagct ttattgctca ggactttgat aaactcaata
     3961 tcataactgt tcttgagagc agaacacaag ccatcatccg aaatcccatg aatacaaggc
     4021 tatcaagcgc tactggaagc tcattcaaca agatagtcag aaactgagtg ataaacgatt
     4081 ttatcgccct acttttcgta tgcacttgac taataaagaa attctagata aacttttgag
     4141 ctattccgaa gacttgaaac accactataa tctctatcag ctcttgcttt tccacctctg
     4201 tttcagactt tgtttaaaac cttcctcaaa gataaagaga aaatcgtcaa cgcccttcaa
     4261 ttaccttatt ccaacgccaa actggaagcc accaataatc tcatcaaact tatcaagcac
     4321 aatgcctttg gttttaggaa ctttgaaaac ttcaaaaaag aaaggacgaa atttgtcctt
     4381 tctagatctt cgctttcttc aacccactac agttgacaaa gaaccgaaaa atgtctcgcc
     4441 tctaatatag tcaattgaaa caagaacaag agaaaagagc ctcgtaaaag gtattgcaac
     4501 ttggtaatac ctttttgagg ttctttttga tataagccca tgttttctca ataggattgt
     4561 actcaggtga gtagggagga agaggtaaaa gtttatgccc aaactcctcg cataaaagtt
     4621 ctaacttacc cattctatgg aatcttgcat tatccataat aataaccgat ggtgtggtta
     4681 atgttggtaa gagaaacttc tgaaaccaag cttcaaaaaa gtcgctcgtc atcatctctt
     4741 cgtaagtcat tggagcgatt aactcaccat ttgttagacc tgcaaccaaa gaaatcctct
     4801 gatatcttct tccag
//
LOCUS       DBEJAQ010000082         4719 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.082, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000082 DBEJAQ010000000
VERSION     DBEJAQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.082"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(779..1039)
                     /locus_tag="ACL297_001999"
     CDS             complement(779..1039)
                     /locus_tag="ACL297_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072193.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
     gene            complement(1098..1649)
                     /locus_tag="ACL297_002000"
     CDS             complement(1098..1649)
                     /locus_tag="ACL297_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072194.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1639..2634)
                     /locus_tag="ACL297_002001"
     CDS             complement(1639..2634)
                     /locus_tag="ACL297_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072195.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHILKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(3042..3467)
                     /locus_tag="ACL297_002002"
     CDS             complement(3042..3467)
                     /locus_tag="ACL297_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072196.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(3611..3955)
                     /locus_tag="ACL297_002003"
     CDS             complement(3611..3955)
                     /locus_tag="ACL297_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGR0072197.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKK"
     gene            complement(4001..4207)
                     /locus_tag="ACL297_002004"
     CDS             complement(4001..4207)
                     /locus_tag="ACL297_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072198.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(4194..4355)
                     /locus_tag="ACL297_002005"
     CDS             complement(4194..4355)
                     /locus_tag="ACL297_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGR0072199.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT         1540 a          922 c          638 g         1619 t
ORIGIN      
        1 aactaggatt ctagttttgc aggaagtcta ttatatatat gcacagacat atgaaatctt
       61 tttgatacag ccttcttttt acatagagag acagcagtta tgatatattt atccattcac
      121 aaaatcttct gtaccaatac tacaataatt ggtacatttt gattacacaa tcgtttcaaa
      181 aaatatgatg gaagactgag tattttatag taattccctt cctatcaact agatcaacca
      241 gttgagtgtt gaatgttgaa acaccctagc ctcagcaaag gactatgatc agcatttcct
      301 agccaatagg acgctccaaa tcacctattc taagtccatc actagcttgg tcagtaagat
      361 acagactgca aggcaagact gacgtggttg atgagatttt ggaagagtat tacaatcata
      421 gattcaccag tagacttgac cgcagattta actatagtag attgaaacta gaatagtaca
      481 cttctgcttc taaaacattg ttagaaatcg atttgactgt cctgatcaat ttgtcctgtt
      541 ctcgtttcat tttactatac taaaaaacca cctgaaacaa atcaagtgac tagaggataa
      601 aaacaaagac tgggcaaaaa gttttgaaaa gcaaaaaacg cataatatca ggtcttgaaa
      661 aaccttgcta ctatacgttt gattgtggga agattgactt cattttctcc tgaaattgaa
      721 gttttttcta gtttccaggt atagacgaat tcaatatagc catctcgcta caattatttt
      781 aaccatcgaa aaaatttttc agaatttcaa aaatttcatt ttgatccaat gaaatgtcat
      841 tattttgaag aatagttgac ttcacaggct ctaattctat cacaaagcgg tgcatgtctg
      901 cttttaccgc actacttgat gttatagccg ataacttttc atctcctagc aaggaagctg
      961 tcaaacgggt aaggtcattc aaatgctttt taatggactt acttaaacct tgagcacctg
     1021 tggcagagcg ctccctcata tccagccaag ctttagcctt aaaaataatt aatactcaat
     1081 gaaaatcaaa gagcaaacta ggaagctagt tgcaggctgc tcaaaacact gttttgaggt
     1141 tgtagatgga agctgacgtg gtttgaagag attttcgaag agtatagacc acaattactc
     1201 aataccgaat acccctgaat ggtttctttt acatgcacca atatattata ataatcttca
     1261 tccaataata aggctgataa actagcatct tggtcaaaat ataagggaat ttctcgaccg
     1321 tccttcttta atggatattc tggtaagata ctaaatagtt caatcataga aggaaactca
     1381 gaattagttg ttgtaaatcg aaaaagctgc gctttctcat ctttctgact tccttgatac
     1441 tctcccaatt ctaaaaaatg attcaaggta gtataaaatt ccttattttt tacttcatca
     1501 atgatgacca tatcataatc ttttgttgtg cgacttttaa atccttgcga atccaatacg
     1561 atagaggtag cagttccccc aatcagaaca taatagttct gaaaatccgc aaacatttct
     1621 tgaaaaataa ctttcgtatt agctggcatc atcttctccc agatactgta atatcatatt
     1681 ttctagtgct tcactctctt cctctatacg tgggtcatca tcatctttta aggtcaaata
     1741 aagagaaatc ggatctacaa attgtttatc atgattattt ttaaaatcat tccaaaactc
     1801 agatacaaaa ggacgatatt tccatatctc tagcatcttt ccttttaaaa tatgctgaga
     1861 aagtggcaag gataactgat tgaattttct ctgccatatg acatagctag tattttcatc
     1921 cgtttcagct aaaaaagttg aatgcgacaa agcataagca ccaccatata gaaggttaga
     1981 aacagatttt atctgcttta tatcgccatc tggcaataaa atccgttttt tgatgggatt
     2041 aaataaacat gacacggatt ttaagaataa ttctttcttt gacaccgtat atgtgtaaag
     2101 cttattttgc ttgtttaacc aatataaagc tttaaaagtc ctcaaacacc tataaattgt
     2161 tgagtttgga agtccagtga cttgtgaaag caaatcaaca tctactactt tttgaccttt
     2221 tgtcaataaa aaggcaatcc acgttaattg ttcgctaggt gttaattcct tagggacttc
     2281 agtatcattc gcattgagta ctagtcccaa tggagggaag aagaggtttc ccttaaagtc
     2341 tacaaacgga actctagctt gaagtaattg ctttttttca ctgtctgata acttcgaaaa
     2401 caccaaaaca acatccatat tggctttttc acccatagtg cgagcctgag taacaaatga
     2461 actcaaactc ccccttctct tttctttaat caataaaaat gattccttat gaaaagtcat
     2521 ttgagtatac tcaaaccttt ttacaaaact gattggtaaa gtcaaagtca gttcctcaat
     2581 actgacatca acataaaagg attgaaaaat tgacattatc tttttgacat tcatgttttt
     2641 tccttattct atcattttta ttatatttta tcattcggta taataaaata taagtttttt
     2701 gataaaataa ccaaaaacta ctccctatcc cctctcacat taccctacat atcgtttgac
     2761 atgcgactga tagttcagga aaacttcaag gagaactttt ctctcatcca ctatgcagga
     2821 cttactattt cacttctact cctataggct caatttgaca cttttctttt tcgaattgct
     2881 catatgcctc ctgaatagtg agttcgattt atccaaattt atttttgtct attttgatga
     2941 atacttccac gaagatagtc tgaaaatgaa tttgcaccaa ttttccttct ctttttaagc
     3001 atattattcc agatatgatt gccctcttgt tcagtaataa attacgcttt aaacgcttca
     3061 atcagtatat ctcctgttgt catatgttct aaagaaaatt cttctacata tgatttaaca
     3121 tctcttaggt tattacttcc taatatccca ttatgctttt tcgctaagga aatagaagcc
     3181 gcttctccct taccaataat cttgttacta tcatgatttc ttgttaaatc tctatataat
     3241 gcgtattctt cagttccaat gtctatgctc acaatctcag ctgaaccctt agctaccaac
     3301 tgatctatcc tagattttaa atggggaatt gtaggtatat tgatttcatc atacacctct
     3361 tgtggaataa caattttacc cgaatagagc ttttctaaaa gatgttcagt accaacccat
     3421 aaaaatactg aaatacaatc tgcatcaata aatacacgac tagtcaaacg acaactcccc
     3481 cctcttcatc tagcccatat acaatatcat atctgaaagc atctagtaac agttcctcat
     3541 acttcccttg cgaaattctg ttattttcta aaagttgttc agtttaatta acatattatc
     3601 ctaatatcat ttacttttta ctttctgaca aaggacgata taaacttgta tcatagccta
     3661 atcttgaagc tgtctctata acactaatat ccatattttt aatttcttct gcatcaaggt
     3721 atccatcatt cctcaatcta tataacataa ctttatgact gataccataa aactgaccca
     3781 attttataat atcttctact tcaagatgag ttctattggc attttctcta atttcctcaa
     3841 ccatcctata cagtgaagat gggaaaatta aaaaataaga agcaaactga tccgcttttc
     3901 tttcagtttc atctccttca ccaatcaaga taagactgac tgaactcttc ttcacctcat
     3961 cataataaag atgatacagt tcatgtgcta aagaaaatct ttaccttcct aatgacatgt
     4021 ctgaattgac tgcaatgaga ctgaactgag ttcctttata acagactccg ctaatattct
     4081 ttccgagtcc ataaaatacc agcgtcaaat tttctatctt ttgtaccaat ttaaaaatat
     4141 ctatcggcga ttcaccatca gctcccaact tttttctaag atttgaagct ttattactta
     4201 aatccattcg tgatattcct ttcacctttt tccattttgg caatcatgct ttgattcaaa
     4261 gacaaatact cagcaatctt atcttgagta agatttgaag atattctaag ttcttttatt
     4321 ctttttccaa catcacatac atttatcaca tacatttatc atattcatac acctctcata
     4381 aaaagaatag cacacttttg tcataatttt ttaaataaaa aaattatgac aaaacaagga
     4441 agcaatttat tgatgctgct taaaaatcta aaattgatga gttcctatca aaaatcgtat
     4501 cttcaacctc aaaacagtac ttaaagctat ccgactcggt ttacattgtc aaatttagat
     4561 tttatttgag cataactttc tagtttgctt tctgattttt gtttaatata gtagcaaaca
     4621 ttgaaggaaa tatctccaca aaaaaacgca tactattaag ctttttcaag acctaataat
     4681 atgcgctgtt ctgatttgaa agacattcca ttattattt
//
LOCUS       DBEJAQ010000083         4523 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.083, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000083 DBEJAQ010000000
VERSION     DBEJAQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.083"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(19..732)
                     /gene="rsmG"
                     /locus_tag="ACL297_002006"
     CDS             complement(19..732)
                     /gene="rsmG"
                     /locus_tag="ACL297_002006"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGR0072200.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            826..1386
                     /locus_tag="ACL297_002007"
     CDS             826..1386
                     /locus_tag="ACL297_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGR0072201.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            1388..2287
                     /gene="htpX"
                     /locus_tag="ACL297_002008"
     CDS             1388..2287
                     /gene="htpX"
                     /locus_tag="ACL297_002008"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HGR0072202.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(2436..3995)
                     /locus_tag="ACL297_002009"
     CDS             complement(2436..3995)
                     /locus_tag="ACL297_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGR0072203.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(4277..>4523)
                     /locus_tag="ACL297_002010"
     CDS             complement(4277..>4523)
                     /locus_tag="ACL297_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGR0072204.1"
                     /translation="NGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRRWLPK
                     GTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT         1262 a          859 c          946 g         1456 t
ORIGIN      
        1 atttgtttac taaaaaattt aaagtgggcg tttatttggc ataccagcct tacgtggata
       61 tttatttggt gtttcttttt tcttttctac cactgtgata tagcgcggat ctctattcgg
      121 tagggcgtag ctgagattgt cttcgacctt actaaaaagg agattgaggg cattcttagc
      181 ttctaacaac tcctcaggcg cattgctagc cttgagtgct aatagtttgc caccaacctt
      241 aaggtaggga atagtcaatt cagataggac ctgcatacgg gcaaccgcac gagctgttac
      301 aaaatcatat tgagcacgga agttcttgtc ttgggcaaaa tcttcggcac gtccgtggta
      361 gaaatgaact ccgttcaaat ccagttcttg agccaagagt tgtaggaagt tgatgcgctt
      421 attgagtgaa tcaataatgg tcacatctaa ctccggatag agaattttca ttggtagact
      481 aggaaatcct gccccagccc cgatatcaag aagtttgata gtttcattgg gaatcaaacc
      541 ttgaagaatg ggtgcaatcg aatcgtaaaa atgtttgaga taaacttctt ccttgtccgt
      601 aatcgccgtc aaattaatct tctcattcca ctcgaccaag agctcaaaat aacgttcaaa
      661 ttgttctttt tgctggtccg aaagtggaag attctgctcg gcaagcaagt tgtaaaatgt
      721 ttctggtttc atagttattt ccttctttag tatcatctta ttttaccaca atcattaaaa
      781 atttgatata ctggtactaa tctaaaagta gaaaggactt atatcatgac ttggattatt
      841 cttggagtta tcgctcttat tgttattttt gtgattgtta gctataacgg tttggttaaa
      901 aatcgtatgc aaaccaagga ggcttggagt cagattgatg ttcagttgaa acgtcgaaat
      961 gacctcttgc caaacttgat tgagactgta aaaggttatg ccaaatatga aggttctacc
     1021 cttgaaaagg tggcagaact acgtaaccaa gtggcggcag cgacttcacc agcagaagct
     1081 atgaaagcca gtgatgccct cactcgtcaa gtttcaggta tttttgcagt tgcagaaagc
     1141 tatccagatt tgaaagctag tgctaacttt gttaaattgc aagaggagtt gacaaacaca
     1201 gaaaataaaa tttcttactc tcgtcaactc tataacagtg ttgtcagcaa ctacaatgta
     1261 aaattagaaa ctttcccgag caatattatc gctggaatgt ttggatttaa agcggcagat
     1321 ttccttcaaa cacctgaaga ggaaaagtcg gttcctaaag ttgattttag cggtttaggt
     1381 gactaagatg ttgtttgatc aaattgcaag caataaacga aaaacctgga ttttgttgct
     1441 ggtatttttc ctactcttag ctcttgttgg ttatgcggtt ggttatctct ttataagatc
     1501 tggacttggt ggtttggtta tcgcactgat tatcggcttt atctacgctt tgtctatgat
     1561 ttttcaatcg acagagattg tcatgtccat gaatggagcg cgtgaggtgg atgagcaaac
     1621 ggcaccagac ctctaccatg tagtggaaga tatggctctg gtcgctcaga ttcctatgcc
     1681 ccgtgttttc atcattgatg atccagcctt aaatgccttt gcgacaggtt ctaatcctca
     1741 aaatgcggct gttgctgcga cttcaggtct actagctatc atgaatcgtg aagaactaga
     1801 agctgttatg ggacatgaag tcagtcatat tcgtaattat gatatccgta tttcgactat
     1861 tgcagttgcc cttgctagtg ctatcaccat gctttctagt atggcaggtc gtatgatgtg
     1921 gtggggtgga gcaggtcgca gacgaagtga tgatgaccga gatggaaatg gtcttgaaat
     1981 cattatgcta gtggtttccc tactagctat tgtactggca cctctcgctg caaccttggt
     2041 tcagctcgct atttctcgtc agagggaatt tctggcagat gcatctagtg tcgagctgac
     2101 tcgcaatccc cagggaatga ttaatgccct agataagttg gacaatagca aacctatgag
     2161 tcgccacgtc gatgatgcta gcagtgccct ttatatcaat gatcccaaga aaggtggggg
     2221 gttccaaaaa ctcttttata cccacccacc tatctcagaa cggattgaac gtttaaaaca
     2281 tatgtaaaat gaaggctgga aaaaagtctt taaaatctga aaaacgcata atatcaggtg
     2341 tgaaaacttg atattatgcg ttttactatg ggaagattta cttctttttc tcctaaaatt
     2401 gtgtttttgc cccacctatc tgctatgttg caaatttata aatcttctaa attaactagc
     2461 ttcaaaccgt gttctgtcat gcgatagatg atcgagcaga cttcttttaa gaaacgacgg
     2521 tcatgcgaaa cagtgatgag accgccagga taagtggcaa agagttttct gatttggggt
     2581 tgagaagtgg gagaaaagtt tcgtgtgggt tcatccagca ggagaaagtt tggtttgcgc
     2641 aggactaaat ccaaaagcag gagttttccc tgttgtccgc cagataagga gcgaatttga
     2701 tgctgcattt ctggataact gaaattgaga ctagctaggt gagattggat tttctgtagt
     2761 tcctcttttt ccccagtttt actgagatag gctattgggg ataaatccaa ttgcagtttt
     2821 ttgtggtaat cttgtggcat aaaaccaagt gaaatctctc ttttatcatt cagaagtctc
     2881 tgtaacttgg ctaacagagt tgatttccca acaccatttg gcccgataat accgattttt
     2941 tcttggccac ggacagttag ttgtagtttt tgaaccaaaa ctcggtcgtc aatggacaaa
     3001 ttttcttttt ccagttggac taagacttta gaagctggta atggttggat gtctgaaaag
     3061 aaaagttgga tttgttcctc ttcaagtggt ttttgagtca tggactgagc tgccttttcg
     3121 tagcgttttt cttgtgagag gacagttttc atctttttag ccaataggcg accggcagta
     3181 ctatctttgg tagctcgaag cgcagtttct acattttgct taactctccg atgtttttcc
     3241 atggttttat cgtaggctct ttggttgtta gcagcttgct gactttgttt ggcaaaatta
     3301 gcctttctct gctcactata gctatcatag tctaaatgct ctactagcgt ttccgcttct
     3361 ttacggtgtt tgaccagtcg caagtgaaca atagtgtctg ccgtttcaga aagaaagtct
     3421 tcatcatggg aaatgaaaat aacggtttgc ctggtctttt gaatctggcc ttttagccaa
     3481 tcaactgtct caaggtctag gtcatttgaa ggttcatcta aaaatagaat ctcaaagggt
     3541 ttggctaact catggataag ctgaattttc aaagcttctc cccctgatag attgccaatc
     3601 tcttggtcac ttgcgaaacg attgctatca aaatgcaatt cctccgccaa acgatagagg
     3661 atactgtagt ctaaatcaat agaatctaaa aagaagtagt cgtgtaaagt tttctttttc
     3721 agctcctcgg gaagtttttg aggaatgtag gccagtgact gatagtcaga ttggatgtct
     3781 cccttgatag tgaaatcaga caaatcttcc cccattaaag ttttaagtaa ggttgattta
     3841 ccatttcctt cttcgccaat aatagctacc ttttccccgt cttgtatggt catgcttagg
     3901 tcagagacaa ggtctcgtaa atctttgttt tgtgtgatgg tcaggtgatt gatttttatc
     3961 atactattgc cctttctaga atgtcgatag tgcataacaa aaagctctac tttacctagt
     4021 agagcccaaa gtctatgtca aaaaaactct attaacatgc cagaaaaaga gcataaaaga
     4081 gcacacaaaa agagacaaaa acaagatcta ctaaataaag gttctttatt tgatagactt
     4141 agttgttcat gtctccctta cctccgagta ttagtatctt tatattatac ctttaaggaa
     4201 attttgtcaa cagaaaactg gaaattctat gctctaaaat ccaaaaacag gtccataccc
     4261 aaatttcaag ttgaagttag ccacccaaaa gaaattcact tggcgacttg tagtccaagc
     4321 attttttagg gtagttgtta atccaatttt cgataaaagc tacttctttc ggagtcgttt
     4381 tcttggttcc tttaggtaac catctccgaa ttaatcgatt atgattttca tttgaacctc
     4441 tctcccatga agagtaagca tgtgcgtagt agatatgttc ctcaggaaat acctcagaca
     4501 atcgtttgaa ctccgaacca tta
//
LOCUS       DBEJAQ010000084         4449 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.084, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000084 DBEJAQ010000000
VERSION     DBEJAQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.084"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(19..926)
                     /locus_tag="ACL297_002011"
                     /pseudo
     CDS             complement(19..926)
                     /locus_tag="ACL297_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            1182..3815
                     /gene="polA"
                     /locus_tag="ACL297_002012"
     CDS             1182..3815
                     /gene="polA"
                     /locus_tag="ACL297_002012"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGR0072205.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3900..4337
                     /locus_tag="ACL297_002013"
     CDS             3900..4337
                     /locus_tag="ACL297_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGR0072206.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         1210 a          824 c         1073 g         1342 t
ORIGIN      
        1 ggatattatg gagccatttt acaacagtgc ctcaaattca gatactgaca tacctaattg
       61 ctggctggca acctcagaag tcagaagacc ttggcgtact atgtctagga aggcaaaaag
      121 tttcctttct gcccttcctt caactttccc acgctctagt ccttgttcaa ggccttcagc
      181 cctagcagtt tccaaggcat agtcctgtgc taacaagact tgttcttctc gcatatgtag
      241 ttgactaaac attttcctgt cctcctcgga ccagctcttg tagtccagca gttgatttgc
      301 ttggctaatg gctcgttgcg gttgctgggt aaagggcttg ttgccgaaaa actccaacca
      361 tggcttgcga accttgtctt tgctggtttc tctgtatttt ttaattccaa gaatgccatc
      421 ttaaccagat ggttttcctg tccattgttg gtaatcgcca atacctcacc tgttgtgtct
      481 tcgcgcatac taaagctatg aaaagccagg tcatctgaga aataattact atccacgata
      541 gcaatggcgt aaacaggagc gatgtgtttg tagctctagt gagtatcacc ttctcgctga
      601 cgaatttttt caagattttg attaacctga ctgcacaggt aagtccacaa gtgattgatg
      661 aaaaaattct gatgatggac ttgaatctca ataattactt gagtaccgtt atccaactcc
      721 gccaagacgt ctatactggt ataaaaatcc tgcaccgagt aaggcatgga gagtaatacg
      781 tgaatatcgc ttccctccaa aatggtcaca ttttttgctg gcaagtccag catatcgcga
      841 ataaattgac aagtgatttc tggattgctg aatattttct tagcaatcaa atcattagtt
      901 gggctgatgc ccggatgtct gagaatcctc ctattcctcc ttctatagtg agttgaaata
      961 agatgtgaat aactctatca ggaaagtcaa attaatttat agaaatattt tagcagtcaa
     1021 ggtgtactat tatagattca atatattata ttatagcaca tttttaaatc taggtttact
     1081 aaattctctg gctctatcta tttattcgga aaaagtttga aaaatacttg ctctagctct
     1141 tcccaatggt attttttgat tctttccttt ataatgggtg tatggataag aaaaaattat
     1201 tattgattga tgggtcttct gtagcttttc gggcgttttt tgcgctgtat cagcagttgg
     1261 accgttttaa gaatgcggct ggtttgcata ccaatgcgat ttatggtttt cagttgatgt
     1321 tgagtcattt gttggagcgg gttgagccga gtcatatttt ggtggctttt gatgcgggaa
     1381 agacgacctt ccggacagag atgtatgcgg actataaggg tggtcgggcc aagactcctg
     1441 atgagtttcg tgagcaattt cctttcattc gtgagttgct ggatcatatg gggattcgtc
     1501 actatgagct ggctcagtat gaggcggatg acatcattgg gacgctggat aagctagcag
     1561 agcaggatgg ttttgatatt accattgtca gtggggacaa ggatttgatt cagctgacgg
     1621 atgagcatac ggtggttgaa atttccaaga aaggtgtggc tgagtttgag gcctttacgc
     1681 cagattacct catggaagaa atgggcctca caccagctca gtttatcgat ctcaaggcgc
     1741 tcatgggtga taagtcggat aatatccctg gggtgaccaa agtcggtgaa aagacgggta
     1801 ttaagctctt gctggagtat ggttcgcttg aggggattta tgaaaatatt gatggaatga
     1861 agacttctaa gatgaaggaa aatctcatca atgacaagga acaggccttt ttgtctaaaa
     1921 cactagcgac cattgatacc aaggcaccga ttgcgattgg tttagaggac ttggtctata
     1981 gtggtccaga tgttgaaaat cttgggaaat tttacgatga gatgggattc aaacagctca
     2041 agcaggcttt aaatgtgtcg tcagctgatg tggctaaggg tttggatttt actattgttg
     2101 accaaatcag tcaagatatg ctgagtgaag agtctatctt ccactttgag ctttttggtg
     2161 agaattacca tacggataat ttggttggat ttgcctggtc ttgtgaggat aagctctatg
     2221 ccacagacaa gcttgagctg ttgcaagacc cgattttcaa ggatttctta gaaaaaacat
     2281 ctctgagagt ttatgacttt aagaaggtta aagttctttt gcaacgtttt ggtgtggatt
     2341 tgcaggcgcc tgcttttgac atccgtttgg ctaaatacct cctttcgact gtggaggaca
     2401 atgaaattgc gaccatcgct agtctttatg gtcagactta cttggttgat gatgaaactt
     2461 tctacggtaa gggtgttaaa aaggccattc ctgaacgtga gaaattcttg gaacacttag
     2521 cttgtaaact tgctgttttg gtagaaacag agcctatttt acttgaaaaa ctcagcgaaa
     2581 atgggcaatt agagcttctt tatgatatgg agcaacctct ggcttttgtt cttgccaaga
     2641 tggaaattgc tgggattgtg gtcaagaaag agaccttgct tgagatgcag gctgaaaatg
     2701 agcttgtcat tgaaaaactg actcaagaga tttacgagct ggctggtgag gagtttaatg
     2761 tcaactcgcc taagcagttg ggcgtgcttc tctttgagaa attgggactt cctctagaat
     2821 acactaagaa aaccaagaca ggttattcga cagcagtgga tgtgttggag cgtctcgctc
     2881 ctattgctcc gattgttaag aaaatcctgg attaccgtca aattgctaag attcaatcta
     2941 cttatgtaat tggcttgcag gactggattt tggctgatgg aaagattcat actcgctata
     3001 tgcaggattt gacccagacc gggcgtttgt ctagtgtgga tccaaacttg caaaatattc
     3061 ctgcccgatt ggaacaaggg cgcttgattc ggaaggcttt tgtgccagag tgggaggata
     3121 gtgtgctact cagctctgac tattcacaga ttgaattgcg cgttttggcg catatttcta
     3181 aggatgagca cttgattaag gccttccaag agggggcaga tatccatact tcgacagcca
     3241 tgcgggtctt tggcattgag cgtcctgata atgtgactgc aaatgaccgt cgcaatgcca
     3301 aggcagttaa ctttggagtg gtttatggga tttcagactt tggcttgtct aataatttgg
     3361 gaattagtcg taaggaagcc aaagcctaca ttgataccta ctttgaacgt tttccagata
     3421 ttaaaaacta catggatgaa gtggtgcggg aggcgcgtga taagggctat gtagagaccc
     3481 tctttaagcg tcgccgtgag ttgccagata tcaattcgcg caacttcaat attcgtggtt
     3541 ttgcggagcg aactgctatc aactcaccta tccagggttc ggcagcagat attctcaaga
     3601 ttgccatgat tcagctggat aaagccttgg ttgcaggtgg ttatcagact aagatgctgt
     3661 tacaagtgca cgatgaaatc gtccttgaag tgcctaaatc tgaattggta gagatgaaaa
     3721 aattggtgaa acaaaccatg gaagaagcca ttcaactcag tgttcctctt atcgcagatg
     3781 agaatgaagg ggcaacctgg tacgaggcta aataaaaagg gggctagtcc tccttttttg
     3841 tagtataatt ctgcaaacct tttcaaaatg tgctatactg atgaaaaagg aggatttcta
     3901 tgagtcaaga atttatcaat ccaagtgatg gcgtgattcg tcagtatctc gcaacgagta
     3961 aaacccttgc tgtggtgggt ttgtctgacc gtgaagaaac aaccagcaat cgagtaacaa
     4021 aggaaatgca ggctcggggc tataaaatca tcccagtcaa tcccaaggcg gcaggtggcg
     4081 aaatcttggg tgaaaaggct tatgctagcc tcgctgaaat tccttttcct gtagatattg
     4141 tcaatgttta tcgtcgcagt gaatttctgc ccgatgtggc gcgtgatttt ctcaaagctg
     4201 atgccaagat tttttgggca cagctaggac ttgaaagttt agaagcgaaa gagattttgc
     4261 gtgatggtgg atgtgatgat atcgtgatga atcgctgtat caagagagaa catactcgct
     4321 tgattgagga agcataagaa aaaggtagct gatgggctac cttttgtgtt atactcaatg
     4381 aaaatcaaag agcacactag caaactagga aactagccgc gggctgctca aaacaccgtt
     4441 ttgaggttg
//
LOCUS       DBEJAQ010000085         4425 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.085, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000085 DBEJAQ010000000
VERSION     DBEJAQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.085"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(58..1068)
                     /locus_tag="ACL297_002014"
     CDS             complement(58..1068)
                     /locus_tag="ACL297_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGR0072207.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1217..2386
                     /locus_tag="ACL297_002015"
     CDS             1217..2386
                     /locus_tag="ACL297_002015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGR0072208.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2383..3153
                     /gene="recO"
                     /locus_tag="ACL297_002016"
     CDS             2383..3153
                     /gene="recO"
                     /locus_tag="ACL297_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGR0072209.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3150..4142
                     /gene="plsX"
                     /locus_tag="ACL297_002017"
     CDS             3150..4142
                     /gene="plsX"
                     /locus_tag="ACL297_002017"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGR0072210.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4148..4381
                     /locus_tag="ACL297_002018"
     CDS             4148..4381
                     /locus_tag="ACL297_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGR0072211.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
BASE COUNT         1286 a          845 c         1135 g         1159 t
ORIGIN      
        1 aacatatacc tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtgtta
       61 gaaaatgccg ataagggtct gcataccaag agtggtgagg atgatggcaa tccagcagat
      121 ggctccaaga acaatggatt ttccactgga tttgaccata gcgaccagat tggttttgag
      181 accgatagca ctcatggcca tgacaataag gaatttagag agttgtttga gaggggtaaa
      241 gaaactactg gacacaccta gagaggtgag tagtgtagtg aggagagagg caaggataaa
      301 gtaaaggata aaaagtggga agactttttt cagttgtagg ctttgcttgt tttcttgttg
      361 gcgactttgc cagtaggata gaaagagcgt gatagggata atggccaaag tacgtgtgag
      421 tttaacaatg gttgcagact cgagggtatt gctttggtaa agactgtccc aagcgctggc
      481 ggcagccgtt acagaggaag tgtcgttgac cgcagtccct gcaaagaggg cgaagccttc
      541 attggataga tgaagccagg tgccgagggt tggaaagatg agcgcagcca agacattgaa
      601 gaaaaagata acggaaatgg cttgggctac ttccttttcc ttagcatgga taacaggcgc
      661 tgtcgctgca atggcagaac ccccacagat agaagaacct actccaacca aggtagccag
      721 ttttgtatcc agtgcaaaga aacgctggaa gaggtaggca ataatcagag ctattgagat
      781 agtggacagg atgacaggga gtgaagattg gccaactgcg aagacctgcg agatattgag
      841 accaaaacca agcaagacaa cggcatattg gagcaacttc ttggaactaa aggtcaaacc
      901 agcatccagt tgtttatagg acgagagaaa gggatgtagg agcatgccta tgaaaatggc
      961 aaaaacgggc gcgccaatca cagggaagaa tcctcctaag taccaagata ggatggaaat
     1021 gagaaggcag gccaagatac ctgctccatt ttttgataga aatgacataa aaacctccaa
     1081 ataaaatcta ttaccattat agacctataa acaggaaaag taaaatagaa agtggaaagc
     1141 tattctctaa tgtatttttg cggtcggggg gcttttgtag tataatagag atacgttttg
     1201 aaagtaggag gtatctatgg acttaactaa gcgctttaat aaacagttag ataaaattca
     1261 agtttcgttg attcgtcagt ttgaccaggt tatttcggag attcctgggg tcttgcgttt
     1321 gaccttgggg gaacctgatt ttacaacgcc agaccatgtc aaggaggcgg ccaagcgagc
     1381 gattgatcag aaccaatcct actatacagg gatgagtggt ctgctgactc tacgtcaggc
     1441 agccagtgac tttgttaagg aaaagtacca actggactat gctcctgaaa atgaaatctt
     1501 ggttacaatt ggggcgacag aggctttatc tgcgactttg acggctattt tggaagaggg
     1561 agacaaggta cttttgccag ctcctgctta tccaggctat gaaccgattg ttaacttagt
     1621 tggggcagaa attgttgaga ttgatacgac tgaaaatggt tttgtcttga ctcctgagat
     1681 gttggagaag gccattttgg agcagggtga taagctcaag gcggttattc tcaactatcc
     1741 agccaatccg acaggaatta cctacagtcg agagcagtta gaggccttgg cagctgtttt
     1801 acgcaagtac gaaatttttg ttgtctgtga tgaggtttac tcagaattga cctacacagg
     1861 cgaagcccat gtgtctctag gaacgatgtt gagagaccag gctattatta tcaatggttt
     1921 gtctaaatcg catgccatga caggttggcg tttggggctg attttcgctc ctgcgacctt
     1981 cacagcccag ttaatcaaga gtcaccagta cttggtcact gccgcaaata ccatggcgca
     2041 acatgctgca gtagaagcct tgacggctgg taaaaacgat gcggagccca tgaagaagga
     2101 atatatccaa cgtcgggact atatcatcga aaaaatgact gctcttggtt ttgagattat
     2161 caaaccagac ggtgccttct atatttttgc taaaattcca gcgggctaca atcaagactc
     2221 ctttgctttt ctgaaggatt ttgctcagaa gaaggccgtt gcctttatcc ctggtgcagc
     2281 ctttggacgt tacggggaag gctacgtccg cctatcttat gcagccagca tggagactat
     2341 caaagaagcc atgaaacgac ttgaggagta catgagagaa gcatgattca gtctatcacg
     2401 agtcaaggct tggtgcttta caatcgcaat tttcgtgagg atgacaagct cgtcaaaatt
     2461 tttacagagc aggttggcaa acgcatgttt tttgtcaaac acgctggtca gtctaagctg
     2521 gcgcctgtta ttcagccctt ggtgctggca cgatttctct tgcgaatcaa tgatgacgga
     2581 ctcagttaca tcgaagacta tcatgaggtc atgacttttc ccaagattaa tagtgacctc
     2641 tttgtcatgg cctatgcgac ctatgtggca gctcttgcag atgctagttt gcaggacaat
     2701 cagcaggatg ctcccttgtt tgcttttttg caaaagactt tggagttgat ggaagcaggc
     2761 ttggattatc aggttttgac caatattttt gaaattcaaa ttttgactcg atttggaatc
     2821 agcctcaatt ttaatgagtg tgtcttctgc catcgggttg gtcaggcttt tgacttttct
     2881 ttcaaatatg gagcctgcct ctgtccagag cattatcatg aggataagag acgttgtcat
     2941 ctcaatccca atatccccta tctgctcaat caatttcaag ctattgattt tgagactttg
     3001 gagaccattt cgctcaagcc tggaatcaag caagagctac gccaatttat ggatcaacta
     3061 tatgaagagt acgttgggat tcacctaaaa tcaaagaaat ttattgattc cctagcagac
     3121 tggggacaat tactaaaaga ggaaaagaaa tgaaaaaaat cgcagtagat gccatggggg
     3181 gcgattacgc acctcaggcc attgttgagg gtgtcaatca agccctatct gacttttcag
     3241 atatcgaggt tcaactttac ggagatgaag ctaaaatcaa gcaatatctg acagcgacag
     3301 agcgcgtcag cattatccat acggatgaga agattgattc ggatgatgaa cctacgagag
     3361 ctattaggaa taagaaaaat gccagtatgg tattggcagc caaggctgtc aaagatggtg
     3421 aagcagacgc tgtcctttcg gctgggaata caggtgcctt gttggcagca ggattcttca
     3481 tcgtgggtcg tatcaagaat atcgaccgtc ctggactcat gtctaccttg cctaccgttg
     3541 atggaaaagg ttttgacatg ctagaccttg gtgccaatgc agaaaataca gcccagcacc
     3601 tccatcaata tgcggttcta ggttccttct atgctaaaaa tgtccgtggc attgcgcaac
     3661 cacgcgttgg tttgctcaac aacggaacag agagtagcaa gggcgacccg cttcgtaagg
     3721 aaacttatga attactggcg gctgatgaaa gtttgaactt tatcggaaac gtggaagcgc
     3781 gtgatttgat gaatggcgtt gcagatgttg ttgtggcaga tggtttcacg ggaaacgctg
     3841 tgctcaaatc catcgaaggg acagctatgg gaatcatggg cttgctcaag acagctatta
     3901 caggtggtgg tcttcgagcg aaactaggtg ccctccttct caaggacagc ctcagtggtt
     3961 tgaaaaaaca gctcaattat tcagatgttg gtggagcggt cttgtttggt gttaaggcac
     4021 ctgttgtcaa gactcatggc tcaagcgatg ccaaggctgt ttatagtacg attcgtcaga
     4081 tccgtaccat gctagaaaca gacgtggttg cccagactgc gcgtgaattt tcaggagaat
     4141 aaaagagatg acagaaaaag aaatttttga ccgtattgtg accattatcc aagagcgaca
     4201 gggagaggac tttgtcgtga cagaatcctt gagtctgaaa gacgatttgg atgcggattc
     4261 tgttgacttg atggagttta tcttgactct ggaagatgaa tttagtatcg aaatcagcga
     4321 tgaagaaatt gaccaactcc aaaacgtagg agatgtggtt aaaatcattc aaggaaaata
     4381 gtaatcggag ttccaagtca acggaagtag atggttttta gaaat
//
LOCUS       DBEJAQ010000086         4120 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.086, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000086 DBEJAQ010000000
VERSION     DBEJAQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.086"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            185..1730
                     /locus_tag="ACL297_002019"
     rRNA            185..1730
                     /locus_tag="ACL297_002019"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1782..1854
                     /locus_tag="ACL297_002020"
     tRNA            1782..1854
                     /locus_tag="ACL297_002020"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1815..1817,aa:Ala,seq:tgc)
     gene            1979..>4120
                     /locus_tag="ACL297_002021"
     rRNA            1979..>4120
                     /locus_tag="ACL297_002021"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1179 a          824 c         1211 g          906 t
ORIGIN      
        1 gttgacaagt gaaagtggct gtgatatact aatatagttg tcgcttgaga gaagcaagtg
       61 acaaagacct ttgaaaactg aacaagacga accaatgtgc agggcgctac aacataagtt
      121 gtagtactga acaatgaaaa aacaataaat ctgtcagtga cagaaatgag taagaactca
      181 aacttttaat gagagtttga tcctggctca ggacgaacgc tggcggcgtg cctaatacat
      241 gcaagtagaa cgctgaagga ggagcttgct tctctggatg agttgcgaac gggtgagtaa
      301 cgcgtaggta acctgcctgg tagcggggga taactattgg aaacgatagc taataccgca
      361 taagagtaga tgttgcatga catttgctta aaaggtgcac ttgcatcact accagatgga
      421 cctgcgttgt attagctagt tggtggggta agggctcacc aaggcgacga tacatagccg
      481 acctgagagg gtgatcggcc acactgggac tgagacacgg cccagactcc tacgggaggc
      541 agcagtaggg aatcttcggc aatggacgga agtctgaccg agcaacgccg cgtgagtgaa
      601 gaaggttttc ggatcgtaaa gctctgttgt aagagaagaa cgagtgtgag agtggaaagt
      661 tcacactgtg acggtatctt accagaaagg gacggctaac tacgtgccag cagccgcggt
      721 aatacgtagg tcccgagcgt tgtccggatt tattgggcgt aaagcgagcg caggcggtta
      781 gataagtctg aagttaaagg ctgtggctta accatagtag gctttggaaa ctgtttaact
      841 tgagtgcaag aggggagagt ggaattccat gtgtagcggt gaaatgcgta gatatatgga
      901 ggaacaccgg tggcgaaagc ggctctctgg cttgtaactg acgctgaggc tcgaaagcgt
      961 ggggagcaaa caggattaga taccctggta gtccacgctg taaacgatga gtgctaggtg
     1021 ttagaccctt tccggggttt agtgccgtag ctaacgcatt aagcactccg cctggggagt
     1081 acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg
     1141 tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatccct ctgaccgctc
     1201 tagagataga gttttccttc gggacagagg tgacaggtgg tgcatggttg tcgtcagctc
     1261 gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccctattgt tagttgccat
     1321 catttagttg ggcactctag cgagactgcc ggtaataaac cggaggaagg tggggatgac
     1381 gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg ctggtacaac
     1441 gagtcgcaag ccggtgacgg caagctaatc tcttaaagcc agtctcagtt cggattgtag
     1501 gctgcaactc gcctacatga agtcggaatc gctagtaatc gcggatcagc acgccgcggt
     1561 gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt gtaacacccg
     1621 aagtcggtga ggtaaccgta aggagccagc cgcctaaggt gggatagatg attggggtga
     1681 agtcgtaaca aggtagccgt atcggaaggt gcggctggat cacctccttt ctaaggataa
     1741 ggaactgcgc attggtcttg tttagtcttg agaggtcttg tggggcctta gctcagctgg
     1801 gagagcgcct gctttgcacg caggaggtca gcggttcgat cccgctaggc tccattggtg
     1861 agagatcacc aagtaatgca cattgaaaat tgaatatcta tatcaaatag taacaagaaa
     1921 ataaaccgaa aacgctgtag tattaataag agtttatgac tgaaaggtca gaaaataagg
     1981 ttaagttaat aagggcgcac ggtggatgcc ttggcactag gagccgacga aggacgtgac
     2041 aaacgacgat atgccttggg tagctgtaag taagcgatga tccagggatt tccgaatggg
     2101 ggaacccaac aggtaatacc tgttacctac atctgttaag gatgtgagga ggaagacgca
     2161 gtgaactgaa acatctaagt agctgcagga agagaaagca aaagcgattg ccttagtagc
     2221 ggcgagcgaa acggcagaag ggcaaaccga agagtttact cttcggggtt gtaggactgc
     2281 aatgtggact caaagattat agaagaatga tttgggaaga tcagccaaag agagtaatag
     2341 cctcgtattt aaaatagtct ttgtacttag cagtatcctg agtacggcgg gacacgtgaa
     2401 atcccgtcgg aatctgggag gaccatctcc caaccctaaa tactccctag tgaccgatag
     2461 tgaaccagta ccgtgaggga aaggtgaaaa gcaccccggg aggggagtga aatagaacct
     2521 gaaaccgtgt gcctacaaca agttcgagcc cgttaatggg tgagagcgtg ccttttgtag
     2581 aatgaaccgg cgagttacgt tatgatgcga ggttaagttg aagagacgga gccgtaggga
     2641 aaccgagtct gaatagggcg ccttagtatc atgacgtaga cccgaaacca tgtgacctac
     2701 ccatgagcag gttgaaggtg cggtaagacg cactggagga ccgaaccagg gcacgttgaa
     2761 aagtgcttgg atgacttgtg ggtagcggag aaattccaaa cgaacttgga gatagctggt
     2821 tctctccgaa atagctttag ggctagcgtc gacattagag attcttggag gtagagcact
     2881 gtttgggtga ggggtccatc ccggattacc aatctcagat aaactccgaa tgccaatgaa
     2941 ttatggtcgg cagtcagact gcgagtgcta agatccgtag tcgaaaggga aacagcccag
     3001 accaccagct aaggtcccaa aataattgtt aagtggaaaa ggatgtgggg ttgcacagac
     3061 aactaggatg ttagcttaga agcagctatt cattcaaaga gtgcgtaata gctcactagt
     3121 cgagtgaccc tgcgccgaaa atgtaccggg gctaaaacaa tttaccgaag ctgtggatac
     3181 ctttataggt atggtaggag agcgttctat gtgtgatgaa ggtataccgt gaggagtgct
     3241 ggaacgcata gaagtgagaa tgccggtatg agtagcgaaa gacaggtgag aatcctgtcc
     3301 accgtaagac taaggtttcc aggggaaggc tcgtccgccc tgggttagtc gggacctaag
     3361 gagagaccga aaggtgtatc cgatggacaa caggttgata ttcctgtact agagtatgta
     3421 gtgatggagg gacgcagtag gctaactaaa gcagacgatt ggaagagtct gtctaagcag
     3481 tgaggtgtga attgagtcaa atgcttaatt ctataacatt gagctgtgat ggggagcgaa
     3541 gtttagtagc gaagttagtg acgtcacact gccaagaaaa gcttctagcg tttaaacata
     3601 ctctacccgt accgcaaacc gacacaggta gtcgaggcga gtagcctcag gtgagcgaga
     3661 gaactctcgt taaggaactc ggcaaaatga ccccgtaact tcgggagaag gggtgctgac
     3721 ttaaagtcag ccgcagtgaa taggcccaag caactgttta tcaaaaacac agctctctgc
     3781 taaatcgtaa gatgatgtat agggggtgac gcctgcccgg tgctggaagg ttaagaggag
     3841 tgcttagcgt aagcgaaggt atgaattgaa gccccagtaa acggcggccg taactataac
     3901 ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa aggcgtaatg
     3961 atttgggcac tgtctcaacg agagactcgg tgaaatttta gtacctgtga agatgcaggt
     4021 tacccgcgac aggacggaaa gaccccatgg agctttactg cagtttgata ttgagtgtct
     4081 gtaccacatg tacaggatag gtaggagtct aagagatcgg
//
LOCUS       DBEJAQ010000087         3389 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.087, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000087 DBEJAQ010000000
VERSION     DBEJAQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.087"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..242
                     /locus_tag="ACL297_002022"
     CDS             <1..242
                     /locus_tag="ACL297_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HGR0072212.1"
                     /translation="NSHLIYADYFEMKLQKLLKDDTKVFEKSTFKFVEGYKIYLTESK
                     ESGIKQMDNVIKYFEFIESKSIALYFQKRLNELID"
     gene            complement(600..1133)
                     /locus_tag="ACL297_002023"
     CDS             complement(600..1133)
                     /locus_tag="ACL297_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HGR0072213.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1222..1998)
                     /gene="recX"
                     /locus_tag="ACL297_002024"
     CDS             complement(1222..1998)
                     /gene="recX"
                     /locus_tag="ACL297_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGR0072214.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2036..>3389
                     /gene="rlmD"
                     /locus_tag="ACL297_002025"
     CDS             2036..>3389
                     /gene="rlmD"
                     /locus_tag="ACL297_002025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGR0072215.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1108 a          572 c          593 g         1116 t
ORIGIN      
        1 aaaattcaca tctaatatat gcagactact ttgaaatgaa attacaaaaa ttattaaaag
       61 atgacacaaa agtttttgaa aaatctacat tcaaatttgt agaaggatat aaaatatacc
      121 tgacagaatc taaagaatct ggaattaaac aaatggacaa tgtcataaaa tattttgagt
      181 ttattgaatc taaaagtatt gctttatatt ttcaaaaacg attaaatgag ctgatagatt
      241 aaatatcatt ttctctgtta agatattgtt tttaaaatat tgtactaaat gattgatgct
      301 atgtggaaat acaaaaaaat gtttttgata cgaagttgac ctgtattttt tatactaatc
      361 attttcgtat tttttgtatt aaacgatata agtttgttgt aaacttacta ggaataaaga
      421 cattaaaaaa taatagtata tctatttgtt ttatatattt tacgaattct gcataaatct
      481 ctttctagta atgtgttgta actctgctat aatggattta ttcctttttg tatttacaca
      541 atttatttta tactaccaaa aaaacaaggt caggattttg ttcctgacct ttgacaactt
      601 taccgattct ttagttctac atagcgcttg taccaaatgt ttacataggc ttctgagaaa
      661 ggaccacgtc cattgttaat ccaatcaaca agaattttga catgttcttt taaaatatag
      721 tccaagtcat cagaataatt cattttgcgt ttgtgacgct cgtattcttc aacgtccaag
      781 agacgttttt ccccatctgt aaaaatttta acatccaaat cgtaatcaat atacttcagt
      841 gcttcttcat ccagatagta ggggctagcc atattgcaat agtaagaagt tccattatca
      901 cgaatcatgg caatgatatt aaaccaatat ttcttgtgaa agtaaacaat agccggttct
      961 cgagtgaccc aacgacgacc atcactttcg gtaacaagtg tatgatcgtt gacaccaata
     1021 atggcgtttt ctgttgtttt tagtaccatg gtgtcccgcc aagttcggtg gagactccca
     1081 tcatgcttat aactttgaat tgtaataaag tcgccttctt ttggaagctt cataactaac
     1141 caactttcta caatttataa gtttatcatt tactattgta ccataaaatt acctaaaatc
     1201 tgtgaatttc acttggaaat attaaagata ttctctaaga gcgcttgcta tatccgaaaa
     1261 atcgtagccc tttcgtgcta aaacttgagt taaacgctgc ttcagttcgt atccttcata
     1321 ctttcgagca tacttagtat attgcttatc aagttccttg aagatgagtt cttgagtcgt
     1381 ttcttggtca acttggctat ctaagtcgtc aaaggcaatt ttagcatcag aataagagaa
     1441 gcccttgtta gtcaagttct ggataatctt atcttgcagg gcacgagctg gaagttttcc
     1501 ctcatatttt ttcaatagtt tattggctac acgttgagca acttcagaaa aatcaaattc
     1561 tttcaagttc tcttctatag tagattttga aatccctttt tgagatagtt tctgagtcag
     1621 cacataagga cccttgtctc ctgaaagttg attggtattg atgatagcat aagcgtactg
     1681 accatcatta atccacttat cttctttaag attagcaata acttgagaaa cgatgttttt
     1741 atcaatatcg tattttttca gatattctct gacttctttt tcagtgcgtg ctttaaagga
     1801 taagtggtag agagcgagat tcttaccata agaaaattga gcaaagtctt gaatctcttt
     1861 caattcctct tcgcttatca ccttatctct cgataacata aaacgaacaa ttgtatcttc
     1921 ggtgatatag catttgtcgc cattatcaag ctccatcagg tagagtcttt ttttcttttc
     1981 aagttttgtg attttcatag ctctattata actcaaaatg tgataagata ggggtatgaa
     2041 tctgaaagtg aaacaaaaaa taccattaaa aatcaagcgc atgggaatta acggtgaggg
     2101 aatcggcttt taccaaaaaa cattagtctt tgtaccagga gctctcaaag gcgaagatat
     2161 ctattgtcag attacttcta ttagacgcaa ctttgttgaa gcaaaattac tgaaggtcaa
     2221 caagaagtct aaatttcgaa ttgtgccatc ttgtactatt tataatgaat gcggaggctg
     2281 ccaaatcatg cacctgcatt atgataagca gctggagttc aagacggact tacttcatca
     2341 agcgctgaaa aaatttgctc ctgcaggata tgaaaattat gaaattcgtc caactattgg
     2401 aatgcaggaa ccaaaatatt acagagctaa gttacaattt cagactcgaa aatttaaaaa
     2461 tcaggtcaag gcgggcttat atgcacaaaa ctctcactat ttagtagagt tgaaagactg
     2521 cctggtacaa gataaggaaa cccaagtgat tgctaatcgc ttagcagaat tacttactta
     2581 tcaccagatt ccaatcacgg atgagagaaa agttctaggt gtccgtacta ttatggtccg
     2641 acgcgcgaga aagaccggac aggttcagat tattattgtt acaaaccgcc agcttaattt
     2701 aactcaattg gtaaaagagt tggttaaaga tttcccagaa gttgtgacag tagctgttaa
     2761 tacaaataca gctaaaacca gtgagatata tggtgaaaag acagagatta tctgggggca
     2821 agagagtatt caagaaggtg tactcaatta tgaattttca ctatcccctc gagcttttta
     2881 tcaactaaat cctgagcaaa cagaagtcct ctatagcgaa gcagtaaaag cgctggatgt
     2941 tgataaagaa gaccatttga ttgacgctta ttgtggagtt ggaacgattg gatttgcctt
     3001 tgcaaagaaa gttaaaacac tcagaggtat ggatattatt ccagaagcta ttgaagatgc
     3061 caagcgaaat gctaaaagaa tgggatttga caatactcat tatgaagctg gaacggcaga
     3121 agagattatt cctcgttggt acaaggaagg ctaccgagca gatgctttga ttgttgaccc
     3181 accacgtaca ggtctggatg ataagttatt agatactatt cttacttatg taccagaaaa
     3241 aatggtttat atttcttgta atgtttcgac cttggctcgt gatttggtac gcttagtaga
     3301 agtctatgat cttcattata tccagtcggt cgatatgttc ccacatacag ctcgaactga
     3361 agctgttgta aaattaataa aaaaagttt
//
LOCUS       DBEJAQ010000088         3334 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.088, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000088 DBEJAQ010000000
VERSION     DBEJAQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.088"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..96)
                     /locus_tag="ACL297_002026"
     CDS             complement(<1..96)
                     /locus_tag="ACL297_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGR0072216.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISR"
     gene            complement(282..893)
                     /gene="rpsD"
                     /locus_tag="ACL297_002027"
     CDS             complement(282..893)
                     /gene="rpsD"
                     /locus_tag="ACL297_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGR0072217.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1088..2140)
                     /locus_tag="ACL297_002028"
     CDS             complement(1088..2140)
                     /locus_tag="ACL297_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGR0072218.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2137..2835)
                     /locus_tag="ACL297_002029"
     CDS             complement(2137..2835)
                     /locus_tag="ACL297_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGR0072219.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGCETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(3002..>3334)
                     /locus_tag="ACL297_002030"
     CDS             complement(3002..>3334)
                     /locus_tag="ACL297_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGR0072220.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT          941 a          743 c          635 g         1015 t
ORIGIN      
        1 acgtgagatt tggaaaacgt gtgatgcttc tgttatacta cctgttcgct cacaataaga
       61 gagaactttt ttacgaaaat ctattgaata tgccataaaa agattatacc acattgtgta
      121 ctatttttgg ttcactttac tatatcactt tggactatat cggtctagtt atgtctaaac
      181 gattatagct ggatactaag agaaatcaaa aaagcactaa gggagaacgt cccaaagtgc
      241 ttaatatcaa ggcctcaaag cctatcttat ttaataaaat attacaacat tttgttgtag
      301 aattcaacga caagtgcttc gttgatttct gggttgattt cgtcgcgttc tggcaagcga
      361 gtcaatgaac cttccaactt ttcagcgtcg aatgatacga atgctggacg tccaagagta
      421 gcttctactg cttcaaggat tgctggaact ttcaatgatt tttcacgaac tgagatcact
      481 tgacctggag ttacgcggta tgatgggata tcaacgcgtt tcccgtcaac aaggatgtga
      541 ccgtggttta cgaattgacg agcttgacga cgagtagtcg cgagaccaag acggtaaaca
      601 acattatcca aacgacgttc caaaagaagc ataaagttga aacctaggat tccacctttg
      661 atttttgtag cttgtacgaa caagttacgg aattgttttt cacctacacc gtaagtgaaa
      721 cgaagttttt gtttttcagc caattgcaaa ccatattctg acaatttaga acggttgttt
      781 ggtccgtgtt gtcctggtac atagttacga cgtgccaatt ctttacctgt acctgtaagt
      841 gaaaggccaa ggcgacgagc ttgtttccaa gatggtcctg tataacgtga catatgaatg
      901 tcctcctgat ataaataata tttcggtgga aatagtcact tagaaagccc tgattcgtgc
      961 agatgccctt cgcctaaaca gccaaggtta cttgtcataa gacacctgtt gacgagcttc
     1021 atgctttcct gctgatattt cacactaaat tcattttacc atgaatagct ggatttgtaa
     1081 aggggtttta ggctttattt tcactaccag agaggttgag aacgagggta aaggtacttc
     1141 ctagaccgta ctggctgctg actgtgattt ccccacccaa ttgatgggcc aattcacgcg
     1201 caatcgcaag tcctaatcca tgaccacctg tcttcatgtt acgcgaagtt tcgacacgat
     1261 aaaggcgttt gaaaatattt tccaaatcct ctggggcaat accctgccct tcatcggtca
     1321 cactgattga aagctggtcc ttctccagct tagccaccac ttccagcttg gttcctggag
     1381 cagaatattt aaaagcgtta tcgaccagat tcaccaagat acgagaaagc ttagcataat
     1441 ctccctcaat ccgggcagac tctgggatta cctgcaagtg gacatctctt ctctcctgct
     1501 caatcaaaaa ctgaaattca ctcatgcact caattaagag cttgtccaga aaaatactgt
     1561 ctttactggt agtttccacc tgatttctag ctgtgtttag ggtcaaaaaa ttcaactcct
     1621 caaccagttt attgagcctc tccgtctggc gtccaatggt tgctagataa tgagcttgct
     1681 ccgactcctt gataatacca tccaaaatcc cttctaccgt cgcttggatc gaagtgatag
     1741 gagtcttaat atcatgcgac aactgggcaa tcatcaagcc cttttctcgt tcgctttctt
     1801 ccaaggaatc aaagcttacc tgcaaatcat gggacatctc attaaaagtt tgccctaatt
     1861 gctgaaattc tacaggacct tgaacctcca aatttgaagg aaaatccttg gccgctaccc
     1921 gcttggcatg ctccttgagt ttgcccaacg acgtaaagac tggcaatagg agaaagagac
     1981 taatcccagc accgacaagg ctggcaacga tggtcatccc aagcaaaaag taaatctcgc
     2041 ctttcgcaat cagcattttt tgaacagccc aaaaaacaac caaaatggtt aagagggttg
     2101 aaataatata tccaaccaaa atataacttt ttagtttcat gtttgtcctc tcggtttctc
     2161 tatcttatat cccaaccccc aaactgtctt aatagtgggc gtttggtcac tactatattt
     2221 tgccagctcc tgtcgaagag catggatatg cacattcaag gtattggtgt catccacgta
     2281 gtcttctttc cagatctttt catagaggtc tgtcttggag aaaactcgct ctggattgct
     2341 agctaaaatc cacagcaatt caaatgattt aacagttaaa tccagcattt cttctcctat
     2401 ttgaacttca tgactactat gattcatttt tagattgcca agggaaatca gctctgtttc
     2461 gcacccacga tgaaggcggc gcaaaatatt gtggacacgc aaaactagct cacgtgggct
     2521 aaaaggctta gcaataaaat catctgctcc caagctcagg ccgtaaatct tgtcctgttc
     2581 actggtctta gcagtaataa ataggaaagg ctgctctggt gataagtatt gaacctcact
     2641 gattaaatca taaccatcca tccgaggcat catgacatct gtgataatca aatcaatcgg
     2701 ttttttcttg aacagctcta gcgcttccag tccatcatgg gctaccaaga cctgataacc
     2761 tgcctgaatt aagtatctct gatgaatatc tgtgatttct acctcgtcgt caacgagtaa
     2821 aattgtcttt cccatctgtc tctcctttga taaaaacagt gctatactgc tttaagtata
     2881 acactatttt tattgtttta cgatgatttt aatctgctat tgaaagaaag tcctgattgc
     2941 tgattgagtt gttaaactaa tcttagtcag cccactatcc atcatttatt tccttagctg
     3001 attagttttc ttcttttttg tttttcaaac ctagaccacc gagtaaacct gcaagcgcaa
     3061 gcccaaggaa accaatactt gccattgatg tttgagtctc accagtattt ggtagcatag
     3121 ctttatcctc tgacatcatc gtatcagaca tcttgttagc agaagcagcc atgttttcac
     3181 ctgccatcgt gttggtagaa cttgtcatgg tgtcagcagg catgctatct gtagtacctg
     3241 tagcatgatt gtgattcatc ggagtcacgc cagaaccaga gttagaaggt gataatgaac
     3301 catttgctgt gtctgatgtt tctttaacat ttat
//
LOCUS       DBEJAQ010000089         3248 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.089, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000089 DBEJAQ010000000
VERSION     DBEJAQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.089"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..346)
                     /locus_tag="ACL297_002031"
     CDS             complement(<1..346)
                     /locus_tag="ACL297_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072221.1"
                     /translation="MKILSKKECESILGYLNFIGITLLFFVIISLSIYFKSYLPFQGK
                     LFLLFLVVDYATLNRYNMKLPIQSKKINTFYIRKNYIFLYLLAIVKKYYSFQILQSFI
                     YLFILYFLDIIGY"
     gene            complement(353..649)
                     /locus_tag="ACL297_002032"
     CDS             complement(353..649)
                     /locus_tag="ACL297_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HGR0072222.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(1192..2085)
                     /locus_tag="ACL297_002033"
     CDS             complement(1192..2085)
                     /locus_tag="ACL297_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0072223.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(2106..2300)
                     /locus_tag="ACL297_002034"
     CDS             complement(2106..2300)
                     /locus_tag="ACL297_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072224.1"
                     /translation="MKKVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVLPL
                     FSLIVLYMIDKRIRKKKKSL"
     gene            complement(2708..2833)
                     /locus_tag="ACL297_002035"
     CDS             complement(2708..2833)
                     /locus_tag="ACL297_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGR0072225.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            complement(2836..3066)
                     /gene="blpU"
                     /locus_tag="ACL297_002036"
     CDS             complement(2836..3066)
                     /gene="blpU"
                     /locus_tag="ACL297_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HGR0072226.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
BASE COUNT         1116 a          632 c          356 g         1144 t
ORIGIN      
        1 aatatccaat aatatctaga aaatacaaaa taaataaata aatgaatgat tgtaaaattt
       61 ggaaagaata atatttcttg acaatggcaa gaagatagag aaaaatatag tttttcctta
      121 tatagaaagt atttattttt ttactttgga taggcagttt catattataa cgattcaaag
      181 tagcgtaatc tactaccaaa aaaagtaaaa ataatttccc ttgaaaagga agatagcttt
      241 taaaatagat tgacaaactt atgataacaa aaaacaaaag ggttatccca ataaaattaa
      301 gataacctaa aatactttca cattcttttt tagaaagtat tttcatctag ttctaccata
      361 gagctgcttt agcagcacct ttctttttca atatataaag aactgttgct gcaatacccg
      421 aaccaataga acctactcct gtgattgcac ctacaattga aattactgct gtaactgtac
      481 tataagcact aatcaaattg actacagtag cagcagcttt tgtcgaaatt cctaatgtcc
      541 cagccaacat aggaaaagaa acaacaccta agcttaacca actaatgact gctaacattg
      601 ctgaaacaat aagaaattta ttatgaaatt ccattctttt agatttcatt ttagtgtact
      661 ccttttgtat attatgtatg ttttattgag agttaattcg cacatgttgt attcctaact
      721 ctcttcaaat gttcgtctat attttatact acttttatta acatatctat aataaataaa
      781 ttactactac attttcctag attatcaaat tacttttata tcactaagac tcaataaaag
      841 ataatgtcta tttaactttt atacgattac tacctccttt ttagtttaca cttcctacat
      901 cataaataaa catactcaac cctcacaatc tccaaataac tcttctatat cattgaaaat
      961 tgagtttcat cctctatact aatgtaggaa acctattaaa acattccttt aatccaatat
     1021 aagaatacct tgttccatta atctctaccc gatattatag atagaaccag actcctatac
     1081 ttaaaaggac aaataacgtt ccaaccataa taaacctcct ttctttttga gaacgttttc
     1141 tttataggtt tagtatagca aaaacatgaa aaaaacaacc tgacttagaa gtcaggttgt
     1201 tgggaaatct atctgttttt ctgctaaaat tctctgaact aatacctgat cgtctagcaa
     1261 ttctgctaga gaaatcgcct tttcatagaa actttctgcc tctaaaatat tcttgtctac
     1321 aaataaagca tattttgcct ttagcaaaaa tagatgaggt cggtaaaaaa catgtcttgt
     1381 tttagcaata attttctcaa atgtttgaat atatatcaga ctatcttctg tctgaatttg
     1441 gataaatagg ctcagaagaa ctttttccat ttgaactagg ctagccttat cttcatattc
     1501 tgaacatagc aatacttttt gagctaactc ctcaaaatgt tctttatctt ctaatcccat
     1561 agcacaacaa aagaaataca attcaataat caacaaatca tttacagaaa aatatttctt
     1621 ttttaataac tgctggaaat attcttccac taaccctaaa ccgtaggtca catctgaact
     1681 accataaata tcaaaacgag cttgtgtcac atccacaatt aactgctctt cctcaggcaa
     1741 attctcataa tagttttcat atatttcctc aagtatagct tcttttcgtt cgattcttcc
     1801 cttatcagcg taggtttgag aatgaatcag ctcatctttg agttttagga atccttttgg
     1861 aatctctata accttaacat ccacaatatt ctgtaatgat actccaagtt tatccgcaat
     1921 caataataat ttaggaagtg ttgctaaagc ttgcccattt tcaatacgtg ctagctgacg
     1981 aatcgtcaac tctgattcat caccacaaat atctatctga gtttttccac aagatcgcct
     2041 aatagttttg attttataac caatttcacg tttcaactca tccatagtaa tactccttaa
     2101 gttgattaca aagatttctt tttcttccta attcttttgt caatcatata taaaacgata
     2161 agcgaaaaca gcggtagaac aatataactc ttttcactca ttatcagctg aaatcccagg
     2221 gttgctagta tcttaaagat gaaaatattc atcaaaaata gaaaaacaaa tactaaaaaa
     2281 tattttaaaa ccttcttcat ttcttttctt caactttcct aatttctaca cctattttat
     2341 cgaaaatata tataaaactc caaataaaca cttcgactgc actttttttg gcaaaaaata
     2401 gacagaacaa acatcctatc tactaaaaat atgatatgaa aggaaattga ttttttactg
     2461 tcaccctagg caagcttgaa acaaataaat cattgatagt aacacttacg acatcctatt
     2521 tattacttca atttctaatg tctctgctat ttctaagcta cctctggtat gatctattcc
     2581 tagtcagaat agataacacc gttctaaatt acgattcata gttactcctt ttttgactcg
     2641 ttactataag actcctcttt acatgcgcaa aataattttg attcagattc atcatttata
     2701 gttttgatta ttttttaaca gatttttctg ttttcttctc cgaagacttt aaaaattttt
     2761 ttctaattaa atcatccata taaggaccaa atataccaac tactaaacca ataataaaac
     2821 ttttaaaatc cataattacc accaacatgt cgctgcatag gccacacctc caagtatagc
     2881 tcctcccgca gcaccagttg ctgcaccttg ccatgttctt gttttaattc ctagctgaag
     2941 acctcgtact gctcctcctc caacacctgc tttggcaaaa tctccccaat tgcatccgcc
     3001 accttcaacg caagcaagca tctcagtatc cataatttca aattgtgaca tcttttttgt
     3061 attcataacg aatacctctt ttttattttt aatatttgtc ttgttacaaa cttgacaagt
     3121 ttagtataac agtatctatt aatttttttc atccaaatct tgaattggca tcgaaacgtc
     3181 ttgaattagc ttttttgttt caaaatcatc tctattttta aaaaagatgt tttctaatca
     3241 ctttttta
//
LOCUS       DBEJAQ010000090         2559 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.090, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000090 DBEJAQ010000000
VERSION     DBEJAQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.090"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(214..879)
                     /locus_tag="ACL297_002037"
     CDS             complement(214..879)
                     /locus_tag="ACL297_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGR0072227.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(893..2272)
                     /locus_tag="ACL297_002038"
     CDS             complement(893..2272)
                     /locus_tag="ACL297_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGR0072228.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
BASE COUNT          838 a          581 c          477 g          663 t
ORIGIN      
        1 cgtcagttct atccacaacc tcaaaacagt gttttgagca gcccgcagct agtttcctag
       61 tttgatcttt gattttcatt gagtattact ctctcttgtc acttccttct attttaccat
      121 aaagtccagc ctttgaagaa cttttactag aagacaaggg gcttctgtct ctatttgcca
      181 tcttaagcat caaaaaagag gggtcatccc tctttacgaa ttcaatgcta ctagggtatc
      241 caaatactgg ttgttgatga ctgccaaaat ataggtatct gctttcaaga ggtcatctgg
      301 tccaaattca acatccaatg gggaattttc ctgctctcgg aaacccaaaa tattcagatt
      361 gtatttgcca cggaggtcta atttactcag actttgacct gcccaagact gaggaatttt
      421 catctccacg atagacacat ttttatccaa ctgaaagaca tcaacactat tatggaaaag
      481 aatggtctgt gctagagact gccccatttc atactctggc gagataaccg agtcagctcc
      541 aatcttttct agcactttct tagcggtctg acttttgacc ttagcaataa cagtcggtac
      601 ccccaaactc ttacagtgca taaccgcaag cacactcgac tccagatttt cacctgtcgc
      661 gactacaacg gtatcgcagg tatcaatccc tgctgatctc aataattctt catctgtgat
      721 gtcaccaatc actccacgcg ccaaaactgg ctcaaactga ttgatgcgct ctgcgtggtc
      781 atcaatagcg ataatattca tatcctgctt ggctagggca gctaggacac tgctcccaaa
      841 aattcccaag cccaaaattc caatcgtacg atctgacatg ctctttcctt tcttaaccaa
      901 tactaatatc tgctttcata tagtgaatca tatctttctt ttctggatgg taatctgcca
      961 agctaacaaa caaggtcaag ggaccaattc gtcccataaa cataagtggc atgataacac
     1021 tgagagccaa tttcccaagg tcaggagtca gatttgccgt tacaccaact gtactaagag
     1081 ctgaaatggt ttcaaatatg aggtggataa agggaggatt gcctttggct gttatcccta
     1141 gcagaatcaa tcctatcaag aagctcatca aaaagataat aaagacacta aaggattttt
     1201 gaaccgttcg cggcgcgatc gttcgtctcg caacattggc atgaggcaag cctagaagct
     1261 cacttcgtgc aaagaccaag aggacaaaaa atgtcgtaat cttgagtccc ccagctgttc
     1321 ctccaggtgc cccacctaga aacatctgta agatataaat caaaagagtc acaggatgag
     1381 cctgagtata atctatcgta gaaaagccag ctgttcgcat cgtcactgtt tgaaaaaagc
     1441 taactaaaac cttatcggca acagggagat tgccaatcgt tccagcattg ttccactcaa
     1501 gaaagagagt agttgccgtt ccaaataaca acaaacctat agtcaataat aatacaagct
     1561 tcgtatgaaa gtgcagacgt ccttttttct ttcttcctac atgaccagcc aaatcaaacc
     1621 agaccataaa accaaggccg cctgtaataa tcaagcctgc aatcaccaga ttgaccagta
     1681 aatcggtctg aaaagcaaat aaactggtgc tccctaaatt atcaaaaccg gcattacaga
     1741 aggctgagat cgctagaaaa atggaactaa aaagaccacg tccccagcca agttgaggaa
     1801 taaggcgaaa actaagcaaa atagctccca agctctcaac caaaaaggtc gtgagaaaaa
     1861 tagaatagac aaactttctc aaagatcgag tttctccata actaaaacta tcctgaatag
     1921 ttgcacggct acgaagacta agcttttgct tgctctggat atagaaaacc ccaataaagg
     1981 tcatgagccc tagaccaccg atctgaatca agagcaaaca gattatttgg ccccagatat
     2041 tataggtgtg agctactgga agggttgaga gacctgtcac acagactgca gagacagcag
     2101 tgaaaagatg atcaaaataa gtcgctcgtg agctttcaac ttggacaaag ggcaagctca
     2161 aaagaagaga gcctagtaaa atgaccaagg caaaactcaa aaaaatacga cgggctggcg
     2221 ataaacgaga taagcctagc tctatttttt ccttagataa tttgaataac atgtttccat
     2281 tgtaccataa aaagctcgaa atgacttaga taggataagg caaaattcac taacaccaag
     2341 cctaaaaaat gccacctttt tattgatagc ttaggcaaga acaatacttg atagatatga
     2401 ctataggtca aaaaagcaag acaggagctt ttaacgccag ctactctata tgcttttcag
     2461 gagaaaattt ctctttttga ttataggcaa tgaaaaccaa ggcatcgatt agaaagctag
     2521 tcacaggttg ctcaaaacac tgttttgagg ttgtggata
//
LOCUS       DBEJAQ010000091         2554 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.091, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000091 DBEJAQ010000000
VERSION     DBEJAQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.091"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(8..1318)
                     /locus_tag="ACL297_002039"
     CDS             complement(8..1318)
                     /locus_tag="ACL297_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGR0072229.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1555..2427
                     /gene="nadC"
                     /locus_tag="ACL297_002040"
     CDS             1555..2427
                     /gene="nadC"
                     /locus_tag="ACL297_002040"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGR0072230.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          784 a          436 c          614 g          720 t
ORIGIN      
        1 aataaatcta acttaaggaa gctagaagga tgagaatcca ggtggtcaag agtcccaaac
       61 ttaagctgat ggggacaccc agaataattt gctttttgaa ggcaaggcca cgttcctcta
      121 tattgggaag tgagagttga atgagagaac cagctgatga aaagggtgag atattagtag
      181 atagagcgcc aataacggtg gctgttgtga gtaagtgaat atcaatctga ggattttgag
      241 cactgatgat agcaatgatg ggaaagaggg ctggagctac aacggatagg gtggaactaa
      301 agagtgacat cactccggct atcacacaaa agaacagagg taaccagaaa tgaggaatgg
      361 ttgttgtcat gaggtgccct atcagtgtga ctaaacctga cttgaccgct agagacatta
      421 gtaagctcat gccgcagagc atgataattg tagcccaggg aaccttagct aaaatggctt
      481 cttgcttccc taatttgagc cttaaggcga ggcagaccat gagtattgag acaaagccaa
      541 tatcaaatgt tttttgataa gtagctatcc aggcgatgtt tgggaaaatg agatgcaaca
      601 agggaaaaag ccaaaccaaa accatgctgc tgatcatgag caaggtggtt tgtctttgaa
      661 ccttgctgag gagtggtggt tggtcaatag tcaaggatga gtttgttctt cccttactat
      721 agtgactgta acaggataat aaaagcaaga cgatgagtgg gtagataatg ctgacgataa
      781 agatatgatt gccaagtgaa aaagcttgct cttcccatcc catttgctta aacaggcctt
      841 gaaagacaat gcctgagcta ctggttatca aattagcccc tcctgaagct ccccaattga
      901 cggcttgagc tccaatcaaa gggtgtttgt ccgctttttg acagagggta atcgctagag
      961 gacagcaaac ggccatagta gtgaaaaatc cagcacctaa agcagacaaa agggttgcca
     1021 tcaggtataa aatcatgtag agggcgttag ggtgggtgcg tgtgcggtag agaatgtgtt
     1081 gagccaaaac atcaagagta ccgttagttg ttgcaacgtt ataaaagaga gagacgctaa
     1141 aaatggtaaa aaagagtgag gttggccaaa aatgaagaag ttctttgggg cttaatccca
     1201 tgagagtggt tgcgatgagg taagaaaaag caatagccag caggccaata ttgattttgg
     1261 tgcggtaacc aattccaatg gctagagcaa tggcgctaat cattattaaa tgaatcattg
     1321 aattgtcctt tcagttagaa tatggaaata ggttggcaat tttggcgctc aaaagaccac
     1381 ctgtttccta ttggtacttg actattatac acaaaaaaaa tacaattgtc ttgacaattg
     1441 tattgacact tgttagaatt aaaaatgagg tattcaggct tgtgatttcc tatggaagtt
     1501 aatagtaatt gcctctagtg cttacaagtg atattaaaaa tagaggactt agtgatgtca
     1561 atcatttcaa ctgatttaac cccttttcaa atagatgata cattgaaagc agccttgcga
     1621 gaagatgttc attccgaaga ttacagtacc aatgccattt ttgatcatca tggccaagcc
     1681 aaggtgtcgc tttttgccaa ggaagctggt gttttagcgg gactaaccgt ttttcaaagg
     1741 gtttttaccc tatttgatgc cgaggtgacc ttccagaatc ctcatcaatt taaggatggg
     1801 gatcgtttga ctagtggcga tttggtttta gaaatcatag gctcggtgag aagtctctta
     1861 acatgtgaac gcgttgcctt gaatttttta caacatttat cagggattgc ttcgatgaca
     1921 gctgcttatg tagaagcctt aggcgatgat tgcattaagg tatttgatac tcgaaaaact
     1981 actcctaatt tacgtctttt tgagaaatat gccgtgagag ttggcggtgg ctataatcat
     2041 cgctttaatt tatcagatgc tatcatgcta aaagacaatc acattgcggc agtaggtagt
     2101 gttcagaagg caattgctca agcgcgtgcc tatgcccctt ttgtgaaaat ggtcgaggtg
     2161 gaagtggaaa gccttgctgc tgccgaagaa gctgcggcgg cgggtgctga tattatcatg
     2221 ttggataata tgtcattgga acagattgaa caggccatta ccctaattgc aggacgttct
     2281 cggattgaat gttctggaaa tattgatatg accactatta gccgttttcg tggtttagcg
     2341 attgattacg tctccagtgg tagtttaacc catagtgcta agagtcttga tttttccatg
     2401 aagggtttaa cctaccttga tgtctaagtt gtaaaataaa ctaacttttt aaaggatgtc
     2461 tttcctctag aacgagtttt atgtcagata gtttaaacgc ctcttcaaat atagtaaaat
     2521 gaaccaaaaa tagtacacaa tgtggtataa tctt
//
LOCUS       DBEJAQ010000092         2535 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.092, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000092 DBEJAQ010000000
VERSION     DBEJAQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.092"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..559
                     /locus_tag="ACL297_002041"
     CDS             <1..559
                     /locus_tag="ACL297_002041"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGR0072231.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="ACL297_002042"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="ACL297_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HGR0072232.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKAGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1424..2029
                     /locus_tag="ACL297_002043"
     CDS             1424..2029
                     /locus_tag="ACL297_002043"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGR0072233.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1977..>2535)
                     /locus_tag="ACL297_002044"
     CDS             complement(1977..>2535)
                     /locus_tag="ACL297_002044"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGR0072234.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT          867 a          365 c          435 g          868 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcgggag ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaacca tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gctggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1501 aaaaataaaa gatatttttt caatgaatac aggtctttct tacaagaagg gcgatttaag
     1561 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1621 tctgttggat aatgattact acattgatac acaattcatc tcctctgaac aagtttattt
     1681 aaaacataat cagctaataa cacctgtatc aacctcttta gaacatattg gaaagtttgc
     1741 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1801 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtcct ctccgttatt
     1861 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
     1921 aactacacta agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     1981 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tggaaatgat tcttttcatc
     2041 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2101 ccttttatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
     2161 taatctctga taatattttc tttattagca taggggaata tcgatataat ggcttcatta
     2221 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
     2281 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
     2341 tctctcgtat tagttacata accagatata ggcatatctg atatagatac ccaaggtatt
     2401 tcagttcccc aaaaagtagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
     2461 aggctagcaa atttaatata tctccatgct tctgggattt catatatagg ataagaggtt
     2521 gtttcgtctt tgttc
//
LOCUS       DBEJAQ010000093         2302 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.093, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000093 DBEJAQ010000000
VERSION     DBEJAQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.093"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..1545
                     /gene="phtA"
                     /locus_tag="ACL297_002045"
     CDS             <1..1545
                     /gene="phtA"
                     /locus_tag="ACL297_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HGR0072235.1"
                     /translation="HVESDGLVFDPAQITSRTARGVAVPHGDHYHFIPYSQMSELEER
                     IARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSNSSLVSQLVRKVG
                     EGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQEFYDK
                     AYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPITHPER
                     LGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSHWIGK
                     DSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLVRLPY
                     MVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVEHPDE
                     RPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVEAQLK
                     EAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSNPSSV
                     SKEKIN"
     gene            1703..>2302
                     /locus_tag="ACL297_002046"
     CDS             1703..>2302
                     /locus_tag="ACL297_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGR0072236.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDT"
BASE COUNT          816 a          413 c          466 g          607 t
ORIGIN      
        1 catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg aacagctaga
       61 ggtgttgcag tgccacacgg agatcattac cacttcatcc cttactctca aatgtctgaa
      121 ttggaagaac gaatcgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca
      181 gattcaaggc cagaacaacc aagtccacaa ccgactccgg aacctagtcc aggcccgcaa
      241 cctgcaccaa atcttaaaat agactcaaat tcttctttgg ttagtcagct ggtacgaaaa
      301 gttggggaag gatatgtatt cgaagaaaag ggcatctctc gttatgtctt tgcgaaagat
      361 ttaccatctg aaactgttaa aaatcttgaa agtaagttat caaaacaaga gagtgtttca
      421 cacactttaa ctgctaaaaa agaaaatgtt gctcctcgtg accaagaatt ttatgataaa
      481 gcatataatc tgttaactga ggctcataaa gccttgtttg aaaataaggg tcgtaattct
      541 gatttccaag ccttagacaa attattagaa cgcttgaatg atgaatcgac taataaagaa
      601 aaattggtag atgatttatt ggcattccta gcaccaatta cccatccaga gcgacttggc
      661 aagccaaatt ctcaaattga gtatactgaa gacgaagttc gtattgctca attagctgat
      721 aagtatacaa cgtcagatgg ttacattttt gatgaacatg atataatcag tgatgaagga
      781 gatgcatatg taacgcctca tatgggccat agtcactgga ttggaaaaga tagcctttct
      841 gataaggaaa aagttgcagc tcaagcctat actaaagaaa aaggtatcct acctccatct
      901 ccagacgcag atgttaaagc aaatccaact ggagatagtg cagcagctat ttacaatcgt
      961 gtgaaagggg aaaaacgaat tccactcgtt cgacttccat atatggttga gcatacagtt
     1021 gaggttaaaa acggtaattt gattattcct cataaggatc attaccataa tattaaattt
     1081 gcttggtttg atgatcacac atacaaagct ccaaatggct ataccttgga agatttgttt
     1141 gcgacgatta agtactacgt agaacaccct gacgaacgtc cacattctaa tgatggatgg
     1201 ggcaatgcca gtgagcatgt gttaggcaag aaagaccaca gtgaagatcc aaataagaac
     1261 ttcaaagcgg atgaagagcc agtagaggaa acacctgctg agccagaagt ccctcaagta
     1321 gagactgaaa aagtagaagc ccaactcaaa gaagcagaag ttttgcttgc gaaagtaacg
     1381 gattctagtc tgaaagccaa tgcaacagaa actctagctg gtttacgaaa taatttgact
     1441 cttcaaatta tggataacaa tagtatcatg gcagaagcag aaaaattact tgcgttgtta
     1501 aaaggaagta atccttcatc tgtaagtaag gaaaaaataa actaatgaaa aatgaaagtc
     1561 tcgataaaga ggctttcatt tttattatgt atatatgtaa aattcttgac aagcaatatt
     1621 aaaaagagta aactattaac tagttaatta accggtttat tactttatag tgaatcaaat
     1681 atacttaaga aaagaggaaa gaatgaaaat caataaaaaa tatctagctg ggtcagtagc
     1741 tacacttgtt ttaagtgtct gtgcttatga actaggtttg catcaagctc aaactgtaaa
     1801 agaaaataat cgtgtttcct atatagatgg aaaacaagcg acgcaaaaaa cggagaattt
     1861 gactcctgat gaggttagca agcgtgaagg aatcaatgcc gaacaaatcg tcatcaagat
     1921 aacagaccaa ggctatgtca cttcacatgg ggaccactat cattattaca atggtaaggt
     1981 tccttatgac gctatcatca gtgaagaatt gctgatgaag gatccaaact accagctaaa
     2041 ggacgaggat attatcagtg aaatcaaggg tggttatgtg attaaggtag atggaaaata
     2101 ctatgtttac cttaaggatg cagctcatgc ggataatgtc cgtacaaaag aagaaatcaa
     2161 tcggcaaaaa caagaacata gtcagcatcg tgaaggaggg acttcaacaa acgatggtgc
     2221 ggtagccttt gcacgttcac aaggacgcta tactacagat gatggttata tctttaatgc
     2281 ttctgatatc attgaggaca cg
//
LOCUS       DBEJAQ010000094         2280 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.094, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000094 DBEJAQ010000000
VERSION     DBEJAQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.094"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            189..>2280
                     /locus_tag="ACL297_002047"
     CDS             189..>2280
                     /locus_tag="ACL297_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HGR0072237.1"
                     /translation="MTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNA
                     TKDKASKSELTQTAEELASRIASVHLGRRNLLKGTKELARYKPVSEYNGFKVIRTVAG
                     ATRYQDSYVERTVIPTAGTEYIAIFYARASENDYPVRCHFYNPNTVVSSENSSGYKSR
                     SSDGLSIIRLSTDWQLCWVKWTQTATDQAKTVIIGRHGPQVGGKEGVWVEICAPAIFE
                     GNLAGDWSPAYEDQDERVSAVESNFKQRADSLEAGVSRLTEGLRTKADISSLNVTAEN
                     IRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKASKSELTQTAEELSS
                     KIASVQVGGRNYIRGTKRMMLARGLWASGTFRPSGAGTAKTIDVSDSPATGFDKAIRL
                     TSSNARDQIGIAQDGFYISQGTYTMSCWVKGRRGQKVKLQTYWQVNDNSGISPIFTLK
                     DENWTKLSFTSARNRAGVASIGYVYLVNAEVGEYLDVLAPQLEDGSLATSSKEAPEDI
                     EGQISTVESTFKQRADSLEAGVSRLTEGLRTKADISALNVTAENIRQSVKSLETDTQN
                     KLNQKLSQAEFEVRAGSIRQEILNATKDKASKSELTQTAEELASRIASVQASGRNLFL
                     NSLFKQDIPKTGIWTTSTYTATIDSESKYLGHKALKIIGLNPSGRDGGNPKVTYPALG
                     QFGKVIPGSTTNQDV"
BASE COUNT          709 a          466 c          582 g          523 t
ORIGIN      
        1 tttggatttg gatgccgaag tttgaaataa gcgatgtaga tacttcttat tcagaagctc
       61 ctgaagatat agaaggtcag atttcaacag ttgaatcgac ctttaaacaa cgagccaact
      121 cgctcgacgc tggtgtaaga agcttgactg aagggcttag aaccaaagta gatatcagcg
      181 cactcaatgt gactgctgaa aatattaggc aatctgtgaa gagccttgag acagacacgc
      241 agaacaaact aaatcagaag ttgagtcagg ctgaatttga ggtgcgagcc ggctctatcc
      301 gtcaggaaat cctgaatgca accaaagata aagccagcaa gtcagaactc acgcagacag
      361 ctgaggagct agctagtcgg attgcgagtg tgcacttagg gcgcagaaat ctgctgaaag
      421 gcacaaaaga gcttgcgaga tacaagccgg ttagtgaata taatggtttt aaagttatca
      481 gaacagtcgc aggagcaact agatatcagg atagctatgt ggaaagaacc gttataccaa
      541 cggctgggac agagtatata gctatctttt atgcacgagc cagtgaaaat gactatcctg
      601 tgcgctgtca tttttacaat cctaacacgg ttgtatcatc agaaaacagc agcggatata
      661 agtcaaggtc gtcagatggc ttgtctatta tccgtctctc gacagactgg cagttgtgct
      721 gggttaaatg gacccaaacc gcaacagatc aagccaagac ggtcatcatt ggccgccatg
      781 gccctcaagt aggcggtaaa gagggggtat gggttgaaat ctgcgcccct gccatttttg
      841 agggaaatct tgcaggtgac tggtcaccag catacgaaga ccaagacgaa cgtgtctcag
      901 cggtcgaatc caactttaaa cagcgtgctg attcactcga agctggtgta agccgtctga
      961 ctgaaggcct tagaaccaaa gccgatatca gctcactcaa cgtaactgct gagaatatcc
     1021 ggcaatctgt gaagagtctt gagacagaca cgcagaacaa gctaaatcag aagttgagtc
     1081 aggctgaatt tgaggtgcga gccggctcta tccgtcagga aatcctgaat gcaaccaagg
     1141 ataaagccag caagtcagaa ctcacgcaga cagctgagga gctctctagt aagatagcga
     1201 gtgtgcaggt cgggggtagg aattatatcc ggggtacaaa gcgcatgatg ctagccagag
     1261 gattgtgggc atcaggtact tttagaccgt caggcgctgg gacggcaaag acgattgatg
     1321 tatcagacag tccagcaact ggctttgata aagcgatacg attgacctca agcaatgcta
     1381 gagaccaaat aggtattgct caagatggat tttatatctc gcaaggcaca tacacgatgt
     1441 cttgttgggt caaaggcaga agaggtcaaa aggtcaagct acaaacttat tggcaagtca
     1501 atgataattc gggtatttcg cccatcttta cattaaagga tgaaaattgg acaaagctat
     1561 cgtttactag cgctagaaat agggctggag tcgcatcaat tggctatgtg tatctcgtaa
     1621 atgctgaggt cggagaatat ttagatgttc ttgcgcccca gctggaagac ggaagtttgg
     1681 caacaagctc aaaagaagct cctgaagata tagaaggtca gatttcaaca gttgaatcga
     1741 ccttcaaaca gcgtgctgat tcactcgaag ctggtgtaag ccgtctgact gaagggctta
     1801 gaactaaagc ggatatcagc gcactcaacg tgactgctga aaatattcgg caatctgtga
     1861 agagtcttga gacagatacg cagaacaaac taaatcagaa gttgagtcag gctgaatttg
     1921 aggtgcgagc tggctctatc cgtcaggaaa tcctgaacgc aaccaaggat aaagccagca
     1981 agtcagaact cacgcagaca gctgaggagc tggctagtcg gattgcgagt gtgcaggcat
     2041 ccggtcgaaa tctattcttg aactcactat tcaagcagga tattccaaaa acaggaattt
     2101 ggacaacgag tacatatacg gctactatcg atagcgaaag taagtatctt ggacacaagg
     2161 ctcttaaaat tataggtttg aatccatctg gccgtgatgg aggtaatccc aaggttactt
     2221 atccagctct gggtcaattc gggaaagtaa ttcccggaag tacgactaat caagatgtaa
//
LOCUS       DBEJAQ010000095         2181 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.095, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000095 DBEJAQ010000000
VERSION     DBEJAQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.095"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            85..624
                     /locus_tag="ACL297_002048"
     CDS             85..624
                     /locus_tag="ACL297_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGR0072238.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQEFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            691..1101
                     /locus_tag="ACL297_002049"
     CDS             691..1101
                     /locus_tag="ACL297_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HGR0072239.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLY
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1217..>2181
                     /gene="pavB"
                     /locus_tag="ACL297_002050"
     CDS             1217..>2181
                     /gene="pavB"
                     /locus_tag="ACL297_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HGR0072240.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLE"
BASE COUNT          668 a          415 c          484 g          614 t
ORIGIN      
        1 tctatacggc aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt ataagaaaaa
       61 atcagtcccc taaaggagca gattatgaag ttattgtcta tcgccatttc tagctataat
      121 gcagcagcct atcttcatta ctgtgtggag tcgctagtga ttggtggtga gcaagttggg
      181 attttgatta tcaatgacgg gtctcaggat cagactcagg aaatcgctga gtgtttagct
      241 agcaagtatc ctaatatcgt tagagccatc tatcaggaaa ataaaggcca tggcggtgcg
      301 gtcaatcgtg gcttggcaga ggcttctggg cgctatttta aagtagttga cagtgatgac
      361 tgggtggatc ctcgtgccta cttgaaaatt cttgaaacct tgcaggaatt tgagagcaag
      421 ggtcaagagg tggatgtctt tgtgaccaat tttgtctatg aaaaggaagg tcagtcttgt
      481 aagaagagta tgagttacga ttcagtcttg cctgttcggc agatttttgg ctgggaccag
      541 gtcggaaatt tctccaaagg ccagtatatc atgatgcact cgctgattta tcggacagat
      601 ttgttgcgtg ctagccagtt ctaattgcct gaacatactt tttatgtcga taatctcttt
      661 gtctttacgc cccttcagca ggtcaagacc atgtactatc tgcctgtcga tttctatcgt
      721 tatttgattg ggcgtgagga ccagtctgtc aatgagcaag tgatgattaa gtgcattgac
      781 cagcaactca aggtcaatcg actcttggta gaccaacttt atttgtccca agtgagtcat
      841 cccaaaatgc gagaatatct gctgaatcat attgaaatca cgacggtgat ttccagtacc
      901 ctgctcaacc gatctggaac agcggagcat ctggcaaaaa aacgccaatt gtggacctat
      961 attcagcaga aaaatccaga agtctttcag gctattcgta agaccatgtt gagccgtttg
     1021 accaaacatt ctgtcttgcc agatcgcaaa ctgtccaatg tcgtctatca aatcaccaaa
     1081 tctgtttatg gatttaatta atataagtgt tttataagag ggatttaaga aaaattttaa
     1141 ctttttctta gtccttttta atttcaggag attatactag agtcatcaaa taaagaaaga
     1201 ctctaaggag aatcctatga aattcaatcc aaatcaaaga tatactcgtt ggtctattcg
     1261 ccgtctcagt gtcggtgttg cctcagttgt tgtggctagt ggcttctttg tcctagttgg
     1321 tcagccaagt tctgtacgtg ccgatgtggt caatccgacc ccaggtcaag tcttacctga
     1381 agagacatcg ggaacgaaag agggtgactt atcagaaaaa ccaggagaca ccgttctcac
     1441 tcaagcgaaa cctgagggcg ttactggaaa tacgaattca cttccgacac ctacagaaag
     1501 aactgaagtg agcgaggaaa caaactcttc tagtctggat acactttttg aaaaagatga
     1561 agaagctcaa gaaaatccag agctaacaga tgccttaaaa gaaactgtag atacagctga
     1621 tgtggatggg acacaagcaa gtccagcaga aactactcct gaacaagtaa aaggtggagt
     1681 gaaagaaaat acaaaagaca gcatcgatgt tcctgctgct tatcttgaaa aagctgaagg
     1741 gaaaggtcct ttcactgctg gtgtaaacca agtaattcct tatgaactat tcgctggtga
     1801 tggtatgtta actcgtctat tactaaaagc ttcggataat gctccttggt ctgacaatgg
     1861 tactgctaaa aatcctgctt tacctcctct tgaaggatta acaaaaggga aatacttcta
     1921 tgaagtagac ttaaatggca atactgttgg taaacaaggt caagctttaa ttgatcaact
     1981 tcgcgctaat ggtactcaaa cttataaagc tactgttaaa gtttacggaa ataaagacgg
     2041 taaagctgac ttgactaatc tagttgctac taaaaatgta gacatcaaca tcaatggatt
     2101 agttgctaaa gaaactgtcg aaaaagctgt taaagataac gttaaagaca gtatcgatgt
     2161 tccagcagcc tacctagaaa a
//
LOCUS       DBEJAQ010000096         2113 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.096, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000096 DBEJAQ010000000
VERSION     DBEJAQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.096"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(<1..265)
                     /locus_tag="ACL297_002051"
     CDS             complement(<1..265)
                     /locus_tag="ACL297_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGR0072241.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTDYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAA"
     gene            complement(210..392)
                     /locus_tag="ACL297_002052"
     CDS             complement(210..392)
                     /locus_tag="ACL297_002052"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGR0072242.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            727..>2113
                     /gene="pcpA"
                     /locus_tag="ACL297_002053"
     CDS             727..>2113
                     /gene="pcpA"
                     /locus_tag="ACL297_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HGR0072243.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGV"
BASE COUNT          758 a          303 c          338 g          714 t
ORIGIN      
        1 cagccgcaat ttcttcataa gtgcggtatt ctcgcacata ttgaagagtg gccataagaa
       61 ggtcttctag gcttaattta ggttttcgtc caccttttgc gtgtttaagt tgataatctg
      121 tttttaacac agctaacatc tctttaaaag tggtacgctg aacaccaaca agacgcttaa
      181 atcgtgtatc agttaattgt ttacttgctt cataatttcg cagggagtct attgactctt
      241 tggtaggtgt caatgttttt ttcatctata ccgagaatta ttttcccgcc atttgtattt
      301 gcaaatgctg agtaggtttc ccagaaagac tctggaagat tgtttttagc ttttttgtat
      361 tctaaatcaa ccccttcaaa ttttaagtcc atatttttcc tttacatctg ttttttgtgg
      421 ttctggtatt tgttcaagtt gagtgataat atagcgaatt gaatttcgag agtttttact
      481 cagttaattt cttttttaac ccgctttaat ttctttttta acacgtgtta aaaaagaaat
      541 taaagcgggt taatttttct tgacttaaaa tttaaaaatg atatactgtg tttatggaga
      601 gatgacaatt tataaaatgt actactctat tctactagat aatgattgaa taaaactttt
      661 tataacaacg gctagcaaag ttataatttt atatctatta gaaaataaat atgtaaggaa
      721 atttaaatga aaaaaactac aatattatca ttaactacag ctgcagttat tttagcagca
      781 tatgtcccta atgaaccaat cctagcagat actcctagtt cggaagtaat caaagagact
      841 aaagttggaa gtattattca acaaaataat atcaaatata aggttctaac tgtagaaggt
      901 aacataggca ctgttcaagt gggtaatgga gttactcctg tagagtttga agctggtcaa
      961 gatggaaaac cattcacgat tcctacaaaa atcacagtag gtgataaagt atttaccgtt
     1021 actgaagtag ctagtcaagc ttttagttat tatccagatg aaacaggtag aattgtctac
     1081 tatcctagct ctattactat cccatcaagc ataaaaaaaa tacaaaaaaa aggcttccat
     1141 ggaagtaaag ctaaaactat tatttttgac aaaggtagtc agctggagaa aattgaagat
     1201 agagcttttg atttttctga attagaagag attgaattgc ctgcatctct agaatatatt
     1261 ggaacaagtg cattttcttt tagtcaaaaa ttgaaaaagc taaccttttc ctcaagttca
     1321 aaattagaat taatatcaca tgaggctttt gctaatttat caaatttaga gaaactaaca
     1381 ttaccaaaat cggttaaaac attaggaagt aatctattta gactcactac tagcttaaaa
     1441 catgttgatg ttgaagaagg aaatgaatcg tttgcctcag ttgatggtgt tttgttttca
     1501 aaagataaaa cccaattaat ttattatcca agtcaaaaaa atgacgaaag ttataaaacg
     1561 cctaaggaga caaaagaact tgcatcatat tcgtttaata aaaattctta cttgaaaaaa
     1621 ctcgaattga atgaaggttt agaaaaaatt ggtacttttg catttgcaga tgcgattaaa
     1681 cttgaagaaa ttagcttacc aaatagttta gaaactattg aacgtttagc cttttacggt
     1741 aatttagaat taaaagaact tatattacca gataatgtta aaaattttgg taaacacgtt
     1801 atgaacggtt taccaaaatt aaaaagttta acaattggta ataatatcaa ctcattgccg
     1861 tccttcttcc taagtggcgt cttagattca ttaaaggaaa ttcatattaa gaataaaagt
     1921 acagagtttt ctgtgaaaaa agatacattt gcaattcctg aaactgttaa gttctatgta
     1981 acatcagaac atataaaaga tgttcttaaa tcaaatttat ctactagtaa tgatatcatt
     2041 gttgaaaaag tagataatat aaaacaagaa actgatgtag ctaaacctaa aaagaattct
     2101 aatcagggag tag
//
LOCUS       DBEJAQ010000097         2112 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.097, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000097 DBEJAQ010000000
VERSION     DBEJAQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.097"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            111..1457
                     /gene="gdhA"
                     /locus_tag="ACL297_002054"
     CDS             111..1457
                     /gene="gdhA"
                     /locus_tag="ACL297_002054"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGR0072244.1"
                     /translation="MTSAKEYIQSVFETVKTRNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGILYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDDRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          678 a          395 c          397 g          642 t
ORIGIN      
        1 aaaactgaat tgaaagaatt tttagaaaat ttctgttttt tctttacagt gaaaaaaaat
       61 tctgctataa tgtcttatgt aataaaatat gacaataaaa ggagaacgat atgacatctg
      121 ctaaagaata tatccaaagc gtgttcgaaa ctgtaaaaac tcgtaacggg cacgaggctg
      181 aattcctcca agctgttgaa gaatttttca acactttgga acctgtattt gaaaaacacc
      241 ctgagtatat cgaagaaaat atcttggcac gtattactga gcctgagcgc gtggtttctt
      301 tccgtgttcc ttgggttgac cgtgatggaa aaattcaagt aaaccgtggt taccgtgttc
      361 aattcaactc agctgttgga ccatacaaag gtggacttcg tttccaccca actgtaaacc
      421 aagggatctt gaaattcctc ggatttgaac aaatctttaa aaatgtctta actggacttc
      481 ctatcggtgg aggtaaaggt ggatcagact tcgatcctaa aggtaaaaca gatgctgaag
      541 tgatgcgctt ctgccaaagc ttcatgactg aattgcaaaa acacatcgga ccatcacttg
      601 acgtacctgc tggtgatatc ggtgttggtg gacgtgagat tggttacctt tacggtcaat
      661 acaaacgtct taaccaattt gatgctggtg tcttgactgg taaacctctt ggatttggtg
      721 gtagcttgat tcgtccagaa gcaactggtt acggtttggt ttactatact gaagaaatgc
      781 tcaaagctaa cggtaacagc tttgctggta agaaagtcgt tatttcaggt tctggtaacg
      841 ttgctcaata tgctcttcaa aaagcaactg aactcggtgc aactgttatt tctgtttctg
      901 actcaaatgg ttatgtcatc gatgaaaatg gtatcgactt cgatcttttg gttgatgtta
      961 aagaaaaacg tcgtgctcgt ttgactgagt atgcagctga aaaagcaact gcaacctacc
     1021 acgaaggtac tgtatggact tacgctggaa actatgacat tgctcttcca tgtgccactc
     1081 aaaacgaaat caacggtgaa gcagctaaac gtttggttgc tcaaggcgtt atctgtgtat
     1141 ctgaaggtgc caacatgccg agcgaccttg atgccatcaa agtttacaaa gaaaatggta
     1201 tcttatacgg acctgcaaaa gctgccaacg ctggtggtgt agccgtttca gcccttgaaa
     1261 tgagccaaaa cagccttcgc ctctcatgga ctcgtgaaga agttgatgac cgtctcaaag
     1321 acatcatgac aaacatcttc aacacagcta aaacaacttc agaaacatac ggtcttgata
     1381 aagactacct tgcaggagct aacattgctg cctttgaaaa tgtagcaaac gctatgattg
     1441 cccaaggtat tgtttaagat ttatttgctt aatcctcaat cactacgatt ggggattttt
     1501 agttgagtta gacagttggg aaattctaat taaataaaat aaaaactgaa caaattaatt
     1561 gcggaattca aaccaatttc taacatattt ctagaaatta atttgacttt tctaatcgat
     1621 ttgttcatat cttatttcaa tttactatac ttacttaacc ctttttatac tcaatgaaaa
     1681 tcaaagagca aactaggaag cgagtcgcag gctgtacttg agtacggcaa ggcaaagctg
     1741 acgtggtttg aagagatttt caaagagtat taaacaaaaa gaaaaaacgc tactcctaag
     1801 aatagcgatt taatcatgtt tttaagctac tactgtagtt gctctattat ttaagtgtta
     1861 tgaataactt caatatcttt aaagcttttt gttagattta taatttcaac tatagccatc
     1921 tctactcctt agtgtacagc aaaaaattat gcttaattct tattgttttt gtaaacaatc
     1981 agcgaaccca atactattcc attcaacaat cctaaagaaa taccatattc aactggaaaa
     2041 tctaaaaaaa ttgcaaacac tattccaatc atcaccccta gtgaggctgt tgttaaaatt
     2101 acatattcat tt
//
LOCUS       DBEJAQ010000098         1995 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.098, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000098 DBEJAQ010000000
VERSION     DBEJAQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.098"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="ACL297_002055"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="ACL297_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGR0072245.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1449..1995)
                     /locus_tag="ACL297_002056"
                     /pseudo
     CDS             complement(1449..1995)
                     /locus_tag="ACL297_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          610 a          398 c          415 g          572 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacatg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaacccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggttct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaaat ttcgtccttt ctttttgatg ttcagggcga
     1501 tgaaaatccg ttttttgaag ttttcaaagt tccgaaaacc aaaggcattg cgcttgataa
     1561 gtttgatgag attattggtc gcttccaatt tggcgtttga atagggtagt tgaagggcgt
     1621 tgacgatttt ctctttgtcc tttagaaagg ttttaaagac agtctgaaaa agaggatgaa
     1681 cctgcttaag attgtcctca atgagtccga aaaatttctc cggttcctta ttctgaaagt
     1741 gaaacagcaa gagttgatag agctgatagt ggtgtttcaa gtcttccgaa taactcaaaa
     1801 gcttgtttaa aatctcttta ttggttaagt gcatacgaaa agtaggacga taaaatcgct
     1861 tatcactcag tttacgacta tcctgttgta tgagcttcca gtagcgcttg atagccttgt
     1921 attcatgaga ctttcgatga aactgattca tgatttggac acgcacacga ctcatagtac
     1981 ggctaagatg ttgta
//
LOCUS       DBEJAQ010000099         1931 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.099, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000099 DBEJAQ010000000
VERSION     DBEJAQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.099"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..685
                     /locus_tag="ACL297_002057"
     CDS             <1..685
                     /locus_tag="ACL297_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGR0072246.1"
                     /translation="FNITGMNLISIITIFIQEPIRHIGTSLIGVIIIYSLGNMLWLFG
                     IHQAVIYSAILEPLLLINITENITAANNGQAIPHIINLSQIQTFALMGGSGSTLCLLI
                     ATFLVSRNAVSKNVAKLSFGPGIFNINEPVLFGYPIVYNISLAIPFITVPVLGILISY
                     LATVTGFMSPAFIQVPWTTPVFLNAWLATAGDVRAVLVQFIIFALGVLLYIPFIKVND
                     KVVEQEMEG"
     gene            690..998
                     /locus_tag="ACL297_002058"
     CDS             690..998
                     /locus_tag="ACL297_002058"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGR0072247.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            995..1303
                     /locus_tag="ACL297_002059"
     CDS             995..1303
                     /locus_tag="ACL297_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGR0072248.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT          659 a          328 c          328 g          616 t
ORIGIN      
        1 attcaatata actggaatga acttaatctc aatcattaca atctttattc aagaaccaat
       61 tcgtcatatt ggtacaagct taatcggggt cattattatt tactctttag gaaatatgtt
      121 atggctattt ggtattcacc aagcagttat ttacagtgcc atcctagaac cattactatt
      181 aattaacatt actgaaaaca tcactgcagc aaataatgga caagccattc cacacatcat
      241 caacctatca caaatacaaa cattcgcttt aatgggtggt agtggatcta cattatgttt
      301 attaatagca acattcttag tgagtcgcaa tgctgtctct aaaaacgtgg ctaaattatc
      361 ttttggacct ggtatcttca atatcaatga accagtatta ttcggttacc caatcgttta
      421 taacatttca ttagctattc catttatcac agttccagtc cttggtattt taatcagcta
      481 cttagcaaca gttacaggat tcatgagtcc tgcatttata caagttcctt ggactacacc
      541 agtattctta aatgcatggt tagcaacagc aggggacgtg agagcagttc tagttcaatt
      601 catcatcttt gcacttggag ttcttctata cattccattt atcaaagtta atgacaaagt
      661 tgttgaacaa gaaatggaag gttaagatta tgaaacaaat tttattagta tgtaatgcgg
      721 gaatgtcgac aagtatgctt gttaaaaaaa tgcaacaaag tgcgactgaa cgtgggattg
      781 aaatctctat ccaagcaaaa tcaatgacag aagctaaaaa gaatatccat gaagcagatg
      841 tcattctaat tgggccacaa attcgttatg agttacttgc tgtgaaagaa attgcaggaa
      901 atatccctgt agataccatt gatatgcggg attacggaat gatgaatgga gcaaaagttc
      961 tggaacaagc acttgcatgg ataggagaaa taagatgaca gaagaaatgg aatatatctg
     1021 ttttcagctc attgccaata gtggagcagc caaatcatcc tttattgaag ccatccaact
     1081 agctaaggca ggaaatttaa aagaagcgaa aattaaagtt gaagaagcgg aagactcctt
     1141 agtagaagcg cacaagattc actctaatct catacaaaaa gaagctactg gagaaaaaat
     1201 aggattctcc ttgctattta tgcatgcaga agaccaaatg gcttcaacag agattattca
     1261 gttgctatct aaagaattca tcgatctcta tcaaaataaa taggaacact gtcattccca
     1321 tctgtcttta ttaatgacga ctacattgta ggaacctcca aaagttggac aaaatgattg
     1381 aaaggattgg atcctgcatt acacaagatc caatcctttt cttttactct agtttttata
     1441 atatgttgaa tctagaatag tccactgtag tttctaaaaa attgctggaa atggatgtta
     1501 agctccaatt tatttgttta tatcttattt cagtttacta tactagctta ttattctagt
     1561 gaaaaattga atgagccact atttatttaa tatgtatgat gagggaatga ctcaaaaact
     1621 tcttgtcgat ataagaatat ctgagatagt attttaaaac gaaaggacta taaaagctca
     1681 tttccttcgc acttatttta tagagatcta tcattatact ctttagaaat caaaaagcaa
     1741 cacaccttgt catcgtttta tcgtaaatct gttaatgagg gggctagtct tatctgcaat
     1801 ctcaaatcag tgttttgagc tgaatggcac tcgttttcta gtttgctctt tgattttaat
     1861 tgggccttag taaatactac tcttaaatta aactatagtg aattgaaata agatgtgaac
     1921 aactctatca g
//
LOCUS       DBEJAQ010000100         1860 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.100, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000100 DBEJAQ010000000
VERSION     DBEJAQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.100"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(3..968)
                     /gene="xerA"
                     /locus_tag="ACL297_002060"
     CDS             complement(3..968)
                     /gene="xerA"
                     /locus_tag="ACL297_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HGR0072249.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="ACL297_002061"
     CDS             complement(1086..1499)
                     /locus_tag="ACL297_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGR0072250.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="ACL297_002062"
     CDS             complement(1496..1732)
                     /locus_tag="ACL297_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGR0072251.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          541 a          363 c          276 g          680 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagtctc
      241 tgggcttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtccgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctggta ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaacccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga gacttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcagata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
//
LOCUS       DBEJAQ010000101         1790 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.101, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000101 DBEJAQ010000000
VERSION     DBEJAQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.101"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            338..1689
                     /locus_tag="ACL297_002063"
     CDS             join(338..779,779..1689)
                     /locus_tag="ACL297_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGR0072252.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
BASE COUNT          609 a          274 c          392 g          515 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagggatt ctctgaagtt atggatgtcg ttccaatatg tgtacgttgg aatgttagtg
      121 cttatatggg gggaacagaa tcctctcttg attgaagaca agttagccat taggctggtt
      181 tgtctctttg tcaactgtag tgggttgata taatagtatt agtgagtggg ataaaagttt
      241 catttagttt attcagtaca aatttaacgg gtcaagattt attgaactgc accccaatag
      301 ttagacagaa aaaatctaac ttttggggtg ttttattatg aaattgagtt atgaagataa
      361 agttcagatc tatgaactaa gaaagcaagg acaaagcttc aaacagcttt caaaaagatt
      421 tggtgtggat gtttctggtc taaagtacat ggtgaaatta attgaccgtt atggcataga
      481 aatcgttaaa aaagggaaga atcgtcacta ttcttctaaa ttgaaacaag aaatgatgga
      541 taaagcctta ctggaaggtt gttcacaaag aagtattagt cttgattatg ccctcccaaa
      601 ccaaggaatg ctttcatttt ggccggcaca atacaagaaa aacgggtata ctattgttga
      661 gaaaacaaga gggagaccag ctaaaatggg acgtaaacgg aagaagactt gggaagaaat
      721 gacagaacta gaacgactcc aggaggagaa tgaacgctta cggactgagg tggcctacct
      781 aaaaaagtta aaagagttag aggaaaggga cgaagcccta gagcgagaaa ggcagagaca
      841 gttagagaaa tggtttcagg aggatttcga ctagattttc ttcttgaaac agcacgttta
      901 gctcgctcga cttactatta tcagttgaaa caactggatg gggttgataa agataaagag
      961 attaaaaccg aaattcaggg cattgataat gaacataaag gcaactatgg ctatcgtcga
     1021 atccatttag aactaaggaa tcgtggtttt gtggtcaatc ataagaaagt tcaacggttg
     1081 atgagaattc ttggtttaac ggctcgaatt cgccgtaaac ggaagtattc ttcctaccaa
     1141 ggagagattg gcaagaaagc agagaatctc attcaacgcc agttcgaagc atcgaggcca
     1201 atggaaaagt gctatacaga tgtgacagag tttgccattc caaatagcac acagaaactc
     1261 tatttatcac cagttttaga tggttttaac agtgaaatca ttgcttatca tctttctact
     1321 tcgcccaact tagaacaagt gaagagtatg ttagaacaag cctttacaga gaaatactat
     1381 gagaatacga ttctccatag tgaccaaggc tggcaatacc aacacgattc ttatcatcgg
     1441 ttcctagaga gtaagggaat tcaagcatcc atgtcacgca agggcaacag cccagacaac
     1501 ggcatgatgg aatctttctt tggcattttg aaatcggaga tgttttatgg ttatgagaag
     1561 aactttagat ctttagaaaa ccttgaacaa gctattgtgg actacattga ttactacaac
     1621 aacaaacgaa ttaaggtaaa actaaaagga cttagttctg tgcagtacag aactaaatcc
     1681 tttggataaa ttatttgtct aattttttgg ggtcagtaca gtattaaata ccatgatctt
     1741 atgcgtttta taataaaaaa tctgttcttg ttttattttt agaaaattta
//
LOCUS       DBEJAQ010000102         1705 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.102, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000102 DBEJAQ010000000
VERSION     DBEJAQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.102"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..86
                     /locus_tag="ACL297_002064"
     CDS             <1..86
                     /locus_tag="ACL297_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGR0072253.1"
                     /translation="PILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            134..1609
                     /locus_tag="ACL297_002065"
     CDS             134..1609
                     /locus_tag="ACL297_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGR0072254.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          478 a          345 c          345 g          537 t
ORIGIN      
        1 ggccaatctt attccgtatc ccagcaatga gcgctccagt atttgacaat attgaagcag
       61 ctattaaaga acatggatta agctaattct aatttgttaa cattcatata acataaaaaa
      121 gggaggaact gttatggatg caatctttga cctaatcgga aaggttttca atcccatctt
      181 agaaatgggt ggacctgtca tcatgttgat cattttgaca gtattggctt tactttttgg
      241 agtgaaattc tccaaagcgc ttgaaggtgg tatcaaactt gccatcgctc ttacaggtat
      301 cggtgctatc atcggtatgc taaatggtgc tttctcagca tcacttgcaa aattcgttga
      361 aaacactggt atccaattga gtattacaga cgttggttgg gcaccactcg caacaatcac
      421 ttggggttct gcttggacac tatacttctt gctcatcatg ttgattgtca acgtagtgat
      481 gcttgctatg aagaaaaccg atacacttga tgtcgatatc tttgatatct ggcacttgtc
      541 tatcacaggt ctcttgatta aatggtatgc tgataacaat ggtgtgagtc aaggggtttc
      601 actctttatt gctacagcag ctatcgtcct tgtcggtgtg ttgaaaatta tcaactctga
      661 cttgatgaaa cctacatttg atgaccttct taacgcccca agttcatcac caatgacatc
      721 aactcacatg aactacatga tgaacccagt tatcatggtt ttggataaga tttttgaaaa
      781 attcttccca ggccttgata aatatgactt tgatgctgct aaattgaaca agaaaatcgg
      841 tttctgggga tctaaattct tcatcggttt catccttggt atcgttatcg gtattatggg
      901 aactccacat ccaattgcag gtgttgcaga tgcagataaa tggcgtcttg ttatcaaagg
      961 atggttgtct cttggtttga ctgccggtgt atctttggaa ctcttctcac ttatcggttc
     1021 atggttcatc gcagccgtag aaccactatc acaaggtatt acaaacgttg ctactaaacg
     1081 tcttcgagga cgtaaattca atatcggtct tgactggcca ttcatcgctg gtcgtgctga
     1141 aatctgggct tgtgccaacg tacttgcacc aatcatgttg attgaagcag tgcttctttc
     1201 aaaagttgga aatggtatct tgccacttgc aggtatcatc gctatgggtg ttactccagc
     1261 tctcttggtt gtaactcgtg gtaaattgct ccgtatgatt atcttcggaa cactcttgtt
     1321 gccactcttc cttctttcag gtacacttat tgcaccattt gcaacagaac ttgctaaagg
     1381 tgtaggtgcc ttcccagaag gtgtgagcca aactcaattg attactcact ctactcttga
     1441 aggaccaatc gaaaaacttc ttggttggac aattggtaac actacaactg gtgatatcaa
     1501 agcaatcctt ggtgcagtag tcttccttgt attctatatc ggtatctttg cttggtacag
     1561 aaaacaaatg atcaaacgta acgaagagta cgcagcaaaa gcaaaataat gcgctcctat
     1621 aaaatataaa ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggttgctaaa
     1681 aacacagttt tgaggttgta gataa
//
LOCUS       DBEJAQ010000103         1703 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.103, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000103 DBEJAQ010000000
VERSION     DBEJAQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.103"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(84..692)
                     /locus_tag="ACL297_002066"
     CDS             complement(84..692)
                     /locus_tag="ACL297_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HGR0072255.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            870..1628
                     /locus_tag="ACL297_002067"
     CDS             870..1628
                     /locus_tag="ACL297_002067"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGR0072256.1"
                     /translation="MADYILVRDSEIVYETSSEIVSKVKVGEGTLNRFCKKIGYSGFQ
                     ELKLKMTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIELAIKLIREAN
                     QILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDLIIAFSLSGS
                     TKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGGSLMAKASQL
                     FIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT          676 a          170 c          277 g          580 t
ORIGIN      
        1 gttttttgaa attaaaatta ttttatcgtt gaaattagtt aaaaattttt ataaaatcaa
       61 gatgcaaggc tatgagatgt tctctagttt aatttttcat acttcgagaa tataaagtta
      121 tataaaaatg cattagcata ttttgttatt gagggaagta taaaaatcat taaagttatc
      181 caaactagta attgattctg taatataaag aagagtagaa ctgggataat ttcaataatt
      241 ctaataagaa cggcaataaa aaaatgttta taggctaaca tgattgaatt gtgtagagaa
      301 ttttttaaag tgttattata tctaccgatt aaaggaatta gaaatgttgt tgctattaaa
      361 tgaagtatga tatatattac tccaatatgt cgtaagatag aaatgatata tataggtaat
      421 ggtatgtagg gtaggaagaa caaactgaat aataagatta aaattggtaa attagatatt
      481 aatgctgctc ctaaattttt ccaataatct ttaaaaaaac tgtttgcgaa gtctctatag
      541 ggataggatt cctcgtgtcg tacaattttt attagagttg ctattagggc actttcacct
      601 gatccgatag caataactgt tactactgac gctacataga gtagatttaa cattataaga
      661 tttgaaactt tatcaataaa ttttatctgc atctttaact cctgctgttt ttatacttat
      721 aatatcacat catttgagtt tcatcaactt tgaatacatt aagaaagaat ataactatga
      781 ggaaaggaaa aaattattat gaataaatcc aaaaaatata aagaaagaat tcattatgaa
      841 tacgataatc ttactaaatc agagaaaaaa ttgctgacta tattttagtt agagatagtg
      901 aaatagttta tgaaacgtct tcagaaattg ttagtaaagt aaaagttgga gagggaactc
      961 ttaatcgatt ttgcaaaaag attggatact ctggttttca ggaactaaaa ttaaaaatga
     1021 cgaaagatat attagagtta gaatctcaaa aaatgagtag tgacacattt attgatgaaa
     1081 taaaaaataa ttatttatct attgtggaaa gtacaagaaa attaattgat ggtagacaaa
     1141 tagaattagc tataaaatta ataagagaag caaaccagat tttaatgatt ggtgtgggtt
     1201 ctagtggaaa tgcggctaga gaatttgaga gtagtttgtt aagaattgga ataataagta
     1261 agacggtaat tgatacccat tttcaactca tgcatactgc gttattgaaa gataatgatt
     1321 tgataatagc gtttagttta tctggttcca caaaagaagt tgaggaaact ttattaaacg
     1381 caaaaagaaa aaatgttaaa attatttcaa taacaaatta ttcgtccaga aatatagcaa
     1441 aattatctga ctgcgttctt ttgacatcaa aaaaagaatc ttatttagag ggtggtagtt
     1501 taatggctaa ggcttctcaa ctattcataa ttgatgtaat atgcactaga ctctctttaa
     1561 taaattatga agatactata tgtaaaaaag aagagattgc ttctttgctg tcaaataaag
     1621 tggaataaat caataatttt taaattttaa taaaaatttt ttaaaataaa ttaaaaaacg
     1681 atcctaaaag ggtcgttttt ttg
//
LOCUS       DBEJAQ010000104         1661 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.104, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000104 DBEJAQ010000000
VERSION     DBEJAQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.104"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            145..1491
                     /locus_tag="ACL297_002068"
     CDS             145..1491
                     /locus_tag="ACL297_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HGR0072257.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          491 a          359 c          325 g          486 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gctgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tattggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt
      481 tccagaactg acgaggaaac agtccatagt ttgcgatgcc tcaaccttga attggtcgaa
      541 ttctttttac agtttcacca gctaaaccaa ctcattgtag atatcgattc tacccatttc
      601 acaacttatg gcaagcaaga aggtgttgct tataacgccc actatcgtgc tcatggctat
      661 catcctcttt atgctttcga ggggaagaca ggttattgtt tcaatgccca gcttcgtcct
      721 ggtaatcgtt attgttctga agaggcagac agctttatca cacctgtttt agaacggttt
      781 aatcaacttc tctttcgaat ggatagtggc tttgcgaccc caaaattata cgatttaatt
      841 gaaaaaacag ggcaatacta cctcataaaa ctcaagaaaa atactgttct gagccgtctt
      901 ggagaccttt ccctcccttg cccacaggat gaggacttaa ccatcttgcc ccactccgcc
      961 tactcagaaa ctctctatca agcaggatct tggtcgcaca agcgtcgtgt ctgccagttc
     1021 tctgaacgaa aagaaggaaa cttgttctac gatgttattt ctctcgttac aaatatgacg
     1081 agtggaacaa gccaagacca gtttcagctt tatcgtggac gtggtcaagc cgagaatttc
     1141 atcaaggaga tgaaggaggg attttttggc gataaaacgg atagttcaac cttaatcaaa
     1201 aacgaagttc gtatgatgat gagctgtatc gcctacaatc tctatctttt tctcaaacat
     1261 ctagctggag gtgacttcca aactttaaca atcaaacgct tccgccatct ttttcttcac
     1321 gtggtaggaa aatgtgttcg aacaggacgc aagcagctcc tcaaattgtc tagtctctat
     1381 gcctattccg aattgttttc agcactttat tctaggatta gaaaagtcaa cctgaatctt
     1441 cctgttcctt atgaaccacc tagaagaaaa gcgtcgttaa tgatgcatta aagaacagtc
     1501 gagatgaaaa aatcgtgtga cgaaccaagg gaggagtctg cccttttgag gaaatctagc
     1561 gaggaaaaac gatactggaa cagcagaaag taaaactgac ctcatgagga ggaagaaagt
     1621 ggctcatgag gtcaggggtt ttgtaagtta catctagttg a
//
LOCUS       DBEJAQ010000105         1609 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.105, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000105 DBEJAQ010000000
VERSION     DBEJAQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.105"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            35..491
                     /locus_tag="ACL297_002069"
                     /pseudo
     CDS             35..491
                     /locus_tag="ACL297_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            656..955
                     /locus_tag="ACL297_002070"
     CDS             656..955
                     /locus_tag="ACL297_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HGR0072258.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="ACL297_002071"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="ACL297_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGR0072259.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          552 a          321 c          282 g          454 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     1201 gaaaagtatt gtactatgat gattcaacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgataca aggaatcgat tcactggctt atctcgttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggatactat ataaacgctt tgagaacgga
     1441 aaactgactt ggcccagtac agaaaaggat gtcaaagctc tcgcacctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatc
//
LOCUS       DBEJAQ010000106         1559 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.106, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000106 DBEJAQ010000000
VERSION     DBEJAQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.106"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(1..886)
                     /locus_tag="ACL297_002072"
                     /pseudo
     CDS             complement(1..886)
                     /locus_tag="ACL297_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730289.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(867..1061)
                     /locus_tag="ACL297_002073"
     CDS             complement(867..1061)
                     /locus_tag="ACL297_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGR0072260.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(1231..>1559)
                     /gene="tnpB"
                     /locus_tag="ACL297_002074"
     CDS             complement(1231..>1559)
                     /gene="tnpB"
                     /locus_tag="ACL297_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGR0072261.1"
                     /translation="VYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQVFLFCGGRKDR
                     FKALYWDGQGFWLLYKHKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFSITPKINLS
                     ESRDFY"
BASE COUNT          445 a          337 c          288 g          489 t
ORIGIN      
        1 gataaaactg actgacgccg gcaccaagca aagaaatctt ccattagggg ttggagatgt
       61 tcttgacgtt tctgtagtcg ttcatcagct ggcaaagcct cccagtttct ttccaaggaa
      121 aataactgat tacaataagc taaaccttta gctcctaagg atgatttatt cgcttgcttg
      181 ggggtcgctt caaaaaactt ccttctcaca tgtacccaac atccaacaag ttcagcttct
      241 tccagttgac aataagcttc tagcggttcg cagtttacca agcttagcgt cgccttgctc
      301 ctaaaccttg gactgctatc gcataggcag aactagagga ctaaagtcct aagttactgc
      361 cgcaacatat cacaatgcac atagccagaa taatctccta ggaattcttg cactaccgaa
      421 ccactccgac gctgatcatg gtggtacagc gtgattgctt gattctcagc tttcccagac
      481 aaaaaggtcc aatagtaggt cagatggcta tcattctcta gaacccgata ggaggtttca
      541 tctgcatgaa gtaagggctg aatcaatagt ctcgttataa aggggcttta ggaataggag
      601 cttttacaat tttatcactt ggattattct tactgcatgt ttggcatttg taagcgtgtt
      661 ggatatgatc cactcttttt aattgcgcag gaataaagac caattcttgt cgttgaaggc
      721 tcgctccaat ctcttttaaa tcgtcctgac aatcagggca aatgctctct tctacttgat
      781 gatgaacttc ttctgaatca aattgggcaa gaatagcttg acgtttccct ttagatttct
      841 tacgtttgta ggtaatttcc tctgtttcaa ctgggaacat ctccatcttt ctcagatgga
      901 gactcttcct caaaaaggtt gagttgtcca aaagggcaaa cacttttctc agaggatttt
      961 ccagagagct tttgcgttag ataagccact tgttcacaca gaagggaaag ttcattagtg
     1021 agactatcaa ttatagcact ctgttgttga atgattttca atcttctccc atagactttc
     1081 ctccttattt cctaacttta ttatatagtg tattgaatct ataacagtac accttgactg
     1141 ctaaaatatt tctataaatt aatttgactt tcctgataga gatgttcata tcttatttca
     1201 attcactata ctaaaaagaa agtcctcatt tcaatagaaa tcacgacttt ctgataaatt
     1261 tattttggga gtgatagaaa agcccttcat aagccaatct acttgttcag gtgtgagagc
     1321 tttgacatcc ttttctgtac tgggccaagt cagtctgccg ttctcaaagc gtttatgttt
     1381 atatagtagc caaaatcctt gaccatccca gtaaagggct ttaaagcggt ctttacgtcc
     1441 accacaaaag agaaaaactt gaccggagaa aggatccaat tcaaagtggg ttttaaccag
     1501 ataagccagt gaatcgattc cttgtctcat atcagtcttc ccacacacga gatagacct
//
LOCUS       DBEJAQ010000107         1285 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.107, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000107 DBEJAQ010000000
VERSION     DBEJAQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.107"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..661
                     /locus_tag="ACL297_002075"
     CDS             <1..661
                     /locus_tag="ACL297_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGR0072262.1"
                     /translation="FYLNKGKVYGFVGPNGAGKTTIIKMILGILKPDSGKITIFNQTV
                     EQNSENILSRIGLVLGPSFYGHLDAYKNLKLIANMKGLSLDTERLNEYLSMVGLKDVK
                     KKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPINGLDPQGVIEIRSLIRFLQEKKGI
                     TFLISSHILSELDKVISDIIIINYGKVEFFGSCHYLLQKYNCRNLEEAYLACLAGGEY
                     D"
     gene            654..>1285
                     /locus_tag="ACL297_002076"
     CDS             654..>1285
                     /locus_tag="ACL297_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGR0072263.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNVLLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVT"
BASE COUNT          447 a          145 c          208 g          485 t
ORIGIN      
        1 attttattta aataagggaa aagtatatgg ttttgttgga ccaaatgggg ctggaaaaac
       61 tacaattatc aaaatgattc tagggatttt aaaaccagat tctgggaaaa taactatttt
      121 taatcaaacc gtagaacaga attccgaaaa tatattatct aggataggat tagttttggg
      181 accttcattt tatggtcatc ttgatgctta taaaaatttg aagttaatag caaatatgaa
      241 agggcttagt ttagatactg aaagacttaa tgaatatcta tcaatggtag gattaaaaga
      301 tgttaaaaag aaaaaagtga aaaatttttc aatgggcatg aaacaacgtc taagtatagc
      361 tgcatcattg ttaggtagtc cagaaatatt aatttgggat gaaccaatca atggacttga
      421 tccacagggt gtaattgaaa ttagaagttt aatccgtttt ctacaggaga aaaaaggtat
      481 tacattttta atatcaagtc atattcttag tgaactggat aaagtgatat ctgatattat
      541 aattataaat tatgggaaag ttgaattttt tggttcttgc cactaccttt tacaaaagta
      601 taattgtcga aatctggaag aagcctactt agcttgttta gctggaggtg aatatgatta
      661 aattagagtt tctaaagcag aaaaaaagca tcttatggtt tgttcttatt tttccaataa
      721 ttctgaatgt tttgttatat atagacttaa cttttaggta tcgaggttat ttattggtgc
      781 atcaaaatga acttgcttta tctaattggc agttaatttt taaagaacaa acaatatttt
      841 atttttctga actattttat ttagttctat ctttaataat ttacgaagtg tttgcagtag
      901 aatttaaaaa tgatgcatgg ttaaccgtaa tttcattgcc atttagaaac aagtacacaa
      961 taaattctaa attactcatt acagtggttt acacatttac tttttggttg tctgattata
     1021 ttagcttata cgttattggg aaggctattg ataacagttt agagattggt ttgattttct
     1081 ttttaaaaac atttaccatt caattaattt cctcgttgat gataatgtta ttatattttc
     1141 taactttagt acttataagg aaaatttctg gaataatacc aattggtatt ataatgatga
     1201 ttttaacaat tagcatatac tacaacgatt ataattttaa gatttactta ccatttactt
     1261 atctttctca tgcttttaga gtgac
//
LOCUS       DBEJAQ010000108         1192 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.108, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000108 DBEJAQ010000000
VERSION     DBEJAQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.108"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            complement(1..224)
                     /locus_tag="ACL297_002077"
                     /pseudo
     CDS             complement(1..224)
                     /locus_tag="ACL297_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
     gene            complement(484..795)
                     /locus_tag="ACL297_002078"
     CDS             complement(484..795)
                     /locus_tag="ACL297_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HGR0072264.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(810..>1192)
                     /locus_tag="ACL297_002079"
     CDS             complement(810..>1192)
                     /locus_tag="ACL297_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HGR0072265.1"
                     /translation="ATGFYYGIPSENEQLFGARRKIPEYKFVAEVVSYDDAVQTATIR
                     QRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIKVPQPVQSGD
                     MVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          370 a          238 c          203 g          381 t
ORIGIN      
        1 tagtgaatag actccatacg tccttcgatt tttagactgt ccacaccatt ttcaatcata
       61 tctgagatat ggtcaatcat agacatatca acggctgaca ttgaaaattc ttctggaatc
      121 tcaccctgca aactcttacg ttctttccca aatggcatat cgtaaaggtc gtacttccaa
      181 cggcatgact gagaacatcc accacggttg gcatcacgca tactctagac ggattgtttt
      241 gacaattcta accttttaga ctttgcataa taactgttaa acagtcgaaa attaccccag
      301 ttttaacaag tagtcaaaat accagataaa gtaaaaaggg gctaggaaaa aagtctttaa
      361 aatcttaaaa acgcatagta tcaggtattg aacaatcttg atattatgcg ttttattgtg
      421 ggaagattta cttcattttc tcttggaatt gagttttctc ctacttcaaa actagatgat
      481 ttgctattta aaatagcgtt ccttatgatt ttggcaacct ggattaaagc gagaaaaaca
      541 aaagaggcaa cttaagcttt ctttatattg cttatatgtt aatagtttta gacaaacacc
      601 acataaaata ggcttatcct gtatcaaaga taagggataa ggctcaaaag ggtgattttc
      661 taatgaatca tgacaccgat aacaagcata atacttttta caatcatagc attgtaaagc
      721 aataatatcc agcttgctat ggtaatgaat gcatcttgtt tgcttatcca ctaagaaacc
      781 ctgtgcctct atcatttcct tttcctttct taagctcgaa ctgtgacgct ggttccatct
      841 tccttataaa aattgatgag tccttctttt aatgcacgaa ccatgtctcc tgattgaacg
      901 ggttgtggga ctttaatagt caatagttcc attggatttg gagcgcggtc gattttattt
      961 cctttggcat catgcaaatc ttcaatatag gtttcaaaat gacggaaacc tggaccataa
     1021 aactcaactt ggtccccttc gttaatgacg tttcgttgac gaatggttgc tgtttgtacc
     1081 gcatcatcat aagaaaccac ttcagcgaca aacttgtact caggaatttt acggcgagca
     1141 ccaaacaact gctcattttc agatggtata ccgtagtaga aacctgttgc ca
//
LOCUS       DBEJAQ010000109         1184 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.109, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000109 DBEJAQ010000000
VERSION     DBEJAQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.109"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..>1184
                     /locus_tag="ACL297_002080"
     CDS             <1..>1184
                     /locus_tag="ACL297_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HGR0072266.1"
                     /translation="LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQ
                     TAEELASRIASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDY
                     VRISFDVTFPVALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSED
                     YQTNNVFIRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQ
                     RADSLEAGVSRLTEGLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEV
                     RAGSIHQEILNATKDKASKSELTQTAEELSSKIASVQASGRNLFLNSLFKQDISKTGI
                     WTTSTYTAAIDSESKYLGHNALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQ
                     DV"
BASE COUNT          346 a          248 c          287 g          303 t
ORIGIN      
        1 acttagtcgg actaagaatg aactggctgg cgtgaagtca gcgcaagcga cgtatgagga
       61 gacgacgact cgtagactgt cagaactgac caacttggcc aatggtaaag ccagcaagtc
      121 agaactcacg caaacagctg aggagctggc tagtcggatt gcgagtgtgc aggcaggtag
      181 ttcacggaat tacttcagga attcacgttc aagaacgttc acaacaggag gtcaagcggt
      241 atacgactat cgaacattca tagttcctga tttctggaag aacagtgaca ggttcaagcg
      301 tgattatgtt cgcatatctt ttgatgtgac tttccctgtc gccctagtaa atgacatgcc
      361 tgctatggtg cattttagtg ctcatccatg gtatgcctac agaaacttaa tttttaaagg
      421 tggaactgtc gaacgccaac attttgagtt tacgattgac ttgtctagtt cttctgagga
      481 ctatcagact aataatgtgt tcattcgttt tggtactaat tatggatttc ctgctggtct
      541 gcaggtcgtc attgagaacg ctatgttatc ggttggtaat tattttccag cctatcaacc
      601 agcgtatgaa gaccaagaag accgtgtctc agtagtcgaa tccaacttta agcagcgtgc
      661 tgattcactc gaagctggtg taagccgtct gactgaaggc cttagaacca aagcggatat
      721 cagctcactc aatgtgactg ctgagaatat ccggcaatct gtgaagagcc ttgagacaga
      781 cacgcagaac aagctaaatc agaagttgag tcaggctgaa tttgaggtgc gagccggctc
      841 tatccatcag gaaatcctga acgcaaccaa ggataaagcc agcaagtcag aactcacgca
      901 gacagctgag gagctctcta gtaagatagc gagtgtgcag gcatccggtc gaaatctatt
      961 cttgaactca ctattcaagc aggatatttc aaaaacagga atttggacaa cgagtacata
     1021 tacggctgct atcgatagcg aaagtaagta tcttggacat aacgctctta aaattatagg
     1081 tctgaatcca tctggccgtg atggagggaa tcccaaggtt acttatccag ctctgggtca
     1141 attcgggaaa gtaattcccg gaagtacgac taatcaagat gtaa
//
LOCUS       DBEJAQ010000110         1169 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.110, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000110 DBEJAQ010000000
VERSION     DBEJAQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.110"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            180..>1169
                     /locus_tag="ACL297_002081"
     CDS             180..>1169
                     /locus_tag="ACL297_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGR0072267.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNY"
BASE COUNT          355 a          192 c          268 g          354 t
ORIGIN      
        1 tagaaaaaga aaggagtttt cgcaattttt tgaaaaaata ttgatttaca attgaacgag
       61 taagtgaact tgttgtagat tttcaaaata attaaagaag tatagtaatt tacttgaatc
      121 ttctctaaaa aggtagtata ataaaaacat agaaaacgct tacaggagaa ggggagtata
      181 tggataatag agaagcatta aaaaccttta tgacgggtga aaatttttat ctccaacatt
      241 atctaggagc acatagggaa gaactaaatg gagagcatgg ctataccttc cgtgtttggg
      301 cacctaatgc tcaggctgtt cacttggttg gtgattttac caactggatt gaaaatcaga
      361 ttccaatggt aagaaatgat tttggggtct gggaagtctt taccaatatg gctcaagaag
      421 ggcatattta caaatatcat gtcacacgtc aaaatggtca tcaactgatg aagattgacc
      481 cttttgctgt caggtatgag gctcgtccag gaacaggggc aatcttaaca gagcttcgtg
      541 agaagaaatg gaaggatgga ctttggctgg cacgaagaaa acgttggggc tttgaagagc
      601 gtcctgtcaa tatttatgaa gttcacgctg gatcatggaa aagaaattct gatggcagtc
      661 cttatagttt tgcccagctc aaggatgaac tcattcctta tctcgttgaa atgaactata
      721 ctcatattga gtttatgccc ttgatgtccc atcctttggg cttgagttgg gggtatcagc
      781 ttatgggtta cttcgcttta gagcatgctt atggccgacc agaggagttt caagattttg
      841 tcgaggagtg tcatacccat aatattgggg ttattgtgga ctgggtacca ggtcacttta
      901 ccatcaacga tgatgcctta gcctattatg atgggacacc gacttttgaa taccaagacc
      961 ataataaggc tcataaccat ggttggggtg cccttaattt tgaccttgga aaaaatgaag
     1021 tccagtcctt cttaatttct tgcattaagc attggattga tgtctatcat ttggatggta
     1081 ttcgtgtgga tgctgttagc aacatgctct atttggacta tgatgatgct ccatggacac
     1141 ctaataaaga tggcggaaat ctcaactat
//
LOCUS       DBEJAQ010000111         1062 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.111, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000111 DBEJAQ010000000
VERSION     DBEJAQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.111"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            100..1062
                     /locus_tag="ACL297_002082"
                     /pseudo
     CDS             100..1062
                     /locus_tag="ACL297_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          329 a          165 c          230 g          338 t
ORIGIN      
        1 ttttatgagg ctcttttgtc ctgctcttgt ttcaattgac tatagttcac ggatacagtt
       61 gggaaagaag ctattaaata tttggagagt ccttttggga tgaacatgtc aaatattact
      121 atagatgggc aagaaaagat gggcaagaaa tggatctgag tcaaacgatg cttcttatgc
      181 tcaagtagtg agtctttatg atgatacaag tatctctgtt agtaataatg agacggacaa
      241 agtgttgact ggatccctct atactgaaac aaatgaacag ggattaacga ttccaagcag
      301 tttactaaaa aattggaatg aacagacagg aaaaaatttg acaactaatg atcttattgg
      361 caaatcagtc tcagccagca ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt
      421 tcaaacgaag attgtacgtg taatcaatga tgaagatgac atggaggaca gcaacagttt
      481 catgctgtct catcaaatgg aaacgatttt gaaagaggct ggatttacga aagctgtatc
      541 ttattttatc ttggaactca aagatccatc acagacaaaa gtagtaacag aagaattaca
      601 gaaaaataag aagtatactg tgctttctca acagagagtt cttgatattg tgattacctt
      661 tattcgtgtt attcagggat tattgattgt gctttcatca caagctattg tggtagcagc
      721 ggttatgatt ggtatcatta tttacatcaa tatcatgcaa cgttccaagg aaataggtgt
      781 catgaaagca gttggttatc agaatcgtgg tgtcaaagga atttttattt acgaggctat
      841 ctggattgta ggcatcgcct tgctgctggc atttttggtt gcacaagggg tgggaagttt
      901 ggcgaatgcg attgtaagtc acttttaccc atccatcact aaggtttttg aattaaatct
      961 tttatctgtt ttaggaactc tagttttcgc tctattactt ggttatgtct cagcctactt
     1021 cccggcgcgt aagattagta aaatggatcc tgtagaatcg tt
//
LOCUS       DBEJAQ010000112          894 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.112, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000112 DBEJAQ010000000
VERSION     DBEJAQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.112"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            231..>894
                     /locus_tag="ACL297_002083"
     CDS             231..>894
                     /locus_tag="ACL297_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HGR0072268.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QAL"
BASE COUNT          319 a          154 c          186 g          235 t
ORIGIN      
        1 taccttagtc aagaattaga tgtttcacta tgtttgagta agttgatgat ttcatttgat
       61 aacaggtttg aaactgttag gctaggtggc caaggctaat catagccttg gtttagctga
      121 aaaaacaggt tcaagggttc ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca
      181 cctctgtgct atacttgttg ttcaccacaa acacaaggaa aggcacagag atgcaagaac
      241 attatacccc aaaagggaaa catttgacaa tagataaccg tcgcttgatt gagcggtgga
      301 agaatgaaaa taagtccaat cgtgaaattg caggcttgtt aggaaaggcg cctcaaacga
      361 ttcataatga agtcaaaaga ggtacaactt tacaacaagt gagaaaaggg ctatacaaaa
      421 aggtctattc tgccgattac gcacaaactg tttaccaatt caatcgaaaa cggtcggtga
      481 aaaagttaat tttaacaaag gaaatcagag agaagatctt acactatcat aagcaaaaat
      541 tttcgcctga aatgatggtt aacaagaagc aagtgaaagt tggtatttca accatctact
      601 actggtttca taatggtcat ttaggattga cgaaggccga catgctttat cccagaaaaa
      661 ggaaaggtgt caagaagcaa gctagtccga actttaagcc ggcaggtaaa tctatcgaag
      721 aacgtcctga cgttattaat cttcgcttgg aaaatggtca ttatgaaatt gataccgtcc
      781 tactgactaa gataaaaaat tattgcctgt tagtcttaac cgaccggcgg agcagacacc
      841 aaattataag gttaattcca aataaaactg ctgaatctgt caatcaggcg ctta
//
LOCUS       DBEJAQ010000113          851 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.113, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000113 DBEJAQ010000000
VERSION     DBEJAQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.113"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..>851
                     /locus_tag="ACL297_002084"
     CDS             <1..>851
                     /locus_tag="ACL297_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HGR0072269.1"
                     /translation="PYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQIPRI
                     INQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTK
                     GKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYDLAK
                     QLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQDSRKL
                     SDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGLIED
                     NLKQVHP"
BASE COUNT          261 a          186 c          139 g          265 t
ORIGIN      
        1 tcccttacct cgaaacaact ggtatgcctt ctagaattct ccttagaaaa cgccgtttca
       61 agtgctatca ctgttcaaaa atgatggttg ctgagactcc cctggtaaag aaaaatcacc
      121 aaatccctcg tatcatcaac caaaaaattg ctcaaaagtt aattgaaaaa atttctatga
      181 ctgatattgc ccatcagctg gccatttcaa cttcaactgt cattcgcaag ctcaatgact
      241 ttcactttga gcatgatttt tctcggcttc caaagattat gtcttgggat gagtatgcct
      301 tcactaaggg aaagatgagt ttcattgcgc aagattttga taatcttaat attatcactg
      361 ttcttgaagg cagaacacaa gctgtcatcc gaaatcactt tcttcgctac gatagagccg
      421 ttcgttgtca agtgaaaatc attacgatgg atatgtttag tccttactat gacttggcta
      481 aacagctttt tccgtgtgct aaaatcgttc tagatcgttt ccatattatc caacatctca
      541 gccgtgccat gagtcgtttt cgtgttcaaa ttatgaatca gtttgaacga aaatctcatg
      601 aatacaaggc tatcaagcgt tactggaaac tcatccaaca ggatagtcgt aaactcagcg
      661 ataaacgttt ttatcgccct acttttcgca tgcacttaac aaataaagaa attcttgaca
      721 agattttaag ctattcagaa gacttgaaac accactatca gatctatcaa ctcttacttt
      781 ttcactttca gaacaaagac cctgagaaat ttttcggact cattgaggac aatctgaagc
      841 aggttcatcc t
//
LOCUS       DBEJAQ010000114          657 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.114, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000114 DBEJAQ010000000
VERSION     DBEJAQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.114"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            1..657
                     /locus_tag="ACL297_002085"
                     /pseudo
     CDS             1..657
                     /locus_tag="ACL297_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          268 a           99 c          128 g          162 t
ORIGIN      
        1 tgataaagtt cagatctatg aacttagaaa acaaggatat agcttagaga agctttcaaa
       61 taaatttggg ataaataatt ctaatcttag atatatgatt aaattgattg atcgttacgg
      121 aatagagttc gtcaaaaaag gaaaaaatcg ttactattct cctgatttaa aacaagaaat
      181 gattaataaa gtctgacatg aaggctggac taaagacaga gtttctcttg aatacggtct
      241 cccaagtcgt acgatacttc ttaactggct agcacaatac agaaaaaaac gggtatacta
      301 ttgttgagaa aacaagaggg agagtaccta aaatgggacg taagccaaaa aagagacctg
      361 aagagaggac agaattagaa catcttcaag cagaaaatga gtacctgaga gcggagaatg
      421 ccatcctaaa aaagttaaga gaactccgat tgaaggagga aaaagagaaa gaagaaagac
      481 agaaattgtt caagaattaa tgactgagtt ttcgttagat attcttctaa aagccattaa
      541 actagctcgt tcgacctact actatcactt gaaacagcta gacaaaccag ataaggacca
      601 agagcttaaa gctgaaattc aatccatttt tatcgaacac aagggaaatt atgctta
//
LOCUS       DBEJAQ010000115          640 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.115, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000115 DBEJAQ010000000
VERSION     DBEJAQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.115"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..144
                     /locus_tag="ACL297_002086"
     CDS             <1..144
                     /locus_tag="ACL297_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGR0072270.1"
                     /translation="KERSKVENILAKVKTLKMFSTTYRNHRKRFGLRMNLIVGIINHE
                     LGF"
     gene            167..640
                     /locus_tag="ACL297_002087"
                     /pseudo
     CDS             167..640
                     /locus_tag="ACL297_002087"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          246 a           83 c          118 g          193 t
ORIGIN      
        1 aaggagagaa gcaaggttga gaacatcctt gccaaagtaa aaacgcttaa aatgttttca
       61 acaacctatc gaaatcatcg taaacgcttc ggattacgga tgaatttgat tgttggtatt
      121 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatcaaaaa taccatcagg
      181 attatataag attgatacag gaaaagtttt attttatggt ataaatatta atcaaataga
      241 taaaaaaata ttaagtcaaa atttaggagt agttccacag gattcgtttt tattgaacag
      301 aagtattctt gataacataa ctttaaagca cgaagttact tcacaaaaga tagaggaagt
      361 ttgtaaagca gttcaaatct atgatgaaat catggctatg ccgatgaaat ttaatactat
      421 catctcagag atgggttcaa atatttcagg tggtcaaaga caacggatag cactggcacg
      481 tgcattaata aataatccta gtattgtaat tttagatgaa gcaactagtg ctttagacac
      541 tattaatgag gaaagaataa caaagtatat aaaaagtcag ggctgtactc aaataattgt
      601 agctcataga ttgtcaacga ttaaggatgc ggatattatt
//
LOCUS       DBEJAQ010000116          621 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.116, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000116 DBEJAQ010000000
VERSION     DBEJAQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.116"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            1..471
                     /locus_tag="ACL297_002088"
                     /pseudo
     CDS             1..471
                     /locus_tag="ACL297_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            509..>621
                     /locus_tag="ACL297_002089"
     CDS             509..>621
                     /locus_tag="ACL297_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGR0072271.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAV"
BASE COUNT          203 a          103 c          123 g          192 t
ORIGIN      
        1 attagattat ttctttgaca aaaaccttgt gttttgctta gaagcggata atcaagaata
       61 actctttgat caggtagcaa gcttgttgga ggaacgagaa attgtaactc caacttatcg
      121 tgaagctttg atcacgcgtg aaaagtcatt tccaactggt ttagatatgg aatttctagg
      181 aaaggacttg caaatgtaat gattcctcac acagatattg ttcataatct agctgagaaa
      241 gtggtggttg ttcgattaga gaaaccggtg actttccata atatgattgc gccaggtaag
      301 gaagtagaag tatccttgct cttctttatc atcaataatt caagttcaag tcaaacgaac
      361 attcttgctc agttgatgga ctttttcaca ggaaatggac atcttgaaga cctatcaaaa
      421 atttccgaac cagaaaaact ttatgcttat atagatgaag caatcgctta atcttgtcta
      481 ttaaaaaata aaatcggagg aagtctatat gattaaaatt cttgctgcct gcggtgcagg
      541 tgttaactca agtcaccaaa ttaaaagtgc tctagaagaa gaactttcaa accgtggtta
      601 cgatgttcac tgtgatgcag t
//
LOCUS       DBEJAQ010000117          618 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.117, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000117 DBEJAQ010000000
VERSION     DBEJAQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.117"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            <1..468
                     /locus_tag="ACL297_002090"
     CDS             <1..468
                     /locus_tag="ACL297_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGR0072272.1"
                     /translation="DYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREALIT
                     REKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVE
                     VSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            506..>618
                     /locus_tag="ACL297_002091"
     CDS             506..>618
                     /locus_tag="ACL297_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGR0072273.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAV"
BASE COUNT          204 a          109 c          115 g          190 t
ORIGIN      
        1 gattatttct ttgacaaaaa ccttgtgttt tgcttagaag cggataatca agaacatctc
       61 tttgatcagg ttgcaacttt attggaagaa cgagaaattg taactccaac ttatcgtgaa
      121 gccttgatca cgcgtgaaaa gtcatttcca actggtttag atatggaatt tctaggaaag
      181 gacttgccaa atgtagcgat tcctcacaca gatattgttc ataatctagc tgagaaagtg
      241 gtggttgttc gattagaaaa accagtaact tttcacaata tgatagctcc tgataaggaa
      301 gtagaagtat ctctactctt ctttatcatt aacaattcaa gttcaagtca aacgaacatt
      361 ctggctcagt tgatggactt tttcacagga aatggtcatc ttgaagacct atcaaaaatt
      421 tctgaaccag aaaaacttta tgcttacatt gctgaagcaa ccgcttaatc ttgtctatta
      481 aaaaataaaa tcggaggaaa tccaaatgat taaaattctt gctgcctgcg gtgcaggtgt
      541 taactcaagt caccaaatta aaagtgctct agaagaagaa ctttcaaacc gtggttatga
      601 tgttcactgt gatgcagt
//
LOCUS       DBEJAQ010000118          615 bp    DNA     linear   BCT 22-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 680159
            SAMN38261860-rid24843163.denovo.118, whole genome shotgun sequence.
ACCESSION   DBEJAQ010000118 DBEJAQ010000000
VERSION     DBEJAQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38261860
            Sequence Read Archive: SRR26833923
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002576307.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002576307.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2025 09:00:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 57 of 121
            Pseudo Genes (incomplete)         :: 57 of 121
            Pseudo Genes (internal stop)      :: 46 of 121
            Pseudo Genes (multiple problems)  :: 33 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38261860-rid24843163.denovo.118"
                     /strain="GPSC16"
                     /isolate="680159"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2019"
                     /collected_by="UKHSA"
     gene            105..584
                     /locus_tag="ACL297_002092"
     CDS             105..584
                     /locus_tag="ACL297_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGR0072274.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          230 a           80 c          141 g          164 t
ORIGIN      
        1 gtctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattatcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aagcaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ctagtaagtc atgcaaaaaa
      601 aaatgaaaaa aatta
//
